The use of aerolysin toxin as an aid for visualization of low numbers of African trypanosomes in whole blood.
A method was developed for detection of low numbers of African trypanosomes in whole blood. The method is based on selective lysis of erythrocytes and leukocytes by aerolysin, a toxin produced by the bacterium Aeromonas hydrophila. African trypanosomes do not bind the toxin and their viability and motility are therefore unaffected by this treatment.
D000333:Aeromonas; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001769:Blood / Q000469:parasitology*; D003603:Cytotoxins / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D052899:Pore Forming Cytotoxic Proteins; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000302:isolation & purification*
D001427:Bacterial Toxins; D003603:Cytotoxins; D052899:Pore Forming Cytotoxic Proteins; C032676:aerolysin