title: [Glutamate dehydrogenase from rat brain. Properties of the enzyme when acting in the direction of glutamate degradation].
abstract: Several aspects on regulation of rat brain glutamate dehydrogenase when the enzyme catalyses the reaction of glutamate degradation have been studied. The 2-oxo-glutarate is a competitive inhibitor against glutamate and NADH competes with NAD. The enzyme seems to have three sites of binding for glutamate, two of them bind the alpha and gamma carboxylic groups and the other the NH2-group of glutamate. Between the binding of two -COO- groups, the site on the enzyme which binds the gamma -COO- group of glutamate seems to be more important than the alpha one.
mesh_terms: D000494:Allosteric Regulation; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D005971:Glutamates; D007656:Ketoglutaric Acids; D009243:NAD; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D000641:Ammonia; D005969:Glutamate Dehydrogenase
vernacular_title: Glutamato deshidrogenasa procedente de cerebro de rata. Propiedades del enzima cuando functiona en el sentido de la degradación del glutamato.