Hybridization of poly(A)+RNA from salivary glands of Rhynchosciara americana to restriction DNA fragments and polytene chromosomes.
The poly(A)+RNAs produced during DNA puff formation in the salivary gland of R. americana were used to detect the DNA sequences involved in their transcription, using the Southern hybridization and in situ hybridization techniques. DNA prepared from salivary gland after DNA puff regression and carcass were cleaved with EcoRI and hybridized to poly(A)+RNA. After hybridization two major bands corresponding to sizes of 3.0 and 6.0 kb were detected. The hybridization level in the salivary gland DNA was approximately 5-fold that observed with carcass DNA. After in situ hybridization, approximately 10 chromosome regions were labelled. The most highly labelled chromosome regions were C3d and C8e. These regions have been described as DNA puffs that undergo amplification at a specific stage of larval development.
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004175:Diptera / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes