The isolation and properties of mitochondria from rat pancreas.
A technique for the isolation of functional rat pancreatic mitochondria is described. The resultant mitochondrial preparations contained oligomycin-insensitive Mg2+-ATPase activity and coupled respiration could only be demonstrated in the absence of Mg2+ and in the presence of EDTA.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002458:Cell Fractionation / Q000379:methods; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000378:metabolism
D009840:Oligomycins; D004492:Edetic Acid; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D008274:Magnesium