Components of the cell wall of Clostridium welchii (type A).
1. The cell wall of Clostridium welchii (type A) contains alanine, 2,6-diaminopimelic acid, glutamic acid, glycine, glucosamine, muramic acid, galactosamine, mannosamine, ethanolamine, rhamnose, galactose and phosphorus. 2. Heating with formamide at 150 degrees resolved the wall into a formamide-soluble polysaccharide fraction and a formamide-insoluble mucopeptide fraction. 3. The formamide-soluble fraction contained two components: an electrophoretically neutral polysaccharide made up of galactose, rhamnose, galactosamine and phosphorus and an electrophoretically acidic polymer containing mannosamine, ethanolamine and phosphorus. 4. The formamide-insoluble residue has been digested by lysozyme to give soluble fragments of high molecular weight. 5. All fractions contain an unknown ethyl acetate-extractable substance that can be oxidized by sodium metaperiodate. 6. The amino acid compositions of the fragments produced by lysozyme are compatible with a mucopeptide structure which has cross bridges containing all of the constituent amino acids.
D000596:Amino Acids / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002473:Cell Wall / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003013:Clostridium / Q000166:cytology*; D004586:Electrophoresis; D006595:Hexosamines / Q000032:analysis*; D010429:Pentoses / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D006595:Hexosamines; D010429:Pentoses; D011134:Polysaccharides; D010758:Phosphorus