The localization of lysosomal enzymes in chromaffin tissue.
1. An homogenate of bovine adrenal medulla contains significant amounts of six acid hydrolases: acid ribonuclease, acid deoxyribonuclease, cathepsin, acid phosphatase, beta-glucuronidase and arylsulphatase. Most of the activity of each enzyme could be sedimented in the large-granule fraction at 242,000 g-min.2. Differential centrifugation indicated the presence of three populations of particles, which sedimented at slightly different rates; these are, in order of decreasing sedimentation rate, mitochondria, particles containing the acid hydrolases, and chromaffin granules.3. The three types of particle could be separated by ultracentrifuging the large-granule fraction in a sucrose density gradient. Most of the activity of each hydrolase was recovered in a layer intermediate between those formed by mitochondria and chromaffin granules.4. The large-granule fraction therefore contains particles which are defined by their enzyme content as lysosomes.5. Highly purified chromaffin granules, containing less than 5% of the activity of each acid hydrolase, were obtained from the gradient.
D000135:Acid Phosphatase / Q000378:metabolism*; D000313:Adrenal Medulla / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002403:Cathepsins / Q000378:metabolism*; D002417:Cattle; D002498:Centrifugation; D002838:Chromaffin System / Q000201:enzymology*; D003851:Deoxyribonucleases / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism*; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology*; D012260:Ribonucleases / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013429:Sulfatases / Q000378:metabolism*
D003851:Deoxyribonucleases; D012260:Ribonucleases; D000135:Acid Phosphatase; D013429:Sulfatases; D005966:Glucuronidase; D002403:Cathepsins