Selective degradation of heparin and heparan sulphate, and reactivity of products in the competitive binding assay.
Heparin and heparan sulphate were degraded by various chemical methods, including sodium meta-periodate oxidation, alkaline treatment, Smith degradation, and treatment with acids such as nitrous and hydrochloric acid. The products were assessed by gel filtration and in the competitive binding assay (CBA) developed recently by Dawes and Pepper (3). With the exception of nitrous acid treatment, all the chemical methods were nonselective in their effect on heparin and heparan sulphate. However, nitrous acid treatment exhibited a degree of selectivity which may be useful in enhancing the specificity of CBA. The results also highlighted the significance of sulphated groups in the binding of heparin and heparan sulphate in CBA.
D001667:Binding, Competitive; D002850:Chromatography, Gel; D006025:Glycosaminoglycans / Q000032:analysis*; D006493:Heparin / Q000032:analysis* / Q000378:metabolism; D006497:Heparan Sulfate / Q000032:analysis* / Q000378:metabolism; D006583:Hexadimethrine Bromide / Q000378:metabolism; D006851:Hydrochloric Acid; D009608:Nitrous Acid; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012972:Sodium Hydroxide
D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D006583:Hexadimethrine Bromide; D006851:Hydrochloric Acid; D009608:Nitrous Acid; D010504:Periodic Acid; D012972:Sodium Hydroxide; C009288:metaperiodate