Use of reflection contrast microscopy in the analysis of cellular motility.
Recognition and determination of the following activities of living cancer cells on glass substrates can be greatly facilitated by the use of reflection contrast microscopy: 1. stationary versus translocative motility, 2. migration over/under other cells, 3. actual locomotory activity of cells with a polarized shape usually associated with this type of motility. In addition, reflection contrast is useful for recognizing the presence of fibroblasts in cancer cell populations.
D000818:Animals; D002465:Cell Movement; D005347:Fibroblasts; D008853:Microscopy / Q000379:methods*; D009369:Neoplasms / Q000473:pathology; D011817:Rabbits