Steroid metabolism in the rabbit. Biliary and urinary excretion of metabolites of [4-14-C]testosterone.
1. [4-(14)C]Testosterone was administered to anaesthetized male and female New Zealand White rabbits as a single injection or as a 45-60min. infusion. 2. After a single dose a total of approx. 56-80% of the radioactivity was excreted in bile and urine. After infusion total recovery of radioactivity was approx. 63-75%. 3. The mean ratio of metabolites in urine to those in bile was 0.77+/-0.41 (range 0.3-1.5). 4. Bile and urine samples were hydrolysed successively by beta-glucuronidase, cold acid and hot acid. In both bile and urine neutral metabolites extracted by ethyl acetate-ether were found mainly after beta-glucuronidase hydrolysis, but a considerable proportion of the dose was converted into substances not extractable from alkaline aqueous solution after all forms of hydrolysis used.
D000818:Animals; D001646:Bile; D002247:Carbon Isotopes; D002415:Cats; D005260:Female; D005966:Glucuronidase; D008297:Male; D011374:Progesterone / Q000652:urine; D011817:Rabbits; D013739:Testosterone / Q000378:metabolism* / Q000652:urine
D002247:Carbon Isotopes; D013739:Testosterone; D011374:Progesterone; D005966:Glucuronidase