A genetic map of mouse chromosome 12 composed of polymorphic DNA fragments.
Mouse chromosome 12 encodes the heavy chains of immunoglobulins (Igh), a family of T cell surface molecules, and a tumor antigen that may be homologous to immunoglobulins. To refine and extend the genetic map of this chromosome, a procedure has been developed to isolate chromosome 12-specific DNA fragments from a somatic cell hybrid carrying the chromosome on a Chinese hamster background. Five fragments have been isolated and characterized in detail. All are polymorphic, defining loci D12-1, 2, 3, 4, and 5. Using recombinant inbred mouse strains, a tentative linkage map of chromosome 12 has been worked out that incorporates these markers, the c-fos oncogene, Igh, and Pre-1/alpha 1 antitrypsin. This strategy should be applicable to any mouse chromosome or chromosomal region that can be isolated in a somatic cell hybrid.
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic
D007143:Immunoglobulin Heavy Chains; D004247:DNA; D004262:DNA Restriction Enzymes