Role of phosphatases in the biosynthesis of chlortetracycline in Streptomyces aureofaciens.
ATP diphosphohydrolase activity and inorganic pyrophosphatase reached two maxima during cultivation of the low- and high-producing variant of Streptomyces aureofaciens under conditions of phosphate limitation, i.e. after 30 and 70 h of cultivation. Increased levels of inorganic phosphate in a medium inhibitory to biosynthesis of chlortetracycline markedly decreased the levels of both enzymes. The ATP diphosphohydrolase activity was detected both in the supernatant and membrane fractions of the cell-free preparation of the mycelium.
D001081:Apyrase / Q000378:metabolism*; D002751:Chlortetracycline / Q000096:biosynthesis*; D003470:Culture Media; D008566:Membranes / Q000201:enzymology; D010710:Phosphates / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*
D003470:Culture Media; D010710:Phosphates; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; D001081:Apyrase; D002751:Chlortetracycline