title: An overall radioassay for the first three reactions of de novo pyrimidine biosynthesis.
mesh_terms: D000581:Amidohydrolases / Q000032:analysis*; D001221:Aspartate Carbamoyltransferase / Q000032:analysis*; D001224:Aspartic Acid; D001639:Bicarbonates; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000032:analysis*; D002221:Carbamyl Phosphate; D002250:Carbon Radioisotopes; D003013:Clostridium / Q000201:enzymology; D004080:Dihydroorotase / Q000032:analysis*; D013293:Enterococcus faecalis / Q000201:enzymology; D007700:Kinetics; D008025:Ligases / Q000032:analysis*; D011743:Pyrimidines / Q000096:biosynthesis*; D011865:Radioisotope Dilution Technique
chemical_list: D001639:Bicarbonates; D002250:Carbon Radioisotopes; D011743:Pyrimidines; D001224:Aspartic Acid; D002221:Carbamyl Phosphate; D001221:Aspartate Carbamoyltransferase; D000581:Amidohydrolases; D004080:Dihydroorotase; D008025:Ligases; D002222:Carbamoyl-Phosphate Synthase (Ammonia)