Glutamine as a gamma-glutamyl donor for gamma-glutamyl transpeptidase: gamma-glutamyl peptide formation.
This study demonstrates the formation of gamma-glutamyl peptides from glutamine and plasma amino acids, as catalyzed by gamma-glutamyl transpeptidase. It also establishes the effect of various amino acids in modulating the rate of glutamine utilization as well as the hydrolytic or transfer product formed. The mechanism of the utilization of glutamine as catalyzed by gamma-glutamyl transpeptidase, involves the formation of a gamma-glutamyl enzyme bound intermediate as the initial step, with release of the amide nitrogen as ammonium, NH+4, Figure 1. The gamma-glutamyl enzyme bound intermediate either reacts with the acceptor amino acids or water; reaction with amino acids yields gamma-glutamylpeptides via the transfer pathway and reaction with water yields glutamate via the hydrolytic pathway.
D000818:Animals; D003553:Cystine / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D010452:Peptide Biosynthesis; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D005973:Glutamine; D003553:Cystine; D005723:gamma-Glutamyltransferase