Incorrect interpretations of radioactive labelling experiments with false transmitters.
In a number of recent studies attempts have been made to determine the subcellular origin of the released transmitter by comparison of the ratio of the radioactivity (14C/3H) of true and false transmitters between those released by stimulation and the subcellular fractions. It is shown that use of the isotopic or pseudo-molar ratios will lead to incorrect conclusions because varying degrees of isotopic dilution render the ratio meaningless. The only correct basis for comparison is the true molar ratio.
D005189:False Positive Reactions; D007553:Isotope Labeling; D018377:Neurotransmitter Agents / Q000378:metabolism; D011849:Radioactive Tracers
D018377:Neurotransmitter Agents; D011849:Radioactive Tracers