A reliable Feulgen-acriflavine-SO2 staining procedure for quantitative DNA measurements.
Feulgen-acriflavine-SO2 staining was performed on chicken erythrocytes and rat liver cells under different cytochemical conditions. The obtained results were compared to conventional Feulgen-pararosaniline-SO2 staining by means of scanning cytophotometry and microfluorometry. It was found that if acid ethanol rinsing was included in the Feulgen-acriflavine-SO2 procedure, both Feulgen procedures were fully comparable in specificity and quantitative aspects.
D000167:Acriflavine; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002645:Chickens; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006868:Hydrolysis; D008099:Liver / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling; D013458:Sulfur Dioxide
D013458:Sulfur Dioxide; D000167:Acriflavine; D004247:DNA