Effect of glutathione deficiency on the pool of CoA-glutathione mixed disulfide in Escherichia coli.
The formic acid extracts of several glutathione-deficient strains of Escherichia coli have been assayed for the presence of the mixed disulfide of CoA and glutathione, CoASSG. Strains deficient in gamma-glutamyl-cysteine synthase (EC 6.3.2.2) produced only CoA dimer. Strains deficient in glutathione synthase (EC 6.3.2.3) produced the mixed disulfide of CoA and the gamma-glutamylcysteine dipeptide. The pool size of total CoA in the cell did not change significantly even in the absence of glutathione.
D003065:Coenzyme A / Q000031:analogs & derivatives* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism*; D005981:Glutathione Synthase / Q000378:metabolism*; D046911:Macromolecular Substances; D009154:Mutation; D010453:Peptide Synthases / Q000378:metabolism*
D046911:Macromolecular Substances; C019305:coenzyme A-glutathione mixed disulfide; D010453:Peptide Synthases; D005721:Glutamate-Cysteine Ligase; D005981:Glutathione Synthase; D005978:Glutathione; D003065:Coenzyme A