Early changes of guanylate cyclase and cGMP phosphodiesterase activities in glomeruli and tubules isolated from the remaining kidney after unilateral nephrectomy in the rabbit.
Guanylate cyclase (GCase) and cyclic guanosine 3'5'-monophosphate-phosphodiesterase (cGMP-PDE) activities were measured in the medulla, glomeruli and cortical tubules isolated from the remaining kidney 5-120 min after unilateral nephrectomy. The results showed that cGMP-PDE-specific activities were unchanged in all the fractions isolated from the contralateral kidney when compared with the nephrectomized control kidney. The GCase-specific activity in the cortical and medullary tubules isolated from the remaining kidney reached a maximum by 10 min after unilateral nephrectomy and then returned to control values. On the other hand the glomerular GCase activity decreased for 10 min after unilateral nephrectomy, then rebound activity reached a maximum by 30 min and after 120 min had returned to the control value. The possible physiological signification of such variations is discussed.
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D006162:Guanylate Cyclase / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology; D007684:Kidney Tubules / Q000201:enzymology*; D009392:Nephrectomy; D011817:Rabbits
D015106:3',5'-Cyclic-GMP Phosphodiesterases; D006162:Guanylate Cyclase