Anti-DNA.RNA sera. Specificity tests and application in quantitative in situ hybridization.
The specificity of anti-nucleic acid antisera can immunocytochemically be evaluated with test systems which apply various nucleic acids immobilized to inert matrices. When using polyrA.polyrU as a model compound for dsRNA, it is important to prevent the formation of the triple stranded form polyrA.(polyrU)2. Anti-dsRNA antibodies which, when tested with the correct test system, proved to be present in an earlier described anti-DNA.RNA serum, could be removed by adsorption. By cytofluorometric comparison of the immuno-fluorescence signals obtained with the anti-dsRNA containing serum and the absorbed serum, it could be shown that the anti-dsRNA antibodies do not contribute to the specific signals measured after in situ hybridization. Repetitive incubations with the anti-DNA.RNA serum led to a considerable increase in immunofluorescence signal.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D008099:Liver / Q000032:analysis / Q000166:cytology; D051379:Mice; D009693:Nucleic Acid Hybridization; D011063:Poly A-U; D012313:RNA / Q000032:analysis* / Q000276:immunology; D012330:RNA, Double-Stranded / Q000032:analysis
D007106:Immune Sera; D012330:RNA, Double-Stranded; D011063:Poly A-U; D012313:RNA; D004247:DNA