[Interaction of inorganic pyrophosphatase from yeast with alkylated derivatives of Sepharose].
The adsorption of inorganic pyrophosphatase from baker's yeast by alkylated polysaccharides involves both electrostatic and hydrophobic interactions. The enzyme is eluted by NaCl solutions of different ionic strengths depending on the adsorbent hydrophobicity. The degree of purification on different adsorbents varies from 2- to 80-fold.
D000478:Alkylation; D002850:Chromatography, Gel; D043564:Inorganic Pyrophosphatase; D011134:Polysaccharides; D011755:Pyrophosphatases / Q000302:isolation & purification* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012685:Sepharose / Q000031:analogs & derivatives*
D011134:Polysaccharides; D012685:Sepharose; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase
Vzaimodeĭstvie neorganicheskoĭ pirofosfatazy iz drozhzhet s alkilirovannymi proizvodnymi sefarozy.