Exopenicillinase synthesis in Staphylococcus aureus.
In Staphylococcus aureus, penicillinase remaining cell-bound (60 to 75% of original total) after treatment with citrate does not become exopenicillinase. Exopenicillinase in these cells appears only under conditions permitting de novo penicillinase synthesis. By use of (14)C-labeled cells, it was shown that exopenicillinase consists of newly synthesized molecules which have not equilibrated with preformed membrane-bound enzyme.
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002701:Chloramphenicol / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D010405:Penicillinase / Q000096:biosynthesis* / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D013210:Staphylococcus / Q000201:enzymology* / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D002951:Citrates; D011691:Puromycin; D002701:Chloramphenicol; D010405:Penicillinase