Analysis of the visible spectra of some sperm-whale ferrimyoglobin derivatives.
The visible spectra of sperm-whale metmyoglobin and its fluoride, hydroxide, cyanide, azide, cyanate, formate, acetate, thiocyanate and nitrite derivatives were measured and resolved into Gaussian components. A linear correlation between the intensity of the band at 18500cm.(-1) and the magnetic susceptibility is found, suggesting that the band is the beta-band of the low-spin form. The analysed intensity of this band can be used to determine the relative proportions of the two spin states present. The spectra of the pure high-spin and low-spin components of each derivative can be reconstructed. Correlations between the energy of the near-infrared band and the visible bands of the high-spin form, and between the position and intensity of the 16000cm.(-1) band, are demonstrated. The relationship between the position of the Soret band and the magnetic susceptibility can be explained in terms of the equilibrium between spin states.
D000085:Acetates; D000818:Animals; D001386:Azides; D002591:Cetacea; D003485:Cyanates; D003486:Cyanides; D005459:Fluorides; D005561:Formates; D006878:Hydroxides; D008280:Magnetics; D009211:Myoglobin / Q000032:analysis*; D009573:Nitrites; D013053:Spectrophotometry; D013861:Thiocyanates
D000085:Acetates; D001386:Azides; D003485:Cyanates; D003486:Cyanides; D005561:Formates; D006878:Hydroxides; D009211:Myoglobin; D009573:Nitrites; D013861:Thiocyanates; D005459:Fluorides