title: A modification of the adenosine triphosphatase method to demonstrate epidermal Langerhans cells.
abstract: Epidermal Langerhans cells are most commonly demonstrated by utilizing their adenosine triphosphatase reactivity. Although this is one of the best methods available to the light microscopist, it is a capricious technique which does not always permit optimal demonstration of this cell population. Prolonged fixation in cacodylate formalin and incubation in 1.32 X 10-3 molar adenosine triphosphatase permits reliable reproduction and good definition of these dendritic cells.
mesh_terms: D000251:Adenosine Triphosphatases; D000818:Animals; D000078404:Epidermal Cells; D005404:Fixatives; D006168:Guinea Pigs; D006801:Humans; D007801:Langerhans Cells / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005404:Fixatives; D000251:Adenosine Triphosphatases