Differential assay of choline acetyltransferase activity in human neuroblastomas and cloned adrenergic neuroblastoma of mouse.
A differential assay for the determination of choline acetyltransferase activity in neuroblastoma is described, and the importance of using the differential assay is discussed. In addition, changes in cholinergic-adrenergic activity of cloned adrenergic neuroblastoma cells after serial transplantations are reported. The changes of the cholinergic activity could not be detected precisely without the use of the differential assay.
D000818:Animals; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D002999:Clone Cells; D006801:Humans; D051379:Mice; D008805:Mice, Inbred A; D009374:Neoplasms, Experimental / Q000201:enzymology; D009447:Neuroblastoma / Q000201:enzymology*; D010275:Parasympathetic Nervous System / Q000201:enzymology; D013564:Sympathetic Nervous System / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase