Inhibition of aflatoxin-initiated preneoplastic liver lesions by low dietary protein.
The development of aflatoxin B1-induced hepatic preneoplastic lesions was significantly modified by varying the level of dietary casein fed to animals during the post-dosing period. Using gamma-glutamyl transferase staining to identify areas of cellular alteration, we observed that animals fed a 5% casein diet showed a 75% reduction in lesion development as compared to animals fed a 20% casein diet. Increasing the level of dietary casein from 20% to 40% produced no further increase in response. The results of the study, showing an inhibition in the preneoplastic development by a reduction dietary casein after aflatoxin dosing, suggest that low dietary casein inhibits the promotional phase of the ultimate neoplastic response.
D016604:Aflatoxin B1; D000348:Aflatoxins; D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000009:adverse effects*; D008099:Liver / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000348:Aflatoxins; D002364:Caseins; D016604:Aflatoxin B1; D005723:gamma-Glutamyltransferase