title: Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.
abstract: Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human-mouse hybrid cell line. These libraries were constructed in the new expression vectors Homer V and Homer VI. The collection of cosmids isolated is enriched for unique sequence DNA and only a few of the cosmids contain the tandemly repeated sequences which constitute a major portion of the Y chromosome. Three cosmids have been studied in detail. One cosmid shows extensive homology over at least 20 kb with the long arm of the X chromosome; this homology is outside the predicted homology region required for sex chromosome pairing. The other two clones contain unique sequences specific to the Y chromosome and both map to the heterochromatic region of the Y chromosome long arm.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000502:physiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D006570:Heterochromatin / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D008297:Male; D051379:Mice; D010957:Plasmids; D014998:Y Chromosome
chemical_list: D002843:Chromatin; D006570:Heterochromatin; D004262:DNA Restriction Enzymes