title: Eosinophil and neutrophil chemotactic activities of adult worm extracts of Schistosoma japonicum in vivo and in vitro.
abstract: Large numbers of eosinophils and neutrophils attracted to the soluble extract of Schistosoma japonicum adult worms (SjAW-ext) were detected at the injection site of normal guinea pig skin. Eosinophil and neutrophil chemotactic activities were also confirmed in in vitro assay by using blind-well chambers with Millipore filters in dose-dependent fashion. Two components of SjAW-ext showed eosinophil chemotactic activity; one was in the high molecular weight fraction (JAE-H), estimated to be more than 440,000 daltons, the other in the low molecular weight fraction (JAE-L) obtained by Sephadex G-200 gel filtration. High neutrophil chemotactic activity was detected in the JAE-L. These eosinophil and neutrophil chemotactic activities were also detected in culture fluid of S. japonicum adult worms. Eosinophil chemotactic factor (ECF) of JAE-H was stable to heating (100 C, 30 min) and pronase digestion, but completely destroyed by periodate oxidation. It is suggested that the ECF of JAE-H is a glycoprotein. JAE-L was also stable to heating (56 and 100 C, 30 min) and pronase digestion for eosinophil chemotaxis. Possible roles of those activities in schistosome infections are discussed.
mesh_terms: D000818:Animals; D001681:Biological Assay; D002630:Chemotactic Factors / Q000032:analysis*; D002631:Chemotactic Factors, Eosinophil / Q000032:analysis*; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D005260:Female; D006168:Guinea Pigs; D006358:Hot Temperature; D016209:Interleukin-8; D008297:Male; D008970:Molecular Weight; D009439:Neuraminidase; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011402:Pronase; D012549:Schistosoma japonicum / Q000032:analysis*
chemical_list: D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D016209:Interleukin-8; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase; D011402:Pronase