Immunochemistry of 7 S domain of type IV collagen.
The 7 S domain of type IV collagen was isolated from various basement membranes by pepsin and bacterial collagenase digestion. The antibodies were raised in rabbits against human placental 7 S and bovine cortex 7 S. The antisera were studied for their binding to 7 S samples. The results showed a lack of tissue specificity to 7 S from the same species. In contrast no cross-reactivity was found between 7 S from different species. A set of peptides from 7 S were also prepared and tested for their binding to antiserum, showing retention of some antigenic sites.
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000032:analysis; D003094:Collagen / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007106:Immune Sera; D007668:Kidney / Q000032:analysis; D007908:Lens, Crystalline / Q000032:analysis; D008297:Male; D003012:Microbial Collagenase; D008970:Molecular Weight; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D013552:Swine
D000596:Amino Acids; D007106:Immune Sera; D010446:Peptide Fragments; D003094:Collagen; D010434:Pepsin A; D003012:Microbial Collagenase