Photoreactivation in vivo of pyrimidine dimers in paramecium DNA.
Cells of Paramecium aurelia labeled with tritiated thymidine were irradiated with ultraviolet light and then were either exposed to photoreactivating light or kept in the dark as controls. In the controls, the level of thymine-containing pyrimidine dimers did not change, but in cells exposed to photoreactivating light such dimers were destroyed. This is the first demonstration in a eukaryote of in vivo photoreactivation of thymine-containing pyrimidine dimers.
D000818:Animals; D002854:Chromatography, Paper; D004247:DNA / Q000528:radiation effects*; D010247:Paramecium / Q000528:radiation effects*; D011743:Pyrimidines / Q000032:analysis*; D011831:Radiation Genetics; D013936:Thymidine / Q000378:metabolism*; D013941:Thymine / Q000032:analysis; D014316:Tritium; D014466:Ultraviolet Rays
D011743:Pyrimidines; D014316:Tritium; D004247:DNA; D013941:Thymine; D013936:Thymidine