A simple computer program for Scatchard plot analysis of hormone receptors including statistical analysis on a low cost desk top calculator.
A simple procedure for the estimations of standard errors in hormone binding parameters derived from Scatchard analysis is proposed by assuming an univariate linear regression statistic model. The complete set of necessary formulae is presented and a practical way for calculation is solved using the binding of human growth hormone to rat adrenal membranes, as a practical example. The comparison between the results obtained by the method proposed and by using a well proved and accepted computer program, shows unequivocally that the differences are practically undistinguishable. The complete program to calculate the Scatchard parameters and their statistical analysis is available on request.
D001665:Binding Sites; D003201:Computers; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012044:Regression Analysis; D012984:Software
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface