Specific labeling of protein domains with antibody fragments.
Monovalent antibody Fab fragments, prepared from antisera raised against two different types of crystalline arrays made of either intact, or a proteolytic fragment of bacteriophage T4 major capsid protein, gp23*, were employed to stoichiometrically label different gp23* protein domains on the outer surface of a tubular variant (i.e., "polyheads") of bacteriophage T4 capsids. Computer filtrations of both negatively stained and freeze-dried/metal-shadowed specimens permitted approximate mapping of the Fab binding sites within the capsomere of the polyheads.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002213:Capsid / Q000276:immunology / Q000648:ultrastructure*; D000939:Epitopes / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008854:Microscopy, Electron; D011817:Rabbits; D013604:T-Phages / Q000276:immunology / Q000648:ultrastructure
D000906:Antibodies; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments