Epstein-Barr virus activation of human epithelial-nasopharyngeal carcinoma hybrid cells.
The authors used indirect immunofluorescence and electron microscopic techniques to study the effects of 5-iododeoxyuridine (IUDR) and the tumor promotor 12-O-tetradecanoylphorbol-13-acetate (TPA) on the epithelial hybrid cell line (NPC-KT) derived from nasopharyngeal carcinoma. The hybrid cells contained Epstein-Barr virus (EBV)-induced early antigens at a very low level but not Epstein-Barr viral capsid antigens in spontaneous production; the hybrid cells responded significantly to both IUDR and TPA induction. The chemicals induced both early antigens and viral capsid antigens in the NPC-KT cells. Moreover, the virus particles were detected in the altered NPC-KT cells by IUDR treatment. The data suggest that the EBV-induced agents activate the EBV productive cycle in a proportion of nasopharyngeal carcinoma cells.
D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects / Q000382:microbiology*; D007065:Idoxuridine / Q000494:pharmacology; D008854:Microscopy, Electron; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects
D000957:Antigens, Viral, Tumor; D007065:Idoxuridine; D013755:Tetradecanoylphorbol Acetate