Schwann cell proliferation in developing mouse sciatic nerve. A radioautographic study.
Proliferation of Schwann cells in neonatal mouse sciatic nerve was studied radioautographically in 1-micro glycol methacrylate sections. 28 mice were injected with thymidine-(3)H, 4 microc/g, 48 hr after birth, and were killed serially over the next 4 days. For the cell cycle following injection, the generation time was approximately 24 hr as determined by grain-count halving data; the duration of synthesis phase was 8 hr as determined from a curve constructed from the per cent of mitotic figures containing label; and the labeling index was 9% at 2 hr after injection. With these estimates, the per cent of Schwann cells proliferating was calculated to be 27%. In addition, roughly 25% of dividing cells appeared to cease division during the cell cycle under study. The relationship of these findings to other events during maturation of nerve is discussed.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002467:Cell Nucleus; D051379:Mice; D008938:Mitosis / Q000502:physiology*; D012583:Schwann Cells / Q000502:physiology*; D012584:Sciatic Nerve; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine