Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.
The Escherichia coli replication factor Y has been characterized as a phi X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003086:Bacteriocin Plasmids; D001665:Binding Sites; D004265:DNA Helicases / Q000378:metabolism*; D004261:DNA Replication; D010957:Plasmids; D013379:Substrate Specificity
D001426:Bacterial Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases