title: Electron capture GLC analysis of the thienodiazepine clotiazepam. Preliminary pharmacokinetic studies.
abstract: Plasma concentrations of 5-(2-chlorophenyl)-7-ethyl-1,3-dihydro-1-methyl-2H-thieno[2,3-e][1,4]diazepin-2 -one (clotiazepam, CTZ, Trecalmo), a thienodiazepine anti-anxiety agent, and two of its metabolites (hydroxy- and desmethyl-clotiazepam) are reliably quantified by electron capture GLC. After addition of diazepam as internal standard, 1-ml plasma samples are extracted with benzene-isoamyl alcohol (98.5:1.5). The organic extract is evaporated to dryness, reconstituted, and directly chromatographed using a 3% SP-2250 liquid phase at 260 degrees C. Sensitivity limits are 1-3 ng of each of the 3 compounds per ml of plasma. The method is applicable to clinical pharmacokinetic studies in humans. After p.o. ingestion of a single 5-mg dose of clotiazepam by three healthy volunteers the drug was rapidly absorbed. Values of elimination half-life were 6.5, 7.0, and 17.8 h, respectively. The two metabolites appeared and disappeared approximately in parallel with the parent compound. Clotiazepam was more than 99% bound to plasma protein.
mesh_terms: D000328:Adult; D001381:Azepines / Q000008:administration & dosage / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D007700:Kinetics; D008297:Male; D011485:Protein Binding; D012995:Solubility; D013607:Tablets
chemical_list: D001381:Azepines; D001798:Blood Proteins; D013607:Tablets