Effects of Triton-X-100 upon the mobility of esterases and alkaline phosphatases in disc electrophoresis.
Homogenates of the mid gut and digestive glands of Rhynchosciara angelae were prepared in 40% sucrose, with and without 0.5% Triton-X-100. These were subjected to disc electrophoresis and zymograms for esterases and alkaline phosphatase were prepared. In both groups the effect of Triton-X-100 was to increase the electrophoretic mobility of the enzymes concerned.
D000469:Alkaline Phosphatase / Q000032:analysis*; D004175:Diptera; D004586:Electrophoresis; D004950:Esterases / Q000032:analysis*; D007422:Intestines / Q000201:enzymology; D013501:Surface-Active Agents / Q000494:pharmacology*
D013501:Surface-Active Agents; D004950:Esterases; D000469:Alkaline Phosphatase