[Distribution of various sites of hormonal action between microvessels, glomeruli and tubules isolated from the renal cortex of rabbits].
Collagenase dispersion of rabbit kidney cortex followed by centrifugation on discontinuous sucrose gradient, allowed the simultaneous obtention of microvascular, glomerular and tubular fractions. Microscopic studies and the measurements of cellular renin activity and the muscle-specific-enzyme creatine kinase showed that these vessels were arteriolar in nature and that they contained the preglomerular arterioles. The glomerular or tubular contamination rates were assessed by means of enzymatic markers. AVP stimulated only the tubular fraction whether GTP was present or not. In the absence of GTP, beta-isoproterenol stimulated only the tubular enzyme while in the presence of GTP, the microvascular and especially the glomerular AC were also stimulated. GTP enhanced considerably the microvascular response to bPTH-(1-34) while, in the glomeruli and tubules the effects of GTP and hormones were only additive. The possible physiological significance of these results are discussed.
D000818:Animals; D001160:Arterioles / Q000378:metabolism / Q000502:physiology; D005591:Chemical Fractionation; D007668:Kidney / Q000098:blood supply; D007672:Kidney Cortex / Q000502:physiology*; D007678:Kidney Glomerulus / Q000378:metabolism / Q000502:physiology; D007684:Kidney Tubules / Q000378:metabolism / Q000502:physiology; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism
D011956:Receptors, Cell Surface
Distribution de quelques sites d'action hormonale entre les microvaisseaux, les glomérules et les tubules isolés du cortex rénal de lapin.