Hydrocortisone requirement for the induction of glutamine synthetase in chick-embryo retinas.
Hydrocortisone has been found to induce glutamine synthetase activity in chick-embryo retinas in culture. Evidence is presented to show that the hydrocortisone is definitely required for transcription; its requirement for translation has not been ruled out. The possible identity of hydrocortisone with an active component of calf-serum diffusate reported earlier is discussed. The data also indicate that the glutamine synthetase messenger RNA is stable for at least several hours.
D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003864:Depression, Chemical; D004355:Drug Stability; D004790:Enzyme Induction; D006854:Hydrocortisone / Q000494:pharmacology; D008025:Ligases / Q000378:metabolism; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012160:Retina / Q000187:drug effects / Q000201:enzymology; D013053:Spectrophotometry
D002247:Carbon Isotopes; D003470:Culture Media; D012333:RNA, Messenger; D011691:Puromycin; D012313:RNA; D003513:Cycloheximide; D008025:Ligases; D006854:Hydrocortisone