Broad host range cloning vectors for gram-negative bacteria.
A series of cloning vectors has been constructed based on the broad-host-range plasmid R300B. One of these vectors, pGSS33, has a size of 13.4 kb and carries four antibiotic resistance genes [ampicillin (Apr), chloramphenicol (Cmr), streptomycin (Smr) and tetracycline (Tcr)], all of which have restriction sites for insertional inactivation. The derivation, structure and uses of the plasmids are described.
D000900:Anti-Bacterial Agents / Q000633:toxicity; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D019605:Euryarchaeota / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D011815:R Factors
D000900:Anti-Bacterial Agents; D004262:DNA Restriction Enzymes