title: A comparison of test materials for differentiating avian leukosis virus group-specific antigens of exogenous and endogenous origin.
abstract: Three groups of pullets--those lacking endogenous viral (ev) genes, those carrying ev3, which codes for avian leukosis virus (ALV) group-specific (gs) antigen but not complete virus, and those carrying ev2, which codes for complete endogenous virus--were reared to maturity free of exogenous ALV infection or reared separately after inoculation at 1 day with ALV. The enzyme-linked immunosorbent assay (ELISA) was used to detect gs antigen in feather pulp, cloacal swabs, sera, white blood cells, and albumens from the pullets and in embryos, combs, and meconia from their progeny. These results were used to identify methods to distinguish between endogenous ALV expression and exogenous ALV infection. Although the frequency and levels of gs antigen detection were higher in most of the ALV-positive than in ev-positive ALV-negative materials, albumens and cloacal swabs had the lowest frequency of gs antigen detection in the ev-positive ALV-negative materials. These two materials had a further advantage in that detection of gs antigen in them has been shown to be highly correlated with congenital transmission. Further studies using ELISA absorbance values and titer to quantitate gs antigen showed that ev-positive ALV-negative albumens had much lower levels of gs antigen than ALV-positive albumens. The same criteria were not useful for distinguishing cloacal swabs of these two types. We conclude that in these lines, high levels of gs antigen in albumen is a sensitive and practical means of identifying dams congenitally transmitting ALV, because there is a very low frequency of "false positives" due to endogenous gs antigen in this material.
mesh_terms: D000418:Albumins / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D001354:Avian Leukosis Virus / Q000235:genetics / Q000276:immunology*; D002642:Chick Embryo / Q000276:immunology; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D002988:Cloaca / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005241:Feathers / Q000276:immunology; D005260:Female; D005814:Genes, Viral; D007962:Leukocytes / Q000276:immunology
chemical_list: D000418:Albumins; D000956:Antigens, Viral