The synthesis and isolation of DNA complementary to histone mRNA from mouse embryos.
A 9S poly(A)-RNA preparation isolated from mouse embryos was shown to stimulate the synthesis of histones in an ascites cell free extract. This RNA preparation was used for the synthesis of a highly labelled cDNA probe complementary to histone mRNA. Hybridisation of this cDNA probe to rRNA showed that 52% of the cDNA consisted of sequences complementary to rRNA. The histone mRNA specific cDNA was purified by hybridising the impure cDNA to rRNA followed by removal of the single-stranded histone cDNA by hydroxylapatite chromatography.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D006657:Histones / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014158:Transcription, Genetic
D006657:Histones; D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase