Heat inactivation of enterotoxin A from Staphylococcus aureus in veronal buffer.
Serological tests were used to determine the slope of the thermal inactivation curve of crude enterotoxin A in Veronal buffer (pH 7.2), and the resulting z value was 27.8 C. (50 F). Serological assays also showed that the heat inactivation at each time-temperature depended on the original concentration of enterotoxin A. The usefulness of the Oudin tube serological test for determining end points of inactivation of naturally produced enterotoxin A (not concentrated) is discussed. We concluded that this test cannot be used to determine end points of heat inactivation for enterotoxin A in the minute quantities naturally produced in foods.
D002021:Buffers; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis; D005516:Food Microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D011233:Precipitin Tests; D013210:Staphylococcus / Q000378:metabolism*
D002021:Buffers; D004768:Enterotoxins