title: Effect of food deprivation on rat gastric somatostatin and gastrin release.
abstract: The influence of food deprivation on the release of somatostatin and gastrin from the rat stomach was investigated using an isolated, vascularly perfused rat stomach preparation. Basal and acetylcholine-stimulated gastrin release were significantly lower after a 3 day starvation, whereas the inhibitory effect of acetylcholine and the stimulatory effect of glucagon on somatostatin secretion were not influenced by fasting. In dose-response studies, isoproterenol dose-dependently stimulated somatostatin secretion. The increases were similar in both groups fasted for 12 and 72 h. Gastrin release remained at basal levels. Bombesin dose-dependently increased gastrin secretion; this stimulatory effect on the G cell was significantly reduced after a 72-h starvation. Somatostatin secretion was only weakly stimulated by high concentration of bombesin revealing no effect of fasting. Somatostatin content of the nonperfused stomach declined from 57 +/- 4 pmol/stomach in fed controls to 36 +/- 3 pmol/stomach after a 72-h fast. Antral gastrin concentration decreased by 42% in a 3-day fasting period. It is concluded that rat gastric somatostatin release in vitro is--in contrast to gastrin--not altered by food deprivation while the somatostatin content in gastric tissue declined during fasting.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005508:Food Deprivation / Q000502:physiology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D005934:Glucagon / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D005755:Gastrins; D013004:Somatostatin; D005934:Glucagon; D007545:Isoproterenol; D000109:Acetylcholine; D001839:Bombesin