[Growth and bioenergetics characteristics of a producer of EcoK restriction endonuclease under batch cultivation].
The conditions necessary for the controlled single- and multicycle process of the batch cultivation of E. coli CK, capable of producing E. coli CK specific endonuclease, have been established. This process can be regulated with respect to a number of parameters (temperature, pH, pO2, eH). The possibility of using thermodynamic characteristics, calculated on the basis of the redox potential and disclosing the energetics of growth, for evaluating the effectiveness of controlled batch cultivation. The optimum results have been obtained during the isolation of E. coli CK restriction endonuclease, active and containing no admixture of other endonucleases, at the period from the maximum specific growth rate to the end of the exponential growth phase, i.e. to the beginning of the stationary phase.
D000332:Aerobiosis; D002455:Cell Division; D004262:DNA Restriction Enzymes / Q000096:biosynthesis*; D015253:Deoxyribonucleases, Type I Site-Specific; D004734:Energy Metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development*; D005285:Fermentation; D013816:Thermodynamics
D004262:DNA Restriction Enzymes; C029602:endodeoxyribonuclease EcoK; D015253:Deoxyribonucleases, Type I Site-Specific
Rostovye i bioénergeticheskie kharakteristiki produtsenta restriktsionnoĭ éndonukleazy E co CK pri periodicheskom kul'tivirovanii.