Isolation of a cloned DNA segment containing a ribosomal protein gene of Drosophila melanogaster.
A Drosophila genomic DNA library in the vector Charon 4 was screened using cDNA derived from the small (6S-12S) poly(A)+ mRNA of 2-6-h-old Drosophila embryos. This fraction of mRNA is enriched for ribosomal protein-coding sequences. The selected recombinants were hybridized to total mRNA under conditions which allowed for isolation of homologous mRNAs. The mRNA from these RNA/DNA hybrids was eluted and translated in vitro. The translation products were analyzed by one- and two-dimensional electrophoresis with authentic ribosomal proteins as standards. One cloned DNA segment was found to contain a ribosomal protein gene, and a sequence which hybridizes strongly to at least 5 other ribosomal protein mRNAs.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005796:Genes; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D014908:Triticum / Q000378:metabolism
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D011061:Poly A; D004247:DNA