title: Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits.
abstract: Cottontail rabbits (Sylvilagus floridanus) were infected with Herpesvirus sylvilagus, and spleen cells were analyzed for the presence of virus-specific, covalently closed circular, and linear DNA molecules by a simple electrophoretic technique, followed by transfer to nitrocellulose filters and hybridization with cloned viral DNA (Gardella et al., J. Virol. 50:248-254, 1984). Approximately 0.2 copies per cell of circular DNA and 0.2 copies per cell of linear DNA were detected by hybridization with a cloned viral DNA fragment. The size of the viral DNA was estimated at ca. 158 kilobase pairs. Restriction endonuclease patterns suggested structural similarities to cottontail herpesvirus DNA.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D013154:Spleen / Q000382:microbiology*
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D004262:DNA Restriction Enzymes