title: Cyclic nucleotide phosphodiesterase activity in patients with obstructive airways disease.
abstract: Cyclic nucleotide phosphodiesterase activity was examined in the pulmonary tissues of patients with no obstructive airways disease, obstructive airways disease responsive to isoproterenol (responders) and obstructive airways disease non-responsive to isoproterenol (non-responders). The phosphodiesterase catalyzed hydrolysis of 0.45 microM levels of adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) was essentially the same in responders as in patients without disease, but was reduced in the non-responders. The results suggest that neither the postulated beta adrenergic receptor-cAMP system defect nor the depressed levels of cAMP reported in patients with bronchodilator-responsive obstruction is caused by an alteration of phosphodiesterase activity.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005541:Forced Expiratory Volume; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008168:Lung / Q000201:enzymology; D008173:Lung Diseases, Obstructive / Q000201:enzymology*
chemical_list: D000318:Adrenergic beta-Agonists; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D006152:Cyclic GMP; D007545:Isoproterenol