title: Association of guanylate cyclase with the axoneme of retinal rods.
abstract: Axonemes were isolated from purified bovine retinal rod outer segments by dissolving the outer segment membranes in detergent and separating the axonemes by centrifugation on a linear detergent-containing sucrose density gradient. Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing), EC 4.61.2) activity was concentrated in the axoneme fraction. Guanylate cyclase eluted in the void volume when detergent-solubilized rod outer segments were subjected to exclusion chromatography on Sepharose 4B. Attempts to extract guanylate cyclase from isolated axonemes with salt, EDTA, base and other reagents were successful.
mesh_terms: D000818:Animals; D002417:Cattle; D006162:Guanylate Cyclase / Q000302:isolation & purification*; D010786:Photoreceptor Cells / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D006162:Guanylate Cyclase