Inhibition of phenobarbital and dietary choline deficiency promoted preneoplastic lesions in rat liver by environmental contaminant di(2-ethylhexyl)phthalate.
The effect of di(2-ethylhexyl)phthalate (DEHP), a widely used plasticizer and environmental contaminant, on the emergence of gamma-glutamyltranspeptidase positive (GGT+) preneoplastic foci in the liver of rats fed promoting diets was studied. GGT+ foci were initiated in the liver of Sprague--Dawley male rats with a single dose of diethylnitrosamine (DEN) following partial hepatectomy. One series of control rats received saline vehicle alone. Promotion of foci was commenced by feeding: (1) a choline-deficient diet (CD); (2) a choline-supplemented diet (CS) containing 0.06% phenobarbital (CS + PHB); or (3) a CD diet containing 0.06% phenobarbital (CD + PHB). In the absence of initiation by DEN, dietary treatments did not increase the number of GGT+ foci. In rats receiving DEN, each promoting regimen effectively increased the number of GGT+ foci above levels in control rats fed only the choline-supplemented diet. Inclusion of the plasticizer at a level of 2% in each of the dietary promotion treatments, however, effectively inhibited the appearance of the foci.
D000818:Animals; D002796:Choline Deficiency / Q000150:complications / Q000473:pathology*; D004032:Diet; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000201:enzymology / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D010634:Phenobarbital / Q000037:antagonists & inhibitors*; D010795:Phthalic Acids / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000201:enzymology / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000091402:Prohibitins; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000378:metabolism
C000717332:Phb protein, rat; D010795:Phthalic Acids; D000091402:Prohibitins; D004051:Diethylhexyl Phthalate; D005723:gamma-Glutamyltransferase; D010634:Phenobarbital