Analysis of oxprenolol in formulations by high-performance liquid chromatography.
A simple, accurate, and rapid high-performance liquid chromatographic method for the analysis of oxprenolol in commercial formulations is described. The analysis was performed on a cyano radial compression cartridge, with 0.0539 M, pH 3 phosphate buffer-acetonitrile-methanol (76: 15.6:8.4) as the mobile phase. The flow rate was 5 mL/min, with detection at 272 nm; the mobile phase was employed for extraction. The assay was applied to the content uniformity test of three oxprenolol hydrochloride tablet formulations of different strengths and the contents of a 2-mg dry ampule for intravenous/intramuscular injection. The percent of label claim for each formulation tested was within 91.7-110%. The applicability of this assay to the analysis of some other beta-blocking drugs was investigated. It was found that under the above conditions, atenolol, metoprolol, oxprenolol, and propranolol can be fully resolved in less than 3 min.
D000319:Adrenergic beta-Antagonists / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D010096:Oxprenolol / Q000032:analysis*; D013607:Tablets / Q000032:analysis
D000319:Adrenergic beta-Antagonists; D013607:Tablets; D010096:Oxprenolol