Iron-induced changes in rat liver isoferritins.
The effects of single and of multiple iron injection on the distribution of isoferritins was studied in rat liver with the aid of 14C-labelling either after or before iron treatment. Several effects of iron can be seen. Analysis of protein and labelling patterns show that it not only produces a disproportionate increase in the more-basic isoferritins, but may, in sufficient dose, actually lead to a decrease in the more-acidic isoferritins. Use of iron injection after radioactivity shows that it must give rise to post-assembly changes causing acidic isoferritins to become more basic. With a moderate iron dose this change is relatively slow, taking several hours, and seems to occur in addition to a differential stimulation of the synthesis of the more-basic isoferritins. With higher iron dosage the post-assembly changes may be so rapid that it is difficult to distinguish them from a switch in the pattern of synthesis.
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002250:Carbon Radioisotopes; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D007525:Isoelectric Focusing; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017693:Sodium Bicarbonate
D001639:Bicarbonates; D002250:Carbon Radioisotopes; D011506:Proteins; D017693:Sodium Bicarbonate; D005293:Ferritins; D007501:Iron