Histochemical studies on the relationship between the morphology of the Golgi apparatus and G6PD activity in the locus coeruleus and dorsal vagal nucleus of the rat (application of karyometry).
Histochemical studies on the distribution of TPPase and G6PD in the neurons of locus coeruleus (LC) and dorsal vagal nucleus (DVN) of rats have been conducted by using the TPPase method, the G6PD method, karyometry, and statistics under normal conditions. 1. Neurons of the LC and DVN may undergo phasic changes of the GOLGI apparatus (GA) under normal condition. 2. No significant difference was found between neurons of the LC and DVN in regard to the GA morphology, but in general the latter revealed more complicated and developed GA than in the former. 3. Surprisingly, the G6PD activity of LC neurons was very weak. This is in agreement with the previous report by IIJIMA and IMAI (1975), and in disagreement with  FRIEDE (1966). 4. An intimate parallel relationship was found between the GA morphology and G6PD activity in the DVN. The findings support the vigorous synthesizing activity found in the previous study of the rat supraoptic nucleus (IIJIMA 1979). In contrast to the DVN, such a parallel relationship between the Ga shape and G6PD activity was absent in the LC. Both findings strongly suggest that the role of the GA may be functionally different in the LC and DVN.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006651:Histocytochemistry; D007620:Karyometry; D008125:Locus Coeruleus / Q000201:enzymology / Q000648:ultrastructure*; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D014630:Vagus Nerve / Q000201:enzymology / Q000648:ultrastructure*
D005954:Glucosephosphate Dehydrogenase; D013834:Thiamine Pyrophosphatase