Leukocyte-dependent platelet vasoactive amine release and immune complex deposition in African swine fever.
Fifteen pigs were inoculated with African swine fever virus in a study of the pathogenesis of the disease. All pigs surviving the first two weeks developed high circulating antibody titers against African swine fever virus and persistent viremia. Hemolytic complement levels declined to 50 to 70 hemolytic complement 50 (CH50) units/ml from mean preinoculation levels of 120 CH50 units/ml. Immune deposits consisting of African swine fever antigen, host immunoglobulin G, and native C3 were found in the glomeruli of surviving pigs. All pigs surviving two weeks after inoculation developed leukocyte-bound antibody functionally characteristic of immunoglobulin E (IgE). Antigen-specific degranulation of antibody-coated leukocytes produced secondary platelet aggregation and vasoactive amine release. The results suggest that the IgE-basophil-platelet loop acting via amplification by leukocyte-derived platelet-activating factor participates in the immune complex deposition process in African swine fever.
D000357:African Swine Fever / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D003165:Complement System Proteins / Q000032:analysis; D000939:Epitopes; D007962:Leukocytes / Q000502:physiology*; D008244:Lysophosphatidylcholines / Q000502:physiology; D010972:Platelet Activating Factor; D010974:Platelet Aggregation; D012701:Serotonin / Q000378:metabolism*; D013552:Swine
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000939:Epitopes; D008244:Lysophosphatidylcholines; D010972:Platelet Activating Factor; D012701:Serotonin; D003165:Complement System Proteins