A chemical method to enrich RNA by molecules having 5'-terminal triphosphate groups.
A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method is based upon selective chemical modification of 5'-triphosphate groups by an antigen-containing amine followed by affinity chromatography on an absorbent loaded with antibodies specific to this antigen. A purification factor up to 37 may be achieved.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis; D055598:Chemical Phenomena; D002621:Chemistry; D004546:Elapid Venoms; D004720:Endonucleases; D006027:Glycosides / Q000138:chemical synthesis; D007202:Indicators and Reagents; D008722:Methods; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000138:chemical synthesis*; D011865:Radioisotope Dilution Technique; D012265:Ribonucleotides; D014316:Tritium
D004546:Elapid Venoms; D006027:Glycosides; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D012265:Ribonucleotides; D014316:Tritium; C029976:4-aminophenyl beta-lactoside; C029977:ATP-gamma amidate; D012313:RNA; D000255:Adenosine Triphosphate; D004720:Endonucleases