title: Nature and origin of the RNA associated with simian virus 40 large tumor antigen.
abstract: Simian virus 40 (SV40) large tumor (T) antigen isolated from mammalian cells undergoing lytic or transforming infection is associated with small RNA fragments ("T-antigen RNA") that are protected from nuclease digestion. The rather high complexity of the ribonuclease T1 fingerprints of T-antigen RNA suggested that it is mainly derived from cellular heterogeneous nuclear RNAs. In the present study, 5'-32P-labeled T-antigen RNA was hybridized to monkey, mouse, and human Alu and SV40 DNA, and the nucleotide sequence of 37 T1 oligonucleotides was determined. The results suggest that the bulk of T-antigen RNA is derived from noncoding, double-stranded, ordered regions of cellular heterogeneous nuclear RNAs that exhibit sequence homologies with interspersed repetitive elements of the cellular genome. The possible biological implications of these results are discussed.
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D006801:Humans; D007668:Kidney; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA; D004262:DNA Restriction Enzymes