Uptake of glucose-l-14C by Pullularia pullulans.
Pullularia pullulans cells were grown in a medium containing yeast extract, malt extract, glucose, and nutrient salts, in addition to glucose-1-(14)C. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total radioactive carbon added to the culture medium, the neutral lipid fractions contained 24.8%, whereas the phospholipid portions contained only 2.1%. The largest amount (16.5%) of (14)C among the neutral lipids was found in the fraction containing the free sterols. Among the phospholipids, the largest amount (1.1%) was found in phosphatidylserine and phosphatidylethanolamine. The second largest amount (9.6%) of the total (14)C used was found in trehalose, followed by carbon dioxide (7.3%).
D002247:Carbon Isotopes; D002845:Chromatography; D003470:Culture Media; D004187:Disaccharides / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D003904:Mitosporic Fungi / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D010860:Pigments, Biological / Q000302:isolation & purification; D013261:Sterols / Q000378:metabolism
D002247:Carbon Isotopes; D003470:Culture Media; D004187:Disaccharides; D008055:Lipids; D010743:Phospholipids; D010860:Pigments, Biological; D013261:Sterols; D005947:Glucose