Immune reactivity against membranes containing ganglioside GM1 in chronic-progressive multiple sclerosis: observation by spin-membrane immunoassay.
A spin-membrane immunoassay has been employed to examine the immune reactivity of whole serum from patients with chronic-progressive multiple sclerosis (CPMS) against liposomes containing ganglioside GM1. Exposure to serum resulted in complement-mediated lysis of the GM1-liposomes. No lysis occurred with liposomes devoid of ganglioside. The mean (+/- S.E.M.) lysis values were 52.6 (+/- 9.8)% for fifteen CPMS patients and 32.9 (+/- 7.2)% for nine controls. The difference between the means was highly significant (student's t-test, P less than 0.0001), indicating increased anti-ganglioside immunity in patients with CPMS.
D003602:Cytotoxicity, Immunologic; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D005677:G(M1) Ganglioside / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008081:Liposomes / Q000276:immunology; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D013113:Spin Labels
D005732:Gangliosides; D008081:Liposomes; D013113:Spin Labels; D005677:G(M1) Ganglioside