Role of HLA class II products in proliferative T-lymphocyte responses to PPD. Evidence of a regulatory influence associated with MB1.
HLA class II determinants were analysed for their role in monocyte-T-cell interactions in the proliferative response to purified protein derivative (PPD). Allogeneic cell combinations of monocytes and T cells were tested with a range of suboptimal PPD concentrations. For each cell combination tested, a summary measure to characterize the antigen-induced response curve was calculated by means of regression analysis of the response to the dose of PPD added, thus obviating a further need to correct for mixed lymphocyte reactivity. In 200 distinct monocyte-T-cell combinations, HLA-DR appeared to be the major restricting element. Additionally, a marginal influence of HLA-MB or -MT matching was observed and no evidence of HLA-SB-restricted responses. Remarkably, the HLA-MB1 specificity was significantly associated with low responsiveness in DR sharing monocyte-T-cell combinations, indicating a modulating role of MB1 in DR-restricted T-cell responses.
D000939:Epitopes; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D014373:Tuberculin