Release of prostacyclin in vivo and its role in man.
Concentrations of 6-oxo-prostaglandin F1 alpha (6-oxo-PGF1 alpha), the stable hydrolysis product of prostacyclin (PGI2) were determined in venous blood sampled from a forearm vein after dextrose (10% w/v) perfusion and after subsequent distension with physiological saline in seven healthy volunteers. 6-oxo-PGF1 alpha was measured by gas chromatography/negative ion chemical ionisation mass spectrometry. Local release of PGI2 was demonstrated in six volunteers: in three both stimuli were effective and in three only one stimulus was effective. In seven trained athletes studied before and after vigorous exercise baseline plasma concentrations of 6-oxo-PGF1 alpha were less than 1.3-3.2 pg/ml. The concentration increased after exercise in each subject (mean 10.9 pg/ml; range 5.5-18.2 pg/ml). Mild chemical and mechanical stimuli therefore can cause local production of PGI2 from human blood vessels in vivo.
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000328:Adult; D001808:Blood Vessels / Q000378:metabolism; D002404:Catheterization; D002849:Chromatography, Gas; D011464:Epoprostenol / Q000096:biosynthesis / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D005082:Physical Exertion; D011453:Prostaglandins / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000378:metabolism; D013268:Stimulation, Chemical; D014680:Veins / Q000187:drug effects / Q000293:injuries
D011453:Prostaglandins; D011982:Receptors, Prostaglandin; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D005947:Glucose