A kinetic fluorimetric assay for the measurement of angiotensin-converting enzyme in human serum.
The development and assay performance of a direct kinetic fluorimetric assay for angiotensin-converting enzyme is described. It is based on the substrate developed by Carmel et al. (Clin Chim Acta 1979; 93: 215-20), and applied to a microcentrifugal analyser with fluorescence optics. The method is sufficiently sensitive and precise to detect both normal and pathological activities; the method is linear up to at least three times the upper limit of the reference range. The method correlates well with an established method. Because of interference, the method is unsuitable for use with icteric or haemolysed samples.
D000618:Aminohippuric Acids / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005470:Fluorometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009842:Oligopeptides / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D015032:Zinc / Q000378:metabolism
D000618:Aminohippuric Acids; D002712:Chlorides; D009842:Oligopeptides; C033412:2-aminohippuric acid; C043712:2-aminobenzoylglycyl-4-nitrophenylalanyl-proline; D004492:Edetic Acid; D007703:Peptidyl-Dipeptidase A; D015032:Zinc