Efficient production of hepatitis B surface antigen using a bovine papilloma virus-metallothionein vector.
We have developed a highly efficient system for producing hepatitis B virus surface antigen in cultured mammalian cells. This system utilizes a recombinant bovine papilloma virus in which the hepatitis surface antigen coding sequences are inserted into the 5' untranslated region of the mouse metallothionein-I gene. Mouse fibroblasts stably transformed with this molecule produce surface antigen at levels as high as 10 mg/L/24 h and can be maintained in continuous culture for up to 85 days.
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000302:isolation & purification; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D014162:Transfection
D006514:Hepatitis B Surface Antigens; D008668:Metallothionein; D004262:DNA Restriction Enzymes