Direct evidence for the expression of integrated hepatitis B virus DNA in human hepatoma tissue.
The intracellular structure and expression of integrated hepatitis B virus (HBV) DNA in the hepatoma tissue #1707 obtained from an 11-year-old boy were studied. It was found that most of the HBV genome was present in the #1707 DNA. Virus-specific 21S mRNA was also demonstrated by blot hybridization. This is the first direct evidence for the expression of the integrated viral genome in human hepatoma tissue.
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002648:Child; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis
D004279:DNA, Viral; D012333:RNA, Messenger