[Spatial distribution of thiamine pyrophosphatase and arylsulfatase activities in the area of the Golgi apparatus of the adenohypophyseal secretory cells in the white rat].
Specimens of albino rat pituitary glands were processed consecutively for demonstration of thiamine pyrophosphatase (TPPase) and aryl sulfatase (ArSase) activities. To differentiate between the structures associated with each particular enzyme studied within a single cell, either somatotroph or mammotroph, ultrathin sections were exposed for 2 minutes to 2--50% H2SO4 which removed the reaction products for TPPase rather than those for ArSase. The comparative study of pairs of micrographs of the same area taken before and after the etching with H2SO4 has shown TPPase and ArSase to reside in the Golgi apparatus and GERL system, respectively. Transitional elements have also been discovered, thus supporting the idea that GERL may be a derivative of the Golgi apparatus.
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D005260:Female; D005613:Freeze Etching; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D007774:Lactation; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D011247:Pregnancy; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D013429:Sulfatases / Q000378:metabolism*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*
D013429:Sulfatases; D001192:Arylsulfatases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase
Prostranstvennoe raspredelenie aktivnosteĭ tiaminpirofosfatazy i arilsul'fatazy v oblasti apparate Gol'dzhi sekretornykh kletok adenogipofiza beloĭ krysy.