Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants.
1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 10(8) daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3x10(6) and 1.2x10(8) daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.
D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts; D004247:DNA / Q000032:analysis; D004269:DNA, Bacterial; D004279:DNA, Viral; D003720:Densitometry; D007700:Kinetics; D008722:Methods; D008854:Microscopy, Electron; D008928:Mitochondria; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D059828:Plant Cells; D010944:Plants; D011687:Purines / Q000032:analysis; D011744:Pyrimidinones / Q000032:analysis; D013045:Species Specificity; D013997:Time Factors
D004269:DNA, Bacterial; D004279:DNA, Viral; D011687:Purines; D011744:Pyrimidinones; D004247:DNA
Proc Natl Acad Sci U S A. 1963 Dec;50:1164-9
14096192
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1351-8
5237870
Stain Technol. 1964 Mar;39:111-4
14127791
J Mol Biol. 1965 Apr;11:769-74
14338786
Biochim Biophys Acta. 1967 Sep 26;145(2):378-90
6064633
J Mol Biol. 1964 Sep;9:812-24
14216623
Proc Natl Acad Sci U S A. 1968 Sep;61(1):245-52
5246924
J Mol Biol. 1966 Jun;17(2):463-9
5963078
Arch Biochem Biophys. 1957 Oct;71(2):367-76
13471039
Biochim Biophys Acta. 1964 Mar 23;80:391-8
14153841
J Mol Biol. 1965 Feb;11:373-90
14290352
Proc Natl Acad Sci U S A. 1965 Jul;54(1):207-13
5216353
Biochim Biophys Acta. 1968 Apr 22;157(2):333-43
5649909
Plant Physiol. 1959 Jan;34(1):56-61
16655175
Plant Physiol. 1966 Mar;41(3):383-8
16656268
Exp Cell Res. 1966 Jan;41(1):81-98
4952036
Biochim Biophys Acta. 1964 Nov 15;91:477-85
14254019
J Cell Biol. 1965 May;25:327-44
14287184
J Mol Biol. 1968 Feb 14;31(3):627-31
5637203
Exp Cell Res. 1956 Aug;11(2):317-21
13375653
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1214-9
16578578
Biochem J. 1968 Oct;109(4):543-57
4300828
J Mol Biol. 1963 Aug;7:130-41
14062649