Lymphokine (interleukin 2) secretion as a measure of T cell recognition of parasite antigens.
The secretion of the lymphokine, Interleukin 2 (IL2), from cells obtained from the lymph nodes of mice infected by the murine nematode, Nematospiroides dubius, is used an indication of T cell recognition of parasite antigens. It is demonstrated that IL2 secretion is T cell-dependent and occurs only when the lymph node cells are cultivated in the presence of at least 5 micrograms/ml of a parasite antigen preparation. The presence of adherent peritoneal exudate cells from uninfected mice in the cultures is an essential requirement for lymphokine release. This system can be used to quantitate T cell recognition of parasite antigens and to study T cell/antigen-presenting-cell interactions involved in the immune response to parasite infection.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001202:Ascitic Fluid / Q000166:cytology; D000939:Epitopes; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D008222:Lymphokines / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009348:Nematoda / Q000276:immunology; D009349:Nematode Infections / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D007376:Interleukin-2; D008222:Lymphokines