Co-localization of SV40 T antigen and p53 with immunological analogues of microtubule-associated protein-1 on the nuclear skeleton.
Rat SV-3Y1 cells were stained with double immunofluorescence. Treatment of cells with detergent and salt removed about 80% of the antigens and revealed immunofluorescent flecks of the SV40 large T antigen and p53 bound to the nuclear skeleton. These flecks exactly corresponded to the fluorescent spots produced by antibodies against microtubule associated protein-1. The MAP-1 analogues may function in the initiation of DNA synthesis through the interaction with T-antigen and p53.
D000818:Animals; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D051381:Rats; D013539:Simian virus 40; D014764:Viral Proteins / Q000378:metabolism
D000957:Antigens, Viral, Tumor; D008869:Microtubule-Associated Proteins; D014764:Viral Proteins