[Termination of DNA transcription by a poly(dA).poly(dT) fragment inserted into pBR325 plasmid].
A recombinant DNA was constructed by inserting polynucleotide (dA).(dT) of 80-100 base pairs long into EcoRI site of the pBR325 plasmid DNA. Transcription of this DNA was studied in E. coli RNA polymerase system in vitro. Some transcripts obtained with the recombinant plasmid were shown to have poly(U) clusters at the 3'-ends. Obtained data indicate that poly(dA).poly(dT) sequence acts as a terminator of RNA synthesis. Orientation of this sequence in recombinant DNA was also established.
D001482:Base Composition; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011067:Poly dA-dT / Q000235:genetics*; D011089:Polydeoxyribonucleotides / Q000235:genetics*; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D004262:DNA Restriction Enzymes
Terminatsiia transkriptsii DNK fragmentom poli(dA).poli(dT), vstroennym v plazmidu pBR325.