title: Comparison of the effect of PGE2 and somatostatin on histamine stimulated 14C-aminopyrine uptake and cyclic AMP formation in isolated rat gastric mucosal cells.
abstract: In isolated rat gastric cells somatostatin and PGE2 were compared in respect to their effects on the cAMP system and on the histamine-stimulated H+-production, measured by 14C-aminopyrine (14C-AP) uptake. Like PGE2 somatostatin activated adenylate cyclase (AC) for all in non-parietal cells. This effect on AC declined in cell fractions with increasing number of parietal cells. Activation of AC or elevation of cellular cAMP and uptake of 14C-AP in response to histamine were inhibited by 10(-9) to 10(-5) mol/1 PGE2 and somatostatin. The results indicate remarkable similarity between somatostatin and PGE2: both activate a non-parietal cell AC and both inhibit H+-production, likely by interfering at the histamine sensitive AC of the parietal cell.
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis; D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D000242:Cyclic AMP / Q000096:biosynthesis*; D015232:Dinoprostone; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D002250:Carbon Radioisotopes; D011458:Prostaglandins E; D000632:Aminopyrine; D013004:Somatostatin; D006632:Histamine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D015232:Dinoprostone