[Isolation and characteristics of IgA1 and its use for detecting bacterial IgA1 proteases].
Sufficiently purified IgA, subclass I, has been isolated from the defibrinated plasma of a myeloma patient by chromatography on columns packed with DEAE-Sephadex A-50 or Sephadex G-200, and rabbit antiserum to this immunoglobulin has been obtained. These preparations have been used for detecting specific protease in Bordetella pertussis. The tested B. pertussis strains have been shown to induce, as revealed by immunoelectrophoretic methods, the proteolysis of human IgA, subclass I.
D000818:Animals; D001886:Bordetella pertussis / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification*; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000276:immunology; D010447:Peptide Hydrolases / Q000032:analysis* / Q000302:isolation & purification; D011817:Rabbits; D012697:Serine Endopeptidases; D012756:Sheep / Q000276:immunology
D007070:Immunoglobulin A; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase
Vydelenie,i kharakteristika IgA1 i ego ispol'zovanie dlia obnaruzheniia bakterial'nykh IgA1-proteaz.