Expression of gamma-glutamyl transpeptidase in adult rat liver cells after transformation with SV40 virus.
By the use of histochemical techniques we have shown that the transformation of rat hepatocytes with simian virus 40 resulted in a large majority of cells producing gamma-glutamyl transpeptidase (gamma GT). Using a temperature-sensitive mutant of this virus we have shown that the percentage of cells exhibiting this enzyme in a temperature-sensitive SV40 mutant is much higher in cells grown at the permissive temperature of 33 degrees C than at the nonpermissive temperature of 40.5 degrees C. We thus conclude that, the correlation between gamma GT expression and chemical or spontaneous transformation of hepatocytes, previously described by other authors, can now be extended to viral transformation and that the enzyme activity is dependent on the expression of a transformed cell phenotype.
D000818:Animals; D002472:Cell Transformation, Viral; D002999:Clone Cells; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology*; D010641:Phenotype; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics; D013696:Temperature; D014776:Virus Cultivation; D005723:gamma-Glutamyltransferase / Q000032:analysis / Q000235:genetics*
D005723:gamma-Glutamyltransferase