Studies on the structure of avian myeloblastosis virus (AMV) RNA. II. Integrity of the RNA in dependence on RNA isolation and virus propagation.
While sedimentation analysis revealed no basic changes in the sedimentation characteristics of 60-70 S avian myeloblastosis virus (AMV) RNA isolated under different conditions from two sources, electron microscopy exhibited differences in the length distributions of this RNA. Based on the length distribution profiles, phenol extraction showed a higher degradation effect in comparison with direct gradient centrifugation of viral lysates. Proteinase K appeared to be a more suitable nuclease inhibitor than diethylpyrocarbonate during the isolation of AMV RNA. Fundamental differences between length distributions of 60-70 S RNA from plasma AMV and that from AMV propagated in cultured leukaemic myeloblasts were found. Precipitation with ethanol had no effect on the length distribution of AMV RNA. Nor did storage of leukaemic chicken plasma at -70 degrees C and one thawing substantially influence the lengths of AMV RNA. The heterogeneous length distributions of 60-70 S AMV RNA molecules with preferential size-classes were independent of virus propagation and RNA isolation. The results support the idea of preferential splitting of AMV RNA at specific sites.
D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics* / Q000254:growth & development; D002499:Centrifugation, Density Gradient; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D014779:Virus Replication
D012367:RNA, Viral