Preservation of mammalian cells in a chemically defined medium and dimethylsulfoxide.
Three established mammalian cell lines (cat kidney, L, and HeLa cells), grown in suspension in a protein-free, chemically defined medium, were stored under liquid nitrogen in the defined medium fortified with 4 and 8 percent dimethylsulfoxide. After storage for 1 month the recovery viability was 86 percent, and cells showed normal growth upon reinoculation into the defined growth medium.
D000818:Animals; D002415:Cats; D002455:Cell Division; D046508:Culture Techniques; D004121:Dimethyl Sulfoxide; D006367:HeLa Cells; D007668:Kidney; D007739:L Cells; D009584:Nitrogen; D014015:Tissue Banks
D009584:Nitrogen; D004121:Dimethyl Sulfoxide