Inhibition of staphylococcal enterotoxin B formation by cell wall blocking agents and other compounds.
Enterotoxin B formation by Staphylococcus aureus S6 was inhibited by Tween 80, oleic acid, sodium deoxycholate, penicillin, d-cycloserine, or bacitracin. Toxin formation by strain 243 was sensitive to oleic acid, sodium deoxycholate, sodium lauryl sulfate, d-cycloserine, or bacitracin. The effect of d-cycloserine was reversed by d-alanine with strain 243 but not with strain S6. Neither penicillin nor bacitracin inhibited alpha-hemolysin or coagulase activity of strain S6; however, 0.118 mumoles of d-cycloserine per ml increased the alpha-hemolysin titer more than eightfold. Pigmentation of strain 243 was reduced by oleic acid, sodium deoxycholate, or methicillin, and was completely inhibited by d-cycloserine or bacitracin. Glucose was required for the inhibition by spermine of (14)C-valine incorporation into cellular protein of strain S6. These data indicate that the cell surface may contain sites important to the synthesis of enterotoxin B.
D000409:Alanine / Q000494:pharmacology; D001414:Bacitracin / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000494:pharmacology; D002247:Carbon Isotopes; D002473:Cell Wall / Q000187:drug effects*; D003030:Coagulase / Q000096:biosynthesis; D003523:Cycloserine / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004768:Enterotoxins / Q000096:biosynthesis*; D006460:Hemolysin Proteins / Q000096:biosynthesis; D009829:Oleic Acids / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D010860:Pigments, Biological / Q000378:metabolism; D013096:Spermine / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects* / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D014633:Valine / Q000378:metabolism
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D003030:Coagulase; D003902:Detergents; D004768:Enterotoxins; D006460:Hemolysin Proteins; D009829:Oleic Acids; D010406:Penicillins; D010860:Pigments, Biological; D013501:Surface-Active Agents; D001414:Bacitracin; D013096:Spermine; D003523:Cycloserine; D014633:Valine; D000409:Alanine