Lipopolysaccharide induces retroviral antigen expression in 129/J mouse lymphocytes: evidence for assembly of a defective viral particle.
In contrast to those of many other mouse strains, spleen cell cultures of 129/J mice do not release reverse transcriptase activity into the supernatant upon stimulation with bacterial lipopolysaccharide. We report here that lipopolysaccharide induced the expression of intracellular viral proteins in 129/J spleen cells. Furthermore, we found that stimulated spleen cells released retroviral particles. We conclude that 129/J mice are inducible with lipopolysaccharide but that the virus produced is a defective particle deficient in reverse transcriptase activity.
D000818:Animals; D000956:Antigens, Viral; D002478:Cells, Cultured; D003673:Defective Viruses / Q000254:growth & development*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000382:microbiology*; D051379:Mice; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000254:growth & development* / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis*
D000956:Antigens, Viral; D008070:Lipopolysaccharides; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase