[Detection of anaerobes by direct immunofluorescence on clinical samples : evaluation of fluoretec F and M (author's transl)].
Immunofluorescence is a method allowing rapid identification of bacteria. The authors study two reagents. Fluoretec F and M, respectively detecting bacteroidis fragilis and bacteroidis melaninogenicus. These reactions were carried out directly on the clinical specimens. After a study carried out with 132 clinical specimens, by comparison with the usual techniques for isolation and identification, the following results were seen : Fluoretec F showed 89 p. cent concordance, 0.8 p. cent false negatives and 3 p. cent false positives. In 7.2 p. cent of cases, the type of discordance could not be determined. Fluoretec M showed 87.8 p, cent concordance, 6.1 p. cent false negatives, 0.8 p. cent false positives and in 5.3. p. cent of cases, the discordance could not be specified. Application of direct immunofluorescence for these two specific tests allowed rapid orientation of diagnosis and allowed evaluation of reliability of culture techniques.
D000693:Anaerobiosis; D001439:Bacteroides / Q000378:metabolism*; D001441:Bacteroides fragilis / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008660:Metabolism; D009930:Organic Chemicals; D001443:Prevotella melaninogenica / Q000378:metabolism; D013045:Species Specificity
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C032685:fluoretec M; C032686:Fluoretec F
Détection des anaérobies par immunofluorescence directe sur les prélèvements cliniques : évaluation des fluoretec F et M.