Salt extraction of soluble HL-A antigens.
Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.
D000941:Antigens / Q000302:isolation & purification*; D046508:Culture Techniques; D004586:Electrophoresis; D006648:Histocompatibility; D006650:Histocompatibility Testing; D006982:Hypertonic Solutions; D008214:Lymphocytes / Q000276:immunology; D008722:Methods; D011189:Potassium Chloride; D012995:Solubility; D014461:Ultracentrifugation
D000941:Antigens; D006982:Hypertonic Solutions; D011189:Potassium Chloride