title: Cell mediated immune response of Bufo arenarum and Leptodactylus ocellatus (Amphibia, Anura) to mycobacterial antigens.
abstract: The lymphocyte transformation test was applied to the study of cell mediated immune (CMI) response in vitro to mycobacterial antigens in Bufo arenarum and Leptodactylus ocellatus previously inoculated with a suspension of Mycobacterium tuberculosis. The stimulated samples of blood both in B. arenarum and L. ocellatus showed a significant increase in the percent of blastic dedifferentiation (almost 100% with respect to controls) suggesting the presence of a positive CMI response to PPD. The differences were observed on the fifth day of incubation and lasted until the seventh day.
mesh_terms: D000663:Amphibians / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002022:Bufo arenarum / Q000276:immunology*; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial