[Interaction of n-alkanes with respiration and oxidative phosphorylation in rabbit heart mitochondria: n-nonane].
Studies "in vitro" on the effect of n-nonane on coupled rabbit heart mitochondria with both succinate and glutamate as substrates show that the hydrocarbon examined makes the membrane permeable to protons (uncoupling), to some matrix enzymes and to exogenous NADH. The effect increases with increasing n-alkane concentration (from 0 to 160 microgram/mg mitochondrial protein) and temperature (from 15 degrees to 38 degrees C). Furthermore at higher concentrations and temperatures NADH oxidase inhibition is observed, whereas on succinate oxidase a biphasic effect (activation at lower concentrations and inhibition at higher concentrations) is produced. However the results, qualitatively similar to those observed with n-hexane, exhibit features probably due to a longer chain and that can be ascribed to perturbations of the physical state of membrane lipids.
D000473:Alkanes / Q000494:pharmacology*; D000818:Animals; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D011817:Rabbits; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013696:Temperature
D000473:Alkanes; D005971:Glutamates; D009097:Multienzyme Complexes; D013386:Succinates; D009243:NAD; D018698:Glutamic Acid; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; C017573:nonane
Interazioni di n-alcani con la respirazione e fosforilazione ossidativa di mitocondri cardiaci di coniglio: n-nonano.