Effects of inhibitors on monoamine oxidase activity in perch (Perca flavescens) brain in vitro.
1. Perch brain homogenates were incubated in vitro and monoamine oxidase (MAO) activity was determined fluorometrically, using a kynuramine substrate. 2. Clorgyline, harmaline and deprenyl inhibited MAO activity in a concentration-related manner, with single sigmoid inhibition curves, and the type A inhibitors harmaline and clorgyline were more effective than the type B inhibitor deprenyl. 3. Two types of inhibition were recognized in vitro; a fast-onsetting inhibition, similar to that produced by a reversible inhibitor, and a slow-onsetting inhibition, which is time- and concentration-dependent and presumably represents inactivation of the enzyme.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006246:Harmaline / Q000494:pharmacology; D006912:Hydroxyquinolines / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D012642:Selegiline / Q000494:pharmacology; D013997:Time Factors
D006912:Hydroxyquinolines; D008996:Monoamine Oxidase Inhibitors; D012642:Selegiline; D006246:Harmaline; D003010:Clorgyline; C034010:4-hydroxyquinoline