title: An approach to modification of the repair activity in eucaryotic cells by biological factors.
abstract: This work was devoted to investigation or repair regulation by biological factors: viruses and interferon. DNA damage induced by gamma- and UV-irradiation, ethyleneimine and 4-nitro-quinoline-1-oxide (4-NQO) were studied, by sedimentation of lysed cells through alkaline sucrose gradients, by hydroxylapatite column chromatography and by the chromosomal aberration test. The reproducible vaccinia virus resulted in simulation repair activity of chick embryo cells after treatment with 4-NQO. Interferon, added after gamma- and UV-irradiation, decreased the chromosomal aberration level, stabilized it after ethyleneimine treatment and also stimulated the ability of cells to rejoin DNA breaks induced by 4-NQO. The cause of this phenomenon is discussed.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D002869:Chromosome Aberrations / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects; D004622:Embryo, Mammalian; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D051381:Rats; D014616:Vaccinia virus
chemical_list: D007372:Interferons