Comparison of protein sulfation in control and virus-transformed baby hamster kidney cells.
Protein sulfation in baby hamster kidney cells (BHK) and their polyoma virus transformants (PY-BHK) was studied comparatively. On in vivo labeling, [35S]-sulfate was incorporated into the 50K protein and proteins in the 100-180K range, represented by the 155K protein. The incorporation into both the 50K and 155K protein was elevated 2-3 fold in PY-BHK cells compared to in BHK cells. Tyrosine-O-sulfate was the only identifiable sulfated amino acid in both proteins. On in vitro labeling with [35S]3'-phosphoadenosine 5'-phosphosulfate (PAPS), at least 6 radioactive protein bands were discernible on gel electrophoresis. Of these, sulfation of the 57K and 60K proteins was elevated in PY-BHK cells compared to in BHK cells, whereas sulfation of the 39K protein was depressed in PY-BHK cells. Tyrosine-O-sulfate was the only identifiable sulfated amino acid in these proteins.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D007668:Kidney / Q000378:metabolism*; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D011120:Polyomavirus / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D013463:Sulfuric Acid Esters / Q000378:metabolism*; D013464:Sulfuric Acids / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D011506:Proteins; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; C005662:tyrosine O-sulfate; D014443:Tyrosine; D010724:Phosphoadenosine Phosphosulfate