title: Photobiology of furocoumarins. Various types of crosslinking with DNA and their interference with the development of lambda phage.
abstract: It was shown that the multiplication of phage lambda was strongly suppressed by furocoumarins after irradiation with near ultraviolet light of 365 nm wavelength. Using xanthotoxin or angelicin there was a marked inhibition of the phage DNA injection and replication but adsorption was unaffected. This inhibition was attributed to various types of DNA crosslinking produced in the phage heads. Type I crosslink corresponded to covalent binding between adjacent sites in opposite strands of the double helix. Crosslink type II (hairpin crosslink) required a highly condensed DNA and corresponded to covalent binding between adjacent sites on double-helical segments of a folded DNA molecule. The relationships of the type I crosslinks to the DNA replication and of the type II crosslinks to DNA injection are being discussed. Like type II crosslinks, the nucleic acid--protein crosslinks hinder injection.
mesh_terms: D000327:Adsorption; D010582:Bacteriophage lambda / Q000187:drug effects* / Q000528:radiation effects; D003432:Cross-Linking Reagents; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects; D011564:Furocoumarins / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication / Q000187:drug effects
chemical_list: D003432:Cross-Linking Reagents; D004279:DNA, Viral; D011564:Furocoumarins; D014764:Viral Proteins