Serological analysis of B6.C-H-2bm12 (I-A mutant).
The B6.C-H-2bm12 has been examined serologically with a new set of reagents and several complementation studies were performed to determine the extent of the mutation. The results show that: (a) the mutation has also affected the site(s) bound by xenogeneic anti-Ia antibodies; (b) the IJb region was not affected; (c) complementation studies with stains bearing a, b, d, k and s haplotypes did not complement bm12 for the expression of the lost I-Ab specificities, suggesting a structural (rather than regulatory) gene alteration in bm12; (d) H-2 haplotypes b and bm12 could complement d to establish the Ia.22 specificity, indicating that Ia-1 and Ae are separate genes in the I-A subregion. In addition, an antibody to the gained specificity on bm12 is described.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000235:genetics*; D005260:Female; D005816:Genetic Complementation Test; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006824:Hybridization, Genetic; D008297:Male; D051379:Mice; D008805:Mice, Inbred A / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D012397:Rosette Formation; D012699:Serology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000949:Histocompatibility Antigens Class II