Prostacyclin-independence in beta-adrenoceptor mediated renin release from dog renal cortical slices.
There is some controversy regarding whether stimulation of renin release by the beta-adrenergic system is dependent on prostaglandin (PG) production. We have examined this problem in renal cortical slices of the dog and have obtained the following results: (1) Isoproterenol (4 X 10(-6) M) stimulated renin release, but had no effect on the formation of 6-keto PGF1 alpha, a stable metabolite of PGI2; (2) Indomethacin (2 X 10(-5) M) had no effect on isoproterenol stimulated renin release, but inhibited 6-keto PGF1 alpha formation; (3) Dibutyryl cyclic AMP (10(-3) M) stimulated both renin release, and 6-keto PGF1 alpha release. Indomethacin (2 X 10(-5) M) did not inhibit dibutyryl cyclic AMP-stimulated renin release, but did inhibit the production of 6 keto PGF1 alpha. These results indicate that the beta-adrenoceptor mediated renin release does not depend on the formation of PGI2, but renin release is dependent on cyclic AMP formation.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D004285:Dogs; D011464:Epoprostenol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D011433:Propranolol / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D012083:Renin / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D015121:6-Ketoprostaglandin F1 alpha; D003994:Bucladesine; D011433:Propranolol; D011464:Epoprostenol; D012083:Renin; D007545:Isoproterenol; D007213:Indomethacin