Regional assignment of ADA and ITPA to mouse chromosome 2 (C1----ter). A demonstration of the conserved linkage of enzyme and proto-oncogene loci.
Similarity of G-band patterns between the long arm of Chinese hamster chromosome 6 and mouse chromosome 2, combined with the assignments of AK1, ADA, and ITPA to hamster chromosome 6 and AK1 to mouse chromosome 2, suggested mouse chromosome 2 also might contain ADA and ITPA. Here, concordant segregation analysis of enzyme loci and chromosomes in mouse spleen X CHO as well as mouse microcell X CHO somatic cell hybrids established the assignments of ADA and ITPA onto mouse chromosome 2 in the region between the first G-band and the terminus (C1----ter). This assignment presents a demonstration of the conservation and evolution of enzyme and proto-oncogene loci linkage since two cellular homologs of viral oncogenes--c-src and c-abl--also map to mouse chromosome 2. In humans c-src, ADA, and ITPA remain conserved on chromosome 20, whereas AK1 and c-abl are together on chromosome 9. These observations and concepts are discussed with respect to the role of proto-oncogenes in chromosomal evolution and suggest the long arm of chromosome 6 as a fruitful place to look for c-src and c-abl in the Chinese hamster.
D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D005075:Biological Evolution; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics; D008040:Genetic Linkage; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics; D051379:Mice / Q000235:genetics*; D009700:Nucleoside Deaminases / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011755:Pyrophosphatases / Q000235:genetics*; D000097630:Inosine Triphosphatase
D000243:Adenosine Deaminase; D007527:Isoenzymes; D009700:Nucleoside Deaminases; D000090063:Proto-Oncogene Mas; D011755:Pyrophosphatases; D000097630:Inosine Triphosphatase; C000715470:MAS1 protein, human