Viral events necessary for the induction of interferon in chick embryo cells.
Temperature-sensitive mutants of Sindbis virus were employed to investigate the nature of the viral event(s) which induces chick-embryo cells to produce interferon. Chick embryo cells induced by the parental heat-resistant strain of Sindbis virus produced essentially equal amounts of interferon at 29 and 42 C. An RNA(-) and three RNA(+) strains [temperature-sensitive mutants unable (RNA(-)) and able (RNA(+)) to make ribonucleic acid] produced interferon at 29 C but not at 42 C. It is concluded that viral RNA per se and the replication of viral RNA do not induce interferon production by chick embryo cells.
D000818:Animals; D001103:Arboviruses / Q000502:physiology; D002642:Chick Embryo; D046508:Culture Techniques; D007372:Interferons / Q000096:biosynthesis*; D009154:Mutation; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis* / Q000494:pharmacology; D013696:Temperature; D014779:Virus Replication
D012367:RNA, Viral; D007372:Interferons