[3H] clonidine binding to rat hippocampal membranes.
Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharmacologically similar to receptors previously classified as alpha 2. A computerized model that linearized and produced the best possible fit to the experimental data points indicated that [3H] clonidine binds to a single population of receptors possessing equal affinity for the ligand. Binding data also indicated that rat hippo-campus contains significantly fewer [3H]clonidine binding sites than rat cortex.
D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D003000:Clonidine / Q000378:metabolism*; D003201:Computers; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism; D014316:Tritium
D000317:Adrenergic alpha-Antagonists; D011941:Receptors, Adrenergic; D014316:Tritium; D003000:Clonidine