title: Structure of the terminal reducing heptasaccharide of polysaccharide 1 isolated from the Bordetella pertussis endotoxin.
abstract: The tetrasaccharide beta-D-glucopyranosyl-(1,3)-beta-D-glucopyranuronyl-(1, 2)-L-glycero-alpha-D-manno-heptopyranosyl-(1,5)-3-deoxy-D-manno-2- octulosonic acid was isolated after treatment of polysaccharide 1 of Bordetella pertussis endotoxin with nitrous acid. Taking into account previously identified di- and trisaccharide fragments and analytical data obtained for the intact polysaccharide 1, we present the structure of a heptasaccharide that is thought to represent the region immediately adjacent to the hydrophobic (lipid A) moiety of lipopolysaccharide 1 of the B. pertussis endotoxin. This heptasaccharide represents 50 to 60% of the complete polysaccharide structure.
mesh_terms: D001886:Bordetella pertussis / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004731:Endotoxins / Q000302:isolation & purification*; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D004731:Endotoxins; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial