Degradation of phenylalanine and tyrosine by Sporobolomyces roseus.
Ammonia-lyase activity for l-phenylalanine, m-hydroxyphenylalanine and l-tyrosine was demonstrated in cell-free extracts of Sporobolomyces roseus. Cultures of this organism converted dl-[ring-(14)C]phenylalanine and l-[U-(14)C]tyrosine into the corresponding cinnamic acid. Tracer studies showed that these compounds were further metabolized to [(14)C]protocatechuic acid. Benzoic acid and p-hydroxybenzoic acid were intermediates in this pathway. Washed cells of the organism readily utilized cinnamic acid, p-coumaric acid, caffeic acid, benzoic acid and p-hydroxybenzoic acid. Protocatechuic acid was the terminal aromatic compound formed during the metabolism of these compounds. The cells of S. roseus were able to convert m-coumaric acid into m-hydroxybenzoic acid, but the latter compound, which accumulated in the medium, was not further metabolized. 4-Hydroxycoumarin was identified as the product of o-coumaric acid metabolism by this organism.
D001565:Benzoates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D002396:Catechols / Q000096:biosynthesis; D002474:Cell-Free System; D002854:Chromatography, Paper; D002934:Cinnamates / Q000378:metabolism; D003374:Coumarins / Q000096:biosynthesis; D007259:Infrared Rays; D007783:Lactones / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D013053:Spectrophotometry; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism*; D014466:Ultraviolet Rays
D001565:Benzoates; D002247:Carbon Isotopes; D002396:Catechols; D002934:Cinnamates; D003374:Coumarins; D007783:Lactones; D002245:Carbon Dioxide; D014443:Tyrosine; D010649:Phenylalanine