Simultaneous determination of triazolo-benzophenone [2',5-dichloro-2-(3-glycylaminomethyl-5-methyl-4H-1,2,4-triazol-4-yl)-benzophenone] and its major blood metabolite, triazolam, in monkey plasma by electron-capture gas--liquid chromatography.
A gas--liquid chromatographic method for the simultaneous determination of triazolo-benzophenone [2',5-dichloro-2-(3-glycylaminomethyl-5-methyl-4H-1,2,4-triazol-4-yl)-benzophenone. TB] and its major blood metabolite, triazolam, 8-chloro-6-(o-chlorophenyl)-1-methyl-4H-s-triazolo[4,3-a][1,4]fenzodiazepine (TZ), in monkey plasma was developed. Decomposition of TB was observed during gas--liquid chromatography. In alkaline medium, TB in plasma was submitted to ring closure reaction to yield triazolo-aminoquinoline, [4-amino-7-chloro-5-(2-chlorophenyl)-1-methyl-4H-s-triazolo[4,3-a]quinoline (TAQ), while TZ remained unaffected, and TAQ and TZ in the benzene extract were assayed by gas--liquid chromatography using an electron-capture detector. The concentration ranges studied were from 5 to 40 ng of TB per 0.5 ml of plasma and from 2 to 20 ng of TZ per 0.5 ml of plasma. This method could be applied to the determination of the plasma levels of TB and TZ in monkeys following intravenous administration of a single 0.2 mg/kg dose of TB.
D000818:Animals; D014151:Anti-Anxiety Agents / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D008253:Macaca mulatta; D008297:Male; D012016:Reference Values; D014149:Tranquilizing Agents / Q000097:blood* / Q000494:pharmacology; D014229:Triazolam / Q000097:blood* / Q000494:pharmacology; D014230:Triazoles / Q000097:blood* / Q000494:pharmacology
D014151:Anti-Anxiety Agents; D014149:Tranquilizing Agents; D014230:Triazoles; D014229:Triazolam; C034947:triazolo-benzophenone