Regulation of expression of liver-specific enzymes. I. Detection in mammalian tissues and cultured cells.
Nineteen enzymes showing highest activity in liver were examined in human and rodent tissues and cultured cells using starch-gel electrophoresis. The rat hepatoma line Faza 967 strongly expressed 13 of these enzymes. A series of somatic cell hybrids, constructed between Faza and cells of non-hepatic origin derived from man or from Chinese hamster, were examined for expression of these enzymes. Some of the human/rat hybrids continued to produce rat liver-specific enzymes, and the human forms of the enzymes glutamate-pyruvate transaminase, alpha-glycerophosphate dehydrogenase, alcohol dehydrogenase and pyruvate kinase L were reexpressed in a few cases.
D000444:Aldehyde Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000818:Animals; D001124:Argininosuccinate Synthase / Q000235:genetics; D002460:Cell Line; D006224:Cricetinae; D004592:Electrophoresis, Starch Gel; D006597:Fructose-Bisphosphatase / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics; D008099:Liver / Q000201:enzymology*; D051379:Mice; D051381:Rats; D014444:Tyrosine Transaminase / Q000235:genetics
D007527:Isoenzymes; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D014444:Tyrosine Transaminase; D006597:Fructose-Bisphosphatase; D001124:Argininosuccinate Synthase