title: The process in which nucleotide is buried into the active site of heavy meromyosin.
abstract: The process in which nucleotide is buried into the active site of heavy meromyosin was studied with stopped-flow apparatus by monitoring the time-course of the large fluorescence increase of 1,N6-ethenoadenosine triphosphate (epsilon-ATP) when it binds from acrylamide-containing solutions. We have recently reported that free epsilon-ATP fluorescence is effectively quenched by acrylamide while bound epsilon-ATP is resistant to quenching by acrylamide. In the present study it was found that in the first step the phosphate moiety binds at a high rate, while the adenine moiety is still on the rim of the active site; the adenine moiety is then pulled into a crevice, and finally epsilon-ATP hydrolysis occurs.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D017301:Ca(2+) Mg(2+)-ATPase; D007700:Kinetics; D008433:Mathematics; D009132:Muscles / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism*; D011817:Rabbits
chemical_list: D015879:Myosin Subfragments; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase