title: [Chronic infection of BHK-21 cells by the Machupo virus. The development of the system and properties of the virus produced by cells of this line].
abstract: A new model of chronic infection of BHK-21 cells with Machupo virus has been obtained. The chronically infected culture of BHK-21-M cells differed morphologically from the control culture by the presence of giant mono- and multinuclear forms with granular cytoplasm and by an increase in the number of small rounded cells. In the system of BHK-21-M cells, the persisting virus was produced permanently but the per cent of infected cells determined by indirect immunofluorescence and the infectious centre method was not high (12-17%). The main factor contributing to the development of Machupo virus persistence in this cell system appears to consist in the formation of defective interfering particles.
mesh_terms: D000818:Animals; D006477:Arenaviruses, New World / Q000276:immunology / Q000472:pathogenicity; D002460:Cell Line; D002908:Chronic Disease; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D006478:Hemorrhagic Fever, American / Q000382:microbiology*; D007668:Kidney; D013696:Temperature; D014762:Viral Interference; D014776:Virus Cultivation; D014779:Virus Replication
vernacular_title: Khronicheskaia infektsiia kletok BHK-21 virusom Machupo. Stanovlenie sistemy i nekotorye svoĭstva virusa, produtsiruemogo kletkmai étoĭ linii.