title: Genome analysis of BK (WW) viral DNA cloned directly from human urine.
abstract: DNA from a third isolate of the WW strain of BK virus was prepared from human urine and was cloned into the BamHI site of pBR322. Restriction mapping showed a number of changes relative to the prototype BK viral DNA, including the presence of a new PstI, an SstI, and an AvaII site and the loss of a HindIII, an SstI, and an NcoI site. There is evidence for more extensive changes in the vicinity of the origin of replication.
mesh_terms: D001739:BK Virus / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D011120:Polyomavirus / Q000235:genetics*; D014556:Urine / Q000382:microbiology
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes