Somatostatin-like immunoreactivity in pancreatic extracts of a sahelian lizard (Varanus exanthematicus) during starvation.
In the Varanus exanthematicus, the pancreatic complex comprises the true pancreas as well as an intrasplenic islet comparable to a Brockmann's body. Somatostatin content and concentration were estimated by radioimmunoassay in acetic acid extracts of both organs. Relatively large amounts of somatostatin (SLI) are present in the pancreas (2.17 +/- 0.07 micrograms) without any difference in distribution between the cranial (CP) and mediocaudal (MCP) regions. The intrasplenic islet contains as much SLI material as the whole pancreas (3.38 +/- 0.85 microgram); thus, this primitive organ presents a very high hormonal concentration (109.34 +/- 40.30 ng/mg wt). Serial dilutions of the extracts gave parallel immunoassay displacement curves and gel-filtration revealed two immunoreactive peaks: the most important one was found in the synthetic tetradecapeptide fraction, the other one near the void volume fraction. These results show an immunological similarity between the SLI substance of the pancreatic complex and the synthetic somatostatin; however, a molecular heterogeneity must not be excluded. The results are discussed from a phylogenetic point of view.
D000818:Animals; D002850:Chromatography, Gel; D008116:Lizards / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000033:anatomy & histology / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D013004:Somatostatin / Q000378:metabolism*; D013217:Starvation / Q000378:metabolism*
D013004:Somatostatin