Inactivation of bacteriophage lambda containing semiconserved alkylated deoxyribonucleic acid.
Immediate and delayed inactivation of ethylmethane sulfonate (EMS)-treated lambda phage were studied. Phage particles with one alkylated and one intact deoxyribonucleic acid (DNA) strand were obtained by allowing host-modified, EMS-treated phage to undergo one growth cycle in a nonmodifying host and selecting the progeny with semiconserved parental DNA on a restricting host. The results indicate that particles with one alkylated DNA strand are more sensitive to a second treatment with the alkylating agent. When incubated at 37 C, they are subject to inactivation at a rate which is smaller than that of phages containing two alkylated DNA strands. It appears that depurination events in one of the DNA strands of a phage particle are sufficient to cause death.
D000477:Alkylating Agents / Q000494:pharmacology*; D001435:Bacteriophages / Q000187:drug effects*; D003470:Culture Media; D004261:DNA Replication; D004279:DNA, Viral / Q000502:physiology*; D013997:Time Factors; D014774:Virulence / Q000187:drug effects; D014776:Virus Cultivation
D000477:Alkylating Agents; D003470:Culture Media; D004279:DNA, Viral
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