Neurons from rat forebrain and cerebellum. Isolation and biochemical characterization.
Isolation of neurons from rat forebrain and cerebellum has been performed by a method including trypsin incubation and tissue disaggregation by filtration through successive nylon meshes with a different pore size. Phase contrast microscopy shows highly purified cell preparations. Lactate dehydrogenase, acetylcholinesterase and (Na+-K+) ATPase activities indicate that neurons possess a high cytosolic content and show a good preservation of the plasmatic membrane.
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D002469:Cell Separation; D002531:Cerebellum / Q000166:cytology*; D004027:Diencephalon / Q000166:cytology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D009474:Neurons / Q000032:analysis* / Q000166:cytology / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D013687:Telencephalon / Q000166:cytology*
D007770:L-Lactate Dehydrogenase; D000110:Acetylcholinesterase; D000254:Sodium-Potassium-Exchanging ATPase