Preparation and analysis of reactive blue 2 bonded to silica via variable spacer groups.
A variety of aminoalkylated microparticulate porous silicas have been prepared and used to displace the reactive chloride from reactive blue 2 thereby immobilizing the dye in a mild and predictable manner. Methods of analysis of immobilized reactive blue 2 are compared and a rapid new method is recommended which is clean and non-destructive. The biospecific elution of a dehydrogenase from an immobilized dye column is demonstrated.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D012822:Silicon Dioxide; D014227:Triazines / Q000032:analysis*
D014227:Triazines; C012842:Cibacron Blue F 3GA; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase