Isolation and bacterial expression of a murine alpha leukocyte interferon gene.
A murine alpha interferon gene (MuIFN-alpha A) was isolated from a murine genomic DNA library in phage lambda. The gene was cloned and its sequence determined. It was expressed in Escherichia coli under the control of the trp promoter/operator which resulted in antiviral activity on mouse L-cells. MuIFN-alpha A exhibits 60% homology to HuIFN-alpha A at the amino acid level.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D009876:Operon
D007370:Interferon Type I