Quantitative and ultrastructural studies on the uptake of drug loaded liposomes by mononuclear phagocytes infected with Leishmania donovani.
This study compared splenic and hepatic uptake of free and liposome-entrapped sodium antimony gluconate after i.v. administration to mice infected with Leishmania donovani. It was demonstrated that entrapment within liposomes greatly altered the kinetics of uptake of the drug. We were also able to show that liposomes composed of sphingomyelin, stearylamine and cholesterol were marginally better than any other preparation in delivering entrapped drug to liver and spleen. X-ray microanalytical studies on the uptake of liposomes by Kupffer cells infected with L. donovani have indicated that internalised liposomes probably fuse with parasitophorous vacuoles, transferring their contents into the immediate locality of the leishmanial parasites. It is proposed that this is the way in which liposome entrapped antileishmanial agents have an enhanced therapeutic effect over free drug therapy.
D000818:Animals; D000967:Antimony Sodium Gluconate / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism*; D007898:Leishmaniasis, Visceral / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D010586:Phagocytes / Q000378:metabolism* / Q000469:parasitology / Q000648:ultrastructure; D013054:Spectrophotometry, Atomic; D013154:Spleen / Q000378:metabolism; D014617:Vacuoles / Q000469:parasitology
D005942:Gluconates; D008081:Liposomes; D000967:Antimony Sodium Gluconate