Asparagine as precursor for transmitter aspartate in corticostriatal fibres.
The role of asparagine as precursor for the neurotransmitter aspartate was investigated in rat striatum in vitro. 14C-asparagine incubated with striatal slices is converted to a great extent to 14C-aspartate which is released in a calcium-dependent manner by high KCl. Furthermore, a frontoparietal cortex ablation of two weeks produces a decrease of more than 70% in the striatal release of newly synthetized 14C-aspartate, whereas the striatal GABA release is unaffected. This suggests that asparagine is a possible precursor in vitro for transmitter aspartate in the striatum. This reaction is dependent on intact corticostriatal fibres.
D000818:Animals; D001216:Asparagine / Q000378:metabolism*; D001224:Aspartic Acid / Q000096:biosynthesis*; D002250:Carbon Radioisotopes; D003342:Corpus Striatum / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis
D002250:Carbon Radioisotopes; D018377:Neurotransmitter Agents; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D001216:Asparagine