Humoral stimulation of hemopoietic progenitors from human fetal liver.
Hemopoietic cells from human fetal livers of 9-21 weeks gestation were cultured in semisolid media in vitro and in diffusion chambers implanted in mice. Myeloid colony formation in agar (CFU-C) was detected only in the presence of colony-stimulating factor. Formation of erythroid bursts in vitro was enhanced by addition of erythropoietin or erythroid-potentiating activity derived from a human T cell line (Mo). Fetal liver cells implanted in diffusion chambers gave rise to myeloid (CFU-DG) and occasionally to erythroid colonies. Administration of colony-stimulating factor derived from the T cell line to the host mice did not effect CFU-DG numbers. In contrast, a partially purified preparation containing erythroid-potentiating activity, caused a significant increase in the formation of CFU-DG. Similar stimulation was achieved by pretreatment of the diffusion chamber hosts with cyclophosphamide. None of these manipulations effected erythroid colony formation in diffusion chambers in mice.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003470:Culture Media; D003520:Cyclophosphamide / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology*; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology*; D051379:Mice; D013601:T-Lymphocytes / Q000502:physiology*
D003115:Colony-Stimulating Factors; D003470:Culture Media; D004921:Erythropoietin; D003520:Cyclophosphamide