title: Serum-free medium for generation and propagation of functional human cytotoxic and helper T cell clones.
abstract: A serum-free lymphocyte culture medium is described in which serum is replaced by bovine serum albumin, transferrin, insulin, ethanolamine and a mixture of saturated and unsaturated fatty acids (linoleic acid, oleic acid and palmitic acid). In this serum-free medium proliferative and cytotoxic responses induced in mixed lymphocyte culture were comparable with those obtained in medium containing serum. Antigen-specific cytotoxic and helper T cells were isolated and could be propagated in serum-free medium without loss of function.
mesh_terms: D001790:Blood Physiological Phenomena; D002460:Cell Line; D002469:Cell Separation; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000276:immunology; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D003470:Culture Media