The sulphatase of ox liver. XXIV. The glycosulphatase activity of sulphatase a.
The rhodizonic acid method for the determination of SO2-4 has been used to investigate the glycosulphatase activity of the sulphatase A (aryl-sulphate sulphohydrolase, EC 3.1.6.1) of ox liver. Sulphatase A hydrolyses D-glucopyranose and D-galactopyranose 2-, 3-, 4- and 6-sulphates: glucose sulphates are hydrolysed more rapidly than galactose sulphates and the 3-sulphates more rapidly than the other isomers. 2-Acetamido-2-deoxyglucopyranose 6-sulphate is not hydrolysed, nor is 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranose 1-sulphate. Sulphate is a competitive inhibitor of the glycosulphatase activity. Hydrolysis proceeds through fission of the O-S bond. Evidence is given that the hydrolysis of glucose 3-sulphate is accompanied by the formation of substrate-modified sulphatase A, although this has not been isolated. Sulphatase A has no detectable alkylsulphatase activity.
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D002417:Cattle; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013429:Sulfatases / Q000378:metabolism*
D013429:Sulfatases; D001192:Arylsulfatases; D002553:Cerebroside-Sulfatase