Studies on the purification of antihemophilic factor (factor 8. I. Precipitation of antihemophilic factor by concanavalin A.
Concanavalin A precipitates antihemophilic factor from normal plasma. Combining this precipitation with other techniques, we were able to separate fractions rich in antihemophilic activity from human plasma with rapidity. The molecular weight of antihemophilic factor was estimated to be greater than 2 million. Presumably, antihemophilic factor is a large glycoprotein.
D000818:Animals; D011232:Chemical Precipitation; D004589:Electrophoresis, Disc; D005169:Factor VIII / Q000302:isolation & purification*; D006023:Glycoproteins; D006801:Humans; D007106:Immune Sera; D066298:In Vitro Techniques; D008722:Methods; D008970:Molecular Weight; D010940:Plant Proteins; D011817:Rabbits; D013053:Spectrophotometry; D014461:Ultracentrifugation
D006023:Glycoproteins; D007106:Immune Sera; D010940:Plant Proteins; D005169:Factor VIII