title: The frog interosseal muscle fiber as a new model for patch clamp studies of chemosensitive- and voltage-sensitive ion channels: actions of acetylcholine and batrachotoxin.
abstract: The patch clamp technique was used to record the currents flowing through single ion channels in isolated frog muscle fibers. The majority of the acetylcholine (ACh)-activated channels had a conductance of 32 pS, although 20 pS channels were also occasionally observed. Lifetimes of ACh-activated channels increased with the transmembrane potential in the range from - 30 mV to - 105 mV. In these same fibers we also observed channels which were activated by low concentrations of batrachotoxin (BTX; 10 nM). These channels, presumed to be Na channels, had a conductance of 19 pS and opened at potentials at which Na channels would not normally open. A notable feature of these BTX-activated channels was that they opened and closed repeatedly. Therefore, it appears that the toxin, in addition to activating Na channels, also blocks the inactivation process. The physiological properties of these channels reveal significant differences between the ion channels of tissue-cultured and mature tissues.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001001:Anura; D001496:Batrachotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D009132:Muscles / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology*; D011978:Receptors, Nicotinic / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
chemical_list: D001496:Batrachotoxins; D007473:Ion Channels; D009498:Neurotoxins; D011978:Receptors, Nicotinic; D012964:Sodium; D000109:Acetylcholine