The small nuclear RNAs of the cellular slime mold Dictyostelium discoideum. Isolation and characterization.
Three species of small nuclear RNA from the lower eucaryote Dictyostelium discoideum have been isolated and characterized with regard to size, cellular abundance, modified nucleotide content, and 5'-end structures. Previous studies had shown that the nuclei of mammalian cells contain a number of discrete low molecular weight, nonribosomal, nontransfer RNA molecules known as small nuclear RNAs. The mammalian small nuclear RNAs range in size from approximately 100 to 250 nucleotides and are quite abundant, in some cases approaching ribosomal RNA in number of copies/cell. Some of these molecules have an unusual cap structure at their 5'-ends similar to that found on eucaryotic messenger RNAs, and a number contain a characteristic set of internal modifications as well. Our results indicate that the small nuclear RNAs of Dictyostelium resemble their counterparts in higher eucaryotic cells structurally, but are present in significantly fewer copies/cell. The implications of these findings for small nuclear RNA function are discussed.
D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis*; D004023:Dictyostelium / Q000032:analysis*; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D012313:RNA / Q000302:isolation & purification*; D012334:RNA, Neoplasm / Q000032:analysis; D006163:Ribonuclease T1
D012334:RNA, Neoplasm; D012313:RNA; D006163:Ribonuclease T1