Lack of a direct role for cyclic AMP in parathyrin action on phosphate reabsorption by the kidney.
Isolated chick kidney proximal tubule cells have been used in a study of the mechanism by which PTH inhibits Na+-dependent Pi transport in the kidney. Treatment with PTH inhibits Pi uptake by the cells by 13% and stimulates cyclic AMP production by 77%. Forskolin, a potent activator of adenyl cyclase, brought about an 11-fold stimulation of cyclic AMP production by the cells, but in contrast to PTH, the drug had no effect on Na+-dependent Pi uptake. These results provide evidence that PTH action on phosphate transport is not mediated by cyclic AMP.
D000042:Absorption; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002645:Chickens; D005576:Colforsin; D000242:Cyclic AMP / Q000502:physiology*; D004224:Diterpenes / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism*
D004224:Diterpenes; D010281:Parathyroid Hormone; D010710:Phosphates; D005576:Colforsin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases