A simple method for generation of antibodies with specificity for 1,25-dihydroxyergocalciferol and 1,25-dihydroxycholecalciferol.
A simple method for production of antisera with high affinity and selectivity for 1 alpha, 25-dihydroxyergocalciferol and 1 alpha, 25-dihydroxychole-calciferol is described. 1 alpha-Hydroxy-25,26,27-trisnorcholecalciferol-24-oic acid was coupled directly to bovine serum albumin. Rabbits immunized with this conjugate rapidly produced antibodies that bound 3H-1 alpha,-25-dihydroxycholecalciferol with high affinity and demonstrated nearly equal reactivity with 1 alpha, 25-dihydroxyergocalciferol and poor reactivity with 25-hydroxycholecalciferol; 24,25-dihydroxycholecalciferol; 25,26-dihydroxycholecalciferol; and 1 beta,25-dihydroxycholecalciferol. The use of one of these antisera has led to the development of a specific assay for 1 alpha,25-dihydroxyergocalciferol and 1 alpha,25-dihydroxycholecalciferol in human serum.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002117:Calcitriol / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D004872:Ergocalciferols / Q000031:analogs & derivatives* / Q000276:immunology; D007114:Immunization; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D000939:Epitopes; D004872:Ergocalciferols; D012710:Serum Albumin, Bovine; C003564:1,25-dihydroxyergocalciferol; D002117:Calcitriol