Basement membrane laminin and type IV collagen in various benign and malignant adnexal tumors of the skin: an immunohistochemical study.
Thirty benign and seven malignant adnexal tumors of the skin and one lymph node metastasis were stained for laminin and type IV collagen with rabbit antibodies against the human basement membrane (BM) proteins using the immunoperoxidase technique. Fifteen of the benign sweat gland, sebaceous gland, and hair follicle tumors showed a continuous and distinct BM around the tumor aggregates. The cylindromas and eccrine spiradenomas seemed to produce excessive amounts of BM material, part of which was seen as amorphic patches within the tumor cell clusters, whereas the trichofolliculomas, trichoepitheliomas, and pilomatrixomas showed an absence of BM from many areas. In syringomas, in addition to the tubular structures surrounded by a continuous BM, undifferentiated cell nests containing granular BM material were present. They probably represent primitive structures obtaining during early development into tubules. The seven malignant tumors and the only metastasis studied here all contained small, narrow strips of BM material extracellularly between the infiltrating tumor clusters. Only in two cases was faint staining for laminin found within the cells. The pepsin pretreatment of the formalin-fixed, paraffin-embedded samples had most probably degraded the intracytoplasmic BM material in most cases. The BM defects were found to be associated with malignancy and low differentiation of the adnexal skin tumors, as reported previously for other tumor types, but a partial loss of BM was also associated with high differentiation in some benign adnexal tumors.
D000236:Adenoma / Q000032:analysis / Q000648:ultrastructure; D006607:Adenoma, Sweat Gland / Q000032:analysis / Q000648:ultrastructure; D001485:Basement Membrane / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000032:analysis / Q000648:ultrastructure; D003094:Collagen / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis*; D008207:Lymphatic Metastasis; D012867:Skin / Q000473:pathology; D012871:Skin Diseases / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000032:analysis* / Q000648:ultrastructure; D013544:Sweat Gland Neoplasms / Q000032:analysis / Q000648:ultrastructure
D007797:Laminin; D003094:Collagen