title: Protein ADP-ribosylation in rat liver cytosol.
abstract: ADP and poly ADP-ribosylation are post-translational modifications of proteins which have been reported to occur essentially in eucaryotic nuclei. This phenomenon has been shown to interfere with a great variety of biological functions (cell differentiation, DNA repair, malignant transformation...). In this paper, we demonstrate for the first time that ADP-ribosylation occurs also in cytosol (120 000 g supernatant) and that several cytosolic proteins can be ADP-ribosylated in rat liver.
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000302:isolation & purification; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
chemical_list: D009702:Nucleoside Diphosphate Sugars; D011506:Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D011064:Poly Adenosine Diphosphate Ribose; D010727:Phosphoric Diester Hydrolases; D014357:Trypsin