[Model experiments for quantitative cytophotometry using protein films (author's transl)].
The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D006651:Histocytochemistry / Q000379:methods*; D011506:Proteins; D012015:Reference Standards; D013053:Spectrophotometry; D013194:Staining and Labeling
D011506:Proteins
Modellversuche zur quantitativen Cytophotometrie mit Proteinfilmen.