A method of comparing spermatozoa with light and scanning electron microscopy.
A new method of comparing light microscopy and scanning electron microscopy in the study of small cells, such as spermatozoa, that must be examined under oil immersion is described. A grid is etched on the corner of a microscope glass slide, and its inner edges are incised. Its surface area is calculated as a function f the chamber of the critical-point drying apparatus. This method dispenses with the need for any special coverslip and enables the cells to be observed under oil immersion.
D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D008297:Male; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D013077:Sperm Head / Q000648:ultrastructure; D013082:Sperm Tail / Q000648:ultrastructure; D013094:Spermatozoa / Q000648:ultrastructure*; D013194:Staining and Labeling