Identification of malic acid from yeast.
Evidence indicated an accumulation of l-malic acid in the synthetic growth medium of both lysine auxotrophs and wild-type yeast. Malic acid was isolated from growth experiments and was identified by paper and column chromatography, by comparison of infrared spectra with authentic l-malic acid, as well as enzymatically, as a substrate of malic dehydrogenase. Malic acid was accumulated when glucose was used as a carbon source. This did not occur when sodium lactate was the carbon source. Malic, succinic, and citric acids, and a few other as yet unidentified organic acids, were accumulated in the culture supernatant fluid when yeast was grown in a chemically defined medium. The accumulation of these acids had no obvious relation to the pathway for lysine biosynthesis.
D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D002845:Chromatography; D002854:Chromatography, Paper; D002951:Citrates / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008293:Malates / Q000032:analysis*; D009154:Mutation; D012440:Saccharomyces / Q000032:analysis* / Q000378:metabolism; D013386:Succinates / Q000032:analysis
D000085:Acetates; D002247:Carbon Isotopes; D002951:Citrates; D007773:Lactates; D008293:Malates; D013386:Succinates; D005947:Glucose; D008239:Lysine