Human lymphoblastoid cell lines established from peripheral blood lymphocytes secreting immunoglobulins directed against herpes simplex virus.
Peripheral blood lymphocytes (PBL) from genital herpes simplex virus type 2 (HSV-2) patients were transformed with Epstein-Barr virus. Using conditions optimized for successful transformation, two lymphoblastoid cell lines (LCL) have been established, which secrete immunoglobulin G (IgG) to HSV. These results suggest that PBL from patients suffering recurrent herpes virus infections can be used to establish LCL secreting HSV antigen specific human immunoglobulins.
D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral