Mouse osteoblasts synthesize collagenase in response to bone resorbing agents.
Bone cells isolated from mouse calvariae by a sequential digestion procedure have many osteoblast characteristics: they respond to PTH and prostaglandin E2 by activation of adenylate cyclase but not to calcitonin, they stain for alkaline phosphatase and they make only type I collagen. In confluent monolayer culture, they do not secrete collagenase in appreciable quantities, unless stimulated with resorptive substances such as PTH, prostaglandin E2, 1,25(OH)2 vitamin D-3 and monocyte-conditioned medium. This suggests they play a direct role in bone resorption.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000187:drug effects; D002117:Calcitriol / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis; D015232:Dinoprostone; D004789:Enzyme Activation; D051379:Mice; D003012:Microbial Collagenase / Q000096:biosynthesis*; D010006:Osteoblasts / Q000201:enzymology*; D010281:Parathyroid Hormone / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology
D010281:Parathyroid Hormone; D011458:Prostaglandins E; D003094:Collagen; D000469:Alkaline Phosphatase; D003012:Microbial Collagenase; D000262:Adenylyl Cyclases; D002117:Calcitriol; D015232:Dinoprostone