title: Enzyme clusters during the metamorphic period of Ambystoma mexicanum: role of thyroid hormone.
abstract: Enzyme activities and DNA content have been measure in axolotl liver during the metamorphic period (4-8 months after spawning). Three different types of enzyme activity profiles were observed. In the type I profile (carbamoyl-phosphate synthase, arginase, ornithine transcarbamoylase, and glutamate dehydrogenase) enzyme activity is high in the youngest animals studied, and shows a minimum at 5 months followed by a maximum at 8 months of age. Thereafter activities do not change or slightly decrease. In the type II profile (tyrosine aminotransferase, glucose-6-phosphatase) enzyme activity shows a peak at 5 months of age and is low thereafter. Hexokinase, the enzyme with a type III profile, shows high activity throughout the metamorphic period. DNA content remains high throughout the metamorphic period but decreases 50% between 9 and 12 months of age, probably due to an increase in the size of the hepatocytes. No glucokinase activity was detected. High activities of cluster II enzymes represent early metamorphic events, while the rising part of cluster I is associated with late metamorphic events. The apparent molecular specific activity increases during natural development between 5 and 9 months of age, or precociously, upon thyroid hormone treatment. This change in apparent molecular specific activity is correlated to the advent of ureotelism.
mesh_terms: D000375:Aging; D000557:Ambystoma / Q000502:physiology*; D000558:Ambystoma mexicanum / Q000502:physiology*; D000818:Animals; D001119:Arginase / Q000378:metabolism; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008675:Metamorphosis, Biological; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D014284:Triiodothyronine / Q000502:physiology*; D014444:Tyrosine Transaminase / Q000378:metabolism
chemical_list: D014284:Triiodothyronine; D004247:DNA; D005969:Glutamate Dehydrogenase; D009954:Ornithine Carbamoyltransferase; D014444:Tyrosine Transaminase; D006593:Hexokinase; D005952:Glucose-6-Phosphatase; D001119:Arginase; D002222:Carbamoyl-Phosphate Synthase (Ammonia)