Effect of tyrosine modification on the biological and immunological properties of equine chorionic gonadotropin.
The tyrosine residues of equine chorionic gonadotropin have been nitrated with tetranitromethane and the resulting effects on the biological and immunological activities of the hormone studied. All of the tyrosine residues in equine chorionic gonadotropin were found to react with tetranitromethane when a 100-fold molar excess of reagent was used or with an 8.6 molar excess in the presence of 5 M guanidine hydrochloride. Complete nitration abolished the biological activities and decreased the immunological activity of the hormone. The nitration of one tyrosine residue resulted in the loss of 70% of the LH activity of equine chorionic gonadotropin; the FSH activity declined in a similar fashion. Maximal nitration resulted in the loss of about 50% of the immunological activity of the native hormone. Nitrated derivatives of equine chorionic gonadotropin were unable to compete with the native hormone in the rat Leydig cell assay for LH. The results indicate that the tyrosine residues of equine chorionic gonadotropin play an important role in the manifestation of both the FSH and LH activity of the hormone.
D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis; D005640:Follicle Stimulating Hormone / Q000502:physiology; D006064:Gonadotropins, Equine / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects; D007986:Luteinizing Hormone / Q000502:physiology; D008297:Male; D051381:Rats; D012671:Seminiferous Tubules / Q000187:drug effects; D013329:Structure-Activity Relationship; D013739:Testosterone / Q000096:biosynthesis; D013774:Tetranitromethane / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
D006064:Gonadotropins, Equine; D013739:Testosterone; D014443:Tyrosine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D013774:Tetranitromethane