Multiple endorphins in neuronal hybrid cell lines.
Neuroblastoma x glioma hybrid cells (NG 108CC15) and tumors derived thereof were examined for dynorphin- and alpha-neoendorphin-like material. The techniques employed for analyses of opiate-like material were the isolated mouse vas deferens bioassay and gel chromatography and high pressure liquid chromatography in combination with radioimmunoassays. Dynorphin- and alpha-neoendorphin-like material was detected in both the hybrid cells and the corresponding tumors. Immunoreactive dynorphin and alpha-neoendorphin was also in NCB 20 hybrid cells and in tumors thereof assayed. In all samples investigated, the amounts of alpha-neoendorphin-like material was higher than that of dynorphin-like material. The results revealed considerable variability in the amount of dynorphin- and alpha-neoendorphin-activity between particular samples, suggesting the need for studies into the responsible mechanisms.
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis* / Q000494:pharmacology; D005910:Glioma / Q000032:analysis*; D006822:Hybrid Cells / Q000032:analysis*; D008297:Male; D051379:Mice; D009447:Neuroblastoma / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D014649:Vas Deferens / Q000187:drug effects; D018822:alpha-Endorphin
D004723:Endorphins; D018822:alpha-Endorphin; D004399:Dynorphins