[The function of glutamate receptors in the membrane vesicules, proteoliposomes and hybrid cells of the neuroblastoma N18Tg2a].
The transport and selective functions of the glutamate-binding proteins of the rat brain cortex synaptic membranes, were studied. The data on kinetics of absorption of the ions 22Na+, 86Rb+ and 45Ca++ by the membrane vesicles and liposomes containing receptor proteins, are presented. The specific features of the c. n. s. glutamate receptors functioning in the hybrid cells of the neuroblastoma N18Tg2a, are revealed. A selective activation of transport of the 22Na+ ions was found in this kind of model systems in presence of physiological concentrations of L-glutamate. The modelling of the glutamate receptors function depended on composition of lipids, presence of the endogenous peptide agent inhibiting binding of the H3-L-glutamate, and on the degree of the neuroblastoma differentiation. Monoclonal antibodies obtained for the receptor's recognizing areas blocked the functions of the glutamate-binding proteins in all the model systems.
D000818:Animals; D001692:Biological Transport; D001932:Brain Neoplasms / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000503:physiopathology*; D005971:Glutamates / Q000502:physiology*; D006822:Hybrid Cells / Q000502:physiology; D007700:Kinetics; D008081:Liposomes / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000502:physiology; D009447:Neuroblastoma / Q000503:physiopathology*; D011485:Protein Binding; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D013570:Synaptic Membranes / Q000502:physiology
D005971:Glutamates; D008081:Liposomes; D008565:Membrane Proteins; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter
Funktsii glutamatnykh retseptorov v membrannykh vezikulakh, proteoliposomakh i gibridnykh kletkakh neĭroblastomy N18Tg2a.