Two different aldolase A mRNA species in rat tissues.
Double-stranded DNA was synthesized with reverse transcriptase from size-fractionated poly(A)-containing RNA from rat ascites hepatoma cells. The cDNA was introduced into Escherichia coli HB101 using pBR322 DNA as a cloning vector. Several plasmids containing aldolase A cDNA were identified by colony hybridization with 32P-labeled cDNA prepared from immunologically purified aldolase A mRNA. The partial amino acid sequence of the cDNA sequence was determined, and found to coincide with that of rabbit aldolase A. Using aldolase A cDNA as a hybridization probe, the aldolase A mRNA concentrations in various rat tissues were analysed, and two aldolase A mRNA species differing in nucleotide length were found; the smaller mRNA (about 1550 nucleotides) in muscle, and the larger one (about 1650 nucleotides) in brain and hepatoma cells.
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002874:Chromosome Mapping; D004247:DNA / Q000096:biosynthesis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis / Q000235:genetics*; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D005634:Fructose-Bisphosphate Aldolase