Plasma myelin basic protein assay using Gilford enzyme immunoassay cuvettes.
The assay of myelin basic protein in body fluids has potential clinical importance as a routine indicator of demyelination. Preliminary details of a competitive enzyme immunoassay for this protein have previously been published by the author (Groome, N. P. (1980) J. Neurochem. 35, 1409-1417). The present paper now describes the adaptation of this assay for use on human plasma and various aspects of routine data processing. A commercially available cuvette system was found to have advantages over microtitre plates but required a permuted arrangement of sample replicates for consistent results. For dose interpolation, the standard curve could be fitted to a three parameter non-linear equation by regression analysis or linearised by the logit/log transformation.
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D004676:Myelin Basic Protein / Q000097:blood*; D013053:Spectrophotometry / Q000295:instrumentation
D004676:Myelin Basic Protein