Determination of the psychostimulants pemoline, fenozolone and thozalinone in human urine by gas chromatography/mass spectrometry and thin layer chromatography.
Detection of the psychostimulants pemoline (Tradon), fenozolone (Ordinator) and thozalinone (Stimsen) in biological samples (urine) presents certain experimental difficulties. These substances were therefore converted by acid hydrolysis to their common hydrolysis product 5-phenyl-2,4-oxazolidinedione, which can be detected with high sensitivity and selectivity by thin layer chromatography. After silylation with N-methyl-N-trimethylsilyltrifluoroacetamide/trimethylchlorosilane quantitative results can be obtained from the same extract by the proposed GC/MS-method. After a single dose of 30 mg pemoline, levels ranging from one to four mg/l urine were observed.
D000697:Central Nervous System Stimulants / Q000652:urine; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010080:Oxazoles / Q000652:urine*; D010389:Pemoline / Q000031:analogs & derivatives* / Q000652:urine*; D014297:Trimethylsilyl Compounds
D000697:Central Nervous System Stimulants; D010080:Oxazoles; D014297:Trimethylsilyl Compounds; C004996:fenozolone; C034173:thozalinone; D010389:Pemoline