Primary structures of three human pancreas peptides with growth hormone-releasing activity.
The primary structures of three polypeptides, possessing high intrinsic growth hormone-releasing activity and derived from a human pancreatic carcinoma which had caused acromegaly, were established by sequence analyses of the intact peptides and their cyanogen bromide digestion fragments with a gas-phase sequenator. The three human pancreas growth hormone-releasing factors contain 44 (hpGRF-44), 40 (hpGRF-40), and 37 (hpGRF-37) amino acids in identical sequences from their NH2 termini. High pressure liquid chromatography of the native peptides and their synthetic replicates showed that hpGRF-37 and hpGRF-40 possess free carboxyl termini while that of hpGRF-44 is amidated. The structure of hpGRF-44 was established as: Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly Ala-Arg-Ala-Arg-Leu-NH2.
D007516:Adenoma, Islet Cell / Q000032:analysis*; D000595:Amino Acid Sequence; D003488:Cyanogen Bromide; D006801:Humans; D007340:Insulinoma / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000302:isolation & purification*; D013004:Somatostatin
D010446:Peptide Fragments; D010455:Peptides; D013004:Somatostatin; D003488:Cyanogen Bromide