Induction of tyrosine aminotransferase by pyridoxine in Drosophila hydei.
Salivary glands incubated in various concentrations of pyridoxine (Vitamin B6) show increasing tyrosine aminotransferase (TAT) activity at concentrations up to 10(-5) M and then decreasing activity up to 10(-2) M but in all cases the activity is greater than that of the controls. This increase in activity is demonstrable for up to 6 h, the longest period tested, and is dependent on the synthesis of new mRNA. A similar increase in TAT activity is observed in salivary glands subjected to heat shock. Antibodies prepared against purified tyrosine aminotransferase precipitate a peptide of the same molecular weight (40 KD) as that induced by pyridoxine.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004330:Drosophila / Q000235:genetics*; D004790:Enzyme Induction; D006358:Hot Temperature; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D011736:Pyridoxine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D012469:Salivary Glands / Q000201:enzymology*; D013997:Time Factors; D014158:Transcription, Genetic; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D012333:RNA, Messenger; D014444:Tyrosine Transaminase; D011736:Pyridoxine