[Expressions of two types of soluble cAMP-dependent protein kinases of the rabbit myometrium in different functional states].
Two types of soluble cAMP-dependent protein kinase (I and II) were isolated from rabbit myometrium cytosol at functional rest and characterized. In pregnancy, protein kinase is represented by type II alone. Upon delivery, one isoform of the enzyme was detected, which was eluted from a DEAE-cellulose column with 0.15-0.22 M. NaCl. During the postnatal period, the elution profile of the enzyme is made up of two protein bands, one fraction being eluted with 0.15-0.22 M NaCl (93% of total enzyme content), and the other one being represented by a minor component eluted with 0.07-0.09 M. NaCl (7%). In terms off isoenzyme activity, main kinetic properties, ability to autophosphorylate and Kass for cAMP, the protein kinase isolated during delivery and the major protein kinase fraction obtained in the postnatal period can be related to protein kinases type II. Quantitative and qualitative expression of two types of soluble cAMP-dependent protein kinase from rabbit myometrium isolated at different functional states may be due to differences in their biological activity.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D000242:Cyclic AMP / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D006657:Histones / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D009215:Myometrium / Q000201:enzymology*; D010766:Phosphorylation; D011247:Pregnancy; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits
D006657:Histones; D007527:Isoenzymes; D000242:Cyclic AMP; D011494:Protein Kinases
Ekspressiia dvukh tipov rastvorimoĭ cAMP-zavisimoĭ proteinkinazy miometriia krol'chikh pri razlichnykh funktsional'nykh sostoianiiakh.