Translation of genomic avian myeloblastosis virus RNA in a cell-free protein synthesis system from rabbit reticulocytes.
Reaction conditions suitable for translation of genomic avian myeloblastosis virus RNA in micrococcal nuclease-pretreated reticulocyte lysates are described. The products of translation were characterized by immunoprecipitation and gel electrophoresis and compared with virus-specific products formed in host cells. Genomic viral RNA directed in a cell-free system the synthesis of precursors to viral structural proteins, namely Pr76gag and Pr180gag,pol.
D000818:Animals; D001354:Avian Leukosis Virus / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000032:analysis / Q000378:metabolism*; D002474:Cell-Free System; D010455:Peptides / Q000032:analysis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins