Skeletal muscle fibers in suspension: a new approach to the study of oxidative and glycolytic metabolism in differentiated muscle.
A preparation of suspended fibers from m. flexor digitorum brevis of the rat was characterized with respect to morphological features, and its relevance for the study of muscular metabolism investigated. The activities of oxidative (palmitate and pyruvate oxidation) and glycolytic (lactate formation) pathways were enhanced in myofiber suspensions when compared to intact whole muscle. The rate of glycolysis was stimulated about two-fold by insulin in both the myofiber suspensions and intact muscle. Parameters of oxidative metabolism responded similarly to metabolic effectors in the myofiber suspensions and in intact muscle. It is concluded that the myofiber suspensions have distinct advantages over intact muscle for biochemical and pharmacological studies.
D000818:Animals; D002331:Carnitine / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D003999:Dichloroacetic Acid / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology; D027501:Monocarboxylic Acid Transporters; D009132:Muscles / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002934:Cinnamates; D007328:Insulin; D007773:Lactates; D027501:Monocarboxylic Acid Transporters; D010168:Palmitates; D010169:Palmitic Acids; D011773:Pyruvates; C018253:alpha-cyanocinnamate; D003999:Dichloroacetic Acid; D003012:Microbial Collagenase; D005947:Glucose; D002331:Carnitine