High-pressure liquid chromatography of nadolol and other beta-adrenergic blocking drugs.
A high-pressure liquid chromatographic assay was developed for the analysis of the beta-adrenergic blocking agent nadolol as a bulk material or formulated in a tablet. Other beta-adrenergic blocking drugs such as acebutolol, alprenolol, atenolol, metoprolol, oxprenolol, pindolol, practolol, propranolol, sotalol, and timolol can be chromatographed in this system. An ethylsilane column and a mobile phase consisting of 35% methanol-65% aqueous 0.0005 M hydrochloric acid-0.05 M sodium chloride are used. Detection is either at 254 nm with a variable-wavelength detector. As exemplified by nadolol, the drug content can be quantitated with or without atenolol as an internal standard.
D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D009248:Nadolol; D011412:Propanolamines / Q000032:analysis*; D012015:Reference Standards; D013607:Tablets
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; D013607:Tablets; D009248:Nadolol