Interferon induction by viruses. Sindbis virus: defective-interfering particles temperature-sensitive for interferon induction.
A defective-interfering (DI) particle of Sindbis virus was generated from a ts mutant of RNA-complementation group A by serially undiluted passages at 30 degrees C. This mutant induced interferon at a permissive temperature (30 degrees C), but not at a non-permissive temperature (40.5 degrees C); it also expressed homotypic interference throughout the range 30 to 40.5 degrees C. This demonstrates for the first time in a DI particle a ts function, namely, the ability to induce interferon. In addition, our data provide further evidence that the RNA genome of a Sindbis DI particle can be translated within the cell. We postulate that the products of translation function to produce the putative inducer of interferon, namely a molecule of dsRNA.
D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D005814:Genes, Viral; D007372:Interferons / Q000096:biosynthesis*; D009154:Mutation; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D012845:Sindbis Virus / Q000235:genetics / Q000502:physiology*; D013696:Temperature; D014762:Viral Interference
D012367:RNA, Viral; D007372:Interferons