Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells.
Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I was present in the medium during 6 days the cell number increased slightly and there was a substantial, disproportionate rise in alkaline phosphatase activity of the cultures. Thus, IGF I stimulates growth, and in addition, and in contrast to other growth factors, mainly enhances differentiation of osteoblasts.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D010281:Parathyroid Hormone / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D013002:Somatomedins / Q000494:pharmacology*
D007328:Insulin; D010281:Parathyroid Hormone; D010455:Peptides; D013002:Somatomedins; D006003:Glycogen; D000242:Cyclic AMP; D000469:Alkaline Phosphatase