Binding of benzo [a]pyrene to epidermal DNA and RNA as detected by synchronous luminescence spectrometry at 77 K.
The fluorescence associated with benzo[a]pyrene [BP] moieties covalently attached to the nucleic acid (DNA plus RNA) isolated from the epidermis of BP-treated mice was examined at 77 K in frozen aqueous solutions by use of a photon-counting fluorimeter operating in the synchronous scanning mode. The excitation and emission wavelengths were scanned simultaneously with the monochromators set 28 nm apart. This setting coincides with the difference in wavelength between the excitation and emission maxima for the fluorescence of bound BP. Currently the level of detection is in the order of 1 BP residue per 200,000 bases in 40 microgram of nucleic acid. This amount of nucleic acid can be isolated from the skin of a single mouse. The method described here is generally useful for detecting the binding to DNA of nonradioactive carcinogenic polynuclear aromatic which might occur following the topical application to animal skin in vivo of complex hydrocarbon mixtures such as synthetic fuels and crude oils.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D003080:Cold Temperature; D004247:DNA / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D012313:RNA / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D001580:Benzopyrenes; D012313:RNA; D004247:DNA