title: Distribution of cadmium, copper and zinc in the liver of spot salamander, Ambystoma maculatum and their binding to metallothionein.
abstract: Cadmium (Cd) was injected intramuscularly into male spot salamanders, Ambystoma maculatum. Concentrations and contents of Cd, zinc (Zn), copper (Cu), iron, magnesium and calcium in the livers of control and Cd-injected animals were determined simultaneously. A low molecular weight Cd, Cu, Zn-binding protein present in the hepatic supernatant prepared from control and Cd-injected animals was characterized as metallothionein (MT) from several properties characteristic of MT. MT induced and present in the liver supernatants of spot salamanders consisted of a single isoform.
mesh_terms: D000557:Ambystoma / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002104:Cadmium / Q000008:administration & dosage / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D015032:Zinc / Q000378:metabolism
chemical_list: D008670:Metals; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium