The tissue content and turnover rates of intermediates in the biosynthesis of glycosaminoglycans in young rat skin.
1. The tissue contents of hexose monophosphate, N-acetylglucosamine 6-phosphate, UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine and UDP-glucuronic acid were determined in the skin of young rats less than 1 day post partum. Tissue-space determinations were used to calculate their average cellular concentrations. 2. The incorporation of [U-(14)C]-glucose into the intermediates was recorded with time and their rates of turnover were calculated. The results demonstrated product-precursor relationships along the pathway of hexosamine synthesis and that of hexuronic acid synthesis. The rates of synthesis of UDP-N-acetylhexosamine and UDP-glucuronic acid were 1.5+/-0.3 and 0.24+/-0.03mmumoles/min./g. of tissue respectively. These results indicated the average turnover time of the total tissue glycosaminoglycans to be about 5 days.
D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D002247:Carbon Isotopes; D002498:Centrifugation; D002852:Chromatography, Ion Exchange; D005110:Extracellular Space / Q000032:analysis; D005690:Galactose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism*; D006600:Hexosephosphates / Q000378:metabolism*; D008954:Models, Biological; D011134:Polysaccharides / Q000096:biosynthesis*; D051381:Rats; D012867:Skin / Q000378:metabolism*; D013997:Time Factors; D014500:Uracil Nucleotides / Q000378:metabolism*
D001786:Blood Glucose; D002247:Carbon Isotopes; D005965:Glucuronates; D006600:Hexosephosphates; D011134:Polysaccharides; D014500:Uracil Nucleotides; D005947:Glucose; D005944:Glucosamine; D005690:Galactose