Use of reversed-phase high-performance liquid chromatography for simultaneous determination of glutamine synthetase and glutamic acid decarboxylase in crude extracts.
Glutamine and gamma-aminobutyric acid (GABA), formed from glutamic acid in crude tissue extracts by glutamine synthetase and glutamic acid decarboxylase respectively, were separated by derivatization with dansyl chloride followed by reversed-phase high-performance liquid chromatography on the Altex Ultrasphere ODS-5 column. The mobile phase was a gradient of 100 mM potassium dihydrogen phosphate (pH 2.1) with 0-40% acetonitrile. The amounts of glutamine and GABA formed from glutamic acid were determined under different reaction conditions.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005974:Glutamate-Ammonia Ligase / Q000032:analysis*; D012160:Retina / Q000201:enzymology*
D005974:Glutamate-Ammonia Ligase