Effects of neurotransmitter candidates on 45Ca uptake by cortical slices of rat brain: stimulatory effect of L-glutamic acid.
The effects of neurotransmitter candidates and the characteristics of the stimulatory effect of L-glutamic acid (L-Glu) on 45Ca uptake by rat brain slices were investigated. 45Ca uptake was significantly stimulated by acetylcholine, serotonin and especially L-Glu, but not by other neurotransmitter candidates. L-Glu caused dose-dependent stimulation of 45Ca uptake (L-Glu-stimulated 45Ca uptake), its effect being half-maximal at 1 microM. The related compounds D-glutamic acid, D,L-alpha-aminoadipic acid and N-methyl-D,L-glutamic acid (final conc. of 10 microM) also stimulated 45Ca uptake, but less than 10 microM L-Glu. D,L-alpha-Methylglutamic acid and L-glutamic acid diethylether (final conc. of 10 microM), which are specific inhibitors of L-Glu, inhibited L-Glu-stimulated 45Ca uptake. Mg,Ca-ATPase activity was hardly affected by a concentration of 10 microM L-Glu that caused maximal stimulation of 45Ca uptake. These findings suggest that L-Glu-stimulated 45Ca uptake by brain cortical slices is linked to L-Glu receptor.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004298:Dopamine / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D005971:Glutamates; D018377:Neurotransmitter Agents; D012701:Serotonin; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D006632:Histamine; D000109:Acetylcholine; D002118:Calcium; D004298:Dopamine; D009638:Norepinephrine