Aerobactin iron uptake sequences in plasmid ColV-K30 are flanked by inverted IS1-like elements and replication regions.
By using Southern blot hybridization procedures, we found that a specific sequence within a 16.3-kilobase HindIII restriction fragment of pColV-K30 was also present in at least three other pColV-K30 HindIII fragments. Restriction endonuclease mapping of these HindIII fragments indicated that two of these repeated sequences, identified as IS1-like, occur in reverse orientation adjacent to the ends of the aerobactin iron uptake region, also included in the 16.3-kilobase HindIII fragment. There are two distinct replication regions enclosing the IS1-flanked aerobactin genes. A pColV-K30 BamHI restriction endonuclease fragment, carrying one of these replication regions, showed homology with the F plasmid EcoRI fragment f5, which carries the F replication sequences.
D001425:Bacterial Outer Membrane Proteins; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids; D011971:Receptors, Immunologic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D011971:Receptors, Immunologic; C034506:aerobactin receptor; C031819:aerobactin; D007501:Iron; D004262:DNA Restriction Enzymes