title: The H+/O ratio of proton translocation linked to the oxidation of succinate by mitochondria.
abstract: In a recent communication Lehninger and co-workers (Costa, L.E., Reynaferje, B., and Lehninger, A.L. (1984) J. Biol. Chem. 259, 4802-4811) reported values approaching 8 for the H+/O ratio of vectorial proton ejection from rat liver mitochondria respiring with succinate. Here we present a rigorous analysis of these measurements which reveals that they may significantly overestimate the true H+/O stoicheiometry.
mesh_terms: D000818:Animals; D004566:Electrodes; D005189:False Positive Reactions; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011522:Protons; D051381:Rats; D012044:Regression Analysis; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid
chemical_list: D011522:Protons; D013386:Succinates; D019802:Succinic Acid