The calcium-stimulated incorporation of ethanolamine and serine into the phospholipids of the housefly Musca domestica.
1. The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.
D000605:Amino Alcohols / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002247:Carbon Isotopes; D006793:Houseflies / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012694:Serine / Q000378:metabolism*
D000605:Amino Alcohols; D002247:Carbon Isotopes; D008670:Metals; D010743:Phospholipids; D010759:Phosphorus Isotopes; D012694:Serine; D010740:Phospholipases; D002118:Calcium