Monoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase.
Hybridoma technology has been used successfully to generate monoclonal-antibody probes against protoplast membrane antigens. Hybridomas secreting monoclonal antibodies that either inhibit or stimulate a putative plasma-membrane marker enzyme, (K+ + Mg2+)-stimulated pH 6.5 ATPase, have been identified and cloned. The specificity of monoclonal-antibody probes on the activity of other phosphate-hydrolysing enzymes has also been examined. The production and identification of monospecific antibodies capable of immunoreacting with particular component proteins in a complex plant membrane mixture highlight the usefulness of hybridoma methodology for the enzymologist, especially since such monoclonal antibodies can be used in the purification of proteins by immunoaffinity techniques.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006824:Hybridization, Genetic; D008565:Membrane Proteins / Q000276:immunology*; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010710:Phosphates / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D011485:Protein Binding; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D010710:Phosphates; D000251:Adenosine Triphosphatases; C035214:potassium magnesium ATPase