N, N' (p-Xylylidene)-bis-aminoguanidine (2HCI) resistant mutants in Saccharomyces cerevisiae and their relation to the amino acid transport system.
N,N'-(p-Xylylidene)-bis-aminoguanidine 2HCl proved to be an efficient inducer of non-chromosomal petite mutation in Saccharomyces cerevisiae in either growing or non-growing conditions. A mutant Agr-7 resistant to this compound was obtained. Resistance proved to dominate over its XBAG-sensitive allele in the diploid formed in cross. Mutant Agr-7 is characterized by slow growth rate on YEPG or minimal medium and small colony size. Studies on uptake of amino acids and sugars indicate that the phenomenon of resistance is involved in alteration of the general amino acid permease activity.
D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport; D055598:Chemical Phenomena; D002621:Chemistry; D046508:Culture Techniques; D004352:Drug Resistance, Microbial; D006146:Guanidines / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013013:Sorbose / Q000378:metabolism; D014992:Xylenes / Q000494:pharmacology
D000596:Amino Acids; D006146:Guanidines; D026901:Membrane Transport Proteins; D014992:Xylenes; C009693:N,N-(4-xylylidene)bisaminoguanidine; D013013:Sorbose