title: Control of tubulin and actin gene expression in Tetrahymena pyriformis during the cell cycle.
abstract: Poly(A)-containing mRNA was isolated from division synchronized populations of the ciliated protozoan, Tetrahymena pyriformis. The level of tubulin and actin mRNA at specific cell cycle stages was analyzed by hybridization to tubulin and actin cDNA probes and by gel analysis of their in vitro translation products. The pattern of fluctuation of tubulin mRNA levels was similar to that observed for the in vivo tubulin synthesis previously reported [1]. This suggests that as the cells progress through the cell cycle, tubulin synthesis is controlled at the mRNA level. There was little fluctuation of actin synthesis or actin mRNA levels during the cell cycle, which may be indicative of a different regulatory mechanism for actin than for tubulin.
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D002453:Cell Cycle; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
chemical_list: D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin; D011061:Poly A; D004247:DNA