Separation of human and Chinese hamster arylsulfatases A and B by two-directional polyacrylamide gel electrophoresis.
Two-directional or 'mirrow' polyacrylamide gel electrophoresis permits one simultaneously to separate and detect human and Chinese hamster arylsulfatases A and B. Such a separation was not possible in conventional one-dimensional polyacrylamide gel systems. The amount of each enzyme present could be quantitated by scanning at 515 nm after incubation of the gel in nitrocatechol sulfate. Direct comparisons showed that this system is at least as sensitive as a cellogel-methylumbelliferone sulfate method. Some advantages of the polyacrylamide method are described.
D000818:Animals; D002460:Cell Line; D002553:Cerebroside-Sulfatase / Q000302:isolation & purification*; D002803:Chondro-4-Sulfatase / Q000302:isolation & purification*; D006224:Cricetinae; D003412:Cricetulus; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005347:Fibroblasts; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D010053:Ovary; D013429:Sulfatases / Q000302:isolation & purification*
D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase