Choline acetyltransferase binding to and release from membranes.
1. The binding of non-occluded choline acetyltransferase to synaptosome membranes is a reversible process that is primarily dependent on the pH and ionic strength of the suspending medium. 2. The distribution of soluble enzyme bound to synaptosome membranes was studied by density-gradient centrifuging. 3. Choline acetyltransferase shows enzyme activity both in the free and in the membrane-bound form. 4. Varying the temperature or prolonged hypo-osmotic treatment does not release the membrane-bound enzyme. 5. The release of choline acetyltransferase from membranes by different anions, thiols, adenosine nucleotides and enzyme substrates was studied.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions; D008566:Membranes; D051381:Rats; D013569:Synapses / Q000201:enzymology*; D013997:Time Factors
D007038:Hypotonic Solutions; D000217:Acyltransferases