Isolation and structural characterization of the equine erythrocyte receptor for enterotoxigenic Escherichia coli K99 fimbrial adhesin.
The erythrocyte receptor for Escherichia coli K99 fimbrial adhesin was isolated from equine erythrocytes and characterized as Neu5Gc-alpha(2----3)-Galp-beta(1----4)-GLcp-beta(1----1)-Ceramide. This glycolipid acted as the receptor for K99 by four different experimental approaches: inhibition of equine erythrocyte hemagglutination by preincubation of K99-positive bacteria or purified K99 fimbriae with the isolated glycolipid; inhibition of attachment of K99-positive bacteria to porcine intestinal epithelial cells in the presence of the isolated glycolipid; induction of binding of K99-positive bacteria or purified K99 fimbriae to normally unreactive guinea pig erythrocytes by coating these cells with the isolated glycolipid; and isolation of the receptor by affinity chromatography with K99 coupled to CNBr-activated Sepharose 4B, indicating a strong interaction between K99 and the isolated glycolipid.
D018830:Adhesins, Escherichia coli; D000268:Adhesiveness; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002240:Carbohydrate Sequence; D004910:Erythrocyte Membrane / Q000032:analysis*; D004926:Escherichia coli / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000276:immunology* / Q000378:metabolism; D006017:Glycolipids / Q000302:isolation & purification; D006162:Guanylate Cyclase; D006168:Guinea Pigs; D006384:Hemagglutination; D006736:Horses / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D011971:Receptors, Immunologic / Q000302:isolation & purification* / Q000378:metabolism; D018000:Receptors, Peptide
D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D006017:Glycolipids; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled