Development of thymic lymphomas by oral administration of N-nitroso-N-propylurea and establishment of transplantable lines of thymic lymphoma in F344 rats.
Forty-eight female F344/DuCrj rats were given a 400-ppm solution of N-nitroso-N-propylurea (CAS: 816-57-9) continuously in their drinking water. Thymic lymphomas were induced most frequently (85%) followed by duodenal tumors (48%). Sixteen tumors were examined by the immunofluorescence inhibition test for murine leukemia virus-related antigens; 15 were negative and the other was weakly positive. Twenty-six tumors were intraperitoneally and subcutaneously transplanted syngeneically; 25 (96%) were successfully transplanted intraperitoneally and 24 (92%) subcutaneously. The serial intraperitoneal transplantation was continued, and 22 lines of transplantable lymphoma in an ascites form were established. In almost all tumor lines, tumor cells took in a high percentage of recipient rats and caused the death of the recipients within 12-23 days. The thymus, liver, spleen, greater omentum, and lymph nodes were frequently invaded by transplanted tumor cells. The tumor lines were considered to be of T-cell lineage.
D000284:Administration, Oral; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D005455:Fluorescent Antibody Technique; D009052:Leukemia Virus, Murine / Q000302:isolation & purification; D008223:Lymphoma / Q000139:chemically induced* / Q000382:microbiology / Q000473:pathology; D009607:Nitrosourea Compounds / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D013953:Thymus Neoplasms / Q000139:chemically induced* / Q000382:microbiology / Q000473:pathology
D000956:Antigens, Viral; D002273:Carcinogens; D009607:Nitrosourea Compounds; C020796:propylnitrosourea