A new lymphocyte surface protein present in normal urine. I. Isolation and physicochemical properties.
A lymphocyte surface glycoprotein designated urinary acidic antigen (UA) has been isolated from normal urine by a combination of preparative isoelectric focusing and ammonium sulfate precipitation. It has an m.w. of 14,000 to 17,500 daltons, and is approximately 60% carbohydrate and 40% amino acid in content. The protein exhibited the following physical properties: S20,omega = 1.24; v = 0.693 ml/g; E1%1 cm, 278 nm = 2.08; and pI-2.5. It appears to be unrelated to beta 2-microglobulin, protein HC, urinary proteose, microglobulin, or any previously described normal urine or human serum protein.
D000596:Amino Acids; D000954:Antigens, Surface / Q000302:isolation & purification* / Q000652:urine; D002241:Carbohydrates; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D007525:Isoelectric Focusing; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008970:Molecular Weight
D000596:Amino Acids; D000954:Antigens, Surface; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins