The effect of cycloheximide on nuclear uH2A content.
The effect of intraperitonal cycloheximide administration on acid-soluble rat liver chromatin proteins has been investigated by electrophoresis in acetic acid-urea polyacrylamide gels. A nonhistone protein, which migrates between oxidized histone H3 and histone H1 has been found to be increased in amount following cycloheximide treatment. This protein seems to be identical with semihistone protein H24 (uH2A). A possible relationship of uH2A to the inhibition of rRNA synthesis is discussed.
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D013853:Thioacetamide / Q000494:pharmacology; D014452:Ubiquitins / Q000378:metabolism*
D002843:Chromatin; D006609:High Mobility Group Proteins; D006657:Histones; D014452:Ubiquitins; C021645:chromatin conjugate protein A24; D013853:Thioacetamide; D003513:Cycloheximide