Optimalized conditions of tick-borne encephalitis virus production in vitro.
Conditions of the efficient production of tick-borne encephalitis (TBE) virus in chick embryo cell (CEC) monolayer cultures were investigated. The prerequisites of high recovery of the produced virus appeared to be: the use of dense cell monolayers, reasonable frequency of harvests and a suitable buffering of nutrient media. Using low input multiplicity of infection, the peak of the serum-free virus production was observed between 24 and 48 hr post infection (p.i.). The procedure elaborated was useful for isotopic labelling of the virus.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004669:Encephalitis Viruses, Tick-Borne / Q000032:analysis / Q000254:growth & development* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation; D014779:Virus Replication
D003470:Culture Media; D012367:RNA, Viral; D014764:Viral Proteins