Replication and expression of fragments of phage PM2 cloned in Escherichia coli K-12.
DNA from PM2 phage was cloned, as HindIII fragments and inserted into the pBR322 vector in E. coli cells. It was shown, that replication of recombined plasmids starts from the pBR322 origin. Transcription of recombinant plasmids in E. coli cells, as well as translation in minicells was demonstrated and attributed to pBR322 and/or PM2 DNA sequences.
D001435:Bacteriophages / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D014158:Transcription, Genetic; D014779:Virus Replication
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes