Evidence for involvement of pyrH+ of an Escherichia coli K-12 F-prime factor in inhibiting construction of hybrid merodiploids with Salmonella typhimurium.
Transconjugants were not recovered in matings between Salmonella typhimurium and Escherichia coli K-12 strains carrying the chromosomal region between leu and argF on an F-prime factor, even when a restriction-deficient recipient was used. A mutant F-prime factor compatible for transfer to S. typhimurium was constructed by transposon mutagenesis and characterized as being deficient in directing the synthesis of UMP kinase (encoded by pyrH). Other compatible F-prime factors were readily constructed by employing a procedure designed to select for strains carrying F-prime factors harboring pyrH mutations.
D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005144:F Factor; D005798:Genes, Bacterial; D009154:Mutation; D009703:Nucleoside-Phosphate Kinase / Q000235:genetics; D014176:Protein Biosynthesis; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; C023276:uridine monophosphate kinase; D009703:Nucleoside-Phosphate Kinase