Dimethyl sulfoxide-induced transglutaminase activity in murine-derived Friend erythroleukemia cells.
Friend erythroleukemia cells (FL cells) derived from DBA/2 mice may be induced to differentiate in vitro by addition of dimethyl sulfoxide (DMSO) to the culture medium. Transglutaminase (glutaminyl-peptide gamma-glutamyltransferase, EC 2.3.2.13) (TGase) activity was detected in the lysates of uninduced FL cells and was markedly increased in DMSO-treated cells. DMSO induced TGase activity of differentiating FL cells in a dose-dependent manner over the concentration range 0-280 mM. The increase in TGase activity was observed after 1 day's cultivation of the cells with 280 mM DMSO and continued to 4 days. Another differentiation inducing agent, butyric acid (1.4 mM), was as effective as DMSO in enhancing TGase activity in FL cells. Treatment of controls, a lymphoma cell line and mouse erythrocytes, with DMSO or butyric acid had no effect. These results suggest that the increase in TGase activity in DMSO- or butyric acid-treated FL cells may be related to cell differentiation.
D000217:Acyltransferases / Q000096:biosynthesis*; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D005622:Friend murine leukemia virus; D006454:Hemoglobins / Q000096:biosynthesis; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D011503:Transglutaminases
D002087:Butyrates; D006454:Hemoglobins; D000217:Acyltransferases; D011503:Transglutaminases; D004121:Dimethyl Sulfoxide