Mammalian DNA polymerase: separation of binding from incorporation of deoxyribonucleoside triphosphates.
An assay for the binding of deoxyribonucleoside triphosphate with an enzyme-DNA complex has been developed. This binding requires active enzyme and magnesium ion, takes place equally with native or denatured DNA, and may proceed in the absence of demonstrable DNA synthesis. The binding reaction appears to be specific for deoxyribonucleoside triphosphates, and studies on competition indicate that one active site accommodates the four common triphosphates.
D001665:Binding Sites; D002850:Chromatography, Gel; D004247:DNA; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D004273:DNA, Neoplasm; D008274:Magnesium / Q000494:pharmacology; D008722:Methods; D009691:Nucleic Acid Denaturation; D009711:Nucleotides; D014316:Tritium
D004273:DNA, Neoplasm; D009711:Nucleotides; D014316:Tritium; D004247:DNA; D004254:DNA Nucleotidyltransferases; D008274:Magnesium