title: Experimental infections in Venezuelan lizards by Trypanosoma cruzi.
abstract: Virulent trypomastigotes of the Y strain of Trypanosoma cruzi were administered to Tropidurus hispidus, Ameiva ameiva, Cnemidophorus lemniscatus, Polychrus marmoratus, and Phyllodactylus ventralis (Sauria). Intraperitoneal and subcutaneous inoculations of lizards with mouse blood or with feces of infected Rhodnius prolixus (Reduviidae, Triatominae), as well as forced ingestion of triturated Rhodnius, produced no parasitaemias detectable either directly or by xenodiagnosis, while control mice became parasitized. Pretreatment with the immunosuppressive drug Fluocinolone acetonide led to establishing patent infections in inoculated lizards. Cryptic infections were established by inoculation of 1 X 10(6) parasites from Davis' medium, or by 95 X 10(3) parasites from lizard tissue culture. Parasites were not seen in tissues. Mice inoculated with blood or tissue homogenates from these lizards became parasitized. Parasites from Davis' medium inoculated into the peritoneal cavity of lizards were capable, to a very low degree, of penetrating the free peritoneal macrophages and changing into amastigotes. The factors possibly responsible for the natural resistance of poikilothermic vertebrates to T. cruzi are discussed.
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007113:Immunity, Innate; D007165:Immunosuppression Therapy; D008116:Lizards / Q000469:parasitology*; D051379:Mice; D014349:Trypanosoma cruzi / Q000472:pathogenicity; D014774:Virulence