Modified agglutination test for Pasteurella tularensis.
Several modifications in technique were incorporated into the standard agglutination test for Pasteurella tularensis. Reciprocal shaking of all tubes in a Kahn shaker was introduced to increase the rate of agglutination and quantity of agglutinated cell mass, making it possible to report preliminary results within 4 hr. Increased incubation time at a higher temperature was used to favor the rate of agglutination. A serum control for each serum tested was necessary to detect false positive tests. Finally, a verification procedure with 5% NaCl used as the diluent was instituted to prevent these false positive reactions.
D000372:Agglutination Tests; D005604:Francisella tularensis; D012965:Sodium Chloride; D013696:Temperature; D014406:Tularemia / Q000175:diagnosis*
D012965:Sodium Chloride