Effect of 3'-methyl-4-dimethylaminoazobenzene on liver cells from adult rat in primary culture.
Primary mass cultures of isolated liver cells, which prepared from normal adult rat by a collagenase-liver-perfusion technique, were treated with 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) to study the production of transformed liver cells. Enzymatically isolated liver cells had high sensitivity to 3'-Me-DAB in primary culture. By 2- or 6-day treatment, mature hepatocytes remarkably decreased in their numbers due to cytotoxic effect of 3-Me-DAB, but thereafter active proliferation of epithelial-like clear cells was observed. Six-day treatment induced epithelial-like clear cells with gross chromosomal abnormalities, although 2-day treatment failed to induce transformed cells. However, the transformed epithelial-like clear cells with chromosomal abnormalities were negative for gamma-glutamyl-transpeptidase (GGT).
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003593:Cytoplasm / Q000648:ultrastructure; D007621:Karyotyping; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008749:Methyldimethylaminoazobenzene / Q000494:pharmacology*; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene; D005723:gamma-Glutamyltransferase