RNA and protein synthesis in cultured human fibroblasts derived from donors of various ages.
RNA synthesis in human fibroblasts from donors of various ages was studied in fibroblasts made permeable to nucleoside triphosphates with the nonionic detergent Nonidet P40. Cells from donors of 11 years and older showed a 30-40% decline in total RNA synthesis. The decrease in RNA synthesis was primarily due to a lowering of RNA polymerase II activity (alpha-amanitin sensitive). Studies on the incorporation of leucine into protein also showed a 30-40% decrease in cells from older donors.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009711:Nucleotides / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*
D009711:Nucleotides; D011092:Polyethylene Glycols; D012313:RNA; D017830:Octoxynol; C010615:Nonidet P-40; D007930:Leucine