Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells.
Microspectrofluorometry was used to examine intracellular free calcium changes in single NG108-15 neurons loaded with the Ca2+ sensitive probe, quin2. The changes in intracellular free Ca2+ were induced either by depolarizing cells with high K+ or veratridine or by the addition of ionomycin. Ca2+-free medium and various inorganic and organic calcium-channel blocking agents blocked changes in intracellular free Ca2+ levels, indicating that Ca2+ entry is most likely through voltage-sensitive calcium channels.
D002118:Calcium / Q000032:analysis*; D002460:Cell Line; D003035:Cobalt / Q000494:pharmacology; D005711:Gallopamil / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects; D007476:Ionophores / Q000494:pharmacology; D008564:Membrane Potentials; D009474:Neurons / Q000032:analysis*; D009539:Nicotinic Acids / Q000494:pharmacology; D009553:Nimodipine; D011188:Potassium / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
D007473:Ion Channels; D007476:Ionophores; D009539:Nicotinic Acids; D005711:Gallopamil; D003035:Cobalt; D009553:Nimodipine; D014701:Veratridine; D011188:Potassium; D002118:Calcium