[Infectious bovine rhinotracheitis (VHB-1). II. Physico-chemical properties of strain L-114].
The thermal stability, density gradient and morphological features of a recently isolated L-114 strain of infectious bovine rhinotracheitis virus were determined. Morphology studies showed an enveloped virus with 200 nm of total diameter, a core diameter of 90 nm and an icosaedral-type structure; purified preparations contained both complete and empty viral particles. More than 90% of the infectivity was lost after 15 hours at 37 degrees C; at 56 degrees C, inactivation was much faster, with a 3 log titer reduction, in 24 minutes. Density gradient studies in cesium chloride, carried out with virus concentrated on sucrose gradient, gave an estimated density of 1.25 g/ml for the purified virus, which fits with light herpesviruses.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007242:Herpesvirus 1, Bovine / Q000648:ultrastructure*; D013696:Temperature
Rinotraqueítis infecciosa bovina (VHB-1). II: Propiedades físico-químicas de la cepa L-114.