Actinomycin D: inhibition of phospholipid synthesis in chick embryo cells.
When chick embryo fibroblasts are exposed to actinomycin D for 30 minutes to 3 hours, there is progressive inhibition of phospholipid synthesis, so that by 3 hours the inhibition is 40 percent. All phospholipids are affected. Since this inhibition is twice as great as the inhibition of protein synthesis, some effects of actinomycin D previously ascribed to decreased protein synthesis must be reevaluated.
D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D003609:Dactinomycin / Q000494:pharmacology*; D003864:Depression, Chemical; D005347:Fibroblasts / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D010759:Phosphorus Isotopes; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D010743:Phospholipids; D010759:Phosphorus Isotopes; D014316:Tritium; D003609:Dactinomycin; D014498:Uracil; D012313:RNA; D007930:Leucine