title: Food dye, erythrosin B, inhibits ATP-dependent calcium ion transport by brain microsomes.
abstract: The widely-used food dye Erythrosin B inhibited ATP-dependent Ca2+ accumulation by rat brain microsomes, half-maximal inhibition requiring 1 microM dye. Addition of 0.5-20 microM dye to microsomes preloaded with Ca2+ did not cause any net Ca2+ release. 10 microM dye produced a constant inhibition of Ca2+ accumulation as the intravesicular free Ca2+ was lowered suggesting that, at low concentrations, it acts on the uptake system only. Ca2+ accumulation was ten-fold more sensitive to the dye than Erythrosin B-induced neurotransmitter release reported by others. Higher dye concentrations (100 microM) caused Ca2+ release.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects / Q000528:radiation effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D004923:Erythrosine / Q000494:pharmacology*; D005452:Fluoresceins / Q000494:pharmacology*; D005505:Food Coloring Agents / Q000494:pharmacology*; D008027:Light; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005452:Fluoresceins; D005505:Food Coloring Agents; D000252:Calcium-Transporting ATPases; D004923:Erythrosine; D002118:Calcium