A comparative study in vivo and in vitro of the ability of ribosomes from Xenopus liver and ovary to incorporate L-[U-14C]leucine.
1. A system for the incorporation in vitro of amino acids into protein is described for the South African clawed toad (Xenopus laevis laevis Daudin). 2. The incorporation of l-[U-(14)C]leucine by Xenopus-liver microsomes is very much greater per mg. of microsomal RNA than the incorporation by ovary microsomes. 3. The incorporation by Xenopus-liver and -ovary polysomes is approximately the same when expressed per mg. of polysomal RNA. 4. It was predicted from the above results that ovary microsomes should contain a ribosomal fraction inactive in protein synthesis. This was shown to be the case by a labelling experiment in vivo with l-[U-(14)C]leucine. 5. The labelling experiment in vivo also showed that the active polysomal fraction in ovary is associated with membranes and is liberated by treatment with deoxycholate; this is also true of liver microsomes in vivo. 6. The results are discussed in relation to previous work on the synthesis of proteins by amphibian ovarian tissue, and on the role of bound and free ribosomes in protein synthesis.
D000818:Animals; D001001:Anura; D002499:Centrifugation, Density Gradient; D005260:Female; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism*
D012313:RNA; D007930:Leucine