title: Concanavalin A alters the turnover rate of tyrosine aminotransferase in cultured hepatoma cells.
abstract: Concanavalin A added to monolayer cultures of Reuber H-35 hepatoma cells caused a rapid inactivation of tyrosine aminotransferase (L-tyrosine:2-oxoglutarate aminotransferase, E.C. 2.6.1.5) and loss of reactivity with antibody against the native, dimeric enzyme. Analysis of treated cells with an antibody raised against carboxymethylated, denatured enzyme showed that the inactivated enzyme was reactive with this reagent, which does not react with the native enzyme. Subsequent addition of alpha-methyl-D-mannopyranoside to remove concanavalin A restored both enzyme activity and reactivity to antibody against native enzyme. After long-term treatment with concanavalin A, the restored enzyme levels were significantly higher than in controls treated with the sugar but not the lectin. Analysis of the turnover of the enzyme by two methods revealed that the rate of its degradation is reduced about 2-fold in concanavalin A-treated cells. Treatment with H-35 cells with concanavalin A thus effects an alteration in conformation of tyrosine aminotransferase, rendering it somewhat less sensitive to intracellular degradation.
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008766:Methylmannosides / Q000494:pharmacology; D051381:Rats; D014316:Tritium; D014444:Tyrosine Transaminase / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D008766:Methylmannosides; D014316:Tritium; D003208:Concanavalin A; C008466:methylmannoside; D014444:Tyrosine Transaminase