Density gradient centrifugation: fixation of bands by photopolymerization of acrylamide.
A method for immobilization of proteins in a density gradient is described. This method eliminates the collection of drops or the use of a schlieren optical system, but visualizes the results of the Amido Schwarz stain. Although only sucrose gradients have been examined, the method possibly may be extended to other gradients.
D000180:Acrylic Resins; D000429:Alcohol Oxidoreductases / Q000032:analysis; D002499:Centrifugation, Density Gradient; D005782:Gels; D008722:Methods; D009113:Muramidase / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D013395:Sucrose
D000180:Acrylic Resins; D005782:Gels; D012710:Serum Albumin, Bovine; D013395:Sucrose; D000429:Alcohol Oxidoreductases; D009113:Muramidase