title: [Endogenous nitrogen metabolism in 15N-labeled swine. 3. Endogenous excretion of 15N- and 14C-labeled secretions following a 14C-leucine injection].
abstract: Four pigs (59-65 kg live weight) were labelled over a period of 10 days with 15N in the feeding of a fishmeal diet (1), a fishmeal diet + partly hydrolysed straw meal (2), a horse bean diet (3) and a horse bean diet + partly hydrolysed straw meal (4). After a 24-hour fasting the animals were provided with simple cannulae in the upper part of the small intestines. After a fasting period of 24 h all four pigs received a 14C-leucine injection and the cannula secretion was collected in the subsequent 24 h. After the feeding of the diets without straw meal supplement (1 and 3) there were distinct differences in the secretion in comparison with the feeding with straw meal supplements (2 and 4) despite the long fasting period (48-72 h). 14C-activity could already be detected in the TCA-precipitable fraction of the secretion after 3-6 min of the injection in 1 and 3 but only 20 to 25 min after the 14C-leucine injection in 2 and 4. The specific 14C-leucine activity of the TCA-soluble fraction of the secretion was, after the straw meal supplementation to the fish meal diet, 15 times higher 25 min after the 14C-leu-injection, 25 times higher after 70 min, 36 times after 2 h and 1.8 times after 4 h than without straw meal supplementation. For all four diets a specific correlation (r = 0.96) could be ascertained between the increase of 14C-activity/mg N in the TCA-soluble fraction and the increasing crude fibre content in the diet between 25 and 180 min after the injection. Furthermore, a distinctly decreased N-secretion/h could be ascertained (correlation coefficient r = 0.84) with the increasing crude fibre content in the diet. The influence of the crude fibre on the parameters mentioned is seen in the changed osmotic conditions in the secretion, which may be caused by the changed regulation by hormones of the gastro-intestinal tract. The atom-% 15N' in both TCA-fractions of the secretion underwent big rhythmic variations, which is explained by different ratios of the components pancreatic juice, bile, and intestinal juice.
mesh_terms: D000821:Animal Feed; D000818:Animals; D002250:Carbon Radioisotopes; D004043:Dietary Fiber / Q000008:administration & dosage; D005527:Food, Fortified; D007419:Intestinal Secretions / Q000378:metabolism*; D007930:Leucine / Q000008:administration & dosage*; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D013552:Swine / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D004043:Dietary Fiber; D009587:Nitrogen Isotopes; D007930:Leucine; D009584:Nitrogen
vernacular_title: Untersuchungen zu endogenen N-Umsatzprozessen an 15N-markierten Schweinen. 3. Mitteilung. Endogene Ausscheidung von 15N- und 14C-markierten Sekreten nach einer 14C-Leuzininjektion.