Properties of peroxisomal 3-ketoacyl-coA thiolase from rat liver.
Peroxisomal 3-ketoacyl-CoA thiolase has a molecular weight of 89,000 and consists of 2 polypeptide chains of identical size. The enzyme has no interchain disulfide bonds and is reversibly dissociated to an inactive monomer in the cold. Mitochondrial 3-ketoacyl-CoA thiolase and acetoacetyl-CoA specific thiolase have molecular weights of 154,000 and 149,000, respectively. They each consist of 4 polypeptide chains of identical size. Peroxisomal thiolase and mitochondrial 3-ketoacyl-CoA thiolase operate by a ping-pong mechanism. The catalytic properties, including substrate specificity, of the peroxisomal enzyme were compared to those of mitochondrial 3-ketoacyl-CoA thiolase.
D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D000102:Acetyl-CoA C-Acyltransferase / Q000032:analysis / Q000378:metabolism*; D000217:Acyltransferases / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003080:Cold Temperature; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008830:Microbodies / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D008970:Molecular Weight; D009940:Organoids / Q000201:enzymology*; D051381:Rats; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D013438:Sulfhydryl Compounds; D000217:Acyltransferases; D000102:Acetyl-CoA C-Acyltransferase; D000101:Acetyl-CoA C-Acetyltransferase; D014357:Trypsin