[Effect of cycloheximide on lysosomal proteinase activity in rat organs].
Administration of cycloheximide (0.1-0.4 mg/kg bw) to rats caused a progressive drop of cathepsin A, B, C and D activity in the liver and kidneys. In the spleen, the activity of all lysosomal proteinases declined 50-70% even with the minimal dose of cycloheximide. On the contrary, the activity of other lysosomal hydroxylases (beta-galactosidase and arylsulfatases A and B) dropped by not more than 10-25% in all the organs under study, regardless of the fact that the dose of cycloheximide was maximal. It is suggested that the reduction of cathepsin activities during protein biosynthesis inhibition is a regulatory mechanism by which the protein resources in the cell are preserved.
D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D002553:Cerebroside-Sulfatase / Q000378:metabolism; D002803:Chondro-4-Sulfatase / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism
D003513:Cycloheximide; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase; D001616:beta-Galactosidase; D002403:Cathepsins; D010450:Endopeptidases
Vliianie tsiklogeksimida na aktivnost' lizosomnykh proteinaz razlichnykh organov krys.