Liquid nitrogen preservation of standard inoculum: gas-phase storage.
Liquid nitrogen storage was the most satisfactory of several methods tested for supplying standard Streptomyces viridoflavus inoculum for laboratory and pilot plant experimentation. Shake-flask cultures were subdivided into sterile cotton-plugged ampoules and stored in the gas space of a liquid nitrogen refrigerator. There were no detectable changes in viability or in candidin-producing capacity over a 12-month test period. The procedure also proved satisfactory with all other organisms tested.
D005615:Freezing; D009584:Nitrogen; D011309:Preservation, Biological; D013302:Streptomyces / Q000254:growth & development
D009584:Nitrogen