title: Linkage map of two HLA-SB beta and two HLA-SB alpha-related genes: an intron in one of the SB beta genes contains a processed pseudogene.
abstract: Three overlapping cosmid clones contain coding sequences for four HLA Class II genes, provisionally identified as two HLA-SB alpha and two HLA-SB beta genes. The genes are in the order beta, alpha, beta, alpha, inverted with respect to each other. One of the SB beta genes contains a 513 bp sequence that appears to be a processed pseudogene, flanked by direct 17 bp repeat sequences, in the intron upstream of the beta 1 exon. The pseudogene is homologous to a family of sequences of approximately 25-40 members, most of which are not on chromosome 6. A cDNA clone, highly homologous to the pseudogene, except for its 5' end, contains a normal poly(A) addition site and a poly(A) tail. The cDNA clone is homologous to a single-copy gene in both man and mouse, encoded on human chromosome 15. A search of published DNA sequences identified a mouse sequence, with about 77% similarity to the pseudogene sequence, in the negative strand of an intron in a mouse dihydrofolate reductase gene. The second SB beta gene does not contain the pseudogene sequence.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002901:Chromosomes, Human, 13-15; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005796:Genes; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D006682:HLA-DP Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
chemical_list: D006682:HLA-DP Antigens; D000949:Histocompatibility Antigens Class II; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes