title: Lysosomal enzyme activities in hypo- and hypersecretory anterior pituitary cells. A combined immunocytochemical and enzyme cytochemical study.
abstract: The involvement of lysosomes in ACTH and prolactin secretion was studied. Lysosomes were visualized in the anterior pituitary by their non-specific esterase (gold thioacetic acid technique) or acid phosphatase (Gomori technique) activity. Corticotrophs and mammotrophs were identified by postembedding immunocytochemistry for their respective hormones. Corticotrophs were rendered hypersecretory by bilateral adrenalectomy (7 or 12 days prior to examination), hyposecretory by dexamethasone administration. Prolactin secretion was enhanced by 17-beta-estradiol, prolactin release was inhibited by bromoergocriptine administration. Long-term hypersecretion of ACTH was accompanied by the presence of numerous autophagic vacuoles often containing secretory granules in the corticotrophs. Lysosomal enzyme-containing tubules and small lysosomes were abundant in the cytoplasm near the cell membrane, among the mature secretory granules. Feed-back inhibition of ACTH release by dexamethasone resulted in the extension of enzyme-containing tubules, continuous with cisternae and small lysosomes anywhere in the cytoplasm and in the appearance of numerous crinophagic vacuoles. A higher frequency of tubular lysosomes was described at the periphery of mammotrophs stimulated by 17-beta-estradiol. Bromoergocriptine caused a high incidence of characteristic crinophagic vacuoles in the prolactin cells. The concept of crinophagy has been extended to the corticotrophs. Morphological phenomena were attributed to the traffic and increased turnover of membranes, ligands and cytoplasmic organelles during stimulated secretion.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004950:Esterases / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D006651:Histocytochemistry; D007120:Immunochemistry; D008247:Lysosomes / Q000201:enzymology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D011388:Prolactin / Q000378:metabolism; D051381:Rats
chemical_list: D001971:Bromocriptine; D004958:Estradiol; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D004950:Esterases; D000135:Acid Phosphatase