Gene expression in Acetabularia. II. Analysis of in vitro translation products.
The translation products induced by poly(A)+ RNA from Acetabularia mediterranea, A. cliftonii and A. ryukyuensis in a modified, highly efficient wheat germ cell-free in vitro system were separated by two-dimensional gel electrophoresis. A comparison of the translation products on the basis of their molecular weight and their isoelectric point revealed only a limited similarity between the patterns of the three species. The pronounced species specificity will permit the study of the in vivo translation of heterologous poly(A)+ RNA in Acetabularia cytoplasm.
D000076:Acetabularia / Q000235:genetics*; D000460:Chlorophyta / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007526:Isoelectric Point; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000096:biosynthesis; D013045:Species Specificity; D014027:Tobacco Mosaic Virus / Q000235:genetics; D014908:Triticum
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D008715:Methionine