Competence-inducing factor of Bacillus stearothermophilus.
An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture medium but is not infected with TP-1C phage DNA, even in the presence of CF. The production of CF by the wild type or Ton-r mutant and the ability of the wild type or Com(-) mutant to react with CF does not occur after these cultures have grown at 67 C for 1 hr or longer. A preliminary characterization of the CF is described. The autolytic enzyme or the temperate phage of the wild type and the Ton-r and Com(-) mutants do not have competence-inducing activity.
D001407:Bacillus / Q000254:growth & development*; D001435:Bacteriophages; D003470:Culture Media; D004279:DNA, Viral / Q000494:pharmacology*; D003851:Deoxyribonucleases / Q000494:pharmacology; D005374:Filtration; D005827:Genetics, Microbial; D006358:Hot Temperature; D009154:Mutation; D010206:Papain / Q000494:pharmacology; D013997:Time Factors; D014170:Transformation, Genetic; D014357:Trypsin / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology
D003470:Culture Media; D004279:DNA, Viral; D014361:Trypsin Inhibitors; D003851:Deoxyribonucleases; D014357:Trypsin; D010206:Papain