Ultrastructure of bony-fish heart tissue treated with beta-glycerophosphate/lead citrate or adenosine triphosphate/lead nitrate.
The ultrastructure of bony-fish heart tissue is described when subjected to beta-glycerophosphate/lead citrate (9.2 less than or equal to pH less than or equal to 9.4) or adenosine triphosphate/lead nitrate (pH = 7.3). When treated in the former way, electron dense precipitates occur in the endocardial and myocardial endoplasmic/sarcoplasmic reticulum, Golgi apparatus, and nuclear intermembranous space. Such precipitates are absent in the control tissue and reflect therefore probably alkaline phosphatase activity. In tissue treated with adenosine triphosphate/lead nitrate the precipitates are irregularly distributed, and may also occur in the control tissue. In the present material, they therefore seem not to reflect adenosine triphosphatase activity. The results are compared and discussed with those revealed in the present study for the bony-fish intestine and with those previously reported for various mammalian tissues.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D005399:Fishes / Q000033:anatomy & histology*; D005994:Glycerophosphates; D006651:Histocytochemistry; D007854:Lead; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure*; D013194:Staining and Labeling
D005994:Glycerophosphates; D007854:Lead; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; C031463:beta-glycerophosphoric acid