title: A comparison of IHA and ELISA tests in the detection of antibodies to entamoeba histolytica.
abstract: In this report we have evaluated the sensitivity and specificity of IHA and ELISA for the detection of anti-amebic antibodies in the serum samples of 147 patients with poor mental and hygienic status hospitalized in mental institutions. Our data have demonstrated a positive agreement between ELISA and IHA on positive sera. The major drawback of IHA is the occurrence of false negative results, 13/82 sera negative in IHA (less than 1/128) are positive in the ELISA. Furthermore, the ELISA is useful for serological diagnosis of amebiasis in the laboratories of low endemic areas.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D000906:Antibodies