title: Use of the fluorescent probe, 1-anilino-8-naphthalene sulfonate, to monitor the interaction of pesticide chemicals with mitochondrial membranes.
abstract: 1. The interaction of mitochondrial and submitochondrial membranes with DDT, methoxychlor and kelthane as monitored by fluorescence changes of ANS was studied. 2. The three organochlorine pesticide chemicals reduced the succinate- and ATP-dependent quenching of ANS fluorescence of mitochondria in a concentration-dependent manner. The results suggest that they blocked the supply of energy to the inner membrane. 3. The reversal of the substrate-induced quenching of ANS by uncouplers was abolished by DDT and kelthane probably as a consequence of the inhibition of protonophoric activity of the uncoupler. 4. Both DDT and kelthane were relatively ineffective inhibitors of substrate-induced quenching of ANS fluorescence of submitochondrial particles. The results suggest an asymmetry of the inner membrane with respect to DDT-inhibition of substrate-induced energization. 5. The possible basis of this asymmetry as well as of inhibition of energy supply to the membrane is discussed.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000817:Anilino Naphthalenesulfonates; D000818:Animals; D003634:DDT / Q000494:pharmacology; D004010:Dicofol / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010575:Pesticides / Q000494:pharmacology*; D011485:Protein Binding; D013386:Succinates / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D000817:Anilino Naphthalenesulfonates; D010575:Pesticides; D013386:Succinates; D014475:Uncoupling Agents; C027132:1-anilino-8-naphthalenesulfonate; D000255:Adenosine Triphosphate; D003634:DDT; D004010:Dicofol