Immunoreactive peptides related to dynorphin B (= rimorphin) in the rat brain.
We have developed a radioimmunoassay for synthetic dynorphin B, a novel opioid tridecapeptide, which shares a common precursor molecule with dynorphin1-17 (= dynorphin A) and the neo-endorphins. The levels of immunoreactivity towards this peptide in rat brain and pituitary show a pattern quantitatively and qualitatively similar to those found for dynorphin A and alpha-neo-endorphin in earlier studies. The antiserum used was highly specific with only dynorphin-32 and dynorphin B-29, both of which contain the dynorphin B sequence, showing substantial cross-reactivity. Gel filtration of whole rat brain extracts in combination with HPLC analysis provide strong evidence for the existence of these latter two peptides in rat brain.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis*; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives* / Q000032:analysis; D008297:Male; D010446:Peptide Fragments / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D004723:Endorphins; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; D004399:Dynorphins; C036200:rimorphin