title: Does beta-endorphin modulate basal and dopamine-inhibited prolactin release by an action at the anterior pituitary?
abstract: Previous studies performed to elucidate an anterior pituitary site of action for beta-endorphin (B-EP) in the regulation of prolactin (PRL) release have been inconclusive. The purpose of the present study was to further investigate if B-EP could stimulate PRL release by a direct action at the anterior pituitary. Anterior pituitaries from ovariectomized rats were dispersed, and the cells were plated in Ham's F-10 medium. Following a 48-hour preincubation period, the effects of B-EP on basal PRL release and on the dopamine-inhibited PRL release were studied over a 24-hour period. Overall, B-EP at concentrations ranging from 10(-8) to 10(-6) M had no effect on basal release of PRL at 1, 3, 5 and 24 h, although a small but significant increase of 39% was observed with 10(-6) M B-EP at 1 h of incubation only. B-EP at 10(-6) M, but not at lower concentrations, significantly suppressed the inhibitory effects of 10(-6) M dopamine on PRL release at 1 h but not at longer periods of incubation. Pretreatment of the cells for 3 h with 10(-6) M naloxone antagonized the suppressive effect of B-EP on the dopamine-mediated inhibition of PRL release. Naloxone pretreatment of the cells had no effect on basal nor dopamine-inhibited release of PRL. Because of the high concentration of B-EP required to suppress the dopamine inhibition and the transitory nature of this B-EP effect, it is unlikely that the primary site of action for B-EP in regulating PRL release is at the anterior pituitary.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001481:Basal Metabolism; D002478:Cells, Cultured; D004298:Dopamine / Q000502:physiology*; D004723:Endorphins / Q000502:physiology*; D005260:Female; D009270:Naloxone / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000502:physiology*; D011388:Prolactin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin
chemical_list: D004723:Endorphins; D009270:Naloxone; D001615:beta-Endorphin; D011388:Prolactin; D004298:Dopamine