title: Degradation of DNA by silicic acid.
abstract: S1 nuclease hydrolysis and hydroxyapatite chromatography were used to study the effect of silicic acid on DNA. Native calf thymus DNA was incubated with increasing concentrations of silicic acid (DNA nucleotide/silicic acid molar ratios of 1:0.25, 1:0.5 and 1:1) and subjected to S1 nuclease hydrolysis. An increasing degree of DNA degradation was seen suggesting a destabilization of the secondary structure. A decrease in melting temperature was also observed. Hydroxyapatite chromatography indicated that incubation at the molar ratio of 1:1 resulted in denaturation and degradation of DNA.
mesh_terms: D000818:Animals; D002417:Cattle; D002845:Chromatography; D004247:DNA; D017886:Durapatite; D004720:Endonucleases; D006868:Hydrolysis; D006882:Hydroxyapatites; D009691:Nucleic Acid Denaturation; D012824:Silicic Acid; D012822:Silicon Dioxide; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013950:Thymus Gland
chemical_list: D006882:Hydroxyapatites; D012824:Silicic Acid; D012822:Silicon Dioxide; D004247:DNA; D017886:Durapatite; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases