title: Ontogenesis of muscimol binding sites in cat visual cortex.
abstract: In vitro receptor binding techniques were used to study the characteristics, distribution, and ontogenesis of muscimol binding sites in cat visual cortex. [3H] Muscimol, a GABA agonist, labelled a single population of binding sites with a KD of 18 nM in adult cats. Specific binding was saturable, reversible and was blocked by the addition of GABA or (+) bicuculline. Autoradiograms revealed that the highest density of [3H] muscimol binding occurred in cortical layer IV. Similar patterns of [3H] muscimol binding were observed at all ages examined, although the binding densities differed. The peak [3H] muscimol binding density, corrected for amount of protein, occurred at 3 months postnatally. In 3 day old and adult cats binding density was 41% and 69%, respectively, of the peak value.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002415:Cats; D007700:Kinetics; D009118:Muscimol / Q000378:metabolism*; D009433:Neural Inhibition; D010080:Oxazoles / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000502:physiology; D014793:Visual Cortex / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D010080:Oxazoles; D011963:Receptors, GABA-A; D009118:Muscimol; D005680:gamma-Aminobutyric Acid