Potentiation by sulphydryl agents of the responses of guinea-pig isolated ileum to various agonists.
Sulphydryl agents (dithiothreitol, mercaptoethanol, monothioglycerol, cysteine, glutathione) increase non-specifically the potency of various agonists (acetylcholine, 5-hydroxytryptamine, nicotine, bradykinin, prostaglandin E2) on the guinea-pig ileum. These effects are of non-cholinergic origin and are not related to prostaglandin synthesis. Sulphydryl agents act directly on the smooth muscle cells of the guinea-pig ileum most probably by reducing disulphide bonds located on the membrane surface and by this mechanism modulate the muscular contractile activity.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003545:Cysteine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004357:Drug Synergism; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D005990:Glycerol / Q000031:analogs & derivatives / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol; D009538:Nicotine; C009465:thioglycerol; D005978:Glutathione; D003545:Cysteine; D000109:Acetylcholine; D005990:Glycerol; D004229:Dithiothreitol