Isolation and characterization of proteases from Bacteroides melaninogenicus.
We isolated two types of intracellular proteases from a strain of Bacteroides melaninogenicus. These enzymes were extracted from cells by ultrasonic treatment and were partially purified. These two enzymes (proteases I and II) differed in molecular weight, heat stability, sensitivity to reducing agents, Km value, and optimum pH for activity.
D001439:Bacteroides / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D001443:Prevotella melaninogenica / Q000201:enzymology*; D013379:Substrate Specificity; D013864:Thioglycolates / Q000494:pharmacology
D002413:Cations, Divalent; D013864:Thioglycolates; D010447:Peptide Hydrolases; D004229:Dithiothreitol