title: DNA amplification and an unstable arginine gene in Streptomyces lividans 66.
abstract: Streptomyces lividans 66 produced spontaneous chloramphenicol-sensitive mutants (CmlS) at a frequency of about 1% of spores. The CmlS mutant strains were very unstable, giving Arg- mutants at frequencies of about 25% of spores. All the Arg- mutants had amplified a particular 5.75 kb DNA fragment into tandem repeats of 250-500 copies per chromosome.
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity; D001120:Arginine / Q000378:metabolism*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005784:Gene Amplification; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001120:Arginine; D004262:DNA Restriction Enzymes