Production and initial characterization of spotted souslik (Spermophilus suslicus) interferon.
Spotted souslik (Spermophilus suslicus) interferon was induced in different cell cultures with two strains: Random (NDV-R) and Hertfordshire (NDV-H) of Newcastle disease virus. Both strains were good interferon inducers in cultures of lung cells (SL), kidney cells (SK) and embryo cells (SE). There were no significant differences in the amount of interferon produced in cells at different passage levels but the yield of interferon depended on the donor of cells. The highest interferon titers were produced 24h after infection of SK cells with NDV-R at multiplicity of infection 100 or .10 TCID50 per cell. UV-irradiation decreased the interferonogenic properties of NDV-R. Priming with 100 U/ml of 10 U/ml of homologous interferon significantly enhanced interferon production in souslik cells. However, the interferon concentrations higher than 100 U/ml caused a distinct inhibition in the subsequent interferon production ("blocking"). The pretreatment of cells with L-ascorbic acid slightly increases the yield of interferon produced in souslik cells after induction with NDV-H. Calcium chloride at nontoxic concentrations added together with the virus-inducer practically exhibited no effect on the interferon production in SL cells.
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000276:immunology; D007372:Interferons / Q000096:biosynthesis* / Q000276:immunology; D007668:Kidney / Q000276:immunology; D008168:Lung / Q000276:immunology; D009522:Newcastle disease virus / Q000276:immunology; D012589:Sciuridae / Q000276:immunology*
D007372:Interferons