[15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants.
Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.
D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005971:Glutamates / Q000096:biosynthesis*; D018698:Glutamic Acid; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D051379:Mice; D009587:Nitrogen Isotopes; D009924:Organ Culture Techniques
D000596:Amino Acids; D005971:Glutamates; D009587:Nitrogen Isotopes; D018698:Glutamic Acid; D007930:Leucine