A quantitative beta-galactosidase alpha-complementation assay for fusion proteins containing human insulin B-chain peptides.
The alpha-complementation reaction was adapted as a quantitative assay for fusion proteins consisting of the B chain of human insulin fused to the C terminus of the truncated beta-galactosidase.
D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D007328:Insulin / Q000235:genetics*; D046911:Macromolecular Substances; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics*
D007328:Insulin; D046911:Macromolecular Substances; D004262:DNA Restriction Enzymes; D005696:Galactosidases; D001616:beta-Galactosidase