Preparation and oxygen binding properties of soluble covalent hemoglobin-dextran conjugates.
Stroma-free hemoglobin solutions present some drawbacks when used as blood substitutes, essentially because the hemoprotein has a low vascular retention, due to its small hydrodynamic volume. Covalent coupling of the protein with dextran derivatives artificially increases its size and affords polymeric conjugates whose oxygen-binding properties (Barcroft's curve, Hill coefficient) depend on the molecular weight.
D055598:Chemical Phenomena; D002621:Chemistry; D003911:Dextrans; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*
D003911:Dextrans; D006454:Hemoglobins; D010100:Oxygen