Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.
The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.
D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004351:Drug Resistance; D004926:Escherichia coli / Q000235:genetics*; D006921:Hygromycin B / Q000494:pharmacology; D019868:Kanamycin Kinase; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014162:Transfection
D006921:Hygromycin B; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D004262:DNA Restriction Enzymes