title: Lipogenesis and lipolysis in normal and dwarf chickens from lines selected for high and low body weight.
abstract: Lipogenic and lipolytic comparisons were made among normal and dwarf adult males from both high-weight (HW) and low-weight (LW) selected lines. Six nonfasted birds from each of the four populations were sacrificed, and in vitro lipogenesis and lipolysis were measured in liver, adipose, and bone tissue. Also determined were activities of acetyl CoA carboxylase (E.C.6.4.1.2), NADP-malate dehydrogenase (E.C.1.1.1.40), ATP-citrate lyase (E.C.4.1.3.8), and plasma-free fatty acids (FFA). In comparison with the HW chickens, the LW males tended to exhibit higher activities of lipogenic enzymes, a greater capacity to incorporate acetate-1-14C into liver slices, an increased mobilization of FFA from adipose tissue, and high concentrations of FFA in plasma. The results indicated that the maintenance of increased postmaturational body fat associated with selection for high body weight was primarily the result of decreased lipolysis rather than enhanced lipogenesis. Effects of the dwarf allele on lipogenesis and lipolysis were not clear. Acetate incorporation into bone tissue was substantially higher than for adipose tissue, suggesting that bone may be an important site of lipogenesis in the fowl.
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D001842:Bone and Bones / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005838:Genotype; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis; D008066:Lipolysis; D008099:Liver / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D012641:Selection, Genetic
chemical_list: D005230:Fatty Acids, Nonesterified; D008055:Lipids; D008291:Malate Dehydrogenase; D001275:ATP Citrate (pro-S)-Lyase; D000103:Acetyl-CoA Carboxylase