Cytotoxic effects in vitro of highly purified streptolysin O on mouse macrophages cultured in a serum-free medium.
Fauve, Robert M. (Institut Pasteur, Garches (Hauts de Seine), France), Joseph E. Alouf, Albert Delaunay, and Marcel Raynaud. Cytotoxic effects in vitro of highly purified streptolysin O on mouse macrophages cultured in a serum-free medium. J. Bacteriol. 92:1150-1153. 1966.-After the addition of highly purified streptolysin O to mouse macrophages cultured in medium 199 containing albumin, the following cellular alterations were observed. The disappearance of the stellate shape of the cells and the formation of bleb-like vesicles extruding from the cytoplasmic membrane occurred within a few minutes. Later, massive degranulation and formation of cytoplasmic vesicles were followed by cell death.
D000818:Animals; D003470:Culture Media; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D013301:Streptolysins / Q000494:pharmacology*
D003470:Culture Media; D013301:Streptolysins