Biosynthesis of somatostatin in pancreatic islets of Wistar rats and its regulation by glucose.
Pancreatic islets of Wistar rats incorporate L-[3H]-phenylalanine into proteins which react after gentle trypsination with a specific somatostatin antiserum. These somatostatin-like peptides were separated by gel chromatography and found to be a 3.5 kilodalton peptide, a peptide co-eluating with somatostatin (1.7 kd) and a low molecular weight peptide (1.0 kd). The biosynthesis of somatostatin may be regulated by glucose. The highest incorporation rate was measured in islets isolated from fasted rats and incubated in low glucose concentration.
D000818:Animals; D005215:Fasting; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D013004:Somatostatin / Q000096:biosynthesis* / Q000302:isolation & purification
D013004:Somatostatin; D005947:Glucose