Effect of anti-Lyb3 antiserum on poly (L-glutamic acid, L-lysine)-induced B cell tolerance.
The effect of anti-Lyb3 antiserum on antigen-specific B cell tolerance was investigated. The intraperitoneal injection of the nonimmunogenic copolymer L-glutamic acid60, L-lysine (GL) specifically reduces the ability of murine B cells to form GL-specific plaque-forming cell responses following challenge with the immunogenic conjugate of GL coupled to fowl gamma-globulin. It was found that this tolerance could be reversed or blocked by the intravenous injection of microliter quantities of anti-Lyb3 antiserum. However, this dose of antiserum neither reversed T cell tolerance induced with protein-coupled syngeneic erythrocytes nor induced tolerized B cells to secrete antibody. The results suggest that B lymphocytes can be rescued from GL-induced tolerance soon after induction and that Lyb3 determinants may play a functional role in the activation of antigen-specific B lymphocytes.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D007108:Immune Tolerance / Q000187:drug effects*; D007518:Isoantibodies; D051379:Mice; D010455:Peptides / Q000276:immunology* / Q000494:pharmacology; D011099:Polyglutamic Acid / Q000276:immunology* / Q000031:analogs & derivatives; D011107:Polylysine / Q000276:immunology* / Q000031:analogs & derivatives; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D007518:Isoantibodies; C029805:Lyt antibodies; D010455:Peptides; D011107:Polylysine; D011099:Polyglutamic Acid; C030391:poly(glutamic acid-lysine)