Fractionation of neurophysin by molecular-sieve and ion-exchange chromatography.
Neurophysin has been separated into seven distinct protein fractions. One of these components had no hormone-binding activity. The fractions that had hormone-binding activity were similar in amino acid composition: their cystine content was in the range 11.5-14.5%. The major component, neurophysin-M, was distinguished from the protein isolated by van Dyke by the presence of methionine and the absence of histidine. Neurophysin-M binds both oxytocin and vasopressin with similar affinities.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002845:Chromatography; D002850:Chromatography, Gel; D003956:Dialysis; D004586:Electrophoresis; D006639:Histidine; D066298:In Vitro Techniques; D008715:Methionine; D010909:Pituitary Hormones, Posterior / Q000032:analysis*; D014461:Ultracentrifugation
D000596:Amino Acids; D010909:Pituitary Hormones, Posterior; D006639:Histidine; D008715:Methionine