Identification of a gene function of herpes simplex virus type 1 essential for amplification of simian virus 40 DNA sequences in transformed hamster cells.
Infection with herpes simplex viruses (HSV) lead to a significant increase of the simian virus 40 (SV40) DNA content in the SV40-transformed hamster cell lines CO631 and Elona. Analysis of this gene-amplifying activity revealed (i) that it cosedimented with infectious herpesvirions in sucrose density gradients, (ii) that it was abolished by anti-HSV antibodies or (iii) by antiviral drugs acting on the HSV-induced DNA polymerase; and analysis of temperature-sensitive mutants showed that this DNA polymerase was an essential component of HSV-induced, gene-amplifying activity in SV40-transformed hamster cells.
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004279:DNA, Viral / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D017245:Foscarnet; D005784:Gene Amplification; D005814:Genes, Viral; D009154:Mutation; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics*; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D013696:Temperature; D014415:Tunicamycin / Q000494:pharmacology; D014779:Virus Replication
D004279:DNA, Viral; D014415:Tunicamycin; D017245:Foscarnet; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid; D000212:Acyclovir