Monoclonal antibody against an Ir gene product?
Genetic, biochemical, and functional studies have been performed using a monoclonal antibody, Y-17, directed at a conformational or combinatorial determinant formed by certain Ae:E alpha complexes. This determinant appears to be a marker present on a subset of B cells as well as on non-T and non-B spleen cells. Besides Ae and E alpha chains, Y-17 precipitates a third chain that is indistinguishable from the A alpha chain in two-dimensional gels. This results suggests additional combinatorial complexity in the generation of I-region encoded antigens. Y-17 can inhibit the response of T cells to Ae:E alpha determinants in mixed lymphocyte cultures. Furthermore, Y-17 blocks antigen-specific T cell proliferative responses to GLPhe and pigeon cytochrome c which have been shown to require the Ae:E alpha complex as a restriction element for antigen presentation. These results provide strong evidence for the molecular identity of Ia antigens, Ir-gene products and Lad antigens.
D000818:Animals; D000939:Epitopes / Q000235:genetics; D005802:Genes, MHC Class II; D005819:Genetic Markers; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D005819:Genetic Markers; D007130:Immunoglobulin Idiotypes