[Kinetic parameters of anion adenosine triphosphatase of erythrocytes].
Effect of anions on kinetic properties of ATPase from rat erythrocyte membranes was studied. The ATPase activity was competitively inhibited by thiocyanate in mixtures containing sulfite. Thiocyanate, chlorine-anion, bicarbonate and sulfite affected noncompetitively the enzymatic activity as compared with Mg2+-ATP, ADP and calcium. Dicyclohexylcarbodiimide inhibited similarly Mg2+- and Mg2+, SO2-(3)-ATPase activities of membranes. Effect of anions on kinetic parameters of anion-dependent ATPases appears to be analogous in erythrocytes and mitochondria; the data obtained corresponded to early proposed hypothesis of the anion effect on ATPases.
D000251:Adenosine Triphosphatases / Q000097:blood*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D007700:Kinetics; D051381:Rats
D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase
Kineticheskie kharakteristiki anionnoĭ adenozintrifosfatazy éritrotisitov.