Standardization of the sucrose-potassium phosphate-glyoxylic acid histofluorescence method for tissue monoamines.
A modification of the sucrose-potassium phosphate-glyoxylic acid (SPG) histofluorescence method has resulted in consistent standardization of the fluorescent intensity while also eliminating a cumbersome step in the procedure. The present method results in highly rapid and sensitive visualization of monoaminergic neurons and their axonal varicosities in body tissue.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000033:anatomy & histology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D013696:Temperature
D018377:Neurotransmitter Agents