Density-gradient and chromatographic fraction of leptospiral lipase.
Fractionation of leptospiral lipase by CsCl density gradients and G-200 Sephadex chromatography yielded five active protein peaks. Two were obtained from the density gradients and three from G-200 Sephadex columns. Esterase activity of these fractions was demonstrated by electrophoretic examination. Several protein bands were visible when disc electrophoresis was performed on the respective fractions. Lipolytic and esterolytic activities were both present, and the overlapping of these activities was discussed.
D001426:Bacterial Proteins / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004589:Electrophoresis, Disc; D007919:Leptospira / Q000201:enzymology*; D008049:Lipase / Q000032:analysis* / Q000378:metabolism
D001426:Bacterial Proteins; D008049:Lipase