The production of carcinomas in vivo by the intravenous or subcutaneous injection of a hepatocellular tumour cell line.
Suspensions of cells of an aflatoxin-induced hepatocellular carcinoma cell line, which grow rapidly as tumours when injected subcutaneously into nude mice, have been injected subcutaneously and also intravenously via the hepatic portal or tail vein into syngeneic host animals. Efficiency of the injections was checked histologically and histochemically in parallel experimental animals. Subcutaneous injections consistently resulted in the appearance of solid tumours at the site of injection within 1 month. However, despite the apparent importance of the establishment of a blood supply when tumours are grown subcutaneously, only a low incidence of tumours (less than 20%) was detected in animals receiving intravenous injections. These results suggest that the proposed use of injection of liver cell suspensions into the portal tract as a sensitive means of detecting transformation may only be of limited value.
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D005260:Female; D006651:Histocytochemistry; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D011503:Transglutaminases
D000217:Acyltransferases; D011503:Transglutaminases