An evaluation of serum sources used in the separation of T- and B-lymphocytes by nylon-wool columns.
Gamma-globulin-free (GG-) newborn-calf, mouse and horse, as well as newborn-calf and calf serums were evaluated as substitutes for fetal bovine serum in the classical nylon-wool procedure used for the separation of T- and B-lymphocytes. The efficacy of the cell separation was evaluated by non-specific esterase staining and cytotoxicity tests for cell surface Thy 1.2 antigen and surface immunoglobulins. The results of these studies indicate that GG- newborn-calf, mouse and horse but not newborn-calf or calf serum could be substituted for fetal bovine serum. The results of these studies also indicate that the ratio of adherent to non-adherent cells is a useful indicator of the efficacy of the cell separation. A ratio of non-adherent to adherent less than 1.0 is indicative of a successful separation.
D000818:Animals; D001402:B-Lymphocytes; D001769:Blood; D002417:Cattle; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D005069:Evaluation Studies as Topic; D006736:Horses; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009757:Nylons; D013601:T-Lymphocytes; D014935:Wool; D005719:gamma-Globulins
D009757:Nylons; D005719:gamma-Globulins