Involvement of rat corticotrophin-releasing factor-41-related peptide and vasopressin in adrenocorticotrophin-releasing activity from superfused rat hypothalami in vitro.
Superfused rat hypothalamic pieces stimulated with a high K+ medium released corticotrophin-releasing factor (CRF) as detected in the rat isolated anterior pituitary cell column bioassay. This activity has components related to ovine CRF-41 (oCRF-41) and vasopressin as assessed by the effects of specific antisera on the ACTH-releasing activity of the hypothalamic column effluent. Release of immunoactive vasopressin paralleled that of CRF bioactivity. Immunoactive oCRF-41 could not be detected, as it is likely to be below the sensitivity of present radioimmunoassays.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism / Q000502:physiology*; D001681:Biological Assay; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D003470:Culture Media; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology; D066298:In Vitro Techniques; D010455:Peptides / Q000378:metabolism / Q000502:physiology*; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D007106:Immune Sera; D010455:Peptides; D001127:Arginine Vasopressin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D011188:Potassium