Effects of poly-L-lysine on infectious viral nucleic acid.
Infectious ribonucleic acids (IRNA) of Venezuelan equine encephalitis and Eastern equine encephalitis viruses were observed to form noninfectious complexes with a basic polyamino acid, poly-l-lysine. Original infectivity was recovered from the complexes by digestion of the polylysine with Pronase, and partial recovery was effected by treatment with sodium dodecyl sulfate. Infectivity could not be recovered from the complexes containing polylysine of 100,000 molecular weight by changes in ionic strength, pH, or by treatment with phenol, deoxycholate, or digitonin. Masking of infectivity by polylysine was demonstrated in vivo as well as by plaque assay in tissue culture. Poly-l-lysine preparations of high molecular weight (44,000 to 100,000) were more effective than low molecular weight (3,000) materials in masking infectivity of IRNA. When complexes, in which infectivity had been masked by low molecular weight polylysine, were suspended in 1 m NaCl, some infectivity was recovered. Complexes of polylysine-IRNA differed from control IRNA alone in (i) resistance to inactivation by ribonuclease, (ii) sedimentation patterns in sucrose gradient centrifugation, and (iii) stability of recoverable infectivity during different physical treatments.
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002645:Chickens; D003080:Cold Temperature; D046508:Culture Techniques; D004071:Digitalis Glycosides / Q000494:pharmacology; D004666:Encephalitis Virus, Venezuelan Equine / Q000254:growth & development; D004668:Encephalitis Viruses / Q000254:growth & development; D005347:Fibroblasts; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000494:pharmacology; D010636:Phenols; D011073:Polyamines / Q000494:pharmacology; D012367:RNA, Viral / Q000302:isolation & purification / Q000494:pharmacology* / Q000528:radiation effects; D012964:Sodium; D013302:Streptomyces; D013395:Sucrose; D013431:Sulfates; D014461:Ultracentrifugation; D014466:Ultraviolet Rays; D014776:Virus Cultivation
D001647:Bile Acids and Salts; D004071:Digitalis Glycosides; D010636:Phenols; D011073:Polyamines; D012367:RNA, Viral; D013431:Sulfates; D013395:Sucrose; D012964:Sodium; D010447:Peptide Hydrolases; D008239:Lysine