Supramolecular relationships of membrane antigens on the murine thymocyte.
The topographical relationships of Thy-1, Ly-1, Ly-2, and T200 were examined on the murine thymocyte. The inhibition of the binding of radiolabelled monoclonal antibody after incubation with unlabelled heterologous antibody was used as a measure of the proximity of the target antigens on the cell surface. On unfixed cells, prior incubation with anti-Thy-1.2 impeded the attachment of labelled anti-Ly-1, anti-Ly-2, and anti-T200. Anti-T200, but not anti-Ly-1, or anti-Ly-2, was capable of impeding the attachment of labelled anti-Thy-1.2 on unfixed cells. In general, gentle fixation with paraformaldehyde did not alter these inter-relationships. The possible functional contributions of such supramolecular relationships are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000954:Antigens, Surface; D001667:Binding, Competitive; D003601:Cytotoxicity Tests, Immunologic; D005404:Fixatives; D066298:In Vitro Techniques; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000954:Antigens, Surface; D005404:Fixatives; D018800:Thy-1 Antigens