Ionic channels in a line of embryonal carcinoma cells induced to undergo neuronal differentiation.
The gigaseal patch clamp technique was used to investigate the electrophysiological properties of a line of embryonal carcinoma cells (PCC4) that were induced to undergo neuronal differentiation. A large increase in number of voltage-dependent potassium and sodium channels was observed during differentiation. The pharmacology and kinetics of the macroscopic sodium and potassium currents in the differentiated cells closely resembled those of the rapid inward sodium current and the delayed rectifier, respectively. The kinetic behavior of single-channel potassium currents was consistent with the properties of the macroscopic delayed rectifier current.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D054278:Embryonal Carcinoma Stem Cells; D007473:Ion Channels / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D014411:Neoplastic Stem Cells / Q000503:physiopathology*; D009474:Neurons / Q000166:cytology*; D013234:Stem Cells / Q000503:physiopathology*; D013724:Teratoma / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure
D007473:Ion Channels