Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.
A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization
D004274:DNA, Recombinant; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D004262:DNA Restriction Enzymes