Inhibition of gastric H+ secretion, K+-ATPase and K+-phosphatase by estradiol in vivo and in vitro.
The effect of estrogen on the gastric acid secretion and H+-transporting enzymes, K+-ATPase and K+-phosphatase, were investigated in the rat. The maximum H+ secretory rate in response to 1 mM histamine was significantly reduced (P less than 0.05) in both the isolated gastric mucosa obtained from the rats treated with estradiol in vivo for 7 days and the mucosa directly incubated in vitro with estradiol. The inhibitory effect on the gastric enzyme activities in vitro showed a dose-dependent pattern of a noncompetitive type. The result suggested that estradiol may have a direct action on the gastric H+ secretion by inhibiting the H+ transport enzyme activities.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D027682:Cation Transport Proteins; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains
D027682:Cation Transport Proteins; D004958:Estradiol; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; C022761:potassium transporting ATPase