Translation of mRNA for human lymphotoxin in microinjected Xenopus oocytes.
Synthesis and secretion of biologically active human lymphotoxin (LT) can be detected in Xenopus laevis oocytes following their inoculation with poly(A+) RNA from human stimulated peripheral blood lymphocytes, but not in oocytes inoculated with RNA from unstimulated lymphocytes or from fibroblastoid cells. In size-fractionating mRNA of stimulated lymphocytes most LT activity is found to be coded for by RNA with an approximate sedimentation value of 19 S.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008233:Lymphotoxin-alpha / Q000096:biosynthesis* / Q000494:pharmacology; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011061:Poly A / Q000008:administration & dosage / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000008:administration & dosage / Q000378:metabolism*; D014982:Xenopus laevis
D008233:Lymphotoxin-alpha; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D011061:Poly A