Preservation of gastric gland character in transplantable gastric adenocarcinoma (SG2B) of rats.
The morphological and biochemical characteristics of a transplantable tumor line of rat gastric carcinoma (SG2B), that developed in a Wistar strain rat after treatment with N-methyl-N'-nitro-N-nitrosoguanidine and then 4-nitro-quinoline 1-oxide were examined at the 60th passage. The tumor was serially transplanted into newborn rats by subcutaneous and intraperitoneal routes and has been passed through 60 transplant generations over a 4-year period. The tumors appeared to be well-differentiated tubular adenocarcinomas throughout successive passages, and at each generation transplants were almost identical to the original gastric adenocarcinoma as judged by light and electron microscopic observation. Ultrastructurally, the neoplastic cell contained abundant free ribosomes and exhibited few mucin granules. The microville on the apical cell surface were short and irregularly spaced. Electrophoresis showed that pepsinogens (Pg) 3 and 4 were present but no Pg 1 was detectable at the 60th passage, as was the case in the tissue at the 2nd and 5th passages. The pepsinogen content of the tissue at the 60th passage was similar to that at the 5th passage. The tumor tissue has thus retained the morphological and biochemical character of a pyloric gland type tumor for a long time (over 4 years) during serial transplantation.
D015112:4-Nitroquinoline-1-oxide; D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D008297:Male; D008769:Methylnitronitrosoguanidine; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D010435:Pepsinogens / Q000378:metabolism; D051381:Rats; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000473:pathology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D010435:Pepsinogens; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D005723:gamma-Glutamyltransferase