Detection of urinary metabolites of 2-acetylaminofluorene by cultured hamster embryo cells and high performance liquid chromatography.
Urine was collected from Sprague-Dawley rats injected intraperitoneally with a suspension of 2-acetylaminofluorene (AAF) or with an inert suspension vehicle. Morphological transformation was observed in clonal cultures of secondary passage hamster embryo cells (HEC) after the addition of AAF-treated rat urine or concentrated urine extracts prepared by incubating the urine with beta-glucuronidase. No morphological alteration was observed in cultures treated with urine (or urine extracts) from rats injected with the suspension vehicle alone. The presence of N-hydroxy- and ring-hydroxylated metabolites of AAF in the AAF-treated concentrated urine extract was confirmed by high-performance liquid chromatography (HPLC).
D015073:2-Acetylaminofluorene / Q000652:urine*; D000818:Animals; D001711:Biotransformation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006224:Cricetinae; D004622:Embryo, Mammalian; D005966:Glucuronidase; D006868:Hydrolysis; D008297:Male; D008647:Mesocricetus; D051381:Rats; D011919:Rats, Inbred Strains
D015073:2-Acetylaminofluorene; D005966:Glucuronidase