Peroxisomal fatty acyl-coenzyme A oxidation in chicken liver.
The activities of antimycin A-insensitive palmitoyl-CoA oxidation and of palmitoyl-CoA oxidase in peroxisomes from chicken liver were similar to those of rat liver. Catalase and D-amino acid oxidase activities in peroxisomes from chicken liver were lower than those of rat liver, and urate oxidase was not detected. Carnitine acetyl-transferase and palmitoyltransferase levels in chicken liver were 18- and 2-fold higher, respectively, than those of rat liver. Peroxisomal palmitoyl-CoA oxidation of chicken liver was inhibited by cyanide, in contrast to that of rat liver, although it was insensitive to antimycin A. Subcellular distribution of this enzyme was similar to that of rat liver; i.e., it was located only in the peroxisomes. The fatty acyl-CoA oxidase had a higher affinity toward medium- to long-chain fatty acyl-CoAs (C8 to C16) than shorter-chain analogs. The fatty acyl-CoA dehydrogenase had a broad affinity toward fatty acyl-CoAs (C4 to C18). Carnitine acetyltransferase was distributed equally in both peroxisomes and mitochondria. Carnitine palmitoyltransferase was distributed in the proportion of 20 and 80% in peroxisomes and mitochondria, respectively.
D000123:Acetyltransferases / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D002645:Chickens; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008830:Microbodies / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; D000968:Antimycin A; D010088:Oxidoreductases; D002374:Catalase; C028427:palmitoyl CoA oxidase; D003605:D-Amino-Acid Oxidase; D000123:Acetyltransferases; D002332:Carnitine O-Acetyltransferase; D011190:Potassium Cyanide