[Isolation of a highly pure preparation of cephalexin amidase from bacteria of the genus Xanthomonas].
A procedure for highly purified cephalexin amidase of Xanthomonas was developed. It consists of preparation of a cell-free extract of the culture after cell disintegration, precipitation with ammonium sulfate, dissolution, concentration and elimination of ballast proteins, gel filtration on Sephadex G-25, sorption of ballast proteins on DEAE cellulose and chromatography on KM-cellulose. The enzyme yield is 45-55 per cent. The purity level is 80-90-fold.
D000581:Amidohydrolases / Q000302:isolation & purification*; D002384:Catalysis; D002458:Cell Fractionation; D002506:Cephalexin / Q000378:metabolism*; D002510:Cephalosporinase / Q000302:isolation & purification*; D006868:Hydrolysis; D008722:Methods; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000494:pharmacology; D013379:Substrate Specificity; D013381:Subtilisins / Q000494:pharmacology; D014974:Xanthomonas / Q000201:enzymology*; D001618:beta-Lactamases / Q000302:isolation & purification*
D013381:Subtilisins; D000581:Amidohydrolases; D009238:N-Acetylmuramoyl-L-alanine Amidase; D002510:Cephalosporinase; D001618:beta-Lactamases; D002506:Cephalexin
Poluchenie vysokoochishchennogo preparata tsefaleksinamidazy iz bakteriĭ roda Xanthomonas.