Effects of DNA-repair-deficient mutants on somatic and germ line mutagenesis in the UZ system in Drosophila melanogaster.
To analyze the underlying mechanisms of the UZ system in Drosophila melanogaster, i.e., the unstable white locus with an IS element included originally described by Rasmuson and Green (1974), the repair deficient mutants mei-9b and mei-4lD5 and the meiotic mutant mei-2l8 were introduced into X-chromosomes containing the UZ system. These strains were submitted to larval feeding of MMS to analyze differences occurring in mutation rate and survival. The mei-9b and mei-4l strains were markedly sensitive to MMS treatment, while mei-2l8 did not affect survival. Only the mutant mei-4l, which is lacking in post-replication repair, affected the somatic mutation rate of the UZ system, decreasing it by 50%. The spontaneous germ line mutation rate of UZ, on the other hand, was increased by introducing mei-4l or mei-9b, possibly as a result of the high frequency of unrepaired spontaneous chromosome breaks that occur in these mutants.
D000818:Animals; D004260:DNA Repair; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005838:Genotype; D007814:Larva / Q000187:drug effects; D008297:Male; D008540:Meiosis; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009152:Mutagenicity Tests; D009154:Mutation; D014178:Translocation, Genetic
D004251:DNA Transposable Elements; D008741:Methyl Methanesulfonate