Restricted replication of herpes simplex virus type 1 in pig kidney cells.
Pig kidney (PK) cells infected with herpes simplex virus type 1 (HSV-1) strains HSZP and RO, respectively, synthesized low amounts of infectious virus; virus-specific antigens detected by immunofluorescence (IF) staining were seen only in a small proportion of cells. When propagated in 2-4 subsequent passages, PK cells infected with the HSZP strain (PK-HSZP cells) became free of infectious virus and virus-specific antigens as detected by IF staining. In contrast, PK cells infected with the RO strain (PK-RO cells) permanently harboured the infectious virus and virus-specific antigens in the course of 10 subsequent passages. The latter were detectable 48-72 hr after seeding. The constantly low virus yields were significantly enhanced when restrictive PK cells were mixed with permissive ZP cells. Cocultivation of variable amount of PK cells (at the 2nd, 5th and 7th passages) with a constant number of ZP cells showed that 10(2)-10(3) PK cells were necessary to transfer the HSV to indicator cells. Immediate early and early HSV-1 coded antigens were seen by IF in the nuclei of a small proportion of PK-RO cells; similarly, virion and nucleocapsid antigens were found in the nuclei and cytoplasm of a few cells of the PK-RO monolayers.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007668:Kidney; D008168:Lung; D011817:Rabbits; D018139:Simplexvirus / Q000254:growth & development* / Q000276:immunology; D013552:Swine; D014779:Virus Replication
D000956:Antigens, Viral