Establishment of a mosquito cell line from Haemagogus equinus larvae.
The establishment and characterization of a Haemagogus equinus mosquito cell line (GML-HE-12) are described. The cells are diploid (2N = 6) and seem to be free of contaminants. Their susceptibility to 13 arboviruses was tested. Eleven of the viruses multiplied in this cell line; six of these viruses still showed titers of 4 log10 plaque forming unit/ml or greater at 33 d postinoculation. No overt cytopathologic effect was observed.
D000818:Animals; D001103:Arboviruses / Q000254:growth & development*; D002455:Cell Division; D002460:Cell Line; D009033:Culicidae / Q000166:cytology* / Q000201:enzymology / Q000382:microbiology; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D007527:Isoenzymes / Q000378:metabolism; D007621:Karyotyping; D007814:Larva / Q000166:cytology; D010948:Viral Plaque Assay
D003470:Culture Media; D007527:Isoenzymes