DNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.
Cell lines that have high levels of resistance to beta-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in two steps from Chinese hamster ovary cells. Resistance to beta-aspartylhydroxmate was transferred into sensitive cells by using total genomic DNA derived from the dominant two-step mutants. The surviving colonies were characterized as transferants on the basis of transfer frequency, degree of resistance to beta-aspartylhydroxamate, increased level of asparagine synthetase activity, expression of the donor form of asparagine synthetase, codominance in hybrids, and instability of the phenotype in the absence of selection.
D000818:Animals; D001216:Asparagine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001217:Aspartate-Ammonia Ligase / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D004274:DNA, Recombinant; D004351:Drug Resistance; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D008025:Ligases / Q000235:genetics*; D010053:Ovary; D013696:Temperature; D014170:Transformation, Genetic
D004274:DNA, Recombinant; C024848:beta-aspartylhydroxamic acid; D001216:Asparagine; D008025:Ligases; D001217:Aspartate-Ammonia Ligase