Influence of cobalt on fermentative methylation.
Streptomyces rishiriensis produces at least five closely related antibiotics. Strain selection yielded a culture producing only the most active component, coumermycin A. Hydrolysis of this antibiotic by barium hydroxide yielded both 5-methyl-pyrrole-2-carboxylic acid and pyrrole-2-carboxylic acid, which could be separated by paper chromatography. Coumermycin A was thus shown to be two fractions, designated A(1) and A(2) depending upon the nature of the pyrrole carboxylic acid portion. The addition of cobalt to the fermentation medium at a level as low as 0.01 mug/ml shifted the fermentation exclusively to the production of coumermycin A(1). Other ions were ineffective, except nickel, whose activity could be explained by the presence of contaminating cobalt.
D000478:Alkylation; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D001464:Barium; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D003035:Cobalt / Q000494:pharmacology*; D006878:Hydroxides; D009532:Nickel / Q000494:pharmacology; D013053:Spectrophotometry; D013302:Streptomyces / Q000378:metabolism*; D014805:Vitamin B 12 / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D006878:Hydroxides; D001464:Barium; D003035:Cobalt; D009532:Nickel; D014805:Vitamin B 12