Determination of DNA surplus in the nuclei of some cerebellar Purkinje cells with different cytochemical methods.
Cytofluorometry and cytophotometry (Feulgen staining) as well as ultraviolet cytophotometry revealed similar histograms of DNA content in squashed nuclei of rat cerebellar Purkinje cells. More than 90% diploid (2c) cells and 1% tetraploid (4c) cells were obtained. DNA content in other Purkinje cells ranged between the 2c and 4c level.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005470:Fluorometry; D011689:Purkinje Cells / Q000032:analysis*; D051381:Rats; D012394:Rosaniline Dyes; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
D012394:Rosaniline Dyes; D004247:DNA