The effect of light and inhibitors on chloroplast and cytoplasmic RNA synthesis.
Chloroplast RNA is synthesized in dark-grown radish cotyledons at about one-third the rate of that in the light. The synthesis, however, continues for longer in the dark and the percentage of chloroplast RNA can approach that in light-grown tissue. Light stimulates the synthesis and accumulation of both cytoplasmic and chloroplast RNA, but shows a 4-fold greater stimulation of the chloroplast RNA. Chloramphenicol, streptomycin and cycloheximide inhibit the synthesis of chloroplast RNA with little effect on cytoplasmic RNA. 5-Fluorouracil inhibits the synthesis of cytoplasmic more than chloroplast RNA. Synthesis of the 0.56 x 10(6) mol wt chloroplast RNA is inhibited much less than the other ribosomal RNA components by actinomycin D.
D000963:Antimetabolites / Q000494:pharmacology; D002736:Chloroplasts / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D008027:Light; D010944:Plants; D012313:RNA / Q000096:biosynthesis*; D013997:Time Factors
D000963:Antimetabolites; D012313:RNA