[cAMP-dependent protein kinase from pigeon breast muscle. Isolation of regulatory subunits by affinity chromatography and study of the topography of the cAMP binding site using cAMP analogs].
The cAMP-dependent protein kinase from the soluble fraction of pigeon breast muscle is represented by two forms, PK I and PK II. The ratio of the phosphotransferase activity of the two forms is 35-40% and 60-65% for PK I and PK II, respectively. The regulatory subunit of PK I was isolated in a homogeneous state by affinity chromatography on 8-(2-oxoethylthio)-cAMP immobilized on epoxy-activated Sepharose 4B. The molecular weight of the regulatory subunit of PK I as determined by SDS polyacrylamide gel electrophoresis is 45 000. The specific cAMP-binding activity is equal to 16 nmol of [3H]cAMP per mg of protein. The apparent dissociation constant (Kd') for cAMP equals to 380 nM. The preparation of the regulatory subunit of PK II obtained by affinity chromatography on the same adsorbent is made up of polypeptides with Mr 56 000, 39 000, 29 000, 17 000 and 11 000. The preparation possesses a cAMP-binding activity of 22 nmol of [3H]cAMP per mg of protein. The interaction of several analogs of cAMP containing substituents at different positions of the nucleotide molecule with the regulatory subunit of PK I was studied. Practically all the analogs with substituents at positions 8 and 6 of the adenine ring in the cAMP molecule had the affinity which was 2-9 times less than that of cAMP. The only exceptions were 8-carboxymethylamino- and 8-(2-oxyethyl)-amino-cAMP whose binding to the regulatory subunit was 100 and 53 times lower than that of cAMP. The substitutions in position N-1 of the cAMP molecule leads to a 30-50-fold decrease of the analogs affinity. beta-Bromoethyl ester of cAMP does not reveal the ability to bind to the regulatory subunit. The carboxymethyl ester of cAMP possesses the affinity for the cAMP-binding site that is 35 times less than that of cAMP. Modification of the 2'-hydroxyl of ribose (as in the case of 2'-amino-2'-deoxy-8-hydroxy-cAMP, 2'-deoxy-cAMP and 2'-O-acrylyl-cAMP) decreases the affinity of these compounds 125-, 313- and 126-fold as compared with cAMP. It was assumed that the cAMP molecule is bound to the regulatory subunit in the syn-conformation. A structural model of the cAMP-binding site in the regulatory subunit of cAMP-dependent protein kinase I from pigeon breast muscle is proposed.
D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D010856:Columbidae; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008954:Models, Biological; D009132:Muscles / Q000201:enzymology* / Q000378:metabolism; D010664:Phenylmethylsulfonyl Fluoride; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D012710:Serum Albumin, Bovine
D007527:Isoenzymes; D012710:Serum Albumin, Bovine; D010664:Phenylmethylsulfonyl Fluoride; D000242:Cyclic AMP; D011494:Protein Kinases
cAMP-zavisimaia proteinkinaza iz grudnoĭ myshtsy golubia. Vydelenie reguliatornoĭ sub"edinitsy metodom affinnoĭ khromatografii i izuchenie topografii cAMP-sviazyvaiushchego tsentra s pomoshch'iu analogov cAMP.