Intraparticulate localization of some peroxisomal enzymes related to fatty acid beta-oxidation.
Male Wistar rats were given a diet containing 0.05% (w/w) LK-903 (alpha-methyl-p-myristyroxycinnamic acid 1-monoglyceride) for 2 weeks. The activities of four hepatic peroxisomal enzymes involved in the fatty acyl-CoA beta-oxidizing system were determined. The activities of fatty acyl-CoA oxidase, crotonase, beta-hydroxybutyryl-CoA dehydrogenase and thiolase were all increased about three times by administration of LK-903. The intraparticulate localizations of the four enzymes were then investigated by treatment of the purified peroxisomes with Triton X-100, by sonication, and by sucrose-density-gradient centrifugation after Triton X-100 treatment. The results suggest that thiolase is localized in the matrix of peroxisomes, that crotonase and beta-hydroxybutyryl-CoA dehydrogenase are located in the core, and that all or at least part of fatty acyl-CoA oxidase is associated with the core, though its association is weak.
D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D042962:Acyl-CoA Oxidase; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002934:Cinnamates / Q000494:pharmacology; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005637:Fructuronate Reductase / Q000378:metabolism; D005989:Glycerides / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D017830:Octoxynol; D009940:Organoids / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013010:Sonication
D000924:Anticholesteremic Agents; D002934:Cinnamates; D005227:Fatty Acids; D005989:Glycerides; C017974:methyl-p-myristyloxycinnamic acid 1-monoglyceride; D011092:Polyethylene Glycols; D017830:Octoxynol; D010088:Oxidoreductases; D005637:Fructuronate Reductase; D042962:Acyl-CoA Oxidase; D000101:Acetyl-CoA C-Acetyltransferase; D004746:Enoyl-CoA Hydratase