Rounding and steroidogenesis of enzyme- and ACTH-treated Y-l mouse adrenal tumor cells.
Cultured Y-l mouse adrenal tumor cells treated with ACTH (0.5 U/ml) rounded, formed filopodia and numerous thin microvilli, and produced steroids. Rounding, filopodia and bleb formation occurred for trypsin (0.01%), and hyaluronidase (0.1%), treated cells; but neither affected control or ACTH-stimulated steroidogenesis. Neuraminidase treatment (20 mU/ml) caused rounding, thin microvilli, bleb formation, slightly increased steroid production and prevented subsequent ACTH effects. Neuraminidase appeared to alter a carbohydrate-containing ACTH receptor preventing ACTH binding. We conclude rounding and steroidogenesis are not always associated.
D000310:Adrenal Gland Neoplasms / Q000503:physiopathology* / Q000648:ultrastructure; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D006867:Hydrolases / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009439:Neuraminidase / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D013256:Steroids / Q000096:biosynthesis*; D014357:Trypsin / Q000494:pharmacology
D013256:Steroids; D000324:Adrenocorticotropic Hormone; D006867:Hydrolases; D009439:Neuraminidase; D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D011402:Pronase