Cloning of the Bacillus subtilis spoIIA and spoV A loci in phage phi 105DI:1t.
A 6.95 kb HindIII-generated DNA fragment from Bacillus subtilis 168 was inserted into the DNA of phage phi 105DI:1t. The recombinant phage (phi 105DS1) contained DNA of 33.8 kb as compared with 35.2 kb for phi 105DI:1t and 39.2 kb for the wild-type phage. In the presence of helper phage, phi 105DS1 complemented both spoIIA and spoV A mutations in B. subtilis.
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D004587:Electrophoresis, Agar Gel; D005816:Genetic Complementation Test; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D013171:Spores, Bacterial
D004279:DNA, Viral; D004262:DNA Restriction Enzymes