Evidence for a similar receptor site for binding of [3H] leukotriene E4 and [3H] leukotriene D4 to the guinea-pig crude lung membrane.
To determine whether the action of leukotriene E4 is mediated by its cross reaction with leukotriene D4 receptor sites, we compared [3H] leukotriene E4 and [3H]leukotriene D4 binding activities in selected tissues as well as their competition results in the guinea-pig crude lung membrane. We demonstrated good correlation of [3H]leukotriene E4 and [3H]leukotriene D4 binding activities among the tissues studied. A significant correlation was demonstrated between the ability of leukotriene C4, D4 and E4, FPL-55712 and arachidonic acid to inhibit lung [3H]leukotriene E4 and [3H]leukotriene D4 binding. These correlations suggest that leukotriene E4 binds to a site which is similar to or close to the leukotriene D4 receptor.
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D006168:Guinea Pigs; D007700:Kinetics; D017999:Leukotriene E4; D008168:Lung / Q000378:metabolism*; D009928:Organ Specificity; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018077:Receptors, Leukotriene; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism*; D013045:Species Specificity
D011956:Receptors, Cell Surface; D018077:Receptors, Leukotriene; D013189:SRS-A; D017999:Leukotriene E4