'Superactivation' of alkaline phosphatase activity by cycloheximide in rat hepatoma cell cultures.
We have found that rat hepatoma cells (R-Y121B) retain alkaline phosphatase activity, and that this enzyme activity is increased by cycloheximide. Actinomycin D also increased the enzyme activity. This increase due to actinomycin D was partially inhibited by cycloheximide. The characteristics of alkaline phosphatase of the cells treated or untreated with cycloheximide or actinomycin D were similar to each other; they were heat labile and the enzyme reaction was strongly inhibited by L-homoarginine, but weakly by L-phenylalanine. The increase in alkaline phosphatase activity with cycloheximide has been termed a 'superactivation' of alkaline phosphatase.
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D051381:Rats
D003609:Dactinomycin; D003513:Cycloheximide; D000469:Alkaline Phosphatase