A simple and rapid method to remove light chain phosphatase from chicken gizzard myosin.
A simple and rapid method to remove myosin light chain phosphatase from gizzard myosin using hydroxyapatite chromatography was developed. Stable phosphorylated myosin without light chain phosphatase and kinase was also prepared by the same procedure. A convenient method using the urea gel system to detect minor contamination by phosphatase of myosin is also described.
D000818:Animals; D002645:Chickens; D002845:Chromatography; D017886:Durapatite; D005895:Gizzard, Avian / Q000201:enzymology*; D006882:Hydroxyapatites; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins / Q000302:isolation & purification*; D010749:Phosphoprotein Phosphatases / Q000302:isolation & purification*; D011494:Protein Kinases / Q000302:isolation & purification
D006882:Hydroxyapatites; D017886:Durapatite; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins