[Isolation and characterization of the matrix properties of electrophoretically homogeneous immunoglobulin L-chain mRNA from mouse myeloma MORC 21].
mRNA with m. w. 4.10(5) dalton was isolated by elution of one of the fractions of poly(A)-RNA from polyacrylamide gel. During reelectrophoresis this mRNA was electrophoretically homogeneous and had the same size. The isolated fraction represented the template activity in a wheat germ cell-free system of protein synthesis and stimulated the synthesis of polypeptides, electrophoretically correspondent to L-chain of authentic immunoglobulin. Comparative data of properties of electrophoretically homogenous mRNA and analogous fraction of mRNA, isolated from sucrose concentration gradient after fractionation of total poly(A)-containing RNA are given.
D000818:Animals; D002474:Cell-Free System; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000235:genetics*; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000096:biosynthesis / Q000235:genetics*; D010954:Plasmacytoma / Q000032:analysis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification*; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase
D007147:Immunoglobulin Light Chains; D009194:Myeloma Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012194:RNA-Directed DNA Polymerase
Vydelenie i kharakteristika matrichnykh svoĭstv élektroforeticheski gomogennoĭ mRNK L-tsepeĭ immunoglobulina mielomy mysheĭ MORC 21.