Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins.
We have transferred the Escherichia coli gyrA and gyrB genes onto plasmids that allow the overproduction of the DNA gyrase A and B proteins and have designed relatively simple purification procedures for both proteins. The pure proteins are obtained in good yield; from 2 liters of culture (12 g of cells), one can recover 25 mg of GyrA or 3 mg of GyrB protein.
D003001:Cloning, Molecular; D004250:DNA Topoisomerases, Type II / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D010957:Plasmids
D046911:Macromolecular Substances; D004250:DNA Topoisomerases, Type II