Response and specificity of antibodies for Shigella flexneri: demonstration of type-specific factors in immunoglobulin G fraction of antiserum.
Considerable amounts of immunoglobulin G (IgG) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of Shigella flexneri O antigens. High yields of IgG antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution on a diethylaminoethyl-Sephadex column with 0.02 m phosphate buffer (pH 6.6) containing 0.1 m NaCl. Specificities of IgG antibodies for six serotypes of S. flexneri were demonstrated to be high as compared with those of whole sera and their IgM antibodies. Type-specific factors for antigens I to VI were shown in each IgG fraction according to serotype employed. Further, in most sera, subtype-specific factors could be detected in the IgG fraction. These results suggest that it would be desirable to use IgG antibodies for the typing of S. flexneri.
D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D007106:Immune Sera / Q000032:analysis; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D011817:Rabbits; D012703:Serotyping; D012760:Shigella / Q000276:immunology*; D013045:Species Specificity
D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M