title: Failure of somatostatin and beta-endorphin to affect bovine adrenal cortex cells in vitro.
abstract: The main intention of this study was to examine the effect of cyclic somatostatin (SRIF) on bovine adrenal cell activity in vitro. beta-Endorphin was applied in another course of experiments. Basal aldosterone secretion when measured was found to be 0.86 +/- 0.33 ng per 10(5) cells and 1.5 h incubation time in the glomerulosa cell fractions. Cortisol production was 1.6 +/- 0.3 ng per 10(5) cells in the fasciculata/reticularis cell incubates respectively. No effect was observed when cyclic somatostatin (SRIF) was added to cell incubates of the bovine adrenal cortex. However, aldosterone secretion was increased 4.5-fold in the presence of 10(-9)M ACTH or 10(-8)M At II. Cortisol production was enhanced similarly after addition of 10(-9)M ACTH.
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004723:Endorphins / Q000494:pharmacology*; D006854:Hydrocortisone / Q000378:metabolism*; D013004:Somatostatin / Q000494:pharmacology*; D001615:beta-Endorphin
chemical_list: D004723:Endorphins; D000450:Aldosterone; D013004:Somatostatin; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone