Antibodies against synthetic oligopeptides allow identification of the mRNA-maturase encoded by the second intron of the yeast cob-box gene.
Genetic and biochemical evidence has strongly suggested that several introns located in yeast mitochondrial genes specifying apocytochrome b or cytochrome oxidase encode trans-acting proteins (termed mRNA-maturases) responsible for splicing the cognate intron and maturation of the mRNA. We have chemically synthesized three oligopeptides, predicted from the DNA sequence of the open reading frame (ORF) present in the second intron of the cob-box gene, and raised antibodies against them. These antibodies have allowed us to identify a protein of 42 kd as the product translated from the ORF of the wild-type intron. In two splicing-deficient mutants this protein is replaced by shorter polypeptides whose lengths and antigenic properties are in full agreement with the positions of TAA codons established by the DNA sequence of the intron's ORF.
D000908:Antibodies, Fungal / Q000276:immunology; D001483:Base Sequence; D003062:Codon; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D005800:Genes, Fungal; D009713:Nucleotidyltransferases / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D009842:Oligopeptides / Q000138:chemical synthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D000908:Antibodies, Fungal; D003062:Codon; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D009842:Oligopeptides; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases