Calcium compartments and fluxes are affected by the src gene product of Rat-1 cells transformed by temperature-sensitive Rous sarcoma virus.
Total cellular calcium content (determined by atomic absorption spectrometry) of Rat-1 cells transformed by temperature-sensitive Rous sarcoma virus decreases with cell density, but is found not significantly different at permissive and at non-permissive temperature. Kinetic analysis of 45Ca efflux from preloaded cells exhibits three separable pools of exchangeable calcium. The ratio of pool size of the fast-exchanging Ca-compartment (bound to cell surface) to pool size of the intermediate Ca-compartment (cytoplasmic) was found to decrease from 2.5 to 1.3 upon shift from non-permissive to permissive temperature. The slowly exchanging Ca-pool (presumably mitochondrial) did not change significantly upon temperature shift. These and further data demonstrate a close correlation between distribution of cellular Ca among different cellular compartments and characteristics of cellular proliferation, both attributable to the function(s) of a single oncogene.
D000818:Animals; D001358:Avian Sarcoma Viruses; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation; D002472:Cell Transformation, Viral; D007700:Kinetics; D051381:Rats; D011916:Rats, Inbred F344; D013054:Spectrophotometry, Atomic; D014764:Viral Proteins / Q000494:pharmacology*
D014764:Viral Proteins; D002118:Calcium