Large scale production of mouse interferons from monolayers of Ehrlich ascites tumour cells.
Conditions are described for the large scale production of mouse interferons from Ehrlich ascites tumour cells cultured as monolayers in roller bottles. With the procedure reported here, we have used 50 to 65 600 cm(2) roller bottles to produce routinely 2 X 10(9) to 3 X 10(9) International units of crude mouse interferon/week with a specific activity of 1 X 10(6) to 1.5 X 10(6) units/mg protein.
D000818:Animals; D001769:Blood; D002286:Carcinoma, Ehrlich Tumor; D002460:Cell Line; D003470:Culture Media; D007372:Interferons / Q000096:biosynthesis* / Q000302:isolation & purification; D008722:Methods; D051379:Mice
D003470:Culture Media; D007372:Interferons