title: Technical notes on acrylamide gel electrophoresis used for comparing isozymes of mosquito larvae.
abstract: Various slight modifications of electrophoretic techniques were tried to obtain more reproducible results for comparing several isozymes with individual mosquito larvae. Horizontal electrophoresis was done using polyacrylamide gel plates of 1 mm thickness on a simple cooling system in which ice-cold water was circulated. This made it possible to carry out electrophoresis effectively even during field work in tropical areas. Gel concentration in routine work is 5% for usual enzymes, but for esterase, 6% gels have been adopted. Gel concentrations, running distance, running time, visualization of isozyme bands, etc., have especially been considered.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D009033:Culicidae / Q000145:classification / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007814:Larva; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase