Propionic acid-metabisulphite-Schiff reagent for quick Feulgen staining.
This communication presents a method for the preparation of Schiff reagent in which N hydrochloric acid has been replaced by a low concentration of propionic acid. The result of using such a Schiff reagent indicates that the dye-reagent thus prepared is extrafast in action on acid-hydrolysed mammalian tissue sections. The intensity of nuclear colouration is also increased considerably, since the pH of the dye-reagent is 6.0. It is, therefore advocated that this newly developed Schiff reagent be used at 5 degrees C.
D000818:Animals; D004396:Coloring Agents; D008099:Liver / Q000032:analysis; D008297:Male; D011422:Propionates; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds; D013447:Sulfites; D013737:Testis / Q000032:analysis
D004396:Coloring Agents; C007722:Feulgen stain; D011422:Propionates; D012394:Rosaniline Dyes; D013438:Sulfhydryl Compounds; D013447:Sulfites; C005199:potassium metabisulfite; C029658:propionic acid; C005732:thioacetic acid