Dealkylation and loss of capacity for reactivation of cholinesterase inhibited by sarin.
Inhibition of rat brain acetylcholinesterase by (32)P-sarin in vivo results initially in (32)P-isopropylmethylphosphonylated enzyme. The percentage of inhibited enzyme that could not be reactivated by pyridinium aldoxime methochloride (aged enzyme) approximated the amount of radioactivity identified as (32)P-methylphosphonate. The (32)P-isopropyl methylphosphonate not released fromthe inhibited enzyme by the oxime accounted for 51 +/- 10 percent ( standard deviation) of the radioactivity fixed to brain tissue. It showed no correlation with aging and was probably bound to sites other than acetylcholinesterase.
D000375:Aging; D000818:Animals; D001923:Brain Chemistry; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002802:Cholinesterases; D066298:In Vitro Techniques; D010091:Oximes / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D011874:Radiometry; D051381:Rats
D002800:Cholinesterase Inhibitors; D010091:Oximes; D010710:Phosphates; D002802:Cholinesterases