Perchloric acid treatment and use of chromogenic substrate in the Limulus test: application to veterinary diagnosis.
The Limulus test was carried out for endotoxin determination using a synthetic chromogenic substrate after perchloric acid(PCA) treatment to remove inhibiting substances in the blood. PCA treatment completely removed non-specific amidolytic activity and other factors which interfered with the Limulus test. The recovery of added endotoxin was about 100% irrespective of animal species. Endotoxin levels in PCA-treated blood plasma of healthy domestic animals were invariably below 1 pg ml-1. Many of the cows with gangrenous mastitis, from which Gram-negative rods were isolated, showed high endotoxin levels ranging from 22.8 to 138.0 pg ml-1. Endotoxin was also detected in the blood of cows affected with salmonellosis, colibacillosis, Haemophilus somnus infection and urethritis associated with Proteus.
D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002645:Chickens / Q000097:blood; D002863:Chromogenic Compounds; D004731:Endotoxins / Q000097:blood*; D005260:Female; D006736:Horses / Q000097:blood; D006358:Hot Temperature; D008033:Limulus Test / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000175:diagnosis; D010472:Perchlorates; D013552:Swine / Q000097:blood
D002863:Chromogenic Compounds; D004731:Endotoxins; D010472:Perchlorates