title: Two types of pyriform Ab amacrine cells in the goldfish retina: an EM analysis of [3H]GABA uptake and somatostatin-like immunoreactivity.
abstract: Pyriform Ab amacrine cells in the goldfish retina take up [3H]GABA and show somatostatin-like immunoreactivity (SLIR), leading to the question of whether these two markers are labeling the same or different types of Ab amacrine cells. We used a double-label radio/immuno-technique at the electron microscopical level to visualize the comparative location of [3H]GABA uptake and SLIR in Ab amacrine cells and their processes. SLIR was restricted to large dense-cored vesicles in processes of Ab amacrine cells. In no case were processes labeled with SLIR observed to take up [3H]GABA. Thus, there are at least two types of pyriform Ab amacrine cells: one that takes up [3H]GABA and one that shows SLIR. These may correspond to the two types of Ab amacrine cells described by Cajal in his classic studies on the retina.
mesh_terms: D000818:Animals; D001345:Autoradiography; D003530:Cyprinidae / Q000033:anatomy & histology*; D006054:Goldfish / Q000033:anatomy & histology*; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D013004:Somatostatin; D005680:gamma-Aminobutyric Acid