Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes.
High resolution ('giga-seal') patch clamp recording in Xenopus oocytes was used to measure single channel currents from ACh- and GABA-activated receptors. The proteins that make up these receptors had been translated from mRNA derived from, respectively, denervated cat muscle and chick optic lobe.
D000818:Animals; D004594:Electrophysiology; D005260:Female; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics*; D011950:Receptors, Cholinergic / Q000235:genetics*; D011963:Receptors, GABA-A; D014981:Xenopus
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D011963:Receptors, GABA-A