[Functional, histological, ultrastructural and biochemical study of rat kidney treated with fosfomycin and gentamycin, administered separately or together].
Wistar rats received by intraperitoneal injection for 8 days different doses of Fosfomycin, a new antibiotic. No renal pathological changes are seen under light microscopy after these treatments. Ultrastructural study reveal an intact cellular structure of the proximal tubule. Fosfomycin induces no change in the lysosomal structural latency and enzymatic study show no change in the activities of our hydrolases (alanine-aminopeptidase, alpha-galactosidase, N-acetyl-beta-D-glucosaminidase and sphingomyelinase), after treatment by 100 and 500 mg/kg Fosfomycin. At 1000 mg/kg, Fosfomycin induces an inhibition in enzymatic activities of the four hydrolases. Fosfomycin does not modify the activity of Gentamicin on the proximal tubular cell and induces neither protection nor potentialization of its nephrotoxic effect.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018826:CD13 Antigens; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D005578:Fosfomycin / Q000008:administration & dosage / Q000494:pharmacology*; D005839:Gentamicins / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D005578:Fosfomycin; D013108:Sphingomyelin Phosphodiesterase; D000519:alpha-Galactosidase; D000118:Acetylglucosaminidase; D000626:Aminopeptidases; D018826:CD13 Antigens
Etude fonctionnelle, histologique, ultrastructurale et biochimique du rein de rat traité par la fosfomycine et la gentamycine, administrées seules ou en association.