Two distinct human cloned T cell lines that exhibit natural killer-like and anti-human effector activities.
Cloned T cell lines from mixed lymphocyte cultures stimulated with autologous Epstein Barr virus- (EBV) transformed lymphoblastoid cell line (LCL) cells were established using a limiting dilution technique in the presence of T cell growth factor (TCGF). The T cell lines included two distinct clones of cytotoxic T cells (Tc) in addition to EBV-specific Tc. A cytotoxic profile of one cloned line was similar to that of endogenous NK cells in peripheral blood. The other cloned Tc line showed an anti-human cytotoxicity. The susceptible targets for this latter Tc line were various human cells, including autologous LCL and peripheral blood lymphocytes (PBL), stimulated with pokeweed mitogen, along with NK-sensitive and NK-resistant cell lines. Weak cytotoxic activity was detected against various xenogeneic cell lines. Furthermore, autologous and allogeneic cloned T cell lines were resistant to killing by the anti-human effector clone. These t wo distinct cloned Tc lines expressed the Leu-1 and Leu-2a antigens, which are markers of suppressor/cytotoxic T cells.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002143:Callitrichinae; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007376:Interleukin-2; D008565:Membrane Proteins; D018800:Thy-1 Antigens