Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
The MRC OX-2 monoclonal antibody recognises membrane glycoproteins of Mr 41,000 in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle, and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was present throughout the cerebellum, with staining of both grey and white matter. Blood vessels were also stained. The Purkinje cell layer appeared to be unlabelled. Double-immunofluorescence staining of cerebellar interneurone cultures with MRC OX-2 antibody and tetanus toxin showed that all tetanus-positive cells (neurones) were MRC OX-2-positive. Glial fibrillary acidic protein-positive astrocytes were not labelled by MRC OX-2 antibody. Thus OX-2 antigen is one of the few biochemically characterised components of neuronal membranes and its properties are compared with those of the neuronal membrane glycoprotein Thy-1.
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface / Q000032:analysis*; D001253:Astrocytes / Q000276:immunology; D002478:Cells, Cultured; D002531:Cerebellum / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D009474:Neurons / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D006023:Glycoproteins; D008565:Membrane Proteins; D018800:Thy-1 Antigens; C042739:antigens, CD200