Indirect solid-phase immunosorbent assay for detection of arenavirus antigens and antibodies.
Indirect enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (SPRIA) using either anti-human or anti-mouse IgG labelled with horseradish peroxidase and 125I, respectively, were developed for the detection of Junin, Machupo, Tacaribe, Amapari, Tamiami, Lassa and LCM arena-viruses. Both methods allow high sensitivity detection of arena-virus antigens and antibodies.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D001116:Arenaviridae / Q000276:immunology*; D006477:Arenaviruses, New World / Q000276:immunology; D001921:Brain / Q000382:microbiology; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000382:microbiology; D007836:Lassa virus / Q000276:immunology; D008099:Liver / Q000382:microbiology; D051379:Mice; D011863:Radioimmunoassay; D013154:Spleen / Q000382:microbiology
D000914:Antibodies, Viral; D000956:Antigens, Viral