S1-hypersensitive sites in eukaryotic promoter regions.
We have examined by fine mapping the S1 nuclease-hypersensitivity of the 5' flanking regions of the human beta-globin and rat preproinsulin II genes and of the SV40 origin/enhancer region. In all cases S1-hypersensitive sites are located in known or presumed promoter/regulatory regions. Though a consensus DNA sequence is not evident, all of these sites reside in predominantly homopurine-homopyrimidine stretches. The alternate (non-B) DNA structure which is revealed by the enzymatic probe is a sequence-dependent feature of a short stretch of DNA, which is retained upon transplantation into a foreign environment. The alternate structure exhibits S1-nicking patterns uniquely different from those associated with the presence of Z-DNA.
D000818:Animals; D004262:DNA Restriction Enzymes; D004720:Endonucleases / Q000378:metabolism*; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D007328:Insulin; D010957:Plasmids; D011384:Proinsulin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013379:Substrate Specificity
D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D005914:Globins; D011384:Proinsulin; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases