Forming and removing stain precipitates on ultrathin sections.
Stain precipitates resulting from the use of lead or uranyl salts, or both, on ultrathin sections can be classified as belonging to one of three morphological types: I) extremely electron-dense particles caused by prolonged use of lead salts only, II) amorphous networks formed following double staining with either aqueous or alcoholic uranyl and lead salts, and III) crystalline needles sometimes resulting from double staining with alcoholic uranyl and lead salts. It has been found, however, that either acetic acid or aqueous uranyl acetate can be used to remove type I and type II precipitates from sections, and that oxalic acid and alcoholic uranyl solution will remove type II precipitates. Unfortunately, type III precipitates are unaffected by any agents tested so far.
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D011232:Chemical Precipitation; D002951:Citrates; D019343:Citric Acid; D000431:Ethanol; D007854:Lead; D000432:Methanol; D008974:Mollusca / Q000648:ultrastructure; D009942:Organometallic Compounds; D010070:Oxalates; D019815:Oxalic Acid; D010944:Plants / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods*; D014501:Uranium
D000085:Acetates; D002951:Citrates; D009942:Organometallic Compounds; D010070:Oxalates; C005460:uranyl acetate; D019343:Citric Acid; D007854:Lead; D000431:Ethanol; D014501:Uranium; D019815:Oxalic Acid; D019342:Acetic Acid; D000432:Methanol