Antigen requirements and specificity of a microplate enzyme-lined immunosorbent assay (ELISA) for Marek's disease viral antibodies in chicken, mouse and rabbit sera.
A rapid indirect enzyme linked immunosorbent assay for the detection of antibodies against Marek's disease virus in mouse, chicken and rabbit-sera has been established. Optimal sensitivity was obtained by using 10 micrograms/ml of partially purified viral proteins. Various experimental procedures performed to eliminate nonspecific reactions are described. Best results were obtained by the introduction of NP 40 at a concentration of 0.1% in sera. With mouse sera, the pretreatment of plaques with poly-L-lysine before antigen fixation substantially reduced the occurrence of nonspecific reactions.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens / Q000276:immunology; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D008380:Marek Disease / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011200:Poultry; D011201:Poultry Diseases / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012376:Rodent Diseases / Q000276:immunology
D000914:Antibodies, Viral; D007106:Immune Sera