Specificity of H-2-linked Ir gene control in mice: demonstration of T helper cells recognizing branched synthetic polypeptides in low responder mice.
This report provides evidence for the presence of T helper cells capable of recognizing the polypeptide antigens T6-A--L and (H,G)-A--L in low responder mice of H-2k and H-2b haplotypes, respectively. Mice were primed in vivo with the T6-A--L-avidin-(H,G)-A--L complex or, in the case of T6-A--L in H-2k mice, with the cross-reactive and permissive antigen T6-S--L. T helper cells cooperating with DNP-primed B cells could be rechallenged in vitro with the DNP-conjugates of T6--A--L or (H,G)-A--L, although the cells were of low responder type with respect to these antigens. This implies that T cell-macrophage interaction required for restimulation is apparently not defective in these low responders. The implications of these results for the concept of Ir gene control are discussed.
D000486:Allergy and Immunology; D000818:Animals; D001360:Avidin / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005802:Genes, MHC Class II; D006183:H-2 Antigens; D008285:Major Histocompatibility Complex; D051379:Mice; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D010455:Peptides; D001360:Avidin; C021759:(T,G)-A-L