title: Control of ammonium ion level for efficient nanaomycin production.
abstract: The addition of a small amount of NH4+ to a complex medium increased nanaomycin production by Streptomyces rosa subsp. notoensis OS-3966. The best NH4+ donor for nanaomycin production was NH4+-saturated natural zeolite, with which the maximum titer of nanaomycin E was 760 micrograms/ml, about four fold higher than the control titer. In contrast, lowering NH4+ levels by adding NH4+-trapping agents such as untreated natural zeolite reduced antibiotic production.
mesh_terms: D000538:Aluminum Silicates / Q000494:pharmacology; D000935:Antifungal Agents / Q000096:biosynthesis*; D009285:Naphthoquinones / Q000096:biosynthesis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*; D017641:Zeolites; D000095622:Benzoisochromanequinones
chemical_list: D000538:Aluminum Silicates; D000935:Antifungal Agents; D009285:Naphthoquinones; D000644:Quaternary Ammonium Compounds; D017641:Zeolites; C023430:nanaomycin E; D000095622:Benzoisochromanequinones