Binding of SV40 tumour antigen and Epstein-Barr-virus nuclear antigen to isolated acid-fixed nuclei.
SV40 tumour antigen (TA) bound to methanol-acetic-acid-fixed interphase nuclei and metaphase chromosomes. This reaction was visualized by the anti-complement immunofluorescence test. The reaction was negative when the nuclei had been treated with deoxyribonuclease prior to the addition of antigen, or when TA-antibody negative serum had been used. In parallel tests TA and Epstein-Barr virus nuclear antigen were examined. Both the binding patterns and the optimum conditions for the reaction were similar.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000187:drug effects; D002467:Cell Nucleus / Q000276:immunology*; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral