title: Guanylate cyclase activity in Escherichia coli mutants defective in adenylate cyclase.
abstract: Guanylate cyclase, which catalyzes the synthesis of guanosine 3',5'-monophosphate, has been assayed in several strains of Escherichia coli. They include wild-type cells and mutants defective in adenylate cyclase, which is responsible for the synthesis of adenosine 3',5'-phosphate. Our results demonstrate that adenylate cyclase and guanylate cyclase are two different enzymes in E. coli and suggest that the gene that encodes adenylate cyclase also plays a regulatory role in the synthesis of guanylate cyclase.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006160:Guanosine Triphosphate / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D009154:Mutation; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D008345:Manganese; D006160:Guanosine Triphosphate; D012969:Sodium Fluoride; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D006152:Cyclic GMP