Ribonuclease activity in commercial crystalline trypsin and a method for removal.
Several preparations of crystalline trypsin hydrolyze RNA because of contaminating ribonuclease activity. Filtration of these materials through Sephadex G-50 yields a trypsin devoid of ribonuclease activity and having a proteolytic specific activity about 70 percent of the starting material.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002850:Chromatography, Gel; D003460:Crystallization; D008722:Methods; D012334:RNA, Neoplasm; D012260:Ribonucleases / Q000032:analysis*; D012995:Solubility; D014357:Trypsin / Q000302:isolation & purification*
D012334:RNA, Neoplasm; D012260:Ribonucleases; D014357:Trypsin