title: Cryptic plasmids in hospital isolates of Providencia stuarti.
abstract: The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic plasmids was carried by 40 isolates, the remainder were plasmid-free. A higher proportion of one cryptic plasmid (CPT-A) was found in environmental isolates than in isolates from patients. The serotype of all isolates of P. stuarti was O63 and they were epidemiologically related. Two of the eight patients colonised by P. stuarti carried all three possible variants: plasmid-free (PFI) strains or strains containing cryptic plasmid A (CPT-A) or cryptic plasmid B (CPT-B). The epidemiological significance of these results is discussed.
mesh_terms: D000368:Aged; D001437:Bacteriuria / Q000382:microbiology; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D005243:Feces / Q000382:microbiology; D006757:Hospital Units; D006801:Humans; D008297:Male; D010957:Plasmids; D011511:Proteus / Q000235:genetics*; D011532:Providencia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D012867:Skin / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases