Inhibition by ethionine and beta-diethylaminoethyl diphenylpropylacetate of RNA and protein synthesis in the rat liver, and its reversal by pregnenolone-16 alpha-carbonitrile.
The effect of pregnenolone-16 alpha-carbonitrile (PCN) on female Sprague-Dawley rats was studied in conjunction with ethionine and beta-diethylaminoethyl diphenylpropylacetate (DADP). The cyanosteroid virtually abolished in inhibitory action of ethionine on orotic acid and leucine uptake by liver microsomes. When PCN was given conjointly with DADP, it completely overshadowed the latter's effect on leucine uptake. DADP was found to act as an inhibitor of the translational mechanism 5 h after its administration, while it exerted a stimulatory influence on the transcriptional mechanism after the same time period.
D000818:Animals; D005001:Ethionine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005260:Female; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D009963:Orotic Acid / Q000378:metabolism; D011285:Pregnenolone Carbonitrile / Q000494:pharmacology*; D011335:Proadifen / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
D011285:Pregnenolone Carbonitrile; D009963:Orotic Acid; D012313:RNA; D011335:Proadifen; D007930:Leucine; D005001:Ethionine