title: The cellular response of T and B dependent areas of the spleen after focal thermocoagulation of liver and kidney.
abstract: Using 3H-thymidine autoradiography, the cellular response of the white splenic pulp after single or repeated thermolesions on liver and kidney was studied. Sham operations served as controls, and did not induce any significant changes in the splenic white pulp. After single or repeated thermolesions, the labeling index increased distinctly in the B and T cell areas. The central T-dependent periarteriolar lymphatic sheath (PALS) showed an earlier rise of radioactively labeled lymphocytes after repeated thermolesions than after a single lesion. After a single heat coagulation of liver and kidney a dissociation of the germinal centers occurred with consecutive hyperplasia. However, compared with the splenic reaction after other surgical injuries, especially after cryosurgical operations, the response of the B-dependent splenic areas was weaker. The distinct reaction of the central T-dependent PALS supports the conception of a more prominent cell mediated immune response of the peripheral lymphatic system with temporary T cell dysfunctions. Possibly the inflammatory complications of burned patients and the delayed wound healing of thermosurgical tissue lesions may be caused by the alteration of the lymphatic system with a disturbed cooperation of T and B lymphocytes with macrophages.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001402:B-Lymphocytes; D002056:Burns / Q000473:pathology*; D002455:Cell Division; D005260:Female; D006965:Hyperplasia; D007668:Kidney / Q000293:injuries*; D008099:Liver / Q000293:injuries*; D051381:Rats; D013154:Spleen / Q000473:pathology*; D013601:T-Lymphocytes