Histone synthesis in vitro by cytoplasmic microsomes from HeLa cells.
HeLa cell microsomes incorporate labeled amino acids in vitro into acid-soluble proteins which have the same electrophoretic mobility as histones isolated from tile purified HeLa cell nuclei. The capacity to Svnthesize histones in vitro is dependent on deoxyribonucleic acid synthesis in the cells from which the microsonal fraction is prepared.
D001120:Arginine / Q000378:metabolism; D002247:Carbon Isotopes; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004586:Electrophoresis; D006367:HeLa Cells / Q000378:metabolism*; D006657:Histones / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D014316:Tritium
D002247:Carbon Isotopes; D006657:Histones; D014316:Tritium; D004247:DNA; D001120:Arginine; D007930:Leucine; D008239:Lysine