L-asparaginase production by Erwinia aroideae.
Maximum yields of 1,250 IU (international unit)/g (dry weight of cells) of L-asparaginase were obtained in 8 hr from Erwinia aroideae NRRL B-138. Partial purification and concentration of the extracted L-asparaginase yielded a preparation with an activity of 275 IU/ml. Only one L-asparaginase was present as determined by electrophoresis, and the enzyme exhibited a pH optimum of 7.5 and a K(m) of 3 x 10(-3) M.
D001215:Asparaginase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D004586:Electrophoresis; D004885:Erwinia / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration
D001215:Asparaginase