Construction and properties of plasmid pKC30, a pBR322 derivative containing the pL-N region of phage lambda.
The phage lambda N gene, contained on a HindIII-BamHI lambda DNA fragment (lambda coordinates 38 695-34 500), was inserted into plasmid pBR322 and cloned in Escherichia coli containing a defective lambda prophage. The plasmid is called pKC30. lambda N-mediated pL transcription interfered with plasmid pKC30 maintenance. Plasmid pKC30 was stabilized (i) by repression of pL, (ii) by a nutL mutation, or (iii) by cloning an "N-unresponsive terminator" sequence downstream from N gene.
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins