Agarose: a possible universal gel exclusion agent.
Granulated agarose gels suitable for gel exclusion chromatography of proteins of any molecular weight may now be prepared. This was made possible by the observation that agarose solutions of 16% polysaccharide may be prepared by displacing 8% agarose from solution with 8% polyethylene glycol Mr 6000. The displaced polysaccharide concentrates in a viscous mass occupying half the volume of the original carbohydrate solution. By diluting the displaced polysaccharide with hot watery solutions of electrolyte and allowing the solutions to congeal, gels of any desired concentration, ranging from low to the maximum of 16%, may be prepared.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods*; D003574:Cytochrome c Group / Q000302:isolation & purification; D006433:Hemocyanins / Q000302:isolation & purification; D008970:Molecular Weight; D010047:Ovalbumin / Q000302:isolation & purification; D012685:Sepharose
D003574:Cytochrome c Group; D010047:Ovalbumin; D012685:Sepharose; D006433:Hemocyanins