Enzymatic inhibition of lysine transport across rat and turtle small intestine.
Trypsin, Phospholipase-D and Neuraminidase at different concentrations inhibited significantly (P less than 0.001) lysine accumulation in rat intestinal cells, whereas Chymotrypsin did not show any significant effect. Trypsin, Chymotrypsin and Phospholipase-D did not show any significant change in lysine accumulation when incubated with mucosal strips isolated from turtle intestine; however, Neuraminidase caused a significant decrease in lysine uptake by these strips. Unidirectional lysine influx across the rat small intestine was significantly reduced (P less than 0.01) by the presence of Trypsin, Phospholipase-D and Neuraminidase in the preincubation solution, and its influx across the turtle small intestine was significantly inhibited (P less than 0.01) by Neuraminidase only. The cell water content and the cell volume were not altered by preincubating the intestinal tissues with any of the enzymes in both animals.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002918:Chymotrypsin / Q000494:pharmacology; D006867:Hydrolases / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D010739:Phospholipase D / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014357:Trypsin / Q000494:pharmacology; D014426:Turtles
D006867:Hydrolases; D010739:Phospholipase D; D009439:Neuraminidase; D002918:Chymotrypsin; D014357:Trypsin; D008239:Lysine