Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase.
1. Two beta-galactosidases from human small-intestinal mucosa were separated by gel-filtration chromatography and the properties of the two enzymes were studied. Lactose and four hetero beta-galactosides were used as substrates. 2. One of the enzymes was particle-bound and could be partially solubilized with papain. Of the substrates hydrolysed by this enzyme, lactose was hydrolysed most rapidly. This enzyme is thus essentially a disaccharidase and is named lactase. It is presumably identical with the ;lactase 1' described earlier. 3. The other enzyme was mainly soluble and hydrolysed all artificial substrates used, whereas no lactase activity could be detected. This enzyme has therefore been designated hetero beta-galactosidase. 4. p-Chloromercuribenzoate (0.1mm) inhibited the hetero beta-galactosidase completely but did not influence the activity of the lactase. Tris was a competitive inhibitor of both enzymes. 5. The residual lactase activity in the mucosa of lactose-intolerant patients may be exerted by a small amount of remaining lactase as such, or possibly by a third enzyme with a more acid pH optimum.
D002729:Chloromercuribenzoates; D002845:Chromatography; D002850:Chromatography, Gel; D005696:Galactosidases / Q000032:analysis* / Q000037:antagonists & inhibitors; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000037:antagonists & inhibitors; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D007785:Lactose / Q000378:metabolism; D007787:Lactose Intolerance / Q000201:enzymology; D010206:Papain; D014325:Tromethamine
D002729:Chloromercuribenzoates; D014325:Tromethamine; D005696:Galactosidases; D006026:Glycoside Hydrolases; D010206:Papain; D007785:Lactose
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