Resolution and identification of the "subunits" of cytochrome C oxidase from mouse liver.
Mouse liver cytochrome c oxidase was purified to a specific heme a content of 9.3 nmol/mg protein and the constituent polypeptides separated on SDS-polyacrylamide gel electrophoresis. Using this system, the enzyme was resolved into 12 to 14 polypeptide bands.
D000818:Animals; D003576:Electron Transport Complex IV / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006418:Heme / Q000032:analysis; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D008930:Mitochondria, Liver / Q000201:enzymology*; D013367:Submitochondrial Particles / Q000201:enzymology
D046911:Macromolecular Substances; D006418:Heme; D003576:Electron Transport Complex IV