Cloning and expression in Escherichia coli of a DNA fragment from Bacillus sphaericus coding for biocidal activity against mosquito larvae.
A 3.7 kb DNA fragment from Bacillus sphaericus 1593 was cloned and expressed in E. coli using the plasmid pHV33. The level of larvicidal activity of the hybrid plasmid, pGsp03, against two species of mosquito larvae was comparable to that found with sporulating cell suspensions of B. sphaericus. A limited restriction cleavage map of the cloned insert is given.
D000818:Animals; D001407:Bacillus / Q000235:genetics*; D003001:Cloning, Molecular; D009033:Culicidae; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D007306:Insecticides; D007814:Larva; D010957:Plasmids
D004274:DNA, Recombinant; D007306:Insecticides