Purification of large quantities of influenza virus by density gradient centrifugation.
New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration and isopycnic banding of the virus in a buffered sucrose gradient. Kilogram quantities of impurity have been rapidly separated from grams of purified virus, which have been conveniently concentrated several hundred-fold by the purification process. Experimental vaccines made by these procedures are being evaluated.
D000818:Animals; D001466:Barium Sulfate; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D046508:Culture Techniques; D006387:Hemagglutination, Viral; D007252:Influenza Vaccines / Q000302:isolation & purification; D008722:Methods; D051379:Mice; D009975:Orthomyxoviridae / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D014776:Virus Cultivation
D007252:Influenza Vaccines; D001466:Barium Sulfate