Interactions of basic polypeptides and proteins with calmodulin.
Low concentrations (less than 10 microgram/ml) of a number of highly basic polypeptides inhibit the calmodulin-stimulated cyclic nucleotide phosphodiesterase. Inhibitory compounds include synthetic polypeptides [polylysine (D and L) and polyarginine] and basic proteins (protamine, histones H1, H2A, H2B, H3 and H4 and myelin basic protein). Polylysine of mol.wt. about 2000 or higher was inhibitory, but pentalysine did not inhibit. Other basic proteins and compounds did not inhibit, including bradykinin, spermine and putrescine. In mixtures of calmodulin and basic protein, complexes were formed whether Ca2+ was present or not. This was true for polylysine, myelin basic protein and histone H2B. These interactions suggest that the inhibition of the phosphodiesterase is due to interaction of these basic proteins with calmodulin. The wide variety of basic polypeptides and proteins that affect the calmodulin stimulation of phosphodiesterase indicates that these interactions are not specific.
D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D006657:Histones / Q000494:pharmacology; D004676:Myelin Basic Protein / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors; D011107:Polylysine / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D006657:Histones; D004676:Myelin Basic Protein; D010455:Peptides; D010726:Phosphodiesterase Inhibitors; D011479:Protamines; D011506:Proteins; D011107:Polylysine