Channel currents during spontaneous action potentials in embryonic chick heart cells. The action potential patch clamp.
Single-channel currents were recorded with the cell-attached patch-clamp technique from small clusters (2-20 cells) of spontaneously beating 7-d embryo ventricle cells. Because the preparation was rhythmically active, the trans-patch potential varied with the action potential (AP). The total current through the patch membrane was the patch action current (AC). ACs and APs could be recorded simultaneously, with two electrodes, or sequentially with one electrode. Channel activity, which varied depending on the number and type of channels in the patch, was present during normal cell firing. This method can reveal the kinetics and magnitudes of the specific currents that contributed to the AP, under conditions that reflect not only the time and voltage dependence of the channels, but also environmental factors that may influence channel behavior during the AP.
D000200:Action Potentials; D000818:Animals; D002642:Chick Embryo; D004553:Electric Conductivity; D004594:Electrophysiology / Q000379:methods; D006321:Heart / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D016276:Ventricular Function
D007473:Ion Channels