The human lymphocyte micronucleus assay. Response of cord blood lymphocytes to gamma-irradiation and bleomycin.
The induction of micronuclei in human cord blood lymphocytes by treatment with gamma-irradiation and bleomycin has been measured. Culture durations which gave peak MN frequencies were determined. The lowest tested doses, 0.1 Gy irradiation and 1.25 micrograms/ml bleomycin, produced significant increases in the frequency of micronuclei. The spontaneous frequency of micronucleated lymphocytes in 28 cord blood samples ranged between 0.5 and 9.5 per thousand lymphocytes, with a modal value of 2.5. The method is evaluated for its potential usefulness in monitoring populations for chromosome breakage.
D001761:Bleomycin / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005312:Fetal Blood; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects* / Q000528:radiation effects
D001761:Bleomycin