Hepatic morphine and estrone glucuronyltransferase activity and morphine biliary excretion in the isolated perfused rat liver. Effect of pregnancy and estradiol-17 beta treatment.
Hepatic microsomal estrone and morphine glucuronyltransferase activity and the biliary excretion of morphine in the isolated perfused liver were examined in nonpregnant, pregnant (19-21 days of gestation), and estradiol-17 beta-treated (E2; 1.0 mg/kg/day sc for 14 days) rats. Pregnancy decreased estrone and morphine glucuronyltransferase activity 20%, whereas E2 treatment increased activity 50%. Treatment of nonpregnant and pregnant rats with 2,3,7,8-tetrachlorodibenzo-p-dioxin (3 micrograms/kg po) increased estrone glucuronyltransferase activity 1.3- and 2.8-fold respectively, but such treatment had no effect in E2-treated rats. In the isolated perfused liver, E2 treatment, but not pregnancy, decreased the biliary excretion of morphine 3-glucuronide. Bile flow (microliter/min/g of liver) was slightly decreased by pregnancy but not by E2 treatment. Maximal bile/perfusate concentration ratios of morphine glucuronide were 175, 325, and 90 in livers from nonpregnant, pregnant, and E2-treated rats, respectively.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology*; D004970:Estrone / Q000378:metabolism*; D005260:Female; D014453:Glucuronosyltransferase / Q000032:analysis*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology*; D009020:Morphine / Q000378:metabolism*; D009022:Morphine Derivatives / Q000378:metabolism; D010477:Perfusion; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal; D051381:Rats; D011919:Rats, Inbred Strains
D009022:Morphine Derivatives; D000072317:Polychlorinated Dibenzodioxins; D004970:Estrone; D004958:Estradiol; D009020:Morphine; D014453:Glucuronosyltransferase; C018108:morphine-3-glucuronide