title: Transglutaminases and the clotting of mammalian seminal fluids.
abstract: Rapid coagulation of seminal fluid in rats, guinea pigs, and several other mammalian species including certain non-human primates is responsible for the post-coital formation of copulatory plugs in the vagina. The clotting of rodent seminal plasma results from coagulation of certain proteins derived from the seminal vesicles by enzymes secreted mainly by the coagulating (anterior prostate) gland. Several lines of evidence indicate that the clotting enzymes of coagulating gland secretions are transglutaminases, and that the extreme insolubility of the seminal clot in rodents is due to transglutaminase-catalyzed formation of epsilon(gamma-glutamyl)lysine cross-links between polypeptide chains. Various features of the apparently unique forms of transglutaminases produced by rat coagulating gland and the actions of these enzymes on vesicular secretory and other proteins are discussed. The aliphatic polyamines spermidine and spermine are incorporated covalently into the proteins of the clot as the corresponding N-mono-epsilon-(gamma-glutamyl)- and N,N-bis(gamma-glutamyl)-adducts during the enzymatic coagulation process. At the greater than millimolar concentrations at which cross-spermidine and spermine are present in normal rat seminal plasma, these polyamines attenuate the formation of hard clots in reconstituted rat semen coagulation systems, seemingly by competing with lysyl residues in vesicular secretion proteins as transglutaminase amine donor substrates, and thus preventing formation of epsilon-(gamma-glutamyl)lysine cross-bridges. It is proposed that in those species such as the rat and man in which seminal plasma contains large amounts of spermidine and(or) spermine of prostatic origin, the seminal polyamines may serve to stop blockage of the urethra by preventing too explosive a rate of seminal clot formation during the ejaculatory process.
mesh_terms: D000217:Acyltransferases / Q000502:physiology*; D000818:Animals; D003432:Cross-Linking Reagents; D005782:Gels; D006168:Guinea Pigs; D008297:Male; D011073:Polyamines / Q000494:pharmacology; D011467:Prostate / Q000201:enzymology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012661:Semen / Q000502:physiology*; D012995:Solubility; D011503:Transglutaminases
chemical_list: D003432:Cross-Linking Reagents; D005782:Gels; D011073:Polyamines; D011506:Proteins; D000217:Acyltransferases; D011503:Transglutaminases