Partial purification and characterization of the soluble phosphatidate phosphohydrolase of rat liver.
A method is described by which the Mg2+-stimulated phosphatidate phosphohydrolase can be purified from the soluble fraction of liver from ethanol-treated rats. The increase in specific activity was about 416-fold. This involved purification by adsorption on calcium phosphate, chromatography on DE-52 DEAE-cellulose, separation on Ultrogel AcA-34 and chromatography on CM-Sepharose 6B. The effects of phosphatidylcholine, phosphatidate and Mg2+, Mn2+ and Zn2+ on the activity are described. Inhibitor studies indicate that the phosphohydrolase contains functional thiol groups and arginine residues.
D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003512:Cyclohexanones / Q000494:pharmacology; D003931:Diacetyl / Q000494:pharmacology; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D010711:Phosphatidate Phosphatase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D010743:Phospholipids / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification*; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002413:Cations, Divalent; D003512:Cyclohexanones; D010743:Phospholipids; D013439:Sulfhydryl Reagents; C018031:1,2-cyclohexanedione; D010744:Phosphoric Monoester Hydrolases; D010711:Phosphatidate Phosphatase; D003931:Diacetyl