[Molecular organization of glutamate-sensitive chemoexcitatory membranes of nerve cells. Binding of L-[3H]glutamate to synaptic membranes of the rat cerebral cortex].
The binding of L-[3H]glutamate to rat cerebral cortex synaptic membranes was investigated. Two types of binding sites, a Na+-independent (Kd = 140-160 nm; Bmax = 3.8-4.5 pmol-mg of protein) and a Na+-dependent (Kd = 2.0 microM; Bmax = 45-50 pmol/mg of protein) ones, were detected. The dependence of Na+-insensitive binding on time and temperature and membrane content in a sample was determined. Mono- and divalent cations (5-10 mM) potentiated specific binding by 2.1-3.3 times. The Na+-dependent binding is associated with active transport systems, while the Na+-independent one-with true receptor binding. The relationship between CNS glutamate receptors and Na+-independent binding sites is discussed.
D000818:Animals; D001693:Biological Transport, Active; D002412:Cations / Q000494:pharmacology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D017470:Receptors, Glutamate; D012964:Sodium / Q000502:physiology; D013570:Synaptic Membranes / Q000378:metabolism*
D002412:Cations; D005971:Glutamates; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D012964:Sodium
Molekuliarnaia organizatsiia glutamatchuvstvitel'nykh khemovozbudimykhmembran nervnykh kletok. Sviazyvanie L-[3H]glutamata s sinapticheskim membranami kory golovnogo mozga krys.