Regulation of choline sulphatase synthesis and activity in Aspergillus nidulans.
1. Choline O-sulphate is taken up from the growth medium to the same extent by sulphur-deficient and sulphur-sufficient mycelia of Aspergillus nidulans, but hydrolysis of the transported sulphate ester in vivo only occurs in the sulphur-deficient mycelia. 2. Choline sulphatase activity could not be detected in vitro in sulphur-sufficient mycelia of wild-type and sulphur mutants of A. nidulans, but after sulphur starvation all strains showed appreciable activity of this enzyme. 3. Optimum activity of choline sulphatase in an ultrasonically treated preparation of sulphur-deficient mycelia was at pH7.5. The optimum substrate concentration was in excess of 25mm and K(m) was 0.035m. The enzyme was completely inhibited by 10mm-SO(3) (2-), PO(4) (3-), CN(-) and cysteine. 4. Growth of sulphur-deficient mycelia on various sulphur sources resulted in a decrease of choline sulphatase activity in vitro. The decrease appeared to be due to a repression of choline sulphatase synthesis rather than to inhibition of activity. De-repression by growth on a sulphur-deficient medium was prevented by cycloheximide. Unlike the choline sulphatase of bacteria the fungal enzyme did not need to be substrate-induced. 5. By using sulphur mutants the identity of the co-repressor was limited to S(2)O(3) (2-), cysteine-S-sulphonate, cysteine or compounds derived directly from them. Circumstantial evidence suggests that the co-repressor is cysteine. 6. Inhibition of choline sulphatase activity in vivo was demonstrated with cysteine as the sulphur source for growth.
D001230:Aspergillus / Q000201:enzymology*; D002794:Choline / Q000378:metabolism; D004794:Enzyme Repression; D005827:Genetics, Microbial; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D013429:Sulfatases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D013431:Sulfates; D013455:Sulfur / Q000378:metabolism; D013460:Sulfur Isotopes; D014465:Ultrasonics
D013431:Sulfates; D013460:Sulfur Isotopes; D013455:Sulfur; D013429:Sulfatases; D002794:Choline