Interferon increases the abundance of submembranous microfilaments in HeLa-S3 cells in suspension culture.
Human beta (fibroblast) interferon inhibits the proliferation of human HeLa-S3 carcinoma cells in suspension culture. Accompanying this effect, the lateral mobility of cell surface receptors for concanavalin A is decreased and the rigidity of the plasma membrane lipid bilayer is increased. The present findings show a marked increase in the number of polymerized actin-containing microfilaments 3 days after treatment of HeLa-S3 cells with beta-interferon (640 units/ml). The cortical region of the treated enlarged cells contains a thick and dense meshwork of 40-70 A microfilaments. The actin nature of the filaments was verified by their ability to bind heavy meromyosin. These results support the concept that beta-interferon induces a coordinated response in the plasma membrane and the underlying microfilaments in both tumor and normal cells.
D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D015879:Myosin Subfragments / Q000032:analysis; D011070:Poly I-C / Q000494:pharmacology
D015879:Myosin Subfragments; D007372:Interferons; D011070:Poly I-C