Preparation of highly labeled (32P)nucleic acids from yeasts; isolation of "denaturable" leucine acceptor transfer RNA.
Culture conditions were developed that permit efficient incorporation of [(32)P]phosphate into nucleic acids of baker's yeast. RNA sufficiently labeled for sequence determination was obtained in this way. Pure "denaturable" leucine acceptor transfer RNA (tRNA(Leu)(3)) was isolated for this purpose.
D002848:Chromatography, DEAE-Cellulose; D004589:Electrophoresis, Disc; D007930:Leucine / Q000378:metabolism; D008722:Methods; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000096:biosynthesis*; D010710:Phosphates / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012343:RNA, Transfer / Q000032:analysis / Q000302:isolation & purification*; D012440:Saccharomyces / Q000378:metabolism*
D009696:Nucleic Acids; D010710:Phosphates; D010759:Phosphorus Isotopes; D012343:RNA, Transfer; D007930:Leucine