title: Regulation of the basolateral potassium conductance of the Necturus proximal tubule.
abstract: Two methods, the measurement of the response of the basolateral membrane potential (Vbl) of proximal tubule cells of Necturus to step changes in basolateral K+ concentration, and cellular cable analysis, were used to assess the changes in basolateral potassium conductance (GK) caused by a variety of maneuvers. The effects of some of these maneuvers on intracellular K+ activity (aiK) were also evaluated using double-barreled ion-selective electrodes. Perfusion with 0 mM K+ basolateral solution for 15 min followed by 45 min of 1 mM K+ solution resulted in a fall in basolateral potassium (apparent) transference number (tK), Vbl and aiK. Results of cable analysis showed that total basolateral resistance, Rb, rose. The electrophysiological effects of additional manipulations, known to inhibit net sodium reabsorption across the proximal tubular epithelium of Necturus, were also investigated. Ouabain caused a fall in tK accompanied by large decreases in aiK and Vbl. Lowering luminal sodium caused a fall in tK and a small reduction in Vbl. Selective reduction of peritubular sodium, a maneuver that has been shown to block sodium transport from lumen to peritubular fluid, also resulted in a significant decrease in tK. These results suggest that GK varies directly with rate of transport of the sodium pump, irrespective of the mechanism of change in pump turnover.
mesh_terms: D000818:Animals; D005260:Female; D007473:Ion Channels / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects*; D009337:Necturus / Q000502:physiology*; D009338:Necturus maculosus / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D007473:Ion Channels; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium