Isolation and in vitro translation of a mammalian protamine mRNA.
mRNA was isolated from sexually mature rat, rabbit, and bovine testes. Poly(A+) and (A-) RNAs were prepared and hybridized to a rainbow-trout protamine probe. The bovine (A+) fraction showed significant hybridization compared to the other species and these related sequences were also found in total bovine DNA. Bovine mRNA programmed the in vitro synthesis of a basic protein that possessed protamine-like properties. The mRNA was fractionated by agarose-gel electrophoresis and the fractions hybridized to the trout protamine probe. A significant hybridization signal was observed corresponding to a mRNA of approximately 400 nucleotides in length which coded for the protamine-like protein. The data support the view that we have isolated a mammalian (bovine) protamine mRNA.
D000818:Animals; D002417:Cattle; D066298:In Vitro Techniques; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification; D011479:Protamines / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013737:Testis / Q000378:metabolism
D011479:Protamines; D012333:RNA, Messenger; D011061:Poly A