CFS oligoclonal bands in MS and other neurological diseases detected by agarose isoelectric focusing and electrophoresis.
Agarose gel isoelectric focusing (IEF) and electrophoresis (EF) were compared for detection of CSF oligoclonal bands in multiple sclerosis (MS) and other neurological diseases. The CSF IgG/albumin ratio, the IgG-Index and the IgG synthesis per day in the CNS were also considered. IEF was highly sensitive, revealing oligoclonal bands in 100% of CSF from 40 clinically definite MS, while EF had a sensitivity of 70%. In 90 patients with other neurological diseases, IEF revealed oligoclonal bands in 25.5%, EF in 11.1%. The IgG-index was the most sensitive of the quantitative parameters.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002524:Cerebellar Ataxia / Q000134:cerebrospinal fluid; D002648:Child; D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing; D008297:Male; D008590:Meningoencephalitis / Q000134:cerebrospinal fluid; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000276:immunology; D043025:Oligoclonal Bands; D010523:Peripheral Nervous System Diseases / Q000134:cerebrospinal fluid; D013344:Subacute Sclerosing Panencephalitis / Q000134:cerebrospinal fluid
D007136:Immunoglobulins; D043025:Oligoclonal Bands