A convenient replacement for cyanogen bromide-activated solid phases in immunoradiometric assays.
Periodate oxidation of Sephacryl and Sephadex gels (Pharmacia) and the subsequent coupling of the IgG fraction from antisera has been investigated as a possible, more convenient system to replace the potentially hazardous cyanogen bromide activation of Sepharose which is currently used to produce solid-phase linked antibody for use in sandwich immunoradiometric assays (IRMA). A solid phase based on Sephacryl S300 was found to be a suitable replacement. Under optimum conditions of periodate oxidation and protein concentration during coupling a Sephacryl S300 solid-phase antibody was produced which, in an IRMA for human alphafetoprotein, behaved favourably in terms of high binding capacity and low non-specific binding. Both the final solid-phase preparation and the intermediate oxidised gel were stable for several weeks.
D000180:Acrylic Resins / Q000494:pharmacology; D000818:Animals; D003488:Cyanogen Bromide / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010504:Periodic Acid / Q000378:metabolism; D011137:Polystyrenes / Q000494:pharmacology; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000276:immunology
D000180:Acrylic Resins; D003911:Dextrans; D007074:Immunoglobulin G; D011137:Polystyrenes; C037239:Sephacryl Superfine; D000509:alpha-Fetoproteins; D010504:Periodic Acid; C023049:sephacryl S 200; C025614:sephadex; D003488:Cyanogen Bromide