Hormones and the differentiation of type A spermatogonia in mouse cryptorchid testes incubated in vitro.
In order to study hormonal effects on testicular germ cell differentiation, especially on type A spermatogonia, artificially induced cryptorchid testes of adult mice were cultured in a medium containing testosterone, dihydrotestosterone, tri-iodothyronine, dibutyryl 3':5' cyclic adenosine monophosphate, human chorionic gonadotrophin, LH, FSH, insulin and transferrin. These substances, with the exception of FSH, showed no stimulatory effect on the differentiation of type A spermatogonia. However, FSH activated cell division in type A spermatogonia and stimulated them to differentiate, while LH showed neither the promotion of differentiation nor a synergistic effect on FSH-mediated germ cell differentiation.
D000728:Androgens / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003456:Cryptorchidism / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D013093:Spermatogonia / Q000187:drug effects / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D000728:Androgens; D006728:Hormones; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone