Constitution, viability, and lactate dehydrogenase in stationary-phase L-cell suspension cultures.
Starved suspension cultures of L cells exhibit a plateau phase of short duration followed by loss of key cellular constituents and rapidly decreasing viability. In contrast, regularly fed,. undiluted cultures remain stationary at a high cell density for prolonged periods without structural alterations or loss of viability. The L cells contain a single lactate dehydrogenase isozyme with an electrophoretic mobility similar to that of lactate-dehydrogenase-5. High-density stationary cultures show a tenfold increase of the specific activity of this enzyme and a recurrent biphasic pattern of carbohydrate utilization with, first, production and, later, consumption of lactate.
D003470:Culture Media; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004586:Electrophoresis; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D007739:L Cells; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis
D003470:Culture Media; D007773:Lactates; D012313:RNA; D004247:DNA; D007770:L-Lactate Dehydrogenase; D005947:Glucose