[Oxidation of phenazepam in isolated hepatocytes from white rats].
In isolated rat hepatocytes aromatic hydroxylation and oxidation of the 14C-phenazepame heterocycle were studied. Both these reactions proceeded with the linear rate in intact hepatocytes and aromatic hydroxylation predominated. After induction of cytochrome P-450 by phenobarbital the rate of the heterocycle oxidation was increased 3.6-fold. Rates of aromatic hydroxylation and the phenazepame heterocycle oxidation did not depend on administration of phenobarbital and 3-methyl cholanthrene into the animals. Effects of the factors studied (concentration of phenazepame, induction of cytochrome P-450 by means of phenobarbital and 3-methyl cholanthrene) on alterations in the rate of oxidation of both aromatic nuclei and the phenazepame heterocycle were estimated using dispersion analysis.
D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism*; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004790:Enzyme Induction; D006900:Hydroxylation; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D010084:Oxidation-Reduction; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats
D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D001569:Benzodiazepines; C017928:phenazepam; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital
Okislenie fenazepama v izolirovannykh gepatotsitakh belykh krys.