The cellulase of Trichoderma viride. Separation of the components involved in the solubilization of cotton.
1. Culture filtrates from Trichoderma viride have been fractionated by gel filtration on Sephadex G-75 followed by ion-exchange chromatography on DEAE- and SE-Sephadex. 2. The components essential for attack on cotton are a carboxymethylcellulase, a cellobiase and a third (C(1)) component which has no action on CM-cellulose, cellobiose or cotton. 3. These components, which together can completely convert cotton into water-soluble products, lose this ability when separated and regain it quantitatively when recombined in their original proportions.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005959:Glucosidases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D003368:Gossypium / Q000378:metabolism*; D008747:Methylcellulose; D003904:Mitosporic Fungi / Q000201:enzymology*; D012995:Solubility
D008747:Methylcellulose; D005959:Glucosidases; D006026:Glycoside Hydrolases