Immunofluorescent localization of vimentin, prekeratin and actin during odontoblast and ameloblast differentiation.
The localization of constitutive proteins of different types of cytoskeletal components (prekeratin, vimentin, and actin) was examined in embryonic mouse molars using specific antibodies and immunofluorescence microscopy on frozen sections. Prekeratin and actin were found in the enamel organ. Preameloblasts demonstrated uniform staining, whereas ameloblasts demonstrated an apical accumulation of both prekeratin and actin. Vimentin and actin were observed in the dental papilla. A redistribution of vimentin accompanied the polarization of odontoblasts. A possible transmembranous control of cytoskeletal activities by the extracellular matrix is discussed.
D000199:Actins / Q000032:analysis*; D000565:Ameloblasts / Q000166:cytology / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D005455:Fluorescent Antibody Technique; D007633:Keratins / Q000032:analysis*; D051379:Mice; D008963:Molar / Q000196:embryology; D009124:Muscle Proteins / Q000032:analysis*; D009804:Odontoblasts / Q000166:cytology / Q000502:physiology*; D009924:Organ Culture Techniques; D011247:Pregnancy; D011498:Protein Precursors / Q000032:analysis*; D014746:Vimentin
D000199:Actins; D009124:Muscle Proteins; D011498:Protein Precursors; D014746:Vimentin; C004818:prekeratin; D007633:Keratins