title: Potassium activity in cells of isolated perfused cortical thick ascending limbs of rabbit kidney.
abstract: The Na+2Cl-K+ cotransporter in the apical membrane of the cortical thick ascending limb of the Henle's loop (cTAL) of rabbit nephron utilizes the electrochemical gradient for Na+ to transport K+ and Cl- against an unfavorable electrochemical gradient from lumen to cell interior. In the present study attempts are made to measure intracellular K+ activity (a cell K+) under control conditions and after inhibition of the cotransport system by furosemide (50 X 10(-6) mol X l-1). 70 cTAL segments of 55 rabbits were perfused in vitro. Conventional Ling-Gerard and K+-selective microelectrodes were used to measure the PD across the basolateral membrane (PDbl) as well as the PD sensed by the single barrelled K+-selective electrode (PDK+). PDbl was -64 +/- 1 (n = 65) mV and PDK+ + 15 +/- 1 (n = 32) mV under control conditions. The positive PDK+ value, significantly different from zero, indicates that a cell K+ is higher than predicted for passive distribution. The estimate for a cell K+ obtained from PDbl and PDK+ was 113 +/- 8 mmol X l-1. Furosemide lead to the previously reported hyperpolarization of PDbl by 17 +/- 4 (n = 13) mV and to a reduction of PDK+ from 15 +/- 1 to 5 +/- 1 (n = 20) mV. The a cell K+, obtained from this set of data, was 117 +/- 9 mmol X l-1, and was not different from the control value. The present data indicate that a cell K+ is significantly above Nernst equilibrium under control conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism; D004553:Electric Conductivity; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D007684:Kidney Tubules / Q000378:metabolism*; D008138:Loop of Henle / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D004856:Postural Balance / Q000187:drug effects; D011188:Potassium / Q000378:metabolism*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D002712:Chlorides; D005665:Furosemide; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium