Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node.
Lymph nodes of 5 cases with non-specific lymphadenitis and of 4 cases with dermatopathic lymphadenitis were examined by the peroxidase anti-peroxidase (PAP) method and by immunoelectron microscopy using monospecific antibody against nervous system specific S-100 protein. S-100 protein was detected in dendritic shaped cells of the thymus-dependent area of non-specific lymphadenitis. The paracortical area of dermatopathic lymphadenitis showed a marked accumulation of S-100 protein-containing dendritic shaped cells. Immunoelectron micrographs revealed that S-100 protein-containing dendritic shaped cells of both non-specific and dermatopathic lymphadenitis corresponded to interdigitating reticulum cells (IRC). The detection of S-100 protein in the nucleus and on the cytoplasmic and nuclear membrane suggested that IRCs themselves synthesized S-100 protein. S-100 protein is a very useful cell marker for the identification of IRC in the lymphoid tissue.
D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000032:analysis*; D008199:Lymphadenitis / Q000473:pathology; D008854:Microscopy, Electron; D012157:Mononuclear Phagocyte System / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009685:Nuclear Envelope / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis*
D009419:Nerve Tissue Proteins; D009418:S100 Proteins