Expansion of cyclophosphamide-resistant cytotoxic precursors in vitro and in vivo by purified human interleukin 2.
Precursors of cytotoxic lymphoid cells obtained from mice treated with cyclophosphamide (CY) can be expanded in culture by alloantigens in the presence of purified human interleukin 2 (IL 2). Similarly, IL 2 delivered in vivo in a rate-controlled manner enhances cytotoxic activity in mice that are immunosuppressed by high doses of CY. The effector cells are Thy-1.2+ and are not elicited in the absence of antigen.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D003520:Cyclophosphamide / Q000494:pharmacology*; D004351:Drug Resistance; D007376:Interleukin-2 / Q000494:pharmacology*; D007519:Isoantigens / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007376:Interleukin-2; D007519:Isoantigens; D018800:Thy-1 Antigens; D003520:Cyclophosphamide