Angiotensin I-converting enzyme in amniotic fluid.
Angiotensin I-converting enzyme (ACE) is present in human amniotic fluid. We characterized the enzyme by both its antigenic and enzymatic properties. Using a specific direct radioimmunoassay, ACE was quantified and characterized in each of the 19 samples tested. Mean level was 136 +/- 83 ng/ml. Amniotic ACE completely crossreacted, like that in plasma and kidney, with antibodies raised against the lung enzyme. ACE activity in amniotic fluid averaged 8.7 +/- 5.6 microU/ml using Hip-His-Leu as substrate and was significantly correlated with ACE antigen levels. ACE was not associated with the cells or the free intracellular organelles in amniotic fluid, and the enzyme was present in soluble form. Angiotensinase activity and high levels of kininase activity were found in amniotic fluid. Inhibition studies with captopril and anti-human ACE antibodies suggest that angiotensinases and kininases other than ACE were also present. Because renin, mostly in inactive form, and angiotensinogen were also found in these amniotic fluids, it appears that a complete, although not fully activated, renin angiotensin system is present in amniotic fluid and fetal membranes during pregnancy.
D000328:Adult; D000653:Amniotic Fluid / Q000032:analysis / Q000201:enzymology* / Q000276:immunology; D000803:Angiotensin I / Q000378:metabolism; D000806:Angiotensin-Converting Enzyme Inhibitors; D000808:Angiotensinogen / Q000032:analysis; D001920:Bradykinin / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007106:Immune Sera / Q000276:immunology; D066298:In Vitro Techniques; D007703:Peptidyl-Dipeptidase A / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D011863:Radioimmunoassay; D012083:Renin / Q000032:analysis
D000806:Angiotensin-Converting Enzyme Inhibitors; D007106:Immune Sera; D000808:Angiotensinogen; D000803:Angiotensin I; D002216:Captopril; D007703:Peptidyl-Dipeptidase A; D012083:Renin; D001920:Bradykinin