title: F plasmid pif region: Tn1725 mutagenesis and polypeptide analysis.
abstract: Analysis of Tn1725 insertions in the Pif+ plasmid pRS2496 showed the maximum limits of the F pif region to be between 43.7 and 47.15 on the 100-kb map of the F plasmid. The effect of these insertions on the expression of pif polypeptides indicated that two of the pif genes, pifA and pifC, lie within a polycistronic operon.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005786:Gene Expression Regulation; D009154:Mutation; D009876:Operon; D010455:Peptides / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010455:Peptides; D012097:Repressor Proteins