Rapid gas chromatographic measurement of diazepam and its metabolites desmethyldiazepam, oxazepam, and 3-hydroxydiazepam (temazepam) in small samples of plasma.
A rapid method was developed for the determination of diazepam, desmethyldiazepam, oxazepam, and 3-hydroxydiazepam (temazepam) in human plasma using electron capture gas chromatography. Following a single extraction with benzene from 0.2 ml plasma, diazepam and its metabolites were quantitated on a SP-2250 column with medazepam as internal standard. The method has a sensitivity limit of 4 ng diazepam, oxazepam, and desmethyl-diazepam, and 15 ng 3-hydroxydiazepam per ml of plasma.
D014151:Anti-Anxiety Agents / Q000097:blood*; D001711:Biotransformation; D002849:Chromatography, Gas / Q000379:methods; D003975:Diazepam / Q000097:blood; D006801:Humans; D003708:Nordazepam / Q000097:blood; D010076:Oxazepam / Q000097:blood; D013693:Temazepam / Q000097:blood
D014151:Anti-Anxiety Agents; D003708:Nordazepam; D010076:Oxazepam; D013693:Temazepam; D003975:Diazepam