Separation of human spermatozoa from a single ejaculate into various age-groups.
Ejaculated human spermatozoa were separated into various age-groups on the basis of their different specific gravity. Salt-poor 25% human serum albumin was used as a separation medium. The separation process had no detectable harmful effect on the spermatozoa. The motility and penetration abilities of the separated cell fractions were tested by means of a new method using a fragiligraph. The younger group of spermatozoa demonstrated better motility and penetration ability in comparison with the older age-groups.
D000328:Adult; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D006801:Humans; D008297:Male; D013046:Specific Gravity; D013081:Sperm Motility; D013094:Spermatozoa / Q000145:classification*