title: Effects of high calcium solutions on glutamate sensitivity of crayfish muscle fibres.
abstract: Crayfish neuromuscular preparations were studied after 18--36 h exposure to high calcium solutions. As previously reported for frog neuromuscular preparations the treatment damaged the nerve terminals and decreased junctional potentials. The resting potentials and input resistances of the muscle fibres were not affected; but their sensitivity to glutamate was significantly decreased when compared to that of control muscles. After exposure to high calcium, the sensitivity to gamma-aminobutyric acid, the putative transmitter at inhibitory synapses, was increased. Apparently normal twitches were elicited by direct stimulation, and calcium spikes could still be observed in the fibres. A decreased sensitivity to glutamate was also noted in experiments carried out on denervated muscles 8 months after section of the motor axons. Possible relations between nerve terminal damage and the decrease in sensitivity to glutamate are discussed.
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology*; D004553:Electric Conductivity; D005971:Glutamates / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009121:Muscle Denervation; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000648:ultrastructure; D012996:Solutions; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D005971:Glutamates; D012996:Solutions; D005680:gamma-Aminobutyric Acid; D002118:Calcium