title: Impediment to replication fork movement in the terminus region of the Bacillus subtilis chromosome.
abstract: The terminus regions of the chromosomes of three strains of Bacillus subtilis 168 were radioactively labelled by supplying [3H]thymine towards the end of a round of replication. These strains lacked or contained the prophage SP beta c2. Following restriction endonuclease digestion of the purified DNA and fluorography, an SP beta c2-related perturbation of the terminus-labelling profile was observed, which was completely consistent with the previously suggested existence of an impediment to replication fork movement (terC) within a BamHI 24.8 X 10(3) base fragment (Weiss & Wake, 1983). The present data suggest that terC is located within the 11.4 X 10(3) base BamHI + SalI double-digest portion of this BamHI fragment.
mesh_terms: D001412:Bacillus subtilis / Q000648:ultrastructure*; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D005809:Genes, Regulator; D013728:Terminator Regions, Genetic
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes