T lymphocytes in malignant, non-lymphoid human tumours detected by esterase technique.
The alpha-naphthyl acetate esterase (ANAE) method, leading to distinct brownish-red dots in the cytoplasm of T lymphocytes, was used to identify these cells in cryostat sections from eighteen malignant tumours. Esterase-positive lymphocytes were found in all tumours, the highest number in the peritumoural stroma. The reliability of the method was tested on lymph nodes, on fixed E-rosetting lymphocytes and on cryostat sections from peripheral blood lymphocytes.
D002265:Carboxylic Ester Hydrolases; D004796:Clinical Enzyme Tests; D006651:Histocytochemistry; D006801:Humans; D009283:Naphthol AS D Esterase; D009369:Neoplasms / Q000201:enzymology / Q000276:immunology*; D012397:Rosette Formation; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology*
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases