Protein synthesis in the hippocampal pyramidal cells of rats during a behavioral test.
Protein synthesis was studied in the pyramidal nerve cells of the CA3 region in the hippocampus of rats uring a behavioral test involving transfer of handedness. A new electrophoretic technique was used for separation of 10(-7) to 10(-9) gram of protein and radiometric determination of the various protein fractions. After interventricular administration of (3)H-leucine, protein synthesis of two fast-moving fractions was significantly higher bilaterally in the hippocampus of the trained rats. There was also a trend to lateralization of the highest protein synthesis to the learning side.
D000180:Acrylic Resins; D000818:Animals; D001522:Behavior, Animal; D004586:Electrophoresis; D007839:Functional Laterality; D006624:Hippocampus / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D014163:Transfer, Psychology; D014316:Tritium
D000180:Acrylic Resins; D009419:Nerve Tissue Proteins; D014316:Tritium; D007930:Leucine