The loss of sensitization of mouse and rat mast cells by acid pH treatment.
Short incubation of sensitized mouse and rat peritoneal mast cells at 37 degrees C in well defined acid pH (3.5 for mouse and 4.5 for rat mast cells) induces the loss of sensitization, as indicated by antigen-induced histamine release. The acid pH pretreatment of normal mouse and rat mast cells seems to facilitate their subsequent sensitization with mouse IgE antibodies. The possibility, that acid pH treatment removes non-specific IgE from the cells and thus facilitates the binding of specific IgE antibodies is discussed.
D000818:Animals; D000941:Antigens / Q000276:immunology; D005260:Female; D006636:Histamine Release; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D051381:Rats
D000941:Antigens; D007073:Immunoglobulin E