Effects of streptovirudin on influenza viruses type A and B: inhibition of the lipid-linked oligosaccharide synthesis of fowl plague virus.
Antibiotics of the streptovirudin complex (SV) inhibited the growth of influenza A and B viruses such as influenza A/fowl plague virus (FPV), strain Weybridge (Hav1 Neq1), influenza A/England 42/72 (H3N2), influenza A/Port Chalmers 1/73 (H3N2), influenza B/Leningrad 235/74, influenza B/Tokyo 7/66, and influenza B/Jamagata in chick embryo cell (CEC) cultures, in permanent canine kidney cells (MDCK), and in suspended fragments of chick embryo chorioallantoic membranes (CAM). As revealed by spectrophotometric turbidity measurements, SV completely inhibited the FPV-induced cytopathic effect (CPE). A 99.99% reduction of infectious virus yield was obtained in one-step growth cycle experiments and in the plaque reduction test. The haemagglutination inhibition titres of influenza viruses in suspended CAM fragment cultures in the presence of SV drugs were also substantially reduced. The incorporation assays indicated that SV exhibited no effect on virus-induced RNA synthesis, but influenced virus maturation by inhibition of lipid-linked oligosaccharide synthesis. A partial protection from infection was found in influenza virus A/England infected mice.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D009980:Influenza A virus / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009844:Oligosaccharides / Q000096:biosynthesis*; D009975:Orthomyxoviridae / Q000187:drug effects*; D011741:Pyrimidine Nucleosides; D012367:RNA, Viral / Q000096:biosynthesis; D014498:Uracil; D014779:Virus Replication / Q000187:drug effects
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D011741:Pyrimidine Nucleosides; D012367:RNA, Viral; C009329:streptovirudin; D014498:Uracil; D005947:Glucose