Studies of Raman spectra of water solutions of adenosine tri-, di-, and monophosphate and some related compounds.
Using a He-Ne CW laser source together with a digital photon counting system, we have obtained well resolved Raman spectra for adenosine mono-, di-, and triphosphate (AMP, ADP, ATP) in aqueous solution. Spectra of these compounds were studied as a function of pH from pH = 0.5 to 13.5 and between 550 and 1700 cm(-1). It was found possible to distinguish spectroscopically between the three phosphates over the pH range studied. A qualitative analysis of vibrational modes responsible for various spectral lines is given. Lines at about 960 and 1100 cm(-1) were found to be good indications of the degree of ionization of the terminal phosphate group.
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D010710:Phosphates; D013057:Spectrum Analysis / Q000295:instrumentation
D000227:Adenine Nucleotides; D010710:Phosphates; D000255:Adenosine Triphosphate