Cyclic changes in enzyme activity in synchronized mammalian cell cultures.
Activity of the enzymes glucose-6-phosphate dehydrogenase and lactate dehydrogenase increases intermittently in synchronized cultures of Chinese hamster cells. During G(1) phase (3 hours after mitosis), midway through S phase (7 hours after mitosis) and again in late S phase (10 hours after mitosis), rapid increases in activity were observed and correlated with a net increase in cell size and total protein. The evidence suggests that this is a general phenomenon which affects a whole population of proteins and may be the expression of an endogenous cellular rhythm.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D046508:Culture Techniques; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008938:Mitosis; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism
D011506:Proteins; D012313:RNA; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D003078:Colchicine