title: Insulin-stimulated glucose uptake, leucine incorporation into protein, and uridine incorporation into RNA in skin fibroblast cultures from patients with diabetes mellitus.
abstract: Since intrinsic cellular abnormalities have previously have reported in diabetes mellitus, skin fibroblasts from patients with insulin-dependent diabetes and non-insulin-dependent diabetes, and from age-matched young and old controls, were examined for stimulation by insulin (4--4000 ng/ml) of glucose uptake, leucine incorporation into protein, and uridine incorporation into RNA. No differences in insulin-stimulated glucose uptake were seen between donor types. At 40 and 400 ng/ml, insulin did not stimulate as much leucine incorporation into protein in insulin-dependent diabetics as in young controls (p less than 0.05) and at 4000 ng/ml, less insulin-stimulated leucine incorporation was seen in insulin-dependent diabetics than in young controls or non-insulin dependent diabetics (p less than 0.01). Lower insulin-simulated uridine incorporation into RNA in old controls than in other cell lines appeared to be largely secondary to a two-fold increase in basal incorporation in these old controls. These results provide additional evidence for intrinsic cellular abnormalities in diabetes mellitus. Whether the differences in basal or insulin-stimulated response between fibroblasts of different donor types are attributable to alterations in protein or RNA synthesis, metabolite pool size or turnover have yet to be determined.
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
chemical_list: D007328:Insulin; D012313:RNA; D007930:Leucine; D005947:Glucose; D014529:Uridine