title: Trypanosoma brucei: a quick method for separating blood-stream trypomastigotes from infected blood by differential osmotic lysis.
abstract: 1. The degree of rat erythrocyte lysis and immobilization of Trypanosoma brucei in infected blood by buffered hypotonic solutions of sodium chloride and sources was studied. 2. At 0.3% sodium chloride solution 98% hemolysis of erythrocytes was achieved while 95% of the original bloodstream trypomastigotes survived and were found to be motile and viable for biochemical study. 3. Further increase in the concentration of sodium chloride above 0.3% revealed an increase in the immobilization of trypanosomes and a decrease in the erythrocyte hemolysis. 4. Bloodstream trypomastigotes have been prepared by differential osmotic lysis of infected blood in 0.3% sodium chloride solution and used for studying their metabolism.
mesh_terms: D000818:Animals; D006461:Hemolysis; D009994:Osmolar Concentration; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D014346:Trypanosoma brucei brucei / Q000302:isolation & purification* / Q000502:physiology