Effect of unilateral nephrectomy on the RNA synthesis in hypertrophic rat kidney.
1. The hypertrophic rat kidney after unilateral nephrectomy showed a time-dependent increase in the RNA/DNA ratio. This was associated with the increased rate of alpha-amanitin-insensitive RNA synthesis in isolated nuclei. 2. The increased activity of solubilized RNA polymerase was due to polymerase I, II and III as judged from the DEAE-Sephadex chromatography. 3. Chromatin DNA from hypertrophic kidneys was more susceptible to DNAase I than DNA from control kidneys.
D000818:Animals; D002843:Chromatin / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D006984:Hypertrophy / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007700:Kinetics; D008297:Male; D009392:Nephrectomy; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
D002843:Chromatin; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I