Quantitative autoradiography of [3H]sulpiride binding sites in rat brain.
A technique has been developed to investigate [3H]sulpiride binding in rat brain sections using quantitative autoradiography and tritium-sensitive film. Binding was saturable and reversible with very low nonspecific binding. [3H]Sulpiride bound to an apparent single population of sites in striatum with a Kd of 3.2 nM and Bmax of 447 fmol/mg protein. Binding sites were localized in the lamina glomerulosa of the olfactory bulb, nucleus accumbens, olfactory tubercle, striatum and substantia nigra.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002069:Butaclamol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013469:Sulpiride / Q000378:metabolism*; D014018:Tissue Distribution; D014316:Tritium
D014316:Tritium; D013469:Sulpiride; D002069:Butaclamol