Further studies of myometrial bradykinin receptor-like binding.
[125I-Tyr1]Kallidin (T1K), a bradykinin (BK) analog with biological potency comparable to BK, was used as a probe for BK receptor-like binding from bovine uterine myometrium. BK binding exhibited a high affinity, Kdissoc = 1.65 X 10(-10) M. The specificity of T1K binding was examined with forty-four BK analogs. Comparison of the binding inhibitory potencies with the relative biological potencies of these analogs on isolated rat uterus resulted in a good correlation, r = 0.87. BK binding activity was solubilized with CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, a zwitterionic detergent. The solubilized binding activity exhibited a BK binding affinity, Kdissoc = 2.25 X 10(-10) M, and a specificity for three 125I-labeled kinins similar to those of the particulate BK receptor-like binding activity.
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D002412:Cations / Q000494:pharmacology; D002417:Cattle; D002793:Cholic Acids / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D005260:Female; D007609:Kallidin / Q000378:metabolism; D009215:Myometrium / Q000378:metabolism*; D018002:Receptors, Bradykinin; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
D002412:Cations; D002793:Cholic Acids; D003902:Detergents; D018002:Receptors, Bradykinin; D011956:Receptors, Cell Surface; D007609:Kallidin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; D001920:Bradykinin