Kinetics of the interaction of monophosphates of the antiviral nucleosides 2'-fluoro-1-beta-D-arabinofuranosylpyrimidine and (E)-5-(2-bromovinyl)-2'-deoxyuridine with thymidylate kinases from Vero cells and herpes simplex virus types 1 and 2.
The affinities of the monophosphates of 2'-fluoro-5-iodo-1-beta-D-arabinofuranosyluracil and its 5-methyl analog for cellular thymidylate kinase were two or more orders of magnitude greater than for the thymidine-thymidylate kinases from herpes simplex virus types 1 and 2. In contrast, the monophosphate of (E)-5-(2-bromovinyl)-2'-deoxyuridine was found to have a higher affinity for the viral enzymes than for the cellular enzyme.
D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D001088:Arabinonucleotides / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D004347:Drug Interactions; D006801:Humans; D007700:Kinetics; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000378:metabolism*; D001084:Vidarabine Phosphate / Q000378:metabolism*
D000998:Antiviral Agents; D001088:Arabinonucleotides; D001084:Vidarabine Phosphate; C038492:5-(2-bromovinyl)-3-amino-2',3'-dideoxyuridine; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine