Role of ouabain and diuretics on sodium, potassium and chloride retention in perfused rat kidney.
A novel in situ kidney perfusion technique is described in Sprague-Dawley rats. The procedure involves retrograde perfusion from the renal veins via the kidneys, and then through the renal arteries and dorsal aorta. Ouabain (15 mM) in perfusate increased Na retention by 92%, decreased K retention by 53% and produced no effect on Cl retention. Ethacrynic acid (1 mM) in perfusate decreased Na retention by 52%, increased K retention by 105% and decreased Cl retention by 27%. Furosemide (1.5 mM) in perfusate decreased Na retention by 52%, increased K retention by 47% and decreased Cl retention by 56%. The Na-K-ATPase pump localized at the peritubular side of the proximal tubule cell is ouabain sensitive and Mg dependent. An Na-K pump responsible for Na influx and K effux exists at the luminal side of the proximal tubule cell and is ethacrynic acid and furosemide sensitive.
D000818:Animals; D002712:Chlorides / Q000378:metabolism; D004232:Diuretics / Q000494:pharmacology*; D004573:Electrolytes / Q000378:metabolism*; D004976:Ethacrynic Acid / Q000494:pharmacology; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology*; D010477:Perfusion; D011188:Potassium / Q000378:metabolism / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
D002712:Chlorides; D004232:Diuretics; D004573:Electrolytes; D010042:Ouabain; D005665:Furosemide; D012964:Sodium; D008274:Magnesium; D004976:Ethacrynic Acid; D011188:Potassium