An in-vivo method for estimating inhibin production by adult rat testes.
The concentrations of inhibin in samples of rat testicular venous and arterial blood and interstitial fluid were measured by an in-vitro bioassay using pituitary cells in culture in which the standard was an ovine testicular lymph preparation (assigned potency 1 unit/mg). Inhibin levels were undetectable (less than 2 U/ml) in both blood samples but reached a mean concentration of 120 +/- 7 U/ml in testicular interstitial fluid. After unilateral efferent duct ligation the rate of inhibin accumulation in seminiferous tubules was determined by the difference in the inhibin content of the ligated and unligated testes. Additionally, the rate of seminiferous tubule fluid production was obtained from the difference in weight between the ligated and non-ligated testes. In the 24 h after efferent duct ligation there were linear increases in inhibin (18.5 +/- 1.0 U/h) and in seminiferous tubule fluid production (26 +/- 1 microliter/h), but there were no changes in serum FSH and LH levels. Experimental induction of bilateral cryptorchidism led to a decrease in the inhibin content of the testis after 10 days. The rate of inhibin accumulation after efferent duct ligation declined more rapidly than the inhibin content, being significantly depressed in cryptorchid testes after 3 days, suggesting that this measurement is a more sensitive index of inhibin production than the determination of testicular inhibin content.
D000818:Animals; D002478:Cells, Cultured; D003456:Cryptorchidism / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D007265:Inhibins / Q000097:blood / Q000378:metabolism*; D008026:Ligation; D008297:Male; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000378:metabolism; D013737:Testis / Q000098:blood supply / Q000378:metabolism*; D014680:Veins
D007265:Inhibins