Identification and molecular characterization of macrophage-associated antigens of the rat.
Rabbit antiserum to rat peritoneal exudate (PE) macrophage (M phi) antigens was prepared and its reactivity with cell surface proteins of M phi, granulocytes, and lymphocytes was studied by one- and two-dimensional polyacrylamide gel electrophoresis (PAGE). A total of 14 membrane antigens were identified of which three were found to be expressed only by M phi and granulocytes. By one-dimensional analysis, a protein with an approximate m.w. of 105,000 was present on splenic and PE M phi and on splenic lymphocytes. Two-dimensional analysis revealed that this band was heterogeneous and contained at least three species, one of which was restricted to expression on M phi and granulocytes. A second protein of 150,000 daltons was resolved into two species by two-dimensional analysis. Both of these species were present on M phi and granulocytes but not on lymphocytes. Both the 105,000- and 150,000-dalton proteins were glycosylated. Because the 105,000- and 150-000-dalton proteins expressed by M phi were also expressed by granulocytes, is is likely that these are differentiation antigens whose expression is a characteristic property of cells within both monocytoid and myeloid lineages. All three 105,000-dalton species and one of the two 150,000-dalton species were detected on mouse M phi, indicating their expression is not unique to the rat.
D000042:Absorption; D000818:Animals; D000954:Antigens, Surface; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology; D008198:Lymph Nodes / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology*; D011920:Rats, Inbred WF / Q000276:immunology*; D013154:Spleen / Q000032:analysis
D000954:Antigens, Surface; D000939:Epitopes; D007106:Immune Sera