Continuous spectrophotometric assay for aminoacyl-tRNA synthetases.
A simple, continuous assay for aminoacyl-tRNA synthetases utilizing a commercially available pyrophosphate assay reagent kit was demonstrated. The method coupled aminoacyl-tRNA synthetase activity with pyrophosphate-dependent fructose-6-phosphate kinase, aldolase, triosephosphate isomerase, and glycerophosphate dehydrogenase. PPi formation was correlated with the oxidation of NADH, and was monitored continuously by the decrease of absorbance at 340 nm.
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D001126:Arginine-tRNA Ligase / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D011756:Diphosphates / Q000032:analysis; D006801:Humans; D010920:Placenta / Q000201:enzymology; D011933:Reagent Kits, Diagnostic; D013053:Spectrophotometry / Q000379:methods
D011756:Diphosphates; D011933:Reagent Kits, Diagnostic; D000604:Amino Acyl-tRNA Synthetases; D001126:Arginine-tRNA Ligase