Gas chromatographic analysis of acidic indole auxins in Nicotiana.
Acidic indole auxins have been extracted from N. glauca, N. langsdorffii and their 2 tumor-prone 4n- and 2n-hybrids. After purification of the extracts and thin-layer chromatography, acidic indoles were subjected to esterification and gas chromatography. The esters of 4 indole acids were detected and determined: indole-3-acetic acid, indole-3-carboxylic acid, indole-3-propionic acid and indole-3-butyric acid. The indolic nature of fractionated samples was confirmed by spectrophotofluorometry and the physiological significance of the indole esters proven in a biotest. A substantial increase in extractable indole-3-butyric acid in the tumor-prone hybrids suggests an additional pathway of auxin synthesis in these tissues.
D001681:Biological Assay; D002087:Butyrates / Q000032:analysis; D002849:Chromatography, Gas; D005470:Fluorometry; D006824:Hybridization, Genetic; D007210:Indoleacetic Acids / Q000032:analysis; D007211:Indoles / Q000032:analysis*; D008722:Methods; D010937:Plant Growth Regulators / Q000032:analysis* / Q000302:isolation & purification; D010941:Plant Tumors; D010947:Plants, Toxic; D013053:Spectrophotometry; D014026:Nicotiana / Q000032:analysis*
D002087:Butyrates; D007210:Indoleacetic Acids; D007211:Indoles; D010937:Plant Growth Regulators