title: Activity of inosine triphosphate pyrophosphohydrolase in fresh and stored human erythrocytes.
abstract: The accumulation of inosine triphosphate (ITP) in human erythrocytes incubated with inosine depends on the activity of inosine triphosphate pyrophosphohydrolase (ITPH). High activity of this enzyme is accompanied by a low concentration of ITP and conversely. We showed that ITPH activity decreases with the prolongation of blood preservation time. As a consequence there is a lower accumulation of ITP in fresh erythrocytes incubated in a medium containing high concentrations of inosine, pyruvate and phosphate (IPP) than in red blood cells preserved at 4 degrees C. Synthesis of ITP in erythrocytes incubated in IPP medium being so intensive, it seems possible that during incubation an intermediate accumulates which decreases ITPH activity.
mesh_terms: D001793:Blood Preservation; D002852:Chromatography, Ion Exchange; D003080:Cold Temperature; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007288:Inosine / Q000494:pharmacology; D007290:Inosine Diphosphate / Q000096:biosynthesis; D007291:Inosine Monophosphate / Q000096:biosynthesis; D007293:Inosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D011755:Pyrophosphatases / Q000378:metabolism*; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D013997:Time Factors; D000097630:Inosine Triphosphatase
chemical_list: D007288:Inosine; D007290:Inosine Diphosphate; D007291:Inosine Monophosphate; D007293:Inosine Triphosphate; D010710:Phosphates; D011755:Pyrophosphatases; D011773:Pyruvates; D019289:Pyruvic Acid; D000097630:Inosine Triphosphatase