Complete separation of lipid classes on a single thin-layer plate.
A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were separated from cholesterol, 1,2- from 1,3-diglycerides, and monoglycerides from phospholipids.
D000085:Acetates; D000473:Alkanes; D001554:Benzene; D002784:Cholesterol; D002855:Chromatography, Thin Layer; D004987:Ethers; D005989:Glycerides; D008055:Lipids; D010743:Phospholipids; D012997:Solvents
D000085:Acetates; D000473:Alkanes; D004987:Ethers; D005989:Glycerides; D008055:Lipids; D010743:Phospholipids; D012997:Solvents; D002784:Cholesterol; D001554:Benzene