title: Substrates respired by mitochondrial fractions of two isolates of the nematode Aphelenchus avenae and the effects of electron transport inhibitors.
abstract: Electron transport has been assayed and compared in two isolates (M and F) of the free-living (model) nematode Aphelenchus avenae. Of the substrates tested only alpha-glycerophosphate and succinate were utilised to any significant extent by both isolates. Comparative data on respiratory rates, respiratory control ratios and ADP:O ratios for various substrates are given. Succinate oxidation by isolate-F mitochondria was ca 80-90% sensitive to antimycin A while that of isolate M was almost completely refractory to antimycin A. The response to other electron transport inhibitors suggests the operation of (a) azide/cyanide sensitive, (b) azide/salicylhydroxamic acid (SHAM) insensitive but carbon monoxide sensitive and (c) SHAM-sensitive terminal oxidases to varying degrees in the mitochondria of these two isolates of A. avenae.
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002248:Carbon Monoxide / Q000494:pharmacology; D002458:Cell Fractionation / Q000379:methods; D003486:Cyanides / Q000494:pharmacology; D003574:Cytochrome c Group / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D009348:Nematoda / Q000378:metabolism*; D010101:Oxygen Consumption; D012402:Rotenone / Q000494:pharmacology; D012457:Salicylamides / Q000494:pharmacology
chemical_list: D003486:Cyanides; D003574:Cytochrome c Group; D004140:Dinitrophenols; D012457:Salicylamides; D012402:Rotenone; D000968:Antimycin A; D002248:Carbon Monoxide; C005703:salicylhydroxamic acid