The time course of GABA action on the crayfish stretch receptor: evidence for a saturable GABA uptake.
The conductance increase induced by bath application of GABA has been measured in voltage-clamped stretch-receptor neurones of crayfish. A rapid conductance increase was obtained only at GABA concentrations above 3.3 X 10(-4) M. The response to lower GABA concentrations (between 10(-4) and 10(-6) M) developed slowly over 30-60 min. Repetitive application of intermediate GABA concentrations induced postsynaptic conductance changes which were progressively enhanced in their onset and magnitude. In the presence of nipecotic acid or in Na+-free Ringer solutions, the response to all GABA concentrations was rapid and constant for each concentration. The time course of inhibitory postsynaptic currents was unaffected by nipecotic acid. These results suggest the presence of a saturable GABA uptake system which limits the access of bath-applied GABA to postsynaptic receptors. This system has little if any effect on the termination of response to synaptically released GABA.
D000818:Animals; D003400:Astacoidea; D004305:Dose-Response Relationship, Drug; D008297:Male; D008465:Mechanoreceptors / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000294:innervation*; D009433:Neural Inhibition / Q000187:drug effects; D009557:Nipecotic Acids / Q000494:pharmacology; D011392:Proline / Q000031:analogs & derivatives*; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D011963:Receptors, GABA-A; D013569:Synapses / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects*; D014649:Vas Deferens / Q000294:innervation; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000494:pharmacology*
D009557:Nipecotic Acids; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; C030278:nipecotic acid; D005680:gamma-Aminobutyric Acid; D011392:Proline; C010983:homoproline