title: Growth conditions of F9 embryonal carcinoma cells affect the degree of DNA methylation.
abstract: We have investigated differences in C pG methylation between F9 embryonal carcinoma cells in vitro and as tumor cells grown in vivo using Msp I and Hpa II restriction isoschizomers. Southerns were hybridized with two low copy number probes, mouse major beta-globin (f7) and a class I, histocompatibility-2 cDNA clone (pH-2d-4). In each case, the tumor-DNA was hypomethylated while the DNA from F9 cells grown in vitro was moderately methylated. We conclude that growth conditions or cell-cell interactions can greatly affect methylation of C pG sites.
mesh_terms: D044503:5-Methylcytosine; D000818:Animals; D002478:Cells, Cultured; D003596:Cytosine / Q000031:analogs & derivatives; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000235:genetics; D008297:Male; D008745:Methylation; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D013724:Teratoma / Q000235:genetics / Q000378:metabolism*
chemical_list: D004273:DNA, Neoplasm; D006183:H-2 Antigens; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes