Phospholipid metabolism in V79-R membranes composed of phospholipid molecular species containing trans-monoenoic fatty acids.
The interrelationship between the inhibition of cell growth and changes in phospholipid molecular species was studied in the presence of elaidic, trans-11-eicosenoic, or brassidic acids in Chinese hamster V79-R cells. The addition of trans-monoenoic fatty acids to the medium inhibited cell growth and caused an increase in the total cellular content of phospholipids. However, there was no difference in the polar head group composition of these phospholipids among all the cells supplemented with trans-monoenoic fatty acids. Exogenous trans-monoenoic fatty acids were incorporated into cellular phospholipids to form novel phospholipid molecular species. Phospholipid synthesizing enzyme activities bound to the membranes composed of phospholipid molecular species of trans-monoenoic fatty acids were determined. Cholinephosphotransferase [EC 2.7.8.2] and ethanolaminephosphotransferase [EC 2.7.8.1] activities were decreased by trans-11-eicosenoic acid, but not changed by elaidic acid. Glycerophosphate acyltransferase [EC 2.3.1.15] activity was increased by elaidic acid and decreased by trans-11-eicosenoic acid. Cholinephosphate cytidylyltransferase [EC 2.7.7.15] activity was not changed by trans-monoenoic fatty acids.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D019916:Choline-Phosphate Cytidylyltransferase; D006224:Cricetinae; D003412:Cricetulus; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D004982:Ethanolaminephosphotransferase / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D007700:Kinetics; D008168:Lung; D008563:Membrane Lipids / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism*; D013329:Structure-Activity Relationship
D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D010743:Phospholipids; D005992:Glycerol-3-Phosphate O-Acyltransferase; D009713:Nucleotidyltransferases; D019916:Choline-Phosphate Cytidylyltransferase; D004982:Ethanolaminephosphotransferase; D002798:Diacylglycerol Cholinephosphotransferase