In situ determination of the molecular weight of hepatic gamma-glutamyl transferase and gamma-glutamyl hydrolase activities.
The molecular weight of gamma-glutamyl transferase from normal rat liver and hepatoma tissue was determined by radiation-inactivation and found to be approx 100000 in each case. The molecular weight previously reported for the subunit containing the gamma-glutamyl binding site (22000) is considerably less than that of the holoenzyme, suggesting that in situ the large subunit is implicated in both transferase and hydrolase activities.
D000818:Animals; D006867:Hydrolases / Q000528:radiation effects; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D011623:gamma-Glutamyl Hydrolase; D005723:gamma-Glutamyltransferase / Q000528:radiation effects
D005723:gamma-Glutamyltransferase; D006867:Hydrolases; D011623:gamma-Glutamyl Hydrolase