Isolation of proteins from crude mixtures with silica and silica-based adsorbents.
Silica and silica-based adsorbents have been used to isolate specific proteins from crude mixtures. Acid-activated Nugel silica and Nugel SP-Silica were used to extract human immune interferon from the conditioned medium of mixed lymphocyte cultures. Human interleukin-2 was extracted from the conditioned medium of Jurkat cells with LiChroprep RP-8. In each case, batch absorption was accomplished by gentle stirring in a microcarrier vessel. The adsorbate was collected by sedimentation, and either batch-eluted or packed into a column for gradient elution. A high degree of concentration and purification was achieved with a high recovery of biological activity.
D000327:Adsorption; D002460:Cell Line; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media; D006801:Humans; D007371:Interferon-gamma / Q000302:isolation & purification; D007376:Interleukin-2 / Q000302:isolation & purification; D008214:Lymphocytes / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification*; D012822:Silicon Dioxide
D003470:Culture Media; D007376:Interleukin-2; D011506:Proteins; D012822:Silicon Dioxide; D007371:Interferon-gamma