Enzymatic transfer of galactosyl phosphate from UDP-galactose to UDP-N-acetylglucosamine.
The microsomal fraction of hen oviduct homogenate has been shown to contain an enzyme capable of catalyzing a transfer of galactosyl phosphate from UDP-galactose to UDP-N-acetylglucosamine. The product was isolated and identified as UDP-N-acetylglucosamine-6-phosphogalactose, the same compound as that found as a normal constituent in hen oviduct. The enzyme is analogous in reaction type to UDP-N-acetylglucosamine: glycoprotein N-acetylglucosamine-1-phosphotransferase (the enzyme responsible for introducing the recognition marker of newly synthesized lysosomal enzymes), which suggests that the galactosyl phosphotransferase is involved in galactose 1-phosphate transfer to N-acetylglucosamine residues of newly synthesized glycoproteins.
D000469:Alkaline Phosphatase / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D005260:Female; D005695:Galactosephosphates / Q000378:metabolism*; D006600:Hexosephosphates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008861:Microsomes / Q000201:enzymology; D010057:Oviducts / Q000201:enzymology; D010770:Phosphotransferases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000494:pharmacology; D014018:Tissue Distribution; D017855:Transferases (Other Substituted Phosphate Groups); D014531:Uridine Diphosphate Galactose / Q000378:metabolism*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D005695:Galactosephosphates; D006600:Hexosephosphates; D014539:Uridine Diphosphate Sugars; C029973:galactose-1-phosphate; D014531:Uridine Diphosphate Galactose; D014537:Uridine Diphosphate N-Acetylglucosamine; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C037363:UDPgalactose - UDP-N-acetylglucosamine galactosephosphotransferase; D000469:Alkaline Phosphatase; D011755:Pyrophosphatases; C021899:nucleotide pyrophosphatase