The DNA content of human plasma cells.
The DNA content of human plasma cells from myeloma patients relative to that of leukocytes was determined by flow and microscopic cytofluorometry after propidium iodide and fluorescent Feulgen staining, respectively. Mononucleated myeloma plasma cells from all of the 17 patients studied contained more DNA (17 to 58%) than the leukocytes from the patient. The binucleated and trinucleated plasma cells, which were more prevalent in advanced cases, contained up to two and three times, respectively, the amount of DNA determined in the mononucleated plasma cells. These observations suggest that the ploidy abnormalities of myeloma plasma cells are even more extensive than the numerous karyotypic studies have indicated.
D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis*; D005470:Fluorometry; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D009101:Multiple Myeloma / Q000378:metabolism*; D010950:Plasma Cells / Q000032:analysis*; D013194:Staining and Labeling
D004247:DNA