title: Demonstration of adenosine receptors on mouse cerebral smooth muscle membranes.
abstract: Adenosine receptors have been identified on brain cortical membranes and microvascular preparations. However, they have not been demonstrated on specific microvascular elements in isolation. 2-3H-chloroadenosine was used as a ligand to investigate the presence of adenosine receptors on isolated mouse cerebral smooth muscle membranes. The binding studies reveal the presence of a high affinity binding site with a Kd value of 33.3 nM and a maximal binding capacity (Bmax) of 283 fmol/mg protein. These findings demonstrate that there is an adenosine receptor on cerebral smooth muscle membranes.
mesh_terms: D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000098:blood supply*; D002462:Cell Membrane / Q000032:analysis; D066298:In Vitro Techniques; D051379:Mice; D008833:Microcirculation; D009131:Muscle, Smooth, Vascular / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D011983:Receptors, Purinergic; D014316:Tritium
chemical_list: D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D014316:Tritium; D015762:2-Chloroadenosine; D000241:Adenosine