Transformation of auxotrophic mutants of group H streptococci.
Stable auxotrophic mutants of a group H streptococcus (strain Challis) were isolated on a modified Mickelson defined medium after exposure to N-methyl- N-nitro-N-nitrosoguanidine. Such mutants were transformed to both prototrophy and streptomycin-resistance and hence may be used as nutritional markers in the study of streptococcal genetics.
D003470:Culture Media; D004352:Drug Resistance, Microbial; D005823:Genetics; D006146:Guanidines / Q000494:pharmacology; D066298:In Vitro Techniques; D009154:Mutation; D009603:Nitroso Compounds / Q000494:pharmacology; D013291:Streptococcus; D013307:Streptomycin / Q000494:pharmacology*
D003470:Culture Media; D006146:Guanidines; D009603:Nitroso Compounds; D013307:Streptomycin