[Investigation of the lipid dependence of pyrophosphatase activity from rat liver microsomes and hepatoma by phospholipase C].
The lipid dependence of pyrophosphatase activity was studied by treatment of liver and hepatoma microsomes with phospholipase C from Cl. perfringens and B. cereus and a subsequent incorporation of various classes of phospholipids into the delipidated microsomes. Phospholipase C hydrolysis sharply lowers the pyrophosphatase activity of liver and hepatoma microsomes. The enzyme activity is restored after introduction of phospholipids into delipidated liver microsomes, the maximal effect being achieved on incorporation of phosphatidylcholine. All the phospholipids tested exerted the same reactivation effects on the delipidated microsomes of hepatoma. However, a more complete delipidation of hepatoma microsomes by phospholipase C hydrolysis and a subsequent organic solvent extraction revealed a specific dependence of the enzyme activity on phosphatidylserine.
D000818:Animals; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000494:pharmacology / Q000502:physiology; D010718:Phosphatidylserines / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology*; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D010738:Type C Phospholipases / Q000494:pharmacology*
D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids; D010740:Phospholipases; D010738:Type C Phospholipases; D011755:Pyrophosphatases
Issledovanie lipidnoĭ zavisimosti pirofosfataznoĭ aktivnosti mikrosom pecheni i gepatomy krys s pomoshch'iu fosfolipazy C.