Enzyme-catalyzed reactions of the carcinogen N-hydroxy-2-fluorenylacetamide with nucleic acid.
A protein fraction obtained by gel filtration of a 105,000g supernatant of rat liver catalyzes three reactions of the hepatocarcinogen N-hydroxy-2-fluorenylacetamide with nucleic acid. Cofactor requirements and isotopic studies suggest that the reactive intermediates involved may be N-2-fluorenylhydroxylamine, and phosphate and sulfate esters of N-hydroxy-2-fluorenylacetamide.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002247:Carbon Isotopes; D002273:Carcinogens / Q000378:metabolism*; D002850:Chromatography, Gel; D005449:Fluorenes / Q000378:metabolism*; D006898:Hydroxylamines; D008110:Liver Extracts / Q000032:analysis / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008345:Manganese / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D013431:Sulfates
D002247:Carbon Isotopes; D002273:Carcinogens; D005449:Fluorenes; D006898:Hydroxylamines; D008110:Liver Extracts; D011506:Proteins; D013431:Sulfates; D008345:Manganese; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D010770:Phosphotransferases; D008274:Magnesium