[Effect of experimental diabetes and its treatment with insulin on the translation of RNAs and protein synthesis in rat enterocytes].
We examined the effect of diabetes on protein synthesis in intestinal epithelial cells. Isolated enterocytes of diabetic rats display a marked decrease (50%) in 3H-leucine incorporation into intestinal proteins as compared to normal cells. Treatment of diabetics with insulin restores the level of protein synthesis up to normal values. Similarly, in vitro translation products of isolated RNA are much lower in diabetics than in normal or treated diabetics. It is concluded that in intestinal cells, insulin can play an important role in regulating the biosynthesis of proteins by modulating the translation of specific RNAs.
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology* / Q000627:therapeutic use; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012313:RNA / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D012313:RNA; D008715:Methionine; D007930:Leucine
Effet du diabète expérimental et de son traitement à l'insuline sur la traduction des RNAs et la synthèse protéique dans les entérocytes de rat.