Relationship between acetylcholine synthesis and its concentration in rat cerebral cortex.
1. The synthesis of carbon-14 labelled acetylcholine ((14)C-ACh) from carbon-14 uniformly labelled glucose (U.L. (14)C-glucose) under different conditions has been studied.2. Increasing KCl concentration from 4 mM to 31 mM was associated with a large increase in the amount of (14)C-ACh in the medium and a significant decrease in the amount of (14)C-ACh in the tissue. This effect was observed in slices and minces but not in homogenate.3. Increasing KCl concentration from 4 mM to 31 mM resulted in the release of pre-formed (14)C-ACh.4. Elevated pre-incubation levels of ACh in slices incubated in 4 mM KCl medium was associated with a decrease in the formation of (14)C-ACh.5. These findings are consistent with the hypothesis that the concentration of ACh in the vicinity of the site of synthesis regulates the rate of ACh formation.
D000109:Acetylcholine / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D002247:Carbon Isotopes; D002540:Cerebral Cortex / Q000032:analysis / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009020:Morphine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats
D002247:Carbon Isotopes; D009020:Morphine; D005947:Glucose; D000109:Acetylcholine; D011188:Potassium