Decline in infectivity of simian virus 40 by sodium deoxycholate and its restoration with the extract of monkey kidney cells.
Plaque-forming activity and T-antigen-synthesizing activity in the crude preparation of simian virus 40 (SV40) decreased to 1/20-27 after treatment with 0.5% sodium deoxycholate (DOC) for 30 min at 37 degrees C. A full restoration of the activity occurred after incubation of DOC-treated virions with the extract of monkey CV-1 cells, host cells for productive infection with SV40. Analysis by sedimentation through 15% sucrose to CsCl cushion (rho = 1.327 g/cm3) revealed that virions in the [35S]methionine-labeled crude virus preparation sedimented to the interface between CsCl and sucrose, and that treatment with DOC resulted in the loss of infectivity and the appearance of virions sedimentable into CsCl cushion. The [35S]methionine-labeled purified virions (prepared after treatment with DOC and sedimentable into CsCl cushion) sedimented to the CsCl-sucrose interface after incubation with the cell extract, with restoration of infectivity. The infectivity-restoring activity of the cell extract was sensitive to ethyl ether, partially sensitive to heating at 75 degrees-97 degrees for 30 min, but resistant to treatment with DNase (50 micrograms/ml), RNase (40 micrograms/ml), or trypsin (0.05%) for 30 min at 37 degrees. These results suggest that lipid-related cellular components bind stably to virions of SV40 and facilitate an efficient infection.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003840:Deoxycholic Acid / Q000494:pharmacology*; D007668:Kidney / Q000502:physiology*; D007700:Kinetics; D013539:Simian virus 40 / Q000187:drug effects / Q000235:genetics*; D014020:Tissue Extracts / Q000494:pharmacology*; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000187:drug effects / Q000235:genetics
D014020:Tissue Extracts; D014764:Viral Proteins; D003840:Deoxycholic Acid