Bombesin and calcitonin secretion by pulmonary carcinoma is modulated by cholinergic receptors.
Three established cell lines derived from human small cell carcinoma of the lung, and known to produce significant amounts of peptide hormones were used to evaluate the regulation of hormone secretion by cholinergic agonists. In two of the cell lines (DMS 53, DMS 153) acetylcholine chloride, bethanechol chloride, and carbamylcholine at the concentrations of 10(-3)M to 10(-5)M stimulated secretion of bombesin and calcitonin as measured by RIA. The third cell line, DMS 406, was not significantly stimulated. Inhibition of induced stimulation by the cholinergic antagonist atropine, but not hexamethonium, indicated the presence of muscarinic rather than the nicotinic type of cholinergic receptors on the stimulatable cells. These receptors appear to mediate hormone secretion comparably to normal endocrine cells.
D000109:Acetylcholine / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology; D001839:Bombesin / Q000378:metabolism*; D002116:Calcitonin / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002460:Cell Line; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology*
D001626:Bethanechol Compounds; D006584:Hexamethonium Compounds; D010455:Peptides; D011950:Receptors, Cholinergic; D018723:Bethanechol; D018738:Hexamethonium; D001285:Atropine; D002217:Carbachol; D002116:Calcitonin; D000109:Acetylcholine; D001839:Bombesin