Method for determining antineuraminidase activity.
A test was developed to screen drugs for antineuraminidase (influenza sialidase) activity in vitro. Neuraminidase prepared from Vibrio cholerae was added to a substrate containing ganglioside, prepared from calf brain. Sialic acid is a split product in the reaction. The presence of sialic acid was detected colorimetrically by use of Warren's Thiobarbituric Acid Assay after drugs had been added to inhibit the action of neuraminidase on the calf brain substrate.
D000998:Antiviral Agents / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D002840:Chromates / Q000494:pharmacology*; D003124:Colorimetry; D004798:Enzymes / Q000494:pharmacology*; D005292:Ferricyanides; D005732:Gangliosides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D009439:Neuraminidase / Q000378:metabolism*; D011196:Potassium Permanganate / Q000494:pharmacology*; D014733:Vibrio / Q000201:enzymology
D000998:Antiviral Agents; D002712:Chlorides; D002840:Chromates; D004798:Enzymes; D005292:Ferricyanides; D005732:Gangliosides; D011196:Potassium Permanganate; D007501:Iron; D009439:Neuraminidase