Coding properties of 7-methylguanine.
The abnormal base, 7-methylguanine, has been introduced into copolymers of uridylic and guanylic acids by methylation under controlled conditions. The resulting methylated copolymers have a markedly decreased template activity for polypeptide synthesis in vitro due to steric effects of the 7-methyl groups. Contrary to expectation, these polymers do not permit incorporation of amino acids whose codons contain the bases uracil and adenine.
D000596:Amino Acids; D002845:Chromatography; D006147:Guanine; D006150:Guanine Nucleotides; D006863:Hydrogen-Ion Concentration; D009153:Mutagens; D011108:Polymers; D014461:Ultracentrifugation; D014500:Uracil Nucleotides
D000596:Amino Acids; D006150:Guanine Nucleotides; D009153:Mutagens; D011108:Polymers; D014500:Uracil Nucleotides; D006147:Guanine