A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double-staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin.
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003703:Demecolcine / Q000494:pharmacology; D005849:Gerbillinae; D006056:Golgi Apparatus / Q000276:immunology*; D007425:Intracellular Membranes / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D008970:Molecular Weight; D008985:Monensin; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D008985:Monensin; D013834:Thiamine Pyrophosphatase; D003703:Demecolcine