Mixed-function oxidase studies in the redfish, Sciaenops ocellata, from Galveston Bay, Texas.
The metabolism of parathion to para-nitrophenol (PNP) in redfish (Sciaenops ocellata) liver microsomes has been both identified and characterized. This mixed-function oxidase (MFO) reaction in redfish requires NADPH and is inhibited by carbon monoxide. It exhibits a temperature optimum of 25 degrees C but no clear pH optimum between 7.0-8.5. Redfish hepatic microsomal MFOs were not induced by 2.5 days after a single i.p. injection of 88 mg Aroclor 1254/kg body wt, but under the same dosage and time conditions male albino Swiss mice were significantly induced (p less than 0.05).
D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001711:Biotransformation; D020111:Chlorodiphenyl (54% Chlorine); D004790:Enzyme Induction; D005399:Fishes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism; D010278:Parathion / Q000378:metabolism; D013045:Species Specificity; D013696:Temperature; D013781:Texas
D001140:Aroclors; D009596:Nitrophenols; D020111:Chlorodiphenyl (54% Chlorine); D010278:Parathion; D006899:Mixed Function Oxygenases; C024836:4-nitrophenol