Poly A (+) mRNA metabolism in wing imaginal discs during normal development and diapause in Pieris brassicae.
Poly A (+)mRNA synthesis was analyzed during the nymphal stage and the diapause in the wing discs of the Lepidopteran Pieris brassicae. The main events of differentiation, i.e. scale formation, adult cuticle elaboration and pigment deposition, occurred during the period studied. We only detected one phase of synthesis for poly A (+)mRNA molecules, 48-72 hours after the nymphal moult. This synthesis was found to be related to that of late proteins at 120-140 hours. Examination of mRNA metabolism in discs treated with cordycepin (3' dA) showed a decline in mRNA stability. This decline corresponded to a turn-over phase followed by a period of stabilization which preceded mRNA translation. In diapausing animals, poly A (+)mRNA metabolism was unexpectedly high, and many messengers were synthesized and rapidly destroyed. These messengers were found to be slightly heavier than the mRNAs produced during normal development, suggesting a blocking at some step in their maturation. We developed a mathematical model for mRNA metabolism which enabled us to calculate the effects of synthesis and degradation on the quantity of mRNAs, from the poly A (+)mRNA concentration and the turnover time in the mRNA pool. In addition determination phases associated with embryological events and terminal differentiation are clearly distinguished. This feature offers opportunities to investigate the commitment events which take place during the end of the larval stage and the beginning of the nymphal stage.
D000818:Animals; D002080:Butterflies / Q000254:growth & development / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D007915:Lepidoptera / Q000378:metabolism*; D008433:Mathematics; D008954:Models, Biological; D009758:Nymph / Q000378:metabolism; D011061:Poly A / Q000096:biosynthesis*; D011679:Pupa / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D014921:Wings, Animal / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A