title: Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus.
abstract: Herpes simplex virus isolation by conventional cell culture in primary rabbit kidney cells (RK) was compared with Cultureset (CS). At 24 h CS was more sensitive (31 versus 25 of 55 isolates detected) than RK, but at 48 h there was little difference (43 detected by CS, 46 by RK). CS stained at 48 h was significantly less sensitive (78.2%) than RK observed for 1 week. CS stained at 24 or 48 h was not an acceptable substitute for RK observed for 1 week.
mesh_terms: D000818:Animals; D003365:Costs and Cost Analysis; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis*; D006561:Herpes Simplex / Q000175:diagnosis*; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D011933:Reagent Kits, Diagnostic; D018139:Simplexvirus / Q000302:isolation & purification / Q000502:physiology
chemical_list: D011933:Reagent Kits, Diagnostic