title: Neuronal, glial and meningeal localizations of neurotransmitter-sensitive adenylate cyclases in cerebral cortex of mice.
abstract: The neurotransmitter-sensitive adenylate cyclases, respectively present in the dissociated cells of new-born mouse cerebral cortex (containing both neuronal and glial cells) and in a homogeneous population of glial cells, were compared. The dissociated cells from the cerebral cortex of new-born mice were found to contain Ca2+-, dopamine-, serotonin- and purinergic-sensitive adenylate cyclases. The dopaminergic receptor involved was extensively characterized and was similar to that described in adult animals. Beta-adrenergic-sensitive adenylate cyclase was present but was poorly active. After 3 weeks in culture, the neurons disappeared and a homogeneous population of glial cells was obtained (96% of the cells synthetized glial fibrillary acidic protein). These glial cells contained a highly potent beta-adrenergic-sensitive adenylate cyclase, and adenosine- adn Ca2+-sensitive enzymes. Ca2+ stimulation of the adenylate cyclase was due to the presence of calmodulin. We suggested that the dopaminergic- and serotoninergic-sensitive adenylate cyclases which disappeared during culture are probably localized in neuronal cells. The presence of Ca2+-, adenosine- and beta-adrenergic-sensitive adenylate cyclases in glial cells does not exclude their presence in neuronal cells. For comparison, the same experiments were conducted on meningeal layers of new-born mice and on meningeal cells in culture. They both contained beta-adrenergic- and purinergic-sensitive adenylate cyclases.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000526:Alprenolol / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000201:enzymology*; D004298:Dopamine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005476:Fluphenazine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007700:Kinetics; D008578:Meninges / Q000201:enzymology*; D051379:Mice; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010646:Phentolamine / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D018377:Neurotransmitter Agents; D012701:Serotonin; D006632:Histamine; D000526:Alprenolol; D000262:Adenylyl Cyclases; D005476:Fluphenazine; D002118:Calcium; D004298:Dopamine; D004837:Epinephrine; D010646:Phentolamine