Biochemical and phenotypical correction of an envelope mutant of Salmonella typhimurium.
Salmonella typhimurium DA 361 bears an env D1 mutation with the following abnormal phenotypical and biochemical characteristics: a) it autolyses at stationary phase in nutrient broth; b) it grows in chains of short rods; c) it is a poor maltose fermenter and d) it has a diphosphatidylglycerol (DPG) content twice as high than its isogenic non-lytic pair DA 362 (env D+) and LT2, of which both are derivatives. Growth of DA 361 in the presence of 400 mM ethanol leads on a 50% decrease of DPG level, thereby equalling its PG/DPG ratio with those of the control strain. Consequently, a correction on the other phenotypical and biochemical anomalies are induced since the DA 361 strain decreases its autolytic activity, ferments normally maltose and appear as rods undifferentiated from DA 362.
D001329:Autolysis; D000431:Ethanol / Q000494:pharmacology; D008563:Membrane Lipids / Q000502:physiology; D009154:Mutation; D010641:Phenotype; D010743:Phospholipids / Q000502:physiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D008563:Membrane Lipids; D010743:Phospholipids; D000431:Ethanol