Quantitative modification of immunohaemolytic sensitivity of bovine erythrocytes.
Experimental work was carried out to study the effect of periodate and neuraminidase on the J-specific and non-J-specific immunohaemolysis of bovine erythrocytes. The method of quantitative immunohaemolysis was applied for the determination of immunohaemolytic sensitivity. Both the periodate and neuraminidase treatment, while increasing J-specific immunohaemolysis, resulted in non-J-specific haemolysis. Periodate treatment that followed a neuraminidase one, however, decreased the enhancement of J-specific immunohaemolysis and resulted in a significant increase of non-J-specific haemolysis. The maximum increase of non-J-specific haemolysis was found in erythrocytes highly sensitive to anti-J-serum.
D000818:Animals; D001789:Blood Group Antigens; D002417:Cattle / Q000097:blood*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000276:immunology; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D006461:Hemolysis / Q000187:drug effects; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D013696:Temperature
D001789:Blood Group Antigens; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase