title: Repression and derepression of the enzymes of the pyrimidine biosynthetic pathway in Salmonella typhimurium.
abstract: Activities of five enzymes of the pyrimidine biosynthetic pathway and one enzyme involved in arginine synthesis were measured during batch culture of Salmonella typhimurium. Aspartate carbamoyltransferase, dihydroorotase, and the arginine pathway enzyme, ornithine carbamoyltransferase, remained constant during the growth cycle but showed a sharp decrease in activity after entering the stationary phase. Dihydroorotate dehydrogenase, orotate phosphoribosyltransferase and orotidine-5'-monophosphate (OMP) decarboxylase showed peaks of activity corresponding to the mid-point of the exponential phase of growth while remaining comparatively stable in the stationary phase. Derepression studies carried out by starving individual pyrimidine (Pyr-) deletion mutants for uracil showed that the extent of derepression obtained for aspartate carbamoyltransferase, dihydroorotase and dihydroorotate dehydrogenase depended on the location of the pyr gene mutation. Orotate phosphoribosyltransferase and OMP decarboxylase derepression levels were independent of the location of the pyr mutation. Aspartate carbamoyltransferase showed the greatest degree of derepression of the six enzymes studied, with pyrA strains (blocked in the first step of the pathway) showing about twice as much derepression as pyrF strains (blocked in the sixth step of the pathway). A study of the kinetics of repression on derepressed levels of the pyrimidine enzymes produced data that were compatible with dilution of specific activity by cell division when repressive amounts of uracil were added to the derepression medium.
mesh_terms: D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D004080:Dihydroorotase / Q000378:metabolism; D004081:Dihydroorotate Oxidase / Q000378:metabolism; D004794:Enzyme Repression; D007700:Kinetics; D009154:Mutation; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D009962:Orotate Phosphoribosyltransferase / Q000378:metabolism; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000378:metabolism; D011743:Pyrimidines / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000235:genetics; D014498:Uracil / Q000378:metabolism
chemical_list: D011743:Pyrimidines; D014498:Uracil; D004081:Dihydroorotate Oxidase; D001221:Aspartate Carbamoyltransferase; D009954:Ornithine Carbamoyltransferase; D009962:Orotate Phosphoribosyltransferase; D004080:Dihydroorotase; D009964:Orotidine-5'-Phosphate Decarboxylase