An examination of the relative resistances to aflatoxin B1 and susceptibilities to gamma-glutamyl p-phenylene diamine mustard of gamma-glutamyl transferase negative and positive cell lines.
Two epithelial cell lines have been derived from rat liver. One, containing only low levels of gamma-glutamyl transferase (GGT) was obtained from normal liver and the other, containing high levels of the enzyme was isolated from an aflatoxin B1-induced hepatoma. The GGT levels present in the two cell lines have been examined by histochemical staining, by a fluorescence assay using disrupted cells, and also by intact fluorescence-labelled cells in a laser flow cytofluorimeter. The cells containing high levels of GGT have been found to be less sensitive to microsomally-activated aflatoxin B1 than are the GGT negative cells, a feature of the in vivo situation. Evidence for activation of the gamma-glutamyl derivative of an alkylating mustard analogue of p-phenylene diamine by the GGT positive cells is presented. This finding could be of relevance to the possible chemotherapy of GGT-rich lesions in vivo.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology*; D000478:Alkylation; D000816:Aniline Mustard / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002273:Carcinogens; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000348:Aflatoxins; D002273:Carcinogens; D009588:Nitrogen Mustard Compounds; C032019:gamma-glutamyl 4-phenylene diamine mustard; D016604:Aflatoxin B1; D000816:Aniline Mustard; D005723:gamma-Glutamyltransferase