title: pH-dependent adhesion of piliated Corynebacterium renale to bovine bladder epithelial cells.
abstract: The adhesion of Corynebacterium renale 115 to bovine bladder epithelial cells was studied with two clones of the bacteria, piliated (P+) and nonpiliated (P-). The percentage of the cells with attached P+ bacteria was greater than that of the cells with attached P- bacteria, and the number of attached P+ bacteria was higher than that of the P- bacteria. The adhesion of P+ bacteria was found to be pH dependent, which was supported by the following facts: the number of attached bacteria was high at a pH above 7.6 and low at a pH below 6.8; the adhering P+ bacteria were eluted immediately after the pH was decreased from 7.4 to 6.4; and the P+ bacteria unattached at pH 6.4 adhered to the cells immediately after the pH was increased to pH 7.4.  The adhesion of P+ bacteria was inhibited by anti-pilus antiserum. The adhesion was not inhibited by amino acids or sugars, including mannose, and was not influenced by Ca2+ and Mg2+. P- bacteria hardly attached to the epithelial cells, irrespective of the pH and other factors.
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002417:Cattle; D003352:Corynebacterium / Q000502:physiology* / Q000648:ultrastructure; D004848:Epithelium / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013696:Temperature; D001743:Urinary Bladder / Q000166:cytology / Q000382:microbiology*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates