Arginine biosynthesis by Streptococcus bovis.
The pathway of arginine biosynthesis in Streptococcus bovis was studied by radioactive tracer techniques. Cells were grown anaerobically with (14)CO(2) in a synthetic medium containing NH(4) (+) as the sole nitrogen source except for the trace present in nitrogen-containing vitamins. The protein fraction isolated from the labeled cells was acid-hydrolyzed, and (14)C-arginine was isolated from the protein hydrolysate by ion-exchange chromatography. The carboxyl carbon of the isolated arginine was removed with arginine decarboxylase, and the guanidino carbon was removed by simultaneous arginase-urease degradation. By manometric measurement and liquid scintillation counting of the CO(2) released by enzymatic degradation, 50% of the label was found in the carboxyl carbon and 50% in the guanidino carbon. Specific radioactivity determinations indicated that growth on (14)CO(2) resulted in twice as much label in arginine as with aspartate, glutamate, or lysine. These results are consistent with a glutamate --> ornithine --> citrulline pathway of arginine biosynthesis in S. bovis and provide further evidence for the synthesis of glutamate via the tricarboxylic acid cycle reactions from citrate through alpha-ketoglutarate.
D000643:Ammonium Chloride / Q000378:metabolism; D001120:Arginine / Q000032:analysis / Q000096:biosynthesis*; D001224:Aspartic Acid / Q000032:analysis; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D002952:Citric Acid Cycle; D005971:Glutamates / Q000032:analysis; D008239:Lysine / Q000032:analysis; D011492:Protein Hydrolysates / Q000032:analysis; D013291:Streptococcus / Q000378:metabolism*
D002247:Carbon Isotopes; D005971:Glutamates; D011492:Protein Hydrolysates; D000643:Ammonium Chloride; D002245:Carbon Dioxide; D001224:Aspartic Acid; D001120:Arginine; D008239:Lysine