The effect of adenosine triphosphate on porphyrin excretion and on glycine metabolism in Rhodopseudomonas spheroides.
1. ATP, GTP, CTP and UTP at concentrations of 1mm markedly decrease the amount of coproporphyrin excreted by Rhodopseudomonas spheroides illuminated in a medium containing glycine, succinate and fumarate. 2. The effect of ATP is decreased if ethionine is also added to the medium. 3. Evidence is presented showing that ATP is taken up by the organisms from the medium. 4. ATP is shown to have a marked effect on the utilization of glycine. In the presence of ATP the incorporation of the methylene carbon atom of glycine into the fatty acid moieties of the phospholipids is greatly increased, and more of the carboxyl carbon atom is lost, probably as carbon dioxide. 5. ATP has little effect on the utilization of succinate or fumarate. 6. The possible significance of these results with regard to the control by ATP of porphyrin synthesis and excretion and glycine metabolism is discussed.
D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D000605:Amino Alcohols / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D003470:Culture Media; D003597:Cytosine Nucleotides / Q000494:pharmacology; D003864:Depression, Chemical; D005001:Ethionine / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis; D005650:Fumarates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000494:pharmacology; D008027:Light; D008055:Lipids / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D011166:Porphyrins / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000378:metabolism*; D013268:Stimulation, Chemical; D013386:Succinates / Q000378:metabolism; D013997:Time Factors; D014500:Uracil Nucleotides
D000605:Amino Alcohols; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D003597:Cytosine Nucleotides; D005227:Fatty Acids; D005650:Fumarates; D006150:Guanine Nucleotides; D008055:Lipids; D010743:Phospholipids; D011166:Porphyrins; D013386:Succinates; D014500:Uracil Nucleotides; D000255:Adenosine Triphosphate; D005998:Glycine; D005001:Ethionine