Production of highly labeled adenoviruses.
A method is described for increasing the incorporation of radioactive thymidine into adenovirus deoxyribonucleic acid by the use of amethopterin. In addition, a modified procedure is presented for the preparation of highly purified adenoviruses. This procedure, which employs enzymatic digestion of cellular debris, obviates the necessity for fluorocarbon treatment of crude virus suspensions, and routinely provides excellent recovery of virus.
D000256:Adenoviridae / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004279:DNA, Viral / Q000378:metabolism; D006867:Hydrolases / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology; D008854:Microscopy, Electron; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014776:Virus Cultivation
D004279:DNA, Viral; D014316:Tritium; D006867:Hydrolases; D013936:Thymidine; D008727:Methotrexate