Expression of Thy-1, H-2, and NS-4 cell surface antigens and tetanus toxin receptors in early postnatal and adult mouse cerebellum.
The expression of several cell surface components (Thy-1, H-2 and NS-4 antigens and tetanus toxin receptors) was studied by indirect immunofluorescence in situ using histological sections and in vitro using freshly dissociated and cultured cells from mouse cerebellum. Thy-1 alloantigen is expressed in adult cerebellum predominantly in neuron-rich regions, i.e. molecular, Purkinje cell, and granular layers, however, it is not detectable at postnatal day 8. In cerebellar cultures of 6-day-old mice Thy-1 is absent from more than 99% of all cells when these are maintained as monolayers in vitro for up to 3 days. After 4 days in vitro some GFA protein-positive astrocytes and some fibronectin-positive fibroblast-like cells start to express Thy-1 antigen. After 14 days in vitro not all fibroblast-like cells and astrocytes are Thy-1 antigen-positive. Neurons with small cell bodies and oligodendrocytes never express Thy-1 at any stage examined. H-2 is not expressed sufficiently to be detectable in histological sections in early postnatal or adult cerebellum. In cerebellar cultures of 6-day-old mice H-2 becomes detectable on some fibroblast-like cells and some astrocytes after 7 days in culture. In histological sections of adult and early postnatal cerebellum NS-4 antigen and tetanus toxin receptors are expressed at higher levels on more mature granule cells. In cerebellar cultures NS-4 antigen and tetanus toxin receptors are expressed on neurons. Occasionally some astroglia can also show detectable levels of expression. NS-4 antigen is also present on some 04 antigen-positive oligodendrocytes, while tetanus toxin receptors are never detectable on these cells.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D001253:Astrocytes / Q000276:immunology; D002478:Cells, Cultured; D002531:Cerebellum / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000032:analysis*; D008565:Membrane Proteins; D051379:Mice; D009474:Neurons / Q000276:immunology; D009836:Oligodendroglia / Q000276:immunology; D011950:Receptors, Cholinergic / Q000032:analysis*; D013744:Tetanus Toxin; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D006183:H-2 Antigens; D008565:Membrane Proteins; D011950:Receptors, Cholinergic; D013744:Tetanus Toxin; D018800:Thy-1 Antigens; C028728:tetanus toxin receptor