[Interaction of n-alkanes with respiration and oxidative phosphorylation in rabbit heart mitochondria: n-dodecane, n-pentadecane and n-octadecane].
The effect of n-dodecane, n-pentadecane and n-octadecane at various concentrations (from 0 to 160 microgram/mg mitochondrial protein) and temperatures (from 15 degrees to 38 degrees C) is studied "in vitro" on coupled rabbit heart mitochondria. With both glutamate and succinate as substrates, and at higher temperatures only, n-dodecane makes the membrane permeable to protons (uncoupling), to some matrix enzymes and poorly to NADH; moreover it slightly inhibits NADH-oxidase and exerts a biphasic action (activation followed by inhibition) on succinate oxidase. The results, qualitatively similar to those observed with n-hexane and n-nonane, are yet less striking and exhibit features probably due to the longer chain. The other n-alkanes examined do not show any effect.
D000473:Alkanes / Q000494:pharmacology*; D000818:Animals; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008929:Mitochondria, Heart / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D011817:Rabbits; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013696:Temperature
D000473:Alkanes; D005971:Glutamates; D009097:Multienzyme Complexes; D013386:Succinates; D009243:NAD; C007548:n-dodecane; C033245:pentadecane; D018698:Glutamic Acid; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; C022883:octadecane
Interazioni di n-alcani con la respirazione e fosforilazione ossidativa di mitocondri cardiaci di coniglio: n-dodecano, n-pentadecano e n-octadecano.