[Changes in the adenosinetriphosphatase activity in plasma membranes incubated in vitro in the presence of 4-hydroxy-2,3-nonenal].
Cation-dependent ATPase activities of rat liver plasmamembranes incubated "in vitro" with 4-hidroxy-2,3-nonenal (HNE, an aldehyde from peroxidative decomposition of biological membrane lipid moieties) are investigated. Mg++-ATPase activity is inhibited significantly by all the doses of HNE used (13,9, 4,1,1,2, 0,35 and 0,10 microM). Evidences for the inhibition of Mg++- Na+- K+- ATPase activity are also presented. Ca++- ATPase activity is strongly increased when rat liver plasmamembranes are incubated in presence of HNE 13,9 microM. Our results suggest that HNE may play a role in the control of intracellular cation levels acting directly on mechanisms of plasmamembranes ion transport.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000447:Aldehydes; D000251:Adenosine Triphosphatases; C030704:magnesium sodium potassium ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; C027576:4-hydroxy-2-nonenal
Variazioni delle attivita' adenosintrifosfatasiche in plasma membrane incubate "in vitro" in presenza di 4-idrossi-2,3-nonenale.