Isolation and characterization of Phycomyces blakesleeanus ferritin.
Ferritin was isolated from the fungus Phycomyces blakesleeanus and compared biochemically and immunologically with horse spleen ferritin. Phycomyces and horse spleen ferritins were shown to exhibit similar electrophoretic patterns on polyacrylamide gels. Both preparations yielded an identical single band on sodium dodecyl sulfate-containing polyacrylamide gels. Tryptic digests of Phycomyces ferritin yielded 17 ninhydrin-positive spots as compared to 26 for horse spleen ferritin tryptic digests. Phycomyces ferritin was immunologically unrelated to horse spleen ferritin.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005293:Ferritins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D005658:Fungi / Q000032:analysis*; D006736:Horses; D010455:Peptides / Q000032:analysis; D010800:Phycomyces / Q000032:analysis*; D013154:Spleen / Q000032:analysis
D000939:Epitopes; D010455:Peptides; D005293:Ferritins