Relationship of germinal centers in lymphoid tissue to immunological memory. II. The detection of primed cells and their proliferation upon cell transfer to lethally irradiated syngeneic mice.
White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing. White pulp was a much richer source of responsive cells than was whole spleen.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001345:Autoradiography; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D007112:Immunity, Maternally-Acquired; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D011830:Radiation Effects; D012756:Sheep; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D014316:Tritium; D001973:Bromodeoxyuridine; D014529:Uridine