Clonogenic assay and in vitro chemosensitivity testing of human urologic malignancies.
Over the past year, we have attempted to grow both primary and metastatic urologic malignancies using a recently developed human tumor cloning system. Formation of colonies in vitro occurred in 125 of 164 primary tumors (76%), including 34 of 47 uroepithelial cancer specimens, 45 of 50 renal cell cancer specimens, 24 of 33 prostatic cancer specimens, and 22 of 34 testicular cancer specimens. A large percentage of metastatic cancers have also been successfully cultured. Growth sufficient for chemosensitivity testing ranged from 43% of the uroepithelial cancers cultured to 64% of the renal cell cancer specimens cultured. When in vitro chemosensitivity testing was performed, the in vitro chemosensitivity results show a striking similarity to clinical response rates for the same agents used for these tumors. Overall the human tumor cloning system appears to be a reasonable model for the study of human urologic malignancies.
D000135:Acid Phosphatase / Q000032:analysis; D000362:Agar; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002452:Cell Count; D006063:Chorionic Gonadotropin / Q000032:analysis; D018997:Chorionic Gonadotropin, beta Subunit, Human; D002999:Clone Cells; D003584:Cytological Techniques; D004351:Drug Resistance; D005834:Genital Neoplasms, Male / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000473:pathology; D010446:Peptide Fragments / Q000032:analysis; D014571:Urologic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D000509:alpha-Fetoproteins / Q000032:analysis
D000970:Antineoplastic Agents; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; D000509:alpha-Fetoproteins; D000362:Agar; D000135:Acid Phosphatase