Comparison of methods for the isolation and purification of infectious laryngotracheitis virus DNA.
Infectious laryngotracheitis virus (ILTV) DNA was prepared using sodium iodide equilibrium centrifugation, preferential phosphorus-32 labelling and extraction with the detergent Triton X 100. The Triton X 100 procedure was found to be the preferred method for preparing DNA for analytical purposes. A method for ILTV titre enhancement is described.
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004279:DNA, Viral / Q000302:isolation & purification*; D004586:Electrophoresis; D006564:Herpesviridae / Q000235:genetics*; D001352:Herpesvirus 1, Gallid / Q000235:genetics*; D007668:Kidney; D010761:Phosphorus Radioisotopes; D011092:Polyethylene Glycols
D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D011092:Polyethylene Glycols