In vitro studies on the antifungal effect of PUVA.
The effect of PUVA therapy upon 12 dermatophyte strains, 3 of Candida albicans, and 4 of mould fungi was evaluated by the agar dilution method on Sabouraud agar. 8-MOP, 30 microgram/ml medium plus UVA, 4 Joule/cm2 proved to be fungicidal dose to all the strains studied, with the exception of three of the mould fungi. For dematophytes and Candida albicans the MIC was between 1 and 3 microgram, 8-MOP plus UVA, 4 J; and 3 microgram when the UVA dose was reduced to 1--2 J. For mould fungi it was higher. The MFC varied within the range 2 to 30 microgram, when the UVA dose was fixed at 4 J. It reached 16 J when the dose of 8-MOP was kept at 4 microgram. The wide range in the MFC of the dermatophytes could be due to resistant colonies. 8-MOP alone had a distinctly inhibitory effect upon dermatophytes, but not upon Candida albicans or mould fungi.
D003883:Arthrodermataceae / Q000187:drug effects / Q000528:radiation effects; D002176:Candida albicans / Q000187:drug effects / Q000528:radiation effects; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D008730:Methoxsalen / Q000494:pharmacology*; D014466:Ultraviolet Rays
D008730:Methoxsalen