A method for the isolation of the caribbean palytoxin (C-PTX) from the coelenterate (zooanthid) Palythoa caribaeorum.
Chromatographically pure toxin was isolated from the zooanthid Palythoa caribaeorum. Toxin isolation was achieved by extraction with 50% ethanol from the homogenized specimens, gel filtration on Sephadex G-50 and ion exchange chromatography on QAE- and SP-Sephadex. Final purification was obtained by gel filtration on Biogel P-6. The LD50 tested on the shore crab Carcinus maenas was 62.5 ng/kg.
D000178:Acrylamides; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003063:Cnidaria / Q000032:analysis*; D003064:Cnidarian Venoms / Q000302:isolation & purification* / Q000633:toxicity; D000095702:Polyether Toxins
D000178:Acrylamides; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins