[Absorption of the antibody formation to anticartilage-antiparathyroid antibodies administered into the rectum (author's transl)].
The absorption of and specific antibody formation to Artroglobina (:AG, beta and gamma-globulin fractions of anticartilage-antiparathyroid immune serum from the horse) administered into the rectum were studied in rats and guinea-pigs, an immunological tests in the "Phase I Study" were carried out using serum from volunteers. 1) When the radio-labelled AG (125I-AG) was administered into the rat rectum, the plasma level of 125I reached the maximum (3.1% of the total dosage) at 2 hour later and 0.3 per cent of the total dosage retained the reactivity with the anti-Fab antibody. 2) Gel diffusion and PCA tests gave positive reactions in the sera after the second administration of AG in a dose of 20 mg/kg, corresponding to 40 times the clinical dose, with a certain interval after the first treatment. From the results, it was confirmed that rectally-administered AG induces mainly IgG or IgE antibody formation, in the rat. 3) The administration of AG through the rectum did not induce the systemic anaphylaxic reaction in the guinea-pigs which had been administered AG into the rectum. The intravenous challenge, however, resulted in a positive anaphylaxic reaction in animals treated with AG rectally. This indicates the sensitization of the animal with AG by the rectal administration. 4) The specific antibody responses, immune complexes and complement values did not change in the sera of volunteers given AG which consisted of alternating cycles of twelve days of treatment with one suppository daily, and twelve days without treatment, in a clinical dose of 25 mg. Nevertheless, AG must be carefully prescribed as higher doses given to experimental animals induced adverse immunological effects.
D000707:Anaphylaxis; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000918:Antibody Specificity; D002356:Cartilage / Q000276:immunology*; D003165:Complement System Proteins / Q000032:analysis; D004341:Drug Evaluation; D006168:Guinea Pigs; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D010280:Parathyroid Glands / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012007:Rectum / Q000378:metabolism; D005719:gamma-Globulins / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; C033161:artroglobina; D005719:gamma-Globulins; D003165:Complement System Proteins