Synthesis of prematurely terminated late transcripts of polyoma virus DNA is resistant to inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.
Exposure of mouse kidney cells to 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) (75 to 300 microM) during the late phase of infection by polyoma virus resulted in nearly complete (90 to 98%) inhibition of virus RNA synthesis. Sedimentation analysis revealed that, although the synthesis of high mol. wt. (greater than 10S) virus RNA was inhibited in a manner parallel to that of total virus RNA, the synthesis of small (3S to 7S) virus RNA was inhibited by only 40 to 50% in the presence of DRB. As a result, virus RNA synthesized in the presence of DRB contained a peak at 3S to 7S in addition ot residual high mol. wt. virus RNA. Small virus RNA from either untreated or DRB-treated cells contained three- to sixfold higher levels of transcripts from the DNA fragment which lies between the BamHI and Bg/I sites (58 . 0 to 72 . 2 map units) than from DNA fragments covering the rest of the virus genome. Furthermore, 80% of the small RNA which hybridized to this fragment was complementary to the L strand of virus DNA. These results suggest that L strand transcripts are initiated within the Bg/I-BamHI DNA fragment and that a portion of these transcripts is prematurely terminated within several hundred nucleotides of the site(s) of initiation. DRB had little effect on the synthesis of these prematurely terminated RNAs.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000235:genetics; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology*; D005814:Genes, Viral; D007668:Kidney; D051379:Mice; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D012263:Ribonucleosides / Q000494:pharmacology*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral; D012263:Ribonucleosides; D004004:Dichlororibofuranosylbenzimidazole; D012313:RNA