title: Locations and sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC): the tRNAGly contains only two base-pairs in the D stem.
abstract: The nucleotide sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC) have been determined. None of these genes contains an intron. One unusual feature is that the tRNAGly contains only two base-pairs (A-U, G-U) in the D stem. These four tRNA genes were located in the known physical map of tobacco chloroplast DNA. Hybridization analysis to chloroplast tRNA revealed that all four tRNA genes are transcribed in vivo.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002736:Chloroplasts / Q000032:analysis*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D012343:RNA, Transfer / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics; D012358:RNA, Transfer, Met; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D012343:RNA, Transfer; D004262:DNA Restriction Enzymes