[Biosynthesis of carbohydrate-containing polymers in plants. I. Products formed with enzyme preparation from clover seedlings. Characterization of polyprenylphosphosugars].
Membrane preparations from clover seedlings catalyzed the incorporation of monosaccharide residues from GDPMan, UDPGlc and UDPGal into glycolipids, lipid-oligosaccharides and polymers. The lipid-oligosaccharides were shown to be alpha-dihydropolyprenyl pyrophosphate derivatives. Incorporation of mannose residues into the lipid-oligosaccharides and the polymers was significantly stimulated by addition of UDPGlc, GDPGlc, UDPGal but not by UDPGlcNAc. Exogenic polyprenyl phosphates also stimulated the process; the formation of moraprenyl pyrophosphate oligosaccharides was demonstrated after addition of moraprenyl phosphate. The lipid-oligosaccharides were precursors of the polymers which were shown to be mainly glycoproteins. A solubilized preparation of mannosyl transferase from clover membranes was obtained and some properties of the enzyme were studied.
D006017:Glycolipids / Q000096:biosynthesis; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis; D010944:Plants / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis
D006017:Glycolipids; D009844:Oligosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D011134:Polysaccharides; D006155:Guanosine Diphosphate Mannose; D008364:Mannosyltransferases
Biosintez rastitel'nykh uglevodsoderzhashchikh polimerov. Produkty, obrazuiushchiesia s preparatom fermentov iz prorostkov klevera. Kharakteristika poliprenilfosfosakharov.