Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product.
We previously determined the nucleotide sequence of the 3' end of Moloney leukaemia virus and discovered the potential coding region for an unknown protein, R. We now show that this region does encode a protein. A pentadecapeptide of R was chemically synthesized and antibodies raised against it. Antisera to the synthetic peptide recognize the R protein and the env precursor polyprotein in infected cells. The strategy presented here should provide a general method for accessing proteins predicted by nucleotide sequences.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes; D005814:Genes, Viral; D007158:Immunologic Techniques; D008979:Moloney murine leukemia virus / Q000235:genetics*; D014764:Viral Proteins / Q000138:chemical synthesis / Q000235:genetics*
D004279:DNA, Viral; D000939:Epitopes; D014764:Viral Proteins