Nuclear DNA and cytoplasmic DNA from tissues of higher plants.
Young wheat roots were labeled with (32)P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells.
D002467:Cell Nucleus; D055598:Chemical Phenomena; D002621:Chemistry; D002623:Chemistry Techniques, Analytical; D003593:Cytoplasm; D004247:DNA; D003720:Densitometry; D066298:In Vitro Techniques; D010759:Phosphorus Isotopes; D012639:Seeds; D014908:Triticum / Q000166:cytology*
D010759:Phosphorus Isotopes; D004247:DNA