A new approach to quantitate the density and antigen contents of high densities of transmitter-identified terminals. Immunocytochemical studies on different types of tyrosine hydroxylase immunoreactive nerve terminals in nucleus caudatus putamen of the rat.
By means of a semi-automatic image analyzer plugged into an Apple II computer and suitable computer programs it is possible to analyze transmitter-identified nerve terminals. Thus, a densitometric approach is applied on the original photograph followed by systematic sampling which is carried out by means of a grating of circles. This procedure allows the study quantitatively of density and intensities of different types of densely packed tyrosine hydroxylase (TH) immunoreactive nerve terminals of the nuc. caudatus putamen. It is shown that the islandic TH immunoreactive nerve terminals have a higher density, but a TH content similar to the diffuse types of TH immunoreactive nerve terminals in the nuc. caudatus putamen.
D000818:Animals; D002421:Caudate Nucleus / Q000201:enzymology*; D003201:Computers; D004298:Dopamine / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D008959:Models, Neurological; D009474:Neurons / Q000201:enzymology; D011699:Putamen / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine