title: The application of HPLC methodology for the analysis of receptor mediated changes in phosphoinositide metabolism.
abstract: HPLC methodology has found wide application in analytical problems in biochemistry. To study the metabolism of phosphatidylinositol and its regulation by receptor mediated events, HPLC could be a valuable technique. It has been recently demonstrated that a variety of hormones and neurotransmittors act to stimulate hydrolysis of phosphoinositides by a phospholipase C. To monitor this reaction, we have analysed the formation of radiolabelled inositol phosphates from phosphoinositides. The present paper describes a rapid HPLC procedure, to separate inositol phosphates from myo-inositol, which could be used in pharmacological studies of receptors linked to phosphoinositide hydrolysis. The potential of the application of HPLC to the analysis of the phospholipids involved is discussed.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D007294:Inositol / Q000032:analysis; D007295:Inositol Phosphates / Q000032:analysis / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000032:analysis / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000502:physiology*; D013403:Sugar Phosphates / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
chemical_list: D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D013403:Sugar Phosphates; D007294:Inositol; D013973:Thyrotropin-Releasing Hormone