title: [Effect of actinomycin D on the reproduction of the Machupo virus].
abstract: The effect of inhibitors of mRNA synthesis (actinomycin D and alpha-amanitin) and DNA replication (mitomycin C and ethidium bromide) on Machupo virus reproduction was studied. Actinomycin D (1.0-4.0 micrograms/ml) and alpha-amanitin (10.0 micrograms/ml) added immediately after adsorption reduced the infectious virus titre by 2 lg or more. Actinomycin D inhibited virus reproduction not only at the early but also at the later stages of infection. Mitomycin C and ethidium bromide did not inhibit Machupo virus reproduction. The cells treated with actinomycin D at early and late stages of infection were found to contain immunoprecipitable virus-specific proteins 78, 64, and 37 KD described previously. The total amount of virus-specific proteins in the inhibitor-treated cells was reduced 3.7 and 2,6-fold after addition of actinomycin at 0 and 12 h postinfection, respectively, as compared with untreated cells. The mechanism of action of actinomycin D on the Machupo virus reproduction is discussed.
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D001116:Arenaviridae / Q000187:drug effects*; D006477:Arenaviruses, New World / Q000187:drug effects* / Q000502:physiology; D003609:Dactinomycin / Q000494:pharmacology*; D004996:Ethidium / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000546:Amanitins; D008937:Mitomycins; D014764:Viral Proteins; D003609:Dactinomycin; D016685:Mitomycin; D004996:Ethidium
vernacular_title: Vliianie aktinomitsina D na reproduktsiiu virusa Machupo.