title: Primary monolayer culture of liver parenchymal cells and kidney cortical tubules as a useful new model for biochemical pharmacology and experimental toxicology. Studies in vitro on hepatic membrane transport, induction of liver enzymes, and adaptive changes in renal cortical enzymes.
abstract: Freshly isolated liver parenchymal cells were maintained in either short-term monolayer, suspension of long-term monolayer culture. Rapidly occurring processes through hepatocellular membrane, e.g., the enhanced amino acid transport and the concomitantly increased potassium influx following progressive starvation, were kinetically evaluated best in short-term monolayer culture. The inducibility of tyrosine aminotransferase by glucagon, dexamethasone, and a combination of both was compared in suspension and in monolayer culture. The induction of slowly inducible foreign compound-metabolizing enzymes, (e.g., ethoxycoumarin-O-dealkylase, p-nitroanisole-O-demethylase, and UDP-glucuronyltransferase) by phenobarbital, 3-methylcholanthrene, and dexamethasone were studied in long-term monolayer culture. The latter system was also used to maintain isolated kidney cortical tubules for the investigation of renal enzyme adaptation during progressive time in culture.
mesh_terms: D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004790:Enzyme Induction; D007672:Kidney Cortex / Q000166:cytology* / Q000201:enzymology; D007684:Kidney Tubules / Q000166:cytology*; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008297:Male; D008566:Membranes / Q000378:metabolism; D010600:Pharmacology / Q000379:methods; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012413:Rubidium / Q000378:metabolism; D014116:Toxicology / Q000379:methods; D014444:Tyrosine Transaminase / Q000378:metabolism
chemical_list: D000621:Aminoisobutyric Acids; D014444:Tyrosine Transaminase; D012413:Rubidium; D011188:Potassium