Identification of a glutathione conjugate of cambendazole formed in the presence of liver microsomes.
The incubation of multiply labeled (2H, 3H, 13C, 14C) cambendazole and glutathione with hepatic microsomes from phenobarbital-dosed hamsters results in the formation of polar metabolites. The major metabolite has been characterized by a variety of isotopic, spectrometric, chromatographic, and degradative/synthetic techniques as a glutathione conjugate of cambendazole in which substitution is on the 4-position of the benzimidazole nucleus. The same metabolite is produced by hepatic microsomes from the rat.
D000818:Animals; D001345:Autoradiography; D001562:Benzimidazoles / Q000378:metabolism*; D002159:Cambendazole / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D005978:Glutathione / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D001562:Benzimidazoles; D002159:Cambendazole; D005978:Glutathione