Interaction of polyribosomal components and polyribonucleotides with microtubule proteins.
To demonstrate the affinity of RNA-containing polyribosomal components (isolated from L5178y cells) to microtubules, microtubule protein was attached to an insoluble matrix. In contrast to ribosomes, poly(A)(+)mRNA and poly(A)-RNP were found to bind to the matrix. Using synthetic polyribonucleotides, no significant differences in the binding properties of single- and double stranded polymers of different base composition to microtubule protein were observed. However, binding is dependent on the size of the polymer; a minimal chain length of 12 nucleotide units is required.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D011131:Polyribonucleotides / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011131:Polyribonucleotides; D011506:Proteins; D012333:RNA, Messenger; D011061:Poly A