title: Preparation and evaluation of antigens used in serological tests for caprine syncytial retrovirus antibody in sheep and goat sera.
abstract: Methods used to prepare antigens from caprine syncytial retrovirus (CSR) for use in the agarose gel immunodiffusion test (AGID) or an indirect enzyme-linked immunosorbent assay (ELISA) are described. Caprine and ovine sera were tested for antibody to CSR using the AGID test and ELISA incorporating a caprine system (CSR antigen and rabbit anti-goat IgG) or an ovine system (maedi-visna virus antigen and rabbit anti-sheep IgG). Good correlation was achieved in the results of the 3 tests when sera were devoid of antibody or were strongly positive. Variations in the results on weakly positive sera were considered to be more a matter of interpretation than due to basic differences in the reagents employed.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats / Q000276:immunology* / Q000382:microbiology; D005779:Immunodiffusion; D012190:Retroviridae / Q000276:immunology*; D012756:Sheep / Q000276:immunology*; D014790:Visna-maedi virus / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral