Formation of gamma-glutamyl-taurine in the rat brain.
After intraventricular administration on 14C- or 3H-labelled taurine, gamma-glutamyl-taurine was detected in the protein free extract of rat brain using a combination of ion exchange chromatography, electrophoretic separation and thin-layer chromatography. Under in vitro conditions gamma-glutamyl-transpeptidase (EC 2.3.2.2.) prepared from rat brain catalyses the formation of gamma-glutamyl-taurine from taurine and glutathione or gamma-glutamyl-p-nitroanilide. The enzymes of the gamma-glutamyl cycle are presumed to be responsible for the in vivo metabolism of this dipeptide.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005973:Glutamine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D010281:Parathyroid Hormone / Q000096:biosynthesis*; D051381:Rats; D013654:Taurine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D014316:Tritium; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002250:Carbon Radioisotopes; D010281:Parathyroid Hormone; D005973:Glutamine; D014316:Tritium; D013654:Taurine; C020903:glutaurine; D005723:gamma-Glutamyltransferase