Monoclonal antibodies and specific cell surface receptors do not discriminate between human growth hormone prepared by DNA recombinant techniques and the native hormone.
Native and E. coli-derived human growth hormone (hGH) have been compared in their ability to react with four different monoclonal antibodies (MAbs) as well as with the specific cell surface receptors of cultured human IM-9 lymphocytes and liver membranes of pregnant rabbit. For all the tests used, the competition curves obtained using both hormone preparations were superimposable. The results suggest a close similarity in the immunological and biological (binding reaction) properties of native and recombinant hGH.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000276:immunology*; D013006:Growth Hormone / Q000276:immunology*; D006801:Humans; D011956:Receptors, Cell Surface / Q000276:immunology
D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D011956:Receptors, Cell Surface; D013006:Growth Hormone