Light element abundance in brain nuclei using energy dispersive x-ray analysis and atomic absorption spectrophotometry.
A comparative study of light element (congruent to ion) abundance in isolated brain nuclei, using atomic absorption (AA) spectrophotometry and the scanning electron microscope mode of energy dispersive x-ray analysis (EDXA), demonstrated similar statistical trends in the abundance of Na, Mg, and K at 0 time and at 4 min of total brain ischemia. The obtained correlation strengthens the validity of the comparison of quantitative and semi-quantitative procedures. The EDXA technique represents a new approach to the study of ionic shifts in brain ischemia that may prove useful in other areas of brain research as well.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism; D004577:Electron Probe Microanalysis; D005260:Female; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013054:Spectrophotometry, Atomic
D012964:Sodium; D008274:Magnesium; D011188:Potassium