Demonstration with monoclonal antibody of the glycoprotein nature of Thy-1.2 alloantigen.
Thy-1 antigen present in large amounts on the brain and thymus of mice, exists in two allelic forms, either Thy-1.1 or Thy-1.2. Previous results have shown that Thy-1.1 alloantigen is expressed on a glycoprotein of molecular weight 25 000, therefore referred to as the Thy-1 glycoprotein. However the presence of Thy-1.2 alloantigen on the purified Thy-1 glycoprotein could not be established unequivocally. The present paper demonstrates that Thy-1.2 alloantigen, identified by F7D5 monoclonal antibody, is localized on the Thy-1 glycoprotein. The limited neutralization by the glycoprotein of the reactivity of AKR anti-C3H allosera to thymocytes is explained by the fact that the antiserum contains antibodies to determinants other than Thy-1.2. The alloantisera are shown to react with viral proteins encoded for by endogenous murine leukemia virus and expressed at the surface of Friend cells and thymocytes.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D007700:Kinetics; D007942:Leukemia, Experimental / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D006023:Glycoproteins; D008565:Membrane Proteins; D018800:Thy-1 Antigens