Role of amino acids and endogenous protein in the germination of Mucor racemosus sporangiospores.
The role of amino acids as triggers of Mucor racemosus sporangiospore germination was investigated. No single amino acid was effective as glucose or peptone at triggering germination. Germination induced by glucose or peptone was pH-independent, whereas germination induced by glutamate was pH-dependent. The composition of the free amino acid pools of M-spores (those unable to germinate on glutamate) was qualitatively and quantitatively similar to that of C-spores (those capable of germinating on glutamate) with the exceptions of hydroxyproline and methionine and methionine whose concentrations were several-fold higher in C-spores. Glutamate and leucine were taken up by germinating and nongerminating spores; however, significant protein synthesis occurred only in germinating spores. Spores not triggered by 3-O-methyl-D-glucose (M-spores) contained about one-half the protein of those triggered by 3-O-methyl-D-glucose (C-spores). C-spores initiated to germinate by 3-O-methyl-D-glucose decreased in total organic carbon and protein over a 6 h period; removal of the 3-O-methyl-D-glucose resulted in an immediate halt of protein degradation and spore swelling. These results suggest that protein is a major endogenous reserve in M. racemosus sporangiospores and that its turnover is a necessary event for glucose-triggered germination.
D019325:3-O-Methylglucose; D000596:Amino Acids / Q000378:metabolism* / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000378:metabolism; D008757:Methylglucosides / Q000494:pharmacology; D009089:Mucor / Q000502:physiology*; D013172:Spores, Fungal / Q000502:physiology
D000596:Amino Acids; D005656:Fungal Proteins; D005971:Glutamates; D008757:Methylglucosides; D019325:3-O-Methylglucose; D018698:Glutamic Acid; D007930:Leucine