Persistence of tick-borne encephalitis virus in monkeys. II. Effectiveness of methods used for virus detection.
Fifty monkeys (Macaca rhesus) inoculated with tick-borne encephalitis (TBE)virus intracerebrally or subcutaneously were examined. The efficiencies of different virus detection methods at 90 to 783 days after inoculation were: explantation of organs - 41.2%; co-cultivation of trypsinized organ cells and indicator cells in the presence of 5-bromo-2-iododeoxyuridine with detection of the virus-specific antigen by immunofluorescence - 43.7%, detection of infectious virus and complement-fixing antigen - 13.6%; immunofluorescence examination of organ impression smears - 29.3%; and examination of organ homogenates with detection of virus pathogenic for mice - 1.7%. the latter method was the least sensitive for the detection of persisting TBE virus, in spite of that in the first 3 weeks of infection its efficacy was 88.1% in examinations of clinically ill monkeys and 23.8% in the inapparent form of the infection.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D008251:Macaca / Q000382:microbiology*; D008253:Macaca mulatta / Q000382:microbiology*; D051379:Mice; D013997:Time Factors; D014775:Virus Activation; D014776:Virus Cultivation / Q000379:methods
D000956:Antigens, Viral