Further evidence for lack of specific receptors for PGE2 in the rat ovary.
Prostaglandin E2 (PGE2) seems to stimulate cAMP accumulation in ovaries of all mammals. While it acts through specific receptors in some species, our earlier observations (1) suggest absence of PGE2 receptors in the rat ovary. In order to further substantiate this assumption we digested ovarian membranes from the bovine and the rat with various enzymes and measured cAMP after stimulation with PGE2, NaF, and hCG. Pronase, trypsin, and phospholipase C abolished cAMP accumulation completely. Neuraminidase, beta-galactosidase and phospholipase D did not interfere with cAMP formation. After treatment with phospholipase A2, PGE2-mediated cAMP accumulation was abolished in the bovine but not in the rat ovary. Formation of cAMP disappeared after hCG but not after NaF in both species. Furthermore specific binding of PGE2 could not be demonstrated in phospholipase A2-treated bovine ovaries. These findings are consistent with presence of specific PGE2 receptors in the bovine and their absence in the rat ovary.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D015232:Dinoprostone; D004789:Enzyme Activation; D005260:Female; D007700:Kinetics; D009439:Neuraminidase / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*; D011982:Receptors, Prostaglandin / Q000032:analysis*; D018078:Receptors, Prostaglandin E; D012969:Sodium Fluoride / Q000494:pharmacology; D013045:Species Specificity; D001616:beta-Galactosidase / Q000378:metabolism
D006063:Chorionic Gonadotropin; D011458:Prostaglandins E; D011956:Receptors, Cell Surface; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D012969:Sodium Fluoride; D010740:Phospholipases; D009439:Neuraminidase; D001616:beta-Galactosidase; D010447:Peptide Hydrolases; D000262:Adenylyl Cyclases; D015232:Dinoprostone