Characteristics of the binding of colchicine to porcine brain, cerebellum, pancreas, kidney, liver and spleen soluble protein: a comparative study.
Quantitative determination of tubulin levels present in the cytosol of different porcine organs was performed by a sensitive radioimmunoassay. The relationship between the amount of protein from liver or spleen and the colchicine bound was curvilinear, possibly due to the presence of an inhibitor of colchicine-tubulin binding in those organs. The rate of decay of colchicine binding increases when protein concentration decreases in brain, cerebellum, kidney and pancreas but it increases when protein concentration increases in liver and spleen.
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002531:Cerebellum / Q000378:metabolism; D003078:Colchicine / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D011485:Protein Binding; D011863:Radioimmunoassay; D013154:Spleen / Q000378:metabolism; D013552:Swine; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
D014404:Tubulin; D003078:Colchicine