Physical characterization of the Clostridium perfringens tetracycline-chloramphenicol resistance plasmid pIP401.
Four restriction endonucleases were used to construct a physical map of the tetracycline-chloramphenicol resistance plasmid pIP401, which had been isolated from Clostridium perfringens. Twenty-seven restriction sites were placed within the 52-kb map, including 1 site for AvaI, 2 sites for KpnI, 10 sites for EcoRI and 14 sites for PstI. The loss of chloramphenicol resistance in the derived pIP406 plasmid was associated with a deletion of a 6.2-kb DNA segment located in a 10.55 EcoRI-PstI fragment. Two 1.4-kb inverted repeats were also characterized in both pIP401 and pIP406.
D002701:Chloramphenicol / Q000494:pharmacology*; D003016:Clostridium perfringens / Q000187:drug effects / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D011815:R Factors; D012091:Repetitive Sequences, Nucleic Acid; D013752:Tetracycline / Q000494:pharmacology*
D004269:DNA, Bacterial; D002701:Chloramphenicol; D004262:DNA Restriction Enzymes; D013752:Tetracycline