Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.
A sensitive method for detecting cells containing antibody to beta-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.
D000818:Animals; D000937:Antigen-Antibody Reactions; D005452:Fluoresceins; D005470:Fluorometry; D005696:Galactosidases; D006027:Glycosides; D007118:Immunoassay; D051379:Mice; D010783:Photometry; D013154:Spleen / Q000166:cytology / Q000201:enzymology / Q000276:immunology*
D005452:Fluoresceins; D006027:Glycosides; D005696:Galactosidases