Production of steroid hormone and cyclic AMP in hybrids of adrenal and Leydig cells generated by electrofusion.
Electrofusion of rat adrenal and Leydig cells generated hybrids capable of synthesizing simultaneously both testosterone and corticosterone, under stimulation of lutropin or adrenocorticotropin. Evidence was obtained indicating that under such circumstances, heterologous lutropin receptor--adrenal adenylate cyclase complexes were formed.
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002459:Cell Fusion; D003345:Corticosterone / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004560:Electricity; D006822:Hybrid Cells / Q000378:metabolism*; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D051381:Rats; D013739:Testosterone / Q000096:biosynthesis*
D013739:Testosterone; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D000242:Cyclic AMP; D003345:Corticosterone