Isolation of plasma membranes from ram spermatozoa by a two-phase polymer system.
The plasma membranes of ram spermatozoa were disrupted in a hypotonic EDTA medium and isolated by using a two-phase polymer system of dextran--polyethyleneglycol. The plasma membranes obtained were of a relatively high degree of purity (approximately 70%) as judged by electron microscopy observations and measurements of the marker enzymes alkaline phosphatase, ATPase and AMPase. The activity of succinate cytochrome C reductase, a marker of mitochondrial membranes, was very low.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000648:ultrastructure; D003911:Dextrans; D008297:Male; D011092:Polyethylene Glycols; D012756:Sheep; D013094:Spermatozoa / Q000648:ultrastructure*
D003911:Dextrans; D011092:Polyethylene Glycols