Selective effect of the metallocarcinogen beryllium on hormonal regulation of gene expression in cultured cells.
Effects of the metallocarcinogen beryllium on regulation of gene expression were assessed by analysis of hormonal regulation of synthesis of tyrosine aminotransferase in beryllium-treated hepatoma cell cultures. Cell growth was not affected by exposure of the cells to 1 micro M BeSO4 throughout their 4- to 5-day growth cycle. In cells pretreated in this way, the induction by glucocorticoids was specifically impaired, the extent of induced enzyme synthesis being reduced about 50%. Inductions by insulin or cyclic adenosine 3':5'-monophosphate were not influenced by the metal. The results suggest that low concentrations of beryllium selectively interfere with regulatory mechanisms controlling transcriptional events in gene expression.
D000818:Animals; D001608:Beryllium / Q000494:pharmacology*; D002273:Carcinogens; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D051381:Rats; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014444:Tyrosine Transaminase / Q000096:biosynthesis* / Q000235:genetics
D002273:Carcinogens; D007328:Insulin; D000242:Cyclic AMP; D014444:Tyrosine Transaminase; D001608:Beryllium; D006854:Hydrocortisone