Effect of thiourea on PCMBS inhibition of osmotic water transport in human red cells.
The organomercurial reagent p-chloromercuribenzene sulfonate (PCMBS) is an inhibitor of osmotic water permeability in the human red cell membrane. We have found that thiourea, when added along with PCMBS to a red cell suspension, interferes with this inhibition and at high enough concentrations prevents the inhibition from developing altogether. For a 2 mM PCMBS concentration Ki = 3 +/- 1 mM. When thiourea is added at a later time, the PCMBS inhibition, which normally takes about 20 min to develop fully, is halted and remains fixed at the value attained by that time. Thiourea also inhibits the reversal of PCMBS inhibition by a 10 mM concentration of cysteine, the half-time for reversal increasing by more than an order of magnitude when [thiourea] = 50 mM. Possible implications for the nature of the water and urea transport pathways across the red cell membrane are discussed.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology*; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D003545:Cysteine / Q000494:pharmacology; D004347:Drug Interactions; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009995:Osmosis / Q000187:drug effects; D010663:Phenylmercury Compounds / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000097:blood; D013890:Thiourea / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
D001457:Anion Exchange Protein 1, Erythrocyte; D010663:Phenylmercury Compounds; D013438:Sulfhydryl Compounds; D014867:Water; D002731:4-Chloromercuribenzenesulfonate; D013890:Thiourea; D003545:Cysteine