Separation of blood cells from normal blood and blood of patients with various malignant blood diseases using separation media.
Differential counts of normal and leukemic cells separated from human peripheral blood were compared using three different separation media a) Verografin, b) Verografin-Ficoll, c) Isopaque-Ficoll (Lymphoprep), density 1.077 g/ml. In the separation the solution of Verografin (SPOFA) or Verografin-Ficoll yields analogous cell counts to Isopaque-Ficoll solution. The separation of leukemic cells is similar on all three separation media with considerable packing of some cells of the myeloid line. The blood cells separated from peripheral blood of normal donors on three various separation media correlate also in T and B lymphocyte counts, as demonstrated by the results of E and EAC rosette tests.
D002469:Cell Separation / Q000379:methods*; D005362:Ficoll; D006801:Humans; D007938:Leukemia / Q000097:blood*; D012996:Solutions
D012996:Solutions; D005362:Ficoll