title: Effects of high glucose and high lard diets on the activities of rat liver glycosyltransferases.
abstract: The activities of several enzymes involved in glycoprotein synthesis were measured in the livers of rats (L) fed diets with 67% of calories as lard and compared with those of rats (G) fed 67% glucose diets for 5-9 days. Glucosamine synthetase activity was not influenced by diet, but the activities of UDP-N-acetylglucosaminyl, galactosyl and sialyltransferases were significantly greater in the livers of the rats fed the glucose diet than in L rat livers. The content of UDP-N-acetylglucosamine was also higher in G livers than in the L group. Activities of glycosidases and of sugar nucleotide pyrophosphatases and phosphatases were the same on both diets. Serum total hexosamine was higher in L than in G rats. These findings are discussed in relation to earlier reports that liver plasma membranes from G rats contained more glycoprotein carbohydrate than L membranes.
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005944:Glucosamine / Q000097:blood; D005947:Glucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D017351:N-Acetylglucosaminyltransferases; D011755:Pyrophosphatases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012799:Sialyltransferases / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D014539:Uridine Diphosphate Sugars; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D012799:Sialyltransferases; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D011755:Pyrophosphatases; D005947:Glucose; D005944:Glucosamine