A clinically useful DNA probe closely linked to haemophilia A.
Genetic linkage studies were done in families segregating for haemophilia A with an X-chromosome-specific probe, DX13, which has been localised to band Xq28. When DNA is digested with the restriction enzyme Bg1 II, this probe recognises a restriction fragment length polymorphism, for which 50% of females are heterozygous. No crossovers were seen between the haemophilia A locus and the DX13 locus (lod score = 5.4 at recombination fraction [theta] 0.0; 95% confidence limits of theta, 0-12%). This indicates that the DX13 probe is closely linked to the haemophilia A locus and is likely to prove useful in carrier detection and prenatal diagnosis.
D000483:Alleles; D001426:Bacterial Proteins; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006580:Genetic Carrier Screening; D008040:Genetic Linkage; D006467:Hemophilia A / Q000175:diagnosis* / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011296:Prenatal Diagnosis; D014960:X Chromosome
D001426:Bacterial Proteins; D004247:DNA; D004262:DNA Restriction Enzymes; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific