Electrophoretic properties of ovomucoid.
1. The nature of the electrophoretic heterogeneity of ovomucoid was investigated. Optimum resolution of the fractions on starch-gel electrophoresis occurred over a narrow range of pH and ionic strength. The pattern was not altered in the presence of 8m-urea but the bands were sharper. Ovomucoid-trypsin complex is stable at pH4.6 but dissociated in 6m-urea. 2. The two major fractions of ovomucoid were eluted from the gels. One of these was virtually free of sialic acid and the other, which contained 0.4mole of sialic acid/mole of protein, split into two components on electrophoresis after neuraminidase treatment. It was concluded that these two components, and likewise the two major fractions of ovomucoid, differ by a unit charge/mol. Differences in sialic acid content account for only part of the electrophoretic heterogeneity of ovomucoid.
D004529:Egg White; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D009088:Mucoproteins / Q000032:analysis*; D009438:Neuraminic Acids / Q000032:analysis; D009439:Neuraminidase; D014508:Urea
D009088:Mucoproteins; D009438:Neuraminic Acids; D014508:Urea; D009439:Neuraminidase