Enzymatic synthesis of flavin adenine dinucleotide.
A new and simple enzymatic method for the synthesis of flavin adenine dinucleotide (FAD) from flavin mononucleotide by the transadenylylation reaction using microbial cells is described. Among various microorganisms tested, Artherobacter globiformis IFO 12138 and two soil bacteria were selected as useful enzyme sources. Under suitable reaction conditions, the amount of FAD synthesized was 2.25 mumol/mL with cells of A. globiformis. The transadenylylation reaction could be coupled with the ATP supplying system through a glycolysis process with yeast.
D001173:Arthrobacter / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology; D005182:Flavin-Adenine Dinucleotide / Q000096:biosynthesis*; D007202:Indicators and Reagents; D009713:Nucleotidyltransferases / Q000378:metabolism*; D012988:Soil Microbiology; D013045:Species Specificity
D007202:Indicators and Reagents; D005182:Flavin-Adenine Dinucleotide; D009713:Nucleotidyltransferases; C020143:FMN adenylyltransferase