Localization of tyrosine-hydroxylase immunoreactive cells in rabbit retinal cultures.
Tyrosine-hydroxylase immunoreactivity is demonstrable by the indirect immunofluorescence technique in a small sub-population of retinal neurones cultured from 2-day-old rabbits. Staining was only weak, restricted to a few neurones, and could not be observed in cultures of less than 10 days. Autoradiographical analysis of [3H]dopamine uptake by retinal cultures strongly suggests that neurones containing tyrosine-hydroxylase immunoreactivity have the capacity to take up exogenous dopamine. These findings are in concordance with studies on rabbit retinal preparations.
D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D011817:Rabbits; D012160:Retina / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase