title: Biosynthetic regulation of the human transferrin receptor by desferrioxamine in K562 cells.
abstract: Treatment of K562 cells with the iron chelator desferrioxamine results in the gradual increase in total cell receptors for transferrin. Receptor number rises 2.5-4.5-fold over 24 h and remains at the elevated level if the chelator is continuously present. Preincubation of the chelator with ferric chloride abolishes the effect. The drug has no effect on the 7-h half-life of the receptor. The increased number of receptors can be accounted for by a specific increase in the rate of receptor biosynthesis which reaches 3-4 times that seen in untreated cells by 6 h after the addition of the chelator. Isolation of mRNA from treated cells reveals that, after 8 h in the presence of desferrioxamine, there is a 3-fold increase in the specific translation of transferrin receptor over untreated cells. Total protein synthesis is not changed under these conditions.
mesh_terms: D000818:Animals; D002460:Cell Line; D003676:Deferoxamine / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011990:Receptors, Transferrin; D012156:Reticulocytes / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin; D011061:Poly A; D003676:Deferoxamine