The glycerol teichoic acid from walls of Staphylococcus epidermidis I2.
1. Walls of Staphylococcus epidermidis I2 contain 30% (w/w) of a glycerol teichoic acid containing phosphate, d-alanine and d-glucose in the molecular proportions 1:0.25:0.50. 2. The teichoic acid was isolated by extraction with trichloroacetic acid and with dilute aqueous NN-dimethylhydrazine at pH7, and was shown to be a (1-->3)-linked poly(glycerol phosphate) containing beta-d-glucopyranosyl and d-alanyl ester substituents. 3. 2-O-beta-d-Glucopyranosylglycerol was isolated and characterized as its crystalline hexa-O-acetate. 4. Unlike that of certain other bacteria, the peptidoglycan component of the wall is not solubilized by NN-dimethylhydrazine. 5. The membrane teichoic acid is also a (1-->3)-linked poly(glycerol phosphate) but contains a smaller proportion of glucosyl substituents.
D000409:Alanine / Q000032:analysis; D002473:Cell Wall / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D005994:Glycerophosphates / Q000032:analysis; D008566:Membranes / Q000032:analysis; D010428:Pentosephosphates / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012995:Solubility; D013210:Staphylococcus / Q000032:analysis*; D014238:Trichloroacetic Acid
D004187:Disaccharides; D005994:Glycerophosphates; D010428:Pentosephosphates; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D014238:Trichloroacetic Acid; D005947:Glucose; D000409:Alanine; D005990:Glycerol