An immunofluorescent test for faecal occult blood.
An immunological test for faecal occult blood is described, which is suitable for screening purposes. Fluorescein-labelled rabbit anti-human-haemoglobin serum is mixed with an aqueous faecal suspension, and free antibody is bound to a membrane sampler coated with human haemoglobin. The fluorescence on the membrane sampler is measured in a fluorometer, and the level of haemoglobin in the suspension is estimated. Advantages over chemical tests include absence of cross-reaction with animal haemoglobins, minimal observer error, and an adjustable sensitivity.
D003110:Colonic Neoplasms / Q000175:diagnosis; D005455:Fluorescent Antibody Technique; D005470:Fluorometry; D006801:Humans; D009780:Occult Blood; D012004:Rectal Neoplasms / Q000175:diagnosis