Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis.
The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment.
D001407:Bacillus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002439:Cefotaxime / Q000633:toxicity; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000494:pharmacology*; D009154:Mutation; D065093:beta-Lactamase Inhibitors
D002969:Clavulanic Acids; D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; D002439:Cefotaxime