Isolation of a polyomavirus with an insertion of foreign DNA in the early gene promoter region.
We have isolated a polyomavirus with 134 base pairs of foreign DNA between the origin of replication and the early promoter. The insertion reduces the infectivity of the virus by interfering with events required for the initiation of infection. mRNA transcripts from the early region exhibit a marked heterogeneity of 5' termini.
D001483:Base Sequence; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics*; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral