Analytical electron microscopic evaluation of the effects of paraformaldehyde pretreatment on the reaction of glutaraldehyde with biogenic amines.
Analytical electron microscopy was used to determine the quantitative effects of paraformaldehyde pretreatment on the formation of the biogenic amine-glutaraldehyde-chrome complex. Pretreatment with paraformaldehyde prevented the glutaraldehyde-chrome reaction with norepinephrine in the rat adrenal medulla. In contrast to the effect of paraformaldehyde on norepinephrine, pretreatment did not prevent the chrome reaction in serotonin-containing argentaffin cells of the gut. X-Ray energy spectrographic analysis revealed a significant decrease in chrome content in the paraformaldehyde treated tissue, but sufficient chrome did react to produce an electron-dense product. Thus by treating tissue with paraformaldehyde prior to the glutaraldehyde chrome procedure, serotonergic sites may be differentiated from catecholaminergic areas at the electron microscopic level.
D000313:Adrenal Medulla / Q000648:ultrastructure*; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D004837:Epinephrine / Q000032:analysis; D005557:Formaldehyde; D005976:Glutaral; D007583:Jejunum / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009638:Norepinephrine / Q000032:analysis; D011108:Polymers; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis; D013194:Staining and Labeling
D001679:Biogenic Amines; D011108:Polymers; D005557:Formaldehyde; D012701:Serotonin; D005976:Glutaral; D009638:Norepinephrine; C003043:paraform; D004837:Epinephrine