Inhibitory effect of 2-deoxy-d-glucose on the formation of the cell wall in yeast protoplasts.
2-Deoxy-d-glucose (2DG) acted as a competitive inhibitor of the synthesis of cell wall components in Saccharomyces cerevisiae protoplasts. The synthesis of fibrillar glucan cell wall component was inhibited at a glucose to 2DG ratio of 4:1 in the cultivating medium. The completion of the formation of cell wall by the synthesis of the amorphous mannan-protein cell wall component was inhibited at a glucose to 2DG ratio of about 20:1. The inhibition could be reversed by increasing the glucose to 2DG ratio in the nutrient medium. No incorporation of 2DG into fibrillar glucan cell wall component was observed.
D002473:Cell Wall / Q000254:growth & development; D005947:Glucose / Q000494:pharmacology; D006601:Hexoses / Q000494:pharmacology*; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000096:biosynthesis; D011523:Protoplasts / Q000187:drug effects*; D012440:Saccharomyces / Q000187:drug effects*
D006601:Hexoses; D011134:Polysaccharides; D005947:Glucose