title: Cross-linking of fibronectin to collagenous proteins.
abstract: Attempts were made to cross-link several collagenous proteins to fibronectin with Factor XIIIa (plasma transglutaminase). Cross-linking was demonstrated with type I collagen, type II collagen, type III collagen, type V or AB collagen, and alpha 1(I)-CB7 and alpha 1(I)-CB8 cyanogen bromide fragments of type I collagen. Cross-linking was not demonstrated with type IV collagen, Clq, and cyanogen bromide fragment alpha 1(I)-CB6. The pH optimum for cross-linking of alpha 1(I)-CB7 to fibronectin was 8.5 to 9.6. Cross-linking of alpha 1(I)-CB7 to fibronectin was somewhat enhanced at lower than physiological ionic strength.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D001777:Blood Coagulation; D003094:Collagen / Q000378:metabolism*; D003432:Cross-Linking Reagents; D005109:Extracellular Matrix / Q000378:metabolism; D005176:Factor XIII / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011503:Transglutaminases
chemical_list: D003432:Cross-Linking Reagents; D005353:Fibronectins; D003094:Collagen; D005176:Factor XIII; D000217:Acyltransferases; D011503:Transglutaminases