[Poly(A) RNA distribution and the polysome-monosome ratio in the lymphocytes of healthy persons and chronic lympholeukemia patients].
Intact polysomes from lymphocytes of donors and patients with chronic lymphocytic leukemia (CLL) were isolated using diethyl pyrocarbonate. It has been shown that only 3/4 of ribisomes are bound to polysomes of a comparatively small size. Poly(A) RNA of the postmitochondrial fraction is largely associated with polysomes. Cyclohexamide treatment results in recruitment of monosomes into polysomes and in a shift of ribosomes in larger polysomes. There are no differences in the polysome content and size, the effect of cyclohexamide treatment and the distribution of the polysomal and non-polysomal poly(A) RNA in normal and CLL lymphocytes. It is suggested that during the transition of resting lymphocytes into the growth state, when protein synthesis increases, the mobilization of mRNP and ribosomes into polysomes is not essential.
D002458:Cell Fractionation; D002872:Chromosome Deletion; D003513:Cycloheximide / Q000494:pharmacology; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009006:Monosomy; D011061:Poly A / Q000097:blood*; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000097:blood*; D012333:RNA, Messenger
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D003513:Cycloheximide; D004047:Diethyl Pyrocarbonate
Raspredelenie poli(A) RNK i sootnoshenie polisom i monosom v limfotsitakh zdorovykh i bol'nykh khronicheskim limfoleĭkozom liudeĭ.