Measurement of DNA synthesis by flow cytometry.
Cells were stained for DNA content with Hoechst 33258 combined with propidium iodide and analyzed on an epi-fluorescence flow cytometer. Scatterplots of red versus blue fluorescence of control cells showed a straight line indicating that both dyes were measuring proportional amounts of cellular DNA. Scatterplots of cells incubated with the thymidine analog bromodeoxyuridine, however, showed a displacement from the control straight line that increased with longer exposure time to bromodeoxyuridine. These results suggest that bromodeoxyuridine incorporation in DNA can be detected by flow cytometry.
D000230:Adenocarcinoma; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D003110:Colonic Neoplasms; D004247:DNA / Q000096:biosynthesis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007399:Interphase; D008938:Mitosis; D011419:Propidium
D011419:Propidium; D004247:DNA; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole