Enzymes of nucleic acid metabolism as biochemical markers of T cell development in mouse.
The levels of three purine salvage enzymes, adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and 5'-nucleotidase (5'-N), which are known to be associated with certain immunodeficiency disorders were determined in mouse T lymphocytes. These enzymes showed characteristic changes depending on the stage of T cell development. The activity of ADA was 5-fold higher in thymocytes compared to splenic T cells. On the other hand, the splenic T cells displayed a 2-fold and a 4-8 fold greater activity of PNP and 5'-N, respectively than those of thymocytes. The apparent Km and Vmax values have been determined for all the 3 enzymes in the immature and mature T cells. The data demonstrate that the absolute and relative activity of these enzymes may be used as biochemical markers to characterize the T lymphocytes during different stages of differentiation.
D015720:5'-Nucleotidase; D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D007700:Kinetics; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D009700:Nucleoside Deaminases / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism*; D010430:Pentosyltransferases / Q000378:metabolism*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000201:enzymology; D013950:Thymus Gland / Q000166:cytology
D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase