The stoichiometry of the Ca2+-pumping ATPase of erythrocytes.
The stoichiometry of the erythrocyte Mg2+ dependent Ca2+-stimulated ATPase has been determined in a reconstituted system. Purified Ca2+ ATPase was incorporated into calcium impermeable liposomes and the ATP dependent calcium uptake was determined simultaneously with the hydrolysis of ATP. The results indicate that 1 gram atom of calcium is transported for each gram molecule of ATP hydrolysed, i.e., an ATP/Ca2+-stoichiometry of 1.
D000255:Adenosine Triphosphate / Q000378:metabolism; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases / Q000097:blood* / Q000302:isolation & purification; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics
D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases