[Effect of prostaglandin E1 on the cyclic adenosine-3',5'--monophosphate system and radiosensitivity of B-82 cells cultured in vitro].
The cyclic AMP (cAMP) system of mice fibroblasts L, clone B-82 has been studied. B-82 cells possess receptors to prostaglandin E1 (PGE1) and are deficient in beta-adrenoreceptors. It has been shown that 10 mM NaF, 1.10(-4) M GTP and 1.10(-5) M PGE1 stimulate adenylate cyclase of B-82 cells. PGE1 causes increase in the intracellular content of cAMP and protects B-82 cells against irradiation. Specifict beta-agonist isoproterenol fails to modulate adenylate cyclase activity and does not change cAMP content in B-82 cells. Isoproterenol has no effect on the B-82 cells radiosensitivity, although it is known to be a potent radioprotector. It is presumed that the stimulation of cAMP system by radioprotector is necessary for the performance of radioprotective effect.
D000262:Adenylyl Cyclases / Q000378:metabolism / Q000528:radiation effects; D000527:Alprostadil; D000818:Animals; D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000242:Cyclic AMP / Q000378:metabolism* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007739:L Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D011458:Prostaglandins E / Q000494:pharmacology*; D011836:Radiation Tolerance; D012969:Sodium Fluoride / Q000494:pharmacology
D011458:Prostaglandins E; D006160:Guanosine Triphosphate; D012969:Sodium Fluoride; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000527:Alprostadil; D007545:Isoproterenol
Vliianie prostaglandina E1 na sistemu tsiklicheskogo adenozin--3',5'-monofosfata i radiochuvstvitel'nost' kletok B-82, kul'tiviruemykh in vitro.