Antibodies to the calmodulin-binding Ca2+-transport ATPase from smooth muscle.
Antibodies were raised against a calmodulin-binding CaMg-ATPase (Ca2+-transport ATPase) from smooth muscle. The binding of these antibodies to a number of related Ca2+-transport ATPases was studied. Antibodies to the calmodulin-binding ATPase from porcine antrum (stomach) smooth muscle do not only bind to this CaMg-ATPase, but also to the corresponding enzyme in porcine erythrocytes. However, they do not bind to the CaMg-ATPase from sarcoplasmic reticulum of porcine skeletal muscle. The binding of these antibodies to the CaMg-ATPase of smooth muscle, does not inhibit the enzyme activity.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001692:Biological Transport; D017301:Ca(2+) Mg(2+)-ATPase; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000276:immunology*; D002147:Calmodulin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009130:Muscle, Smooth / Q000201:enzymology*; D011485:Protein Binding; D013270:Stomach / Q000201:enzymology; D013552:Swine
D000906:Antibodies; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases