Non-immunological precipitation by the neutral detergent triton X-100 in agar gel diffusion.
Triton X-100 can be used to clarify vague immunoprecipitin lines from bacterial antigens; however, non-immunological precipitation can lead to mistaken interpretation of immunodiffusion results. If Triton X-100 is added directly to the gel during preparation rather than to the antigen well, this detergent artifact can be eliminated.
D000942:Antigens, Bacterial / Q000032:analysis*; D001439:Bacteroides / Q000276:immunology; D005779:Immunodiffusion / Q000379:methods*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D001443:Prevotella melaninogenica / Q000276:immunology
D000942:Antigens, Bacterial; D011092:Polyethylene Glycols