Effects of high glucose and high lard diets on the activities of rat liver glycosyltransferases.
The activities of several enzymes involved in glycoprotein synthesis were measured in the livers of rats (L) fed diets with 67% of calories as lard and compared with those of rats (G) fed 67% glucose diets for 5-9 days. Glucosamine synthetase activity was not influenced by diet, but the activities of UDP-N-acetylglucosaminyl, galactosyl and sialyltransferases were significantly greater in the livers of the rats fed the glucose diet than in L rat livers. The content of UDP-N-acetylglucosamine was also higher in G livers than in the L group. Activities of glycosidases and of sugar nucleotide pyrophosphatases and phosphatases were the same on both diets. Serum total hexosamine was higher in L than in G rats. These findings are discussed in relation to earlier reports that liver plasma membranes from G rats contained more glycoprotein carbohydrate than L membranes.
D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005944:Glucosamine / Q000097:blood; D005947:Glucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D017351:N-Acetylglucosaminyltransferases; D011755:Pyrophosphatases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012799:Sialyltransferases / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
D004041:Dietary Fats; D014539:Uridine Diphosphate Sugars; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D012799:Sialyltransferases; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D011755:Pyrophosphatases; D005947:Glucose; D005944:Glucosamine