Isolation and partial characterization of nucleic acid of influenza virus.
The principal ribonucleic acid (RNA) component isolated from purified equine influenza virus has an approximate sedimentation coefficient (S(20,W)) of 21S in sucrose gradient containing 0.1 m NaCl. Three other components of 18S, 14S, and 8S were also detected. All the RNA components have characteristics of single-stranded RNA. The average base composition of the principal RNA components is cytosine, 22.2; adenine, 22.9; guanine, 22.3; and uridine, 32.6. There was no qualitative difference in the RNA isolated from noninfectious virus particles compared to that from infectious virions.
D000225:Adenine; D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002845:Chromatography; D046508:Culture Techniques; D003596:Cytosine; D005827:Genetics, Microbial; D006147:Guanine; D006387:Hemagglutination, Viral; D006389:Hemagglutinins, Viral; D006736:Horses; D009975:Orthomyxoviridae / Q000032:analysis* / Q000276:immunology; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D014316:Tritium; D014529:Uridine
D006389:Hemagglutinins, Viral; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D014529:Uridine