The complexing of lysozyme with poly C and other homopolymers.
Lysozyme forms very large complexes with poly C, in acetate buffer solutions (pH 5.4), when the ratio of lysozyme to poly C concentration is 3/2. When this is less than 3/8 there is virtually no complexing, as evidenced by the low light-scattering power of such mixtures. At such relatively high poly C concentrations, the addition of pancreatic ribonuclease causes both the intensity and dissymmetry of scattering to rise to very high values after which time the intensity falls exponentially with time and with very little change in dissymmetry. Other homopolymers also form largest complexes with lysozyme at characteristic concentration ratios.
D000227:Adenine Nucleotides; D055598:Chemical Phenomena; D002621:Chemistry; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009113:Muramidase; D010179:Pancreas / Q000201:enzymology; D011119:Polynucleotides; D012260:Ribonucleases; D013053:Spectrophotometry; D013997:Time Factors; D014500:Uracil Nucleotides
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D011119:Polynucleotides; D014500:Uracil Nucleotides; D012260:Ribonucleases; D009113:Muramidase