Evidence for excision of ultraviolet-induced pyrimidine dimers from the DNA of human cells in vitro.
Within 12-24 hr after human cells were irradiated with ultraviolet light, approximately 50% of the ultraviolet-induced pyrimidine dimers were lost from the DNA. Pyrimidine dimers were found in the TCA-soluble fraction of ultraviolet-irradiated cells at 24 hr. Excess thymidine, caffeine, or hydroxyurea had no effect on the loss of pyrimidine dimers from the DNA of ultraviolet-irradiated cells.
D000650:Amnion; D002110:Caffeine / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008168:Lung; D011108:Polymers; D011743:Pyrimidines; D011830:Radiation Effects; D013936:Thymidine / Q000494:pharmacology; D013997:Time Factors; D014316:Tritium; D014466:Ultraviolet Rays
D011108:Polymers; D011743:Pyrimidines; D014316:Tritium; D002110:Caffeine; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea