An improved method for the purification of "light" noradrenaline vesicles from rat vas deferens: some biochemical characteristics.
"Light" noradrenaline storage vesicles from nerve endings have been isolated by differential centrifugation and differential gradient centrifugation. They have been further purified by isopycnic sucrose/D2O centrifugation. By using these centrifugation techniques, we obtained an isopycnic gradient fraction in which noradrenaline was enriched about 41 times versus a total homogenate. This factor could be raised to 61 by using seminal ducts of castrated rats. Comparison of the distribution patterns in sucrose/D2O isopycnic gradients indicated that light noradrenaline vesicles of nerve endings contain Mg2+-stimulated ATPase and ATP, but that only a minor part of the dopamine beta-hydroxylase can be associated with these vesicles.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014649:Vas Deferens / Q000378:metabolism* / Q000648:ultrastructure
D004299:Dopamine beta-Hydroxylase; D008995:Monoamine Oxidase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D009638:Norepinephrine