Collagenase perfusion of rat liver induces DNA damage and DNA repair in hepatocytes.
Evidence is presented that the collagenase perfusion of adult rat liver results in significant damage to nuclear DNA as evaluated by the alkaline elution technique. The extent of the damage is related to the perfusion time as well as to the clostridial enzyme preparation used. The DNA structure of isolated cells is almost completely repaired within 12 h of their culture in chemically defined medium.
D000818:Animals; D004260:DNA Repair; D008099:Liver / Q000187:drug effects* / Q000502:physiology; D008297:Male; D003012:Microbial Collagenase / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D003012:Microbial Collagenase