Supernatant virus release as a differentiating marker between low passage and vaccine strains of human cytomegalovirus.
The ratio of CMV virus released into the supernatant per infected human fibroblast was studied for three laboratory strains and seven fresh isolates. The former all gave ratios over one whereas the latter gave ratios less than one. This phenomenon may serve as a marker of high passage attenuated virus, as about 25 passages were required in case of one strain to change this property.
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development* / Q000472:pathogenicity; D006801:Humans; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D014776:Virus Cultivation
D014613:Vaccines, Attenuated; D014765:Viral Vaccines