title: Idiotype-specific tolerance: preferential alteration of idiotype expression after neonatal exposure to tolerogen.
abstract: The development of HOPC-8 idiotype-positive and HOPC-8 idiotype-negative B cell subpopulations involved in the immune response to a single PC antigen, has been monitored during normal differentiation and in situations where neonatal tolerance had been induced. It was found that H8 id+ and H8 id- anti-PC antibody-secreting cells appeared at approximately the same time in ontogeny and these late maturing subsets constituted equal fractions of the immune response in CBA/CaJ X BALB/c F1 (JBF1) mice. However, when JBF1 mice were injected at birth with graded doses of PC-FGG, responses obtained in 4-week-old mice were predominantly of the H8 id. This PC-specific, idiotype-restricted, unresponsive state was stable upon adoptive transfer. Specific depletion of Thy-1.2-positive cells and in vitro "mixing" experiments involving combined ratios of tolerant and nontolerant spleen cells suggest that the unresponsive state measured 4 weeks after tolerogen administration does not involve suppressor cells. It would appear that intrinsic differences between these idiotypically defined B cell subsets account for the observed differential susceptibility to tolerance induction among PC-responsive B cells.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000941:Antigens; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology; D002645:Chickens; D000939:Epitopes; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010767:Phosphorylcholine / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology
chemical_list: D000941:Antigens; D000942:Antigens, Bacterial; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine