title: Immunohistochemical studies of intrahypothalamic somatostatin-containing neurons in rat.
abstract: Intrahypothalamic somatostatin-containing neurons were investigated immunohistochemically. In intact rats, immunoreactive cell bodies appeared in the rostral periventricular area, and immunoreactive beaded fibers were observed to terminate in the median eminence and to form delicate networks surrounding immunonegative cell bodies within the medial preoptic, suprachiasmatic, arcuate, ventromedial and premammillary nuclei. Intraventricular colchicine infusion resulted in the appearance of immunoreactive cell bodies in the arcuate, ventromedial and suprachiasmatic nuclei, and an increase in the number of cell bodies seen in the periventricular area. Complete deafferentation of the medial-basal hypothalamus excluding the rostral periventricular area caused the immunoreactive structures in the median eminence to disappear and enhanced the staining of periventricular cell bodies. In the arcuate and ventromedial nuclei, the immunoreactive fiber networks were left intact and the immunoreactive cell bodies were occasionally recognized. Horizontal knife cut between the arcuate nuclei and median eminence did not alter immunoreactivity in either region. Neonatal administration of MSG caused only the disappearance of arcuate nuclei. The results indicate that two kinds of somatostatin neuronal systems exist in rat hypothalamus: one is involved in the production of hormonal somatostatin and the other serves for the regulation of neuronal activities in restricted hypothalamic nuclei.
mesh_terms: D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012970:Sodium Glutamate / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D013004:Somatostatin; D003078:Colchicine; D012970:Sodium Glutamate