Cell growth and division. IV. Determination of volume growth rate and division probability.
Volume growth rate and division probability functions for mammalian cells have been determined as functions of cell volume with good reproducibility and statistical precision using Coulter volume spectrometry and the equations of the Bell model. Results are compared with independent measurements on synchronous cultures. The slow rate of volume dispersion requires that the growth rate F(tau, V) be closely proportional to volume for cells of a given age. However, when F(tau, V) is averaged over the age distribution of a population in balanced exponential growth to give the growth rate function f(V), the latter may rise more steeply than V.
D000818:Animals; D002455:Cell Division; D006224:Cricetinae; D046508:Culture Techniques; D008962:Models, Theoretical