Multiple alterations in metabolite uptake in a mutant of Neurospora crassa.
A temperature-conditional lethal mutant of Neurospora crassa, un-t (55701), was resistant to neutral amino acid analogues by virtue of a decreased ability to transport these analogues and their natural congeners across the cell membrane. The uptake of acidic, but not basic, amino acids was also impaired, as was the uptake of potassium ions. After preincubation above the tolerated temperature, the ability to take up a still wider variety of metabolites was greatly reduced. Protoplasts of the mutant were more osmotically fragile than those of wild type. The possibility that the mutant has a generalized membrane defect is discussed.
D000085:Acetates / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D002247:Carbon Isotopes; D002462:Cell Membrane; D002463:Cell Membrane Permeability; D003513:Cycloheximide / Q000494:pharmacology; D005001:Ethionine / Q000378:metabolism; D005461:Fluorine; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D009491:Neurospora / Q000254:growth & development / Q000378:metabolism*; D009996:Osmotic Fragility; D010649:Phenylalanine / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011194:Potassium Isotopes; D014176:Protein Biosynthesis; D011523:Protoplasts; D013431:Sulfates / Q000378:metabolism; D013460:Sulfur Isotopes; D013696:Temperature
D000085:Acetates; D000596:Amino Acids; D002247:Carbon Isotopes; D010710:Phosphates; D011194:Potassium Isotopes; D013431:Sulfates; D013460:Sulfur Isotopes; D005461:Fluorine; D010649:Phenylalanine; D003513:Cycloheximide; D005947:Glucose; D011188:Potassium; D005001:Ethionine