Presence of immunoreactive dynorphin in human cerebrospinal fluid.
Immunoreactive dynorphin (I-dynorphin) was measured by radioimmunoassay in human cerebrospinal fluid (CSF). I-dynorphin concentration in CSF was 30 +/- 2 pg/ml. Sephadex G-25 column chromatography showed the main peak eluted at the position of dynorphin-(1-17). HPLC elution profile of this major peak from gel filtration showed a large peak corresponding to the position of dynorphin-(1-17) and small peaks corresponding to the positions of dynorphin-(1-13), dynorphin-(1-12) and other unknown peptides.
D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000134:cerebrospinal fluid*; D006801:Humans; D008970:Molecular Weight; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D011863:Radioimmunoassay / Q000379:methods; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; C026843:dynorphin (1-13); D004399:Dynorphins; C037422:dynorphin (1-12)