Changes in hippocampal immunoreactive dynorphin and neoendorphin content following intra-amygdalar kainic acid-induced seizures.
Kainic acid (KA) injected focally into the amygdala induced spontaneous recurrent motor seizures. One to 6 hr after the injection of KA, the hippocampal ir-dynorphin (ir-DYN) was significantly lowered whereas 24 hr after the injection it increased. The hippocampal level of ir-alpha-neoendorphin decreased 6 hr after KA injection, and reached the control level 24 hr after the injection. Chlordiazepoxide (5 mg/kg) and phenobarbital (40 mg/kg) blocked convulsions as well as the increase in the ir-DYN content. Cycloheximide (500 micrograms icv) also antagonized the increase in the hippocampal ir-DYN. The above findings suggest that hippocampal dynorphin-related peptides are released during the seizures and that these peptides may play a physiological role in the seizure phenomena and limbic excitability.
D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D004399:Dynorphins; D004723:Endorphins / Q000378:metabolism*; D007608:Kainic Acid; D007700:Kinetics; D008297:Male; D011498:Protein Precursors / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology*
D004723:Endorphins; D011498:Protein Precursors; C025681:alpha-neoendorphin; D004399:Dynorphins; D007608:Kainic Acid