title: Inhibition of guanylate cyclase by guanosine 3'-phosphate and 2'-deoxyguanosine 3'-phosphate in silkworm fat body.
abstract: Guanosine 3'-phosphate and 2'-deoxyguanosine 3'-phosphate are potent inhibitors of guanylate cyclase of various tissues of the silkworm, Bombyx mori. The inhibitory activity is specific for guanine 3'-nucleotides. Thus, 2'-GMP and 5'-GMP are one order or more less potent than 3'-GMP, and the other 3'-nucleotides except 3'-IMP, i.e. 3'-AMP, 2'-deoxy-3'-AMP, 3'-CMP, and 3'-TMP, have no inhibitory activity. Guanosine and 2'-deoxyguanosine are only weakly active. The 3'-nucleotide inhibition is dependent on the concentration of Mn2+. The concentrations of 2'-deoxy-3'-GMP and 3'-GMP required for 50% inhibition are 0.12 and 0.15 mM, respectively, with 5 mM Mn2+.
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology*; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006157:Guanosine Monophosphate / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008297:Male; D009705:Nucleosides / Q000494:pharmacology; D009711:Nucleotides / Q000494:pharmacology; D011679:Pupa / Q000201:enzymology; D013329:Structure-Activity Relationship
chemical_list: D003848:Deoxyguanine Nucleotides; D006150:Guanine Nucleotides; D009705:Nucleosides; D009711:Nucleotides; C034009:2'-deoxyguanosine 3'-phosphate; D006157:Guanosine Monophosphate; D006162:Guanylate Cyclase