The effect of rifampicin on Mycobacterium smegmatis.
Rifampicin was found to inhibit the growth and incorporation of 14C-adenine, 14C-leucine and 14C-glycine in exponentially growing cells of M. smegmatic cultivated in Merrill's synthetic medium. Increasing concentrations of the antibiotic inhibited respiration in resting cells, in the presence of glucose or 2-oxoglutarate as substrates in particular. In addition to the well-known interference of rifampicin with the biosynthesis of RNA, the effect on the energy metabolism should also be considered.
D000225:Adenine / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D005998:Glycine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D009161:Mycobacterium / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D007930:Leucine; D000225:Adenine; D005998:Glycine; D012293:Rifampin