The accumulation of ( 14 C)5-hydroxyindol-3-ylacetic acid by the rabbit choroid plexus in vitro.
1. The lateral and fourth ventricular choroid plexuses of the rabbit have been shown to be capable of the in vitro accumulation of 5-hydroxyindol-3-ylacetic acid (5-HIAA).2. The accumulation of 5-HIAA occurs against a concentration gradient and is dependent on metabolic energy.3. It is possible to inhibit the accumulation of 5-HIAA by the presence of the dopamine metabolite, 3-methoxy-4-hydroxyphenylacetic acid (HVA) or of probenecid in the incubation medium.4. It would appear that the characteristics of the accumulation of 5-HIAA by the isolated choroid plexuses are consistent with the involvement of this tissue in the in vivo active transfer of 5-HIAA from the c.s.f. to the blood.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002247:Carbon Isotopes; D002831:Choroid Plexus / Q000187:drug effects / Q000378:metabolism*; D003864:Depression, Chemical; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010648:Phenylacetates / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011817:Rabbits
D002247:Carbon Isotopes; D010648:Phenylacetates; D006897:Hydroxyindoleacetic Acid; D011339:Probenecid