Saturation behavior of single, amiloride-sensitive Na+ channels in planar lipid bilayers.
Single epithelial Na+ channels incorporated into planar lipid bilayers were studied to determine the effects of Na concentration on its own conductance. Amiloride-sensitive Na+ channels were obtained from apical membrane vesicles made from A6 cells, a continuous epithelial cell-line derived from amphibian kidney. Single-channel conductance was found to be a saturable function of Na+ concentration, with a Michaelis constant of approximately 17 or 47 mM, for a Gmax of approximately 4 or 44 pS, respectively.
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007668:Kidney; D008051:Lipid Bilayers; D011719:Pyrazines / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D014981:Xenopus
D007473:Ion Channels; D008051:Lipid Bilayers; D011719:Pyrazines; D000584:Amiloride; D012964:Sodium