Metabolism of D- and L-glyceraldehyde in adipose tissue: a stereochemical probe for glycerokinase activity.
Distributions of (14)C have been determined in free glycerol, in glycerol from triglycerides, in glucose from glycogen, and in lactate after incubation of d-glyceraldehyde-3-(14)C and l-glyceraldehyde-3-(14)C with rat adipose tissue. The distributions are interpreted in terms of presently accepted possible reactions for the initial metabolism of glyceraldehyde. Formation of glycerol-1-(14)C from d-glyceraldehyde-3-(14)C indicates that in adipose tissue glyceraldehyde is reduced to glycerol. Incorporation of (14)C from d-glyceraldehyde-3-(14)C into carbon 3 of the glycerol of triglyceride indicates that d-glyceraldehyde is either phosphorylated or oxidized to d-glyceric acid, or both, in its initial metabolism. Incorporation of (14)C from l-glyceraldehyde-3-(14)C into carbon 3 of glycerol indicates that l-glyceraldehyde is reduced to glycerol, which is phosphorylated and (or) converted to d-glyceric acid via l-glyceric acid. Some (14)C from l-glyceraldehyde-3-(14)C is incorporated into carbon 1 of glycerol of triglycerides and carbon 4 of glycogen; the explanation for this incorporation is uncertain.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002247:Carbon Isotopes; D004822:Epididymis; D005947:Glucose / Q000096:biosynthesis; D005985:Glyceraldehyde / Q000378:metabolism*; D005988:Glyceric Acids / Q000096:biosynthesis; D005990:Glycerol / Q000096:biosynthesis / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis; D008297:Male; D010710:Phosphates / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D013237:Stereoisomerism; D014280:Triglycerides / Q000096:biosynthesis*
D002247:Carbon Isotopes; D005988:Glyceric Acids; D007773:Lactates; D010710:Phosphates; D014280:Triglycerides; D005985:Glyceraldehyde; D006003:Glycogen; D010770:Phosphotransferases; D005947:Glucose; D005990:Glycerol