Attenuated ts-recombinants of influenza A/USSR/77 (H1N1) virus obtained by crossing with the cold-adapted donor A/Leningrad/134/57 (H2N2) virus.
Conditions of obtaining attenuated influenza virus recombinants by crossing of a cold-adapted donor with A (H1N1) influenza virus that reappeared in 1977 were studied. Temperature-sensitive recombinants suitable for intranasal immunization of adults with low titres of anti-haemagglutinin and anti-neuraminidase antibodies, and possessing sufficiently high immunogenicity were obtained by crossing of native parent strains and cross-reactivation techniques. It was confirmed that the cold-adapted A/Leningrad/134/17/57 (H2N2) influenza virus variant is a prospective donor of attenuation for obtaining recombinants--candidates for live influenza vaccine strains.
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D007114:Immunization; D053118:Influenza A Virus, H1N1 Subtype; D053121:Influenza A Virus, H2N2 Subtype; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology; D009439:Neuraminidase / Q000276:immunology; D011995:Recombination, Genetic; D013696:Temperature
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D009439:Neuraminidase