Amylase secretion induced by histamine from guinea-pig parotid gland.
The mode of action of histamine and its analogues on the secretion of amylase from guinea-pig parotid gland was studied in vitro using chopped parotid tissue. Histamine, 4-methylhistamine and 2-(2-pyridyl)ethylamine dose-dependently stimulated the secretion of amylase in the presence of 3-isobutyl-1-methylxanthine. The effect of histamine was blocked by mepyramine but not by metiamide. Propranolol inhibited the effect of higher concentrations of histamine and 2-(2-pyridyl)ethylamine. A combination of mepyramine and propranolol markedly suppressed the effect of histamine and 2-(2-pyridyl)ethylamine. Atropine, phentolamine and tetrodotoxin had no influence on the histamine-induced secretion of amylase.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008761:Methylhistamines / Q000494:pharmacology; D008785:Metiamide / Q000494:pharmacology; D010306:Parotid Gland / Q000201:enzymology*; D011433:Propranolol / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology; D013268:Stimulation, Chemical; D013997:Time Factors
D008761:Methylhistamines; D008785:Metiamide; C025566:4-methylhistamine; D001285:Atropine; D006632:Histamine; D011433:Propranolol; D000681:Amylases; D011738:Pyrilamine; D015056:1-Methyl-3-isobutylxanthine