Lability of host-cell DNA in growing cell cultures due to Mycoplasma.
In HeLa-cell cultures chronically infected with Mycoplasma, (PPLO) host-cell DNA is unstable as detected by incorporation of H(3)-or C1 i-thymidine into DNA and subsequent release into the medium as acidsoluble radioactivity. This characteristic can be transmitted to PPLO-free cultures of strain L cells by inoculation with preparations of PPLO from the HeLa cells, although chronically infected cultures of L cells continue to multiply. In addition, virus preparations also may carry PPLO contamination through numerous passages.
D002247:Carbon Isotopes; D002455:Cell Division; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D006367:HeLa Cells; D007739:L Cells; D009174:Mycoplasma; D011874:Radiometry; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D014316:Tritium; D004247:DNA; D013936:Thymidine