Use of 2-mercaptoethanol during chromatography of crab (Cancer pagurus) metallothionein on DEAE cellulose.
The chromatographic properties of crab metallothionein appear particularly sensitive to oxidation, and this oxidation does not take place when 2-mercaptoethanol is present in the buffers. The chromatographic changes brought about by oxidation can be reversed by subsequent addition of 2-mercaptoethanol.
D000818:Animals; D003386:Brachyura / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D008099:Liver / Q000032:analysis; D008623:Mercaptoethanol; D008668:Metallothionein / Q000302:isolation & purification*; D010179:Pancreas / Q000032:analysis
D008623:Mercaptoethanol; D008668:Metallothionein