Cloning of Lac+ BamHI fragment into transposon Tn3 and transposition of the Tn3[lac] element.
By use of recombinant DNA techniques, we have inserted the lac+ operon into a transposon (Tn3). We constructed the recombinant in such a way that the essential step in assaying for transposition consisted of screening for bacteria with a thermostable Lac+ phenotype. Our results showed that transposition of the Tn3[lac+] element occurred and that its frequency was derepressed compared to frequencies reported by others for wild-type Tn3 transposition.
D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004274:DNA, Recombinant