Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens.
Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens V-1 is temperature-sensitive. At 25 C and 30 C, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 C, although bacterial growth was not inhibited, phage release was suppressed. Phage synthesis was induced by heat shock, 42 C for 30 min, ultraviolet irradiation, and mitomycin C. Induction by ultraviolet light was unusual-an immediate rise in phage titer followed irradiation. A large increase occurred after a 90-min latent period. The lysogenic strain was cured of the phage by incubation at 37 C, and the cured strain produced plant tumors.
D001433:Bacteriolysis; D001435:Bacteriophages; D005827:Genetics, Microbial; D008242:Lysogeny; D008937:Mitomycins / Q000494:pharmacology; D010941:Plant Tumors; D012231:Rhizobium / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays
D008937:Mitomycins