Nature of the beta-adrenoreceptors in the tracheal smooth muscle of the adult pig.
Relaxant responses to isoprenaline (ISO), adrenaline (ADR) and noradrenaline (NA) were investigated on carbachol-contracted, isolated tracheal smooth muscle preparations of the adult pig to determine the nature of the beta-adrenoreceptors present. The order of relaxant potencies of the catecholamines on this preparation was ISO greater than ADR greater than NA. The potency ratio at 37 degrees C was 1:0.06:0.005. This suggests that beta 2-adrenoreceptors predominate in isolated tracheal smooth muscle of the adult pig. Though the order of potency for the above catecholamines did not change, at the lower temperatures of 29 degrees and 33 degrees C, the potency ratios were slightly modified to 1:0.0.98:0.004 and 1:0.07:0.004 respectively. pA2 values on pig tracheal smooth muscle were 8.65 and 9.13 respectively, for the non-selective beta-blockers propranolol and alprenolol, and 5.63, 5.20 and 6.26 respectively, for the beta 1 selective blockers practolol, atenolol and metoprolol and 6.82 for the beta 2 selective beta-blocker, H 35/25. The results suggest the existence and predominance of beta 2-adrenoreceptors in tracheal smooth muscle of the adult pig.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000145:classification / Q000502:physiology*; D013552:Swine; D013566:Sympathomimetics / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects / Q000294:innervation*
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D011943:Receptors, Adrenergic, beta; D013566:Sympathomimetics; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine