title: Effects of various forms of stimulation on the content of enolase isozymes and S-100 protein in superior cervical sympathetic ganglia excised from rats.
abstract: Contents of the three forms (alpha alpha, alpha gamma, and gamma gamma) of enolase isozymes and S-100 protein in superior cervical sympathetic ganglia (SCG) excised from rats were determined by the sensitive method of enzyme immunoassay, after application of various forms of stimulation, during incubation for 3 h at 37 degrees C in vitro. The amounts of the three forms of enolase isozymes and of S-100 protein in the SCG were not altered by preganglionic or postganglionic stimulation (10 Hz) or by the addition of acetylcholine (1 mM) or a high concentration of K+ (70 mM) to the incubation medium. Norepinephrine (NE; 50 microM), as well as isoproterenol (200 microM) or 3,4-dihydroxy phenylethylamine (dopamine; 200 microM), increased the ganglionic alpha alpha and alpha gamma enolase content to 1.5 to 2.0 times the control level, whereas NE tended to slightly decrease the gamma gamma enolase content. The increase in the alpha isozymes did not appear until after 2 to 3 h of incubation with this agent as a result of an increase in protein synthesis. Propranolol, an adrenergic antagonist, partly inhibited the NE-induced increase in both alpha alpha and alpha gamma enolases. NE and its agonists also considerably increased the S-100 protein level in the SCG; however, the effect developed within half an hour of incubation as a result of the conversion of the bound S-100 protein to the water-soluble form, and did not greatly increase thereafter. Cyclic AMP (1 mM) produced the same kind of increase in the ganglionic S-100 protein content as NE did.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology; D003714:Denervation; D004298:Dopamine / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D009418:S100 Proteins / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D009418:S100 Proteins; D000242:Cyclic AMP; D010751:Phosphopyruvate Hydratase; D007545:Isoproterenol; D000109:Acetylcholine; D011188:Potassium; D004298:Dopamine; D009638:Norepinephrine