[Antigenic properties of the M1 proteins of influenza type B viruses and the detection of a common antigenic determinant of the M1 proteins of type A and B viruses].
Antigenic properties of M1 proteins of influenza B viruses were studied by indirect EIA using monospecific antisera to M1 protein obtained by immunization of rabbits and mice. Antisera with highest titres were obtained by immunization of mice with polyelectrolyte-conjugated M1 protein. Antigenic heterogeneity of M1 proteins of influenza B viruses was demonstrated. M1 proteins of B/Lee/40 and B/USSR/03/84 viruses reacted with B/Hong Kong/72 antiserum to one-fourth of the homologous titre. Cross-experiments using M1 proteins recovered from influenza type A and B viruses and antisera to them revealed heterologous reactions suggesting a common antigenic determinant in A and B M1 proteins. Treatment of the antisera with M1 protein of type A resulted in a decline of antibody titres to M1 proteins of type B. The two available monoclones to M1 protein of type A reacted only with M1 A but not with M1 B.
D000327:Adsorption; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D014763:Viral Matrix Proteins; D014764:Viral Proteins
Antigennye svoĭstva belkov M1 virusov grippa tipa B i vyiavlenie obshcheĭ antigennoĭ determinanty belkov M1 virusov tipa A i B.