The primary role of lymphoreticular cells in the mediation of host responses to bacterial endotoxim.
Mice that are unresponsive to lipopolysaccharide (LPS) (strain C3H/HeJ) can be rendered LPS-sensitive by the adoptive transfer of bone marrow cells from LPS-sensitive mice (strain C3H/HeN). This model of adoptive transfer was used to evaluate the contribution of lymphoreticular cells to five effects of endotoxin on the host: immunogenicity, adjuvanticity, lethality, induction of interferon, and induction of colony-stimulated factor. C3H//HeJ mice became sensitive to each of these effects after adoptive transfer of bone marrow cells from C3H/HeN mice. The efficacy of transfer was directly proportional to the dose of X-irradiation and inversely proportional to the number of surviving host stem cells. The most effective dose of radiation was 850 rad, and C3H/HeN leads to C3H/HeJx chimeras prepared at this dose were as sensitive to LPS for each parameter tested as were the C3H/HeN donors except for a threefold greater resistance to lethality than LPS-responsive C3H/HeN mice. C3H/HeN mice could also be rendered unresponsive to LPS by the adoptive transfer of C3H/HeJ bone marrow cells. C3H/HeN chimeras were resistant to all of the effects of LPS studied except for the induction of colony-stimulating factor. These results demonstrate that lymphocytes and/or macrophages play a primary role in mediating a number of diverse and seemingly unrelated host responses to endotoxin.
D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology*; D002678:Chimera / Q000528:radiation effects; D003115:Colony-Stimulating Factors / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000528:radiation effects; D007111:Immunity, Cellular; D007372:Interferons / Q000097:blood; D007928:Lethal Dose 50; D051379:Mice; D012157:Mononuclear Phagocyte System / Q000166:cytology* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D003115:Colony-Stimulating Factors; D011135:Polysaccharides, Bacterial; D007372:Interferons