The detection of distinct epitopes of human alpha-fetoprotein (AFP) by solid phase radioimmunoassay using hybridoma culture fluid.
A solid phase radioimmunoassay was developed for the epitope analysis of human alpha-fetoprotein (AFP) using hybridoma culture fluids. The following incubation sequence was performed: anti-mouse Ig, hybridoma culture fluid, normal mouse serum, and a mixture of 125I-labeled AFP and hybridoma culture fluid. Seven epitopes were detected by monoclonal antibodies produced by 15 presumably independent hybridoma clones.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D006825:Hybridomas; D051379:Mice; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000509:alpha-Fetoproteins