[The influence of aging, post-mortem delay until isolation of the tissue and duration of agony on some glycolytic enzymes in human autoptic brain tissue (author's transl)].
The influence of aging and pre- and post-mortem conditions on the activities of glycolytic enzymes and of the ATPases was determined in samples of autoptic human cerebral cortex and putamen. These results were compared with results obtained from an aging collective of rats (four groups ranging from 20 to 120 weeks of age). The results revealed an interlinked significant age-related increase in soluble hexokinase (HK) and a significant decrease in phosphofructokinase (PFK) activity in human autoptic tissue, whereas no significant age-dependent differences could be observed in rat brain. Subdivision of the cases according to duration of agony and other pre-mortem conditions revealed reduced variance and therefore statistically more significant PFK age-dependence in cases with a short agony. All other cases are not dependent on age. In addition to the pre- and post-mortem effects, the age-dependence of PFK and soluble HK can be clearly demonstrated in human autoptic brain tissue.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D000375:Aging; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D006593:Hexokinase / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010146:Pain / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011180:Postmortem Changes; D011699:Putamen / Q000201:enzymology; D051381:Rats
D000255:Adenosine Triphosphate; D006593:Hexokinase; D010732:Phosphofructokinase-1; D000251:Adenosine Triphosphatases
Der Einfluss von Alter, Zeitspanne bis zur postmortalen Isolierung des Gewebes sowie der Agonie auf einige glycolytische Enzyme in autoptischen Gehirnproben des Menschen.