title: In vitro adhesion of uropathogenic Escherichia coli to human periurethral cells.
abstract: The in vitro adhesion of three uropathogenic strains of Escherichia coli to epithelial cells from the periurethral area (area surrounding the urethral orifice) of women with and without a history of recurrent urinary tract infections was investigated. All strains showed a specific mannose-resistant hemagglutination restricted to human erythrocytes. Since only a few hundred periurethral cells were used in each test, gentle methods were required. Optimal results were obtained with bacteria grown for 16 h at 37 degrees C in nutrient broth without shaking. The binding of bacteria seemed to be irreversible under the conditions studied, since repeated washings of the epithelial cells after incubation did not decrease the number of adhering bacteria. Chloramphenicol was used to control the number of added bacteria in the incubation system. A difference in the adhesive capacity of periurethral cells of infection-prone and healthy individuals was most evident at concentrations of 2.5 x 10(9) bacteria/ml. Electron microscope studies indicated that pili mediated the adhesion. Adhesion was correlated with the mannose-resistant hemagglutination of human erythrocytes, indicating that the pili were not type 1 pili. Day-to-day variations in the adhesiveness of the bacteria were reduced by selecting well-adhering bacteria with the aid of in vitro passage on periurethral cells or human erythrocytes, and by exclusion of bacteria with low hemagglutination ability.
mesh_terms: D002470:Cell Survival; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology; D006384:Hemagglutination; D006358:Hot Temperature; D006801:Humans; D008297:Male; D014521:Urethra / Q000166:cytology / Q000382:microbiology*