Protective effects of diazepam and valproate on beta-vinyllactic acid-induced seizures.
GABA level and the activity of L-glutamate-1-decarboxylase (GAD) (EC 4.1.1.15) were studied in brains of mice treated with beta-vinyllactic acid, a new, selective and pyridoxal phosphate-independent GAD inhibitor. Valproate and diazepam protected mice against convulsions caused by beta-vinyllactic acid although both anti-epileptic drugs antagonized neither the decrease in GABA concentrations nor the inhibition of GAD observed after treatment with beta-vinyllactic acid alone. Assuming that the anticonvulsant effect measured with both antiepileptics is GABA mediated, these results support the hypothesis of a postsynaptic enhancement of GABAergic transmission by diazepam and valproate.
D000818:Animals; D001921:Brain / Q000187:drug effects; D003292:Convulsants / Q000037:antagonists & inhibitors* / Q000633:toxicity; D003975:Diazepam / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005968:Glutamate Decarboxylase / Q000037:antagonists & inhibitors; D007773:Lactates / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008297:Male; D051379:Mice; D012640:Seizures / Q000139:chemically induced*; D009435:Synaptic Transmission / Q000187:drug effects; D014635:Valproic Acid / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D003292:Convulsants; D007773:Lactates; D005680:gamma-Aminobutyric Acid; D014635:Valproic Acid; C042570:beta-vinyllactic acid; D005968:Glutamate Decarboxylase; D003975:Diazepam