Hydroxyl radical production and DNA damage induced by anthracycline-iron complex.
Adriamycin-Fe3+ complex catalyzes the formation of hydroxyl radical from hydrogen peroxide but the DNA-adriamycin-iron ternary complex is much more effective. 11-Deoxyadriamycin, which shows no spectral evidence of complex formation with iron, was ineffective. The generation of hydroxyl radical by adriamycin-Fe3+ complex in the presence of DNA correlates with its ability to cleave DNA. Hydroxyl radicals are thus implicated as the reactive oxygen species involved in the DNA damage caused by the adriamycin-Fe3+ complex.
D003497:Cyclic N-Oxides; D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D013053:Spectrophotometry; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005290:Ferric Compounds; D006878:Hydroxides; D013113:Spin Labels; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; C042239:11-deoxyadriamycin; D004317:Doxorubicin; D004247:DNA; D006861:Hydrogen Peroxide; D007501:Iron