Enzymatic determination of several D-amino acids using luminol-mediated chemiluminescence.
A method for the quantitative determination of several D-amino acids in the range of 0.05-1 nmol per assay (0.25-5 microM) is described. It is insensitive to the presence of excesses of the respective L-amino acids. The assay system employs D-amino-acid oxidase (hog kidney), peroxidase (horse radish) and luminol; the total photon output elicited by the oxidation of the D-amino acids is determined. The different reactivity of individual D-amino acids with D-amino-acid oxidase limits the applicability of the assay. Indications for the usefulness of immobilized enzymes in D-amino-acid analysers are also given.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D013237:Stereoisomerism; D013552:Swine
D000596:Amino Acids; D008165:Luminol; D006735:Horseradish Peroxidase; D003605:D-Amino-Acid Oxidase