The antigen-binding capacity of the peptide chains of horse antibodies.
The antigen-binding capacity of the peptide chains of horse anti-(diphtheria toxin) has been studied by using (125)I-labelled toxoid and electrophoresis of antibody-antigen mixtures on cellulose acetate. The heavy chains retained about 20% of the activity of the whole antibody and the light chains less than 5%. Recombination of specific heavy and light chains gave about 60% recovery of activity and recombination of specific heavy chains and non-specific light chains about 40% recovery. It is suggested that these results favour the heavy chain as the major site of the antigen-binding activity.
D000818:Animals; D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D002621:Chemistry; D003013:Clostridium / Q000201:enzymology; D004167:Diphtheria Toxin; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007456:Iodine Isotopes; D010455:Peptides; D010740:Phospholipases; D013745:Tetanus Toxoid
D004167:Diphtheria Toxin; D007106:Immune Sera; D007456:Iodine Isotopes; D010455:Peptides; D013745:Tetanus Toxoid; D010740:Phospholipases