Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.
1. The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D005696:Galactosidases / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D012756:Sheep; D013737:Testis / Q000201:enzymology*
D005696:Galactosidases; D006026:Glycoside Hydrolases