Characterization and culture of sinusoidal endothelium from normal rat liver: lipoprotein uptake and collagen phenotype.
Sinusoidal endothelial cells from adult rat liver have been isolated by centrifugal elutriation and been established in primary culture. Their identification has made use of a novel process of these cells, the sequestration of human acetoacetylated low-density lipoprotein. After its administration in vivo, labeled acetoacetylated low-density lipoprotein within the liver was associated solely with sinusoidal endothelial cells and, when labeled with the stable fluorescent compound 3,3'-dioctadecylindocarbocyanine, provided a means of identifying isolated cells by fluorescence microscopy. In endothelial cell cultures, 90% of the cells were fluorescent and exhibited fenestrae. The collagen phenotype of cultures was assessed by an immunofluorescent approach, which revealed cell-associated type IV collagen only; types I, III, and V were undetectable. In other studies, it was found that these cells lacked factor VIII-R antigen and Weibel-Palade bodies, adding to the evidence that they differ substantially from large-vessel and other capillary endothelia.
D000818:Animals; D002232:Carbocyanines; D002469:Cell Separation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004727:Endothelium / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D008074:Lipoproteins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D003012:Microbial Collagenase; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010641:Phenotype; D011402:Pronase; D051381:Rats; D011919:Rats, Inbred Strains
D002232:Carbocyanines; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D008074:Lipoproteins; D008077:Lipoproteins, LDL; C024286:3,3'-dioctadecylindocarbocyanine; D003094:Collagen; D011402:Pronase; D003012:Microbial Collagenase