The activity of membrane enzymes in homogenate fractions of rat kidney after administration of lead.
Rats received one or two consecutive daily ip injections, each 0.5 mg Pb2+/100 g body weight, and the kidneys were studied 48 or 24 hr, respectively, after the injection. Renal brush border preparations from Pb2+-treated rats exhibited significant decreases in the activity of alanine aminopeptidase and gamma-glutamyl transpeptidase, greater after two injections, yet the amount of brush border protein remained unchanged. Moreover, the activity of alkaline phosphatase in the brush border was significantly increased after Pb2+. No significant changes in urine volume, urinary protein, or enzymes could be detected in these experiments. The enzymatic changes observed in the brush border after acute exposure to Pb2+ contrasted with those after exposure to Hg2+ where both the structure and enzymatic functions were severely damaged and after exposure to Cd2+ where enzymatic alterations were not accompanied by cytological changes.
D000469:Alkaline Phosphatase / Q000378:metabolism / Q000652:urine; D000626:Aminopeptidases / Q000378:metabolism / Q000652:urine; D000818:Animals; D018826:CD13 Antigens; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D007854:Lead / Q000009:adverse effects*; D008297:Male; D008871:Microvilli / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism / Q000652:urine
D007854:Lead; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000626:Aminopeptidases; D018826:CD13 Antigens; D000254:Sodium-Potassium-Exchanging ATPase