Oxygen radical production by horse and pig neutrophils induced by a range of crystals.
The exposure of animal neutrophils to crystals implicated in joint inflammation induces superoxide and peroxide generation in a concentration and temperature dependent fashion. Active crystals were urate, hydroxyapatite, pyrophosphate and brushite; diamond and cholesterol were inactive. Cytochalasin B increased superoxide yield after addition of brushite, and inhibitors of the PMA induced respiratory burst blocked the crystal induced response. Addition of urate to anaerobic neutrophils causes the reduction of a b-type cytochrome, a likely component of the neutrophil superoxide generating system.
D000818:Animals; D001168:Arthritis / Q000378:metabolism; D003460:Crystallization; D006073:Gout / Q000378:metabolism; D006736:Horses; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism*; D013552:Swine; D014527:Uric Acid / Q000494:pharmacology*
D013481:Superoxides; D014527:Uric Acid