Preservation of red blood cells with purines and nucleosides. I. Changes of ATP, 2,3-P2G and further parameters of metabolism in red cells stored as whole blood and as resuspension at 4 degrees C and 25 degrees C.
CPD blood with addition of adenine in 0.25 mM final concentration alone or in combination with guanosine and inosine each in 0.5 mM concentration and also with a higher adenine concentration--0.75 mM--was stored 6 weeks at 4 degrees C. In a second experiment red cell concentrates were prepared from CPD blood and resuspended in the same volume of a glucose-sucrose-citrate solution with the addition of adenine or guanine and both in combination with inosine. The resuspended cells were stored 7 days at 25 degrees C. Adenine and guanosine added simultaneously were most effective on the maintenance of ATP and P2G content. When guanosine was replaced by the same amount of inosine, no effect on P2G content was found in comparison with the addition of only adenine, however, the ATP content decreased more rapidly. The reason for this difference can be due to a different affinity of the IMP/GMP: pyrophosphate phosphoribosyltransferase to their substrates as well as regulation principles in the P2G bypass. The guanosine effect on red cell preservation consists in improved viability of red cells in vivo, increased nucleotide synthesis, increased formation of 2.3-P2G, delayed disc-spher transformation of red cells and decreased hemolysis. The most effective solution for red cell preservation should contain adenine, a pentose moiety like guanosine, and inorganic phosphate in a final concentrations in blood of 0.25 : 0.5 : 2 mM. The preparation of red cell concentrates from CPD-AG or CPD-A blood requires an elevation of glucose concentration in blood above 30 mM for storage periods up to 5--6 weeks.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009705:Nucleosides / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology
D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D009705:Nucleosides; D011687:Purines; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate