Differentiation of phospholipases A in mitochondria and lysosomes of rat liver.
Highly purified mitochondria from rat liver contain a phospholipase A that catalyzes removal of 2-fatty acids, with a pH optimum above pH 8.0. Lysosomal preparations appeared to have two phospholipases A associated with them, one with a pH optimum at about pH 4.0, the second between pH 6.0 and 7.0. Mitochondrial phospholipase A hydrolyzed exogenous phospholipid as fast as or faster than endogenous phospholipid. The difference in specific radioactivity of (14)C-ethanolamine-labeled endogenous mitochondrial phospholipid before and after incubation indicates that a fraction of mitochondrial phosphatidyl ethanolamine is hydrolyzed more rapidly than the mitochondrial phospholipids as a whole. Acyl bond hydrolysis of exogenous and endogenous phospholipid by mitochondria was stimulated by free fatty acid, Ca(++), or in certain cases, monoacyl phospholipids or by treatments that disrupt the mitochondrial membrane. Of various fatty acids tested, lauric, myristic, oleic, and linoleic were most effective. ADP and ATP inhibited mitochondrial phospholipase, probably because they compete for Ca(++). Mg(++) also behaved as a competitive inhibitor; the effect was overcome by relatively little Ca(++).
D000227:Adenine Nucleotides / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002247:Carbon Isotopes; D002794:Choline; D003864:Depression, Chemical; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010740:Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010759:Phosphorus Isotopes; D051381:Rats; D013268:Stimulation, Chemical
D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate; D010740:Phospholipases; D008274:Magnesium; D002794:Choline