Application of rat mast cell incubates as a possible short-time test for sensitizing occupational chemicals.
The direct action of sensitizing occupational chemicals (formaldehyde, phenol, phenylhydrazine, p-aminophenol) on rat mast cells was investigated by determination of histamine using HPLC separation and fluorimetric detection. It turned out that dispensed mast cells from immunized and non-immunized Wistar-rats are more sensitive than small-cut lung tissue slices. Passive cutaneous anaphylaxis was negative after a fortnight sensitizing experiment with the here described occupational chemicals. Short-time tests with rat mast cells reflect anaphylactoid response and are suitable for the screening of sensitizing chemicals.
D000707:Anaphylaxis / Q000378:metabolism; D000818:Animals; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000209:etiology*; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects*; D009784:Occupational Diseases / Q000139:chemically induced*; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D011919:Rats, Inbred Strains; D014116:Toxicology / Q000379:methods*