[Identification of isolated metaphase chromosomes. II. A comparison with the recognizability of chromosomes in metaphase plates].
The metaphase chromosomes (MC) isolated from the Chinese hamster cells were identified with the aid of differential staining (G-bands). It was shown that differences in the relative recognizability of MC in metaphase plates and after their isolation are determined by changes in composition of isolated MC, rather than by those in staining capacity of MC after their isolation. The frequencies of identified MC are constant and independent upon the type of MC preparations and relation between identified and unidentified MC in certain preparations. At allows to apply the described method for the analysis of chromosome fractionation, using changes in frequencies of identified MC as a criterion of efficiency of the fractionation method. Possible ways of increasing the recognizability level of isolated MC are discussed.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D007621:Karyotyping / Q000379:methods; D008677:Metaphase; D013194:Staining and Labeling / Q000379:methods
Identifikatsiia izolirovannykh metafaznykh khromosom. II. Sravnenie s uzhavaemost'iu khromosom v sostave metafaznykh plastinok.