Characteristics of polymorphonuclear leukocyte infiltration into the alkali burned eye and the influence of sodium citrate.
Polymorphonuclear leukocytes (PMNs) are considered to play a central role in the corneal ulceration process subsequent to an alkali burn. We have described the time course of PMN infiltration into the ocular tissues following an alkali burn. In addition, we examined the effect of sodium citrate upon the accumulation of PMNs in the alkali burned cornea. The accumulation of PMNs into the cornea and iris-ciliary body was quantified by measuring myeloperoxidase (MPO), an enzyme marker for these inflammatory cells. Leukocytes in aqueous humor aspirates and corneal washes were counted directly under a microscope. In the alkali burned cornea, we found an initial transient, yet substantial, infiltration of PMNs, peaking at about 12-24 hr and limited to the peripheral cornea; this subsided by about 72 hr. By 14 days, the MPO activity, and hence the number of leukocytes, had risen again, and by 21 days the level had increased by several fold. Qualitatively similar biphasic patterns of leukocyte infiltration were observed in the iris-ciliary body and aqueous humor. Leukocyte numbers in corneal washes only increased between 4-24 hr following the alkali burn. The exceptionally high degree of leukocyte infiltration into the cornea at 21 days corresponded with the presence of ulceration. Topical administration of sodium citrate (10%) inhibited the early and late phase of PMN accumulation in the alkali burned cornea, i.e. at 24 hr (-63%) and 21 days (-92%). The inhibition of PMN infiltration by sodium citrate correlates with the reduced corneal ulceration observed following treatment with this compound.
D000818:Animals; D000863:Antacids / Q000627:therapeutic use*; D002057:Burns, Chemical / Q000473:pathology*; D002465:Cell Movement / Q000187:drug effects; D002951:Citrates / Q000627:therapeutic use*; D019343:Citric Acid; D003315:Cornea / Q000201:enzymology / Q000473:pathology; D065306:Corneal Injuries; D005126:Eye Burns / Q000139:chemically induced / Q000473:pathology*; D005260:Female; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits; D012972:Sodium Hydroxide; D013997:Time Factors
D000863:Antacids; D002951:Citrates; D019343:Citric Acid; D012972:Sodium Hydroxide; D009195:Peroxidase