Effects of cycloheximide and 5-fluorouracil on formation of low-molecular-weight ribonucleic acid in yeast.
The effects of cycloheximide and 5-fluorouracil on the formation of low-molecular-weight ribonucleic acid (RNA) in yeast were investigated. Both compounds were found to affect the synthesis of low-molecular-weight RNA of ribosomal origin more than transfer RNA but less than the high-molecular-weight ribosomal RNA. 5-Fluorouracil-containing transfer RNA was separated from normal transfer RNA by chromatography on diethylaminoethyl cellulose at 80 C in the presence of 7 m urea.
D000225:Adenine / Q000378:metabolism; D002247:Carbon Isotopes; D002845:Chromatography; D002850:Chromatography, Gel; D003513:Cycloheximide / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000032:analysis; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D012313:RNA / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000032:analysis / Q000096:biosynthesis; D012270:Ribosomes; D012440:Saccharomyces / Q000187:drug effects* / Q000378:metabolism; D014508:Urea
D002247:Carbon Isotopes; D009711:Nucleotides; D010710:Phosphates; D010759:Phosphorus Isotopes; D012313:RNA; D014508:Urea; D012343:RNA, Transfer; D003513:Cycloheximide; D008715:Methionine; D007930:Leucine; D000225:Adenine; D005472:Fluorouracil