The preparation and properties of pyruvate kinase attached to porous sheets, and the operation of a two-enzyme continuous-feed reactor.
1. The covalent attachment of pyruvate kinase to filter-paper disks and some kinetic properties of the resultant enzymically active porous sheets are described. 2. A two-enzyme reactor was constructed by using water-insoluble sheets of lactate dehydrogenease in conjunction with the above-mentioned sheets possessing pyruvate kinase activity.
D000227:Adenine Nucleotides; D000327:Adsorption; D002482:Cellulose; D002616:Chemical Engineering / Q000295:instrumentation*; D004058:Diffusion; D005374:Filtration; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase; D010209:Paper; D010539:Permeability; D011770:Pyruvate Kinase
D000227:Adenine Nucleotides; D007527:Isoenzymes; D002482:Cellulose; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase