Enzyme-linked immunosorbent assay typing of California serogroup viruses isolated in Canada.
A procedure was developed to type California serogroup viruses by an antibody-capture, enzyme-linked immunosorbent assay. Seven California serogroup members from North America were distinguished, including snowshoe hare, La Crosse, California encephalitis, San Angelo, Jamestown Canyon, Keystone, and trivittatus. Extensive cross-reactions were observed between Jamestown Canyon and the closely related South River strain. The enzyme-linked immunosorbent assay method was successfully applied to the typing of 77 California serogroup viruses isolated in Canada, including 61 snowshoe hare, 13 Jamestown Canyon, and 3 trivittatus topotypes.
D000818:Animals; D002043:Bunyaviridae / Q000145:classification*; D002170:Canada; D003429:Cross Reactions; D009033:Culicidae / Q000382:microbiology; D002141:Encephalitis Virus, California / Q000145:classification* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D011817:Rabbits / Q000382:microbiology; D012703:Serotyping / Q000379:methods*