title: Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis.
abstract: A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C decreases CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes