title: A standardized differential stain for hematology.
abstract: A stain composed of purified Azure B and Eosin Y has been developed for use in conjunction with an automated stainer. This system provides the consistency in chromatic properties required for automated blood-cell analysis by pattern recognition systems. The stain represents a marked improvement in stability over those presently used with differential analyzers, but it is also an important advance toward a standardized stain for general hematological use. The purity of the component dyes, the stability of the final stain and the dependence of staining intensity on the stain/buffer ratio and dye concentration are discussed.
mesh_terms: D001331:Automation; D001399:Azure Stains; D004801:Eosine Yellowish-(YS); D006405:Hematology / Q000379:methods*; D010640:Phenothiazines; D013194:Staining and Labeling
chemical_list: D001399:Azure Stains; D010640:Phenothiazines; D004801:Eosine Yellowish-(YS)