Crystallization of intrinsic membrane proteins.
After many years of discouraging failures, it is now possible to crystallize the intrinsic membrane proteins and to obtain structural information from diffraction studies on the crystals. The strategy for the crystallization consists of depletion of boundary phospholipids from the protein and complex formation with specific ligands.
D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D001436:Bacteriorhodopsins; D002308:Cardiolipins; D003461:Crystallography; D003580:Cytochromes; D003576:Electron Transport Complex IV; D005614:Freeze Fracturing; D008565:Membrane Proteins; D010743:Phospholipids; D018272:Porins; D011487:Protein Conformation; D014961:X-Ray Diffraction
D001425:Bacterial Outer Membrane Proteins; D002308:Cardiolipins; D003580:Cytochromes; D008565:Membrane Proteins; D010743:Phospholipids; D018272:Porins; D001436:Bacteriorhodopsins; D003576:Electron Transport Complex IV