Electron microscopic study of the polymyxin treated goat erythrocytes.
Polymyxin B produced dose dependent changes in the surface topography of the goat erythrocyte cells. Transformation from the normal biconcave discs through crenated structures to the final rounded or spherical shape was recorded by scanning electron microscopy. A maximum of three to four crenations per cell was recorded corresponding to a polymyxin dose of 15.62 micrograms/ml. Transmission electron microscopy of the ultrathin sections of treated or untreated erythrocytes indicated that the crenations were formed by protrusions of the plasma membrane, occurring presumably because of the local increase of membrane fluidity after polymyxin treatment. Changes in the shape of the erythrocytes to the ultimate rounded forms were also indicated by the transmission electron microscopy.
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure*; D006041:Goats; D006461:Hemolysis / Q000187:drug effects*; D007700:Kinetics; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011112:Polymyxin B / Q000494:pharmacology*; D011113:Polymyxins / Q000494:pharmacology*
D011113:Polymyxins; D011112:Polymyxin B