title: Regulation of the pathway for the degradation of anthranilate in Aspergillus niger.
abstract: Studies were carried out to determine the factors governing the induction of anthranilate hydroxylase and other enzymes in the pathway for the dissimilation of anthranilate by Aspergillus niger (UBC 814). The enzyme was induced by growth in the presence of tryptophan, kynurenine, anthranilate, and, surprisingly, by 3-hydroxyanthranilate, which was not an intermediate in the conversion of anthranilate to 2,3-dihydroxybenzoate. There was an initial lag in the synthesis of anthranilate hydroxylase when induced by tryptophan, anthranilate, and 3-hydroxyanthranilate. Cycloheximide inhibited the enzyme induction. Comparative studies on anthranilate hydroxylase, 2,3-dihydroxybenzoate carboxy-lyase, and catechol 1:2-oxygenase revealed that these enzymes were not coordinately induced by either anthranilate or 3-hydroxyanthranilate. Structural requirements for the induction of anthranilate hydroxylase were determined by using various analogues of anthranilate. The activity of the constitutive catechol oxygenase was increased threefold by exposure to anthranilate, 2,3-dihydroxybenzoate, or catechol. 3-Hydroxyanthranilate did not enhance the levels of catechol oxygenase activity.
mesh_terms: D001230:Aspergillus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D003124:Colorimetry; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D003653:Decarboxylation; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D007737:Kynurenine / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D012459:Salicylates / Q000378:metabolism; D013053:Spectrophotometry; D014364:Tryptophan / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001565:Benzoates; D002396:Catechols; D003470:Culture Media; D012459:Salicylates; D062367:ortho-Aminobenzoates; D007737:Kynurenine; D014364:Tryptophan; D003513:Cycloheximide; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D002262:Carboxy-Lyases