Growth inhibition of Staphylococcus aureus after experimental infection of the udder by high and low concentration of lactoferrin and lysozyme in milk.
Ten Holstein-Friesian cows were distributed according to their lactoferrin and lysozyme concentrations in milk into groups with high and low concentrations. In each cow, a front and a rear mammary quarter was infected by inoculation of 10(8) colony forming units of Staphylococcus aureus while the other two quarters were infused with 2 ml of sterile milk. The reaction was observed during the following nine days. After 10 hours the cell count, the lactoferrin and lysozyme concentrations were increased in the infected and control quarters. In milk samples with a high initial lactoferrin concentration the colony forming units of S. aureus were higher than in those with a low concentration. In milk samples with a high lysozyme concentration with colony forming units of S. Aureus were significantly lower than in those with low concentrations. These results show, that the lysozyme concentration in milk of healthy udders could indicate the preparedness for defense against infectious diseases.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005260:Female; D007781:Lactoferrin / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis*; D008321:Mammary Glands, Animal / Q000382:microbiology; D008892:Milk / Q000032:analysis* / Q000201:enzymology / Q000382:microbiology; D009113:Muramidase / Q000032:analysis*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000254:growth & development
D007782:Lactoglobulins; D009113:Muramidase; D007781:Lactoferrin