Adaptation of the guaiacol (peroxidase) test for haptoglobins to a microtitration plate system.
The optimal operational method was determined for performance of an adapted guaiacol test in microtitration ELISA plates for quantitative detection of haptoglobins in sheep serum. The test correlated well with assay on Sephadex G100 but was more sensitive, though with lowered reproducibility, at low levels of haptoglobin. The modified test permits both rapid testing of large numbers of samples and the calculation of results using a simple computer program.
D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods / Q000662:veterinary*; D002850:Chromatography, Gel; D006139:Guaiacol / Q000378:metabolism; D006242:Haptoglobins / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes / Q000097:blood; D008706:Methemoglobin / Q000378:metabolism; D009195:Peroxidase; D010544:Peroxidases / Q000097:blood; D012756:Sheep / Q000097:blood*; D013696:Temperature
D006242:Haptoglobins; D007527:Isoenzymes; D006139:Guaiacol; D008706:Methemoglobin; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009195:Peroxidase