title: Activity of DNase associated with replication of Epstein-Barr virus in NPC-204 cells.
abstract: A deoxyribonuclease derived from Epstein-Barr virus (EBV)-producing lymphoblastoid cell line (NPC-204 cells) treated with IUdR was purified by DEAE cellulose, phospho-cellulose, and DNA-cellulose columns with 45% recovery. The activity of the DNase was neutralized by serum of patient with nasopharyngeal carcinoma (NPC). The DNase had a strong requirement for divalent cations and an alkaline pH optimum. Its activity was inhibited by highly ionic strength and polyamines.
mesh_terms: D002460:Cell Line; D003851:Deoxyribonucleases / Q000032:analysis* / Q000037:antagonists & inhibitors; D004790:Enzyme Induction; D004854:Herpesvirus 4, Human / Q000201:enzymology* / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007065:Idoxuridine / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009303:Nasopharyngeal Neoplasms / Q000201:enzymology*; D014779:Virus Replication
chemical_list: D008345:Manganese; D003851:Deoxyribonucleases; D007065:Idoxuridine