title: Effects of anaerobiosis and nitrate on the expression of succinate dehydrogenase and enzymes associated with nitrogen metabolism in Klebsiella pneumoniae.
abstract: We have shown that the low histidase activity found in anaerobic, nitrogen-limited cultures of Klebsiella pneumoniae is due to repression of the right-hand hut operon. In addition, we have examined the effects of NO3- on the aerobic and anaerobic expression of catabolite- and NH4+-repressible enzymes in this organism. NO3- permitted anaerobic growth of K. pneumoniae in minimal medium containing histidine as the sole carbon source, and histidase and succinate dehydrogenase were derepressed during anaerobic growth in histidine/NO3- medium. Use of sucrose rather than histidine as the carbon source reversed the effects of NO3- and repressed histidase and succinate dehydrogenase activities. Anaerobic growth in sucrose/NO3- medium also uncoupled the expression of urease and glutamine synthetase.
mesh_terms: D000693:Anaerobiosis; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006638:Histidine Ammonia-Lyase / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D014510:Urease / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D009566:Nitrates; D013395:Sucrose; D013385:Succinate Dehydrogenase; D001616:beta-Galactosidase; D014510:Urease; D006638:Histidine Ammonia-Lyase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen