Cloning and molecular analysis of the finO region from the antibiotic-resistance plasmid R6-5.
The cloning of the finO region from the antibiotic-resistance plasmid R6-5 is reported. On the basis of DNA deletion analysis and Tn5 transposon insertional mutagenesis of finO+ chimeric plasmids, finO has been located within the coordinates 94.0-94.85 on the R6-5 map. A 32,000-Da polypeptide (32K), which is encoded within 92.75-94.25R6-5, has been identified and shown not to be associated with the FinO phenotype.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D009154:Mutation; D011815:R Factors; D014158:Transcription, Genetic
D004251:DNA Transposable Elements