Pharmacological and physiological properties of benzodiazepine binding sites in rodent brown adipose tissue.
[3H]Diazepam and [3H] Ro5 -4864 were used as ligands to identify and characterize peripheral-type benzodiazepine binding sites in mouse and rat brown adipose tissue (BAT) membranes. [3H]Diazepam and [3H] Ro5 -4864 binding sites in BAT are pharmacologically similar to peripheral-type benzodiazepine binding sites in other tissues. Stimulators of central-type benzodiazepine receptors had no effect on or inhibited ligand binding to BAT membranes. Brown adipose tissue benzodiazepine binding sites are highly localized to mitochondria-containing subcellular fractions. These binding sites decrease with age in BAT from Fischer 344 rats. Stimulation of BAT thermogenesis in mice with 1-norepinephrine led to a decrease in [3H] Ro5 -4864 binding in the tissue.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism*; D001570:Benzodiazepinones / Q000378:metabolism; D001665:Binding Sites; D001831:Body Temperature; D003975:Diazepam / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A
D001570:Benzodiazepinones; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D001569:Benzodiazepines; C028513:4'-chlorodiazepam; D003975:Diazepam