Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets.
After monolayer cultures of rat islets were exposed to [(125)I]insulin,[(125)I]glucagon, and [(125)I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells. The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells.
D000818:Animals; D002478:Cells, Cultured; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018027:Receptors, Glucagon; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism
D011956:Receptors, Cell Surface; D018027:Receptors, Glucagon; D017481:Receptors, Somatostatin; D013004:Somatostatin; D011972:Receptor, Insulin