The partial characterization of 1-oxygenated steroids from urine of a hypertensive newborn child.
1. Four substances from the urine of a hypertensive newborn girl were partially characterized and shown to be 17alpha-hydroxy-5beta-pregnane-1,3,20-trione, 3alpha,17alpha-dihydroxy-5beta-pregnane-1,20-dione, 3alpha,17alpha,20alpha-trihydroxy-5beta-pregnan-1-one and 5beta-pregnane-1beta,3alpha,17alpha,20alpha-tetrol. 2. The characterization rested mainly on R(M) analysis of the substances and their derivatives by glycol fission, providing evidence for position and degree of substitution and for steroidal character. Supporting evidence was provided by chemically specific location reactions. 3. Certain problems in the manipulation of these beta-disubstituted steroids are discussed.
D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D005260:Female; D006801:Humans; D006973:Hypertension / Q000151:congenital* / Q000652:urine*; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000652:urine*; D011278:Pregnanes / Q000652:urine*
D011278:Pregnanes