Analysis of benzodiazepine binding sites in rat pituitary gland.
Benzodiazepine binding sites in rat pituitary gland were investigated by equilibrium binding assays in vitro. Using [3H]flunitrazepam [( 3H]FNM), specific binding was demonstrated in both anterior and neurointermediate lobes. Clonazepam and Ro 5-4864 were used to discriminate the binding to central and peripheral-type sites. Both types of binding site were found to be relatively concentrated in the neurointermediate lobe. Scatchard analysis demonstrated that Kd values for [3H]FNM for the two types of sites were similar to those reported in other areas.
D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D001667:Binding, Competitive; D002998:Clonazepam / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism; D005445:Flunitrazepam / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D001570:Benzodiazepinones; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D005680:gamma-Aminobutyric Acid; D002998:Clonazepam; D005445:Flunitrazepam; D003975:Diazepam