Alterations of protein synthesis in arbovirus-infected L cells.
Lust, George (Fort Detrick, Frederick, Md.). Alterations of protein synthesis in arbovirus-infected L cells. J. Bacteriol. 91:1612-1617. 1966.-Cellular protein synthesis and ribonucleic acid (RNA) synthesis in mouse L cells were markedly depressed 1 hr after infection with Venezuelan equine encephalomyelitis virus. Host RNA and protein synthesis were inhibited more rapidly by the virus infection than by actinomycin D. In cells infected 4 hr, a cytoplasmic RNA polymerase was demonstrated which was absent in uninfected cells. At this time, deoxyribonucleic acid-directed RNA synthesis catalyzed by the nuclear RNA polymerase was inhibited in vitro in enzyme preparations from nuclei of virus-infected cells. For optimal activity, the cytoplasmic RNA polymerase required the four nucleoside triphosphates, Mg(++), and RNA. The enzyme was insensitive to actinomycin D and deoxyribonuclease, indicating that it catalyzed RNA-directed RNA synthesis. Attempts to purify the induced polymerase further were unsuccessful. Fresh preparations had to be used because the enzymatic activity was unstable.
D000227:Adenine Nucleotides; D000818:Animals; D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D004247:DNA; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases; D004668:Encephalitis Viruses; D006150:Guanine Nucleotides; D066298:In Vitro Techniques; D007739:L Cells; D007930:Leucine / Q000378:metabolism; D008274:Magnesium; D051379:Mice; D009713:Nucleotidyltransferases; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D014500:Uracil Nucleotides
D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D014500:Uracil Nucleotides; D003609:Dactinomycin; D011691:Puromycin; D012313:RNA; D004247:DNA; D009713:Nucleotidyltransferases; D003851:Deoxyribonucleases; D007930:Leucine; D008274:Magnesium