Chloride fluxes in crab muscle fibres.
1. Isotopic techniques were used to study the efflux of Cl(-) from single cannulated muscle fibres of the crab Maia squinado.2. The efflux of (36)Cl(-) was found to be exponential with steady rate constants ranging from 0.01 to 0.07 min(-1). The steady efflux was ca. 1000-2000 pM cm(-2) sec(-1). The efflux was slightly depressed by K(+)-free saline and was unaffected by 40 mM-K(+) saline and NO(3) (-) saline. The influx and efflux of Cl(-) were presumed independent.3. The membrane conductance calculated from (36)Cl(-) flux data was consistent with that determined by the method of electrotonic spread for other marine crustacean muscle fibres. Cl(-) accounted for most of the membrane conductance.
D000818:Animals; D002463:Cell Membrane Permeability; D002712:Chlorides / Q000378:metabolism*; D003445:Crustacea; D004058:Diffusion; D004553:Electric Conductivity; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011868:Radioisotopes; D013696:Temperature; D013997:Time Factors
D002712:Chlorides; D009566:Nitrates; D011868:Radioisotopes; D011188:Potassium