Indomethacin enhances histamine-stimulated acid production in rabbit isolated fundic glands.
The effects of 10(-4)M indomethacin on tissue prostaglandin E2 levels, cyclic AMP concentrations, and acid secretion as measured by the intraglandular accumulation of [14C]aminopyrine were evaluated in unstimulated and histamine-stimulated isolated rabbit fundic glands. Indomethacin (10(-4)M) reduced endogenous prostaglandin E2 content by 52% in unstimulated glands. Indomethacin tripled histamine (10(-6)-10(-4)M-stimulated aminopyrine uptake while not affecting aminopyrine accumulation in unstimulated glands. Indomethacin did not affect cyclic AMP levels in either the unstimulated or stimulated condition.
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D015232:Dinoprostone; D005744:Gastric Acid / Q000378:metabolism*; D005748:Gastric Fundus / Q000187:drug effects* / Q000502:physiology; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D011458:Prostaglandins E / Q000378:metabolism; D011817:Rabbits
D011458:Prostaglandins E; D000632:Aminopyrine; D006632:Histamine; D000242:Cyclic AMP; D015232:Dinoprostone; D007213:Indomethacin