Human alpha- and beta-globin gene transcription in mouse erythroleukaemia cells.
Human beta-globin genes introduced into mouse erythroleukaemia (MEL) cells by DNA co-transformation are correctly regulated when erythroid cell differentiation is induced by dimethylsulphoxide (DMSO). In contrast, cloned human alpha-globin genes are efficiently transcribed in MEL cells before induction, and no increase in the level alpha-globin mRNA is observed when the cells differentiate. These observations suggest that the mechanisms by which alpha- and beta-globin genes are activated during erythroid cell differentiation are fundamentally different. Analysis of the transcription of hybrid human alpha-beta-globin genes in MEL cells revealed that the sequences responsible for differences in transcription of the intact alpha- and beta-globin genes are located on the 3' side of the mRNA capping site of the two genes, suggesting that cis-acting regulatory sequences are located within the structural genes.
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010957:Plasmids; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D005914:Globins; D004262:DNA Restriction Enzymes; D004121:Dimethyl Sulfoxide