Serum alphafetoprotein determination by enzyme-linked immunosorbent assay.
Enzyme-linked immunosorbent assay (ELISA) in microtitre trays has been adapted to the determination of alpha-fetoprotein (AFP); the method is described here. This assay has a lower limit of sensitivity of AFP 1 microgram/l and results compare well with those obtained with a commercial radio-immunoassay (RIA) kit (r = 0,994; y = 949x-3,00). ELISA has several advantages over RIA; it is considerably less expensive, the reagents are stable for longer periods and no elaborate equipment is required.
D003365:Costs and Cost Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008433:Mathematics; D008722:Methods; D011247:Pregnancy; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling; D000509:alpha-Fetoproteins / Q000032:analysis*
D011933:Reagent Kits, Diagnostic; D000509:alpha-Fetoproteins