Evaluation of the release of mutagens from diesel particles in the presence of physiological fluids.
The Ames Salmonella typhimurium plate incorporation assay was used to evaluate the mutagenicity of organics extracted from diesel exhaust particles. Organic solvents were more efficient than physiological fluids in removing mutagens from diesel particles, with dichloromethane extracts having the greatest mutagenic activity of the solvents examined. Serum and lung cytosol were more effective than acellular lung lavage fluid in releasing mutagenic activity from diesel particles. The mutagenic activity of diesel particle organics preextracted with dichloromethane is greatly reduced upon the addition of serum and lung cytosol to organics. Subsequently, incubation of protease with serum and lung cytosol-bound diesel organics increases the mutagenic activity. Fluorescence intensity was quantitated and found to correlate with mutagenic activity in the organic and serum extracts, but not the lung cytosol extracts. These studies suggest that substantial mutagenic activity is released from diesel particles upon incubation with serum and lung cytosol.
D000818:Animals; D001711:Biotransformation; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000187:drug effects*; D008297:Male; D009153:Mutagens / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D012995:Solubility; D012997:Solvents; D013057:Spectrum Analysis; D001335:Vehicle Emissions / Q000009:adverse effects / Q000032:analysis*
D009153:Mutagens; D012997:Solvents; D001335:Vehicle Emissions