[Morphological identification of pituitary hormones by the aid of pH-determination using microphotometry (author's transl)].
The identification of pituitary ACTH and TSH producing cells was performed by histological staining at the isoelectric points (pH levels: 4.6 and 5.2 respectively) of these hormones in cat. Intensity of stained material in the hormone producing cells was determined by microphotometric technique. By the application of hormones influencing the pituitary ACTH and TSH producing relevant changes in pituitary cells could be detected.
D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D000818:Animals; D002415:Cats; D006728:Hormones / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010783:Photometry / Q000379:methods; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling; D013965:Thyroidectomy; D013972:Thyrotropin / Q000096:biosynthesis*
D006728:Hormones; D000324:Adrenocorticotropic Hormone; D013972:Thyrotropin
Morphologische Identifizierung der Hypophysenvorderlappen-Hormone durch mikrophotometrische Messungen unter Berücksichtigung der isoelektrischen Punkte der Hormone.