Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence.
Two cDNA clones encoding the predominant form of the asialoglycoprotein receptor from rat liver (the major rat hepatic lectin; RHL-1) were identified by screening a rat liver cDNA library with a mixed oligonucleotide probe 35 nucleotides long. One clone was a nearly full-length copy of the mRNA for RHL-1, while the other was shortened at both ends. The sequences of these clones demonstrate that this transmembrane receptor is not synthesized with an NH2-terminal signal sequence. The only proteolytic processing occurring in the biosynthesis of RHL-1 is the removal of the NH2-terminal initiator methionine residue. Insertion of RHL-1 into the membrane is postulated to occur by the recognition of the internal transmembrane region as a signal sequence.
D000595:Amino Acid Sequence; D000818:Animals; D037263:Asialoglycoprotein Receptor; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009961:Orosomucoid / Q000031:analogs & derivatives; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D037263:Asialoglycoprotein Receptor; D009961:Orosomucoid; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D004247:DNA; D004262:DNA Restriction Enzymes