Plasmid formation in Streptomyces: excision and integration of the SLP1 replicon at a specific chromosomal site.
We present data showing that the SLP1 plasmids found in Streptomyces lividans after mating with S. coelicolor strain A3(2) originate as deletion mutants of a 17 kb segment of the S. coelicolor chromosome. Excision of the entire 17 kb segment yields a transiently existing plasmid containing a site for integration into the chromosome of recipient SLP1- S. lividans strains at a unique locus that corresponds to the original chromosomal location of SLP1 in S. coelicolor. The deletion mutants of SLP1 lack the attachment site and/or other regions required for its integration, and thus persist in the recipient as autonomously replicating plasmids. Plasmids that contain the complete 17 kb sequence of the chromosomally integrated SLP1 segment were constructed in vitro by circularization of restriction endonuclease-generated fragments of chromosomal DNA carrying a tandemly-duplicated integrant of SLP1. Transformation of an SLP1- S. lividans strain with such plasmids results in chromosomal integration of the SLP1 sequence at the same site at which it is integrated in S. lividans cells that acquire the sequence by mating with S. coelicolor. A model for the site-specific excision and integration of SLP1 is presented.
D001483:Base Sequence; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000502:physiology*; D004262:DNA Restriction Enzymes; D005838:Genotype; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012093:Replicon; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics*
D004262:DNA Restriction Enzymes