Sodium butyrate stimulates somatostatin production by cultured cells.
Expression of the neuropeptide SRIF can be enhanced by sodium butyrate, a known modulator of gene transcription. Two different cell lines, RIN cells (derived from a rat pancreatic islet tumor) as well as HeLa cells, showed basal secretion of SRIF immunoreactive material that was induced between 5- and 15-fold by sodium butyrate. The induction was dose and time dependent, fully reversible, and specific for sodium butyrate. Analysis of the induced mRNAs by Northern blot hybridization using a SRIF specific cDNA probe revealed a 12-fold increase in the level of SRIF mRNA caused by butyrate treatment. These results suggest that sodium butyrate may be a useful tool for studying the regulation of SRIF gene expression.
D007516:Adenoma, Islet Cell / Q000503:physiopathology*; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002460:Cell Line; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007340:Insulinoma / Q000503:physiopathology*; D007700:Kinetics; D010190:Pancreatic Neoplasms / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013004:Somatostatin / Q000096:biosynthesis* / Q000235:genetics
D002087:Butyrates; D012333:RNA, Messenger; D020148:Butyric Acid; D013004:Somatostatin