Production of streptococcal M-typing antisera. I. Antigenic response in different breeds of rabbits.
M-antigens of seven M-types of group A streptococci were used to compare antigenic response in six breeds of rabbits: New Zealand White, New Zealand Red, Dutch, Googy, California, and Palomino. No appreciable differences among breeds were demonstrated. M-types used for immunization varied considerably in their ability to evoke a response of an antibody which met rigid criteria for streptococcal M-typing antisera. The importance of the injection and bleeding schedule and absorption conditions for preparing type-specific absorbed antisera were demonstrated. Evaluating and processing sera from individual animals at an optimal time rather than using pooled sera enhanced the possibilities for preparing satisfactory type-specific antisera.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D007106:Immune Sera; D007114:Immunization; D008722:Methods; D011817:Rabbits; D012703:Serotyping; D013291:Streptococcus / Q000145:classification*
D000941:Antigens; D007106:Immune Sera