[Immunologic diagnosis of cutaneous lymphomas. I. A new immunoperoxidase technic for the demonstration of cell- and tissue-bound antigens in formalin-fixed paraffin-embedded tissues using protein A-peroxidase as a universal second antibody for mono- and polyclonal antibodies].
Immunoperoxidase techniques, applied to formalin-fixed paraffin embedded tissues, provide a simultaneous histopathological and immunological diagnosis. Application of protein-A-peroxidase constitutes a new technical variant: The technique is relatively simple, decreases non-specific background staining, and binds mono- and polyclonal antibodies from different mammalian species.
D001402:B-Lymphocytes; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008223:Lymphoma / Q000175:diagnosis*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis; D009195:Peroxidase; D010544:Peroxidases; D012878:Skin Neoplasms / Q000175:diagnosis*; D013205:Staphylococcal Protein A
D007527:Isoenzymes; D013205:Staphylococcal Protein A; D010544:Peroxidases; D009195:Peroxidase
Immunologische Diagnostik kutaner Lymphome. I. Eine neue Immunperoxidase-Technik zur Darstellung gewebs- und zellgebundener Antigene in formalinfixiertem Paraffin-eingebetteten Gewebe, mit Protein-A-Peroxidase als universeller "Second Antibody" für mono- und polyclonale Antiköper.