title: [Site-specific endonuclease BmeI from Bacillus megaterium 216].
abstract: A site-specific endonuclease BmeI has been isolated from Bacillus megaterium 216 by gel filtration on ultragel AcA-44 with a subsequent chromatography on heparin-sepharose 6B. On the double-stranded DNA the endonuclease recognizes the pentanucleotide sequence (Formula: see text); and hydrolyzes it in the points shown by arrows. At gel filtration the endonuclease is eluted in the volume corresponding to a molecular mass of 60 000.
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004262:DNA Restriction Enzymes / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C042767:endodeoxyribonuclease BmeI; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Saĭt-spetsificheskaia éndonukleaza BmeI iz Bacillus megaterium 216.