Isolation and partial characterization of a lethal sea nettle (Chrysaora quinquecirrha) mesenteric toxin.
A protein lethal to mice was isolated from the sea nettle (Chrysaora quinquecirrha) mesenteric tentacle nematocysts by immunochromatography. The partially purified protein was also toxic to spontaneously beating cultural chick cardiocytes but was not hemolytic to rat erythrocytes. On sodium dodecyl sulfate polyacrylamide gels, the mesenteric protein consisted of three bands with apparent molecular weights of 175,000, 160,000 and 108,000. The three protein bands yielded similar peptide maps when subjected to limited proteolysis.
D000818:Animals; D002642:Chick Embryo; D003064:Cnidarian Venoms / Q000302:isolation & purification* / Q000633:toxicity; D004586:Electrophoresis; D006321:Heart / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments
D003064:Cnidarian Venoms; D010446:Peptide Fragments; C031441:sea nettle toxin