Mechanism for exclusion of 5-fluorouracil from DNA.
5-Fluorodeoxyuridine 5'-triphosphate is hydrolyzed by the enzyme deoxyuridine triphosphate diphosphohydrolase (EC 3.6.1.23). Uracil-DNA glycosylase removes 5-fluorouracil (FUra) from FUra-containing DNA similar to its removal of uracil from uracil-containing DNA. The absence of FUra in DNA following exposure of cells to FUra can be explained by the activities of these two enzymes.
D004247:DNA / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D005468:Fluorodeoxyuridylate / Q000378:metabolism; D005472:Fluorouracil / Q000378:metabolism*; D066298:In Vitro Techniques; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D014498:Uracil
D003856:Deoxyuracil Nucleotides; D005468:Fluorodeoxyuridylate; D014498:Uracil; D004247:DNA; D009699:N-Glycosyl Hydrolases; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase; D005472:Fluorouracil