Solubilization and properties of a benzodiazepine receptor from calf cortex.
A putative benzodiazepine receptor was solubilized from calf cortex by the use of sodium deoxycholate as an 10.9S entity containing subunits of which at least part are of 51 K molecular weight. The solubilized receptor retains its high affinity for [3H]flunitrazepam. The affinity for various other drugs, including benzodiazepines and xanthine derivatives, was also not significantly altered. GABAergic modulation of the receptor affinity for [3H]flunitrazepam was diminished but still detectable.
D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism*; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D005445:Flunitrazepam / Q000378:metabolism; D008970:Molecular Weight; D011955:Receptors, Drug / Q000378:metabolism*; D012995:Solubility; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D014151:Anti-Anxiety Agents; D011955:Receptors, Drug; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam