title: Osteoblast collagenase: collagenase synthesis by clonally derived mouse osteogenic (MC3T3-E1) cells.
abstract: Latent collagenase was isolated by heparin-Sepharose affinity chromatography from the culture medium of clonally derived mouse osteogenic (MC3T3-E1) cells. Collagenase synthesis by MC3T3-E1 cells was significantly stimulated by the addition of parathyroid hormone to the serum-containing culture medium. The cellular origin of the isolated collagenase was confirmed by demonstrating the characteristic 3/4 and 1/4 fragments of collagen alpha-chain, as well as inhibition of the enzyme by anti-mouse bone collagenase antibody.
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D007700:Kinetics; D051379:Mice; D003012:Microbial Collagenase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D010006:Osteoblasts / Q000201:enzymology*
chemical_list: D003470:Culture Media; D003012:Microbial Collagenase