Topographical localization of distinct antigenic domains of Toxoplasma gondii with the aid of monoclonal antibodies.
In the present study, a panel of six individual hybridoma derived antibodies produced against the BK strain of Toxoplasma gondii were evaluated for their reactivity in an immunofluorescence test. Each of the six monoclonal antibodies demonstrated a unique pattern of fluorescence localized to distinctive regions on the toxoplasmas. Although all of the six detected antigenic determinants which were shared by at least four different T. gondii strains, monoclonal antibody TG-E4A17 has disclosed hitherto unrecognized population differences among the BK or PH strain parasites. The fact that some of the antigenic molecules are restricted to distinct regions of the toxoplasmas may have implications in the infections process/immune response.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000494:pharmacology; D006825:Hybridomas; D007152:Immunologic Capping; D051379:Mice; D008970:Molecular Weight; D011108:Polymers / Q000494:pharmacology; D013045:Species Specificity; D014122:Toxoplasma / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D011108:Polymers; D005557:Formaldehyde; C003043:paraform