title: In vitro and in vivo studies with an avian reovirus derived from a temperature-sensitive mutant clone.
abstract: A temperature-sensitive (ts) mutant of avian reovirus isolate Maine 1-203 was obtained at 41 C. The 19th passage of this mutant (ts 19) was utilized for in vitro studies comparing its replication in macrophage cultures with that of its parent strain and an apathogenic S1133 virus. In vivo studies were initiated to determine the ts mutant's pathogenicity for day-old chicks, safety and protective levels for 6-day-old chicks, and interference with Marek's disease, Newcastle, and infectious bronchitis vaccinations. Although the results of in vitro trials indicated no difference among the three reoviruses in the in vitro pathogenicity for macrophage cultures, in vivo studies found the mutant to differ from its parent strain in pathogenicity for day-old chicks. When the ts mutant was administered at 6 days of age, it was a safe and effective vaccine against viral arthritis, with no resulting suppression of other vaccine responses.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D006564:Herpesviridae / Q000276:immunology; D001351:Infectious bronchitis virus / Q000276:immunology; D008264:Macrophages / Q000382:microbiology; D009154:Mutation; D009522:Newcastle disease virus / Q000276:immunology; D012087:Reoviridae / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D013696:Temperature; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
chemical_list: D014765:Viral Vaccines