Changes in nuclear basic proteins during pseudorabies virus infection.
As a preliminary study to investigation of the possible role played by basic proteins in the genetic regulation of virus-infected cells, acid-extractable proteins synthesized during pseudorabies virus infection were investigated. The synthesis of histones was found to decrease in a gradual manner, and arrest was complete by 6 hr after infection. Five virus-induced acid-extractable proteins appeared in nuclei of infected cells after 4 hr of infection. Four of these proteins were virus structural proteins; one was not. All these proteins contained tryptophan and, therefore, were not "classic" histones.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D002247:Carbon Isotopes; D002460:Cell Line / Q000378:metabolism; D046508:Culture Techniques; D004589:Electrophoresis, Disc; D006564:Herpesviridae / Q000378:metabolism*; D006657:Histones / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D007668:Kidney; D008239:Lysine / Q000378:metabolism; D011492:Protein Hydrolysates / Q000378:metabolism; D011557:Pseudorabies; D011817:Rabbits; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
D002247:Carbon Isotopes; D006657:Histones; D011492:Protein Hydrolysates; D014764:Viral Proteins; D014316:Tritium; D014364:Tryptophan; D001120:Arginine; D008239:Lysine