title: Effect of chronic ethanol consumption on the cellular and subcellular distribution of gamma-glutamyltransferase in rat liver.
abstract: After 6 weeks of chronic ethanol consumption hepatic gamma-glutamyl-transferase and -hydrolase activities increased compared with pair-fed controls. There was no change in 5'-nucleotidase activity. It was found that the increase in gamma-glutamyltransferase activity occurred exclusively in the parenchymal cells although the principal cellular localisation for this enzyme is the biliary tract in both control and ethanol-fed rats. In both groups of animals the gamma-glutamyltransferase activities were localised by analytical subcellular fractionation techniques to soluble, plasma membrane and canalicular fractions, but the plasma membrane activity was selectively increased in the ethanol-fed rats.
mesh_terms: D015720:5'-Nucleotidase; D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D001659:Biliary Tract / Q000187:drug effects / Q000201:enzymology*; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D007728:Kupffer Cells / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D011623:gamma-Glutamyl Hydrolase; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D000431:Ethanol; D005723:gamma-Glutamyltransferase; D006867:Hydrolases; D009708:Nucleotidases; D015720:5'-Nucleotidase; D011623:gamma-Glutamyl Hydrolase