A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation.
A simple one-step method for purification of human granulocytes, based on the use of a discontinuous gradient of Percoll, is described. The cell yield is 55% and the purity of the granulocyte fraction is 97%. The cells' ability to exclude trypan blue and phagocytic function are not altered during the purification process. We conclude that centrifugation in Percoll is superior to conventional dextran sedimentation; it is also less time-consuming.
D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D003102:Colloids; D003911:Dextrans; D006098:Granulocytes / Q000166:cytology* / Q000502:physiology; D006801:Humans; D010587:Phagocytosis; D011205:Povidone; D012822:Silicon Dioxide
D003102:Colloids; D003911:Dextrans; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone