Somatostatin-detergent interaction.
The effect of cationic, anionic and nonionic detergents on the EPR spectrum of spin-labeled somatostatin has been studied. At detergent concentrations well above the critical micelle concentration, nonionic detergents do not alter the EPR spectrum. Sodium dodecyl sulfate markedly alters both the line height ratio and the hyperfine splitting constant, whilst dodecyltrimethylammonium bromide alters only slightly the hyperfine splitting constant and line height ratio. The somatostatin-sodium dodecyl sulfate complex appeared monodisperse by sedimentation equilibrium with about 17 g bound detergent per g peptide. Circular dichroic and difference spectra of the dodecyl sulfate-somatostatin complex show that the tryptophanyl residue is buried in a nonpolar environment and that the secondary and tertiary structure of the peptide is markedly altered. Sedimentation equilibrium studies suggest that two types of dodecyltrimethylammonium-somatostatin complex exist. One type resembles the dodecyl sulfate-peptide complex, whilst the other appears to include several peptide units with only about one gram bound detergent per gram peptide.
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D003902:Detergents / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D008823:Micelles; D008954:Models, Biological; D011092:Polyethylene Glycols / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013004:Somatostatin / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D000596:Amino Acids; D003902:Detergents; D008823:Micelles; D000644:Quaternary Ammonium Compounds; C017474:Lubrol WX; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D013004:Somatostatin; C022806:dodecyltrimethylammonium