[Urinary elimination of multiple forms of gamma-glutamyltranspeptidase and aminopeptidase (author's transl)].
Concentrated and dialyzed 24-h urines of healthy persons were separated by 105000 X g ultracentrifugation into a pellet (P105) and a supernatant (S105) fraction. Chromatography of the P105 fraction on Sepharose 4B and 2B revealed that gamma-glutamyltranspeptidase and aminopeptidase activities had a molecular mass of 20 X 10(5) to 40 X 10(6), whereas in the S105 fraction soluble gamma-glutamyltranspeptidase and aminopeptidase had 86000 and 160000, respectively. Triton X-100 solubilization was performed on the P105 fraction and on a human renal cortex 40000 X g pellet, used as a reference. All the activity was recovered in both cases in a single peak of detergent gamma-glutamyltranspeptidase and detergent aminopeptidase eluted by filtration on Ultrogel Ac A22. Apparent molecular mass of Triton X-100 solubilized urinary and renal enzymes were 250000 and 243000 for gamma-glutamyltranspeptidase, and 298000 for both aminopeptidases. Protease solubilized forms were obtained by trypsic digestion of detergent urinary and renal forms. Both gamma-glutamyltranspeptidases were found to have an apparent molecular mass of 86000 on Sephadex G 150, which is identical to the value found for the S105 urinary gamma-glutamyltranspeptidase. The aminopeptidases had 238000 and 232000, which is a higher value than the molecular mass of the S105 urinary aminopeptidase. This letter could be a degraded form of the renal aminopeptidase. These findings suggest that gamma-glutamyltranspeptidase and aminopeptidase in the P105 fraction are similar to native renal enzymes. Evaluation of the P105 fraction enzymatic activities may thus be useful in the diagnosis of tubular damage.
D000626:Aminopeptidases / Q000652:urine*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D007672:Kidney Cortex / Q000201:enzymology; D008297:Male; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility; D014357:Trypsin / Q000378:metabolism; D014461:Ultracentrifugation; D005723:gamma-Glutamyltransferase / Q000652:urine*
D007527:Isoenzymes; D011092:Polyethylene Glycols; D017830:Octoxynol; D005723:gamma-Glutamyltransferase; D000626:Aminopeptidases; D014357:Trypsin
Etude des formes d'élimination urinaire de la gamma-glutamyltranspeptidase et de l'aminopeptidase.