Environmental temperature and choline requirements in rats. II. Choline and methionine requirements for lipotropic activity.
Young rats were fed choline-deficient diets and maintained at different environmental temperatures. The hepatic lipid level remained normal in rats at 2 degrees when 25 mg of choline per 100 g of food was fed; 50 mg of choline per 100 g food was required at 21 degrees and 100 mg of choline per 100 g food at 33 degrees to prevent excessive lipid accumulation. These values were equivalent to a mean daily intake per rat of 3 mg of choline at 2 degrees, 5.5 mg at 21 degrees, and 7 mg at 33 degrees respectively. When the growth rate was slower owing to a slight inadequacy of histidine in the basal choline-deficient diet, normal hepatic lipid was maintained by supplements of 50 mg of choline per 100 g food at 21 degrees and 33 degrees. Increasing the methionine content of the diet two- or three-fold from a basal value of 340 mg per 100 g food was as effective as 200 mg of choline per 100 g of food in lowering hepatic lipids at 2 degrees, 21 degrees, and 33 degrees.
D000818:Animals; D002794:Choline / Q000378:metabolism*; D002796:Choline Deficiency; D003677:Deficiency Diseases; D005234:Fatty Liver / Q000209:etiology*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D051381:Rats; D013696:Temperature
D008715:Methionine; D002794:Choline