[Calcium and somatostatin interaction in regulating prolactin and somatotropic hormone release from rat adenohypophysis cells in primary culture].
The effect of Ca+2 and somatostatin on prolactin and growth hormone (GH) secretion was studied in the primary cell culture of the rat adenohypophysis. A decrease in Ca+2 concentration of the medium from 1.8 to 0.1 mM inhibited prolactin release and did not influence GH secretion. An elevation of extracellular Ca+2 level up to 5 mM did not affect prolactin secretion and slightly decreased GH release. Somatostatin inhibited prolactin and GH release provided Ca+2 concentration in the medium was normal (1.8 mM) and/or diminished (0.1 mM). An increase in Ca+2 level up to 5 mM blocked the inhibitory effect of somatostatin. The results obtained show Ca+2 and somatostatin to be antagonistic in the prolactin control and GH release from adenohypophyseal cells.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002413:Cations, Divalent; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D013004:Somatostatin / Q000502:physiology*
D002413:Cations, Divalent; D013004:Somatostatin; D011388:Prolactin; D013006:Growth Hormone; D002118:Calcium
Vzaimodeĭstvie kal'tsiia i somatostatina v reguliatsii osvobozhdeniia prolaktina i somatotropnogo gormona iz kletok adenogipofiza krysy v pervichnoĭ kul'ture.