Expression of cell surface P-glycoprotein by an adriamycin-resistant murine fibrosarcoma.
Analysis of the cell membrane of Adriamycin (doxorubicin)-resistant UV-2237 ADMR murine fibrosarcoma cells revealed a 170,000-dalton component that is not found in the drug-sensitive parent or revertant cells. Immunoblot (Western blot) analysis showed that this component is similar to the 170,000-dalton P-glycoprotein found on the surface of Chinese hamster ovary cells that exhibit multidrug resistance. Thus, multidrug resistance and P-glycoprotein expression apparently can occur in a wide variety of cells, including the metastatic murine solid tumor cell line described here.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D005354:Fibrosarcoma / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis* / Q000502:physiology; D051379:Mice
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D006023:Glycoproteins; D004317:Doxorubicin