Induction of Epstein-Barr virus antigens and DNA-polymerase activities in P3HR-1 cell line and its subline passaged in the presence of phosphonoformate.
Lymphoblastoid cell line P3PFA was derived from P3HR-1 cells by long-term cultivation in the presence of phosphonoformate (PFA). Spontaneous production of Epstein-Barr virus antigens in PFA-selected subline was markedly reduced in comparison with the original cell line. Induction of early antigen (EA) and viral capsid antigen (VCA) syntheses with 5-iodo-2-deoxyuridine (IUDR), n-butyrate and 12-o-tetradecanoyl phorbol-13-acetate (TPA) was significantly less efficient in P3PFA than P3HR-1 cells. This was most likely associated with reduction in the number of viral genome copies per P3PFA cell that decreased more than one hundred times in comparison with P3HR-1 line. The synthesis of EA in both cell lines was not inhibited in the presence of PFA. On the other hand, PFA inhibited the synthesis of VCA by 95 and 30% in P3HR-1 and P3PFA, respectively. The relative resistance of VCA synthesis to PFA in P3PFA cells was not due to the presence of a drug-resistant virus-specific DNA-polymerase. It is noteworthy that the activity of virus-specific enzyme was markedly reduced, while cellular enzyme activity was significantly enhanced in induced P3PFA cells when compared to P3HR-1 cells treated in the same way.
D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis*; D017245:Foscarnet; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008223:Lymphoma; D019384:Nucleic Acid Synthesis Inhibitors; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D019384:Nucleic Acid Synthesis Inhibitors; D009943:Organophosphorus Compounds; D017245:Foscarnet; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid