Effect of Chinoin-103, a new antiarrhythmic drug, on the transmembrane potentials, ionic currents and contractile force in heart muscle.
The effect of Chinoin-103 (CH-103) was examined on the electrophysiological and mechanical properties of the isolated guinea-pig atrial and ventricular myocardium and on membrane ionic currents of the frog atrial trabeculae using glass-microelectrode and voltage clamp techniques. In the guinea-pig atrial and ventricular myocardium, CH-103 had no effect on the resting membrane potential, but decreased the action potential overshoot and maximum rate of depolarization (Vmax). CH-103 exerted a negative inotropic effect on both preparations. The drug (10(-5) M) consistently shifted the membrane responsiveness curve to more negative potentials. CH-103 rapidly abolished the membrane electrogenesis in myocardial preparations pretreated with 5 mM MnCl2. The slow action potentials induced in K+-depolarized preparations by caffeine were slightly reduced, whereas the isoproterenol-induced ones were markedly depressed by the drug. In voltage clamp experiments, CH-103 strongly reduced the fast inward Na+ current, but hardly decreased the slow inward Ca2+ current and the delayed outward K+ current. These results suggest that CH-103 has both membrane stabilizing and beta-blocking effects, and can explain the antiarrhythmic action of CH-103.
D000200:Action Potentials / Q000187:drug effects; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000605:Amino Alcohols / Q000494:pharmacology*; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D016275:Atrial Function; D002118:Calcium / Q000378:metabolism; D003864:Depression, Chemical; D004558:Electric Stimulation; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D010210:Papillary Muscles / Q000502:physiology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; D000605:Amino Alcohols; D000889:Anti-Arrhythmia Agents; C014893:naphthoxybutanolcyclohexylamine; D012964:Sodium; D011188:Potassium; D002118:Calcium