Murine hybridoma monoclonal antibodies against insulin: cross-reactivity with insulins of three species and blocking of insulin binding to its receptor.
Six hybridoma cell lines secreting monoclonal antibodies against pig insulin and cross-reacting with human and bovine insulins were obtained. Five of these monoclonal antibodies were IgG1, kappa, one IgG2b, kappa; their pI values were in the range of pH 6.3-7.4 and dissociation constants of the insulin-antibody complexes were 0.3-2 X 10(-8) mol/l, as determined by an immunoradiometric inhibition assay. All of these antibodies reacted with sterically closely related determinants and blocked the binding of 125I-pig insulin to the receptor on human MOLT-4 cell line.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D007330:Insulin Antibodies / Q000276:immunology*; D051379:Mice; D011972:Receptor, Insulin / Q000276:immunology* / Q000378:metabolism; D013045:Species Specificity; D013552:Swine
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007328:Insulin; D007330:Insulin Antibodies; D011972:Receptor, Insulin