Role of a cell-wall glucan-degrading enzyme in mating of Schizophyllum commune.
Mycelial enzyme extracts of Schizophyllum commune were prepared during vegetative growth matings leading to common-A and common-B heterokaryons and the dikaryon, and were examined for hydrolytic activity against an alkaliinsoluble cell-wall glucan (R-glucan) isolated from this mushroom. In extracts from several individual homokaryotic mycelia the R-glucanase activity was low and did not increase when the cultures exhausted glucose in the medium. In common-A matings, a 30-fold increase in specific activity of intracellular R-glucanase was found even in the presence of glucose in the broth. An increase of this magnitude was not observed in the common-B mating nor in the fully compatible cross leading to the dikaryon. Extracts of the dikaryon did show elevated R-glucanase activity after exogenous glucose disappearance and subsequent fruiting. In none of these situations was an enzyme activity detected towards an alkali-soluble cell-wall glucan (S-glucan) prepared from S. commune. Changes in R-glucanase were not parallelled by identical changes in laminarinase, pustulanase, cellobiase, and p-nitrophenyl-beta-d-glucosidase, but comparable increases in specific activities were found for hydrolysis of glycogen and maltose. After interaction of the various mycelia in mating combinations, the S-glucan/R-glucan ratio of the cell wall of the dikaryon was found to be similar to that of the homokaryons, but increased in the common-B interaction and was elevated almost threefold in the common-A heterokaryon.
D001487:Basidiomycota / Q000032:analysis / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D002473:Cell Wall / Q000201:enzymology; D005947:Glucose / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D005959:Glucosidases / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism; D008967:Molecular Biology; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*
D011134:Polysaccharides; D008320:Maltose; D006003:Glycogen; D005959:Glucosidases; D006026:Glycoside Hydrolases; D005947:Glucose