Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens.
We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-micron-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: (1) reduction of background fluorescence, (2) higher resolution of morphologic details, (3) possibility of studying undecalcified bone marrow specimens and (4) possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreover, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D001853:Bone Marrow / Q000201:enzymology / Q000473:pathology*; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007668:Kidney / Q000201:enzymology / Q000473:pathology*; D008198:Lymph Nodes / Q000201:enzymology / Q000473:pathology*; D008867:Microtomy / Q000379:methods; D009195:Peroxidase / Q000032:analysis; D011102:Polyhydroxyethyl Methacrylate; D011109:Polymethacrylic Acids; D013194:Staining and Labeling
D005404:Fixatives; D011109:Polymethacrylic Acids; D011102:Polyhydroxyethyl Methacrylate; D009195:Peroxidase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase