title: Functional importance of the synaptic plasma membrane calcium pump and sodium-calcium exchanger.
abstract: Two major Ca2+ transport mechanisms co-function in a preparation of synaptosomal plasma membrane vesicles: an (ATP + Mg2+)-dependent Ca2+ pump, and a reversible Na+-Ca2+ exchanger (Gill, D. L., Grollman, E.F., and Kohn, L. D. (1981) J. Biol. Chem. 256, 184-192). An accurate comparative analysis of the kinetics of the two Ca2+ transporters under free Ca2+ conditions precisely buffered with EGTA, reveals that both mechanisms have high affinity for Ca2+. The ATP-dependent Ca2+ pump displays simple saturation kinetics with a Km for Ca2+ of 0.11 microM and a Vmax of 2.2 nmol/min/mg of protein. In contrast, the Na+-Ca2+ exchanger has a complex dependence on free Ca2+, the activity continuing to saturate over a wide range of free Ca2+ concentrations from 0.03 microM to 3 mM. The curvilinear Eadie-Hofstee analysis reveals a distinct high affinity component for the exchanger with a Km for Ca2+ of approximately 0.5 microM, and a lower affinity component not accurately resolvable into a discrete Km value. 2 mM amiloride blocks Na+-Ca2+ exchange-mediated Ca2+ uptake by 90% over a wide range of free Ca2+ (0.3-3000 microM), suggesting a similar noncompetitive inhibition of both low and high affinity Ca2+ sites. Ca2+ accumulated in vesicles via either the Ca2+ pump or Na+-Ca2+ exchanger is rapidly (in less than 1 min) released by 0.1% saponin (w/v), although a minor component (8-10%) of Ca2+ pump activity is resistant to saponin addition. The IC50 for the effect of saponin is the same (0.01%, w/v) for both Ca2+ transport mechanisms. The ATP-dependent Ca2+ pump is shown to be highly sensitive to vanadate inhibition (Ki = 0.5 microM). The high saponin sensitivity of both Ca2+ transporters and the potent effect of vanadate on Ca2+ pumping, together with previous Na+ channel and Na+ pump flux studies in the same membrane vesicles (Gill, D. L. (1982) J. Biol. Chem. 257, 10986-10990), all strongly suggest that both of the high affinity Ca2+ transporters function in the plasma membrane where they are of major functional importance to the regulation of intrasynaptic free Ca2+ levels.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002540:Cerebral Cortex / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D051381:Rats; D012503:Saponins / Q000494:pharmacology; D013570:Synaptic Membranes / Q000201:enzymology*; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
chemical_list: D012503:Saponins; D014639:Vanadium; D014638:Vanadates; D000584:Amiloride; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C034688:sodium calcium ATPase; D000252:Calcium-Transporting ATPases