Hydrophilic form of gamma-glutamyltransferase: proteolytic formation in liver homogenates and its estimation in serum.
By incubating liver homogenates at pH 7.0 a proteolytic modification of gamma-glutamyltransferase was demonstrated. The amphiphilic, membrane-bound enzyme was converted to a hydrophilic form whose size and electrophoretic mobility were identical to those of the hydrophilic form present in human serum. The relative amounts of the hydrophilic and the amphiphilic gamma-glutamyltransferase in various pathological sera were analyzed. In the majority of these sera the hydrophilic form constituted less than 15-20% of the total activity. High amounts (30-55%) were demonstrated in serum samples from two patients with necrosis of the liver. However, the determination of this form of gamma-glutamyltransferase appears to have little clinical significance.
D002850:Chromatography, Gel; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000097:blood*
D005723:gamma-Glutamyltransferase; D010447:Peptide Hydrolases