In vitro translation of fractionated RNA isolated from chicken leukaemic plasma virus (AMV). A 76K pol-related polypeptide.
The 60S RNA was isolated from AMV-chicken leukaemic plasma virus. It was poly(A) selected, sized on sucrose gradients, and each fraction of RNA translated in vitro in the rabbit reticulocyte lysate. It was shown by immunoprecipitation and peptide mapping that p76gag was translated from genome-length RNA, a 76K pol-related polypeptide from 24-26S RNA and a 34K protein, not related to any virion proteins, from 14S RNA. The possible relationship of these in vitro translated proteins to known genome structure will be discussed.
D000956:Antigens, Viral / Q000235:genetics; D001354:Avian Leukosis Virus / Q000235:genetics*; D015683:Gene Products, gag; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics
D000956:Antigens, Viral; D015683:Gene Products, gag; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase