title: Lysosomal damage under nitrite intoxication in rainbow trout (Salmo gairdneri Rich.).
abstract: 1. In Salmo gairdneri specimens exposed to 450 micrograms/l NO2-N (nearly 36 hr LC50) for various exposure times (12-72 hr) some parameters of liver lysosomal function were examined. 2. In vivo both total proteolytic activity and single protease activities were inhibited, the inhibition increasing with the lengthening of exposure time. In vitro no analogous effect was observed. 3. Lysosomal membranes showed an increased fragility correlated with the physiological conditions of treated animals. 4. Data were considered as the results of a nitrite mediated effect on functional and structural proteins.
mesh_terms: D000818:Animals; D002401:Cathepsin B; D002403:Cathepsins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D009573:Nitrites / Q000506:poisoning*; D010447:Peptide Hydrolases / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D013997:Time Factors; D014337:Trout / Q000378:metabolism*
chemical_list: D009573:Nitrites; D002403:Cathepsins; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D002401:Cathepsin B