Cytochemical method of detecting DNA based on reduction of metallic silver from solution of silver ammine by aldehyde groups of apurinic DNA.
A highly specific and readily reproducible cytochemical method for detecting DNA in nuclear structures of fixed cells has been developed. The method involves the reduction of metallic silver by aldehydes of the 2-deoxyribose of apurinic DNA from a solution of silver ammine prepared with allowance made for the requirements of the chemistry of the reaction. It was found that the method developed can be used for a quantitative analysis of DNA in animal cells.
D000447:Aldehydes / Q000032:analysis; D000818:Animals; D001080:Apurinic Acid / Q000032:analysis*; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis*; D002645:Chickens; D004247:DNA / Q000032:analysis*; D006168:Guinea Pigs; D008099:Liver / Q000166:cytology; D008297:Male; D011119:Polynucleotides / Q000032:analysis*; D011895:Rana ridibunda; D012708:Sertoli Cells / Q000166:cytology; D012834:Silver; D013094:Spermatozoa / Q000166:cytology; D013194:Staining and Labeling
D000447:Aldehydes; D011119:Polynucleotides; D001080:Apurinic Acid; D012834:Silver; D004247:DNA