Characterization of invertebrate cell lines. IV. Isozyme analyses of dipteran and acarine cell lines.
Cell extracts of five mosquito cell lines and a tick cell line were examined for four cellular isozymes using a cellulose-acetate electrophoretic technique. This method distinguished the cell lines that were derived from the different species. Intraspecies distinctions were not made using the cell lines tested; the significance of this finding is discussed. The usefulness of this technique in identifying a potentially mislabeled cell line was demonstrated.
D000330:Aedes / Q000201:enzymology; D000818:Animals; D000852:Anopheles / Q000201:enzymology; D002460:Cell Line; D002999:Clone Cells; D009033:Culicidae / Q000201:enzymology*; D004175:Diptera / Q000201:enzymology*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D010733:Phosphoglucomutase / Q000032:analysis; D013045:Species Specificity; D013987:Ticks / Q000201:enzymology*
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase