title: Characterization of the association of two small molecular weight RNAs with eukaryotic polysomes.
abstract: Previous studies of the adult rat cerebral cortex have shown that two of the small molecular weight RNAs, K and L, are primarily cytoplasmic in location and are present in significant quantities in the microsomal fraction. We have examined their appearance in microsomal preparations. The results demonstrate that both K and L are, in fact, associated with polyribosomes. In particular, it is shown that (i) K and L are present in both membrane-bound and free polysomes; (ii) removal of microsomal membranes with Triton X-100 does not alter their association with microsomal polysomes; (iii) all of the K and almost one-half of the L associated with rat liver polysomes are resistant to high salt washing; and (iv) K and L are present in rabbit reticulocyte-free polysome preparations. The protein synthesis inhibitors cycloheximide and aurintricarboxylic acid significantly alter the association of K and L with rabbit reticulocyte-free polysomes. It is concluded that K and L are transiently associated with polysomes during the process of protein synthesis.
mesh_terms: D000375:Aging; D000818:Animals; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000032:analysis* / Q000254:growth & development; D005260:Female; D008861:Microsomes / Q000032:analysis; D008970:Molecular Weight; D011132:Polyribosomes / Q000032:analysis* / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; D012313:RNA