title: Anisomycin blocks the late phase of long-term potentiation in the dentate gyrus of freely moving rats.
abstract: Freely moving rats, chronically implanted with stimulation electrodes in the medial entorhinal cortex and recording electrodes in the dentate gyrus, received two 400 micrograms intraventricular injections of anisomycin during a tetanization procedure that induced a long-lasting potentiation (72 hours) of the monosynaptic field potential. Inhibition of protein synthesis during the tetanization procedure did not immediately influence the induction of long-term potentiation (LTP). However, 3-4 hours after the beginning of tetanization the potentiation effect decayed progressively and was abolished totally during the remaining 7 day observation period. In control experiments anisomycin did not affect the slope of field EPSP's and produced a reversible depression of the population spike amplitude. These data indicate a relatively specific effect of the protein synthesis inhibitor on mechanisms involved in a late phase of LTP stabilization.
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D003864:Depression, Chemical; D005071:Evoked Potentials / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009419:Nerve Tissue Proteins / Q000502:physiology; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D009419:Nerve Tissue Proteins; D011759:Pyrrolidines; D000841:Anisomycin; D002118:Calcium