Amino acid concentrations in rumen fluid.
Methods using dialysis or ultrafiltration are described for the collection of extracellular fluid in rumen contents for analysis of amino acids. Marked differences in the concentration of aspartic acid, glutamic acid, and alanine were found in samples of either diffusate or ultrafiltrate and in clarified acidified rumen liquor. Concentrations are given for aspartic acid, glutamic acid, alanine, glycine, gamma-aminobutyric acid, valine, delta-aminovaleric acid, and leucine.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002247:Carbon Isotopes; D002417:Cattle; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003956:Dialysis; D004586:Electrophoresis; D012417:Rumen / Q000032:analysis*
D000596:Amino Acids; D002247:Carbon Isotopes