[Preparation of antisera against some sequences of somatostatin synthetized on resin. Application to the immunological detection of somatostatin systems: preliminary results (author's transl)].
Specific SRIF(1-14) fragments were synthetized on resin using conventional procedures. Rabbits received subcutaneously peptidyl resins in complete Freund adjuvant emulsion. The presence of antibodies was assessed by immunocytochemical and radioimmunological assays. 1. Peptidyl resins lead to antibodies production; their specificity depends on sequence and molecular configuration of the peptide on the resin. Anti-resin antibodies were not detected. 2. In the brain, SRIF(1-4) (in rat) and SRIF(10-13) (in garden-dormouse) can be demonstrated in neurophysins--positive cells of both paraventricular and supraoptic nucleus, but never in hypothalamic or extrahypothalamic SRIF(1-14)--positive neurophysin negative cells. 3. Endocrine cells of pancreatic islets contain SRIF(6-9) (in man) or SRIF(10-13) (in rat, mouse, garden-dormouse); generally, these cells are not detected by SRIF(1-14) anti-serum. Moreover, SRIF(10-13) positive cells are also detected by specific glucagon antibodies. However, it cannot be concluded that SRIF(10-13) antibodies reveal the common Thr-Phe-Thr-Ser fragment in the entire glucagon molecule. It is postulated that antibodies to several SRIF tetrapeptides reveal molecular fragments provided by the functional cleavage of an hypothetical prohormone or by the inactivation of SRIF(1-14) molecule in target cells.
D000818:Animals; D001921:Brain / Q000166:cytology; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D051379:Mice; D010179:Pancreas / Q000166:cytology; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D051381:Rats; D013004:Somatostatin / Q000032:analysis / Q000138:chemical synthesis / Q000276:immunology*
D007106:Immune Sera; D010446:Peptide Fragments; D013004:Somatostatin
Obtention d'antisérums dirigés contre certaines séquences de la somatostatine synthétisées en phase solide, et application à la détection immunocytologique des systèmes à somatostatine: résultats préliminaires.