Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.
Antigens were prepared from Mycoplasma gallisepticum in the logarithmic phase of growth and were treated with 2, 5, and 10% Formalin by slow diffusion through a dialysis sac. Chicken erythrocytes were similarly treated with 20% Formalin. Formalin-treated antigens hemagglutinated fresh and formalinized chicken erythrocytes. The antigens retained this hemagglutinating ability over an extended period.
D000818:Animals; D000941:Antigens; D004912:Erythrocytes / Q000187:drug effects*; D005557:Formaldehyde / Q000494:pharmacology*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D009174:Mycoplasma / Q000276:immunology*; D011200:Poultry
D000941:Antigens; D005557:Formaldehyde