Synergistic enzyme induction by glucocorticoids and cyclic AMP observed in glioma x hepatoma cell hybrids but not in their parents.
Enzyme induction by hydrocortisone (HC) and dibutyryl cyclic AMP (dbcAMP) was studied in C6 rat glioma cells, FU5AH rat hepatoma cells, and five C6 x FU5AH hybrids. Hormone responsive enzymes from both parental lines were studied, including: tyrosine aminotransferase (TAT), alanine aminotransferase (AAT), glycerol phosphate dehydrogenase (GPDH), lactate dehydrogenase (LDH), and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP). There was no overall dominance of one parental phenotype over the other in expression of uninduced or induced enzyme activity after fusion, and the hybrids possessed some enzymatic properties characteristic of both parents. GPDH was induced by dbcAMP in all five hybrids, and TAT was induced by dbcAMP in four of the hybrids, although neither of these enzymes were induced by dbcAMP in the parents. Furthermore, synergistic induction of these enzymes by HC and dbcAMP was observed in the hybrids but not in the parents. These hybrids provide a model system to study hormone interaction in enzyme induction.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000096:biosynthesis; D000410:Alanine Transaminase / Q000096:biosynthesis; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D004357:Drug Synergism; D004790:Enzyme Induction; D005910:Glioma / Q000201:enzymology*; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis; D006822:Hybrid Cells / Q000187:drug effects / Q000201:enzymology*; D006854:Hydrocortisone / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis
D003994:Bucladesine; D000242:Cyclic AMP; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D014444:Tyrosine Transaminase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D006854:Hydrocortisone