Transglutaminase and polyamine dependence of effector functions of human immunocompetent cells. The effect of specific inhibitors on lymphocyte proliferation and granulocyte chemiluminescence.
The effects of the transglutaminase inhibitor dansyl cadaverine (DC) and the polyamine antagonist methyl glyoxal-bis-(guanylhydrazone) (MeGbG) on the response of lymphocytes towards allogeneic and lectin stimulation and on the zymosan-induced chemiluminescence of neutrophilic granulocytes was studied. Application of DC resulted in dose-dependent suppression of chemiluminescence and lymphocyte proliferation; no difference of inhibitory potential occurred with variation of incubation time in the latter system. MeGbG was inactive in granulocytes, but inhibited lymphocyte proliferation; its effect increased with time. The experiments provide further evidence for the importance of transglutaminases and polyamines for the function of immunocompetent cells.
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D002103:Cadaverine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D003959:Diamines; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology*; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D049449:Luminescence; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008935:Mitoguazone / Q000494:pharmacology*; D011073:Polyamines / Q000378:metabolism*; D011503:Transglutaminases
D003959:Diamines; D006146:Guanidines; D011073:Polyamines; D000217:Acyltransferases; D011503:Transglutaminases; C008542:monodansylcadaverine; D002103:Cadaverine; D008935:Mitoguazone