[Degradation of glutamic acid and proline in Clostridium sordellii under cadaveric bacteriological inspection].
Glutamic acid (GLU) is decarboxylated to gamma-aminobutyric acid (GABA) by Clostridium sordellii isolated from a decaying human brain. The dependence of this reaction on temperature, pH and substrate concentration has been established. The pH-optimum is in the range of 5.0 to 5.2. Near optimal pH the temperature optimum is greater than 28 degrees C. At higher pH-values (6 to 7) activity is relatively independent of temperature. The similarity to results in decaying human brains (10, 11, 12) shows a good correlation between postmortem bacterial flora and GLU-degradation. Furthermore it is shown that C. sordellii produces delta-aminovaleric acid (AVA) from proline (PRO).
D000596:Amino Acids / Q000096:biosynthesis; D021542:Amino Acids, Neutral; D001921:Brain / Q000382:microbiology*; D002102:Cadaver; D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D003653:Decarboxylation; D005554:Forensic Medicine; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011180:Postmortem Changes; D011392:Proline / Q000378:metabolism; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis
D000596:Amino Acids; D021542:Amino Acids, Neutral; D003470:Culture Media; D005971:Glutamates; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; C013809:5-aminovaleric acid; D011392:Proline
Der Abbau von Glutaminsäure und Prolin in Clostridium sordellii unter leichenbakteriologischen Aspekten.