Human oocytes: maturation in chemically defined media.
Human oocytes from ovarian follicles resume meiosis in F10, a defined medium, in numbers comparable to that obtained in medium containing serum. Several simple Krebs-Ringer media also support maturation, which suggests a similarity of nutritional requirements between human and mouse oocytes. Fewer oocytes reach metaphase II when the investing follicular cells are removed prior to culture.
D003470:Culture Media; D005260:Female; D006801:Humans; D008540:Meiosis; D010063:Ovum / Q000166:cytology*
D003470:Culture Media