Distribution of radioactive glycerol and fatty acids among adipose tissue triglycerides after administration of glucose-U-14C.
Adipose lipid obtained from fed rats 15 or 60 min after injection of radioactive glucose was separated into 10 triglyceride classes of differing fatty acid compositions. The distribution among these classes of total and radioactive triglyceride-glycerol was determined and found to be the same. Thus newly synthesized adipose triglycerides resemble in kind and proportion the triglycerides which exist in the tissue. This finding is in accord with the concept that the structures of adipose triglycerides are stable over long periods and that the turnover rate of the several triglyceride species are similar. After administration of radioactive glucose, the specific activity of saturated fatty acids was higher in the more saturated triglyceride species. These data indicate that newly formed saturated acids do not mix completely with all adipose tissue fatty acids available for esterification. Fatty acids derived from plasma triglyceride influenced the composition of newly synthesized adipose tissue triglyceride and thus constitute an important source of adipose tissue lipid.
D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D002247:Carbon Isotopes; D002855:Chromatography, Thin Layer; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis*; D005947:Glucose / Q000378:metabolism*; D005989:Glycerides / Q000032:analysis*; D005990:Glycerol / Q000032:analysis; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000032:analysis
D002247:Carbon Isotopes; D004041:Dietary Fats; D005227:Fatty Acids; D005989:Glycerides; D014280:Triglycerides; D005947:Glucose; D005990:Glycerol