Influence of myosin antiserum on heart sarcolemmal Ca2+ or Mg2+ ATPase activity.
Sarcolemmal Ca2+/Mg2+ ATPase was inhibited only about 30% by myosin antiserum that decreased myofibrillar ATPase activity by about 80%. There was a remarkable difference in the effect of myosin antiserum on sarcolemmal Ca2+/Mg2+ ATPase and myofibrillar ATPase with regard to its pre-incubation time with these organelles. Tryptic digestion of the sarcolemmal membrane did not show any change in its inhibitory effect of the myosin antiserum on Ca2+ ATPase. The data distinguish the Ca2+/Mg2+ ATPase from myosin ATPase and suggest that it is an enzyme of the sarcolemmal origin.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004285:Dogs; D007106:Immune Sera; D066298:In Vitro Techniques; D009206:Myocardium / Q000201:enzymology*; D009210:Myofibrils / Q000201:enzymology; D009218:Myosins / Q000276:immunology*; D012508:Sarcolemma / Q000201:enzymology*; D013997:Time Factors
D007106:Immune Sera; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D009218:Myosins; D000252:Calcium-Transporting ATPases