title: Kunitz-type proteinase inhibitors derived by limited proteolysis of the inter-alpha-trypsin inhibitor. VI. Detection of a complex between immunoglobulin g and the inhibitory active part of the inter-alpha-trypsin inhibitor.
abstract: A latent trypsin inhibitor is released from denatured human serum proteins by proteolytic digestion with thermolysin. The latent inhibitor was enriched by chromatography on DEAE-Sephacel, Sephadex G-200, and Protein A-Sepharose, respectively. Immunological cross-section identified the latent inhibitor as a complex between IgG and the inhibitory active part of the inter-alpha-trypsin inhibitor.
mesh_terms: D000510:Alpha-Globulins; D000936:Antigen-Antibody Complex; D002846:Chromatography, Affinity; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D013820:Thermolysin; D014361:Trypsin Inhibitors
chemical_list: D000510:Alpha-Globulins; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor; D013820:Thermolysin