Microassay of gamma-glutamyl transpeptidase in needle biopsies of human liver.
A method is described for determination of liver gamma-GTP activity in homogenates of the small amount of human liver tissues (approx. 5--7 mg wet wt.) obtained by needle biopsy. The gamma-GTP activity in the liver of healthy subject was < 20 mU/mg protein. The microdetermination of gamma-GTP described here is worth in the diagnosis of hepatic injury, especially alcoholic hepatitis and drug-induced liver diseases.
D000814:Aniline Compounds / Q000378:metabolism; D001707:Biopsy, Needle; D003124:Colorimetry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008107:Liver Diseases / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000032:analysis* / Q000097:blood
D000814:Aniline Compounds; D005723:gamma-Glutamyltransferase