title: Cloning of genomic DNA for human atrial natriuretic factor.
abstract: A human genomic DNA clone for atrial natriuretic factor (ANF) gene was isolated from a human gene library using the previously cloned cDNA for rat ANF as a probe. Partial nucleotide sequencing of the cloned DNA revealed the location of a 120-bp long intron between Lys-41 and Asn-42 of the ANF precursor. Restriction mapping also suggested the existence of at least one other intron in or proximal to the 3' -untranslated region.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor; D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005260:Female; D005333:Fetus; D005796:Genes; D006801:Humans; D008099:Liver / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D051381:Rats; D013045:Species Specificity
chemical_list: D009124:Muscle Proteins; C472955:NPPA protein, human; D009320:Atrial Natriuretic Factor; D004247:DNA; D004262:DNA Restriction Enzymes