Synthesis of 5S and 4S RNA in metaphase-arrested HeLa cells.
The continued synthesis of both 5S and 4S RNA in metaphase-arrested HeLa cells is demonstrated; 5S RNA is apparently synthesized at approximately 74 percent of the interphase rate, while 4S RNA is synthesized at approximately one-third the rate. The ratio of uridine incorporation to RNA methylation is used to correct for the alteration in the specific activity of the pyrimidine pool during metaphase arrest.
D002247:Carbon Isotopes; D002455:Cell Division; D006367:HeLa Cells / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008938:Mitosis; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D012334:RNA, Neoplasm; D014316:Tritium; D008715:Methionine; D014529:Uridine