New methodology in the raising of antibodies to small peptides leading to a highly specific radioimmunoassay for enkephalins.
Temporary chemical 'protection' of the amino and/or carboxyl termini of small peptides in conjugation reactions with high molecular weight carriers has provided immunogens which give rise to antibodies with high N-terminal and/or C-terminal specificity. Applied to [Leu]enkephalin, the new methodology resulted in antisera of high affinity (K eff 0.1 x 10(12) M-1) which recognized only enkephalin-like pentapeptides. The detailed residue specificity of one enkephalin antiserum is described.
D000818:Animals; D000917:Antibody Formation; D055598:Chemical Phenomena; D002621:Chemistry; D004743:Enkephalin, Leucine / Q000032:analysis / Q000276:immunology; D004745:Enkephalins / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D004745:Enkephalins; D000939:Epitopes; D010455:Peptides; D004743:Enkephalin, Leucine