Purification of tick-borne encephalitis virus by affinity chromatography using monoclonal antibody.
The application of a simple technique for purification of tick-borne encephalitis (TBE) virus is described. TBE virus was grown in chick embryo cell (CE) cultures and the virus was concentrated by differential centrifugation. Final purification was made by the filtration through Sepharose column to which monoclonal antibodies to TBE virus had been bound. The method was effective in eliminating avian retroviruses.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002642:Chick Embryo; D002846:Chromatography, Affinity / Q000379:methods*; D003604:Czechoslovakia; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology / Q000302:isolation & purification*; D006386:Hemagglutination Tests; D011863:Radioimmunoassay; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral