Breakdown and assembly of polyribosomes in synchronized Chinese hamster cells.
Polyribosomes were absent in metaphase Chinese hamster cells and were reassembled after mitosis. This assembly correlated temporally with formation of nuclear membrane and decondensation of chromatin, accounted for a threefold increased rate of protein synthesis, and occurred independently of de novo RNA synthesis, that is, it utilized preexisting messenger RNA.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D046508:Culture Techniques; D003609:Dactinomycin / Q000494:pharmacology; D003864:Depression, Chemical; D008938:Mitosis; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D012333:RNA, Messenger; D014316:Tritium; D003609:Dactinomycin; D012313:RNA; D014529:Uridine