title: Effects of inhibitors on monoamine oxidase activity in perch (Perca flavescens) brain in vitro.
abstract: 1. Perch brain homogenates were incubated in vitro and monoamine oxidase (MAO) activity was determined fluorometrically, using a kynuramine substrate. 2. Clorgyline, harmaline and deprenyl inhibited MAO activity in a concentration-related manner, with single sigmoid inhibition curves, and the type A inhibitors harmaline and clorgyline were more effective than the type B inhibitor deprenyl. 3. Two types of inhibition were recognized in vitro; a fast-onsetting inhibition, similar to that produced by a reversible inhibitor, and a slow-onsetting inhibition, which is time- and concentration-dependent and presumably represents inactivation of the enzyme.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006246:Harmaline / Q000494:pharmacology; D006912:Hydroxyquinolines / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D012642:Selegiline / Q000494:pharmacology; D013997:Time Factors
chemical_list: D006912:Hydroxyquinolines; D008996:Monoamine Oxidase Inhibitors; D012642:Selegiline; D006246:Harmaline; D003010:Clorgyline; C034010:4-hydroxyquinoline