Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.
F8dl is a simian virus 40 early-region deletion mutant that lacks the simian virus 40 DNA sequences between 0.168 and 0.424 map units. Despite this large deletion, cloned F8dl DNA transforms Fisher rat F111 cells and BALB/3T3 clone A31 mouse cells as efficiently as does cloned simian virus 40 wild-type DNA. These results indicate that less than 40% of the large T-antigen-coding sequence is required for efficient transformation.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002872:Chromosome Deletion; D005796:Genes; D005814:Genes, Viral; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D051381:Rats; D011916:Rats, Inbred F344; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000951:Antigens, Neoplasm; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins