[Feasibility of using human spleen cells to produce interferon].
Spleen cells of subjects who died suddenly are capable of producing large amounts of interferon in response to induction with Newcastle disease virus (NDV) and Sendai virus (1-2.5 IU per 1000 cells). The optimal conditions for interferon production by these cells include using NDV, the H strain, as an inducer in a dose of 10 ID50/cell and fresh spleen cells stored no more than 24 hours at 4-6 degrees C in a concentration of 0.5-1.0 x 10(7)/ml, with the incubation time of 20-24 hours. It is recommended that spleen cells from subjects who died suddenly be used for human interferon production.
D003080:Cold Temperature; D006801:Humans; D066298:In Vitro Techniques; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D009522:Newcastle disease virus; D010222:Parainfluenza Virus 1, Human; D013154:Spleen / Q000378:metabolism*; D014021:Tissue Preservation / Q000379:methods
D007369:Interferon Inducers; D007372:Interferons
Vozmozhnost' ispol'zovaniia kletok selezenki liudeĭ dlia produktsii interferona.