The influence of pH and ionic strength on the single radial immunodiffusion test in qualitative assay of influenza virus haemagglutinin.
A significant dependence between different ionic strength and pH value of virus suspension on one hand and the haemagglutinin (HA) content as determined by single-radial-immunodiffusion (SRD) test on the other hand was observed after cleavage of influenza virus recombinant NIB-6 (H1N1) with sodium lauroyl sarcosinate (SLS). In contrast, no such relationship was found when the HA Of NIB-4 (H3N2) recombinant strain was determined.
D006389:Hemagglutinins, Viral / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D009994:Osmolar Concentration; D011995:Recombination, Genetic
D006389:Hemagglutinins, Viral