Enzyme from calf thymus degrading the RNA moiety of DNA-RNA Hybrids: effect on DNA-dependent RNA polymerase.
An enzyme present in extracts from calf thymus degrades specifically the RNA moiety of DNA-RNA hybrids. Other nucleic acids, such as single- or double-stranded DNA and single- or double-stranded RNA, are not affected to a comparable degree. If prepared free of the hybrid-degrading enzyme, RNA polymerase from calf thymus shows a fivefold increase in activity on denatured DNA as compared to native DNA.
D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004247:DNA; D004279:DNA, Viral; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000037:antagonists & inhibitors*; D012316:RNA Nucleotidyltransferases; D013950:Thymus Gland / Q000201:enzymology*; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D002247:Carbon Isotopes; D004279:DNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D012313:RNA; D004247:DNA; D012316:RNA Nucleotidyltransferases