Location of [125I]-angiotensin II receptors on rat kidney cortex epithelial cells.
Angiotensin II (AII) stimulates active Na+ extrusion from Na+ loaded renal cortex slices. Specific high affinity [125I]-AII binding sites in partially purified basolateral and brush-border epithelial membranes exhibit a KD of 0.88 nM and Bmax of 321.13 fmol mg-1 protein. Separation and purification of brush-border membranes yielded high affinity [125I]-AII binding sites with KD of 1.02 nM and Bmax of 56.6 fmol mg-1 protein. Angiotensin II receptors of the same affinity are present on renal cortex brush-border and basolateral membranes but a greater proportion are located on the latter. These receptors may be involved in the direct control of Na+ and water transport by AII.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D004848:Epithelium / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008566:Membranes / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D007457:Iodine Radioisotopes; D011506:Proteins; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D000804:Angiotensin II; D000469:Alkaline Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase