Grafting of burns with cultured epithelium prepared from autologous epidermal cells.
The cells from a small piece of epidermis can be grown into a large number of cultured epithelia. Such epithelia, generated from autologous skin, were grafted onto full-thickness burn wounds in two patients. The cultured epithelia acquired an epidermal structure resembling  that achieved with conventional split-thickness skin grafts, and survived for the period of observation (up to 8 months). Since the method of cultivation can generate large amounts of epithelium, the procedure is applicable to the grafting of large areas, as in severe burns.
D000328:Adult; D002056:Burns / Q000601:surgery*; D002058:Burns, Electric / Q000601:surgery; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004815:Epidermal Growth Factor / Q000008:administration & dosage; D004817:Epidermis / Q000637:transplantation*; D004847:Epithelial Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D014182:Transplantation, Autologous
D004815:Epidermal Growth Factor