title: Changes in albumoid protein during cataract formation.
abstract: The water-insoluble albumoid protein of the human cataractous lenses was extracted in Tris/glycine buffer (pH 8.2). Subsequent homogenization in 8 M urea containing 1% beta-mercaptoethanol gave three major fractions: urea soluble fraction (USF), urea beta-mercaptoethanol-soluble fraction (U-beta-merc-IF). and urea beta-mercaptoethanol-soluble fraction (U-beta-merc-SF). The urea beta-mercaptoethanol-soluble fraction (U-beta-merc-SF) constitutes the pigmented protein of the human cataractous lenses. The amino-acid composition indicates the presence of tryptophan, high concentration of cysteine and low concentration of lysine and methionine. The brown protein is an aggregate of protein subunits which may be a protein chromophore, that directs all possible adaptive phenomenon during vision.
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000418:Albumins / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D002386:Cataract / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008875:Middle Aged; D012995:Solubility
chemical_list: D000418:Albumins; D000596:Amino Acids; D003459:Crystallins; D005136:Eye Proteins