Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro.
1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in the medium. 3. Heparin, glucose, insulin, and HCO(3) (-) and K(+) ions are shown to be important medium constituents.
D000273:Adipose Tissue / Q000201:enzymology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008074:Lipoproteins / Q000097:blood; D051381:Rats; D013217:Starvation
D000596:Amino Acids; D001639:Bicarbonates; D003470:Culture Media; D007328:Insulin; D008074:Lipoproteins; D006493:Heparin; D008071:Lipoprotein Lipase; D005947:Glucose