Morphine treatment increases clonidine binding in brain cell cultures.
Exposure of murine brain cells in culture to 75 microM morphine for 6 days produced an increase in the number of [3H]clonidine binding sites without affecting the apparent affinity. Similar treatment increased the binding of this alpha 2-adrenergic receptor agonist to cell cultures prepared from cerebral cortex but not to cultures of brain minus cortex or to neuroblastoma-glioma hybrid cells which possess both opiate and alpha 2-adrenergic receptors.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D003000:Clonidine / Q000378:metabolism*; D005910:Glioma; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009020:Morphine / Q000494:pharmacology*; D009447:Neuroblastoma; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D011942:Receptors, Adrenergic, alpha; D009020:Morphine; D003000:Clonidine