Organic acid production by Basidiomycetes. 3. Cultural conditions for L-malic acid production.
Cultural conditions were examined for the purpose of increasing yields of l-malic acid by the Basidiomycetes Schizophyllum commune and Merulius tremellosus, which have the ability to produce this acid as a main product in CaCO(3)-containing medium in shaken culture. The most favorable nitrogen sources selected were 0.3% (NH(4))(2)SO(4) and 0.18% NH(4)Cl. Effective combinations of inorganic salts in the medium were 0.1% KH(2)PO(4), 0.05% MgSO(4).7H(2)O, and 0.05% KCl, and suitable concentrations of glucose were 5 to 10%. Several nonionic surface-active agents promoted the filamentous mycelial growth of these strains and increased acid production. In particular, Tween 80 in 0.3% concentration markedly stimulated malic acid production by S. commune, and yields greater than 50% based on available glucose, were obtained after 10 to 14 days. Acid production by M. tremellosus was stimulated most with 0.5% Carbowax 4000 (polyethylene glycol), and the resultant yields were more than 40%.
D001487:Basidiomycota / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D008293:Malates / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology; D010461:Peptones / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D003470:Culture Media; D008293:Malates; D009566:Nitrates; D010461:Peptones; D000644:Quaternary Ammonium Compounds; D014508:Urea; D005947:Glucose