The effect of prostaglandins I2, E1, E2 and dibutyryl cyclic AMP on the cytoplasmic spreading of rat osteoclasts.
We isolated osteoclasts from neonatal rat bone and assessed the effects of prostaglandins (PGs) I2, E1 and E2 on osteoclastic spreading. We found that although the PGs and dibutyryl cyclic AMP (DB-cAMP) acted as direct inhibitors of osteoclastic spreading, if osteoblasts and osteoclasts were co-cultured, the addition of PGs or DB-cAMP caused a considerable increase in spreading. This suggests that the PGs and DB-cAMP induce osteoblasts to stimulate osteoclasts. Osteoblasts are known to produce PGs, and thus possess the capacity to either inhibit (through PGs) or stimulate osteoclasts. Our results suggest that the balance between stimulation and inhibition of osteoclasts by osteoblasts may be determined by osteoblastic cyclic AMP levels.
D000527:Alprostadil; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D003595:Cytoplasmic Streaming / Q000187:drug effects*; D015232:Dinoprostone; D011464:Epoprostenol / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D011453:Prostaglandins; D011458:Prostaglandins E; D011464:Epoprostenol; D000242:Cyclic AMP; D000527:Alprostadil; D015232:Dinoprostone