Microbial production of L-[15N]glutamic acid and its gas chromatography-mass spectrometry analysis.
L-[15N]Glutamic acid was prepared in high yields via a fermentative process. Brevibacterium lactofermentum, growing on a medium containing 97% enriched 15NH4Cl as a sole isotopic precursor, excreted mostly L-[15N]glutamic acid. The L-[15N]glutamic acid was purified and identified. Gas chromatography-mass spectrometry analysis was performed to demonstrate its usefulness in clinical studies.
D000643:Ammonium Chloride / Q000378:metabolism; D001951:Brevibacterium / Q000378:metabolism*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D005971:Glutamates / Q000096:biosynthesis* / Q000302:isolation & purification; D018698:Glutamic Acid
D005971:Glutamates; D000643:Ammonium Chloride; D018698:Glutamic Acid