Incorporation of 14C from glucose into amino acids in brain in vitro in the presence of organo- and inorganic lead and pyridoxal phosphate.
The incorporation of label from U14C glucose into glutamic acid, glutamine and GABA remained unaltered with the presence of lead acetate in the medium whereas tetraethyl lead (TEL) affected the incorporation in a characteristic manner in different regions of brain. Glucose uptake however was not influenced by TEL. Pyridoxal phosphate was found to reverse the effect of TEL on the incorporation especially in cerebellum and brainstem but with little effect in cerebral cortex. These findings suggest that the alterations in the GABA metabolism in TEL toxicity could be restored to some extent by pyridoxine in discrete brain areas.
D000596:Amino Acids / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001933:Brain Stem / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005971:Glutamates / Q000096:biosynthesis; D018698:Glutamic Acid; D005973:Glutamine / Q000096:biosynthesis; D066298:In Vitro Techniques; D007854:Lead / Q000494:pharmacology*; D008297:Male; D009942:Organometallic Compounds / Q000494:pharmacology*; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013756:Tetraethyl Lead / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis
D000596:Amino Acids; D005971:Glutamates; D009942:Organometallic Compounds; D005973:Glutamine; D013756:Tetraethyl Lead; D007854:Lead; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D011732:Pyridoxal Phosphate; D005947:Glucose; C008261:lead acetate