title: Developmental changes in the adenine nucleotide translocase in the guinea pig.
abstract: Investigations of developmental changes in energy metabolism in guinea pig liver mitochondria showed that mitochondria from the newborn were well coupled, with respiratory control ratios and membrane energy potentials similar to those obtained with mitochondria from the 1-day-old and the adult. In contrast, there was a 3-fold increase in the rate of mitochondrial respiration and a 2-fold increase in adenine nucleotide content during the first 24 h of extrauterine life. There was no significant change in the ATP/ADP ratio and only a 30% increase in the uncoupled rate of respiration during this same time period. Titrations of the adenine nucleotide translocase with the specific inhibitor, carboxyatractyloside, showed that the newborn had only 50% of the adenine nucleotide translocase activity of the adult. Furthermore, by applying flux control theory to these inhibitor titrations, it was possible to demonstrate that the adenine nucleotide translocase exerted greater control over respiration in the newborn than in the adult, and at maximal rates of coupled respiration the translocase had a control strength of 0.98. The consequences of this finding on cellular energy metabolism are discussed in relation to adaptation of the newborn to extrauterine life.
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D006168:Guinea Pigs; D007657:Ketone Bodies / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000254:growth & development*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010101:Oxygen Consumption; D013379:Substrate Specificity
chemical_list: D007657:Ketone Bodies; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases