Multiple forms of immunoreactive dynorphin in human pituitary and pheochromocytoma.
Multiple forms of immunoreactive dynorphin (I-Dy) in human pituitary and pheochromocytoma were examined utilizing gel filtration and high performance liquid chromatography (HPLC). Gel filtration of I-Dy from these tissues revealed the major component in the position of Dy(1-17) and other minor components with large molecular weight forms. HPLC profile of this major component from gel filtration showed a large peak corresponding to the position of Dy(1-17) and small peaks corresponding to the positions of Dy (1-13), (1-12) and other unknown peptides. These results strongly suggest the presence of Dy(1-17) as the major component, and Dy (1-13), (1-12) or other unknown peptides as the minor components in these human tissues.
D000310:Adrenal Gland Neoplasms / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D009294:Narcotics / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D010673:Pheochromocytoma / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*
D004723:Endorphins; D009294:Narcotics; D010446:Peptide Fragments; C026843:dynorphin (1-13); D004399:Dynorphins; C037422:dynorphin (1-12)