title: Vinblastine inhibits the maturation of the precursor of mitochondrial aspartate aminotransferase. Vincristine and six other cytoskeleton inhibitors do not show this effect.
abstract: Cytoskeleton inhibitors were tested in chicken embryo fibroblast cultures for possible effects on the import of the precursor of mitochondrial aspartate aminotransferase into mitochondria. Vinblastine (50 microM) increased the steady-state pool of the precursor 2.5-fold in pulse experiments with [35S]methionine. If the precursor was accumulated during a pulse in the presence of the uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) and then chased under diluting CCCP, vinblastine (50 microM) prolonged the half-life of the precursor from 0.5 min in the control to 3 min. Other cytoskeleton inhibitors, i.e. vincristine (25 to 150 microM), colchicine (50 microM), nocodazole (50 microM), podophyllotoxin (50 microM), taxol (45 microM), cytochalasin D (20 microM) and phalloidin (25 microM) did not show this effect. The observed inhibition by vinblastine does not seem to relate to its action on microtubuli.
mesh_terms: D000470:Alkaloids / Q000633:toxicity; D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics*; D001562:Benzimidazoles / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology; D004792:Enzyme Precursors / Q000235:genetics*; D005347:Fibroblasts / Q000201:enzymology; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D015739:Nocodazole; D010101:Oxygen Consumption / Q000187:drug effects; D017239:Paclitaxel; D010590:Phalloidine / Q000494:pharmacology; D011034:Podophyllotoxin / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D001562:Benzimidazoles; D003572:Cytochalasins; D004792:Enzyme Precursors; D010590:Phalloidine; D015638:Cytochalasin D; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D014747:Vinblastine; D001219:Aspartate Aminotransferases; D011034:Podophyllotoxin; D017239:Paclitaxel; D015739:Nocodazole; D003078:Colchicine