Mutagen sensitivity, smoking habits and enzyme induction in healthy middle-aged men.
Unscheduled DNA synthesis (UDS) (excision-repair) of N-acetoxy-2-acetylaminofluorene (NA-AAF) damage to the DNA of human lymphocytes and levels of 3H-labeled NA-AAF bound to the DNA (carcinogen binding) of lymphocytes after 18 hr of culturing were measured in a consecutive subsample of healthy middle-aged males attending a multiphasic health screening program at the Department of Preventive Medicine in Malmö during 3 weeks in November-December 1981, and compared relative to their smoking habits, body weight, serum cholesterol, and gamma-glutamyltransferase levels as well as aryl hydrocarbon hydroxylase inducibility. This study group numbered 66 males and was uniform in sex, age, and investigation time. No case of significant arterial hypertension was present. The UDS and carcinogen binding results showed no correlation with the other factors measured, with the exception of smoking which was strongly (P less than 0.01) associated with increasing levels of both the UDS and carcinogen binding values. It is concluded that under ordinary circumstances smoking may represent the most important exogenous factor which may modulate risk to cardiovascular disease and cancer by influencing individual mutagen sensitivity.
D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology; D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D004247:DNA / Q000096:biosynthesis*; D004790:Enzyme Induction; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D012907:Smoking; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis*
D000099:Acetoxyacetylaminofluorene; D004247:DNA; D002784:Cholesterol; D001189:Aryl Hydrocarbon Hydroxylases; D005723:gamma-Glutamyltransferase