A monoclonal antibody detecting unusual Thy-1 determinants.
20-10-5S is a monoclonal antibody produced by the fusion of C3H anti-C3H.SW splenocytes with the SP2/0 cell line. The antibody appears to react with Thy-1 determinants by several criteria including cytotoxicity patterns, functional assays, genetic analyses, and competitive binding experiments. However, the antibody and the determinants it detects are unusual in that: 1) 20-10-5S is autoreactive; 2) the antibody shows allospecificity for Thy-1.2 vs Thy-1.1 antigens only on peripheral lymphocytes and not on thymocytes; and 3) the antibody reacts only with determinants on murine T cells and not with antigens on brain tissue or on rat thymocytes. It therefore seems that 20-10-5S reacts with murine T cell-specific Thy-1 determinants that are lost or modified during maturation of the cells on which they are expressed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D001667:Binding, Competitive; D001921:Brain / Q000276:immunology; D003348:Cortisone / Q000494:pharmacology; D000939:Epitopes; D006183:H-2 Antigens / Q000276:immunology; D007075:Immunoglobulin M; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D051381:Rats; D011916:Rats, Inbred F344; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D006183:H-2 Antigens; D007075:Immunoglobulin M; D008565:Membrane Proteins; D018800:Thy-1 Antigens; D003348:Cortisone