Purification of pili and outer membrane vesicles of Neisseria gonorrhoeae by wheat germ agglutinin affinity chromatography.
Previous studies indicated that OMVs of Neisseria gonorrhoeae reacted specifically with WGA. A technique was therefore developed for the separation of gonococcal pili and OMVs by WGA affinity chromatography. This method was more convenient and more effective than isopycnic centrifugation for obtaining OMVs free of pili. The antigens were further purified but though homogeneous as judged by in vitro analytical methods, they both elicited an antibody response in animals to minor impurities previously undetected.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000648:ultrastructure; D007123:Immunoelectrophoresis, Two-Dimensional; D037102:Lectins; D009344:Neisseria gonorrhoeae / Q000276:immunology / Q000648:ultrastructure*; D014909:Wheat Germ Agglutinins
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D037102:Lectins; D014909:Wheat Germ Agglutinins