Characterization of beta-adrenergic receptors in rat ovary.
Specific beta-adrenergic receptors were characterized in rat ovary, using an in vitro receptor binding assay with [3H]dihydroalprenolol. The ligand was bound to a single class of saturable and high affinity receptors (dissociation constant = 3.5 nM and maximum capacity = 3.4 pmol/g tissue). In competition studies, the binding was stereoselective for the (-)-isomer of isoproterenol. Known beta-adrenergic agonists displaced the ligand from ovarian receptors with the following order of potency: isoproterenol greater than epinephrine much greater than norepinephrine, which is typical for a beta 2-adrenergic receptor.
D000318:Adrenergic beta-Agonists / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000378:metabolism*; D000526:Alprenolol / Q000031:analogs & derivatives*; D000818:Animals; D001667:Binding, Competitive; D004082:Dihydroalprenolol / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D010053:Ovary / Q000378:metabolism*; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013237:Stereoisomerism; D014316:Tritium
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D011943:Receptors, Adrenergic, beta; D014316:Tritium; D004082:Dihydroalprenolol; D000526:Alprenolol