Preservation of influenza virus infectivity by lyophilization.
A method of lyophilizing influenza virus in allantoic fluid with retention of high-titer of egg infectivity is described. Five strains of virus were lyophilized, and all were much more stable than fluid virus preparations, retaining 2 to 3 logs of infectivity after storage at 37 C for 60 to 95 days. Statistical analysis of an accelerated storage test by extrapolation of viral degradation indicates that the lyophilized viruses are stable indefinitely at or below room temperature.
D000818:Animals; D002642:Chick Embryo; D005612:Freeze Drying; D009975:Orthomyxoviridae / Q000472:pathogenicity; D013696:Temperature