title: Factors influencing hematopoietic spleen colony formation in irradiated mice. I. The normal pattern of endogenous colony formation.
abstract: Data pertaining to the endogenous mouse spleen colony system, 10 days postirradiation, are presented. The D(o) for visible colonies is 78 R, while that for 6 hr iron uptake over a range of 600-800 R is 50 R. The D(o) for spleen weight is 196 R and that for IUdR uptake is 193 R. These measurements increase with the age of the mouse. Hypertransfusion decreases spleen iron uptake and colony number. DF-(32)P and sodium sulfate-(35)S are not useful indicators of splenic hematopoiesis in this system. Visible hematopoietic colonies in the spleen are not produced by vinblastine, nitrogen mustard, methotrexate, or cyclophosphamide.
mesh_terms: D000367:Age Factors; D000818:Animals; D001803:Blood Transfusion; D003036:Cobalt Isotopes; D006410:Hematopoiesis / Q000528:radiation effects*; D007501:Iron / Q000378:metabolism; D007531:Isoflurophate / Q000378:metabolism; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D009929:Organ Size; D011833:Radiation Injuries, Experimental; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D013431:Sulfates / Q000378:metabolism; D013460:Sulfur Isotopes; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D003036:Cobalt Isotopes; D009588:Nitrogen Mustard Compounds; D013431:Sulfates; D013460:Sulfur Isotopes; D007531:Isoflurophate; D014747:Vinblastine; D007501:Iron; D008727:Methotrexate