Monitoring metabolic reactions in erythrocytes using NMR spectroscopy.
The kinetics of several metabolic reactions in intact human erythrocytes and in lysates were studied using 1H spin-echo and 13C nuclear magnetic resonance spectroscopy (NMR). The reactions monitored involved the following enzymes: (1) arginase, (2) glutathione reductase, (3) glutathione synthetase, (4) gamma-glutamyl cyclotransferase, (5) di- and tripeptidase, and (6) NAD-glycohydrolase; the first six enzymes are cytosolic whilst the latter is membrane associated. Detailed kinetics of the arginase reaction are given together with the rate of arginine transport into the cells.
D001119:Arginase / Q000097:blood; D001120:Arginine / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005978:Glutathione / Q000097:blood; D005981:Glutathione Synthase / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009699:N-Glycosyl Hydrolases / Q000097:blood; D009244:NAD+ Nucleosidase; D010084:Oxidation-Reduction; D013057:Spectrum Analysis; D005722:gamma-Glutamylcyclotransferase / Q000097:blood
D001120:Arginine; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase; D001119:Arginase; D005722:gamma-Glutamylcyclotransferase; D005981:Glutathione Synthase; D005978:Glutathione