title: Erythromycin and spiramycin resistance mutations of yeast mitochondria: nature of the rib2 locus in the large ribosomal RNA gene.
abstract: Two linked genetic loci, rib 2 and rib 3, of yeast mitochondrial genome are the sites of mutations that confer resistance to erythromycin and/or spiramycin. We have examined two mutations at the rib 2 locus. Mutation ER354 was found at the nucleotide position 3993 of the large ribosomal RNA gene; it corresponded to a C to G transversion leading to a double resistance to erythromycin and spiramycin. Mutation SR551 was found also at the same position, but the C was replaced by a T, conferring resistance to spiramycin only. Rib 2 and rib 3 are 836 base pairs apart on the gene sequence, but are very close to each other in the secondary structure of ribosomal RNA.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal / Q000235:genetics*; D004351:Drug Resistance; D004917:Erythromycin / Q000494:pharmacology*; D005800:Genes, Fungal / Q000187:drug effects*; D008040:Genetic Linkage; D007933:Leucomycins / Q000494:pharmacology*; D009154:Mutation; D012335:RNA, Ribosomal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D007933:Leucomycins; D012335:RNA, Ribosomal; D004917:Erythromycin; D004262:DNA Restriction Enzymes