DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression.
Colchicine-resistant Chinese hamster ovary (CHO) cell mutants whose resistance results from reduced drug permeability have been isolated previously in our laboratories. This reduced permeability affects a wide range of unrelated drugs, resulting in the mutants displaying a multiple drug resistance phenotype. A 170,000-dalton cell surface glycoprotein (P-glycoprotein) was identified, and its expression appears to correlate with the degree of resistance. In this study we were able to confer the multiple drug resistance phenotype on sensitive mouse L cells by DNA-mediated gene transfer of DNA obtained from the colchicine-resistant mutants. P-glycoprotein was detected in plasma membranes of these DNA transformants by staining with an antiserum raised against membranes of mutant CHO cells. These results are consistent with a causal relationship between P-glycoprotein expression and the multiple drug resistance phenotype.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004351:Drug Resistance; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics*; D007739:L Cells / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D010641:Phenotype; D014170:Transformation, Genetic
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D006023:Glycoproteins; D008565:Membrane Proteins; D004247:DNA; D003078:Colchicine