Growth of chikungunya virus in baby hamster kidney cell (BHK-21-clone 13) suspension cultures.
This report describes the methods used to obtain high titers of chikungunya virus with suspension cultures of BHK-21-clone 13 cells. The cells were grown at 37 C to a cell concentration of 10(6) to 2 x 10(6) per ml. After maximum cell growth, the cells were inoculated with chikungunya virus at a multiplicity of 1 to 2 50% suckling mouse intracerebral lethal doses (SMICLD(50)) per cell in the spent Eagle's minimum essential medium for suspension cultures (MEMS), or the cell cultures were centrifuged at 200 x g and resuspended in either fresh MEMS or medium 199 prior to inoculation. The medium used had no effect on virus titer. The inoculated cultures were incubated at 34 C until the cell viability dropped to 30%, which usually occurred 28 to 30 hr postinoculation. After these procedures, chikungunya virus titers of log(10) 10.3 to 11.8 SMICLD(50) per ml were obtained.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002646:Chikungunya virus / Q000254:growth & development / Q000302:isolation & purification*; D002999:Clone Cells / Q000254:growth & development; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques; D007106:Immune Sera; D007668:Kidney; D051379:Mice; D014357:Trypsin; D014776:Virus Cultivation; D014779:Virus Replication
D003470:Culture Media; D007106:Immune Sera; D014357:Trypsin