Inhibition of the adhesion of E. coli on cultured human epithelial cells in the presence of promethazine or imipramine.
The adhesion and multiplication of E. coli strain K12 with a derepressed synthesis of sex pili and of a nephropathogenic E. coli strain isolated from the urine of a patient with pyelonephritis, were demonstrated on the surface of cultured HEp-2 cells. The adhesion of the two E. coli strans was inhibited in the presence of 0.5-5.0 micrograms/ml promethazine or imipramine, but the multiplication of the bacteria on the monolayers was not decreased significantly in the presence of the two drugs. A scanning electronmicroscopic technique was developed to study bacterial adhesion and colony formation on epithelial cells.
D000268:Adhesiveness; D002294:Carcinoma, Squamous Cell; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D010861:Fimbriae, Bacterial; D006801:Humans; D007099:Imipramine / Q000494:pharmacology*; D007822:Laryngeal Neoplasms; D008855:Microscopy, Electron, Scanning; D011398:Promethazine / Q000494:pharmacology*
D011398:Promethazine; D007099:Imipramine