N-methyl groups in bacterial lipids. 3. Phospholipids of hyphomicrobia.
The phospholipids of Hyphomicrobium vulgare NQ-521 have been separated by preparative thin-layer chromatography and analyzed by paper chromotography of the water-soluble products of acid and mild alkaline hydrolysis. The principal phospholipids are phosphatidyl ethanolamine (23%), phosphatidyl N,N'-dimethylethanolamine (36%), lecithin (29%), and phosphatidyl glycerol (10%). Three other strains of Hyphomicrobium were found to have similar phospholipid compositions. Growing cells incorporated the methyl group of methionine into lipid-bound N,N'-dimethylethanolamine and choline. Experiments with sonic extracts of H. vulgare NQ-521 and (14)C (methyl) S-adenosylmethionine demonstrated the formation of phosphatidyl N-monomethylethanolamine in addition to the dimethylethanolamine and choline phosphatides.
D001419:Bacteria / Q000032:analysis*; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D008055:Lipids / Q000032:analysis; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*
D002247:Carbon Isotopes; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D008715:Methionine