Changes in myelin and axonal proteins in CaCl2-induced myelopathy in rat spinal cord.
Calcium-induced myelopathy was produced in rats by dripping 1.0 ml of a 10% solution of CaCl2 at pH 7.4 upon exposed spinal cord. Changes in spinal cord proteins were examined following application of calcium. Analysis of proteins by SDS-PAGE revealed progressive losses of neurofilament, microtubular, and glial filament proteins over a period of 8 hours to 5 days. Large losses of myelin proteins were also evident. The protein alterations observed correlate well with ultrastructural changes and resemble those previously found with physical trauma. These observations indicate that Ca2+ plays a pivotal role, possibly by activating proteinase(s), in the degeneration of axons and myelin sheath in both Ca2+-induced myelopathy and spinal cord injury.
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002122:Calcium Chloride; D002154:Calpain / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008868:Microtubule Proteins / Q000378:metabolism*; D009185:Myelin Proteins / Q000378:metabolism*; D009410:Nerve Degeneration; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000187:drug effects; D013118:Spinal Cord Diseases / Q000139:chemically induced* / Q000378:metabolism; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D008868:Microtubule Proteins; D009185:Myelin Proteins; D016900:Neurofilament Proteins; D002154:Calpain; D002122:Calcium Chloride