Metachromatic staining patterns of basic proline-rich proteins from rat and human saliva in sodium dodecyl sulfate-polyacrylamide gels.
A series of basic proteins, rich in proline, were isolated from the salivary secretions of humans and rats. These proteins underwent metachromasia after staining with Coomassie brilliant blue R-250 in sodium dodecyl sulfate-polyacrylamide gels. The technique of destaining gels in several changes of 10% acetic acid after a 30-min staining period is a rapid method of general utility for the identification of proline-rich proteins from total cell lysates from other sources besides saliva.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000032:analysis*; D055232:Proline-Rich Protein Domains; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes; D012463:Saliva / Q000032:analysis; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013045:Species Specificity; D013053:Spectrophotometry / Q000379:methods; D013194:Staining and Labeling; D014316:Tritium
D007202:Indicators and Reagents; D010455:Peptides; D012394:Rosaniline Dyes; D012471:Salivary Proteins and Peptides; D014316:Tritium; D007545:Isoproterenol; C004692:coomassie Brilliant Blue