Determination of linkages in beta-D-Glucans from Phaseolus aureus by exo-beta-(1 to 3)-D-glucanase.
An alkali-insoluble glucan synthesized from UDP-d-glucose by the particulate enzyme system from Phaseolus aureus is hydrolyzed by a highly purified exo-beta-(1 --> 3)-d-glucanase to d-glucose, to the extent of 91% in 24 hr. The alkali-insoluble glucan from GDP-d-glucose formed by the particulate enzyme system from the same plant which is known to be (from chemical data) a beta-(1 --> 4)-d-glucan (cellulose) is not acted upon by this glucanase.
D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006026:Glycoside Hydrolases; D010944:Plants; D011134:Polysaccharides; D014500:Uracil Nucleotides / Q000378:metabolism
D002247:Carbon Isotopes; D011134:Polysaccharides; D014500:Uracil Nucleotides; D005954:Glucosephosphate Dehydrogenase; D006026:Glycoside Hydrolases; D005947:Glucose