The influence of dietary unsaturated cis and trans and saturated fatty acids on tissue lipids of swine.
A feeding trial was conducted to evaluate the effects of dietary trans unsaturated fatty acids (trans fat) and of the interplay of dietary saturated fatty acids (saturated fat), cis unsaturated fatty acids, (cis fat) and trans fat on tissue lipids, particularly those effects suggestive of angiotoxicity. Swine were fed for 10 months a diet containing 17% added fat. Seven blends of varying proportions of the 3 fat components provided sufficient sample points to permit an examination of the interplay. Parameters under study included weight gain, serum cholesterol and triglyceride concentrations, lipoprotein lipid profile, total lipid and cholesterol concentrations of liver, heart and aorta, fatty acid composition of liver and aorta lipids and hepatic fatty acid synthesis and cholesterol synthesis and oxidation. Fat blends containing disproportionately high levels of saturated or cis fat generally elicited responses consistent with results reported by others. The notable exception was the serum cholesterol concentration. Throughout the study, the swine were hypercholesterolemic. Swine fed the high saturated fat blend had serum cholesterol levels equal to those swine fed the high cis fat blend. Serum cholesterol levels in the swine fed the other fat blends were more elevated. Another apparent anomaly was the lower concentration of lipid in the aortas of swine fed the high-saturated fat diet. The impact of the trans fat was modulated by the relative proportions of saturated and cis fat in the diet. The impact of trans fat was of greater magnitude for most parameters when the fat blend was low in saturated fat. The sole parameter suggestive of trans fat-mediated angiotoxicity was the distribution of lipids in lipoprotein fractions. Swine fed diets containing trans fat had lower relative proportions of the alpha-lipoprotein lipids. Although hypercholesterolemic, the high fat diets were not overtly angiotoxic except when fed to swine that carried a specific immunogenetically-defined low density lipoprotein.
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D007536:Isomerism; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D013552:Swine
D002788:Cholesterol Esters; D004041:Dietary Fats; D005227:Fatty Acids; D002784:Cholesterol; D000103:Acetyl-CoA Carboxylase