Human serum contains a lectin which inhibits hepatic uptake of glycoproteins.
Human serum will inhibit the specific uptake of N-acetylglucosamine-terminated glycoproteins by isolated rat hepatic sinusoidal cells. The serum inhibitors are not glycoproteins that bind to the hepatic mannose/N-acetylglucosamine receptor but have the properties of lectins which bind to mannose/N-acetylglucosamine-terminated glycoproteins. They can be isolated from serum by affinity chromatography. The predominant lectin, with a molecular mass of less than 35 kDa, will inhibit the sinusoidal cell uptake of glycoproteins in vitro.
D000818:Animals; D001212:Asialoglycoproteins; D001692:Biological Transport; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D037102:Lectins; D008099:Liver / Q000378:metabolism*; D008297:Male; D037241:Mannose-Binding Lectins; D009961:Orosomucoid / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic
D001212:Asialoglycoproteins; D006023:Glycoproteins; D037102:Lectins; D037241:Mannose-Binding Lectins; D009961:Orosomucoid; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C017030:asialoorosomucoid; C035313:mannose-N-acetylglucosamine receptor