High-performance liquid chromatographic determination of free myxin and its reduction product as impurities in cuprimyxin-containing creams.
A stability-indicating high-performance liquid chromatographic method was developed that can detect and quantitate low levels of the two most likely breakdown products of cuprimyxin. These degradation compounds, free myxin and its reduction product, can be determined in topical cream preparations in which cuprimyxin is formulated at the 0.5% (w/w) level. The method requires a simple two-step extraction, the addition of an internal standard, and chromatography on an amine-bonded column.
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000032:analysis*; D004340:Drug Contamination; D009824:Ointments; D010619:Phenazines / Q000032:analysis
D000900:Anti-Bacterial Agents; D009824:Ointments; D010619:Phenazines; C005247:myxin; D003300:Copper; C000506:cuprimyxin