Characterization of Epstein-Barr virus nuclear antigen(s) in different cell lines by radioimmunoelectrophoresis.
Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67,000 and 70,000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70,000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73,000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73,000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72,000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.
D000956:Antigens, Viral / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight
D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens