Specific distribution of short-chain fatty acids in molecular distillates of bovine milk fat.
Triglycerides with a total of 34-44 acyl carbon atoms have previously been shown to account for nearly 50% of bovine milk fat. About 80% of this material was isolated by molecular distillation and a representative fraction of these glycerides was subjected to a stereospecific analysis. Of the 29 moles % of C(4)-C(8) fatty acids present in the analyzed fraction, at least 95% were specifically attached to the glycerol molecule in the position corresponding to carbon 3 of sn-glycerol 3-(dihydrogen phosphate) (L-alpha-glycerophosphate). The distribution of the other fatty acids (C(10) or greater) did not show such marked specificity for either the 1- or the 2-position. The data support the hypothesis that the short-chain fatty acids are esterified with long-chain diglycerides, or are substituted in glycerophosphatide intermediates, during the final step in the biosynthesis of milk fat triglycerides.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D005223:Fats / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005989:Glycerides / Q000032:analysis; D005990:Glycerol; D005994:Glycerophosphates; D008049:Lipase; D008892:Milk / Q000032:analysis*; D010636:Phenols / Q000032:analysis; D010740:Phospholipases; D014280:Triglycerides / Q000032:analysis*
D005223:Fats; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D005994:Glycerophosphates; D010636:Phenols; D014280:Triglycerides; D010740:Phospholipases; D008049:Lipase; D005990:Glycerol