Total synthesis of acetate from CO2. II. Purification and properties of formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. K(m) values were: adenosine triphosphate (ATP), 6.25 x 10(-4)m; formate, 1.67 x 10(-3)m; and tetrahydrofolate, 1.00 x 10(-3)m; at pH 8.0. There was a requirement for divalent metals and specifically for ATP.
D000085:Acetates / Q000096:biosynthesis*; D000255:Adenosine Triphosphate / Q000378:metabolism; D003013:Clostridium / Q000201:enzymology*; D005492:Folic Acid / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D007700:Kinetics; D008025:Ligases / Q000302:isolation & purification; D013696:Temperature
D000085:Acetates; D005561:Formates; D000255:Adenosine Triphosphate; D005492:Folic Acid; D008025:Ligases