A direct comparison between peroxisomal and mitochondrial preferences for fatty-acyl beta-oxidation predicts channelling of medium-chain and very-long-chain unsaturated fatty acids to peroxisomes.
A well-characterized crude peroxisomal fraction from brown adipose tissue was used to compare peroxisomal beta-oxidation with beta-oxidation in isolated mitochondria. The apparent Km and chain-length specificity for peroxisomal (acyl-CoA) and mitochondrial (acyl-carnitine) beta-oxidation were determined with saturated C4-C22 fatty acyls and some unsaturated fatty acyls. Peroxisomes showed the lowest Km for medium-chain (9:0-10:0) and mono-unsaturated long-chain (16:1-22:1) fatty acids, and highest oxidation rates with lauroyl-CoA (12:0). Mitochondria showed the lowest Km for long-chain fatty acids (16:0-18:0) and highest oxidation rates with 12:0-16:0 and with 18:2. These in vitro results offer an explanation of previous results obtained in situ by Foerster et al. (Foerster, E.-C., Fährenkemper, T., Rabo, U., Graf, P. and Sies, H. (1981) Biochem. J. 196, 705-712) and indicate a role for peroxisomes in degradation of medium-chain and mono-unsaturated long-chain fatty acids. It is concluded that no mechanism, other than relative preferences, needs to be suggested for channelling of fatty acids between the two subcellular organelles.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008830:Microbodies / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D005231:Fatty Acids, Unsaturated; D002374:Catalase; D003576:Electron Transport Complex IV