Identification of a bovine rotavirus gene and gene product influencing cellular attachment.
Preliminary characterization of naturally occurring temperature-sensitive bovine rotavirus mutants, generated via gene reassortment, indicated that the product of gene segment 10 can influence virus adsorption to MA-104 cells. Cell-free translation of this gene segment in a rabbit reticulocyte system was then utilized to identify a polypeptide with a molecular weight of ca. 21,500 as the primary translation product. This identification provides a basis for further studies of rotavirus assembly and cell interactions which may, in turn, aid in elucidating the replicative strategy of the virus.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D007668:Kidney; D007700:Kinetics; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000302:isolation & purification; D011991:Receptors, Virus / Q000502:physiology; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D010948:Viral Plaque Assay
D010446:Peptide Fragments; D012367:RNA, Viral; D011991:Receptors, Virus