Effect of estradiol, prostaglandin E2 and prostaglandin F2 alpha on incorporation of [3H]uridine by preimplantation mouse embryos in vitro.
To determine whether estrogen and prostaglandins have a direct effect on the preimplantation embryo, the effect of these compounds added to mouse embryos in vitro was examined. Embryos were incubated in culture media containing [3H]uridine with or without the substances to be tested. The addition of estradiol (10(-8)M) and prostaglandin F2 alpha (1 microgram/ml or 10 micrograms/ml) stimulated the incorporation of uridine into RNA. However, prostaglandin E2 at a concentration of 10 micrograms/ml had an inhibitory effect. These findings indicate that estradiol and prostaglandin F2 alpha may have, in addition to an indirect effect mediated by uterine factors, a direct effect on embryonic growth that activates embryos to implant.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D015237:Dinoprost; D015232:Dinoprostone; D010064:Embryo Implantation; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism
D011458:Prostaglandins E; D011460:Prostaglandins F; D004958:Estradiol; D012313:RNA; D015237:Dinoprost; D015232:Dinoprostone; D014529:Uridine