Immunochemical detection of vasopressin precursors: artificial processing and quantification along the hypothalamo-hypophysial axis.
A novel immunological approach to the problem of the detection and molar evaluation of vasopressin precursors was taken. First, the specificity of anti-vasopressin antibodies was studied and the hormone antigenic determinant was identified as the sequence Cys-Pro-Arg-Gly-NH2. Then this antigenic determinant, not originally shared by the precursors, was reconstituted by tryptic cleavage followed by chemical fixation of glycinamide. This treatment made quantification of precursors by radioimmunoassay possible at a fmol level in various tissues. In normal rat, precursors were found only in the supraoptic nucleus (192 pmol/mg protein), paraventricular nucleus, median eminence and posterior lobe of the hypophysis. The maturation process was followed by the decrease of the ratio of precursor to hormone from 4-5 to 0.02 along the hypothalamo-hypophysial axis. In Brattleboro rats, genetically deficient in vasopressin, no precursor could be detected over the background level; that ensures the specificity and reliability of this approach.
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000032:analysis; D007030:Hypothalamo-Hypophyseal System / Q000032:analysis*; D008297:Male; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D014667:Vasopressins / Q000032:analysis* / Q000276:immunology
D000939:Epitopes; D011498:Protein Precursors; D014667:Vasopressins