Effects of anaerobiosis and nitrate on the expression of succinate dehydrogenase and enzymes associated with nitrogen metabolism in Klebsiella pneumoniae.
We have shown that the low histidase activity found in anaerobic, nitrogen-limited cultures of Klebsiella pneumoniae is due to repression of the right-hand hut operon. In addition, we have examined the effects of NO3- on the aerobic and anaerobic expression of catabolite- and NH4+-repressible enzymes in this organism. NO3- permitted anaerobic growth of K. pneumoniae in minimal medium containing histidine as the sole carbon source, and histidase and succinate dehydrogenase were derepressed during anaerobic growth in histidine/NO3- medium. Use of sucrose rather than histidine as the carbon source reversed the effects of NO3- and repressed histidase and succinate dehydrogenase activities. Anaerobic growth in sucrose/NO3- medium also uncoupled the expression of urease and glutamine synthetase.
D000693:Anaerobiosis; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006638:Histidine Ammonia-Lyase / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D014510:Urease / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D009566:Nitrates; D013395:Sucrose; D013385:Succinate Dehydrogenase; D001616:beta-Galactosidase; D014510:Urease; D006638:Histidine Ammonia-Lyase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen