Functional characteristics of L-glutamate, N-methyl-D-aspartate and kainate receptors in isolated brain synaptic membranes.
L-Glutamic acid (L-Glu) and other excitatory amino acids and amino acid analogs enhanced [35S]thiocyanate (SCN-) uptake in isolated-resealed synaptic membrane vesicles. The SCN- uptake was used as a measure of membrane depolarization to evaluate the characteristics of functional excitatory amino acid receptors in the synaptic membranes. N-Methyl-D-aspartate (NMDA) and L-Glu produced additive effects on SCN- accumulation indicating the presence of distinct L-Glu and NMDA receptors. On the other hand, kainic acid (KA) and L-Glu shared either common receptor sites or ion channels. The effects of antagonists on NMDA, L-Glu, and KA stimulation of SCN- influx were consistent with previously reported electrophysiologic observations in intact neurons.
D000818:Animals; D001921:Brain / Q000502:physiology*; D002463:Cell Membrane Permeability; D008297:Male; D008564:Membrane Potentials; D051381:Rats; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D013570:Synaptic Membranes / Q000502:physiology*; D013861:Thiocyanates / Q000378:metabolism
D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D013861:Thiocyanates; C031760:thiocyanate