Purification of monoiodinated vasointestinal peptide (M125I-VIP) by high pressure liquid chromatography (HPLC).
In our developed reverse phase high performance liquid chromatography, four forms of M125I-VIP have been isolated free from unlabeled VIP and other iodinated components. The quicker eluting M125I-VIP forms (oxidised and reduced) have a consistently and significantly low non-specific binding with specific target cells of VIP (HT-29) as compared to the late eluting forms of VIP. The retention time is considerably increased when the molecule of VIP is fully iodinated.
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D010084:Oxidation-Reduction; D011956:Receptors, Cell Surface / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D013552:Swine; D014660:Vasoactive Intestinal Peptide / Q000302:isolation & purification* / Q000378:metabolism
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide