title: Synaptosomes prepared from fresh human cerebral cortex; morphology, respiration and release of transmitter amino acids.
abstract: Synaptosomes prepared from fresh human cerebral cortex were shown to be morphologically similar to those from other species. On incubation, they took up oxygen at a high and linear rate and accumulated potassium against a concentration gradient. In response to depolarization by raised extracellular K+ or addition of veratrine, they showed increased respiration, lowered tissue potassium, and enhanced release of glutamate, aspartate and GABA. The preparation may be of value for studies of neurological disorders.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010101:Oxygen Consumption; D011188:Potassium / Q000032:analysis; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000596:Amino Acids; D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid; D011188:Potassium