[Use of different media for growing the producer of the restriction enzyme XBA I].
The possibility of using culture media prepared from local ingredients and intended for growing the producers of restricting enzyme Xba 1 has been demonstrated. The yield of restricting enzyme Xba 1 per g of crude biomass, obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories (USA), has proved to be 4 times greater than that obtained with the use of peptone-yeast medium prepared from local ingredients. At the same time the use of casein-saline medium ensures the yield of the enzyme, similar to that obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories, but with a greater content of nonspecific nucleases.
D003470:Culture Media / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D013997:Time Factors; D014974:Xanthomonas / Q000201:enzymology / Q000254:growth & development*
D003470:Culture Media; D004262:DNA Restriction Enzymes; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific
Ispol'zovanie razlichnykh sred dlia vyrashchivaniia produtsenta restriktiruiushchego fermenta XBA I.