Phosphotransferase system of Staphylococcus aureus: its requirement for the accumulation and metabolism of galactosides.
The phosphotransferase system of Staphylococcus aureus was characterized. Mutants defective in enzyme I and heat-stable (HPr) protein as well as in the two components specific to lactose accumulation, factor III and enzyme II, were isolated. Colorimetric assays for each of the components are presented based on the formation of o-nitrophenyl-beta-d-galactoside-6-phosphate by the system and its hydrolysis by the staphylococcal 6-phospho-beta-galactosidase. The components were partially purified and their molecular weights were estimated: enzyme I, 100,000 +/- 15%; HPr, 10,000 +/- 15%; factor III, 30,000 +/- 15%; 6-phospho-beta-galactosidase, 45,000. Enzyme II is a membrane-bound protein.
D002845:Chromatography; D006027:Glycosides / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D008970:Molecular Weight; D009154:Mutation; D010770:Phosphotransferases; D013210:Staphylococcus / Q000201:enzymology* / Q000378:metabolism
D006027:Glycosides; D010770:Phosphotransferases; D007785:Lactose