Hormone content of mouse pancreatic islets subjected to different in vivo and in vitro functional demands.
Mice treated for 4 days with tolbutamide displayed decreased serum glucose values with a concomitant decrease of their islet insulin content. Mouse islets cultured for 1 week at a low (3 mM) or a high (28 mM) glucose concentration contained less insulin than non-cultured islets and islets cultured at a medium (11 mM) glucose concentration. All groups of cultured islets contained more glucagon than non-cultured islets. The somatostatin content of high- and medium-glucose cultured islets was higher than that of freshly isolated islets.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006728:Hormones / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D013004:Somatostatin / Q000378:metabolism; D013217:Starvation; D014044:Tolbutamide / Q000494:pharmacology
D001786:Blood Glucose; D006728:Hormones; D007328:Insulin; D013004:Somatostatin; D005934:Glucagon; D014044:Tolbutamide; D005947:Glucose