The low affinity component of [3H]spiperone binding reflects association to a non-dopaminergic, neuroleptic site.
The pharmacological properties of a low affinity site labelled by [3H]spiperone in membranes prepared from the rat nucleus accumbens were examined by investigating the ability of dopaminergic agonists, antagonists and various other drugs to displace the binding of 2 nM [3H]spiperone. Neuroleptic drugs were the most potent displacers of binding, and dopaminergic agonists, except for bromocryptine and lergotrile, had inhibition constants greater than 1 microM. The majority of drugs studied exhibited a high selectivity for the high affinity site labelled by [3H]spiperone and low affinity sites probably represent a non-specific, non-dopaminergic site for neuroleptic drugs.
D000818:Animals; D014150:Antipsychotic Agents / Q000378:metabolism; D001667:Binding, Competitive; D002090:Butyrophenones / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000378:metabolism*; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D012686:Septal Nuclei / Q000378:metabolism*; D013134:Spiperone / Q000378:metabolism*
D014150:Antipsychotic Agents; D002090:Butyrophenones; D011954:Receptors, Dopamine; D011955:Receptors, Drug; C028051:neuroleptic receptor; D013134:Spiperone