Radioimmunoassay of somatostatin in human plasma.
A radioimmunoassay procedure for determination of somatostatin immunoactivity in human plasma is described. Labelling of tyrosine-1-somatostatin is performed by chloramine T. Specific activity of the tracer is 1.34 mol iodine for 1 mol of somatostatin. The immunoreactivity is 85%. Antisera are produced in rabbits at a titre of 179 X 10(-12) mol somatostatin per ml antiserum. Human plasma is extracted by n-butanol/2 mol/l acetic acid. Recovery rate is 85-105%. The intra-assay CV varies from 12% to 1.7%, the inter-assay CV from 16% to 7.5%, depending on the concentration of somatostatin.
D000818:Animals; D000918:Antibody Specificity; D006651:Histocytochemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D010179:Pancreas / Q000166:cytology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D013004:Somatostatin / Q000097:blood* / Q000276:immunology
D007106:Immune Sera; D013004:Somatostatin