Cyclic nucleotide metabolism in differentiated and undifferentiated epithelial thyroid cells in culture.
A highly differentiated thyroid cell line (FR-RL) was compared with a less differentiated (FR-T Cl1) and an undifferentiated (1-5G) cell line. FR-TL is modulated in vivo and in vitro by thyrotropin and has the lowest adenylate cyclase and guanylate cyclase and the highest phosphodiesterase activities. In contrast, 1-5G tumor cells do not respond to thyrotropin and have the highest adenylate cyclase guanylate cyclase and lowest hydrolyzing enzyme activities. Intermediate enzyme activities were found in FR-T Cl1 cells. The differences between the two normal rat thyroid cell lines are not due to differences in the composition of the growth medium.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D004848:Epithelium / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology; D013972:Thyrotropin / Q000494:pharmacology
D003470:Culture Media; D009712:Nucleotides, Cyclic; D013972:Thyrotropin; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase