A kinetic isotope dilution assay for glycerol.
Using glycerol kinase and [3H]glycerol, a kinetic isotope dilution assay for glycerol has been developed. Reactant and product are separated by stepwise elution from QAE-Sephadex. This assay is sensitive to as little as 100 pmol of glycerol, avoids numerous drawbacks of the traditional fluorescent assay, and readily detects glycerol production by fewer than 10(5) cardiomyocytes.
D002852:Chromatography, Ion Exchange; D005990:Glycerol / Q000032:analysis*; D005991:Glycerol Kinase; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism; D010770:Phosphotransferases; D011865:Radioisotope Dilution Technique
D010770:Phosphotransferases; D005991:Glycerol Kinase; D005990:Glycerol