title: Nucleotide metabolism in spleen and peripheral blood lymphocytes during theophylline treatment.
abstract: The in vivo effect of theophylline on lymphocyte nucleotide metabolism was investigated. Rat peripheral blood lymphocytes presented more elevated levels of adenosine 5'-triphosphate (ATP) and cyclic adenosine 3',5'-monophosphate (cAMP) than shown by spleen lymphocytes. A continuous (15 days) daily oral treatment of the rats with aminophylline increased intralymphocytic cAMp content in peripheral blood (by 14 times) and in spleen lymphocytes (by 4 times), whereas ATP content was not significantly changed. NAD+ and NADH levels and NAD+/NADH ratios appeared higher in peripheral blood than in spleen cells, and were not influenced by aminophylline treatment. These results may be explained by assuming the existence of a different distribution of lymphocyte subpopulations and of a different sensitivity of the same to aminophylline treatment. The studies of nucleotide variation in peripheral blood lymphocytes incubated at 37 degrees C in anoxic conditions (N2+CO2, 95:5) revealed an impairment of ATP production and a well-preserved redox state.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism; D008156:Luciferases; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology*
chemical_list: D009711:Nucleotides; D011506:Proteins; D009243:NAD; D000255:Adenosine Triphosphate; D013806:Theophylline; D000242:Cyclic AMP; D008156:Luciferases