Cellulolysis by Mucor pusillus.
Culture filtrates of Mucor pusillus NRRL 2543 contained hydrolytic enzymes that attacked native cellulose, acid-swollen cellulose, carboxymethylcellulose, and cellobiose. The distribution profiles of cellulolytic and beta-glucosidase activities after gel filtration on Sephadex G-75 showed the presence of several active peaks. Glucose was the only product of hydrolysis when native cellulose was used as the substrate. Acid-swollen cellulose, when treated with cellulase free of beta-glucosidase activity, gave rise to glucose, cellobiose, and at least two higher molecular weight components which were also hydrolyzed in turn to cellobiose and glucose. The presence of a multiple cellulolytic enzyme system was indicated, the components of which may have specific roles in the degradation of cellulose.
D002482:Cellulose / Q000378:metabolism*; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D005959:Glucosidases; D006026:Glycoside Hydrolases; D008747:Methylcellulose / Q000378:metabolism; D009089:Mucor / Q000201:enzymology*
D002482:Cellulose; D008747:Methylcellulose; D005959:Glucosidases; D006026:Glycoside Hydrolases