Interferon increases HLA synthesis in melanoma cells: interferon-resistant and -sensitive cell lines.
We report that human leukocyte interferon preparations increase the expression of beta 2-microglobulin by 100-200% on the surface of normal fibroblast and melanoma cell lines sensitive to interferon. This increase in expression can be correlated with an increase in HLA synthesis as measured by incorporation of [35S]methionine in these antigens. This enhanced HLA synthesis, which is 5- to 17-fold, is time dependent and dose related. Synchronized cells in the G0/G1 phase of the cell cycle appear to be more sensitive to this interferon action. Neither an increase in surface expression nor in HLA synthesis is observed in a melanoma cell line resistant to the antiviral and antigrowth effects of interferon. Furthermore, there appears to be a stronger correlation between this increased HLA synthesis and the antiviral function than between it and the antiproliferative action of interferon.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008545:Melanoma / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D013997:Time Factors; D014762:Viral Interference / Q000187:drug effects; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000235:genetics
D006680:HLA Antigens; D001613:beta 2-Microglobulin; D007372:Interferons