A new Salmonella tester strain, TA97, for the detection of frameshift mutagens. A run of cytosines as a mutational hot-spot.
We have developed a new Salmonella tester strain, TA97, for use in the Salmonella/microsome mutagenicity test. DNA sequencing has shown that this strain contains an added cytosine, resulting in a run of six cytosines at the mutated site in the histidine D gene. Its mutagenic specificity is similar to that of the frameshift mutagen tester strain, TA1537, which also contains an added cytosine in a run of cytosines and is currently among the five standard tester strains used for general mutagen screening. We assessed the mutagenic potency of 21 frameshift mutagens for TA1537 and TA97. TA97 was considerably more sensitive than TA1537 to reversion by these frameshift mutagens. In addition, one agent, PR toxin (from Penicillium roqueforti), which was not detected by any of the previously existing standard tester strains, did revert TA97; and two substituted aryl-alkyl triazenes, which had not been reported previously to be frameshift mutagens, were mutagenic in this new tester strain. We suggest that TA1537 be replaced by TA97 for general screening of mutagenicity.
D014150:Antipsychotic Agents / Q000494:pharmacology; D001483:Base Sequence; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000494:pharmacology; D003596:Cytosine; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D010640:Phenothiazines; D010957:Plasmids; D012486:Salmonella typhimurium / Q000187:drug effects; D013045:Species Specificity
D014150:Antipsychotic Agents; D001580:Benzopyrenes; D004269:DNA, Bacterial; D009153:Mutagens; D010640:Phenothiazines; D001564:Benzo(a)pyrene; D003596:Cytosine