Quantitative receptor autoradiography: tissue defatting eliminates differential self-absorption of tritium radiation in gray and white matter of brain.
A four-fold greater absorption (quenching) of tritium emissions by white matter relative to gray matter produces a false 'contrast' effect in autoradiographs of 3H-ligand binding to brain sections. The differential absorption is eliminated by tissue defatting prior to autoradiographic exposure.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000378:metabolism*; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004745:Enkephalins / Q000378:metabolism; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D011422:Propionates / Q000378:metabolism; D051381:Rats; D011955:Receptors, Drug / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*; D017450:Receptors, Opioid, mu; D013388:Succinimides / Q000378:metabolism; D014316:Tritium
D004745:Enkephalins; D011422:Propionates; D011955:Receptors, Drug; D011957:Receptors, Opioid; D017450:Receptors, Opioid, mu; D013388:Succinimides; D014316:Tritium; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; C021749:N-succinimidyl propionate; C022581:Bolton-Hunter reagent