Thymus cell migration: cells migrating from thymus to peripheral lymphoid organs have a "mature" phenotype.
To gain information on the lineage relationship of cells leaving the thymus, we studied the phenotype of thymus emigrants within hours of their exit. The migrants were identified in the peripheral lymphoid organs by their fluorescence, 3 to 4 hr after intrathymic injection of a solution of fluorescein isothiocyanate, a technique that initially only labels thymocytes. Migrants identified in this way were analyzed with rhodamine-anti-Thy-1 or rhodamine peanut agglutinin (PNA). They were found to express Thy-1 antigen and PNA binding sites at levels very similar to those found on the majority of peripheral T cells or medullary thymocytes and quite different from cortical thymocytes. Taken together with our previous experiments on Lyt-1, Lyt-2, and H-2 levels, the data show that cells leaving the thymus are quite mature in phenotype and are indistinguishable from peripheral T cells by all the criteria examined.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation; D003348:Cortisone / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D037102:Lectins / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019887:Peanut Agglutinin; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell; D011975:Receptors, Mitogen; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000166:cytology*; D014916:Whole-Body Irradiation
D000954:Antigens, Surface; D037102:Lectins; D008565:Membrane Proteins; D019887:Peanut Agglutinin; D011948:Receptors, Antigen, T-Cell; D011975:Receptors, Mitogen; D018800:Thy-1 Antigens; D003348:Cortisone