Radiation-induced breaks of DNA in cultured mammalian cells.
Mouse leukemic cells (L5178Y) in suspension culture were irradiated and the extent of single-strand breaks and double-strand cuts of DNA was estimated by sucrose gradient centrifugation. The radiation produced 3.0 single-strand breaks per cell (G(1) stage) per rad and approximately 0.3 double-strand breaks per cell (G(1) stage) per rad.
D000818:Animals; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004273:DNA, Neoplasm / Q000528:radiation effects*; D006863:Hydrogen-Ion Concentration; D007942:Leukemia, Experimental; D051379:Mice; D011830:Radiation Effects; D013936:Thymidine / Q000032:analysis; D014316:Tritium; D014529:Uridine
D002247:Carbon Isotopes; D004273:DNA, Neoplasm; D014316:Tritium; D013936:Thymidine; D014529:Uridine