Mapping of restriction enzyme cuts by a new two-dimensional procedure.
A new procedure has been worked out to establish restriction maps. The method is fast, does not in general require labeled DNA and has been applied to map the linear palindromic rDNA of Physarum with the restriction enzyme Bst EII.
D004247:DNA; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D010804:Physarum / Q000032:analysis*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases