Effect of simian virus 40 infection on albumin production by hepatocytes cultured in chemically defined medium and plated on collagen and non-collagen attachment surfaces.
Simian virus 40 (SV40)-infected rat hepatocytes were tested for albumin production (a marker of differentiated hepatocyte function) by growth in serum-free medium for 30-40 days. We observed a biphasic peak of albumin production, with the first peak at 4-6 and the second at 10-20 days after plating. A 2- to 3-fold increase in the second peak was observed when albumin production by SV40-infected cultures was compared with that by uninfected cultures. No increase in cell number in SV40-infected cultures was observed during this 20-day period. By 25-35 days, albumin production by uninfected cultures ceased but was detected in 50-60% of SV40-infected cultures. SV40-induced stimulation and persistence occurred regardless of the attachment surface and did not require collagen. Transformation occurred regardless of whether stimulation or persistence of albumin production was seen; however, stimulation and persistence were necessary to yield transformed cultures that produced albumin.
D000418:Albumins / Q000096:biosynthesis*; D000818:Animals; D002448:Cell Adhesion; D002472:Cell Transformation, Viral; D003094:Collagen; D003470:Culture Media; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013539:Simian virus 40; D013997:Time Factors; D014412:Tumor Virus Infections / Q000378:metabolism*
D000418:Albumins; D003470:Culture Media; D003094:Collagen