Saturated hydrocarbons in bovine liver.
A homologous series of n-alkanes (C(14)-C(33)) and two isoprenoid hydrocarbons, 2,6,10,14-tetramethylhexadecane (phytane) and 2,6,10,14-tetramethylpentadecane (pristane) have been identified in bovine liver. Another branched but non-isoprenoid alkane and three isomers of molecular formula C(20)H(40) were partially identified. Phytane and the C(18)-C(22) and C(29)-C(33)n-alkanes were found to be the major components in liver, suggesting that at least the main hydrocarbon components were derived from various plants in the diet. The hydrocarbons were separated and identified by a series of steps involving solvent extraction, saponification, elution chromatography on alumina and silica gel columns, molecular sieving and by infrared and ultraviolet spectroscopy, followed by combined capillary gas chromatography-mass spectrometry.
D000473:Alkanes / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002845:Chromatography; D002849:Chromatography, Gas; D004032:Diet; D007259:Infrared Rays; D008099:Liver / Q000032:analysis*; D010944:Plants; D013057:Spectrum Analysis; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D000473:Alkanes
Science. 1965 Apr 2;148(3666):77-9
17773778
Science. 1965 Feb 19;147(3660):870-3
17793563
Cancer Res. 1958 Aug;18(7):825-32
13573349
Biochem J. 1952 Oct;52(2):161-8
13018202
Science. 1969 Jan 31;163(3866):467-8
17731762
J Am Oil Chem Soc. 1968 Mar;45(3):144-7
5642087
Arch Biochem Biophys. 1966 Jan;113(1):245-7
5941990
Biochem J. 1967 Aug;104(2):340-52
6048776
Biochem J. 1932;26(2):403-12
16744838
Nature. 1962 Feb 24;193:739-42
13889451
Biochim Biophys Acta. 1965 Oct 4;106(2):386-96
5867698
Acta Chem Scand. 1967;21(10):2887-9
5585687
Biochem J. 1931;25(6):2095-110
16744780
J Bacteriol. 1967 Jun;93(6):1811-8
6025301
Biochem J. 1955 Apr;59(4):684-90
14363164
J Bacteriol. 1967 Aug;94(2):333-43
6039356
Science. 1966 Oct 21;154(3747):399-400
5917089
Biochem J. 1934;28(6):2189-208
16745627
Proc Natl Acad Sci U S A. 1965 Nov;54(5):1406-12
16591314
Science. 1963 May 31;140(3570):974
17836481