Preparation of human alpha-2-macroglobulin by chromatography on Cibacron Blue Sepharose in the presence of pregnancy- associated alpha-2-glycoprotein.
The simple and efficient procedure for the isolation of alpha-2-macroglobulin (alpha-2-M) from human sera (hp-type 1-1) by means of affinity chromatography on Cibacron Blue Sepharose is not convenient to separate it from pregnancy-associated alpha-2-glycoprotein (alpha-2-PAG) which is present in high amounts in sera of estrogen-treated women, at pregnancy, and under other conditions. With this method both proteins are eluted in the same fractions; gel filtration of these fractions does not lead to their separation. Therefore, the use of male sera (tesed by monospecific antisera to alpha-2-PAG) with low alpha-2-PAG, content (hp-type 1-1) is recommended for alpha-2-M preparation.
D000873:Anthracenes; D002846:Chromatography, Affinity; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D000511:alpha-Macroglobulins / Q000302:isolation & purification*
D000873:Anthracenes; D004396:Coloring Agents; D011257:Pregnancy Proteins; D000511:alpha-Macroglobulins; C017842:cibacron blue-sepharose; D012685:Sepharose