Spontaneous production of gamma interferon and induced production of beta interferon by human T-lymphoblastoid cell lines.
Two human T-lymphoblastoid cell lines, CCRF/CEM and Molt 4, produced beta interferon (IFN-beta) upon infection with Sendai virus. Molt 4, but not CCRF/CEM, spontaneously produced up to 300 U of IFN-gamma per ml, apparently not contaminated with IFN-alpha or -beta. Phytohemagglutinin, a T-cell mitogen, did not stimulate IFN production in these lines. A third T-lymphoblastoid line, CCRF/HSB2, produced no IFN either spontaneously or after infection with Sendai virus or treatment with phytohemagglutinin. The Molt 4 cells contained an mRNA which could be translated by oocytes to give IFN-gamma. Molt 4 cells therefore provide a convenient source of human IFN-gamma and its mRNA for experimental purposes.
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis*; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D003470:Culture Media; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D007372:Interferons; D001973:Bromodeoxyuridine