Chromosome deletion mapping of interspersed low-copy repetitive DNA.
A cloned 2.2 Eco RI segment of interspersed repetitive DNA was hybridized to genomic DNA from a mentally retarded patient with an interstitial deletion in the long arm of one chromosome 12 (12q-). Under hybridization conditions of high stringency, one prominent 2.2-kilobase (kb) Eco RI fragment demonstrated reduced autoradiographic intensity in the 12q- sample compared with several normal controls. These findings indicate that the genomic location for one of the highly or perfectly homologous 2.2-kb Eco RI fragments is in chromosome region 12q21q22, and suggest that a low-copy repetitive DNA probe as used here may have practical utility, as in detecting small deletions or other chromosome alterations.
D000818:Animals; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI