Comparison of two methods for the separation of polar from nonpolar lipids.
The countercurrent extraction method of Galanos and Kapoulas for the separation of phospholipids from triglycerides was compared with dialysis in petroleum ether in an apparatus modified from that of Eberhagen and Betzing. The efficiencies of the methods are similar, but dialysis, which is slower, can yield triglycerides that are entirely free from phosphorus. The behavior of free fatty acids and monoglycerides is also described.
D003956:Dialysis; D005227:Fatty Acids / Q000032:analysis; D005504:Food Analysis; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D014280:Triglycerides / Q000032:analysis
D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides