Determination of 5-hydroxyindoleacetic acid in urine by high-performance liquid chromatography: a fully automated method.
High-performance liquid anion-exchange chromatography is combined with continuous flow spectrofluorometry at an optimized pH for the determination of 5-hydroxyindoleacetic acid (5-HIAA) in urine. Its chief advantages are speed (7 min per determination), enabling measurement of large series of samples, precision (coefficient of variation = 0.72%), sensitivity (detection limit: 1 pmol) and specificity (no interference with 5-HIAA precursors or with the drugs tested). Reference values (n = 112) are 25 +/- 25 mumol per 24 h. Comparison with a reference spectrofluorometric technique shows a correlation coefficient of 0.96.
D001331:Automation; D002276:Carcinoid Tumor / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D007414:Intestinal Neoplasms / Q000175:diagnosis; D007421:Intestine, Small
D006897:Hydroxyindoleacetic Acid