Tyrosine aminotransferase activity of frog (Rana esculenta) liver--II. Comparative aspects of intracellular distribution.
1. A subcellular fractionation procedure for frog liver is reported and validated by the distribution pattern of several marker enzymes, also in comparison with rat liver. 2. The subcellular distribution of tyrosine aminotransferase was investigated in frog liver as compared to rat liver: a different distribution of the enzyme was observed, being the activity mostly recovered in mitochondrial and cytosolic compartments. 3. Results indicate that mitochondrial tyrosine aminotransferase of both frog and rat liver is a matrix enzyme, even if differences are observed concerning its release from the organelles upon detergent treatment.
D000818:Animals; D002458:Cell Fractionation; D003840:Deoxycholic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011893:Rana esculenta; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014444:Tyrosine Transaminase / Q000378:metabolism*
D003840:Deoxycholic Acid; D014444:Tyrosine Transaminase