title: The participation of adenylate cyclase in lymphocyte capping.
abstract: In this study, we have observed that cells increase their intracellular cAMP to relatively high levels during receptor capping induced by either ligand-dependent (anti-Thy-1 antibody) or ligand-independent (colchicine) treatment. In addition, we have found that under capping conditions, membrane-bound adenylate cyclase is induced to co-cap with independent membrane molecules such as Thy-1 antigens. These findings suggest that the binding of anti-Thy-1 to its receptors or treatment with colchicine induces the molecular reorganization of membrane-bound adenylate cyclase which may be responsible for activating the contractile machinery required for the collection of surface receptors into a cap structure.
mesh_terms: D000262:Adenylyl Cyclases / Q000276:immunology*; D000818:Animals; D000954:Antigens, Surface; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D007152:Immunologic Capping; D066298:In Vitro Techniques; D007518:Isoantibodies; D051379:Mice; D011971:Receptors, Immunologic / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D007518:Isoantibodies; D011971:Receptors, Immunologic; D018800:Thy-1 Antigens; C035678:anti-Thy antibody; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D003078:Colchicine