Simian sarcoma virus transformation-specific glycopeptide: immunological relationship to human platelet-derived growth factor.
The simian sarcoma virus transformation-specific glycopeptide (SSV-TrSgp) represents a proteoglycan which is released from SSV-transformed cells and can be detected by an autologous goat serum against SSV nonproducer cells (SSV-NP serum) (H.-J. Thiel, R. Hafenrichter, and B. Gregor, 1984, Virology 134, 138-147). This antiserum has now been shown to react also with human platelet-derived growth factor (PDGF). Antiserum to PDGF precipitated a glycosylated molecule from the tissue culture supernatant of SSV-NP cells. The respective antigen was identified as the SSV-TrSgp (after immunoprecipitation including enzymatic treatment with chondroitinases). The anti-SSV-TrSgp reactivity of both the anti-PDGF serum and the SSV-NP serum could be absorbed by pure PDGF. Therefore, the SSV-TrSgp is apparently immunologically related to human PDGF. Additional studies indicated that the SSV-TrSgp protein backbone and PDGF have very similar molecular weights.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D006026:Glycoside Hydrolases; D006801:Humans; D007668:Kidney; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D051381:Rats; D012190:Retroviridae / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics*; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000032:analysis*
D010982:Platelet-Derived Growth Factor; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; C042358:simian sarcoma virus transformation-specific glycopeptide; D006026:Glycoside Hydrolases