Expression of a human gene for interferon in yeast.
A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5'-flanking sequences of the Saccharomyces cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli. Yeast cells transformed by these plasmids synthesize up to 1 x 10(6) molecules of biologically active LeIF-D per cell.
D000429:Alcohol Oxidoreductases / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D007372:Interferons / Q000235:genetics*; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D007372:Interferons; D000429:Alcohol Oxidoreductases