Amino acid incorporation by preparations from the developing rat brain.
1. A study has been made of the ability of cerebral microsome-cell sap systems, taken from rats at various ages, to incorporate [(14)C]valine. 2. The systems appear to follow Michaelis-Menten kinetics when cell sap is considered as substrate, microsomes as enzyme and total counts/mg. of microsomal protein as a measure of reaction velocity. 3. Reproducible ;affinity constant' values are obtained, and the system from 4-day-old rats has a higher V(max.) and affinity constant than the system from adult rats. 4. It is suggested the the amino acid-incorporating ability of different systems may be compared by this means.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014633:Valine / Q000378:metabolism*
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D014633:Valine