Characterization and carcinogen sensitivity of an established endothelial-like cell line derived from adult rat liver tissue.
To define the in vitro response of nonparenchymal liver cells to chemical carcinogens, an endothelial-like cell line (RLC-1) derived from adult rat hepatic tissue was established in long-term culture. The RLC-1 line exhibited morphologic, ultrastructural and cytoskeletal characteristics consistent with derivation from mesenchymal vascular elements within the liver. Using various criteria to monitor changes in growth properties, RLC-1 cells were determined to be resistant to transformation by diethylnitrosamine, partially transformed by benzo[a]pyrene, and completely transformed by methylazoxymethanol acetate and N-methyl-N'-nitro-N-nitrosoguanidine. The RLC-1 cell system provides a means to assess potential effects of procarcinogens, as well as intrinsically reactive compounds, on an isolated nonparenchymal liver cell population.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000494:pharmacology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure*; D004052:Diethylnitrosamine / Q000494:pharmacology; D005434:Flow Cytometry; D005779:Immunodiffusion; D008099:Liver / Q000166:cytology*; D008746:Methylazoxymethanol Acetate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D001580:Benzopyrenes; D008769:Methylnitronitrosoguanidine; D001564:Benzo(a)pyrene; D004052:Diethylnitrosamine; D008746:Methylazoxymethanol Acetate