A rapid screening test for antenatal sex determination.
A recombinant DNA probe was used for antenatal sex determination. The probe was isolated from the 3.4 kilobase human repeat sequence derived from heterochromatin of the Y chromosome and had 1000 times more affinity for male DNA than for female DNA. A dot blot hybridisation method with 200 microliter of amniotic fluid yielded accurate results in 2 to 3 days. This test should facilitate antenatal diagnosis of sex-linked genetic disorders.
D000653:Amniotic Fluid / Q000166:cytology; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism / Q000494:pharmacology; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012732:Sex Determination Analysis; D013997:Time Factors; D014998:Y Chromosome / Q000032:analysis
D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases