Properties of 42S and 26S Sindbis viral ribonucleic acid species.
Two species of ribonuclease-sensitive Sindbis viral ribonucleic acids which sedimented at 42S and 26S were studied. 42S RNA, derived either from virions or from viral nucleoids extracted from infected cultures, was converted by heating to an RNA which sedimented at 26S. The sedimentation patterns of 42S RNA and "derived" 26S RNA were similarly affected in low ionic strength buffers. 42S RNA ran as a homogeneous fraction on polyacrylamide gels; the "derived" 26S RNA as well as "natural" 26S RNA from infected cultures showed similar electrophoretic patterns of heterogeneity. A doubling of 3' polynucleotide termini was observed when 42S RNA was heated. Two possibilities concerning the structure of 42S RNA are considered. (i) It may consist of an aggregate of subunits, joined by means of hydrogen bonds to form a complex molecule. (ii) A heat-labile covalent bond of unknown type may link viral RNA subunits. Although 26S RNA from infected cultures and "derived" 26S RNA from 42S RNA behaved in a similar qualitative manner on gels, their sedimentation characteristics were affected differently in low ionic strength buffers. "Natural" and "derived" 26S RNA appear to consist of a population of fragments. and their behavior in gradients and in gels is probably dictated by the experimental conditions of the analytical methods used.
D000818:Animals; D001103:Arboviruses / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D004586:Electrophoresis; D006358:Hot Temperature; D006867:Hydrolases / Q000494:pharmacology; D066298:In Vitro Techniques; D010759:Phosphorus Isotopes; D012313:RNA / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012270:Ribosomes / Q000032:analysis; D012911:Snakes; D013997:Time Factors; D014316:Tritium; D014529:Uridine; D014688:Venoms / Q000494:pharmacology
D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014688:Venoms; D014316:Tritium; D012313:RNA; D006867:Hydrolases; D014529:Uridine
Arch Gesamte Virusforsch. 1967;20(2):225-34
4299727
J Biol Chem. 1961 May;236:1372-9
13767412
Z Naturforsch B. 1959 Jun;14B:370-8
13855323
Virology. 1968 Dec;36(4):696-700
4302009
Biochim Biophys Acta. 1967 Jun 20;142(1):111-27
4292782
J Virol. 1967 Dec;1(6):1168-73
5621488
Virology. 1961 May;14:54-8
13701154
J Virol. 1967 Apr;1(2):374-83
5630381
Proc Natl Acad Sci U S A. 1966 Apr;55(4):974-81
4286764
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511-8
4299948
J Mol Biol. 1967 Jun 28;26(3):373-87
5339793
Biochim Biophys Acta. 1965 Nov 8;108(3):348-54
4286384
Biochem J. 1967 Jan;102(1):251-7
5339944
J Exp Med. 1954 Feb;99(2):183-99
13130793
Nature. 1968 Oct 26;220(5165):396-9
5684887
J Mol Biol. 1968 May 14;33(3):609-23
5700415
Virology. 1969 Jan;37(1):124-31
4302800
Can J Microbiol. 1969 Feb;15(2):215-22
5764278
J Virol. 1968 Oct;2(10):1038-46
5723707
Proc Natl Acad Sci U S A. 1966 Aug;56(2):440-6
5229967
J Gen Virol. 1968 May;2(3):365-75
5675578
Can J Microbiol. 1968 Jan;14(1):45-51
5642006
J Mol Biol. 1966 Jan;15(1):77-91
4287898
Nature. 1967 Jan 28;213(5074):365-7
6067598
Biochem J. 1966 Dec;101(3):721-6
16742451
Virology. 1969 Mar;37(3):367-76
5777557
J Gen Virol. 1968 Sep;3(2):175-84
5698680
J Virol. 1968 Jun;2(6):558-66
5697470