Immunosorbent chromatography of sea nettle (Chrysaora quinquecirrha) venom and characterization of toxins.
A lethal toxic fraction from nematocysts of the sea nettle (Chrysaora quinquecirrha) fishing tentacle was partially purified by immunochromatography using an immobilized monoclonal antibody column. Elution from the immunosorbent was accomplished under mild conditions which conserved the biological activity of the toxin. The isolated fraction, which contained two purified protein bands with molecular weights of 100,000 and 190,000 daltons on SDS polyacrylamide gels, was both cardiotoxic and neurotoxic and exhibited an intravenous lethal activity (LD50) of 0.37 microgram/g in mice.
D000818:Animals; D002645:Chickens; D002845:Chromatography; D003064:Cnidarian Venoms / Q000032:analysis* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000187:drug effects; D008970:Molecular Weight; D014118:Toxins, Biological / Q000302:isolation & purification
D003064:Cnidarian Venoms; D014118:Toxins, Biological