Sequence of the CNBr peptide containing the putative essential tyrosine of Bacillus amyloliquefaciens alpha amylase.
The amino acid sequence of the cyanogen bromide peptide (peptide B) containing the putative essential tyrosine residue in Bacillus amyloliquefaciens alpha-amylase (EC 3.2.1.1.) was determined. It is composed of 73 amino acids and the "active" tyrosine residue is the N-terminus of the peptide. Upon iodination of the whole enzyme by means of a lactoperoxidase-catalyzed reaction, a minimum of eight tyrosine residues are iodinated. Four of these belong to peptide B. Among the cyanogen bromide peptides, B is the most readily iodinated one. Hence, it is predicted that peptide B is an exposed segment of the amylase molecule.
D000595:Amino Acid Sequence; D000681:Amylases / Q000032:analysis*; D001407:Bacillus / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide; D010446:Peptide Fragments / Q000032:analysis; D014443:Tyrosine / Q000032:analysis*; D000516:alpha-Amylases / Q000032:analysis*
D010446:Peptide Fragments; D014443:Tyrosine; D000681:Amylases; D000516:alpha-Amylases; D003488:Cyanogen Bromide