title: Biochemical comparison of pili from variants of Neisseria gonorrhoeae P9.
abstract: Four different types of pili produced by variants of Neisseria gonorrhoeae P9 were isolated and characterized. The pili differed in subunit molecular weight with SDS-PAGE and in subunit isoelectric point on agarose gels. Isoelectric points of the major molecular species in Triton-agarose gels of octylglucoside solubilized pili were: delta, pI 6.5; alpha, pI 6.0; beta, pI 5.3 and gamma, pI 5.5. Amino acid analyses of pili showed close homology between different types but a reduction in the content of aspartate and serine was notable in the low molecular weight delta pili; also beta and gamma maps of tryptic/chymotryptic digests of pili with several major peptides apparently common to all four pilus types.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D010861:Fimbriae, Bacterial / Q000032:analysis*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000032:analysis*; D010455:Peptides / Q000032:analysis
chemical_list: D000596:Amino Acids; D010455:Peptides