Preparation of viable islet cells from dogs by a new method.
A new method for the preparation of viable islet cells from the normal dog pancreas is described, based on the perfusion of the pancreatic duct with collagenase. Exposure of the acinar tissue to the highest concentrations of collagenase results in improved islet yield with decreased acinar contamination. After the selective digestion of the gland by ductal perfusion, mechanical dissociation liberates single cells and clumps. Analysis of the procedure by insulin yield and amylase attrition indicates a 57% B-cell recovery and a sixfold enrichment in B-cell concentration. The cells have been transplanted as autografts into dogs after pancreatectomy. In 5 of 7 transplanted dogs, normoglycemia was achieved postoperatively.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D004285:Dogs; D006652:Histological Techniques; D007515:Islets of Langerhans / Q000166:cytology*; D013194:Staining and Labeling