Isolation and characterisation of a non-polyadenylated mRNA species with an affinity for poly(adenylic acid) from Friend leukaemia cells.
Utilizing the technique of poly(A)-Sepharose affinity chromatography, it is possible to isolate a novel class of RNA molecules from polysomes of Friend leukaemia cells. These RNA species display messenger RNA-like behaviour. They are released from polysomes on treatment with EDTA and are able to direct polypeptide synthesis in a cell-free protein synthesising system. They appear to be distinct from the polyadenylated mRNAs, as judged by their lack of a 3'-terminal poly(A) tract, by their different size distribution, by their unusual base composition, by the presence of a possible 'uridylate rich' region towards their 3'-end, by their low sequence homology to polyadenylated mRNAs and by the difference in at least some of their translation products.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D002999:Clone Cells; D005622:Friend murine leukemia virus; D007942:Leukemia, Experimental / Q000032:analysis*; D051379:Mice; D010452:Peptide Biosynthesis; D011061:Poly A / Q000378:metabolism; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A