title: The Golgi apparatus and lysosomes of rat pancreatic acinar cells following refeeding.
abstract: The short term effects of refeeding on the Golgi apparatus and lysosomes of the rat exocrine pancreas were evaluated by ultrastructural, morphometric and cytochemical methods. Ten minutes after refeeding, there was a significant enlargement of Golgi cisternae and a significant increase, compared with the controls, in the number of condensing vacuoles and lysosomes. These modifications were accompanied by the appearance of acid phosphatase activity in stacked Golgi cisternae (as well as GERL) of some cells. One hour after refeeding, there were about the same numbers of condensing vacuoles and lysosomes as in the controls; Golgi cisternae were still significantly enlarged, compared with the controls, but they were no longer reactive for acid phosphatase. In both fasting and refed animals, acid phosphatase activity was demonstrable in tubular lysosomes. The data are interpreted in terms both of membrane disposal and recycling, leading to enhanced formation of zymogen granules, during physiologically stimulated secretion.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure; D005215:Fasting; D005502:Food; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006651:Histocytochemistry; D008247:Lysosomes / Q000648:ultrastructure*; D008297:Male; D010179:Pancreas / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013834:Thiamine Pyrophosphatase / Q000032:analysis; D013997:Time Factors
chemical_list: D000135:Acid Phosphatase; D013834:Thiamine Pyrophosphatase