Isolation of a serum component that stimulates the spreading of cells in culture.
A foetal-calf-serum component has been isolated that stimulates cells in culture to adopt typical fibroblast morphology. This "spreading" factor is a glycoprotein with a mol. wt. of approx. 62000 and contains 12% carbohydrate; it has an isoelectric point of 4.8. The method described allows purification to homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and analytical isoelectric focusing. Under cell-culture conditions the factor is active at 18 micrograms/ml.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media / Q000032:analysis; D003956:Dialysis; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D005312:Fetal Blood / Q000032:analysis*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006023:Glycoproteins / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology; D007525:Isoelectric Focusing; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D019096:Vitronectin
D000596:Amino Acids; D003470:Culture Media; D006023:Glycoproteins; D019096:Vitronectin