Identification of an Mr 77,000 - 80,000 product of in vitro translation of rat liver mRNA using antibody to glycogen synthase.
Rabbit antibody to rat liver glycogen synthase has been used to identify a product of Mr 77,000 - 80,000 from in vitro translation of rat liver mRNA. A comparison of various protease inhibitors on the relative molecular weight of rat liver glycogen synthase suggest that higher molecular weight enzyme forms could arise from incomplete hydrolysis of glycogen before enzyme isolation and enzyme subunit Mr determinations.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D006006:Glycogen Synthase / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism
D000906:Antibodies; D000936:Antigen-Antibody Complex; D012333:RNA, Messenger; D011061:Poly A; D006006:Glycogen Synthase