title: Transglutaminase and polyamine dependence of effector functions of human immunocompetent cells. The effect of specific inhibitors on lymphocyte proliferation and granulocyte chemiluminescence.
abstract: The effects of the transglutaminase inhibitor dansyl cadaverine (DC) and the polyamine antagonist methyl glyoxal-bis-(guanylhydrazone) (MeGbG) on the response of lymphocytes towards allogeneic and lectin stimulation and on the zymosan-induced chemiluminescence of neutrophilic granulocytes was studied. Application of DC resulted in dose-dependent suppression of chemiluminescence and lymphocyte proliferation; no difference of inhibitory potential occurred with variation of incubation time in the latter system. MeGbG was inactive in granulocytes, but inhibited lymphocyte proliferation; its effect increased with time. The experiments provide further evidence for the importance of transglutaminases and polyamines for the function of immunocompetent cells.
mesh_terms: D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D002103:Cadaverine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D003959:Diamines; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology*; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D049449:Luminescence; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008935:Mitoguazone / Q000494:pharmacology*; D011073:Polyamines / Q000378:metabolism*; D011503:Transglutaminases
chemical_list: D003959:Diamines; D006146:Guanidines; D011073:Polyamines; D000217:Acyltransferases; D011503:Transglutaminases; C008542:monodansylcadaverine; D002103:Cadaverine; D008935:Mitoguazone