Enhanced recombination between lambda plac5 and F42lac: identification of cis- and trans-acting factors.
Enhanced transductional recombination between specialized transducing phage lambda plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D010957:Plasmids; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase