Interferon treatment inhibits glycosylation of a viral protein.
Earlier, we reported a 30-200-fold reduction in the yield of infectious vesicular stomatitis virus (VSV) released from L cells treated with 10-30 reference units ml-1 of interferon (IFN); however, in these cultures virus particle production, as measured by VSV particle-associated viral RNA, virus nucleocapsid protein and viral transcriptase, was inhibited less than 10-fold. There was biochemical and morphological evidence of a significant reduction in glycoprotein (G) and membrane protein (M) of VSV particles released from IFN-treated cells. We compare here the effects of tunicamycin (TM) and IFN in L cells. Treatment with TM or IFN reduced the production of infectious VSV particles, decreased the amount of G and M proteins in VSV released from treated cells, and inhibited an early step in the formation of asparagine-linked oligosaccharide chains, the incorporation by membrane preparations from treated cells of N-acetylglucosamine into glycolipids with the properties of dolichol derivatives.
D004288:Dolichol Phosphates / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000096:biosynthesis*; D007372:Interferons / Q000494:pharmacology*; D014415:Tunicamycin / Q000494:pharmacology*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects
D004288:Dolichol Phosphates; D006023:Glycoproteins; D014764:Viral Proteins; D014415:Tunicamycin; D014537:Uridine Diphosphate N-Acetylglucosamine; D007372:Interferons; D005944:Glucosamine