Primary amines inhibit the triggering of B lymphocytes to antibody synthesis.
Transglutaminase enzyme activity was demonstrated in murine splenic B lymphocytes. Preincubation of cell cultures with primary amines that are known substrates for transglutaminase inhibited the triggering of murine B lymphocytes to form clones of antibody-producing plasma cells. B cells could be rescued from this inhibition by incubating cultures with anti-immunoglobulin or multivalent antigens under nonstimulatory conditions. Rescue of B cells exhibited the same requirements as stimulation, which suggests that rescue is a dynamic process and not simply a receptor-ligand binding event. Primary amines appeared to inhibit an early, T cell-independent event in the B cell activation pathway, which was not sufficient to trigger B cells to form antibody-producing clones. Subsequent receptor-mediated events, required to further trigger B cells, were not inhibited by the presence of primary amines.
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001402:B-Lymphocytes / Q000276:immunology*; D004705:Endocytosis / Q000187:drug effects; D006241:Haptens; D007140:Immunoglobulin Fab Fragments; D007152:Immunologic Capping / Q000187:drug effects; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D011691:Puromycin / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D013329:Structure-Activity Relationship; D011503:Transglutaminases
D000588:Amines; D006241:Haptens; D007140:Immunoglobulin Fab Fragments; D046911:Macromolecular Substances; D011947:Receptors, Antigen, B-Cell; D011691:Puromycin; D000217:Acyltransferases; D011503:Transglutaminases