Adjuvant-free immunological manipulation of livestock.
Mice and sheep were immunised to growth hormone release inhibiting hormone (GHRIH) using a number of systems which avoided conventional adjuvants. GHRIH was linked directly, or via a horse IgG carrier, to algin or purified protein derivative of tuberculin. A conjugate of GHRIH and flagella from Salmonella dublin was also prepared. Complexes were administered with or without lipopolysaccharide from S typhosa 0901 in a two-injection schedule. Animals receiving conjugates containing purified protein derivative or flagella were preimmunised with live Bacille Calmette-Guérin (BCG) or live S dublin vaccines respectively. Antibody titres to GHRIH, horse IgG and flagella were determined by radioimmunoassay. The system based on tuberculin yielded anti-GHRIH titres in both species which were equivalent to those obtained using Freund's complete adjuvant, while the system based on S dublin produced similar results but only in mice. These data suggest that carrier proteins of bacterial origin may be useful in the development of adjuvant-free autoimmunisation schedules for the practical manipulation of endocrine systems.
D000464:Alginates / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D005407:Flagella / Q000276:immunology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006736:Horses / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice / Q000276:immunology*; D008815:Mice, Inbred Strains; D012475:Salmonella / Q000276:immunology; D012756:Sheep / Q000276:immunology*; D013004:Somatostatin / Q000276:immunology*; D013045:Species Specificity; D014373:Tuberculin / Q000276:immunology
D000464:Alginates; D001323:Autoantibodies; D006603:Hexuronic Acids; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D014373:Tuberculin; D013004:Somatostatin; D020723:Glucuronic Acid