T cell clones restricted to "hybrid" HLA-D antigens?
The genetics of T lymphocyte/accessory cell interactions were studied by using antigen-specific monoclonal T cell lines from human peripheral blood and X-irradiated non-T cells (E rosette-depleted) as accessory cells. The T cell lines were obtained by expanding single colonies from soft agar-cloned E rosette-positive lymphocytes, previously incubated for 3 days in liquid culture with 30% autologous X-irradiated non-T cells and antigen (PPD). When investigated with both autologous and allogeneic accessory cells of various HLA-D genotype, different sets of PPD-specific T cell lines were obtained from HLA-D heterozygous individuals; two sets restricted to each one of the two HLA-D antigens, and a third set responding to PPD only when presented by accessory cells carrying both HLA-D antigens possessed by the T cell donor. A few cell lines responded to PPD only in the presence of autologous accessory cells.
D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014373:Tuberculin / Q000276:immunology
D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D014373:Tuberculin; D013936:Thymidine