[Preparation of affinity sorbents for the isolation of phospholipase A2].
The preparation of biospecific organo-silica supports with different concentrations of covalently attached sn-glycero-3-phosphocholine was described. Sorbtion of phospholipase A2 on the hydrophobic analogues of biospecific supports was studied with the aim of elucidating the role of the enzyme-matrix hydrophobic interactions. A comparative characterization of the prepared sorbents was carried out.
D000818:Animals; D002846:Chromatography, Affinity; D003435:Crotalid Venoms / Q000032:analysis; D004546:Elapid Venoms / Q000032:analysis; D008024:Ligands; D010179:Pancreas / Q000201:enzymology; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification*; D054467:Phospholipases A2; D012822:Silicon Dioxide; D013552:Swine
D003435:Crotalid Venoms; D004546:Elapid Venoms; D008024:Ligands; D012822:Silicon Dioxide; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2
Poluchenie affinnykh sorbentov dlia vydeleniia fosfolipazy A2.