title: Generation of superoxide ion in human red blood cell lysates.
abstract: The generation of superoxide ion in human red blood cell lysates was investigated by an experimental method employing Cu,Zn superoxide dismutase as O2- scavenger and EPR to probe the oxidation state of the enzyme. The average value of the O2- flux in the erythrocytes of 8 normal individuals was (2.02 +/- 0.97) X 10(-8) M S-1. A progressive saturation of the rate of O2- production was found increasing PO2, KM = 1.04 X 10(-4) M, while the autoxidation of oxyhemoglobin did not contribute significantly to the measured O2- production.
mesh_terms: D000328:Adult; D000818:Animals; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D007700:Kinetics; D010084:Oxidation-Reduction; D012016:Reference Values; D013482:Superoxide Dismutase / Q000097:blood*; D013481:Superoxides / Q000097:blood*
chemical_list: D013481:Superoxides; D013482:Superoxide Dismutase