High-resolution hydroxyapatite chromatography of proteins.
Hydroxyapatite chromatography has been used to separate all five isozymes of lactic dehydrogenase, six enzymatically active forms of bovine pancreatic DNase I, and a standard protein mixture. The proteins were eluted with a linear gradient of sodium phosphate. Enzyme activity recoveries were greater than 90%. Packing materials were obtained from commercial fine-particle-sized hydroxyapatite (DNA grade Bio-Gel HTP) by an elutriation procedure. Long columns packed with small crystals were run under low pressure at acceptable flow rates, and were used over prolonged periods.
D001407:Bacillus / Q000201:enzymology; D002845:Chromatography / Q000379:methods*; D003850:Deoxyribonuclease I / Q000032:analysis; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D006882:Hydroxyapatites; D007527:Isoenzymes; D011506:Proteins; D017886:Durapatite; D007770:L-Lactate Dehydrogenase; D003850:Deoxyribonuclease I