title: The interaction of lymphocyte membrane proteins with the lymphocyte cytoskeletal matrix.
abstract: Lymphocyte membrane proteins are important in the transduction of signals across the plasma membrane. Visual and biophysical studies have shown that after ligand binding, membrane proteins may become immobile in the plane of the membrane and may cap. In intact cells, binding of cross-linking ligands to surface immunoglobulin converts it to a detergent-insoluble state (77% insoluble). This conversion is positively correlated with the transmission of a mitogenic signal. Class II histocompatibility proteins (Ia) and thy-1 remain predominantly detergent soluble (60 to 97% soluble). Insolubilized membrane proteins may be solubilized by incubating the detergent insoluble cytoskeletons with 0.34 M sucrose, 0.5 mM ATP, 0.5 mM dithiothreitol, 1 mM EDTA, or 3 X 10(-5) M DNAase I, 1 mM EDTA. To determine if the membrane-associated cytoskeleton contains the sufficient components for ligand-induced receptor insolubilization, experiments were done with a crude plasma membrane fraction. The results with whole cells or crude plasma membranes were comparable. These studies support the view that ligand-induced insolubilization of membrane proteins is due to their interaction with cytoskeletal structures.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D000954:Antigens, Surface / Q000032:analysis; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D011946:Receptors, Antigen / Q000032:analysis / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D012995:Solubility; D013154:Spleen / Q000166:cytology; D018800:Thy-1 Antigens
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins; D011946:Receptors, Antigen; D011947:Receptors, Antigen, B-Cell; D018800:Thy-1 Antigens