Identification of sialyl and galactosyl transferase activities in calf vitreous hyalocytes.
Sialyl and galactosyl transferase activities are demonstrated in calf vitreous hyalocytes. For study of sialyl transferase activity, a partially purified vitreous preparation (collagen and hyaluronic acid removed), and bovine submaxillary mucin were treated with an insolubilized neuraminidase before use acceptor of radioactivity from CMP-[3H]-N-acetylneuraminic acid (CMP-[3H]-NAN). For study of galactosyl transferase activity the vitreous preparation was treated first with insolubilized neuraminidase and then with an insolubilized beta-galactosidase before use as acceptor of radioactivity from UDP-[3H]-galactose (UDP-[3H]-gal). Galactosyl transferase requires a divalent metal ion for optimal activity, and the reactions catalyzed by each enzyme are dependent upon pH, time of incubation and concentration of enzyme and/or acceptor.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D003016:Clostridium perfringens / Q000201:enzymology; D003470:Culture Media; D005700:Galactosyltransferases / Q000302:isolation & purification*; D066298:In Vitro Techniques; D008264:Macrophages / Q000201:enzymology*; D009077:Mucins; D009439:Neuraminidase; D012799:Sialyltransferases / Q000302:isolation & purification*; D012995:Solubility; D013363:Submandibular Gland / Q000378:metabolism; D014166:Transferases / Q000302:isolation & purification*; D014316:Tritium; D014822:Vitreous Body / Q000166:cytology*; D001616:beta-Galactosidase
D003470:Culture Media; D009077:Mucins; D014316:Tritium; D014166:Transferases; D005700:Galactosyltransferases; D012799:Sialyltransferases; D009439:Neuraminidase; D001616:beta-Galactosidase