The effect of dibutyryl cyclic guanosine monophosphate, a competitive antagonist to cholecystokinin-pancreozymin, on gastric acid secretion in the isolated mouse stomach.
Dibutyryl cyclic guanosine monophosphate (dbcGMP), a specific competitive inhibitor of the gastrin, cholecystokinin-pancreozymin (CCK-PZ) family of peptides in pancreas, gallbladder and ileum, had no effect on basal acid secretion in the isolated mouse stomach nor on secretion stimulated by bethanechol or histamine. Secretion evoked by low doses of pentagastrin were likewise unaffected by dbcGMP but responses to high doses of pentagastrin were augmented. CCK-PZ and glucagon each inhibited acid secretion evoked by pentagastrin. DbcGMP blocked CCK-PZ-mediated inhibition but was without effect on inhibition by glucagon. These observations suggest that in the gastric glands there exist two receptors with different affinities for gastrin and CCK-PZ which mediate excitation and inhibition respectively.
D000818:Animals; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology; D002766:Cholecystokinin / Q000037:antagonists & inhibitors*; D006152:Cyclic GMP / Q000031:analogs & derivatives*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000037:antagonists & inhibitors; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010418:Pentagastrin / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000187:drug effects; D011949:Receptors, Cholecystokinin; D013270:Stomach / Q000187:drug effects*
D001626:Bethanechol Compounds; D005755:Gastrins; D011956:Receptors, Cell Surface; D011949:Receptors, Cholecystokinin; D018723:Bethanechol; D003995:Dibutyryl Cyclic GMP; D006632:Histamine; D002766:Cholecystokinin; D010418:Pentagastrin; D006152:Cyclic GMP