Studies on S-adenosylmethionine-magnesium protoporphyrin methyltransferase in Euglena gracilis strain Z.
1. An enzyme that methylates magnesium protoporphyrin was detected in extracts of light-grown and dark-grown cells of Euglena gracilis. The activity in light-grown cells is two to three times that in cells grown in the dark. 2. The activity is mainly located in the chloroplast fraction from light-grown cells and in proplastids in dark-grown cells. However, in cells grown either in the light or dark, about 15-20% is found in particle-free supernatant. 3. The chloroplast methylating enzyme was solubilized by the action of Tween 80 and partially purified. The properties were investigated. 4. From experiments in which etiolated cells were illuminated in the presence of inhibitors of chloroplast or cytoplasmic protein synthesis, it appears that the methylating enzyme is made on cytoplasmic ribosomes.
D002736:Chloroplasts / Q000201:enzymology; D003593:Cytoplasm; D003624:Darkness; D005055:Euglena / Q000201:enzymology*; D007700:Kinetics; D008027:Light; D008274:Magnesium / Q000378:metabolism; D008745:Methylation; D011166:Porphyrins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013501:Surface-Active Agents; D014166:Transferases / Q000096:biosynthesis / Q000378:metabolism*
D011166:Porphyrins; D013501:Surface-Active Agents; D014166:Transferases; D008274:Magnesium