Determination of ethimizol and two of its metabolites in serum by high-pressure liquid chromatography.
A liquid chromatographic assay has been developed for the determination of ethimizol and two of its metabolites in serum. The analysed compounds are preseparated from serum by a micro-column packed with an octadecylsilanized silica gel. Components of the micro-column eluate are analysed on a silica-gel-packed column by means of a high-performance liquid chromatograph fitted with a photometric detector. At 262 nm the detection limit of the injected amount of ethimizol is about 5 ng. The mass spectra of two ethimizol metabolites isolated from rat serum are presented. The suitability of the developed assay for pharmacokinetic studies of ethimizol is demonstrated.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005042:Etimizol / Q000097:blood* / Q000378:metabolism; D006634:Histamine H1 Antagonists / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains
D000893:Anti-Inflammatory Agents; D006634:Histamine H1 Antagonists; D007093:Imidazoles; D005042:Etimizol