title: Recovery of viral agents from the central nervous system of cats.
abstract: Isolation of viruses from the central nervous system (CNS) of cats was attempted using an explant culture technique and subsequent co-cultivation with Crandell feline kidney (CRFK) or Vero cells. Feline syncytia-forming virus was isolated from the CNS of 11 of 16 cats where the initial co-cultivation was with CRFK cells. Feline panleucopaenia virus was isolated from the CNS of 2 adult cats. Co-cultured cells from the CNS of 3 cats contained eosinophilic cytoplasmic and intranuclear inclusions. The cytoplasmic inclusions consisted of tubular structures, 16-18 nm in diameter and up to 500 nm in length, which were similar in morphology to paramyxovirus nucleocapsids. The 3 co-cultured cells with cytoplasmic and intranuclear inclusions showed haemadsorption of guinea pig erythrocytes. The possible identity of these structures, and their association with a previously described primary focal demyelinating lesion in the CNS of cats, is discussed.
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology*; D002213:Capsid / Q000648:ultrastructure; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats / Q000382:microbiology*; D002478:Cells, Cultured; D003711:Demyelinating Diseases / Q000382:microbiology / Q000662:veterinary; D028301:Feline Panleukopenia Virus / Q000302:isolation & purification; D006168:Guinea Pigs; D006382:Hemadsorption; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D007668:Kidney; D009900:Optic Nerve / Q000382:microbiology*; D012190:Retroviridae / Q000302:isolation & purification; D014776:Virus Cultivation; D014780:Viruses / Q000302:isolation & purification* / Q000648:ultrastructure