Effect of ACTH 1-17 at different circadian stages on [3H]TdR incorporation into DNA.
The objective was to determine the effect of adrenocorticotropin (ACTH 1-17) on the incorporation of [3H]TdR into DNA (DNA synthesis) in the tongue, esophagus and stomach of CD2F1 mice standardized to 12 hours of light alternating with 12 hours of darkness. A question asked was whether the time of administration along the 24-hour time scale influenced any response found. The response was complex as ACTH 1-17 was capable of bringing about statistically significant increases in the incorporation of [3H]TdR into DNA at certain times, decreases at other times, or no response at still another time. In general the most marked effects of 20 IU/kg of ACTH 1-17 when compared to controls, was to decrease DNA synthesis of as much as 60% 4 hours after administration at the end of the dark or beginning of the light span. A 2- and 3-way analysis of variance supported the conclusion that the kind-of-treatment, time-of-treatment and the interval-to-kill (Sampling time) as well as their interactions are important factors when determining any response of ACTH 1-17 or placebo.
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D003624:Darkness; D004947:Esophagus / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D010446:Peptide Fragments / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism*; D014059:Tongue / Q000378:metabolism*; D014316:Tritium
D010446:Peptide Fragments; D014316:Tritium; D000324:Adrenocorticotropic Hormone; C028238:ACTH (1-17); D013936:Thymidine