Adult rat oligodendrocytes grown in vitro upon an extracellular matrix have the ability to proliferate.
The use of extracellular matrix (ECM) as a natural substrate for cell culture has markedly improved the growth and morphological differentiation of isolated adult rat oligodendrocytes. ECM-grown oligodendrocytes exhibited cyclic nucleotide phosphodiesterase (CNP)-activity which increased with time in culture and network formation. As much as 50-70% of the cells incorporated [3H]thymidine as visualized by the high labeling index of galactocerebroside (GalC)-positive cells. Chemical and enzymatic modifications of the ECM suggested that laminin in conjunction with other ECM constituents, plays a role in the induction of proliferation and/or differentiation responses in mature oligodendrocytes.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D005109:Extracellular Matrix; D005699:Galactosylceramides / Q000378:metabolism; D007797:Laminin; D009457:Neuroglia; D009836:Oligodendroglia; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D005699:Galactosylceramides; D007797:Laminin; D003094:Collagen; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D013936:Thymidine