Inhibitory proteins in the Newcastle disease virus-induced suppression of cell protein synthesis.
Bolognesi, D. P. (Rensselaer Polytechnic Institute, Troy, N.Y.), and D. E. Wilson. Inhibitory proteins in the Newcastle disease virus-induced suppression of cell protein synthesis. J. Bacteriol. 91:1896-1901. 1966.-Infection by Newcastle disease virus brings about a rapid and marked inhibition of cell protein synthesis (CPS) in chick embryo fibroblast monolayers. The block to CPS is initiated about 5 hr after infection, and by 9 hr about 85% of the host protein synthesis is shut off. Azauridine (3 mg/ml), a ribonucleic acid (RNA) synthesis inhibitor, prevents the virus-induced inhibition of CPS when added at the time of infection; but it does not prevent the inhibition when added at 3 hr after infection. When puromycin (60 mug/ml), a protein synthesis inhibitor, was added at 3.5 hr after infection, viral RNA was synthesized in normal amounts, but the virus-induced inhibition of CPS was prevented. Actinomycin D added at the time of infection does not, however, prevent the virus-induced inhibition of CPS. The results of these experiments indicate that proteins synthesized during Newcastle disease virus replication are responsible for the inhibition of host-cell protein synthesis. The synthesis of these inhibitory proteins depends on the prior synthesis of viral RNA.
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D002247:Carbon Isotopes; D002642:Chick Embryo; D003609:Dactinomycin / Q000494:pharmacology; D004798:Enzymes; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D009521:Newcastle Disease; D009522:Newcastle disease virus / Q000254:growth & development*; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D001391:Azo Compounds; D002247:Carbon Isotopes; D004798:Enzymes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D011691:Puromycin; D007930:Leucine; D014529:Uridine