title: Autophagocytosis in mouse seminal vesicle cells in vitro. Temperature dependence and effects of vinblastine and inhibitors of protein synthesis.
abstract: Spontaneous autophagocytosis was observed in mouse seminal vesicle cells during incubation for 2 h in vitro. The number of autophagic vacuoles formed was greatest at 37 degrees C and decreased when the temperature was lowered. At 22 degrees C it reached the near-zero value characteristic of non-incubated control cells. Incubation of the cells at 37 degrees C in the presence of 0.1 mg/ml vinblastine sulfate resulted in a marked increase in the number of autophagic vacuoles, but the drug was ineffective at 22 degrees C. Puromycin (10(-3) M) exerted no influence on spontaneous autophagocytosis, but cycloheximide in concentrations from 10(-7) M to 10(-3) M inhibited both spontaneous and vinblastine-induced autophagocytosis. The formation of tubulin paracrystals in vinblastine treated cells was not prevented either by low (22 degrees C) temperature or in the presence of cycloheximide.
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D008297:Male; D051379:Mice; D010587:Phagocytosis; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012669:Seminal Vesicles / Q000187:drug effects / Q000473:pathology*; D013696:Temperature; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D011691:Puromycin; D014747:Vinblastine; D003513:Cycloheximide