Properties of clathrin coat structures.
Clathrin polymerizes to form characteristic coat structures (baskets) closely resembling those found on coated vesicles. Two sizes of baskets are formed from clathrin, depending on the purity of the preparation and on other factors. A protein of Mr 110 000 has been separated from clathrin by lysine-Sepharose chromatography which is needed for the formation of 150S baskets. In its absence, polymerization results in the larger size baskets, i.e., 300S. Addition of Ca2+ or Mg2+ stimulates 300S formation in the presence of the 110 000 protein.
D002118:Calcium / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002966:Clathrin; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure*; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D011992:Endosomes / Q000648:ultrastructure*; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000302:isolation & purification; D008854:Microscopy, Electron; D014461:Ultracentrifugation
D002966:Clathrin; D046911:Macromolecular Substances; D008565:Membrane Proteins; D002118:Calcium