A lipolytic factor from the submaxillary salivary glands of the rat.
In 1978 it was observed that an aqueous extract of the submaxillary salivary glands of rats contained a protein substance which was able in vitro to mobilize free fatty acids and glycerol from the adipose tissue of rat epididymis. Using a Sephadex G-100 column it was possible to demonstrate in the aqueous extract of rat submaxillary salivary glands three protein fractions with molecular weights 72 000 D, 52 000 D and 14 000 D. Each of these fractions could stimulate lipolysis and the optimal protein concentration in the incubation fluid was 10(-6)M. In an alkaline medium the protein fractions exerted a greater lipolytic effect. In relation to adrenaline, ACTH, glucagon, the lipolytic effect of the studied fractions was about 30% lower.
D000273:Adipose Tissue / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002850:Chromatography, Gel; D004822:Epididymis / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D008053:Lipid Mobilization; D008066:Lipolysis; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D013363:Submandibular Gland / Q000032:analysis*; D014020:Tissue Extracts / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D012471:Salivary Proteins and Peptides; D014020:Tissue Extracts; D000324:Adrenocorticotropic Hormone; D005934:Glucagon; D005990:Glycerol; D004837:Epinephrine