title: A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases.
abstract: Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purify the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000276:immunology*; D000818:Animals; D001360:Avidin; D001710:Biotin / Q000494:pharmacology*; D019735:Carbon-Carbon Ligases; D002262:Carboxy-Lyases / Q000276:immunology*; D002846:Chromatography, Affinity; D006801:Humans; D007223:Infant; D008025:Ligases / Q000276:immunology* / Q000302:isolation & purification; D008722:Methods; D043586:Methylmalonyl-CoA Decarboxylase; D008930:Mitochondria, Liver / Q000201:enzymology*; D011422:Propionates / Q000276:immunology; D011766:Pyruvate Carboxylase / Q000276:immunology*; D011817:Rabbits / Q000276:immunology
chemical_list: D011422:Propionates; D001360:Avidin; D001710:Biotin; D002262:Carboxy-Lyases; D008025:Ligases; D019735:Carbon-Carbon Ligases; D011766:Pyruvate Carboxylase; D000103:Acetyl-CoA Carboxylase; C021573:methylcrotonoyl-CoA carboxylase; D043586:Methylmalonyl-CoA Decarboxylase