Detection of TSH-binding inhibitor immunoglobulins by using the triton-solubilized receptor from human thyroid membranes.
A radioreceptor assay of TSH using Triton-solubilized human thyroid receptors was applied to the detection of TSH-binding inhibitor immunoglobulins (TBII). In 26 untreated patients with Graves' disease, 20 (76.9%) were found positive in this assay, while 18 (69.2%) cases were found positive by the conventional assay using human thyroid particulate receptors. In 25 patients with goitrous Hashimoto's thyroiditis, 2 (8.0%) were found positive by the assay using soluble receptor, while 3 (12.0%) were found positive by the conventional one. A significant correlation was found between TBII activities in the patients with untreated Graves' disease detected by these two assay systems (r=0.69; n=26; p less than 0.001). These data support the concept that TBII are antibodies against the TSH receptor or its closely related structures.
D002462:Cell Membrane / Q000032:analysis; D006111:Graves Disease / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D018828:Immunoglobulins, Thyroid-Stimulating; D011092:Polyethylene Glycols; D011869:Radioligand Assay / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism; D011989:Receptors, Thyrotropin; D013961:Thyroid Gland / Q000032:analysis; D013967:Thyroiditis, Autoimmune / Q000276:immunology; D013972:Thyrotropin / Q000378:metabolism
D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D011956:Receptors, Cell Surface; D011989:Receptors, Thyrotropin; D011092:Polyethylene Glycols; D013972:Thyrotropin