Chlorophyll fluorescence as a probe for the determination of the photo-induced proton gradient in isolated chloroplasts.
Gramicidin D-treated chloroplasts show an acid-induced quenching of the chlorophyll fluorescence, which is composed of a reversible and irreversible part. The reversible quenching is analogous to the photo-induced quenching in coupled chloroplasts and can be taken to determine the light induced delta pH.
D002734:Chlorophyll / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D006096:Gramicidin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D010944:Plants / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D006096:Gramicidin; D002734:Chlorophyll