Fetal pulmonary maturity: relationship between optical density (650 nm) to the lecithin/sphingomyelin ratio and phosphatidylglycerol in amniotic fluid.
An optical density value greater than or equal to 0.15 at A650 n m of amniotic fluid obtained through transabdominal amniocentesis from a high risk obstetrical population, which excluded diabetes mellitus, isoimmunization, and amniotic fluid infection, correlated 98.6% with a mature lecithin to sphingomyelin ratio (L/S) greater than or equal to 2.0% and 41.6% with the presence of phosphatidylglycerol (PG). The corresponding false positive rates were 1.4% and 16.8%, respectively. In 30 infants delivered within 48 h of testing, A650 n m greater than or equal to 0.15 correlated 100% with absence of respiratory distress syndrome. A650 n m is not recommended for Rhesus immunized gestations, diabetic pregnancies and amniotic fluid infection due to a high false positive rate with A650 nm compared to L/S and PG.
D000653:Amniotic Fluid / Q000032:analysis / Q000502:physiology*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000503:physiopathology; D005325:Fetal Organ Maturity; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000196:embryology*; D010713:Phosphatidylcholines / Q000032:analysis*; D010715:Phosphatidylglycerols / Q000032:analysis*; D011247:Pregnancy; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis; D012189:Retrospective Studies; D013109:Sphingomyelins / Q000032:analysis*
D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D013109:Sphingomyelins