Termination of transcription in the mouse alpha-amylase gene Amy-2a occurs at multiple sites downstream of the polyadenylation site.
We have delimited the region of transcription termination in the alpha-amylase gene Amy-2a. Mapping of in vitro elongated nascent transcripts to Amy-2a restriction fragments indicates that transcription terminates in a region between 2.5 and 4 kb downstream of the polyadenylation site. These runoff transcription experiments, combined with S1 nuclease mapping of nuclear transcripts at steady state, suggest that transcription termination occurs at multiple sites.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005796:Genes; D051379:Mice; D008805:Mice, Inbred A; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000201:enzymology; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D004262:DNA Restriction Enzymes; D000516:alpha-Amylases