title: Mouse mast cell sensitization with human IgG and allergen.
abstract: The heterologous binding of human IgG to mouse peritoneal mast cells was studied in vitro. The specific percentage of mast cell degranulation after treatment with sera heated for 2 hours at 57 degrees C was determined. These sera were obtained from normal or allergic patients, skin test positive to grass mix or dust, and then challenged separately with each allergen. The results show a high performance of degranulation by anti-human IgG and a direct correlation between skin test positivity and high degranulation values.
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D004391:Dust; D005260:Female; D006636:Histamine Release; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D006109:Poaceae; D012882:Skin Tests; D013045:Species Specificity
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D004391:Dust; D007074:Immunoglobulin G