Comparative permeability of different glioma models to horseradish peroxidase.
Seven different experimental glioma models were studied to determine their capillary permeability to horseradish peroxidase. The models were the autochthonous ASV-viral model, intracerebral (ic) and (sc) injections of rat 9L and RG-2 tumor cell lines, the sc rat S-69C15 tumor cell line, and human glioblastoma cell lines in nude mice. The ASV-viral model showed an average HRP permeability of 63% of tumor volume, the ic RG-2 tumors were 100% permeable, and the ic 9L tumors were 41% permeable. The permeability of the marginal zone (brain-tumor interface) showed similar variation from group to group. In contrast, all of the sc tumors were 100% permeable regardless of the cell line used to create the tumors. Our results show the variability between these glioma models, and suggest that RG-2 ic gliomas and all sc gliomas should be optimal to assess the tumoricidal effect of drugs, because access to the tumor compartment from the vascular compartment is complete.
D000818:Animals; D001370:Axonal Transport; D001932:Brain Neoplasms / Q000473:pathology / Q000503:physiopathology*; D002460:Cell Line; D005910:Glioma / Q000473:pathology / Q000503:physiopathology*; D006735:Horseradish Peroxidase; D006801:Humans; D009374:Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology; D010539:Permeability; D051381:Rats
D006735:Horseradish Peroxidase