Neurotransmitter-synthesizing enzymes in 14 human neuroblastoma cell lines.
Fourteen human neuroblastoma cell lines were studied for expression and regulation of neurotransmitter-synthesizing enzymes. All cell lines contained activities of adrenergic and/or cholinergic neurons and 13 expressed activities for both. None contained enzymes for serotonergic or GABAergic neurons. Enzyme activity was characteristic for a given cell line. Enzyme activity in cell lines was sensitive to growth phase, culture medium, and concentration of fetal bovine serum.
D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma / Q000201:enzymology* / Q000503:physiopathology; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D018377:Neurotransmitter Agents; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases