Inducible degradation of hydroxyproline in Pseudomonas putida: pathway regulation and hydroxyproline uptake.
Studies in Pseudomonas putida of the inducible degradation of hydroxyproline to alpha-ketoglutarate have indicated that either of the two epimers, hydroxy-l-proline or allohydroxy-d-proline, acts as an inducer of all the pathway enzymes. In a mutant lacking the first enzyme of the sequence, hydroxyproline-2-epimerase, which interconverts these two hydroxyproline epimers, either epimer is still equally active as an inducer of the remaining three enzymes, suggesting that each epimer has intrinsic inducer activity. The second and third enzymes of the sequence were induced coordinately. The induction process appeared to be insensitive to catabolite repression under a number of experimental conditions. The induced enzymes were stable even under conditions of nitrogen starvation and other conditions designed to increase protein turnover. In addition to inducing the degradative enzymes, the two hydroxyproline epimers were also found to induce an uptake system that concentrates hydroxyproline intracellularly. Either amino acid induced the uptake system for its epimer as well as for itself.
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D000447:Aldehydes; D000619:Aminohydrolases / Q000096:biosynthesis*; D004586:Electrophoresis; D004790:Enzyme Induction; D005947:Glucose / Q000378:metabolism; D005977:Glutarates; D006909:Hydroxyproline / Q000378:metabolism*; D007535:Isomerases / Q000096:biosynthesis*; D009154:Mutation; D009584:Nitrogen / Q000494:pharmacology; D010088:Oxidoreductases / Q000096:biosynthesis*; D011549:Pseudomonas / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D000447:Aldehydes; D005977:Glutarates; D014316:Tritium; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D000619:Aminohydrolases; D007535:Isomerases; D005947:Glucose; D009584:Nitrogen; D006909:Hydroxyproline