Sensitivity of mitomycin C and nitrogen mustard crosslinks to extreme alkaline conditions.
DNA-DNA crosslinks in cells treated with mitomycin C, nitrogen mustard, or decarbamoyl mitomycin C were measured in alkaline isopycnic gradients as a function of pH. Crosslinks from cells treated with mitomycin C and nitrogen mustard, which react with DNA purines, could be detected at pH 12.5 but not at pH 14. No crosslinks from cells treated with decarbamoyl mitomycin C were detected at either pH. Previous studies with cells exposed to psoralen derivatives plus 360 nm light, which produce DNA-DNA crosslinks with pyrimidines, demonstrated stable crosslinks at pH 14. These studies indicate that DNA-DNA crosslinks involving DNA purines are much less stable at high pH than those involving pyrimidines, and that methods involving exposure to extreme alkaline conditions may give inaccurate information for some agents.
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002500:Centrifugation, Isopycnic; D004247:DNA / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008466:Mechlorethamine / Q000494:pharmacology*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D013539:Simian virus 40
D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D008466:Mechlorethamine; D016685:Mitomycin; D004247:DNA