Virus-binding activity of fibronectin: masking of hepatitis A virus.
Human plasma fibronectin interacts with viruses. When fibronectin-containing human sera negative for antibodies to hepatitis A virus (HAV) were added to suspensions of HAV, radioimmunological detection of HAV was reduced. This masking effect seemed to depend on the fibronectin concentration of the sera: plasma fibronectin purified by cryoprecipitation and affinity chromatography showed a masking effect on purified HAV which was dependent on the concentrations of fibronectin and HAV. Fibronectin peptides were obtained by subtilisin digestion: the non-collagen-binding regions of the fibronectin molecule were involved in the binding of HAV. We conclude that fibronectin has a virus-binding activity which interferes with radioimmunological methods for virus detection, and may contribute to the frequent transmission of hepatitis viruses by blood products enriched in fibronectin.
D000328:Adult; D002648:Child; D005188:False Negative Reactions; D005243:Feces / Q000032:analysis; D005353:Fibronectins / Q000378:metabolism*; D006506:Hepatitis A / Q000097:blood* / Q000635:transmission; D006507:Hepatovirus / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay; D065227:Transfusion Reaction; D014766:Viremia / Q000175:diagnosis
D005353:Fibronectins