[Characteristics of the ionic composition and ultrastructure of Bordetella pertussis in controlled regulation of the mineral element content in a growth medium].
The content of trace elements necessary for the normal growth of bacteria was found to have no effect on the intracellular concentration of Ca2+ and Al3+. The content of Cu2+, Fe3+, Mn2+, Mg2+ was considerably reduced. The addition of Mg2+ at different concentrations to this culture medium stimulated the capacity of cells for accumulating not only Mg2+, but also some other ions. Their maximum intracellular concentration was observed when the concentration of Mg2+ in the culture medium was 41 mM. The growth of microbial cells in the standard culture medium containing Mg2+ at a concentration of 4 mM was accompanied by the increased consumption of elements actively participating in redox reactions (Cu2+, Fe3+, Mn2+). Shifts in the ionic composition of microbial cells were manifested by the morphological features of B. pertussis, linked with the increased synthesis of crystalloid structures. The influence of Mn2+, Al3+, Zn2+ at different concentrations on the ionic composition and morphology of B. pertussis was studied.
D001886:Bordetella pertussis / Q000378:metabolism* / Q000648:ultrastructure*; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007477:Ions; D008854:Microscopy, Electron; D014131:Trace Elements / Q000032:analysis / Q000378:metabolism*
D003470:Culture Media; D007477:Ions; D014131:Trace Elements
Osobennosti ionnogo sostava i ul'trastruktury Bordetella pertussis v usloviiakh napravlennoĭ reguliatsii soderzhaniia nekotorykh mineral'nykh élementov v srede vyrashchivaniia.