Inhibition of prostaglandin synthesis in rabbit kidney medulla slices by antioxidants.
The inhibitory effects of NN'-diphenyl-p-phenylenediamine (DPPD), sodium diethyldithiocarbamate (SDDC) and 2,6-di-t-butylphenol (DTBP) on the generation of medullary prostaglandin E have been compared. DPPD (1 microM) and SDDC (1 mM) failed to inhibit arachidonic acid-induced stimulation of prostaglandin E synthesis, while DTBP (1 mM) inhibited it. This suggests that DPPD and SDDC inhibit prostaglandin formation by affecting a phospholipase pathway, and the inhibition of prostaglandin generation by DTBP occurs at the cyclooxygenase step.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000494:pharmacology; D010655:Phenylenediamines / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011796:Quinacrine / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors
D000975:Antioxidants; D001095:Arachidonic Acids; D008054:Lipid Peroxides; D010636:Phenols; D010655:Phenylenediamines; D011453:Prostaglandins; D011458:Prostaglandins E; C035407:2,6-di-tert-butylphenol; D016718:Arachidonic Acid; D004050:Ditiocarb; C004845:N,N'-diphenyl-4-phenylenediamine; D011796:Quinacrine; D001241:Aspirin