Studies on antitumor activity of prumycin. III. Mode of action of prumycin on HeLa S-3 cells.
The mode of action of prumycin was investigated using synchronized and asynchronous cultured HeLa S-3 cells. Prumycin inhibited significantly the growth of HeLa S-3 cells at the concentration over 5 mcg/ml. DNA synthesis as well as protein synthesis was strongly inhibited at the concentration of 10 mcg/ml of prumycin, but RNA synthesis was not inhibited by the same concentration. Prumycin did not block the transition of the cells from M phase to G1 phase, however, G2 phase cells were blocked clearly by this antibiotic.
D000606:Amino Sugars / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001089:Arabinose / Q000031:analogs & derivatives / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008937:Mitomycins / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013997:Time Factors
D000606:Amino Sugars; D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; C025517:prumycin; D012313:RNA; D004247:DNA; D001089:Arabinose