In vitro paracrine regulation of islet B-cell function by A and D cells.
In monolayer cultures of islet cells from neonatal rats, incubation of cells for 1 hour with either anti-somatostatin serum or anti-glucagon serum enhanced insulin release. The former appears to be due to neutralization of endogenously secreted somatostatin. The latter may be due to removal of a stimulatory effect of endogenously released glucagon upon somatostatin secretion. Thus, although exogenously added glucagon stimulates insulin secretion, the effect of endogenously released glucagon upon islet B cells is a restraining one which may be mediated through an effect upon D cells and their release of endogenous somatostatin.
D000818:Animals; D002478:Cells, Cultured; D005934:Glucagon / Q000276:immunology / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D007106:Immune Sera / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000276:immunology / Q000502:physiology*
D007106:Immune Sera; D007328:Insulin; D013004:Somatostatin; D005934:Glucagon; D005947:Glucose