Radiation-induced formation of thymine-thymine crosslinks.
The formation of dimers upon gamma-irradiation of thymine, thymidine and thymidine-5'-monophosphate in N2O-saturated aqueous solution is reported. A method using capillary gas chromatography-mass spectrometry was developed for their separation and identification. Quantitative measurements reveal that about 50 per cent of OH adduct radicals of thymine undergo dimerization.
D003037:Cobalt Radioisotopes; D005720:Gamma Rays; D008401:Gas Chromatography-Mass Spectrometry; D006878:Hydroxides; D017665:Hydroxyl Radical; D011854:Radiochemistry; D012996:Solutions; D013936:Thymidine / Q000528:radiation effects*; D013938:Thymidine Monophosphate / Q000528:radiation effects*; D013941:Thymine / Q000528:radiation effects*; D013942:Thymine Nucleotides / Q000528:radiation effects*; D014867:Water
D003037:Cobalt Radioisotopes; D006878:Hydroxides; D012996:Solutions; D013942:Thymine Nucleotides; D014867:Water; D017665:Hydroxyl Radical; D013938:Thymidine Monophosphate; D013941:Thymine; D013936:Thymidine