Ammonia inhibits protein synthesis in slices from young rat brain.
Ammonium chloride, widely used as an inhibitor of lysosomal protein degradation, was shown to inhibit strongly protein synthesis in neocortical slices from 10-day-old rats at a concentration of 10 mmol/L. Its usefulness in experiments with brain tissue is doubted. The possible meaning of the finding with respect to ammonia toxicity is briefly discussed.
D000409:Alanine / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D002250:Carbon Radioisotopes; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002250:Carbon Radioisotopes; D005971:Glutamates; D009419:Nerve Tissue Proteins; D000643:Ammonium Chloride; D005973:Glutamine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D000409:Alanine