title: Regulation of tryptophan biosynthetic enzymes in Neurospora crassa.
abstract: The formation of enzymatic activities involved in the biosynthesis of tryptophan in Neurospora crassa was examined under various conditions in several strains. With growth-limiting tryptophan, the formation of four enzymatic activities, anthranilic acid synthetase (AAS), anthranilate-5-phosphoribosylpyrophosphate phosphoribosyl transferase (PRAT), indoleglycerol phosphate synthetase (InGPS), and tryptophan synthetase (TS) did not occur coordinately. AAS and TS activities began to increase immediately, whereas PRAT and InGPS activities began to increase only after 6 to 12 hr of incubation. In the presence of amitrole (3-amino-1,2,4-triazole), the formation of TS activity in a wild-type strain was more greatly enhanced than were AAS and InGPS activities. With a tr-3 mutant, which ordinarily exhibits an elevated TS activity, amitrole did not produce an increase in TS activity greater than that observed on limiting tryptophan. With tr-3 mutants, the increased levels of TS activity could be correlated with the accumulation of indoleglycerol in the medium; prior genetic blocks which prevented or reduced the synthesis of indoleglycerol also reduced the formation of TS activity. The addition of indoleglycerol to cultures of a double mutant (tr-1, tr-3) which could not synthesize indoleglycerol markedly stimulated the production of TS activity but not PRAT activity; the production of TS activity reached the same level with limiting or with excess tryptophan. A model explaining these and other related observations on enzyme formation in N. crassa is proposed.
mesh_terms: D002474:Cell-Free System; D002845:Chromatography; D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids / Q000378:metabolism; D004794:Enzyme Repression; D005470:Fluorometry; D005827:Genetics, Microbial; D005990:Glycerol / Q000096:biosynthesis / Q000494:pharmacology; D007211:Indoles / Q000096:biosynthesis / Q000494:pharmacology; D007535:Isomerases / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism*; D008956:Models, Chemical; D009154:Mutation; D009491:Neurospora / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D013053:Spectrophotometry; D014230:Triazoles / Q000494:pharmacology; D014364:Tryptophan / Q000096:biosynthesis* / Q000494:pharmacology; D014461:Ultracentrifugation; D062367:ortho-Aminobenzoates / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids; D007211:Indoles; D014230:Triazoles; D062367:ortho-Aminobenzoates; D014364:Tryptophan; D009713:Nucleotidyltransferases; D007535:Isomerases; D008025:Ligases; D005990:Glycerol