Presence of dynorphin-like immunoreactivity in rat pituitary gland and hypothalamus.
Using a highly specific and sensitive radioimmunoassay for dynorphin(1-13), dynorphin-like immunoreactivity (dynorphin-LI) was detected in rat pituitary and hypothalamus. Gel chromatographic studies on Sephadex G-50 revealed three components of dynorphin-LI with molecular weights of approximately 7500-9500 (big dynorphin), 3500-5500 (intermediate dynorphin) and 1600-1900 (small dynorphin), the latter of which eluted at the same position as authentic dynorphin contamination in porcine ACTH extracts (Sigma). Dynorphin-LI in rat anterior pituitary existed mainly as big dynorphin, whereas dynorphin-LI in rat intermediate-posterior pituitary and hypothalamus eluted mainly at the position of authentic small dynorphin.
D000818:Animals; D002850:Chromatography, Gel; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis*; D007031:Hypothalamus / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats
D004723:Endorphins; D010446:Peptide Fragments; C026843:dynorphin (1-13); D004399:Dynorphins