Purification and partial characterization of filamentous haemagglutinin from Bordetella pertussis using monoclonal antibodies.
Two monoclonal antibodies against filamentous haemagglutinin (F-HA) from Bordetella have been produced. In immunoblotting of a SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of high ionic strength extracts of Bordetella pertussis, the two monoclonal antibodies both stained several high molecular weight bands. The two monoclonal antibodies were used for immuno-affinity column one-step purification of F-HA from high-ionic-strength extracts of Bordetella pertussis. In gradient SDS-PAGE, the eluted F-HA is present as a major double band with a molecular weight of approximately 240,000 daltons and several minor bands with molecular weights down to 90,000 daltons. The monoclonal antibodies were used in a monoclonal antibody catching enzyme linked immunosorbent assay (ELISA) for sensitive and specific detection of F-HA. This ELISA system proved valuable in optimizing the elution conditions for the monoclonal affinity columns.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006388:Hemagglutinins / Q000302:isolation & purification*; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006388:Hemagglutinins; D012967:Sodium Dodecyl Sulfate