Selective inhibition of reovirus ribonucleic acid synthesis by cycloheximide.
Cycloheximide, at a concentration of 10 mug/ml, rapidly blocked protein synthesis in L cells infected with reovirus. When the drug was added before 5 hr postinfection, synthesis of both single- and double-stranded varieties of virus-specific ribonucleic acid (RNA), which normally commences between 6 and 7 hr after infection, was blocked. When the cycloheximide was added at 9 hr after infection, uptake of uridine-H(3) into RNA, for the succeeding 6 hr at least, was similar to that of an infected culture without the drug. This latter uptake of uridine-H(3) in the presence of cycloheximide was largely into single-stranded RNA, since double-stranded RNA synthesis was rapidly and markedly inhibited by the cycloheximide. Single-stranded RNA formed in the presence of cycloheximide was found not to be a precursor of viral progeny, double-stranded RNA. Synthesis of double-stranded RNA in the infected cell probably requires prior synthesis of a new protein, which has a rapid rate of turnover. The possibility that formation of single-stranded RNA is preceded by synthesis of a second new protein is discussed.
D000818:Animals; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D007739:L Cells; D012367:RNA, Viral / Q000096:biosynthesis*; D012087:Reoviridae / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium; D014529:Uridine; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation
D002247:Carbon Isotopes; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D003609:Dactinomycin; D003513:Cycloheximide; D014529:Uridine