Degradation of DNA by silicic acid.
S1 nuclease hydrolysis and hydroxyapatite chromatography were used to study the effect of silicic acid on DNA. Native calf thymus DNA was incubated with increasing concentrations of silicic acid (DNA nucleotide/silicic acid molar ratios of 1:0.25, 1:0.5 and 1:1) and subjected to S1 nuclease hydrolysis. An increasing degree of DNA degradation was seen suggesting a destabilization of the secondary structure. A decrease in melting temperature was also observed. Hydroxyapatite chromatography indicated that incubation at the molar ratio of 1:1 resulted in denaturation and degradation of DNA.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004247:DNA; D017886:Durapatite; D004720:Endonucleases; D006868:Hydrolysis; D006882:Hydroxyapatites; D009691:Nucleic Acid Denaturation; D012824:Silicic Acid; D012822:Silicon Dioxide; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013950:Thymus Gland
D006882:Hydroxyapatites; D012824:Silicic Acid; D012822:Silicon Dioxide; D004247:DNA; D017886:Durapatite; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases