title: Calcium and the mechanism of axoplasmic transport.
abstract: Using desheathed cat peroneal nerves in in vitro studies, Ca2+ was recently shown to be required to maintain axoplasmic transport. Calmodulin was also shown to be present in nerve and to participate in transport. These findings open up new possibilities for a better understanding of the underlying mechanism of transport. In the transport filament model, the materials transported are bound to a common carrier, the transport filaments, which are moved along the microtubules by means of an interaction with the side arms of the microtubules. This is an energy-requiring process that depends on a supply of ATP, which is utilized by the Ca2+,Mg2+-ATPase associated with the side arms of the microtubules. The Ca2+,Mg2+-ATPase is activated by calmodulin at the low micromolar levels of free Ca2+ present in the axon. The level is kept low by calcium-regulatory mechanisms that include mitochondria, endoplasmic reticulum, and calcium-binding proteins. Nerves exposed to higher-than-normal concentrations of Ca2+ in the medium show an increased number of particles in these organelles as expected of their Ca2+-regulatory role. The nature of the calmodulin-Ca,Mg-ATPase complex associated with the side arms is discussed on the basis of the transport model. Also discussed is slow transport, which is explained on the basis of the model as a differential binding affinity to the transport filaments.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001693:Biological Transport, Active; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002415:Cats; D003599:Cytoskeleton / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological
chemical_list: D002147:Calmodulin; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium