Vinblastine-induced precipitation of microtubule protein.
Vinblastine quantitatively precipitates a protein from supernatants obtained from high-speed centrifugation of homogenates of HeLa cells and of pig brain. This protein migrates as a single band on gel electrophoresis, has a mobility identical to that of purified microtubule protein, and-like microtubule protein-binds colchicine. The precipitation is partially inhibited by 0.9 percent NaCl.
D000818:Animals; D001923:Brain Chemistry; D011232:Chemical Precipitation; D003078:Colchicine; D004586:Electrophoresis; D006367:HeLa Cells; D066298:In Vitro Techniques; D011485:Protein Binding; D011506:Proteins; D012996:Solutions; D013552:Swine; D014316:Tritium; D014461:Ultracentrifugation; D014747:Vinblastine / Q000494:pharmacology*
D011506:Proteins; D012996:Solutions; D014316:Tritium; D014747:Vinblastine; D003078:Colchicine