The effect of racemomycin-D, a nephrotoxic antibiotic, on cellular metabolism of rat kidney cortex in vitro.
In vitro effects of racemomycin-D on cellular metabolism were examined in rat kidney. Racemomycin-D decreased the concentration gradient of Na+ and K+ across the cell membranes, but failed to influence water content and ATP concentration of kidney cortical slices. The antibiotic inhibited microsomal (Na+ + K+)-ATPase. Preincubation of the microsomes with racemomycin-D enhanced the inhibition about 1.8-fold. Succinoxidase activity of mitochondria remained unaltered in the presence of racemomycin-D, but the antibiotic potently decreased ATP-dependent Ca2+ and Mg2+ uptakes by mitochondria. These results suggest that racemomycin-D probably disorders intracellular homeostasis of Na+, K+ and Ca2+.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013309:Streptothricins / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000187:drug effects; D010130:p-Aminohippuric Acid / Q000378:metabolism
D000900:Anti-Bacterial Agents; C019594:racemomycin; D013309:Streptothricins; D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium; D002118:Calcium; D010130:p-Aminohippuric Acid