Early kinetics of Ca2+ fluxes and histamine release in rat mast cells stimulated with compound 48/80.
The kinetics of Ca2+ uptake and efflux have been measured in rat peritoneal mast cells stimulated with compound 48/80 using rapid mixing and a silicone oil centrifugation technique. Responses at one-second time intervals were resolved beginning as early as three seconds after initial stimulation. The results clearly demonstrate that Ca2+ uptake occurs after the initiation of histamine release. Ca2+ efflux occurs simultaneously with histamine release. The implications of these findings are discussed and the technique is described.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D006636:Histamine Release / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence / Q000379:methods; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D002132:Calcium Radioisotopes; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium