New enzyme immunoassays for measurement of influenza A/Victoria/3/75 virus in nasal washes.
Nasopharyngeal (NP) washes from 12 patients experimentally infected with influenza A/Victoria/3/75 wild-type virus were tested in 2 newly developed enzyme-immunoassay (EIA) systems. Results were compared with infectivity titres of the NP wash specimens in rhesus monkey kidney-tissue culture. EIA detected A/Victoria/3/75 haemagglutinin antigen for more days than did culture. EIA is also rapid and easy to do in large numbers; hence it may have advantages over culture methods for the diagnosis of influenza infections and the investigation of influenza epidemiology.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D046508:Culture Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005122:Exudates and Transudates / Q000382:microbiology; D000882:Haplorhini; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000382:microbiology*; D007668:Kidney; D008253:Macaca mulatta; D009305:Nasopharynx / Q000382:microbiology*; D007507:Therapeutic Irrigation
D000956:Antigens, Viral