[Various properties of galactose oxidase from Fusarium graminearum IMV-F-1060 immobilized on aminoorganosilochromes].
Galactose oxidase preparations are obtained from Fusarium graminearum IMV-F-N 1060 immobilized on aminoorganosilochromes activated by cyanuron chloride and 2.4-toluylene diizocyanate. The immobilized preparations were studied for their selective action on different carbohydrate substrates and for the pH-medium dependence of the obtained preparation activity. Potassium ferricyanide is established to have an activating effect on the immobilized enzyme. It is shown that the immobilized galactose oxidase preparations may be used for the analysis of galactose and lactose.
D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000302:isolation & purification / Q000378:metabolism*; D005292:Ferricyanides / Q000494:pharmacology; D005670:Fusarium / Q000201:enzymology*; D005692:Galactose Oxidase / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012822:Silicon Dioxide; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D005292:Ferricyanides; D012822:Silicon Dioxide; C016999:aminosilochrome; D005692:Galactose Oxidase; C028033:potassium ferricyanide
Nekotorye svoĭstva galaktozooksidazy iz Fusarium graminearum IMB-F-No. 1060, immobilizovannoĭ na aminoorganosilokhromakh.