Degradation of bovine P2 protein by bovine brain cathepsin D.
Bovine brain cathepsin D cleaved bovine P2 protein to produce three major and several minor peptides. The major P2 peptides formed were shown by amino acid analysis and partial sequencing to be peptides 17-54, 20-58 and 65-131 with the latter predominating. In preliminary experiments, P2 peptide 65-131 did not induce experimental allergic neuritis in Lewis rats in equimolar amounts to the neuritogenic P2.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002384:Catalysis; D002402:Cathepsin D; D002417:Cattle; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology / Q000302:isolation & purification; D018996:Myelin P2 Protein; D051381:Rats; D011917:Rats, Inbred Lew
D000596:Amino Acids; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein; D002402:Cathepsin D