Stimulation of pyruvate dehydrogenase phosphatase activity by polyamines.
Pyruvate dehydrogenase phosphatase requires Mg2+ or Mn2+, and its activity in the presence of Mg2+ is markedly stimulated by Ca2+. At saturating Mg2+ and Ca2+ concentrations, the polyamines spermine, spermidine and putrescine stimulated the activity of pyruvate dehydrogenase phosphatase 1.5- to 3-fold. Spermine was the most active of the polyamines. At a physiological concentration of Mg2+ (1 mM) and saturating Ca2+ concentration, the stimulation by 0.5 mM spermine was 4- to 5-fold, and at 0.3 mM Mg2+, the stimulation was 20- to 30-fold. In the absence of Mg2+ or Ca2+, spermine had no effect. These results suggest that a polybasic factor may be involved in the regulation of pyruvate dehydrogenase phosphatase activity.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D002417:Cattle; D004789:Enzyme Activation; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011073:Polyamines / Q000494:pharmacology*; D054645:Protein Phosphatase 1; D011700:Putrescine / Q000494:pharmacology; D011769:Pyruvate Dehydrogenase (Lipoamide)-Phosphatase / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D002413:Cations, Divalent; D011073:Polyamines; D013096:Spermine; D008345:Manganese; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D011769:Pyruvate Dehydrogenase (Lipoamide)-Phosphatase; D008274:Magnesium; D002118:Calcium; D013095:Spermidine; D011700:Putrescine