Non-steroidal anti-inflammatory agent inhibit the synthesis of IgM rheumatoid factor in vitro.
The effects of three non-steroidal anti-inflammatory agents, indomethacin, carprofen, and piroxicam, on in-vitro IgM rheumatoid-factor (IgM-RF) production by peripheral-blood mononuclear cells were studied in lymphocytes isolated from the peripheral (age 20-40 years) and elderly (age 70-80 years) volunteers selected for their known in-vitro IgM-RF production. All three drugs in therapeutic concentrations inhibited IgM-RF production approximately 50%. Addition of low doses of prostaglandin E2 (PGE2) (30 nmol/l) to the cultures containing indomethacin restored the IgM-RF production to the original levels. Neither indomethacin nor PGE2 had any effect on IgM-RF production if the T cells were irradiated before culture. Endogenous PGE2 seems to stimulate IgM-RF production through an effect on a radiosensitive T-cell population. Non-steroidal anti-inflammatory agents block PGE2 production and therefore reduce IgM-RF production in vitro. These results suggest a new mechanism for the beneficial effect of prostaglandin synthetase inhibitors in the treatment of rheumatoid arthritis.
D000328:Adult; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000188:drug therapy; D002227:Carbazoles / Q000494:pharmacology; D002478:Cells, Cultured; D003864:Depression, Chemical; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007213:Indomethacin / Q000494:pharmacology; D010894:Piroxicam; D011458:Prostaglandins E / Q000037:antagonists & inhibitors; D011725:Pyridines / Q000494:pharmacology; D012217:Rheumatoid Factor / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000528:radiation effects; D013843:Thiazines / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D002227:Carbazoles; D007075:Immunoglobulin M; D011458:Prostaglandins E; D011725:Pyridines; D013843:Thiazines; D010894:Piroxicam; D012217:Rheumatoid Factor; C007005:carprofen; D007213:Indomethacin