Inhibition of cyclic AMP-mediated intestinal hypersecretion by pituitary extracts from rats pretreated with cholera toxin.
Peroral pretreatment with cholera toxin (CT) in rats induced protection against intestinal hypersecretion by CT or prostaglandin E1 (PGE1). Pituitary glands from these CT-pretreated rats were homogenized and injected intravenously or intraluminally into untreated rats. These recipients became resistant to CT- as well as to PGE1-induced hypersecretion; recipients given pituitary extracts from control animals responded normally. Extracts of intestinal mucosa from CT-pretreated, but not from control rats, also inhibited secretion by CT. Ultrafiltration experiments with the pituitary or intestinal extracts indicated that the antisecretory factors had a molecular weight between 10,000 and 50,000.
D000527:Alprostadil; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014020:Tissue Extracts / Q000494:pharmacology
D011458:Prostaglandins E; D014020:Tissue Extracts; D002772:Cholera Toxin; D000242:Cyclic AMP; D000527:Alprostadil