Lack of peripheral sympathetic control of uterine blood flow during acute heat stress.
Peripheral alpha- and beta-adrenergic control of uterine blood flow (UBF) during acute heat stress of the gravid ewe was investigated. An electromagnetic blood flow probe was surgically implanted around the left miduterine artery and catheters inserted in the left carotid artery and right jugular vein in ewes between d 120 and 130 of gestation. Four or more days postsurgery, ewes were fitted with instruments to measure rectal temperature (Tr), heart rate (HR), respiratory rate (RR), blood pressure (BP) and UBF. One-half hour after instrument calibration, a 15-min thermoneutral control period was initiated with carotid artery blood samples taken at 5-min intervals for pH and PCO2 determinations. Ewes were then subjected to a heat challenge that reached 40 C at 2 h. All physiological data were recorded every 5 min as 1-min mean values. In seven experiments on five ewes, an alpha-adrenergic blocking drug, phenoxybenzamine (PB) was infused at 1 mg/min for 15 min subsequent to maximum depression of UBF. A beta-adrenergic blocking drug, propranolol (PR) was infused at .35 mg/min for 15 min in eight experiments on five ewes. Analysis of variance comparisons were made between the control period and heat stress infusion periods within the PB and PR experiments. Further comparisons were made between the start and 5, 10 or 15 min of PB or PR infusion in order to test drug effects during an acute heat stress. Rectal temperature HR, RR and arterial pH were higher (P less than .05) at the start of PR and PB infusions than during the thermoneutral control period.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001831:Body Temperature; D005260:Female; D006339:Heart Rate; D006358:Hot Temperature; D010643:Phenoxybenzamine / Q000494:pharmacology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000503:physiopathology / Q000662:veterinary*; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D012039:Regional Blood Flow; D012119:Respiration; D012756:Sheep; D012757:Sheep Diseases / Q000503:physiopathology*; D013312:Stress, Physiological / Q000503:physiopathology / Q000662:veterinary*; D013564:Sympathetic Nervous System / Q000502:physiology*; D014599:Uterus / Q000098:blood supply*
D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D010643:Phenoxybenzamine; D011433:Propranolol