Immunological studies of gamma-glutamyltransferases from various animal organs using a sensitive electrophoretic method.
A simple and sensitive method of studies on interactions of antibodies with the light forms of gamma-glutamyltransferases was elaborated using polyacrylamide gel electrophoresis and the fluorescent technique for localization of the enzyme. Rabbit antibodies against bovine and rat kidney enzymes were used. gamma-Glutamyltransferases from various organs of the same animal were immunologically indistinguishable, but distinct immunological differences were observed between the enzymes from various animal species. The new method of studies on immunological reaction between antibody and the enzymes appeared more sensitive than the double immunodiffusion method.
D000818:Animals; D002417:Cattle; D002645:Chickens; D006224:Cricetinae; D003429:Cross Reactions; D004586:Electrophoresis; D006168:Guinea Pigs; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D012756:Sheep; D013154:Spleen / Q000201:enzymology; D013552:Swine; D005723:gamma-Glutamyltransferase / Q000276:immunology*
D005723:gamma-Glutamyltransferase