title: umuC-dependent and umuC-independent gamma- and UV-radiation mutagenesis in Escherichia coli.
abstract: The effects of the umuC36 and umuC122::Tn 5 mutations on gamma- and UV radiation mutagenesis (nonsense, missense, and frameshift mutation assays) in Escherichia coli K12 were studied. Although both mutations reduced radiation mutagenesis, the umuC36 mutation appeared to be leaky since considerably more UV radiation mutagenesis could be detected in the umuC36 strain than in the umuC122::Tn 5 strain. In general, the umuC strain showed much larger deficiencies in UV radiation mutagenesis than they did for gamma-radiation mutagenesis. The mutability of the umuC122:: Tn 5 strain varied depending on the radiation dose, and the mutation assay used. For gamma-radiation mutagenesis, the deficiency varied from no deficiency to a 50-fold deficiency; for UV radiation mutagenesis, the deficiency varied from 100-fold to at least 5000-fold. We concluded that both umuC-dependent and umuC-independent modes function for gamma-radiation mutagenesis, while UV radiation mutagenesis seems to depend almost exclusively on the umuC-dependent mode.
mesh_terms: D004260:DNA Repair; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005720:Gamma Rays; D009154:Mutation / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D004251:DNA Transposable Elements