title: Adenylate cyclase activity in bovine and human corneal endothelium.
abstract: Adenylate cyclase activity was measured in particulate fractions prepared from bovine and human corneal endothelium. Bovine enzyme activity was stimulated by fluoride, 5'-guanylylimidodiphosphate (GppNp) and beta-adrenergic agonists. Drug stimulation patterns of the human enzyme appeared qualitatively similar, but activities were generally higher than those of the bovine enzyme. This study demonstrates the existence of a receptor-mediated adenylate cyclase system in bovine and human corneal endothelium and suggests a possible role for cycli AMP in corneal endothelial physiology.
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D003315:Cornea / Q000187:drug effects / Q000201:enzymology*; D004727:Endothelium / Q000201:enzymology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006801:Humans
chemical_list: D000318:Adrenergic beta-Agonists; D006165:Guanylyl Imidodiphosphate; D000262:Adenylyl Cyclases