Inconsistencies detected by flow cytometry following immunofluorescence staining with anti-Thy 1 antibodies.
A number of inconsistencies have been observed when anti-Thy 1 reagents are used for immunofluorescence. (1) When indirect staining procedures are used, the differences in staining between high and low Thy 1 populations may be obscured, (2) with both direct and indirect staining methods, variable peaks of 'low Thy 1' cells can be seen, apparently derived from the high Thy 1 population by loss of antigen and/or antibody during the staining procedure. This disparity is quite variable, appears to occur at random and is independent of a large number of technical variables tested. Similar inconsistencies are not apparent with other lymphocyte markers (e.g., Lyt 1, Lyt 2, TLa, peanut agglutinin, H2, Ig).
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D018800:Thy-1 Antigens