Distribution of enzymes of fatty acid and ketone body metabolism in periportal and perivenous rat-liver tissue.
The zonal distribution of cytosolic acetyl-CoA carboxylase, mitochondrial 3-hydroxyacyl-CoA dehydrogenase and 3-hydroxybutyrate dehydrogenase was studied in microdissected liver tissue. In fed male and female rats the activity of the lipogenic enzyme acetyl-CoA carboxylase was 1.6-times higher in the perivenous than in the periportal zone of the liver acinus. 3-Hydroxybutyrate dehydrogenase, which is involved in the formation of the ketone body, 3-hydroxybutyrate, exhibited a similar distribution pattern with a 1.5-1.8-times higher activity in the perivenous than in the periportal zone. In contrast, the activity of 3-hydroxyacyl-CoA dehydrogenase, the third enzyme in beta-oxidation, was about equally distributed between the periportal and the perivenous zone of the liver acinus. The results indicate a predominance of lipogenesis in the perivenous, mainly glycolytic zone of the liver acinus. Furthermore, these data support the hypothesis that beta-oxidation supplies energy for basic and anabolic processes like gluconeogenesis in the periportal zone, while it provides acetyl-CoA for ketogenesis besides energy for basic needs in the perivenous zone.
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006503:Hepatic Veins; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D011169:Portal Vein; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology; D014018:Tissue Distribution
D005227:Fatty Acids; D007657:Ketone Bodies; D015094:3-Hydroxyacyl CoA Dehydrogenases; D006884:Hydroxybutyrate Dehydrogenase; D000103:Acetyl-CoA Carboxylase