[Evidence that electrophoretic-mobility (EM) test is of no value for the diagnosis of cancer in children (author's transl)].
After incubation with encephalitogenic factor (EF) the capacity of lymphocytes to inhibit the migration of tanned, stabilized sheep-erythrocytes in the electric field was tested in 133 children (52 tumor- and 81 control patients). Tumor patients (on and off treatment) as well as children suffering from non-inflammatory diseases showed a significant migration inhibition as compared with healthy controls (p less than 0.05). However, in only 3/23 patients on, and 3/35 off therapy, an unequivocal inhibition of more than 8% could be demonstrated. The repeated examination of 8 individual patients yielded an unsatisfactory reproducibility; results were independent of the EF concentration ranging between 200-600 microgram EF/ml. We think that the EM-Test provides no useful information for the detection of malignant disease in children.
D000293:Adolescent; D000818:Animals; D002464:Cell Migration Inhibition; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D008214:Lymphocytes; D009369:Neoplasms / Q000175:diagnosis*; D012756:Sheep; D013997:Time Factors
Unbrauchbarkeit des Elektrophorese-Mobilitäts-(EM-)tests zur Tumordiagnostik bei Kindern.