Effect of taxol on the interaction of tubulin with myosin filaments.
The interaction between polymerized tubulin from porcine brain and myosin from rabbit skeletal muscle was examined. The addition of myosin to the solution of tubulin polymerized by taxol resulted in a remarkable increase in turbidity within a few minutes at 37 degrees C, and a dense and stable precipitate was formed. The maximal molar ratio of tubulin bound to myosin was calculated to be about 4, while the value was about 2 when 6S tubulin was used. Both podophyllotoxin and colchicine suppressed the taxol-dependent increase of the binding of tubulin to myosin, but only when they were preincubated with tubulin prior to addition of taxol. 6S tubulin inhibited with actin-activated Mg2+-ATPase activity of myosin, and polymerized tubulin inhibited the Mg-ATPase more than 6S tubulin. Dense precipitates of tubulin and myosin were observed by thin-section electron microscopy. Microtubules were observed to be entangled in myosin filaments and single microtubules were occasionally surrounded by five myosin filaments in a cross section, similar to actin-myosin arrays in muscle. After incubation of tubulin with myosin, taxol was able to induce tubulin polymerization in the same way as it polymerized microtubules in the absence of myosin.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D009218:Myosins / Q000378:metabolism*; D009391:Nephelometry and Turbidimetry; D017239:Paclitaxel; D011108:Polymers / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D013552:Swine; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism*
D000470:Alkaloids; D011108:Polymers; D014404:Tubulin; D011189:Potassium Chloride; D009218:Myosins; D017239:Paclitaxel