Cationic protein-bearing granules of polymorphonuclear leukocytes: separation from enzyme-rich granules.
The cationic, antibacterial proteins of polymorphonuclear leukocytes are associated with a unique subcellular particle that is separable through zonal density gradient centrifugation from acid phosphatase-containing particles as well as from particles that contain alkaline phosphatase and lysozyme. Normal macrophages, macrophages stimulated by bacillus Calmette-Guérin, and liver cells lack this particle and the associated group of cationic proteins. Particles physically and biochemically similar to slower sedimenting enzyme-rich particles of polymorphonuclear leukocytes are shared by all the tlhree cell types.
D000135:Acid Phosphatase; D000469:Alkaline Phosphatase; D000818:Animals; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003594:Cytoplasmic Granules; D004586:Electrophoresis; D007962:Leukocytes / Q000032:analysis* / Q000201:enzymology; D008099:Liver / Q000166:cytology; D008264:Macrophages; D009113:Muramidase; D011506:Proteins; D011817:Rabbits
D011506:Proteins; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D009113:Muramidase