Calcium efflux parallel to total phosphate retention in rat liver mitochondria.
Phosphate efflux from uncoupled rat liver mitochondria was completely inhibited when mersalyl plus butylmalonate and ATP were added to a sucrose suspending medium. Despite the total retention of phosphate a calcium efflux was observed even in presence of ruthenium red. Under the above conditions no phosphate is transported in association with the ADP/ATP carrier. While mersalyl completely blocked the phosphate release induced by ruthenium red or EGTA from coupled mitochondria it only partially inhibited the CA2+-efflux. The inhibition of Ca2+ efflux was almost completely abolished in the presence of acetate. The existence of a co-transport of Ca2+ associated with phosphate is discussed.
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D008314:Malonates / Q000494:pharmacology; D008634:Mersalyl / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology
D008314:Malonates; D010710:Phosphates; D012430:Ruthenium Red; C027734:n-butylmalonic acid; D008634:Mersalyl; D002118:Calcium