title: Dynorphin in bovine adrenal medulla. I. Detection in glandular and cellular extracts and secretion from isolated chromaffin cells.
abstract: The presence and secretion of immunoreactive dynorphin in bovine adrenal medulla and isolated adrenal chromaffin cells were examined and compared with those of immunoreactive leucine-enkephalin. Using an antiserum raised against dynorphin-(1-13), a sensitive and highly specific radioimmunoassay was developed. A nearly intact antigen was required for recognition by the antiserum since it did not react with leucine-enkephalin and reacted poorly with dynorphin-(1-12) (cross-reactivity 0.5%) and other fragments of dynorphin-(1-13). On the other hand, the antibody used for detection of leucine-enkephalin did not cross-react with dynorphin(1-13). Adrenal medulla acid extracts contained 195 times more immunoreactive leucine-enkephalin than dynorphin. However, the concentration of immunoreactive dynorphin in acid extract of freshly isolated adrenal chromaffin cells was only 1.4 times smaller than that of leucine-enkephalin. Incubation of the isolated chromaffin cells in the presence of acetylcholine, nicotine, high potassium, but not muscarine, induced a concomitant release of immunoreactive dynorphin (3.5-9% of total cell content) and leucine-enkephalin (6.5-11.4% of total cell content).
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004399:Dynorphins; D004723:Endorphins / Q000276:immunology / Q000378:metabolism*; D004743:Enkephalin, Leucine / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology; D010446:Peptide Fragments / Q000276:immunology; D011189:Potassium Chloride / Q000494:pharmacology; D011863:Radioimmunoassay
chemical_list: D004723:Endorphins; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; D011189:Potassium Chloride; D009538:Nicotine; C026843:dynorphin (1-13); D004399:Dynorphins; D000109:Acetylcholine