Resistance to immune cytolysis induced by 4NQO and MNNG: a possible mechanism involved in chemical carcinogenicity.
L1210 cells were cultured for 20 hr in the presence of 4NQO or MNNG. Both agents rendered L1210 cells resistant to immune cytolysis by rabbit antibody and guinea pig complement. This resistance to immune cytolysis was not due to a loss or a distribution change of antigenic determinants to rabbit antibody, since the treated cells retained the capacity to consume guinea pig C4 in the presence of rabbit antibody to the same extent as untreated L1210 cells. Because acyltransferase activity to incorporate free fatty acids into phospholipids and neutral lipids of L1210 cells increased after the 4NQO and MNNG treatments, it was suggested that there might be a close relationship between the increased resistance of L1210 cells to immune cytolysis and the increase in their acyltransferase activity.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D003167:Complement Activation; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006168:Guinea Pigs; D007939:Leukemia L1210 / Q000139:chemically induced* / Q000276:immunology; D050356:Lipid Metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009247:NADH, NADPH Oxidoreductases / Q000494:pharmacology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits
D000939:Epitopes; D009829:Oleic Acids; D010743:Phospholipids; D008769:Methylnitronitrosoguanidine; D019301:Oleic Acid; D015112:4-Nitroquinoline-1-oxide; D009247:NADH, NADPH Oxidoreductases; C021877:nitroquinoline N-oxide reductase