Cells infected with human cytomegalovirus release a factor(s) that stimulates cell DNA synthesis.
Culture supernatants of permissive and non-permissive cells infected with human cytomegalovirus (HCMV) contain a growth factor that enhances the DNA synthesis and mitotic activity of target cells. This cytomegalovirus growth factor CMV-GF) is a heat-stable, acid-labile polypeptide that is sensitive to trypsin and dithiothreitol. CMV-GF is an early product of the infected cells and defective virions are primarily responsible for its induction. Microtubule depolymerization is necessary for the induction of DNA synthesis by the CMV-GF, since taxol, an inhibitor of microtubule depolymerization, blocks its effect.
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002645:Chickens; D003587:Cytomegalovirus / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D004229:Dithiothreitol / Q000494:pharmacology; D006133:Growth Substances / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D017239:Paclitaxel; D014357:Trypsin / Q000378:metabolism; D014771:Virion / Q000032:analysis
D000470:Alkaloids; D006133:Growth Substances; D014357:Trypsin; D017239:Paclitaxel; D004229:Dithiothreitol