Use of a synthetic antigen to predetermine the responsiveness of cattle to vaccine-induced immunity.
Groups of healthy, virus-infected and leukaemic cattle were injected with synthetic multichain poly(Tyr, Glu)-poly(Ala)--poly(Lys) in order to predetermine responsiveness to vaccine-induced immunity. Immunospecific purification of antibodies, precipitin reactions and enzyme linked immunosorbent assay (ELISA) tests were carried out on sera collected at various intervals after immunization. Of the methods employed, the ELISA had the advantage of specificity, sensitivity and speed for screening the humoral immune response of cattle to antigenic stimulation.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D007114:Immunization; D007938:Leukemia / Q000276:immunology* / Q000662:veterinary; D001909:Leukemia Virus, Bovine; D010455:Peptides / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
D000941:Antigens; D010455:Peptides; D014612:Vaccines; C021759:(T,G)-A-L