Reduced maximum capacity of glycolysis in brown adipose tissue of genetically obese, diabetic (db/db) mice and its restoration following treatment with a thermogenic beta-adrenoceptor agonist.
The maximal activities of the key glycolytic enzymes hexokinase and 6-phosphofructokinase, were reduced in brown adipose tissue in db/db mice compared to their lean littermates. Treatment of db/db mice with the thermogenic beta-adrenoceptor agonist, BRL 26830, restored normoglycaemia. The only significant increase in activity of hexokinase and 6-phosphofructokinase in the BRL 26830-treated db/db mice occurred in brown adipose tissue where the total tissue activity increased 10- and 11-fold respectively. These changes together with increased 2-deoxyglucose uptake in vivo suggest that brown adipose tissue can play a quantitatively important role in the removal of glucose from the blood.
D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004983:Ethanolamines / Q000494:pharmacology*; D005260:Female; D006019:Glycolysis / Q000187:drug effects*; D006593:Hexokinase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009132:Muscles / Q000201:enzymology; D009765:Obesity / Q000378:metabolism*; D010732:Phosphofructokinase-1 / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; C039248:BRL 26830A; D003847:Deoxyglucose; D007655:Ketoglutarate Dehydrogenase Complex; D006593:Hexokinase; D010732:Phosphofructokinase-1