Role of cell division in differentiation of myoblasts infected with a temperature-sensitive mutant of Rous sarcoma virus.
The relationship between a potential requirement for cell DNA synthesis and the expression of differentiated muscle cell functions was investigated using primary chicken embryo myoblasts infected with a temperature-sensitive mutant of Rous sarcoma virus (RSV). Under optimized conditions, transformed myoblasts growing at the permissive temperature could differentiate into multinucleated myotubes, express muscle-specific myosin, desmin and acetylcholine receptors in the absence of DNA synthesis and cell division following a shift to the non-permissive temperature. Furthermore, the experiments demonstrate that individual RSV-infected myoblasts have two options: either to divide and express the transformed phenotype or to withdraw from the cell cycle and differentiate into myotubes. The choice between these options appears to depend on the protein-kinase activity of pp60src, the src gene product.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D004261:DNA Replication; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity; D009132:Muscles / Q000196:embryology* / Q000502:physiology; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D011494:Protein Kinases / Q000235:genetics; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics
D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D014764:Viral Proteins; D016685:Mitomycin; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)