The relevance of some laboratory tests in the diagnosis of cholestasis and the differentiation between intra- and extrahepatic obstruction.
The Lp-X test and the determination of total gammaglutamyltransferase activity (GGT), its isoenzyme activities (GGT-1 and GGT-2) and the bile acids have been performed in sera obtained from 66 patients with and without cholestasis. The sensitivity of the Lp-X test as an indicator for cholestasis appeared to be 89% with a specificity of 94%. With the exception of GGT-2 activity, the mean values of total GGT, GGT-1 isoenzyme and bile acids differed significantly between the cholestasis positive and negative groups. The diagnostic use of total GGT, isoenzymes and bile acids in the differentiation between intrahepatic cholestasis and extrahepatic biliary obstruction has been investigated. The GGT-1 isoenzyme activity appeared to be the only parameter differing significantly between the two forms of cholestasis.
D001647:Bile Acids and Salts / Q000097:blood; D001651:Cholestasis, Extrahepatic / Q000175:diagnosis*; D002780:Cholestasis, Intrahepatic / Q000175:diagnosis*; D019411:Clinical Laboratory Techniques; D003937:Diagnosis, Differential; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008073:Lipoprotein-X / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
D001647:Bile Acids and Salts; D007527:Isoenzymes; D008073:Lipoprotein-X; D005723:gamma-Glutamyltransferase