Inhibition of the mutagenicity of benzo[a]pyrene in the V79/HGPRT system by bioantioxidants.
In the presence of metabolic activation (S9 microsomal fraction of mouse-liver homogenate) the mutagenicity of benzo[a]pyrene (BP) in Chinese hamster V79 cells was inhibited by the phenolic bioantioxidants (BA) Dibunol (2,6-di-tert-butyl-4-methylphenol-D) and 5-methylresorcine(5-MR). The mixture BP + D and BP + 5-MR at molar ratios of 1:1 and 1:85 respectively showed no mutagenic activity compared to the control. One can assume that D and 5-MR inhibited BP-induced mutagenesis by binding the free radicals of BP metabolites with the formation of less active phenolic derivatives and also by linkage with cytochrome P-450, which prevents further metabolic activation of BP.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000633:toxicity*; D001711:Biotransformation; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007202:Indicators and Reagents / Q000494:pharmacology*; D008168:Lung; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D012118:Resorcinols / Q000494:pharmacology*
D000975:Antioxidants; D001580:Benzopyrenes; D007202:Indicators and Reagents; D009153:Mutagens; D012118:Resorcinols; D002084:Butylated Hydroxytoluene; D001564:Benzo(a)pyrene; C005282:orcinol; D007041:Hypoxanthine Phosphoribosyltransferase