Alternative splicing in the 5' moiety of the H-2Kd gene transcript.
The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They correspond to two previously characterized cDNA clones isolated from DBA/2 mouse liver RNA, leading to the conclusion that the H-2Kd gene gives rise to two distinct transcripts through an alternate use of splicing sites. The non-canonical RNA potentially encodes a so far undescribed H-2Kd-like molecule. It is present in all tissues tested (liver, spleen, thymus, kidney) albeit in lower amounts (approximately 10-fold less) than the canonical RNA coding for H-2Kd.
D000818:Animals; D004247:DNA / Q000032:analysis; D004720:Endonucleases / Q000378:metabolism; D006183:H-2 Antigens / Q000235:genetics*; D008099:Liver / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D012326:RNA Splicing; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D006183:H-2 Antigens; C024755:H-2K(K) antigen; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases