Role of intestinal brush border membrane aminopeptidase N in dipeptide transport.
The kinetics of uptake of radioactive label from [U-14C]Gly, L-[4,5-3H]Leu and the dipeptide [14C]Gly-L-[4,5-3H]Leu by the brush border membrane vesicles of porcine small intestine have been studied. The effect of aminopeptidase N inhibitors and leucine-binding protein on accumulation rates has also been tested. Comparison of the kinetic parameters for uptake and hydrolysis of Gly-L-Leu makes it possible to conclude that the dipeptide transfer includes two conjugated steps, viz., hydrolysis catalysed by aminopeptidase N and transport of the resultant free amino acids by a specific carrier.
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D018826:CD13 Antigens; D004151:Dipeptides / Q000378:metabolism*; D005998:Glycine / Q000494:pharmacology; D007421:Intestine, Small / Q000648:ultrastructure*; D007700:Kinetics; D007930:Leucine / Q000494:pharmacology; D008871:Microvilli / Q000201:enzymology; D008954:Models, Biological; D013552:Swine
D004151:Dipeptides; D000626:Aminopeptidases; D018826:CD13 Antigens; D007930:Leucine; D005998:Glycine