title: Expression of differentiation markers by chick embryo neuroretinal cells in vivo and in culture.
abstract: Several markers of chick neuroretinal differentiation were monitored in vivo and in culture. All increase markedly between 7 and 20 days of embryonic development in vivo. In vitro, endogenous GABA levels decrease almost immediately, while other neuronal markers increase as in vivo for 2 to 5 days before declining (choline acetyltransferase, acetyl cholinesterase, glutamic acid decarboxylase). Neuronal cell surface markers (binding sites for tetanus toxin, alpha-bungarotoxin, muscimol), however, reach maximal levels only after 8 days in vitro. Glial markers such as carbonic anhydrase and hydrocortisone-induced glutamine synthetase activities are also expressed only transiently in culture.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism; D011963:Receptors, GABA-A; D011978:Receptors, Nicotinic; D012160:Retina / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D064569:alpha7 Nicotinic Acetylcholine Receptor; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D011963:Receptors, GABA-A; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; D005680:gamma-Aminobutyric Acid; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D005968:Glutamate Decarboxylase; D002256:Carbonic Anhydrases; D005974:Glutamate-Ammonia Ligase