Sequential conversion of the glutathionyl side chain of slow reacting substance (SRS) to cysteinyl-glycine and cysteine in rat basophilic leukemia cells stimulated with A-23187.
In rat basophilic leukemia (RBL-1) cells stimulated with A-23187, the major slow reacting substance (SRS) species contain glutathione, cysteinyl-glycine, or cysteine in their side chains, corresponding or closely related to leukotrienes LTC4, LTD4, and LTE4, respectively. Evidence is presented that most of the SRS produced during the first few minutes of stimulation by the ionophore has a glutathionyl side chain which is sequentially converted to cysteinyl-glycine and cysteine.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000378:metabolism; D012898:Autacoids / Q000378:metabolism*; D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006168:Guinea Pigs; D007942:Leukemia, Experimental / Q000378:metabolism*; D051381:Rats; D013189:SRS-A; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001095:Arachidonic Acids; D012898:Autacoids; D004151:Dipeptides; D013189:SRS-A; D000001:Calcimycin; C028505:cysteinylglycine; D005723:gamma-Glutamyltransferase; D005978:Glutathione; D003545:Cysteine