Trypsin-induced activation of the thiol esters in alpha 2-macroglobulin generates a short-lived intermediate ('nascent' alpha 2-M) that can react rapidly to incorporate not only methylamine or putrescine but also proteins lacking proteinase activity.
D000818:Animals; D002417:Cattle; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014107:Tosyllysine Chloromethyl Ketone / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology*; D000511:alpha-Macroglobulins / Q000378:metabolism*
D007328:Insulin; D008744:Methylamines; D013438:Sulfhydryl Compounds; D000511:alpha-Macroglobulins; D014107:Tosyllysine Chloromethyl Ketone; C027451:methylamine; D014357:Trypsin; D011700:Putrescine