title: Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system.
abstract: Twenty-three rat lung specimens collected in outbreaks of hemorrhagic fever with renal syndrome (HFRS) in three medical institutions were inoculated onto the VERO-E6 cell monolayers. After several blind passages, an agent growing serially in the cell cultures and reacting specifically with known HFRS-positive sera was isolated from two of these specimens. The two isolates were antigenically identical each other. The agent, named strain SR-11, was identified as the causative virus of HFRS by its antigenic identity with E6 cell-adapted HFRS virus, Hantaan 76-118 strain, and the specific reactions with sera from various HFRS cases.
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D007564:Japan; D007674:Kidney Diseases / Q000382:microbiology; D008297:Male; D012328:RNA Viruses / Q000302:isolation & purification*; D051381:Rats; D013577:Syndrome
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral