Effect of azelastine hydrochloride on macrophage chemotaxis and phagocytosis in vitro.
Azelastine, a newly synthesized anti-allergic agent, was tested for its effects on guinea pig macrophage chemotaxis and phagocytosis. As specific macrophage chemo-attractants, we used macrophage chemotactic factors a and c; separated and highly purified from inflamed skin sites. Macrophage chemotaxis induced by skin extract or chemotactic factors was significantly suppressed by a low concentration of the agent (1 microgram/ml); the effect was dose-dependent. The inhibition of chemotaxis was reversible, because chemotactic activity was restored when the agents was removed by washing cells before chemotactic assay. Inactivation of chemotactic factors was not detected by mixing azelastine and factors a and c. Azelastine may directly interact with macrophages to decrease their chemotactic responsiveness. beta-Glucuronidase activity in the medium and macrophages after phagocytosis of polystyrene latex particles was not affected by this agent at concentrations ranging from 1 to 10 micrograms/ml. The phagocytosis of latex particles or sheep red blood cells opsonized with IgG antibodies (EA) and anchoring of macrophages to substrate were not inhibited and azelastine did not damage the macrophages as determined by lactate dehydrogenase (LDH) release assay.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors; D002633:Chemotaxis / Q000187:drug effects*; D006168:Guinea Pigs; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000502:physiology; D008297:Male; D010587:Phagocytosis / Q000187:drug effects*; D010793:Phthalazines / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*
D002630:Chemotactic Factors; D006634:Histamine H1 Antagonists; D007166:Immunosuppressive Agents; D010793:Phthalazines; D011724:Pyridazines; C020976:azelastine