Different lethal effects of mitomycin C and actinomycin D during the division cycle of HeLa cells.
The lethal actions of mitomycin C and actinomycin D were followed during the division cycle of HeLa cells. The cells were most susceptible to a 2 hr pulse of mitomycin C during the G(1) phase, whereas their sensitivity to actinomycin D was most pronounced in the S phase. Posttreatment of the cells with acetoxycycloheximide, a potent inhibitor of protein synthesis, increased the survival (colony-forming ability) of cells treated with mitomycin C but had very little effect on the survival of cells treated with actinomycin D. The significance of these findings is discussed.
D002455:Cell Division / Q000187:drug effects*; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D008937:Mitomycins / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis
D004273:DNA, Neoplasm; D008937:Mitomycins; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D003609:Dactinomycin; D003513:Cycloheximide