Electrochromatography with reversing electrophoretic field.
A protein mixture can be separated in a flow column by application of a transverse electrophoretic field to carry faster-migrating components of the mixture into a fixed gel bed at one side of the column, so that slower components move along in a buffer stream. The field is then reversed to returnthe faster-moving components to the buffer stream. The cycle is repeated many times to complete the separation.
D002845:Chromatography; D004586:Electrophoresis