Thyroid hormone regulation of poly(adenylate) polymerase activities in neuronal nuclei of developing rat brain cortex.
Neuronal and glial cell-enriched nuclei were prepared from developing rat brain cortex to study the effect of thyroxine on nuclear poly(A) polymerase. Long-term thyroxine treatment stimulated the activity of the chromatin-associated enzyme of neuronal nuclei without significantly affecting that of glial nuclei. The nuclear content of poly(A)-containing RNA in neuronal nuclei was also increased by thyroxine administrations. A single dose of thyroxine enhanced both the chromatin-bound and the free, nucleoplasmic form of neuronal poly(A) polymerase in hypothyroid rats aged 12 days. The results suggest that thyroid hormones may regulate both transcriptional and post-transcriptional events in target cell nuclei.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology / Q000254:growth & development*; D007037:Hypothyroidism / Q000201:enzymology; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011061:Poly A / Q000032:analysis; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains; D013974:Thyroxine / Q000494:pharmacology*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase; D013974:Thyroxine