Hormonal regulation of transcription of rDNA: use of nucleoside thiotriphosphates to measure initiation in isolated nuclei.
Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Initiation of pre-rRNA was completely resistant to alpha-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal / Q000235:genetics*; D003907:Dexamethasone / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D009694:Nucleic Acid Precursors / Q000235:genetics; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D012322:RNA Precursors; D012335:RNA, Ribosomal / Q000235:genetics; D051381:Rats; D013873:Thionucleotides / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D014316:Tritium
D004275:DNA, Ribosomal; D009694:Nucleic Acid Precursors; D010761:Phosphorus Radioisotopes; D012322:RNA Precursors; D012335:RNA, Ribosomal; D013873:Thionucleotides; D014316:Tritium; C022571:adenosine 5'-O-(3-thiotriphosphate); D016244:Guanosine 5'-O-(3-Thiotriphosphate); D003907:Dexamethasone; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004262:DNA Restriction Enzymes