[Submicroscopic study of adenohypophyseal cells with the hypophysis incubated in a hypertonic solution. II. Differential analysis of the intracellular distribution of arylsulfatase and thiamine pyrophosphatase within a single cell].
In the rat pituitary gland exposed to an hour incubation in the medium containing 1.65 M sucrose activities of aryl sulphatase (AS) and thiamine pyrophosphatase (TPP) were successively defined within a single specimen. After the detection and registration of both enzyme activities with the electron microscope ultrathin sections were processed with 50% H2SO4 resulting in the complete removal of the reaction products for TPP. In the mammotrophs and somatotrophs, cisternae of the Golgi apparatus and rough endoplasmic reticulum showed either AS, or TTP or both the enzymatic activities. In the latter case the two enzymes could be restricted to a single cistern.
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D005260:Female; D006982:Hypertonic Solutions; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000494:pharmacology; D013429:Sulfatases / Q000378:metabolism*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*; D013997:Time Factors
D006982:Hypertonic Solutions; D013395:Sucrose; D013429:Sulfatases; D001192:Arylsulfatases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase
Submikroskopicheskoe issledovanie adenogipofizarnykh kletok pri inkubatsii gipofiza v gipertonicheskom rastvore. II. Differentirovannyĭ analiz vnutrikletochnogo raspredeleniia arilsul'fatazy i tiaminpirofosfatazy v predelakh odnoĭ kletki.