Reproducibility of gel-chromatography.
We think it worthwhile to other experimenters to mention the following observations. Recently it was noted that gel chromatographic separations of the five fragments resulting from the degradation of cytochrome c by bromocyanogen became dependent on the batch number of the gel. This dependency concerns only separations of unprotected fragments, using 7% (v/v) formic acid as the eluent. The elution profile of protected compounds, which in turn run in 50% (v/v) formic acid, was unaffected.
D002850:Chromatography, Gel / Q000379:methods*; D003488:Cyanogen Bromide; D003574:Cytochrome c Group / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification*
D003574:Cytochrome c Group; D010446:Peptide Fragments; D003488:Cyanogen Bromide