Hydrolysis at arginylproline in polypeptides by clostridiopeptidase B.
Clostridiopeptidase B, a sulfhydryl protease from Clostridium histolyticum, hydrolyzes the arginylproline bond in the synthetic polypeptide methionyllysyl-bradykinin. Hydrolysis also occurs at a reduced rate at the lysylarginine bond, further delineating the environment necessary for the minor proteolysis seen with this enzyme at lysine residues. The specificity of clostridiopeptidase B differs from trypsin, which hydrolyzes this synthetic polypeptide only at the lysylarginine bond.
D000595:Amino Acid Sequence; D001120:Arginine; D055598:Chemical Phenomena; D002621:Chemistry; D003013:Clostridium; D004586:Electrophoresis; D010450:Endopeptidases; D008239:Lysine; D010455:Peptides / Q000032:analysis; D011392:Proline; D014357:Trypsin
D010455:Peptides; D001120:Arginine; D011392:Proline; D010450:Endopeptidases; D014357:Trypsin; D008239:Lysine