Role of erythrocytes and serum in the nutrition of Rickettsia quintana.
Rickettsia quintana grew readily on blood-agar base when the following conditions and supplements were supplied: (i) aerobic conditions; (ii) increased CO(2) tension; (iii) crystalline hemoglobin or hemin, but not protoporphyrin; and (iv) a colloidal "detoxifying agent," such as starch or charcoal. Serum was not required nor did it enhance growth when all of the aforementioned components were supplied.
D001769:Blood; D002245:Carbon Dioxide; D002850:Chromatography, Gel; D003470:Culture Media; D004912:Erythrocytes / Q000032:analysis; D006418:Heme; D006454:Hemoglobins; D008970:Molecular Weight; D010313:Partial Pressure; D011166:Porphyrins; D012281:Rickettsia / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine
D003470:Culture Media; D006454:Hemoglobins; D011166:Porphyrins; D002245:Carbon Dioxide; D012710:Serum Albumin, Bovine; D006418:Heme