[Isolation of virus-specific double-stranded RNA from cells transformed with Rous sarcoma virus].
Several methods were used for isolation of double-stranded (ds) RNA from the cytoplasm of Rous sarcoma virus-transformed chick embryo cells. The dsRNA was shown to have a high melting temperature (82.5 degrees C) in 0.16 M phosphate buffer (pH 6.8), which shifted to more than 90 degrees C after RNase treatment. The size of a single strand was approximately 1300-1600 nucleotides and RNase-resistant fragments were 50-250 nucleotides long. Double-stranded RNA formed hybrids with the labeled genomic RSV RNA RNA so that the major subpopulation of the dsRNA hybridized to 6-10% of RSV RNA and the minor subpopulation -- to 90-94% of RSV RNA. It was suggested that this large subpopulation of dsRNA was abundant in sequences homologous to proviral end fragments as judged by Southern procedure. The data are discussed by considering the analogy between retroviral proviruses and mobile genetic elements.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D066298:In Vitro Techniques; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010766:Phosphorylation; D014176:Protein Biosynthesis; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D004279:DNA, Viral; D012330:RNA, Double-Stranded; D004262:DNA Restriction Enzymes
Vydelenie virusspetsificheskoĭ dvuspiral'noĭ RNK iz kletok, transformirovannykh virusom sarkomy Rausa.