Absence of specific binding of several putative neuro-transmitters to human fibroblasts.
Fibroblasts were examined for specific binding sites of ten putative neurotransmitters to determine whether this tissue could be used in receptor studies of neurologic and psychiatric disorders. Stereospecific saturable binding was not found for any of the ligands: arginine vasopressin, neurotensin, somatostatin, angiotensin II, thyrotropin-releasing hormone (TRH), alpha-bungarotoxin, LSD, dihydromorphine, muscimol and spiperone.
D000804:Angiotensin II / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011945:Receptors, Angiotensin / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism; D011963:Receptors, GABA-A; D018028:Receptors, Neurotensin; D011978:Receptors, Nicotinic; D011957:Receptors, Opioid / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D017481:Receptors, Somatostatin; D011988:Receptors, Thyroid Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D064569:alpha7 Nicotinic Acetylcholine Receptor
C579167:Chrna7 protein, human; D018377:Neurotransmitter Agents; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D011954:Receptors, Dopamine; D011963:Receptors, GABA-A; D018028:Receptors, Neurotensin; D011978:Receptors, Nicotinic; D011957:Receptors, Opioid; D011985:Receptors, Serotonin; D017481:Receptors, Somatostatin; D011988:Receptors, Thyroid Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D064569:alpha7 Nicotinic Acetylcholine Receptor; D000804:Angiotensin II