1,2-3-H cholesterol as a tracer in studies of human cholesterol metabolism.
1,2-(3)H-Cholesterol was fed to a subject together with 4-(14)C-cholesterol at a known (3)H/(14)C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile. Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-(3)H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.
D001646:Bile / Q000032:analysis; D001647:Bile Acids and Salts / Q000032:analysis; D002247:Carbon Isotopes; D002784:Cholesterol / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004032:Diet; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D006801:Humans; D010949:Plasma / Q000032:analysis; D014316:Tritium
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D014316:Tritium; D002784:Cholesterol