Conditions influencing the synthesis of acid protease by Mucor pusillus Lindt.
Protease synthesis by Mucor pusillus Lindt, in a wheat bran medium under submerged conditions, was influenced by substrate concentration, initial pH of the medium, and temperature of incubation. A 4% wheat bran (dry weight) concentration was satisfactory for enzyme production. The initial pH of the medium had a substantial effect on enzyme synthesis; adjustment of the enzyme production medium to pH 5.0 prior to sterilization was desirable. Incubation at 35 C resulted in the best enzyme yields. Under optimal conditions of enzyme production, maximal activity was detected after 5 days of incubation. The enrichment of the medium with glucose increased the yield of mycelia but lowered the amount of enzyme produced.
D002364:Caseins; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009089:Mucor / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D011489:Protein Denaturation; D013696:Temperature; D013997:Time Factors
D002364:Caseins; D003470:Culture Media; D010447:Peptide Hydrolases; D005947:Glucose