Enzymatic restriction of mammalian cell DNA using Pvu II and Bam H1: evidence for the double-strand break origin of chromosomal aberrations.
Permeabilized Chinese hamster cells were treated with the restriction enzymes Pvu II and Bam H1 which generate blunt-ended with cohesive-ended double-strand breaks in the DNA respectively. Cells were then allowed to progress to the first mitosis, where chromosomal aberrations were scored. It was found that blunt-ended double-strand breaks induced both chromosome and chromatid aberrations of exchange and deletion types, including a high frequency of tri-radials. The total aberration frequency at high enzyme concentrations was more than ten times the control background frequency. Treatment with Bam H1 on the other hand did not induce aberrations above the background rate. This may indicate that the cohesive ends generated by this enzyme may be easily repaired by the cell due to the stabilization of the hydrogen bonding at the site of the double-strand break. Measurements using the unwinding method showed that the enzymes caused strand breaks in the DNA of permeabilized cells, and an approximate X-ray dose equivalent of the restriction-enzyme-induced breaks could be calculated. This indicated that restriction-induced blunt-ended double-strand breaks are relatively inefficient in causing chromosomal aberrations. This may be because of the presence of 'clean ends' at the site of a double-strand break, which may be repaired by ligation. The method of introducing restriction enzymes into cells opens up a new model approach for the study of the conversion of double-strand breaks into chromosome aberrations.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific
D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific