Processing and presentation of hen egg-white lysozyme by macrophages.
The processing and presentation by macrophages of the well-defined protein hen egg-white lysozyme (HEL) was analyzed using two HEL-specific T cell hybridomas. The processing studies revealed that both clones required that native HEL be processed, while neither clone required any processing of a tryptic digest of lysozyme. A differential requirement for processing was found for the intact, denatured lysozyme (CM-HEL) with one clone (2A11) requiring processing, and a second clone (3A9) did not require any processing. The determinant on the HEL molecule that both clones recognized was localized to a tryptic fragment containing residues 46 to 61. By testing the immunogenicity of fragments of the 46-61 peptide, mouse lysozyme, and human lysozyme, we were able to localize the T cell determinant to either of two residues, Gly-49 or Leu-56.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002645:Chickens; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009113:Muramidase / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D009113:Muramidase