Visual pigment fluorescence.
The fluorescence of cattle rod outer segments (dried) and of rhodopsin in solution lies in the range of 575 to 600 millimicrons with a quantum efficiency of 0.005 if excitation is in the visible band near 500 millimicrons. The emission is abolished by bleaching at -196 degrees C but can be reversibly regenerated by irradiation with light of longer wavelength (600 millimicrons). This behavior reflects the known interconversion of rhodopsin to prelumirhodopsin at this temperature.
D000818:Animals; D002417:Cattle; D005453:Fluorescence; D005470:Fluorometry; D010786:Photoreceptor Cells; D012168:Retinal Pigments; D013057:Spectrum Analysis
D012168:Retinal Pigments