Rapid detection of canine parvovirus in feces using monoclonal antibodies and enzyme-linked immunosorbent assay.
Monoclonal antibodies were used to develop a double antibody enzyme-linked immunosorbent assay for the detection of canine parvovirus (CPV) antigen in fecal samples. The assay was specific for the hemagglutinating protein of CPV and detected as little as 1.5 ng of virus within a 15-minute incubation period. The use of monoclonal antibodies against 2 epitopes on the CPV antigen permitted the simultaneous addition of test sample and enzyme-conjugated antibody, thus considerably simplifying the manipulations required for the assay. Results were visually determined without special instrumentation. Clinical studies revealed greater than 95% correlation between enzyme-linked immunosorbent assay results and hemagglutination titers.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000276:immunology; D006386:Hemagglutination Tests / Q000662:veterinary; D006389:Hemagglutinins, Viral / Q000032:analysis*; D006825:Hybridomas; D051379:Mice; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral