title: Testicular changes observed in boars following experimental inoculation with pseudorabies (Aujeszky's) virus.
abstract: Four boars were inoculated intranasally with pseudorabies virus to determine if microscopic testicular changes occurred as a result of infection. Testicular biopsies and semen samples were taken at two, four and six weeks postinoculation and the boars were castrated immediately after the last sample collection. Testicular samples and semen were cultured to determine if the virus was present. Pseudorabies virus was not isolated from the semen or testicular tissue. Virus was isolated from trigeminal ganglia at necropsy and from nasal swabs taken one day after castration. Consequently, a time of high risk for shed of the virus from clinically normal carrier animals is immediately following castration. Gross changes were not observed in testicular tissues and microscopic changes in the testicles were the result of biopsy. Lesions consistent with pseudorabies virus infection were observed in the central nervous system of all inoculated boars. Temporary lowered fertility may result from the effects of elevated body temperature on spermatogenesis during acute clinical disease. However, it appears that the strain of pseudorabies virus used, lacked the ability to infect and/or replicate in the boars' reproductive tracts.
mesh_terms: D000818:Animals; D001706:Biopsy; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification; D008297:Male; D008854:Microscopy, Electron; D009296:Nasal Cavity / Q000382:microbiology; D011557:Pseudorabies / Q000382:microbiology / Q000473:pathology*; D012661:Semen / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000473:pathology*; D013737:Testis / Q000382:microbiology / Q000473:pathology*