title: Characterization of enkephalins and related peptides in rat hypophysial portal blood.
abstract: Rat hypophysial portal blood, collected from the pituitary stalk, was extracted and enkephalins were assayed by different RIA. Met-Enk-IR and Leu-Enk-IR levels were 1635 +/- 470 pg/ml and 125 +/- 50 pg/ml, respectively. Using HPLC characterization, the presence in portal blood of Met-Enk, Leu-Enk, proenkephalins fragments and dynorphin1-17 has been demonstrated. An unidentified Met-Enk-IR peptide has also been found.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000097:blood; D004743:Enkephalin, Leucine / Q000097:blood; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000097:blood; D004745:Enkephalins / Q000097:blood*; D008297:Male; D010446:Peptide Fragments / Q000097:blood; D010902:Pituitary Gland / Q000098:blood supply*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004745:Enkephalins; D010446:Peptide Fragments; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; C026843:dynorphin (1-13); C027511:enkephalin-Met, Arg(6)-Phe(7)-; D004399:Dynorphins