Rapid separation of enkephalins and endorphins on Sep-Pak reverse phase cartridges.
A simple, rapid method is described for separating neuropeptides. Samples were injected on Sep-Pak disposable reverse phase cartridges and eluted with a step gradient of n-propanol in formate-pyridine buffer. Enkephalins are separated from metabolic products. Separation alpha, beta and gamma-endorphin demonstrated, and Met- and Leu-enkephalin are also separated. This inexpensive, reproducible method a useful in studies of neuropeptide metabolism, purification of synthesized peptides, separation prior to RIA, or concentration of neuropeptides.
D002845:Chromatography / Q000379:methods*; D004723:Endorphins / Q000302:isolation & purification*; D004743:Enkephalin, Leucine; D004744:Enkephalin, Methionine; D004745:Enkephalins / Q000302:isolation & purification*; D018822:alpha-Endorphin; D001615:beta-Endorphin; D018823:gamma-Endorphin
D004723:Endorphins; D004745:Enkephalins; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D001615:beta-Endorphin; D018822:alpha-Endorphin; D018823:gamma-Endorphin