Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
Poly-A+ mRNA from Xenopus laevis oocytes, partially enriched for r-protein coding capacity has been used as starting material for preparing a cDNA bank in plasmid pBR322. The clones containing sequences specific for r-proteins have been selected by translation of the complementary mRNAs. Clones for six different r-proteins have been identified and utilized as probes for studying their genomic organization. Two gene copies per haploid genome were found for r-proteins L1, L14, S19, and four-five for protein S1, S8 and L32. Moreover a population polymorphism has been observed for the genomic regions containing sequences for r-protein S1, S8 and L14.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000235:genetics*
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D011061:Poly A; D004247:DNA