Effect of homologous and heterologous interferons on proteolytic activity of normal and transformed cells.
The authors investigated the effect of various types of interferons (INF) on the activity of intracellular neutral and cysteine proteinases (cathepsin B and H) in normal and transformed rat embryonic fibroblasts. A substantially stronger inhibitory effect was observed with all three types of IFN on the proteolytic enzymes in transformed cells if compared to that on normal cells. Heterologous IFN reduces the activity of cysteine proteinases by 33% and neutral proteinases by 79%. Homologous IFN strongly reduced the activity of cysteine proteinases in transformed cells (59%) used in the experiments, but the inhibition of neutral proteinases remained nearly unchanged if compared with normal cells (18%).
D000818:Animals; D002401:Cathepsin B; D056655:Cathepsin H; D002403:Cathepsins / Q000037:antagonists & inhibitors; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D009361:Neoplasm Invasiveness; D051381:Rats
D007370:Interferon Type I; D007371:Interferon-gamma; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056655:Cathepsin H; C534128:Ctsh protein, rat