title: The biosynthesis of oligosaccharide-lipids. Formation of an alpha-1,2-mannosyl-mannose linkage.
abstract: An enzyme present in rabbit liver microsomes has been found to catalyze mannosyltransfer from GDP-mannose to exogenously added oligosaccharide-lipid acceptor resulting in the formation of an alpha-1,2-mannosyl-mannose linkage. Several lines of evidence suggest that the product is a heptasaccharide containing a single 1,6-linked branched glycosyl unit with 65% of the newly added mannosyl units in a terminal nonreducing position. The enzyme has been solubilized with non-ionic detergents and was partially purified on DEAE-cellulose and hydroxylapatite. The partially purified enzyme no longer catalyzed the formation of dolichol-P-mannose indicating a direct transfer from GDP-mannose to oligosaccharide-lipid acceptor.
mesh_terms: D000818:Animals; D006017:Glycolipids / Q000096:biosynthesis*; D006155:Guanosine Diphosphate Mannose; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008364:Mannosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D009844:Oligosaccharides / Q000096:biosynthesis*; D011817:Rabbits
chemical_list: D006017:Glycolipids; D009844:Oligosaccharides; D006155:Guanosine Diphosphate Mannose; D006602:Hexosyltransferases; D008364:Mannosyltransferases