Metabolism of 5-fluorouracil in various human normal and tumor tissues.
The metabolism of 5-fluorouracil (5-FU) in tumors and normal tissues of humans was investigated in vitro. Phosphorylation of 5-FU was faster in tumor tissues than in normal tissues. Phosphorylating activity with 2-deoxy-alpha-D-ribose 1-phosphate (dRiblP) and ATP as cofactors was more active than that with alpha D-ribose 1-phosphate (RiblP) and ATP, or 5-phospho-alpha-D-ribosyldiphosphate (PPRibP) as cofactors. Phosphorylating activity in squamous cell carcinoma of the lung was similar to that in adenocarcinomas. Degradation of 5-FU was much faster in the liver than in other tissues including tumor tissues.
D000230:Adenocarcinoma / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D005472:Fluorouracil / Q000378:metabolism*; D005770:Gastrointestinal Neoplasms / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D010754:Phosphoribosyl Pyrophosphate / Q000494:pharmacology; D010766:Phosphorylation; D012267:Ribosemonophosphates / Q000494:pharmacology
D012267:Ribosemonophosphates; C031154:ribose 1-phosphate; C031302:2-deoxyribose 1-phosphate; D010754:Phosphoribosyl Pyrophosphate; D000255:Adenosine Triphosphate; D005472:Fluorouracil