title: Sympathetic effects on spontaneous activity in bovine mesenteric lymphatics.
abstract: To investigate the effects of autonomic nerves on the spontaneous activity, responses of valvular and intervalvular segments of isolated bovine mesenteric lymphatics to 60-strains of pulses (50 V for 0.5 ms at 2 Hz) have been studied under the influence of various drugs. There was a marked difference in response to transmural stimulation between the valvular and intervalvular segments. In the valvular segment, the stimulation arrested the spontaneous activity. However, the rhythm of spontaneous contractions was markedly accelerated during the stimulation in the intervalvular segment. Both inhibitory and excitatory effects were completely blocked by tetrodotoxin and guanethidine, potentiated by cocaine, and unaffected by hexamethonium and atropine. Propranolol blocked the inhibitory effect of the stimulation in the valvular segment. Pretreatment of the intervalvular segment with phentolamine converted the normal excitatory effect of the stimulation into an inhibitory influence. No change in rhythm was brought about by the stimulation in the presence of propranolol and phentolamine in either segment. These results suggest that transmural electrical stimulation causes the release of norepinephrine from postganglionic sympathetic nerve terminals in the vicinity of pacemaker cells and that beta-adrenoceptors are predominant in the valvular region of the lymph vessel.
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002417:Cattle; D003042:Cocaine / Q000494:pharmacology; D004553:Electric Conductivity / Q000187:drug effects; D004558:Electric Stimulation; D006145:Guanethidine / Q000494:pharmacology; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D008208:Lymphatic System / Q000187:drug effects / Q000294:innervation*; D009119:Muscle Contraction / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology*; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D006584:Hexamethonium Compounds; D018738:Hexamethonium; D013779:Tetrodotoxin; D001285:Atropine; D003042:Cocaine; D009638:Norepinephrine; D006145:Guanethidine