Rabbit liver microsomal metabolism of enclomiphene.
Metabolism of the triarylethylene antiestrogen enclomiphene (EC) has been studied in rabbit liver. Incubation of EC with microsomes in the presence of NADPH gave two metabolites, EC-1 and EC-2, as determined by thin-layer chromatography (TLC). EC-1 had mass-spectral features that were identical to those of synthetic desethylenclomiphene, and was indistinguishable from this compound by TLC and gas-liquid chromatography (GLC). EC-2 was similarly identical to synthetic 4-hydroxyclomiphene, but was separable from 3-hydroxyclomiphene by TLC ang GLC. EC-2 methyl ether was homogeneous with 4-methoxyclomiphene, but not with 4'-methoxyclomiphene, in GLC. These results suggest that enclomiphene is metabolized in a manner qualitatively similar to that reported for other structurally related antiestrogens.
D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D002996:Clomiphene / Q000031:analogs & derivatives* / Q000378:metabolism; D064695:Enclomiphene; D007536:Isomerism; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D011817:Rabbits; D013329:Structure-Activity Relationship
D002996:Clomiphene; D064695:Enclomiphene