Metabolism of sulfanilamide and related drugs in isolated rat and rabbit liver cells.
Parenchymal and nonparenchymal cells were isolated from rat and rabbit liver by perfusion with collagenase. Pure parenchymal cells were obtained by centrifugation at low speed and nonparenchymal cells were purified by treatment with pronase. Suspensions of parenchymal cells from rat liver metabolized sulfamethazine, sulfanilamide, and p-aminobenzoic acid at rates of 39.0, 75.8, and 173.2 pmol/min/10(6) cells, respectively, but did not metabolize sulfadiazine. Rabbit liver parenchymal cells gave somewhat higher activities and also metabolized sulfadiazine. Nonparenchymal cells from both rat and rabbit liver failed to metabolize any of the compounds.
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D000107:Acetylation; D000818:Animals; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013411:Sulfadiazine / Q000378:metabolism; D013418:Sulfamethazine / Q000378:metabolism; D013424:Sulfanilamides / Q000378:metabolism*; D013997:Time Factors
D013424:Sulfanilamides; D013411:Sulfadiazine; D013418:Sulfamethazine; D010129:4-Aminobenzoic Acid