Phenotype characteristics of human B cells studied by Epstein-Barr virus infection. III. In vitro modulation of C3 receptor expression on human lymphoid cells.
An addition of dimethyl sulfoxide (DMSO) in culture medium enhanced the expression of receptor (R) for EACh (C3b-R) of the lymphoblastoid cells derived from immature B cells which were expressing little or no EACh-R. EACm-R (C3bi- and C3d-R) on the lymphoblastoid cells of bone marrow cells decreased remarkably (25 to 5%) during short term cultivation, though other markers remained unchanged. In addition, C3-R expression of the lymphoblastoid cell lines from bone marrow cells, which were established by EBV infection before and after 4-day cultivation, was quite similar to that of the cell of origin. These results, in accordance with our previous paper (Kudo, T. and Tachibana, T. (1984) Immunol. Lett. 8,343-348), support the idea that the sequential switching of C3 receptors, EACm-R----EACh-R, occurs during B cell differentiation.
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004854:Herpesvirus 4, Human; D006801:Humans; D016177:Macrophage-1 Antigen; D010641:Phenotype; D011951:Receptors, Complement / Q000187:drug effects
D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D004121:Dimethyl Sulfoxide