A highly efficient method of cell infection with encephalomyocarditis virus RNA.
A method for infecting Krebs-II cells with encephalomyocarditis virus RNA based on treatment of the cells with DEAE-dextran at high concentration was elaborated. The specific infectivity of virion RNA was 3 X 10(8) plaque-forming units per microgram, i. e. comparable to the specific infectivity of the whole virus. A high proportion of the cell population could be infected by this method.
D002460:Cell Line; D003637:DEAE-Dextran / Q000494:pharmacology; D004686:Encephalomyocarditis virus / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000502:physiology*; D014162:Transfection; D010948:Viral Plaque Assay
D012367:RNA, Viral; D003637:DEAE-Dextran