title: Determination of DNA surplus in the nuclei of some cerebellar Purkinje cells with different cytochemical methods.
abstract: Cytofluorometry and cytophotometry (Feulgen staining) as well as ultraviolet cytophotometry revealed similar histograms of DNA content in squashed nuclei of rat cerebellar Purkinje cells. More than 90% diploid (2c) cells and 1% tetraploid (4c) cells were obtained. DNA content in other Purkinje cells ranged between the 2c and 4c level.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005470:Fluorometry; D011689:Purkinje Cells / Q000032:analysis*; D051381:Rats; D012394:Rosaniline Dyes; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
chemical_list: D012394:Rosaniline Dyes; D004247:DNA