Peripheral-type benzodiazepine binding sites in a renal epithelial cell line (MDCK).
Madin-Darby canine kidney (MDCK) cells express a high density of binding sites (Bmax = 0.67 pmol/10(6) cells) for [3H]RO 5-4864, a peripheral-type benzodiazepine (BZD) receptor ligand. Receptor affinity (Kd = 45 nM) in MDCK cells is 30-50 fold lower than in rat kidney, but its pharmacological specificity is identical to that of peripheral-type BZD receptors in the rat kidney (PK 11195 greater than RO 5-4864 greater than diazepam = flunitrazepam greater than clonazepam). The MDCK cell line should provide a useful model system for studying the role of peripheral type BZD receptors in renal function.
D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003292:Convulsants / Q000378:metabolism; D004285:Dogs; D004848:Epithelium / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D011963:Receptors, GABA-A / Q000032:analysis* / Q000378:metabolism
D001570:Benzodiazepinones; D003292:Convulsants; D007473:Ion Channels; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam