Effects of 1,1'-hexamethylene-bis-[(5-p-chlorophenyl)-biguanide] on the genome and on the synthesis of nucleic acids and proteins in the bacterial cells.
The strongly effective bactericidal compound 1,1'-hexamethylene-bis-[(5-p-chlorophenyl)-biguanide] (HCG), which is used as a disinfectant alterates the DNA of B. subtilis as shown in the rec assay, induced auxotrophic mutants in E. coli B and causes prophage induction in Micrococcus lysodeikticus 53-40 (N5). In vivo experiments with E. coli B have demonstrated that HCG extensively breaks down bacterial DNA and interacts with the synthesis of cellular DNA to the similar extent as found for N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The structural integrity of ribosomes and of ribosomal subunits remains intact in the presence of HCG.
D001412:Bacillus subtilis / Q000187:drug effects; D001426:Bacterial Proteins / Q000096:biosynthesis; D001645:Biguanides / Q000633:toxicity*; D002710:Chlorhexidine; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects*; D008837:Micrococcus / Q000187:drug effects; D009153:Mutagens; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D011995:Recombination, Genetic / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects
D001426:Bacterial Proteins; D001645:Biguanides; D004269:DNA, Bacterial; D009153:Mutagens; D012329:RNA, Bacterial; D002710:Chlorhexidine