Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones.
A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.
D000447:Aldehydes; D000818:Animals; D002855:Chromatography, Thin Layer; D004140:Dinitrophenols; D056890:Eukaryota; D066298:In Vitro Techniques; D008832:Microchemistry; D010659:Phenylhydrazines; D010714:Phosphatidylethanolamines; D010955:Plasmalogens; D013053:Spectrophotometry
D000447:Aldehydes; D004140:Dinitrophenols; D010659:Phenylhydrazines; D010714:Phosphatidylethanolamines; D010955:Plasmalogens