[Comparative characteristics of the properties of erythrocyte Na,K-ATPase in man and the carp Cyprinus carpio].
Studies have been made on some of the properties of Na,K-ATPase of a nuclear erythrocytes of man and nuclear erythrocytes of the carp Cyprinus carpio. Human erythrocytes yielded the enzymic activity only after their treatment by a detergent Twin-20; under optimal conditions, it amounted to 2.6 mcmole /ml of erythrocytes per 1 h. In carp erythrocytes, Na,K-ATPase activity could be detected without detergent treatment, being 10-fold higher under optimal conditions than that in human erythrocytes. Repetitive washing of carp erythrocytes from the plasma (for more than 3 times), significantly increased their viscosity and resulted in spontaneous hemolysis. Simultaneously , the activity of Na,K-ATPase increased 2-10 times depending on the composition of incubation media. Under these conditions, the pattern of changes in the enzymatic activity, resulting from shifts in Mg2+ and EDTA concentrations, was altered. The presence of latent Na,K-ATPase activity in the erythrocytes in explained by a low permeability of membranes to ATP and ions. Exogeneous ATP cannot be utilized by the enzyme in the intact human erythrocytes, whereas intact carp erythrocytes exhibit significant permeability to the exogeneous substrate. It is suggested that in vivo this fact may be of physiological importance.
D000251:Adenosine Triphosphatases / Q000097:blood; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002347:Carps / Q000097:blood*; D002467:Cell Nucleus / Q000201:enzymology; D003530:Cyprinidae / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase
Sravnitel'naia kharakteristika svoĭstv Na,K-ATPazy éritrotsitov cheloveka i karpa Cyprinus carpio.