Monoclonal antibody to simian virus 40 small t.
A monoclonal antibody, PAb280, was produced that recognizes simian virus 40 (SV40) small t but does not react with SV40 large T. The specificity of the antibody was analyzed by immunoprecipitation of labeled cell extracts, Western blotting, and immunocytochemistry. Small t was found to accumulate late in the SV40 lytic cycle and was localized in both the cytoplasm and the nucleus of cells infected with wild-type SV40. Importantly, antibodies against determinants common to SV40 large T and small t did not appear to be able to recognize the cytoplasmic form of SV40 small t at the immunocytochemical level. The localization of small t within the nucleus appeared to be distinct from that of large T.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000952:Antigens, Polyomavirus Transforming; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002522:Chlorocebus aethiops; D007124:Immunoenzyme Techniques; D008168:Lung; D009693:Nucleic Acid Hybridization; D013539:Simian virus 40 / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000952:Antigens, Polyomavirus Transforming; D000956:Antigens, Viral; D014764:Viral Proteins