A one-step liquid-chromatographic technique for the estimation of the deoxythymidine-5'-triphosphatase in human serum.
Deoxythymidine-5'-triphosphatase (dTTPase) from human serum was separated by DEAE-cellulose chromatography from unspecific hydrolases in a single step. The method was adapted to a microscale and the enzymatic activity was determined for five different groups of patients including epithelial carcinoma, leukemia and three control groups. The result is that the purified dTTPase preparations of these groups reflect the situation obtained in the whole serum.
D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007700:Kinetics; D010744:Phosphoric Monoester Hydrolases / Q000097:blood*; D011755:Pyrophosphatases
D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; C023117:thymidine-triphosphatase