Ribosomal competence and spore germination in Fusarium solani.
Extracts prepared from macroconidia of Fusarium solani f. sp. phaseoli are capable, under defined conditions, of incorporating phenylalanine into polypeptide with exogenous polyuridylic acid as messenger. Extracts from ungerminated and germinated spores have approximately the same activity. With endogenous template, leucine incorporation occurs, but in this reaction extracts from germinated spores have about 10 times more activity than do those from ungerminated spores. It is suggested that the low rate in ungerminated spores is attributable to a relative deficiency in the number of ribosomes which are organized into polysomes.
D002247:Carbon Isotopes; D002474:Cell-Free System; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D003513:Cycloheximide / Q000494:pharmacology; D005374:Filtration; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D007930:Leucine / Q000378:metabolism; D008274:Magnesium; D008623:Mercaptoethanol; D010649:Phenylalanine / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism*; D013170:Spores / Q000378:metabolism*; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D013698:Templates, Genetic; D014238:Trichloroacetic Acid; D014461:Ultracentrifugation
D002247:Carbon Isotopes; D011119:Polynucleotides; D010649:Phenylalanine; D014238:Trichloroacetic Acid; D008623:Mercaptoethanol; D012343:RNA, Transfer; D003513:Cycloheximide; D012260:Ribonucleases; D007930:Leucine; D008274:Magnesium