Skin calcium-binding protein: effect of vitamin D deficiency and vitamin D treatment.
The amount of skin calcium-binding protein, evaluated using a sensitive radioimmunoassay and indirect immunofluorescence, was decreased in vitamin-D deficient rats and increased after one week vitamin D3 or 1 alpha-hydroxyvitamin D3 treatment. In vitamin D replete and in vitamin D-deficient animals, skin calcium-binding protein was not sensitive to changes in dietary and/or serum calcium concentrations. These results indicate that this protein is different from other calcium-binding proteins such as parvalbumin and calmodulin which are not vitamin D-dependent, and also different from intestinal calcium-binding protein which, in D replete animals, is sensitive to changes in dietary and serum calcium concentrations. Skin calcium-binding protein may, therefore, represent a new class of vitamin D-dependent protein.
D000818:Animals; D001835:Body Weight; D002118:Calcium / Q000097:blood; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002762:Cholecalciferol / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D064030:S100 Calcium Binding Protein G; D002762:Cholecalciferol; D002118:Calcium