title: Influence of two different fixatives on the identification of plasma cells in human rectal mucosa.
abstract: Plasma cells in sections of bisected human rectal biopsy specimens, fixed in two alternative fixatives, were enumerated after staining by an indirect immunoperoxidase procedure intended to demonstrate immunoglobulin-containing cells. The counts of immunoperoxidase-positive plasma cells were significantly higher after fixation in formol sublimate than after fixation in formol saline. Formol sublimate appears to be a more reliable fixative than formol saline for specimens of rectal mucosa in which quantitation of plasma cells, stained for intracellular immunoglobulin by an immunoperoxidase technique, is intended.
mesh_terms: D001706:Biopsy; D003109:Colonic Diseases, Functional / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis; D007413:Intestinal Mucosa / Q000473:pathology*; D012007:Rectum / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D007136:Immunoglobulins