title: Association between coated vesicles and microtubules.
abstract: In this study, a possible functional association between microtubules and coated vesicles is described. We have found that our preparations of microtubules contained coated vesicles in quantities of usually above 10%. These coated vesicles were identified both by immunological methods using anticoat antibodies and by electron microscopy of negatively stained specimens. In the immune replica, two components of coated vesicles, i.e., heavy (clathrin) and light chains, were recognized as constituents of the preparations. In the electron microscope, it was found that coated vesicles were attached predominantly along the length of microtubules. Furthermore, projections from the microtubules to the triskelion centers of the clathrin lattice were identified and thus seem to serve as linkers between the cytoskeletal structure of the organelle. A similar type of association was detected in tissue culture cells; bridges between coated vesicles and microtubules were clearly identified by electron microscopy of thin sections.
mesh_terms: D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002645:Chickens; D002966:Clathrin; D011992:Endosomes / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000032:analysis; D008854:Microscopy, Electron; D008870:Microtubules / Q000032:analysis / Q000648:ultrastructure*
chemical_list: D002966:Clathrin; D008565:Membrane Proteins