[Splitting activity of gastrointestinal mucosa using paba-peptide as a substrate (author's transl)].
Splitting activity of gastrointestinal mucosa was investigated by using N-benzoyl-L-tyrosyl-p-aminobenzoicacid (PABA-peptide) in 24 surgical materials obtained from all parts of the human gastrointestinal section. The highest activity was found in the adult jejunum (2.92-4.72 mIE/cm2 intact mucosa). The splitting activity of homogenates, prepared from correspondingly sized mucosa, always was 3-5 times higher than that of the intact mucosa; it was totally inhibited by NCDC, a specific inhibitor for chymotrypsin. The solid linkage and probably also the accumulation of very stable chymotrypsin on the mucosa seem to be responsible for the low sensitivity of the Pabapeptide-test. It is possible that the specificity of this test is considerably influenced by the dependency of the linkage capacity on the morphological structure of the mucosa.
D010129:4-Aminobenzoic Acid / Q000378:metabolism*; D000328:Adult; D062365:Aminobenzoates / Q000378:metabolism*; D002648:Child; D002918:Chymotrypsin; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D011485:Protein Binding; D062366:para-Aminobenzoates
D062365:Aminobenzoates; D062366:para-Aminobenzoates; C006717:bentiromide; D002918:Chymotrypsin; D010129:4-Aminobenzoic Acid
Spaltaktivität menschlicher Magen-Darmschleimhaut gegenüber PABA-Peptid.