Pancreatic ribonuclease-poly G complexes: complete inhibition of poly U hydrolysis.
Pancreatic ribonuclease forms large complexes with poly G in 0.1 M acetate buffer solutions (pH 5.4). These are largest when the ratio, of ribonuclease to poly G concentration, is slightly less than 2. Under the same conditions lysozyme forms still larger complexes with poly U, and these are largest when the ratio, of lysozyme to poly U concentration, is about 2.5. The ribonuclease in ribonuclease-poly G complexes digests poly U. Free ribonuclease digests the poly U in lysozyme-poly U complexes. However, when the poly G concentration is about an order of magnitude greater than that required to bind all the ribonuclease, lysozyme-bound poly U is not hydrolyzed.
D000085:Acetates; D002021:Buffers; D055598:Chemical Phenomena; D002621:Chemistry; D006150:Guanine Nucleotides; D008027:Light; D009113:Muramidase; D010179:Pancreas / Q000201:enzymology; D011119:Polynucleotides; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D013053:Spectrophotometry; D014500:Uracil Nucleotides
D000085:Acetates; D002021:Buffers; D006150:Guanine Nucleotides; D011119:Polynucleotides; D014500:Uracil Nucleotides; D012260:Ribonucleases; D009113:Muramidase
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5639935
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5696207
J Mol Biol. 1968 Jun 28;34(3):595-608
4938559