title: Ammonia inhibits protein synthesis in slices from young rat brain.
abstract: Ammonium chloride, widely used as an inhibitor of lysosomal protein degradation, was shown to inhibit strongly protein synthesis in neocortical slices from 10-day-old rats at a concentration of 10 mmol/L. Its usefulness in experiments with brain tissue is doubted. The possible meaning of the finding with respect to ammonia toxicity is briefly discussed.
mesh_terms: D000409:Alanine / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D002250:Carbon Radioisotopes; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D005971:Glutamates; D009419:Nerve Tissue Proteins; D000643:Ammonium Chloride; D005973:Glutamine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D000409:Alanine