Arsenic-lipid complex formatinon during the active transport of arsenate in yeast.
In studying formation of an arsenic-lipid complex during the active transport of (74)As-arsenate in yeast, it was found that adaptation of yeast to arsenate resulted in cell populations which showed a deficient inflow of arsenate as compared to the nonadapted yeast. Experiments with both types of cells showed a direct correlation between the arsenate taken up and the amount of As-lipid complex formed. (74)As-arsenate was bound exclusively to the phosphoinositide fraction of the cellular lipids. When arsenate transport was inhibited by dinitrophenol and sodium azide, the formation of the As-lipid complex was also inhibited. Phosphate did not interfere with the arsenate transport at a non-inhibitory concentration of external arsenate (10(-9)m). The As-adapted cells but not the unadapted cells were able to take up phosphate when growing in the presence of 10(-2)m arsenate.
D000220:Adaptation, Biological; D001151:Arsenic / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D010710:Phosphates / Q000378:metabolism; D011868:Radioisotopes; D012440:Saccharomyces / Q000187:drug effects / Q000378:metabolism*
D001386:Azides; D003486:Cyanides; D004140:Dinitrophenols; D008055:Lipids; D010710:Phosphates; D011868:Radioisotopes; D001151:Arsenic