Induction of bone marrow colony-stimulating activity by a filterable agent in leukemic and normal mouse serum.
1. Leukemic Swiss mice of ICR/Ha strain which had been injected at birth with a lymphoid-leukemia-inducing virus preparation yielded sera which produced elevations of serum colony-stimulating activity within 16 hr and significant plasma-LDH-enzyme elevation at 4 days when injected intraperitoneally into normal ICR/Ha Swiss mice. Colony-stimulating activity was assayed in vitro by the stimulation of hemopoietic colony formation by DBA/1 bone marrow cells. 2. The inducing agent in leukemic serum was passageable, filterable, sedimentable, and heat-, ether-, and UV-labile. 3. A similar agent was recovered from normal Swiss serum after blind serial passages through normal mice. 4. LDH elevating virus induced a similar elevation of serum colony-stimulating activity when injected at high titers, and cross-resistance was demonstrated between LDH virus and the passaged leukemic serum agent.
D000818:Animals; D001853:Bone Marrow / Q000254:growth & development* / Q000276:immunology; D001854:Bone Marrow Cells; D046508:Culture Techniques; D006410:Hematopoiesis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007942:Leukemia, Experimental / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D011817:Rabbits; D012190:Retroviridae
D007770:L-Lactate Dehydrogenase