Application of antibody chimera in enzyme and erythro immunoassay.
Covalent linkage of antibodies directed against marker substances and antigen specific antibodies resulted in antibody chimera. Their usefulness was proved in an enzyme and erythro immunoassay for alpha-1-fetoprotein. Whereas the enzyme immunoassay showed the same sensitivity, precision and practicability when compared with assays using covalently linked enzyme antibody conjugates, the antibody chimera technique enables the application of crude enzyme preparations. The erythro immunoassay is well suited as screening procedure and allows also the quantitation of the antigen determining the pseudo peroxidase activity of bound erythrocytes.
D000906:Antibodies; D004912:Erythrocytes / Q000032:analysis*; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D000509:alpha-Fetoproteins; D006735:Horseradish Peroxidase