title: Proton-induced membrane fusion. Role of phospholipid composition and protein-mediated intermembrane contact.
abstract: Glycolipid-phospholipid vesicles containing phosphatidate and phosphatidylethanolamine were found to undergo proton-induced fusion upon acidification of the suspending medium from pH 7.4 to pH 6.5 or lower, as determined by an assay for lipid intermixing based on fluorescence resonance energy transfer. Lectin-mediated contact between the vesicles was required for fusion. Incorporation of phosphatidylcholine in the vesicles inhibited proton-induced fusion. Vesicles in which phosphatidate was replaced by phosphatidylserine underwent fusion only when pH was reduced below 4.5, while no significant fusion occurred (pH greater than or equal to 3.5) when the anionic phospholipid was phosphatidylinositol. It is suggested that partial protonation of the polar headgroup of phosphatidate and phosphatidylserine, respectively, causes a sufficient reduction in the polarity and hydration of the vesicle surface to trigger fusion at sites of intermembrane contact.
mesh_terms: D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000502:physiology*; D010712:Phosphatidic Acids / Q000502:physiology; D010713:Phosphatidylcholines / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000502:physiology; D010716:Phosphatidylinositols / Q000502:physiology; D010718:Phosphatidylserines / Q000502:physiology; D010743:Phospholipids / Q000502:physiology*; D011522:Protons
chemical_list: D008081:Liposomes; D008565:Membrane Proteins; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D011522:Protons