Analysis of clinical specimens by hybridisation with probe containing repetitive DNA from Plasmodium falciparum. A novel approach to malaria diagnosis.
The capacity of a DNA probe containing cloned repetitive sequences from Plasmodium falciparum to identify malaria-infected blood samples was tested with a spot hybridisation assay. Parasitaemia levels of 0.001% could be detected in 50 microliters blood from patients. The probe correctly diagnosed P falciparum infection in patients from different continents and appeared to be specific for P falciparum, since it did not cross-react with three other Plasmodium species tested.
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII