beta-Adrenergic response in mouse CNS reaggregate cultures.
Primary reaggregate cultures from 17-day embryonic mouse brains were prepared and tested for beta -adrenergic response. Isoproterenol stimulated the production of cAMP 3- to 4-fold over basal levels after 10 days in culture. The response to isoproterenol was rapid, reaching a maximum 1-2 min after stimulation, and was blocked by propranolol. The beta-adrenergic receptor was characterized in membranes prepared from reaggregates by measurement of specific binding of [125I]iodohydroxybenzylpindolol (IHYP). The Kd for IHYP was 100-300 pM and did not change as a function of time in culture. A maximum receptor density of approximately 80-110 fmol/mg membrane protein was found. Both beta-adrenergic receptors and response appeared at times in culture, which were approximately equivalent to birth.
D000318:Adrenergic beta-Agonists / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000032:analysis*; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; C011878:iodohydroxybenzylpindolol; D010869:Pindolol; D000242:Cyclic AMP; D007545:Isoproterenol