Cytolytic T cells recognize the two amino-terminal domains of H-2 K antigens in tandem in influenza A infected cells.
We have genetically engineered three alleles of the K locus of the major histocompatibility complex (MHC) of the mouse. These novel hybrid H-2K genes were introduced into mouse 1T 22-6 cells (H-2q), and their products were shown to be expressed on the cell surface. The hybrid H-2 K antigens were examined for their ability to function as restricting elements for cytotoxic T lymphocytes during influenza A infection. Both the alpha 1 and alpha 2 domains of the Kd antigen were required for T cell recognition. This implies an important role for "conformational determinants" on H-2 antigens acting as restricting elements. The cytoplasmic domain of the Kb antigen is not phenotypically important for recognition by T cells.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D006183:H-2 Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
D006183:H-2 Antigens; D004262:DNA Restriction Enzymes