Monoclonal antibodies to tyrosine hydroxylase: production and characterization.
Monoclonal antibodies were produced against tyrosine hydroxylase (TH), the enzyme catalyzing the rate limiting step in catecholamine neurotransmitter synthesis. An enzyme linked immunosorbant assay was used to detect the presence of specific antibody in culture. Specificity of the antibodies is characterized. These monospecific antibodies were used for immunoprecipitation and immunohistochemical staining of TH in brain.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000918:Antibody Specificity; D002421:Caudate Nucleus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D051381:Rats; D013378:Substantia Nigra / Q000276:immunology; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology*
D000906:Antibodies; D014446:Tyrosine 3-Monooxygenase