Propagation of large quantities of poliovirus in human lymphoblastoid cells grown in a serum-free medium.
All three types of poliovirus replicated to good titers in Epstein-Barr virus-transformed human lymphoblastoid cells isolated from patients with infectious mononucleosis. Cells were grown in a serum-free medium, which consisted of enriched Eagle's medium with addition of 0.1% albumin, 0.1% Intralipid and 1 mg/l of transferrin. Large quantities of virus could be produced in 2- to 20-l roller flasks or in spinner flasks. The propagated poliovirus gave an antibody response that complies with requirements for vaccine production.
D002460:Cell Line; D003470:Culture Media; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D017955:Poliovirus / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D003470:Culture Media