Isolation and convulsivant effect of the "insulin-like" protein obtained from the exocrine pancreas of Bradypus tridactylus L.
1. A pancreatic insulin-like protein fraction with low electrophoretic mobility showing high molecular weight is present. 2. Isoelectric focussing studies showed that the high molecular weight protein has a pI about 7.4 when used at a pH range between 3.5 and 8.0. 3. The partially purified aldehyde-fuchsin positive high molecular weight protein fraction gave a positive effect for the convulsivant test in mice. 4. A high molecular weight insulin-like protein in pancreatic juice was found. 5. Insulin was found by radioimmunoassay in the basal and post stimulation pancreatic juice.
D000818:Animals; D003292:Convulsants; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007328:Insulin / Q000302:isolation & purification* / Q000494:pharmacology; D007525:Isoelectric Focusing; D008297:Male; D010179:Pancreas / Q000032:analysis* / Q000033:anatomy & histology; D010189:Pancreatic Juice / Q000032:analysis; D012896:Sloths / Q000378:metabolism*; D004490:Xenarthra / Q000378:metabolism*
D003292:Convulsants; D007328:Insulin