[Use of alcian green in morphological and histochemical problems].
With hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated mucopolysaccharides (acid glycosaminoglycans) can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the mucoproteins, and after an oxydation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.
D001065:Appendix / Q000166:cytology*; D004396:Coloring Agents; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D009088:Mucoproteins / Q000032:analysis*; D010504:Periodic Acid; D011196:Potassium Permanganate; D013194:Staining and Labeling / Q000379:methods; D013447:Sulfites; D045725:Tetrapyrroles
D004396:Coloring Agents; D006025:Glycosaminoglycans; D009088:Mucoproteins; D013447:Sulfites; D045725:Tetrapyrroles; D011196:Potassium Permanganate; D010504:Periodic Acid; C044886:alcian green; C005199:potassium metabisulfite
Alciangrün-Anwendung bei morphologischen und histochemischen Problemen.