Restoration of viability to an Escherichia coli mutant deficient in the 5'----3' exonuclease of DNA polymerase I.
An Escherichia coli polA (Ex) mutant that is usually inviable at restrictive temperatures (43 degrees C) was found to grow normally at 43 degrees C when incubated in the presence of a membrane-containing fraction prepared from E. coli. This membrane fraction causes anaerobic conditions that are necessary but not sufficient for restoration of viability since some component present in the membrane fraction is also required for colony formation at 43 degrees C. The accumulation of small DNA fragments typical of aerobic growth of the polA(Ex) mutant was also seen under anaerobic conditions. The polA(Ex) strain was also much more sensitive than the isogenic wild-type strain to hydrogen peroxide.
D000693:Anaerobiosis; D002872:Chromosome Deletion; D004256:DNA Polymerase I / Q000235:genetics*; D004261:DNA Replication; D004351:Drug Resistance; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005092:Exonucleases / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D012965:Sodium Chloride; D011189:Potassium Chloride; D004256:DNA Polymerase I; D005092:Exonucleases