Induction and long-term maintenance of Thy-1 positive T lymphocytes: derivation from continuous bone marrow cultures.
In the present study we investigated the presence of T-lymphocyte progenitors in the long-term murine bone marrow culture system described by Dexter: mature Thy-1 antigen-bearing T lymphocytes are lost in these cultures after a few days. By culturing nonadherent cells from such cultures in the presence of a supernatant of concanavalin A-stimulated spleen cells, a source of T-cell growth factor, we found that Thy-1 positive blast cells proliferated together with a second population of Thy-1 negative cells. These two populations of cells have been maintained in long-term in vitro cultures by passaging the cells in fresh conditioned medium at regular intervals. Moreover, we have been able to establish pure cultures of the Thy-1 bearing blast cells after separating them from the non-T cells using their adherence property to plastic surfaces. Long-term cultures of T lymphocytes can thus be established from long-term marrow cultures as well as from the spleen, thymus or fresh bone marrow.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D008565:Membrane Proteins; D018800:Thy-1 Antigens