Photoaffinity labeling of different benzodiazepine receptors at physiological temperature.
Irreversible labeling of benzodiazepine receptors in membranes from cerebellum or hippocampus was compared at 0 degrees C using [3H]flunitrazepam as a photoaffinity ligand. [3H]Flunitrazepam reproducibly and irreversibly labeled mainly one protein (P51) in cerebellum and at least two proteins (P51 and P55) in hippocampus at both temperatures. Differential inhibition at 37 degrees C of irreversible [3H]flunitrazepam binding to the individual proteins by several selective benzodiazepine receptor ligands supports the hypothesis that P51 and P55 are associated with different benzodiazepine receptors.
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002243:Carbolines / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D005445:Flunitrazepam / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D010777:Photochemistry; D011724:Pyridazines / Q000494:pharmacology; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D000345:Affinity Labels; D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D002243:Carbolines; D011724:Pyridazines; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D005445:Flunitrazepam; C021604:CL 218872; C024915:beta-carboline-3-carboxylic acid ethyl ester; C039103:2-oxoquazepam