End-organ effects of thyroid hormones: subcellular interactions in cultured cells.
Both actinomycin D and puromycin suppress the formation of colonies by cultured human kidney epithelial cells (T-l), but inactivation by puromycin is partially reversed with thyroid hormones. Uptake by the cells of L-thyroxine labeled with iodine-125, 60 to 80 percent of which is nuclear, is depressed by actinomycin and enhanced by puromycin. Genome and possibly nuclear membrane are implicated as initiating loci.
D001345:Autoradiography; D046508:Culture Techniques; D004247:DNA; D004848:Epithelium / Q000187:drug effects*; D006801:Humans; D007456:Iodine Isotopes; D007668:Kidney; D011691:Puromycin / Q000494:pharmacology*; D012333:RNA, Messenger; D013974:Thyroxine / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology*
D007456:Iodine Isotopes; D012333:RNA, Messenger; D014284:Triiodothyronine; D011691:Puromycin; D004247:DNA; D013974:Thyroxine