A microfluorometric immunoassay for the demonstration of human AFP.
A competitive Sepharose bead immunofluorescence assay is described which allows the quantitative detection of human alpha-fetoprotein. The test is highly specific and sensitive. The smallest demonstrable concentration of AFP is about 10 ng/ml.
D006528:Carcinoma, Hepatocellular / Q000032:analysis; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D005333:Fetus / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis; D008297:Male; D010051:Ovarian Neoplasms / Q000032:analysis; D013724:Teratoma / Q000032:analysis; D013736:Testicular Neoplasms / Q000032:analysis; D014020:Tissue Extracts / Q000032:analysis; D000509:alpha-Fetoproteins / Q000032:analysis*
D014020:Tissue Extracts; D000509:alpha-Fetoproteins