Influence of phenothiazines or reserpine on the formation of 14C-glycine from U-14C-serine.
The influence of the psychopharmaceuticals reserpine, thioproperazine and chlorpromazine on the synthesis of 14C-glycine from U-14C-serine was examined in a rat brain preparation. At low serine concentration (2 times 10(-5) mol/l) glycine synthesis in inhibited by all drugs tested probably due to the inhibition of the serine influx into mitochondria. At higher concentration (10(-3) mol/l) and physiological cofactor levels, phenothiazines increase to the measurable amount of 14C-glycine while reserpine has no effect. We assume that at this higher concentration serine transport through the mitochondrial membranes is no longer rate limiting; following phenothiazine action, the membrane-bound glycine cleavage system might be inhibited more strongly than the partly soluble serine hydroxymethyltransferase.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000096:biosynthesis*; D012696:Glycine Hydroxymethyltransferase / Q000378:metabolism; D008297:Male; D010640:Phenothiazines / Q000494:pharmacology*; D051381:Rats; D012110:Reserpine / Q000494:pharmacology*; D012694:Serine / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D010640:Phenothiazines; D012694:Serine; D012110:Reserpine; D012696:Glycine Hydroxymethyltransferase; D005998:Glycine; D002746:Chlorpromazine