The production of arachidonate lipoxygenase products by rat intestine is increased by phenolphthalein.
Rat isolated intestine incubated in Krebs solution converted exogenous [14C]arachidonic acid into products that chromatographed with prostaglandins, leukotriene B4 and 5-hydroxy-eicosatetraenoic acid. The accumulation of these products was increased by phenolphthalein 3.14-1570 microM and reduced by indomethacin 2.8 microM. This raises the possibility that laxation by phenolphthalein involves the formation of arachidonate lipoxygenase and cyclo-oxygenase products.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D002400:Cathartics / Q000494:pharmacology*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D007422:Intestines / Q000187:drug effects* / Q000201:enzymology; D007975:Leukotriene B4 / Q000096:biosynthesis; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D020113:Phenolphthalein; D010635:Phenolphthaleins / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D002400:Cathartics; D006893:Hydroxyeicosatetraenoic Acids; D010635:Phenolphthaleins; D011453:Prostaglandins; D007975:Leukotriene B4; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D020113:Phenolphthalein; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase