title: Polypeptide involved in the Escherichia coli plasmid-mediated citrate transport system.
abstract: A genetic determinant conferring on Escherichia coli the ability to utilize citrate as a sole source of carbon and energy was subcloned into pBR322 from a naturally occurring, citrate utilization (Cit+) plasmid, pOH30221, and was localized to a 1.6-kilobase region by cloning and subsequent deletion analysis. Genetic expression of the Cit+ determinant in E. coli minicells revealed that the Cit+ determinant encoded a single, membrane-associated polypeptide with an apparent molecular weight of 35,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This polypeptide seemed not to be synthesized as a precursor with an amino-terminal signal sequence.
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D007700:Kinetics; D010455:Peptides / Q000378:metabolism*; D010957:Plasmids
chemical_list: D002352:Carrier Proteins; D002951:Citrates; D004274:DNA, Recombinant; D010455:Peptides; C020723:citrate-binding transport protein; D019343:Citric Acid; D004262:DNA Restriction Enzymes