L-glutamate as possible neurotransmitter in the ampullae of Lorenzini of the skate.
Effects of Mg2+, L-glutamate (L-Glu) and L-glutamic acid diethylester (GDEE) on resting and evoked activities of the ampullae of Lorenzini were studied in marine skates (Raja clavata). Perfusion of the basal membrane with 10(-4)-10(-5) M L-Glu produced significant and reversible changes in resting firing rate depending on initial resting rate. When synaptic transmission was blocked by perfusion with elevated Mg2+, the resting activity was restored if L-Glu (10(-4)-10(-5) M) was added. GDEE (10(-3)-10(-4) M) blocked the synaptic transmission. The results are consistent with the view that L-Glu is the synaptic transmitter in the ampullae of Lorenzini of the skate.
D000818:Animals; D005399:Fishes / Q000502:physiology*; D005971:Glutamates / Q000494:pharmacology / Q000502:physiology*; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D018377:Neurotransmitter Agents; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects
D005971:Glutamates; D018377:Neurotransmitter Agents; C011829:glutamic acid diethyl ester; D008274:Magnesium