Effects of desensitization on the responses of WI-38 and S49 cells to hormones.
Experiments with intact cells were used to quantitate the effects of hormone desensitization on the cAMP accumulation in cultured fibroblasts (WI-38) and lymphoma cells (S49). Desensitization of WI-38 cells to prostaglandin E1 and epinephrine and of S49 cells to epinephrine was associated with a shift in the ED50 in each case to higher hormone concentrations. This led to the situation where cells under continuous stimulation by low concentrations of hormone demonstrated a greater relative decline in cAMP synthesis with time than the same cells treated with high hormone concentrations. Therefore, any general attempt to quantitate desensitization in terms of cAMP accumulation in these cells must take the hormone concentration of the assay into account.
D000527:Alprostadil; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006728:Hormones / Q000494:pharmacology*; D007700:Kinetics; D008223:Lymphoma / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology
D006728:Hormones; D011458:Prostaglandins E; D000242:Cyclic AMP; D000527:Alprostadil; D004837:Epinephrine