Monoclonal antibodies reveal in blocking reactions the antigenicity and antibody response of poliovirus.
Neutralizing monoclonal antibodies against infectious poliovirus particles, type 1, strain Mahoney, were produced in hybridomas obtained from primed Balb/c mouse lymphocytes fused with the P3/NS-1/1-Ag4-1 mouse myeloma cell line (NS-1). From eleven neutralizing monoclonal antibodies, belonging to four groups differing in their reaction patterns, we chose for our experiments mNt-Abs reacting equally well with infectious poliovirus particles and naturally occurring empty capsids (NEC). We characterized the epitopes of NEC reacting with mNt-Abs by a quantitative absorption (blocking) ELISA with the help of monospecific polyvalent rabbit antisera directed against individual structural poliovirus polypeptides, obtained by two different separation procedures. We ascribe the "neutralizing antigenicity" of NEC to reside in or near polypeptides VP1 and VPO. Furthermore, we were able to demonstrate for the first time for poliovirus, that isolated polypeptides induced blocking antibodies, which were able to prevent binding of neutralizing antibodies to poliovirus NEC.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D000939:Epitopes / Q000276:immunology; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes