The heparin-protein complex of ox liver capsule. Isolation and chemical characterization.
1. A procedure for the isolation and purification of the heparin-protein complex from ox liver capsule, based on the solubility properties of mucopolysaccharide-cetylpyridinium complexes, is described. 2. The yield of the heparin-protein complex with this method averages 35mg./100g. of dry ox liver capsule. 3. The results of analyses on the polysaccharide show good agreement with values previously published for purified heparin fractions. 4. The amino acid analysis of the protein component shows several similarities to that of chondromucoprotein. 5. The results of beta-carbonyl elimination, either with or without catalytic hydrogenation, and column chromatography after beta-elimination, show that the polysaccharide is covalently bound to the protein.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D001780:Blood Coagulation Tests; D002417:Cattle; D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D003902:Detergents; D006493:Heparin / Q000032:analysis / Q000302:isolation & purification; D006595:Hexosamines / Q000032:analysis; D008099:Liver / Q000032:analysis; D008566:Membranes / Q000032:analysis; D008722:Methods; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D013431:Sulfates / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
D000596:Amino Acids; D003902:Detergents; D006595:Hexosamines; D011506:Proteins; D013431:Sulfates; D014574:Uronic Acids; D002482:Cellulose; D006493:Heparin