Inhibitory effect of salicylate on the incorporation of L-(U-14C)leucine into the protein of rat tissue preparations in vitro.
1. The inhibitory effect of salicylate, in concentrations ranging from 0.1 to 20mm, on the incorporation of radioactivity from l-[U-(14)C]leucine into the protein of isolated rat diaphragm muscle and of cell-free systems from rat liver was studied. 2. The lowest salicylate concentrations producing significant inhibitions of amino acid incorporation were as follows: isolated rat diaphragm, 0.1mm; rat-liver mitochondrial-microsomal system, 0.1mm; rat-liver microsomal system, 0.3mm. 3. Salicylate concentrations of 2.5mm and above were found to inhibit creatine-kinase activity in vitro.
D000818:Animals; D002247:Carbon Isotopes; D003402:Creatine Kinase / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D012459:Salicylates / Q000494:pharmacology*
D002247:Carbon Isotopes; D012459:Salicylates; D003402:Creatine Kinase; D007930:Leucine