In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM.
Following infection of RN2 rat Schwannoma cells with unfiltered JHMV inocula, a cell line with an altered phenotype evolved, which was shown to be a somatic cell hybrid of RN2 and mouse L-2 cells. This cell line, EJ, persistently yields JHMV at titres greater than 10(6) pfu/ml and does not show the suppression of virus production at 39.5 degrees C that is characteristic of a persistently infected RN2 line. Intracellular viral nucleocapsids are demonstrated. Cloning of EJ hybrids yields cell lines that show a variety of responses to infection by JHMV or MHV3.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D005347:Fibroblasts / Q000382:microbiology; D006822:Hybrid Cells; D051379:Mice; D008854:Microscopy, Electron; D006517:Murine hepatitis virus / Q000254:growth & development* / Q000302:isolation & purification; D009442:Neurilemmoma / Q000382:microbiology*; D051381:Rats; D014779:Virus Replication
D000956:Antigens, Viral