[Use of R plasmids for isolating Vibrio cholerae donor strains].
Vibrio cholerae and Vibrio eltor donor strains were obtained using R plasmids RP1ts, pRP19.6, pTH10 and pNS10 which carry IS or Tn elements. Resulting from integration of the plasmids into cholera vibrio chromosome, two types of donors were constructed: those resembling Escherichia coli Hfr's and the strains analogous to F+ or F' donors. The order of some chromosome markers were determined in crosses between the donors obtained and V. cholerae recipient strains.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D009154:Mutation; D011815:R Factors; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification*
D004251:DNA Transposable Elements; D005819:Genetic Markers
Ispol'zovanie R-plazmid dlia polucheniia donornykh shtammov kholernogo vibriona.