title: Rabbit liver microsomal metabolism of enclomiphene.
abstract: Metabolism of the triarylethylene antiestrogen enclomiphene (EC) has been studied in rabbit liver. Incubation of EC with microsomes in the presence of NADPH gave two metabolites, EC-1 and EC-2, as determined by thin-layer chromatography (TLC). EC-1 had mass-spectral features that were identical to those of synthetic desethylenclomiphene, and was indistinguishable from this compound by TLC and gas-liquid chromatography (GLC). EC-2 was similarly identical to synthetic 4-hydroxyclomiphene, but was separable from 3-hydroxyclomiphene by TLC ang GLC. EC-2 methyl ether was homogeneous with 4-methoxyclomiphene, but not with 4'-methoxyclomiphene, in GLC. These results suggest that enclomiphene is metabolized in a manner qualitatively similar to that reported for other structurally related antiestrogens.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D002996:Clomiphene / Q000031:analogs & derivatives* / Q000378:metabolism; D064695:Enclomiphene; D007536:Isomerism; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D011817:Rabbits; D013329:Structure-Activity Relationship
chemical_list: D002996:Clomiphene; D064695:Enclomiphene