A rapid method for isolation of antigenically active human cell surface antigens associated with beta 2-microglobulin using a monoclonal antibody.
Serologically and biochemically pure HLA antigens and other beta 2-microglobulin-associated proteins were isolated using a rapid immunoprecipitation technique with a monoclonal anti-beta 2-microglobulin reagent A88. Elution of the immunoprecipitate with beta 2-microglobulin isolated from kidney patients proved to be efficient. Analysis of the eluate showed that the antigen preparation does not contain any immunoglobulins, retains antigenic activity and can be used for protein micelle formation. This method of preparation of human beta 2-microglobulin-associated membrane antigens has several advantages over classical immunoadsorbent techniques.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology; D001609:Beta-Globulins / Q000302:isolation & purification*; D006680:HLA Antigens / Q000302:isolation & purification*; D006801:Humans; D007163:Immunosorbent Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D013997:Time Factors; D001613:beta 2-Microglobulin / Q000302:isolation & purification*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001609:Beta-Globulins; D006680:HLA Antigens; D001613:beta 2-Microglobulin