A comparison of three methods of hydrolysis for estrogen conjugates.
The efficiencies for estrogen conjugate hydrolysis were compared between enzyme hydrolysis, acid solvolysis and a new method, ammonolysis. Samples included: 1) crystalline 1,3,5(10)-estratriene-3, 17 beta-diol disulfate (estradiol 3,17-disulfate), 2) squirrel monkey urine collected following an intravenous injection of [2,4,6,7-H] 1,3,5(10)-estratriene-3,17 beta-diol (estradiol) and 3) a pool of human pregnancy urine. Ammonolysis demonstrated a significant increase over the other techniques in "free" estrogen yields, specifically, from estradiol 3,17-disulfate.
D000641:Ammonia; D000818:Animals; D001192:Arylsulfatases; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D004966:Estrogens, Conjugated (USP) / Q000032:analysis* / Q000652:urine; D005260:Female; D005966:Glucuronidase; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011247:Pregnancy; D012453:Saimiri / Q000652:urine; D012964:Sodium
D004966:Estrogens, Conjugated (USP); C045407:estradiol 3,17-disulfate; D004958:Estradiol; D000641:Ammonia; D012964:Sodium; D001192:Arylsulfatases; D005966:Glucuronidase