GRF (somatocrinin) stimulates release of neurotensin, calcitonin and cAMP by a rat C cell line.
We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5-180 min in serum-free defined medium. CT, NT and cAMP are measured by RIA. At a maximally effective concentration of GRF (.1-1.0 nM), there is a 2-3 fold stimulation of CT release at 60-90 min with peak release at 180 min. In contrast, GRF causes a rapid 4-6 fold increase of NT release within 5-15 min. In 44-2 C cells there is a 4-40 fold stimulation of cAMP release by GRF. We conclude that in 44-2 C cells GRF stimulates release of NT and cAMP and show for the first time the effect of this peptide on release of CT.
D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D009496:Neurotensin / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D013964:Thyroid Neoplasms / Q000378:metabolism*; D013997:Time Factors
D010446:Peptide Fragments; D009496:Neurotensin; D002116:Calcitonin; D013007:Growth Hormone-Releasing Hormone; D000242:Cyclic AMP