Characterization of nude mouse skin fibroblast cell lines transformed by combined action of SV40 and 3-methylcholanthrene.
Properties of transformed cell lines derived from secondary cultures of newborn NMRI nu/nu (nude) mouse skin fibroblasts by the sequential exposure of 3-methylcholanthrene and a DNA virus, SV40, were studied. Such transformants were compared to cells transformed by 3-methylcholanthrene or SV40 alone for the tumourigenicity, T-antigen expression, different in vitro growth characteristics and natural killer (NK) cell sensitivity. Despite a considerable variation within a group, the cell lines transformed by the combination treatment as a group were more tumourigenic than cell lines of other groups. In addition, the cell lines transformed by the combination treatment showed increased amounts of T-antigen as compared to cell lines transformed by SV40 alone. They also had, on an average, shorter population doubling time, higher cell saturation density, and a higher amount of DNA per cell than cell lines transformed by SV40 alone. Combination treatment cell lines (5 out of 8) grew in soft agar, whereas cell lines transformed by SV40 or 3-methylcholanthrene alone did not. In conclusion, the cell lines transformed by the combination treatment of 3-methylcholanthrene and SV40 had properties related to malignancy more often than cell lines transformed by SV40 or 3-methyl cholanthrene alone.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008748:Methylcholanthrene; D051379:Mice; D008819:Mice, Nude; D013539:Simian virus 40 / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D008748:Methylcholanthrene; D004247:DNA