Specific receptor for the opioid peptide dynorphin: structure--activity relationships.
The structural features responsible for the high potency and opiate receptor specificity of the opioid peptide dynorphin in the guinea pig ileum myenteric plexus were examined. Successive removal of COOH-terminal amino acids from dynorphin-(1--13) demonstrated important contributions of lysine-13, lysine-11, and arginine-7 to the potency. Removal of the NH2-terminal tyrosine abolished the biologic activity. Several other structural modifications were shown to affect potency: substitution of D-alanine for glycine-2 reduced the potencies of dynorphin-(1--13) amide, -(1--11), and -(1--10); and methyl esterification of the COOH terminus enhanced the potencies of dynorphin-(1--12), -(1--10), -(1--9), -(1--8), and -(1--7). Within the dynorphin sequence, lysine-11 and arginine-7 were found to be important for selectivity of interaction with the dynorphin receptor, which is distinguishable from the mu receptor in this tissue.
D000818:Animals; D001681:Biological Assay; D004399:Dynorphins; D004723:Endorphins / Q000378:metabolism* / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D009197:Myenteric Plexus / Q000187:drug effects; D011957:Receptors, Opioid / Q000378:metabolism*; D013329:Structure-Activity Relationship
D004723:Endorphins; D011957:Receptors, Opioid; C030738:dynorphin receptor; D004399:Dynorphins