title: Coomassie brilliant blue staining of lipids on thin-layer plates.
abstract: Coomassie brilliant blue staining of lipids on silica gel thin-layer chromatography plates is described. This stain proved to be useful for the wide-range detection of simple and complex lipids on thin-layer plates. It can stain several classes of lipids, including cholesterol, cholesterol esters, glycerides, phospholipids, ceramides, and neutral and acidic glycosphingolipids. It stains the spots evenly without a corrosive reagent, and is simple to use and suitable for storage. The visual detection limits of this stain for lipids were 0.05 to 0.5 microgram.
mesh_terms: D002855:Chromatography, Thin Layer; D003720:Densitometry; D008055:Lipids / Q000032:analysis*; D012394:Rosaniline Dyes; D013194:Staining and Labeling
chemical_list: D008055:Lipids; D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R; C004692:coomassie Brilliant Blue