Effects of alpha-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extracts.
White, David (Ames Research Center, Moffett Field, Calif.), and Harold P. Klein. Effects of alpha-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extracts. J. Bacteriol. 91:1218-1223. 1966.-The incorporation of acetate into fatty acids, but not into nonsaponifiable lipids, was stimulated by alpha-glycerophosphate in a supernatant fraction of Saccharomyces cerevisiae, obtained after centrifugation at 86,000 x g for 60 min. There was a pronounced effect at concentrations below 2 mm, but at concentrations above 5 mm alpha-glycerophosphate was relatively less stimulatory. alpha-Glycerophosphate markedly increased the percentage of esterified fatty acids among the products, and the formation of both saturated and unsaturated fatty acids was stimulated. Palmityl-coenzyme A inhibited fatty acid synthesis, affecting the formation of unsaturated acids more severely than saturated acids. In the presence of sufficient alpha-glycerophosphate to alleviate these inhibitions, palmityl-coenzyme A still reduced the formation of certain unsaturated fatty acids.
D000085:Acetates / Q000378:metabolism; D002498:Centrifugation; D002849:Chromatography, Gas; D003065:Coenzyme A / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis*; D005994:Glycerophosphates / Q000494:pharmacology*; D066298:In Vitro Techniques; D008055:Lipids / Q000096:biosynthesis*; D010169:Palmitic Acids / Q000494:pharmacology*; D012440:Saccharomyces / Q000378:metabolism*
D000085:Acetates; D005227:Fatty Acids; D005994:Glycerophosphates; D008055:Lipids; D010169:Palmitic Acids; D003065:Coenzyme A