A new method in criminology: use of ELISA to detect AFP on different materials with monoclonal anti-alpha-fetoprotein.
A new method has been introduced to distinguish normal adult serum stains from fetal or newborn serum and amniotic fluid stains with ELISA in cases of criminal abortion and infanticide. The method is based on the sensitive detection of alpha-fetoprotein (AFP) by a two-site enzyme immunoassay (EIA) following its elution with high efficiency from different materials (e.g., cotton, paper, synthetic fabric, or glass) by phosphate-buffered 0.5 M NaCl solution.
D000328:Adult; D000653:Amniotic Fluid / Q000032:analysis; D000911:Antibodies, Monoclonal; D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D011247:Pregnancy; D000509:alpha-Fetoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000509:alpha-Fetoproteins