Sequence requirements for nuclear location of simian virus 40 large-T antigen.
A point mutation in the simian virus 40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large-T antigen that was detected in the cytoplasm but not the nucleus of cells. Deletions within the surrounding sequence Lys-128Lys-Lys-Arg-Lys-Val-Glu also produce cytoplasmic large-T and define a region of the protein involved in nuclear location.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D014764:Viral Proteins; D004262:DNA Restriction Enzymes