Behaviour of tyrosine amino transferase and convertase during the first hours after hepatectomy in rats.
The activity of rat liver tyrosine amino transferase (TAT) increases after hepatectomy with a first prominent peak at 8 h and a second peak at 18 h. This change in activity is probably due to de novo enzyme synthesis since it is prevented by actinomycin-D (AMD). In the same period an increase of the lysosomal converting enzyme (convertase) which catalyses the in vitro transition of TAT from form I to form III, has been observed; this is not accompanied by changes of other lysosomal enzymes, such as acid phosphatase and cathepsin L. The activity of convertase is equal to that of the controls (sham operated animals) 2 h after hepatectomy, increases three times at 5 h, maintains the same value at 8 h and then decreases slowly to control level after 24 h. The correlation between the activity changes of the two enzymes strongly suggests a physiological role of convertase in TAT turnover.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D003546:Cysteine Endopeptidases; D003609:Dactinomycin / Q000494:pharmacology; D010450:Endopeptidases; D006498:Hepatectomy; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008115:Liver Regeneration; D008247:Lysosomes / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014444:Tyrosine Transaminase / Q000378:metabolism*
D003609:Dactinomycin; D014444:Tyrosine Transaminase; D000135:Acid Phosphatase; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534280:Ctsl protein, rat; C031041:cathepsin T