[Chemico-enzymatic synthesis of an oligonucleotide template for an analog of ACTH (4-10) fragment].
Six oligodeoxyribonucleotides ranging from 9-mer to 13-mer were synthesized in solution by the phosphotriester approach and enzymatically joined by T4 DNA ligase. The obtained double-stranded DNA (32 b.p.) with protruding 5'-ends corresponding to the recognition sites for restrictases EcoRI and BamHI represents an oligonucleotide template coding for the modified amino acid sequence 4-10 of the adrenocorticotropic hormone, [Pro8,Gly9,Pro10]ACTH-(4-10).
D000324:Adrenocorticotropic Hormone / Q000138:chemical synthesis* / Q000235:genetics; D001483:Base Sequence; D005818:Genetic Engineering; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000235:genetics; D013698:Templates, Genetic
D009841:Oligonucleotides; D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone; C011394:ACTH (4-10)
Khimiko-fermentativnyĭ sintez oligonucleotidnoĭ matritsy analoga fragmenta 4-10 adrenokortikotropnogo gormona.