Hoechst 33258 induced uncondensed sites in marsupial chromosomes.
The fluorochrome Hoechst 33258 induces pronounced uncondensed regions at mitosis at one or more specific sites on the X chromosomes of all eighteen species of marsupials belonging to the family Macropodidae which have been examined. The Y chromosomes of nearly all of these species also show sensitive sites. Autosomal regions which respond to this chemical were observed in only five species and there is evidence of polymorphism for two of these. The regions which respond usually show C-banding, but not all C-banding regions are affected. No specific effect was found in the chromosomes of eleven other species examined which are representative of 5 different Australian marsupial families. The implications of the apparent restriction of sex chromosome sensitive sites to macropods are discussed.
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D006690:Bisbenzimidazole / Q000494:pharmacology*; D002478:Cells, Cultured; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects; D005260:Female; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008394:Marsupialia / Q000235:genetics*; D008938:Mitosis / Q000187:drug effects; D012730:Sex Chromosomes / Q000187:drug effects*; D013997:Time Factors; D014960:X Chromosome / Q000187:drug effects*; D014998:Y Chromosome / Q000187:drug effects*
D001562:Benzimidazoles; D006690:Bisbenzimidazole