The determination of tocopherols and isoprenoid quinones in the grain and seedlings of wheat (Triticum vulgare).
1. A comparison is made of several procedures for the extraction of tocopherols and isoprenoid quinones from plant tissues. 2. Gradient-elution column chromatography on acid-washed alumina efficiently separates the isoprenoid quinones and tocopherols into groups that can then be assayed spectrophotometrically or, with the tocopherols, separated into their individual components and determined by gas-liquid chromatography. 3. This improved analytical procedure was used to study the distribution of the tocopherols and of ubiquinone in the ungerminated wheat grain.
D000433:1-Propanol; D002725:Chloroform; D002845:Chromatography; D002849:Chromatography, Gas; D008722:Methods; D010936:Plant Extracts / Q000032:analysis; D011809:Quinones / Q000032:analysis*; D013053:Spectrophotometry; D014908:Triticum / Q000032:analysis* / Q000196:embryology; D014451:Ubiquinone / Q000032:analysis; D014810:Vitamin E / Q000032:analysis*
D010936:Plant Extracts; D011809:Quinones; D014451:Ubiquinone; D014810:Vitamin E; D002725:Chloroform; D000433:1-Propanol