A method for the measurement and characterization of protease activities responsible for extracellular or intracellular degradation of collagen precursors.
The first method for the qualitative and quantitative evaluation of extracellular and intracellular protease activities responsible for degradation of newly synthesized collagen is described. In a double incubation method, underhydroxylated collagen chains (protocollagen) serve as substrate for protease extract and then for the indicator enzyme, 4 prolyl hydroxylase. It was possible to characterize at least four types of protocollagen sites sensible to these proteases. The microsomal fraction of chick embryo liver contained a protease active on protocollagen and whose activity was similar to that of purified human synovial collagenase.
D000818:Animals; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D003012:Microbial Collagenase / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism*; D011347:Procollagen / Q000378:metabolism*; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D013552:Swine; D013917:Thrombin / Q000378:metabolism; D013977:Tibia / Q000201:enzymology; D014316:Tritium; D014357:Trypsin / Q000378:metabolism
D011347:Procollagen; D014316:Tritium; D003094:Collagen; D011393:Procollagen-Proline Dioxygenase; D010447:Peptide Hydrolases; D014357:Trypsin; D013917:Thrombin; D003012:Microbial Collagenase