Formation of alpha-hydroxyglutaric acid by Aspergillus glaucus.
Aspergillus glaucus, cultured on sodium propionate-mineral salts medium, incorporates (14)C-glyoxylate into labeled alpha-hydroxyglutaric acid within 30 sec. Mycelial extracts retain this biosynthetic capacity, which is destroyed by heating. Propionyl-2-(14)C-coenzyme A also in incorporated into labeled alpha-hydroxyglutaric acid by these mycelial extracts, but to a more limited extent. (14)CO(2) evolution studies, employing differentially labeled (14)C-propionate, indicate C-1 is oxidized by the mold before C-2, and C-2 before C-3. These findings suggest the involvement of alpha-hydroxyglutaric acid in the catabolism of propionic acid by A. glaucus.
D001230:Aspergillus / Q000378:metabolism*; D002245:Carbon Dioxide / Q000096:biosynthesis; D002247:Carbon Isotopes; D002474:Cell-Free System; D003065:Coenzyme A / Q000378:metabolism; D003470:Culture Media; D005650:Fumarates / Q000096:biosynthesis; D005977:Glutarates / Q000096:biosynthesis*; D006016:Glycolates / Q000096:biosynthesis; D006038:Glyoxylates / Q000378:metabolism; D006358:Hot Temperature; D008293:Malates / Q000096:biosynthesis; D011422:Propionates / Q000378:metabolism; D012492:Salts / Q000378:metabolism; D013386:Succinates / Q000096:biosynthesis
D002247:Carbon Isotopes; D003470:Culture Media; D005650:Fumarates; D005977:Glutarates; D006016:Glycolates; D006038:Glyoxylates; D008293:Malates; D011422:Propionates; D012492:Salts; D013386:Succinates; D002245:Carbon Dioxide; D003065:Coenzyme A