title: Effects of 12-O-tetradecanoyl phorbol-13-acetate and epidermal growth factor on the proliferation of human mutant fibroblasts in vitro.
abstract: Cultures of skin fibroblasts (SF) from individuals with hereditary adenomatosis of the colon and rectum (ACR) and from normal individuals were exposed to 12-O-tetradecanoyl phorbol-13-acetate (TPA) (0.5-100 ng/ml), and epidermal growth factor (EGF) (0.01-10.0 ng/ml) at both low and high cell densities. Cell proliferation of low density cultures (4.75 x 10(3) cells/cm2) was inhibited by TPA, while cell proliferation of high density cultures (6 x 10(4) cells/cm2) was stimulated by TPA. EGF was mitogenic at both cell densities of normal and ACR-SF cultures. TPA and EGF each produced characteristic changes in the morphology of ACR-SF colonies. These results suggest that EGF and TPA may share only 1 class of receptors, and that TPA may be used to monitor cancer progression.
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D009377:Multiple Endocrine Neoplasia / Q000235:genetics / Q000473:pathology*; D009154:Mutation; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D010704:Phorbols; D004815:Epidermal Growth Factor; D013755:Tetradecanoylphorbol Acetate