The use of frozen-thawed lymphocytes for DRw typing.
Results of DRw typing using frozen-thawed plastic "adhered" cell preparations were comparable to those obtained using fresh sheep E-rosetted lymphocytes. Estimates of B-cell content of such preparations may be erroneously low if surface immunoglobulin fluorescence is the only method used.
D001402:B-Lymphocytes / Q000276:immunology; D001788:Blood Grouping and Crossmatching; D002448:Cell Adhesion; D002469:Cell Separation; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D005615:Freezing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation
D000939:Epitopes; D011947:Receptors, Antigen, B-Cell