Fingerprint analysis of bovine hypothalamic preprosomatostatin. Identification of somatostatin-28 at the C terminus.
Messenger RNA from bovine hypothalami was used to direct the synthesis in vitro of a precursor to somatostatin (SRIF) of Mr 15,500. Specific antibodies, raised against the chemically synthesized tetradecapeptide SRIF-14, were used for the preliminary characterization. The radioactively labelled preprosomatostatin was then cleaved by trypsin or cyanogen bromide and the products were assayed by two-dimensional fingerprinting techniques. The results conclusively demonstrated the presence of the tetradecapeptide SRIF-14 sequence and its naturally occurring N-terminally extended form, SRIF-28. This 28-amino-acid sequence was shown to occupy the C terminus of the 15,500-dalton precursor and is probably preceded by basic amino acid(s).
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide; D007031:Hypothalamus / Q000378:metabolism*; D007106:Immune Sera; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000502:physiology; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D053561:Somatostatin-28; D014357:Trypsin
D007106:Immune Sera; D011498:Protein Precursors; D012333:RNA, Messenger; D013004:Somatostatin; D053561:Somatostatin-28; D014357:Trypsin; D003488:Cyanogen Bromide