Nude mice injected with DNA released by antigen stimulated human T lymphocytes produce specific antibodies expressing human characteristics.
Nude mice were injected with DNA purified from the nucleoprotein complex released by T lymphocytes previously exposed in vitro to inactivated herpes or poliovirus. After five days the serum of these mice was tested for its virus neutralizing activity. Results show that injected nude mice synthesize antiherpetic or antipolio antibodies depending on the antigen used to sensitize the T lymphocytes in vitro. These antibodies were not found in the serum of uninjected control mice or mice injected with inactivated herpes or polio viruses. Mice injected with DNA released by human T cells produced antibodies carrying human allotypes since they could be neutralized by anti-allotype sera. Moreover their antiviral activity was inhibited by anti-human IgM or IgG. However, the mice which were injected with DNA released by antigen stimulated murine T lymphocytes produced antiviral antibodies which were not neutralized by anti-human allotype sera.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007126:Immunoglobulin Allotypes / Q000276:immunology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008819:Mice, Nude; D017955:Poliovirus / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007126:Immunoglobulin Allotypes; D004247:DNA