Identification and characterization of a beta 1-adrenergic receptor in the rat Sertoli cell.
The beta 1-adrenergic receptor of rat Sertoli cells was characterized by measurement of the ability of adrenergic receptor agonists and antagonists to stimulate cAMP accumulation in Sertoli cells from 18-day-old rats. Epinephrine, norepinephrine and isoproterenol stimulate cAMP accumulation in Sertoli cells which is not additive with maximal doses of FSH and which is age-dependent, beta-antagonists, alprenolol, hydroxybenzylpindolol or propranolol inhibit isoproterenol-induced cAMP accumulation while alpha-adrenergic antagonists have no effect. Dobutamine and soterenol stimulated cAMP accumulation to a greater extent than albuterol and metoproterenol. Finally, the stimulatory effects of isoproterenol and zinterol are both more sensitive to inhibition by beta 1-antagonists than by beta 2-antagonists. Taken together these data indicate the presence of a beta 1-adrenergic receptor in Sertoli cells which is coupled to adenylate cyclase.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011941:Receptors, Adrenergic / Q000032:analysis*; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D012708:Sertoli Cells / Q000032:analysis*
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D005640:Follicle Stimulating Hormone; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine