Effects of detergents on indirect assays for superoxide dismutase activity.
The effects of five detergents on the ferricytochrome c reduction and on the nitroblue tetrazolium reduction to monoformazan as well as on the o-dianisidine photooxidation was examined. Triton X-100 and Lubrol augmented the formazan formation. Sodium dodecylsulphate suppressed it, whereas digitonin and sodium deoxycholate were almost ineffective no matter whether superoxide anion radicals were photochemically, chemically or enzymatically generated. The five detergents were approximately equally effective on both aerobic and anaerobic nitroblue tetrazolium photoreduction. They inhibited o-dianisidine photooxidation and did not effect the ferrycytochrome c reduction. It is assumed that the detergents used interfered with indirect superoxide dismutase assay systems.
D000332:Aerobiosis; D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology*; D003962:Dianisidine / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003574:Cytochrome c Group; D003902:Detergents; D013501:Surface-Active Agents; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D003962:Dianisidine