Modulation of globin gene expression by a tumor promoter during induced differentiation of Friend erythroleukemic cells.
Friend erythroleukemic cells were induced to differentiate by dimethylsulfoxide (Me2SO) in the absence or presence of the tumor promoter 12-O-tetradecanoyl-phorbol 13-acetate. The effects of the latter on the molecular parameters related to globin mRNA metabolism were examined. When differentiation was scored by benzidine staining, it had an inhibitory effect on Me2SO-treated cells. On the other hand, when differentiation was followed by determination of globin mRNA accumulation, it had a pleiotropic effect on Me2SO-treated cells. At the early phase of differentiation (2--3 days) the rate of globin mRNA accumulation was higher in the promoter-treated cells than in the control. This unexpectedly high level of accumulation was followed by a sharp reduction and most of the globin RNA sequences disappeared at later stages of differentiation (days 4--5). The reduction can be related to the effect of the promoter on the stability of globin RNA in the cytoplasm which was reduced from a half-life of 16 h to that of 8 h only. Other parameters, such as the rate of globin mRNA synthesis and its capability to serve as a template for cell-free protein synthesis were not affected by treatment with the promoter throughout the differentiation process.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005914:Globins / Q000235:genetics*; D007942:Leukemia, Experimental / Q000503:physiopathology*; D051379:Mice; D010704:Phorbols / Q000494:pharmacology*; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014908:Triticum / Q000378:metabolism
D010704:Phorbols; D012333:RNA, Messenger; D011061:Poly A; D005914:Globins; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide