title: Applications of synthetic oligodeoxyribonucleotides to problems in molecular biology.
abstract: The applications of synthetic oligodeoxyribonucleotides to problems in molecular biology described in this article are those where the oligodeoxyribonucleotide is a probe for a specific region of a nucleic acid. This includes the isolation of the iso-1-cytochrome c gene of yeast; the sequence determination of RNAs and DNAs including regions of double-stranded DNA; the introduction of defined site-specific point mutations into bacteriophage OX174 and in the in vitro selection of mutant DNA from a mixture with wild-type DNA.
mesh_terms: D001483:Base Sequence; D003574:Cytochrome c Group / Q000235:genetics; D004247:DNA / Q000138:chemical synthesis*; D004262:DNA Restriction Enzymes; D005796:Genes; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D012313:RNA; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D003574:Cytochrome c Group; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes