A human monoclonal antibody to cytomegalovirus (CMV).
We report the development of a human monoclonal antibody to cytomegalovirus (CMV) produced from a human X human hybridoma. This hybrid was developed by fusion of an EBV-transformed cell line making antibody to CMV and a human lymphoblastoid cell line WI-L2. The antibody is directed to a CMV-specific antigen primarily in the nucleus of CMV-infected human fibroblasts. It cross-reacts with at least 10 different strains of CMV and may provide a method for the rapid in vitro diagnosis of CMV infections. The production of CMV-specific human man monoclonal antibodies from human-human hybridomas for future therapeutic use is now technically feasible with this specific method of production.
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism; D002472:Cell Transformation, Viral; D003587:Cytomegalovirus / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D008213:Lymphocyte Activation; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral