Some effects of chlorambucil on enzymes of glutathione metabolism in drug-sensitive and -resistant strains of the Yoshida ascites sarcoma.
1. Yoshida ascites cells from a strain sensitive to chemotherapy with alkylating agents contained elevated activities of the two enzymes directly responsible for glutathione synthesis, in comparison with a resistant cell strain. The activities of the glutathione-degrading enzyme gamma-glutamyltranspeptidase were comparable in both cell strains. 2. After parenteral administration of chlorambucil to rats carrying either strain of tumour, the activities of the glutathione-synthetic enzymes increased in the sensitive cells, but remained unchanged in the resistant cells. Drug treatment was without effect on the gamma-glutamyltranspeptidase activity of either cell strain. 3. The activities of a number of enzymes concerned in the oxidoreduction of glutathione remained unchanged after the administration of chlorambucil to rats carrying either strain of cells.
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002699:Chlorambucil / Q000627:therapeutic use*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D051381:Rats; D012517:Sarcoma, Yoshida / Q000188:drug therapy / Q000201:enzymology*
D002699:Chlorambucil; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D010544:Peroxidases; D005980:Glutathione Reductase; D000217:Acyltransferases; D008025:Ligases; D005978:Glutathione