Expression of the c-fos gene and of an fos-related gene is stimulated by platelet-derived growth factor.
Complementary DNA clones of genes induced by platelet-derived growth factor (PDGF) in BALB/c-3T3 cells were isolated; one such clone contains a domain having nucleotide sequence homology with the third exon of c-fos. This nucleotide sequence homology is reflected in the predicted amino acid sequences of the gene products. Under low stringency conditions, the mouse v-fos gene cross-hybridizes with the PDGF-inducible complementary DNA clone. However, the messenger RNA transcripts of mouse c-fos and the new fos-related gene can be distinguished by gel electrophoresis and by S1 nuclease analysis. Expression of the authentic c-fos gene is induced by PDGF and superinduced by the combination of PDGF and cycloheximide.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004720:Endonucleases; D005796:Genes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D009857:Oncogenes / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic / Q000187:drug effects
D004251:DNA Transposable Elements; D010982:Platelet-Derived Growth Factor; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases