A reappraisal of the stathmokinetic technique. I. An experimental study of a murine plasmacytoma with special reference to metaphase degeneration.
An "in vivo" study of metaphase degeneration in the murine plasmacytoma (MPC 11) over 8.5 h after mitotic arrest with vincristine sulphate and labelling with 3H-TdR is described. The main points to emerge were that: (1) Metaphase degeneration becomes apparent from the decline in the unlabelled metaphase collection function after 3 to 4 h; (2) the decline is such that by 8.5 h approximately 25% of the metaphases which were present in the tissue at 3 h are no longer identifiable entities; (3) No circadian variation in the mitotic index was found; (4) 3H-TdR may have a retarding effect on the entry of labelled cells into mitosis.
D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects; D003584:Cytological Techniques; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005260:Female; D007399:Interphase / Q000187:drug effects; D007700:Kinetics; D008297:Male; D008677:Metaphase / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000473:pathology*; D014750:Vincristine / Q000494:pharmacology*
D014750:Vincristine