Microvessels from the spinal cord: isolation procedure and characterization of the fraction.
A simple method for the preparation of microvessels from the spinal cord is described. The fraction being enriched in the microvessels is characterized by morphological techniques and compared biochemically with the original suspension. The method makes possible detailed studies on the metabolism of microvessels and can be complementary to procedures that have been developed for separating the capillaries from other brain tissues.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002196:Capillaries / Q000648:ultrastructure; D006168:Guinea Pigs; D008833:Microcirculation / Q000201:enzymology / Q000648:ultrastructure; D008854:Microscopy, Electron; D013116:Spinal Cord / Q000098:blood supply*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase