Inhibition of human lymphocyte transformation by two aryloxyalkylamidines.
The incorporation of [3H]thymidine into human lymphocytes stimulated by phytohaemagglutinin (PHA) was inhibited by anilino-N-2-m-chlorophenoxypropylacetamidine (501C) and xylamidine. These amidines antagonize 5-HT, but 5-HT did not alter [3H]thymidine incorporation. 501C inhibited PHA-induced lymphocyte transformation as observed by [3H]thymidine incorporation, [3H]uridine incorporation, [3H]leucine incorporation, DNA content, potassium content, and histological examination. 501C also inhibited increased [3H]thymidine incorporation in human mixed lymphocyte cultures. The IC50 of 501C for inhibition of these processes lay between 4 and 8 microM. When added late in culture (after 6-8 h) 501C was less effective. Possible mechanisms by which 501C inhibits transformation are discussed.
D000578:Amidines / Q000494:pharmacology*; D002860:Chromium Radioisotopes; D004247:DNA / Q000096:biosynthesis / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000578:Amidines; D002860:Chromium Radioisotopes; D010835:Phytohemagglutinins; C029751:BW 501C; D004247:DNA; C005894:xylamidine; D011188:Potassium; D013936:Thymidine