The effect of prolonged fasting on total lipid synthesis and enzyme activities in the liver of the European eel (Anguilla anguilla).
The extent of fatty acid synthesis from [1-14C]acetate in liver slices was reduced 6-fold when eels were fasted for 1-7 weeks and 20-fold when fasted for 39 weeks; thereafter hepatic lipogenesis seemed to remain constant for up to 95 weeks of fasting. After a 1-3 week fast some hepatic enzyme activities were reduced (acetyl-CoA carboxylase decreased 2-fold and fatty acid synthetase declined 5-fold), while others remained unchanged (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, alpha-glycerol phosphate dehydrogenase as well as malic enzyme and ATP-citrate lyase). The optimum temperature for measuring both total lipid synthesis and lipogenic enzyme activity in eel liver was found to be 30 degrees C.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000811:Anguilla; D000818:Animals; D005215:Fasting; D064429:Fatty Acid Synthases / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis
D000085:Acetates; D008055:Lipids; D010743:Phospholipids; D005993:Glycerolphosphate Dehydrogenase; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases; D001275:ATP Citrate (pro-S)-Lyase; D000103:Acetyl-CoA Carboxylase; D019342:Acetic Acid