Monodansylcadaverine inhibition of testicular 17-ketosteroid reductase.
Dispersed mouse testicular interstitial cells were treated with the transglutaminase inhibitor monodansylcadaverine (500 microM) for 30 min. Subsequent incubation of the cells with [3H]pregnenolone increased formation of steroidogenic intermediates, tentatively identified as progesterone, 17 alpha-hydroxyprogesterone, and androstenedione, but decreased testosterone formation by monodansylcadaverine-treated cells. Measurement of 17-ketosteroid reductase activity (the enzyme that converts androstenedione to testosterone) demonstrated that monodansylcadaverine treatment caused a reversible, noncompetitive inhibition of this enzyme. These results suggest that transglutaminase catalyzed protein cross-links may influence the activity of 17-ketosteroid reductase.
D015067:17-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors*; D015068:17-Ketosteroids / Q000378:metabolism*; D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003959:Diamines; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013737:Testis / Q000201:enzymology*; D011503:Transglutaminases
D015068:17-Ketosteroids; D003959:Diamines; D015067:17-Hydroxysteroid Dehydrogenases; D000217:Acyltransferases; D011503:Transglutaminases; C008542:monodansylcadaverine; D002103:Cadaverine