Determination of immunoglobulin classes of antibodies against Coxiella burnetii by protein A from Staphylococcus aureus.
Protein A from Staphylococcus aureus was used for differentiating antibodies against Coxiella burnetii according to immunoglobulin classes. Interaction of immune serum with protein A resulted in complete (within the sensitivity limits of the methods used) removal of IgG antibody and had practically no effect on the level of IgM antibody.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002846:Chromatography, Affinity; D003381:Coxiella / Q000276:immunology*; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis*; D011778:Q Fever / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D013205:Staphylococcal Protein A