Expression of the Thy-1 antigen in long-term cultures of embryonic mouse spinal cord.
Immunofluorescent studies of the Thy-1 antigen in cultures of mouse spinal cord demonstrated positivity on the surfaces of a fraction of neurons and glia. Positivity was evident by one week and increased slowly over the lifespan of the cultures, often several months. Thy-1 positivity was most apparent in regions of clustering of neurons and glia. Neuroglial clusters were identified by morphologic appearance; the identity of astrocytes was confirmed by immunofluorescent staining of glial fibrillary acidic protein.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis*; D001253:Astrocytes / Q000276:immunology; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D051379:Mice; D008810:Mice, Inbred C57BL; D013116:Spinal Cord / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D018800:Thy-1 Antigens