title: Nature and origins of phosphorus compounds in isolated cell walls of Staphylococcus aureus.
abstract: Preparations of purified cell walls from Staphylococcus aureus were shown to contain small amounts of phospholipid and glycerol teichoic acid. Since these are components of the cell membrane, it is probable that the wall itself contains no lipid, but does retain fragments of membrane because of physical connections between wall and membrane. In walls of S. aureus strain 52A5, which completely lacks ribitol teichoic acid, the only phosphorylated compound identified as a genuine wall component was a phosphorylated derivative of murein that gave rise to muramic acid phosphate on acid hydrolysis. Muramic acid phosphate was also identified in hydrolysates of walls from S. aureus H and strain 52A2.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D001329:Autolysis; D001426:Bacterial Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000032:analysis; D002473:Cell Wall / Q000032:analysis / Q000201:enzymology; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004586:Electrophoresis; D005227:Fatty Acids / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D005994:Glycerophosphates / Q000032:analysis; D006027:Glycosides; D006358:Hot Temperature; D006868:Hydrolysis; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000032:analysis; D009113:Muramidase; D009696:Nucleic Acids / Q000032:analysis; D010209:Paper; D010428:Pentosephosphates; D010743:Phospholipids / Q000032:analysis; D010759:Phosphorus Isotopes; D011108:Polymers; D012266:Ribose; D013210:Staphylococcus / Q000032:analysis / Q000166:cytology* / Q000201:enzymology; D014166:Transferases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D005227:Fatty Acids; D005994:Glycerophosphates; D006027:Glycosides; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D009696:Nucleic Acids; D010428:Pentosephosphates; D010743:Phospholipids; D010759:Phosphorus Isotopes; D011108:Polymers; D012266:Ribose; D014166:Transferases; D000217:Acyltransferases; D009113:Muramidase; D005990:Glycerol