Trehalase from Dictyostelium discoideum: purification and properties.
The hydrolytic enzyme, trehalase, was isolated and purified approximately 90-fold from the cellular slime mold Dictyostelium discoideum. The purified trehalase has an optimal temperature of 45 degrees C and shows maximum activity at pH 5.5 in citrate buffer. Its Michaelis constant is 1.2x 10(-3)M.
D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002623:Chemistry Techniques, Analytical; D004187:Disaccharides; D006026:Glycoside Hydrolases; D009235:Myxomycetes / Q000201:enzymology*
D004187:Disaccharides; D006026:Glycoside Hydrolases