[Effect of monosaccharides on net ion fluxes across the sperm membrane. Contribution to capacitation].
Monosaccharide supply, monosaccharide fluxes and ion fluxes play an important role in the process of capacitation of spermatozoa. Therefore we investigated the influence of D-glucose and D-fructose on the net -fluxes of Na+ and Rb+. The Na+ fluxes were very low and showed no alterations, when glucose or fructose were present in the incubation medium. On the other hand the net-Rb+-fluxes, which can be regarded as representative for the net-K+-fluxes, were energy dependent and ouabain-sensitive. Moreover D-glucose and D-fructose increased significantly the affinity of Rb+ towards its transport system. The specifity of these interactions is discussed.
D005632:Fructose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D011188:Potassium / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013075:Sperm Capacitation / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
D007473:Ion Channels; D005632:Fructose; D012964:Sodium; D005947:Glucose; D012413:Rubidium; D011188:Potassium
Der Einfluss von Monosacchariden auf die Netto-Ionen-Fluxe über die Spermienmembran. Beitrag zur Kapazitation.