title: The cellular mechanism of active chloride secretion in vertebrate epithelia: studies in intestine and trachea.
abstract: The cellular mechanism of active chloride secretion, as it is manifested in the intestine and trachea, appears to possess the following elements: (1)NaCl cl-transport across the basolateral membrane; (2) Cl- accumulation in the cell above electrochemical equilibrium due to the Na+ gradient; (3) a basolateral Na+-K+ pump that maintains the Na+ gradient; (4) a hormone-regulated Cl- permeability in the apical membrane; (5) passive Na/ secretion through a paracellular route, driven by the transepithelial potential difference; and (6) an increase in basolateral membrane K+ permeability occurring in conjunction with an increase in Na+-K+ pump rate. Electrophysiological studies in canine trachea support this model. Adrenalin, a potent secretory stimulus in that tissue, increases apical membrane conductance through a selective increase in Cl- permeability. Adrenalin also appears to increase basolateral membrane K+ permeability. Whether or not adrenalin also increases paracellular Na+ permeability is unclear. Some of the testable implications of the above secretion model are discussed.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D003106:Colon / Q000378:metabolism; D004285:Dogs; D004837:Epinephrine / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D007082:Ileum / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008564:Membrane Potentials; D011817:Rabbits; D012636:Secretory Rate / Q000187:drug effects; D014132:Trachea / Q000378:metabolism*
chemical_list: D002712:Chlorides; D004837:Epinephrine