Effect of levamisole on lymphocyte blastogenesis and neutrophil function in dexamethasone-treated cattle.
Levamisole was evaluated at 6 dose levels for its ability to prevent the dexamethasone-induced suppression of in vitro lymphocyte blastogenesis or neutrophil function in cattle. Dexamethasone (0.4 mg/kg of body weight, IM) and levamisole hydrochloride (0.5, 1.0, 2.0, 4.0, or 8.0 mg/kg orally) were administered to groups of 4 cattle daily for 3 days. Another group of 4 cattle were given the 3-day dexamethasone treatment and 6.0 mg/kg of levamisole (the recommended anthelmintic dose) was given only once on the 1st day that dexamethasone was given. Results obtained from the dexamethasone-levamisole-treated cattle were compared with results obtained from cattle that were given only dexamethasone. Levamisole had no apparent consistent ability to enhance lymphocyte blastogenic responsiveness (to the mitogens phytohemagglutinin, concanavalin A, or pokeweed mitogen or in a 1-way mixed lymphocyte reaction) or to enhance neutrophil function (random migration, nitroblue tetrazolium reduction, iodination, or antibody-dependent cell-mediated cytotoxicity) in dexamethasone-treated cattle.
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D002417:Cattle / Q000276:immunology*; D002464:Cell Migration Inhibition; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007108:Immune Tolerance / Q000187:drug effects; D007978:Levamisole / Q000008:administration & dosage / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology
D008934:Mitogens; D007978:Levamisole; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone