Quantitative recovery of alkaline phosphatase and gamma-glutamyl transferase isoenzymes after polyacrylamide gel electrophoresis.
Loss of activity of the isoenzymes of gamma-glutamyl transferase and alkaline phosphatase has been shown to occur during electrophoresis on polyacrylamide gel. After studying the possible factors concerned in this loss, reasonable recovery from the gel can be obtained only for the isoenzyme staying at the origin. Maximum recovery is 60% for origin gamma-glutamyl transferase and 92% for origin alkaline phosphatase.
D000469:Alkaline Phosphatase / Q000097:blood*; D001646:Bile / Q000201:enzymology; D001842:Bone and Bones / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D015032:Zinc / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000097:blood*
D007527:Isoenzymes; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D015032:Zinc