title: Comparative biochemical properties of normal and activated human ras p21 protein.
abstract: Human Ha-ras1 cDNAs encoding normal and activated p21 polypeptides have been efficiently expressed in Escherichia coli and the biochemical activities associated with each polypeptide compared. In addition to the guanine nucleotide binding activity, normal p21 displays a GTPase activity which is selectively impaired by a mutation which activates its oncogenic potential.
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000235:genetics; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D015689:Oncogene Protein p21(ras); D009857:Oncogenes; D010766:Phosphorylation; D011485:Protein Binding; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; D006160:Guanosine Triphosphate; D004247:DNA; D004262:DNA Restriction Enzymes; D020558:GTP Phosphohydrolases; C501009:HRAS protein, human; D015689:Oncogene Protein p21(ras); D016283:Proto-Oncogene Proteins p21(ras)