Family studies of the genetic abnormality in transthyretin (prealbumin) in Portuguese patients with familial amyloidotic polyneuropathy.
Amyloid deposits in several heredofamilial forms of amyloidosis are chemically related to transthyretin (TTR, the protein usually referred to as prealbumin). A genetically abnormal TTR may be involved. Studies were conducted on TTR isolated from sera of patients with familial amyloidotic polyneuropathy (FAP), and on amyloid fibril protein (AFp) isolated from tissues of two Portuguese patients who died with FAP. AFp, purified by affinity chromatography on retinol-binding protein (RBP), resembled plasma TTR in forming a stable tetrameric structure, and in its binding affinities for both thyroxine and RBP. Purified AFp was found to comprise a TTR variant with a methionine for valine substitution at position 30. This conclusion was based upon studies that included: (i) comparative peptide mapping by reverse-phase high-performance liquid chromatography after trypsin digestion; (ii) cyanogen bromide (CNBr) cleavage studies; and (iii) amino acid microsequence analysis of selected tryptic and CNBr peptides. The variant TTR was also found to be present in serum samples from FAP patients, along with larger amounts of normal TTR. An effective, small-scale procedure was developed to determine whether or not the variant TTR was present in the plasma of an individual subject. This procedure involved isolation of TTR by affinity chromatography on RBP, followed by CNBr cleavage, and analysis for the presence of specific aberrant CNBr peptides. Studies with six kindreds, including 21 asymptomatic children of 6 patients with FAP, showed that the "abnormal" TTR can be detected and used as a preclinical marker of the disease in affected children of patients with FAP. It is likely that the variant TTR represents a point mutation within the TTR structural gene, and that the normal and mutant genes act as co-dominant alleles at a single locus in FAP. The distribution of the mutant TTR within the six families was consistent with the autosomal dominant mode of inheritance of FAP. The mutant TTR apparently selectively deposits in tissues as the amyloid characteristic of the disease.
D000293:Adolescent; D000328:Adult; D000686:Amyloidosis / Q000097:blood / Q000235:genetics*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002648:Child; D003488:Cyanogen Bromide; D005260:Female; D006801:Humans; D007668:Kidney / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D010455:Peptides / Q000302:isolation & purification; D010523:Peripheral Nervous System Diseases / Q000235:genetics*; D011174:Portugal; D011228:Prealbumin / Q000097:blood / Q000235:genetics*; D000685:Serum Amyloid A Protein / Q000302:isolation & purification; D014357:Trypsin
D010455:Peptides; D011228:Prealbumin; D000685:Serum Amyloid A Protein; D014357:Trypsin; D003488:Cyanogen Bromide