Reduction in eumelanin by the activation of glutathione reductase and gamma-glutamyl transpeptidase after exposure to a depigmenting chemical.
Topical application of 4-tertiary butyl catechol (TBC) causes vitiligo in the skin of man and animals, and previous electron microscopic studies showed pheomelanin formation in the affected areas. In the present study, we investigated changes of enzyme activities, eumelanin content and amount of sulfur in tissue cultured human melanoma cells exposed to the depigmenting chemical. TBC enhanced glutathione reductase activity without changing the eumelanin content by 24 hr after exposure and subsequently (by 42 hr) increased gamma-glutamyl transpeptidase activity and sulfur content in the cells with a decrease in eumelanin content. It is suggested that this chemical alters the types of melanin formed by modulation of these enzyme activities.
D002396:Catechols / Q000494:pharmacology; D002460:Cell Line; D004789:Enzyme Activation; D005980:Glutathione Reductase / Q000378:metabolism*; D006801:Humans; D008543:Melanins / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000201:enzymology; D013455:Sulfur / Q000378:metabolism; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D002396:Catechols; D008543:Melanins; D013455:Sulfur; C015047:p-tert-butyl catechol; D005980:Glutathione Reductase; D005723:gamma-Glutamyltransferase