Isolation of crustacean erythrophore concentrating hormone from nerve tissue of Homarus americanus.
Crustacean erythrophore concentrating hormone (CECH) was isolated from lobster (Homarus americanus) eyestalks by reverse phase high-performance liquid chromatography (rp-hplc). Putative lobster CECH fractions co-chromatographed with commercially available pure CECH on two independent rp-hplc systems, and were positive for activity by bioassay. CECH was detected in eyestalk, but not brain homogenates.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007447:Invertebrate Hormones / Q000302:isolation & purification*; D008121:Nephropidae / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009842:Oligopeptides / Q000302:isolation & purification*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid