Improved method for determination of the position of double bonds in polyenoic fatty acid esters.
An improved method is described for determination of the position of double bonds in polyenoic fatty acid methyl esters. Partial reduction with hydrazine is employed without prior hydrolysis to the free fatty acid, and the resulting monoenes are identified by reductive ozonolysis.
D002849:Chromatography, Gas; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D006834:Hydrazines; D008722:Methods; D010084:Oxidation-Reduction; D010126:Ozone
D004952:Esters; D005227:Fatty Acids; D006834:Hydrazines; D010126:Ozone