Double-stranded cDNAs of hop stunt viroid are infectious.
Restriction fragments composed of only hop stunt viroid (HSV) cDNA were prepared from recombinant clone pHS-P4P, which carries four tandemly repeated HSV cDNAs, and inoculated into cucumber cotyledons. The results showed that double-stranded cDNAs consisting of 1 to 3 units of HSV sequences were infectious. In cucumber plants inoculated with these double-stranded cDNAs, infectious RNA molecules indistinguishable from authentic HSV were propagated.
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D010935:Plant Diseases; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity; D014772:Viroids / Q000235:genetics* / Q000472:pathogenicity
D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes