The human carcinoma cell line A431 possesses large numbers of functional beta-adrenergic receptors.
The existence of beta-adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1-[3H]dihydroalprenolol and the hydrophilic antagonist [3H]CGP-12,177 as beta-adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the beta 2-subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human beta-adrenergic receptors.
D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D001667:Binding, Competitive; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D004082:Dihydroalprenolol / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000378:metabolism; D011412:Propanolamines / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D011433:Propranolol; D007545:Isoproterenol; C033394:CGP 12177