[Changes in tissue enzymes during "in situ" renal ischemia with or without protection of the kidneys].
Three methods of preservation of the in situ temporarily ischaemic kidney were compared in dogs. The study was carried out in 24 dogs, divided in 4 groups. Each group included 4 dogs in which the ischaemic time was 60 minutes, and 3 dogs in which the ischaemic time was 90 minutes. The experimental animals of the first group served as controls (warm ischaemia); in the second group renal hypothermia was accomplished by an arterial infusion of Collins' solution at 4 degrees C; in the third group preservation of the kidney was attempted only by an arterial infusion of inosine (90-120 mg/kg); finally in the fourth group, the infusion of Collins' solution was combined with infusion of inosine (800-1 000 mg/l Collins' solution). The assessment of the damage of the ischaemic kidney was made by histo-enzymological analysis of renal tissue specimens received from each animal just before initiation of the ischaemia and on the 21st post-operative days. The enzymes examined in this analysis were: Alkaline Phosphatase, ATP-ase, Glutamate Dehydrogenase, Glucose-6-phosphatase, Hydroxybutyrate Dehydrogenase and gamma-GT. These studies have shown that the ischaemic kidney is best preserved by the arterial infusion of Collins' solution with simultaneous infusion of inosine.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004285:Dogs; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007036:Hypothermia, Induced; D007288:Inosine / Q000627:therapeutic use*; D007511:Ischemia / Q000201:enzymology / Q000517:prevention & control*; D007668:Kidney / Q000098:blood supply* / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D007288:Inosine; D006884:Hydroxybutyrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase; D000251:Adenosine Triphosphatases
Etude des variations des enzymes tissulaires au cours de l'ischémie rénale "in situ" associée ou non à des méthodes de protection du rein.