Quantitative assessment of microfilariae and adults of Onchocerca volvulus in ethanol-fixed biopsies and nodules.
Ethanol-fixed Onchocerca nodules and skin snips were successfully digested with collagenase to assess the parasite load. The importance of this technique for investigations in the field is discussed.
D000818:Animals; D006801:Humans; D003012:Microbial Collagenase; D008842:Microfilariae / Q000302:isolation & purification; D009854:Onchocerca / Q000302:isolation & purification; D009855:Onchocerciasis / Q000469:parasitology*; D012867:Skin / Q000469:parasitology
D003012:Microbial Collagenase