A new image analysis method for the quantification of neovascularization.
In a foregoing paper, an attempt has been described to quantify the neovascularization in the chorioallantoic membrane (CAM) by using the method of automatic image analysis. In this case 5 to 10 min were necessary to measure the neovascular response of one CAM preparation (i.e. to obtain a value for the degree of angiogenesis). The accuracy of reproducibility of the measurements was about +/- 20%. In this paper, we describe a new method which only requires about 55 seconds for the measurement of one specimen with an accuracy of about +/- 12%.
D000482:Allantois / Q000098:blood supply* / Q000187:drug effects; D000818:Animals; D001322:Autoanalysis; D016227:Benzoquinones; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply* / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D005321:Extraembryonic Membranes / Q000098:blood supply*; D007773:Lactates / Q000494:pharmacology; D019344:Lactic Acid; D008722:Methods; D009389:Neovascularization, Pathologic; D009993:Osmium Tetroxide / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D011809:Quinones / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000494:pharmacology
D016227:Benzoquinones; D007773:Lactates; D011809:Quinones; D011107:Polylysine; D019344:Lactic Acid; C004532:quinone; D014969:Xanthine Oxidase; D009993:Osmium Tetroxide; D003078:Colchicine