Comparison of the coverslip and the discontinuous Percoll density gradient methods of enucleation of mouse cells.
Two methods of enucleation of LB 10 cells, a subline of mouse L cells, were used: the method of enucleation of cells growing in monolayers, and the newly improved method of enucleation in discontinuous Percoll gradients. The second method was more effective and, as shown by incorporation of 3H-lysine, protein synthesis in cytoplasts was prolonged twice when compared with that in cytoplasts obtained by the coverslip method.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000379:methods*; D003593:Cytoplasm / Q000378:metabolism; D007739:L Cells; D051379:Mice; D011205:Povidone; D014176:Protein Biosynthesis; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone