Purification of a UTP:D-glucose-1-phosphate uridylyltransferase from Golgi apparatus of cat liver by affinity chromatography on UTP-agarose.
UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characteristics, UDP-glucose pyrophosphorylase from Golgi apparatus is different from cytosolic enzyme.
D000818:Animals; D002415:Cats; D002846:Chromatography, Affinity; D003902:Detergents; D006056:Golgi Apparatus / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D009713:Nucleotidyltransferases / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D012995:Solubility; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000302:isolation & purification*
C016178:5'-UTP-sepharose; D003902:Detergents; D012685:Sepharose; D009713:Nucleotidyltransferases; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase