The stimulus-induced release of unmetabolized 5-hydroxytryptamine from superfused rat brain synaptosomes.
A number of existing techniques have been modified to produce an improved technique for examining the depolarization-induced release of unmetabolized 5-hydroxytryptamine (5-HT) from drug-free superfused synaptosomes. Synaptosomes which have been pre-loaded with [3H]5-HT are set up in perfusion chambers and continuously perfused with medium. Serial samples of effluent perfusate are collected on ice and the released 5-HT and 5-hydroxy-indole acetic acid (5-HIAA) are separated by ion exchange chromatography. The technique provides reproducible Ca2+-dependent responses to K+ depolarization in the absence of monoamineoxidase inhibition and free from the interference of re-uptake. The advantages of the use of the system in examining transmitter release and its modulation is discussed.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid