An improved differential pulse voltammetry technique allows the simultaneous analysis of dopaminergic and serotonergic activities in vivo with a single carbon-fibre electrode.
Differential pulse voltammetry has successfully been employed to study either 5-hydroxyindoles, or ascorbic acid and catechols in the brain of anaesthetised or freely moving rats. A new electrochemical pretreatment of pyrolytic carbon-fibre electrodes has been developed, enabling the simultaneous recording of all three compounds in the striatum of anaesthetised rats, using a Tacussel polarography. Furthermore, a fourth peak was recorded at +450 mV. Pharmacological treatments performed to define the nature of the four peaks recorded in the striatum confirmed that peak 1 corresponds to ascorbic acid, peak 2 to dihydroxyphenylacetic acid, peak 3 to 5-hydroxyindoleacetic acid and peak 4 to homovanillic acid.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D003342:Corpus Striatum / Q000032:analysis* / Q000502:physiology; D004298:Dopamine / Q000502:physiology; D004563:Electrochemistry / Q000379:methods*; D006719:Homovanillic Acid / Q000032:analysis*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000502:physiology; D009435:Synaptic Transmission
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D001205:Ascorbic Acid; D004298:Dopamine; D006719:Homovanillic Acid