Estimating the size and concentration of unicellular microorganisms by light scattering.
The optical density of suspensions of microorganisms was measured at two different positions in a spectrophotometer cuvette compartment. From these two measurements, the particle size and the particle concentration could be estimated. Estimates of particle numbers were not significantly different from those obtained by direct microscopic counting.
D001419:Bacteria; D001699:Biometry; D056890:Eukaryota; D005658:Fungi; D008829:Microbiology; D055095:Optics and Photonics; D013053:Spectrophotometry