Phosphorylation in vitro and in vivo of the wheat embryo RNA polymerase II.
One of the large subunits (220 000 daltons) of the wheat embryo RNA polymerase II was demonstrated to undergo phosphorylation with [gamma-32P]ATP in a reaction catalysed by a homologous protein kinase preparation. The same subunit was also observed to be phosphorylated in vivo, at the onset of germination. The phosphorylation resulted in a moderate increase of the RNA polymerase activity.
D001345:Autoradiography; D046508:Culture Techniques; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification; D012313:RNA; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology*
D010761:Phosphorus Radioisotopes; D012313:RNA; D011494:Protein Kinases; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases