Early appearance of histochemically altered hepatocyte foci and liver tumors in female rats treated with carcinogens one day after birth.
A single i.p. injection of diethylnitrosamine (DEN) or benzo[a]pyrene (BAP) in 1-day-old female rats produced a high incidence of gamma-glutamyltranspeptidase-(GGT)-positive hepatocyte foci within 4 weeks after the rats were weaned onto a 0.05% phenobarbital diet; the injection of benzo[e]pyrene did not produce foci under these conditions. Liver tumors appeared in rats treated with DEN within 8 weeks after weaning and in BAP-treated rats within 16 weeks after weaning. The results suggest that the treatment protocol used in this study may increase the utility of the liver tumorigenesis model as a broadly applicable in vitro system for the rapid detection of tumorigenic potential in environmental contaminants.
D000818:Animals; D000831:Animals, Newborn; D001580:Benzopyrenes / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D004052:Diethylnitrosamine / Q000494:pharmacology; D005260:Female; D006651:Histocytochemistry; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D001580:Benzopyrenes; D002273:Carcinogens; D004052:Diethylnitrosamine; D005723:gamma-Glutamyltransferase