Reappearance of beta-adrenergic receptors after isoproterenol treatment in intact C6-cells.
The reappearance of beta-adrenergic receptors in C6-glioma cells after desensitization with isoproterenol was studied using the antagonist [3H]CGP-12177. Reappearance had the following properties: (a) it occurred in intact cells only, (b) it was temperature dependent, (c) it required an Na+/H+ gradient, low intracellular Ca2+ activity, and (d) it required ATP, and (e) intact lysosomes. The results suggest endocytosis and recycling of the beta-adrenergic receptor after agonist treatment.
D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000818:Animals; D002412:Cations / Q000494:pharmacology; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D011412:Propanolamines / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D013696:Temperature
D000319:Adrenergic beta-Antagonists; D002412:Cations; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D003513:Cycloheximide; D007545:Isoproterenol; C033394:CGP 12177