Involvement of protease in L-glutamine control of nucleic acid and polyphosphate metabolism in cells transformed by 9,10-dimethyl-1,2-benzanthracene, SV40 and H-ras oncogene.
Mammalian cells transformed with either 9,10-dimethyl-1,2-benzanthracene, SV40 or H-ras oncogene dramatically changed their ability to synthesize DNA and RNA and metabolize polyphosphate when L-glutamine was withdrawn from the growth medium or when heat shocked (growth at 42 degrees C). Untransformed, DNA and RNA synthesis decreased by 50-80% when glutamine was withdrawn, but polyphosphate accumulated whether or not glutamine was supplied. Heat shock did not alter this response. Transformed isogenic cells responded differently; at 37 degrees C, they decreased their synthesis of DNA and RNA if starved for glutamine, whereas at 42 degrees C, synthesis was optimal without glutamine. Transformed cells accumulated polyphosphate at 37 degrees C when starved for glutamine, but at 42 degrees C, no polyphosphate accumulated. This apparent non-dependence on glutamine by transformed cells when heat shocked was found to be due to the production of glutamine from serum proteins through induction of a protease(s).
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004261:DNA Replication / Q000187:drug effects; D005973:Glutamine / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008168:Lung; D009857:Oncogenes; D010447:Peptide Hydrolases / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics*; D013696:Temperature; D014158:Transcription, Genetic / Q000187:drug effects
D011122:Polyphosphates; D011480:Protease Inhibitors; D005973:Glutamine; D015127:9,10-Dimethyl-1,2-benzanthracene; D010447:Peptide Hydrolases