Glycosidases from the rat uterus.
Six glycosidase activities were detected in homogenates of the rat uterus by using the p-Nitrophenyl-glycoside as substrate. The enzyme activities were separated by Sephadex G-200 column chromatography. The uterus contained alpha-galactosidase, beta-galactosidase and N-acetylglucosaminidase activity which had a similar pH optima of 4.4, whereas the pH optimum for beta-fucosidase was found to be of 4.4 - 4.6. The alpha-mannosidase activity showed two peaks of maximal activity at pH 5.0 and pH 6.0. The uterus contained two forms of the alpha-L-fucosidase activity separable by Sephadex G-200 which had a pH optima of 4.4 - 4.6 (form I) and pH 5.0 (form II). The glycosidases differed in heat stability, Km values and other several characteristics, which suggests that each enzyme apparently did not show any cross-contamination.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D004355:Drug Stability; D005260:Female; D005959:Glucosidases / Q000302:isolation & purification / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D006358:Hot Temperature; D008361:Mannosidases / Q000378:metabolism; D051381:Rats; D014599:Uterus / Q000201:enzymology*; D000519:alpha-Galactosidase / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D005959:Glucosidases; D006596:Hexosaminidases; D008361:Mannosidases; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase