Agrobacterium tumefaciens ribonucleic acid synthesis in tomato cells and crown gall induction.
Purified Agrobacterium tumefaciens deoxyribonucleic acid (DNA) does not produce crown gall tumors in growing plants, conditioned by wounding, as the living bacteria do. Purified bacterial DNA migrates in the plant and replicates, but it is not transcribed in our experimental conditions. On the contrary, when DNA is released naturally from bacteria into plant cells, a bacterial ribonucleic acid (RNA) can be found in these cells. There seems to be a direct relation between the appearance of A. tumefaciens RNA in the plant cells and the induction of the tumor.
D001345:Autoradiography; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D005815:Genetic Code; D005827:Genetics, Microbial; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D010941:Plant Tumors / Q000209:etiology*; D010944:Plants / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002712:Chlorides; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D002586:Cesium; D012313:RNA; D004247:DNA; D013936:Thymidine; D012293:Rifampin; D014529:Uridine