Histone acetylation in insect chromosomes.
Acetylation of histones takes place along the salivary gland chromosomes of Chironomus thummi when RNA synthesis is active. It can be observed but not measured quantitatively by autoradiography of chromosome squashes. The "fixatives" commonly used in preparing squashes of insect chromosomes preferentially extract the highly acetylated "arginine-rich" histone fractions; the use of such fixatives may explain the reported absence of histone acetylation in Drosophila melanogaster.
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D001120:Arginine; D001345:Autoradiography; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D002875:Chromosomes / Q000378:metabolism*; D004175:Diptera / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006851:Hydrochloric Acid; D008099:Liver / Q000166:cytology; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D012469:Salivary Glands / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000085:Acetates; D006657:Histones; D014316:Tritium; D012313:RNA; D001120:Arginine; D006851:Hydrochloric Acid; D014529:Uridine