title: The current-voltage behavior of ion channels: important features of the energy profile of the gramicidin channel deduced from the conductance-voltage characteristic in the limit of low ion concentration.
abstract: The conductance-voltage (G-V) characteristic of a single-filing, multi-barrier, multi-occupancy channel depends in the limit of low ion concentration upon only two parameters: the voltage dependence of the entry step and the ratio of the rate constant for leaving the channel to that for crossing its middle (14,17,20). We show that the G-V shape in this low concentration limit  can be measured accurately using a triangular wave, many-channel technique and demonstrate that the observed shape is incompatible with that expected if the only important rate limiting barrier at low concentration were at the channel mouth. Instead the central barrier turns out, surprisingly, in view of the markedly sublinear I-V shape at low concentration, to be even slightly larger than the exit barrier. Additionally, we find that it is not possible to fit both the G-V shape and the concentration dependence of the zero-current conductance simultaneously with a 3-barrier 2-site model. However, by adding additional sites to yield a 3-barrier 4-site model either of the type 3B4S" where the extra site in each channel half is external to the mouth of the channel or of the type 3B4S' where the extra site is internal to the mouth of the channel, we obtain good agreement. Additionally, using the flux ratio data of Procopio and Andersen (19) to discriminate between 3B4S and 3B4S" models, we find the 3B4S" model to be the only satisfactory one.
mesh_terms: D004573:Electrolytes / Q000378:metabolism; D006096:Gramicidin / Q000378:metabolism*; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers / Q000378:metabolism; D008564:Membrane Potentials
chemical_list: D004573:Electrolytes; D007473:Ion Channels; D008051:Lipid Bilayers; D006096:Gramicidin