Encephalomyocarditis virus RNA synthesis in vitro is protein-primed.
A crude membrane-bound replication complex isolated from encephalomyocarditis virus-infected cells is able to initiate the synthesis of viral RNA. Both the formation of the primer, VPg-pU, and its utilization for the initiation of RNA chains take place in this system. A significant amount of the synthesized VPg-pU is found in the free form. The predominant product of the de novo initiation is represented by short phenol-soluble VPg oligonucleotide species, and only a small percentage of the latter appear to be elongated into longer RNA chains.
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D004686:Encephalomyocarditis virus / Q000235:genetics*; D005814:Genes, Viral; D007700:Kinetics; D051379:Mice; D010766:Phosphorylation; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000302:isolation & purification / Q000502:physiology*
D012367:RNA, Viral; D014758:Viral Core Proteins; D014764:Viral Proteins