The glycerol teichoic acid of walls of Staphylococcus lactis I3.
1. The teichoic acid from walls of Staphylococcus lactis I3 was isolated by extraction with trichloroacetic acid and shown to contain glycerol, N-acetylglucosamine, phosphate and d-alanine in the molecular proportions 1:1:2:1. The alanine is attached to the polymer through ester linkages. 2. Hydrolysis with acid gave alanine, glucosamine and glycerol diphosphates. Under mild acid conditions a repeating unit was produced; this consists of glycerol diphosphate joined through a phosphodiester group to N-acetylglucosamine. 3. Hydrolysis with alkali gave glycerol diphosphates, saccharinic acid and two phosphodiesters containing glucosamine whose structures were elucidated; these both contain glucosamine 1-phosphate, and N-acetylglucosamine 1-phosphate was isolated by a degradative procedure. 4. The unusual properties of the teichoic acid are explained by a polymeric structure in which N-acetylglucosamine 1-phosphate is attached through its phosphate to glycerol phosphate. 5. The biosynthetic implications of this structure are discussed.
D000143:Acids; D000409:Alanine / Q000032:analysis; D000468:Alkalies; D002473:Cell Wall / Q000032:analysis*; D004952:Esters; D005944:Glucosamine / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D010428:Pentosephosphates / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D013210:Staphylococcus / Q000032:analysis*; D014238:Trichloroacetic Acid
D000143:Acids; D000468:Alkalies; D004952:Esters; D010428:Pentosephosphates; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D014238:Trichloroacetic Acid; D005944:Glucosamine; D000409:Alanine; D005990:Glycerol