Stability of polyadenylated mRNA in pig lymphocytes.
The stability of cytoplasmic poly(A)-containing mRNA in unstimulated pig lymphocytes has been determined by [3H]poly(U) hybridization after actinomycin D treatment. This approach has shown that the majority of the mRNA is much more unstable than has been suggested by earlier pulse-chase experiments, and has a half-life of between 1 and 2 h.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology*; D006207:Half-Life; D008214:Lymphocytes / Q000378:metabolism*; D011061:Poly A / Q000097:blood*; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000097:blood*; D013552:Swine
D001798:Blood Proteins; D012333:RNA, Messenger; D003609:Dactinomycin; D011061:Poly A; D012313:RNA