title: Formation of covalently closed heteroduplex DNA by the combined action of gyrase and RecA protein.
abstract: The concerted action of DNA gyrase and RecA protein of Escherichia coli on intact and gapped homologous or partially homologous plasmid DNA molecules leads to the formation of covalently closed DNA containing one strand of each parental molecule. Large regions of non-homology can be incorporated into the closed circular duplex. Both proteins are essential for the reaction to take place, and type I topoisomerase cannot substitute for DNA gyrase.
mesh_terms: D001483:Base Sequence; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D009692:Nucleic Acid Heteroduplexes / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids; D011938:Rec A Recombinases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009692:Nucleic Acid Heteroduplexes; D011938:Rec A Recombinases; D004250:DNA Topoisomerases, Type II