Novel coding strategy (ambisense genomic RNA) revealed by sequence analyses of Punta Toro Phlebovirus S RNA.
Sequence analyses of Punta Toro viral S RNA species indicate the existence of a novel coding strategy for RNA viruses that involves both viral complementary and viral sense mRNA species. The Punta Toro nucleocapsid protein (N, 26.9 X 10(3) Da) is coded by a discrete viral complementary mRNA species corresponding to the 3' half of the viral S RNA. A second, presumably nonstructural, gene product (NS, 29.1 X 10(3) Da) is coded by a viral sense mRNA species that corresponds to the 5' half of the viral RNA. The ambisense nature of the S RNA is unique by comparison with any other viral RNA and raises questions concerning the family assignment of phleboviruses.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002043:Bunyaviridae / Q000235:genetics*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005814:Genes, Viral; D007668:Kidney; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000302:isolation & purification; D016856:Phlebovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000302:isolation & purification
D010446:Peptide Fragments; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA