A new method for the microdissection of the human hypothalamus, with mapping of cholinergic, GABA and catecholamine systems in twelve nuclei and areas.
A new method was developed for the microdissection of the human hypothalamus, based on a local system of co-ordinates, using the fornix as an internal reference curve. Dopamine, noradrenaline, adrenaline, CAT, GAD and GABA were assayed in twelve microdissected nuclei and areas, in nine human post-mortem cases. This neurochemical study was aimed to demonstrate the validity of the dissection method, and its potential usefulness for the controlled study of groups of abnormal human cases.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001931:Brain Mapping / Q000379:methods*; D002395:Catecholamines / Q000032:analysis; D002795:Choline O-Acetyltransferase / Q000032:analysis; D004210:Dissection / Q000379:methods*; D004298:Dopamine / Q000032:analysis; D005260:Female; D005968:Glutamate Decarboxylase / Q000032:analysis; D006801:Humans; D007031:Hypothalamus / Q000032:analysis / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D018377:Neurotransmitter Agents / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D002395:Catecholamines; D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase; D004298:Dopamine