ATP citrate lyase in cholinergic nerve endings.
The activity of ATP-citrate lyase in homogenates of five selected rat brain regions varied from 2.93 to 6.90 nmol/min/mg of protein in the following order: cerebellum less than hippocampus less than parietal cortex less than striatum less than medulla oblongata and that of the choline acetyltransferase from 0.15 to 2.08 nmol/min/mg of protein in cerebellum less than parietal cortex less than hippocampus = medulla oblongata less than striatum. No substantial differences were found in regional activities of lactate dehydrogenase, pyruvate dehydrogenase, citrate synthase or acetyl-CoA synthase. High values of relative specific activities for both choline acetyltransferase and ATP-citrate lyase were found in synaptosomal and synaptoplasmic fractions from regions with a high content of cholinergic nerve endings. There are significant correlations between these two enzyme activities in general cytocol (S3), synaptosomal (B) and synaptoplasmic (Bs) fractions from the different regions (r = 0.92-0.99). These data indicate that activity of ATP-citrate lyase in cholinergic neurons is several times higher than that present in glial and noncholinergic neuronal cells.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000106:Acetate-CoA Ligase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002531:Cerebellum / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000201:enzymology*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003342:Corpus Striatum / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008526:Medulla Oblongata / Q000201:enzymology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000201:enzymology; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology
D009419:Nerve Tissue Proteins; D011768:Pyruvate Dehydrogenase Complex; D007770:L-Lactate Dehydrogenase; D002795:Choline O-Acetyltransferase; D002950:Citrate (si)-Synthase; D001275:ATP Citrate (pro-S)-Lyase; D000106:Acetate-CoA Ligase