A filter paper sandwich method using small volumes of reagents for the detection of antigens electrophoretically transferred onto nitrocellulose.
A method is described which allows antigens electrophoretically 'blotted' onto nitrocellulose to be probed using small volumes (less than 0.3 ml) of antibody solutions. Reagent volumes required for this method are more than 10-fold lower than the minimum volumes attainable using either custom made or commercial incubation trays. The procedure is simple and economical and should be applicable to any situation where it is desirable to either conserve or reduce the amount of screening reagent used.
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008565:Membrane Proteins / Q000032:analysis; D008722:Methods; D008871:Microvilli / Q000032:analysis; D010209:Paper; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D011990:Receptors, Transferrin; D014168:Transferrin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000941:Antigens; D000954:Antigens, Surface; D007202:Indicators and Reagents; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin; D003101:Collodion