Characterization of nuclear non-histone proteins from rat liver isolated in the polyethylene glycol-dextran system and separated by hydroxyapatite chromatography.
1. Three groups of non-histone proteins were obtained from rat liver nuclei by separation of nucleic acids with the polyethylene glycol - dextran polymer mixture and fractionation of nuclear proteins on hydroxyapatite in the salt - glycerol - phenylmethylsulphonyl fluoride system at increasing concentration of Na+ and K+ phosphate buffer, pH 6.8. 2. Non-histone proteins of rat liver represent a heterogeneous complex of polypeptides devoid of DNA, of molecular weight ranging from 12000 to 130000, and isoelectric points from pH 4.0 to 7.8.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002845:Chromatography; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D003911:Dextrans / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007525:Isoelectric Focusing / Q000379:methods; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats
D002868:Chromosomal Proteins, Non-Histone; D003911:Dextrans; D006882:Hydroxyapatites; D011092:Polyethylene Glycols