The effects of ovariectomy and injected oestradiol monobenzoate on polypeptide metabolism in the hypothalamus.
1. The activities of two groups of peptidases separated from a homogenate of rabbit hypothalamus were determined (a) in adult female animals, (b) in ovariectomized animals and (c) in intact female animals after injection of oestradiol monobenzoate (15-120mug.). 2. Ovariectomy decreased the enzyme activity initially; the activity in the particulate group of enzymes subsequently returned to normal whereas the activity of the supernatant fraction was less than normal 8 months after operation. 3. Injection of oestradiol monobenzoate increased the enzyme activity in the supernatant fraction to that observed in pregnant animals and in suckled lactating animals. 4. There is a correlation between changes in enzyme activity of the supernatant fraction and conditions that are known to influence gonadotrophin secretion.
D000818:Animals; D002369:Castration; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010053:Ovary / Q000502:physiology; D010121:Oxytocin / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits
D010455:Peptides; D004958:Estradiol; D010121:Oxytocin; D010447:Peptide Hydrolases