title: The identification of cyclic nucleotides from living systems using collision-induced dissociation of ions generated by fast atom bombardment mass spectrometry.
abstract: Extracts derived from rat liver and Phaseolus leaves are shown, by collision-induced dissociation of [MH]+ ions generated by fast atom bombardment mass spectrometry, to contain cytidine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate respectively, and not the 2',3'-cyclic isomers. Interference peaks, expected to be common to all mass-analysed ion kinetic energy spectra of ions generated by the fast atom bombardment process from glycerol-based matrices are identified. It is shown that unequivocal identification of cytidine 3',5'-cyclic monophosphate can be made at the microgram level. Attempts to derive a quantitative procedure based on using different cyclic nucleotides as internal standards were unsuccessful due to the poor solubility of these compounds in the matrix system.
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000032:analysis; D003563:Cyclic CMP / Q000032:analysis*; D006152:Cyclic GMP / Q000032:analysis*; D003597:Cytosine Nucleotides / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D010944:Plants / Q000032:analysis*; D051381:Rats
chemical_list: D003597:Cytosine Nucleotides; D003563:Cyclic CMP; D000242:Cyclic AMP; D006152:Cyclic GMP