Neutralization of heat-labile toxin of E. coli by antibodies to synthetic peptides derived from the B subunit of cholera toxin.
Antibodies elicited by six synthetic peptides corresponding to various fragments of B subunit of cholera toxin (CT) were evaluated for their cross-reactivity with heat-labile toxin (LT) of Escherichia coli. The antiserum directed towards the peptide CTP3 (residues 50-64) was found highly cross-reactive with the LT, in radioimmunoassay and immunoblotting. This peptide was also the most cross-reactive with intact CT. The antiserum against CTP1 (residues 8-20) was also cross-reactive with the two toxins, although to a much lower extent. Antisera to both CTP1 and CTP3, which are inhibitory towards CT, were found equally effective in neutralizing the biological activity of the E. coli LT. This was manifested by inhibition of both adenylate cyclase activity and fluid secretion into ligated ileal loops of rats. These results might indicate the potential of such synthetic peptides as the basis for a general vaccine against several types of infectious diarrhea.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins / Q000276:immunology* / Q000494:pharmacology; D002645:Chickens; D002772:Cholera Toxin / Q000276:immunology*; D003429:Cross Reactions; D000242:Cyclic AMP / Q000378:metabolism; D004768:Enterotoxins / Q000276:immunology* / Q000494:pharmacology; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins; D007082:Ileum / Q000187:drug effects; D007106:Immune Sera; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D046911:Macromolecular Substances; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007106:Immune Sera; D046911:Macromolecular Substances; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli; D000242:Cyclic AMP; D000262:Adenylyl Cyclases