The effect of local UVB skin irradiation on the rate of formazan deposition in the epidermis of hairless mice studied by means of a tetrazolium-reduction method.
One-hundred-and-twenty hairless mice were irradiated with UVB (310 nm, exposure 60 mJ/cm2) on a limited area of the dorsal skin. At different time intervals after irradiation, the rate of endogenous dehydrogenase activity per mg dry epidermis was measured by the tetrazolium reduction method. The amount of formazan deposited remained normal for 18 h, and then increased, reaching a peak significantly higher than normal at 24 h, and thereafter returned to normal. At day 8 there was a new, probably significant peak. The reaction was followed for 14 days. It was concluded that UVB irradiation provokes a period of increased formazan deposition in the epidermis, similar to what has been observed after ionizing radiation and chemical carcinogens. The validity of the tetrazolium test for skin carcinogenic irritaments was thus also confirmed.
D000818:Animals; D001391:Azo Compounds / Q000378:metabolism*; D005562:Formazans / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D013778:Tetrazolium Salts; D013997:Time Factors; D014466:Ultraviolet Rays
D001391:Azo Compounds; D005562:Formazans; D013778:Tetrazolium Salts