Histochemical studies on the distribution of thiamine pyrophosphatase and enzymes related to carbohydrate metabolism in the intercalated neurons of the rat supraoptic nucleus.
Histochemical studies have been conducted by applying hexokinase (HK), aldolase (AD), glyceraldehyde-3-phosphate dehydrogenase (G3), succinate dehydrogenase (SDH), glucose-6-phosphate dehydrogenase (G6PD), and thiamine pyrophosphatase (TPPase) methods, as well as Nissl staining and Gomori's chrome-alum-hematoxylin-phloxine (CHP) methods to intercalated neurons of the supraoptic nucleus (SO) on Wistar strain rats. Intercalated neurons reacted weakly to the AD, G3, G6PD, and SDH tests, indicating that they belong to the category of ordinary neurons with low carbohydrate metabolism. Many fibrous astrocytes showing strong HK reactions surround neurosecretory neurons. However, they do not surround intercalated neurons with mild HK activity. These results indicate that the latter receive a poor supply of energy from glucose in the circulating blood in contrast to the former. Intercalated neurons are very rich in Nissl substance but lack CHP-positive material. They may have a high potential for synthesizing protein. The principal morphological features of the TPPase-positive Golgi material are peculiar and heterogeneous shape and poor development. These findings together with mild G6PD activity suggest that intercalated neurons are very likely to have poor synthesizing activity.
D000818:Animals; D050260:Carbohydrate Metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006593:Hexokinase / Q000032:analysis; D006651:Histocytochemistry; D008297:Male; D009474:Neurons / Q000201:enzymology*; D009489:Neurosecretion; D011755:Pyrophosphatases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013495:Supraoptic Nucleus / Q000201:enzymology*; D013834:Thiamine Pyrophosphatase / Q000032:analysis*
D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase; D005634:Fructose-Bisphosphate Aldolase