Nutrition and metabolism of marine bacteria. XV. Relation of Na+-activated transport to the Na+ requirement of a marine pseudomonad for growth.
Drapeau, Gabriel R., (McGill University, Montreal, Quebec, Canada), Tibor I. Matula, and Robert A. MacLeod. Nutrition and metabolism of marine bacteria. XV. Relation of Na(+)-activated transport to the Na(+) requirement of a marine pseudomonad for growth. J. Bacteriol. 92:63-71. 1966.-A marine pseudomonad was found to require 50 to 100 mm Na(+) for maximal rate of oxidation of d-galactose and for the transport of d-fucose-H(3) into the cells. The same organism required 150 to 200 mm Na(+) for the oxidation of l-alanine and for the transport of phi-aminoisobutyric acid-C(14) (AIB-C(14)) into the cells. Competition studies indicated that d-galactose and d-fucose on the one hand and l-alanine and AIB on the other shared common carriers for transporting the compounds into the cells. This parallelism in Na(+) response for oxidation and transport extended to growth when l-alanine was the sole carbon source in the medium. When d-galactose was the sole carbon source, an amount of Na(+) equal to that with l-alanine was needed. KCN and dinitrophenol but not ouabain inhibited the uptake of AIB-C(14) by the cells. K(+) in addition to Na(+) was required for transport, and both Mg(++) and either Cl(-) or Br(-) were stimulatory. Photobacterium fischeri was also found to require Na(+) specifically for the uptake of AIB-C(14) by the cells.
D000409:Alanine / Q000378:metabolism; D000613:Aminobutyrates / Q000378:metabolism*; D001693:Biological Transport, Active; D001965:Bromides / Q000494:pharmacology; D002247:Carbon Isotopes; D002712:Chlorides / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D066298:In Vitro Techniques; D008274:Magnesium; D008386:Marine Biology; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011549:Pseudomonas / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D014316:Tritium
D000613:Aminobutyrates; D001965:Bromides; D002247:Carbon Isotopes; D002712:Chlorides; D003486:Cyanides; D004140:Dinitrophenols; D014316:Tritium; D005643:Fucose; D010042:Ouabain; D012964:Sodium; D008274:Magnesium; D000409:Alanine; D011188:Potassium; D005690:Galactose