Restriction site bank vectors. II. DNA sequence analysis of plasmid pJRD158.
pJRD158 is a small plasmid vector (3903 bp) derived from pBR327 and specifying resistance to ampicillin and tetracycline. It contains 28 unique restriction sites (and 4 nonunique restriction sites) that can be used for cloning. The DNA sequence and computer-assisted restriction site analysis of pJRD158 are reported. Evidence is also presented that suggests a 2-bp revision of the DNA sequence of pBR322 in the RNA primer region.
D000667:Ampicillin / Q000633:toxicity; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000187:drug effects; D005822:Genetic Vectors; D010403:Penicillin Resistance; D011815:R Factors; D013752:Tetracycline / Q000633:toxicity
D000667:Ampicillin; D004262:DNA Restriction Enzymes; D013752:Tetracycline