Study of antiosteoporotic agents in tissue culture.
Cultures of osteoblast-like cells were established from calvariae of Sprague-Dawley rats. Pentoxifylline increased cAMP levels and calcium uptake in these cultures. However, calcium uptake increased at lower levels than required to increase cAMP levels. Thus, it is likely that cAMP unrelated mechanisms are also involved in these phenomena.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D010006:Osteoblasts / Q000378:metabolism; D010024:Osteoporosis / Q000188:drug therapy*; D010431:Pentoxifylline / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains; D013805:Theobromine / Q000031:analogs & derivatives*
D000242:Cyclic AMP; D013805:Theobromine; D010431:Pentoxifylline; D002118:Calcium