title: Monoclonal antibodies that distinguish between human cellular and plasma fibronectin.
abstract: Two monoclonal antibodies have been developed against human fibronectin expressed by simian virus 40 transformed human mammary epithelial cells. Monoclonal antibody ICRF-FN-3 recognizes a determinant on human cellular but not plasma fibronectin. The antigenic site recognized by FN-3 is lost after limited proteolysis of cellular fibronectin with alpha-chymotrypsin but not trypsin. Monoclonal antibody, ICRF-FN-4, against a determinant on both human plasma and cellular fibronectin reacts with an antigenic site on fibronectin which is not destroyed by trypsin or alpha-chymotrypsin. Although the epitopes recognized by FN-3 and FN-4 are expressed similarly by cells from the human breast they are expressed differently on rat mammary (RAMA) cell lines.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001940:Breast / Q000032:analysis; D002478:Cells, Cultured; D002918:Chymotrypsin; D000939:Epitopes; D005260:Female; D005353:Fibronectins / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D051381:Rats; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005353:Fibronectins; D002918:Chymotrypsin