Three subunit proteins of membrane enzymes in mitochondria of Neurospora crassa contain a pantothenate derivative.
Three proteins of the inner mitochondrial membrane of Neurospora crassa were found to be covalently modified with a derivative of pantothenic acid. One of these proteins is a subunit of cytochrome c oxidase and two are subunits of the ATPase-ATP synthase. Cells of a pantothenate auxotroph of N. crassa were labeled with [14C]pantothenic acid, and mitochondrial proteins containing radiolabeled pantothenate were detected by electrophoresis of detergent-solubilized mitochondria. Mitochondria from cells that were colabeled with [14C]pantothenate and [3H]leucine were reacted with specific antisera against the cytochrome c oxidase and F1-ATPase enzyme complexes. Electrophoresis of the labeled subunits of these isolated complexes showed that the [14C]pantothenate-associated peptides corresponded to [3H]leucine-labeled subunit 6 of cytochrome c oxidase and two [3H]leucine-labeled subunits (tentatively identified as subunits 8 and 11) of the ATPase-ATP synthase. Pantothenate modification of these enzyme subunits, which are synthesized on extramitochondrial ribosomes, may contribute to their transport and assembly into mitochondria, or it may participate in the catalytic activity of the assembled enzymes.
D025181:ATP Synthetase Complexes; D000251:Adenosine Triphosphatases / Q000032:analysis; D002250:Carbon Radioisotopes; D003576:Electron Transport Complex IV / Q000032:analysis; D007425:Intracellular Membranes / Q000201:enzymology*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000032:analysis*; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000032:analysis; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D010770:Phosphotransferases / Q000032:analysis
D002250:Carbon Radioisotopes; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D010205:Pantothenic Acid; D003576:Electron Transport Complex IV; D010770:Phosphotransferases; D025181:ATP Synthetase Complexes; D000251:Adenosine Triphosphatases