Hepatic microsomes from freshwater fish--I. In vitro cytochrome P-450 chemical interactions.
1. Of 87 chemicals tested for their ability to interact with oxidized hepatic cytochrome P-450 from mature male brook trout (Salvelinus fontinalis), 21 formed detectable type I or type II binding spectra. 2. When 8 of these 21 chemicals were tested with cytochrome P-450 of nine other species of freshwater fish, wide species variation in hepatic microsomal cytochrome P-450 was evident, since the spectral size of chemical interactions as related to the carbon monoxide spectrum and the ratio of type II to type I binding were not alike. 3. These spectral data suggest that hepatic microsomal cytochrome P-450 of freshwater fish exists in different forms.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology*; D011485:Protein Binding; D014337:Trout / Q000378:metabolism
D003577:Cytochrome P-450 Enzyme System