Synthesis of a coupled tumor antigen with specificity against transplanted tumors in C3H/HeJ mice.
A spontaneous mammary adenocarcinoma from a female C3H/HeJ mouse was excised, dissected into 2 mm3 pieces, and transplanted into 20 4-month-old C3H/HeJ female mice. After each tumor developed to approximately 1 cm3, they were excised, and their proteins were solubilized and extracted with hypertonic potassium chloride. The extract was purified and subsequently fractionated through a Sephadex G-200 column. The different fractions were read for absorbance on a Beckman spectrophotometer, and those fractions which represented a peak were pooled and tested for cutaneous delayed hypersensitivity on preimmunized guinea pigs. The two fractions which gave maximum hypersensitivity reactions were then coupled to human gamma-globulin. The conjugate was emulsified in Freund's complete adjuvant and used as a treatment against the proliferation of tumors transplanted in C3H/HeJ mice. 70 mice were used in this study. Animals treated with the coupled antigen had significantly smaller tumors when compared with nontreated animals.
D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage*; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007167:Immunotherapy; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D012882:Skin Tests; D014184:Transplantation, Homologous; D005719:gamma-Globulins / Q000008:administration & dosage
D000951:Antigens, Neoplasm; D000939:Epitopes; D005719:gamma-Globulins