title: Lethal effect of a heterologous murein hydrolase on penicillin-treated Streptococcus sanguis.
abstract: Nine strains of Streptococcus sanguis exhibited tolerance to benzylpenicillin: the growth of each strain was susceptible to penicillin with minimal inhibitory concentrations of 0.1 mug/ml or lower, but the bacteriolytic and bactericidal effects were limited in each case. The tolerance of these bacteria was also reflected in the large discrepancies between the minimal inhibitory and minimal bactericidal concentrations for benzylpenicillin. The hypothesis that a natural deficiency of endogenous murein hydrolase (autolysin) in this species accounts for the penicillin tolerance was tested by using a heterologous murein hydrolase, the C-phage-associated lysin. In seven of the strains, addition of the lysin to the culture together with penicillin or other cell wall inhibitors resulted in lysis and rapid loss of viability. The enzyme alone did not appreciably affect normally growing cultures. The irreversible effects of penicillin plus lysin were drastically reduced in the presence of the bacteriostatic agents chloramphenicol and cerulenin. Speculations based on experiments are presented for the mechanisms by which penicillin treatment sensitizes these bacteria to an exogenous lytic enzyme. Similar phenomena requiring cooperation of host factors and penicillin may occur during infection, since somewhat similar although less pronounced results were obtained by addition of human lysozyme to penicillin-treated S. sanguis.
mesh_terms: D000581:Amidohydrolases / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism; D004347:Drug Interactions; D005019:Ethyl Ethers / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000494:pharmacology*; D010400:Penicillin G / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology*; D013298:Streptococcus sanguis / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005019:Ethyl Ethers; D010406:Penicillins; D014050:Toluene; D000581:Amidohydrolases; D009238:N-Acetylmuramoyl-L-alanine Amidase; D010400:Penicillin G