Nuclear magnetic resonance studies of blood platelets.
Blood platelets contain membrane-enclosed granules which have inside them high concentrations of 5-hydroxytryptamine (serotonin) along with adenine nucleotides and divalent metal ions. 19F n.m.r. of fluorinated serotonin incorporated into the granules of both human and pig intact platelets has shown that the motional state of the serotonin is restricted. Comparison with 31P n.m.r. experiments indicates that this restriction of motion is a consequence of high molecular weight aggregates formed by the adenine nucleotides and metal ions, and that it varies with the species from which the platelets are obtained. In the case of human platelet granules, at least, these high molecular weight aggregates are present in the absence as well as in the presence of serotonin. The biological significance of these data is briefly discussed.
D000227:Adenine Nucleotides / Q000097:blood; D000818:Animals; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012701:Serotonin / Q000097:blood; D013552:Swine
D000227:Adenine Nucleotides; D012701:Serotonin