The stability and potency of vaccines prepared from inactivated foot-and-mouth disease virus concentrates.
The stability of 146S particles in concentrates of foot-and-mouth disease virus stored at 4 degrees C was similar to that of 146S particles in a conventional virus preparation. Proteolytic degradation of VPl was not observed in the stored conventional virus preparation or inhibitor-supplemented concentrate but was observed in a supplement-free concentrate. The potencies of vaccines made from the conventional and concentrated preparations and stored in parallel at 4 degrees C appeared to decrease after 16 weeks. The vaccines made from the supplement-free concentrate and the Trasylol supplemented concentrate appeared to be at least as potent as the conventional vaccine and were clearly superior to vaccine made from ox serum supplemented concentrate.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D005537:Aphthovirus / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D004355:Drug Stability; D004356:Drug Storage; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005615:Freezing; D006168:Guinea Pigs; D009500:Neutralization Tests; D009584:Nitrogen; D013997:Time Factors; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology* / Q000592:standards
D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D009584:Nitrogen