Streptococcal sialidase. II. Kinetic and immunological studies of sialidase produced by group K streptococcus.
Kinetic and immunological studies were carried out on the sialidase produced by strain 6646, group K streptococcus (K-sialidase). The K(m) values of K-sialidase were 0.9 mm for sialyllactose and 0.17 mm for bovine submaxillary mucin. The antibody against K-sialidase was produced in rabbits immunized with this enzyme. An assay procedure for determination of the anti-K-sialidase activity in terms of reciprocal of the serum dilution corresponding to the 50% inhibition point is described. Anti-K-sialidase activity is widely distributed in human sera, but this has not yet been found to be correlated with streptococcal diseases, and no definite relationship was proved between the anti-K-sialidase titer and the anti-streptolysin O titer through this study. Anti-K-sialidase serum had no effect on Vibrio cholerae sialidase.
D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D009055:Mouth / Q000382:microbiology; D009077:Mucins / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D013291:Streptococcus / Q000201:enzymology* / Q000276:immunology; D014733:Vibrio / Q000201:enzymology
D007106:Immune Sera; D009077:Mucins; D009439:Neuraminidase; D007785:Lactose