[High-performance liquid chromatography of peptide bioregulators, their fragments and derivatives. I. Chromatographic pattern of corticotropin fragments of Zorbax ODS sorbent].
The influence of acetonitrile concentration in the eluent and of the peptide hydrophobicity on the capacity factors has been studied. The equation is proposed that describes retention as a function of the eluent characteristics and the peptide composition. The hydrophobicity increments for -COOH, -NH2, and greater than CHCONH-fragments in the studied chromatographic system have been determined. The proposed model of peptide retention is useful for a prior evaluation of the eluent composition that is necessary to elute a compound at a given capacity factor. It can be also used for the qualitative interpretation of peptide chromatograms.
D000097:Acetonitriles / Q000032:analysis; D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007700:Kinetics; D010446:Peptide Fragments / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone; C032159:acetonitrile
Vysokoéffektivnaia zhidkostnaia khromatografiia peptidnykh bioreguliatorov, ikh fragmentov i proizvodnykh. I.Khromatograficheskoe povedenie fragmentov kortikotropina na sorbente Zorbax ODS.