Transfer inhibition of RP4 by F factor.
When RP4 and F factors were brought together into one E. coli cell, the F factor reduced 500-1000-fold the frequency of transfer of RP4. However, F had almost no effect on the surface exclusion and pilus formation by RP4. In contrast, RP4 did not affect the transfer of F. Using in vitro recombinant DNA techniques, a gene of F responsible for the above-mentioned transfer inhibition of RP4 was located within the BamHI fragment (40.4-42.8 kb) of the mini-F sequence on F. From the result of product analysis using minicells, the responsible gene in the BamHI fragment was inferred to encode the 33 K protein.
D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D010861:Fimbriae, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids