Reovirus-induced ribonucleic acid polymerase.
A virus-induced ribonucleic acid (RNA) polymerase activity was found in L cells infected with type 3 reovirus. Most of the enzyme is associated with the "large particle" fraction of the infected cells. The enzyme first appeared at 3 to 5 hr after infection and increased in amount until 7 to 9 hr. All four ribonucleoside triphosphates are incorporated in vitro into an acid-insoluble form by the enzyme. The major part of the product formed in vitro is a double-stranded RNA indistinguishable from viral RNA by electrophoresis on polyacrylamide gel. Approximately 40% of the product is a single-stranded RNA of relatively small molecular weight. More than 95% of the nucleotides incorporated into double-stranded RNA by the enzyme are bound in internal 3'-5'-phosphodiester linkages extending back from both 3'- and 5'-termini of the RNA strands.
D000179:Acrylates; D002247:Carbon Isotopes; D004586:Electrophoresis; D004790:Enzyme Induction; D005782:Gels; D007739:L Cells; D008970:Molecular Weight; D009711:Nucleotides / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000302:isolation & purification; D012367:RNA, Viral / Q000096:biosynthesis; D012087:Reoviridae; D014316:Tritium
D000179:Acrylates; D002247:Carbon Isotopes; D005782:Gels; D009711:Nucleotides; D012367:RNA, Viral; D014316:Tritium; D012316:RNA Nucleotidyltransferases