Isolation and characterization of complementary deoxyribonucleic acid complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid from oocytes of Drosophila melanogaster.
The complementary deoxyribonucleic acid (cDNA) complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid [poly(A+) RNA] from Drosophila melanogaster oocytes has been isolated and characterized. Analysis of the kinetics of hybridization of this cDNA (cDNAHA) to total poly(A+) RNA of oocytes indicates this class contains approximately 86 different sequences. Hybridization kinetics of cDNAHA annealed to poly(A+) RNA from 19-old embryos is essentially the same as that of oocyte poly(A+) RNA. This suggests the highly abundant class of poly(A+) RNA persists in approximately the same frequency through early development. Analysis of the hybridization of cDNAHA to genomic DNA suggests that the highly abundant poly(A+) RNA from oocytes is not enriched for transcripts from repetitive sequences of the genome.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D004331:Drosophila melanogaster; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000032:analysis; D011061:Poly A / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA