[Immunohistochemical findings in intrathoracic tumors. V. Isoenzymes of nonspecific esterases in lung tissue and bronchial cancers].
With histopolyacrylamide electrophoresis (HPAGE) no marked differences in isozyme pattern of non-specific esterase between lung tissue and bronchial carcinomas were found. Only 3 of 19 bronchial carcinomas with histochemically detectable activity of non-specific esterase in tumor tissue showed an additional isozyme with fast mobility. Acetone acts in histochemical reactions as an activator or inhibitor of activity of non-specific esterase. Macrophages, epitheloid cells, and giant cells are inhibited by acetone. In contrast, bronchial epithelial cells are activated by acetone. Bronchial carcinomas showed a different behaviour. Non-specific esterase activity is activated in epidermoid carcinomas but not in adenocarcinomas. This shows close connections to the development of epidermoid carcinomas from bronchial epithelium. Additionally in non-fixed cryostat sections of bronchial epithelium with the aid of non-specific esterase groups of strong reacting cells were demonstrated, which are probably Kultschitzky cells. Acetone inhibits completely activity of esterase in these cells.
D000230:Adenocarcinoma / Q000201:enzymology; D002283:Carcinoma, Bronchogenic / Q000201:enzymology*; D018288:Carcinoma, Small Cell / Q000201:enzymology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000378:metabolism*; D006652:Histological Techniques; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008168:Lung / Q000201:enzymology*; D008175:Lung Neoplasms / Q000201:enzymology*
D007527:Isoenzymes; D004950:Esterases
Immunhistochemische Befunde bei intrathorakalen Tumoren. V. Isoenzyme der unspezifischen Esterase im Lungengewebe und in Bronchialkarzinomen.