[The role of lipolysis and lipid peroxidation in the induction of ion transport in mitochondria after freezing and thawing].
The reasons of ion transport induction observed after freezing-thawing of the rat liver mitochondria were investigated. It is found that a fall in the membrane potential value, an increase in the respiration rate and K+ ion release from mitochondria to the incubation medium with phosphate are averted when succinate is replaced by the Kreb's cycle substrates and when ionol (an antioxidant) or nupercain (an inhibitor of mitochondrial phospholipase A2) are incorporated into the incubation medium. The induction of ion transport is caused by the activation of peroxide processes and of lipolysis associated with them. It is supposed that under experimental conditions after freezing-thawing a degree of pyridine nucleotides and glutathione reduction lowers, that in its turn leads to the inhibition of the glutathione-peroxidase activity and development of peroxide processes.
D000818:Animals; D001692:Biological Transport; D005615:Freezing; D007425:Intracellular Membranes / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008066:Lipolysis; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014021:Tissue Preservation
D007473:Ion Channels; D008054:Lipid Peroxides
Rol' lipoliza i perekisnogo okisleniia lipidov v induktsii ionnogo transporta mitokhondriĭ posle zamorazhivaniia-ottaivaniia.