[Immunochemical evidence for the specific binding of putrescine to plasma proteins including fibronectin].
Human plasma contains proteins capable of binding 14C putrescine by the action of Ca++ activated transglutaminase. These proteins have molecular weights from 32 to 220 K and above. One of these (with a molecular weight of 220 K) has been identified as fibronectin by the use of an antifibronectin antiserum. Evidence for a protein with a molecular weight identical to that of fibronectin has been obtained on PAGE analysis of the precipitate formed on incubating human serum with antipolyamine antiserum.
D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000097:blood*; D006801:Humans; D007106:Immune Sera; D008970:Molecular Weight; D011073:Polyamines / Q000276:immunology; D011700:Putrescine / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000097:blood
D002352:Carrier Proteins; D005353:Fibronectins; D007106:Immune Sera; D011073:Polyamines; D005723:gamma-Glutamyltransferase; D013917:Thrombin; D002118:Calcium; D011700:Putrescine
Mise en évidence immunochimique de l'attachement spécifique de la putrescine aux proteines plasmatiques dont la fibronectine.