RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.
A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence: (sequence; see test) By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.
D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012242:Rhodobacter sphaeroides / Q000201:enzymology*; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; C043032:endodeoxyribonuclease RsrII; D015252:Deoxyribonucleases, Type II Site-Specific