title: Antibodies to guanylic acid: fractionation and specificities.
abstract: Guanylic-acid-specific antibodies were elicited in rabbits, using as immunogen pG linked through 5'-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides, nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments. Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of 10(7) M-1 at 4 degrees C. Binding of the antibodies to 3H-RNA is G-specific. The antibodies do not bind to 32P-ssDNA or 32P-dsDNA. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions, though specific to pG, differ in their cross-reactivities to nucleosides and nucleotides.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D004247:DNA / Q000276:immunology; D003848:Deoxyguanine Nucleotides / Q000276:immunology; D006150:Guanine Nucleotides / Q000276:immunology*; D006157:Guanosine Monophosphate / Q000276:immunology*; D012313:RNA / Q000276:immunology; D011817:Rabbits
chemical_list: D000906:Antibodies; D003848:Deoxyguanine Nucleotides; D006150:Guanine Nucleotides; D012313:RNA; C007257:2'-deoxyguanosine 5'-phosphate; D006157:Guanosine Monophosphate; D004247:DNA