title: Investigations in the field of recombinant DNA technology performed in the "Stefan S. Nicolau" Institute of Virology.
abstract: A brief review is provided of the investigations in the field of recombinant DNA technology started in 1979 in the Central Laboratory for Nucleic Acids within the "Stefan S. Nicolau" Institute of Virology. The research efforts have been focused on the following main objectives: optimization of vector extraction, isolation and purification of restriction enzymes and of DNA ligase T4, transformation and transfection experiments, construction of recombinant DNA.
mesh_terms: D000047:Academies and Institutes / Q000266:history*; D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents; D011088:DNA Ligases / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000266:history*; D004317:Doxorubicin / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D005822:Genetic Vectors; D012383:Romania; D014162:Transfection / Q000187:drug effects; D014170:Transformation, Genetic
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004274:DNA, Recombinant; C010806:violamycins; D004317:Doxorubicin; D004262:DNA Restriction Enzymes; D011088:DNA Ligases; D004996:Ethidium