Tolerance induction via antigen inhalation: isotype specificity, stability, and involvement of suppressor T cells.
Mice were exposed to aerosolized ovalbumin (OA) once weekly for 5 min on 6 occasions, prior to systemic challenge with soluble or alum-adsorbed (AH-OA) antigen. Mice challenged with AH-OA manifested profound IgE isotype-specific tolerance; those challenged with soluble OA initially manifested anamnestic IgE/IgG responses, but secondary intraperitoneal immunization 13 days later with soluble OA revealed IgE isotype-specific tolerance. The tolerized mice contained splenic suppressor T cells which inhibited IgE but not IgG responses in an adoptive transfer assay. Tolerance was still demonstrable in mice 6 months after the cessation of aerosol exposures. Exposure of low IgE responder rat strains to aerosolized OA tolerized for both IgE and IgG responses.
D000281:Administration, Intranasal; D000336:Aerosols; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D000939:Epitopes; D005260:Female; D007108:Immune Tolerance; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000336:Aerosols; D000941:Antigens; D000939:Epitopes; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D010047:Ovalbumin