Evidence for an intracellular precursor for human B-cell growth factor.
Human B-cell growth factor has been described as a trypsin-sensitive protein of Mr 12,000-14,000. Evidence is provided herein that this relatively low molecular weight product may be released from a larger precursor molecule of Mr 60,000-80,000. The precursor protein is confined to the cytosol of freshly isolated T lymphocytes, and only the Mr 12,000-14,000 moiety is released upon lectin stimulation. The precursor protein was subjected to limited tryptic digestion, which demonstrated that the biologically active fraction of the moiety resided in a relatively low molecular weight fragment. The T lymphocyte routinely possessed an intracytoplasmic pool of the precursor protein, the amount of which cyclically varied depending upon its depletion by the secretion process of a lower molecular weight product. Analysis of the mRNA size coding for the majority of B-cell growth factor activity, determined by translation in Xenopus laevis oocytes, suggested that the B-cell growth factor-specific mRNA resided in the greater than or equal to 15S range. This value is consistent with the size of the larger precursor. Therefore, it is proposed that a precursor-product relationship exists for the processing of human B-cell growth factor, analogous to that which has been described for several other cytokines.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003600:Cytosol / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D015847:Interleukin-4; D007700:Kinetics; D008222:Lymphokines / Q000096:biosynthesis / Q000235:genetics*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014357:Trypsin; D014982:Xenopus laevis
D006133:Growth Substances; D008222:Lymphokines; D010446:Peptide Fragments; D011498:Protein Precursors; D012333:RNA, Messenger; D015847:Interleukin-4; D011061:Poly A; D012313:RNA; D014357:Trypsin