Dense precipitate of brain tubulin with skeletal muscle myosin.
Purified tubulin from porcine brain formed a dense precipitate at 37 degrees C with muscle myosin filaments from rabbit skeletal muscle; this effect was greater than that with partially purified tubulin. ATP or GTP, which prevented the myosin filaments from precipitating, inhibited the formation of the dense precipitate, but did not dissociate the dense precipitate once formed. The dense precipitate was found by thin-section electron microscopy to be composed to side-by-side aggregates of myosin filaments whose projections might be decorated by tubulin. The decoration was also seen by negative-stain electron microscopy. The binding of tubulin to myosin filaments decreased the Mg2+- and Ca2+-GTPase activity of the myosin by about half, but did not affect either Mg2+- or Ca2+-ATPase activity. The binding ratio of tubulin to myosin in the presence of 5 mM MgCl2 was 2.2 mol/mol using purified tubulin and 1.8 mol/mol using partially purified tubulin. Five mM ATP and GTP in the presence of 5 mM MgCl2 decreased the tubulin binding by 1.6-2.0 and 1.1-1.3 mol/mol, respectively, when added before an encounter of tubulin with myosin filaments, but did not cause any decrease when added after such an encounter.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D020558:GTP Phosphohydrolases / Q000378:metabolism; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D013552:Swine; D014404:Tubulin / Q000302:isolation & purification
D046911:Macromolecular Substances; D014404:Tubulin; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases; D009218:Myosins