A v-sis oncogene protein produced in bacteria competes for platelet-derived growth factor binding to its receptor.
The oncogene of simian sarcoma virus, v-sis, encodes a protein which is homologous to human platelet-derived growth factor (PDGF). This v-sis-encoded protein was expressed in bacteria using an inducible promotor of lambda phage. Soluble extracts from these bacteria contained a substance which competed with 125I-PDGF for PDGF receptor sites in fibroblast membranes. The receptor competition activity was correlated with the presence of the v-sis-encoded protein as assessed by genetic and immunochemical criteria. These results directly demonstrate that the v-sis oncogene product is functionally related to PDGF.
D001667:Binding, Competitive; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D020575:Oncogene Proteins v-sis; D009857:Oncogenes; D010957:Plasmids; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*
D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D014764:Viral Proteins; D017479:Receptors, Platelet-Derived Growth Factor