Deletion of plasmid sequences during Saccharomyces cerevisiae transformation.
Saccharomyces cerevisiae was transformed with DNA by the lithium acetate method. Mutation of nonselected markers on the transforming vector was observed at a frequency several orders of magnitude higher than spontaneous mutation frequencies. These mutations were shown to be deletions. Linearization of the vector before transformation stimulated deletion formation.
D001483:Base Sequence; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D005800:Genes, Fungal; D005822:Genetic Vectors; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004262:DNA Restriction Enzymes