Increase of ovarian progesterone secretion by beta 2-adrenergic stimulation in oestrous rats.
Oestrous rats were anaesthetized with pentobarbital and one of the femoral arteries, femoral veins and utero-ovarian veins were cannulated. Five min blood fractions were collected from the ovary for 50 min. Following two control fractions fenoterol, noradrenaline, isoproterenol (0.5 microgram/min) or 0.9% NaCl (0.02 ml/min) were infused iv for 40 min. In a group of oestrous animals fenoterol was given locally to the ovarian bursa. Blood pressure and the ovarian venous outflow were continuously recorded and blood levels of progesterone (P) and oestradiol-17 beta (E2) were determined by RIA. Fenoterol administered iv increased P secretion without altering ovarian blood flow, whereas noradrenaline and isoproterenol had no effect on P secretion. Fenoterol administered locally stimulated both P and E2 secretion, and this was prevented by iv infusion of propranolol. It is suggested that ovarian beta 2-adrenergic receptors have a regulatory role in ovarian hormone secretion.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004958:Estradiol / Q000097:blood / Q000378:metabolism; D004971:Estrus; D005260:Female; D005280:Fenoterol / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010053:Ovary / Q000098:blood supply / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D014655:Vascular Resistance / Q000187:drug effects
D000318:Adrenergic beta-Agonists; D005280:Fenoterol; D011374:Progesterone; D004958:Estradiol; D007545:Isoproterenol; D009638:Norepinephrine