title: In vitro cooperation of a antigen-specific T cell-derived helper factor, B cells, and adherent cells or their secretory product in a primary IgM response to chicken MHC antigens.
abstract: The primary in vitro IgM response to the major histocompatibility (MHC) antigens expressed on chicken red blood cells (CRBC) has been shown to critically depend on the cooperation between antigen-specific helper factor from CRBC-primed thymus-derived helper (T) cells, bone marrow-derived (B) cells, and radioresistant (A) cells. A 500- to 1000-fold enrichment for immunocompetent B cells by means of a rosette technique permitted the culturing of as few as 2000 B cells that yielded on the average 500 plaque-forming cells against CRBC within 3 days of culture. Cell reconstitution experiments clearly showed that the presence of A cells can be circumvented by an A cell-derived secretory product. This is in disagreement with the hypothesis that helper factor acts as a mediator for antigen presentation on the A cell surface membrane. The spectrum of helper factor cross-reactivity with respect to certain alleles of the B locus was found comparable to that of antibody.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion; D002645:Chickens; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006649:Histocompatibility Antigens; D007075:Immunoglobulin M / Q000096:biosynthesis; D008211:Lymphocyte Cooperation; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000939:Epitopes; D006649:Histocompatibility Antigens; D007075:Immunoglobulin M