Degradation of glycerophosphatides during storage of saline-washed, saline-suspended red cells at -20 degrees C.
When fresh intact red cells were washed and suspended in 0.153 M NaCl and then frozen-stored, the glycerophosphatide levels decreased significantly. Degradation began within 2 wk. Loss of phospholipid was not observed with hemoglobin-free red cell ghosts or plasma stored as long as 2 and 6 months, respectively.
D001793:Blood Preservation; D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer; D004912:Erythrocytes / Q000737:chemistry; D005615:Freezing; D006400:Hematocrit; D006452:Hemoglobinometry; D006801:Humans; D010743:Phospholipids / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D012965:Sodium Chloride
D010743:Phospholipids; D010758:Phosphorus; D012965:Sodium Chloride; D002784:Cholesterol