title: An embryonal carcinoma cell line as a model system to study developmentally regulated genes during myogenesis.
abstract: We have established conditions to efficiently differentiate embryonic carcinoma stem cells of the line P19 into myogenic cells. As inducers for differentiation, a combination of embryoid body formation in conjunction with treatment with dimethyl sulfoxide and retinoic acid proved to be most efficient. Under these conditions we detected an accumulation of myosin- and actin-specific RNA. Also, large amounts of type IV collagen RNA were produced. Type IV collagen is a component of the muscle basement membrane. In analogy to the F-9 system, we found a drastic decrease in stable p53 mRNA under the differentiation conditions used.
mesh_terms: D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation; D009132:Muscles / Q000196:embryology*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013724:Teratoma / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D014212:Tretinoin; D012313:RNA; D004121:Dimethyl Sulfoxide