Activity of acetoacetyl--CoA thiolase and regulation of ketone body metabolism in the brain of the developing chick.
The acetoacetyl-CoA thiolase activity was estimated in subcellular fractions isolated from the cerebral hemispheres, the optic lobes and the cerebellum of the chick between the 20th day of embryonic life and the 30th day of postnatal maturation. Acetoacetyl-CoA thiolase is located both in mitochondria and microsomes of the chick brain. Mitochondrial enzyme activity remains high between the 20th day of embryonic life and the 2nd or the 4th day after hatching, depending on the considered brain area. It then decreases until 30 days after hatching. Cytoplasmic thiolase activity remains unchanged during pre- and postnatal development in the cerebral hemispheres; it increases during the same time in the optic lobes. In the cerebellum, cytoplasmic thiolase activity develops in the same way as in mitochondria. The regulation of ketone body utilization by the developing chick brain widely differs from that by the mammalian brain. In the chick, 3-hydroxybutyrate is nearly the single ketone body utilized by the brain. It is converted into acetyl-CoA in the mitochondria and cytoplasm of the chick brain through two exactly parallel pathways involving the participation of the same enzymes.
D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism*; D000123:Acetyltransferases / Q000378:metabolism*; D000375:Aging; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D002531:Cerebellum / Q000201:enzymology; D002642:Chick Embryo; D002645:Chickens; D003576:Electron Transport Complex IV / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology; D003336:Tectum Mesencephali / Q000201:enzymology; D014018:Tissue Distribution
D007657:Ketone Bodies; D003576:Electron Transport Complex IV; D000123:Acetyltransferases; D000101:Acetyl-CoA C-Acetyltransferase