Genetic damage caused by methylazoxymethanol acetate, the aglycone of cycasin. Analysis of sister-chromatid exchange frequency under in vivo conditions and in cell cultures.
To extend the information on the mutagenic effect of methylazoxymethanol (MAM) acetate, which has been established as a potent carcinogen, the frequency of sister-chromatid exchange (SCE) was determined in bone marrow cells of Sprague Dawley rats and in cultured rat lymphocytes. To get differentially stained chromatids under in vivo conditions, the rats received 5-bromodeoxyuridine adsorbed on activated charcoal intraperitoneally. Chromosomes were stained with fluorescence plus Giemsa. In this study a significant increase of SCE frequency after the application of various concentrations of MAM acetate could be observed both in vivo and in vivo.
D000818:Animals; D001391:Azo Compounds / Q000009:adverse effects*; D001853:Bone Marrow / Q000648:ultrastructure; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003492:Cycasin / Q000032:analysis; D008214:Lymphocytes / Q000648:ultrastructure; D008746:Methylazoxymethanol Acetate / Q000009:adverse effects*; D009153:Mutagens; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013194:Staining and Labeling
D001391:Azo Compounds; D009153:Mutagens; D008746:Methylazoxymethanol Acetate; D003492:Cycasin