Two forms of aspartate aminotransferase in rat liver and kidney mitochondria.
1. Butan-1-ol solubilizes that portion of rat liver mitochondrial aspartate aminotransferase (EC 2.6.1.1) that cannot be solubilized by ultrasonics and other treatments. 2. A difference in electrophoretic mobilities, chromatographic behaviour and solubility characteristics between the enzymes solubilized by ultrasonic treatment and by butan-1-ol was observed, suggesting the occurrence of two forms of this enzyme in rat liver mitochondria. 3. Half the aspartate aminotransferase activity of rat kidney homogenate was present in a high-speed supernatant fraction, the remainder being in the mitochondria. 4. A considerable increase in aspartate aminotransferase activity was observed when kidney mitochondrial suspensions were treated with ultrasonics or detergents. 5. All the activity after maximum activation was recoverable in the supernatant after centrifugation at 105000g for 1hr. 6. The electrophoretic mobility of the kidney mitochondrial enzyme was cathodic and that of the supernatant enzyme anodic. 7. Cortisone administration increased the activities of both mitochondrial and supernatant aspartate aminotransferases of liver, but only that of the supernatant enzyme of kidney.
D000438:Alcohols; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D003348:Cortisone / Q000494:pharmacology; D003902:Detergents; D004586:Electrophoresis; D007668:Kidney / Q000166:cytology / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D012995:Solubility; D013268:Stimulation, Chemical; D014465:Ultrasonics
D000438:Alcohols; D003902:Detergents; D001219:Aspartate Aminotransferases; D003348:Cortisone