title: Effects of autoimmune NGF deprivation in the adult rabbit and offspring.
abstract: An experimental autoimmune approach to the production of nerve growth factor deprivation, which we have previously described in the rat and guinea pig, has been applied to the rabbit. This species was chosen for study because of several potential advantages. The rabbit produces large litters and has a relatively short gestation period. More importantly, rabbits generate high titers of antibody against mouse NGF and large amounts of maternal antibody are passively transferred to the developing rabbit fetus compared to most other species, particularly the rat. The sympathetic nervous system of adult rabbit immunized against mouse NGF underwent degeneration with up to an 85% decrease in neuronal numbers in the superior cervical ganglion after 10 months of immunization, thus providing further evidence that NGF is required for the survival of mature sympathetic neurons. Despite the fact that newborn rabbits born to anti-NGF producing mothers had much higher titers of anti-NGF than did rats, the effects on the developing sympathetic and sensory nervous systems were not found to be any greater than in rats. Reductions in norepinephrine levels in the heart and spleen of adult rabbits born to anti-NGF producing mothers were greater than in small intestine. Prenatal exposure to maternal anti-NGF caused reductions (up to 70%) in the number of neurons in the dorsal root ganglia. Substance-P immunoreactivity was reduced in the substantia gelatinosa of the spinal cord of rabbit exposed to maternal anti-NGF. These changes, however, were not greater than seen in the rat. We conclude that although the rabbits offers some advantage in the study of the effects of NGF deprivation in the adult animal, it appears less well suited than the rat or guinea pig to the study of the effects of NGF deprivation on development.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D002490:Central Nervous System / Q000032:analysis; D003677:Deficiency Diseases / Q000201:enzymology / Q000276:immunology* / Q000503:physiopathology; D004837:Epinephrine / Q000032:analysis; D005260:Female; D008431:Maternal-Fetal Exchange; D008722:Methods; D009414:Nerve Growth Factors / Q000172:deficiency* / Q000276:immunology; D009638:Norepinephrine / Q000032:analysis; D011247:Pregnancy; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000503:physiopathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000906:Antibodies; D009414:Nerve Growth Factors; D014446:Tyrosine 3-Monooxygenase; D009638:Norepinephrine; D004837:Epinephrine