A stability-indicating assay for cefotaxime utilizing thin-layer chromatography with fluorescence detection.
A t.l.c. method on silicagel plate with a fluorescence indicator has been carried out to increase the native fluorescence of cefotaxime and four of its degradation products. A simultaneous improved fluorimetric quantitation of the compounds in situ is proposed using a chromatogram spectrophotometer. The detectability (0.20 microgram) shows that the procedure is twenty times more sensitive than high-pressure liquid chromatography with u.v. detection; it allows the determination of traces level (1%) degradation products.
D002439:Cefotaxime / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D004355:Drug Stability; D013050:Spectrometry, Fluorescence / Q000379:methods
D002439:Cefotaxime