Mass preparation of nuclei from the larval salivary glands of Drosophila hydei.
A method has been developed for isolating gram quantities of salivary glands from late third instar larvae of Drosophila hydei. The isolated glands have a normal appearance and incorporate RNA and DNA precursors normally. Nuclei can be isolated from these glands in 90% yield with the use of detergents. These nuclei contain morphologically normal giant polytene chromosomes.
D000818:Animals; D002467:Cell Nucleus; D002875:Chromosomes; D004247:DNA / Q000032:analysis; D004330:Drosophila / Q000166:cytology*; D008722:Methods; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D012469:Salivary Glands / Q000032:analysis / Q000166:cytology*
D011506:Proteins; D012313:RNA; D004247:DNA