title: Immediate-early transcription of Herpesvirus saimiri.
abstract: Transcription of Herpesvirus saimiri was characterized during the initial phases of productive infection by Northern blot analyses and hybridizations of radioactive cDNA with cloned fragments of virion L-DNA. Under conditions of immediate-early transcription, e.g., blocking of viral protein synthesis by cycloheximide, a single cytoplasmic polyadenylated viral RNA of 2.7 kilobases was found in infected cells. The sequence coding for this RNA was between map units 0.89 and 0.93; it was transcribed from right to left in prototype arrangement of M-DNA. The immediate-early mRNA of lytically infected cells appeared to be very similar, if not identical, to the single viral RNA species found in lymphoid cells transformed by H. saimiri.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics*; D008214:Lymphocytes; D009693:Nucleic Acid Hybridization; D012453:Saimiri; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes