The biliary excretion of trypsin in rats.
The serum half-life of bovine [3H]acetyltrypsin was estimated to be 9 min following intravenous administration in rats. This was maintained when six successive doses of 200 micrograms each were given at 1-h intervals. The enzyme was removed from the circulation after complexing with alpha 2-macroglobulin (alpha 2-M). The amount of 3H label appearing in bile increased with each successive dose and this was associated with breakdown products ( less than 10,000 daltons) of the alpha 2-M/[3H]acetyltrypsin. Intact alpha 2-M/[3H]acetyltrypsin was recovered from bile but represented only 0.06% of the administered dose of active enzyme.
D000107:Acetylation; D000818:Animals; D001646:Bile / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006207:Half-Life; D008099:Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014357:Trypsin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism
D000511:alpha-Macroglobulins; D014316:Tritium; D014357:Trypsin