Serial propagation of adult human prostatic epithelial cells with cholera toxin.
Reproducible subculture of adult human prostatic epithelial cells from normal, benign hyperplastic and malignant tissue has been achieved. Cholera toxin is the key component in the culture system, but use of an optimal basal medium (PFMR-4) supplemented with a high level of serum in collagen-coated dishes also improves growth and serial propagation.
D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology*; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology
D003470:Culture Media; D002772:Cholera Toxin