Bovine serum albumin: characterization of a fatty acid binding site on the N-terminal peptic fragment using a new spin-label.
A new spin-label, 4-(L-glutamo)-4'-[(1-oxy-2,2,5,5-tetramethyl-3L-pyrrolidinyl )amino]-3, 3'-dinitrodiphenyl sulfone, is shown to bind to one high-affinity binding site on bovine serum albumin (K = 5 X 10(4) M-1, n = 1). Analysis of the binding of the spin-label to the amino-terminal half (peptic fragment PB) and the carboxy-terminal half (peptic fragment PA) of BSA, and their complex (PA-PB), indicates that the spin-label binds to a long-chain fatty acid binding site located on PB. The usefulness of the novel specificity of the spin-label in characterizing this binding site is discussed.
D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D005227:Fatty Acids / Q000378:metabolism*; D005973:Glutamine / Q000031:analogs & derivatives* / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013113:Spin Labels; D013450:Sulfones / Q000378:metabolism*
D005227:Fatty Acids; D010168:Palmitates; D010446:Peptide Fragments; D013113:Spin Labels; D013450:Sulfones; D005973:Glutamine; D012710:Serum Albumin, Bovine; C044829:4-(glutamo)-4'-((1-oxy-2,2,5,5-tetramethyl-3-pyrrolidinyl)amino)-3,3'-dinitrophenyl sulfone