Production of substituted L-tryptophans by fermentation.
Claviceps purpurea has been shown to produce extracellular l-tryptophan from indole in stirred fermentors. The substrate specificity of this conversion was investigated by using substituted indoles, anthranilic acid, and 4-chloro-anthranilic acid. Addition of 2-, 4-, 5-, 6-, and 7-methyl indole or 6-chloroindole to C. purpurea C1M produced the corresponding substituted l-tryptophan. In contrast, addition of l-methyl, 6-trifluoromethyl, 6-nitro-, or 4-benzyloxy-substituted indoles, or anthranilic and 4-chloroanthranilic acids did not produce detectable amounts of the corresponding tryptophan.
D001203:Ascomycota / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003470:Culture Media; D005285:Fermentation; D007211:Indoles / Q000378:metabolism; D014364:Tryptophan / Q000032:analysis / Q000096:biosynthesis*; D062367:ortho-Aminobenzoates / Q000378:metabolism
D003470:Culture Media; D007211:Indoles; D062367:ortho-Aminobenzoates; D014364:Tryptophan