A light microscopic study of the development and behaviour of the synaptonemal complex in spermatocytes of the mouse.
A method is presented for the sequential analysis of male meiosis using hydroxyurea (HU). HU produces a gap in the spermatogenic line. The front of surviving cells behind the gap was examined day by day using silverstained whole mount spreads on glass slides. With this method it was possible to study the development and behaviour of the synaptonemal complex (SC) in mouse spermatocytes by the light microscope. At zygotene no unpaired axial elements could be seen. Unpaired axial elements were found to be specific for the diplotene stage. The axes of the XY pair could be recognized from late zygotene up to diplotene.
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D003584:Cytological Techniques; D006918:Hydroxyurea; D008297:Male; D008540:Meiosis; D051379:Mice; D012834:Silver; D013090:Spermatocytes / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*; D013194:Staining and Labeling
D012834:Silver; D006918:Hydroxyurea