Cyclic AMP inhibits the growth of human breast cancer cells in defined medium.
Three well-characterized human breast cancer cell lines, MCF-7, T47D and Cama-1, have been grown in defined medium in the absence of serum. Under these conditions, the growth of these cells was inhibited by a variety of cyclic AMP elevating agents, including cholera toxin, monobutyryl cyclic AMP, bromo-cyclic AMP, prostaglandin E2, and 1-methyl-3-isobutyryl xanthine. This inhibitory effect of cyclic AMP on breast cancer cells contrasts with the stimulation of proliferation induced by elevation of intra-cellular cyclic AMP in normal human mammary epithelial cells. Such a major perturbation of growth control may arise either as a result of malignant change, or may reflect a difference in the differentiation phenotype of normal and malignant human mammary epithelial cells in vitro.
D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics
D002772:Cholera Toxin; D000242:Cyclic AMP