Changes in cytosolic free Ca2+ in isolated parietal cells. Differential effects of secretagogues.
The relationship of free cytosolic Ca2+ to secretagogue-dependent activation of acid secretion by the mammalian parietal cell was studied using quin 2 as an intracellular Ca2+ probe. The resting [Ca2+]in of isolated dog parietal cells was found to be 134 +/- 11 nM. Carbachol produced a steady-state increase of [Ca2+]in and its effect was blocked by atropine and Ca2+ -channel blocking agents. Gastrin transiently elevated [Ca2+]in and this was not affected by Ca2+ -channel blocking agents. Neither histamine nor dbcAMP changed resting [Ca2+]in in rabbit parietal cells.
D000634:Aminoquinolines / Q000378:metabolism; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D004285:Dogs; D005456:Fluorescent Dyes; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
D000634:Aminoquinolines; D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D005755:Gastrins; D003994:Bucladesine; D001285:Atropine; D006632:Histamine; D002217:Carbachol; C035739:Quin2; D002118:Calcium