Purification and protein composition of endogenous rat viruses.
Endogenous retroviruses are not in the majority of cases the cause of any neoplasia, except for the laboratory conditions. As far as they might serve for the evolution of pathogenic retroviruses more attention should have been paid to them. In this paper we introduce some approaches to the purification of rat endogenous retroviruses to such a degree of purity that enabled satisfactory SDS-PAGE analysis of its structural proteins. Purities of samples obtained by usual purification methods, long-term isopycnic centrifugation at a high gravity force and velocity centrifugation are compared. Protein profile of rat endogenous virus in SDS-PAGE is compared with the ones of other retroviruses. For the first time the evidence was obtained for the striking similarity between electrophoretic protein profile of rat endogenous virus WERC and feline leukemia virus. The major structural proteins of rat endogenous retrovirus and feline leukemia virus cannot be distinguished even when resolution long gradient PAGE had been employed. The accordance of electrophoretic mobilities of major structural proteins in SDS-PAGE can indicate the relatedness of retroviruses.
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000302:isolation & purification; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D005256:Leukemia Virus, Feline / Q000302:isolation & purification; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012190:Retroviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins