Incidence of neutrophil antigens on human cord neutrophils.
Neutrophils isolated from cord blood of healthy newborns (33 blacks and 21 whites) were investigated by EDTA-microagglutination for their expression of neutrophil specific antigens that have been associated with isoimmune neonatal or autoimmune neutropenia. Equal volumes of various neutrophil antisera (2 microliter) and cord neutrophils (3-5 X 10(6)/ml) were mixed in tissue typing microplates under oil and were incubated at room temperature for 6-8 hr, following which the degree of agglutination was noted. Our data revealed that all the currently recognized neutrophil antigens are readily demonstrable by antineutrophil antibodies in cord blood, (NA1, 52-54%; NA2, 81-85%; NB1, 95-96%; NC1, 90%; 9A, 29-30%) suggesting that neutrophil antigens are fully expressed at birth.
D000954:Antigens, Surface / Q000302:isolation & purification*; D000939:Epitopes / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D009504:Neutrophils / Q000276:immunology*
D000954:Antigens, Surface; D000939:Epitopes