Stimulant effects of W-7, a calmodulin-antagonist, on renin release from rat kidney cortical slices.
Effects of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin-antagonist, on renin release was examined using rat kidney cortical slices. Although the amounts of renin release from the slices were stable during incubation of 3 consecutive 15 min periods in Krebs-Ringer bicarbonate solution (pH 7.4), the addition of W-7 to the incubation medium resulted in a concentration-dependent increase in renin release from the slices. The stimulant effect of W-7 on renin release was abolished by the removal of calcium from the incubation medium. These results suggest that the inhibition of the calcium-calmodulin system leads to the stimulation of renin release from rat kidney cortical slices.
D000818:Animals; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D000242:Cyclic AMP / Q000502:physiology; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology*
D002147:Calmodulin; D013449:Sulfonamides; C017967:W 7; D000242:Cyclic AMP; D012083:Renin; D002118:Calcium