title: Use of egg yolk in serological tests (ELISA and HI) to detect antibody to Newcastle disease, infectious bronchitis, and Mycoplasma gallisepticum.
abstract: Serum and yolks from commercial flocks and from hens exposed to Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and Mycoplasma gallisepticum (MG) were tested for immunoglobulin G antibody by the enzyme-linked immunosorbent assay (ELISA) and the hemagglutination-inhibition (HI) test. Yolks prepared by chloroform extraction and low-speed centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the ELISA for NDV, IBV, and MG and by HI test for NDV.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens / Q000276:immunology*; D003332:Coronaviridae / Q000276:immunology*; D004530:Egg Yolk; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006386:Hemagglutination Tests / Q000379:methods; D001351:Infectious bronchitis virus / Q000276:immunology*; D009174:Mycoplasma / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral