title: Interferon (type-alpha and gamma) production by fresh and cryopreserved human mononuclear cells. Brief report.
abstract: A comparative study of interferon (IFN) production (type-alpha and gamma) was carried out using Ficoll-hypaque purified fresh and cryopreserved mononuclear cells from eight normal healthy individuals. Newcastle disease virus-NDV (R2B strain) was used as an inducer for type-alpha and Staphylococcal enterotoxin-A-(SEA) for type-gamma IFN production. There was no significant difference between the titres of type-alpha and gamma-IFN and lymphocyte subpopulations of fresh and cryopreserved mononuclear cells studied under identical conditions.
mesh_terms: D000328:Adult; D001793:Blood Preservation / Q000379:methods; D002478:Cells, Cultured; D004768:Enterotoxins; D005615:Freezing; D006801:Humans; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D009522:Newcastle disease virus; D013210:Staphylococcus
chemical_list: D004768:Enterotoxins; D007369:Interferon Inducers; D007372:Interferons