Cloning and characterization of cDNA sequences corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin.
A library of cDNA clones was constructed from adult rat skeletal muscle mRNA, from which a set of contractile protein clones was selected. These clones were identified by sequencing the cDNA inserts and comparing the derived amino acid sequences with published sequences of rabbit contractile proteins. In this manner, clones corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin were identified. A high degree of amino acid sequence conservation was found upon comparison of the rat and rabbit proteins. Using the cDNA clone panel, we analyzed the expression of abundant rat muscle mRNAs. We show that abundant rat muscle mRNAs can be classified into four developmentally regulated groups, based upon their expression at different stages of myogenesis. One class of mRNAs is expressed during all stages of muscle development. Since these mRNAs are also present in nonmuscle tissues, we conclude that they code for housekeeping proteins. The second class of mRNAs is present in both embryonic and adult muscle, while a third class of mRNAs is expressed only in adult muscle. A small number of mRNAs, which are present at greater levels in undifferentiated myoblasts than in adult muscle, comprise a fourth class. These results suggest the existence of at least four modes of gene control during myogenesis.
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000378:metabolism; D009218:Myosins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012313:RNA / Q000235:genetics; D051381:Rats; D014335:Tropomyosin / Q000235:genetics*; D014336:Troponin / Q000235:genetics*; D019209:Troponin C; D020107:Troponin T
D000199:Actins; D004274:DNA, Recombinant; D009124:Muscle Proteins; D014335:Tropomyosin; D014336:Troponin; D019209:Troponin C; D020107:Troponin T; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes; D009218:Myosins