title: Codon usage can affect efficiency of translation of genes in Escherichia coli.
abstract: By inserting synthetic oligonucleotides into a highly expressed gene in E. coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein. However, in the case of the codon used (AGG), a significant effect on translation was only seen at very high transcription rates from a gene containing multiple copies of the unfavourable codon.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005686:Galactokinase / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D007700:Kinetics; D009690:Nucleic Acid Conformation; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D012333:RNA, Messenger; D005686:Galactokinase; D004262:DNA Restriction Enzymes