Active cation transport in dog spermatozoa.
1. When dog semen is stored at 5 degrees for 24hr., K(+) is lost from the spermatozoa and Na(+) accumulates in the cells. 2. If at the end of the cold-storage period the semen is incubated at 37 degrees in the presence of added glucose there is a rapid uptake of K(+) and extrusion of Na(+) from the spermatozoa, the intracellular K(+) reaching a maximum within 30min. 3. When the semen is incubated at 20 degrees after cold storage there is an uptake of K(+) by the spermatozoa over 3hr. but no change in intracellular Na(+) concentration. 4. The extrusion of Na(+) and uptake of K(+) by dog spermatozoa has been shown to be inhibited by fluoride, iodoacetate, 2,4-dinitrophenol, and cetyltrimethylammonium bromide. 5. Uptake of K(+) is inhibited by ouabain and half maximum inhibition is obtained with a concentration of 50mmum. There is a slight stimulation of K(+) uptake in the presence of ouabain at about 0.3% of the concentration required for half maximum inhibition.
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D004285:Dogs; D005947:Glucose / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000378:metabolism; D012661:Semen / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D013696:Temperature
D000963:Antimetabolites; D012964:Sodium; D008274:Magnesium; D005947:Glucose; D011188:Potassium; D002118:Calcium