Preparation and properties of fluorescence labeled neuro- and cardiotoxin II from the sea anemone (Anemonia sulcata).
Labeling of the sea anemone toxin II with fluoresceinisothiocyanate is described. The native toxin and low molecular weight fluorescent compounds were completely separated from the derivative. Compared to fluorescein, the fluorescence of the toxin-derivative has a red shift and a decreased quantum yield. Like the native toxin, the fluorescent derivative affects the voltage-dependent sodium channel in nerve membranes. Under voltage-clamp conditions, the effect of the modified toxin on the inactivation of sodium channels was 45% of that of the unlabeled toxin.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003063:Cnidaria; D003064:Cnidarian Venoms / Q000032:analysis / Q000494:pharmacology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D007473:Ion Channels / Q000187:drug effects; D009413:Nerve Fibers, Myelinated / Q000187:drug effects; D009498:Neurotoxins / Q000494:pharmacology*; D012615:Sea Anemones; D012964:Sodium / Q000378:metabolism; D013861:Thiocyanates
D002316:Cardiotonic Agents; D003064:Cnidarian Venoms; D005452:Fluoresceins; D007473:Ion Channels; D009498:Neurotoxins; D013861:Thiocyanates; C028800:toxin II (Anemonia sulcata); D012964:Sodium; D016650:Fluorescein-5-isothiocyanate