Assay for aflatoxin production by the genera Aspergillus and Penicillium.
A total of 260 isolates, including 43 species of Penicillium and 7 species of Aspergillus, were screened for their ability to produce aflatoxin on rice. Chloroform extracts were analyzed by thin-layer chromatography. None of the isolates produced aflatoxin. Certain species of Penicillium produced fluorescent substances that either were similar in R(F) or were of similar color to B and G aflatoxins. These substances were subsequently proved not to be aflatoxin by two-dimensional chromatography, by reaction with iodine fumes, or by both methods.
D000348:Aflatoxins / Q000032:analysis / Q000096:biosynthesis*; D001230:Aspergillus / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D012275:Oryza; D010407:Penicillium / Q000378:metabolism*
D000348:Aflatoxins