A rapid gas liquid chromatography method for simultaneous determination of various anticonvulsants.
The purpose of this study was to develop a rapid GLC method for the simultaneous determination of the following anticonvulsants: Carbamazepine, Diphenylhydantoin, Phenobarbital, and Primidone. Using a metal column (OV-17) with flash heater methylation as well as high N2 flow-rate, a quantitative methylation of the above anticonvulsants was obtained. A selective temperature program proved to be the decisive factor in separating the various methylated anticonvulsants. The GLC program was completed within 13 min. This method permits a rapid, simultanous and quantitative determination of various important anticonvulsants.
D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D004359:Drug Therapy, Combination; D004827:Epilepsy / Q000097:blood*; D006801:Humans; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011324:Primidone / Q000097:blood
D000927:Anticonvulsants; D011324:Primidone; D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital