Ca2+ ATPase inhibition in trout gill microsomes following in vitro and in vivo treatments with 2,4,5.T.
Bioassay experiments were performed after exposure for 96 hr to various concentrations of trichlorophenoxyacetic acid (2,4,5.T). The sensitivity of juvenile trout was considerably higher than that of mature animals. The activity of the microsomal Ca2+ ATPase in trout gill under the effect of 2,4,5.T was investigated. In vitro studies showed an inhibitory effect on the Ca2+ ATPase, occurring at sublethal concentrations of the herbicide. In vivo exposure to various sublethal concentrations of 2,4,5.T during 96 hr involved a significant inhibition of the microsomal Ca2+ ATPase. The sensitivity of the microsomal Ca2+ ATPase from gills to 2,4,5.T is related to the toxicity of this herbicide.
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000633:toxicity*; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D005615:Freezing; D005880:Gills / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D012487:Salmonidae / Q000378:metabolism*; D013696:Temperature; D014337:Trout / Q000378:metabolism*
D015085:2,4,5-Trichlorophenoxyacetic Acid; D000252:Calcium-Transporting ATPases