Effect of pH on reactivity of monoclonal antibodies to Chlamydia.
The influence of pH on the reactivity of anti-Chlamydia monoclonal antibodies with genus-, species- and type-specific activity to chlamydial elementary body (EB) antigens was studied by solid-phase enzyme immunoassay. The antibodies bound readily to EB antigen from pH 4 to 9, but had a reduced binding efficiency at more extreme pH conditions. Antibodies although having a wide pH range of optimal binding, bound non-specifically at acidic pH (4-6). This non-specific binding was not seen with polyclonal guinea pig or rabbit anti-Chlamydia antibodies. Thus it seems to be a specific property of mouse immunoglobulins when tested against cell antigen.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial / Q000032:analysis*; D002689:Chlamydia / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D000939:Epitopes