Propagation of rotaviruses in the presence of chicken serum.
The ability of mammalian rotaviruses to replicate in BSC-1 and CV-1 cell cultures was facilitated by the presence of 4% chicken serum. Viral plaques tended to be larger and appear more quickly than in cultures without added chicken serum. It is proposed that chicken serum facilitates plaquing of rotavirus due to its lack of trypsin inhibitors.
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000097:blood; D003470:Culture Media; D012401:Rotavirus / Q000254:growth & development*; D014361:Trypsin Inhibitors / Q000097:blood; D010948:Viral Plaque Assay; D014779:Virus Replication
D003470:Culture Media; D014361:Trypsin Inhibitors