Expression of liver phenotypes in cultured mouse hepatoma cells.
Mouse hepatoma cells were established in vitro as a permanently growing line designated Hepa. The mass population and a subclone were characterized for their karyotype and their retention of liver-specific properties. An examination of 17 hepatic traits revealed that the cell lines secreted several serum proteins. The activities of a number of liver-specific enzymes, however, appeared to be absent in these cells. The identification of differentiated properties of cultured hepatoma cells permits the use of these lines in a variety of studies such as cell hybridization, biochemical analysis of tissue-specific gene products, and the modulation of expression of genes governing differentiated phenotypes. This report presents the analysis of a broad spectrum of characteristics and thereby describes one of the most fully defined hepatoma cell lines of murine origin in the literature.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002460:Cell Line; D002570:Ceruloplasmin / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007621:Karyotyping; D008112:Liver Glycogen / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D011506:Proteins / Q000378:metabolism; D014444:Tyrosine Transaminase / Q000378:metabolism
D001798:Blood Proteins; D007527:Isoenzymes; D008112:Liver Glycogen; D011506:Proteins; D001189:Aryl Hydrocarbon Hydroxylases; D002570:Ceruloplasmin; D014444:Tyrosine Transaminase; D002802:Cholinesterases; D005634:Fructose-Bisphosphate Aldolase