Mechanism of illegitimate recombination: common sites for recombination and cleavage mediated by E. coli DNA gyrase.
Illegitimate recombination dependent on DNA gyrase in a cell-free system has previously been described. We have now mapped DNA gyrase cleavage sites in the vicinity of known recombination sites in pBR322. Among five recombination sites examined, three were found to coincide with a DNA gyrase cleavage site. This result suggests that the cleavage of DNA by DNA gyrase has a central role in the recombination process.
D001483:Base Sequence; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004274:DNA, Recombinant / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic
D004274:DNA, Recombinant; D004250:DNA Topoisomerases, Type II