Alpha 2 adrenoceptor-mediated contraction of frog rectus abdominis muscle.
Effect of various concentrations of clonidine (1.875 X 10(-11) to 1.875 X 10(-6) M) was investigated on rectus abdominis muscle of frog. In lower concentrations it neither had any effect per se nor did it alter the contractile effect of acetylcholine. However, a higher concentration of clonidine (1.875 X 10(-6) M) evoked characteristic slow but sustained contractions. Such an effect was not observed with epinephrine, norepinephrine or isoprenaline. Clonidine-induced sustained contractions were resistant to d-tubocurarine (2.545 X 10(-9) M) but susceptible to blockade by yohimbine (5.116 X 10(-8) M), an alpha 2 antagonist, and verapamil (2.04 X 10(-8) M), a calcium channel blocker. Probably activation of post-synaptic alpha 2 adrenoceptors by clonidine was responsible for the observed effect. This is further supported by the observation that guanfacine, a more specific alpha 2 adrenoceptor agonist, also evoked identical, slow and sustained contractions which could be readily reversed by yohimbine.
D000009:Abdominal Muscles / Q000187:drug effects*; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D005260:Female; D016316:Guanfacine; D006146:Guanidines / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D010648:Phenylacetates / Q000494:pharmacology; D011898:Ranidae; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects* / Q000502:physiology; D014403:Tubocurarine / Q000494:pharmacology; D015016:Yohimbine / Q000494:pharmacology
D006146:Guanidines; D010648:Phenylacetates; D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; D016316:Guanfacine; D000109:Acetylcholine; D014403:Tubocurarine