Nucleoside incorporation into strain L cells: inhibition by pleuropneumonia-like organisms.
Contamination of Strain L cell cultures by pleuropneumonia-like organismtis (PPLO) resulted in a complete inhibition of the incorporation of tritiated thymnidine and uridine. Contanminated cultures were characterized by a concentration of PPLO along the margins of the cells and in the intercellular-spaces of the cultures. Autoracliograins of PPLO-contaminated cultures were characterized by exposed silver grains over the margins of the cells. Kanamnycin was efjective in the elimination of PPLO and the restoration of nucleoside incorporation.
D001345:Autoradiography; D046508:Culture Techniques; D007739:L Cells; D009174:Mycoplasma; D013936:Thymidine / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*
D013936:Thymidine; D014529:Uridine