The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis.
The incubation of Delta(7)-cholestenol with a 10000g supernatant or 105000g microsomes in the presence of tritiated water is studied. The reisolated Delta(7)-cholestenol contained up to 0.67g.atom of tritium/mole. This result can best be explained by assuming the reversibility of the reaction Delta(8)-cholestenol right harpoon over left harpoon Delta(7)-cholestenol.
D000818:Animals; D002776:Cholestanes / Q000378:metabolism; D002784:Cholesterol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007535:Isomerases / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D013261:Sterols / Q000378:metabolism; D014316:Tritium; D014461:Ultracentrifugation
D002776:Cholestanes; D013261:Sterols; D014316:Tritium; D002784:Cholesterol; D007535:Isomerases