Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus.
Two cosmid cloning vectors containing lambda cos sequences and a 42-base-pair multipurpose cloning sequence were constructed. pAD22 also contains a 1.4-kilobase TRP-ARS fragment from Saccharomyces cerevisiae. These cosmids transformed Escherichia coli and S. cerevisiae cells and could be mobilized into Vibrio parahaemolyticus strains with a conjugative plasmid, pRK2013. The cosmid pAD22 was genetically and structurally stable during passage through V. parahaemolyticus and E. coli strains.
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012623:Seawater; D014733:Vibrio / Q000235:genetics*
D004262:DNA Restriction Enzymes