title: Stimulation of glycolysis by placental polypeptides and inhibition by duramycin.
abstract: Placental polypeptides present in crude preparations of transforming growth factors stimulate glycolysis when added to quiescent 3T3 cells, normal rat kidney, and chick embryo fibroblasts. The stimulation was apparent over a time period of at least 90 min and was seen at glucose concentrations ranging from 1 to 30 mM. Duramycin, an antibiotic isolated from Streptomyces cinnamomeus, inhibits the polypeptide-stimulated and nonstimulated glycolysis of intact cells, since it permeabilizes cells to Pi and nucleotides. However, duramycin also inhibits the Na+-K+-ATPase as well as the ouabain-insensitive Mg2+-ATPase of plasma membranes. Duramycin has no effect on glycolysis catalyzed by cell-free extracts of Ehrlich ascites tumor cells in the presence of mitochondrial ATPase but partially inhibits glycolysis when ADP and Pi are generated by ATPases of plasma membrane preparations.
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D017301:Ca(2+) Mg(2+)-ATPase; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D004285:Dogs; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007679:Kidney Medulla / Q000201:enzymology; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D051379:Mice; D008861:Microsomes / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D006133:Growth Substances; D007773:Lactates; D010455:Peptides; D011257:Pregnancy Proteins; C040522:duramycin; D019344:Lactic Acid; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase