A study of urinary excretion of parathyroid hormone in man.
A procedure for bioassaying parathyroid hormone-like activity in human urine has been developed. 24-hr urine samples were concentrated with dry Sephadex G-25 and bioassayed in the young thyroparathyrocauterized mouse by the measurement of whole blood calcium. Recovery of biological activity and radioiodinated beef parathyroid hormone was over 80%. Normal subjects usually excreted less than 30 U (USP) of activity per day while 18 patients with proven primary hyperparathyroidism excreted a mean of 182 U/day (USP). The activity was not found in 7 patients with hypoparathyroidism or in 5 patients with carcinoma of the breast, but was present in 9 patients with uremia and in 5 with carcinoma of the lung and hypercalcemia.
D000818:Animals; D001681:Biological Assay; D001943:Breast Neoplasms / Q000652:urine; D002118:Calcium / Q000097:blood; D006801:Humans; D006934:Hypercalcemia / Q000652:urine; D006961:Hyperparathyroidism / Q000652:urine; D007011:Hypoparathyroidism / Q000652:urine; D007456:Iodine Isotopes; D007674:Kidney Diseases / Q000652:urine; D008175:Lung Neoplasms / Q000652:urine; D008722:Methods; D051379:Mice; D010281:Parathyroid Hormone / Q000652:urine*; D014020:Tissue Extracts / Q000652:urine; D014511:Uremia / Q000652:urine
D007456:Iodine Isotopes; D010281:Parathyroid Hormone; D014020:Tissue Extracts; D002118:Calcium