The use of backscattered electrons to examine selectively stained nerve fibers in the scanning electron microscope.
Selective staining of neuronal tissues using standard light microscopic techniques has been combined with backscattered electron scanning electron microscopy. This technique allows neurons to be readily distinguished from their surrounding tissues and examined at high resolution. The technique overcomes some of the problems involved in scanning electron microscopy of nervous tissue in situ.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009474:Neurons / Q000648:ultrastructure*; D009900:Optic Nerve / Q000648:ultrastructure; D012160:Retina / Q000648:ultrastructure; D013116:Spinal Cord / Q000648:ultrastructure*; D013194:Staining and Labeling; D014981:Xenopus