title: L-glutamic acid: a neurotransmitter candidate for cone photoreceptors in human and rat retinas.
abstract: We have combined immunocytochemical localization of L-aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1; glutamic-oxaloacetic transaminase) with autoradiographic localization of high-affinity uptake sites for L-glutamate or L-aspartate to identify the neurotransmitters of mammalian photoreceptors. In both human and rat retinas, high aspartate aminotransferase immunoreactivity is found in cones but not in rods; certain putative bipolar and amacrine cells are also heavily stained. In the human retina, and perhaps also in the rat retina, cones possess a high-affinity uptake mechanism for L-glutamate but not L-aspartate, whereas rods and Müller (glial) cells take up both L-glutamate and L-aspartate. Taken together, our results indicate that (i) L-glutamate is much more likely than L-aspartate to be the transmitter for human cones, and possibly for cones of other mammalian species as well, and (ii) major differences exist between mammalian cones and rods in the transport and metabolism or utilization of L-aspartate and L-glutamate.
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D001345:Autoradiography; D001692:Biological Transport; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D018377:Neurotransmitter Agents; D010786:Photoreceptor Cells / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014316:Tritium
chemical_list: D005971:Glutamates; D018377:Neurotransmitter Agents; D014316:Tritium; D001224:Aspartic Acid; D018698:Glutamic Acid; D001219:Aspartate Aminotransferases