The role of chloroplast and cytoplasm in the NADP-glutamate dehydrogenase and glutamine synthetase synthesis in Ankistrodesmus cells.
The effects of transcription and translation inhibitors on NADP-glutamate dehydrogenase and glutamine synthetase synthesis in nitrogen-starving Ankistrodesmus braunii cells have been studied. Considering the results obtained one can suggest that both enzymes are coded in a chloroplast genome and that during nitrogen starvation specific mRNA's are partly transferred from chloroplast into cytoplasm and can be translated there on 80S ribosomes.
D002701:Chloramphenicol / Q000494:pharmacology; D002736:Chloroplasts / Q000201:enzymology*; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis*; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis*; D009249:NADP / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*
D009249:NADP; D002701:Chloramphenicol; D003513:Cycloheximide; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase