Serum high-density lipoprotein: effect of change in structure on activity of chicken adipose tissue lipase.
The high-density (1.063 to 1.21 g/ml) lipoprotein in human serum was analyzed as activator for a lipoprotein lipase isolated from chicken adipose tissue. The activating capacity was lost when the lipoprotein was extracted with a mixture of ethanol and ethyl ether (3:2 by volume) at -10 degrees C and it was restored upon incubation of the extracted protein with aqueous sols of either whole phospholipids or the lecithin fraction prepared from the high-density lipoprotein. Since phospholipid sols alone proved ineffective as substrate activators, the complex which forms upon incubation of the extracted lipoprotein with phospholipids appears to be a necessary requirement for lipoprotein lipase activity.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D001769:Blood; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism*; D008074:Lipoproteins; D011200:Poultry
D008074:Lipoproteins; D008049:Lipase