A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol.
We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV ws 4.1% (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0% (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9% at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically correlated well with its calculated concentrations obtained by the precipitation method. Measurement of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.
D001774:Blood Chemical Analysis / Q000379:methods*; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood*; D003911:Dextrans; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D011786:Quality Control
D003911:Dextrans; D008075:Lipoproteins, HDL; D002784:Cholesterol