The role of chloroplast and cytoplasm in the NADP-glutamate dehydrogenase and glutamine synthetase synthesis in Ankistrodesmus cells.
The effects of transcription and translation inhibitors on NADP-glutamate dehydrogenase and glutamine synthetase synthesis in nitrogen-starving Ankistrodesmus braunii cells have been studied. Considering the results obtained one can suggest that both enzymes are coded in the chloroplast genome and that during nitrogen starvation specific mRNA's are partly transferred from the chloroplast into the cytoplasm and can be translated there on 80S ribosomes.
D002701:Chloramphenicol / Q000494:pharmacology; D002736:Chloroplasts / Q000201:enzymology*; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000201:enzymology*; D056890:Eukaryota / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis*; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis*; D009249:NADP / Q000378:metabolism*; D009584:Nitrogen / Q000502:physiology; D012270:Ribosomes / Q000201:enzymology; D012293:Rifampin / Q000494:pharmacology
D009249:NADP; D002701:Chloramphenicol; D003513:Cycloheximide; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen; D012293:Rifampin