Formation of saliva and potassium transport in the perfused cat submandibular gland.
1. In the cat submandibular gland perfused with modified Locke solutions, salivary secretion during acetylcholine (ACh) infusion and K uptake from the perfusion fluid after the ACh-induced K loss were measured.2. Strophanthin G (10(-5)M) abolished K uptake, whereas salivary secretion was unaffected.3. Ethacrynic acid (10(-4)-2 x 10(-4)M) hardly affected K uptake whereas salivary secretion was severely inhibited.4. During perfusion with Ca-free solution K uptake was unaffected, whereas salivary secretion was severely reduced.5. The presence or absence of CO(2)/HCO(3) in the perfusion fluid was of no importance for the secretory process and the K uptake. Acetazolamide (2 x 10(-4)-10(-3)M) did not inhibit these transport processes.6. It is suggested that two kinds of Na transport occur in the acinar cells of the salivary glands: a Na extrusion coupled with K uptake, responsible for the maintenance of the concentration gradients across the cell membrane; and a Na transport coupled with Cl transport into the acinar lumen, responsible for the formation of the primary secretion.
D000086:Acetazolamide / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology; D002415:Cats; D002462:Cell Membrane / Q000378:metabolism; D003864:Depression, Chemical; D004976:Ethacrynic Acid / Q000494:pharmacology; D010477:Perfusion; D011188:Potassium / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects; D012964:Sodium / Q000378:metabolism; D013328:Strophanthins / Q000494:pharmacology; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*
D001639:Bicarbonates; D013328:Strophanthins; D002245:Carbon Dioxide; D012964:Sodium; D004976:Ethacrynic Acid; D000109:Acetylcholine; D000086:Acetazolamide; D011188:Potassium; D002118:Calcium