Effect of 13-cis retinoic acid on fatty acid composition and membrane-associated enzymes in rat submandibular salivary glands.
Weanling rats were fed for 4 weeks semipurified diets containing 0, 100 or 300 mg 13-cis retinoic acid per kg diet. The activities of (Na+ + K+)-ATPase, Mg2+-ATPase and gamma-glutamyltranspeptide were measured in submandibular salivary gland (SMSG) homogenates. The activity of (Na+ + K+)-ATPase in the SMSG was reduced in rats fed 13-cis retinoic acid. There was no effect on the activities of the other two enzymes. Fatty acid composition of total lipids in SMSG suggest that decrease in (Na+ + K+)-ATPase activity may be associated with changes in fatty acid composition of total lipids.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase; D002462:Cell Membrane / Q000201:enzymology; D005227:Fatty Acids / Q000378:metabolism*; D015474:Isotretinoin; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005227:Fatty Acids; D014212:Tretinoin; D005723:gamma-Glutamyltransferase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D015474:Isotretinoin