A method for preparation of immunosorbents by direct coupling of dissociated immune complexes.
A simple and rapid method of preparing immunosorbents by use of isolated immune complexes is described. Dissociated immune complexes may be directly coupled to activated Sepharose and an immunosorbent gel with complementary molecular populations of immunospecifically purified ligands is obtained. The antigen binding capacity of insolubilized antibodies was about 50% of the antigen bound at equivalence using liquid phase precipitation. The batch technique described may be scaled up and the only limitation is the availability of the ligands. The usefulness of the technique is illustrated in two model systems.
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D000941:Antigens; D001665:Binding Sites; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007123:Immunoelectrophoresis, Two-Dimensional; D007136:Immunoglobulins; D007164:Immunosorbents; D008297:Male; D011247:Pregnancy; D011817:Rabbits; D000511:alpha-Macroglobulins
D000936:Antigen-Antibody Complex; D000941:Antigens; D007136:Immunoglobulins; D007164:Immunosorbents; D000511:alpha-Macroglobulins