The localization of the gene for apolipoprotein C-II to chromosome 19.
Human apolipoprotein (apo) C-II, a 79 amino acid protein, functions as a cofactor for lipoprotein lipase, the enzyme which catalyzes the hydrolysis of plasma triglycerides. The chromosomal location of apoC-II has been determined by filter hybridization analysis of human-mouse hybrid cells. Southern blots of DNA from 21 human-mouse hybrid cells were hybridized with a 190 base pair nick translated probe prepared from a Hinf I digest of an apoC-II cDNA clone. Without exception, ApoC-II segregated with chromosome 19 thus establishing synteny with the apoE and LDL receptor genes known to be localized to this chromosome. The localization of the apoC-II gene to chromosome 19 will permit more detailed analysis of the genomic organization and linkages of the apolipoprotein genes.
D000818:Animals; D053304:Apolipoprotein C-II; D001053:Apolipoproteins / Q000235:genetics*; D001056:Apolipoproteins C; D001483:Base Sequence; D002874:Chromosome Mapping; D002903:Chromosomes, Human, 19-20; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005347:Fibroblasts / Q000378:metabolism; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D010957:Plasmids
D053304:Apolipoprotein C-II; D001053:Apolipoproteins; D001056:Apolipoproteins C; D004247:DNA; D004262:DNA Restriction Enzymes