Selective utilization of 2-thioribothymidine- and ribothymidine-containing tRNAs by the protein synthetic systems of Thermus thermophilus HB 8 depending on the environmental temperature.
An extreme thermophile, Thermus thermophilus HB 8, contains two types of tRNAs, T- and S2T-containing tRNAs. Their relative content changes depend on the growth temperature of the bacterial cells (1-3). To elucidate the reason why the extreme thermophile possesses the two types of tRNAs, an attempt was made to clarify how these tRNAs are utilized in in vivo protein synthetic systems of the bacteria cultured at different temperatures. First, a method was developed to isolate active polysomes from the thermophile cells cultured at 55 degrees C, 65 degrees C, and 77 degrees C. Then, tRNAs were separated from the polysomes and the T- and S2T-contents of the tRNAs were determined by HPLC. The relative content of S2T-tRNAs in the polysomes from 77 degrees C cells was much higher than that in bulk tRNAs from whole cells cultured at the same temperature, but the situation was reversed in 50 degrees C cells. These results clearly show that the protein synthetic systems of the thermophile have some selection mechanism to utilize either T- or S2T-containing tRNAs preferentially depending on the environmental temperature.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D011132:Polyribosomes / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013824:Thermus / Q000378:metabolism*; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D013891:Thiouridine; C009182:ribothymidine; C022856:2-thioribothymidine; D012343:RNA, Transfer; D014529:Uridine