Morphogenesis in Trichoderma: suppression of photoinduction by 5-fluorouracil.
Sporulation in the fungus Trichoderma viride is inducible with a short light pulse. 5-Fluorouracil applied prior to photoinduction and removed thereafter suppressed sporulation without greatly aflecting growth. This compound also halved the rate of incorporation of uracil-C(14) into RNA but did not change the ratio of uridylic to cytidylic acid. The effect of 5-fluorouracil was counteracted by uracil but not by thymidine. This supports the hypothesis that 5-fluorouracil affects RNA rather than DNA.
D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D004247:DNA; D005472:Fluorouracil / Q000494:pharmacology*; D066298:In Vitro Techniques; D003904:Mitosporic Fungi / Q000187:drug effects* / Q000254:growth & development*; D012313:RNA / Q000096:biosynthesis*; D011874:Radiometry; D013170:Spores; D013936:Thymidine; D014498:Uracil; D014500:Uracil Nucleotides
D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D014500:Uracil Nucleotides; D014498:Uracil; D012313:RNA; D004247:DNA; D005472:Fluorouracil; D013936:Thymidine