[3H]prazosin binding to central nervous system regions of male and female rats.
Membranes were obtained from the pituitary gland and 13 cerebral regions of male rats and of females during two endocrinological states (estrous and diestrous-1), and assayed for alpha 1-receptor binding, using [3H]prazosin. At two [3H]prazosin concentrations, one near its binding dissociation constant and the other at a value at which the plateau was reached in the binding saturation curve (0.2 and 1.2 nM respectively), [3H]prazosin binding was highest to the frontal cortex, lowest to the spinal cord, and showed no differences in any region under any of the hormonal states tested. Results are discussed in relation to previously reported hormonal effects on alpha 1-receptors.
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D004971:Estrus; D005260:Female; D008297:Male; D011224:Prazosin / Q000378:metabolism*; D011247:Pregnancy; D011799:Quinazolines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D012737:Sex Factors
D011799:Quinazolines; D011942:Receptors, Adrenergic, alpha; D011224:Prazosin