Human hybridomas from malignant gliomas.
Intratumoral lymphocytes from 12 patients undergoing craniotomy for malignant glioma were fused with a specially derived human myeloma line LICR-LON-HMy2. 71 stable hybridomas were obtained from 5 of the 12 patients. Such hybridomas had a DNA content equal to the sum of that present in lymphocytes and the parent myeloma. The hybridoma supernatants contained human monoclonal immunoglobulins. Glioma-binding activity was detected in 7 out of the 71 supernatants. These results suggest that malignant gliomas contain a population of B lymphocytes which may be involved in host defence against the tumour.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001932:Brain Neoplasms / Q000276:immunology*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005910:Glioma / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm