Experimental allergic encephalomyelitis in the chicken. Class-specific antibody to myelin basic protein, the ability of T cells to cause GvH reaction, brain and spleen histology, and nonspecific biochemical indicators of inflammation.
Experimental allergic encephalomyelitis (EAE) was induced in chickens by injecting human myelin basic protein (MBP) in Freund's complete adjuvant (CFA). Antibodies of IgM, IgA and IgG class to MBP were measured by enzyme-linked immunosorbent assay (ELISA). The ability of the blood from the experimental chickens to induce graft-versus-host (GvH) reaction was tested in chick embryos. Serum concentrations of sialic acid and antitrypsin were monitored. Substantial inflammatory infiltrations were found in various parts of the central nervous system of the animals injected with MBP, but not in the controls receiving plain CFA. Enlargement of the white pulp and plasmocytosis were found in the spleens of the EAE animals. In the course of the disease, anti-MBP IgM and IgA antibody levels strongly increased, while IgG antibodies remained unchanged. The GvH caused by the blood from EAE animals did not differ from that of control birds. The EAE group showed significantly higher antitrypsin concentration than the controls. The increase in sialic acid concentration was of equal magnitude in the EAE animals and in the CFA controls.
D000818:Animals; D000917:Antibody Formation; D001921:Brain / Q000473:pathology*; D002645:Chickens; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D006087:Graft vs Host Reaction; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D004676:Myelin Basic Protein / Q000276:immunology*; D012794:Sialic Acids / Q000097:blood; D013154:Spleen / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology*; D014361:Trypsin Inhibitors / Q000097:blood
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004676:Myelin Basic Protein; D012794:Sialic Acids; D014361:Trypsin Inhibitors