Effect of mouse type I interferon on mouse bone marrow cells and peritoneal exudate cells cultured in vitro.
Type I mouse interferon significantly reduces macrophage colony formation from mouse bone marrow cells or mouse peritoneal exudate cells when cultured in vitro in the presence of colony-stimulating factors (CSF) derived from either pregnant mouse uterus (CSFPMUE or LCM (CSFLCM) (concanavalin-A purified preparation from endotoxin-shocked mouse long-conditioned medium). The effect of interferon on granulocyte (G-CFU) or granulocyte-macrophage-mixed (GM-CFU) colonies depended on the CSF used. G-CFU and GM-CFU were somewhat elevated in interferon-treated cells stimulated by CSFPMUE whereas G-CFU and GM-CFU were slightly inhibited in interferon-treated cells stimulated by CSFLCM. The most dramatic effect of interferon was observed in the macrophage population. We conclude that interferon affects differentiation preferentially at the macrophage progenitor cell level.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis / Q000187:drug effects*; D007372:Interferons / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy
D003115:Colony-Stimulating Factors; D007372:Interferons