Antibodies to a chemically synthesized peptide which precipitate a v-myb protein.
A synthetic peptide with a composition derived from the DNA sequence of v-myb of AMV was used as a hapten for immunization of rabbits. Peptide-specific immunoglobulins were isolated by affinity chromatography on a peptide-Sepharose column and used for immuno-precipitation of lysates of AMV-transformed non-producer myeloblasts. Two other proteins with molecular weights of 30 000 and 56 000 daltons apparently not related to myb protein were specifically coprecipitated with p48v-myb.
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D001354:Avian Leukosis Virus / Q000276:immunology*; D009189:Avian Myeloblastosis Virus / Q000276:immunology*; D006241:Haptens; D007074:Immunoglobulin G / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D006241:Haptens; D007074:Immunoglobulin G; D010455:Peptides; D014764:Viral Proteins