Derepression of nitrogenase by addition of malate to cultures of Rhodospirillum rubrum grown with glutamate as the carbon and nitrogen source.
Rhodospirillum rubrum grown in continuous culture with glutamate as the sole fixed C and N source produced no nitrogenase, and the cultures were characterized by high extracellular ammonium concentrations. Addition of organic acids derepressed nitrogenase. Glutamate dehydrogenase, glutamine synthetase, glutamate synthase, malate dehydrogenase, nitrogenase, and ammonium were assayed before and after malate addition.
D000641:Ammonia / Q000032:analysis; D004794:Enzyme Repression / Q000187:drug effects; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000378:metabolism; D008293:Malates / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism; D009591:Nitrogenase / Q000096:biosynthesis*; D012247:Rhodospirillum rubrum / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D005971:Glutamates; D008293:Malates; D018698:Glutamic Acid; D000641:Ammonia; C030298:malic acid; D008291:Malate Dehydrogenase; D009591:Nitrogenase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen