The structure determination of rabbit phosphoglucomutase.
Tetragonal crystals of rabbit phosphoglucomutase have been grown from solutions containing ammonium sulphate, polyethylene glycol solution and enzyme. There are two molecules, each of relative molecular mass 64 000 per asymmetric unit. A rotation function suggests that these are related by a twofold axis. X-ray diffraction data for five heavy-atom derivatives and native crystals have been collected by using oscillation photography. A tentative and partial solution of the KAu(CN)2 sites has been obtained. The enzyme in the native crystals is phosphorylated, but the phosphate can be removed without harm to the crystals. Similarly the essential Mg2+ ion can be removed or replaced by Zn2+. The enzyme is active in the native crystals.
D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D003460:Crystallization; D008274:Magnesium; D010733:Phosphoglucomutase / Q000378:metabolism; D011817:Rabbits; D014961:X-Ray Diffraction
D010733:Phosphoglucomutase; D008274:Magnesium