Isolation of hybridomas secreting monoclonal antibodies against Physalia physalis (Portuguese man-o'war) nematocyst venom.
Balb/C mice were immunized with crude Portuguese Man-O'War (Physalia physalis) nematocyst venom and their spleen immunocytes were fused with plasmacytoma cells. Nine hybridomas which produced IgG specific for Man-O'War venom were identified using a specific ELISA technique. Ammonium sulfate and DEAE cellulose-purified monoclonal anti-venom antibody had an ELISA titer of 1:4000 and an ability to neutralize the lethal activity (4 LD50/0.6 ml ascites fluid) of an i.v. challenge of crude venom. Indirect immunofluorescence testing demonstrated that the monoclonal antibody isolated in these experiments reacted against a venom component located in the nematocyst wall and thread.
D000818:Animals; D000911:Antibodies, Monoclonal; D003063:Cnidaria / Q000276:immunology*; D003064:Cnidarian Venoms / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D037402:Hydrozoa / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms