Large-molecular-weight somatostatin in human adrenal medullary tissue.
Three human catecholamine-secreting adrenal medullary tumours, identified as phaeochromocytoma, were found to contain 774, 168, and 78 pmol/g of somatostatin-like immunoreactivity (SLI), compared to 40 pmol/g in a sample of normal human adrenal medulla. Sephadex-G50 gel-filtration chromatography of extracts from these tissues revealed SLI eluting in the position of somatostatin-14, somatostatin-28, and a peak eluting with a mol. wt. of about 5 K. After digestion of eluted material with clostridiopeptidase B, the predominant form of adrenal medullary SLI was found to elute in the position of a 7-K polypeptide.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism*; D002850:Chromatography, Gel; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D006801:Humans; D008970:Molecular Weight; D010673:Pheochromocytoma / Q000378:metabolism*; D013004:Somatostatin / Q000302:isolation & purification*
D013004:Somatostatin; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C019913:clostripain