Myeloperoxidase reduces the opsonizing activity of immunoglobulin G and complement component C3b.
The effect of myeloperoxidase, hydrogen peroxide (H2O2) and a halide (Cl) on the opsonizing molecules in immunoglobulin G (IgG) and complement factor C3b was assayed. At concentrations of the enzyme (1 microgram/ml) that can be found in the extracellular fluid during inflammation, the myeloperoxidase-H2O2-Cl system inhibited the opsonizing effect of IgG and C3b measured as phagocytic uptake and superoxide generation. The effect was related to the enzymatic peroxidative activity of the protein. The presence of albumin (10 mg/ml) reduced the effect of myeloperoxidase with 10-20%. Taurine, which in the presence of myeloperoxidase-H2O2-Cl forms hydrophilic chloramines, and D-penicillamine, which scavenges HOCl, neutralize the inhibitory effect of myeloperoxidase. This suggests that either hypochlorous acid or lipophilic chloramines may exert its effect by oxidizing free sulphydryl groups exposed on the opsonizing ligands. Since the myeloperoxidase-H2O2-halide system also affects chemotactic factors, leukotrienes, proteinases and membrane receptors, the system may in several ways affect the development of the inflammatory response.
D003179:Complement C3b / Q000276:immunology*; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects*; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000276:immunology; D013481:Superoxides / Q000096:biosynthesis
D007074:Immunoglobulin G; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D013481:Superoxides; D003179:Complement C3b; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D010544:Peroxidases; D009195:Peroxidase