Occurrence of type A and type B monoamine oxidase activities in toad egg mitochondria.
Monoamine oxidase activity has been assayed radiochemically in mitochondria from Bufo bufo eggs. Time courses of MAO activity towards PEA and 5-HT indicate that when PEA is used as a substrate higher specific activities are obtained. The inhibition patterns by clorgyline and deprenyl demonstrate that both type A and type B MAO are present. Type A activity is more sensitive than type B to the inhibitory effect of Triton X-100. Low concentrations of 2-mercaptoethanol cause a slight stimulation of type A and B activities. Increasing concentrations result in a decrease of activity.
D000818:Animals; D002023:Bufo bufo; D003010:Clorgyline / Q000494:pharmacology; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D010063:Ovum / Q000201:enzymology* / Q000648:ultrastructure; D010627:Phenethylamines / Q000378:metabolism; D012642:Selegiline / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D013379:Substrate Specificity
D007527:Isoenzymes; D010627:Phenethylamines; D012642:Selegiline; D012701:Serotonin; D008623:Mercaptoethanol; D008995:Monoamine Oxidase; D003010:Clorgyline