title: Stable isotope analysis by fast-atom bombardment labeling of UDP-glucuronic acid.
abstract: Fast-atom bombardment mass spectrometry is found to provide a method for analysis of isotopes in the enzyme cofactor uridine-5'-diphosphoglucuronic acid, heretofore unsusceptible to mass-spectral characterization. This technique was used to determine optimal conditions for the introduction of 18O by acid-catalyzed exchange in H218O and to evaluate the loss of the isotope when labeled cofactor is used in enzymatic incubations. Fast-atom bombardment mass spectrometry provided a quantitative assessment of various isotopic species and also permitted the location of the isotopes in the molecule to be determined.
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D010103:Oxygen Isotopes / Q000032:analysis*; D014535:Uridine Diphosphate Glucuronic Acid / Q000032:analysis*; D014539:Uridine Diphosphate Sugars / Q000032:analysis*
chemical_list: D010103:Oxygen Isotopes; D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid