Photodynamic inactivation of bladder cancer cells (MGH-U1) sensitized with acridine orange and irradiated by argon laser.
Photodynamic inactivation of bladder cancer cells (MGH-U1) was investigated in order to apply laser therapy to the treatment of bladder cancer. After stained with acridine orange (AO), the material cells were irradiated with argon laser. After 24 hr incubation the survival was counted with a hemocytometer. The number of stained cells showed a less than 5% decrease and the number of irradiated (for 15 min) unstained cells showed no decrease compared with untreated cells. Participation of singlet oxygen process in inactivation of MGH-U1 cells was confirmed by the use of D2O and NaN3. The result shows that argon laser at the low intensity and with short irradiation time has a sufficient cytocydal effect, suggesting the usefulness of photodynamic inactivation of argon laser with topical use of acridine orange in the treatment of bladder cancer.
D000165:Acridine Orange / Q000627:therapeutic use; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003903:Deuterium / Q000494:pharmacology; D017666:Deuterium Oxide; D006801:Humans; D053685:Laser Therapy; D010778:Photochemotherapy; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000628:therapy*; D014867:Water / Q000494:pharmacology
D014867:Water; D003903:Deuterium; D000165:Acridine Orange; D017666:Deuterium Oxide