Long-term effects of dexamethasone and nerve growth factor on adrenal medullary cells cultured from young adult rats.
Normal postnatal rat chromaffin cells and rat pheochromocytoma cells are known to show extensive Nerve Growth Factor (NGF)-induced process outgrowth in culture, and this outgrowth from the postnatal chromaffin cells is abolished by the corticosteroid dexamethasone. To determine whether adult rat chromaffin cells respond to NGF and dexamethasone, dissociated adrenal medullary cells from 3-month-old rats were cultured for 30 days in the presence or absence of these agents. Such cultures contained typical chromaffin cells, chromaffin cells with processes, and neurons. Fewer than 2% of normal adult chromaffin cells formed processes under any of the conditions studied, and statistically significant changes in this proportion were not detectable in the presence of NGF or dexamethasone. Adrenal medullary neurons, however, were observed only in the presence of NGF, in cultures with or without dexamethasone, and thus appear to be previously unreported NGF targets which require NGF for survival or process outgrowth. Dexamethasone markedly increased total catecholamine content, total content of epinephrine, and tyrosine hydroxylase activity in cultures with or without NGF. In contrast, postnatal rat chromaffin and rat pheochromocytoma cells which have been studied in culture do not produce epinephrine under any of these conditions. It is concluded that rat adrenal chromaffin cells undergo age-related changes in both structural and functional plasticity. The in vitro characteristics of rat pheochromocytoma cells more closely resemble those of postnatal than of adult rat chromaffin cells, but may not entirely reflect the properties of the majority of chromaffin cells in either age group.
D000310:Adrenal Gland Neoplasms / Q000503:physiopathology; D000313:Adrenal Medulla / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002478:Cells, Cultured; D002837:Chromaffin Granules / Q000648:ultrastructure; D003907:Dexamethasone / Q000494:pharmacology*; D004837:Epinephrine / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000503:physiopathology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism; D010673:Pheochromocytoma / Q000503:physiopathology; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D009414:Nerve Growth Factors; D003907:Dexamethasone; D014446:Tyrosine 3-Monooxygenase; D009638:Norepinephrine; D004837:Epinephrine