The effect of castration on the ribonucleic acid metabolism of an experimental prostatic tumour.
1. The incorporation of [(14)C]phenylalanine into the protein of microsomes obtained from an androgen-dependent transplantable prostatic tumour has been investigated. 2. The incorporation of [(14)C]ATP into the RNA of the nuclei from the same tumour has also been examined. 3. The castration of the host animal depresses the incorporation of both labelled compounds on subsequent incubation in vitro. 4. The uptake of phenylalanine can be markedly stimulated by polyuridylic acid only in tumours obtained from castrated host animals. 5. The tumour RNA-polymerase activity appears to be dependent on the concentrations of androgens in the host animal.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D002369:Castration; D002467:Cell Nucleus / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D051381:Rats
D001551:Benz(a)Anthracenes; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D010649:Phenylalanine; D000255:Adenosine Triphosphate; D009713:Nucleotidyltransferases