2-amino-4-phosphonobutyric acid and N-methyl-D-aspartate differentiate between "3H]glutamate and [3H] aspartate binding sites in bovine retina.
Three pharmacologically and kinetically distinct binding sites for acidic amino acids were observed in synaptic membranes from bovine retina. One site preferentially binds aspartate with an apparent Kd of 6.3 nM and Bmax of 0.033 pmol/mg protein. [3H] Glutamate binds preferentially to two sites which have KdS of approximately 10 and 800 nM; and BmaxS of 0.148 and 0.417 pmol/mg protein, respectively. Only one of these sites, the 800 nM glutamate site, displayed a pharmacological specificity which was in extensive agreement with that reported for the putative glutamate receptor previously localized to a specific set of retinal neurons, the on-center, depolarizing bipolar cells.
D000613:Aminobutyrates / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006710:Homocysteine / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques; D016202:N-Methylaspartate; D009474:Neurons / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism
D000613:Aminobutyrates; D005971:Glutamates; D006710:Homocysteine; C007956:homocysteic acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; C012729:2-amino-4-phosphonobutyric acid