Preganglionic nerve stimulation increases the amount of tyrosine hydroxylase in the rat superior cervical ganglion.
Seventy-two hours after a 90 min period of electrical stimulation of the cervical sympathetic trunk, tyrosine hydroxylase activity was twice as high in homogenates of stimulated ganglia than in homogenates of contralateral control ganglia. When aliquots of the homogenates of the two groups of ganglia were mixed, the tyrosine hydroxylase activity was additive. Immunoprecipitation studies using an antibody prepared against purified tyrosine hydroxylase demonstrated that the doubling in enzyme activity is accompanied by a doubling in the amount of immunoprecipitable material. Thus the increase in tyrosine hydroxylase activity can be totally accounted for by an increase in the amount of tyrosine hydroxylase in the ganglion.
D000818:Animals; D011232:Chemical Precipitation; D004558:Electric Stimulation; D005728:Ganglia, Sympathetic / Q000201:enzymology*; D007158:Immunologic Techniques; D008297:Male; D051381:Rats / Q000502:physiology*; D011919:Rats, Inbred Strains; D013569:Synapses / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D014446:Tyrosine 3-Monooxygenase