Purification of Newcastle disease virus by chromatography on controlled-pore glass bead column.
Newcastle disease virus (NDV) purified by sucrose density gradient centrifugation was chromatographed on a controlled-pore glass (CPG) bead column. By this procedure, the contaminating ribonuclease activity was reduced by 94-96% and the specific viral haemagglutinating activity increased from 3-to 5-fold. Purified NDV moved in two fractions in free electrophoresis.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002845:Chromatography / Q000379:methods*; D046508:Culture Techniques; D006389:Hemagglutinins, Viral / Q000032:analysis; D009522:Newcastle disease virus / Q000032:analysis / Q000302:isolation & purification*; D012260:Ribonucleases / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis
D006389:Hemagglutinins, Viral; D014764:Viral Proteins; D012260:Ribonucleases