title: Demonstration of a herpes simplex virus type 2-induced deoxyuridine triphosphate nucleotidohydrolase in infected KB cells and in biochemically transformed HeLa cells.
abstract: The deoxyuridine triphosphate nucleotidohydrolase (dUTPase) activity which is induced in KB cells infected with herpes simplex virus type 2 (HSV-2) and expressed in biochemically transformed HeLa cells was separated from the host dUTPase activity using either gel filtration or chromatofocusing chromatography. The HSV-2-induced dUTPase differed from the host dUTPase activity in its molecular weight (53000 versus 46000) and isoelectric point (pI 5 X 9 versus 6 X 4). However, it was similar to the host enzyme in that only dUTP served as a substrate for the enzyme.
mesh_terms: D002472:Cell Transformation, Viral; D002850:Chromatography, Gel; D004790:Enzyme Induction; D006367:HeLa Cells / Q000201:enzymology / Q000382:microbiology*; D006561:Herpes Simplex / Q000201:enzymology / Q000382:microbiology*; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011755:Pyrophosphatases / Q000378:metabolism*; D018139:Simplexvirus / Q000201:enzymology*; D014776:Virus Cultivation
chemical_list: D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase