A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenase.
1. Inhibition of ox liver glutamate dehydrogenase with N-(N'-acetyl-4[(35)S]-sulphamoylphenyl)maleimide (ASPM) is more specific at pH7.3 than at pH6.9. At pH7.3 inhibition accompanies the incorporation at 1 mole of ASPM residues into about 53000g. of protein. 2. Digestion of the modified protein with chymotrypsin and trypsin yields a unique radioactive peptide. 3. Acid hydrolysis of 1 mole of this peptide yields 1 mole of N(in)-succin-2-yl-lysine. The in-amino group of a lysyl residue is thus the site of modification of the protein. 4. The sequence containing the modified lysyl residue is: [Formula: see text] where Asx respresents either aspartic acid or asparagine.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin; D005969:Glutamate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008239:Lysine / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011758:Pyrroles; D013460:Sulfur Isotopes; D014357:Trypsin
D010455:Peptides; D011758:Pyrroles; D013460:Sulfur Isotopes; D005969:Glutamate Dehydrogenase; D002918:Chymotrypsin; D014357:Trypsin; D008239:Lysine