title: Phosphate uptake by kidney epithelial (LLC-PK1) cells.
abstract: Phosphate uptake by the cultured kidney epithelial cell (LLC-PK1) was studied. The uptake was Na+ dependent, saturable with respect to phosphate and Na+, and energy dependent. The characteristics of the cell uptake system resembled the properties of phosphate transport in the kidney. Parathyroid hormone, dibutyryl cyclic AMP, and forskolin decreased Na+-dependent phosphate uptake. These agonists did not affect Na+-dependent alpha-methylglucoside uptake. Vasopressin and isoproterenol, which do not affect renal phosphate transport, did not inhibit phosphate uptake by the cell. These findings suggest that the cultured cell system may be a useful experimental model for studies of renal phosphate transport and its regulation.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism*; D012964:Sodium / Q000502:physiology; D013552:Swine; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D004224:Diterpenes; D010281:Parathyroid Hormone; D010710:Phosphates; D014667:Vasopressins; D005576:Colforsin; D003994:Bucladesine; D012964:Sodium; D000262:Adenylyl Cyclases; D007545:Isoproterenol