Homoaconitic acid accumulation by a lysine-requiring yeast mutant.
Homoaconitic acid, the second intermediate of the proposed pathway for lysine biosynthesis in yeast, is accumulated in the growth medium of a lysine-requiring mutant. This acid has been identified on paper and column chromatography by comparing it with authentic cis-homoaconitic acid. The infrared spectrum of the isolated material was identical with that of synthetic cis-homoaconitic acid. In addition, the chemical structure of the enzymatic product has been verified by degradation to glyoxylic and alpha-ketoglutaric acids after treatment with KMnO(4) and HIO(4) and by catalytic reduction to the saturated acid 1,2,4-butanetricarboxylic acid. The isolated homoaconitic acid was also identified as a substrate for a purified enzyme preparation of homoaconitase.
D000143:Acids / Q000096:biosynthesis*; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism; D008239:Lysine / Q000096:biosynthesis*; D009154:Mutation; D015003:Yeasts / Q000378:metabolism*
D000143:Acids; D002247:Carbon Isotopes; D005947:Glucose; D008239:Lysine