Is there a "dopaminergic glial cell"?
Intracellular cAMP increased 9-fold in cerebral hemisphere primary cultures after incubation with dopamine (10(-4) M). The effect was dose- and time-dependent (10(-6) M-10(-4) M; 2-10 minutes). It was mimicked, to some extent, by the partial agonist apomorphine (10(-5) M-10(-4) M) and antagonized by fluphenazine (10(-5) M-10(-4) M). The elevation of cAMP caused by dopamine was incompletely antagonized by propanolol (10(-5) M-10(-4) M), obviating an interaction with beta-adrenergic receptors. A beta-adrenergic effect was antagonized by propranolol but only slightly by fluphenazine. The effect of dopamine on cAMP-level was more pronounced in a subpopulation of the hemisphere culture, i.e. in astroglial cultures from the striatum, 12-fold compared with controls at 10(-4) M. No dopamine stimulated formation of cAMP was found in primary cultures from brain-stem. The results demonstrated some heterogeneity among astroglial cells. The cultures used contained mainly astroglial-like cells, as judged from immunohistochemical localization of the glial specific proteins S 100 and GFA (alpha-albumin). No mature neurons or oligodendroglial cells have so far been demonstrated in the cultures.
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005476:Fluphenazine / Q000494:pharmacology; D009457:Neuroglia / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D011433:Propranolol; D000242:Cyclic AMP; D001058:Apomorphine; D005476:Fluphenazine; D004298:Dopamine; D009638:Norepinephrine