Intracellular localization of palmitoyl-CoA hydrolase and palmitoyl-CoA synthetase in human blood platelets and liver.
The intracellular localization studies of human blood platelets by sucrose gradient and differential centrifugation, showed that palmitoyl-CoA hydrolase was mainly localized in the cytosol fraction. However, a localization also in the mitochondrial fraction seems possible as disruption of platelets by the French press and nitrogen decompression techniques resulted in mitochondrial damage. In human liver the palmitoyl-CoA hydrolase was localized in the mitochondrial and microsomal fractions. Palmitoyl-CoA synthetase was localized in the mitochondrial and microsomal fractions in both platelets and liver. The possible physiological implications of these differences, and the finding of a very high ratio of palmitoyl-CoA hydrolase/palmitoyl-CoA synthetase in platelets compared with liver, are discussed.
D001792:Blood Platelets / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D003066:Coenzyme A Ligases / Q000032:analysis*; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D010171:Palmitoyl Coenzyme A / Q000097:blood; D010170:Palmitoyl-CoA Hydrolase / Q000032:analysis* / Q000097:blood; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D013869:Thiolester Hydrolases / Q000032:analysis*; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010171:Palmitoyl Coenzyme A; D013869:Thiolester Hydrolases; D010170:Palmitoyl-CoA Hydrolase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase