beta-Adrenergic mechanism of mammalian cells radioprotection by isoproterenol.
In experiments on fibroblasts of Chinese hamster (B II d ii FAF-28, clone 431) it is shown that the specific beta-agonist isoproterenol causes an increase in the intracellular content of cAMP and a decrease in radiosensitivity of the cells. The beta-antagonist propranolol blocks the appearance of the above effects due to isoproterenol. Isoproterenol fails to affect either the intracellular cAMP level or radiosensitivity of mice fibroblasts L, clone B-82, endowed with a functioning adenylate cyclase system but deprived of beta-adrenergic receptors. It is presumed that an increase in radioresistance of mammalian cells due to the effect of isoproterenol is possible only in the presence of beta-receptors in the cells and that it is realized through the beta-adrenergic mechanism.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000378:metabolism; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D011433:Propranolol / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013268:Stimulation, Chemical
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D011837:Radiation-Protective Agents; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol