Separation and subfractionation of small numbers of cells (approximately 10(6)) by countercurrent distribution in dextran-poly(ethylene glycol) aqueous-phase systems.
Separation and subfractionation of cells on the basis of subtle differences in surface properties by partitioning in dextran-poly(ethylene glycol) aqueous phase systems is an established method. We report here that the incorporation of fetal bovine serum into such systems permits countercurrent distribution of small quantities of cells (approximately 10(6]. In the absence of serum such small quantities of cells are lost (probably by adherence) and cannot be recovered after countercurrent distribution.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D003377:Countercurrent Distribution; D003911:Dextrans; D004912:Erythrocytes / Q000166:cytology; D005312:Fetal Blood; D066298:In Vitro Techniques; D008297:Male; D011092:Polyethylene Glycols; D011817:Rabbits; D051381:Rats; D012756:Sheep
D003911:Dextrans; D011092:Polyethylene Glycols