title: Identification of a high-molecular-weight (greater than 400 000) protein in hyaline cartilage.
abstract: A high-molecular-weight (greater than 400 000) non-collagenous protein has been identified in normal articular cartilage from several mammalian species and in bovine tracheal cartilage. This protein is reduced by 2-mercaptoethanol to subunits with a molecular weight of 116 000, which appear to constitute approx. 2-4% of the total protein detectable by the Lowry assay in 4 M guanidinium chloride extracts of normal bovine and canine articular cartilage. Antiserum to the 116 kDa subunit protein from bovine articular cartilage cross-reacts with the intact and subunit proteins from bovine trachea and from normal canine, porcine and human articular cartilage. This protein is not found in non-cartilagenous tissues, suggesting that it is a cartilage-specific protein. We conclude that the greater than 400 kDa protein and its subunit are ubiquitous and quantitatively significant proteins in hyaline cartilage.
mesh_terms: D000328:Adult; D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis*; D002417:Cattle; D003429:Cross Reactions; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007163:Immunosorbent Techniques; D008623:Mercaptoethanol / Q000494:pharmacology; D003012:Microbial Collagenase / Q000378:metabolism; D008875:Middle Aged; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D013552:Swine
chemical_list: D011506:Proteins; D008623:Mercaptoethanol; D003012:Microbial Collagenase