Functional diversity in OKT4 and OKT8 subsets from long-term survivors after HLA-identical marrow grafting.
T and B cells from one short-term and nine long-term patients who had received HLA-identical bone marrow transplants for aplastic anemia or hematologic malignancy were studied for their ability to synthesize immunoglobulin after in vitro stimulation with pokeweed mitogen, Herpes simplex type 1 antigen, tetanus toxoid, or Epstein-Barr virus. Purified T, OKT4, OKT8, or B cells from patients were cocultured with normal T and/or B cells in the presence of the various stimulants. Multiple regulatory defects were observed in long-term patients with and without chronic graft-versus-host disease. The lymphocytes from the long-term healthy chimeras exhibited fewer defects in their ability to regulate in vitro immunoglobulin synthesis than those of patients with chronic graft-versus-host disease. These findings suggest that the presence of chronic graft-versus-host disease delays or impairs the "rematuration" of the immune system after bone marrow grafting and that the rematurational process occurs at the clonal level.
D000911:Antibodies, Monoclonal; D000921:Antibody-Producing Cells / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002472:Cell Transformation, Viral; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation; D011043:Pokeweed Mitogens / Q000494:pharmacology; D018139:Simplexvirus / Q000502:physiology; D013601:T-Lymphocytes / Q000145:classification*; D013745:Tetanus Toxoid / Q000494:pharmacology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D011043:Pokeweed Mitogens; D013745:Tetanus Toxoid