Suppression by adenine compounds of histamine-induced contraction in isolated guinea pig tracheal rings.
The effects of purine compounds on the contraction of isolated guinea pig tracheal pigs induced by histamine were studied in vitro. Adenosine, at 0.1-1 mM concentrations, suppressed the contraction induced by histamine in the isolated guinea pig tracheal rings in a concentration-dependent manner. ATP, AMP and adenine were also effective in blocking tracheal constriction induced by histamine. The other purine compounds such as inosine, guanosine, guanine and hypoxanthine had no effect on tracheal smooth muscle. Coformycin, which is a competitive inhibitor of adenosine deaminase, did not potentiate adenosine action. Dipyridamole, which blocks adenosine uptake, did not impair the suppression caused by adenosine. These results suggest that adenosine action on tracheal smooth muscle cells may be mediated by direct action on cell surface receptors. In addition, it seems that adenosine is an important modulator of tracheal smooth muscle cell function.
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003070:Coformycin / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D006168:Guinea Pigs; D006634:Histamine H1 Antagonists; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects
D006634:Histamine H1 Antagonists; D003070:Coformycin; D000249:Adenosine Monophosphate; D004176:Dipyridamole; D000255:Adenosine Triphosphate; D000225:Adenine