Evidence for a role of the cytoskeleton in the in vitro folliculogenesis of the thyroid gland of the fetal rat.
Thyrotropic hormone (TSH) or cAMP accelerate the formation of follicular cavities in the explanted thyroid gland of the 15-day-old rat fetus. Cytochalasin B or vinblastine and nocodazole or colchicine, which disorganize microfilamental and microtubular structures respectively, inhibit or completely block in vitro-induced folliculogenesis. Exposure of the thyroid tissue to lumicolchicine, a structural isomer of colchicine deprived of antimicrotubular activity, does not inhibit the activation of folliculogenesis induced by TSH. These results are strong evidence for the supposition that microfilaments and microtubules are involved in the TSH-stimulated mechanisms resulting in thyroid folliculogenesis. Folliculogenesis requires the integrity of both microfilaments and microtubules.
D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000648:ultrastructure*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D005333:Fetus; D008162:Lumicolchicines / Q000494:pharmacology; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure*; D013972:Thyrotropin / Q000494:pharmacology
D008162:Lumicolchicines; D003571:Cytochalasin B; C042109:lumicolchicine; D013972:Thyrotropin; D000242:Cyclic AMP; D004121:Dimethyl Sulfoxide