Effect of free fatty acids and cholesterol in vitro on liver plasma membrane-bound enzymes.
The effect of cholesterol and fatty acid treatment in vitro was tested on rat liver plasma membrane-bound enzymes and lipid fluidity. The observed alterations of membrane fluidity affect both (Na+-K+)-ATPase and Mg2+-ATPase activities but not 5'-nucleotidase; basal adenylate cyclase as well as its hormonal sensitivity were differentially affected by changes of membrane microenvironment.
D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002462:Cell Membrane / Q000201:enzymology; D002784:Cholesterol / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005454:Fluorescence Polarization; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D002784:Cholesterol; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000262:Adenylyl Cyclases; D000254:Sodium-Potassium-Exchanging ATPase