title: Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production.
abstract: The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid production was completed between 7 and 11 h. Cells grown in the single, chemically defined medium excreted levels of serine and neutral proteases comparable to those excreted in nutrient broth medium.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D010447:Peptide Hydrolases / Q000378:metabolism*; D013171:Spores, Bacterial / Q000201:enzymology / Q000502:physiology
chemical_list: D005971:Glutamates; D007773:Lactates; D019344:Lactic Acid; D018698:Glutamic Acid; D010447:Peptide Hydrolases; D005947:Glucose