Ultramicrofluorimetric determination of calcium in plasma.
A precise and reproducible method is described for the ultramicro fluorimetric determination of calcium in plasma, based upon the formation of a fluorescent complex between calcium and calcein at a strongly alkaline pH. In a group of normal subjects, the mean concentration of plasma calcium was 9.66 mg/100 ml with a normal range of 8.9 to 10.4 mg/100 ml.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002118:Calcium / Q000097:blood* / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D005260:Female; D005470:Fluorometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D008832:Microchemistry; D008875:Middle Aged
D002364:Caseins; D002118:Calcium