Binding of a spin-labelled chlorpromazine analogue to calmodulin.
The binding of a spin-labelled derivative of chlorpromazine to calmodulin was investigated by e.s.r. spectrometry. The completion of the spectroscopic changes requires the presence of 4 Ca2+ ions per calmodulin molecule. The influences of various physicochemical factors (pH, ionic strength) are discussed in relation to the nature (hydrophobic and polar) of the interactions that hold the drug-calmodulin complex together.
D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin; D055598:Chemical Phenomena; D002621:Chemistry; D002746:Chlorpromazine / Q000031:analogs & derivatives*; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D011485:Protein Binding / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology
D002147:Calmodulin; D046911:Macromolecular Substances; C022892:didemethylchlorpromazine; D012965:Sodium Chloride; D002118:Calcium; D002746:Chlorpromazine