Gamma-A cold agglutinin: importance of disulfide bonds in activity and structure.
Cleavage of disulfide bonds caused by mild reduction with mercaptoethanol produces a reversible loss of cold agglutinin activity and antigen-binding ability in a gamma-A polymer cold agglutinin. Return of agglutination and antigen-binding ability occur only in those molecules that have not been depolyimerized by reduction. Loss of activity becomes irreversible if reduction is followed immediately by alkylation.
D000577:Amides; D000906:Antibodies; D001205:Ascorbic Acid; D055598:Chemical Phenomena; D002621:Chemistry; D003080:Cold Temperature; D003553:Cystine; D005978:Glutathione; D007461:Iodoacetates; D008623:Mercaptoethanol; D013440:Sulfides
D000577:Amides; D000906:Antibodies; D007461:Iodoacetates; D013440:Sulfides; D003553:Cystine; D008623:Mercaptoethanol; D005978:Glutathione; D001205:Ascorbic Acid