The effect of tropolone on the formation of 3,4-dihydroxyphenylacetic acid and 4-hydroxy-3-methoxyphenylacetic acid in the brain of the mouse.
1. The development of a very sensitive and specific fluorimetric assay for 3,4-dihydroxyphenylacetic acid has made it possible to measure how inhibitors of the enzyme catechol-O-methyl transferase affect the relative concentrations of this acid and its O-methylated derivative 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid) in the brains of mice treated with L-3,4-dihydroxyphenylalanine or probenecid.2. It was found that tropolone and tropolone-4-acetamide reduce the concentration of homovanillic acid in the brains of the treated mice to an extent dependent on the dose.3. The concentration of 3,4-dihydroxyphenylacetic acid in the brain was increased by the administration of tropolone or tropolone-4-acetamide but the dose and response were not simply related to one another.4. The results suggest that, in vivo, the formation of 3,4-dihydroxyphenylacetic acid is not always a simple alternative to the formation of homovanillic acid when the enzyme catechol-O-methyl transferase is inhibited.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003516:Cycloparaffins / Q000494:pharmacology*; D004295:Dihydroxyphenylalanine / Q000494:pharmacology; D005260:Female; D005470:Fluorometry; D007659:Ketones / Q000494:pharmacology*; D051379:Mice; D010648:Phenylacetates / Q000032:analysis / Q000096:biosynthesis*; D011339:Probenecid / Q000494:pharmacology
D003516:Cycloparaffins; D007659:Ketones; D010648:Phenylacetates; D004295:Dihydroxyphenylalanine; D011339:Probenecid