The purification and some properties of a beta-lactamase (cephalosporinase) synthesized by Enterobactercloacae.
1. A beta-lactamase has been purified from a strain of Enterobacter cloacae. 2. This enzyme is about eighty times as active against cephaloridine as against benzylpenicillin or ampicillin. 3. The enzyme has a net positive charge at pH8.0 and a molecular weight of about 14000. 4. An approximate amino acid composition of the enzyme is reported.
D000596:Amino Acids / Q000032:analysis; D000667:Ampicillin; D002498:Centrifugation; D002509:Cephaloridine; D002850:Chromatography, Gel; D004755:Enterobacteriaceae / Q000201:enzymology*; D008970:Molecular Weight; D010400:Penicillin G; D010405:Penicillinase / Q000032:analysis / Q000302:isolation & purification*
D000596:Amino Acids; D000667:Ampicillin; D010405:Penicillinase; D002509:Cephaloridine; D010400:Penicillin G