DNA replication of simian virus 40 chromatin in digitonin-treated and saponin-treated permeable cells.
Permeable cell systems have been developed by treatment of simian virus 40 (SV40)-infected cells with digitonin or saponin for studying in vitro DNA replication of chromatin. SV40 DNA replicated efficiently in these systems in the presence of the four deoxyribonucleoside triphosphates, ATP, Mg2+, and the proper ionic environment. Analysis of the time course of the replicated SV40 DNA I, II and replicating intermediates by gel electrophoresis and autoradiography indicated that SV40 DNA replicated completely in the form of nucleoprotein complexes (SV40 chromatin).
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D002843:Chromatin / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D004072:Digitonin / Q000494:pharmacology; D009698:Nucleoproteins / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology; D013539:Simian virus 40 / Q000378:metabolism*
D002843:Chromatin; D004279:DNA, Viral; D009698:Nucleoproteins; D012503:Saponins; D004072:Digitonin