Preparation of crystalline nucleoside diphosphate kinase from baker's yeast and identification of 1-[32P]phosphohistidine as the main phosphorylated product of an alkaline hydrolysate of enzyme incubated with adenosine [32P]triphosphate.
D000096:Acetone; D000255:Adenosine Triphosphate; D000595:Amino Acid Sequence; D001345:Autoradiography; D002482:Cellulose; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D003956:Dialysis; D005374:Filtration; D006639:Histidine / Q000302:isolation & purification; D008239:Lysine / Q000302:isolation & purification; D008722:Methods; D009711:Nucleotides; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000302:isolation & purification; D012440:Saccharomyces / Q000201:enzymology*; D013053:Spectrophotometry
D009711:Nucleotides; D010759:Phosphorus Isotopes; D000096:Acetone; D006639:Histidine; D000255:Adenosine Triphosphate; D002482:Cellulose; D010770:Phosphotransferases; D008239:Lysine