[Stimulation of the lysogenization of Pseudomonas aeruginosa cells by phage transposon B39 induced by plasmid Rms163].
The influence of Rms163 plasmid on lysogenization of Pseudomonas aeruginosa cells by B39 phage was studied. Plasmid Rms163 was shown to increase the frequency of lysogenization of PAO1 cells 7-8 times. C-mutants of B39 phage were isolated. According to complementation test, c-mutants were distributed into two groups--cI and cII/III. The product of cI is essential for establishment and maintenance of lysogenic state, cII/cIII product being only necessary for establishment of lysogenization. The mutants with special characteristics were isolated: B39cx1 phage carries a mutation which seems to be located on a regulatory site essential for establishment of lysogenic state. The region of the B39 genome responsible for interaction with Rms163 plasmid was mapped. Possible mechanisms of Rms163 plasmid interference with transposable B39 phage are discussed.
D001435:Bacteriophages / Q000235:genetics*; D004251:DNA Transposable Elements; D005814:Genes, Viral; D005816:Genetic Complementation Test; D008242:Lysogeny; D009154:Mutation; D010957:Plasmids; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa
D004251:DNA Transposable Elements
Stimuliatsiia lizogenizatsii kletok Pseudomonas aeruginosa fagom--transpozonom B39, obuslovlennaia plazmidoĭ RMS163.