The effect of somatostatin (SRIF) on the release of amino acids from skeletal muscle.
1. Somatostatin (SRIF, somatotropin release inhibiting factor), at a concentration of 2 x 10(-8) M (32 ng/ml) decreased the rat of alanine release (approximately 45%) and increased glutamine release (approximately 30%) in in vitro preprations of m. extensor digitorum longus (EDL) muscle from 35--40 day old Wistar rats. These effects of SRIF were observed under both aerobic and anaerobic conditions. 2. SRIF increased the formation of 14CO2 from alanine but not from glutamine, glutamate, leucine, isoleucine or valine. 3. The incorporation of alanine, glutamine, glutamate, leucine, isoleucine and valine into muscle protein was unaffected by the presence of SRIF.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology*
D005971:Glutamates; D005973:Glutamine; D013004:Somatostatin; D003994:Bucladesine; D009584:Nitrogen; D000409:Alanine; D010100:Oxygen