Uptake and incorporation of amino acids and peptides by Bacteroides amylophilus.
Bacteroides amylophilus H-18 demonstrated a higher growth yield, a slightly higher growth rate, and a diminished lag period when Tryptose was added to the basal medium. This uptake of labeled amino acids was concentration-dependent, as the contribution of exogenous amino acid to the cell protein increased from 15.4 to 24.1% when the concentration of Casamino Acids in the medium was increased from 1.4 to 2.8 mg/ml. There was considerable redistribution of (14)C-label to other amino acids. Tryptic peptides of casein competed effectively with the amino acids for uptake. The (14)C-label from a protein was incorporated into B. amylophilus H-18 cells presumably after breakdown of the protein by the B. amylophilus H-18 protease.
D000596:Amino Acids / Q000378:metabolism*; D001322:Autoanalysis; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D001439:Bacteroides / Q000032:analysis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001693:Biological Transport, Active; D002241:Carbohydrates / Q000494:pharmacology; D002247:Carbon Isotopes; D002364:Caseins / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003470:Culture Media; D005374:Filtration; D008320:Maltose / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010461:Peptones; D011492:Protein Hydrolysates; D014357:Trypsin; D014364:Tryptophan / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002241:Carbohydrates; D002247:Carbon Isotopes; D002364:Caseins; D003470:Culture Media; D010455:Peptides; D010461:Peptones; D011492:Protein Hydrolysates; D008320:Maltose; D014364:Tryptophan; D010447:Peptide Hydrolases; D014357:Trypsin