title: Response of sheep to challenge infection with Fasciola hepatica.
abstract: Sheep were infected with 100 metacercariae of Fasciola hepatica and reinfected 16 weeks later with a further 100 metacercariae. Serum samples were taken weekly for 36 weeks after primary infection. Serum was assayed for the presence of the enzymes glutamate dehydrogenase (GLDH) and gamma-glutamyl transferase (gamma-GT), as indicators of liver and bile duct damage respectively, and for levels of precipitating antibody. Antibody and GLDH levels rose following the primary infection but fell after patency had been reached . A peak in gamma-GT activity was associated with the onset of patency. After the challenge infection levels of both enzymes rose substantially and there were persistent fluctuations in activity. Antibody levels did not rise markedly following challenge but fluctuated at low levels until autopsy, 20 weeks after challenge. There was no resistance to challenge judged by worm recoveries at autopsy. It is suggested that the presence of adult flukes in the bile ducts suppresses the antibody response to challenge infection. Tissue damage, which is shown by fluctuations in GLDH and gamma-GT levels after adult flukes have become established in the bile ducts, is considered to be due to the feeding activity of adult flukes and the deposition of immune complexes in the liver parenchyma.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D005210:Fasciola hepatica / Q000276:immunology; D005211:Fascioliasis / Q000201:enzymology / Q000276:immunology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005969:Glutamate Dehydrogenase / Q000097:blood; D051381:Rats; D012756:Sheep; D012757:Sheep Diseases / Q000201:enzymology / Q000276:immunology*; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000936:Antigen-Antibody Complex; D005969:Glutamate Dehydrogenase; D005723:gamma-Glutamyltransferase