Terminal structures of F2, a linear DNA plasmid from yeast: two different terminal structures in one linear DNA molecule.
The terminal structure of F2, a linear double-stranded DNA plasmid of 3.9 kb from yeast, was analyzed. Results obtained by electrophoretic analyses of terminal restriction fragments of F2 showed that one end of F2 has terminal protein at the 5'-terminus, but another end has hairpin structure. This is a novel type of autonomously replicating DNA molecule.
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal; D010957:Plasmids; D004718:Saccharomycetales / Q000235:genetics*
D004271:DNA, Fungal; D004262:DNA Restriction Enzymes