Fluidity of human erythrocyte membrane and effect of chlorpromazine on fluidity and phase separation of membrane.
The fluidity of human erythrocyte membrane, and the effect of chlorpromazine at prelytic and lytic concentrations on the fluidity have been studied by using three kinds of fatty acid spin labels and measuring the temperature dependence of Mg2+-ATPase activity. The Arrhenius plot of the apparent rotational correlation time, tau c, for probes I(12,3) and I(5,10) showed an abrupt discontinuity at about 30 degrees C, and the plot for I(1,14) at 25 degrees C, indicating that a large difference in the fluidity exists between the interior and the outer surface of the lipid bilayer. The portions of the fatty acid chain near the ten carbon bond lengths removed from the bilayer surface became more fluid by chlorpromazine treatment; there was a decrease in the break point to around 26 degrees C following treatment with 0.6 or 1 mM of the drug. Two breaks at 21 and 30 degrees C in the Arrhenius plot of the Mg2+-ATPase activity were observed in normal erythrocyte membrane. The activation energy of the Mg2+-ATPase reaction has the values of 3.0 and 22.1 kcal/mol above the upper break and below the lower break, respectively. The drug exposure induced only a slight shift in the break temperatures, while the treatment significantly enhanced the associated activation energies of the reaction. These results suggest that the boundary phospholipids of the Mg2+-ATPase in the membrane are probably more rigid than the bulk lipids.
D000251:Adenosine Triphosphatases / Q000097:blood; D017301:Ca(2+) Mg(2+)-ATPase; D002746:Chlorpromazine / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000097:blood; D013113:Spin Labels; D013696:Temperature; D013816:Thermodynamics
D008051:Lipid Bilayers; D008563:Membrane Lipids; D013113:Spin Labels; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D002746:Chlorpromazine