Phospholipid degradation and edema development in cold-injured rat brain.
Development of brain edema following various pathological insults occurs after some delay. The mechanism of the delay is poorly understood. Using an in vivo model of cold-injury to study the time course of edema development, the present study indicates that the initiation of phospholipid degradation and rapid release of endogenous polyunsaturated fatty acids occurs within 1 min. Evans blue staining was slightly increased in the lesioned area at 1 min and was more profound at 30 min and at 24 h. The cerebral water content was unchanged at 1 min but was significantly increased at later times. The content of thiobarbituric acid-reactive malondialdehyde (MDA) was normal at 1 min but decreased at 30 min and at 24 h. The lipid-soluble fluorescence of MDA conjugates was also decreased concomitant with the degradation of membrane phospholipids at 24 h. Furthermore, Na+, K+-ATPase activities were consistently decreased in traumatized cortex from 24 h to 48 h after the cold-injury. These data indicate that the degradation of membrane phospholipids, the rapid release of polyunsaturated fatty acids and increased blood-brain barrier permeability are very early events underlying the subsequent development of vasogenic edema induced by cold-injury.
D015720:5'-Nucleotidase; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001812:Blood-Brain Barrier; D001834:Body Water / Q000378:metabolism; D001929:Brain Edema / Q000201:enzymology*; D001930:Brain Injuries / Q000201:enzymology*; D002540:Cerebral Cortex / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005615:Freezing; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009708:Nucleotidases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D001095:Arachidonic Acids; D004573:Electrolytes; D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides; D010743:Phospholipids; D016718:Arachidonic Acid; D005723:gamma-Glutamyltransferase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D000254:Sodium-Potassium-Exchanging ATPase