In situ demonstration of DNA with Schiff-type dyes prepared with meta-phosphoric or tartaric acid.
A new method for the preparation of azure A-SO2 and safranine-SO2 For use in Feulgen procedure has been described herein. The method involves the use of m-phosphoric acid or tartaric acid in place of N HCl in the preparation of these eye-reagents which exhibit enhanced pH producing increased staining intensity of the nuclei as compared with those of the controls, prepared with N HCl. Possible explanation for the increased staining intensity as well as the reason for the shorter shelf-life of these eye-reagents have been offered.
D000818:Animals; D001399:Azure Stains; D002467:Cell Nucleus / Q000032:analysis; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D010619:Phenazines; D010756:Phosphoric Acids; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D013644:Tartrates
D001399:Azure Stains; D004396:Coloring Agents; D010619:Phenazines; D010756:Phosphoric Acids; D013644:Tartrates; D004247:DNA; C009195:safranine T