Cell-free translation of messenger RNA for human bisphosphoglyceromutase.
mRNAs prepared from different human tissues were translated in a cell-free reticulocyte lysate system and, when present, the neosynthesized bisphosphoglyceromutase ( BPGM ) was specifically isolated by immunoprecipitation and analyzed by polyacrylamide gel electrophoresis. Analysis of the translation products showed that bisphosphoglyceromutase was synthesized in vitro with its mature molecular weight and messenger RNA specifying the synthesis of BPGM exhibited a sedimentation coefficient of 12 S in human reticulocytes. This synthesis seems to be highly tissue specific since we could not evidence any synthesis of this enzyme using mRNA obtained from non erythroid tissue. The BPGM synthesis represents 0.1% of the total neosynthesized non heme proteins in human reticulocyte and ten times less (0.01%) in human fetal liver.
D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000096:biosynthesis*; D002474:Cell-Free System; D006801:Humans; D008099:Liver / Q000032:analysis / Q000196:embryology; D010770:Phosphotransferases / Q000096:biosynthesis*; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D010770:Phosphotransferases; D001731:Bisphosphoglycerate Mutase