Preliminary X-ray crystallographic studies of pig kidney fructose-1,6-bisphosphatase.
Preliminary x-ray data have been obtained from large single crystals of pig kidney fructose-1,6-bisphosphatase, grown from polyethylene glycol. The crystals have the symmetry of space group P3(1)21 or its enantiomorph P3(2)21, contain two subunits of the 146,000-dalton tetramer/asymmetric unit, and diffract to 2.9-A resolution on still photographs. The unit cell dimensions are a = b = 132.5 A and c = 68.0 A. Small single crystals have been grown in the presence of the inhibitor fructose 2,6-bisphosphate, with and without the allosteric effector AMP added. Crystals grown in the presence of both ligands are isomorphous with native crystals and generate diffraction patterns that show significant intensity changes.
D000818:Animals; D006597:Fructose-Bisphosphatase / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology*; D011487:Protein Conformation; D013552:Swine; D014961:X-Ray Diffraction
D006597:Fructose-Bisphosphatase