title: Chromatographic elution profiles of angiotensin converting enzyme measured by the methods using different substrates in the plasma from normotensive and hypertensive subjects.
abstract: The angiotensin converting enzyme (ACE) activity in the plasma collected from normotensive and hypertensive patients was measured chromatographically by two different methods using hippuryl-histidyl-leucine (HHL-ACE) and synthetic angiotensin I (AI-ACE) as substrates. A single peak was observed in the elution profiles of HHL-ACE on both Sephadex G 150 column and DEAE Sephadex A 50 cellulose column chromatographies. A single peak of AI-ACE appeared on the gel-filtration. However, several peaks of AI-ACE were seen on DEAE Sephadex A 50 cellulose column chromatography, suggesting that there are various types of ACE which hydrolyze angiotensin I but not HHL.
mesh_terms: D000328:Adult; D000803:Angiotensin I / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology*; D007527:Isoenzymes / Q000097:blood; D008297:Male; D009842:Oligopeptides / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D012083:Renin / Q000097:blood
chemical_list: D007527:Isoenzymes; D009842:Oligopeptides; C010980:hippuryl-histidyl-leucine; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D012083:Renin