Mode of degradation of plasmid DNA with ozone.
The ozonization of pBR322 closed circular DNA showed the conversion to open circular DNA. The damaged site was investigated by restriction mapping. The results showed the damage and subsequent cleavage of the DNA strand of ccDNA by ozonization may occur at the region sensitive to nuclease S1.
D000818:Animals; D002417:Cattle; D004247:DNA; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D010126:Ozone; D010957:Plasmids; D013950:Thymus Gland
D004269:DNA, Bacterial; D010126:Ozone; D004247:DNA; D004262:DNA Restriction Enzymes