A new sensitive, thiol tolerant method for the determination of inorganic pyrophosphatase.
In this work a new sensitive, thiol tolerant method for the determination of inorganic pyrophosphatase (EC 3.6.1.1) was developed. It is based on the formation of phosphomolybdate-triethylamine complex, which in acid-acetone mixture forms a white, homogeneous precipitate, the turbidity of which can accurately measured spectrophotometrically at 355 nm. This method can be easily applied to all the Pi-producing enzymes whose substrates are stable enough in our acidic experimental conditions.
D004355:Drug Stability; D013293:Enterococcus faecalis / Q000201:enzymology; D007202:Indicators and Reagents; D043564:Inorganic Pyrophosphatase; D007700:Kinetics; D011755:Pyrophosphatases / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013438:Sulfhydryl Compounds
D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase