Mitotic apparatus: the selective extraction of protein with mild acid.
The treatment of isolated mitotic apparatus with mild (pH 3) hydrochloric acid results in the extraction of less than 10 percent of its protein, accompanied by the selective morphological disappearance of the microtubules. The same extraction can be shown to dissolve outer doublet microtubules from sperm flagella. A protein with points of similarity to the flagellar microtubule protein is the major component of the extract from mitotic apparatus.
D000818:Animals; D004447:Echinodermata; D005260:Female; D006018:Glycols; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D008938:Mitosis; D009940:Organoids / Q000032:analysis*; D009941:Organomercury Compounds; D010063:Ovum / Q000166:cytology*; D011506:Proteins / Q000032:analysis*; D014020:Tissue Extracts; D014461:Ultracentrifugation
D006018:Glycols; D007202:Indicators and Reagents; D009941:Organomercury Compounds; D011506:Proteins; D014020:Tissue Extracts; D006851:Hydrochloric Acid