title: Characterization of progesterone-independent avidin production of chicken tissues in culture.
abstract: 1. Primary cell cultures of chick oviduct, intestine, peritoneal membrane and lung, but not those of wing muscle or calcaneal tendon, produced avidin progesterone-independently through a 5-week culture. 2. Actinomycin D and cycloheximide, but not hydroxyurea, inhibited the avidin production. 3. The common feature of the cultures producing avidin was a fibroblast-like cell type. 4. Similarly, oviduct and lung, but not muscle or tendon, also produced avidin in hormone-free short-term organ cultures, where the avidin synthesis began during the first 8 h of culture. 5. Induction of the general protein synthesis does not explain the avidin production, since another egg-white protein, ovalbumin, was not induced by the culture procedure.
mesh_terms: D000818:Animals; D001360:Avidin / Q000096:biosynthesis*; D002645:Chickens; D004054:Diethylstilbestrol / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques; D010047:Ovalbumin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D013710:Tendons / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001360:Avidin; D011374:Progesterone; D004054:Diethylstilbestrol; D010047:Ovalbumin