Transglutaminase modifies the carboxy-terminal intracellular region of HLA-A and -B antigens.
The HLA-A and -B antigens, encoded in the major histocompatibility complex, are transmembrane proteins whose carboxy termini project inwards from the cell membrane. The intracellular region of the HLA-B7 antigen is known to include two glutamine residues. We have used guinea pig transglutaminase to show that in vitro the enzyme couples amines specifically to glutamine residues in the carboxy-terminal region of the HLA-B7 and -A2 antigens. The specificity of this reaction has an immediate practical significance, but may also have a wider biological interest.
D003593:Cytoplasm / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006680:HLA Antigens; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D006023:Glycoproteins; D006680:HLA Antigens; D008565:Membrane Proteins; D005723:gamma-Glutamyltransferase; D011700:Putrescine