Release of superoxide anion from resident and activated mouse peritoneal macrophages infected with Mycobacterium intracellulare.
Phagocytosis of Mycobacterium intracellulare triggered the release of superoxide anion (O-2) from mouse peritoneal macrophages; the amount release from BCG-activated cells was significantly greater than that from resident cells. Superoxide anion release was proportional to the number of phagocytes ingesting bacilli. An optimal phagocytic time course of 4 h was observed when estimating O-2 release consequent to bacterial challenge. Also, an inverse relationship between the amount of O-2 released and the virulence of M. intracellulare in mice was shown. Transparent and rough colony variants triggered the release of significantly lower amounts of O-2 as compared with the opaque and smooth colony variants. Serovars 4 and 8, which cause a disease with a poor prognosis in humans, triggered significantly less O-2 than did serovars 9, 12, 13, 14, 16, 18, and 19, which respond more favorably to chemotherapeutic regimens.
D000818:Animals; D001500:BCG Vaccine; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D009161:Mycobacterium / Q000276:immunology*; D009170:Nontuberculous Mycobacteria / Q000276:immunology* / Q000472:pathogenicity; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism*; D013997:Time Factors; D014774:Virulence
D001500:BCG Vaccine; D013481:Superoxides