Cloning of SV40 genomes from human brain tumors.
From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early region removing most of the large T-coding region. In addition, this mutant also carries a tandem duplication of an intact origin of replication.
D001254:Astrocytoma / Q000382:microbiology*; D001932:Brain Neoplasms / Q000382:microbiology*; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008579:Meningioma / Q000382:microbiology*; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D004262:DNA Restriction Enzymes