title: Cryopreserved human thyroid tissue for TSAb determination: comparison with fresh tissue.
abstract: A thyroid-stimulating antibody assay using human normal thyroid slices was performed for studying whether the cryopreservation technique produced a sensitive substrate for cyclic-AMP generation. Cryopreservation was carried out in sterile 15% glycerol 0.9% NaCl placed in a hexane-nitrogen bath at -196 degrees C and stored at -80 degrees C for 1-5 weeks. Dose-response curves for untreated Graves' hyperthyroid patients IgG and bovine TSH were similar using fresh and cryopreserved slices. Assay sensibility was 5 mU/ml TSH. A high correlation (r = 0.91; p less than 0.001; n = 41) was found for percentage of cyclic-AMP increase to b-TSH and Graves' IgG comparing both methods. Studying 19 Graves' hyperthyroid patients, positive results were found in 63 and 52% for fresh and cryopreserved slices respectively. The cryopreservation technique used in the study was efficient for TSAb determinations.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D000242:Cyclic AMP / Q000096:biosynthesis; D005615:Freezing; D006111:Graves Disease / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D018828:Immunoglobulins, Thyroid-Stimulating; D013961:Thyroid Gland / Q000276:immunology* / Q000378:metabolism; D013972:Thyrotropin / Q000276:immunology; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D013972:Thyrotropin; D000242:Cyclic AMP