[Affinity labeling of inorganic pyrophosphatase from yeast by methylphosphate].
Yeast inorganic pyrophosphatase is specifically and irreversibly inactivated by methylphosphate. The high rate of inhibition, the protective effect of the substrate, the strict correlation between the degree of inhibition and the amount of the protein-bound reagent and the effect of saturation of the enzyme with methylphosphate provide evidence in favour of the reaction in the active center. Modification of two chemically identical enzyme subunits proceeds at different rates and results in a formation of phosphorylated subunits with different stability of the phosphate bond, which is indicative of the mutual effects of the pyrophosphatase subunits. The reaction between the modified enzyme and hydroxylamine suggests that the interaction between pyrophosphatase and methylphosphate entails modification of the carboxylic groups of two active centers, resulting in a formation of the acylphosphate bonds.
D000345:Affinity Labels / Q000494:pharmacology*; D006898:Hydroxylamines / Q000494:pharmacology; D043564:Inorganic Pyrophosphatase; D007700:Kinetics; D046911:Macromolecular Substances; D010755:Organophosphates / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D011485:Protein Binding; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000345:Affinity Labels; D006898:Hydroxylamines; D046911:Macromolecular Substances; D010755:Organophosphates; D009943:Organophosphorus Compounds; C027265:methylphosphate; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase
Affinnaia modifikatsiia neorganicheskoĭ pirofosfatazy iz dorzhzheĭ metilfosfatom.