C-polysaccharide in a pneumococcal vaccine.
The 14-valent pneumococcal vaccine, Pneumovax, was found to contain approximately 25o micrograms per ml of the cell-wall antigen, C-polysaccharide (C-Ps), in addition to 100 micrograms per ml of each of the 14 capsular polysaccharides. Rabbit anti-C-Ps antiserum recognized four components of this C-Ps with different antigenic determinants, corresponding to four fragments with different molecular sizes. By lectin affinity chromatography it was demonstrated that some of the C-Ps in the vaccine contained cell-wall residues. The methods used in this study can also be used for the characterization of other polysaccharide preparations.
D000942:Antigens, Bacterial / Q000032:analysis; D001428:Bacterial Vaccines / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; C027158:polysaccharide C-substance (Streptococcus)