Prostaglandin: release from the rat phrenic nerve--diaphragm preparation.
Release of a substance that stimulates smooth muscle was detected from a phrenic nerve-diaphragm preparation from the rat upon direct and indirect stimulation. On thin-layer chromatography most of the active material behaved as a mixture of prostaglandins. The effect of electrical stimulation was mimicked by catecholamines but not by acetylcholine or eserine. The effect of nerve stimulation was not antagonized by d-tubocurarine.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001681:Biological Assay; D001920:Bradykinin / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003964:Diaphragm / Q000502:physiology; D004558:Electric Stimulation; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D009469:Neuromuscular Junction / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D010791:Phrenic Nerve / Q000502:physiology; D010830:Physostigmine / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000187:drug effects
D011453:Prostaglandins; D010830:Physostigmine; D000109:Acetylcholine; D001205:Ascorbic Acid; D001920:Bradykinin; D009638:Norepinephrine; D004837:Epinephrine