A new fluorescence method for gamma-glutamyltransferase isoenzyme demonstration.
A new fluorescence method for demonstrating gamma-glutamyltransferase isoenzymes using gamma-glutamyl-5-amino-4-methyl coumarin, a commercially available, non-carcinogenic substrate is described. The procedure can readily detect gamma-glutamyltransferase isoenzymes in samples with total activity as low as 5 U/l at 30 degrees C.
D005453:Fluorescence; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D005723:gamma-Glutamyltransferase / Q000097:blood*
D007527:Isoenzymes; D005723:gamma-Glutamyltransferase