Autoradiographic studies on the proliferation of glomerular and tubular cells of the rat kidney in early diabetes.
Although Osterby and coworkers have shown that the onset of diabetes is associated with glomerular hypertrophy and enlargement of the filtration surface, the pathogenesis of this lesion and the behaviour of the glomerular tuft cells during the increase in glomerular filtration surface area remain unclear. Autoradiography and electron microscopic morphometry in kidneys of male Sprague-Dawley rats with streptozotocin-induced diabetes indicate that glomerular enlargement is combined with glomerular cell hyperplasia. 6 days after continuous 3H-thymidine infusion there is a significant increase in DNA-synthesis in tuft cells indicating cell proliferation. Since podocyte volume only increases in acute diabetes (as shown by Osterby and Gundersen in 1980), and the mean podocyte nuclear area remains unchanged, we conclude that the increased DNA-synthesis results in podocyte hypercellularity so that an increased glomerular filtration surface area can be covered by a greater number of cells.
D000818:Animals; D001345:Autoradiography; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D006984:Hypertrophy; D007678:Kidney Glomerulus / Q000473:pathology / Q000648:ultrastructure*; D007684:Kidney Tubules / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D004247:DNA