Evidence for the presence of fatty acid methyl esters in human hepatoma tissue from a patient with hypercholesterolemia.
The lipid composition of a human hepatoma tissue (liver cell carcinoma) was examined by thin-layer chromatography (TLC), and an unusual lipid spot was found in a large quantity. It was identified as consisting of fatty acid methyl esters by TLC and gas chromatography-mass spectrometry techniques. In the fatty acid methyl ester fraction, oleate (18:1) and palmitate (16:0) were dominant. Furthermore, very unusual fatty acid methyl esters, such as methyl 2-methyl oleate, were detected. These unusual lipids could be detected neither in cirrhotic lesions of the host liver nor in normal control tissues.
D006528:Carcinoma, Hepatocellular / Q000032:analysis* / Q000150:complications; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004952:Esters / Q000302:isolation & purification; D005227:Fatty Acids / Q000302:isolation & purification*; D006511:Hepatitis B Antigens / Q000032:analysis; D006801:Humans; D006937:Hypercholesterolemia / Q000150:complications*; D008103:Liver Cirrhosis / Q000150:complications*; D008113:Liver Neoplasms / Q000032:analysis* / Q000150:complications; D013058:Mass Spectrometry; D009829:Oleic Acids / Q000302:isolation & purification; D010168:Palmitates / Q000302:isolation & purification
D004952:Esters; D005227:Fatty Acids; D006511:Hepatitis B Antigens; D009829:Oleic Acids; D010168:Palmitates; C005576:methyl oleate; C019012:methyl palmitate