Lectin staining of carbohydrates of haemic cells. II. The cells of normal lymphoid origin, of lymphatic leukaemias and related diseases.
Normal and leukaemic lymphoid cells, both human and murine, were stained for specific carbohydrates with three fluorescein-labelled lectins: Aprotinin for sialyl (or uronyl) groups: Ricinus agglutinin for galactosyl groups; and Concanavalin A for mannosyl (or glucosyl) groups. The method gives permanent preparations of sections from methanol fixed, paraffin embedded tissues, from blood and bone marrow films or touch preparations of lymph nodes that were methanol fixed. Whereas normal lymphocytes and lymphoblasts reacted strongly for sialyl groups, lymphoblasts of acute lymphoblastic leukaemia and lymphocytes of chronic lymphocytic leukaemia gave a much weaker reaction. The same was the case of the lymphocytes of the Sézary variant and the lymphocytes of macroglobulinaemia. The fine processes of the cells of hairy cell leukaemia stained well for sialyl groups. No obvious differences were detected between normal monocytes and the cells of monocytic leukaemia, nor between normal plasma cells and those of myeloma.
D000818:Animals; D007611:Aprotinin; D002241:Carbohydrates / Q000032:analysis*; D003208:Concanavalin A; D005452:Fluoresceins; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins; D007938:Leukemia / Q000473:pathology*; D007943:Leukemia, Hairy Cell / Q000473:pathology; D007945:Leukemia, Lymphoid / Q000473:pathology; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D008221:Lymphoid Tissue / Q000032:analysis*; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology; D008258:Waldenstrom Macroglobulinemia / Q000473:pathology
D002241:Carbohydrates; D005452:Fluoresceins; D037102:Lectins; D003208:Concanavalin A; D007611:Aprotinin