Deoxyribonucleic acid methylase activity in pea seedlings.
Deoxyribonucleic acid methylase activity has been detected in a preparation of disrupted nuclei prepared from pea seedlings. S-Adenosyl-L-methionine acted as a donor of methyl groups, and the product of the reaction was identified as 5-methylcytosine. The reaction had a sharp temperature optimum at about 30 degrees C and was unusual in that the DNA methylase was able to methylate DNA in the crude extract.
D002854:Chromatography, Paper; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases; D008715:Methionine; D008745:Methylation; D010945:Plants, Edible / Q000201:enzymology* / Q000378:metabolism; D012260:Ribonucleases; D012639:Seeds; D014166:Transferases / Q000378:metabolism*
D004247:DNA; D008715:Methionine; D014166:Transferases; D003851:Deoxyribonucleases; D012260:Ribonucleases