beta-Aminopropionitrile prevents bleomycin-induced pulmonary fibrosis in the hamster.
beta-Aminopropionitrile (beta APN), an agent that prevents collagen accumulation in tissues, was evaluated for its ability to prevent excess collagen formation in bleomycin-induced pulmonary fibrosis in the hamster. Two groups of animals received a single endotracheal dose of bleomycin; one of these was injected with beta APN twice daily for 30 days. A third group received endotracheal saline and a fourth group received saline and beta APN. After 30 days, we measured pressure-volume curves of saline-filled lungs, collagen content, and degree of fibrosis. Endotracheal bleomycin increased collagen content, decreased lung volume, and produced fibrosis and a mortality rate of 51%. The administration of beta APN to bleomycin-treated animals prevented excess collagen accumulation and diminished total protein, reversed volume diminution, produced less fibrosis, and improved the mortality rate to 24%; beta APN alone had no effect on lung mechanics or collagen content. The biochemical, functional, and structural features of bleomycin-induced lung fibrosis are amenable to control with beta APN.
D000629:Aminopropionitrile / Q000627:therapeutic use*; D000818:Animals; D001761:Bleomycin; D001835:Body Weight; D003094:Collagen / Q000096:biosynthesis; D006224:Cricetinae; D008168:Lung / Q000473:pathology; D008176:Lung Volume Measurements; D008297:Male; D009929:Organ Size; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
D001761:Bleomycin; D000629:Aminopropionitrile; D003094:Collagen