Comparison of cumene hydroperoxide- and NADPH/Fe3+/ADP-induced lipid peroxidation in heart and liver submitochondrial particles. Mechanisms of protection by succinate.
The NADPH/Fe3+ /ADP system stimulates lipid peroxidation both in rat liver and bovine heart submitochondrial particles, while cumene hydroperoxide is active only in rat liver submitochondrial particles. The lack of a peroxidizing effect of cumene hydroperoxide in heart submitochondrial particles was related to the absence of cytochrome P-450. When ubiquinones are extracted from rat liver and bovine heart submitochondrial particles, succinate can still partially protect the cumene hydroperoxide-induced lipid peroxidation but not the peroxidation induced by NADPH/Fe3+ /ADP. The protective effect of succinate in lipid peroxidation was referred either to the reduction of ubiquinones that can act as antioxidants in the NADPH/Fe3+ /ADP system, or to the reduction of cytochrome P-450 that acts as a peroxidase in the cumene hydroperoxide system.
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D002417:Cattle; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism*; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism
D001555:Benzene Derivatives; D008054:Lipid Peroxides; D009249:NADP; D000244:Adenosine Diphosphate; D007501:Iron; D013384:Succinate Cytochrome c Oxidoreductase; C007164:cumene hydroperoxide