A simple method for the isolation of adrenal chromaffin granules on a large scale.
Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1.6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes.
D000255:Adenosine Triphosphate / Q000032:analysis; D000313:Adrenal Medulla / Q000166:cytology*; D000818:Animals; D002395:Catecholamines / Q000032:analysis; D002417:Cattle; D002838:Chromaffin System / Q000032:analysis*; D003851:Deoxyribonucleases / Q000032:analysis; D005470:Fluorometry; D006736:Horses; D006836:Hydro-Lyases / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D051381:Rats; D013552:Swine; D014461:Ultracentrifugation
D002395:Catecholamines; D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D006836:Hydro-Lyases; D009584:Nitrogen