[Synthesis of a full-length DNA copy of the hemagglutinin gene of the the influenza virus A H1N1 subtype, its cloning and primary structure].
Full-length DNA-copy of hemagglutinin gene RNA of influenza A virus strain A/Leningrad/54/1 was synthesized and cloned in E. coli plasmid pBR 327. Its primary structure was determined by a modified Maxam - Gilbert procedure. The nucleotide sequence of this gene was compared with sequences of analogous genes of influenza strains A/USSR/90/77 and A/PR/8/34. An addition of one nucleotide in non-translated region was found in the former.
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D005814:Genes, Viral; D006388:Hemagglutinins / Q000235:genetics*; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D013698:Templates, Genetic
D004279:DNA, Viral; D006388:Hemagglutinins; D004247:DNA; D004262:DNA Restriction Enzymes
Sintez polnorazmernoĭ DNK-kopii gena gemaggliutinina virusa grippa A H1N1-podtipa, ee klonirovanie i opredelenie pervichnoĭ struktury.