title: Phenotype characteristics of human B cells studied by Epstein-Barr virus infection. III. In vitro modulation of C3 receptor expression on human lymphoid cells.
abstract: An addition of dimethyl sulfoxide (DMSO) in culture medium enhanced the expression of receptor (R) for EACh (C3b-R) of the lymphoblastoid cells derived from immature B cells which were expressing little or no EACh-R. EACm-R (C3bi- and C3d-R) on the lymphoblastoid cells of bone marrow cells decreased remarkably (25 to 5%) during short term cultivation, though other markers remained unchanged. In addition, C3-R expression of the lymphoblastoid cell lines from bone marrow cells, which were established by EBV infection before and after 4-day cultivation, was quite similar to that of the cell of origin. These results, in accordance with our previous paper (Kudo, T. and Tachibana, T. (1984) Immunol. Lett. 8,343-348), support the idea that the sequential switching of C3 receptors, EACm-R----EACh-R, occurs during B cell differentiation.
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004854:Herpesvirus 4, Human; D006801:Humans; D016177:Macrophage-1 Antigen; D010641:Phenotype; D011951:Receptors, Complement / Q000187:drug effects
chemical_list: D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D004121:Dimethyl Sulfoxide