High-resolution nuclear magnetic resonance spectra of selectively deuterated staphylococcal nuclease.
An analog of staphylococcal nuclease has been prepared in which all amino acids, except the six following, are fully deuterated: tryptophan; methionine; tyrosine, in ring positions 2 and 6; histidine, in ring position 2; aspartic acid and asparagine, beta-methylene; and glutamic acid and glutamine, gamma-methylene. The analog has a much simpler high-resolution nuclear magnetic resonance spectrum than the fully protonated enzyme. The effects of calcium ion and of the inhibitor 3', 5'-thymidine diphosphate on the spectrum of the analog were readily detected.
D000596:Amino Acids / Q000032:analysis*; D001216:Asparagine; D001224:Aspartic Acid; D002118:Calcium; D003851:Deoxyribonucleases / Q000032:analysis*; D003903:Deuterium; D005971:Glutamates; D005973:Glutamine; D006639:Histidine; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine; D009711:Nucleotides; D012260:Ribonucleases / Q000032:analysis*; D013210:Staphylococcus; D014364:Tryptophan; D014443:Tyrosine
D000596:Amino Acids; D005971:Glutamates; D009711:Nucleotides; D005973:Glutamine; D001224:Aspartic Acid; D014443:Tyrosine; D006639:Histidine; D001216:Asparagine; D014364:Tryptophan; D008715:Methionine; D003903:Deuterium; D003851:Deoxyribonucleases; D012260:Ribonucleases; D002118:Calcium