The biosynthesis of raffinose.
1. Reaction of UTP and alpha-d-galactose 1-phosphate with [U-(14)C]sucrose in the presence of a Vicia faba dormant-seed preparation yielded the trisaccharide raffinose. 2. UTP-alpha-d-galactose 1-phosphate-uridylyltransferase activity has been demonstrated in the bean preparation and evidence for the participation of UDP-galactose in the trisaccharide synthesis is presented. 3. UDP-galactose 4-epimerase is present in the dormant seed. 4. The biosynthesis of raffinose in relation to the metabolism of other carbohydrates in plants is discussed.
D002854:Chromatography, Paper; D006600:Hexosephosphates / Q000378:metabolism*; D066298:In Vitro Techniques; D009713:Nucleotidyltransferases / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D010944:Plants / Q000201:enzymology; D014500:Uracil Nucleotides / Q000378:metabolism*
D006600:Hexosephosphates; D009844:Oligosaccharides; D014500:Uracil Nucleotides; D009713:Nucleotidyltransferases