Cell-mediated synthesis of somatostatin.
Messenger RNA from catfish pancreatic islets was translated in a wheat germ cell-free protein synthesizing system. A protein of 12,000 mol wt, preprosomatostatin, was identified by specific immunoprecipitaton with anti-catfish pancreatic somatostatin and sodium dodecyl sulfate (SDS)- polyacrylamide gel electrophoresis.
D000818:Animals; D002474:Cell-Free System; D005399:Fishes; D007118:Immunoassay; D007515:Islets of Langerhans / Q000378:metabolism*; D008970:Molecular Weight; D010944:Plants / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D013004:Somatostatin / Q000096:biosynthesis*; D014908:Triticum / Q000378:metabolism
D012333:RNA, Messenger; D013004:Somatostatin