title: An experimental model to perform dynamic studies of exocrine pancreatic secretion in mice.
abstract: An experimental model to perform dynamic studies of exocrine pancreatic secretion from mice has been developed. It consists of a microsurgical procedure in anesthetized mice using a stereoscopic microscope. The bile-pancreatic common duct was individualized, isolated and cannulated. A specially calibrated capillary tube was placed in the free end of the cannula and put on a millimetrical rule. Readings of pure pancreatic juice flow rate were performed minute by minute to obtain a flow rate curve. The procedure allowed the discrimination among increasing betanechol doses of 0.1, 0.2, and 0.4 microgram/g body weight.
mesh_terms: D000818:Animals; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology*; D002404:Catheterization; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008954:Models, Biological; D010189:Pancreatic Juice / Q000378:metabolism*
chemical_list: D001626:Bethanechol Compounds; D018723:Bethanechol