Changes in [3H]inositol-labelled phosphoinositides of pig platelets in response to thrombin.
Pig platelet phosphoinositides have been labelled with [3H]inositol and then treated with thrombin in the absence of Ca2+. There was a loss of labelled phosphatidylinositol 4,5-bisphosphate between 30 and 60 s after the addition of thrombin but the general picture was of increased labelling over a 4-min period. Labelling of phosphatidylinositol 4-phosphate showed no period of loss but there was an early loss of phosphatidylinositol and no increased labelling during the 4-min incubation. The small amount of lysophosphatidyl[3H]inositol in the platelets was not affected by thrombin treatment. Thrombin caused loss of [14C]arachidonate-labelled phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D012995:Solubility; D013552:Swine; D013917:Thrombin / Q000494:pharmacology*
D001095:Arachidonic Acids; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D016718:Arachidonic Acid; D007294:Inositol; D013917:Thrombin