Properties of an extracellular  -galactosidase secreted by Neurospora crassa.
An extracellular beta-galactosidase with an activity optimum at about pH 4 was found to occur in the filtrates of Neurospora crassa strain 74A when this mold was grown on certain sugars. This activity accounted for a substantial portion of the beta-galactosidase activity in the culture. The beta-galactosidase in the medium appeared to be a secreted, extracellular enzyme, not a product of cell lysis. The extracellular activity was found to have physical and kinetic properties similar to those of an intracellular beta-galactosidase previously found in Neurospora. Some conditions for the production and concentration of the enzyme are described.
D050260:Carbohydrate Metabolism; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003124:Colorimetry; D003470:Culture Media; D004586:Electrophoresis; D004790:Enzyme Induction; D005374:Filtration; D005696:Galactosidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009491:Neurospora / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose
D003470:Culture Media; D005782:Gels; D007202:Indicators and Reagents; D013395:Sucrose; D005696:Galactosidases