Relative effects of asbestos and wollastonite on alveolar macrophages.
Rabbit alveolar macrophages were exposed in culture to chrysotile asbestos, wollastonite, or latex, and the effects on various biochemical and physiological parameters related to cellular viability and fibrogenicity were determined. Exposure of alveolar macrophages to asbestos, wollastonite, or latex for 3 d has no effect on oxygen consumption or cellular volume. However, treatment of alveolar macrophages with as little as 25 micrograms asbestos/ml for 1 d increases lysosomal enzyme release and decreases membrane integrity, i.e., decreases trypan blue exclusion and increases leakage of cytosolic enzymes. In contrast, exposure of alveolar macrophages to wollastonite or latex at 250 micrograms/ml does not induce lysosomal enzyme release or alter membrane integrity even after 3 d of exposure in culture. These data suggest that chrysotile asbestos damages rabbit alveolar macrophages, while wollastonite, a potential substitute for asbestos, is far less cytotoxic.
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017610:Calcium Compounds; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007840:Latex / Q000633:toxicity; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000187:drug effects*; D011817:Rabbits; D017640:Silicates; D012824:Silicic Acid / Q000633:toxicity*; D012822:Silicon Dioxide / Q000633:toxicity*
D017610:Calcium Compounds; D007840:Latex; D017640:Silicates; D001194:Asbestos; D012824:Silicic Acid; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase; C031293:calcium silicate