Crystalline 3-phosphoglycerate kinase from skeletal muscle.
1. A procedure for preparing crystalline 3-phosphoglycerate kinase from rabbit or pig skeletal muscle is presented. 2. The preparation phosphorylates up to 975mumoles of 3-phosphoglycerate/min./mg. at 30 degrees and is not contaminated with myokinase. 3. The enzyme has an estimated molecular weight of 36500+/-1000, and contains three residues each of tyrosine and tryptophan. 4. The preparation is suitable for use in the enzymic procedures for determining ATP, phosphocreatine and 3-phosphoglycerate.
D000818:Animals; D002845:Chromatography; D003460:Crystallization; D008722:Methods; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010735:Phosphoglycerate Kinase / Q000302:isolation & purification*; D011817:Rabbits; D013552:Swine; D014364:Tryptophan / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D014443:Tyrosine; D014364:Tryptophan; D010735:Phosphoglycerate Kinase