title: Synthesis of delta-aminolaevulinate synthase in vitro using hepatic mRNA from chick embryos with induced porphyria.
abstract: Polyadenylated mRNA was isolated from chick embryo liver following induction of hepatic porphyria. The RNA was translated in vitro using a wheat germ cell-free system and delta-aminolaevulinate synthase was identified in the translation products by indirect immunoprecipitation. The enzyme was not apparent in the translation products of polyadenylated RNA from non-induced livers. The molecular weight of delta-aminolaevulinate synthase synthesized in vitro was 70000 and the protein was estimated to represent up to 5% of total products synthesised in vitro. These data demonstrate for the first time that induction of chick embryo liver delta-aminolaevulinate synthase activity in hepatic porphyria correlates with a large increase in the translational capacity of isolated polyadenylated RNA for this enzyme and, together with preliminary cDNA . RNA hybridization studies, indicate that an increase in the level of delta-aminolaevulinic synthase mRNA is responsible.
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002474:Cell-Free System; D056486:Chemical and Drug Induced Liver Injury; D002642:Chick Embryo; D004247:DNA; D008099:Liver / Q000201:enzymology*; D008107:Liver Diseases / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification / Q000502:physiology*; D011164:Porphyrias / Q000139:chemically induced / Q000201:enzymology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D000624:5-Aminolevulinate Synthetase