Human chorionic gonadotropin and steroid concentrations in human follicular fluid in relation to follicle size and oocyte maturity in stimulated ovarian cycles.
The concentrations in follicular fluid (FF) of beta hCG, progesterone (P), estradiol (E2), androstenedione (A) and testosterone (T) by RIA were determined, in order to study the follicular micro-environment in stimulated cycles. Patients received either clomiphene with hMG plus hCG or hMG plus hCG. With increasing volume of FF (obtained at laparoscopy), P significantly increased (p less than 0.04) and hCG (p less than 0.0004) and T (p less than 0.05) significantly decreased with a borderline decrease in A (p less than 0.07). Progesterone also increased with advancing oocyte maturity. Beta-hCG and P were positively correlated with E2 and negatively correlated with A levels. It is suggested that FF hCG and progesterone levels may be markers of follicular development in stimulated cycles.
D000735:Androstenedione / Q000032:analysis*; D001826:Body Fluids / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000627:therapeutic use; D018997:Chorionic Gonadotropin, beta Subunit, Human; D002996:Clomiphene / Q000627:therapeutic use; D004958:Estradiol / Q000032:analysis*; D005260:Female; D005498:Follicular Phase; D006801:Humans; D008596:Menotropins / Q000627:therapeutic use; D009865:Oocytes / Q000254:growth & development*; D006080:Ovarian Follicle / Q000032:analysis*; D010062:Ovulation Induction; D010446:Peptide Fragments / Q000032:analysis*; D011374:Progesterone / Q000032:analysis*; D011863:Radioimmunoassay; D013739:Testosterone / Q000032:analysis*
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; D002996:Clomiphene; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D008596:Menotropins