Purification and concentration of influenza types A and B by chromatography on calcium phosphate.
A rapid chromatographic method for the isolation of types A and B influenza virus from allantoic fluid was described. The adsorbent was prepared from calcium dihydrogen orthophosphate monohydrate [Ca(H(2)PO(4))(2).H(2)O] by alkali treatment. The addition of sodium trimetaphosphate to the influenza-infected allantoic fluid afforded a 67 to 100% viral recovery and a 26 to 43-fold increase in purity.
D000818:Animals; D002130:Calcium Phosphates; D002642:Chick Embryo; D002845:Chromatography; D008722:Methods; D009975:Orthomyxoviridae / Q000302:isolation & purification*
D002130:Calcium Phosphates