title: Adenosinetriphosphatase (Mg-ATPase) activity in the plasma membrane of preimplantation mouse embryo as revealed by electron microscopy.
abstract: The ultrastructural localization of Mg2+-ATPase activity was investigated in unfertilized eggs and in preimplantation mouse embryos. Enzyme activity was detected on the entire surface of unfertilized eggs and in one-cell embryos. From the two-cell stage up to the 12-cell embryo the reaction product was localized both at the exposed surface of the embryo and between blastomeres. At the late morula stage, activity was only present on the free surface of the peripheral cells outside the apical tight junctions. In the early blastocyst the membranar reaction was observed both on the outside membrane of the trophoblastic cells and on the inner surface facing the blastocoele, whereas in the late blastocyst the reaction remained unchanged on the outer surface of the embryo but was absent from the membranes bordering the blastocoele. These results are discussed with reference to the modification of the cell surface membrane during preimplantation development.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D001755:Blastocyst / Q000201:enzymology* / Q000648:ultrastructure; D017301:Ca(2+) Mg(2+)-ATPase; D002454:Cell Differentiation; D002462:Cell Membrane / Q000201:enzymology*; D006651:Histocytochemistry; D007365:Intercellular Junctions; D051379:Mice; D008854:Microscopy, Electron; D009028:Morula / Q000201:enzymology
chemical_list: D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase