Induction by carrot allelochemicals of insecticide-metabolising enzymes in the southern armyworm (Spodoptera eridania).
Carrot foliage monoterpenes induce cytochrome P-450 up to 2.9-fold, NADPH cytochrome c (P-450) reductase up to 1.6-fold, NADPH-oxidation up to 3.8-fold, aldrin epoxidation up to 1.5-fold in southern armyworm larval midgut tissues when incorporated in their diet at 0.2% for 3 days. Stigmasterol and ergosterol did not substantially induce microsomal oxidase activities and significantly inhibited GSH S-aryltransferase activity and sulfotransferase activity. Coumarin did not substantially affect microsomal oxidase and sulfotransferase activity but is the most potent inducer of GSH S-aryltransferase activity, increasing this activity 7-fold. None of the chemicals is acutely toxic to the sixth instar larvae or affect the larval weight gain except coumarin which significantly depressed the maximal body weight attained.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects*; D004875:Ergosterol / Q000494:pharmacology; D005982:Glutathione Transferase / Q000096:biosynthesis; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D007915:Lepidoptera / Q000187:drug effects*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D009036:Moths / Q000187:drug effects* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis; D010089:Oxidoreductases, N-Demethylating / Q000096:biosynthesis; D013265:Stigmasterol / Q000494:pharmacology; D013466:Sulfurtransferases / Q000096:biosynthesis; D013729:Terpenes / Q000494:pharmacology*; D014675:Vegetables
D003374:Coumarins; D007306:Insecticides; D013729:Terpenes; D003577:Cytochrome P-450 Enzyme System; D013265:Stigmasterol; C030123:coumarin; D006899:Mixed Function Oxygenases; C019647:aldrin epoxidase; C030154:4-chloro-N-methylaniline demethylase; D010089:Oxidoreductases, N-Demethylating; D009251:NADPH-Ferrihemoprotein Reductase; D005982:Glutathione Transferase; D013466:Sulfurtransferases; D004875:Ergosterol