Purification of canine myocardial mitochondrial creatine kinase.
Mitochondrial creatine kinase, purified for the first time, is a dimeric molecule with a mol. wt of 82,000 and does not hybridize with M or B subunits or react to their specific antiserum. A specific antiserum to the mitochondrial form was developed which does not cross-react with the B or M subunits. Thus, the mitochondrial form is biochemically and immunologically unique.
D000818:Animals; D003402:Creatine Kinase / Q000276:immunology / Q000302:isolation & purification*; D003600:Cytosol / Q000201:enzymology; D004285:Dogs; D000939:Epitopes; D007527:Isoenzymes; D008929:Mitochondria, Heart / Q000201:enzymology*; D008970:Molecular Weight; D011487:Protein Conformation
D000939:Epitopes; D007527:Isoenzymes; D003402:Creatine Kinase