Characterization of progesterone-independent avidin production of chicken tissues in culture.
1. Primary cell cultures of chick oviduct, intestine, peritoneal membrane and lung, but not those of wing muscle or calcaneal tendon, produced avidin progesterone-independently through a 5-week culture. 2. Actinomycin D and cycloheximide, but not hydroxyurea, inhibited the avidin production. 3. The common feature of the cultures producing avidin was a fibroblast-like cell type. 4. Similarly, oviduct and lung, but not muscle or tendon, also produced avidin in hormone-free short-term organ cultures, where the avidin synthesis began during the first 8 h of culture. 5. Induction of the general protein synthesis does not explain the avidin production, since another egg-white protein, ovalbumin, was not induced by the culture procedure.
D000818:Animals; D001360:Avidin / Q000096:biosynthesis*; D002645:Chickens; D004054:Diethylstilbestrol / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques; D010047:Ovalbumin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D013710:Tendons / Q000187:drug effects / Q000378:metabolism*
D001360:Avidin; D011374:Progesterone; D004054:Diethylstilbestrol; D010047:Ovalbumin