Cell surface antigens: serial sectioning of single cells as an approach to topographical analysis.
The topographical distribution of H-2 antigens on the surfaces of C57BL/6 mouse thymocytes and lymph-node lymphocytes was investigated by a new technique. Single cells were reacted with visually labeled antibody directed against H-2 antigen. Each cell was processed for electron microscopy individually and was serially sectioned. Models constructed from serial electron micrographs provided detailed views of the entire cell surface and showed that H-2 antigen occurs on thymocytes in small isolated regions and on lymphocytes in large interconnected regions.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology*; D006648:Histocompatibility; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D010942:Plant Viruses / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000941:Antigens