Release of somatostatin from extra-hypothalamic rat brain slices: inhibition by dopamine and morphine.
Calcium-dependent potassium-evoked release of somatostatin-like immuno-reactivity (SSLI) and preloaded [3H]noradrenaline ([3H]NA) could be demonstrated simultaneously from slices of rat cerebral cortex, globus pallidus and trigeminal nucleus. The release of [3H]NA from cortical slices differed from that of somatostatin (SS) in its K+-dependency, with the release of SS having a higher threshold. Both morphine (10 microM) and dopamine (50 microM) significantly inhibited the potassium-evoked release of SS from the cerebral cortex, without affecting its basal release. The effect of morphine was naloxone reversible.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D046508:Culture Techniques; D004298:Dopamine / Q000378:metabolism*; D004573:Electrolytes / Q000494:pharmacology; D005917:Globus Pallidus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000378:metabolism*; D014278:Trigeminal Nuclei / Q000378:metabolism
D004573:Electrolytes; D013004:Somatostatin; D009020:Morphine; D004298:Dopamine; D009638:Norepinephrine