SMX-1 virus-induced inhibition of ecotropic and recombinant proviral sequence amplification in thymuses of AKR mice.
SMX-1 virus delays the appearance of spontaneous thymomas in AKR mice which have been inoculated as young adults by the intrathymic route. Analyses of high-molecular-weight thymus DNAs from SMX-1 virus-inoculated AKR mice indicated the absence of 3' recombinant proviral-cellular DNA junction fragments generated by EcoRI and PvuII digestion. An average of five recombinant proviral fragments were detected in DNAs from spontaneous thymomas that developed in medium-injected control mice. Preleukemic mice that amplify murine leukemia virus-related antigens on their thymocyte surface contained unintegrated proviruses in their thymus DNAs, and 2.3-kilobase EcoRI and 2.1-kilobase PvuII recombinant DNA fragments were detected.
D000818:Animals; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005784:Gene Amplification; D005814:Genes, Viral; D051379:Mice; D008806:Mice, Inbred AKR; D009693:Nucleic Acid Hybridization; D013950:Thymus Gland / Q000382:microbiology*; D013953:Thymus Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes