[Hemoglobins, XXXIV. "Hemoglobin Heide", a new embryonal hemoglobin. N-terminal sequence of the theta-chains (author's transl)].
A new embryonic hemoglobin is described. Erythrocytes of pig embryos were lysed. The lysate was analyzed by gel-electrophoresis and the hemoglobins were separated on DEAE-Sephacel. The hemoglobin fractions were characterized by sequence analysis. We found in addition a new hemoglobin chain--theta-chain--not yet described. The primary structure of the theta-chains is similar to that of the epsilon-chains. There are only 3 differences in the first 30 residues. Furthermore, these theta-chains were found to be associated with theta-like chains and therefore we propose a hemoglobin of the type x2/theta 2 and we suggest the name "Hemoglobin Heide" for this new embryonic hemoglobin. In conclusion, four different globin gens (alpha-, epsilon-, theta- and theta-gens) and three different hemoglobins (Gower I, Gower II and Hb Heide) were detected in the embryonic respiration of mammals.
D000595:Amino Acid Sequence; D000818:Animals; D004622:Embryo, Mammalian; D004912:Erythrocytes / Q000032:analysis; D005319:Fetal Hemoglobin; D046911:Macromolecular Substances; D013552:Swine
D046911:Macromolecular Substances; C026698:hemoglobin Heide; D005319:Fetal Hemoglobin
Hämoglobine, XXXIV. "Hämoglobin Heide", ein neues embryonales Hämoglobin N-terminale Sequenz der zeta-Ketten.