High-performance liquid chromatographic analysis of analogous amino and oxo acids for the determination of amino acid oxidase and transaminase activities.
An analytical method, based on reversed-phase high-performance liquid chromatography, for the determination of analogous amino and oxo acids, has been developed. The assay may be employed to monitor amino acid oxidase and transaminase activities. Simultaneous quantitative determination of substrate and product is readily achieved. In particular, the amino acid oxidase activity within whole microbial cells immobilized by entrapment in Ca2+-alginate has been studied. The assay of transaminases has been exemplified with glutamic-pyruvic transaminase.
D000410:Alanine Transaminase / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D051263:D-Alanine Transaminase; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D045782:Hemiterpenes; D007651:Keto Acids / Q000032:analysis*; D008715:Methionine / Q000031:analogs & derivatives / Q000032:analysis; D000637:Transaminases / Q000378:metabolism*
D000596:Amino Acids; D045782:Hemiterpenes; D007651:Keto Acids; C014632:2-keto-4-methylthiobutyric acid; C001505:alpha-ketoisovalerate; D008715:Methionine; D003605:D-Amino-Acid Oxidase; D000637:Transaminases; D000410:Alanine Transaminase; D051263:D-Alanine Transaminase