On the substrate specificities of the two forms of monoamine oxidase.
By use of the selective irreversible inhibitors clorgyline and selegiline [(-)-deprenyl], it is possible to determine the Km and Vmax values of the two forms of monoamine oxidase towards a variety of substrates. The validity of this procedure is demonstrated for the deamination of dopamine by rat liver monoamine oxidase -A and -B, and the specificities of the two forms are analysed in terms of their kinetic parameters towards different substrates.
D000818:Animals; D003010:Clorgyline / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008995:Monoamine Oxidase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D013379:Substrate Specificity
D012642:Selegiline; D008995:Monoamine Oxidase; D003010:Clorgyline; D004298:Dopamine