Evidence for dynorphin-A as a neurotransmitter in rat hippocampus.
The molecular forms of Dynorphin A immunoreactivity (Dyn A-IR) detected in hippocampus were resolved by reverse-phase high pressure liquid chromatography (C18-HPLC). Peptide co-eluting with synthetic Dyn A(1-17) represents 20% of the total Dyn A-IR. Dyn A-IR was released from hippocampal slices in vitro by a Ca++-dependent mechanism stimulated by superfusion with K+, kainate or veratrine. Released peptides were resolved by C18-HPLC and shown to contain Dyn A-IR co-eluting with synthetic Dyn A(1-17). These observations are consistent with the hypothesis that Dyn A(1-17) acts as a neurotransmitter at synapses in hippocampus.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins; D004723:Endorphins / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014702:Veratrine / Q000494:pharmacology
D004723:Endorphins; D018377:Neurotransmitter Agents; D010446:Peptide Fragments; D004399:Dynorphins; D014702:Veratrine; D007608:Kainic Acid; D002118:Calcium