title: Quantification of circular DNA breakage by fluorescence scanning of ethidium bromide-stained horizontal gels: application to the effect of intercalating agents.
abstract: Direct scanning of the fluorescence of DNA in ethidium bromide-stained agarose gels allowed the quantification of closed and open circular DNA forms. Fluorescence of form I was higher than expected compared to form II. Application of this technique is shown for an intercalating drug treatment of DNA.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D004270:DNA, Circular / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004996:Ethidium; D007364:Intercalating Agents / Q000494:pharmacology*; D008433:Mathematics; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D004270:DNA, Circular; D007364:Intercalating Agents; D004996:Ethidium