Analysis of the Sendai virus M gene and protein.
The nucleotide sequence of the Sendai virus M (matrix or membrane) gene region was determined from cloned genomic DNA, and the limits of the M mRNA were determined by S1 nuclease mapping. The M mRNA is 1,173 nucleotides long and contains a single long open reading frame coding for a protein of 348 amino acids. The amino acid sequences of the N- and C-terminal peptides of the M protein were obtained by mass spectrometric analysis and correspond to those predicted from the open reading frame, with the N terminus modified in vivo by cleavage of the initiating methionine and acetylation of the following amino acid. The amphiphilic nature of the M protein structure is discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D013058:Mass Spectrometry; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*
D000596:Amino Acids; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D004262:DNA Restriction Enzymes