title: Comparison of the occurrence of antibodies to human cytomegalovirus as demonstrated by complement fixation, enzyme-linked-immunosorbent assay and indirect immunofluorescence.
abstract: 180 serum specimens from pregnant women and renal transplant recipients, were studied for the presence of antibodies to human cytomegalovirus (HCMV) by complement fixation (CF), enzyme-linked-immunosorbent assay (EIA) for IgG and IgM, and indirect immunofluorescence (IIF). IIF was carried out separately against immediate-early, early and late HCMV-induced antigens. A positive CF test was always associated with the presence of antibodies against late virus antigens. EIA procedures were able to detect HCMV antibodies also when CF was negative and when IIF was positive only against immediate-early and/or early HCM-induced antigens.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D016030:Kidney Transplantation; D011247:Pregnancy
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M