The fate of acetyl groups derived from glucose in the isolated perfused goat udder.
1. In the isolated perfused goat mammary gland, both glucose and acetate contribute 20-30% of carbon to citrate via acetyl-CoA. 2. Carbon dioxide does not contribute significantly to the formation of acetyl-CoA. 3. ATP citrate lyase, which, in rats, yields acetyl-CoA for fatty acid synthesis, is absent from or very low in high-speed supernatant fraction of ruminant tissue. 4. It is reasonable to infer that intramitochondrial acetyl-CoA, to which both glucose and acetate contribute, is separated from extramitochondrial acetyl-CoA to which glucose does not contribute and which is used for fatty acid synthesis.
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D002951:Citrates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D006041:Goats; D008190:Lyases / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*
D000085:Acetates; D002247:Carbon Isotopes; D002951:Citrates; D005971:Glutamates; D008190:Lyases; D005947:Glucose