Restriction enzyme banding of mouse metaphase chromosomes.
Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Alu I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002871:Chromosome Banding; D002875:Chromosomes / Q000502:physiology*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004622:Embryo, Mammalian; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D008815:Mice, Inbred Strains
D004262:DNA Restriction Enzymes