Pyrimidine biosynthesis in Lactobacillus leichmannii.
Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the carbon dioxide pool utilized for purine biosynthesis. The enzyme of the first reaction leading from arginine to pyrimidines, arginine deiminase, was investigated in crude bacterial extracts. It was inhibited by thymidylic acid and purine ribonucleotides, and to a lesser extent by purine deoxynucleotides and deoxycytidylic acid. Under the assay conditions employed, a number of nucleotides had no effect on the enzyme activity of the aspartate transcarbamylase of L. leichmannii. Growth of the cells in media containing uracil, compared to growth in media without uracil, resulted in a four- to fivefold decrease in the concentrations of aspartate transcar-bamylase and dihydroorotase and a twofold increase in the concentration of arginine deiminase, as estimated from specific enzyme activity in crude extracts of the cells. A small increase in specific enzyme activity of ornithine transcarbamylase and carbamate kinase was also observed in extracts obtained from cells grown on uracil. No appreciable change in concentration of any of the five enzymes studied was detected when the cells were grown in media containing thymidine or guanylic acid. A hypothetical scheme which suggests a relationship between the control of purine and pyrimidine biosynthesis in this bacterium and which is consistent with the experimental results obtained is presented.
D001120:Arginine / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D006150:Guanine Nucleotides / Q000494:pharmacology; D006867:Hydrolases / Q000037:antagonists & inhibitors; D007778:Lactobacillus / Q000378:metabolism*; D009711:Nucleotides / Q000494:pharmacology; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011743:Pyrimidines / Q000096:biosynthesis*; D013936:Thymidine / Q000494:pharmacology; D014166:Transferases / Q000378:metabolism; D014498:Uracil / Q000494:pharmacology
D002247:Carbon Isotopes; D006150:Guanine Nucleotides; D009711:Nucleotides; D011743:Pyrimidines; D002245:Carbon Dioxide; D001224:Aspartic Acid; D014498:Uracil; D001120:Arginine; D014166:Transferases; D009954:Ornithine Carbamoyltransferase; D010770:Phosphotransferases; D006867:Hydrolases; D013936:Thymidine