Protein export in Escherichia coli requires a soluble activity.
By using a reconstituted cell-free system we have demonstrated the existence of a soluble activity that is required for the export of proteins in Escherichia coli. This export factor sediments at about 12 S. It has been partially purified by passage through an omega-NH2-butylagarose column and by salt elution off a DEAE matrix. The export factor does not contain 6S RNA.
D001426:Bacterial Proteins / Q000378:metabolism*; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; D012329:RNA, Bacterial