[Properties allowing the attribution to gamma-hydroxybutyrate the quality of neurotransmitter in the central nervous system].
gamma-Hydroxybutyrate (GHB) fulfills the main criteria of a neurotransmitter: it is unevenly distributed in C.N.S.; it is synthesized from succinic semi-aldehyde by a specific semi-aldehyde succinic reductase localized in neurons, in some dendrites and synaptic terminals; GHB is released by tissue slice depolarization, this release being reduced by 50-60% in a Ca++ free medium. Tetrodotoxin and verapamil strongly inhibited the depolarization evoked-release; high affinity heterogenously distributed binding sites for gamma-hydroxybutyrate exist in the brain. This binding does not require Na+. The bound gamma-hydroxybutyric acid is not displaceable by GABA or GABA agonists. Binding sites are enriched in the synaptosomal fraction; after micro-iontophoretic application, GHB exerts a depressant action on nigral and neocortical cells which is resistant to the presence of bicuculline methiodide. In neuronal cultures, GHB causes a hyperpolarization similar to that produced by GABA; high affinity uptake system for GHB exists both in purified plasma membrane vesicles and in brain tissue slices. This uptake is dependent on an Na+ gradient and is inhibited by ouabaïn and dinitrophenol; GABA does not modify GHB uptake by rat brain slices; GABA derived GHB has a turnover time almost three times faster than that of whole brain serotonin, 6-8 times as rapid as that of whole brain dopamine and 13-19 times as rapid as that of whole brain norepinephrine.
D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000502:physiology*; D002462:Cell Membrane; D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D006885:Hydroxybutyrates / Q000502:physiology*; D018377:Neurotransmitter Agents; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D012978:Sodium Oxybate / Q000378:metabolism / Q000502:physiology*; D013045:Species Specificity
D006885:Hydroxybutyrates; D018377:Neurotransmitter Agents; D011956:Receptors, Cell Surface; D012978:Sodium Oxybate; D006884:Hydroxybutyrate Dehydrogenase; C019416:4-hydroxybutyrate dehydrogenase
Propriétés permettant d'attribuer au gamma-hydroxybutyrate la qualité de neurotransmetteur du système nerveux central.