A simple and rapid procedure for the preparation of phosphopyruvate hydratase free from phosphoglycerate phosphomutase and pyruvate kinase.
D002498:Centrifugation; D011232:Chemical Precipitation; D003080:Cold Temperature; D000431:Ethanol; D006836:Hydro-Lyases / Q000032:analysis*; D007535:Isomerases / Q000032:analysis*; D008722:Methods; D011770:Pyruvate Kinase / Q000032:analysis*; D012440:Saccharomyces / Q000201:enzymology*
D000431:Ethanol; D011770:Pyruvate Kinase; D006836:Hydro-Lyases; D007535:Isomerases