The release of (3H)-GABA from the rabbit retina.
The neuronal and glial release of (3H)-GABA from rabbit retina has been studied. The results indicate, that neither are there any glutamate, aspartate or glycine receptors on the GABA accumulating neurons, nor any GABA autoreceptors. (3H)-GABA was found to be released by 40 mM K+ from retinal neurons, but not from glia, and the release was not dependent on extracellular Ca++. This indicates a release from a non vesicular transmitter pool. Ouabain has been proposed as a pharmacological tool for studying the release of (3H)-GABA located in neuronal cytoplasm. However, it induced an increased release of (3H)-GABA from both neurons and glia and it is therefore unlikely that it can be used for the specific purpose of studying release from neuronal cytoplasm.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D005260:Female; D005998:Glycine / Q000494:pharmacology; D008297:Male; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D017459:Receptors, Amino Acid; D011963:Receptors, GABA-A / Q000187:drug effects; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects; D012160:Retina / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000596:Amino Acids; D017459:Receptors, Amino Acid; D011963:Receptors, GABA-A; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; C031959:aspartic acid receptor; D005680:gamma-Aminobutyric Acid; D010042:Ouabain; D011188:Potassium; D005998:Glycine