Visualization of isozymes which generate inorganic phosphate.
A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D004285:Dogs; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D010637:Phenolsulfonphthalein; D010710:Phosphates; D010920:Placenta / Q000201:enzymology; D013194:Staining and Labeling
D007527:Isoenzymes; D010710:Phosphates; D000469:Alkaline Phosphatase; D010637:Phenolsulfonphthalein