[Interaction of prazosin with cardiac membranes. Method of studying the peripheral action of alpha-adrenergic agents].
3H-prazosin binds to rat cardiac membranes in a rapid (steady state obtained before 30 minutes) and reversible (t 1/2 = 15 min) manner. The binding of this ligand is a saturable process with high affinity (KD 25 degrees C = 0,20 nM) and a maximum number of sites of 75.7 +/- 11.2 fmoles/mg of protein. Specificity studies indicate that this ligand binds to sites possessing the characteristics of alpha-1-adrenoceptors.
D000317:Adrenergic alpha-Antagonists / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D006321:Heart / Q000187:drug effects*; D008566:Membranes / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism; D011224:Prazosin / Q000378:metabolism*; D011799:Quinazolines / Q000378:metabolism*; D051381:Rats
D000317:Adrenergic alpha-Antagonists; D011799:Quinazolines; D011224:Prazosin
Interaction du prazosin avec les membranes cardiaques. Méthode l'étude de l'action périphérique des agents alpha-adrénergiques.