title: Inactivation of simian rotavirus SA11 by chlorine, chlorine dioxide, and monochloramine.
abstract: The kinetics of inactivation of simian rotavirus SA11 by chlorine, chlorine dioxide, and monochloramine were studied at 5 degrees C with a purified preparation of single virions and a preparation of cell-associated virions. Inactivation of the virus preparations with chlorine and chlorine dioxide was studied at pH 6 and 10. The monochloramine studies were done at pH 8. With 0.5 mg of chlorine per liter at pH 6, more than 4 logs (99.99%) of the single virions were inactivated in less than 15 s. Both virus preparations were inactivated more rapidly at pH 6 than at pH 10. With chlorine dioxide, however, the opposite was true. Both virus preparations were inactivated more rapidly at pH 10 than at pH 6. With 0.5 mg of chlorine dioxide per liter at pH 10, more than 4 logs of the single-virus preparation were inactivated in less than 15 s. The cell-associated virus was more resistant to inactivation by the three disinfectants than was the preparation of single virions. Chlorine and chlorine dioxide, each at a concentration of 0.5 mg/liter and at pH 6 and 10, respectively, inactivated 99% of both virus preparations within 4 min. Monochloramine at a concentration of 10 mg/liter and at pH 8 required more than 6 h for the same amount of inactivation.
mesh_terms: D000818:Animals; D002460:Cell Line; D002700:Chloramines / Q000494:pharmacology*; D002713:Chlorine / Q000494:pharmacology*; D017606:Chlorine Compounds; D002522:Chlorocebus aethiops; D007668:Kidney; D007700:Kinetics; D008854:Microscopy, Electron; D010087:Oxides / Q000494:pharmacology*; D012401:Rotavirus / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002700:Chloramines; D017606:Chlorine Compounds; D010087:Oxides; D002713:Chlorine; C025109:chlorine dioxide; C030816:chloramine