Agonist potency determination by patch clamp analysis of single glutamate receptors.
Agonists (L-quisqualate, L-glutamate and L-cysteine sulphinate) of the locust muscle glutamate receptor differ in potency. Patch clamp analysis of single glutamate receptors reveals that the relative potencies of agonists are determined both by the life-times of the channels that they gate and their probability of activating the glutamate receptor-ionophore complex. Furthermore, agonists which most readily activate the receptor channel complex gate channels with longer life-times than agonists which are less efficient in this respect.
D000818:Animals; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004558:Electric Stimulation; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006110:Grasshoppers; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D009431:Neural Conduction / Q000187:drug effects*; D009469:Neuromuscular Junction / Q000187:drug effects*; D018377:Neurotransmitter Agents; D010069:Oxadiazoles / Q000494:pharmacology; D016318:Quisqualic Acid; D011956:Receptors, Cell Surface / Q000187:drug effects*; D017470:Receptors, Glutamate; D013268:Stimulation, Chemical
D005971:Glutamates; D007473:Ion Channels; D018377:Neurotransmitter Agents; D010069:Oxadiazoles; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D016318:Quisqualic Acid; D003545:Cysteine; C013461:cysteine sulfinic acid