Pyruvate-carbon dioxide exchange reaction of Desulfovibrio desulfuricans.
Suh, Byungse (University of Kansas, Lawrence), and J. M. Akagi. Pyruvate-carbon dioxide exchange reaction of Desulfovibrio desulfuricans. J. Bacteriol. 91:2281-2285. 1966.-The pyruvate-CO(2) exchange reaction, catalyzed by Desulfovibrio desulfuricans, required the presence of phosphate and coenzyme A. However, the requirement for phosphate disappeared when the concentration of coenzyme A was increased to a level of 3.8 x 10(-3)m. Passing crude extracts through a diethylaminoethyl-cellulose column and an Amberlite CG-50 ion-exchange column, to remove ferredoxin and cytochrome c(3), resulted in a marked decrease in exchange activity; full activity was restored by the addition of ferredoxin or cytochrome c(3). Fe(++) or Co(++) stimulated the exchange of CO(2) into pyruvate.
D002245:Carbon Dioxide / Q000378:metabolism*; D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D003065:Coenzyme A; D003580:Cytochromes / Q000378:metabolism; D003901:Desulfovibrio / Q000378:metabolism*; D004579:Electron Transport; D005288:Ferredoxins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010710:Phosphates; D011773:Pyruvates / Q000378:metabolism*
D002247:Carbon Isotopes; D003580:Cytochromes; D005288:Ferredoxins; D010710:Phosphates; D011773:Pyruvates; D002245:Carbon Dioxide; D003065:Coenzyme A