Identification of the herpes simplex virus type 1 gene encoding the dUTPase.
The dUTPase gene of herpes simplex virus has been identified using a novel approach. The upstream regulatory sequences, or the promoter and upstream regulatory sequences, of the immediate-early gene Vmw175 (ICP4) were inserted in front of genes mapping in the region 0.69 to 0.70 map units of the virus genome to enhance transient gene expression in a transfection assay. One clone, containing a gene specifying a 1.5-kb mRNA, induced significant amounts of virus-specific dUTPase activity. The enzyme activity was abolished by insertion of a HindIII linker into the KpnI site within the coding sequences of this gene. The results show that the enzyme is virus coded and that 1.5-kb mRNA specifies the dUTPase.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D005796:Genes; D005809:Genes, Regulator; D005814:Genes, Viral; D007668:Kidney; D007700:Kinetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011755:Pyrophosphatases / Q000235:genetics*; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase