Chemical modification of yeast alanine-tRNA with a radioactive carbodiimide.
Yeast alanine-tRNA was reacted with 1-cyclohexyl-3-[2-morpholinyl-(4)-ethyl] carbodiimide (14)C-methoiodide in the presence of magnesium ion. The carbodiimide formed addition products with bases in the sequences psipGp, UpCp and UpUpIpGpCp. The expected bases in the sequence TppsipCpGpApUp did not react, although this region is postulated to be in a loop that is not hydrogen-bonded. The capacity of the alanine-tRNA to accept amino acids decreased after reaction with the carbodiimide.
D000409:Alanine / Q000378:metabolism*; D001345:Autoradiography; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D004586:Electrophoresis; D007094:Imides; D008274:Magnesium; D009025:Morpholines; D012343:RNA, Transfer / Q000032:analysis / Q000378:metabolism*; D013053:Spectrophotometry; D014466:Ultraviolet Rays; D015003:Yeasts
D002247:Carbon Isotopes; D007094:Imides; D009025:Morpholines; D012343:RNA, Transfer; D008274:Magnesium; D000409:Alanine