title: Cytochemical demonstration of guanylate cyclase activity in cardiac muscle. Preferential localization at sarcolemma and junctional sarcoplasmic reticulum.
abstract: The localization of guanylate cyclase activity was cytochemically studied in heart tissue from guinea pig and pigeon. The method, based on a lead precipitation technique with GPPNHP as the substrate, was tested by quantitative biochemical analysis. The data obtained showed that in heart homogenates GPPNHP is an acceptable substrate for guanylate cyclase. The guanylate cyclase activity of glutaraldehyde prefixed heart tissue was also measured in the presence of 2 mM lead nitrate, in 30% of the untreated control hearts. The residual guanylate cyclase responded to the addition of sodium nitroprusside with a 7-fold increase in its activity. Furthermore, the guanylate cyclase requirement for Mn2+ ions was so changed by this activator that Mg2+ was as active as Mn2+. In heart muscle cells of guinea pigs and pigeons the plasma membrane of the sarcolemma and the junctional sarcoplasmic reticulum are the precipitation sites of the reaction product. In guinea pig hearts the T-tubule membranes were likewise covered with precipitates. Sodium nitroprusside stimulation of guanylate cyclase activity was indicated by increased precipitation and by shortening of the incubation time.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002712:Chlorides; D010856:Columbidae; D006162:Guanylate Cyclase / Q000032:analysis*; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D007854:Lead / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008345:Manganese / Q000494:pharmacology; D017895:Manganese Compounds; D009206:Myocardium / Q000201:enzymology*; D009566:Nitrates / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D012508:Sarcolemma / Q000201:enzymology; D012519:Sarcoplasmic Reticulum / Q000201:enzymology
chemical_list: D002712:Chlorides; D017895:Manganese Compounds; D009566:Nitrates; D015636:Magnesium Chloride; D009599:Nitroprusside; D007854:Lead; D006165:Guanylyl Imidodiphosphate; D008345:Manganese; C017461:lead nitrate; D006162:Guanylate Cyclase; D008274:Magnesium; C025340:manganese chloride