Nuclear transplantation in mouse embryos.
The ability of foreign nuclei to support development in nuclear transplantation manipulations has proven an effective means to assess the consequences of nuclear differentiation. In addition, nuclear transplantation might serve to define the persistence and role of maternally inherited cytoplasmic constituents during embryogenesis. We have extended the use of a technique that enables the efficient transfer of one-cell-stage pronuclei into the cytoplasm of enucleated mouse embryos, and have successfully transferred two-, four-, eight-cell-stage and inner cell mass (ICM) cell nuclei. We have also used this technique as a means to determining that the stage-specific embryonic antigen, SSEA-3, is a cytoplasmic contribution of the unfertilized ovum. The potential value of this technique in determining the developmental capacity of nuclei from various embryonic states, and in determining nuclear/cytoplasmic origins or early embryonic gene products, is discussed.
D000818:Animals; D002467:Cell Nucleus / Q000276:immunology; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000276:immunology / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis / Q000235:genetics; D016256:Lewis X Antigen; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008846:Micromanipulation; D008866:Microsurgery; D053652:Nuclear Transfer Techniques; D011247:Pregnancy
D006017:Glycolipids; D016256:Lewis X Antigen