title: Independence of the androgen-induced mRNA synthesis on exogenous glucose in the ventral prostate of the rat.
abstract: Poly (A)-containing RNA species were isolated from prostatic polysomes after administration of testosterone to castrated rats. After exposure of prostatic tissue to radiolabelled uridine in vitro for 1 h the radioactivity of polysomal poly (A)-RNA increased at 6-12 h after testosterone treatment, which coincides with the hormonal activation of protein synthesis under the same experimental conditions. The increased radioactivity of polysomal mRNA was not due to changes in the uptake and phosphorylation or radiolabelled uridine or in the endogenous content of uridine nucleotides representing thus an increased mRNA content. Electrophoretic patterns of double-labelled polysomal mRNA failed to show detectable amounts of androgen-specific species. The larger part of increase in mRNA content thus consists of mRNAs common to prostatic cells of both testosterone-treated and non-treated castrated animals. The hormonal stimulation of polysomal poly (A)-RNA accumulation was not dependent upon exogenous glucose which is in contrast to protein synthesis. However, glucose was necessary for the hormonal activation of the phosphorylation of [3H]uridine leading ultimately to increased labelling of all RNA species. It is concluded that increased accumulation of polysomal mRNA may be necessary, but not sufficient for the hormonal activation of protein synthesis.
mesh_terms: D000818:Animals; D002369:Castration; D002499:Centrifugation, Density Gradient; D004586:Electrophoresis; D005947:Glucose / Q000494:pharmacology*; D008297:Male; D011061:Poly A / Q000378:metabolism; D011467:Prostate / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure; D013739:Testosterone / Q000494:pharmacology*; D014529:Uridine / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D013739:Testosterone; D005947:Glucose; D014529:Uridine