Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.
Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 x 10(6) daltons, respectively, or a total of 15 x 10(6) daltons.
D000179:Acrylates; D004586:Electrophoresis; D005782:Gels; D005827:Genetics, Microbial; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000032:analysis*
D000179:Acrylates; D005782:Gels; D012367:RNA, Viral