title: Physical and functional mapping of rat-liver mitochondrial DNA.
abstract: Rat-liver mitochondrial DNA (mtDNA) contains 2 cleavage sites of the restriction endonuclease Xbal. The molecular sizes of restriction fragments are 6.6 X 10(6) and 3.7 X 10(6) D. The results of partial cleavage of mtDNA with EcoRI allow the fragment F (0.32 X 10(6) D) to be localized in the sequence ABCEGFHDA. The functional map of mtDNA is constructed for two genes of the ATP-ase mRNAs from rat-liver mitochondria. Molecular hybridization shows that the ATPase genes are located in fragment B and in the GEHD area of mtDNA EcoRI cleavage.
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D008297:Male; D008930:Mitochondria, Liver / Q000032:analysis*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats
chemical_list: D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D011061:Poly A; D004262:DNA Restriction Enzymes; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific; D000251:Adenosine Triphosphatases