title: Entamoeba histolytica: collagenolytic activity and virulence.
abstract: Several axenic strains of pathogenic and nonpathogenic Entamoeba histolytica were tested for their capacity to digest native radioactive type I collagen gels and to produce liver abscesses when injected into the liver of newborn hamsters. The results demonstrate that the pathogenic strains of amebas (HM1:IMSS, HM3:IMSS, HM38:IMSS and HK9) have a collagenolytic activity that closely correlates with their in vivo capacity to produce liver lesions. The nonpathogenic isolate (Laredo) did not show collagenolytic activity and failed to produce lesions in the liver of newborn hamsters. The results also demonstrate that type I collagen obtained from rodents and cats is degraded less by amebic collagenase than is bovine collagen, which is similar to human collagen. These findings suggest that species susceptibility to invasive infection may depend, among other factors, on the characteristics of the extracellular components of host tissues.
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D003094:Collagen / Q000378:metabolism; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000201:enzymology / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000469:parasitology; D006168:Guinea Pigs; D008100:Liver Abscess / Q000209:etiology; D003012:Microbial Collagenase / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D014774:Virulence
chemical_list: D003094:Collagen; D003012:Microbial Collagenase