Coexistence of glutamate decarboxylase and somatostatin immunoreactivity in cultured hippocampal neurons of the rat.
The immunoperoxidase-antiperoxidase method (PAP) was combined with immunofluorescence for simultaneous localization of glutamate decarboxylase (GAD)-and somatostatin-like immunoreactivity in rat hippocampal neurons growing in dissociated cell culture. A subpopulation of GAD-immunoreactive neurons additionally exhibited somatostatin-like immunostaining. GAD-negative somatostatin-positive cells could not be observed. It is discussed whether the cultured somatostatin-containing GAD neurons correspond to a certain subclass of basket cells as they occur in situ.
D000818:Animals; D002478:Cells, Cultured; D005333:Fetus; D005455:Fluorescent Antibody Technique; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D018377:Neurotransmitter Agents / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000378:metabolism*
D018377:Neurotransmitter Agents; D010455:Peptides; D013004:Somatostatin; D005968:Glutamate Decarboxylase