Molluscan cells: dissociation and reaggregation.
Dissociation of the ovotestes of Helix pomatia requires the presence of carbohydrates as well as trypsin, but is independent of cation content. Dispersed cells obtained from the ovotestes reaggregate rapidly into histotypically organized tissue. The role of cellular adhesiveness is facilitated by centrifugation, but pseudopodial activity of the cells is suggested as another factor in reaggregation.
D000818:Animals; D002449:Cell Aggregation; D002498:Centrifugation; D003470:Culture Media; D004187:Disaccharides; D005690:Galactose; D005942:Gluconates; D006066:Gonads / Q000166:cytology*; D066298:In Vitro Techniques; D012908:Snails / Q000166:cytology*; D012965:Sodium Chloride; D014357:Trypsin
D003470:Culture Media; D004187:Disaccharides; D005942:Gluconates; D012965:Sodium Chloride; D014357:Trypsin; D005690:Galactose