title: Transcriptional control of polarity in Escherichia coli by cAMP.
abstract: In Escherichia coli, 3'5'-adenosine cyclic monophosphate (cAMP) and its receptor protein (CAP) are known to be involved in the control of transcription initiation of catabolic operons. In previous papers we have shown that the cAMP-CAP complex is also involved as a modulator of polarity in polycistronic transcription units. Furthermore we showed that there exists a functional relationship between this complex and the transcription termination protein, Rho. In this work, we measured mRNA synthesis corresponding to the promoter proximal and distal parts of the lac and gal operons by DNA-RNA hybridization. We show that in these operons the main polarity effect is essentially transcriptional and the cAMP-CAP complex decreases polarity by interfering with premature transcription termination.
mesh_terms: D001483:Base Sequence; D000242:Cyclic AMP / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D007763:Lac Operon; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D012234:Rho Factor / Q000378:metabolism; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D011953:Receptors, Cyclic AMP; D012234:Rho Factor; D000242:Cyclic AMP; D004262:DNA Restriction Enzymes