Reduction of cytochrome b-561 through the antimycin-sensitive site of the ubiquinol-cytochrome c2 oxidoreductase complex of Rhodopseudomonas sphaeroides.
Cytochrome b-561 of the ubiquinol-cytochrome c2 oxidoreductase complex of Rhodopseudomonas sphaeroides is reduced after flash illumination in the presence of myxothiazol in an antimycin-sensitive reaction. Flash-induced reduction was observed over the redox range in which cytochrome b-561 and the Q-pool are both oxidized before the flash. The extent of reduction increased with increasing pH, and was maximal at pH greater than 10.0 where the extent approached that observed in the presence of antimycin following a group of flashes. Reduction of cytochrome b-561 in the presence of myxothiazol showed a lag of approximately 1 ms after the flash, followed by reduction with t 1/2 approximately 6 ms; by analogy with the similar kinetics of the quinol oxidase site, we suggest that the rate is determined by collision with the QH2 produced in the pool on flash excitation.
D000968:Antimycin A / Q000494:pharmacology*; D020130:Bacterial Chromatophores / Q000201:enzymology; D003573:Cytochrome b Group / Q000378:metabolism*; D014450:Electron Transport Complex III; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008689:Methacrylates; D009097:Multienzyme Complexes / Q000378:metabolism* / Q000528:radiation effects; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000378:metabolism* / Q000528:radiation effects; D012242:Rhodobacter sphaeroides / Q000201:enzymology*; D013053:Spectrophotometry; D013104:Spheroplasts / Q000201:enzymology; D013844:Thiazoles / Q000494:pharmacology
D003573:Cytochrome b Group; D008689:Methacrylates; D009097:Multienzyme Complexes; D013844:Thiazoles; C023646:cytochrome b561; D000968:Antimycin A; C030517:myxothiazol; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; D014450:Electron Transport Complex III