Regulation of carbohydrate metabolism in cultured mammalian cells: energy provision in a glycolytic mutant.
The metabolism of radioactively labelled D-glucose, L-glutamine, and L-glutamate has been determined in a glycolytic mutant of Chinese-hamster ovary cells, R1.1.7, and in its parent, CHO-K1. The complete oxidation of glucose via the TCA-cycle is negligible in both cell types, but there is significant oxidation of carbon-1. CHO-K1 cells derive most of their energy from glycolysis and are independent of respiration in the short term. R1.1.7 cells are respiration-dependent and are rapidly killed by respiratory inhibitors. Both cell types oxidize L-glutamine and L-glutamate, but oxidation of these substrates does not appear sufficient to satisfy completely the energy requirements of R1.1.7 cells.
D000746:Anemia, Hemolytic, Congenital Nonspherocytic; D000818:Animals; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism; D006019:Glycolysis; D009154:Mutation; D010053:Ovary / Q000378:metabolism*; D010084:Oxidation-Reduction; D010735:Phosphoglycerate Kinase / Q000172:deficiency
D005971:Glutamates; D005973:Glutamine; D018698:Glutamic Acid; D010735:Phosphoglycerate Kinase; D005947:Glucose