Haemagglutination-enhancing factor protein in chicken serum. I. Purification of an active protein and production of a monospecific antiserum against it.
The haemagglutinating activity of the IgG fraction of chicken alloantibodies is greatly enhanced by the addition of normal chicken serum. The effect is due to a macromolecular beta-globulin, which has been purified by salt precipitation, gel filtration, and ion-exchange chromatography. Immunization of rabbits with the purified product gave a monospecific antiserum against the haemagglutination-enhancing factor (HEF) protein. By means of this rabbit antiserum it is shown that HEF is co-precipitated with antigen-antibody complexes. This indicates that HEF exerts its effect by binding to chicken IgG antibody after the latter has reacted with antigen.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001609:Beta-Globulins / Q000276:immunology* / Q000302:isolation & purification; D002645:Chickens; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006384:Hemagglutination; D007106:Immune Sera; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007518:Isoantibodies / Q000032:analysis; D008297:Male; D011817:Rabbits
D001609:Beta-Globulins; D007106:Immune Sera; D007074:Immunoglobulin G; D007518:Isoantibodies