A quantitative fluorimetric assay for the determination of oxidant production by polymorphonuclear leukocytes: its use in the simultaneous fluorimetric assay of cellular activation processes.
A fluorimetric assay for the indirect determination of superoxide production during the respiratory burst of stimulated polymorphonuclear leukocytes was described. The method allowed the determination of submicromolar concentrations of superoxide, and was sufficiently sensitive that first-derivative kinetic analysis of the respiratory burst could be quickly analyzed. Conditions for the simultaneous fluorimetric analysis of superoxide production and intracellular calcium fluxes were described.
D000634:Aminoquinolines; D002118:Calcium / Q000097:blood; D003574:Cytochrome c Group / Q000378:metabolism; D005456:Fluorescent Dyes; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence; D013481:Superoxides / Q000096:biosynthesis / Q000097:blood*
D000634:Aminoquinolines; D003574:Cytochrome c Group; D005456:Fluorescent Dyes; D005609:Free Radicals; D013481:Superoxides; C035739:Quin2; D002118:Calcium