The binding characteristics of some adrenergic beta-receptor antagonists to human serum proteins.
Binding of pindolol and 8 related compounds was studied in vitro by equilibrium dialysis. The overall binding in serum was compared with the binding to the main, isolated, serum proteins. Most substances show both saturable and non-saturable binding in serum. The saturable and main binding is to alpha 1-AGP, the low non-saturable binding corresponds to albumin and lipoprotein binding. The binding to alpha 1-AGP is characterized by approximately one binding site and association constants K ranging from 10(4) to 10(6) M-1. The binding of pindolol to alpha 1-AGP is strongly inhibited by propranolol, lidocaine, erythromycin, imipramine and TBEP. Significant correlations were found between log NK and log partition coefficient octanol-phosphate buffer suggesting that the protein binding of the 9 adrenergic beta-receptor antagonists to all serum proteins, including alpha 1-AGP, is predominantly hydrophobic in nature.
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009961:Orosomucoid / Q000378:metabolism; D010869:Pindolol / Q000097:blood; D011485:Protein Binding
D000319:Adrenergic beta-Antagonists; D001798:Blood Proteins; D009961:Orosomucoid; D010869:Pindolol