Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro.
A new assay for 1,25-dihydroxyvitamin D3 in biological fluids is described, in which measurement is based on the translocation of the hormone across cell membranes and its binding to specific cytoplasmic receptors inside target cells in culture. The technique obviates the need for chromatographic separation of 1,25(OH)2D3 from other metabolites of vitamin D3.
D000818:Animals; D002117:Calcitriol; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000378:metabolism*; D006887:Hydroxycholecalciferols / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*; D011874:Radiometry / Q000379:methods*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D011956:Receptors, Cell Surface; D002117:Calcitriol