Alloantigenic phenotype of radiation-induced thymomas in the mouse.
The alloantigenic phenotype of 21 radiation-induced thymomas is described. Monoclonal antibodies and conventional antisera, absorbed to remove contaminating antibodies, were used to test the thymomas directly for the presence of H-2, Ia, Ly-1, Ly-2, Ly-4, Ly-5, Ly-6, Ly-9, Ly-15, Thy-1, TL, and Qa-2 antigens, and for surface immunoglobulin. The phenotypes obtained by direct tests were also confirmed by absorption studies. The tumors were all of T-cell origin (Thy-1+Ig-) and showed a restricted heterogeneity of their cell surface phenotype, concordant with the known alloantigenic phenotypes of functional T-cells. Thus the thymomas could be classified into 7 groups based on the differing expressions of Ia, Ly-1, Ly-2, Ly-4, and Ly-6 specificities; all tumors were H-2+, Ly-5+, Ly-9+, and Ly-15+. Thus a wide variety of phenotypically diverse tumors could be detected; if these represent the clonal expansion of different functional subsets, they could provide a valuable set of functional T-cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000032:analysis / Q000235:genetics; D000954:Antigens, Surface / Q000032:analysis; D002462:Cell Membrane / Q000276:immunology; D006183:H-2 Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007519:Isoantigens / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000145:classification / Q000209:etiology / Q000276:immunology; D009381:Neoplasms, Radiation-Induced / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens; D013945:Thymoma / Q000145:classification / Q000209:etiology / Q000276:immunology*; D013953:Thymus Neoplasms / Q000145:classification / Q000209:etiology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000954:Antigens, Surface; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D018800:Thy-1 Antigens