Effect of D- and L-alpha-aminoadipate on the efflux of L-aspartate, L-glutamate and gamma-aminobutyrate from superfused rat brain slices.
D-alpha-Aminoadipate (D-AA) and L-alpha-aminoadipate (L-AA) were found to significantly reduce spontaneous efflux of [14C]L-aspartate from preloaded rat brain slices. Only D-AA significantly reduced spontaneous efflux of [14C]L-glutamate and [3H]gamma-aminobutyric acid (GABA); L-AA reduced but not significantly the efflux of these 2 labeled amino acids. D-AA reduced K+-stimulated release of [14C]L-aspartate and [14C]L-glutamate significantly, and L-AA that of [3H]GABA significantly. Since both D-AA and L-AA inhibit the uptake of L-aspartate, L-glutamate and GABA, their effects on the efflux of these amino acids are more specific. These results also suggest that it is unlikely that the depressant effect of D-AA, and the excitant effect of L-AA on neurons when applied locally by iontophoresis are secondary to the accelerated or decelerated release of more specific transmitter amino acids from neighboring cells.
D015074:2-Aminoadipic Acid / Q000494:pharmacology*; D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D001921:Brain / Q000187:drug effects*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D009433:Neural Inhibition / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D009435:Synaptic Transmission / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000600:Amino Acids, Dicarboxylic; D005971:Glutamates; D015074:2-Aminoadipic Acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D011188:Potassium