Ovulation in the marmoset monkey: endocrinology, prediction and detection.
Circulating progesterone, oestrogens and LH were measured in female marmosets (Callithrix jacchus) over the periovulatory period. Progesterone concentrations increased in all animals within 1 day of the estimated day of ovulation, confirming the usefulness of this hormone for retrospective detection of ovulation. Oestradiol-17 beta and LH both showed a preovulatory rise, but due to the large quantity of plasma required (oestradiol: 0.2 ml) and the length of time taken for the assay (LH: 2-3 days), measurement of these hormones is not practical for the prediction of ovulation. There were no preovulatory changes in unconjugated oestrone, but a rise in total (i.e. conjugated plus unconjugated) oestrone was used to time the collection of recently ovulated oocytes. Levels of oestrone-3-sulphate showed an increase at least 1 day before the expected day of ovulation in four out of five animals. This preovulatory rise can be measured easily by a rapid direct assay, thereby providing a practical method for predicting ovulation in this species.
D000818:Animals; D001681:Biological Assay; D002144:Callithrix / Q000097:blood / Q000502:physiology*; D002143:Callitrichinae / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D004966:Estrogens, Conjugated (USP) / Q000097:blood; D004970:Estrone / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood; D010060:Ovulation; D010061:Ovulation Detection; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay
D004966:Estrogens, Conjugated (USP); D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; C017296:estrone sulfate