title: Optimizing selection of restriction enzymes in the search for DNA variants.
abstract: A model is developed for predicting the relative efficiencies of different enzymes for detecting DNA variants when such variants are the result of single base-pair changes. 71 enzymes are analyzed for this ability in human DNA. Their relative ranked efficiencies are influenced by the sizes of the probes used, and the size of the smallest detectable fragment produced.
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D014644:Genetic Variation; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008957:Models, Genetic; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes