Presence of somatostatin-28-(1-12) in hypothalamus and pancreas.
Acid extracts from rat pancreas and hypothalamus were analyzed for the presence of the antigenic determinant corresponding to the NH2 terminus of somatostatin-28 (SS28), using an antiserum directed against amino acids 1 to less than or equal to 11 of the SS28 molecule. On gel permeation chromatography the majority of the immunoreactive material from each tissue extract eluted in one zone compatible with a peptide of 1250 daltons. Purification of this immunoreactive material by reverse-phase HPLC and cation-exchange chromatography yielded two immunoreactive peptides from each tissue extract. The amino acid compositions of both peptides in pancreas and hypothalamus correspond to the fragment 1-12 of SS28. The more hydrophobic peptide from each tissue coeluted with synthetic SS28-(1-12) on HPLC, while the other one coeluted with synthetic SS28-(1-12)-amide. We conclude that the prosomatostatin fragment Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-OH is present in both rat hypothalamus and rat pancreas.
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D007031:Hypothalamus / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000276:immunology / Q000378:metabolism*; D053561:Somatostatin-28
D010446:Peptide Fragments; D013004:Somatostatin; D053561:Somatostatin-28