Ca2+ transport in erythrocytes from patients with Duchenne muscular dystrophy.
Erythrocyte membranes, isolated after step-wise hemolysis in buffered sucrose (Tris-sucrose membranes), showed higher Ca2+ Mg2+-ATPase activity than erythrocyte membranes obtained after hemolysis in Tris-HCl, and washing in EDTA (Tris-EDTA membranes). In Tris-sucrose membranes, the activity was not stimulated by the addition of monovalent cations, or calmodulin. Differences reported for the activity and properties of Ca2+ Mg2+-ATPase between Tris-EDTA membranes of Duchenne patients and controls, were not found in Tris-sucrose membranes. Also the activity and properties of the Ca2+-pump in resealed erythrocyte membranes were not changed.
D001692:Biological Transport; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D009136:Muscular Dystrophies / Q000097:blood*
D007473:Ion Channels; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium