K2-tartarate gradient centrifugation in the isolation of purified influenza virus haemagglutinin after bromelain cleavage.
A method of haemagglutinin (HA) purification by means of K2-tartarate gradient centrifugation is described. Different influenza virus strains possessing the antigenic formulas H1N1, H2N2 and H3N2 yielded pure and immunologically active HA samples.
D001963:Bromelains / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D009975:Orthomyxoviridae / Q000276:immunology*; D013644:Tartrates
D006389:Hemagglutinins, Viral; D013644:Tartrates; D001963:Bromelains; C029768:tartaric acid