Fusion of the lac genes to the promoter for the aminopeptidase N gene of Escherichia coli.
Strains of Escherichia coli K12 were isolated in which the lac structural genes were fused to the promoter for the aminopeptidasee N structural gene (pepN). Although this enzyme is constitutively produced, the differential rate of synthesis is increased about 4-fold upon phosphate starvation. The pepN-lac fusions were shown to respond to phosphate specific regulatory signals. A plaque forming lambda transducing phage bearing the pepN-lac fusion was isolated. This phage was used to prove genetically the fusion of lac genes to the promoter for the aminopeptidase. These results demonstrate a control at the transcriptional level of aminopeptidase synthesis.
D000626:Aminopeptidases / Q000235:genetics*; D010582:Bacteriophage lambda; D018826:CD13 Antigens; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D007763:Lac Operon; D009876:Operon; D010710:Phosphates / Q000172:deficiency; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D010710:Phosphates; D000626:Aminopeptidases; D018826:CD13 Antigens