title: Surface activity of polypeptide toxins isolated from Anemonia sulcata.
abstract: Circular dichroism spectra and surface activities are reported for toxins II and III isolated from Anemonia sulcata. Toxin II is highly surface active and as a result possesses a synergistic effect with phospholipase A2. In complete contrast, toxin III lacks detectable surface activity. The presence of sodium dodecyl sulfate (2.5 mg.ml-1) failed to trigger large conformation changes in both toxins II and III. From a comparison of the sequences of toxins II and III it is suggested that the hydrophobic region 17-27 is responsible for the surface activity of toxin II.
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D003064:Cnidarian Venoms / Q000302:isolation & purification*; D004357:Drug Synergism; D007700:Kinetics; D008081:Liposomes; D008555:Melitten; D010179:Pancreas / Q000201:enzymology; D010455:Peptides / Q000302:isolation & purification*; D010713:Phosphatidylcholines; D010741:Phospholipases A; D054467:Phospholipases A2; D012615:Sea Anemones / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013499:Surface Properties
chemical_list: D003064:Cnidarian Venoms; D008081:Liposomes; D010455:Peptides; D010713:Phosphatidylcholines; D008555:Melitten; D012967:Sodium Dodecyl Sulfate; C028800:toxin II (Anemonia sulcata); C032104:toxin III (Anemonia sulcata); D010741:Phospholipases A; D054467:Phospholipases A2