Does lead exposure influence liver protein synthesis in rats?
The liver and serum of rats was studied 18 hr following the i.p. administration of 0.5 mg Pb2+/100 g body wt. The incorporation of [14C]leucine into proteins was measured in ribosomal and mRNA fractions incubated in vitro and was markedly stimulated in the Pb2+ treated rats. Similar results were found when the proteins of liver and serum were labelled by the injection in vivo of [14C]leucine. The increased mRNA activity appears to be chiefly for acid serum proteins of approx. Mr 44,000, 41,000, 33,000 and 18,000.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007854:Lead / Q000633:toxicity*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012260:Ribonucleases / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D001798:Blood Proteins; D012333:RNA, Messenger; D007854:Lead; D012260:Ribonucleases