title: Continuous culture of Reuber hepatoma cells in serum-free arginine-, glutamine- and tyrosine-deprived chemically defined medium.
abstract: The rat hepatoma cell line, H4-II-E, was grown serially over a 1-year period and about 30 passages in arginine-, glutamine-, and tyrosine-deprived and ornithine-supplemented Eagle's minimum essential medium with no supplements other than biotin. The adapted cell line, R-Y121B, proliferates in the above mentioned medium with a doubling time of about 4 days and maintains hepatic "marker" enzymes such as tyrosine aminotransferase, phenylalanine hydroxylase, and all the enzymes of the urea cycle.
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D001123:Argininosuccinate Lyase / Q000378:metabolism; D001124:Argininosuccinate Synthase / Q000378:metabolism; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000378:metabolism; D003470:Culture Media; D005973:Glutamine / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology*; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D010651:Phenylalanine Hydroxylase / Q000378:metabolism; D051381:Rats; D014443:Tyrosine / Q000378:metabolism; D014444:Tyrosine Transaminase / Q000378:metabolism
chemical_list: D003470:Culture Media; D005973:Glutamine; D014443:Tyrosine; D001120:Arginine; D010651:Phenylalanine Hydroxylase; D009954:Ornithine Carbamoyltransferase; D014444:Tyrosine Transaminase; D001119:Arginase; D001123:Argininosuccinate Lyase; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D001124:Argininosuccinate Synthase