L-glutamate transport in renal plasma membrane vesicles.
This review describes the uptake of L-glutamate by well-characterized preparations of renal brush border (liminal) and baso-lateral membrane vesicles derived from the plasma membrane of the polar proximal tubular cell. L-glutamate is taken up against its concentration gradient, from both sides, by co-transport systems in which the movement of the amino acid into the cell is coupled to the influx of Na+ and efflux of K+ down their respective electrochemical gradients. The presence of these ion gradient-energized systems, specific for L-glutamate, may account for the exceedingly high intracellular concentration of their metabolically important amino acid in the renal tubule.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002414:Cations, Monovalent; D002462:Cell Membrane / Q000378:metabolism*; D004734:Energy Metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006863:Hydrogen-Ion Concentration; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007700:Kinetics; D008564:Membrane Potentials; D008871:Microvilli / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
D000596:Amino Acids; D002414:Cations, Monovalent; D005971:Glutamates; D014634:Valinomycin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D018698:Glutamic Acid; D012964:Sodium; D011188:Potassium