title: The accumulation of ( 14 C)5-hydroxyindol-3-ylacetic acid by the rabbit choroid plexus in vitro.
abstract: 1. The lateral and fourth ventricular choroid plexuses of the rabbit have been shown to be capable of the in vitro accumulation of 5-hydroxyindol-3-ylacetic acid (5-HIAA).2. The accumulation of 5-HIAA occurs against a concentration gradient and is dependent on metabolic energy.3. It is possible to inhibit the accumulation of 5-HIAA by the presence of the dopamine metabolite, 3-methoxy-4-hydroxyphenylacetic acid (HVA) or of probenecid in the incubation medium.4. It would appear that the characteristics of the accumulation of 5-HIAA by the isolated choroid plexuses are consistent with the involvement of this tissue in the in vivo active transfer of 5-HIAA from the c.s.f. to the blood.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002247:Carbon Isotopes; D002831:Choroid Plexus / Q000187:drug effects / Q000378:metabolism*; D003864:Depression, Chemical; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010648:Phenylacetates / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011817:Rabbits
chemical_list: D002247:Carbon Isotopes; D010648:Phenylacetates; D006897:Hydroxyindoleacetic Acid; D011339:Probenecid