Nature of anthopleurin-B-induced release of norepinephrine from adrenergic nerves.
Anthopleurin-B (AP-B), a new polypeptide from sea anemone (Anthopleura xanthogrammica), markedly increased the amount of norepinephrine (NA) released from the guinea pig isolated vas deferens. The AP-B-induced release of NA was inhibited or abolished by pretreatment with reserpine and by guanethidine or procaine but remained almost unaffected by mecamylamine. D 600, nifedipine, diltiazem, Mn2+, and Mg2+ markedly inhibited the NA releasing action of AP-B. The AP-B-induced release of NA increased in a linear fashion with increasing Na+ concentrations (85-150 mM). Also the NA release by AP-B increased with an increase in the concentration of external Ca2+ from 0 to 0.8 mM but decreased with an increase in the Ca2+ concentration from 0.8 to 2.0 mM. These results suggest that AP-B increases the permeability across the nerve cell membrane to both Na+ and Ca2+ and that this plays an important role in the NA release from the adrenergic nerve.
D000320:Adrenergic Fibers / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003063:Cnidaria; D005711:Gallopamil / Q000494:pharmacology; D006145:Guanethidine / Q000494:pharmacology; D006168:Guinea Pigs; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D012615:Sea Anemones; D013779:Tetrodotoxin / Q000494:pharmacology; D014649:Vas Deferens / Q000294:innervation* / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D005711:Gallopamil; D013779:Tetrodotoxin; D010042:Ouabain; C026132:anthopleurin B; D014700:Verapamil; D011188:Potassium; D009638:Norepinephrine; D006145:Guanethidine