In vitro degradation of guanosine 3',5'-bis(diphosphate) [ppGpp] by the spoT gene product [ppGppase] from auxotrophic strains of Escherichia coli: effects of various antibiotics and drugs.
The enzyme specifically hydrolyzing guanosine 3',5'-bis(diphosphate) [ppGpp] has been isolated from the ribosomal fraction of Escherichia coli; it released pyrophosphate from the 3'-position of ppGpp. The effects of various drugs and antibiotics known to interfere with protein and/or RNA synthesis were investigated in the ppGpp degrading reaction. It was determined that tetracycline, chlorotetracycline, and thiostrepton strongly inhibited the reaction, whereas levallorphan gave a moderate inhibition. Only the tetracycline-mediated inhibition could be reversed by manganese ions. Oxytetracycline, rifampicin, fusidic acid, kirromycin, streptomycin, puromycin, chloramphenicol, and morphine did not inhibit the decay reaction.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D005672:Fusidic Acid / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000378:metabolism*; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D007977:Levallorphan / Q000494:pharmacology*; D009020:Morphine / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D011755:Pyrophosphatases / Q000378:metabolism*; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013754:Tetracyclines / Q000494:pharmacology; D013883:Thiostrepton / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D006150:Guanine Nucleotides; D013754:Tetracyclines; D006159:Guanosine Tetraphosphate; D007977:Levallorphan; D011691:Puromycin; D005672:Fusidic Acid; D002701:Chloramphenicol; D009020:Morphine; C027627:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase; D011755:Pyrophosphatases; D013752:Tetracycline; D013883:Thiostrepton; D013307:Streptomycin