Immunostaining of dog pituitary somatotrophs with anti-somatostatin serum: identification of receptors for somatostatin.
An antiserum to cyclic somatostatin was applied to tissues of the dog anterior pituitary gland in association with the immunohistochemical procedure. A positive immunoreaction was observed in cells identified as somatotrophs. Since the latter staining was not abolished by preadsorbtion of the anti-somatostatin serum with excess antigen, it is proposed that the positive stain represents pituitary receptor sites for the inhibiting factor. Using a similar protocol, receptors in the anterior pituitary of the rat could not be visualized even after preincubation with somatostatin.
D000818:Animals; D004285:Dogs; D007124:Immunoenzyme Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism*
D011956:Receptors, Cell Surface; D017481:Receptors, Somatostatin; D013004:Somatostatin