Microneutralization test in PK(15) cells for assay of antibodies to louping ill virus.
A microneutralization test in PK(15) cells was developed to measure the neutralizing antibody response of a group of ponies experimentally challenged with louping ill virus. Viral cytopathic effect was maximal after 6 days of incubation, at which point titration endpoints were clear-cut and readily determinable. The assay compared favorably with the mouse neutralization test for accuracy and ease of performance.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D009500:Neutralization Tests
D000914:Antibodies, Viral