Hormone storage granules in the beef anterior pituitary. I. Isolation, ultrastructure, and some biochemical properties.
A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mmicro and of small granules is 220 mmicro. The large granules contain growth hormone and prolactin; the small granules contain high concentrations of follicle-stimulating, luteinizing, and thyroid-stimulating hormones. An alkaline protease with a pH optimum of 8.3 is associated with the small granule fraction.
D000818:Animals; D002417:Cattle; D003594:Cytoplasmic Granules / Q000032:analysis* / Q000201:enzymology; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification; D013006:Growth Hormone / Q000302:isolation & purification; D007986:Luteinizing Hormone / Q000302:isolation & purification; D008854:Microscopy, Electron; D010447:Peptide Hydrolases; D010902:Pituitary Gland / Q000166:cytology* / Q000201:enzymology; D010908:Pituitary Hormones, Anterior / Q000302:isolation & purification*; D011388:Prolactin / Q000302:isolation & purification; D013972:Thyrotropin / Q000302:isolation & purification
D010908:Pituitary Hormones, Anterior; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone; D010447:Peptide Hydrolases