Development of a quantitative immunoassay for the cystic fibrosis gene.
An antiserum raised in guineapigs showed positive immunoelectrophoretic reaction with sera from 16 of 17 patients with cystic fibrosis, 8 of 9 obligate heterozygotes, and only 1 of 15 normal subjects. The antiserum appeared to be specific to the cystic-fibrosis protein described by Wilson and colleagues. Precipitin peaks were larger with sera from cystic-fibrosis homozygotes than from heterozygotes, suggesting the possibility of a new quantitative biochemical assay for cystic fibrosis.
D000818:Animals; D000918:Antibody Specificity; D003550:Cystic Fibrosis / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D005796:Genes; D006580:Genetic Carrier Screening; D006168:Guinea Pigs; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007122:Immunoelectrophoresis / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing / Q000379:methods; D009154:Mutation; D011506:Proteins / Q000032:analysis*
D007106:Immune Sera; D007074:Immunoglobulin G; D011506:Proteins