Subcellular localization of long-chain alcohol dehydrogenase and aldehyde dehydrogenase in n-alkane-grown Candida tropicalis.
Long-chain alcohol dehydrogenase and long-chain aldehyde dehydrogenase were induced in the cells of Candida tropicalis grown on n-alkanes. Subcellular localization of these dehydrogenases, together with that of acyl-CoA synthetase and glycerol-3-phosphate acyltransferase, was studied in terms of the metabolism of fatty acids derived from n-alkane substrates. Both long-chain alcohol and aldehyde dehydrogenases distributed in the fractions of microsomes, mitochondria and peroxisomes obtained from the alkane-grown cells of C. tropicalis. Acyl-CoA synthetase was also located in these three fractions. Glycerol-3-phosphate acyltransferase was found in microsomes and mitochondria, in contrast to fatty acid beta-oxidation system localized exclusively in peroxisomes. Similar results of the enzyme localization were also obtained with C. lipolytica grown on n-alkanes. These results suggest strongly that microsomal and mitochondrial dehydrogenases provide long-chain fatty acids to be utilized for lipid synthesis, whereas those in peroxisomes supply fatty acids to be degraded via beta-oxidation to yield energy and cell constituents.
D000106:Acetate-CoA Ligase / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism; D002175:Candida / Q000201:enzymology* / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology
D000473:Alkanes; D000429:Alcohol Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; D000445:Aldehyde Oxidoreductases; D000106:Acetate-CoA Ligase