The interaction of copper chloride with erythrocyte membrane as a source of activated oxygen species. A chemiluminescent study.
The possibility for the generation of activated oxygen species during the interaction between copper chloride and erythrocyte membranes was investigated. A chemiluminescent method for detecting superoxide radicals and hydrogen peroxide was used. It was found that the interaction of CuCl2 with erythrocyte membrane is accompanied with O-2 and H2O2 generation. On the base of this result it is proposed that the activated oxygen species generated by CuCl2-membrane interaction may be able to initiate peroxidative breakdown processes in erythrocytes eventually leading to haemolysis.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D003300:Copper / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D008165:Luminol; D003300:Copper; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; C029892:cupric chloride