Phosphorylation of choline and ethanolamine in Ehrlich ascites-carcinoma cells.
1. Ehrlich ascites-cell extracts convert choline and ethanolamine approximately equally well into their respective phosphoryl derivatives. 2. Choline is a potent inhibitor of ethanolamine phosphorylation, but ethanolamine has little effect on choline phosphorylation. 3. 2,3-Dimercaptopropanol, cysteine and Ca(2+) inhibit ethanolamine phosphorylation, but have no detectable effect on choline phosphorylation. 4. Choline-phosphorylating activity in Ehrlich ascites-cell extracts is more stable during storage than ethanolamine-phosphorylating activity. 5. Choline phosphorylation is stimulated in the presence of benzoylcholine, succinylcholine, butyrylcholine and propionylcholine, whereas ethanolamine phosphorylation is inhibited. This relationship is reciprocal: the compounds causing the greatest stimulation of choline phosphorylation bring about the greatest inhibition of ethanolamine phosphorylation.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000605:Amino Alcohols / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002247:Carbon Isotopes; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002474:Cell-Free System; D002794:Choline / Q000378:metabolism* / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D003864:Depression, Chemical; D004112:Dimercaprol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000494:pharmacology; D010758:Phosphorus / Q000378:metabolism; D010759:Phosphorus Isotopes; D013268:Stimulation, Chemical
D000605:Amino Alcohols; D002247:Carbon Isotopes; D007477:Ions; D010759:Phosphorus Isotopes; D004112:Dimercaprol; D010758:Phosphorus; D000255:Adenosine Triphosphate; D003545:Cysteine; D002794:Choline; D002118:Calcium