title: The mitogenic effect of the lymphocytosis promoting factor from Bordetella pertussis on human T gamma and non-T gamma cells.
abstract: We studied the effect of lymphocytosis promoting factor (LPF), derived from the supernatant fluid of a culture of phase I Bordetella pertussis strain Tohama, on human lymphocyte proliferation. LPF was a potent mitogen for human mononuclear cells, specifically T cells. LPF failed to induce cytoplasmic immunoglobulin production by B cells. Removal of the monocytes from the T cell fraction diminished responses to LPF, but the response could be restored completely by the addition of 5.0% monocytes. These results suggest that LPF-induced cell proliferation is at least partially dependent on monocytes. In contrast to PHA, LPF stimulated T gamma cells to a greater extent than non-T gamma cells, but the magnitude of the T gamma or non-T gamma cell response was less than that of T cells, indicating that synergistic interactions between T gamma and non-T gamma cells are required for maximal response.
mesh_terms: D001427:Bacterial Toxins / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000276:immunology*; D002450:Cell Communication; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000008:administration & dosage*; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation; D008938:Mitosis; D009000:Monocytes / Q000276:immunology; D037342:Pertussis Toxin; D013601:T-Lymphocytes / Q000276:immunology*; D010566:Virulence Factors, Bordetella
chemical_list: D001427:Bacterial Toxins; D007376:Interleukin-2; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin