Identification of lactate dehydrogenase-X translated in vitro from mouse testicular poly A-containing mRNA.
1. The LDH-X polypeptide was specifically immunoprecipitated from the cell-free translation products of poly A-containing mRNA from mouse testes, and it represents 1-2% of the total proteins synethesized in vitro. 2. The in-vitro synthesized LDH-X polypeptide appears to have the same mol. wt of 36,000 as mouse authentic LDH-X and, thus, any presequence of LDH-X must be very short, if present at all. 3. The LDH-X was not found in the mouse liver mRNA translation products immunoprecipitated by anti-LDH-X antibodies.
D000818:Animals; D007120:Immunochemistry; D066298:In Vitro Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D011061:Poly A / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D007770:L-Lactate Dehydrogenase