Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.
The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were transferred onto a plasmid vector by recombination in vivo. Restriction enzyme analysis of the resultant plasmids suggests that some of these mutants arose from either an insertion or a deletion of DNA occurring within the region of DNA that contains the tyrP promoter.
D000818:Animals; D001693:Biological Transport, Active; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D006168:Guinea Pigs; D007700:Kinetics; D007763:Lac Operon; D009154:Mutation; D009876:Operon; D010957:Plasmids; D013045:Species Specificity; D014443:Tyrosine / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D014443:Tyrosine; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase