Induction of gamma-glutamyl transpeptidase positive cells by benzo(a)pyrene in embryonic rat tongue cells in culture.
The utility of gamma-glutamyl transpeptidase as a histochemical marker to study the carcinogen induced changes in primary epithelial and fibroblasts mixed cell cultures of embryonic rat tongue by benzo(a)pyrene (BP) was explored. It was observed that at low BP concentrations selectively cells with fibroblasts morphology were intensely stained for GGT. At relatively higher concentrations of BP all the cells with fibroblasts morphology were eliminated and GGT positive areas were observed in cells with epithelial cell morphology. A carcinogen dose-related cell specificity for GGT activity was observed.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000494:pharmacology*; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006651:Histocytochemistry; D008954:Models, Biological; D051381:Rats; D014059:Tongue / Q000187:drug effects / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D001580:Benzopyrenes; D002273:Carcinogens; D001564:Benzo(a)pyrene; D005723:gamma-Glutamyltransferase