Enzymes of the mandelate pathway in Bacterium N.C.I.B. 8250.
1. Bacterium N.C.I.B. 8250 was grown on dl-mandelate, benzyl alcohol, benzoyl-formate, benzaldehyde and benzoate and also on 2-hydroxy, 4-hydroxy, 3,4-dihydroxy and 4-hydroxy-3-methoxy analogues of these compounds. The enzymic complements of the cells were determined and the specificities of some of the enzymes examined. 2. Growth on mandelate or benzoylformate induces l-mandelate dehydrogenase, benzoylformate decarboxylase, benzyl alcohol dehydrogenase and a heat-stable as well as a heat-labile benzaldehyde dehydrogenase. Growth on benzyl alcohol or benzaldehyde induces benzyl alcohol dehydrogenase and the heat-labile benzaldehyde dehydrogenase. 3. The enzymes of the mandelate-to-benzoate pathway are non-specifically active on, and induced by, all the substituted analogues that support growth. 4. Benzoate oxidase is induced by growth on benzoate or on 2-hydroxybenzoate. 2-Hydroxybenzoate hydroxylase, 4-hydroxybenzoate hydroxylase and 4-hydroxy-3-methoxybenzoate O-demethylase are induced only by growth on homologous substrates. 5. The results of the investigation are discussed with regard to the possible regulation of the enzyme systems.
D000447:Aldehydes / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D002262:Carboxy-Lyases; D004790:Enzyme Induction; D005561:Formates / Q000378:metabolism; D008333:Mandelic Acids / Q000378:metabolism*; D010088:Oxidoreductases; D010636:Phenols / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D014733:Vibrio / Q000201:enzymology*
D000447:Aldehydes; D001565:Benzoates; D005561:Formates; D008333:Mandelic Acids; D010636:Phenols; D013386:Succinates; D010088:Oxidoreductases; D002262:Carboxy-Lyases