Allomorphic forms of bacteriophage phiX-174 replicative DNA.
The bacteriophage øX-174 replicative form of DNA exists in two configurations, as judged by electron microscopy and sucrose density gradient sedimentation, and on methylated albumin-coated kieselguhr column chromatography. The predominant 21S form of the DNA is a tightly twisted circle. Treatment with deoxyribonuclease causes a single scission in one of the two strands, resulting in an open circle which sediments at 16S.
D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D004279:DNA, Viral / Q000032:analysis*; D003851:Deoxyribonucleases; D008854:Microscopy, Electron; D014461:Ultracentrifugation
D004279:DNA, Viral; D003851:Deoxyribonucleases