Ultrastructural studies on the replication of herpes simplex virus in PK and XTC-2 cells.
Ultrastructural changes showed the following characteristics of restricted replication of herpes simplex virus 1 (HSV 1) strains MA and HSZP in PK and XTC-2 cells: 1) minimal cytopathic changes in PK cells as compared to more pronounced alterations in XTC-2 cells; 2) formation of single nucleocapsids or their absence in the nuclei of PK cells infected with the HSZP strain; 3) lack of budding and envelopment and absence of reduplication of the nuclear membrane; 4) persistence of partially uncoated virions within the endocytic vacuoles in the cytoplasm of PK cells; and 5) formation of dense inclusion bodies in addition to the presence of defective virions in the cytoplasm of XTC-2 cells and vacuolation of their cytoplasmic membranes. The replication of HSV 1 in PK and XTC-2 cells seemed to be blocked at both early and late stages of virus replication. At low multiplicity of infection, no virus particles were formed.
D000818:Animals; D002460:Cell Line; D007668:Kidney; D008168:Lung; D008854:Microscopy, Electron; D011817:Rabbits; D018139:Simplexvirus / Q000502:physiology / Q000648:ultrastructure*; D013552:Swine; D014779:Virus Replication; D014982:Xenopus laevis