Uridine uptake inhibition in Novikoff hepatoma cells by perturbants of intracellular Ca2+ and K+ levels.
The rate of uptake of uridine into the acid-soluble fraction of Novikoff hepatoma cells is inhibited by low concentrations of the ionophores A23187 and gramicidin and other perturbants of intracellular cation levels. Inhibition of uridine uptake by A23187 is dependent on Ca2+ and is reduced by serum and high levels of Mg2+. The effectiveness of A23187 is dependent on the Ca2+/Mg2+ ratio rather than the absolute concentration of either ion. Inhibition of uridine uptake by gramicidin is not significantly affected by serum or divalent cations. Other effectors of monovalent cation flux such as ouabain and valinomycin also inhibit uridine uptake. These results indicate that net uptake of uridine may be influenced by intracellular levels of certain monovalent and divalent inorganic cations.
D000818:Animals; D001769:Blood; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006096:Gramicidin / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D014529:Uridine / Q000037:antagonists & inhibitors / Q000378:metabolism*
D006096:Gramicidin; D000001:Calcimycin; D008274:Magnesium; D011188:Potassium; D002118:Calcium; D014529:Uridine