title: Studies of the influence of immunological and serological factors from patients with cholestasis due to alcoholic or viral hepatitis on biliary function in the rat.
abstract: Studies were undertaken to determine if cholestasis in alcoholic or viral hepatitis is related to immunologic hyperreactivity as suggested for cholestasis due to type-II drug-induced hepatitis, and evaluate possible mechanisms involved in lymphokine-induced cholestasis. Results indicate that a cholestatic factor exists in alcoholic and acute viral hepatitis. Supernatants of lymphocytes from patients with alcoholic hepatitis stimulated by an extract of alcoholic hyalin evoked a 28% +/- 7.3 SEM reduction in rat bile flow (P less than 0.03). Supernatants of lymphocytes from patients with acute viral hepatitis activated by liver-specific protein caused a reduction in rat bile flow of 24% +/- 5.9 SEM (P less than 0.03). A decrease in bile flow also occurred following injections of sera from patients with alcoholic or acute viral hepatitis. In contrast, injection of supernatants of non-stimulated lymphocytes or those from chronic active hepatitis or healthy subjects did not produce a significant change in bile flow. Supernatants of stimulated lymphocytes from tuberculin-sensitized guinea pigs caused a similar decrease in rat bile flow and reduced excretion of human secretory immunoglobulin A (IgA). Despite reductions in rat bile flow there were no alterations in liver morphology, liver plasma membrane Na-K-ATPase activity, microsomal cholesterol-7 alpha-hydroxylase activity or low-dose indocyanine green clearance during the period of observation.
mesh_terms: D000328:Adult; D000818:Animals; D001646:Bile / Q000378:metabolism*; D002779:Cholestasis / Q000209:etiology*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D006519:Hepatitis, Alcoholic / Q000150:complications* / Q000503:physiopathology; D006525:Hepatitis, Viral, Human / Q000150:complications* / Q000503:physiopathology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000502:physiology; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D007071:Immunoglobulin A, Secretory; D002790:Cholesterol 7-alpha-Hydroxylase; D000254:Sodium-Potassium-Exchanging ATPase