Photobiology of furocoumarins. Various types of crosslinking with DNA and their interference with the development of lambda phage.
It was shown that the multiplication of phage lambda was strongly suppressed by furocoumarins after irradiation with near ultraviolet light of 365 nm wavelength. Using xanthotoxin or angelicin there was a marked inhibition of the phage DNA injection and replication but adsorption was unaffected. This inhibition was attributed to various types of DNA crosslinking produced in the phage heads. Type I crosslink corresponded to covalent binding between adjacent sites in opposite strands of the double helix. Crosslink type II (hairpin crosslink) required a highly condensed DNA and corresponded to covalent binding between adjacent sites on double-helical segments of a folded DNA molecule. The relationships of the type I crosslinks to the DNA replication and of the type II crosslinks to DNA injection are being discussed. Like type II crosslinks, the nucleic acid--protein crosslinks hinder injection.
D000327:Adsorption; D010582:Bacteriophage lambda / Q000187:drug effects* / Q000528:radiation effects; D003432:Cross-Linking Reagents; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects; D011564:Furocoumarins / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication / Q000187:drug effects
D003432:Cross-Linking Reagents; D004279:DNA, Viral; D011564:Furocoumarins; D014764:Viral Proteins