NAD+ kinase: molecular weight determination by low-angle laser-light scattering.
Low-angle laser-light scattering (LALLS) was employed to measure the absolute molecular weight of chicken liver NAD+ kinase (NADK). The weight-average molecular weight (Mw) was found to be 275 000 +/- 15 000. The corresponding value for the second virial coefficient was -1.65 X 10(-3) ml X mol X g2. The value for Mw is in close accord with estimates reported for pigeon liver (270 000) and C. utilis (260 000) NADK. If the active enzyme is a dimer, the weight difference between pigeon/chicken liver and rabbit liver (136 000) NADK would indicate that the latter enzyme is an active monomer unit.
D000818:Animals; D001395:Azotobacter / Q000201:enzymology; D010856:Columbidae; D005260:Female; D005658:Fungi / Q000201:enzymology; D007834:Lasers; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D010063:Ovum / Q000201:enzymology; D010770:Phosphotransferases / Q000302:isolation & purification; D017853:Phosphotransferases (Alcohol Group Acceptor); D010944:Plants / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D012542:Scattering, Radiation; D012617:Sea Urchins; D013045:Species Specificity
D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase