Adenovirus antibody measured by the passive hemagglutination test.
Lefkowitz, Stanley S. (Variety Children's Research Foundation, Miami, Fla.), Julia A. Williams, Bernard E. Howard, and M. Michael Sigel. Adenovirus antibody measured by the passive hemagglutination test. J. Bacteriol. 91:205-212. 1966.-Rabbits immunized intravenously with adenovirus type 5 antigen were tested for antibody titers by use of the passive hemagglutination test (PHA). Primary and secondary responses were studied, and the class of antibody was determined by means of density gradient centrifugation and reduction with 2-mercaptoethanol (ME). It was found that the PHA was 10 to 100 times more sensitive than complement-fixation and neutralization tests for the detection of antibodies to adenovirus. The immunological response to primary immunization was dependent on the dose of antigen, with antibody appearing in as early as 3 days. After secondary stimulation with the same antigen, there was a rapid response which appeared to be less dose-dependent. It was found that a heavy 19S antibody sensitive to ME was produced early and was followed by a lighter, presumably 7S, ME-resistant antibody. Upon secondary stimulation, both 7S and 19S antibody increased to levels greater than those of the primary injection.
D000256:Adenoviridae / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D006386:Hemagglutination Tests; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D066298:In Vitro Techniques; D008623:Mercaptoethanol; D011817:Rabbits
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008623:Mercaptoethanol