The metabolism of [Me-14C]choline in the brain of the rat in vivo.
[Me-(14)C]Choline was injected intracerebrally into the adult rat, and its uptake into the lipids and their water-soluble precursors in brain was studied. The radioactivity could be detected only in the choline-containing lipids and was confined to the base choline. The results indicated that initial phosphorylation of the free choline followed by the formation of CDP-choline and the subsequent transfer of the phosphorylcholine to a diglyceride is one of the principal routes by which choline lipids in brain are formed. Further evidence for this was obtained in experiments in which either phosphoryl[Me-(14)C]choline or [(32)P]orthophosphate was injected and the radioactivity in the choline-containing water-soluble and lipidbound components studied.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D002794:Choline / Q000378:metabolism*; D007267:Injections; D010710:Phosphates / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D010759:Phosphorus Isotopes; D051381:Rats
D002247:Carbon Isotopes; D010710:Phosphates; D010713:Phosphatidylcholines; D010743:Phospholipids; D010759:Phosphorus Isotopes; D002794:Choline