Conversion of 14C-labeled eicosapentaenoic acid (n-3) to leukotriene C5.
14C-labeled eicosapentaenoic acid (n-3) was converted by mouse mastocytoma cells to 5-hydroxy-6-S-glutathionyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene C5). The identification was based on comparisons with previously characterized unlabeled material by high-performance liquid chromatography, ultraviolet spectroscopy, and conversion by gamma-glutamyl transpeptidase to leukotriene D5.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005231:Fatty Acids, Unsaturated; D013189:SRS-A; C026144:leukotriene C-5; D015118:Eicosapentaenoic Acid; D005723:gamma-Glutamyltransferase