title: Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017.
abstract: We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.
mesh_terms: D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D006460:Hemolysin Proteins