Comparison of three conjugation procedures for the formation of tracers for use in enzyme immunoassays.
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving water-soluble carbodiimide, glutaraldehyde, and the periodate oxidation technique. Of the 3, the periodate oxidation technique proved superior giving a conjugate with only slight changes in the Michaelis constant (Km) and maximum velocity (Vmax) which could be used for an enzyme immunoassay for human placental lactogen in human plasma.
D002234:Carbodiimides; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D005260:Female; D005976:Glutaral; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010928:Placental Lactogen / Q000097:blood*; D011247:Pregnancy
D002234:Carbodiimides; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D010504:Periodic Acid; D010928:Placental Lactogen; C009288:metaperiodate; D005976:Glutaral