[Evolutionary origin of the T-lymphocyte receptor. II. Production and partial characterization of antisera to the immunoglobulin-like cell membrane protein from thymocytes of the carp].
An immunoglobulin-like protein from Triton X-100 solubilized cell surface proteins from thymocytes can be isolated by specific precipitation using anti-IgM antibodies. Immunizing rabbits with those immune complexes we succeeded to elicit anti-thymocyte mIg sera reacting with both thymocyte mIg and IgM. SDS-PAGE analysis revealed that anti-IgM and anti-thymocyte mIg sera recognize the same thymocytic surface protein. The cross-reactivity between thymocyte mIg and IgM is caused by protein determinants very similar to each other. Anti-thymocyte mIg sera absorbed with IgM lose their capacity to bind thymocyte mIg. However, following absorption with thymocytes anti-IgM sera still possess amounts of antibodies reacting with humoral IgM.
D000818:Animals; D000937:Antigen-Antibody Reactions; D005075:Biological Evolution; D002347:Carps / Q000276:immunology*; D003429:Cross Reactions; D003530:Cyprinidae / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007106:Immune Sera; D007075:Immunoglobulin M / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000939:Epitopes; D007106:Immune Sera; D007075:Immunoglobulin M; D008565:Membrane Proteins; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell
Evolutionärer Ursprung des T-Lymphozyten-Rezeptors. II. Herstellung und partielle Charakterisierung von Antiseren gegen das Immunglobulin-ähnliche Zellmembranprotein von Thymozyten des Karpfens.