title: Identification of Merkel cells in human skin by specific cytokeratin antibodies: changes of cell density and distribution in fetal and adult plantar epidermis.
abstract: Merkel cells are special neurosecretory cells which, in adult human skin, are usually very scarce. By immunofluorescence microscopy using antibodies to human cytokeratin polypeptide no. 18, we localized distinct non-keratinocyte cells in the glandular ridges of human fetal and adult plantar epidermis. Using electron and immunofluorescence microscopy, these cells were identified as Merkel cells containing typical neurosecretory granules as well as bundles of intermediate-sized filaments and desmosomes. Two-dimensional gel electrophoresis of the cytoskeletal fractions of microdissected epidermal preparations highly enriched in Merkel cells indicated the presence of cytokeratin polypeptides nos. 8, 18 and 19 which are typical of diverse simple epithelia of the human body. Double immunofluorescence microscopy showed that these human Merkel cells contain neither neurofilaments nor vimentin filaments. In human fetuses of 18-24 weeks of age, conspicuously high concentrations of Merkel cells, reaching a density of approximately 1,700 Merkel cells/mm2 skin, were found in the glandular ridges of plantar skin. The concentration decreased considerably at newborn and adult stages. Thin cell processes (up to 20 microns long) were observed in many fetal epidermal Merkel cells. In addition, we detected isolated Merkel cells deeper in the dermis (i.e. at distances of, at most, 100 microns from the epidermis) in fetal and newborn plantar skin. Our results show that Merkel cells are true epithelial cells which, however, differ profoundly from epidermal keratinocytes in their cytokeratin expression. The findings are discussed in relation to the much disputed question of the origin of Merkel cells. The present data speak against the immigration of Merkel cells from the neural crest, but rather suggest that they originate from epithelial cells of the skin, although most probably not from differentiated keratinocytes.
mesh_terms: D000375:Aging; D000911:Antibodies, Monoclonal; D000078404:Epidermal Cells; D005260:Female; D005333:Fetus / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D006651:Histocytochemistry; D006801:Humans; D007231:Infant, Newborn; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis* / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009490:Neurosecretory Systems / Q000166:cytology*; D012867:Skin / Q000166:cytology* / Q000196:embryology
chemical_list: D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D007633:Keratins