A study of androgen-stimulated L-leucine transport by the intestine of rainbow trout (Salmo gairdneri Richardson) in vitro.
The intestinal transport of L-leucine and fluid was studied in response to in vitro administration of 17 alpha-methyltestosterone (MT; 50 micrograms/ml) and 11-ketotestosterone (KT; 10 micrograms/ml), using strips of rainbow trout intestine incubated for 60 min in vitro. Both MT and KT increased the intestinal absorption of the amino acid without significantly affecting fluid transport. Addition of ouabain, 2,4-dinitrophenol (DNP) and sodium fluoride (NaF) reduced the intestinal transport of leucine. It would appear that ouabain and DNP significantly impair the action of MT, although their effect on KT is uncertain. It is suggested that the steroid-induced leucine transport during the latter 40 min of incubation might be, in part, the result of an increased activity of Na+,K+-ATPase.
D019297:2,4-Dinitrophenol; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D004140:Dinitrophenols / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007930:Leucine / Q000378:metabolism*; D008777:Methyltestosterone / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology; D012487:Salmonidae / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors; D014337:Trout / Q000378:metabolism*
D004140:Dinitrophenols; D013739:Testosterone; D010042:Ouabain; D012969:Sodium Fluoride; D000254:Sodium-Potassium-Exchanging ATPase; D007930:Leucine; C003600:11-ketotestosterone; D019297:2,4-Dinitrophenol; D008777:Methyltestosterone