Two-dimensional gel electrophoretic separation of the proteins present in chromatin of Escherichia coli.
The polypeptides present in 35S-labelled chromatin prepared from Escherichia coli cells, and polypeptides present in the DNA and RNA complexes obtained by micrococcal nuclease digestion of the chromatin, were analysed by two-dimensional non-equilibrium polyacrylamide gel electrophoresis. Three hundred and thirty-five 35S-labelled polypeptides were detected in the chromatin whereas the DNA- and RNA-containing fractions of the micrococcal nuclease digest contained 126 and 183 polypeptides respectively. The major basic low-molecular-weight polypeptides were found in the DNA-containing fractions.
D001426:Bacterial Proteins / Q000032:analysis*; D002843:Chromatin / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D010779:Photofluorography; D012329:RNA, Bacterial / Q000032:analysis
D001426:Bacterial Proteins; D002843:Chromatin; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial