[Effects of various agents on the proliferation and hCG secretion of choriocarcinoma cells in vitro].
Effects of sex steroids, LH-RH, cyclic AMP and differentiation promoters on the proliferation and hCG secretion of two choriocarcinoma cell lines (BeWo and HCCM-5) were examined in vitro. The results were as follows. 1) Physiological concentrations of estradiol or progesterone revealed no effect on proliferation or hCG secretion, but pharmacological ones (more than 10 micrograms/ml) inhibited both the cell proliferation and its hCG secretion. 2) LH-RH and cyclic AMP slightly inhibited the proliferation of cells, and hCG secretion per cell was concomitantly enhanced. Cyclic AMP enhanced hCG secretion more markedly than LH-RH. 3) Retinoic acid had no effect on either cell proliferation or hCG secretion, whereas sodium butyrate inhibited both cell proliferation and hCG secretion. These results suggest that modifiers of hCG secretion may be grouped as follows; agents that inhibited cell proliferation slightly and enhanced hCG secretion markedly (LH-RH, cyclic AMP), agents that inhibited cell proliferation markedly and enhanced hCG secretion slightly (anticancer drugs) and agents that inhibited both cell proliferation and hCG secretion (estradiol, progesterone and sodium butyrate).
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology*; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
D006063:Chorionic Gonadotropin; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D000242:Cyclic AMP