Comparative cytogenetic study of a continuous human cell line and its subline susceptible and resistant, respectively, to coxsackie B3 virus.
Cytogenetic characteristics of the J-96 human cell line and its J-41 subline, highly susceptible and resistant, respectively, to coxsackie B3 virus, were compared. The J-41 subline, as compared to the original J-96 line, had fewer chromosomes in the modal class cells (54-57 and 58-62, respectively) mostly at the expense of normal chromosomes. In most J-41 cells chromosome 21 was eliminated and the number of homologues of chromosomes 2, 9, 11, and 12 was reduced. The percentage of marker chromosomes in the J-41 subline (31.3) was slightly higher than in the J-96 line (24.3). The relationship between differences in the karyotypes and properties of the cultures such as resistance to coxsackie B3 virus, capacity to produce virus-induced interferon and to acquire an antiviral state after treatment with interferon were discussed.
D002460:Cell Line; D002877:Chromosomes, Human; D029822:Enterovirus B, Human / Q000254:growth & development*; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid
D005819:Genetic Markers