Rapid determination of alpha-fetoprotein in amniotic fluid using an automated immunoturbidimetric technique.
A rapid and precise immunoturbidimetric assay for the measurement of alpha-fetoprotein in amniotic liquor has been developed. The method is an 'end-point' technique without blank correction since non-specific turbidity does not occur with amniotic liquor. The assay is able to distinguish 4 mg/l from zero and is unaffected by gross blood staining of the liquor. The protocol requires a duplicate analysis of the liquor to detect high levels of alpha-fetoprotein.
D000653:Amniotic Fluid / Q000032:analysis*; D000757:Anencephaly / Q000175:diagnosis; D001769:Blood; D005260:Female; D006801:Humans; D007118:Immunoassay; D007122:Immunoelectrophoresis; D007700:Kinetics; D009391:Nephelometry and Turbidimetry; D011247:Pregnancy; D016136:Spina Bifida Occulta / Q000175:diagnosis; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins