A double-antibody assay for grass pollen-specific IgG in human serum.
A double-antibody radioimmunoassay for measurement of grass pollen antigen-specific IgG in serum is described. Grass pollen antigens were used to show a correlation between the results obtained by this method and those obtained by measuring blocking antibodies by inhibition of antigen-induced leukocyte histamine release. The new technique described is convenient, sensitive, specific and reproducible and can be recommended for clinical use.
D000818:Animals; D000939:Epitopes; D006636:Histamine Release; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D013997:Time Factors
D000939:Epitopes; D007074:Immunoglobulin G