title: Isolation and structure of a peptide hormone predicted from a mRNA sequence. A second somatostatin from the catfish pancreas.
abstract: This laboratory recently observed a cDNA reverse transcript to a specific mRNA from the channel catfish (Ictalurus punctata) pancreas which predicted the presence of a somatostatin gene product different from the previously reported catfish somatostatin (Oyama, H., Bradshaw, R. A., Bates, O. J., and Permutt, A. (1980) J. Biol. Chem. 255, 2251-2254). The gene product was purified using reverse phase high performance liquid chromatography. The peptide was shown to be structurally identical with somatostatin-14 from other species by tryptic mapping, amino acid composition, and sequence. The level of somatostatin-14 in the catfish endocrine pancreas was determined to be 104 micrograms of immunoreactive somatostatin/g of tissue. These observations suggest that the catfish pancreas has at least two structurally distinct somatostatins.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D005399:Fishes; D010179:Pancreas / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics*; D013004:Somatostatin / Q000235:genetics* / Q000302:isolation & purification; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D012333:RNA, Messenger; D013004:Somatostatin; D014357:Trypsin