Some effects of ouabain and potassium on transport and metabolism in rat small intestine.
1. Ouabain (10(-3)M) inhibited the transfer of glucose, proline, methionine and fluid but not of galactose and 3-O-methyl glucose by sacs of rat everted intestine.2. Ouabain (10(-3)M) inhibited both glucose and mannose metabolism. Hexose metabolism was also reduced by varying the [K] from 4.8 m-equiv/l.3. Ouabain (10(-3)M) or absence of K reduced the stimulation of amino acid, hexose and fluid transfer by mannose. Although presence of ouabain or absence of K did not inhibit endogenous galactose transfer, these conditions prevented mannose from stimulating this transfer.4. Ouabain (10(-3)M) exerted greater effects on hexose and amino acid transfer when K was omitted from the saline and its inhibitory effects were partially overcome by increased serosal [K].5. Ouabain (10(-2)M) caused further inhibition of amino acid and glucose transfer and also reduced the transfer of galactose and 3-O-methyl glucose.6. The implications of these results are discussed with respect to the relationships between cations and hexose and amino acid transfer.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects*; D005110:Extracellular Space; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D007583:Jejunum; D008297:Male; D008358:Mannose / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D051381:Rats
D000596:Amino Acids; D006601:Hexoses; D010042:Ouabain; D011392:Proline; D008715:Methionine; D005947:Glucose; D008358:Mannose; D011188:Potassium; D005690:Galactose