RNA synthesis in Chinese hamster cells. II. Increase in rate of RNA synthesis during G1.
Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis.
D000818:Animals; D001345:Autoradiography; D002247:Carbon Isotopes; D003080:Cold Temperature; D006224:Cricetinae; D046508:Culture Techniques; D008715:Methionine / Q000378:metabolism; D008938:Mitosis; D012313:RNA / Q000096:biosynthesis*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D014316:Tritium; D012313:RNA; D008715:Methionine; D014529:Uridine