Analysis of a serological determinant of H-Y antigen: evidence for carbohydrate specificity using an H-Y specific monoclonal antibody.
Using a monoclonal antibody to H-Y antigen we characterized the molecular determinant responsible for H-Y reactivity. H-Y-positive cells were treated with enzymes which alter carbohydrate structure and then were tested for their ability to absorb monoclonal anti-H-Y antibody. Indications are that the serological determinant recognized by this antibody is a glycoconjugate containing terminal non-reducing and internal galactosyl residues.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002241:Carbohydrates / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D005260:Female; D005690:Galactose / Q000276:immunology; D006182:H-Y Antigen / Q000276:immunology*; D008297:Male; D051379:Mice; D051381:Rats; D012867:Skin / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D000939:Epitopes; D006182:H-Y Antigen; D005690:Galactose