Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase.
In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition.
D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes