title: Effects of insulin on ribonucleic acid synthesis in toad urinary bladder.
abstract: We previously found that the sustained rise in active sodium transport by the toad urinary bladder after exposure to insulin is associated with the synthesis of a membrane protein with a molecular weight of 25,000. Studying the effects of insulin on the incorporation of uridine into RNA of epithelial cells of the bladder, we now report that insulin causes a 39% increase in the incorporation of [3H]uridine into poly(A)-containing RNA extracted from granular cells, the predominant epithelial cell type. The increased accumulation of label in RNA, most notably species ranging from 5-10S, could be demonstrated whether cells were pulsed with [3H]uridine or continuously exposed to the label. Using a reticulocyte lysate system, we compared the translation products of RNA from untreated and insulin-treated granular mucosal cells. Exposure to insulin was associated with the expression of a mRNA coding for a protein with molecular weight of approximately 25,000, similar in size to the insulin-induced membrane protein we previously identified in the intact tissue.
mesh_terms: D000818:Animals; D002024:Bufo marinus; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D009092:Mucous Membrane / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D014158:Transcription, Genetic / Q000187:drug effects*; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA