[Generation of superoxides during the interaction of melanins with oxygen].
The rate of nitroblue tetrazolium (NBT) reduction by dihydroxyphenylalanine-melanin, pheomelanin and retinal pigment epithelium melanosomes under aerobic conditions (pH 7.4) is low both in the dark and upon illumination, but increases drastically in the presence of cetyltrimethylammonium bromide (CTAB). Under these conditions, the light insignificantly stimulates NBT reduction (1.3-fold). The reaction is effectively inhibited by superoxide dismutase. This suggests that superoxide anions (O2-. are formed as intermediate reaction products in the course of NBT reduction by melanins. At alkaline values of pH (greater than or equal to 9.0), the O2-.-dependent reduction of NBT can also take place in the absence of CTAB. In contrast with oxidation of photoreduced riboflavin, the melanin oxidation by O2 cannot induce lipid peroxidation. It is concluded that O2-. generation via melanin oxidation of melanosomes occurs only under non-physiological conditions and can hardly take place in vivo.
D000818:Animals; D002417:Cattle; D008543:Melanins / Q000378:metabolism*; D008544:Melanocytes / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010777:Photochemistry; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D008543:Melanins; C018362:pheomelanin; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D010100:Oxygen
Generatsiia superoksida pri vzaimodeĭstvii melaninov s kislorodom.