Surface activity of polypeptide toxins isolated from Anemonia sulcata.
Circular dichroism spectra and surface activities are reported for toxins II and III isolated from Anemonia sulcata. Toxin II is highly surface active and as a result possesses a synergistic effect with phospholipase A2. In complete contrast, toxin III lacks detectable surface activity. The presence of sodium dodecyl sulfate (2.5 mg.ml-1) failed to trigger large conformation changes in both toxins II and III. From a comparison of the sequences of toxins II and III it is suggested that the hydrophobic region 17-27 is responsible for the surface activity of toxin II.
D000818:Animals; D002942:Circular Dichroism; D003064:Cnidarian Venoms / Q000302:isolation & purification*; D004357:Drug Synergism; D007700:Kinetics; D008081:Liposomes; D008555:Melitten; D010179:Pancreas / Q000201:enzymology; D010455:Peptides / Q000302:isolation & purification*; D010713:Phosphatidylcholines; D010741:Phospholipases A; D054467:Phospholipases A2; D012615:Sea Anemones / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013499:Surface Properties
D003064:Cnidarian Venoms; D008081:Liposomes; D010455:Peptides; D010713:Phosphatidylcholines; D008555:Melitten; D012967:Sodium Dodecyl Sulfate; C028800:toxin II (Anemonia sulcata); C032104:toxin III (Anemonia sulcata); D010741:Phospholipases A; D054467:Phospholipases A2