title: Comparison of indirect double antibody and double antibody sandwich ELISA techniques with latex agglutination test for the diagnosis of human rotavirus infection.
abstract: 94 faecal samples from infants and children suffering of acute gastroenteritis were investigated for rotavirus by indirect double antibody sandwich ELISA kit (WHO, Geneva), Rotavirus ELISA kit (DAKOPATTS A/S, Copenhagen) and Rotalex latex-agglutination kit (Orion Diagnostica, Helsinki). The ELISA techniques gave almost identical results and seemed to be of same sensitivity and specificity. Rotalex agglutination had an overall agreement of 88% with ELISA. It is concluded that strongly positive reactions found by Rotalex may be regarded as true positive reactions, whereas samples producing weakly positive and/or negative reactions should be retested in a more specific and sensitive assay, such as enzyme linked immunosorbent-assay (ELISA).
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D007158:Immunologic Techniques; D007841:Latex Fixation Tests; D012401:Rotavirus / Q000276:immunology; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000276:immunology