The interaction of Tetrahymena 30S dynein with bovine brain microtubules.
The binding of Tetrahymena 30S dynein to bovine brain microtubules has been examined. The dynein was bound by its B-end or ATP sensitive site with a stoichiometry of 1 dynein arm per 6 tubulin dimers. Addition of ATP released virtually all the dynein from the surface of the microtubule. Following hydrolysis of the ATP, the dynein reassociated with the microtubule.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002417:Cattle; D004398:Dyneins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D017239:Paclitaxel; D013768:Tetrahymena
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D004398:Dyneins; D017239:Paclitaxel