Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis.
Seven cistrons in phiX-174 were identified and one in particular was studied intensively: cistron A, which is assigned a protein in the mature phage. Amber mutants in this cistron synthesize a new deoxyribonucleic acid (DNA) form in addition to circular phage DNA upon infection of the restrictive host. This DNA is linear, non-infectious, and single-stranded; it is formed from the phage strand of replicative form phiX-174 DNA. These mutants produce two different defective particles in the restrictive host. One particle contains circular phage DNA but is not infectious; the other contains the new DNA form and is similar to the 70S particles found in wild-type phage lysates. The mutant A gene product acts independently of normal A protein upon mixed infection of the restrictive host with an A mutant and a mutant from any other cistron or wild type.
D001435:Bacteriophages / Q000032:analysis / Q000302:isolation & purification; D003168:Complement Fixation Tests; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D005796:Genes; D006824:Hybridization, Genetic; D009154:Mutation; D011874:Radiometry; D013936:Thymidine; D014316:Tritium; D014764:Viral Proteins / Q000494:pharmacology; D014776:Virus Cultivation
D004279:DNA, Viral; D014764:Viral Proteins; D014316:Tritium; D013936:Thymidine
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