Establishment of a human diploid cell strain.
A new human diploid fibroblast-like cell strain has been established from dermal tissue of a 2 months old female embryo. This cell strain showed diploid karyotype and has a finite life span of 95-100 generations. No spontaneous transformation was observed during the whole life span in vitro. This cell strain is susceptible to SV40 infection and can be transformed easily by it.
D002460:Cell Line; D002470:Cell Survival; D002472:Cell Transformation, Viral; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D013539:Simian virus 40; D012867:Skin / Q000166:cytology / Q000196:embryology