[Study of the secondary and tertiary structure of phage MS2 RNA using nucleases and fluorescent dyes specific for secondary structure].
The binding of ethidium bromide (EtBr) and acridine orange (AO) to RNA in native state or after hydrolysis by S1 and SV nucleases that specifically split single-stranded and double-stranded segments was studied. Nuclease S1 hydrolysis of RNA does not increase the number of EtBr strong binding sites, Tm and hyperchromic effect being also unchanged. Hydrolysis by double-stranded segments accessible to EtBr is followed by the diminishing of Tm and hyperchromism. A supposition is put forward that the main role in stabilization of the RNA tertiary structure is played by double-stranded segments arranged so that some of them are hidden and do not interact with dyes. One of the possible models may be parallel oriented intramolecular "hair-pins" forming compact "rod-like" structures.
D003090:Coliphages; D004720:Endonucleases; D004722:Endoribonucleases; D005456:Fluorescent Dyes; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D015719:Single-Strand Specific DNA and RNA Endonucleases
D005456:Fluorescent Dyes; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D004720:Endonucleases; D004722:Endoribonucleases; C043004:cobra venom endonuclease; D015719:Single-Strand Specific DNA and RNA Endonucleases
Izuchenie vtorichnoĭ i tretichnoĭ struktury RNK faga MS2 s ispol'zovaniem nukleaz i fluorestsiruiushchikh krasiteleĭ, spetsifichnykh ko vtorichnoĭ strukture.