Synthesis of delta-aminolaevulinate synthase in vitro using hepatic mRNA from chick embryos with induced porphyria.
Polyadenylated mRNA was isolated from chick embryo liver following induction of hepatic porphyria. The RNA was translated in vitro using a wheat germ cell-free system and delta-aminolaevulinate synthase was identified in the translation products by indirect immunoprecipitation. The enzyme was not apparent in the translation products of polyadenylated RNA from non-induced livers. The molecular weight of delta-aminolaevulinate synthase synthesized in vitro was 70000 and the protein was estimated to represent up to 5% of total products synthesised in vitro. These data demonstrate for the first time that induction of chick embryo liver delta-aminolaevulinate synthase activity in hepatic porphyria correlates with a large increase in the translational capacity of isolated polyadenylated RNA for this enzyme and, together with preliminary cDNA . RNA hybridization studies, indicate that an increase in the level of delta-aminolaevulinic synthase mRNA is responsible.
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002474:Cell-Free System; D056486:Chemical and Drug Induced Liver Injury; D002642:Chick Embryo; D004247:DNA; D008099:Liver / Q000201:enzymology*; D008107:Liver Diseases / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification / Q000502:physiology*; D011164:Porphyrias / Q000139:chemically induced / Q000201:enzymology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000502:physiology*
D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D000624:5-Aminolevulinate Synthetase