title: Polar encapsidation of adenovirus DNA: evolutionary variants reveal dispensable sequences near the left ends of Ad3 genomes.
abstract: Repeated passage of adenovirus type 3 in HeLa cells has led to a novel stock of variant genomes, characterized by deletions and substitutions of DNA sequences within the left-end 750 base pairs. This heterogeneous stock retains few if any parental genomes--the majority of variants appear viable. Analysis of viable variants with deleted sequences reveals the 182 nucleotides proximal to the left-end inverted terminal repeat (136-318 bp) are not required for Ad3 infectivity in cultured human cell lines nor for maintenance of viral DNA encapsidation polarity.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D002213:Capsid / Q000235:genetics*; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D014644:Genetic Variation; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D010957:Plasmids
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes