[Submicroscopic study of adenohypophyseal cells with the hypophysis incubated in a hypertonic solution. I. The distribution of acid phosphatase, thiamine pyrophosphatase and arylsulfatase activities].
In the rat adenohypophyseal mammotrophs and somatotrophs, activities of acid phosphatase, thiamine pyrophosphatase and aryl sulphatase were defined after the incubation of the pituitary gland in the medium containing 1.65 M sucrose. Following 60--120 minutes of the incubation the above enzymes were found, in addition to their usual sites, in all cisternae of the Golgi apparatus and the rough endoplasmic reticulum as well as within the perinuclear cisternae. The mechanism of the above alterations is discussed, and a suggestion is put forward that the emergence of enzymatic activities in the above structures may reflect their biogenetic pathways in the cell.
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D005260:Female; D006982:Hypertonic Solutions; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000494:pharmacology; D013429:Sulfatases / Q000378:metabolism*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*; D013997:Time Factors
D006982:Hypertonic Solutions; D013395:Sucrose; D000135:Acid Phosphatase; D013429:Sulfatases; D001192:Arylsulfatases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase
Submikroskopicheskoe issledovanie adenogipofizarnykh kletok pri inkubatsii gipobiza v gipertonicheskom rastvore. I. Raspredelenie aktivnosteĭ kisloĭ fosfataze, tiaminpirofosfatazy i arilsul'fatazy.