Metabolism of phospholipids and the characterization of fatty acids in bovine corpus luteum.
1. Phosphatidylcholine was the predominant phospholipid in bovine corpora lutea; it accounted for about 50% of the total phospholipid phosphorus. Phosphatidylethanolamine (13%) and ethanolamine plasmalogen (8-9%) were the next two major components. 2. After incubation of the tissue with [(32)P]orthophosphate the total radioactivity and specific radioactivity of phosphatidylinositol were higher than those of any other lipid. 3. Luteinizing hormone failed to increase significantly the incorporation of [(32)P]orthophosphate into total phospholipids from luteal tissue slices, but did stimulate progesterone synthesis and lactate production. 4. The proportion of oleate (18:1) in the neutral lipids and phospholipids was higher than that of any other fatty acid. 5. The proportion of unsaturated fatty acid in the tissue lipids exceeded 60%, and almost half of this was polyunsaturated. Arachidonate (20:4), docosatetraenoate (22:4) and docosapentaenoate (22:5) were the principal polyunsaturated fatty acids. 6. After incubation of luteal tissue with [1-(14)C]acetate, the greatest proportion of radioactivity in the fatty acids isolated from the total lipid fraction was in palmitate (16:0) and docosatetraenoate (22:4). Polyunsaturated fatty acids accounted for almost 50% of the (14)C radioactivity incorporated and this pattern was observed in phospholipids, triglycerides and free fatty acids.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D003338:Corpus Luteum / Q000032:analysis / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005260:Female; D007986:Luteinizing Hormone; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D010759:Phosphorus Isotopes; D010955:Plasmalogens / Q000378:metabolism*
D000085:Acetates; D002247:Carbon Isotopes; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010759:Phosphorus Isotopes; D010955:Plasmalogens; D007986:Luteinizing Hormone