Characterization of benzo[a]pyrene metabolites formed by 3-methylcholanthrene-induced goldfish, black bullhead and brown bullhead.
Metabolites of BaP formed by 3-MC-induced goldfish (Carassius auratus), black bullhead (Ictalurus melas), brown bullhead (Ictalurus nebulus) and rat have been separated by HPLC. The main metabolites in both bullhead species and the rat were 3-hydroxy-BaP and BaP 9,10- and 7,8-dihydrodiols, whereas BaP 1,6- and 3,6-diones and 9- and 3-hydroxy-BaP predominated in the goldfish. Induced rat formed over 10 times as many total metabolites as the fish. No induction was observed in the 3-MC-treated brown bullheads kept at 7 degrees C. TCPO and alpha-naphthoflavone decreased metabolite production by 50 and 30%, respectively.
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001571:Benzoflavones / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005399:Fishes / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D011083:Polycyclic Compounds / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014239:Trichloroepoxypropane / Q000378:metabolism
D001571:Benzoflavones; D011083:Polycyclic Compounds; D014239:Trichloroepoxypropane; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; C011512:alpha-naphthoflavone; D006899:Mixed Function Oxygenases