Purification and properties of succinic oxidase factor, an extracellular product of Staphylococcus aureus.
Mitochondrial respiration can be inhibited in vitro by a complex of exotoxins elaborated by Staphylococcus aureus. This complex, designated Succinic Oxidase Factor, consists of two components, one of which (A) has a molecular weight of 75,000, is heat-stable, and interferes with cytochrome c oxidase and the other (B) has a molecular weight of 42,000, is heat-labile, and interferes with succinate-cytochrome c reductase. Each component can be separated by Sephadex G200 chromatography and purified by isoelectric focussing. Component A focussed at pH 3.4 and component B focussed at pH 4.5. Antibody prepared in rabbits to Succinic Oxidase Factor could neutralise the biological activities of each component separately or the whole complex and could react serologically with each component in an agar precipitation test.
D000818:Animals; D002850:Chromatography, Gel; D003579:Cytochrome Reductases / Q000037:antagonists & inhibitors*; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D004591:Electrophoresis, Polyacrylamide Gel; D005098:Exotoxins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors*; D013211:Staphylococcus aureus / Q000032:analysis*; D014451:Ubiquinone / Q000494:pharmacology
D005098:Exotoxins; D014451:Ubiquinone; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV