Different properties in lymphoblastoid cell lines from patients with Bloom syndrome.
In 3 lymphoblastoid cell lines derived from 3 patients with Bloom syndrome (BS), the baseline frequency of sister chromatid exchanges (SCEs), chromosomal breakage, sensitivity to ethylmethane-sulfonate (EMS), and bromodeoxyuridine (BrdUrd) were examined. The SCE frequency of 2 BS lines (EB-BS-NoKi-2 and EB-BS-AkSak) was about 2 to 2.5 times those of the normal cell lines, while that of another BS line (EB-BS-2KA) was about 10 to 11 times. The net increase in the number of SCEs in EB-BS-NoKi-2 and EB-BS-AkSak lines induced by EMS was similar to that of normal cell lines, but it was high in the BS-2KA line. Sensitivity to BrdUrd was examined using SCE induction at different concentrations of BrdUrd and by cell cycle analysis. EB-BS-NoKi-2 and EB-BS-AkSak lines were no more sensitive than normal cell lines, while the EB-BS-2KA line was more sensitive than controls. High frequency of chromosomal breakage was found only in the EB-BS-2KA line. These results suggest that 2 types of cells exist in the B-lymphoblastoid cells of BS.
D001402:B-Lymphocytes / Q000648:ultrastructure*; D001816:Bloom Syndrome / Q000235:genetics*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D012854:Sister Chromatid Exchange / Q000187:drug effects
D005020:Ethyl Methanesulfonate; D001973:Bromodeoxyuridine