Bioassay for hamycin and amphotericin B in serum and other biological fluids.
A bioassay suitable for measuring concentrations of the polyene antifungal agents hamycin and amphotericin B in biological fluids is described. By using Paecilomyces varioti as the indicator organism, sensitivity of the bioassay was found to be in the range of 0.01 to 0.02 mug/ml. A linear dose-response curve was obtained with amphotericin B; the curve for hamycin was curvilinear. In a series of assays, hamycin serum levels in the range of 0.01 to 3.5 mug/ml were measured; with amphotericin B, serum levels in the range of 0.015 to 0.175 mug/ml were measured in patients receiving orthodox intravenous medication and as high as 9.0 mug/ml in one patient treated with extraordinarily high doses of the drug.
D000666:Amphotericin B / Q000097:blood* / Q000134:cerebrospinal fluid / Q000652:urine; D000818:Animals; D000935:Antifungal Agents / Q000032:analysis / Q000097:blood* / Q000134:cerebrospinal fluid / Q000652:urine; D001681:Biological Assay; D006801:Humans; D008722:Methods; D051379:Mice; D003904:Mitosporic Fungi / Q000187:drug effects
D000935:Antifungal Agents; D000666:Amphotericin B