Respiratory pigments of Crithidia fasciculata.
Mitochondria were isolated from the heme-requiring insect trypanosomatid, Crithidia fasciculata, which had respiratory activity, showed a P/O ratio with succinate of 0.5 to 1.0, and contained 40 to 50% of the heme a and heme c found in the intact cells. Cytochromes b, c(555), possibly c(1), cytochrome oxidase, a carbon monoxide-binding pigment, and flavoproteins were detectable in the spectra of both intact cells and mitochondria. Cytochrome c(555) is a basic protein that was extracted from cells and mitochondria with salt solutions. The molar ratio of heme c to heme a was approximately 2:1 in both cells and mitochondria. This organism could possibly serve as a model for studies of the respiratory activity of the pathogenic trypanosomes.
D002474:Cell-Free System; D003580:Cytochromes / Q000378:metabolism*; D056890:Eukaryota / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011048:Polarography; D013053:Spectrophotometry
D003580:Cytochromes; D006418:Heme