Galactosyltransferase defects in reeler mouse brains.
Galactosyltransferase activities were examined in the cerebellum, cerebral cortex, and brain stem of reeler and wild-type mice. Galactosyltransferase assays were optimal for all required substrates, linear with incubation time, and proportional to protein concentration. In brain areas affected by the reeler mutation (i.e., cerebral cortex and cerebellum), galactosylation of both endogenous and exogenous glycoprotein acceptors was greatly reduced in reeler relative to controls. On the other hand, glycosylation of endogenous glycolipids was low, and equal between reeler and wild-type. Galactosyltransferase activities were similar, though not identical, in reeler and wild-type brain stems, which are phenotypically normal in reeler mice. Glucosyltransferase, beta-galactosidase, beta-N-acetylglucosaminidase, acid phosphatase, and lactate dehydrogenase specific activities were all unaffected in reeler cerebella, while galactosyltransferase activity was 52% of control. Inhibition of either UDPgalactose hydrolysis or beta-galactosidase had no effect on galactosyltransferase activity. The spectrum or galactosyltransferase deficiencies in reeler suggests that this enzyme is associated with the development of young granule cells.
D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000254:growth & development; D001933:Brain Stem / Q000201:enzymology; D002531:Cerebellum / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D005700:Galactosyltransferases / Q000378:metabolism*; D051379:Mice; D008818:Mice, Neurologic Mutants; D011755:Pyrophosphatases / Q000378:metabolism; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000378:metabolism*
D014531:Uridine Diphosphate Galactose; D005700:Galactosyltransferases; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase; D001616:beta-Galactosidase; D011755:Pyrophosphatases; C021899:nucleotide pyrophosphatase; D000117:Acetylglucosamine