Actions of microiontophoretically applied oxytocin, and immunohistochemical localization of oxytocin, vasopressin and neurophysin in the rat caudal medulla.
Oxytocin-, vasopressin- and neurophysin-containing axons were visualized within the rat caudal medulla using the immunoperoxidase technique. The highest densities of axons and terminals were found in the nucleus tractus solitarius, nucleus dorsalis vagus, nucleus commissuralis, nucleus reticularis lateralis and within the marginal layer of the nucleus trigeminalis. In these areas, oxytocin fibres predominated markedly over vasopressin fibres. In a series of electrophysiological experiments, neurones in these and surrounding areas were predominantly depressed following the iontophoretic application of oxytocin. This depression was seen on both spontaneous and glutamate-evoked neuronal firing.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001369:Axons / Q000032:analysis; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D007478:Iontophoresis; D008526:Medulla Oblongata / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009481:Neurophysins / Q000032:analysis*; D010121:Oxytocin / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D014018:Tissue Distribution; D014667:Vasopressins / Q000032:analysis*
D005971:Glutamates; D009481:Neurophysins; D014667:Vasopressins; D018698:Glutamic Acid; D010121:Oxytocin