Genetic mapping and transcriptional orientation of the fimD gene.
Expression of type 1 fimbriae in Escherichia coli exhibits phase variation whereby individual cells can alternate between states of organelle expression and nonexpression. Strains carrying fim-lac operon fusions in which lac operon expression is under the control of a fim promoter undergo Lac+ in equilibrium Lac- phase variation. We have determined the genetic map location and direction of transcription of a fim-lac operon fusion which was obtained by insertion of lac into a locus we have named fimD. We found the gene order to be as follows: valS fimD uxuA serB. The direction of transcription of fimD was found to be clockwise on the E. coli chromosome.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D005798:Genes, Bacterial; D009154:Mutation; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004274:DNA, Recombinant