Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.
Chimeric SV40 DNA containing only the early region, or plasmid DNA harboring the origin-promoter-enhancer region of SV40, when introduced into CV-1 or Cos-1 monkey cells by DEAE-dextran mediated transfer are rapidly assembled in a typical chromatin structure revealed by the generation of a regular 190 bp repeat ladder after micrococcal nuclease digestion. DNA replication is not required for this assembly process. Chromatin-specific DNase I hypersensitive sites are observed in the enhancer region of these minichromosomes. The pattern of the sites differs between non-replicating and post-replicated chromatin. The latter is identical to that observed in the lytic cycle. The presence of large T antigen is not sufficient for the shift in the structure of the chromatin. These experiments suggest that replication can modulate protein-DNA interactions during viral infection or upon cell differentiation.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D002843:Chromatin / Q000502:physiology*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis; D004279:DNA, Viral / Q000235:genetics*; D007668:Kidney; D009876:Operon; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D002843:Chromatin; D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes