title: An improved method for the estimation of suramin in plasma and trypanosome samples.
abstract: An improved method for the chemical estimation of suramin is described in which the aromatic amines released from the drug by acid hydrolysis are diazotised and then coupled to N-(1-naphthyl)-ethylenediamine to form a pink coloured product (EmM545nm267 /+- 5) Provided certain precautions are followed, the assay method is highly reproducible (+/- 2%) and sufficiently sensitive to measure 2.5 nmol; suramin in mixtures with plasma (0.5 ml) or trypanosomes (200 mg wet wt.).
mesh_terms: D000818:Animals; D006868:Hydrolysis; D008722:Methods; D051381:Rats; D013498:Suramin / Q000032:analysis* / Q000097:blood; D014346:Trypanosoma brucei brucei / Q000032:analysis*; D014353:Trypanosomiasis, African / Q000097:blood*
chemical_list: D013498:Suramin