Deglycosylation by trifluoromethanesulphonic acid of endopeptidase-24.11 purified from pig kidney and intestine.
Endopeptidase-24.11, an integral microvillar membrane enzyme, exists in differently glycosylated forms when purified from pig kidney and intestine [Fulcher, Chaplin & Kenny (1983) Biochem. J. 215, 317-323]. When these glycoproteins, and another form of the kidney enzyme prepared from the Yucatan dwarf strain of piglet, were treated, under controlled conditions, with trifluoromethanesulphonic acid, the proteins were freed of carbohydrate and all had the same apparent subunit Mr (77 000) even though the untreated forms varied from Mr 89 000 to Mr 95 000.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D007422:Intestines / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D008698:Mesylates; D015260:Neprilysin; D013045:Species Specificity; D013552:Swine
D000596:Amino Acids; D002241:Carbohydrates; D008698:Mesylates; D010450:Endopeptidases; D015260:Neprilysin; C012077:trifluoromethanesulfonic acid