Distribution of C-14 from glucose-1-C-14 in the lipid fractions of Debaryomyces hansenii.
Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Rosalind H. Frye. Distribution of C(14) from glucose-1-C(14) in the lipid fractions of Debaryomyces hansenii. J. Bacteriol. 91:1831-1833. 1966.-Debaryomyces hansenii cells were grown in a medium containing yeast extract, malt extract, glucose, sodium chloride, and nutrient salts, to which glucose-1-C(14) was added. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total C(14) added to the culture medium, the neutral lipid fractions contained 21.06% while the phospholipid portions contained only 0.89%. The highest amount of C(14) among the neutral lipids was found in the fraction containing the hydrocarbons (11.64%). Among the phospholipids, the highest amount (0.66%) was found in phosphatidyl serine and phosphatidyl ethanolamine.
D002247:Carbon Isotopes; D002845:Chromatography; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D012440:Saccharomyces / Q000378:metabolism*
D002247:Carbon Isotopes; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D005947:Glucose