The glycolipids of Lactobacillus casei A.T.C.C. 7469.
1. The lipids were extracted from Lactobacillus casei A.T.C.C. 7469 with chloroform-methanol mixtures. The glycolipids were obtained by chromatography on silicic acid and DEAE-cellulose (acetate form). 2. Hydrolysis of the glycolipids with alkali gave two glycerol glycosides and a mixture of fatty acids. 3. The glycosides were separated and their structures elucidated. The major component was O-alpha-d-galactopyranosyl-(1-->2)-O-alpha-d-glucopyranosyl-(1-->1)-glycerol and the minor component O-alpha-d-glucopyranosyl-(1-->6)-O-alpha-d-galactopyranosyl-(1-->2)-O-alpha-d-glucopyranosyl-(1-->1)-glycerol. 4. Analysis of the fatty acids by gas-liquid chromatography showed that they were predominantly palmitic acid, octadecenoic acid and lactobacillic acid.
D002725:Chloroform; D002845:Chromatography; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis; D007778:Lactobacillus / Q000032:analysis*; D000432:Methanol; D010169:Palmitic Acids / Q000032:analysis
D005227:Fatty Acids; D006017:Glycolipids; D006027:Glycosides; D010169:Palmitic Acids; D002725:Chloroform; D000432:Methanol