Neuropeptide Y receptor in the rat brain.
The specific binding of the chloramine-T iodinated neuropeptide Y (125I-NPY) to membranes from rat cerebral cortex was investigated using equilibrium binding and kinetic methods. The equilibrium binding of 125I-NPY at 37 degrees C was characterized by a Kd value of 0.38 nM. The receptor densities in the cerebral cortex, hypothalamus and cerebellum were 0.45 pmol/mg, 0.47 pmol/mg and 0.04 pmol/mg protein respectively. The binding site for 125I-NPY was sensitive to treatment with proteolytic enzymes and thiol reagents. The binding showed a sharp optimum at pH 7-7.7 and was inhibited by increasing concentrations of Mg2+.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009478:Neuropeptide Y; D010084:Oxidation-Reduction / Q000187:drug effects; D011489:Protein Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y; D013347:Subcellular Fractions / Q000378:metabolism; D013439:Sulfhydryl Reagents; D013696:Temperature
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D011956:Receptors, Cell Surface; D017476:Receptors, Neuropeptide Y; D013439:Sulfhydryl Reagents