Immunoperoxidase-immunogold double labelling in immunoelectronmicroscopy of large granular lymphocytes.
A method for better characterization of mononuclear cell subpopulations using detection of 2 surface antigens simultaneously in electron microscopy was developed with immunoperoxidase-immunogold double labelling. Monoclonal antibodies of IgG and IgM classes were used in the first step, and colloidal gold-labelled anti-mouse IgG antibody and peroxidase-labelled anti-mouse IgM antibody (mu chain-specific) in the second step. Immunoelectronmicroscopy with such double labelling improves ultrastructural analysis of mononuclear cell subpopulations.
D000954:Antigens, Surface / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006048:Gold Colloid, Radioactive; D006801:Humans; D007124:Immunoenzyme Techniques; D007694:Killer Cells, Natural / Q000276:immunology / Q000648:ultrastructure*; D008875:Middle Aged
D000954:Antigens, Surface; D006048:Gold Colloid, Radioactive