Functional role for c-myc in mitogenic response to platelet-derived growth factor.
In BALB/c-3T3 cells, expression of the c-myc gene is stimulated by platelet-derived growth factor (PDGF). Using mouse mammary tumour virus promoter: c-myc recombinant plasmids, 3T3 sublines were constructed in which hydrocortisone was the primary determinant of myc mRNA content. The c-myc gene product is an intracellular mediator of the growth response to PDGF though probably not the only one. Both the human and the mouse c-myc genes stimulate clonal growth of 3T3 cells in PDGF-free medium suggesting new strategies for analysis of oncogenes which do not function in focus formation assays.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004274:DNA, Recombinant / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009857:Oncogenes / Q000187:drug effects*; D010957:Plasmids; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D018139:Simplexvirus / Q000235:genetics; D014162:Transfection
D004274:DNA, Recombinant; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D006854:Hydrocortisone