title: Cholestasis: lamellar structure of the abnormal human serum lipoprotein.
abstract: An abnormal lipoprotein was visualized directly in serum by electron microscopy of preparations negatively stained with potassium phosphotungstate. It appears as a unique disk-shaped particle with major axis measuring 400 to 600 angstroms and minor axis measuring about 100 angstroms. Chemical analysis, viscosity measurements, and x-ray diffraction analysis of purified preparations indicate that the particle, consisting of a one-to-one molar mixture of cholesterol and choline phosphatides associated with a small amount of protein, is a flattened vesicle, the wall of which is a continuous lipid bilayer.
mesh_terms: D001796:Blood Protein Disorders / Q000097:blood*; D002779:Cholestasis / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D004952:Esters / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008854:Microscopy, Electron; D010743:Phospholipids / Q000097:blood; D010772:Phosphotungstic Acid; D013046:Specific Gravity; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation; D014783:Viscosity; D014961:X-Ray Diffraction
chemical_list: D004952:Esters; D008074:Lipoproteins; D010743:Phospholipids; D014280:Triglycerides; D010772:Phosphotungstic Acid; D002784:Cholesterol