Rapid formation of poly-gamma-glutamyl derivatives of methotrexate and their association with dihydrofolate reductase as assessed by high pressure liquid chromatography in the Ehrlich ascites tumor cell in vitro.
D000818:Animals; D001692:Biological Transport; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005492:Folic Acid / Q000031:analogs & derivatives*; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D005973:Glutamine / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008727:Methotrexate / Q000378:metabolism; D051379:Mice; D011624:Pteroylpolyglutamic Acids / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism*
D005971:Glutamates; D011624:Pteroylpolyglutamic Acids; D005973:Glutamine; D018698:Glutamic Acid; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate