The separation of the alpha-chains of collagen by free-flow electrophoresis.
1. The separation of the alpha-components of codfish-skin and calf-skin collagen from the higher-molecular-weight components was accomplished by gel filtration with Bio-Gel P-300 resin in 5m-lithium chloride and at pH7.4. 2. From this fraction three distinct alpha-components, corresponding to the alpha1-, alpha2- and alpha3- chains of collagen, were isolated by free-flow electrophoresis in 6m-urea at 4 degrees . At the temperatures and pH values used there was no detectable degradation of the alpha-chains.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D002850:Chromatography, Gel; D003094:Collagen / Q000032:analysis*; D004586:Electrophoresis; D005399:Fishes; D066298:In Vitro Techniques; D008747:Methylcellulose; D010455:Peptides / Q000032:analysis*; D012867:Skin
D010455:Peptides; D008747:Methylcellulose; D003094:Collagen