Steroidogenic effect of exogenous phospholipase C on bovine adrenal fasciculata cells.
Phospholipase C (Bacillus cereus) added to the incubation medium stimulated the steroidogenic activity of bovine adrenal zona fasciculata cell suspensions to a level similar to that induced by optimal concentration of ACTH. This effect was not related to an increase of cyclic AMP; it was calcium-dependent and was also induced by an other bacterial phospholipase C (from Clostridium perfringens) whereas phospholipases A2 and D were ineffective. Phospholipid metabolism was examined in these cells after radiolabeling with [14C]-glycerol or [32P]orthophosphate. Phospholipase C induced a very fast (5 seconds) increase in cellular [14C]-1,2-diacylglycerol followed by [32P] labeling of phosphatidic acid and phosphatidylinositol. These events preceded the stimulation of steroidogenesis which was detectable after 2 minutes of incubation. These observations suggest that activation of an endogenous phospholipase C activity may be considered as an early event in the response of bovine adrenocortical cells to steroidogenic effectors such as angiotensin II and acetylcholine.
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D006854:Hydrocortisone / Q000096:biosynthesis*; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis; D010740:Phospholipases / Q000494:pharmacology*; D010743:Phospholipids / Q000096:biosynthesis*; D010738:Type C Phospholipases / Q000494:pharmacology*
D004075:Diglycerides; D010710:Phosphates; D010716:Phosphatidylinositols; D010743:Phospholipids; D000242:Cyclic AMP; D010740:Phospholipases; D010738:Type C Phospholipases; D002118:Calcium; D006854:Hydrocortisone