title: Transient change of organization of vimentin filaments during mitosis as demonstrated by a monoclonal antibody.
abstract: A monoclonal antibody specific for vimentin is described which, by immunofluorescence and immunoelectron microscopy, decorates fibrillar and/or granular structures in mitotic and early postmitotic cells but does not react with vimentin filaments of interphase stages of various cultured cells (rat vascular smooth muscle-derived cell line RVF-SM; SV40-transformed human fibroblasts; bovine kidney epithelial cells of line MDBK). These observations indicate that the organization of vimentin filaments varies during the cell cycle, undergoing a perimitotic change of filament organization. These changes of vimentin filaments are described in relation to those reported for cytokeratin filaments of various epithelial and carcinoma cells. The possible functional implications of filament protein rearrangements both during the cell cycle and in cell differentiation processes are discussed.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000166:cytology* / Q000648:ultrastructure; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron; D008938:Mitosis; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D051381:Rats; D013539:Simian virus 40; D013997:Time Factors; D014746:Vimentin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014746:Vimentin