Cellular differentiation and the aging process in cartilaginous tissues. Mucopolysaccharide synthesis in cell cultures of chondrocytes.
Primary cell cultures of differentiated chondrocytes were shown to produce chondroitin-4-sulfate as the predominant mucopolysaccharide, with suggestive evidence for the synthesis of keratan sulfate and possibly chondroitin-6-sulfate. Chicken embryonic cartilage was shown to be composed mainly of chondroitin-4-sulfate, with a small amount of chondroitin-6-sulfate, but essentially no keratan sulfate. These findings were compared to the data of others, and a hypothesis explaining the aging process in cartilage in terms of cellular differentiation was presented.
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D002642:Chick Embryo; D002807:Chondroitin / Q000032:analysis / Q000096:biosynthesis*; D046508:Culture Techniques; D004586:Electrophoresis; D007259:Infrared Rays; D013057:Spectrum Analysis; D013431:Sulfates / Q000032:analysis
D013431:Sulfates; D002807:Chondroitin