Purification of calmodulin from bovine parotid gland.
Calmodulin was purified from bovine parotid gland. This calmodulin showed a single band upon sodium dodecyl sulfate polyacrylamide gel electrophoresis, and a characteristic decrease of the mobility on native gel in the presence of Ca2+. The parotid gland calmodulin activated also calmodulin-deficient cyclic nucleotide phosphodiesterase in a Ca2+-dependent fashion, and this activation was abolished by trifluoperazine, W-7 or chlorpromazine. These results may indicate clearly the existence of calmodulin in bovine parotid gland.
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000302:isolation & purification; D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000302:isolation & purification*; D002417:Cattle; D002746:Chlorpromazine / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D010306:Parotid Gland / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D011506:Proteins; D014268:Trifluoperazine; D004533:Egtazic Acid; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D002118:Calcium; D002746:Chlorpromazine