Inhibition of cell-free protein and RNA syntheses by partially purified fractions of mouse melanoma extract.
Ethanol precipitate (70-95%) of the water extract of mouse melanoma (ME) contains suppressors for melanocyte cell division. A fraction which had preferential melanocyte cell line suppressor activity (ME IV2) was shown to inhibit protein synthesis by cell-free systems and RNA synthesis by isolated nuclei of rat liver. The separation and some characterization of the inhibitory factors in ME IV2 were carried out. Upon being boiled, the factor in ME IV2 inhibiting cell-free protein synthesis became inactive, whereas that inhibiting cell-free RNA synthesis remained active. Bio-Gel p-2 column chromatography of ME IV2 gave three distinct fractions (ME IV2-A, -B and -C). ME IV2-A was inhibitory to cell-free protein synthesis but non-inhibitory to cell-free RNA synthesis. On the contrary, ME IV2-B was non-inhibitory to cell-free protein synthesis but inhibitory to cell-free RNA synthesis. ME IV2-C was non-inhibitory to cell-free protein synthesis and seemed to be somewhat inhibitory to cell-free RNA synthesis. Preliminary analyses of the components in these subfractions are also reported.
D000818:Animals; D002453:Cell Cycle; D002474:Cell-Free System; D002850:Chromatography, Gel; D006358:Hot Temperature; D008545:Melanoma / Q000032:analysis*; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D014020:Tissue Extracts / Q000494:pharmacology
D014020:Tissue Extracts; D012313:RNA