Collagenase stimulation in cocultures of human fibroblasts and human tumor cells.
Cocultures of human fibroblasts with 3 different human tumor cell lines exhibited high levels of collagenase activity against type I collagen compared to individual cultures of each cell type. For LX-1 lung carcinoma cells maximum stimulation occurred at a ratio of tumor cells to fibroblasts of 0.5:1. These cocultures contained 15-50 times more collagenase activity than the sum of the individual cultures. Conditioned medium from the LX-1 cells stimulated the fibroblasts to an extent similar to that obtained in the cocultures. Conditioned medium from the fibroblasts did not stimulate the tumor cells. Thus human tumor cells secrete a factor(s) which stimulates collagenase production by human fibroblasts.
D000818:Animals; D001943:Breast Neoplasms / Q000503:physiopathology*; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000503:physiopathology*; D051379:Mice; D008819:Mice, Nude; D003012:Microbial Collagenase / Q000378:metabolism*; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000503:physiopathology*; D012867:Skin / Q000201:enzymology*; D014183:Transplantation, Heterologous
D003012:Microbial Collagenase