Leukotriene D: a slow reacting substance from rat basophilic leukemia cells.
A slow reacting substance produced by rat basophilic leukemia cells, treated with ionophore A23187, was characterized by spectroscopic methods, enzymatic conversions, and chemical degradations as 5-hydroxy-6-S-cysteinylglycyl 7,9,11,14-eicosatetraenoic acid (leukotriene D). gamma-Glutamyltranspeptidase [gamma-glutamyltransferase; (5-glutamyl)-peptide: amino-acid 5-glutamyltransferase, EC 2.3.2.2] converted leukotriene C to a product identical to leukotriene D. This suggests that the stereochemistry of the arachidonyl moiety of leukotrienes C and D is the same [5(S)-6(R)-7,9-trans-11,14-cis]. Leukotriene D induces a faster contraction and, on a molar basis, is more potent than leukotriene C in the isolated guinea pig ileum bioassay.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis; D012898:Autacoids / Q000032:analysis; D001491:Basophils / Q000032:analysis*; D007938:Leukemia / Q000032:analysis*; D008084:Lipoxygenase / Q000378:metabolism; D051381:Rats; D013189:SRS-A; D013056:Spectrophotometry, Ultraviolet; D013455:Sulfur
D000596:Amino Acids; D001095:Arachidonic Acids; D012898:Autacoids; D013189:SRS-A; D013455:Sulfur; D008084:Lipoxygenase