title: [Enzyme activity of thiamine diphosphate biosynthesis and degradation in the mouse liver in the dynamics of B1 avitaminosis development].
abstract: Activities of thiamin pyrophosphokinase (TPK EC 2.7.6.2) and thiamin pyrophosphatase (TPPase EC 3.6.1.6) were studied in liver homogenate supernatants obtained from mouse (female, body weight of 21-23 g) in dynamics of B1 avitaminosis. As compared with controls distinct deficiency of thiamin led a decrease in coenzyme thiamin diphosphate (TPP) down to 15% as well as of transketolase activity (TPP-dependent enzyme) down to 35%, especially during the terminal stage of the avitaminosis; the process was accompanied by cyclic alterations in activity of TPK and TPPase. Within 9 and 17 days of the B1 avitaminosis the activity of TPK increased by 62% and 38% and the activity of TPPase--decreased by 30% and 20%, respectively. Alterations in the enzymatic activity appear to be characteristic for adaptation in response to thiamin deficiency.
mesh_terms: D000818:Animals; D005260:Female; D008099:Liver / Q000201:enzymology*; D051379:Mice; D013836:Thiamin Pyrophosphokinase / Q000378:metabolism; D013832:Thiamine Deficiency / Q000378:metabolism*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000378:metabolism*; D013997:Time Factors; D014174:Transketolase / Q000378:metabolism
chemical_list: D014174:Transketolase; D013836:Thiamin Pyrophosphokinase; D013834:Thiamine Pyrophosphatase; D013835:Thiamine Pyrophosphate
vernacular_title: Aktivnost' fermentov biosinteza i degradatsii tiamindifosfata v pecheni mysheĭ v dinamike razvitiia B1-avitaminoza.