Immunoglobulin A and secretory immunoglobulin A antibodies to purified type 1 Klebsiella pneumoniae pili in human colostrum.
Immunoglobulin A antibodies with binding specificity for purified Klebsiella pneumoniae type 1 pili were detected by an enzyme-linked immunosorbent assay in 20 of 21 human samples (95%). The concentrations of secretory immunoglobulin A antibody in colostrum directed against the pili were calculated by comparison of experimental enzyme-linked immunosorbent assay values with values obtained from known secretory immunoglobulin A concentrations. The presence of antibodies to K. pneumoniae type 1 pili was confirmed by double diffusion-gel studies with selected specimens of colostrum. This study shows that in the majority of human colostral samples examined, secretory immunoglobulin A antibodies with specificity for K. pneumoniae type 1 pili can be commonly found in variable, but frequently high, concentrations.
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D002458:Cell Fractionation; D003126:Colostrum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007711:Klebsiella pneumoniae / Q000276:immunology* / Q000648:ultrastructure
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory