Direct measurement of potential difference across the human red blood cell membrane.
The electrical potential difference across the human red cell membrane has been measured directly. A biological amplifier with neutralized input capacity was used. Human red cells in modified Ringer solution were impaled individually with 3 M KCl-filled glass microelectrodes. Movements of the microelectrodes were effected by Leitz micromanipulators. Results showed a potential difference of -8.0 +/- 0.21 (SEM) mv, the inside being negative with respect to the outside. This value is approximately that calculated by using the Nernst equation considering the intracellular and extracellular chloride concentrations.As a control, similar measurements were made on nylon microcapsules containing hemoglobin. The measured potential of -0.52 +/- 0.02 (SEM) mv, which agreed very well with the value calculated on the basis of Donnan equilibrium, was much smaller in magnitude as compared to the results for the red cell, and there was evidence of fixed charges on the microcapsule membrane. There was no evidence of this in the case of the red cell.
D004566:Electrodes; D004594:Electrophysiology; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D008433:Mathematics; D008564:Membrane Potentials; D008846:Micromanipulation