Vasoactive intestinal polypeptide-like immunoreactivity in a human neuroblastoma cell line and the coexistence of other neuropeptide immunoreactivity in the cell line.
The presence of VIP-like immunoreactivity in human neuroblastoma cell line NB-1 was demonstrated by radioimmunoassay of the crude cell extract and by immunocytochemical staining of the cells. Gel filtration profiles of VIP-like immunoreactivity in the cell extract measured by radioimmunoassays using three different region-specific antisera revealed that the immunoreactivity consists of a major molecular form corresponding to porcine VIP having 28 amino acid residues with at least two additional minor forms larger and smaller than the VIP. In addition to VIP-like immunoreactivity, the cell extract was shown to contain substance P-, neurotensin- and somatostatin-like immunoreactivity as well.
D000818:Animals; D002460:Cell Line; D005260:Female; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D006801:Humans; D009447:Neuroblastoma / Q000032:analysis*; D009496:Neurotensin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013552:Swine; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D005768:Gastrointestinal Hormones; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D013004:Somatostatin