Activity of tyrosine hydroxylase (EC 1.14.16.2) from chromaffin and nervous tissue of the Atlantic cod, Gadus morhua.
1. The activity of tyrosine hydroxylase (TH; EC 1.14.16.2) was estimated in vitro in crude tissue homogenates from the posterior cardinal veins (chromaffin tissue) and the coeliac ganglion (adrenergic neurons) of the Atlantic cod, Gadus morhua. 2. TH from the chromaffin tissue showed its highest activity at pH = 6.0 and a temperature of 30-35 degrees C, and was stimulated by low concentrations of catalase (20 micrograms/ml). 3. Estimations of the absolute TH activity in vitro showed values of 110 +/- 30 nmol/g X hr for the chromaffin tissue and 1120 +/- 280 nmol/g X hr for the coeliac ganglion.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002838:Chromaffin System / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000201:enzymology; D007700:Kinetics; D008297:Male; D009420:Nervous System / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D002374:Catalase; D014446:Tyrosine 3-Monooxygenase