A screening assay for the tetrachlorodibenzo-p-dioxin receptor using the [125I]iodovaleramide derivative of trichlorodibenzo-p-dioxin as the binding ligand.
A relatively simple assay method for the putative cytosolic 'receptor' that binds 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds is described. The assay is based on specific binding of [125I]dioxin to cytosol 'receptor' protein. Saturation is ensured by competition experiments in which unlabeled TCDD and other competitors displace the radiolabeled ligand from specific binding sites. This assay has been applied to estimation of levels of 'receptor' in cytosol.
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D004147:Dioxins / Q000378:metabolism; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000378:metabolism*
D004147:Dioxins; D007457:Iodine Radioisotopes; D000072317:Polychlorinated Dibenzodioxins; D011506:Proteins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; C042433:1-N-5-iodovaleramido-3,7,8-trichlorodibenzo-4-dioxin