title: Membrane lipids and enzymes of cultured high- and low-metastatic B16 melanoma variants.
abstract: B16 melanoma cell variants were used to determine if the metastatic properties of these cells could be correlated to distinct plasma membrane, microsome, and mitochondrial membrane lipid compositions and membrane-bound enzyme activities in high- and low-metastatic cell variants, respectively. The high-metastatic B16-F10 melanoma cell membranes had lower cholesterol/phospholipid ratios, lower arachidonic acid content, lower polyunsaturated fatty acid content, higher phosphatidylcholine/phosphatidylethanolamine ratios, and higher succinate cytochrome c reductase activity than those of B16-F1 melanoma cell membranes. No differences in cholesterol/phospholipid ratio were noted in the mitochondria. Na+-K+-adenosinetriphosphatase activity and solubility of 5'-nucleotidase activity were also similar. The data indicate that the membrane lipid composition of B16-F10 melanoma cells is distinct from that of B16-F1 melanoma cells and may help to elucidate the molecular basis for the different metastatic properties of these cell lines in vivo.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D003579:Cytochrome Reductases / Q000032:analysis*; D007425:Intracellular Membranes / Q000201:enzymology* / Q000648:ultrastructure; D008545:Melanoma / Q000503:physiopathology*; D008563:Membrane Lipids / Q000032:analysis*; D051379:Mice; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D010743:Phospholipids / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D003579:Cytochrome Reductases; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase