Identification of leukotriene C-1 as a major component of slow-reacting substance from rat mononuclear cells.
Slow-reacting substance (SRS) was produced by rat peritoneal mononuclear cells after stimulation with the ionophore A23187. The SRS consisted of two main components as judged by high-pressure liquid chromatography (HPLC) on Florisil. The larger and more polar component consisted mainly of leukotriene (LT) C-1 as judged by ultraviolet spectroscopy, mass spectrometry (after desulfurization), amino acid analysis, and conversion by soybean lipoxygenase. Comparisons with authentic LTC-1 showed identity on reverse phase HPLC and guinea pig ileum bioassay. The latter methods are known to distinguish between LTC-1 and stereoisomers of LTC-1.
D000596:Amino Acids; D000818:Animals; D001095:Arachidonic Acids / Q000302:isolation & purification*; D012898:Autacoids / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D004537:Eicosanoic Acids / Q000302:isolation & purification; D006168:Guinea Pigs; D008084:Lipoxygenase / Q000494:pharmacology; D010586:Phagocytes; D051381:Rats; D013025:Glycine max
C025347:5-hydroxyarachidic acid; D000596:Amino Acids; D001095:Arachidonic Acids; D012898:Autacoids; D004537:Eicosanoic Acids; D008084:Lipoxygenase