[Introduction of the hybrid plasmid RP4::D3112 into Pseudomonas putida cells requires the presence of specific mutation in the phage genome].
The wild type of D3112, a transposable phage of Pseudomonas aeruginosa can not be introduced as a portion of the hybrid plasmid RP4::D3112 into Pseudomonas putida cells. It is only possible when phage D3112 carries mutations designated lpc (lethal for P. putida and Escherichia coli). Analysis of heteroduplex molecules between DNAs of phages D3112w+ and D3112lpc demonstrated the absence of nonhomology regions, which suggests that lpc is a point mutation. The lpc2 mutation was located within the interval 20-29.9 kb of the phage genome.
D000483:Alleles; D001435:Bacteriophages / Q000235:genetics*; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006824:Hybridization, Genetic; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics
D004251:DNA Transposable Elements; D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes
Vvedenie v kletki Pseudomonas putida gibridnoĭ plazmidy RP4::D3112 trebuet nalichiia v genome faga spetsificheskoĭ mutatsii.