title: Effect of several biogenic amines on in vitro progesterone secretion by the bovine corpus luteum.
abstract: 1. The effect of several biogenic amines on secretion of progesterone (P4) was examined using bovine corpora lutea (n = 6), removed on day 13 of the oestrus cycle, enzymatically dispersed and cultured in vitro. 2. Luteal cell cultures were co-incubated with 0, 10, 50 or 100 ng of luteinizing hormone (LH) and 50 ng of epinephrine (EPI), norepinephrine (NOR), dopamine (DOPA), melatonin (MEL), N-acetyl-50H-serotonin (N-acetyl-50H-tryptamine; NacS), serotonin (50H-tryptamine; 5HT) or saline control. 3. EPI, NOR and DOPA decreased baseline release of P4. 4. The magnitude of the response of P4 to LH was depressed when cells were co-incubated with DOPA, EPI and 5HT and stimulated when cells were co-incubated with MEL and NacS. 5. These data indicate that the biogenic amines might modulate ovarian steroidogenesis supplementary to hypothalamic-hypophyseal hormonal mechanisms.
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000494:pharmacology*; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology
chemical_list: D001679:Biogenic Amines; D012701:Serotonin; D011374:Progesterone; D007986:Luteinizing Hormone; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine