Immortalization of antigen specific, helper T cell lines by transformation with the radiation leukemia virus (RadLV).
Antigen-specific immune T lymphocytes of male C57BL/6 mice were enriched in vitro on monolayers of antigen-pulsed syngeneic macrophages. The cells were treated in vitro with RadLV and inoculated intrathymically into irradiated female C56BL/6 animals. Thymomas arising in the inoculated recipients were characterized as donor- (male) type according to their karyotype. In vivo and in vitro cell lines were established from the primary lymphomas, two of which (designated ROT/6.1 and ROT/6.2) were capable of providing antigen- (carrier) specific help in normal or preimmunized mice. None of the lymphomas could induce antigen-specific DTH reaction. Five months after their establishment, ROT/6.2 alone retained its carrier specificity. ROT/6.2 consisted mainly of Lyt-1+ cells, whereas the ROT/6.1 population was more heterogeneous and contained Lyt-1+, Lyt-2+, and Lyt-3+ cells. The carrier specificity of the latter may have been lost due to selection against the specific helper cells during prolonged passages.
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics; D002460:Cell Line; D002472:Cell Transformation, Viral; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D005260:Female; D006580:Genetic Carrier Screening; D006968:Hypersensitivity, Delayed / Q000276:immunology; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000276:immunology*; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology; D007953:Leukemia, Radiation-Induced / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013945:Thymoma / Q000235:genetics / Q000276:immunology
D000950:Antigens, Ly; D004136:Dinitrobenzenes; D000939:Epitopes; D010047:Ovalbumin