Rate of decrease of glutamyltransferase and acid phosphatase activities in the human vagina after coitus.
I studied the rate at which gamma-glutamyltransferase (EC 2.3.2.2) and acid phosphatase (EC 3.1.3.2) activities decrease in the healthy human vagina after coitus. Specimens from the coital and non-coital vagina, and from the rectum and buccal cavity, and pre-ejaculate lubricating fluid were also assayed for both enzymes. Prostatic acid phosphatase was determined with use of two substrates: thymolphthalein monophosphate and alpha-naphthyl phosphate (tartrate-inhibitable fraction). Glutamyltransferase was assayed with gamma-glutamyl-p-nitroanilide as substrate. All non-coital specimens were negative for glutamyltransferase activity; the activity of acid phosphatase varied with type of substrate and type of specimen. The equation y = a + (b/x) describes the decline in activity (y) of either acid phosphatase or glutamyltransferase in the vagina of healthy women post-coitally with time (x).
D000135:Acid Phosphatase / Q000032:analysis*; D003075:Coitus; D004542:Ejaculation; D005260:Female; D006801:Humans; D008297:Male; D008433:Mathematics; D008598:Menstruation; D000439:Pentanols / Q000494:pharmacology; D012661:Semen / Q000201:enzymology; D013997:Time Factors; D014621:Vagina / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D000439:Pentanols; D005723:gamma-Glutamyltransferase; D000135:Acid Phosphatase