Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli.
A gene sequence for the fowl plague virus (FPV) haemagglutinin molecule has been inserted into a bacterial plasmid such that its transcription is under the control of a promoter derived from the tryptophan operon. Such plasmids direct the synthesis of a protein that reacts specifically with antisera to FPV haemagglutinin. Evidence is also presented that in some cases DNA inserted at the HindIII site of pBR322 is expressed.
D000956:Antigens, Viral / Q000235:genetics*; D004274:DNA, Recombinant; D000939:Epitopes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006389:Hemagglutinins, Viral / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D009876:Operon; D010957:Plasmids; D014158:Transcription, Genetic; D014364:Tryptophan / Q000235:genetics
D000956:Antigens, Viral; D004274:DNA, Recombinant; D000939:Epitopes; D006389:Hemagglutinins, Viral; D014364:Tryptophan