Estrogen receptors in human breast cancer. I. Specificity of the histochemical localization of estrogen receptors using an estrogen-albumin FITC complex.
An investigation of the specific localization of estrogen receptors in frozen sections of mammary tumors using an estrogen-BSA-FITC complex is presented. The results are discussed in relation to the existing literature. Although the fluorescence is certainly not due to interaction with type I receptors, the reaction is not as non-specific as is suggested sometimes in the literature. It is likely that type II receptors are involved, which are probably localized at the same site as type I receptors.
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D002417:Cattle; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000494:pharmacology; D005629:Frozen Sections; D006651:Histocytochemistry; D006801:Humans; D009256:Nafoxidine / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology; D013997:Time Factors
D004967:Estrogens; D005452:Fluoresceins; D011960:Receptors, Estrogen; D013861:Thiocyanates; D013629:Tamoxifen; D012710:Serum Albumin, Bovine; D009256:Nafoxidine; D016650:Fluorescein-5-isothiocyanate