[Regulation of purified NAD-isocitrate dehydrogenase from the pig heart by calcium ions and cAMP-dependent protein kinase].
Different cAMP-dependent protein kinases do not phosphorylate homogeneous NAD-isocitrate dehydrogenase (ICDH) and do not change its activity. Ca2+ (3 X 10(-7) = 10(-3) M) activates the enzyme throughout the process of purification including homogeneous enzyme by decreasing the KM for isocitrate. The calmodulin inhibitor trifluoperazine does not change ICDH activation.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002395:Catecholamines / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D013552:Swine
D002395:Catecholamines; D009243:NAD; D000242:Cyclic AMP; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D011494:Protein Kinases; D002118:Calcium
Reguliatsiia ochishchennoĭ NAD-izotsitratdegidrogenazy serdtsa svin'i ionami kal'tsiia i tsAMF-zavisimoĭ proteinkinazoĭ.