Isolation and chemical characterization of somatostatin-28 from rat hypothalamus.
A peptide with somatostatin-like immuno- and bioactivity has been isolated from an aqueous extract of 96 800 rat hypothalamic by means of immunoaffinity chromatography, gel filtration and reverse-phase high-performance liquid chromatography (HPLC). Chemical characterization by amino acid analysis, tryptic peptide mapping and retention behavior in two HPLC system showed that the peptide was indistinguishable from somatostatin-28 previously characterized from several species. This evidence suggests that rat hypothalamic somatostatin-28 is identical in structure to porcine and ovine somatostatin-28.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007031:Hypothalamus / Q000032:analysis*; D008970:Molecular Weight; D011498:Protein Precursors / Q000302:isolation & purification*; D051381:Rats; D013004:Somatostatin / Q000302:isolation & purification*; D053561:Somatostatin-28
D000596:Amino Acids; D011498:Protein Precursors; D013004:Somatostatin; D053561:Somatostatin-28