Block of glioma cell line in S by interferon.
The human glioma cell line U-251 MG, with a well-characterized defect in growth control, was sensitive to the antiproliferative effects of human (fibroblast) interferon (IFN). IFN inhibited exponentially growing cells by increasing the number of cells in the S stage of the cell cycle. At the same time the number of cells in Go/G1 diminished. The rate of thymidine incorporation was decreased during the first cell cycle, with no prolongation of S. However, in synchronized cultures, the wave of cells with a S-phase content did not decrease over a time period several hours longer than the length of S measured by pulse labelling. Thus we conclude that, at a sufficient dose, the cells were unable to accomplish cell division as they prematurely stopped synthesizing DNA.
D000970:Antineoplastic Agents / Q000627:therapeutic use; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005470:Fluorometry; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D007372:Interferons / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D007372:Interferons