Simultaneous determination of serotonin, 5-hydroxindoleacetic acid, 3,4-dihydroxyphenylacetic acid and homovanillic acid by high performance liquid chromatography with electrochemical detection.
A rapid and highly sensitive procedure for simultaneous determination of serotonin, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic acid and homovanillic acid is described. After precipitation of proteins with perchloric acid the samples are applied directly to a high performance liquid chromatograph, with electrochemical detection. As little as 20 pg of serotonin, 5-hydroxyindoleacetic acid, and 3,4-dihydroxyphenylacetic acid and 200 pg of homovanillic acid can be detected. One chromatographic run requires less than 10 min.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006719:Homovanillic Acid / Q000032:analysis*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D008832:Microchemistry; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000032:analysis*
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid