Detection and incidence of specific species of spoilage bacteria on fish. I. Methodology.
The ability of Pseudomonas putrefaciens to form H(2)S was found to serve as a singularly useful criterion of identity for this species and was used to directly enumerate the organism from haddock fillets by the use of pour plates of Peptone-Iron Agar. Subsurface colonies appear intensely black, whereas surface colonies are black or gray. A highly sensitive soft-agar-gelatin overlay technique has been found useful for directly determining the numbers of weakly and strongly proteolytic organisms from fish tissue.
D003470:Culture Media; D005396:Fish Products / Q000032:analysis*; D005516:Food Microbiology; D007501:Iron; D008722:Methods; D010447:Peptide Hydrolases / Q000302:isolation & purification; D010461:Peptones; D011549:Pseudomonas / Q000302:isolation & purification*; D013440:Sulfides / Q000302:isolation & purification
D003470:Culture Media; D010461:Peptones; D013440:Sulfides; D007501:Iron; D010447:Peptide Hydrolases