In vitro transfer of reactivity to Trypanosoma cruzi antigens from rat cells to human cells with immune RNA.
The immunologic activity of polysomal RNA isolated from the spleens of rats infected with Trypanosoma cruzi was assessed by the leukocyte migration-inhibition assay as an in vitro correlate of delayed hypersensitivity. This RNA preparation transferred reactivity to T. cruzi antigens to nonsensitized human lymphocytes from peripheral blood. This transfer of reactivity was abolished by treatment of the RNA preparation with ribonuclease. The transfer of delayed hypersensitivity in vitro from rat cells to human cells was antigen-specific. Polysomal RNA also conferred significant protection against infection with T. cruzi in mice, as evaluated by the level of parasitemia and the survival rate of mice challenged with virulent strain of Y of T. cruzi.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D014355:Chagas Disease / Q000276:immunology*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007112:Immunity, Maternally-Acquired / Q000187:drug effects; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D012313:RNA / Q000276:immunology*; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology
D000941:Antigens; D012313:RNA; D012260:Ribonucleases