Supersensitivity of the GABA receptor in the frog spinal cord, as induced by kainic acid.
In the isolated frog spinal cord perfused with kainic acid (KA, 5 X 10(-4) M) containing Ringer's solution, within 2 hr there were increases in the amplitude of the dorsal root depolarization, as induced by the GABA-agonists. KA perfusion produced increases in the specific binding of [3H]muscimol to crude synaptic membranes and incubation with KA for 3 hr did not increase [3H]muscimol binding. [3H]GABA was released from KA-treated spinal cord slices in the presence of high K+. KA-induced supersensitivity of the dorsal root to GABA may relate to direct actions on primary afferent terminals and not to denervation of GABAergic neurons.
D000818:Animals; D004594:Electrophysiology; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology*; D009118:Muscimol / Q000378:metabolism; D010477:Perfusion; D011759:Pyrrolidines / Q000494:pharmacology*; D011892:Rana catesbeiana; D011956:Receptors, Cell Surface / Q000502:physiology*; D011963:Receptors, GABA-A; D013116:Spinal Cord / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D011759:Pyrrolidines; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D007608:Kainic Acid