Binding of [3H]mianserin to bovine serum albumin, human serum albumin and alpha 1-acid glycoprotein.
Scatchard plots which were curvilinear with negative slopes were obtained when the binding of [3H]mianserin to bovine serum albumin (BSA), human serum albumin (HSA), defatted human serum albumin (D-HSA) and alpha 1-acid glycoprotein (alpha 1-AGP) was studied with equilibrium dialysis with constant protein concentrations and various ligand concentrations. Binding parameters were estimated graphically and with a non-linear least-squares computer program, assuming two classes of independent binding sites. alpha 1-AGP had the highest binding affinity (K) and binding capacity (nK). The binding parameters, n and K were not independent of protein concentration when the BSA concentration was varied. Linear atypical Scatchard plots with positive slopes were obtained when the protein concentration was varied for BSA, HSA and D-HSA, at a fixed ligand concentration.
D000818:Animals; D002417:Cattle; D003984:Dibenzazepines / Q000378:metabolism*; D006801:Humans; D008803:Mianserin / Q000378:metabolism*; D009961:Orosomucoid / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D014316:Tritium
D003984:Dibenzazepines; D009961:Orosomucoid; D012709:Serum Albumin; D014316:Tritium; D008803:Mianserin; D012710:Serum Albumin, Bovine