Purification of radioiodinated somatostatin-related peptides by reversed-phase high-performance liquid chromatography.
In order to set up specific radioimmunoassays for the two N- and C-terminal tetradecapeptides of somatostatin-28 the peptides somatostatin SS14 and SS28 and the somatostatin by-products 1-Tyr-SS14, 11-Tyr-14 and desaminotyrosyl-beta-alanine fragment (1----14) of SS28 were radioiodinated by the chloramine-T or Bolton-Hunter techniques. Reversed-phase high-performance liquid chromatography was shown to be a very efficient and reliable method for the purification of different radioactive reaction media. The corresponding labelled peptides were tested for their relative immunological (radioimmunoassay) and biological (binding studies) properties.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D010179:Pancreas / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification; D011863:Radioimmunoassay; D011956:Receptors, Cell Surface / Q000032:analysis; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000302:isolation & purification* / Q000378:metabolism; D014443:Tyrosine / Q000032:analysis
D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D017481:Receptors, Somatostatin; D014443:Tyrosine; D013004:Somatostatin