Amplified ribosomal RNA genes in a rat hepatoma cell line are enriched in 5-methylcytosine.
In a rat hepatoma cell line, H4-IIE-C3, a 10-fold excess of 18S and 28S rRNA genes has been found in amplified chromosome regions. Antibodies to 5-methylcytidine bound extensively to the DNA of these regions, indicating a high level of DNA methylation. Most of the amplified rRNA genes were transcriptionally inactive, as shown by their failure to stain with silver. DNAs from the tumor cells and control rat hepatocytes grown with L-[methyl-14C]methionine were digested with restriction endonuclease EcoRI; the DNA fragments were separated by agarose gel electrophoresis, denatured, transferred to nitrocellulose filters, and hybridized to 32P-labeled rRNA or cDNA. Fragments containing the 18S or 28S rRNA coding sequences occurred in three major size classes; all three were rich in 5-methylcytosine. Analysis of EcoRI fragments of DNA from the tumor and control cells after digestion with Hpa II or Msp I endonuclease indicated that the 5'-C-C-G-G-3' sequences in most of the amplified rRNA genes were methylated. Analysis of the fragments produced by digestion with Hha I endonuclease indicated a high degree of methylation within its recognition sequence in the amplified rRNA genes as well. The association of hypermethylation with restricted transcriptional activity suggests that DNA methylation may regulate the activity of the rRNA genes.
D044503:5-Methylcytosine; D000818:Animals; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D005786:Gene Expression Regulation; D005796:Genes; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012835:Silver Nitrate; D013194:Staining and Labeling; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D044503:5-Methylcytosine; D003596:Cytosine; D012835:Silver Nitrate; D004262:DNA Restriction Enzymes