Different interactions of steric isomers of hexobarbital to muscarinic agonist and antagonist binding sites in brain.
The interaction between the racemate, or the steric isomers of hexobarbital, and the binding of [3H]oxotremorine or [3H]quinuclidinylbenzilate to muscarinic binding sites was studied in different parts of the rat brain. In concentrations clearly within those reached when inducing anesthesia, hexobarbital displaced both the agonist and the antagonist from their binding sites. (-)-R-hexobarbital, which is least potent as an anesthetic, was most potent as a displacer. In most brain regions, the agonist was more readily displaced than the antagonist by both isomers of hexobarbital.
D000768:Anesthesia, General; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D006591:Hexobarbital / Q000378:metabolism*; D008297:Male; D010095:Oxotremorine / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013237:Stereoisomerism
D011963:Receptors, GABA-A; D011976:Receptors, Muscarinic; D010095:Oxotremorine; D011813:Quinuclidinyl Benzilate; D006591:Hexobarbital