Heat-stable chemically defined medium for growth of animal cells in suspension.
Procedures are described for preparation of a chemically defined medium, capable of being autoclaved, for the cultivation of animal cells in suspension. Yields of 34.1 x 10(5), 33.8 x 10(5), and 47.1 x 10(5) cells per ml were recorded for cat kidney, HeLa, and mouse fibroblast (L) cells after 6, 10, and 10 days of incubation, respectively.
D003470:Culture Media; D046508:Culture Techniques; D006367:HeLa Cells / Q000254:growth & development; D006358:Hot Temperature; D007739:L Cells
D003470:Culture Media