title: Dietary effects on pancreatic exocrine function. Experiments on the isolated perfused rat pancreas.
abstract: Studies of the response of the isolated perfused rat pancreas to three doses of secretin (5.0, 10.0, 18.75 U/h) and CCK (0.6, 1.5, 2.4 U/h) show that a 35-day administration of a carbohydrate-rich (60% carbohydrate, 3% fat, 20% protein) or fat-rich (34% fat, 11.3% carbohydrate, 29.8% protein) diet increases the sensitivity of the gland to these hormones compared to a standard diet (50.5% carbohydrate, 4% fat, 19% protein). The maximal secretory flow on stimulation by secretin and on a combined stimulation by secretin (2.5 U/h) plus CCK (1.8 U/h) is significantly (p less than 0.05 and p less than 0.01, respectively) lower in both experimental diets than in controls, whereas the maximal protein and enzyme secretion is diminished only in the secretin plus CCK-stimulated pancreas of rats fed a fat-rich diet. A carbohydrate-rich diet results in a relative increase in amylase secretion and decrease in lipase and chymotrypsinogen output. Compared to controls, the fat-rich diet leads to a stronger secretion of lipase and chymotrypsinogen and to a reduced amylase secretion. This adaptation of the exocrine pancreatic function is rather due to changes in the organ itself than to an altered hormonal stimulation.
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D002919:Chymotrypsinogen / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D008049:Lipase / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000201:enzymology / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012633:Secretin / Q000494:pharmacology*
chemical_list: D004040:Dietary Carbohydrates; D011506:Proteins; D012633:Secretin; D002766:Cholecystokinin; D002919:Chymotrypsinogen; D008049:Lipase; D000681:Amylases