Total poly(A+)-messenger RNA from bovine lens cofractionates with sucrose purified fiber cell plasma membrane.
Poly(A+)-messenger RNA was isolated from bovine lens as well as poly(A+)-mRNA from crude and sucrose-purified plasma membrane. Bovine lens MP26 antisera was also propagated in rabbits, from which anti-MP26 IgG was partially purified and used to assay for the specific synthesis of MP26 during in vitro translation of the isolated poly(A+)-mRNAs. We found that membrane-associated poly(A+)-mRNA supported the synthesis of proteins which were identical to those translated from total lens fiber cell poly(A+)-mRNA. These proteins included MP26, as assayed by immunoprecipitation of [35S]-labeled MP26.
D000818:Animals; D020346:Aquaporins; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis*; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*
D020346:Aquaporins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C030328:aquaporin 0; D011061:Poly A