A new fluorimetric assay for streptothricins.
In strongly acidic medium (70% HClO4) streptothricins form a fluorophore (lambda ex = 312 nm; lambda em = 381 nm) with unknown structure. A fluorimetric determination of pure or crude products and cultures, respectively, was worked out based on this reaction. Concentrations for fluorescence measurements were in the range of 10(-8) - 2 X 10(-7) moles. Interferences of the assay are discussed, a statistical evaluation of results and a comparison between microbiological and fluorimetric findings are given.
D000900:Anti-Bacterial Agents / Q000032:analysis*; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D002854:Chromatography, Paper; D005470:Fluorometry / Q000379:methods*; D006851:Hydrochloric Acid; D006868:Hydrolysis; D013309:Streptothricins / Q000032:analysis* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D013309:Streptothricins; D006851:Hydrochloric Acid