Comparative effects of insulin and insulin-like growth factors on DNA synthesis and differentiation of porcine granulosa cells.
The effects of insulin-like growth factors (IGF-I and II) were compared with those of insulin in porcine granulosa cells in vitro with regard to stimulation of thymidine incorporation, facilitation of FSH-induced progesterone secretion, and displacement of [125I] IGF-I from specific receptors. In all three assays, the order of potency was IGF-I from specific receptors. In all three assays, the order of potency was IGF-I greater than IGF-II greater than insulin. However, the difference in potency was marginally significant in the mitogenic assay. These data are consistent with the presence of specific IGF-I receptors on these cells and involvement of these binding sites in regulation of granulosa cell replication and differentiation.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism; D017451:Receptors, Somatomedin; D013002:Somatomedins / Q000378:metabolism / Q000494:pharmacology*; D013552:Swine
D007328:Insulin; D010455:Peptides; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins; D011374:Progesterone