Transverse gradient electrophoresis: protein homogeneity test and subfractionation technique.
Electrophoresis of protein (including enzyme) was conducted in a gel medium across which, transverse to the direction of protein migration, a continuous (p)H gradient extends. Any splitting of a resultant trace for the change of protein mobility with (p)H suggests both protein heterogeneity and the (p)H conditions under which further purification and subfractionation may best be pursued.
D001797:Blood Protein Electrophoresis / Q000295:instrumentation; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation; D004586:Electrophoresis / Q000276:immunology* / Q000295:instrumentation*; D004798:Enzymes; D006801:Humans; D066298:In Vitro Techniques
D004798:Enzymes