Influence of D-galactosamine and alpha-naphthylisothiocyanate on the activities of some intestinal enzymes.
The activities of lactase, sucrase, maltase and gamma-glutamyl transferase (gamma-GT) were determined in homogenates of rat jejunal mucosa 24 h after acute administrations of D-galactosamine (GALN) (1.855 mmol/kg; i.p. injection) and alpha-naphthyl-isocyanate (ANIT) (0.540 mmol/kg; given by gastric tube). The animals were fasted either 24 h or 72 h prior to sacrifice. In rats fasted only 24 h, GALN treatment resulted in a pronounced decrease in lactase and in a moderate elevation of sucrase and maltase. ANIT clearly reduced lactase and, to a lesser extent, sucrase, while it increased maltase. Seventy-two hour fasting has a modifying role. All disaccharidase activities tended to decrease, except for maltase in the ANIT treated group, where an increase was recorded. gamma-GT showed no significant changes after either GALN or ANIT treatment in rats fasted 24 h. However, the 72-hour food deprivation diminished it in ANIT intoxication. It is obvious that the intestinal enzymes are influenced by the hepatic damage produced by GALN and ANIT.
D015058:1-Naphthylisothiocyanate / Q000494:pharmacology*; D015720:5'-Nucleotidase; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D005688:Galactosamine / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D009708:Nucleotidases / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013393:Sucrase / Q000097:blood; D013861:Thiocyanates / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000097:blood; D001616:beta-Galactosidase / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
D013861:Thiocyanates; D015058:1-Naphthylisothiocyanate; D005688:Galactosamine; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase; D001663:Bilirubin