Effects of insulin, dexamethasone and phorbol ester on virus production in cultivated DBA/2 mouse leukemia (MLA) cells.
The in vitro production of intracytoplasmic oncovirus A-particles and of atypical mammary tumor virus (MLAV) from a line of DBA/2 mouse leukemia (MLA) cells were compared in the presence of insulin, dexamethasone and 12-0-tetradecanoyl-phorbol-13-acetate (TPA), which are known to stimulate the synthesis of mouse mammary tumor virus. High amounts of A-particles were recovered from cells cultivated in the presence of dexamethasone (1 microgram/ml) as compared to untreated controls; in contrast, the production of MLAV was suppressed. Insulin and TPA did not show any significant effects.
D000818:Animals; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000254:growth & development*; D051379:Mice; D008811:Mice, Inbred DBA; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects*
D007328:Insulin; D010704:Phorbols; D003907:Dexamethasone; D013755:Tetradecanoylphorbol Acetate