title: Transposon Tn1 intra-molecular transposition.
abstract: A system for the direct selection of intra- and inter-molecular transposition events has been used to show that intra-molecular transposition of Tn1 generates deletions and inversions and requires the tnpA but not the tnpR gene product, as predicted by current models of transposition. Intra-molecular Tn1 transposition is much less limited by 'transposition immunity' than inter-molecular transposition, and occurs at frequencies comparable to those for inter-molecular transposition. The selection system, which uses the bacteriophage lambda cI- PR region as a target can be used to select, quantify, and characterize any spontaneous or induced mutations.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D004251:DNA Transposable Elements