Human fibroblast interferon. An improved purification.
Human fibroblast interferon has been purified 2,900-fold to homogeneity. The purification is achieved in two steps by chromatography on blue Sepharose. The specific activity of the homogeneous interferon is 5 X 10(8) units/mg and the yield of biological activity has ranged from 20-40%. The interferon can exist as a monomer (Mr = 20,000) and as a dimer (Mr - 40,000). The dimer can be converted to the monomer by heating in sodium dodecyl sulfate and thioglycolic acid.
D002460:Cell Line; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D007372:Interferons / Q000302:isolation & purification*; D008970:Molecular Weight; D012685:Sepharose / Q000031:analogs & derivatives
C029483:Blue-Sepharose; D007372:Interferons; D012685:Sepharose