The T-DNA of Agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants.
Plants regenerated from tobacco hairy root callus cultures were fertilized by self-pollination, and healthy, morphologically normal R1 offspring were obtained. Three of these R1 seedlings were analyzed for the presence of T-DNA and synthesis of the T-DNA-specific compound agropine. All three R1 plants analyzed contained the same full-length T-DNA as the parental regenerant, while only two showed agropine synthesis.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D008540:Meiosis; D009693:Nucleic Acid Hybridization; D059828:Plant Cells; D010944:Plants / Q000235:genetics*; D012038:Regeneration; D012231:Rhizobium / Q000166:cytology / Q000235:genetics*
D004269:DNA, Bacterial; C036483:T-DNA; D004262:DNA Restriction Enzymes