title: Dynorphin-related opioid peptides in the neurointermediate pituitary of rats are not alpha-N-acetylated.
abstract: Immunoreactive dynorphin in the neurointermediate pituitary of rats was found to consist of four different molecular weight forms. The three larger molecular weight forms, with apparent molecular weights of 4800, 3200, and 1700, constituted more than 80% of the total dynorphin immunoreactivity, and each liberated leucine-enkephalin but not alpha-N-acetyl-leucine-enkephalin upon enzymatic treatment with trypsin followed by carboxypeptidase B. Only a minor portion of the smallest dynorphin-related molecular weight form, dynorphin-(1-8), released alpha-N-acetyl-leucine-enkephalin upon enzymatic cleavage. This suggests that the vast majority of dynorphin-related peptides in the rat neurointermediate pituitary is not alpha-N-acetylated. The exceptionally high opiate-like activity of the molecular weight 1700 dynorphin suggests that this dynorphin-related opioid peptide may constitute the major part of opioid activity in the neurointermediate pituitary of rats.
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004399:Dynorphins; D004723:Endorphins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004743:Enkephalin, Leucine; D004745:Enkephalins; D007163:Immunosorbent Techniques; D008297:Male; D008970:Molecular Weight; D010446:Peptide Fragments; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004723:Endorphins; D004745:Enkephalins; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; D004399:Dynorphins