Neurotransmitters extraction by local intracerebral dialysis in anesthetized rats.
A new technique for in vivo neurotransmitter extraction was developed. Perfusion U shaped cannulae were chronically implanted in the caudate or the thalamus of rats. A segment of the cannula was a piece of dialysis tube of molecular weight cut off 1000. Measurable amounts of dopamine and norepinephrine were recovered from the perfusion fluid. Intraperitoneal amphetamine 10 mg/kg increased dopamine output from the caudate but not from the thalamus. The potential applications of this technique are discussed.
D000758:Anesthesia; D000818:Animals; D001923:Brain Chemistry; D002421:Caudate Nucleus / Q000378:metabolism; D003956:Dialysis; D004298:Dopamine / Q000378:metabolism; D008297:Male; D018377:Neurotransmitter Agents / Q000302:isolation & purification*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013788:Thalamus / Q000378:metabolism
D018377:Neurotransmitter Agents; D004298:Dopamine; D009638:Norepinephrine