Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.
1. The chromatography of rat small-intestinal beta-galactosidase activities on gel-filtration and ion-exchange columns has been studied. Five different substrates were used to measure beta-galactosidase activity (lactose, phenyl beta-galactoside, o-nitrophenyl beta-galactoside, p-nitrophenyl beta-galactoside and 6-bromo-2-naphthyl beta-galactoside) and the activity was measured at one acid and one more neutral pH value. 2. By gel filtration one acid beta-galactosidase, hydrolysing lactose and the hetero-beta-galactosides at about the same rate, and one more neutral beta-galactosidase, hydrolysing lactose much more rapidly than the hetero-beta-galactosides, were separated. 3. By ion-exchange chromatography the acid enzyme was fractionated into two components. These may be individual enzymes or different forms of the same enzyme.
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005696:Galactosidases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D007527:Isoenzymes; D007785:Lactose; D009596:Nitrophenols; D010636:Phenols; D051381:Rats; D014461:Ultracentrifugation
D007527:Isoenzymes; D009596:Nitrophenols; D010636:Phenols; D005696:Galactosidases; D007785:Lactose