title: Rat lung tissue is a site of alpha 1-proteinase inhibitor synthesis: evidence by cell-free translation.
abstract: Poly(A) +RNA isolated from lungs of normal rats and of rats suffering from experimental inflammation was translated in a cell-free translation mixture from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against alpha 1-proteinase inhibitor and alpha 2-macroglobulin. Comparable levels of mRNA for alpha 1-proteinase inhibitor were found in rat lung tissue from control and experimentally inflamed animals. alpha 2-Macroglobulin mRNA could not be detected in rat lung tissue.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D011232:Chemical Precipitation; D007120:Immunochemistry; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D008297:Male; D011014:Pneumonia / Q000139:chemically induced / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014425:Turpentine; D000515:alpha 1-Antitrypsin
chemical_list: D001798:Blood Proteins; D012333:RNA, Messenger; D000515:alpha 1-Antitrypsin; D011061:Poly A; D014425:Turpentine