title: Volume flow, hydraulic conductivity and electrical properties across bovine tracheal epithelium in vitro: effect of histamine.
abstract: Volume flow (Jv), potential difference (delta psi), short-circuit current (io) and electrical resistance (R) were measured simultaneously across bovine tracheal epithelium in vitro. Under basal conditions, with no applied hydrostatic or osmotic pressure gradients (delta P = 0, delta phi = 0), no spontaneous Jv was observed. delta psi was 31 +/- 2 mV (lumen negative ), io 161 +/- 8 microA cm-2 and R 202 +/- 9 omega cm2, n = 50. When a delta pi was applied, by adding 20 - 80 mM sucrose into the medium bathing either the luminal or the serosal side of the tissue, a linear relationship was found between delta pi and Jv toward the lumen or toward the serosa. The apparent hydraulic conductivity (apparent Lp) was 4.6 - 4.9 10(-6) cms-1 atm-1. Histamine 10(-4) M did not induce any spontaneous Jv under basal conditions and had no effect on io nor on R. However, histamine caused a 100% increase in Jv elicited by sucrose gradients. It was concluded that histamine exerts a selective action on the hydraulic conductivity of bovine tracheal epithelium. Experiments using H1-receptors antagonists (diphenhydramine, dimetindene, chloropyramine) and H2-antagonists (cimetidine, metiamide) or a H2-agonist (impromidine) showed that the increase of Lp induced by histamine was mediated via H2-receptors.
mesh_terms: D000818:Animals; D002417:Cattle; D004553:Electric Conductivity; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D014132:Trachea / Q000187:drug effects / Q000502:physiology*
chemical_list: D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D006632:Histamine