Influence of isoleucine upon quinomycin biosynthesis by Streptomyces sp. 732.
When dl-isoleucine was added to an ammonium nitrate-maltose medium during cultivation of Streptomyces sp. 732, quinomycin B formation was selectively enhanced (from 3 to 70% of the quinomycin mixture) and quinomycin C synthesis was inhibited completely. In addition, two new quinomycins, designated quinomycins D and E, were produced in the presence of isoleucine. These compounds were found to contain N-methylalloisoleucine. In experiments with isoleucine enantiomorphs, it was determined that the order of effectiveness for quinomycin B synthesis was dl-isoleucine > d-isoleucine > l-isoleucine. The extent to which quinomycin B synthesis is enhanced depends upon the concentration and the time of addition of isoleucine to the medium. The effect of dl-isoleucine was reduced to some extent by the addition of l-valine. It is conceivable that amino acids which are precursors of the N-methylamino acids in quinomycin can regulate quinomycin formation.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002854:Chromatography, Paper; D007532:Isoleucine / Q000494:pharmacology*; D007930:Leucine / Q000494:pharmacology; D008715:Methionine / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*; D013912:Threonine / Q000494:pharmacology; D014364:Tryptophan / Q000494:pharmacology; D014633:Valine / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D007532:Isoleucine; D013912:Threonine; D014364:Tryptophan; D008715:Methionine; D007930:Leucine; D014633:Valine