Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.
Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells. We find one loop for each of the tandemly repeated histone gene clusters. The attachment site is localized in the A + T rich H1-H3 spacer on a 657 bp fragment. In the cluster of the hsp70 heat-shock genes, in both control and heat-shocked cells, we find two attachment sites in close proximity upstream of regulatory elements. The transcribed sequences are not associated with the nuclear scaffold in control or in heat-shocked cells. A family of attachment sites related by hybridization to those of the hsp70 genes was discovered.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster; D005796:Genes; D006657:Histones / Q000235:genetics* / Q000302:isolation & purification
D002843:Chromatin; D006657:Histones; D004247:DNA; D004262:DNA Restriction Enzymes