title: Triazolam protein binding and correlation with alpha-1 acid glycoprotein concentration.
abstract: On two occasions separated by a minimum of 1 wk, plasma was obtained from 12 patients (aged 18 to 73 yr) on dialysis after an overnight fast. Samples were assayed for albumin and alpha 1-acid glycoprotein (AGP) concentrations. 14C-Triazolam was added to each sample to a final concentration of 5 ng/ml. Protein binding was determined by equilibrium dialysis. Unbound triazolam ranged from 6.4% to 15.4% (mean = 10.0%). AGP concentrations ranged from 71.8 to 205.1 mg% (mean = 123.4 mg%). Triazolam binding ratio (bound/unbound concentration) correlated with AGP concentration (r2 = 0.69) but not with albumin concentration, age, or sex. This correlation was verified by adding AGP in varying amounts to control plasma.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000418:Albumins / Q000378:metabolism; D014151:Anti-Anxiety Agents / Q000378:metabolism*; D002250:Carbon Radioisotopes; D006801:Humans; D008875:Middle Aged; D009961:Orosomucoid / Q000378:metabolism*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011485:Protein Binding; D006435:Renal Dialysis; D014229:Triazolam / Q000097:blood / Q000378:metabolism*
chemical_list: D000418:Albumins; D014151:Anti-Anxiety Agents; D002250:Carbon Radioisotopes; D009961:Orosomucoid; D014229:Triazolam