Ribosomes: analysis by cesium sulfate gradient centrifugation.
Ribosomes from the cytoplasm of a mammalian tissue, HeLa cells, were partially purified and then concentrated into a zone by prolonged centrifugation in Cs(2)SO(4) solution (density, 1.43 grams per milliliter). Ribosomal preparations treated with bentonite prior to density-gradient centrifugation showed single peaks after centrifugation. Material recovered from the peaks represented aggregates of the original 74S particle.
D000538:Aluminum Silicates; D002498:Centrifugation; D002586:Cesium; D046508:Culture Techniques; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000378:metabolism; D010759:Phosphorus Isotopes; D011874:Radiometry; D012270:Ribosomes; D013057:Spectrum Analysis; D013431:Sulfates; D014316:Tritium; D014633:Valine / Q000378:metabolism
D000538:Aluminum Silicates; D010759:Phosphorus Isotopes; D013431:Sulfates; D014316:Tritium; D002586:Cesium; D014633:Valine; D008239:Lysine