title: [GTP--a substrate for 3',5'-AMP-dependent phosphorylation of synaptic membrane proteins].
abstract: Using the method of electrophoresis in polyacrylamide gel (PAGE) it is shown that not only ATP, but GTP may play the role of phosphate donor for 3':5'-monophosphate (cAMP)-dependent phosphorylation. GTP is incorporated predominantly in the same "classic" 3':5'AMP-dependent phosphorylated proteins (with Mr 86 000, 82 000, 68 000, 61 000, 54 000) as that from ATP does. EGTA treatment of synaptic membranes enhances 3':5'-monophosphate (cAMP)-dependent phosphorylation with GTP used as a phosphate donor.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013570:Synaptic Membranes / Q000201:enzymology / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D004533:Egtazic Acid; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases
vernacular_title: GTP--substrat dlia 3':5'-AMP-zavisimogo fosforilirovaniia belkov sinapticheskikh membran.