title: A cauliflower mosaic virus promoter directs expression of kanamycin resistance in morphogenic transformed plant cells.
abstract: The promoter region of the CaMV inclusion body protein gene was modified for use in chimeric gene fusions. The modified promoter was used to construct a selectable marker for plant transformation based on the Tn 5 kanamycin resistance gene. This chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of Agrobacterium tumefaciens. Both types of transformation produced kanamycin-resistant cell lines. The resistant cell lines derived from the deoncogenized strains were used to regenerate shoots. A second type of selection based on the ability of octopine synthase to detoxify aminoethyl cysteine was also used to select transformants in both oncogenic and nononcogenic transformation.
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004351:Drug Resistance; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D007612:Kanamycin / Q000494:pharmacology; D019868:Kanamycin Kinase; D009029:Mosaic Viruses / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics; D010944:Plants / Q000235:genetics; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004279:DNA, Viral; D007612:Kanamycin; D000594:Amino Acid Oxidoreductases; C021903:D-octopine dehydrogenase; D010770:Phosphotransferases; D019868:Kanamycin Kinase