The effect of Ca2+ on the pharmacological specificity of [3H]glutamate binding sites in rat hippocampus.
The inhibition of [3H]glutamate binding to rat hippocampal membranes by various compounds was measured. L-Glutamic, L-2-amino-4-phosphonobutyric and 4-fluoroglutamic acids had similar IC50 values (2.12 microM, 4.98 microM and 3.90 microM respectively). D-Glutamate and D-2-amino-4-phosphonobutyrate were less potent than L-glutamate (IC50S 18.9 microM and 46.5 microM respectively) and L-glutamate diethylester (IC50 519 microM) was the weakest inhibitor tested. Addition of 10 mM CaCl2 caused a 2.27-fold increase in displaceable binding but did not alter the IC50 values of any of the inhibitors tested.
D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017470:Receptors, Glutamate
D005971:Glutamates; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D002118:Calcium