The surface coat on human corneal endothelium.
Scanning electron microscopy of human cadaver corneas revealed a selective binding of ruthenium red-osmium tetroxide to some substance coating the posterior endothelial surface. A coating material was not found on autolyzed cells, on denuded areas of the membrane of Descemet, or on the anterior surface of endothelial cells. Partial digestion of the coating material by urokinase and trypsin suggests the presence of at least three different structural or chemical elements.
D002102:Cadaver; D003315:Cornea / Q000378:metabolism / Q000648:ultrastructure*; D004727:Endothelium / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009993:Osmium Tetroxide / Q000378:metabolism; D012430:Ruthenium Red / Q000378:metabolism
D012430:Ruthenium Red; D009993:Osmium Tetroxide