Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.
Three mutations that alter the DNA sequence specificity of the catabolite gene activator protein (CAP) from AA-TGTGA--T---TCA-ATW to AA-TGTAA--T---TCA-ATW have been isolated. All three mutations affect the same amino acid of CAP, glutamic acid 181. We propose that it is this amino acid of CAP that makes contacts with base pairs 7 and 16 of the symmetrical recognition site.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D000242:Cyclic AMP / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D007763:Lac Operon; D009154:Mutation; D011953:Receptors, Cyclic AMP / Q000235:genetics* / Q000378:metabolism; D013329:Structure-Activity Relationship
D004268:DNA-Binding Proteins; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP