Evidence for the presence of somatostatin 28 in plasma.
Somatostatin-like immunoreactivity (SLI) from dog and rat plasma eluted from Biogel P-6 columns as three distinct peaks. A large-molecular-weight peak was present in the void volume of the column, an intermediate-sized peak (SLI28) coeluted with synthetic somatostatin 28 (S-28), and a small-molecular-weight peak (SLI14) coeluted with SRIF. Material from the SLI28 peak diluted in parallel to the S-28 standard in the radioimmunoassay and behaved identically to S-28 on high pressure liquid chromatography (HPLC). Levels of SLI28 in the portal vein were consistently greater than the simultaneously measured peripheral levels (portal peripheral ratio 2.2 +/- 0.2). Venous samples drawn from multiple sites suggested that SLI28 is segregated by the duodenum and/or pancreas and the intestine. This data is consistent with the possibility that S-28 is a hormone distinct from SRIF.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D008970:Molecular Weight; D010455:Peptides / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D013004:Somatostatin / Q000097:blood*; D053561:Somatostatin-28
D010455:Peptides; C029993:somatostatin-like peptides; D013004:Somatostatin; D053561:Somatostatin-28