Resistance to Trypanosoma brucei in mice following inoculation of NTG-treated trypanosomes.
The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (NTG) was tested as an attenuating agent for Trypanosoma brucei. Parasites were treated with 4 mcg/ml NTG and injected into mice at intervals of 7-14 days. Those mice surviving four injections of NTG-treated trypanosomes were later challenged and showed a 100% resistance to infection. A corresponding group of control mice showed a 6% resistance to infection.
D000818:Animals; D005260:Female; D007113:Immunity, Innate; D007114:Immunization; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D051379:Mice; D014346:Trypanosoma brucei brucei / Q000187:drug effects; D014353:Trypanosomiasis, African / Q000276:immunology* / Q000662:veterinary*
D008769:Methylnitronitrosoguanidine