[Subcellular and submitochondrial localization of inorganic pyrophosphatase in the mammalian heart and liver].
The rat heart and liver homogenates contain 3 forms of inorganic pyrophosphatase designated as I, II and III in accordance with a decrease of their electrophoretic mobility. Separation of the homogenates by stepped density gradient differential centrifugation demonstrated that forms I and II are localized in the hyaloplasm, while form III --in the mitochondria. Liver mitochondria contain in addition some amount of pyrophosphatase II. The hyaloplasm and mitochondria of bovine heart contain 2 enzyme forms, whose mobilities are identical to those of forms I and II. Upon destruction of rat mitochondria by detergent (digitonin, Lubrol WX) treatment, sonication or osmotic shock followed by a freezing-thawing precedure pyrophosphatase II remains bound to the inner membrane fragments. The experimental data provide direct evidence for the existence of a specific membrane pyrophosphatase in the mitochondria.
D000818:Animals; D002417:Cattle; D043564:Inorganic Pyrophosphatase; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D008929:Mitochondria, Heart / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity; D011755:Pyrophosphatases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013367:Submitochondrial Particles / Q000201:enzymology*
D007527:Isoenzymes; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase
Subkletochnaia i submitokhondrial'naia lokalizatsiia neorganicheskoĭ pirofosfatazy v serdtse i pecheni mlekopitaiushchikh.