Acetyl-coenzyme A carboxylase from avocado (Persea americana) plastids and spinach (Spinacia oleracea) chloroplasts.
Preparations of acetyl-CoA carboxylase [acetyl-CoA-carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] have been obtained from the plastids of avocado (Persea americana) fruit mesocarp and from spinach (Spinacia oleracea) chloroplasts. Both preparations required bovine serum albumin, HCO3-, citrate and glycerol for stabilization. The molecular weight of the avocado enzyme was about 6.5 X 10(5) on the basis of 1 mol of biotin/mol of enzyme, the behaviour of both enzymes on gel filtration being in accord with such a value. Removal of the stabilizing bovine serum albumin resulted in the loss of a biotin-containing fragment from the avocado enzyme. Citrate stabilized the enzyme at 10 mM and activated it optimally at 3.0 mM, effecting an approx. 2-fold increase in Vmax. It is suggested that in vivo the enzyme may be located within the chloroplast lamellae.
D000103:Acetyl-CoA Carboxylase / Q000302:isolation & purification* / Q000378:metabolism; D002712:Chlorides; D002736:Chloroplasts / Q000201:enzymology*; D007700:Kinetics; D008025:Ligases / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008345:Manganese / Q000494:pharmacology; D017895:Manganese Compounds; D008970:Molecular Weight; D009940:Organoids / Q000201:enzymology*; D010944:Plants / Q000201:enzymology*; D013045:Species Specificity
D002712:Chlorides; D046911:Macromolecular Substances; D017895:Manganese Compounds; D015636:Magnesium Chloride; D008345:Manganese; D008025:Ligases; D000103:Acetyl-CoA Carboxylase; D008274:Magnesium; C025340:manganese chloride