Protein separations on reversed-phase high-performance liquid chromatography minicolumns.
The resolution of cytochrome c, bovine serum albumin and ovalbumin on reversed-phase columns under gradient elution conditions was found to be constant or to improve as column length was decreased from 45 to 6.3 mm. The resolution remained constant even when the column length decreased to 1.6 mm. Recovery of protein in the first gradient cycle was improved by the use of short columns.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003574:Cytochrome c Group / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D010047:Ovalbumin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
D003574:Cytochrome c Group; D007202:Indicators and Reagents; D011506:Proteins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin