Incorporation of radioactive proline and fucose in gastric mucosal biopsies. Correlation with histological findings.
Human gastric mucosal biopsies incorporate in vitro radioactive proline and fucose into macromolecular glycoproteins (mucin). Differences were found between the incorporation pattern of antral and fundic mucosae according to their pathology, confirmed by histology. Antral mucosae with abnormal histology showed a significantly higher incorporation of proline than normal samples. Fucose incorporation was also increased. Similar results were found with fundic biopsies. The ratio of total fucose to total proline incorporated into mucin secreted during incubation also increased significantly in these samples. Biochemical analyses on the other hand showed no significant change. The results suggest a breakdown in the processing, storage and secretory processes of mucin-type glycoproteins in pathological mucosae, but not in their biosynthesis. The mucosal samples could be classified as A or B types according to their proline and fucose incorporation: A mucosae have a lower proline incorporation than B mucosae (greater than 380 cpm/micrograms DNA for the antrum and greater than 200 cpm/micrograms DNA for the fundus). These results confirm the possibility of studying abnormal mucus secretion using gastric biopsy samples.
D000328:Adult; D000368:Aged; D001706:Biopsy; D004247:DNA / Q000032:analysis; D005260:Female; D005643:Fucose / Q000032:analysis / Q000378:metabolism*; D005752:Gastric Mucins / Q000096:biosynthesis; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D005767:Gastrointestinal Diseases / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011392:Proline / Q000378:metabolism*; D011506:Proteins / Q000032:analysis
D005752:Gastric Mucins; D011506:Proteins; D005643:Fucose; D004247:DNA; D011392:Proline