Intragenic mapping of chemically induced ad-7 mutants of Schizosaccharomyces pombe.
Thirty adenine-requiring ad-7 mutants of Schizosaccharomyces pombe, induced by ethylmethanesulfonate, methyl-methanesulfonate, and hydroxylamine and exhibiting low spontaneous reversion frequencies, were located by intragenic recombination analysis. Their identification as ad-7 mutants was assessed in relation to two previously mapped ad-7 mutants. Each mutant was found to occupy a distinct mutational site; the smallest recombination fraction observed between the two closest mutational sites was of the order of 0.5 x 10(-6).
D000225:Adenine / Q000378:metabolism; D001203:Ascomycota / Q000187:drug effects*; D002874:Chromosome Mapping; D006898:Hydroxylamines / Q000494:pharmacology*; D009154:Mutation; D011995:Recombination, Genetic; D013451:Sulfonic Acids / Q000494:pharmacology*
D006898:Hydroxylamines; D013451:Sulfonic Acids; D000225:Adenine