Direct quantitative gas chromatographic separation of C2-C6 fatty acids, methanol, and ethyl alcohol in aqueous microbial fermentation media.
A method is described for the direct quantitative gas chromatographic separation of C(2)-C(6) lower fatty acid homologues, methanol, and ethyl alcohol in aqueous microbial fermentation media. A hydrogen flame detector and a single-phase solid column packing, comprising beads of a polyaromatic resin (polystyrene cross-linked with divinyl benzene), were employed. Direct injections of 1 to 10 muliters of aqueous culture supernatant fluids were made. Quantitative recoveries of C(2)-C(6) acids added to culture supernatant fluids were obtained.
D000085:Acetates / Q000032:analysis; D002087:Butyrates / Q000032:analysis; D002208:Caproates / Q000032:analysis; D002849:Chromatography, Gas; D003470:Culture Media / Q000032:analysis; D000431:Ethanol / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D005285:Fermentation; D000432:Methanol / Q000032:analysis*; D008722:Methods; D011108:Polymers; D011422:Propionates / Q000032:analysis
D000085:Acetates; D002087:Butyrates; D002208:Caproates; D003470:Culture Media; D005227:Fatty Acids; D011108:Polymers; D011422:Propionates; D000431:Ethanol; D000432:Methanol