[Obtaining a productive strain of Streptoverticillium griseocarneum var. bleomycini that yields the new antineoplastic antibiotic, bleomycetin].
Selection of the organism producing bleomycin, a multicomponent antitumor antibiotic, was performed. The aim was the selection of a more active strain with preferable synthesis of component A5 (bleomycetin) of the bleomycin complex. Optimal variants of the conditions for mutagensis with UV light and gamma-rays in step-wise selection of the active strain were determined and a possibility of selecting analog-resistant mutants with the use of structural analogs of the metabolites participating in synthesis of the bleomycin molecule was studied. A mutant analog-resistant strain capable of supersynthesis of bleomycin A5 (bleomycetin) was selected, the biosynthetic capacity of Streptoverticillum griseocarneum var. bleomycini being increased at least 19 times.
D001761:Bleomycin / Q000096:biosynthesis*; D005720:Gamma Rays; D014644:Genetic Variation / Q000528:radiation effects; D009154:Mutation; D012641:Selection, Genetic; D012988:Soil Microbiology; D013306:Streptomycetaceae / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D001761:Bleomycin
Poluchenie produktivnogo shtamma Streptoverticillium griseocarneum var. bleomycini, obrazuiushchego novyĭ protivoopukholevyĭ antibiotik bleomitsetin.