Arbovirus plaquing in a clonal line (PS Y-15) of porcine kidney.
Plaque development of 42 arbovirus serotypes, representing 17 serogroups, was studied in a clonal line (PS Y-15) of porcine kidney. The arboviruses plaqued within 3 to 7 days, with plaque diameters ranging from 1 to 5 mm. Mouse mortality and cell infectivity end points were compared with 24 arboviruses. Arbovirus LD(50) end points in mice exceeded the porcine kidney monolayer plaque-forming unitage end point with all 24 viruses compared.
D000818:Animals; D001103:Arboviruses / Q000302:isolation & purification*; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004668:Encephalitis Viruses / Q000302:isolation & purification; D007668:Kidney; D012672:Semliki forest virus / Q000302:isolation & purification; D013552:Swine; D014776:Virus Cultivation; D015005:Yellow fever virus / Q000302:isolation & purification