title: Changes in the activity of acetyl-CoA carboxylase during rape-seed formation.
abstract: During the formation of rape-seeds, lipid accumulated in the cotyledons from 16 days after pollination, rising to a plateau after 28 days. The accumulation of lipid was preceded by a marked rise in acetyl-CoA carboxylase activity, which declined rapidly, correlating with the decline in rate of lipid formation. Incubation of rape-seed extracts with avidin-agarose resulted in a decrease in acetyl-CoA carboxylase activity in the extract. Polyacrylamide-gel electrophoresis of polypeptides bound to avidin-agarose showed the presence of a polypeptide of Mr 225 000. The intensity of this band increased during the period of increase of acetyl-CoA carboxylase activity in the seeds.
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D001360:Avidin / Q000378:metabolism; D001937:Brassica / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D008025:Ligases / Q000378:metabolism*; D050356:Lipid Metabolism; D008854:Microscopy, Electron; D010940:Plant Proteins / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000648:ultrastructure; D012685:Sepharose / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D001360:Avidin; D012685:Sepharose; D008025:Ligases; D000103:Acetyl-CoA Carboxylase