Simultaneous determination of guanine nucleotides in rat brain by high-performance liquid chromatography with dual-electrochemical detection.
A new, sensitive, and specific assay method for guanine nucleotides using high-performance liquid chromatography with dual-electrochemical detection was developed. GTP, GDP, GMP, and cyclic GMP were separated with reversed-phase "ion-pair" chromatography and detected by a dual-electrochemical detector. Only guanine nucleotides among all purine and pyrimidine nucleotides responded to the electrochemical detector at 0.95 V. The peak heights for these guanine nucleotides were linear at concentrations between 0.5 pmol and 1 nmol. The regional distribution of these guanine nucleotides in the rat brain was studied by this new assay method.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006152:Cyclic GMP / Q000032:analysis; D004563:Electrochemistry; D006150:Guanine Nucleotides / Q000032:analysis*; D006153:Guanosine Diphosphate / Q000032:analysis; D006157:Guanosine Monophosphate / Q000032:analysis; D006160:Guanosine Triphosphate / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006150:Guanine Nucleotides; D006153:Guanosine Diphosphate; D006157:Guanosine Monophosphate; D006160:Guanosine Triphosphate; D006152:Cyclic GMP