Effects of nonspecific smooth muscle relaxants and Ca-blocker on Ca-release and Ca-binding in microsomal fractions from rabbit taenia coli.
Effects of nonspecific smooth muscle relaxants, papaverine (10(-4) M) and Aspaminol (1,1-diphenyl-3-piperidinobutanol hydrochloride, 10(-4) M), and Ca-blocker, D-600 (10(-6) M) on Ca-release and Ca-binding in the microsomal fractions of rabbit taenia coli were determined. The doses necessary to relax the K-depolarized taenia coli were determined. The doses necessary to relax the K-depolarized taenia coli maximally were used. Ca-binding in the microsomal fractions was inhibited by Aspaminol and D-600 and increased by papaverine in the concentration used. On the other hand, Ca-release from the Ca-incorporated microsomal fractions was inhibited by papaverine and Aspaminol but was not influenced by D-600. Changes observed in Ca-transfer may be related to relaxation of smooth muscle induced by the test drugs.
D000818:Animals; D002118:Calcium / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003106:Colon / Q000166:cytology; D005711:Gallopamil / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D010208:Papaverine / Q000494:pharmacology; D010276:Parasympatholytics / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology; D011817:Rabbits; D014700:Verapamil / Q000031:analogs & derivatives*
D010276:Parasympatholytics; D010880:Piperidines; D005711:Gallopamil; D014700:Verapamil; D010208:Papaverine; D002118:Calcium