Development of GABAergic function of dissociated hippocampal cultures from fetal mice.
The GABA uptake mechanism has been characterized in hippocampal cell cultures prepared from fetal mice of 13-19 days gestational age [3H]GABA is accumulated selectively by neuronal cells by a high-affinity (Km = 3 micro M) mechanism that is an early property of the neurons. Autoradiography of [3H]GABA uptake revealed that approximately 30% of the neuronal cells had uptake. A unique small neuronal type did not have [3H]GABA uptake but was found to have [3H]glutamic acid uptake, suggesting that these neurons may be granule cells. The selective labeling of neurons with [3H]GABA and [3H]glutamic acid is consistent with the idea that high-affinity uptake of a transmitter may be unique to neurons that use that transmitter.
D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D002454:Cell Differentiation; D002794:Choline / Q000378:metabolism; D046508:Culture Techniques; D005260:Female; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology*; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000166:cytology; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005971:Glutamates; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D002794:Choline