In vitro studies on feline panleucopaenia virus. Standardisation of haemagglutination-inhibition test for feline panleucopaenia virus antibody.
Standardised procedure for obtaining reproducible haemagglutination-inhibition results for FPV antibody which correlate with serum-neutralization titres was described. Optimal conditions were found to be Alsevers anticoagulant, PBS/0.05% BSA (pH 6.8) as buffer, especially washed round bottom microplates, determination of maximally sensitive porcine erythrocytes, use of reproducible erythrocyte concentrations, inactivation of serum samples at 56 degrees C for 30 min and serum treatment with koalin pH 9.0. The concentration of erythrocyte used for estimation of haemagglutination units in H1 test should not differ from that used as indicator in the test. Predilution of serum beyond 1:4 associated with false results. Reproducible method for removing natural agglutinins in serum by adsorption with erythrocytes was described.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002415:Cats / Q000382:microbiology*; D028301:Feline Panleukopenia Virus / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests / Q000379:methods / Q000662:veterinary*; D010321:Parvoviridae / Q000276:immunology*; D013696:Temperature
D000914:Antibodies, Viral