The stimulatory effect of platelet homogenate on prolyl hydroxylase activity in L-929 cells.
We have found that the addition of platelet homogenate to confluent cultures of L-929 cells increases 2-3 times the activity of prolyl hydroxylase in these cells. Furthermore, it was found that the platelet homogenate potentiates the effect of ferrous ions and ascorbic acid, which are known activators of prolyl hydroxylase. The effect of the platelet homogenate is diminished by cycloheximide. It seems probable that some products present in the platelet homogenate may promote biosynthesis of the enzyme or they stimulate glycolysis and accumulation of lactic acid, an activator of the hydroxylase.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D002417:Cattle; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005296:Ferrous Compounds / Q000494:pharmacology; D051379:Mice; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology
D005296:Ferrous Compounds; D000644:Quaternary Ammonium Compounds; D003094:Collagen; D003513:Cycloheximide; C038178:ammonium ferrous sulfate; D011393:Procollagen-Proline Dioxygenase; D001205:Ascorbic Acid