Contrasting effects of cytochrome C on the radiosensitivity of single-stranded phi X174 DNA in the presence of misonidazole or phenol under anoxia.
The effect of misonidazole and cytochrome c on biologically active DNA was studied. In a solution of single-stranded phi X174 DNA the presence of both sensitizer and cytochrome c is needed to evoke radiosensitization. However, sensitizer or cytochrome c alone protects the DNA. Cytochrome c offers even an extra protection. The sensitization is dependent on the concentration of misonidazole. This finding opens up the possibility of studying sensitizers in a relatively simple system.
D010584:Bacteriophage phi X 174; D003037:Cobalt Radioisotopes; D003574:Cytochrome c Group / Q000494:pharmacology*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D008920:Misonidazole / Q000494:pharmacology*; D009593:Nitroimidazoles / Q000494:pharmacology*; D010100:Oxygen / Q000502:physiology*; D019800:Phenol; D010636:Phenols / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D003037:Cobalt Radioisotopes; D003574:Cytochrome c Group; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009593:Nitroimidazoles; D010636:Phenols; D011837:Radiation-Protective Agents; D011838:Radiation-Sensitizing Agents; D019800:Phenol; D008920:Misonidazole; D010100:Oxygen