title: Leukotriene formation by bovine polymorphonuclear leukocytes.
abstract: The leukotriene production by bovine polymorphonuclear leukocytes isolated from peripheral blood has been studied. Cells were incubated in the presence of arachidonic acid, glutathione, calcium ionophore A23187 and Ca2+. The leukotrienes then formed are leukotriene C4, leukotriene B4, two all-trans isomers of leukotriene B4 and the double dioxygenation product 12-epi-6-trans-8-cis-leukotriene B4. Leukotriene C4 is formed in such a large quantity by the bovine polymorphonuclear leukocyte that it might constitute an excellent and inexpensive source for the biosynthetic preparation of this spasmogenic leukotriene.
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D007700:Kinetics; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000097:blood; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013189:SRS-A / Q000096:biosynthesis* / Q000097:blood
chemical_list: D013189:SRS-A; D007975:Leukotriene B4; D000001:Calcimycin; D002118:Calcium