The effect of membrane filters on cultured cells.
The use of membrane filters for filtration of serum-containing culture media can change a medium in such a way that cells cultured therein attain a significantly higher lactate-glucose metabolism than the same type of cells cultured in unfiltered medium. Five different filertypes were examined, but the response was only found after filtration with MF-filters of Millipore (mixed esters of cellulose). Furthermore, in two of the three cell lines examined minor growth inhibitions were also observed. The MF-filters were found to contain a compound bound to the filter material so that serum-containing medium, but not serum-free medium, or water, could extract it in amounts sufficient for metabolic - and growth effects. The compound in MF-filters is closely related to Triton-X-100 both with respect to u.v.-spectra and to biological effects.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D000431:Ethanol; D005947:Glucose / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008114:Liver Neoplasms, Experimental; D008851:Micropore Filters; D051381:Rats; D012995:Solubility; D013242:Sterilization / Q000379:methods
D007773:Lactates; D019344:Lactic Acid; D000431:Ethanol; D004247:DNA; D005947:Glucose