Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipitated proteins: usefulness as screening test for large numbers of specimens including hybridoma supernates.
Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimens easily, (4) requires very small volumes of antigen and antibody (5-50 microliters), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000937:Antigen-Antibody Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D011233:Precipitin Tests / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D018139:Simplexvirus / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera; D014764:Viral Proteins