[In vitro culture of clonogenic cells from various human tumors: chemosensitivity tests].
One hundred and thirty three specimens from mammary and ovarian adenocarcinoma and from melanoma were cultured according to an agar/agar clonogenic assay. Melanoma and ovarian cancers exhibited a 70 per cent rate of success for culture; 50 per cent of the mammary adenocarcinomas were successfully cultured. Fifty-nine ovarian cancers were cultured in order to test the in vitro effectiveness of Cisplatinum and Adriamycin. Thirty percent of cultured tumors gave rise to relevant chemograms. The chemoresistance measured in vitro was correlated to the ineffectiveness of the patient's treatment. In contrast, we were unable to predict chemosensitivity. Taking into account the technical difficulties encountered in these assays, human tumor clonogenic assays cannot at present be proposed as a routine procedure in the prediction of the effectiveness of chemotherapeutic treatments. Nevertheless, they must be developed in order to determine the spectrum of activity of new antineoplastic agents on various human tumors.
D000230:Adenocarcinoma / Q000188:drug therapy; D000362:Agar; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000188:drug therapy; D002945:Cisplatin / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D003520:Cyclophosphamide / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance; D005260:Female; D006801:Humans; D008545:Melanoma / Q000188:drug therapy; D008558:Melphalan / Q000494:pharmacology; D010051:Ovarian Neoplasms / Q000188:drug therapy; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents; D004317:Doxorubicin; D003520:Cyclophosphamide; D000362:Agar; D002945:Cisplatin; D008558:Melphalan
Culture in vitro des cellules clonogènes de différentes tumeurs humaines: essais de chimiosensibilité.