A rat liver system that catalyses a pyridoxal phosphate-independent alpha beta-eliminto.
1. Attempts were made to demonstrate the presence of pyridoxal phosphate in the rat liver system catalysing the alphabeta-elimination of l-serine O-sulphate. 2. Methods designed to resolve protein-bound cofactor, spectroscopic examination of a purified enzyme system and attempted reactivation of apo-(alanine aminotransferase) failed to demonstrate the presence of pyridoxal phosphate. 3. The activity of the alphabeta-eliminating system remained constant in vitamin B(6)-deficient animals even though the activities of other pyridoxal phosphate-dependent systems fell markedly. 4. The metabolism of l-serine O[(35)S]-sulphate in vivo appears to be normal in vitamin B(6)-deficient animals. 5. No incorporation of tritium into the alphabeta-eliminating system occurred after administration of tritiated pyridoxine to experimental animals.
D000410:Alanine Transaminase; D000818:Animals; D004798:Enzymes; D007538:Isoniazid / Q000494:pharmacology; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D011485:Protein Binding; D011732:Pyridoxal Phosphate / Q000032:analysis; D051381:Rats; D012694:Serine / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013460:Sulfur Isotopes; D014316:Tritium; D026681:Vitamin B 6 Deficiency
D004798:Enzymes; D013431:Sulfates; D013460:Sulfur Isotopes; D014316:Tritium; D012694:Serine; D011732:Pyridoxal Phosphate; D000410:Alanine Transaminase; D007538:Isoniazid