Intrarenal beta-adrenergic stimulation enhances excretion of urinary lysosomal enzyme in anesthetized dogs.
The effects of intrarenal administration of isoproterenol on excretion of urinary enzymes were examined in dogs. Urinary N-acetyl-beta-D-glucosaminidase (NAG) and glutamyl transpeptidase (gamma-GTP) originated from lysosomes and the brush border membrane of the kidney, respectively. NAG excretion was elevated for 10 min by isoproterenol (0.2 microgram/kg/min) without a drastic change in glomerular filtration rate and renal blood flow. This elevation was inhibited by pretreatment with d,l-propranolol (0.6 mg/kg bolus injection 0.3 mg/kg/30 min) but less so by d-propranolol. Urinary gamma-GTP excretion was little affected by isoproterenol. In light of these findings, the possibility that beta-receptors may be involved in the excretion of NAG has to be considered.
D000818:Animals; D004285:Dogs; D005260:Female; D005919:Glomerular Filtration Rate; D007545:Isoproterenol / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008871:Microvilli / Q000201:enzymology; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D012079:Renal Circulation; D013268:Stimulation, Chemical; D005723:gamma-Glutamyltransferase / Q000652:urine*
D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D005723:gamma-Glutamyltransferase; D007545:Isoproterenol