Differences in the expression of the human interferon-gamma gene in fresh lymphocytes and cultured lymphoblasts.
Fresh human peripheral blood mononuclear lymphocytes and lymphoblasts that had been grown for a period in T-cell growth-factor containing medium were stimulated with staphylococcal enterotoxin A plus mezerein to produce interferon-gamma (IFN-gamma). Growing lymphoblasts produced peak levels of IFN-gamma much earlier after induction than fresh lymphocytes. Quantitation of the steady-state levels of IFN-gamma mRNA showed these to differ markedly between the two cell types over a period of time post-induction. In fresh lymphocytes the steady-state levels of IFN-gamma mRNA increased to a peak level over a period of 4 days while in growing lymphoblasts the peak level occurred after 8 hours. These differences in IFN-gamma mRNA production were shown to be not the result of gross alteration of RNA metabolism following blast transformation.
D002478:Cells, Cultured; D004224:Diterpenes; D004768:Enterotoxins / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013729:Terpenes
D004224:Diterpenes; D004768:Enterotoxins; D007376:Interleukin-2; D010703:Phorbol Esters; D012333:RNA, Messenger; D013729:Terpenes; D011061:Poly A; C011309:mezerein; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma