Lysosomal hydrolases in calf thyroid.
1. Two acid phosphatases (beta-glycerophosphatase and phenylphosphatase), acid beta-glucuronidase and cathepsin were demonstrated in the 0.25m-sucrose homogenates from whole calf thyroid tissue and from isolated calf thyroid cells. 2. The main kinetic characters of these enzymes were studied. 3. All these acid hydrolases are partially sedimentable and display a latency that is unmasked by treatment with Triton X-100 and on dilution in hypo-osmotic media. It is concluded that these acid hydrolases belong to the lysosomes.
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D002403:Cathepsins / Q000032:analysis*; D002417:Cattle; D005615:Freezing; D005966:Glucuronidase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013395:Sucrose / Q000494:pharmacology; D013961:Thyroid Gland / Q000201:enzymology*
D013395:Sucrose; D000135:Acid Phosphatase; D005966:Glucuronidase; D002403:Cathepsins