The histone H5 gene is flanked by S1 hypersensitive structures.
The potential of the cloned histone H5 gene to form altered DNA structures has been examined by S1 nuclease digestion of supercoiled recombinant plasmids containing up to 8.8 kbp of chicken DNa. The three main nicking sites map at the upstream and downstream sequences flanking the structural gene. The cleavage sites share sequence homology, strand specificity, and do not seem to be single-stranded. The sequence of the S1-sensitive sites does not suggest that the fragments can adopt any of the known DNA secondary structures.
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004278:DNA, Superhelical / Q000235:genetics; D004720:Endonucleases / Q000378:metabolism*; D005796:Genes; D006657:Histones / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004274:DNA, Recombinant; D004278:DNA, Superhelical; D006657:Histones; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases