title: Altered expression of Plasmodium knowlesi variant antigen on the erythrocyte membrane in splenectomized rhesus monkeys.
abstract: Variant antigens are present on the surface of Plasmodium knowlesi malaria-infected erythrocytes as detected by the schizont-infected cell agglutination (SICA) assay. We found that parasitized erythrocytes passaged in splenectomized monkeys did not agglutinate with immune sera. On the first passage from intact to splenectomized monkeys, the SICA titers decreased 4- to 16-fold; after the second and subsequent passages in splenectomized monkeys, the infected cells became nonagglutinable to all sera tested, including sera from animals infected with the nonagglutinating parasites. This loss of agglutinability could have resulted from selection of a genetically distinct subpopulation of the original parasites or the ability of the original parasites to alter their phenotypic expression. We have designated the new nonagglutinable phenotype, SICA [-], and the agglutinable phenotype, SICA [+]. The loss of agglutinability indicates that the variant antigen normally expressed on the erythrocyte membrane of infected cells is altered or absent. Because SICA [-] parasites developed in the absence of the spleen, the major organ of host defense against malaria, then this organ may in some manner influence or modulate antigenic expression in P. knowlesi and possibly other malaria parasites.
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D000939:Epitopes; D004910:Erythrocyte Membrane / Q000276:immunology* / Q000469:parasitology; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology* / Q000469:parasitology; D005260:Female; D014644:Genetic Variation; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000494:pharmacology; D008253:Macaca mulatta; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D008297:Male; D010641:Phenotype; D013156:Splenectomy
chemical_list: D000941:Antigens; D000939:Epitopes; D007106:Immune Sera