title: Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain.
abstract: The uropathogenic strain Escherichia coli J96 mediates mannose-resistant hemagglutination owing to production of a digalactoside-binding adhesin. A cosmid clone from this strain has been isolated that, when harbored in E. coli K-12, expressed Pap pili and this adhesin (R. Hull et al., Infect. Immun. 33:933-938, 1981). By transposon mutagenesis and by the construction of a number of hybrid plasmid derivatives, we have demonstrated that about 8.5 kilobases of DNA is required to generate a mannose-resistant hemagglutination-positive phenotype in E. coli K-12 strain P678-54. The structural gene for the Pap pili monomer, papA, has been identified and mapped close to the promotor-proximal end of the Pap operon. Although strain P678-54 that harbored a Tn5 insertion within papA showed a mannose-resistant hemagglutination-positive phenotype, it was negative in a competitive enzyme-linked immunosorbent assay with anti-Pap pilus serum. This could mean that a Pap adhesin is encoded by a region on the Pap operon that is distinct from papA.
mesh_terms: D018830:Adhesins, Escherichia coli; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D010861:Fimbriae, Bacterial / Q000032:analysis; D005796:Genes; D005798:Genes, Bacterial; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011817:Rabbits
chemical_list: D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial