Effect of LiCl on differentiation of mouse embryos beyond the blastocyst stage.
In order to test the effect of Li+ on cell differentiation, 2-cell embryos were cultured in vitro with or without Li+ to the blastocyst stage and then, in a "implantation" culture medium, with or without Li+, up the early egg-cylinder stage. Our results show: 1) that Li+ in the preimplantation medium diminishes "implantation" success significantly; 2) that trophoblastic vesicles, produced by culturing preimplantation embryos with Li+, do not generate an inner cell mass when further cultured in "implantation" medium; 3) that the ultrastructure of "implanted" embryos is similar in all experimental series.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000648:ultrastructure; D002454:Cell Differentiation / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D003470:Culture Media; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D008094:Lithium / Q000494:pharmacology*; D018021:Lithium Chloride; D051379:Mice; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011247:Pregnancy
D002712:Chlorides; D003470:Culture Media; D008094:Lithium; D018021:Lithium Chloride