[Fluorescence of hemoproteins in reversed micelles of surfactants in octanes].
The fluorescence of myoglobin, cytochromes b5 and c in the reversed aerosol OT (AOT) micelles in octane has been investigated. The fluorescence intensity of all the three hemoproteins is higher than that in aqueous solutions. The maxima and intensities of fluorescence in the AOT micelles depend on the [H2O]/[AOT] ratio and reflect the protein structure. Aliphatic alcohols and secondary amines (piperidine and morpholine) quench the cytochrome c fluorescence in the AOT micelles, whereas dipolar aprotic solvents (dimethylsulfoxide, dimethylformamide) significantly increase the intensity of cytochrome c fluorescence in the same micelles. The transformations of the proteins solubilized by the reversed micelles of a surfactant are discussed.
D000818:Animals; D003102:Colloids; D003573:Cytochrome b Group / Q000032:analysis*; D003574:Cytochrome c Group / Q000032:analysis*; D015786:Cytochromes b5; D004143:Dioctyl Sulfosuccinic Acid / Q000494:pharmacology; D005453:Fluorescence; D006736:Horses; D008823:Micelles; D009025:Morpholines / Q000494:pharmacology; D009211:Myoglobin / Q000032:analysis*; D009795:Octanes; D010880:Piperidines / Q000494:pharmacology; D011817:Rabbits; D012996:Solutions; D013501:Surface-Active Agents / Q000494:pharmacology*; D014907:Whales
D003102:Colloids; D003573:Cytochrome b Group; D003574:Cytochrome c Group; D008823:Micelles; D009025:Morpholines; D009211:Myoglobin; D009795:Octanes; D010880:Piperidines; D012996:Solutions; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; C032727:piperidine; C037574:morpholine; D015786:Cytochromes b5
Fluorestsentsiia gemoproteidov v obrashchennykh mitsellakh poverkhnostno-aktivnykh veshchestv v oktane.