A simple perfusion chamber for studying neurotransmitter release from cells maintained in monolayer culture.
A perfusion chamber is described for studying the efflux of putative neurotransmitters from CNS cells maintained in monolayer culture. We have used this apparatus to investigate the efflux of newly accumulated [3H]GABA from cell cultures of the early postnatal rat cerebellum.
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid