Studies on the mechanisms of somatostatin release after insulin induced hypoglycaemia in man.
Insulin-induced hypoglycaemia, which stimulates gastric acid secretion, is associated with an increase in circulating somatostatin levels in man. In order to assess the mechanisms involved in this rise, six normal volunteers connected to a Biostator for continuous glucose monitoring were studied, on three separate occasions. On each occasion after basal blood sampling, 0.15 i.u./kg body weight of insulin was administered i.v. and further samples were obtained intermittently over 150 min. On one occasion, dextrose was infused by the Biostator to prevent hypoglycaemia, while on the other two, a constant infusion of either normal saline or the specific H2 antagonist cimetidine was administered. Insulin plus dextrose caused no significant changes in circulating somatostatin levels, whereas insulin plus saline was associated with a marked, sustained and significant rise in all subjects; insulin plus cimetidine also produced a rise but it was delayed; the area under the curve was significantly (P less than 0.05) greater with insulin plus saline than with insulin plus cimetidine. These results show that in man insulin itself does not stimulate somatostatin secretion directly, but indirectly via hypoglycaemia. Further, the inhibition of gastric acid secretion with cimetidine reduces somatostatin release during insulin-induced hypoglycaemia. This suggests that gastric acid may mediate somatostatin secretion associated with insulin-induced hypoglycaemia.
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002927:Cimetidine / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D007003:Hypoglycemia / Q000139:chemically induced / Q000503:physiopathology*; D007328:Insulin / Q000494:pharmacology; D008297:Male; D012636:Secretory Rate / Q000187:drug effects; D013004:Somatostatin / Q000097:blood / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; D013004:Somatostatin; D002927:Cimetidine; D013006:Growth Hormone; D005934:Glucagon; D005947:Glucose; D006854:Hydrocortisone