Phospholipids from Bacillus stearothermophilus.
The lipids of Bacillus stearothermophilus strain 2184 were extracted with chloroform-methanol and separated into neutral lipid and three phospholipid fractions by chromatography on silicic acid columns. The phospholipids were identified by specific staining reactions on silicic acid-impregnated paper, by chromatography of alkaline and acid hydrolysis products, and by determination of acyl ester:glycerol:nitrogen:phosphorus molar ratios. The total extractable lipid was 8% of the dry weight of whole cells and consisted of 30 to 40% neutral lipid and 60 to 70% phospholipid. The phospholipid consisted of diphosphatidyl glycerol (23 to 42%), phosphatidyl glycerol (22 to 39%), and phosphatidyl ethanolamine (21 to 32%). The concentrations of diphosphatidyl glycerol and phosphatidyl glycerol were lower in 2-hr cells than in 4- and 8-hr cells. Whole cells were fractionated by sonic treatment and differential centrifugation. The total lipid content, expressed in per cent of dry weight of each fraction was: whole protoplasts, 10%; membrane fraction, 18%; 30,000 x g particulate fraction, 22%; and 105,000 x g particulate fraction, 26%. The relative phospholipid concentrations in each fraction were about the same. As had been previously reported, none of the phospholipid was stable to alkaline hydrolysis.
D000468:Alkalies; D001407:Bacillus / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D002498:Centrifugation; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004952:Esters / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D008055:Lipids / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis; D011523:Protoplasts / Q000032:analysis; D014465:Ultrasonics
D000468:Alkalies; D004952:Esters; D008055:Lipids; D010743:Phospholipids; D010758:Phosphorus; D009584:Nitrogen; D005990:Glycerol