Direct identification of enteric adenovirus, a candidate new serotype, associated with infantile gastroenteritis.
Two enzyme-linked immunosorbent assay techniques for detection of adenovirus in stools were developed. The first, which is group-specific, detects the 35 established adenovirus types and, in addition, enteric adenoviruses associated with infantile gastroenteritis. The second technique, which is type-specific, selectively detects enteric adenovirus. The efficiency of these techniques was assayed on nine coded stool specimens from Glasgow children. Eight of nine was classified as adenovirus by the group-speicific enzyme-linked immunosorbent assay. The six enteric adenovirus specimens were antigenically distinct from each of the 35 established adenovirus types but not from each other. They are suggested to represent a new adenovirus serotype which appear to be associated with gastroenteritis without clear-cut respiratory symptoms.
D000260:Adenoviruses, Human / Q000145:classification* / Q000302:isolation & purification; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005243:Feces / Q000382:microbiology; D005260:Female; D005387:Finland; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008297:Male; D012703:Serotyping; D006113:United Kingdom
D000939:Epitopes