A plasma membrane Mg2+-ATPase in the cellular slime mold Dictyostelium discoideum.
Evidence is presented for the existence of a plasma membrane ATPase in Dictyostelium discoideum. The enzyme is dependent on Mg+2, and is insensitive to both azide and oligomycin. It is, however, sensitive to diethylstilbestrol, dicyclohexylcarbodiimide, vanadate, and thimerosal. Monovalent cations (Na+, K+, and choline) have no effect on enzyme activity, but high concentrations of Ca+2 are highly inhibitory. Vegetative cells express the highest amount of enzyme activity; the activity decreases three- to fourfold during the early stages of differentiation, and then remains constant during the latter stages.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology; D017301:Ca(2+) Mg(2+)-ATPase; D002462:Cell Membrane / Q000201:enzymology*; D004023:Dictyostelium / Q000201:enzymology* / Q000254:growth & development; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology; D009711:Nucleotides / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D013849:Thimerosal / Q000494:pharmacology; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D001386:Azides; D009711:Nucleotides; D009840:Oligomycins; D014639:Vanadium; D013849:Thimerosal; D014638:Vanadates; D004024:Dicyclohexylcarbodiimide; D004054:Diethylstilbestrol; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase