Applications of high-voltage paper electrophoresis for the characterization of drug metabolites.
1. The application of high-voltage paper electrophoresis (h.v.p.e.) to the identification of drug metabolites in urine and bile has been investigated. 2. The major urinary metabolite of [3H]salbutamol in man had an electrophoretic mobility indicative of a sulphate ester. 3. A metabolite of [14C]ranitidine present in rat bile was shown to contain an ionized group with a pKa corresponding to a carboxylic acid. 4. The electrophoretic mobility-pH profile of a metabolite of radiolabelled N"-cyano-N-[2-[5-(dimethylaminomethyl)-2-furanylmethylthio]ethyl]-N-methylguanidine (14C-AH 18801) excreted in dog urine suggested that oxidation of the tertiary amine group of the compound had occurred. 5. H.v.p.e. provided valuable information on the structure of both phases I and phase II metabolites at a stage when the material was insufficiently pure for identification by other techniques.
D000420:Albuterol / Q000652:urine; D000818:Animals; D000897:Anti-Ulcer Agents / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D002250:Carbon Radioisotopes; D004285:Dogs; D004590:Electrophoresis, Paper / Q000379:methods; D005663:Furans / Q000378:metabolism; D006635:Histamine H2 Antagonists / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011899:Ranitidine / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains
D000897:Anti-Ulcer Agents; D002250:Carbon Radioisotopes; D005663:Furans; D006635:Histamine H2 Antagonists; D004364:Pharmaceutical Preparations; C029440:AH 18801; D011899:Ranitidine; D000420:Albuterol