Cartilage-staining technique for the examination of unskinned fetal rat specimens previously processed with alizarin red S.
A novel staining technique has been devised that permits a cartilage examination of unskinned fetal rats that have been previously processed for skeletal examination with alizarin red S. The procedure consists of rinsing alizarin red S-stained specimens in distilled water and placing the specimens in a 3% acetic acid solution. A transfer of the stain from bone to adjacent cartilage occurs, producing purple-stained cartilaginous structures that can be differentiated from still-discernible bone structures.
D000818:Animals; D000880:Anthraquinones; D002356:Cartilage / Q000166:cytology*; D005260:Female; D005333:Fetus / Q000166:cytology*; D006652:Histological Techniques; D011247:Pregnancy; D051381:Rats; D013194:Staining and Labeling
D000880:Anthraquinones; C004468:Alizarin Red S