Selective activation of synapses near the tip of drug-ejecting microelectrode, and effects of antagonists of excitatory amino acids in the hippocampus.
A technique was developed to selectively facilitate transmission through synapses near the tip of a drug-ejecting micropipette by increasing Ca2+ concentration in a small space surrounding the tip. By means of this technique, we found that antagonists of excitatory amino acids, cis-2,3-piperidine dicarboxylic acid, gamma-D-glutamylglycine and glutamic acid diethylester, blocked excitation induced in CA3 neurons by glutamate and by mossy fiber stimulation in thin hippocampal sections of the guinea pig.
D000596:Amino Acids / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004151:Dipeptides / Q000494:pharmacology; D004336:Drug Antagonism; D005071:Evoked Potentials / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D008839:Microelectrodes; D010875:Pipecolic Acids / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology*
D000596:Amino Acids; D004151:Dipeptides; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D010875:Pipecolic Acids; C011829:glutamic acid diethyl ester; C028929:gamma-glutamylglycine; D018698:Glutamic Acid; C029262:2,3-piperidinedicarboxylic acid; D002118:Calcium