The distribution of glucose and [14C]glucose between erythrocytes and plasma in the rat.
1. Of the glucose in rat blood 79.8+/-3.3% (s.d.) was in the plasma. The variance was mostly due to differences between rats. 2. The concentration of glucose in erythrocyte water was 51+/-8% (s.d.) of that in plasma water. 3. The ratio (specific radioactivity in plasma)/(specific radioactivity in whole blood), i.e. the P/B ratio, was estimated for glucose at intervals after intravenous injection of [U-(14)C]glucose and [U-(14)C]fructose. The ratio differed from unity by more than the standard error of a single determination of the specific radioactivity of blood or plasma glucose except from 10 to 17min. after injection of [(14)C]glucose and from 22 to 30min. after injection of [(14)C]fructose. At all other times specific radioactivities in blood had to be corrected to give specific radioactivities in plasma. How to do so is described. 4. The P/B ratios were accounted for by a turnover of glucose in erythrocytes of 0.14mumole/min./ml. of erythrocytes. 5. Metabolism of glucose in rat erythrocytes is unlikely to be a major source of lactate.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism*; D006400:Hematocrit; D007700:Kinetics; D007773:Lactates / Q000097:blood; D008297:Male; D008722:Methods; D010949:Plasma; D051381:Rats; D013997:Time Factors
D001786:Blood Glucose; D002247:Carbon Isotopes; D007773:Lactates; D005632:Fructose; D005947:Glucose