Translocation of a lysosomal enzyme across the microsomal membrane requires signal recognition particle.
Signal recognition particle (SRP), an 11S ribonucleoprotein (Walter and Blobel (1982) Nature 299, 691-698), is required for translocation of secretory proteins across microsomal membranes (Walter and Blobel (1980) Proc. Natl. Acad. Sci. USA 77, 7112-7116) and for asymmetric integration into microsomal membranes of a transmembrane protein (Anderson et al., (1982) J. Cell Biol. 93, 501-506). We demonstrate here that SRP is also required for translocation of the lysosomal protease cathepsin D across microsomal membranes.
D000818:Animals; D001692:Biological Transport; D002402:Cathepsin D; D002403:Cathepsins / Q000235:genetics*; D004285:Dogs; D007425:Intracellular Membranes / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D010455:Peptides / Q000502:physiology*; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000235:genetics; D013154:Spleen / Q000201:enzymology*; D013552:Swine
D010455:Peptides; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011061:Poly A; D002403:Cathepsins; D002402:Cathepsin D