3',5'-Cyclic nucleotide phosphodiesterase activity of the sulphatase A of ox liver.
The sulphatase A (aryl-sulphate sulphohydrolase, EC 3.1.6.1) of ox liver hydrolyses adenosine 3',5'-monophosphate (cyclic AMP) to adenosine 5'-phosphate at an optimum pH of approx. 4.3, close that for the hydrolysis of cerebroside sulphate, a physiological substrate for sulphatase A. The Km is 11.6 mM for cyclic AMP. On polyacrylamide gel electrophoresis sulphatase A migrates as a single protein band which coincides with both the arylsulphatase and phosphodiesterase activities, suggesting that these are due to a single protein. Cyclic AMP competitively inhibits the arylsulphatase activity of sulphatase A, showing that both activities are associated with a single active site on the enzyme. sulphatase A also hydrolyses guanosine 3',5'-monophosphate, but not uridine 3',5'-monophosphate nor adenosine 2',3'-monophosphate.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000302:isolation & purification / Q000378:metabolism*; D000227:Adenine Nucleotides; D000818:Animals; D002417:Cattle; D002553:Cerebroside-Sulfatase / Q000302:isolation & purification / Q000378:metabolism*; D000242:Cyclic AMP; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity; D013429:Sulfatases / Q000378:metabolism*
D000227:Adenine Nucleotides; D010710:Phosphates; C010565:2',3'-cyclic AMP; D000242:Cyclic AMP; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D013429:Sulfatases; D002553:Cerebroside-Sulfatase