Localization of a narrow-specificity thyroliberin hydrolyzing pyroglutamate aminopeptidase in synaptosomal membranes of guinea-pig brain.
In this paper we report the presence of a particulate pyroglutamate aminopeptidase in guinea-pig brain tissue. This activity appears to reside in the synaptosomal membrane and could be released from the membrane by treatment with papain or Triton X-100. By contrast with a previously described broad-specificity soluble pyroglutamate aminopeptidase from guinea-pig brain tissue, the enzyme released from the synaptosomal membrane preparation removed pyroglutamic acid from thyroliberin, acid thyroliberin and less than Glu-His-Gly alone of the peptides tested. Unlike the soluble tissue enzyme the present enzyme was inhibited by the presence of EDTA and the activity released from synaptosomal membranes by papain was found to have a relative molecular mass of 230 000, almost one order of magnitude greater than that reported for the soluble enzyme.
D000626:Aminopeptidases / Q000302:isolation & purification*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002850:Chromatography, Gel; D004586:Electrophoresis / Q000379:methods; D006168:Guinea Pigs; D006868:Hydrolysis; D017830:Octoxynol; D010206:Papain; D011092:Polyethylene Glycols; D011751:Pyroglutamyl-Peptidase I / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity; D013570:Synaptic Membranes / Q000201:enzymology; D013574:Synaptosomes / Q000201:enzymology*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
D011092:Polyethylene Glycols; D013973:Thyrotropin-Releasing Hormone; D017830:Octoxynol; D000626:Aminopeptidases; D011751:Pyroglutamyl-Peptidase I; D010206:Papain