title: Two cytosolic, Ca2+-dependent, neutral proteinases from rabbit liver: purification and properties of the proenzymes.
abstract: Two Ca2+-requiring proteinases have been purified from rabbit liver cytosol and shown to be present in isolated hepatocytes. They differ in relative molecular mass, with the major and minor forms, Mr = 150,000 and Mr = 200,000, accounting for 75 and 18% of the total cytosolic neutral proteinase activity, respectively. Both are recovered as inactive proenzymes that can be converted to the active, low-Ca2+-requiring proteinases by incubation with Ca2+ and substrate [S. Pontremoli, E. Melloni, F. Salamino, B. Sparatore, M. Michetti, and B. L. Horecker (1984) Proc. Natl. Acad. Sci. USA 81, 53-56. Each proenzyme is composed of two subunits, with molecular masses of 80 and 100 kDa, respectively. Activation of the proenzymes was found to correlate with their dissociation into subunits. The optimum pH for conversion of the proenzymes to the active proteinases in the presence of 5 mM Ca2+ and 2 mg/ml of denatured globin was approximately 7.5, and the same pH optimum was observed for the digestion of denatured globin by the activated proteinases. Following activation, each proteinase was observed to undergo autolytic inactivation at rates that were dependent on the concentration of both Ca2+ and the digestible substrate. A model is proposed for the activation of the proenzymes and the subsequent inactivation of the active proteinases.
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002154:Calpain; D002469:Cell Separation / Q000379:methods; D003600:Cytosol / Q000201:enzymology; D010450:Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D011817:Rabbits
chemical_list: D004792:Enzyme Precursors; D010450:Endopeptidases; D002154:Calpain; D002118:Calcium