Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005822:Genetic Vectors; D009154:Mutation; D010957:Plasmids; D013997:Time Factors; D014162:Transfection
D005020:Ethyl Methanesulfonate