Incorporation of radioactive seleno-(75Se)-methionine into mumps virus.
Mumps virus was grown in embryonated chicken eggs in the presence of radioactive seleno-((75)Se)-methionine. Virus in the allantoic and amniotic fluids was concentrated in a sucrose density gradient, and a peak of viral material coincided with a significant peak of (75)Se-radioactivity. The radioactivity was acid-insoluble and remained associated with the virus after purification by erythrocyte adsorption and elution and centrifugation on a second sucrose density gradient. After amino-acid hydrolysis of the radioactive virus, only (75)Se-methionine was recovered by chromatographic analysis. These results demonstrate that the radioactive (75)Se-methionine was incorporated into protein of infectious mumps virus.
D000327:Adsorption; D000653:Amniotic Fluid; D000818:Animals; D001826:Body Fluids; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002645:Chickens; D002854:Chromatography, Paper; D004912:Erythrocytes; D005321:Extraembryonic Membranes; D006386:Hemagglutination Tests; D008715:Methionine / Q000378:metabolism*; D009109:Mumps virus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D009439:Neuraminidase; D011868:Radioisotopes; D012643:Selenium; D013395:Sucrose; D014733:Vibrio / Q000201:enzymology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D011868:Radioisotopes; D014764:Viral Proteins; D013395:Sucrose; D008715:Methionine; D009439:Neuraminidase; D012643:Selenium