Comparison between luminol- and lucigenindependent chemiluminescence of polymorphonuclear leukocytes.
Phorbolmyristate acetate (PMA) induced both a luminol- and a lucigenindependent chemiluminescence response in normal polymorphonuclear leukocytes (PMNL). PMNL isolated from a patient with a myeloperoxidase (MPO)-deficiency were found to produce almost no luminol-dependent chemiluminescence. The lucigenin-dependent chemiluminescence response was, however, found not to differ between normal and MPO-deficient cells. From these results it was concluded that the luminol- and the lucigenin-dependent chemiluminescence differed in the dependence of MPO. Results obtained with cell-free MPO-H2O2- and superoxide anion producing systems further supported this conclusion.
D000166:Acridines / Q000494:pharmacology*; D002474:Cell-Free System / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009195:Peroxidase / Q000172:deficiency; D011724:Pyridazines / Q000494:pharmacology*
D000166:Acridines; D011724:Pyridazines; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D009195:Peroxidase