Biosynthesis of puromycin in Streptomyces alboniger. Enzymatic methylation of O-demethylpuromycin.
D002247:Carbon Isotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D008715:Methionine / Q000378:metabolism*; D008745:Methylation; D008781:Methyltyrosines / Q000096:biosynthesis; D011691:Puromycin / Q000096:biosynthesis* / Q000138:chemical synthesis / Q000302:isolation & purification; D013053:Spectrophotometry; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism*
D002247:Carbon Isotopes; D008781:Methyltyrosines; D011691:Puromycin; D008715:Methionine; D014166:Transferases