L-cell virion ribonucleic acid.
"L-cell virion," obtained from chronically infected 929 L-cell lines, was purified on 70% sucrose pads and its ribonucleic acid (RNA) was extracted and analyzed on sucrose gradients. Two RNA components were isolated from the virion, a rapidly sedimenting species (82S) and a more slowly sedimenting species (6S). When the fast-moving component was heated to 45 C, it yielded a homogeneous peak which now sedimented at approximately 72S. Upon heating to 70 C, the peak dissociates into a smaller homogeneous RNA species with a sedimentation coefficient of approximately 33S. Ribonuclease treatment and base composition analysis revealed that the fast-moving species is a single-stranded molecule. Base ratio analysis also revealed that the small (6S) RNA component is similar in its base composition to L-cell ribosomal RNA and distinctly different from the base composition of the large (72S) RNA component. Actinomycin D inhibits production of the L-cell virion by the L-cell line.
D002499:Centrifugation, Density Gradient; D003609:Dactinomycin / Q000494:pharmacology; D006358:Hot Temperature; D007739:L Cells; D010759:Phosphorus Isotopes; D012328:RNA Viruses / Q000032:analysis* / Q000187:drug effects; D012343:RNA, Transfer; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012270:Ribosomes; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D012343:RNA, Transfer; D014529:Uridine