Duplicated heavy metal control sequences of the mouse metallothionein-I gene.
We present evidence that two distinct regions of the DNA upstream from the mouse metallothionein-I gene contain metal-responsive regulatory sites. This result was obtained by analyzing a systematic series of deletion, insertion, duplication, and clustered point mutations introduced into cultured cells on a simian virus 40 plasmid vector. The two upstream regions contain a duplicated evolutionarily conserved DNA sequence. While either upstream region is sufficient to confer heavy metal responsiveness, both are required to give maximal levels of induced transcription.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D007668:Kidney; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D008670:Metals / Q000494:pharmacology*; D051379:Mice; D009154:Mutation; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D008670:Metals; D008668:Metallothionein; D004262:DNA Restriction Enzymes