Growth characteristics of murine tumors: neuroblastoma, renal cell adenocarcinoma, Wistar/Furth Wilms' after storage in a cryopreservation system.
Three murine tumor models, neuroblastoma CL 300, renal cell adenocarcinoma, and Wistar/Furth Wilms' tumor were evaluated for long-term storage in a cryopreservation system. Several transfer generations were observed in all tumor groups. Percent of tumor take poststorage, rate of tumor growth postimplantation and percent of metastatic development were calculated. Tumor take ranged from 5 to 100% poststorage and implant. Tumor growth was noted to require more days with stored tumor than with tumors implanted directly from donor to recipient. Tumor weights were comparable. Metastatic growth was observed in all transfer groups implanted with renal cell adenocarcinoma. Metastatic growth was not observed in all Wistar/Furth Wilms' transfer groups nor in all the neuroblastoma CL 300 transfer groups. This cryopreservation system can be utilized for storing tumor cells with up to 100% recovery of the tumor cell growth.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D005615:Freezing; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D013997:Time Factors; D014021:Tissue Preservation / Q000295:instrumentation*; D009396:Wilms Tumor / Q000473:pathology*