title: Localization of the replication control region on the physical map of the octopine Ti plasmid.
abstract: A series of small plasmids has been derived from the octopine plasmid pTi-B6 by in vitro manipulation. The smallest plasmid that is able to replicate in Agrobacterium tumefaciens contains the ampicillin-resistance determinant from Tn1, coupled to a piece of DNA that is homologous to HpaI fragment number 11 of the octopine Ti plasmid pTi-Ach 5. The incompatibility functions are also specified by this region.
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000235:genetics; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D009710:Nucleotide Mapping; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: C005394:octopine; D001120:Arginine; D004262:DNA Restriction Enzymes