Cobalt-ions dissociate between calcium uptake through voltage-dependent sodium and calcium channels in synaptosomes.
Calcium ions are taken up through at least two separate, voltage-dependent channels in rat cortical synaptosomes. One channel is opened by 60 mM K+, blocked completely with 500 microM Co2+ and unaffected by 1 microM tetrodotoxin; the other is opened by 20 microM veratridine in a sodium-independent manner, blocked by 1 microM tetrodotoxin, and only partially inhibited by Co2+. Calcium-uptake through either channel is functional with respect to releasing [3H]ACh.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003035:Cobalt / Q000378:metabolism*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D007473:Ion Channels; D018377:Neurotransmitter Agents; D003035:Cobalt; D012964:Sodium; D002118:Calcium