Polypeptides encoded by polyadenylated and non-polyadenylated messenger RNAs from normal and heat shocked HeLa cells.
There is an increased synthesis of proteins in the molecular weight region of 100,000 72,000-74,000 and 37,000 two hours after treatment of HeLa cells for 10 min at 45 degrees C. In vitro translation, using a rabbit reticulocyte cell-free protein synthesising system, of HeLa cell cytoplasmic RNA shows that the prominent 72,000-74,000 Mr heat shock protein band comprises seven polypeptide species (namely alpha d beta gamma delta epsilon zeta) and these polypeptides are directly encoded by both polyadenylated and nonpolyadenylated mRNA.
D000818:Animals; D006367:HeLa Cells / Q000378:metabolism; D006360:Heat-Shock Proteins; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics*; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D006360:Heat-Shock Proteins; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; D011061:Poly A