Monoclonal antibodies against human acid alpha-glucosidase.
Acid alpha-glucosidase purified from human placenta was used to immunize a mouse (strain Balb/cHeA) according to a procedure described earlier (Stähli, C., Staehlin, T., Miggiano, V., Schmidt, J. and Häring, P. (1980) J. Immunol. Methods 32, 297-304). After fusion of spleen cells with myeloma cells, about 10% of the hybrid clones obtained produced antibodies against acid alpha-glucosidase. Finally, eight stable clones producing antibodies against the enzyme were obtained. When purified acid alpha-glucosidase is analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, two major protein bands (mol. wt. 76000 and 70000,) a minor band of mol. wt. 9600 and several minor bands with a mol. wt. of 67000 or lower are seen. Since all these components react with the monoclonal antibodies, they must have at least one antigenic determinant in common.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005959:Glucosidases / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000276:immunology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013154:Spleen / Q000276:immunology; D000520:alpha-Glucosidases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005959:Glucosidases; D000520:alpha-Glucosidases