Simultaneous determination of bunitrolol and its metabolite in biological fluids, plasma and urine.
A high-performance liquid chromatographic method for the simultaneous determination of bunitrolol (Koe 1366) and its metabolite, p-hydroxybunitrolol (Koe 1801) has been developed. Using the method, the sensitive and selective determination of Koe 1366 and Koe 1801 can be performed with a simple extraction with diethyl ether and spectrofluorometric detection. The detection limits of Koe 1366 and Koe 1801 in plasma are both less than 2 ng using 1-ml samples. This method was applied to human and rabbit plasma samples collected after oral administration of bunitrolol tablets (20 mg). The results show the species difference in the metabolism of bunitrolol.
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D011412:Propanolamines / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011817:Rabbits; D013045:Species Specificity
D011412:Propanolamines; C036344:4-hydroxybunitrolol