title: Regulation of nitrogen catabolic enzymes in Bacillus spp.
abstract: The levels of the inducible nitrogen catabolic enzymes arginase (L-arginine amidinohydrolase, EC 3.5.3.1) and alanine dehydrogenase (L-alanine:NAD+ oxidoreductase [deaminating], EC 1.4.1.1) from Bacillus licheniformis and histidase (L-histidine ammonia-lyase, EC 4.3.1.3) from Bacillus subtilis and the ammonia assimilatory enzymes from B. licheniformis were determined in cultures grown in the presence of different nitrogen sources. Although the levels of these enzymes were dependent upon the nitrogen source present, induction of the catabolic enzymes in response to the addition of inducer occurred even in the presence of preferred nitrogen sources. Intracellular pool sizes of ammonia, glutamate, glutamine, and alpha-ketoglutarate were measured in continuous cultures of b. licheniformis growing in the presence of different nitrogen sources. A comparison of the pool sizes of these metabolites with the ammonia assimilatory enzyme levels showed that the pools of the metabolites did not change in a manner consistent with their use as regulators of the synthesis of any of these enzymes.
mesh_terms: D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D001119:Arginase / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D001412:Bacillus subtilis / Q000201:enzymology*; D004790:Enzyme Induction; D004794:Enzyme Repression; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006638:Histidine Ammonia-Lyase / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*
chemical_list: D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D001119:Arginase; D006638:Histidine Ammonia-Lyase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen