Human Thy-1 antigen: cell surface expression on early T and B lymphocytes.
Human Thy-1 is present on the surface of only a small proportion of haemopoietic cells (thymus--0.2-10%; bone marrow--0.1-0.5%). We have analysed these Thy-1+ cells in more detail using immunofluorescence on a wide range of human haemopoietic cell lines and fresh leukaemic blasts; both situations where rare cells can become clonally expanded. The data demonstrate that Thy-1 is confined to the early stages of T- and B-lymphocyte development, and is absent from all myeloid cells. The Thy-1+ B cells represent late pre-B (c mu+)/early sIgM+ B cells. The Thy-1+ T cells are situated in the outer thymic cortex. Dual immunofluorescence analysis of FACS separated Thy-1+ thymocytes shows them to have the antigenic phenotype and morphology of prothymocytes/early thymocytes (some overlap seen with CALLA, TdT, OKT6 and OKT1).
D000328:Adult; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique; D006801:Humans; D007938:Leukemia / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology
D000954:Antigens, Surface; D018800:Thy-1 Antigens