Enhancement of long-chain acyl-CoA hydrolase activity in peroxisomes and mitochondria of rat liver by peroxisomal proliferators.
The present study has confirmed previous findings of long-chain acyl-CoA hydrolase activities in the mitochondrial and microsomal fractions of the normal rat liver. In addition, experimental evidence is presented in support of a peroxisomal localization of long-chain acyl-CoA hydrolase activity. (a) Analytical differential centrifugation of homogenates from normal rat liver revealed that this activity (using palmitoyl-CoA as the substrate) was also present in a population of particles with an average sedimentation coefficient of 6740 S, characteristic of peroxisomal marker enzymes. (b) The subcellular distribution of the hydrolase activity was greatly affected by administration of the peroxisomal proliferators clofibrate and tiadenol. The specific activity was enhanced in the mitochondrial fraction and in a population of particles with an average sedimentation coefficient of 4400 S, characteristic of peroxisomal marker enzymes. Three populations of particles containing lysosomal marker enzymes were found by analytical differential centrifugation, both in normal and clofibrate-treated rats. Our data do not support the proposal that palmitoyl-CoA hydrolase and acid phosphatase belong to the same subcellular particles. In livers from rats treated with peroxisomal proliferators, the specific activity of palmitoyl-CoA hydrolase was also enhanced in the particle-free supernatant. Evidence is presented that this activity at least in part, is related to the peroxisomal proliferation.
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D002500:Centrifugation, Isopycnic; D002994:Clofibrate / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D013869:Thiolester Hydrolases / Q000378:metabolism*
D005233:Fatty Alcohols; C004568:tiadenol; D013869:Thiolester Hydrolases; D010170:Palmitoyl-CoA Hydrolase; D002994:Clofibrate