Coxsackie-B-virus-specific IgM responses in patients with cardiac and other diseases.
An enzyme-linked immunosorbent assay (ELISA) test using polyvalent antigens and antisera was developed to detect Coxsackie-B-virus-specific IgM responses. The sera of 24 of 64 (37.5%) patients with acute pericarditis and 14 of 38 (36%) with acute myocarditis were positive for Coxsackie-B-virus-specific IgM. 4 of 30 (13.3%) patients with acute ischaemic heart disease and 2 of 28 (7.1%) patients with congestive cardiomyopathy were also positive. Coxsackie-B-virus-specific IgM was detected in the sera of 21 of 57 (36.8%) patients with Bornholm disease and 2 of 4 patients with acute-onset juvenile diabetes. Coxsackie-B-virus-specific IgM responses persisted for 6-8 weeks. Sera from patients with chronic valvular heart disease, Mycoplasma pneumoniae infections, and virus infections caused by viruses other than Coxsackie-B viruses were all negative. False-positive results did not occur when sera containing high titres of rheumatoid factor were tested.
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology; D029822:Enterovirus B, Human / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006331:Heart Diseases / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D008582:Meningitis, Aseptic / Q000276:immunology; D008875:Middle Aged; D009205:Myocarditis / Q000276:immunology; D010493:Pericarditis / Q000276:immunology; D011000:Pleurodynia, Epidemic / Q000276:immunology; D013045:Species Specificity
D000914:Antibodies, Viral; D007075:Immunoglobulin M