Growth-inhibition of leukemia L-1210 ascites tumor by combination of carrageenan and mitomycin C in vivo.
A purified i-carrageenan prepared by means of pronase digestion and fractional precipitation with KCl was investigated for its growth-inhibitory effect on mouse leukemia L-1210 transplanted in (C57BL/6 x DBA/2)F1 (BDF1) mice. This carrageenan specimen was not growth-inhibitory on this tumor when it was administered alone in a dose of 0.1 to 10 mg/kg/d, 4 times. However, in combination, with a small amount of mitomycin C, 0.25 mg/kg/d, a remarkable tumor growth-inhibition has manifested affording a complete regression of this tumor. Acid phosphatase alpha-galactosidase and active oxygen generation activities of the peritoneal exudate cells in the carrageenan-administered mice were remarkably higher than those of the control mice. Therefore, the booster effect of this carrageenan preparation on antitumor activity of mitomycin C is assumed to be associated with the inflammatory activity of this sulfated polysaccharide.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology*; D005122:Exudates and Transudates / Q000201:enzymology; D007700:Kinetics; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D014343:Trypan Blue / Q000494:pharmacology; D000519:alpha-Galactosidase / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D002351:Carrageenan; D000135:Acid Phosphatase; D000519:alpha-Galactosidase; D014343:Trypan Blue