Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor.
Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investiagtors from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and posses amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D002460:Cell Line / Q000378:metabolism*; D002999:Clone Cells / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D009381:Neoplasms, Radiation-Induced; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D051381:Rats; D013004:Somatostatin / Q000378:metabolism*
D007328:Insulin; D013004:Somatostatin