3-Methylcholanthrene does not induce in vitro xenobiotic metabolism in spiny lobster hepatopancreas, or affect in vivo disposition of benzo[a]pyrene.
Administration of 3-methylcholanthrene (10 mg/kg) i.m. to spiny lobsters, Panulirus argus, did not cause induction of the cytochrome P-450 content of hepatopancreas microsomes. The rate of oxidation of benzo[a]pyrene or 7-ethoxyresorufin in reductase-fortified preparations of hepatopancreas microsomes was the same for corn oil-treated or 3-methylcholanthrene-treated lobsters. Administration of 3-methylcholanthrene (10 mg/kg) i.m. to spiny lobsters one week prior to an i.v. dose of [14C]benzo[a]pyrene (1 mg/kg) did not influence the disposition of the radiolabelled benzo[a]pyrene in lobsters. At one week after the dose of [14C]benzo[a]pyrene, approximately 40% of the dose of [14C]benzo[a]pyrene remained in the lobsters, regardless of treatment. The digestive tract (hepatopancreas, intestinal contents, stomach and intestine) contained most (86%) of the 14C remaining in the lobsters.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D008861:Microsomes / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D008121:Nephropidae / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D013045:Species Specificity; D014874:Water Pollutants, Chemical
D001580:Benzopyrenes; D002273:Carcinogens; D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D009249:NADP; D008748:Methylcholanthrene; D009251:NADPH-Ferrihemoprotein Reductase