The separation and distribution of simple and condensed leucoanthocyanins of the tea plant (Camellia sinensis L.).
1. Leucoanthocyanin monomers of high mobilities in aqueous solvents on thinlayer chromatograms, assumed to be structurally simple, were characteristic of mature bulky tissues, whereas members of lower mobility were confined to young vegetative and floral tissues. 2. Flavylogens were separated by gel filtration on Sephadex columns into monomeric, oligomeric and polymeric fractions. 3. The polymeric fraction from young brown stems was heterogeneous, one-half having a molecular weight of about 3400, one-third a molecular weight between 3600 and 17000, and the remainder a molecular weight of over 17000. 4. Leaves had low flavylogen concentrations; only monomers were present. Stem tissues were rich in polymers, which increased with the age of the young stem and decreased inwards through the wood. The maximal flavylogen concentrations were in the phloem and cambium from mature stems, where all three fractions were richly present. The periderm tissue and, to a lesser extent, the seed coat were characterized by a very high polymer/monomer ratio, exhibiting a much higher degree of polymerization than the wood. Root tissues contained high concentrations of monomers. 5. In general, there was an inverse correlation between the extent of polymerization and the complexity of the monomers present. 6. The results are in favour of the thesis that the function of the flavanols is, after polymerization to condensed tannins, to impregnate dead structural tissues and thereby to protect them from infection and decay.
D000872:Anthocyanins / Q000032:analysis*; D001578:Benzopyrans / Q000032:analysis*; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000032:analysis*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010944:Plants / Q000032:analysis*; D012639:Seeds / Q000032:analysis; D013662:Tea / Q000032:analysis
D000872:Anthocyanins; D001578:Benzopyrans; D005419:Flavonoids; D046911:Macromolecular Substances; D013662:Tea
Biochem J. 1960 Jul;76(1):17-23
16748820
Biochem J. 1962 Feb;82:320-4
14494576
Biochem J. 1958 Oct;70(2):344-9
16748787
Biochem J. 1969 Aug;113(5):741-55
5821006
Biochem J. 1960 Mar;74:607-15
14401811
Biochem J. 1961 Apr;78:834-9
13744043
Nature. 1966 Aug 27;211(5052):944-5
5968301
Nature. 1966 Jan 22;209(5021):368-70
5920237
Nature. 1965 Oct 9;208(5006):151-3
5884249
Biochem J. 1958 Aug;69(4):530-8
13572314
Nature. 1955 Apr 2;175(4457):597
14370179
Biochem J. 1963 Apr;87(1):167-72
16748995