title: Rapid polyphosphoinositide decrease is an early event in the steroidogenic response of bovine adrenocortical fasciculata cells to angiotensin II.
abstract: Addition of angiotensin II (0.3 microM) to bovine adrenal fasciculata cell suspensions prelabeled with [32P] induced a rapid (15 seconds) and marked decrease of the radioactivity from phosphatidylinositol 4,5-biphosphate (62%) and phosphatidylinositol 4-monophosphate (35%). This effect was concentration-dependent and specifically inhibited in the presence of (Sar1-Ala8)-angiotensin II; it was also completely prevented in the absence of extracellular calcium. The present data appear to illustrate the earliest biological response detectable in bovine fasciculata cells under angiotensin II challenge.
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D066298:In Vitro Techniques; D007700:Kinetics; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D010761:Phosphorus Radioisotopes
chemical_list: D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D000804:Angiotensin II