Difference between adrenergic beta 1- and beta 2-blocking effects on isoproterenol-induced Ca spike suppression in guinea-pig taenia coli.
Ca spike suppressions induced by isoproterenol (IsP) and a beta 2-agonist, 5-hydroxymethyl-6-hydroxy-2-isopropylamino-1,2,3,4-tetrahydronaphthalene -1-ol (AA497), were investigated in the presence of butoxamine or practolol. The relaxations were isotonically recorded, and the Ca spike frequency was recorded using the single sucrose gap method. IsP-induced relaxation was not inhibited by butoxamine (Butox, 0.16 microM), but was inhibited by practolol (Prac, 0.188 microM). In 24 mM K+-Krebs' solution, AA497 caused relaxation in a lower dose range and suppressed to a small extent the Ca spike frequency in a higher dose range, as was observed for IsP-induced curves of log dose-spike frequency and log dose-relaxation. In normal K+-Krebs' solution, both IsP and AA497 greatly suppressed the Ca spike frequency. IsP (1.21 microM)-induced suppression of the Ca spike frequency was blocked by Prac (113 microM), and it was blocked by Butox (96 microM) to a greater extent. AA497-induced suppression of the spikes was not blocked by Prac (37.6 microM), but completely blocked by Butox (32 microM). These selective inhibitory effects of butoxamine on AA497-induced and IsP-induced Ca spike suppression demonstrate that in the adrenergic beta-receptor-mediated process in taenia coli, beta 2-mechanisms are more closely related to the Ca spike suppression than the beta 1-mechanisms are.
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002078:Butoxamine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007422:Intestines / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009284:Naphthols / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011217:Practolol / Q000494:pharmacology
D000319:Adrenergic beta-Antagonists; D009284:Naphthols; D002078:Butoxamine; C024573:AA-497; D007545:Isoproterenol; D011188:Potassium; D011217:Practolol; D002118:Calcium