Short-term, high-efficiency expression of transfected DNA.
We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.
D000818:Animals; D005796:Genes; D005814:Genes, Viral; D007700:Kinetics; D007739:L Cells / Q000201:enzymology; D051379:Mice; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D013937:Thymidine Kinase