Beta-thromboglobulin radioimmunoassay. A laboratory characterization and evaluation.
Platelets release beta-thromboglobulin from alpha-granules when they are activated by various stimuli. An evaluation and optimization of a radioimmunoassay for beta-thromboglobulin is described. The optimum conditions for the reaction have been characterized, and the use of second antibody and polyethylene glycol allows completion of the assay within 24 hours. Similar BTG concentrations were obtained using a 1-hour non-equilibration assay but the 1-hour assay was inefficient for processing large volumes of specimens and has the potential for cross reactivity. BTG standards were unstable but the shelf-life was prolonged with aprotinin or by storage at -70 degrees C. Plasma BTG concentration in 80 normal individuals was 28 +/- 18 ng/ml. (mean +/- 2 S.D.).
D000328:Adult; D000818:Animals; D000906:Antibodies; D001609:Beta-Globulins / Q000276:immunology*; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D005260:Female; D006041:Goats; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000494:pharmacology; D011309:Preservation, Biological; D011817:Rabbits; D011863:Radioimmunoassay; D013696:Temperature; D001620:beta-Thromboglobulin / Q000276:immunology*
D000906:Antibodies; D001609:Beta-Globulins; D001620:beta-Thromboglobulin; D011092:Polyethylene Glycols; D006493:Heparin