Lipid films as transducers for detection of antigen-antibody and enzyme-substrate reactions.
The transverse electrical impedance of thin lipid films separating two aqueous saline solutions containing a small concentration of antibodies or enzymes decreases markedly and reversibly after immunological and enzymatic reactions involving such protein molecules, which presumably are adsorbed to the lipid-water interface.
D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D002621:Chemistry; D002802:Cholinesterases; D002918:Chymotrypsin; D004798:Enzymes; D005969:Glutamate Dehydrogenase; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase; D008055:Lipids; D010047:Ovalbumin; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D014357:Trypsin; D014503:Urate Oxidase; D014510:Urease
D004798:Enzymes; D008055:Lipids; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D014503:Urate Oxidase; D002802:Cholinesterases; D002918:Chymotrypsin; D014357:Trypsin; D014510:Urease