Prolactin receptors in tilapia (Sarotherodon mossambicus) tissues: binding studies using 125I-labeled ovine prolactin.
The binding of 125I-labeled ovine prolactin (oPRL) to membrane preparations of tissue from freshwater-adapted tilapia (Sarotherodon mossambicus) was examined. Liver, ovary, and testis showed a relatively high specific binding (5-10%). A lower specific binding occurred consistently in intestine and gill tissue, and inconsistently in urinary bladder and kidney preparations. Desaturation experiments with MgCl2 indicated that a majority of the PRL receptors were already occupied by endogenous PRL. Scatchard analysis of liver binding gave a dissociation constant of 0.6 X 10(-9) M and a capacity of 207 fmol/mg protein.
D000818:Animals; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000378:metabolism; D006066:Gonads / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011981:Receptors, Prolactin; D012756:Sheep; D001743:Urinary Bladder / Q000378:metabolism
D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011388:Prolactin