title: RNA synthesis in cultured human placenta.
abstract: The in vitro synthesis of RNA in the human placental tissue, incubated in organ culture, was investigated. We followed the synthesis of the poly A(-) and poly A(+) RNA fractions, and investigated the distribution of the newly synthesized RNA among the subcellular fractions isolated from first and third trimester placentas. The poly A(-) RNA was the major fraction of the RNA synthesized in vitro. The incorporation of [3H]uridine into the poly A(+) RNA fraction was very low. As protein synthesis occurred during the entire incubation period, we suggest the presence of a pool of mRNA molecules in the form of mRNP particles.
mesh_terms: D004247:DNA / Q000378:metabolism; D005260:Female; D006801:Humans; D009924:Organ Culture Techniques; D010539:Permeability; D010920:Placenta / Q000378:metabolism*; D011061:Poly A / Q000096:biosynthesis*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger; D013347:Subcellular Fractions / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D013936:Thymidine; D014529:Uridine