title: Thyroid lysosomal enzyme activity and ultrastructure after subtotal thyroidectomy.
abstract: Subtotal thyroidectomies were performed in rats to increase the level of endogenous TSH, creating a condition of chronic TSH stimulation. The activities of various classes of lysosomal enzymes (cathepsin D, beta-glucuronidase, and aryl sulfatase A) were studied in thyroid tissue remaining in situ at various time intervals after subtotal thyroidectomy (sub-tx). These alterations were correlated with morphometric and ultrastructural changes in tissue lysosomes and with serum T4 and TSH. Specific activities of all three lysosomal enzymes were elevated in the residual tissue as compared with those in control tissue during 7 weeks after sub-tx in the first experiment. In the second experiment, the activities of all three enzymes were elevated both 3 and 6 weeks after sub-tx, and the activities of cathepsin D and aryl sulfatase A in the postnuclear homogenate (S2) were significantly elevated. Plasma TSH was elevated and T4 was decreased both 3 and 6 weeks after sub-tx. The results of the third experiment determined that there were significant alterations in nuclear cytoplasmic ratios as well as in the number, area, and volume density of lysosomes in both groups compared with respective control values. In addition, both lysosomal area and volume density in animals 6 weeks after sub-tx were significantly larger than those in animals 3 weeks after sub-tx. We conclude that chronic stimulation of residual thyroid tissue 6 weeks after sub-tx causes alterations in lysosomal ultrastructure as well as in lysosomal enzyme activity.
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism*; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013429:Sulfatases / Q000378:metabolism*; D013961:Thyroid Gland / Q000201:enzymology* / Q000648:ultrastructure; D013965:Thyroidectomy; D013972:Thyrotropin / Q000502:physiology; D013974:Thyroxine / Q000097:blood; D013997:Time Factors
chemical_list: D013972:Thyrotropin; D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D005966:Glucuronidase; D002402:Cathepsin D; D013974:Thyroxine