Changes in gene expression during postnatal development of the rat cerebellum.
The base sequence complexity of total and polysomal poly(A +)RNA from rat cerebellum was measured during postnatal development by RNA-DNA hybridization. At saturation, total and polysomal poly(A +)RNA from neonate cerebellum hybridized to 12.7% and 5.0% of the single-copy genomic DNA, respectively. Assuming asymmetric transcription, the sequence complexity of these RNA populations is sufficient to code for greater than 100,000 different gene transcripts. The percentage of single-copy DNA expressed as total and polysomal poly(A +)RNA declined during postnatal development, reaching adult values of 10.0% and 4.1%, respectively. These results indicate that cerebellar maturation is accompanied by significant reductions in the diversity of genetic information expressed in the tissue.
D000818:Animals; D002531:Cerebellum / Q000254:growth & development / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA