Head proteins from T-even bacteriophages. II. Physical and chemical characterization.
Three classes of structural head proteins, having molecular weights of 43,000, 18,000, and 11,000 daltons, were isolated from the T-even bacteriophages and were characterized. Based on electrophoretic studies, the 43,000-dalton class contained one major protein and one (or two) minor components, the 18,000-dalton class contained two protein components, and the 11,000-dalton class contained one major component. The N-terminal residues for the 43,000- and the 11,000-dalton classes were alanine, and the N-terminal residues for the 18,000-dalton class were methionine and alanine. Of the three classes of proteins, the 18,000-dalton proteins were the most acidic, whereas the 11,000-dalton proteins were the most basic. The amino acid composition of the 11,000-dalton class revealed that methionine and cysteine were absent and lysine, histidine, and tryptophan content was higher in the 11,000-dalton class than in the other two classes of proteins. Estimates of the relative number of the three classes of structural proteins were made and indicated that there were between 1,600 and 2,000 subunits of the 43,000-dalton proteins, 100 to 200 of the 18,000-dalton proteins, and 1,000 to 1,500 of the 11,000-dalton proteins. Evidence was presented that the 43,000-dalton proteins and the 11,000-dalton proteins readily formed aggregates with themselves but not with each other. The significance of these interactions to the structure of the T-even phage head was discussed.
D000409:Alanine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D001322:Autoanalysis; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000032:analysis*; D004589:Electrophoresis, Disc; D006868:Hydrolysis; D007525:Isoelectric Focusing; D008715:Methionine / Q000032:analysis; D008970:Molecular Weight; D012972:Sodium Hydroxide; D013395:Sucrose; D013460:Sulfur Isotopes; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification
D000596:Amino Acids; D013460:Sulfur Isotopes; D014764:Viral Proteins; D014316:Tritium; D012972:Sodium Hydroxide; D013395:Sucrose; D008715:Methionine; D000409:Alanine