Detection of changes in rabbit serum proteins after partial hepatectomy by means of two-dimensional electrophoresis under non-denaturing conditions.
The changes in rabbit serum proteins after partial hepatectomy were examined by means of two-dimensional electrophoresis utilizing isoelectric focusing in a 4% polyacrylamide gel in the first dimension and a 4-30% pore gradient polyacrylamide gel in the second dimension. A rapid increase in seven proteins was observed after partial hepatectomy and a rapid decrease in two proteins. Major serum proteins, including albumin, immunoglobulin G, immunoglobulin M and alpha 2-macroglobulin, did not change. The time course of the changes was examined using a densitometer; the maxima of the changes were observed on day 3 after partial hepatectomy.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D006498:Hepatectomy; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008297:Male; D011817:Rabbits; D013194:Staining and Labeling; D013997:Time Factors
D001798:Blood Proteins