Some properties of the lactic dehydrogenase agent of mice.
The lactic dehydrogenase agent was obtained in quantities sufficient for purification studies by growing the virus in Ehrlich ascites tumor-bearing mice. A rapid method of titration of the agent is described. Subsequent to the standard procedure of concentration of virus by treatment with hyaluronidase and centrifugation, lipids were removed by extraction with PE, without major loss of infectivity. Electron microscopic sections of purified preparations contained particles consisting of a dense inner ring of about 25 mmicro and a less dense ring extending to about 50 mmicro. The particles occur frequently in single-membraned vesicles of varying size, and occasionally in large double-membraned bodies. The purified LDH agent did not stimulate the formation of neutralizing antibodies in rabbits and guinea pigs. The crude LDH agent was found to be a low interferon producer. Increased interferon, produced by secondary inoculation with Newcastle disease virus temporarily decreased the titer of the LDH agent. The results of others regarding the nature and the size of the LDH agent are interpreted in regard to the findings presented, and the role of interferon in permanently LDH agent infected mice is discussed.
D000818:Animals; D000941:Antigens; D002286:Carcinoma, Ehrlich Tumor; D006821:Hyaluronoglucosaminidase; D007372:Interferons; D007770:L-Lactate Dehydrogenase; D051379:Mice; D008854:Microscopy, Electron; D009858:Oncogenic Viruses / Q000276:immunology*; D010578:Petroleum; D012260:Ribonucleases; D012997:Solvents; D014461:Ultracentrifugation
D000941:Antigens; D010578:Petroleum; D012997:Solvents; D007372:Interferons; D007770:L-Lactate Dehydrogenase; D012260:Ribonucleases; D006821:Hyaluronoglucosaminidase
Biochim Biophys Acta. 1961 Nov 25;54:210-2
13891872
Virology. 1964 Nov;24:481-3
14227051
Nature. 1964 Apr 25;202:418-9
14152853
Science. 1960 Aug 26;132(3426):545-7
17798830
Proc Soc Exp Biol Med. 1962 Dec;111:749-54
13943920
Cancer Res. 1962 Jun;22:573-80
14008679
Virology. 1964 Nov;24:501-2
14227057
Proc Soc Exp Biol Med. 1964 Nov;117:338-41
14233436
J Natl Cancer Inst. 1964 Oct;33:741-9
14220254
J Cell Biol. 1963 Apr;17:208-12
13986422
Virology. 1963 Oct;21:269-71
14070180
J Exp Med. 1963 May 1;117:735-47
13939042
Virology. 1963 Aug;20:640-2
14059831
Fed Proc. 1962 Jan-Feb;21:19-31
14475273
Biochem J. 1964 Mar;90(3):477-82
5833358
Life Sci. 1963 Jul;(7):479-85
13982837
Virology. 1964 Apr;22:563-7
14166116
Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1015-9
14230330