[Experimental induction of rotund bodies in Thermus equaticus].
With several isolates of T. aquaticus rotund bodies (rbs) have been artificially induced. The most active agents were lysozyme, pronase (not trypsin), cellulase (not alpha-amylase or beta-glucosidase), penicillin and some mineral salts. Two modifications of rbs were observed: the vesticular type arising from single cells or filaments and the aggregate-rbs resulting from secondary associations of several cells or filaments. In both cases the primary event is a partial detachment of the outer layer of cell envelopes followed by its swelling to form a hyaline bleb. Within these osmotically stable blebs cells and/or filaments involved in the ribs production become irreversibly trapped. Appearance of filaments and rbs in stationary phase cultures are discussed as a consequence of unbalanced growth culminating in abnormal autolytic reactions.
D000681:Amylases / Q000494:pharmacology; D002480:Cellulase / Q000494:pharmacology; D008903:Minerals / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D010400:Penicillin G / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D013824:Thermus / Q000166:cytology* / Q000187:drug effects; D014357:Trypsin / Q000494:pharmacology; D001617:beta-Glucosidase / Q000494:pharmacology
D008903:Minerals; D000681:Amylases; D009113:Muramidase; D001617:beta-Glucosidase; D002480:Cellulase; D014357:Trypsin; D011402:Pronase; D010400:Penicillin G
Experimentelle Induktion von "rotund bodies" bei Thermus aquaticus.