Purification and properties of staphylococcal delta-hemolysin. I. Production of delta-hemolysin.
Concentrated preparations of staphylococcal delta-hemolysin were obtained by growing selected hemolytic colonies from the 146P strain of Staphylococcus aureus on dialysis membranes laid over Brain Liver Heart agar plates at 37 C for 20 hr under 10% CO(2) and harvesting the growth from five such membranes in 1.0 ml of deionized distilled water. Incubation in a humid environment facilitated this harvesting procedure. Incubation longer than 40 hr or incubation under CO(2) higher than 10 to 20% gave lower yields of delta-lysin. Addition of a sugar, fermented by the organisms, resulted in lower yields of delta-hemolysin. Agar, although separated from the growing cells by the dialysis membrane, did potentiate delta-hemolysin production. Addition of 0.1% agar to the inoculum further enhanced this potentiation. delta-Hemolysin produced in broth or semisolid cultures was excessively diluted with the media. Dialysis membranes prevented this dilution and thus yielded concentrated preparations of delta-hemolysin.
D000362:Agar / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology; D003470:Culture Media; D003956:Dialysis; D005947:Glucose / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D009844:Oligosaccharides / Q000494:pharmacology; D013210:Staphylococcus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D006460:Hemolysin Proteins; D008567:Membranes, Artificial; D009844:Oligosaccharides; D002245:Carbon Dioxide; D000362:Agar; D005947:Glucose