Solubilization of neuroleptic/dopamine receptors of human brain striatum.
Dopaminergic neuroleptic receptors were solubilized by digitonin from post-mortem human brain putamen. The receptors were labelled by [3H]spiperone and separated from free [3H]spiperone by sephadex chromatography. Neuroleptics and neurotransmitters had similar inhibitory and stereoselective potencies on the solubilized and intact membrane receptors. The KD for [3H]spiperone was apparently high for the solubilized receptors. Unlike the rat striatum, therefore, the human putamen can be solubilized to release neuroleptic/dopamine receptors, permitting the future development of highly sensitive methods for detecting dopamine receptors.
D014150:Antipsychotic Agents / Q000378:metabolism* / Q000494:pharmacology; D003342:Corpus Striatum / Q000378:metabolism*; D004072:Digitonin; D006801:Humans; D018377:Neurotransmitter Agents / Q000494:pharmacology; D011954:Receptors, Dopamine / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D013134:Spiperone / Q000378:metabolism
D014150:Antipsychotic Agents; D018377:Neurotransmitter Agents; D011954:Receptors, Dopamine; D013134:Spiperone; D004072:Digitonin