Morphology and cytochemistry of bovine bone marrow mononuclear phagocytes.
Bone marrow samples were collected from five normal calves and mononuclear cells were separated using Ficoll-Hypaque. Mononuclear cells were cultured on coverslips in Leighton tubes for six hours. The adherent cells were differentiated using Wright's and nonspecific esterase stains. Monoblasts, promonocytes and monocytes were present in the proportion of 1:2.31:4.96.
D000818:Animals; D001707:Biopsy, Needle / Q000662:veterinary; D001854:Bone Marrow Cells; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002417:Cattle / Q000033:anatomy & histology*; D046508:Culture Techniques; D008297:Male; D009000:Monocytes / Q000145:classification / Q000166:cytology* / Q000201:enzymology; D013194:Staining and Labeling / Q000379:methods
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase