The de novo biosynthesis of somatostatin and a related peptide in isolated rat dorsal root ganglia.
Rat dorsal root ganglia incorporated [3H]phenylalanine into tetradecapeptide somatostatin as characterized by immunoprecipitation and high performance liquid chromatography. Incorporation increased linearly with time over an 8 h period and was inhibited by anisomycin (10(5) M) implying that it was by a ribosomal mechanism. At early time points [3H]phenylalanine was incorporated into a second peptide which co-eluted with somatostatin-28.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005727:Ganglia, Spinal / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000096:biosynthesis*; D053561:Somatostatin-28
D011498:Protein Precursors; D010649:Phenylalanine; D013004:Somatostatin; D053561:Somatostatin-28