title: Mitotic and pigment-translocating activities of cultured chromatophores of the guppy, Lebistes reticulatus.
abstract: Using Ham's F-12 medium, an in vitro culture system permitting cellular survival for over 6 months has been developed for the chromatophores of the guppy. In this culture system, the various types of chromatophores (melanophores, erythrophores and xanthophores) migrated out of the explanted tail fin tissue, retained their pigmentation, and displayed both mitotic and pigment-translocating activities. The mitotic activity was evident during the first 3 or 4 weeks in culture, whereas the pigment-translocating ability persisted for 16 weeks. The cultured chromatophores of male fish displayed pigment aggregation in response to adrenergic agents (epinephrine and norepinephrine) and pigment dispersion in response to alpha-melanocyte stimulating hormone (alpha-MSH), cyclic AMP and dibutyryl cyclic AMP. Cyclic GMP did not elicit pigment-translocating responses in any of the chromatophores.
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000378:metabolism; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D002856:Chromatophores / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D010860:Pigments, Biological / Q000378:metabolism*; D012880:Skin Pigmentation / Q000187:drug effects
chemical_list: D010860:Pigments, Biological; D003994:Bucladesine; D009074:Melanocyte-Stimulating Hormones; D000242:Cyclic AMP