Expression of the human fibroblast interferon gene in Escherichia coli.
We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T.M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. We have detected no such activity in extracts of bacteria synthesizing pre-F-IF.
D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D007372:Interferons / Q000096:biosynthesis / Q000235:genetics*; D008722:Methods; D009876:Operon; D010957:Plasmids; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D004274:DNA, Recombinant; D007372:Interferons