The isolation of prothrombin, Factor IX and Factor X from human Factor IX concentrates.
A relatively simple and reproducible procedure is described for the isolation of functionally and electrophoretically homogeneous prothrombin, factor IX and factor X from clinical Factor IX Concentrates. The procedure involves ammonium sulphate fractionation; then chromatography on DEAE-Sephadex A50, dextran sulphate-Sepharose and heparin-Sepharose. High recovery of all three procoagulants was obtained: 32% for factor IX; 32% for factor X; 29% for prothrombin.
D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D016264:Dextran Sulfate; D003911:Dextrans; D005164:Factor IX / Q000302:isolation & purification*; D005170:Factor X / Q000302:isolation & purification*; D006801:Humans; D011516:Prothrombin / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D003911:Dextrans; C037405:heparin-sepharose; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X; D012685:Sepharose; D016264:Dextran Sulfate