Association of guanylate cyclase with the axoneme of retinal rods.
Axonemes were isolated from purified bovine retinal rod outer segments by dissolving the outer segment membranes in detergent and separating the axonemes by centrifugation on a linear detergent-containing sucrose density gradient. Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing), EC 4.61.2) activity was concentrated in the axoneme fraction. Guanylate cyclase eluted in the void volume when detergent-solubilized rod outer segments were subjected to exclusion chromatography on Sepharose 4B. Attempts to extract guanylate cyclase from isolated axonemes with salt, EDTA, base and other reagents were successful.
D000818:Animals; D002417:Cattle; D006162:Guanylate Cyclase / Q000302:isolation & purification*; D010786:Photoreceptor Cells / Q000201:enzymology* / Q000648:ultrastructure
D006162:Guanylate Cyclase