Influenza virus purification with the zonal ultracentrifuge.
Sufficient amounts of influenza virus (density, 1.185; size, 722S) can be highly purified (22,000 chicken cell agglutinating units per milligram of protein) with a zonal ultracentrifuge, used first in a rate process followed by isopycnic banding, to permit its detailed biological and physical-chemical evaluation.
D000818:Animals; D006386:Hemagglutination Tests; D066298:In Vitro Techniques; D051379:Mice; D008854:Microscopy, Electron; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D011817:Rabbits; D014461:Ultracentrifugation