[A method for morphometry of bone formation using fluorochromes (author's transl)].
A reliable morphometric determination of newly formed bone requires a continuous labeling of the tissue of interest. A technique is described which uses the fluorochromes Calcein and Xylenolo-range as bone labels. Image contrast in the fluorescence microscope can be increased by means of special filter combinations to permit data collection by computer-compatible video-systems. This technique offers a wide field of applications in experimental bone biology.
D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones / Q000033:anatomy & histology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005998:Glycine / Q000031:analogs & derivatives*; D008856:Microscopy, Fluorescence; D010636:Phenols; D011817:Rabbits; D051381:Rats; D013454:Sulfoxides; D014992:Xylenes
D005452:Fluoresceins; D005456:Fluorescent Dyes; D010636:Phenols; D013454:Sulfoxides; D014992:Xylenes; C016833:xylenol orange; D005998:Glycine; C007740:fluorexon
Methode zur Fluoreszenz--Morphometrie des Knochenanbaus.