Immunoreactive dynorphin-(1-8) and corticotropin- releasing factor in subpopulation of hypothalamic neurons.
Immunoreactive corticotropin-releasing factor (CRF) and dynorphin-(I-8) were visualized in rat hypothalamus by immunohistofluorescence with specific antibodies. In brains from colchicine-treated, adrenalectomized rats, neuronal perikarya with immunoreactive CRF were observed in the paraventricular nucleus of the hypothalamus. The CRF occurred together with the dynorphin-(1-8). However, the CRF immunoreactivity occurred only in a subpopulation of the dynorphin-(1-8) immunoreactive cells. These findings suggest that there may be a functional interrelationship of CRF with dynorphin-related opioid peptides and provide further evidence that neurons may contain more than one bioactive substance.
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000276:immunology / Q000378:metabolism*; D004399:Dynorphins; D004723:Endorphins / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D051381:Rats
D004723:Endorphins; D010446:Peptide Fragments; D004399:Dynorphins; D003346:Corticotropin-Releasing Hormone