A bacteriophage T5 mutant with an increased frequency of single-chain interruptions.
A T5 mutant whose DNA has two sites where single-chain interruptions occur with a higher frequency than in wild-type DNA was isolated. Both sites occurred within the same strand as do the natural interruptions in T5 DNA, and both were due to alteration of the mechanism that generates the interruptions.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D013045:Species Specificity; D013604:T-Phages / Q000235:genetics*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004262:DNA Restriction Enzymes