title: Influence of dietary status on liver palmitoyl-CoA hydrolase, peroxisomal enzymes, CoASH and long-chain acyl-CoA in rats.
abstract: In the livers of fasted rats, the activity of mitochondrial palmitoyl-CoA hydrolase was increased whereas the microsomal palmitoyl-CoA hydrolase activity decreased. Refeeding with a high-carbohydrate diet (glucose), the corresponding enzyme activities were decreased while refeeding with a high-fat diet (sheep tallow) increased the enzyme activities over the control values. The increased content of long-chain acyl-CoA and free CoASH under fasting and fat-refeeding was mainly attributed to the mitochondrial fraction with the remainder in the light mitochondrial fraction which contains peroxisomes. The results suggest a correlation of the compartmentation of the palmitoyl-CoA hydrolase and the content and compartmentation of the CoA derivatives in the liver under different nutritional states. The peroxisomal palmitoyl-CoA oxidase activity was increased by fasting. Fat-refeeding increased the activity even more; 1.8-fold as compared to the fasting animals. On the other hand, the activities of other peroxisomal enzymes which are not directly involved in the fatty acid metabolism such as urate oxidase were decreased to approximately the same extent by fasting. Re-feeding with glucose and fat further decreased the corresponding enzyme activity, particularly seen in the glucose-refed group.
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D003065:Coenzyme A / Q000378:metabolism*; D005215:Fasting; D005223:Fats / Q000008:administration & dosage; D005502:Food; D005947:Glucose / Q000008:administration & dosage; D008099:Liver / Q000201:enzymology*; D008297:Male; D008830:Microbodies / Q000201:enzymology; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D013869:Thiolester Hydrolases / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D005223:Fats; C013698:tallow; D013869:Thiolester Hydrolases; D010170:Palmitoyl-CoA Hydrolase; D005947:Glucose; D003065:Coenzyme A