Subcellular localization of immunoreactive dynorphin and vasopressin in rat pituitary and hypothalamus.
Dynorphin is found mainly in the particulate fraction of rat pituitary gland and hypothalamus homogenates. Dynorphin-like immunoreactivity (DYN-LI) from neurointermediate lobe (NIL) homogenates migrates at the same rate as vasopressin-like immunoreactivity (AVP-LI), in sucrose density gradients, whereas DYN-LI from the hypothalamus appears to migrate principally in a less dense region of the gradient. This suggests that dynorphin and vasopressin from pituitary are present in organelles of similar size and density, while the bulk of the dynorphin in the hypothalamus appears to be stored in a different subcellular organelle. Anterior lobe (AL) dynorphin appears to migrate in two separate bands on density gradients: the less dense band (slower) migrates at a similar rate to that of dynorphin and vasopressin from NIL. When alpha-neo-endorphin was measured in sucrose gradients of NIL and hypothalamus, it was found to co-migrate with DYN-LI.
D000818:Animals; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis*; D007031:Hypothalamus / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D010902:Pituitary Gland / Q000032:analysis* / Q000648:ultrastructure; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014667:Vasopressins / Q000032:analysis*
D004723:Endorphins; D014667:Vasopressins; D004399:Dynorphins