Image analysis of FITC-immunofluorescence histochemistry in perivascular substance P-positive nerves.
The ability of image analysis to quantify nerves labelled by FITC-immunofluorescence histochemistry has been tested on whole-mount stretch preparations of perivascular substance P-positive nerves. It has been shown that image analysis can make consistent measurements of nerves demonstrated by immunohistochemistry despite the fading of FITC-fluorescence which occurs during storage and on exposure to ultraviolet light.
D000818:Animals; D002339:Carotid Arteries / Q000294:innervation* / Q000528:radiation effects; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D009474:Neurons / Q000166:cytology*; D013373:Substance P / Q000032:analysis*; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D013373:Substance P; D016650:Fluorescein-5-isothiocyanate