Release of immunoreactive beta-endorphin from synaptosome-enriched fraction of rat hypothalami.
Immunoreactive beta-endorphin is localized mainly in the synaptosome-enriched subcellular fraction from rat hypothalami. In this report, we demonstrate that release of immunoreactive beta-endorphin from the synaptosome-enriched subcellular fraction is increased by 150% in a medium containing high potassium. This stimulatory effect was dependent on temperature and calcium ion in the medium. These results suggest that hypothalamic beta-endorphin can be released from nerve endings during membrane depolarization processes.
D000818:Animals; D004723:Endorphins / Q000378:metabolism* / Q000502:physiology; D007031:Hypothalamus / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008564:Membrane Potentials; D018377:Neurotransmitter Agents; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism; D001615:beta-Endorphin
D004723:Endorphins; D018377:Neurotransmitter Agents; D001615:beta-Endorphin; D011189:Potassium Chloride