Isolation, characterization, and chromosome assignment of mouse N-ras gene from carcinogen-induced thymic lymphoma.
Treatment of mice with the carcinogen N-methylnitrosourea results in the development of thymic lymphomas with frequent involvement of the N-ras oncogene. The activated mouse N-ras gene was isolated from one of these lymphomas and, by transformation in concert with restriction digestion, a map of the gene was prepared and its approximate boundaries were determined. By means of somatic cell hybrids the normal N-ras gene was found to be unlinked to other members of the ras gene family.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D008040:Genetic Linkage; D006822:Hybrid Cells; D008223:Lymphoma / Q000139:chemically induced / Q000235:genetics*; D008770:Methylnitrosourea; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes; D013953:Thymus Neoplasms / Q000139:chemically induced / Q000235:genetics*
D008770:Methylnitrosourea; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI