Acetylcholine release elicited by electrical stimulation and its sites of action in smooth muscle of Mytilus.
The effects of strychnine (ST), BTM-1042, hexamethonium (Hexa), tetraethylammonium (TEA), atropine (AT), benactyzine ( Bena ) and dithiothreitol (DTT) on phasic contraction in response to electrical pulses in Mytilus smooth muscle ( ABRM ) have been examined. ST (10(-7) to 5 X 10(-5) M), BTM-1042 (10(-5) to 10(-3) M), Hexa (10(-5) to 10(-3) M) and TEA (10(-5) to 10(-3) M) dose-dependently depressed the contraction. AT (10(-5) to 10(-3) M) had a little influence on the depression of the contraction, while Bena (5 X 10(-6) to 5 X 10(-4) M) dose-dependently depressed the contraction. DTT (10(-4) to 3 X 10(-3) M) depressed the contraction. These findings suggest that the mechanism of acetylcholine-release from excitatory nerve endings is partly similar to its release in the mammalian smooth muscle, and that the cholinergic receptors in ABRM are more like nicotinic receptors than muscarinic ones.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D049872:Bivalvia / Q000502:physiology*; D004229:Dithiothreitol / Q000494:pharmacology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000502:physiology; D011976:Receptors, Muscarinic / Q000187:drug effects; D011978:Receptors, Nicotinic / Q000187:drug effects; D013331:Strychnine / Q000494:pharmacology
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D013331:Strychnine; D000109:Acetylcholine; D004229:Dithiothreitol