The development of mixed function amine oxidase in cultured foetal rat hepatocytes and its relation to 3'-methyl-4-N,N-dimethylaminoazobenzene effects on tyrosine aminotransferase accumulation.
The ability of cultured foetal rat hepatocytes to metabolize the carcinogen 3'-methyl-4-dimethylaminoazobenzene (MDAB) is shown to correlate with the effectiveness of the carcinogen in suppressing the accumulation of tyrosine aminotransferase (TAT). MDAB is ineffective in cultures of 15-day gestation liver which are unable to carry out oxidation of MDAB as judged by the conversion of [3H]MDAB to a non-ether extractable form. In contrast, 19-day gestation hepatocytes can perform this function, and correspondingly the levels of TAT are suppressed in these cultures in the presence of MDAB. When 15-day gestation hepatocytes are maintained for beyond 3 days in culture, they acquire the ability to oxidize MDAB and accordingly become susceptible to the carcinogen.
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000502:physiology; D005865:Gestational Age; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D008749:Methyldimethylaminoazobenzene / Q000378:metabolism / Q000633:toxicity*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D014444:Tyrosine Transaminase / Q000378:metabolism*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D008996:Monoamine Oxidase Inhibitors; D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene; D006899:Mixed Function Oxygenases; D014444:Tyrosine Transaminase