A new cerium-based method for cytochemical localization of thiamine pyrophosphatase in the Golgi complex of rat hepatocytes. Comparison with the lead technique.
The use of cerium chloride for the localization of thiamine-pyrophosphatase (TPPase) in rat liver parenchymal cells has been investigated and the results are compared with the classical lead capture method. A medium containing 3 mM cerium chloride gave the most uniform and consistent results with a homogeneous electron dense reaction product in the first trans lamella of the Golgi complex and a weak staining of endoplasmic reticulum. The fine deposits of cerium phosphate filled completely the first trans Golgi cisterna. In contrast the reaction product of the lead-based method appeared clumpy and aggregated with an irregular distribution over both Golgi complex and endoplasmic reticulum. Higher and lower concentrations of cerium chloride than 3 mM gave inconsistent results. The present study demonstrates that the cerium-based method is superior to the classical lead-technique for the localization of TPPase.
D000818:Animals; D002563:Cerium; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D007854:Lead; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*
D007854:Lead; D002563:Cerium; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase