Application of colloidal iron method to semithin sections of epoxy embedded tissues.
Hale's colloidal iron reaction for acid mucopolysaccharides was applied to semithin sections of Epon-embedded tissues routinely processed for electron microscopy. The reaction was found to be positive only after partial removal of the resin in NaOH/absolute ethanol solution; its sensitivity, however, did not reach that obtained in paraffin or frozen sections. The duration of the Epon-removing procedure seems to be crucial for the specificity of staining.
D000818:Animals; D001001:Anura; D002356:Cartilage / Q000166:cytology; D003102:Colloids; D004853:Epoxy Resins; D005629:Frozen Sections; D006025:Glycosaminoglycans / Q000032:analysis*; D007421:Intestine, Small / Q000166:cytology; D007501:Iron; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008722:Methods; D051379:Mice; D008867:Microtomy; D010902:Pituitary Gland / Q000166:cytology; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013363:Submandibular Gland / Q000166:cytology; D014132:Trachea / Q000166:cytology
D003102:Colloids; D004853:Epoxy Resins; D006025:Glycosaminoglycans; D007501:Iron