Antibiotic production of hyphal fractions of Streptomyces griseus. II. Streptomycin production of different fractions obtained by density gradient centrifugation.
The streptomycin-producing activity (SPA) of hyphal fractions from washed mycelium of submerged cultures of Streptomyces griseus strain 52-1, as obtained by density gradient centrifugation, was investigated. Activity of the various fractions differed strongly in intensity. The highest SPA was evident in the unfractionated mycelium. A synergistic effect upon SPA was found in the interaction of cultures of different ages, and a 55% increase in yield was obtained by mixing the 48- and 72-hr cultures. A synergistic effect occurred in all combinations studied. By use of fractions obtained from 72-hr mycelium for inoculation, differences in streptomycin production were noted. Some inoculum fractions yielded a greater amount of streptomycin (36%) than the unfractionated mycelial inoculum.
D002499:Centrifugation, Density Gradient; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D013307:Streptomycin / Q000096:biosynthesis*
D013307:Streptomycin