The isolation of polysomes from rat liver. Contamination by membrane proteins with high affinity to ribosomes.
D000327:Adsorption; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm; D004586:Electrophoresis; D004589:Electrophoresis, Disc; D004721:Endoplasmic Reticulum / Q000187:drug effects*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008566:Membranes; D009963:Orotic Acid / Q000378:metabolism; D009994:Osmolar Concentration; D011189:Potassium Chloride; D011485:Protein Binding; D014176:Protein Biosynthesis; D011506:Proteins; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D012270:Ribosomes; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology; D014316:Tritium
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D011506:Proteins; D013501:Surface-Active Agents; D014316:Tritium; D009963:Orotic Acid; D012313:RNA; D011189:Potassium Chloride; D007930:Leucine