Human fetal bone alkaline phosphatase: preliminary immunologic studies.
A partially purified human fetal bone alkaline phosphatase preparation (B-APf) exhibits a class of antigenic determinants not found on any adult bone or liver isoenzymes as well as a set of determinants which it shares with adult bone and liver. The evidence for this is the quantitative immunotitration data which demonstrate the expected precipitation of L-AP, B-APa, B-APf and K-AP by the anti-B-APf reagent at equivalence. However, at high antibody excess, I-AP and P-AP are also precipitated. Conversely, by employing an immunosorbent column of Sepharose-purified L-AP, one can remove the component carrying the antigenic determinants shared with L-AP, B-APa and K-AP. Remaining in solution are antibodies which recognize determinant(s) shared by B-APf, P-AP and I-AP.
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000936:Antigen-Antibody Complex; D001842:Bone and Bones / Q000201:enzymology*; D000939:Epitopes; D005260:Female; D005333:Fetus; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007700:Kinetics; D011247:Pregnancy
D000936:Antigen-Antibody Complex; D000939:Epitopes; D007106:Immune Sera; D000469:Alkaline Phosphatase