Two-dimensional electron transfer from cytochrome C to photosynthetic reaction centers.
The arrangement and the electron transfer are studied for photosynthetic reaction centers (RC) of Rhodopseudomonas sphaeroides reconstituted into phospholipid vesicles. Freeze-etch electron micrographs of phase separated mixed vesicles reveal an RC enrichment in the phase containing the acidic lipid serine. It is demonstrated that the electron transfer from cytochrome c to RC involves a two-dimensional diffusion of the membrane bound electron donor with diffusion coefficients (D approximately 10(-9) cm2/sec) characteristic for membrane proteins.
D001426:Bacterial Proteins / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine; D004579:Electron Transport; D005613:Freeze Etching; D007700:Kinetics; D008081:Liposomes; D008854:Microscopy, Electron; D008954:Models, Biological; D045322:Photosynthetic Reaction Center Complex Proteins; D012242:Rhodobacter sphaeroides / Q000378:metabolism* / Q000648:ultrastructure; D012694:Serine
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008081:Liposomes; D045322:Photosynthetic Reaction Center Complex Proteins; D012694:Serine; D004134:Dimyristoylphosphatidylcholine