Characteristics and distribution of muscimol binding sites in cat visual cortex.
In vitro receptor binding techniques were used to study the characteristics and distribution of [3H]muscimol binding sites in cat visual cortex. [3H]muscimol, a specific GABA agonist, labeled a single population of binding sites with a Kd of 18 nM. Specific binding was saturable, reversible, and was blocked by the addition of GABA or (+)-bicuculline. Autoradiograms revealed that the highest density of [3H]muscimol binding sites occurred in cortical layer IV. Little variation between the various visual cortical areas was noted in contrast to marked regional heterogeneity within subcortical structures.
D000818:Animals; D001345:Autoradiography; D002415:Cats; D007555:Isoxazoles; D007700:Kinetics; D009118:Muscimol / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000378:metabolism*; D014018:Tissue Distribution; D014316:Tritium; D014793:Visual Cortex / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D007555:Isoxazoles; D010080:Oxazoles; D011963:Receptors, GABA-A; D014316:Tritium; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; C041650:isothaz