EPR studies on the photosystem II donor side in salt-washed and reconstituted inside-out thylakoids.
EPR measurements on inside-out thylakoids revealed that salt-washing, known to inhibit oxygen evolution and release a 23 and a 16 kDa protein, induced a Signal IIf and decreased the EPR signal from state S2. Readdition of the released 23 kDa protein restored the oxygen evolution and decreased the Signal IIf, but did not relieve the decrease in the state S2 signal. It is suggested that salt-washing inhibits the electron transfer from the oxygen-evolving site to Z, the physiological donor to P680. In inhibited photosystem II units lacking Signal IIf, Z+ is rapidly reduced, possibly by a modified S-cycle unable to evolve oxygen.
D003624:Darkness; D004578:Electron Spin Resonance Spectroscopy; D009940:Organoids / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D010944:Plants / Q000378:metabolism*; D012492:Salts; D013816:Thermodynamics
D012492:Salts; D010100:Oxygen