A cell-free soluble-phase radioimmunoassay for Thy-1 antigen.
A cell-free, soluble-phase, radioimmunoassay has been developed for Thy-1 antigen. The method is based on immunoprecipitation of radiolabelled Thy-1 molecules with specific antibodies, anti-immunoglobulin serum and polyethyleneglycol (PEG). The method can be used with convenience to screen for the presence of Thy-1 in various fluids as well as on cell surfaces for qualitative or quantitative purposes. Presence of antibodies or autoantibodies against Thy-1 can also be detected specifically. Evidence that the dog, carp, hamster and goldfish carry Thy-1-like molecules on neuronal (brain) cells is demonstrated by this method.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001921:Brain / Q000276:immunology; D002417:Cattle; D006224:Cricetinae; D004285:Dogs; D005399:Fishes; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D012016:Reference Values; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007106:Immune Sera; D018800:Thy-1 Antigens