The activities of enzymes of purine metabolism in murine thymus dependent lymphocytes.
Measurement of the activities of purine metabolizing enzymes in murine T cell subpopulations showed that these activities differed markedly among T cells of different levels of functional maturity. The activities of adenosine deaminase and deoxyadenosine phosphorylation were highest in immature, PNA + thymocytes, while the activities of purine nucleoside phosphorylase, ecto-5'-nucleotidase and deoxyguanosine phosphorylation were highest in mature, splenic T cells. These enzymes' activities can be used as biochemical markers for T cell of different degree of maturation.
D015720:5'-Nucleotidase; D000243:Adenosine Deaminase / Q000032:analysis; D000248:Adenosine Kinase / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D003842:Deoxycytidine Kinase / Q000032:analysis; D037102:Lectins / Q000494:pharmacology; D008297:Male; D051379:Mice; D009708:Nucleotidases / Q000032:analysis; D019887:Peanut Agglutinin; D010770:Phosphotransferases / Q000032:analysis; D017853:Phosphotransferases (Alcohol Group Acceptor); D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis; D011687:Purines / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D037102:Lectins; D019887:Peanut Agglutinin; D011687:Purines; D011683:Purine-Nucleoside Phosphorylase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C019165:deoxyguanosine kinase; D000248:Adenosine Kinase; D003842:Deoxycytidine Kinase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase