title: Molecular cloning and the complete nucleotide sequence of the creatine kinase-M cDNA from chicken.
abstract: The nucleotide sequence of creatine kinase-M (CK-M) cDNA clones has been determined. It includes the entire coding region of 381 amino acids in addition to 5' and 3' untranslated regions. A comparison with a partial sequence from rat CK-M reveals 84% nucleotide sequence homology in the coding region but divergence in the 3' untranslated region. The amino acid sequence is 94% conserved between chicken and rat. Hybridization to RNA immobilized on filters indicates homology between the CK-M 3' untranslated region and additional muscle specific RNA species. The coding region hybridizes only to CK-M RNA.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000235:genetics*; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005796:Genes; D007527:Isoenzymes; D009693:Nucleic Acid Hybridization
chemical_list: D007527:Isoenzymes; D004247:DNA; D003402:Creatine Kinase; D004262:DNA Restriction Enzymes