Amino acid content of guinea pig perilymph collected under conditions of quiet or sound stimulation.
With the cochlear aqueducts blocked, guinea pig cochleas were perfused with artificial perilymph. The collected perfusates were treated with 5-(dimethylamino)-1-naphthalenesulfonyl chloride (dansyl-Cl). The derivatized constituents were analyzed by high- performance liquid chromatography using fluorometric detection. The concentration of none of the compounds measured, including the putative transmitters glutamate and aspartate, was found to increase in the perfusate in response to sound stimulation. These results do not support the suggestion that one of these compounds might be primary afferent transmitter of audition.
D000161:Acoustic Stimulation; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003051:Cochlea / Q000502:physiology*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000502:physiology; D006309:Hearing; D007761:Labyrinthine Fluids / Q000032:analysis*; D018377:Neurotransmitter Agents / Q000502:physiology*; D010498:Perilymph / Q000032:analysis*
D000596:Amino Acids; D018377:Neurotransmitter Agents