Exchangeability of radioactive acetylcholine with the bound acetylcholine of synaptosomes and synaptic vesicles.
1. The exchangeability with added radioactive acetylcholine of the acetylcholine in isolated presynaptic nerve terminals (synaptosomes) and isolated synaptic vesicles was studied by a Sephadex-column method. 2. A substantial proportion of the synaptosomal acetylcholine is exchangeable with added radioactive acetylcholine. It is liberated by hypo-osmotic shock and ultrasonic treatment, and behaves as though it occupies the cytoplasmic compartment of synaptosomes. 3. Methods of isolating vesicles from hypo-osmotically ruptured synaptosomes in optimum yield are discussed. 4. The acetylcholine of synaptic vesicles isolated on a sucrose density gradient is released by hypo-osmotic conditions, suggesting that it is enclosed by a semi-permeable membrane; however, it is not easily released by ultrasonic treatment. 5. Added radioactive acetylcholine does not exchange with vesicular acetylcholine under a variety of different conditions. These include addition of ATP and Mg(2+), and pre-loading of the synaptosome with radioactive acetylcholine before hypo-osmotic rupture. This failure to exchange is discussed in terms of the possible storage mechanism of vesicular acetylcholine.
D000109:Acetylcholine / Q000378:metabolism*; D000255:Adenosine Triphosphate; D000818:Animals; D001692:Biological Transport; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000378:metabolism*; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000378:metabolism; D006168:Guinea Pigs; D008274:Magnesium; D008566:Membranes; D008722:Methods; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism*; D009995:Osmosis; D010539:Permeability; D011188:Potassium / Q000378:metabolism; D013569:Synapses / Q000166:cytology / Q000378:metabolism*; D014465:Ultrasonics
D002247:Carbon Isotopes; D000255:Adenosine Triphosphate; D008274:Magnesium; D000109:Acetylcholine; D011188:Potassium