Antibody formation in nonimmune mouse peritoneal cells after incubation in gum containing antigen.
Peritoneal cells from normal mice in a semisolid medium containing sheep erythrocytes were incubated at 37 degrees C for 2 or 3 days. During this period, hemolytic antibodies developed spontaneously. Arguments are presented that true de novo synthesis of antibody has taken place in previously uncommitted cells.
D000818:Animals; D000917:Antibody Formation; D004912:Erythrocytes; D066298:In Vitro Techniques; D008198:Lymph Nodes; D008747:Methylcellulose; D051379:Mice; D010537:Peritoneum; D011817:Rabbits; D012116:Resins, Plant; D012756:Sheep; D013154:Spleen
D012116:Resins, Plant; D008747:Methylcellulose