Respiratory syncytial virus isolation by combined continuous flow-isopycnic banding centrifugation.
A new zonal centrifuge rotor (B-IX) which combines continuous sample flow centrifugation with isopycnic banding has been used to isolate and concentrate respiratory syncytial virus from liter volumes of culture fluid. This isolation technique utilizes a sucrose density gradient to trap and isopycnically band the virus particles, and permits recovery of the particles from the rotor in an unaggregated condition.
D002277:Carcinoma; D002460:Cell Line; D002501:Centrifugation, Zonal / Q000295:instrumentation*; D046508:Culture Techniques; D006801:Humans; D007822:Laryngeal Neoplasms; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D014776:Virus Cultivation