A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids.
1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring amino acids tested did not give a similar reaction. 3. The reaction product was stable for at least 3-4hr. at room temperature and the extinction was proportional to the concentration in the range 0.05-0.5mumole of cysteine. 4. The acid ninhydrin reagent also gave yellow products (E(max.) 370-404mmu) with tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine and indol-3-ylacetic acid. 5. The method was applied for the determination of cysteine in perchloric acid extracts of rat brain, liver and blood.
D000596:Amino Acids / Q000032:analysis*; D001774:Blood Chemical Analysis; D001923:Brain Chemistry; D003545:Cysteine / Q000032:analysis*; D006851:Hydrochloric Acid / Q000494:pharmacology; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D013053:Spectrophotometry
D000596:Amino Acids; D007202:Indicators and Reagents; D003545:Cysteine; D006851:Hydrochloric Acid