Localisation of malignant glioma by a radiolabelled human monoclonal antibody.
Human monoclonal antibodies were produced by fusing intratumoral lymphocytes from patients with malignant gliomas with a human myeloma line. One antibody was selected for further study after screening for binding activity to glioma cell lines. The patient from whom it was derived developed recurrent glioma. 1 mg of antibody was purified, radiolabelled with 131I, and administered intravenously. The distribution of antibody was determined in the blood, CSF and tumour cyst fluid and compared with that of a control human monoclonal immunoglobulin. Antibody localisation in the tumour was observed and confirmed by external scintiscanning.
D000328:Adult; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000032:analysis*; D001345:Autoradiography; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D005910:Glioma / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D009364:Neoplasm Recurrence, Local; D016719:Radioimmunodetection
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G