Increasing membrane polyunsaturated fatty-acid content augments cyclic AMP formation and prostaglandin production in NIE-115 neuroblastoma.
Addition of linoleic acid (50 microM) to culture medium significantly increases levels of polyunsaturated fatty acids (PUFA) in membrane phospholipids of NIE-115 neuroblastoma. Basal levels of cyclic AMP are elevated significantly in supplemented cells. Exogenous prostaglandins (PG) PGE1 and PGD2 stimulate cAMP formation in NIE-115 neuroblastoma. Supplemented cells produce higher levels of PGE and PGD than do control cultures. Inclusion of cyclooxygenase inhibitors in culture medium does not block elevation of cyclic nucleotide in supplemented cells. Endogenous PG production and receptor activation cannot account for increased cAMP in EFA-supplemented neuroblastoma.
D000818:Animals; D002999:Clone Cells; D000242:Cyclic AMP / Q000096:biosynthesis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000502:physiology*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008563:Membrane Lipids / Q000032:analysis / Q000502:physiology*; D051379:Mice; D009447:Neuroblastoma; D011453:Prostaglandins / Q000096:biosynthesis*
D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008563:Membrane Lipids; D011453:Prostaglandins; D019787:Linoleic Acid; D000242:Cyclic AMP