Effect of microinjections of cAMP-dependent protein kinase subunits on macromolecular syntheses in loach Misgurnus fossilis L. embryos.
The level of cAMP and the effects of the regulatory and catalytic subunits of cAMP-dependent protein kinase of type II on protein, RNA and DNA synthesis in loach embryos were analysed. It was found that the injections of the catalytic subunit cause a sharp decrease in the rate of macromolecular synthesis while an increase in the concentration of this protein leads to the death of the embryo. Injections of the regulatory subunit result in marked stimulation of protein, RNA and DNA synthesis. The effect of the regulatory subunit on these processes is rather complex. Experiments on the transplantation of the nuclei from the embryos treated with the protein kinase subunits into normal embryos were carried out.
D000818:Animals; D001757:Blastomeres / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005399:Fishes; D046911:Macromolecular Substances; D008845:Microinjections; D014176:Protein Biosynthesis / Q000187:drug effects*; D011494:Protein Kinases / Q000494:pharmacology*; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects*
D046911:Macromolecular Substances; D000242:Cyclic AMP; D011494:Protein Kinases