The nature of the antigenic determinant in a genetic control of the antibody response.
The response of inbred mouse strains to two polypeptides derived from multichain polyprolines, (T,G)-Pro--L and (Phe,G)-Pro--L, is different from the response of the same mouse strains to a similar series of polymers built on multi-poly-D,L-alanyl--poly-L-lysine, although the same short sequences of amino acids are attached to the side chains of the polypeptides in the two series. These results indicate that a portion of the side chain (e.g. polyalanine or polyproline) participates in the antigenic determinant. This was confirmed by studying the response of different mouse strains to two kinds of polypeptides: (T,G)-Pro-A--L 717 and 718 and (T,G)-A-Pro--L 719 and 721. Antibody assay of antisera to (Phe,G)-Pro--L with the cross-reacting antigens (T,G)-Pro--L and (Phe,G)-A-L indicates that different inbred mouse strains make antibodies specific for different parts of the same polypeptide. Thus, antibody from DBA/1 mice reacts almost exclusively with the (Phe,G) sequence, while SJL antisera bind only (T,G)-Pro--L and fail to bind (Phe,G)-A-L. The immune responses to the same amino acids on two different polypeptides (i.e. A--L and Pro--L) appear to be under separate genetic control.
D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000378:metabolism; D001947:Breeding; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005823:Genetics; D007106:Immune Sera / Q000032:analysis / Q000378:metabolism; D007114:Immunization; D007456:Iodine Isotopes; D008297:Male; D051379:Mice / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D011392:Proline; D014316:Tritium
D000596:Amino Acids; D000941:Antigens; D007106:Immune Sera; D007456:Iodine Isotopes; D010455:Peptides; D014316:Tritium; D011392:Proline