Characterization of [3H]phenobarbital binding to rat brain membranes.
The binding of [3H]phenobarbital to rat brain membranes was studied in order to determine its characteristics and specificity. The binding reaction was rapid and occurred at sites of low affinity. (Kd = 700 microM) and very high density (Bmax = 2.7 nmol/mg protein). It was unaffected by temperature changes from 0 degrees C to 95 degrees C and was maximal at pH 5. Detergents in low concentrations markedly decreased the binding, apparently without solubilizing the binding sites. It is concluded that the binding of [3H]phenobarbital is a rather non-specific interaction with the plasma membrane.
D000818:Animals; D001463:Barbiturates / Q000378:metabolism; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D010634:Phenobarbital / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism; D011983:Receptors, Purinergic; D013574:Synaptosomes / Q000378:metabolism
D001463:Barbiturates; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D011983:Receptors, Purinergic; D010634:Phenobarbital