Application of reversed-phase HPLC in some critical peptide separations.
The application of reversed-phase high performance liquid chromatography in peptide chemistry is illustrated. Its use is described for separation of closely related endorphins, as an optical purity criterion, for rapid determination of the species of origin of insulin preparations, and finally for analysis of small, basic (hydrophilic) peptides.
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004723:Endorphins / Q000302:isolation & purification; D006801:Humans; D007328:Insulin / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D013552:Swine; D001615:beta-Endorphin
D004723:Endorphins; D007328:Insulin; D010446:Peptide Fragments; D010455:Peptides; C013765:Org 2766; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone