The replacement of the active compounds in a serum-free medium and the serial transfer of L line cells in it.
D000418:Albumins; D003470:Culture Media; D046508:Culture Techniques; D005228:Fatty Acids, Essential; D007739:L Cells; D010461:Peptones
D000418:Albumins; D003470:Culture Media; D005228:Fatty Acids, Essential; D010461:Peptones