Preparation and some properties of L-3-glycerophosphate dehydrogenase from pig brain mitochondria.
1. A method is described for extraction and partial purification of mitochondrial l-3-glycerophosphate dehydrogenase from pig brain. 2. By the criteria that have so far been applied, the extraction and purification procedures do not modify the activity of the enzyme towards artificial electron acceptors. 3. The amounts of acid-liberatable flavine and iron in the preparation were measured. 4. A study was made of the effects of various analogues of l-3-glycerophosphate on the activity of the enzyme.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004579:Electron Transport; D005415:Flavins / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis / Q000302:isolation & purification*; D005994:Glycerophosphates / Q000378:metabolism; D066298:In Vitro Techniques; D007501:Iron / Q000032:analysis; D008928:Mitochondria / Q000201:enzymology*; D013552:Swine
D005415:Flavins; D005994:Glycerophosphates; D007501:Iron; D005993:Glycerolphosphate Dehydrogenase