Echinomycin binding sites on DNA.
The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A 32P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA . Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004448:Echinomycin / Q000378:metabolism; D004586:Electrophoresis; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011810:Quinoxalines / Q000378:metabolism
D011810:Quinoxalines; D004448:Echinomycin; D004247:DNA; D004262:DNA Restriction Enzymes