[Enzymatic determination of nonesterified fatty acids in goat plasma].
Two enzymatic methods (Wako and Nippon Shoji Kaisha (NSK) for non-esterified fatty acid determination in ruminant blood plasma are tested and compared with a semiautomated conventional chemical method which is based on the measurement of complex extinction made with zinc dibenzyldithiocarbamate. A modification of enzymatic methods is proposed to improve sensitivity and accuracy for the low NEFA concentration in ruminant and especially in goat. Thus, in this procedure, with 100 microliters of plasma aliquot, NEFA are determined from 25 to 1000 mu eq/l. The coefficient of variation increases from 1 to 5% for the range 500-40 mu eq/liter of plasma NEFA. The same various NEFA of ruminant blood in aqueous solutions are quantitatively determined according to these three methods. But with linoleic and linolenic acido values and thus recovery seems to be lower with the two enzymatic methods than with the chemical one. NEFA concentrations in goat blood determined by both enzymatic Wako and chemical procedures give good agreement and a close coefficient of correlation (r = + 0.98). Nevertheless, values obtained with these two methods do not give such a closed correlation with results from the NSK method (R = + 0.86). With NSK procedure, NEFA concentrations in goat's blood are substantially less (congruent to 50%). For quantitative estimation of NEFA in goat or other ruminants, the author uses Wako procedure modification. The enzymic method has better sensitivity and precision, and it offers advantages in speed and simplicity. It would be utilized easily for a great number of determinations for routine applications.
D000214:Acyl Coenzyme A; D042962:Acyl-CoA Oxidase; D000818:Animals; D003066:Coenzyme A Ligases; D003124:Colorimetry; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D006041:Goats / Q000097:blood*; D007527:Isoenzymes; D008722:Methods; D010088:Oxidoreductases; D009195:Peroxidase; D010544:Peroxidases; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D000214:Acyl Coenzyme A; D005230:Fatty Acids, Nonesterified; D007527:Isoenzymes; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010088:Oxidoreductases; D010544:Peroxidases; D009195:Peroxidase; D042962:Acyl-CoA Oxidase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
Détermination enzymatique des acides gras non estérifiés dans le plasma de chévre.