[Interaction of n-alkanes with respiration and oxidative phosphorylation in rabbit heart mitochondria: n-hexane].
n-Hexane on coupled rabbit heart mitochondria induces "in vitro" uncoupling with both glutamate and succinate as substrates and the effect increases with increasing n-alkane concentration (from 0 to 160 microgram/mg mitochondrial protein) and temperature (from 15 degrees to 38 degrees C). The inner mitochondrial membrane is made permeable to proteins; moreover extrusion of some matrix enzymes and entry of exogenous NADH is produced. Furthermore at higher concentrations and temperatures NADH oxidase inhibition and increase of its thermosensitivity is shown whereas upon succinate oxidase is evidenced a biphasic effect (activation followed by inhibition). The results, qualitatively similar to those observed with detergents and solvents, suggest a fluidization of the lipid phase of the membrane.
D000818:Animals; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006586:Hexanes / Q000494:pharmacology*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D011817:Rabbits; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013696:Temperature
D005971:Glutamates; D006586:Hexanes; D009097:Multienzyme Complexes; D013386:Succinates; D009243:NAD; C026385:n-hexane; D018698:Glutamic Acid; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
Interazioni di n-alcani con la respirazione e fosforilazione ossidativa di mitocondri cardiaci di coniglio: n-esano.