[Demonstration of neuronal activities in primary cultures from fetal mouse hypothalamus maintained in a serum-free medium].
Fetal neuronal cells dissociated from mouse hypothalamus are able to grow in a serum-free medium for two to four weeks. Several neuronal activities have been measured during the in vitro cell development. Whereas glutamic acid decarboxylase (EC 4.1.1.15) activity disappears, those of thyroliberin (TRH) and tyrosine hydroxylase (EC. 1.14.16.2) remain stable, and acetylcholine transferase one (EC 2.3.1.6) rises earlier than in a serum supplemented medium. These results show that primary cultures grown in serum-free medium offer a promising model for studying neuronal activity differentiation.
D000818:Animals; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003470:Culture Media; D005333:Fetus / Q000166:cytology; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D003470:Culture Media; D013973:Thyrotropin-Releasing Hormone; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase
Mise en évidence d'activités neuronales dans des cultures primaires d'hypothalamus de Souris foetale maintenues en milieu sans sérum.