title: Identification, physical map location and sequence of the denV gene from bacteriophage T4.
abstract: The denV gene from bacteriophage T4, which codes for endonuclease V, a small DNA repair enzyme, has been cloned and identified by an approach combining DNA sequencing and genetics, independent of the phenotypic effect of the cloned gene. Appropriate DenV+ and DenV- deletion mutants were mapped physically to define precisely a region encompassing the denV gene. This region was sequenced in order to identify a protein-coding sequence of the correct size for the denV gene (400-500 bp). Finally, identification was confirmed by sequencing the corresponding fragments cloned from four genetically and phenotypically well-characterized denV mutants. The denV gene is located at 64 kb on the T4 genome, adjacent to the ipII gene, and codes for a basic protein of 138 amino acids with a deduced molecular weight of 16,078.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D013604:T-Phages / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004706:Endodeoxyribonucleases; D004262:DNA Restriction Enzymes; D043224:Deoxyribonuclease (Pyrimidine Dimer)