Copper(I) transfer into apo-stellacyanin using copper(I)-thiourea as a copper-thionein model.
The direct incorporation of Cu(I) from [Cu(I)(thiourea)3]Cl, a structural analogue of Cu-thionein, into apo-stellacyanin, was successful both aerobically and anaerobically. A characteristic c.d. band of Cu(I)-stellacyanin at 270 nm (0 = -12.5 X 10(3) degrees X cm2 X dmol-1) was seen. On oxidation with hexacyanoferrate(III) or by air, the correct Cu(II) binding into the active centre of this 'Type 1' Cu-protein was deduced from chiroptical measurements which were supported by e.p.r. data. Thus Cu-thiourea turned out to be an excellent Cu(I)-donor in aqueous systems for the complete reconstitution of mononuclear Blue copper proteins.
D001059:Apoproteins; D002942:Circular Dichroism; D003300:Copper; D004578:Electron Spin Resonance Spectroscopy; D008667:Metalloproteins; D008668:Metallothionein; D008956:Models, Chemical; D009942:Organometallic Compounds; D010940:Plant Proteins; D013890:Thiourea / Q000031:analogs & derivatives*
D001059:Apoproteins; D008667:Metalloproteins; D009942:Organometallic Compounds; D010940:Plant Proteins; C007121:copper thionein; C003343:stellacyanin protein, plant; C042723:copper(I)-tris(thiourea); D003300:Copper; D008668:Metallothionein; D013890:Thiourea