The enzymic estimation of glutamate and glutamine.
A method of estimating glutamic acid is described, based on its dehydrogenation by glutamate dehydrogenase coupled, by means of N-methylphenazine methosulphate, to the reduction of tetrazolium salts. The method is suitable for the estimation of 0-0.3mumole of glutamic acid. The response is linear, but not stoicheiometric: possible reasons for this are discussed. If suitable precautions are taken, the use of a partially purified preparation of glutaminase makes it possible to estimate glutamine also.
D055598:Chemical Phenomena; D002621:Chemistry; D003486:Cyanides; D005969:Glutamate Dehydrogenase; D005971:Glutamates / Q000032:analysis*; D005972:Glutaminase; D005973:Glutamine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen; D010619:Phenazines; D013053:Spectrophotometry; D013696:Temperature; D013778:Tetrazolium Salts
D003486:Cyanides; D005971:Glutamates; D010619:Phenazines; D013778:Tetrazolium Salts; D005973:Glutamine; D005969:Glutamate Dehydrogenase; D005972:Glutaminase; D010100:Oxygen