Changes in morphology and in polyphosphoinositide turnover of human erythrocytes after cholesterol depletion.
Human erythrocytes were cholesterol-depleted (5-25%) by incubation with phosphatidylcholine vesicles in media containing Ca2+ at different concentrations (0, 28 nM, 5 microM or 1 mM). After removal of the vesicles, the cells were reincubated with [32P]phosphate in the same media. Control (incubated in buffer alone) and cholesterol-maintained erythrocytes (incubated with cholesterol/phosphatidylcholine vesicles) were treated similarly. Cholesterol depletion induced the conversion of the cells into stomatocytes III and spherostomatocytes and decreased the turnover rate of phosphatidylinositol phosphate and of phosphatidylinositol bisphosphate. None of these effects were observed in cholesterol-maintained cells. In cholesterol-depleted cells, they occurred without changes in the ATP specific activity or in the polyphosphoinositide concentrations. Moreover, these modifications of shape and of lipid metabolism were proportional to the extent of the cholesterol depletion and were independent of the external Ca2+ concentration. In contrast, other effects of cholesterol depletion, a decrease in the turnover rate of phosphatidic acid, a decrease in diacylglycerol and in phosphatidic acid concentrations were dependent on the external Ca2+ concentration. Thus it appears that the shape change was not correlated with a change in the concentrations of these phospholipids or of diacylglycerol and therefore cannot be explained by a bilayer couple mechanism involving these phospholipids. However, the spherostomatocytic transformation was correlated with the decrease in the turnover rate of the polyphosphoinositides, but not with the turnover rate of phosphatidic acid, suggesting a role for the turnover of the polyphosphoinositides in the maintenance of the erythrocyte shape.
D002118:Calcium / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood*; D004075:Diglycerides / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004913:Erythrocytes, Abnormal / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D008563:Membrane Lipids / Q000097:blood; D010710:Phosphates / Q000097:blood; D010712:Phosphatidic Acids / Q000097:blood; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000097:blood*; D010761:Phosphorus Radioisotopes
D004075:Diglycerides; D008081:Liposomes; D008563:Membrane Lipids; D010710:Phosphates; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D002784:Cholesterol; D002118:Calcium