Monoclonal and allospecific H-2Kk antisera precipitate a high molecular weight protein.
A protein with an apparent m.w. of 68,000 has been observed in immunoprecipitations of NP-40 solubilized BW5147 thymoma cells by using monoclonal or polyclonal allospecific H-2Kk antisera. By contrast, H-2Dk alloantisera precipitated a 45,000 m.w. protein from the same membrane preparations. The 68,000 m.w. protein is expressed on the plasma membrane as determined by vectoral labeling. Extensive clearing experiments and the use of monoclonal antibody demonstrate that the protein does not bear group-specific C-type viral determinants.
D000818:Animals; D011232:Chemical Precipitation; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens; D007519:Isoantigens; D008565:Membrane Proteins; D051379:Mice; D008806:Mice, Inbred AKR; D008970:Molecular Weight; D011506:Proteins
D000939:Epitopes; D006183:H-2 Antigens; D007519:Isoantigens; D008565:Membrane Proteins; D011506:Proteins