Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture.
Ganglion cells were dissociated from postnatal rat retinas, identified by specific fluorescent labels, and maintained in culture on a variety of substrates. Regeneration of processes by retinal ganglion cells was enhanced when the cells were plated on glass coated with a monoclonal antibody against the Thy-1 determinant. Plain glass and glass coated with polylysine, collagen, fibronectin, or other monoclonal antibodies supported the growth of neural processes, but were less effective than antibody to Thy-1.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000954:Antigens, Surface / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D007518:Isoantibodies / Q000502:physiology*; D009416:Nerve Regeneration; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000166:cytology / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007518:Isoantibodies; D018800:Thy-1 Antigens; C035678:anti-Thy antibody; D011107:Polylysine