[Multiplication of Aujeszky's disease virus MK-25 on chick fibroblast cell cultures].
Attempts were made to produce multilayer cell cultures of chick fibroblasts in a RC-42 roller as well as to replicate in these the Aujezsky's disease virus. Used were fibroblasts of 10-11-day-old chick embryos. The cells were obtained in a closed system after the classic method with an electromagnetic stirrer and a 0.3 percent trypsin in phosphate buffer, having no Ca and Mg ions. Hank's solution was used as a nutrient growth medium, containing 0.5 per cent lactoalbumin hydrolysate and calf serum at pH 7.4-7.6. Both the cell growth and the cytopathic effect of the virus in the stationary and the roller cultures were carried out simultaneously through microscopy, and the evaluation was made after a fourgrade system. It was found that proper cell cultures or chick fibroblasts could be obtained under the following optimal parameters: volume of nutrient medium--200 cm.3; amount of normal inactivated calf sera--10 per cent; and speed of rotation--10 r. p. m. The titer of the virus was found to surpass by 2 logarithms the virus in the stationary cell cultures for a twice shorter period of cultivation.
D000818:Animals; D002642:Chick Embryo; D005347:Fibroblasts / Q000382:microbiology; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D014776:Virus Cultivation / Q000295:instrumentation / Q000379:methods; D014779:Virus Replication
Namnozhavane na virusa na bolestta na Aueski MK-25 vŭrkhu kletŭchni kulturi ot pileshki fibroblasti.