Heterogeneity of 3' nontranslated regions in proopiomelanocortin (POMC) precursor mRNA of chum salmon Onchorynchus keta: polymorphism of the gene.
Heterogeneity of salmon pituitary proopiomelanocortin (POMC) mRNA was shown by comparison of the nucleotide sequence of independently isolated cDNA clones encoding POMC, pSSM90, pSSM53 and pSSM17, the last of which was previously characterized. Newly isolated clones pSSM90 and pSSM53 contained inserts of 1228 and 666 base pairs, respectively (excluding poly(A)). Sequence analysis revealed that the former contained sequences coding for the carboxy half of putative corticotropin (ACTH), the whole region of beta-lipotropin (beta-LPH), and the entire 3' nontranslated region, while the latter contained only the 3' nontranslated region. Sequence comparison of the three clones revealed that there are some definite nucleotide changes in the 3' nontranslated regions, i.e., base replacements and base additions at multiple sites, whereas no single change was observed in the coding regions, thus demonstrating heterogeneity and hence polymorphism of the gene in the salmon genome.
D000324:Adrenocorticotropic Hormone / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005796:Genes; D010908:Pituitary Hormones, Anterior / Q000235:genetics*; D011110:Polymorphism, Genetic; D011333:Pro-Opiomelanocortin; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012474:Salmon; D008083:beta-Lipotropin / Q000235:genetics*
D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D004247:DNA; D008083:beta-Lipotropin; D004262:DNA Restriction Enzymes