Adenylate cyclase in gastric mucosal biopsies from patients with achlorhydria. Stimulation by PGE2, histamine and gastrointestinal hormones.
Activation of adenylate cyclase (AC) by PGE2, histamine and gastrointestinal hormones was studied in parietal cell-free gastric biopsy specimens from the corpus of patients with proven high gastrin achlorhydria. PGE2, somatostatin, VIP, pentagastrin and secretin activated AC in a concentration-dependent manner both in normal and in atrophic mucosa. Histamine activated AC only in normal gastric mucosa, being entirely ineffective in mucosa devoid of parietal cells. The results indicate that histamine-sensitive AC disappears in patients with achlorhydria, probably due to their loss of parietal cells. Enzyme activity in response to somatostatin, VIP, pentagastrin, secretin and PGE2 remains unchanged in these patients indicating AC localization in nonparietal cells, e.g. chief or mucous cells.
D000126:Achlorhydria / Q000201:enzymology*; D000262:Adenylyl Cyclases / Q000032:analysis*; D000328:Adult; D000368:Aged; D015232:Dinoprostone; D004789:Enzyme Activation; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology*; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D010418:Pentagastrin / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology*; D013004:Somatostatin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D005768:Gastrointestinal Hormones; D011458:Prostaglandins E; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D006632:Histamine; D000262:Adenylyl Cyclases; D010418:Pentagastrin; D015232:Dinoprostone