Centrifugation of mammalian cells on gradients: a new rotor.
A short-arm rotor increases separation of viable mammalian cells, from mixtures, by low-speed centrifugation; continuous Ficoll density gradients in tissue-culture media are used. We describe the theory and experimental demonstration of the superior separation achieved with this new rotor.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D003585:Cell Biology / Q000295:instrumentation*; D002499:Centrifugation, Density Gradient / Q000295:instrumentation*; D003470:Culture Media; D006367:HeLa Cells; D008322:Mammals; D008722:Methods; D011134:Polysaccharides; D012399:Rotation
D003470:Culture Media; D011134:Polysaccharides