Inhibition of vaccinia mRNA methylation by 2',5'-linked oligo(adenylic acid) triphosphate.
Extracts of interferon-treated cells synthesize unique 2',5'-linked oligo(adenylic acid) 5'-phosphates in the presence of ATP and double-stranded RNA. 2',5'-linked oligo(adenylic acid) 5'-triphosphate inhibits protein synthesis at nanomolar concentrations by activating RNase. We have observed that oligo(adenylic acid) 5'-monophosphate and 5'-triphosphate are potent inhibitors of vaccinia mRNA methylation in vitro. Both the methylation of mRNA methylation is not due to degradation of the mRNA. Inhibition of the 5'-terminal guanine at the 7 position and the 2'-O-ribose methylation of the penultimate nucleoside are inhibited. Such inhibition of the requisite modification of the 5' terminus of mRNA by 2',5'-linked oligo(adenylic acids) may be a mechanism of interferon action against both DNA and RNA viruses in which mRNAs derived from them are capped.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells; D008745:Methylation; D008780:Methyltransferases / Q000037:antagonists & inhibitors; D051379:Mice; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D014616:Vaccinia virus / Q000235:genetics*; D014779:Virus Replication
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; C023505:2',5'-oligoadenylate; D007372:Interferons; D008780:Methyltransferases