T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to MHC region antigens.
The maturation sequences of thymocytes is known to some extent: A generative layer of subcapsular large lymphoblasts gives rise to a major population of small cortical thymocytes and a minor population of midsize medullary thymocytes. The relative contribution of these three populations to the peripheral T cell populations is not yet known. In this study, subcapsular lymphoblasts, cortical small cells, medullary cells, and thymic emigrant cells have all been analyzed by immunofluorescence for expression of the antigens H-2D, I-A, H-2K, and TL. H-2D is expressed brightly on all subcapsular large cells, dimly on cortical small cells, and brightly on all migrants, cortisone-resistant thymocytes (CRT), and peripheral T cells. I-A can be detected at low levels on 30 to 50% of cells in all the thymic subpopulations, and on 30 to 50% of migrants and peripheral T cells. Fifty to 80% of small cortical cells do not express detectable H-2K, but all the other subpopulations, both inside and outside the thymus, stain uniformly quite brightly. TL3 is expressed on 70 to 80% of subcapsular and cortical thymocytes, 30 to 40% of CRT, is undetectable on migrants but can be seen at low levels on 10 to 20% of spleen and lymph node T cells. The possibility that some or all of these antigens represent stable markers of separate lineages rather than unstable, stage-specific markers is discussed.
D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation; D002999:Clone Cells / Q000276:immunology; D003348:Cortisone / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D051379:Mice; D012235:Rhodamines; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology*
D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D012235:Rhodamines; D003348:Cortisone