Freeing rabies virus of the neuroallergenic factor from brain tissue.
A method of fixed rabies virus purification from infected sheep brains was proposed. It consisted of suspending the brain tissue in phosphate-buffered hypertonic (0.3 M) NaCl solution, shaking at 37 degrees C and low speed centrifugation at the same temperature. From 65 to 85% ballast proteins were removed, the neuroallergenic activity of the material was lost, but practically no losses of the virus occurred.
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D001921:Brain / Q000382:microbiology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology; D006168:Guinea Pigs; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D011819:Rabies Vaccines / Q000009:adverse effects / Q000276:immunology / Q000302:isolation & purification*; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*; D012756:Sheep
D000485:Allergens; D009419:Nerve Tissue Proteins; D011819:Rabies Vaccines