Hormonal steroids in the tissue of induced rat mammary tumours.
The possible presence of steroids in the tissue of induced hormone-dependent rat mammary tumours was investigated. The method used involves a preliminary extraction of tumours followed by chemical separation and thin-layer chromatography. The identified compounds were cholesterol, androst-4-ene-3,17-dione, 5beta-androst-1-ene-3,17-dione, androsta-1,4-diene-3,17-dione and oestrone. This is the first report of the presence of these steroids in the tissue of an experimental tumour of a non-endocrine organ. In particular 5beta-androst-1-ene-3,17-dione has not previously been identified from natural sources.
D015068:17-Ketosteroids / Q000032:analysis*; D000731:Androstanes / Q000032:analysis*; D000818:Animals; D001551:Benz(a)Anthracenes; D002784:Cholesterol / Q000032:analysis*; D004970:Estrone / Q000032:analysis* / Q000096:biosynthesis; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats
D015068:17-Ketosteroids; D000731:Androstanes; D001551:Benz(a)Anthracenes; D004970:Estrone; D002784:Cholesterol