The effect of cholera toxin on choroid plexus carbonic anhydrase activity in vitro.
The effects of the adenylate cyclase agonists cholera toxin and prostaglandin E2 on carbonic anhydrase activity in vitro was measured in choroid plexuses isolated from Sprague-Dawley rats. Choroid plexuses were incubated in buffer at 38 degrees C (pH 7.4) with either cholera toxin or prostaglandin E2 (PGE2) at a concentration and for a time period that had been shown in earlier studies to result in maximal stimulation of cyclic AMP production. Cholera toxin (10 micrograms/ml) caused a twofold increase (p < .001) in choroid plexus carbonic anhydrase activity when cholera toxin treated plexuses [20.92 +/- .46 mol CO2/(min)(mg protein X 10(-8)] were compared with plexuses exposed to heat inactivated cholera toxin (10.92 +/- .43). When choroid plexuses were homogenized and separated into a 10,000 g pellet and a supernatant fraction, the supernatant carbonic anhydrase was unresponsive to cholera toxin stimulation. In the pellet fraction, which contained all the cellular adenylate cyclase, challenge with cholera toxin produced a significant increase in carbonic anhydrase activity (p < .01). Control activity was 10.9 +/- 1.2 mol CO2/(min)(mg protein X 10(-8), while carbonic anhydrase activity in fractions exposed to cholera toxin was 28.4 +/- 0.8. PGE2 had no effect, however, upon choroid plexus carbonic anhydrase activity. Since both PGE2 and cholera toxin stimulate cyclic AMP production in vitro, a compartmental model of secretory control is proposed.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002772:Cholera Toxin / Q000494:pharmacology*; D002831:Choroid Plexus / Q000201:enzymology*; D000242:Cyclic AMP / Q000096:biosynthesis; D008297:Male; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats
D011458:Prostaglandins E; D003994:Bucladesine; D002772:Cholera Toxin; D000242:Cyclic AMP; D002256:Carbonic Anhydrases