title: Assay of tyrosine 3-monooxygenase using the coupled nonenzymatic decarboxylation of dopa.
abstract: Dopa was found to be decarboxylated nonenzymatically by ferricyanide over the range of pH 4.5 to 10. On the basis of this finding, a new assay method of tyrosine 3-monooxygenase using the coupled nonenzymatic decarboxylation by ferricyanide was developed. The new method was excellent not only in sensitivity and reliability, but also in convenience.
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D055598:Chemical Phenomena; D002621:Chemistry; D003653:Decarboxylation; D004295:Dihydroxyphenylalanine; D005292:Ferricyanides; D006863:Hydrogen-Ion Concentration; D051381:Rats; D013696:Temperature; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
chemical_list: D005292:Ferricyanides; D002245:Carbon Dioxide; D004295:Dihydroxyphenylalanine; D014446:Tyrosine 3-Monooxygenase