Viability of cultured peripheral blood mononuclear cells from atopic individuals.
The viability of cultured peripheral blood mononuclear cells (MNC) from atopic children was found to be subnormal. After 3 days of culture, less than 5% of cells from nonatopic children had died as judged by trypan blue exclusion tests, whereas more than 4 times as many dead cells were observed in cultures of cells from atopics with high IgE levels (greater than 400 units/ml). MNC from atopics with low or moderately increased IgE levels did not display any significantly decreased viability. Low cell viability was observed primarily in cultures from patients with atopic dermatitis, but also in cases with respiratory allergy with high IgE levels but without concomitant atopic dermatitis, there was a decreased viability. There was no evidence that low cell viability was causally related to occurrence of cytotoxic serum factors. Results of experiments where dibutyryl cyclic AMP or theophylline was included in the cultures suggested that a possible explanation for the decreased viability might be altered sensitivity of the cells to inactivation by cyclic AMP promoting substances.
D000293:Adolescent; D003994:Bucladesine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood*; D008214:Lymphocytes / Q000503:physiopathology; D009000:Monocytes / Q000166:cytology*; D013806:Theophylline / Q000494:pharmacology
D003994:Bucladesine; D013806:Theophylline