title: Interleukin 2 production induced by chemically defined cell membrane modification.
abstract: We present evidence that murine spleen cells produce a T-cell growth-stimulating factor following oxidation by periodic acid (H5IO6). The identification of this factor as interleukin 2 (IL-2) is indicated by its ability to support the growth of the IL-2-dependent CT6 cell line. In addition, preliminary analysis shows that H5IO6-stimulated growth factor has biochemical properties similar to IL-2. The time course of H5IO6-induced IL-2-like production by spleen cells was determined. No growth-stimulating activity was detected after 1 h of culture. The peak of periodic acid-induced IL-2-like production was between 18 and 24 h, while the maximum 3H-thymidine incorporation in spleen cells occurred at 72 h. Flow cytometry of CT6 cells was used for cell cycle analysis and to demonstrate their stimulation by IL-2-containing supernatants. These results were in agreement with the 3H-thymidine incorporation data. Electron microscopy of CT6 cells stimulated by supernatants from concanavalin A- or H5IO6-treated spleen cells showed no differences in their morphology. Degradation of spleen cell sialic acid prior to periodic acid oxidation inhibited IL-2-like production by 84% and inhibited 3H-thymidine uptake by 80%.
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane; D005260:Female; D005434:Flow Cytometry; D006056:Golgi Apparatus / Q000648:ultrastructure; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008854:Microscopy, Electron; D008938:Mitosis; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D012794:Sialic Acids / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
chemical_list: D007376:Interleukin-2; D012794:Sialic Acids; D010504:Periodic Acid; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid