Subcellular localisation of cerebral fucosyltransferase.
GDP-fucose: asialofetuin fucosyltransferase from sheep brain was fractionated on a sucrose gradient into two activity peaks. Using purification on Ficoll adapted from the proposed method [(1980) J. Neurochem. 35, 281-296], double localisation of cerebral fucosyltransferase was confirmed and the subcellular active fractions identified as light microsomes and mitochondria.
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003576:Electron Transport Complex IV / Q000032:analysis; D005647:Fucosyltransferases / Q000032:analysis*; D006602:Hexosyltransferases / Q000032:analysis*; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D012756:Sheep; D013347:Subcellular Fractions / Q000201:enzymology
D003576:Electron Transport Complex IV; D005647:Fucosyltransferases; D006602:Hexosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase; D000110:Acetylcholinesterase