A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters.
Scrapie hamster brains contain at least 5-10 micrograms of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differential centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.
D000818:Animals; D001923:Brain Chemistry; D006224:Cricetinae; D008722:Methods; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D018622:PrP 27-30 Protein; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep
D009419:Nerve Tissue Proteins; D018622:PrP 27-30 Protein