A respirometer for organ cultures.
1. A differential respirometer that can measure the oxygen uptake of organ cultures for periods of several days is described. 2. Diethanolamine is used as an external carbon dioxide buffer so that oxygen consumption can be measured in the presence of physiological concentrations of carbon dioxide and bicarbonate. 3. The efficiency of carbon dioxide retention with 5% carbon dioxide as gas phase is estimated to be 75% and the accuracy to be +/-5% with a measured rate of oxygen uptake in excess of 40mul./hr. 4. Some experiments with guinea-pig retina are reported.
D000818:Animals; D002021:Buffers; D002245:Carbon Dioxide; D046508:Culture Techniques; D006168:Guinea Pigs; D008365:Manometry / Q000295:instrumentation*; D010101:Oxygen Consumption; D012160:Retina / Q000378:metabolism
D002021:Buffers; D002245:Carbon Dioxide