Hemoglobin Miyada: DNA analysis of the anti-Lepore beta delta fusion gene.
Hemoglobin Miyada, an anti-Lepore hemoglobin, represents the protein product of a nonhomologous crossover between beta and delta genes. The mutant globin is beta-like from the N terminus to amino acid 12, and delta-like from amino acid 22 through the C terminus, thus predicting a crossover site in the first coding region of the gene. DNA analysis, using multiple restriction endonucleases and hybridization to delta and beta globin gene-specific probes, confirms that the beta delta hybrid gene 1) is located on a single chromosomal fragment between normal delta and beta genes, and 2) has 5' beta promoter sequences, delta IVS 1 and 2, and 3' coding and flanking sequences.
D001483:Base Sequence; D003434:Crossing Over, Genetic; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D005796:Genes; D005914:Globins / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000235:genetics*; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification; D011401:Promoter Regions, Genetic
D004251:DNA Transposable Elements; D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments; D005914:Globins; D004247:DNA; C040869:hemoglobin Miyada; C037659:hemoglobin Lepore; D004262:DNA Restriction Enzymes