Complexes of serum gamma-glutamyltransferase with apolipoproteins and immunoglobulin A.
We have detected complexes between gamma-glutamyltransferase and apolipoproteins or immunoglobulin A in sera from patients with hepatobiliary diseases but not in sera from healthy individuals. An average of 52.4% of the enzymic activity was precipitated by antiserum against apolipoprotein A, 29.9% by antiserum against apolipoprotein B, and 9.7% by antiserum against immunoglobulin A. Fifty to 60% of the enzyme activity was inhibited in the immunoprecipitates from the transferase fraction bound to apolipoprotein A or immunoglobulin A, and 21% in the fraction bound to apolipoprotein B. We identified the complexed transferase fractions by electrophoresis.
D000368:Aged; D001053:Apolipoproteins / Q000097:blood*; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D002780:Cholestasis, Intrahepatic / Q000201:enzymology; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006505:Hepatitis / Q000201:enzymology; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D008104:Liver Cirrhosis, Alcoholic / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D005723:gamma-Glutamyltransferase / Q000097:blood*
D001053:Apolipoproteins; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D007070:Immunoglobulin A; D005723:gamma-Glutamyltransferase