A simple system for the investigation of protein binding effects on the renal handling of drugs in an isolated perfused rat kidney preparation.
An isolated perfused rat kidney preparation was developed that allowed drug-protein binding in the perfusate to be varied while maintaining renal function. When applied to digitoxin and frusemide, two drugs highly bound to bovine serum albumin, the fraction of drug unbound could be continuously varied over approximately a 30-fold range.
D000818:Animals; D003911:Dextrans / Q000378:metabolism; D004074:Digitoxin / Q000378:metabolism; D005665:Furosemide / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008722:Methods; D010477:Perfusion; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000378:metabolism
D003911:Dextrans; D004364:Pharmaceutical Preparations; D012710:Serum Albumin, Bovine; D005665:Furosemide; D004074:Digitoxin