Single channel recordings from basolateral and apical membranes of renal proximal tubules.
A new method is described, which enables the recording of single ionic channels from the basolateral as well as the luminal membrane of renal proximal tubules with the patch-clamp technique. Segments of late proximal tubules of rabbit kidney are cannulated and perfused from one end. The other end is open and freely accessible to a patch pipette. The patch electrode can be moved against lateral cell membranes or can be inserted through the open end into the lumen and brought to contact with the brush-border membrane. Both, in the basolateral as well as in the luminal membrane, giga seals can be achieved. In both membranes, K+ selective channels could be identified.
D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D004848:Epithelium / Q000502:physiology; D007473:Ion Channels / Q000502:physiology*; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D011188:Potassium / Q000378:metabolism*; D011817:Rabbits
D007473:Ion Channels; D011188:Potassium