Angiogenesis in vitro.
Cloned capillary endothelial cells, cultured in tumour-conditioned medium, form capillary tubes. By light and electron microscopy these tubes resemble capillaries in vivo. This first demonstration of angiogenesis in vitro: (1) shows that all the information necessary to develop an entire capillary network in vitro is expressed by one cell type; (2) suggests a mechanism for lumen formation; and (3) offers a possibility of distinguishing between direct and indirect angiogenesis factors.
D043925:Angiogenesis Inducing Agents / Q000032:analysis; D002196:Capillaries / Q000254:growth & development* / Q000648:ultrastructure; D002449:Cell Aggregation; D002478:Cells, Cultured; D004727:Endothelium / Q000502:physiology*; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic; D014617:Vacuoles / Q000648:ultrastructure
D043925:Angiogenesis Inducing Agents