Conformational change of Ca2+,Mg2+-adenosine triphosphatase of sarcoplasmic reticulum upon binding of Ca2+ and adenyl-5'-yl-imidodiphosphate as detected by trypsin sensitivity analysis.
Ca2+-Mg2+-ATPase of sarcoplasmic reticulum was subjected to trypic digestion under various conditions and the cleavage patterns were compared. The first tryptic cleavage to yield the NH2-terminal A-fragment (Mr approximately equal to 55,000) and COOH-terminal B-fragment (Mr approximately equal to 45,000) [Thorley-Lawson, D.A. & Green, N.M. (1977) Biochem. J. 167, 739-748] was little affected by adding ligands such as Ca2+ and AMP-P(NH)P. On the other hand, subsequent splitting of A-fragment into A1 (Mr approximately equal to 30,000) and A2 (Mr approximately equal to 20,000), and further cleavages giving rise to three smaller fragments of Mr approximately equal to 27,000-28,000 (A1a, A1b, and C) [Saito, K., et al. (1984) J. Biochem. 95, 1297] were profoundly affected by these ligands. A difference in cleavage sites was noted depending on Ca2+ ion concentration; thus, A1b and C were the major components remaining after digestion in the presence and absence of Ca2+, respectively. AMP-P(NH)P markedly stabilized both A1 and A2 fragments, but the effect was much more prominent when Ca2+ was simultaneously present on the transport site. These findings suggest that conformational changes of the ATPase molecule upon binding of Ca2+, AMP-P(NH)P, or both are accompanied by corresponding changes in the susceptibility to tryptic digestion. Fragments A1 and A2 were both quite stable and fragmentation did not proceed beyond A1, when sarcoplasmic reticulum membranes were treated with trypsin at 0 degrees C. Significant further fragmentation of A1 was observed only above 20 degrees C, suggesting a conformational transition of the ATPase protein around that temperature.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism*
D010446:Peptide Fragments; D000266:Adenylyl Imidodiphosphate; D000255:Adenosine Triphosphate; D014357:Trypsin; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium