Phenacein--an angiotensin-converting enzyme inhibitor produced by a streptomycete. I. Taxonomy, fermentation and biological properties.
Phanacein, 3,6-dihydroxy-1-phenazinecarboxylic acid, was a specific angiotensin-converting enzyme (ACE) inhibitor isolated from a member of the Streptomyces tanashiensis-zaomyceticus group. Phenacein acted as a pure competitive inhibitor with a Ki of 0.58 microM. ACE inhibition could be reversed by Zn++, but not by Co++, Ca++, or Mg++; therefore, phenacein may chelate the active site zinc of ACE. However, other zinc-containing enzymes were not inhibited at high phenacein concentrations. Phenacein exhibited weak activity against Gram-positive bacteria, but was not active against Candida sp. or Gram-negative organisms.
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D005285:Fermentation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D010619:Phenazines / Q000494:pharmacology*; D011817:Rabbits; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D010619:Phenazines; C043271:phenacein; D015032:Zinc