Cytotoxic effects of leukocyres triggered by complement bound to target cells.
Chromium-51-labeled chicken erythrocytes (E), treated with rabbit anti-Forssman antibody (A) and the first four (C1-4) or the first seven (C1-7) components of human complement (C), released isotope upon exposure to human leukocytes. Isotope release from EACJ-7 cells proceeded more rapidly and was more extensive than that from EACI-3 cells. Lysis of these cells was suppressed by pretreatment of leukocytes with antimycinA. Monocyte-enriched leukocyte preparations affected both types of target cell-complement intermediates, whereas purified lymphocytes lysed EACI-7 cells but not EACI-3 cells.
D000818:Animals; D000906:Antibodies; D000968:Antimycin A / Q000494:pharmacology; D002645:Chickens; D002859:Chromium Isotopes; D003165:Complement System Proteins / Q000494:pharmacology*; D004912:Erythrocytes; D006801:Humans; D007962:Leukocytes; D008214:Lymphocytes; D009000:Monocytes; D011817:Rabbits
D000906:Antibodies; D002859:Chromium Isotopes; D000968:Antimycin A; D003165:Complement System Proteins