title: Experiments in microbial evolution: new enzymes, new metabolic activities.
abstract: Biological evolution has resulted in a richness and diversity of species. Among microorganisms this is most evident in the wealth and diversity of biochemical transformations. Evidence for evolutionary relationships may be obtained from comparative studies, but with microorganisms it is also possible to follow evolution in action. Microbial populations adapt rapidly to changes in the environment and the evolution of new metabolic activities can be observed in laboratory experiments. The enzymes of many catabolic pathways are synthesized in response to the presence of inducing substrates. New catabolic activities may be acquired by mutations in regulatory genes resulting in alterations in the specificity of induction, or in enzyme synthesis in the absence of inducer. Mutations in structural genes may given rise to enzymes with altered substrate specificities. In bacteria, catabolic genes may be carried on plasmids and the exchange of plasmids among bacterial populations increases the evolutionary potential. Experiments in microbial evolution have produced strains with novel catabolic activities involving regulatory or structural gene mutations, gene duplications and plasmid exchange. Enzymes studied in this way include amidase, ribitol dehydrogenase, evolved beta-galactosidase, and enzymes of the catabolic pathways for pentoses and pentitols and haloaromatic compounds.
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase; D000581:Amidohydrolases