Solubilization and purification of Artemia salina (Na,K)-activated ATPase and NH2-terminal amino acid sequence of its larger subunit.
Membrane bound (Na,K)-ATPase partially purified from the nauplius larva of the brine shrimp, Artemia salina, was solubilized with the non-ionic detergent C12E8 in the presence of KCl. The addition of KCl was essential for protecting the enzyme against inactivation. With solubilization the enzyme could then be purified to apparent homogeneity. Electron microscopic observation of the purified enzyme revealed a homogeneous population of particles with a diameter of approximately 4 nm. The larger (alpha) subunit of the enzyme formed double bands on sodium dodecyl sulfate-polyacrylamide gels. NH2-terminal sequence analysis of the alpha subunit revealed the possible presence of two isoforms of (Na,K)-ATPase. At the third position a small but distinct amount of lysine was found in addition to glycine, suggesting that the two forms are different from each other at least at the third residue. The NH2-terminal sequence determined is as follows. NH2-Ala-Lys-Gly (Lys)-Lys-Gln-Lys-Lys-Gly-Lys-Asp-Leu-Asn-Glu-Leu-Lys-Lys-Glu-Leu-Asp-Il e-Asp -Phe-His-Lys-Ile-Pro- The sequence is abundant in hydrophilic amino acids, especially lysine, and is quite different from those of vertebrate enzymes reported so far.
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D004285:Dogs; D004372:Ducks; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007814:Larva; D046911:Macromolecular Substances; D012491:Salt Gland / Q000201:enzymology; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D013045:Species Specificity
D046911:Macromolecular Substances; D000254:Sodium-Potassium-Exchanging ATPase