Immunogold for detection of antigen on nitrocellulose paper.
The application of immunogold for the detection of antigen-antibody complexes on nitrocellulose paper is described. Tobacco mosaic virus (TMV) protein, either purified or in leaf extract, was bound to the nitrocellulose paper and then exposed to rabbit anti-TMV serum. The antigen-antibody complexes were detected by gold-labeled goat anti-rabbit immunoglobulin G. The gold-labeled antibody is directly visible because of its pink color. This method can detect 1-5 pg of TMV protein, either in purified form or in the unpurified plant extract, with high specificity.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D003101:Collodion; D005374:Filtration; D006041:Goats; D006046:Gold; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G; D011817:Rabbits; D013194:Staining and Labeling; D014027:Tobacco Mosaic Virus / Q000276:immunology
D000936:Antigen-Antibody Complex; D000941:Antigens; D000956:Antigens, Viral; D007074:Immunoglobulin G; D006046:Gold; D003101:Collodion