Studies on the properties of glucose-6-phosphatase from carp liver microsomes (Cyprinus carpio L.).
The effects of temperature and pH on the phosphohydrolase activity of carp hepatic glucose-6-phosphatase (EC 3.1.3.9) have been investigated. The enzyme activity was maximum at about 308 K and in the pH range 5-6.5. The apparent Michaelis constant (KM) and Vmax of the reaction with glucose-6-phosphate were found to be 14.8 mM and 2.27 nmol/min/mg protein. The enzyme activity was partly inhibited by EDTA, while in the presence of sufficient PCMB virtually total inhibition was observed.
D000818:Animals; D002347:Carps; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D013816:Thermodynamics; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D020245:p-Chloromercuribenzoic Acid; D004492:Edetic Acid; D005952:Glucose-6-Phosphatase