Serendipitous cloning of a mitochondrial cDNA and its polymorphism.
A cDNA clone p5x23 was isolated from a library prepared from membrane bound mRNA from a human lymphoblastoid cell line. This clone was shown to detect a restriction fragment length polymorphism with the enzyme MspI, with frequency for the minor variant of about 10% in a Caucasian population. The clone apparently only detected homozygotes, and did not hybridize to any of a panel of human-mouse somatic cell hybrids containing all human chromosomes in various combinations. Sequence analysis of the clone showed that it encoded a part of the 3' end of the human mitochondrial cytochrome b gene.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D011110:Polymorphism, Genetic
D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D004247:DNA; D004262:DNA Restriction Enzymes