In vivo incorporation of 3H-choline in alveolar type-II pneumocytes in mice treated with butylated hydroxytoluene (BHT).
Butylated hydroxytoluene has been previously shown to damage alveolar type-I cells followed by proliferation of type-II pneumocytes which then redifferentiate into intermediate and newly formed type-I epithelial cells. In relation to this transformation process, in vivo incorporation of H3-choline, a precursor of the main component of lung surfactant, was studied in type-II cells of BHT-treated mice by electron microscopic radioautography. The labeling density was reduced in some type-II cells at 2, 3 and 5 days after a single injection of BHT. Cellular compartment analysis indicated that the transformation of type-II cells is accompanied by a notable loss of their lamellar body component as well as a decreased radioautographic labeling associated with this cell type, possible in relation with modified synthesis of phospholipid material of surfactant.
D000818:Animals; D001345:Autoradiography; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002794:Choline / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011663:Pulmonary Surfactants / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D011663:Pulmonary Surfactants; D014316:Tritium; D002084:Butylated Hydroxytoluene; D002794:Choline