Calmodulin and the target size of the (Ca2+ + Mg2+)-ATPase of human red-cell ghosts.
An average target size of 251 kDa has been obtained for the (Ca2+ + Mg2+)-ATPase of calmodulin-depleted erythrocyte ghosts by radiation inactivation with 16 MeV electrons. This is close to twice the size of the purified calcium-pump polypeptide. When calmodulin was included during the ATPase assay, a component of about 1 MDa appeared in addition to the activated dimer.
D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases / Q000097:blood* / Q000528:radiation effects; D002147:Calmodulin / Q000097:blood* / Q000528:radiation effects; D004910:Erythrocyte Membrane / Q000201:enzymology* / Q000528:radiation effects; D006801:Humans; D007700:Kinetics
D002147:Calmodulin; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases