[Induction, characterization and isolation of preneoplastic hepatocytes following combined treatment with 2-acetaminofluorene and ferric nitrilotriacetate].
Daily administration of ferric nitrilotriacetate (5 ml/Kg b.w./day i.p.) induces in a few weeks liver siderosis; continuous treatment of the same animals with the carcinogen 2-Acetaminofluorene (300 mg/Kg of diet) results in the appearance of several iron devoid hyperplastic areas or nodules, where enzyme histochemistry reveals gamma-GT activity as well as G-6-Pase disappearance. The perfusion of the liver with calcium-free Hanks solution plus collagenase allows an easy isolation of the hepatocytes, whereas a partial separation of the different kinds of cells can be achieved by isopycnic gradient sedimentation.
D015073:2-Acetylaminofluorene / Q000494:pharmacology*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002500:Centrifugation, Isopycnic; D005290:Ferric Compounds / Q000494:pharmacology*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007501:Iron / Q000032:analysis / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005290:Ferric Compounds; D015073:2-Acetylaminofluorene; D007501:Iron; D005723:gamma-Glutamyltransferase; D005952:Glucose-6-Phosphatase; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate
Induzione, caratterizzazione ed isolamento di epatociti preneoplastici dopo trattamento combinato con 2-acetaminofluorene e nitrilotriacetato ferrico.