Surface immunoglobulin receptors of rabbit lymphoid cells. Evaluation of fluorescent staining with antibodies to immunoglobulin light chain allotypes.
Rabbit lymphoid cells were stained with fluorescein-labelled antiallotype antibodies. The double layer technique was found to be more sensitive than the direct staining. Rabbit B cells are stained only via their surface immunoglobulin (sIg) receptors and not via the receptors for the Fc portion of the IgG. Peritoneal exudate macrophages do not carry sIg receptors and are stained via their Fc receptors. Removal of protein aggregates from the system and use of reagents prepared from F(ab')2 immunoglobulin fragments prevent staining of the macrophages through their Fc receptors. There was a good agreement between the percent of RABELA-positive and sIg-containing spleen cells. In appendix there were approximately 20% more RABELA-positive than sIg-positive cells.
D000818:Animals; D000918:Antibody Specificity; D001065:Appendix / Q000166:cytology; D001402:B-Lymphocytes / Q000032:analysis*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling
D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D011971:Receptors, Immunologic