The Salmonella/mammalian-microsome assay: variations of the test protocol; results of a questionnaire returned by 87 laboratories.
The answers to a questionnaire sent to 147 laboratories in Europe using the Salmonella/mammalian-microsome assay are presented. In mutagenicity testing, where research projects in environmental mutagenesis predominate, the Salmonella/mammalian-microsome assay is the most often used test. Of all laboratories that answered the questionnaire, 80% are using the test with occasional modifications (preincubation, fluctuation test). Most of the differences concern the activation system used. This questionnaire is part of a European collaborative study which aims to improve the standardization of the Salmonella/mammalian-microsome assay.
D000818:Animals; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D051381:Rats; D012475:Salmonella / Q000235:genetics; D011795:Surveys and Questionnaires