title: High affinity uptake and Ca2+-dependent release of glutamic acid in the developing cerebellum.
abstract: This study was undertaken in order to evaluate the postulated role of glutamic acid as the neurotransmitter for the parallel fibers of the cerebellar cortex. We studied the Ca2+-dependent release and the high affinity uptake of glutamic acid in the developing cerebellum. The Ca2+-dependent release of glutamic acid from cerebellar molecular layer during development closely follows the time course of parallel fibers synaptogenesis. Little glutamic acid release was observed at 15 days, then it increased to the adult values at the 21st postnatal day. In the rat the bulk of synapses of the parallel fibers appear between the 15th and the 21st postnatal days, the time at which the nerve terminals of the climbing fibers, the other excitatory input to the Purkinje cells, are already developed. An enhanced Na+-dependent, high affinity uptake of glutamic acid was observed in the developing cerebellum relative to the adult rat. That this higher accumulation of glutamic acid is not related to a releasable pool is suggested by the fact that an enhanced glutamic acid, Ca2+-dependent release relative to the adult was not observed. These results support the view that glutamic acid is the transmitter for the cerebellar parallel fibers.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008564:Membrane Potentials; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000502:physiology; D009435:Synaptic Transmission; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D005971:Glutamates; D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D002118:Calcium