Histamine inhibition of the in vitro induction of cytotoxic T-cell responses.
Addition of 10-3 to 10-6 M histamine (H)3 to mixed leukocyte cultures (MLCs) inhibited primary in vitro induction of cytotoxic T lymphocytes (CTLs) specific for either allogeneic or trinitrophenol-modified syngeneic target cells. The use of specific H agonists implicated H2 but not H1 receptor triggering in the mediation of these effects. Unlike in vivo-induced allogenic CTLs, the addition of H to assay culture failed to influence the effector function of in vitro-induced CTLs of either specificity. Kinetic studies showed that this difference might be due to loss of functional H receptors after the initiation of the in vitro MCL, and demonstrated that H interferes with an early event in the generation of CTLs. These data indicate that H receptors are not merely markers for CTL precursors, but that they are functional receptors, and suggest that H may play an important role in regulating both the generation and effector function of CTLs in vivo.
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D017259:Dimaprit; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects*; D013890:Thiourea / Q000494:pharmacology
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D006632:Histamine; D013890:Thiourea; D017259:Dimaprit