Stabilization of transcobalamins against heating on sephadex G-50.
The human serum stabilized on Sephadex G-50 did not show any changes in the distribution of radioactive vitamin B12 bound to transcobalamins during incubation at 56 degrees C (30 and 120 min.) and 80 degrees C (20 min.), whereas, in the non-stabilized serum, heating increased the vitamin 57CoB12 in transcobalamin I and III fraction and decreased or completely abolished the amount of radioactive vitamin B12 bound to transcobalamin II.
D001798:Blood Proteins; D003911:Dextrans; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D011485:Protein Binding; D014155:Transcobalamins / Q000097:blood*; D014805:Vitamin B 12 / Q000378:metabolism
D001798:Blood Proteins; D003911:Dextrans; D014155:Transcobalamins; C025614:sephadex; D014805:Vitamin B 12