Detection of the v-abl gene product at cell-substratum contact sites in Abelson murine leukemia virus-transformed fibroblasts.
Monoclonal antibodies to the p15 and p12 gag proteins were used to detect the P120gag-abl transforming protein of Abelson murine leukemia virus in nonproductively transformed normal rat kidney fibroblast cells. The results demonstrate that, in addition to the prominent plasma membrane location, P120gag-abl was associated with points of adhesion between the cell and the substratum. The localization of P120gag-abl was qualitatively similar to that reported for pp60src in the same normal rat kidney fibroblast cells and suggests that these transforming proteins may share some common transformation features.
D000011:Abelson murine leukemia virus / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005347:Fibroblasts / Q000502:physiology; D015683:Gene Products, gag; D005796:Genes; D005814:Genes, Viral; D007668:Kidney; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051381:Rats; D014764:Viral Proteins / Q000235:genetics*
D000956:Antigens, Viral; D015683:Gene Products, gag; D014764:Viral Proteins