title: Characterization of thermotropic structural transitions of the erythrocyte membrane: a biochemical and electron-paramagnetic resonance approach.
abstract: The relationship between membrane structural properties and functions has been generally inferred from observed thermotropic phenomena. By the use of 16-dinyloxyl stearic acid spin probe we investigated the red blood cell membrane components involved in three characteristic thermotropic structural transitions occurring at 8, 20, and 40 degrees C. The transition at 8 degrees C is removed by chymotrypsin treatment at the cytoplasmic membrane layer. The 20 degrees C phase transition is unmodified after chymotrypsin treatment and occurs at 15 degrees C after complete proteolysis of intramembrane chymotrypsin-insensitive peptides. Liposomes from the total lipid extract of RBC show only one thermotropic transition at 15 degrees C. The 40 degrees C phase transition is absent in vesicles free of skeletal proteins, in vesicles obtained after RBC storage, and in low-ionic-strength resealed ghosts. Transitions at 8 degrees C and 40 degrees C appear to be due to the interactions of cytoplasmic exposed proteins with membrane, whereas the 20 degrees C transition is intrinsic to the lipid component.
mesh_terms: D002918:Chymotrypsin / Q000494:pharmacology; D003497:Cyclic N-Oxides; D003593:Cytoplasm / Q000502:physiology; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000502:physiology*; D006801:Humans; D007038:Hypotonic Solutions; D008081:Liposomes; D008563:Membrane Lipids / Q000502:physiology; D012965:Sodium Chloride; D013113:Spin Labels; D013696:Temperature
chemical_list: D003497:Cyclic N-Oxides; D007038:Hypotonic Solutions; D008081:Liposomes; D008563:Membrane Lipids; D013113:Spin Labels; D012965:Sodium Chloride; C032363:16-nitroxystearic acid; D002918:Chymotrypsin