[Multiple forms of cyclic nucleotide phosphotransferases in mouse thymocytes].
The presence of cAMP- and cGMP- specific phosphodiesterases in mouse thymocytes has been demonstrated. Sucrose density gradient centrifugation revealed two peaks corresponding to cAMP-phosphodiesterase (3.6S and 6.0S) and one peak corresponding to cGMP-phosphodiesterase (6.6S). Gel filtration demonstrated high molecular forms of the enzymes; according to the rechromatography data, cAMP and cGMP phosphodiesterases are represented by oligomers containing subunits with Ms 45 000 and 160 000 respectively. Incubation of thymocyte lysates for 24-48 hours led to a decrease of the number of high molecular weight forms and an increase in the number of low molecular weight forms paralleled with a rise in V and Km values.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology*
D002147:Calmodulin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases
Issledovanie mnozhestvennykh form fosfodiésterazy tsiklicheskikh nukleotidov v timotsitakh mysheĭ.