Monoclonal antibody against an antigen selectively assembled into vesicular stomatitis virus virions from HeLa cells.
A mouse hybridoma cell line IIB9, secreting IgG2b antibody specific for a HeLa cell antigen, was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with purified VSV tsO45 mutant (defective in assembly of G protein) which had been reproduced at a non-permissive temperature in HeLa cells. The monoclonal antibody IIB9 was strictly specific for HeLa cells in two tests: (1) reaction with VSV or Chandipura virus phenotypically mixed with host cell antigen, (2) complement-dependent cytotoxicity test (51Cr-release).
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D003673:Defective Viruses / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D006367:HeLa Cells / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm