Subcellular localization and characterization of HCO3(-)-ATPase from the mantle of the freshwater clam, Anodonta cataracta.
1. HCO3(-)-stimulated ATPase activity was demonstrated in mantle tissue of the freshwater clam, Anodonta cataracta. 2. Calcium (1 mM) slightly inhibited and SCN- completely inhibited HCO3(-)-stimulation of the enzyme. 3. ATPase activity had a Km of 6.8 mM for HCO3(-)-activation and was inhibited at HCO3(-)-concentrations greater than 20 mM. 4. Subcellular fractionation studies revealed the presence of both a mitochondrial and a non-mitochondrial HCO3(-)-ATPase.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D027321:Anion Transport Proteins; D001639:Bicarbonates / Q000494:pharmacology; D049872:Bivalvia / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D013861:Thiocyanates / Q000494:pharmacology
D027321:Anion Transport Proteins; D001639:Bicarbonates; D013861:Thiocyanates; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C031652:anion-sensitive ATPases