Assay of tyrosine 3-monooxygenase using the coupled nonenzymatic decarboxylation of dopa.
Dopa was found to be decarboxylated nonenzymatically by ferricyanide over the range of pH 4.5 to 10. On the basis of this finding, a new assay method of tyrosine 3-monooxygenase using the coupled nonenzymatic decarboxylation by ferricyanide was developed. The new method was excellent not only in sensitivity and reliability, but also in convenience.
D000818:Animals; D002245:Carbon Dioxide; D055598:Chemical Phenomena; D002621:Chemistry; D003653:Decarboxylation; D004295:Dihydroxyphenylalanine; D005292:Ferricyanides; D006863:Hydrogen-Ion Concentration; D051381:Rats; D013696:Temperature; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D005292:Ferricyanides; D002245:Carbon Dioxide; D004295:Dihydroxyphenylalanine; D014446:Tyrosine 3-Monooxygenase