Effect of several xenobiotics on the activities of enzymes affecting ascorbic acid synthesis in rats.
The dietary addition of several xenobiotics, such as PCB, DDT, aminopyrine, chloretone, BHT and BHA, caused significant increases in the ascorbic acid in urine and liver of rats. The administration of all types of xenobiotics used in the present experiments increased the activity of hepatic UDP-glucose dehydrogenase (1.3-2.8-fold), and the administration of PCB, DDT, BHT or BHA significantly increased the activity of hepatic UDP-glucuronyl transferase (2.2-13.1-fold). The activity of beta-glucuronidase was slightly increased with feeding of PCB, DDT, chloretone or aminopyrine. However, the activity of hepatic UDP-glucuronic acid pyrophosphatase, the conversion of D-glucuronic acid or D-glucuronolactone into L-ascorbic acid and the activity of hepatic L-gulonolactone oxidase did not increase with the administration of PCB or DDT. It is suggested that the increases in the activities of UDP-glucose dehydrogenase and UDP-glucuronyl transferase would have a major role in the stimulation of ascorbic acid synthesis in xenobiotic treated rats.
D000632:Aminopyrine / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002724:Chlorobutanol / Q000494:pharmacology; D003634:DDT / Q000494:pharmacology; D005965:Glucuronates / Q000378:metabolism*; D020723:Glucuronic Acid; D005966:Glucuronidase / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D050544:L-Gulonolactone Oxidase; D008099:Liver / Q000378:metabolism*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D011755:Pyrophosphatases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism
D005965:Glucuronates; D000632:Aminopyrine; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D020723:Glucuronic Acid; D003634:DDT; D011078:Polychlorinated Biphenyls; D013401:Sugar Alcohol Dehydrogenases; D014533:Uridine Diphosphate Glucose Dehydrogenase; D050544:L-Gulonolactone Oxidase; D014453:Glucuronosyltransferase; D005966:Glucuronidase; D011755:Pyrophosphatases; C023259:UDP glucuronate pyrophosphatase; D002724:Chlorobutanol; D001205:Ascorbic Acid