Restriction cleavage maps of the DNAs of Streptococcus pneumoniae bacteriophages containing protein covalently bound to their 5' ends.
Several pneumococcal bacteriophages showing a morphology similar to that previously described for Cp-1 (Ronda et al. 1981) have been isolated and purified from throat samples taken from healthy children. Three of these phages (Cp-5, Cp-7 and Cp-9) have been studied in detail and compared to Cp-1. The four phages differed in several respects, e.g. size, structural polypeptides, restriction enzyme cleavage patterns, etc. The DNA of Cp-5, Cp-7 and Cp-9 showed protease-sensitive transfecting activity. This, together with the results obtained by electrophoretic analyses as well as by isotopic labelling of these DNAs with [gamma-32P]ATP and polynucleotide kinase indicated that all these new phages have a protein covalently linked to the 5' ends of their DNAs as in the case of Cp-1 (García et al. 1983). Restriction enzyme cleavage maps of Cp-1, Cp-5, Cp-7 and Cp-9 have been constructed.
D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D003852:Deoxyribonucleoproteins / Q000502:physiology*; D013296:Streptococcus pneumoniae; D014162:Transfection; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes