Simplified competitive protein binding assay for 1,25-dihydroxycholecalciferol, the most active metabolite of vitamin D.
Simplified competitive protein binding assay for determination of 1,25-dihydroxycholecalciferol, with chicken intestinal cytosol receptor as binding protein and tritiated 1,25-dihydroxycholecalciferol as a competitive radioligand was developed using the same outline procedure as for determination of the other biologically active metabolites of cholecalciferol. Binding characteristics (binding capacity and affinity) of a cytosol receptor were determined.
D000818:Animals; D001667:Binding, Competitive; D002117:Calcitriol / Q000032:analysis* / Q000378:metabolism; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D011485:Protein Binding; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol