Three cell lines from a spontaneous murine astrocytoma show variation in astrocytic differentiation.
Three cell lines originally derived from a spontaneous, transplantable, murine astrocytoma have been maintained in vitro. Immunofluorescence confirmed their astrocytic nature while electron microscopy revealed differences in the number and ratio of 10 nm filaments and 24 nm microtubules in the 3 lines; a feature related to the degree of astrocytic differentiation.
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D001254:Astrocytoma / Q000648:ultrastructure*; D001932:Brain Neoplasms / Q000648:ultrastructure*; D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000648:ultrastructure
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D005974:Glutamate-Ammonia Ligase