Distribution and some properties of the glutathione S-transferase and gamma-glutamyl transpeptidase activities of rainbow trout.
1. Gills, kidney, intestinal caeca and liver of trout have glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene (200 500 nmol/min/mg protein), and reduced glutathione (0.5 2.0 mmol/kg tissue). 2. Only kidney and intestinal caeca have substantial gamma-glutamyl transpeptidase activity with gamma-glutamyl-rho-nitroanilide (2-9 nmol/min/mg protein). 3. Renal gamma-glutamyl transpeptidase is membrane-bound and has similar kinetic properties to its mammalian counterparts. 4. The data are consistent with the presence of a mercapturic acid pathway in trout.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002432:Cecum / Q000201:enzymology; D005880:Gills / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D012487:Salmonidae / Q000378:metabolism*; D014018:Tissue Distribution; D011503:Transglutaminases; D014337:Trout / Q000378:metabolism*
D000217:Acyltransferases; D011503:Transglutaminases; D005982:Glutathione Transferase