Influence of anions, cations and osmolarity on IgE-mediated histamine release from human basophils.
Allergen-activated human basophils require Ca2+ for the in vitro release of histamine. This study evaluated the importance of anions, cations and osmotic pressure on histamine release from human basophils. All media contained 1 mM Ca2+ and various salts or sugars were used to replace the NaCl-KCl of the control medium. Permeant anions supported release of histamine with the following order of activities: acetate - greater than Br-, I-, greater than Cl- greater than SO42-. When monovalent cations of the standard medium were replaced, RbCl, CsCl, KCl or NaCl gave results identical to the NaCl-KCl control medium; choline chloride usually enhanced while LiCl inhibited release. Finally, when sugar solutions were used to replace monovalent anions and cations of the standard medium: mannitol, lactose, sucrose, sorbitol, fructose and glucose each supported histamine release. Increasing the osmolarity of the medium by adding additional NaCl-KCl enhanced the ability of suboptimal concentrations of ragweed antigen E or anti-IgE to activate and to release histamine from leucocytes. The results indicate that histamine from leucocytes. The results indicate that histamine release from basophils requires only Ca2+ and do not support the "chemiosmotic swelling' hypothesis of secretion for these cells.
D000838:Anions / Q000494:pharmacology; D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002122:Calcium Chloride / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D066298:In Vitro Techniques; D009994:Osmolar Concentration
D000838:Anions; D002241:Carbohydrates; D002414:Cations, Monovalent; D007073:Immunoglobulin E; D002122:Calcium Chloride