title: Nmel, a restriction endonuclease from Neisseria meningitidis.
abstract: A restriction endonuclease, Nmel, present in Neisseria meningitidis was partially purified by passing through a blue 2-cross linked agarose column; no contaminating nucleases remained detectable. This enzyme cleaved phage lambda, adenovirus type 2 and phi x 174 DNA but did not cleave SV40 DNA. It had an absolute requirement for Mg2+ for its activity and was inhibited by high concentrations of NaCl or MgCl2. Nmel activity was completely abolished after 1 h of incubation at 65 degrees C. S-adenosyl-L-methionine and ATP had no effect on its activity suggesting that Nmel is a type II restriction endonuclease enzyme. It is the first report of a restriction enzyme present in N. meningitidis.
mesh_terms: D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D004492:Edetic Acid / Q000494:pharmacology; D006358:Hot Temperature; D008274:Magnesium / Q000494:pharmacology; D009345:Neisseria meningitidis / Q000201:enzymology*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D012965:Sodium Chloride; D004492:Edetic Acid; D004262:DNA Restriction Enzymes; D008274:Magnesium