Sleep during neuromuscular blockade in cats.
The purpose of the experiment was to determine whether normal sleep patterns can occur during neuromuscular blockade. Electrographic variables for determining the states of sleep and wakefulness, the electrocorticogram, lateral geniculate nucleus potentials, and dorsal hippocampal potentials, were recorded before, during and after the administration of gallamine triethiodide to cats with chronically implanted electrodes. When respiratory muscles became paralyzed, artificial ventilation commenced through a chronic tracheal fistula. The electrographic wave forms of the states (wakefulness, NREM sleep and REM sleep) in paralyzed cats were indistinquishable by visual observation from those of freely moving animals. As compared to freely moving cats, paralyzed cats had more wakefulness at the expense of both states of sleep (about 33% NREM and 3% REM compared to 45% NREM and 15% REM respectively). REM sleep wasdemonstrated to occur, albeit increase across repeated session in the same cats nor was the distribution uneven within the average session. Large percentages of REM sleep with respect to total recording time were associated with large percentages of NREM sleep (correlation coefficient = 0.58). The sequence of sleep states was like that of freely behaving animals. The main conclusion is that this preparation, depsite low amounts of REM sleep, is useful in neural studies of sleep and wakefulness.
D000818:Animals; D002415:Cats; D002490:Central Nervous System / Q000187:drug effects; D002540:Cerebral Cortex / Q000502:physiology; D003472:Curare / Q000494:pharmacology; D005703:Gallamine Triethiodide / Q000494:pharmacology; D009466:Neuromuscular Blocking Agents / Q000494:pharmacology*; D012890:Sleep / Q000187:drug effects / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology; D014851:Wakefulness / Q000502:physiology
D009466:Neuromuscular Blocking Agents; D003472:Curare; D005703:Gallamine Triethiodide