Cross-reaction of BK virus large T antigen with monoclonal antibodies directed against SV40 large T antigen.
Seventy-five percent of the amino acid sequence of simian virus 40 (SV40) large T antigen is identical to that of the large T antigen of the human papovavirus, BK virus (BKV). Cross-reactivity between BKV T antigen and monoclonal antibodies directed against SV40 T antigen was studied by immunofluorescence, an enzyme immunoassay, immunoprecipitation of radiolabeled extracts, and Western blotting. BKV T antigen was found to be recognized by two monoclonal antibodies, PAb 416 and 430, which react with two distinct sites toward the amino terminus of SV40 large T antigen. These two sites may correspond to two hydrophilic regions of shared amino acid sequence which exist toward the amino termini of the T antigens.
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D001739:BK Virus / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D011120:Polyomavirus / Q000276:immunology*; D013539:Simian virus 40 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor