Binding of [3H]cimetidine to rat brain tissue.
Binding of [3H]cimetidine to rat brain tissue was investigated, and a saturable binding with dissociation constant 0.22 +/- 0.05 microM found. This binding is inhibited by a range of imidazole-derived histamine H2-receptor antagonists, but not by a number of non-imidazole H2-receptor antagonists. It is concluded that the [3H]cimetidine binding site in rat brain tissue that is labelled in these experiments is not the histamine H2-receptor.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002927:Cimetidine / Q000378:metabolism*; D006146:Guanidines / Q000378:metabolism*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D051381:Rats; D011970:Receptors, Histamine H2 / Q000378:metabolism
D006146:Guanidines; D006635:Histamine H2 Antagonists; D011970:Receptors, Histamine H2; D002927:Cimetidine