Partial purification of the anti-inflammatory factor(s) in inflammatory exudate.
1. The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti-inflammatory activity of inflammatory exudates.2. Incubation alone at a temperature above 70 degrees C or with pronase at 37 degrees C destroyed the anti-inflammatory activity of exudate.3. The anti-inflammatory component of exudate was partially precipitated by 50% ammonium sulphate.4. A partial purification process was devised using Sephadex G-150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification.5. Measurements of 11-hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its anti-inflammatory effects.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D001681:Biological Assay; D002351:Carrageenan; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005122:Exudates and Transudates / Q000032:analysis*; D005938:Glucocorticoids / Q000032:analysis; D008297:Male; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D051381:Rats; D013696:Temperature
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D000644:Quaternary Ammonium Compounds; D002351:Carrageenan