A comparison of IHA and ELISA tests in the detection of antibodies to entamoeba histolytica.
In this report we have evaluated the sensitivity and specificity of IHA and ELISA for the detection of anti-amebic antibodies in the serum samples of 147 patients with poor mental and hygienic status hospitalized in mental institutions. Our data have demonstrated a positive agreement between ELISA and IHA on positive sera. The major drawback of IHA is the occurrence of false negative results, 13/82 sera negative in IHA (less than 1/128) are positive in the ELISA. Furthermore, the ELISA is useful for serological diagnosis of amebiasis in the laboratories of low endemic areas.
D000906:Antibodies / Q000032:analysis*; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques
D000906:Antibodies