A simple assay of solubilized adenosine receptors with nitrocellulose membrane filters.
A rapid and simple assay of solubilized adenosine receptors with nitrocellulose membrane filters is described. This assay was sensitive and reproducible when applied to adenosine receptors solubilized from rat brainstem membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Appropriate values of dissociation constants for the solubilized adenosine receptors to their tritiated agonists were obtained by the membrane filter technique. This method should be applicable for the assay of a variety of solubilized receptors.
D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D003101:Collodion; D003902:Detergents; D007700:Kinetics; D008567:Membranes, Artificial; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*; D011983:Receptors, Purinergic; D012995:Solubility
D003902:Detergents; D008567:Membranes, Artificial; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D019830:Adenosine-5'-(N-ethylcarboxamide); D003101:Collodion; D000241:Adenosine