A cell-free system for the study of alpha-amylase synthesis in barley aleurone layers.
1. A cell-free system capable of alpha-amylase synthesis has been obtained from the aleurone layers of germinating barley. 2. This system requires potassium chloride, sucrose and an amino acid mixture in order to function. The crude preparation does not require calcium chloride. Chloramphenicol inhibits alpha-amylase synthesis as indicated both by increase in measurable enzyme activity and incorporation of l-[U-(14)C]glutamic acid.
D000596:Amino Acids; D000681:Amylases / Q000096:biosynthesis*; D002122:Calcium Chloride; D002247:Carbon Isotopes; D002701:Chloramphenicol; D002523:Edible Grain / Q000378:metabolism*; D011189:Potassium Chloride; D013395:Sucrose
D000596:Amino Acids; D002247:Carbon Isotopes; D013395:Sucrose; D011189:Potassium Chloride; D002701:Chloramphenicol; D000681:Amylases; D002122:Calcium Chloride