Separation of spores from diploid cells of yeast by stable-flow free-boundary electrophoresis.
By the use of stable-flow free-boundary (Staflo) electrophoresis and the electrophoretic mobility difference between ascospores and diploid cells of Saccharomyces cerevisiae, a mixture of the two can be separated into spore and diploid cell fractions. The spore fraction that is obtained can then be used for genetic analysis.
D004171:Diploidy; D004586:Electrophoresis; D005827:Genetics, Microbial; D011995:Recombination, Genetic; D012440:Saccharomyces / Q000032:analysis* / Q000166:cytology; D013170:Spores / Q000032:analysis*