Electrical phenomena associated with the activity of the membrane-bound acetylcholinesterase.
Treatment of isolated electroplax with physiological solutions supplemented with either 1 molar sodium chloride, 2 molar urea, or 2 molar sucrose renders the cell insensitive to carbamylcholine, phenyltrimethylammonium, or decamethonium even at high concentrations. The treated cells have a residual resting potential of -20 +/- 10 millivolts (negative inside) and are depolarized by acetylcholine at concentrations larger than 10(-3) mole per liter. This response is not affected by d-tubocurarine but is blocked by physostigmine, diisopropylphosphorofluoridate, or strong buffers and thus depends on the catalytic activity of the membrane-bound acetylcholinesterase.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002384:Catalysis; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology*; D003650:Decamethonium Compounds / Q000494:pharmacology; D004524:Eels; D004557:Electric Organ / Q000201:enzymology; D004594:Electrophysiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology; D008564:Membrane Potentials; D009132:Muscles / Q000201:enzymology*; D010830:Physostigmine / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D014403:Tubocurarine / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D003650:Decamethonium Compounds; D000644:Quaternary Ammonium Compounds; D007531:Isoflurophate; D012965:Sodium Chloride; D013395:Sucrose; D014508:Urea; D002217:Carbachol; D010830:Physostigmine; D000110:Acetylcholinesterase; D014403:Tubocurarine