[Synthesis of a 33-member polynucleotide containing the "core" att site of phage lambda DNA and its cloning].
Two polynucleotides containing 33 monomeric units were synthesized by a solid-phase phosphotriester method. These polynucleotides form a duplex with protruding 5'-ends, which allows to clone the duplex in EcoRI site of a cloning vehicle. Each polynucleotide was purified by electrophoresis in polyacrylamide gel, and the duplex obtained was cloned in EcoRI site of pUR 222 plasmid DNA. The structure of the cloned duplex containing the "core" att site of phage lambda was confirmed by sequencing.
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D005814:Genes, Viral; D010957:Plasmids; D011119:Polynucleotides / Q000138:chemical synthesis*
D004279:DNA, Viral; D011119:Polynucleotides; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI
Sintez 33-chlennogo polinukleotida, soderzhashchego "core" att saĭt DNK bakteriofaga lambda, i ego klonirovanie.