title: Endothelial changes and microvascular leakage due to hyperthermia in chick embryos.
abstract: The effects of hyperthermia on the developing 2- and 3-day chick embryo were studied by vital microscopy, in vivo microangiography and electron microscopy of post-capillary venules of the pellucid area of the yolk sac. Hyperthermia of 3 degrees C and 4 degrees C produced significant microvascular changes and perivascular oedema. The microvascular defects were characterized by interruption of the endothelial lining and the presence of blood cells breaking through the vessel walls. In addition, there were numerous inter-endothelial gaps with wide subendothelial spaces. Microangiography showed leakage from the vessel walls. It is concluded that hyperthermia produces vessel wall injury and induces the formation of gaps between endothelial cells resulting in extravasation of plasma and blood cells. These gaps are similar to those produced by biochemical mediators of inflammation. It is suggested that these microvascular changes with pathological leakage may play important roles in abnormal vascular and embryonic development.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004727:Endothelium / Q000473:pathology* / Q000648:ultrastructure; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D008833:Microcirculation / Q000196:embryology* / Q000648:ultrastructure; D014699:Venules / Q000196:embryology / Q000648:ultrastructure; D015017:Yolk Sac / Q000502:physiology* / Q000648:ultrastructure