Vasoconstrictor responses induced by alpha-adrenoreceptor agonists before and after removal of the endothelial cells of dog mesenteric arteries.
The stainless cannula inserting method was used to investigate the effects of alpha-adrenoreceptor agonists (phenylephrine, alpha 1-agonist and xylazine alpha 2-agonist) and calcium chloride on isolated and perfused dog mesenteric arteries with and without saponin treatment. By intraluminal administration, phenylephrine and calcium chloride caused vasoconstriction in a dose-related manner whilst xylazine was without significant effect. After intraluminal treatment with 3 mg of saponin, the endothelia of the arteries were completely removed as confirmed by pathological examinations. Phenylephrine- and xylazine-induced responses were not influenced by saponin treatment whereas calcium chloride-induced constriction was significantly enhanced.
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D004285:Dogs; D004727:Endothelium / Q000502:physiology; D005260:Female; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000502:physiology; D010656:Phenylephrine / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects*; D014991:Xylazine / Q000494:pharmacology
D000316:Adrenergic alpha-Agonists; D012503:Saponins; D010656:Phenylephrine; D014991:Xylazine; D002122:Calcium Chloride