The synthesis and turnover of rat liver peroxisomes. II. Turnover of peroxisome proteins.
After preliminary experiments had established that the injection of Triton WR-1339 necessary for the separation of lysosomes and peroxisomes did not affect the turnover rate of catalase, the decay of (3)H-leucine incorporated into peroxisomes was studied in whole particles and in protein subfractions. It was shown that peroxisomes are destroyed in a completely random way, probably as wholes since the apparent half-life was the same for all subfractions, about 3(1/2) days. In agreement with the results of Price et al. (11), the half-life of catalase derived from the rate of recovery from aminotriazole inhibition was about 11(1/2) days, as was the apparent half-life of the heme prosthetic groups measured with (14)C-alpha-aminolevulinic acid. Guanidino-labeled arginine gave an apparent half-life of 2(1/2) days with large statistical uncertainty. Either the leucine label was reutilized very extensively in our animals and the true half-life of peroxisomes is 1(1/2) days, or the prosthetic groups of catalase turn over more rapidly than the protein part of the molecule.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005260:Female; D007930:Leucine / Q000378:metabolism; D007982:Levulinic Acids / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D009940:Organoids / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D014230:Triazoles / Q000494:pharmacology; D014316:Tritium
D007982:Levulinic Acids; D011506:Proteins; D014230:Triazoles; D014316:Tritium; D001120:Arginine; D002374:Catalase; D007930:Leucine