Correlation of hnRNP structure and nascent transcript cleavage.
Using electron microscopy of spread chromatin, we have observed nonnucleolar transcription units from Drosophila melanogaster and Calliphora erythrocephala that display specific cleavage of nascent transcripts. We have quantitatively analyzed 20 of these relatively long transcription units. The primary RNP structure of homologous transcripts is nonrandom with respect to both RNA sequence and the cleavage event. In general, released RNA fragments have a smooth fibrillar RNP morphology (approximately 50 A wide) and retained segments have a thicker particulate morphology (approximately 250 A diameter). A characteristic secondary structure formation also accompanies cleavage--that is, RNP fibril loops form by association of noncontiguous transcript sequences that correspond to the terminal regions of the segment to be released. RNP particles form at the loop base sequences prior to their association and apparently coalesce upon loop formation. These loops, and thus the released segments, range in length from 1 and 25 kb on the examples we have analyzed. Cleavage of nascent hnRNA transcripts appears to be a fairly common event in these organisms and occurs within 0.3-3 min after transcription of the cleavage site.
D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure; D004175:Diptera / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000032:analysis; D011487:Protein Conformation; D012313:RNA / Q000032:analysis*; D012261:Ribonucleoproteins / Q000032:analysis; D014158:Transcription, Genetic
D002843:Chromatin; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D012313:RNA