Functional size of the human asialoglycoprotein receptor as determined by radiation inactivation.
The radiation target size of the functional asialoglycoprotein receptor (ASGP-R) in purified plasma membranes was determined for human liver and the human hepatoma cell line, Hep G2, by evaluation of the binding of 125I-asialoorosomucoid. Identical inactivation curves were observed for ASGP-R from normal human liver and post-mortem human liver and exhibited a functional unit of 70,000 daltons. The human hepatoma Hep G2 ASGP-R, in contrast, showed a target size of 140,000 daltons. These results suggest that the functional ligand-binding unit of the human ASGP-R is a multimer of the approximately 34,000-dalton unglycosylated receptor polypeptide (Schwartz, A. L., and Rup, D. (1983) J. Biol. Chem. 258, 11249-11255).
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism; D008970:Molecular Weight; D009961:Orosomucoid / Q000031:analogs & derivatives / Q000378:metabolism; D011971:Receptors, Immunologic / Q000032:analysis*
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D009961:Orosomucoid; D011971:Receptors, Immunologic; C017030:asialoorosomucoid