title: Effects of concanavalin A on glutamate operated postsynaptic channels in crayfish muscle.
abstract: Small crayfish muscle fibres were voltage clamped, and synaptic current noise induced by bath application of glutamate was measured. Desensitization of the glutamate receptors was blocked by preincubating the fibres with 0.3-1.0 mumol/l concanavalin A (Con A) for at least 30 min. The power density spectra of the glutamate current noise could be fitted by single component Lorentz curves. The lectin Con A did not influence significantly the conductance gamma of the glutamate channels but increased their mean open time, tau noise. The respective mean values found at T = 8 degrees C and E = -60 mV were gamma = 23.5 +/- 7.0 pS and tau noise = 1.5 +/- 0.2 ms. Both the conductance gamma and the closing rate alpha = tau -1 noise increased with temperature (Q10 approximately 1.9). This temperature dependence was characterized by the activation energies E gamma = 35.2 +/- 7.1 kJ/mol and E alpha = 46.9 +/- 2.1 kJ/mol. The potential dependence of tau noise was almost completely abolished by Con A.
mesh_terms: D000818:Animals; D003400:Astacoidea; D003208:Concanavalin A / Q000494:pharmacology*; D004594:Electrophysiology; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009469:Neuromuscular Junction / Q000502:physiology*; D013570:Synaptic Membranes / Q000502:physiology*
chemical_list: D005971:Glutamates; D007473:Ion Channels; D003208:Concanavalin A; D018698:Glutamic Acid