Release of mediators from free bronchoalveolar cells: effect of ionophore A23187 and antigen challenge.
The ionophore A23187 stimulated the release of prostaglandins and SRS-A (now identified as leukotriene D4) from guinea-pig bronchoalveolar macrophages. Upon specific antigen challenge of the cells obtained from ovalbumin-sensitized animals or of normal cells preincubated with the serum of sensitized animals, prostaglandins, SRS-A or histamine were undetectable. Furthermore, treatment of "sensitized" cells with the ionophore resulted in a reduced liberation of prostaglandins in comparison to control cells. It is suggested that sensitization produce in bronchoalveolar macrophages some functional alterations of arachidonic acid oxygenation products.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000941:Antigens; D001980:Bronchi / Q000166:cytology / Q000276:immunology*; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology*; D013189:SRS-A / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000941:Antigens; D011458:Prostaglandins E; D013189:SRS-A; D000001:Calcimycin