Enzyme-linked immunosorbent assay for detection of receptors for polymerized human serum albumin on hepatitis B surface antigen particles.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect the polymerized human serum albumin (pHSA) receptor on hepatitis B virus surface antigen (HBsAg) particles. The receptor values on particles from HBeAg-positive patients were significantly higher than those from anti-HBe-positive and HBeAg-, anti-HBe-negative patients. In patients' sera with moderate to high receptor values, there was significant correlation between the values obtained in the ELISA and serum DNA-polymerase activity (P less than 0.005), but not with HBeAg titer. During a 1-year follow-up of 47 HBeAg-positive patients with chronic hepatitis, termination of active viral replication, as evidenced by seroconversion from HBeAg to anti-HBe-positive, was observed in 1 of 26 with high initial receptor values, 2 of 12 with moderately high values and 6 of 9 with low receptor values. The results suggest that the ELISA described, which detects pHSA receptors on HBsAg particles, might be of value as a prognostic test in HBeAg-positive chronic hepatitis type B.
D004259:DNA-Directed DNA Polymerase / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000201:enzymology / Q000503:physiopathology*; D006511:Hepatitis B Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006801:Humans; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology
D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D004259:DNA-Directed DNA Polymerase