title: Chemistry of proton abstraction by glycolytic enzymes (aldolase, isomerases and pyruvate kinase).
abstract: Intermediates have been synthesized that are rapidly utilized by triose phosphate isomerase, yeast aldolase and pyruvate kinase. In each case the compounds have the properties of an enol expected for a stepwise proton transfer mechanism. Apparently the apparatus required for doing this chemistry is sufficiently unique for a large measure of structural homology to have been imposed upon the enzymes of this class during evolution.
mesh_terms: D005075:Biological Evolution; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D011522:Protons; D011770:Pyruvate Kinase / Q000378:metabolism*; D014305:Triose-Phosphate Isomerase / Q000378:metabolism*
chemical_list: D011522:Protons; D011770:Pyruvate Kinase; D005634:Fructose-Bisphosphate Aldolase; D002238:Carbohydrate Epimerases; D014305:Triose-Phosphate Isomerase