Tissue culture of normal human post-pubertal prostate.
Total prostatectomies were performed in 10 young male cadaver renal donors and explant cultures were prepared in Falcon flasks. Growth of a monolayer of predominantly epithelial cells occurred rapidly (latent time 3-5 days) and the flasks became confluent within 15-17 days. The monolayers grew well even when a needle was used to circumscribe and remove the explants. Passaging of monolayer cells by seeding at a density of 5 x 10(5) resulted in the vigorous growth of epithelial cells. However, the cultures were eventually overgrown by fibroblasts. Similar explant cultures of benign prostatic hyperplasia or prostatic carcinoma demonstrated a longer latent time, rarely attained confluence, died after explant removal, and could not be passaged.
D000230:Adenocarcinoma / Q000473:pathology; D000293:Adolescent; D000328:Adult; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology*; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology