title: Cortisol inhibits the ACTH-releasing activity of urotensin I, CRF and sauvagine observed with superfused goldfish pituitary cells.
abstract: The structurally homologous peptides urotensin I, ovine CRF and sauvagine stimulate the release of immunoreactive ACTH from a superfused dispersed goldfish anterior pituitary cell column. The addition of cortisol to the superfusion buffer resulted, following a latent period, in a decrease in basal release of ACTH from the pituitary cell column and a diminution in the ACTH-releasing activities of urotensin I, CRF and sauvagine. The removal of cortisol from the superfusion buffer resulted in a slow recovery of basal ACTH release and a recovery of the ACTH-releasing activities of urotensin I, CRF and sauvagine. These results are supportive of the view that urotensin I, or a urotensin I-like peptide, serves as a physiological regulator of ACTH release in teleost fishes.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D029845:Amphibian Proteins; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D004357:Drug Synergism; D006054:Goldfish; D006854:Hydrocortisone / Q000494:pharmacology*; D036361:Peptide Hormones; D010455:Peptides / Q000494:pharmacology*; D010477:Perfusion; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014579:Urotensins / Q000494:pharmacology*
chemical_list: D029845:Amphibian Proteins; D036361:Peptide Hormones; D010455:Peptides; D014579:Urotensins; C025727:sauvagine; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I; D006854:Hydrocortisone