Separation of two populations of cells with gamma-glutamyl transpeptidase from carcinogen-treated rat liver.
Noninbred Sprague-Dawley rats were maintained on a choline-deficient diet containing 0.05% ethionine. After 10-13 weeks, livers were dispersed with collagenase, lysozyme, collagenase and hyaluronidase. Pronase, or a selected batch of trypsin. The highest yield of cells with histochemically demonstrable gamma-glutamyl transpeptidase (GGT) was obtained with trypsin. After velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium, two modal populations of cells with histochemically demonstrable GGT were observed. The first mode contained cells that were morphologically different from hepatocytes and that may be oval cells. The second, more rapidly sedimenting modal population of cells with GGT was morphologically similar to hepatocytes as assessed with Wright's stain; the location of this population in the gradient was the same as the location of cells with the appearance of hepatocytes that lacked iron and that had decreased glucose 6-phosphatase. In multiple experiments, the purest fractions contained 71.7 +/- 3.5% cells (mean +/- SD) with the appearance of hepatocytes with histochemically demonstrable GGT.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D004796:Clinical Enzyme Tests; D005001:Ethionine / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006651:Histocytochemistry; D007501:Iron / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000175:diagnosis; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000175:diagnosis; D011230:Precancerous Conditions / Q000175:diagnosis*; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D007501:Iron; D005723:gamma-Glutamyltransferase; D005952:Glucose-6-Phosphatase; D005001:Ethionine