Isolation from yeast of a metabolically active water-soluble form of ergosterol.
A water-soluble complex containing ergosterol together with a component of yeast has been isolated. The complex can be isolated from commercial yeast extract to which ergosterol has been added or directly from whole yeast cells. The complexing component has the properties of a large polysaccharide and the binding between the sterol and the polysaccharide appears to be noncovalent. The complex is easily prepared and is stable in aqueous solution; ergosterol in this solution is metabolically available to yeast cells to which it is added.
D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004071:Digitalis Glycosides; D004121:Dimethyl Sulfoxide; D011134:Polysaccharides; D012995:Solubility; D014807:Vitamin D / Q000032:analysis*; D015002:Yeast, Dried; D015003:Yeasts
D003470:Culture Media; D004071:Digitalis Glycosides; D011134:Polysaccharides; D014807:Vitamin D; D004121:Dimethyl Sulfoxide