An in-vitro system for determining the activity of compounds against the intracellular amastigote form of Leishmania donovani.
A method has been defined for infecting primary mouse peritoneal macrophages with amastigotes of Leishmania donovani in vitro, and analysing the response of the infected macrophages to treatment with drugs. The growth of intracellular amastigotes was inhibited by all clinically used antileishmanial drugs. Toxic effects on macrophages were observed with some drugs. Other experimental antileishmanial compounds were active in this system. This test is proposed as an initial screening test for the discovery of novel antileishmanial compounds.
D000818:Animals; D000967:Antimony Sodium Gluconate / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology* / Q000633:toxicity; D003470:Culture Media; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D007891:Leishmania / Q000187:drug effects*; D007898:Leishmaniasis, Visceral / Q000469:parasitology; D008264:Macrophages / Q000187:drug effects / Q000469:parasitology; D008297:Male; D051379:Mice
D000981:Antiprotozoal Agents; D003470:Culture Media; D005839:Gentamicins; D000967:Antimony Sodium Gluconate