Production and partial characterization of interleukin 2 induced by periodic acid oxidation of lymphocyte membranes.
Murine splenocytes, when stimulated to undergo blastogenesis by H5IO6 oxidation, produced a lymphokine with a spectrum of properties identical to that generally ascribed to IL-2 released by lectin-stimulated cells. These included the sustained propagation of IL-2-dependent CT6 cells, as well as identical thermal and enzymatic stabilities. Cell membrane carbonyls generated in situ by the oxidation of cell membranes served as triggers for subsequent IL-2 production by the activated cells. Reduction of membrane carbonyls by NaBH4, and their addition reaction with NaHSO3 and NH2OH abrogated cell activation and inhibited, but did not abolish, IL-2 production. None of the specific carbonyl reagents, e.g., NaBH4, NaHSO3, and NH2OH, have by themselves induced blastogenic transformation, although they did elicit IL-2 production. It is therefore concluded that cell membrane carbonyls serve as triggers for IL-2 production by H5IO6-transformed cells, although an increased rate of DNA synthesis per se is not an indispensable precondition for IL-2 synthesis.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
D007376:Interleukin-2; D037102:Lectins; D010504:Periodic Acid; D009439:Neuraminidase