Gamma-glutamyl transpeptidase in a newly established cell line from human renal carcinoma, OUR-10.
A newly established cell line, OUR-10, from human renal carcinoma seems to have the same gamma-glutamyl transpeptidase as the novel one which was found recently in renal carcinoma tissue; the enzymic properties such as electrophoretic mobility, Km value, molecular weight, thermostability, the effects of urea, sodium dodecyl sulfate and sulfhydryl reagents, the effects of amino acids, cations and EDTA, and the binding behavior to a concanavalin A-Sepharose column were the same as those of the novel gamma-glutamyl transpeptidase found in renal carcinoma tissue. The immunological properties of the gamma-glutamyl transpeptidase (GGTP) of OUR-10 examined by inhibition tests and tests of cross-reactivity with anti-normal kidney GGTP antibody were also the same as those of the novel gamma-glutamyl transpeptidase. These results may mean that the novel gamma-glutamyl transpeptidase originates from the cancer cells, and can be used as a marker for identification of this cell line, OUR-10.
D000328:Adult; D000596:Amino Acids / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D002460:Cell Line; D002846:Chromatography, Affinity; D004492:Edetic Acid / Q000494:pharmacology; D004589:Electrophoresis, Disc; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology*; D008970:Molecular Weight; D013696:Temperature; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000596:Amino Acids; D002412:Cations; D004492:Edetic Acid; D005723:gamma-Glutamyltransferase