title: Physiological characterization of a Neurospora crassa mutant with impaired regulation of nitrate reductase.
abstract: This report describes the isolation and characterization of a Neurospora crassa mutant with an impaired regulation of nitrate reductase. Glutamine, which prevents the induction of nitrate reductase in N. crassa, did so relatively ineffectively in this mutant. The mutation did not affect the regulation of all enzymes regulated by "nitrogen metabolite regulation"; it did affect the regulation of nitrate reductase, nitrite reductase, histidase, and acetamidase, as well as that of thiourea sensitivity. The mutation was not allelic with nit-2, the gene controlling a general positive effector of nitrogen metabolite-regulated enzyme formation.
mesh_terms: D004790:Enzyme Induction; D005786:Gene Expression Regulation; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D005973:Glutamine / Q000494:pharmacology*; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D009565:Nitrate Reductases / Q000096:biosynthesis*; D009566:Nitrates
chemical_list: D009566:Nitrates; D005973:Glutamine; D005969:Glutamate Dehydrogenase; D009565:Nitrate Reductases; D005974:Glutamate-Ammonia Ligase