[Interaction of tertiapin, a neurotoxin from bee venom, with calmodulin].
Tertiapin, a neurotoxin from the honey bee venom, interacts specifically with calmodulin in the presence of Ca2+. The nature of this interaction was studied using calmodulin-cAMP phosphodiesterase system. Tertiapin does not affect the unstimulated basal activity of phosphodiesterase. However, it totally inhibits the enzyme-activating capacity of calmodulin. Analysis of the dose-dependent activation of phosphodiesterase by calmodulin in the presence of tertiapin indicated that inhibition is caused by the interaction of two tertiapin molecules with calmodulin (Kd 2 microM). The data obtained suggest that the toxic effect of tertiapin in nervous tissue is mediated by blockade of calmodulin function.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001514:Bee Venoms / Q000378:metabolism* / Q000494:pharmacology; D001667:Binding, Competitive; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D002417:Cattle; D004789:Enzyme Activation; D066298:In Vitro Techniques; D007700:Kinetics
D001514:Bee Venoms; D002147:Calmodulin; C029738:tertiapin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002118:Calcium
Vzaimodeístvie tertiapina, neĭrotoksina iz iada pchely, s kal'modulinom.