Resistance of Semliki forest virus protein synthesis to high salt treatment.
Selective translation of Semliki Forest virus-specific mRNA occurred in virus-infected cells exposed to hypertonic growth medium. The selective resistance of the virus-specific protein synthesis could be detected at a wide NaCl concentration range and was more significant at lowered incubation temperature (28 degrees C). It is suggested that the translation of the structural proteins encoding subgenomic 26 S RNA is more resistant to the hypertonic initiation block than the translation of the genomic 42 S RNA which codes for the non-structural viral proteins.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012672:Semliki forest virus / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis*
D003470:Culture Media; D012333:RNA, Messenger; D012367:RNA, Viral; D012462:Saline Solution, Hypertonic; D014764:Viral Proteins; D012965:Sodium Chloride