Inhibition of reverse transcriptase by polyvinyl sulfate (PVS).
The effect of the ribonuclease inhibitor polyvinyl sulfate on the activity of retroviral DNA polymerase and terminal c transferase was examined. This substance was found to be a potent inhibitor of these enzymes by virtue of competition between the sulfated sidechains of the molecule and the template primer for a site on the enzyme. The significance of these findings in relation to searching for reverse transcriptase is discussed.
D004254:DNA Nucleotidyltransferases / Q000037:antagonists & inhibitors; D007700:Kinetics; D008979:Moloney murine leukemia virus / Q000201:enzymology; D019384:Nucleic Acid Synthesis Inhibitors; D011145:Polyvinyls / Q000494:pharmacology*; D011925:Rauscher Virus / Q000201:enzymology; D012190:Retroviridae / Q000201:enzymology*; D018894:Reverse Transcriptase Inhibitors; D012842:Sarcoma Virus, Woolly Monkey / Q000201:enzymology; D013045:Species Specificity
D019384:Nucleic Acid Synthesis Inhibitors; D011145:Polyvinyls; D018894:Reverse Transcriptase Inhibitors; C019050:polyvinyl sulfate; D004254:DNA Nucleotidyltransferases