title: Stimulation of prostaglandin production in rat glomerular epithelial cells by antidiuretic hormone.
abstract: Prostacyclin (PGI2) and prostaglandin E2 (PGE2) production by rat glomerular epithelial cells in culture were stimulated by arginine vasopressin (AVP) over a dose range of 10(-9) to 10(-6) M, but only if the cells were allowed to recover from trypsin treatment. The effect of AVP was related to its pressor activity since the antidiuretic analogue of AVP, 1-deamino-8-D-Arg-vasopressin (dDAVP) had no effect. Angiotensin II and kallidin (lysyl-bradykinin) did not affect prostaglandin production by these cells. The stimulatory effect of AVP on arachidonate metabolism was inhibited by the calcium channel antagonist, nifedipine, in a dose-dependent fashion suggesting that cellular uptake of calcium was required.
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015232:Dinoprostone; D004847:Epithelial Cells; D011464:Epoprostenol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism; D007609:Kallidin / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D051381:Rats; D013268:Stimulation, Chemical
chemical_list: D007473:Ion Channels; D011448:Prostaglandin Antagonists; D011458:Prostaglandins E; D000804:Angiotensin II; D001127:Arginine Vasopressin; D007609:Kallidin; D011464:Epoprostenol; D009543:Nifedipine; D015232:Dinoprostone; D002118:Calcium