The immunohistochemical detection of prostatic acid phosphatase: its possibilities and limitations in tumour histochemistry.
The immunological specificity of the Amsterdam rabbit antiserum against human prostatic acid phosphatase was studied on paraffin sections of 200 prostatic carcinomas and 330 control tissues using an indirect peroxidase technique. Peripheral blood leucocyte smears were also investigated with a fluorescent technique. In a limited number of cases, the mixed aggregation immunocytochemical method was also applied as post-primary incubation procedure. The diaminobenzidine (DAB) final reaction product of the peroxidase technique, carried out under standard conditions, was quantified in some cases using the Leyden Television Analysis System (LEYTAS) with a built-in standard. A positive reaction was obtained in 96.5%  of the prostatic carcinomas. Only 2.1% of the non-prostatic tumour cases (23 types) showed a positive reaction, namely six out of 10 insulomas and one out of 10 carcinoid tumours. The beta-cells of the normal islet of Langerhans and the leucocytes in the smears showed a positive reaction. The sensitivity of the peroxidase method, judged subjectively, is not only dependent on the circumstances of fixation, embedding and incubation but also on the degree of tumour differentiation. None of the three prostatic carcinomas studied reached the level of DAB staining intensity shown by the hyperplastic prostatic epithelium.
D000135:Acid Phosphatase / Q000378:metabolism*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000201:enzymology* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology; D013194:Staining and Labeling; D014018:Tissue Distribution
D007202:Indicators and Reagents; D007527:Isoenzymes; D000135:Acid Phosphatase