Effect of diamide (azodicarboxylic acid-bis-dimethylamide) on arachidonic acid release from human blood platelet phospholipids.
It was found that the thiol-oxidizing agent diamide inhibits the formation of arachidonic acid metabolites in thrombin-stimulated platelets. An inhibition of arachidonic acid release from membrane phospholipids in diamide-treated platelets is concluded since diamide does not diminish the formation of stable metabolites from exogenous arachidonic acid. The role of the cellular thiol-disulfide status for the activities of phospholipases involved in arachidonic acid release is discussed.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001391:Azo Compounds / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D016861:Cyclooxygenase Inhibitors; D003958:Diamide / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005978:Glutathione / Q000097:blood; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D016859:Lipoxygenase Inhibitors; D010084:Oxidation-Reduction; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000097:blood*; D010974:Platelet Aggregation / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood
D001095:Arachidonic Acids; D001391:Azo Compounds; D016861:Cyclooxygenase Inhibitors; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D010743:Phospholipids; D003958:Diamide; D016718:Arachidonic Acid; D013929:Thromboxane B2; C025559:12-hydroxy-4,8,10-heptadecatrienoic acid; D010740:Phospholipases; D013917:Thrombin; D005978:Glutathione