Commercial immunoperoxidase kits in the study of 13 pancreatic islet-cell tumors.
Use of commercial immunoperoxidase kits for the study of gastrin, glucagon, insulin, and somatostatin production in 13 islet-cell tumors was assessed. Kits save time in preparation and technic, are convenient and easy to use, and are economical for occasional tests. Results are reproducible, with crisp immunostain, minimal background staining, and high sensitivity so that interpretation is easy. The relevant hormone was specifically identified in seven symptomatic tumors. Of six asymptomatic tumors, five showed strongly positive glucagon immunostain and one somatostatin. Nine tumors, in addition, contained somatostatin. Three tumors produced a third hormone. Use of kits can prove expensive for large numbers of tests. Reagents supplied were of unknown dilution and usually insufficient to run negative controls concurrently. Fading of immunostain was noticed after six months of storage.
D007516:Adenoma, Islet Cell / Q000032:analysis*; D005755:Gastrins / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D007328:Insulin / Q000032:analysis; D010190:Pancreatic Neoplasms / Q000032:analysis*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012189:Retrospective Studies; D013004:Somatostatin / Q000032:analysis
D005755:Gastrins; D007328:Insulin; D011933:Reagent Kits, Diagnostic; D013004:Somatostatin; D005934:Glucagon