Quantitative determination of the beta-adrenoceptor stimulant formoterol in urine by gas chromatography mass spectrometry.
A method for the quantitative determination of the beta-stimulant formoterol in urine, using a gas chromatograph--mass spectrometer, is described. Formoterol can be analyzed after the addition of a deuterium-labelled internal standard and conversion to a mixed bis-pentafluoropropionyl-methyl derivative for selected ion monitoring. The detection limit was 5 ng/ml. Urinalysis after the oral administration of formoterol fumarate, using a combined enzymic hydrolysis method, revealed that the drug was conjugated with glucuronic acid in rats, dogs and humans.
D000318:Adrenergic beta-Agonists / Q000652:urine*; D000818:Animals; D004285:Dogs; D004983:Ethanolamines / Q000008:administration & dosage / Q000378:metabolism / Q000652:urine*; D000068759:Formoterol Fumarate; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000378:metabolism; D006801:Humans; D008297:Male; D051381:Rats; D012016:Reference Values
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D005965:Glucuronates; D000068759:Formoterol Fumarate