title: Evidence for the presence of ATP-dependent calcium pump and ATPase activities in bull sperm head membranes.
abstract: Biochemical studies were carried out to demonstrate for the first time direct evidence for the presence of ATP-dependent calcium uptake activity in plasma membrane isolated from the head of bull spermatozoa. The purified plasma membrane vesicles contain also Na+-K+-ATPase, Mg2+-ATPase and Ca2+-ATPase activities. All the activities mentioned were followed in parallel in isolated plasma membranes from the sperm tail. These results together with others, suggest the involvement of the ATP-dependent calcium pump in regulation of intracellular calcium in the process of capacitation and acrosome reaction.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002417:Cattle; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium