Stimulation of prostaglandin E production by bacterial endotoxins in cultured human synovial fibroblasts.
E coli, shigella, salmonella, and cholera endotoxins stimulated prostaglandin E (PGE) production by cultured human synovial and foreskin fibroblasts. The minimal effective dose of Shigella endotoxin was 2 micrograms/ml and a maximal response was observed at 10 micrograms/ml. PGE stimulation was first detected 7 hours after addition of cholera endotoxin. Stimulation by shigella endotoxin of both PGE and hyaluronic acid production was inhibited by indomethacin and aspirin. The present results suggest that PGE is a mediator of joint inflammation induced by endotoxins.
D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D005347:Fibroblasts; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007372:Interferons / Q000096:biosynthesis; D011070:Poly I-C / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D012485:Salmonella typhi; D012763:Shigella flexneri
D004731:Endotoxins; D011458:Prostaglandins E; D006820:Hyaluronic Acid; D007372:Interferons; D011070:Poly I-C