title: [Practical staining method: T-OPA triple stain].
abstract: The following procedure has proven to be successful as routine trichrome stain on paraffin embedded material: 1) Mayer's hemalum for 10 min, followed by running tap water wash; 2) staining in 1% Orange G in 1% acqueous PTA for 5 min and rinsing a few seconds in distilled water; 3) Aniline blue 1% acqueous for 5 min, followed by few seconds distilled water wash. Dehidratation in ethanol, or by blotting followed by t-buthanol or 1:3 terpineol-xylene, clearing and mounting, completed the procedure.
mesh_terms: D000165:Acridine Orange; D000814:Aniline Compounds; D010772:Phosphotungstic Acid; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000814:Aniline Compounds; D010772:Phosphotungstic Acid; D000165:Acridine Orange
vernacular_title: Un pratico metodo di colorazione: la tricromica t-opa.