The interaction between alpha-2-macroglobulin and cationic aspartate aminotransferase.
On starch-gel or polyacrylamide-gel electrophoresis of human serum, a supernumerary zone of aspartate aminotransferase activity may be demonstrated, migrating with the slow alpha(2) protein zone. This appearance is due only to cationic aspartate aminotransferase, bound by alpha(2)-macroglobulin. The binding is strongly potentiated by dilute borate buffers.
D000179:Acrylates; D001219:Aspartate Aminotransferases / Q000097:blood*; D001797:Blood Protein Electrophoresis; D001881:Borates; D002021:Buffers; D005782:Gels; D008259:Macroglobulins; D011108:Polymers; D011485:Protein Binding; D013213:Starch
D000179:Acrylates; D001881:Borates; D002021:Buffers; D005782:Gels; D008259:Macroglobulins; D011108:Polymers; D013213:Starch; D001219:Aspartate Aminotransferases