The effect of ribonuclease on rat-liver ribosomes.
1. Rat-liver ribosomes lose about 50% of their amino acid-incorporating activity when preincubated with ribonuclease. 2. This preincubation results also in loss of about 50% of the original protein content and 75% of the RNA. 3. Ribosomes sedimented by ultracentrifugation, after preincubation with ribonuclease, show negligible contamination by crystalline enzyme. 4. Washing of ribosomes treated with ribonuclease releases further protein, restoring the original RNA/protein ratio. 5. The washed particle is again capable of promoting amino acid incorporation. 6. Examination of ribosomes treated with ribonuclease in the analytical ultracentrifuge reveals destruction of ribosomes, disappearance of dimers and a decrease in the sedimentation coefficient of monomers. 7. Washed ribosomes consist of even smaller particles with a sedimentation coefficient 60s.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D003460:Crystallization; D003470:Culture Media; D003864:Depression, Chemical; D005260:Female; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014461:Ultracentrifugation
D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D011506:Proteins; D012313:RNA; D012260:Ribonucleases; D007930:Leucine