Immunocytochemical demonstration of alpha-fetoprotein uptake by primary cultures of fetal hemisphere cells from mouse brain.
Mouse alpha-fetoprotein (AFP) was added for 24 h to primary cultures of mechanically dissociated mouse brain fetal hemisphere cells grown in a serum-free medium. The intracellular presence of AFP could be demonstrated by immunocytochemical methods mainly in the cytoplasm of numerous differentiating neurons. The staining occurred only after 6 days in culture. The whole network of neurites, straight processes and filaments also appeared positively labeled. No AFP was noticeable in control cultures grown in the absence of the protein.
D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D005333:Fetus; D007124:Immunoenzyme Techniques; D051379:Mice; D009474:Neurons / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism*
D000509:alpha-Fetoproteins