Soluble tumor-associated markers in lung cancer extracts.
Three lung tumor-associated markers (LTAM), previously identified as a Cohn Fraction IV alpha-globulin (LTAM-1), ferritin (LTAM-2) and lactoferrin (LTAM-3) were separated by a combination of ion exchange, dye-affinity and molecular sieve chromatography. They were further purified by polyacrylamide gel electrophoresis and antisera were raised. Analysis of human extracts by immunodiffusion showed that 80% of lung tumor extracts were positive for all three markers. Similarly, 70% of extracts from other tumors wre positive for LTAM 1, but only 10% of these extracts were positive for LTAM 2 and 3. Variation in concentration of LTAM 2 and 3 among several extracts was determined by a quantitative enzyme immunoassay. Analysis of a select group of extracts for carcinoembryonic antigen (CEA), alpha-fetoprotein and beta 2-microglobulin showed 50% of these extracts to have markedly elevated levels of CEA. The results suggest that ferritin, lactoferrin and CEA offer promise as markers for lung cancer.
D000510:Alpha-Globulins / Q000302:isolation & purification*; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000302:isolation & purification*; D006801:Humans; D005779:Immunodiffusion; D007781:Lactoferrin / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000302:isolation & purification*; D008175:Lung Neoplasms / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000302:isolation & purification; D001613:beta 2-Microglobulin / Q000302:isolation & purification
D000510:Alpha-Globulins; D002272:Carcinoembryonic Antigen; D007782:Lactoglobulins; D000509:alpha-Fetoproteins; D001613:beta 2-Microglobulin; D005293:Ferritins; D007781:Lactoferrin