Prostaglandins and cyclic AMP stimulate creatine kinase synthesis but not fusion in cultured embryonic chick muscle cells.
Cyclic AMP levels have been measured in cultures derived from 12-day-old chick embryonic muscle. A rise in concentration was found after the onset of myoblast fusion. Cells cultured at a medium Ca2+ concentration of 0.1 microM did not fuse and exhibited only a small rise in cyclic AMP concentration during culture. Addition of 1.4 mM Ca2+ to these cells after 50 h in culture caused rapid, synchronous fusion with a concomitant rise in cyclic AMP levels. Indomethacin, an inhibitor of prostaglandin synthesis, did not inhibit fusion, but inhibited the rise in cyclic AMP concentration. Indomethacin-treated cultures exhibited lower creatine kinase levels, though no change in the ratio of the three isoenzymes was observed. Addition of prostaglandins E1 and E2 to indomethacin-treated cultures overcame this inhibition. We propose that prostaglandin synthesis is a consequence of the stimulation of myoblast fusion and that via cyclic AMP it stimulates protein synthesis.
D000527:Alprostadil; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology*; D015232:Dinoprostone; D007213:Indomethacin / Q000494:pharmacology; D007527:Isoenzymes; D007700:Kinetics; D009132:Muscles / Q000196:embryology* / Q000201:enzymology; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology
D007527:Isoenzymes; D011453:Prostaglandins; D011458:Prostaglandins E; D000242:Cyclic AMP; D003402:Creatine Kinase; D000527:Alprostadil; D015232:Dinoprostone; D002118:Calcium; D007213:Indomethacin