[Participation of alpha-amino group of amino acids in ammonia production in heart muscle].
The ammoniogenetic capacity of some L-amino acids in rat heart muscle was studied. It was shown that [15N]leucine, [15N]glutamate and [15N]aspartate are involved in ammonia production without being the major source of this compound. The amount of [15N]ammonia produced by [15N]amino acids makes up to 0.25% of its total content. The deamination of L-[15N]leucine catalyzed by 2-oxoglutarate occurred at the highest rate. The [15N]aspartate and [15N]glutamate appeared to be less efficient precursors of ammonia. The bulk of amino nitrogen of L-[15N]amino acids was incorporated into the proteins and free amino acids in heart muscle.
D000596:Amino Acids / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009587:Nitrogen Isotopes; D051381:Rats
D000596:Amino Acids; D005971:Glutamates; D009587:Nitrogen Isotopes; D001224:Aspartic Acid; D018698:Glutamic Acid; D000641:Ammonia; D007930:Leucine
Ob uchastii al'fa-aminogrupp aminokislot v obrazovanii ammiaka v myshtse serdtsa.