Distribution of granular vesicles in normal and constricted sympathetic neurones.
1. A quantitative analysis has been made of the characteristics of granular vesicles in different parts of normal and ligated post-ganglionic sympathetic neurones supplying the cat spleen. The least diameter of the vesicle membrane and its electron-dense core was measured from electron micrographs with an automated particle size analyser.2. Vesicles of average diameter 443 A, with and without electron-dense cores (192 A), were concentrated in the axon terminals of the spleen, whereas in the cell bodies in the coeliac ganglion and their axons there were only a few large vesicles (787 A) with large granules (562 A).3. Ligation of the splenic nerves for 24 hr resulted in an accumulation proximal to the constriction of granular vesicles significantly larger (688 A) and more completely granulated (478 A) than those in the terminals.4. Reserpine reduced the number of vesicles with granular cores without discrimination between the two populations.5. Since the distribution of noradrenaline in sympathetic neurones can be correlated with the presence of large as well as small granular vesicles, it is suggested that the larger, more granular type found mainly proximally in the neurone is in transit from its perikaryal site of synthesis to the axon terminals, where it is transformed into the smaller synaptic vesicles.
D000818:Animals; D001369:Axons / Q000166:cytology; D002415:Cats; D005725:Ganglia, Autonomic / Q000166:cytology; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000166:cytology; D009474:Neurons / Q000032:analysis / Q000166:cytology*; D009638:Norepinephrine / Q000032:analysis; D012110:Reserpine / Q000494:pharmacology; D013154:Spleen / Q000294:innervation*; D013564:Sympathetic Nervous System / Q000166:cytology*; D013569:Synapses
D012110:Reserpine; D009638:Norepinephrine
J Pharmacol Exp Ther. 1965 Nov;150(2):190-5
5855876
Pharmacol Rev. 1966 Mar;18(1):387-99
5323767
J Anat. 1965 Oct;99(Pt 4):677-89
5867168
Z Zellforsch Mikrosk Anat. 1965 Jul 15;67(2):211-8
5883935
Experientia. 1966 Jan 15;22(1):56
5950315
J Pharmacol Exp Ther. 1966 Nov;154(2):185-99
5950955
Nature. 1966 May 7;210(5036):599-601
6007247
Int J Neuropharmacol. 1966 Jul;5(4):281-5
5965640
Acta Physiol Scand. 1966 Jul-Aug;67(3):278-88
5967594
Proc R Soc Lond B Biol Sci. 1967 Mar 28;167(1008):282-92
4382780
Nervenarzt. 1966 Nov;37(11):493-8
5983176
J R Microsc Soc. 1967;87(2):277-94
6081281
Circ Res. 1967 Dec;21(6):Suppl 3:13-24
4295533
J Pharmacol Exp Ther. 1968 Aug;162(2):203-12
5666978
J Neurochem. 1968 Sep;15(9):925-30
5682510
J Neurochem. 1968 Sep;15(9):931-9
5682511
Z Zellforsch Mikrosk Anat. 1968;87(3):330-44
5707306
J Physiol. 1969 Oct;204(3):593-605
4980932
Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1953;219(1-2):17-22
13099333
J Ultrastruct Res. 1961 Mar;5:51-64
13726332
J Anat. 1962 Oct;96:427-42
13982122
Nature. 1956 Jan 7;177(4497):44-5
13288591
Acta Pharmacol Toxicol (Copenh). 1965;22:277-92
14328200