Correlation of the effect of beta-lactamase inhibitors on the beta-lactamase in growing cultures of gram-negative bacteria with their effect on the isolated beta-lactamase.
The effectiveness of clavulanic acid, sulbactam, quinacillin sulfone, and the carbapenems MM13902 and MM4550 as inhibitors of TEM-2 beta-lactamase in growing cultures of gram-negative bacteria has been studied. Each of these beta-lactams inhibited the enzyme in intact cells, and the nature of the inhibition correlated with studies on the purified enzyme. The potency of these compounds as inhibitors of the beta-lactamase in vivo can be correlated with the amounts hydrolyzed by the purified enzyme under saturating conditions during the inhibition of the enzyme in vitro.
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology*; D002969:Clavulanic Acids / Q000494:pharmacology*; D007769:Lactams; D010397:Penicillanic Acid / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D013407:Sulbactam; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D007769:Lactams; D010406:Penicillins; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; C020786:olivanic acid; C033810:quinacillin sulfone; D000667:Ampicillin; D010397:Penicillanic Acid; D013407:Sulbactam