The regulation of propionate oxidation in Prototheca zopfii.
1. Whole cell suspensions of Prototheca zopfii grown on propionate oxidize propionate, acrylate, malonic semialdehyde and acetate immediately, whereas acetate-grown cells only oxidize acrylate or propionate rapidly after a lag of 20-30min. This adaptation to propionate is slowed down by 8-azaguanine or p-fluorophenylalanine, and is not influenced by adding an ammonium salt or an amino acid mixture. 2. The adaptation involves induction of the enzymes of beta-oxidation of propionate. 3. A small proportion (5-8%) of the activities of propionyl-CoA dehydrogenase, beta-hydroxypropionate dehydrogenase and malonic semialdehyde dehydrogenase are consistently associated with mitochondria isolated from propionate-grown cells. 4. Such mitochondria will oxidize propionyl-CoA, beta-hydroxypropionate and malonic semialdehyde, and the respiration rates with these substrates in the presence of inorganic phosphate are ADP-dependent. 5. Mitochondria from acetate-grown cells do not contain detectable activities of the enzymes of propionate oxidation.
D000085:Acetates / Q000378:metabolism; D000222:Adaptation, Physiological / Q000187:drug effects*; D001375:Azaguanine / Q000494:pharmacology; D004790:Enzyme Induction; D056890:Eukaryota / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*
D000085:Acetates; D011422:Propionates; D010088:Oxidoreductases; D001375:Azaguanine