The preparation of interferon gamma-producing T-cell hybridomas from jacalin-stimulated T lymphocytes and the SH9 T-cell line.
Jacalin, a lectin(s) extracted from the seeds of Artocarpus integrifolia (Jackfruit), was shown to induce the production of gamma interferon (IFN gamma) in human peripheral blood mononuclear cells (PBMC) and human T-lymphocyte cultures. The amount of IFN gamma produced was enhanced in the presence of mezerein, a phorbol ester derivative. Fusion of jacalin stimulated T lymphocytes with the SH9 T-cell line resulted in the formation of T-cell hybridomas which spontaneously secreted IFN gamma. The spontaneous production of IFN gamma by T-cell hybridomas was not influenced by the presence of jacalin, although significant enhancement of production was observed when the cells were cultured in the presence of mezerein.
D000818:Animals; D002460:Cell Line; D017977:Deltaretrovirus; D004224:Diterpenes; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis*; D037102:Lectins / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008213:Lymphocyte Activation; D037121:Plant Lectins; D012192:Retroviridae Infections / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology
D004224:Diterpenes; D037102:Lectins; D037121:Plant Lectins; D013729:Terpenes; C043696:jacalin; C011309:mezerein; D007371:Interferon-gamma