title: Isolation and some properties of bacteriophage alpha3 gene J mutant.
abstract: To elucidate the in vivo function of the J gene of microvirid (isometric) phages, we isolated several strains carrying a double mutation in J and H genes from phage alpha3 and then constructed single mutants each having an amber codon in the J gene. The J mutants could not multiply in suppressor-less hosts and were deficient in single-stranded progeny DNA synthesis. Nucleotide sequences of the wild-type and mutant alpha# J genes were analyzed to determine the mutation sites. The amino acid sequence of the J gene was also deduced from the nucleotide sequence and compared with those of phiX174 and G4.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D009154:Mutation; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes