Basal cyclic AMP levels in human blood mononuclear cells.
Basal cyclic AMP levels in blood mononuclear cells from healthy human donors has been studied. A great variation in cyclic AMP content ranging from below 2 to 20 pmol 10(-6) cells was seen. Incubation of the cells at 37 degrees C mostly showed a time-dependent decrease in basal cyclic AMP, levelling off after 16 h. When treated synchronously, cells from blood from the first sample of blood sampling contained more cyclic AMP than that of later samples. Presence of adenosine deaminase, theophylline or indomethacin influenced cyclic AMP concentrations in a non-consistent manner. Variations in cyclic AMP content may in some individuals be ascribed to early increase of cyclic AMP, in others to a progressive reduction in basal cyclic AMP during blood sampling, cell preparation and incubation.
D000241:Adenosine / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000097:blood; D000242:Cyclic AMP / Q000097:blood*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D013806:Theophylline / Q000494:pharmacology
D013806:Theophylline; D000242:Cyclic AMP; D000243:Adenosine Deaminase; D000241:Adenosine; D007213:Indomethacin