Hydrogen-oxidizing methane bacteria. I. Cultivation and methanogenesis.
A method for the mass culture of hydrogen-oxidizing methane bacteria has been developed; yields of 50 to 60 g (wet weight) of cells per 12 to 14 liters of culture medium were obtained. The methanogenic organism from the culture of Methanobacillus omelianskii was grown in a complex medium which was aerated with a gas mixture of hydrogen and carbon dioxide. Extracts prepared from hydrogen-grown cells formed methane from methyl cobalamin, 5-methyl tetrahydrofolate, serine, pyruvate, and carbon dioxide; these substrates have been shown to be precursors of methane in extracts of the ethyl alcohol-grown culture of M. omelianskii.
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001432:Bacteriology / Q000295:instrumentation; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D006859:Hydrogen / Q000378:metabolism* / Q000494:pharmacology; D008697:Methane / Q000096:biosynthesis*; D010084:Oxidation-Reduction
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D006859:Hydrogen; D008697:Methane