The extraction, characterization and in vitro translation of RNA from adult Schistosoma mansoni.
We have extracted RNA from Schistosoma mansoni using the lithium chloride-urea method which gives good yields of undegraded RNA. The results of agarose gel electrophoresis of RNA extracted by this procedure suggest that S. mansoni has an in vivo nick in the large rRNA sub-unit. Translation of the RNA in a rabbit reticulocyte lysate gave significant incorporation of [35S]methionine into synthesized proteins. Immunoprecipitation of these translation products using a hyperimmune monkey serum sedimented between 5 and 8% of the radioactivity, which appeared to be present in approximately 13 proteins of molecular weights 18, 20, 21, 22, 23, 35, 40, 54, 60, 70, 74, 78 and 105 K Daltons.
D000818:Animals; D002474:Cell-Free System; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012550:Schistosoma mansoni / Q000235:genetics*
D012313:RNA