Vaccinia neutralization test using a radioisotope assay for virus-induced enzyme activity.
Vaccinia virus induction of a metabolic activity in host cell cultures forms the basis of a new assay for neutralizing antibodies. A direct relationship between the amount of vaccinia virus infecting cell cultures and the induced incorporation of tritium-labeled thymidine into the acid-insoluble fraction of the cells provided an indicator system. A liquid scintillation spectrometer was used to determine radioactivity associated with cell materials, and it provided a method for partial automation of an immunological procedure. Reproducibility of the method was satisfactory, and agreement with conventional vaccinia serum neutralization tests was demonstrated.
D000596:Amino Acids / Q000378:metabolism; D001331:Automation; D002247:Carbon Isotopes; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009500:Neutralization Tests; D011874:Radiometry; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014616:Vaccinia virus / Q000276:immunology*
D000596:Amino Acids; D002247:Carbon Isotopes; D014316:Tritium; D013936:Thymidine