A new pyrrolizidine alkaloid metabolite, 19-hydroxysenecionine isolated from mouse hepatic microsomes in vitro.
The in vitro mouse hepatic microsomal metabolism of the macrocyclic pyrrolizidine alkaloid senecionine was studied by high-performance liquid chromatography. A muBondapak-C18 reverse-phase system was developed to study the senecionine metabolites over a wide range of polarities. Methods were further developed for the isolation of each individual metabolite. Senecic acid, senecionine N-oxide, and 19-hydroxysenecionine, a new metabolite, were isolated from the microsomal enzyme system of BALB/c mice. The metabolite, dehydroretronecine, which had previously been isolated from rat hepatic microsomes, was not detected, and minor metabolites were not identified.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000378:metabolism*; D011763:Pyrrolizidine Alkaloids / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility
D011763:Pyrrolizidine Alkaloids; C036949:19-hydroxysenecionine