title: Microwave and thermal interactions with oxidative hemolysis.
abstract: The influence of microwave radiation (2450 MHz, 3,333 pulses per second, duty factor of 0.02, and average specific absorption rate of 0.4 W/kg) on spontaneous hemolysis of human erythrocytes was examined. Cells were exposed to microwave radiation for 20 minutes at 37 degrees, 42 degrees, or 48 degrees C. Some of these cells were sensitized to oxidative damage by treatment with 1-chloro-2,4-dinitrobenzene (CDNB) and/or by coating with wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate. Microwave radiation significantly decreased spontaneous hemolysis of untreated cells at 42 degrees C but had no effect at 37 degrees or 48 degrees C. Microwave exposure significantly enhanced a CDNB membrane stabilizing effect at 42 degrees C but had no effect at 37 or 48 degrees C. At 42 degrees C, microwave exposure increased hemolysis of WGA-HRP coated cells. Cells treated with both WGA-HRP and CDNB showed no change in fragility at 42 degrees C and increased fragility at 48 degrees C without a microwave effect. The microwave effects observed appear to involve perturbation of the thermal threshold for oxidative hyperthermic hemolysis.
mesh_terms: D004137:Dinitrochlorobenzene; D004912:Erythrocytes / Q000528:radiation effects*; D006461:Hemolysis / Q000528:radiation effects*; D006358:Hot Temperature; D006801:Humans; D008872:Microwaves; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000097:blood; D013481:Superoxides / Q000097:blood
chemical_list: D004137:Dinitrochlorobenzene; D013481:Superoxides; D010544:Peroxidases