title: Isolation and basic properties of temperature--sensitive mutants of Venezuelan equine encephalomyelitis virus.
abstract: Fifteen temperature-sensitive (ts) mutants were isolated from 4 Venezuelan equine encephalomyelitis virus clones by nitrous acid treatment or by growing the virus in the presence of 5-fluoruracil. Three of them were classified as RNA- mutants by their inability to synthesize RNA at nonpermissive temperature (1.5-3.3% in respect to the wild type). The remaining 11 mutants showed a slight decrease of RNA synthesis at the nonpermissive temperature (32-73+) and were referred to the RNA+ phenotype. One mutant possessed RNA +/- phenotype (18%). Five complementation groups were determined by complementation analysis of the mutants.
mesh_terms: D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects / Q000235:genetics*; D005472:Fluorouracil / Q000494:pharmacology; D005816:Genetic Complementation Test; D009153:Mutagens; D009154:Mutation; D009608:Nitrous Acid / Q000494:pharmacology; D010641:Phenotype; D012367:RNA, Viral / Q000235:genetics*; D013696:Temperature
chemical_list: D009153:Mutagens; D012367:RNA, Viral; D009608:Nitrous Acid; D005472:Fluorouracil