title: A simple perfusion chamber for studying neurotransmitter release from cells maintained in monolayer culture.
abstract: A perfusion chamber is described for studying the efflux of putative neurotransmitters from CNS cells maintained in monolayer culture. We have used this apparatus to investigate the efflux of newly accumulated [3H]GABA from cell cultures of the early postnatal rat cerebellum.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid