Protamine sulfate precipitation: a new assay for the Ah receptor.
The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.
D000818:Animals; D001667:Binding, Competitive; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D003600:Cytosol / Q000032:analysis; D017886:Durapatite; D006882:Hydroxyapatites; D008099:Liver / Q000032:analysis*; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism; D011479:Protamines; D051381:Rats; D011919:Rats, Inbred Strains; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000032:analysis*
D006882:Hydroxyapatites; D000072317:Polychlorinated Dibenzodioxins; D011479:Protamines; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; D017886:Durapatite