Single class of muscimol binding sites in the solubilized gamma-aminobutyrate-benzodiazepine receptor complex.
The gamma-aminobutyrate-benzodiazepine receptor complex was solubilized from the rat brain and bovine cortex and partially purified by means of gel chromatography. Analysis of binding parameters of [3H]muscimol indicates that the complex includes only one class of binding sites which presumably represent an apparently low affinity class of two classes of gamma-aminobutyrate A receptors described on the brain membranes. Analysis of the [3H] flunitrazepam specific binding also shows the presence of one class of binding sites sensitive to gamma-aminobutyrate stimulation. The concentration of muscimol binding sites in the gamma-aminobutyrate-benzodiazepine receptor complex was observed to be twice as high as the concentration of benzodiazepine binding sites.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism; D002850:Chromatography, Gel; D005445:Flunitrazepam / Q000378:metabolism; D008297:Male; D009118:Muscimol / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000302:isolation & purification / Q000378:metabolism*
D010080:Oxazoles; D011963:Receptors, GABA-A; D009118:Muscimol; D005445:Flunitrazepam