Isolation and identification of enteroviruses from faecal samples in a differentiated epithelial cell line (HRT-18) derived from human rectal carcinoma.
A human rectal carcinoma-derived differentiated epithelial cell line, HRT-18, was inoculated with faecal samples in an attempt to grow coronavirus-like particles (CVLP), which are widely prevalent in human stools in southern India. While CVLP did not grow in this cell line, a variety of enteroviruses were isolated from 48 of the 114 stool samples from healthy controls and patients with diarrhoea. The results suggest that human gastrointestinal tumour-derived differentiated epithelial cells in continuous culture may be useful for the primary isolation of enteroviruses and merit further study.
D000328:Adult; D002460:Cell Line; D002648:Child; D003967:Diarrhea / Q000382:microbiology; D004770:Enterovirus / Q000254:growth & development / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000382:microbiology; D004848:Epithelium; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012004:Rectal Neoplasms; D014776:Virus Cultivation