Observations on the early actions of prolactin on RNA and casein synthesis in mouse mammary gland explants.
The action of prolactin on RNA synthesis in cultured mouse mammary gland explants becomes manifest when the tissues are exposed only briefly (1 h or less) to prolactin. In contrast, the action of prolactin on casein synthesis only becomes apparent when the tissues are cultured for 6 h or more with prolactin. Once the actions of prolactin on RNA and casein synthesis are initiated, however, these effects persist for hours or days, even when the tissues are subsequently cultured in the absence of prolactin.
D000818:Animals; D002364:Caseins / Q000096:biosynthesis*; D046508:Culture Techniques; D005260:Female; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011388:Prolactin / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*
D002364:Caseins; D012313:RNA; D011388:Prolactin