Petite mutation in yeast. II. Isolation of mutants containing mitochondrial deoxyribonucleic acid of reduced size.
A series of petite mutants of Saccharomyces cerevisiae, generated after treatment for various times with ethidium bromide, was isolated, and the mitochondrial deoxyribonucleic acid size for each member was estimated. It was found that, as the treatment time with ethidium bromide was increased, the mitochondrial deoxyribonucleic acid isolated from the petite series was increasingly reduced in size.
D000225:Adenine; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004247:DNA / Q000032:analysis; D005827:Genetics, Microbial; D008928:Mitochondria / Q000032:analysis / Q000187:drug effects; D008970:Molecular Weight; D009153:Mutagens; D009154:Mutation; D010617:Phenanthridines; D012440:Saccharomyces / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification*; D013395:Sucrose; D014316:Tritium
D002247:Carbon Isotopes; D002712:Chlorides; D009153:Mutagens; D010617:Phenanthridines; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D004247:DNA; D000225:Adenine