title: [Na+, K+ -ATPase: causes of activation of K+ -phosphatase reaction by nucleotides].
abstract: Hydrolysis of umbelliferone phosphate is inhibited by sodium ions and ATP added to the incubation medium together with Na+ activates it, CTP, ITP, UTP and GTP produce the same effect. Acetyl phosphate, p-nitrophenyl phosphate, ADP and adenylyl imidodiphosphate beta, gamma-NH-ATP, ATP nonhydrolyzable analog, have no activating effect. ATP produces a mixed inhibition of umbelliferone phosphate hydrolysis both in the presence and absence of Na+. A scheme is suggested which explains the mechanism of the nucleotide activating effect.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000495:Allosteric Site; D000818:Animals; D027682:Cation Transport Proteins; D003570:Cytidine Triphosphate / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000378:metabolism*; D004372:Ducks; D004789:Enzyme Activation; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008968:Molecular Conformation; D010710:Phosphates; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013379:Substrate Specificity; D014468:Umbelliferones / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D003597:Cytosine Nucleotides; D010710:Phosphates; D014468:Umbelliferones; D003570:Cytidine Triphosphate; D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; C022761:potassium transporting ATPase; D000254:Sodium-Potassium-Exchanging ATPase
vernacular_title: Na+, K+ -ATPaza: prichiny aktivatsii K+ -fosfataznoĭ reaktsii nukleotidami.