Intramitochondrial localization of delta-aminolaevulate synthetase and ferrochelatase in rat liver.
Intramitochondrial loci for delta-aminolaevulate synthetase and ferrochelatase, the initial and final enzymes in haem synthesis, have been found in rat liver. Two different methods of fractionation were applied to mitochondria: (a) sonication and density-gradient centrifugation; (b) treatment with digitonin and differential centrifugation. Similar results were obtained with each technique. delta-Aminolaevulate synthetase is distributed similarly to two known matrix enzymes, malate dehydrogenase and glutamate dehydrogenase. Ferrochelatase is firmly bound to the the inner mitochondrial membrane. These results are considered in terms of the regulation of haem synthesis and in relation to mitochondrial biogenesis.
D000596:Amino Acids; D000818:Animals; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D004071:Digitalis Glycosides; D005969:Glutamate Dehydrogenase / Q000032:analysis; D006418:Heme / Q000096:biosynthesis; D007982:Levulinic Acids; D008025:Ligases / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008190:Lyases / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D008566:Membranes / Q000032:analysis; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats
D000596:Amino Acids; D004071:Digitalis Glycosides; D007982:Levulinic Acids; D006418:Heme; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D008190:Lyases; D008025:Ligases
J Cell Biol. 1967 Feb;32(2):415-38
10976232
Biochem Biophys Res Commun. 1965 Dec 9;21(5):480-7
5880525
J Biol Chem. 1966 Mar 25;241(6):1359-75
5935350
J Biol Chem. 1966 Jun 25;241(12):2803-9
4957991
Proc Natl Acad Sci U S A. 1966 May;55(5):1217-24
5225520
Ann N Y Acad Sci. 1966 Jul 14;137(2):643-66
4290884
J Cell Biol. 1967 Mar;32(3):719-35
4291912
J Biol Chem. 1967 Oct 25;242(20):4584-6
6061404
J Biol Chem. 1968 Jul 10;243(13):3581-7
5658540
Biochem Biophys Res Commun. 1968 Jun 28;31(6):977-82
5668190
J Cell Biol. 1968 Jul;38(1):158-75
5691970
Proc Natl Acad Sci U S A. 1968 Oct;61(2):598-605
4176482
J Biol Chem. 1969 Mar 25;244(6):1552-62
5773057
J Biol Chem. 1951 Apr;189(2):665-70
14832284
J Biol Chem. 1951 Nov;193(1):265-75
14907713
J Biol Chem. 1954 Jun;208(2):645-61
13174575
Biochim Biophys Acta. 1957 Oct;26(1):22-9
13479456
Ann N Y Acad Sci. 1958 Oct 13;75(1):122-8
13627809
J Histochem Cytochem. 1959 Jul;7(4):257-61
13664976
J Biol Chem. 1961 Apr;236:1173-80
13746277
Biochim Biophys Acta. 1961 Aug 5;51:356-60
13893581
J Biol Chem. 1963 Feb;238:821-7
13949831
Biochem J. 1963 Apr;87:181-5
13972328
Biochim Biophys Acta. 1960 Jul 1;41:185-91
14413186
J Biol Chem. 1949 Feb;177(2):751-66
18110453