title: The adenosine deaminase binding protein of human skin fibroblasts is located on the cell surface.
abstract: We have studied the cellular localization of human adenosine deaminase binding protein in human skin fibroblasts. The binding activity sediments with the membrane fractions in a subcellular fractionation, and anti-binding protein antiserum reacts with the cell surface of intact fibroblasts, as shown by indirect immunofluorescence. The surface of intact fibroblasts binds 125I-labeled adenosine deaminase with an apparent KD of 0.26 units/ml and a maximum binding capacity of 553 milliunits/mg of cell protein. This binding is inhibited by antibinding protein antiserum. We conclude that most, if not all, of the adenosine deaminase binding protein is located on the cell surface.
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D018819:Dipeptidyl Peptidase 4; D005347:Fibroblasts / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D012867:Skin / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D002352:Carrier Proteins; D018819:Dipeptidyl Peptidase 4