[Production of heterologous antibodies to T-killers of mice immunized against H-2 antigens].
Rabbit antisera were obtained against cytotoxic small peritoneal lymphocytes (IPEL) of CBA (H-2k) mice immune to alloantigens C57BL/6 (H-2b) and to the enriched 5-day MLC cytotoxic blast lymphocytes (MLC--CL). After appropriate absorption by cells and tissues of intact mice the cytotoxicity of the sera was lost relative to normal lymphoid cells. The absorbed anti-CPL serum inhibited, in the presence of complement, the cytotoxic effect of CPL but not that of MLC--CL on 51Cr-labeled allogeneic macrophages. This inhibition was restricted by idiotypic and strain specificity. Conversely, the absorbed anti-MLC--CL serum inhibited the cytotoxic effect of both CPL and MLC--CL of various mouse strains, irrespective of their immunologic specificity. It is supposed that the effect of the anti-CPL serum is mainly caused by antibodies againts idiotypic determinants of the killer T receptors, whereas the effect of the anti-MLC--CL serum is due to antibodies against differentiation antigens of the proliferating lymphocytes.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface / Q000276:immunology*; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D000939:Epitopes; D006183:H-2 Antigens / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR / Q000276:immunology; D008807:Mice, Inbred BALB C / Q000276:immunology; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA / Q000276:immunology; D013045:Species Specificity
D000954:Antigens, Surface; D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera
Poluchenie geterologichnykh antitel k T-killeram mysheĭ, immunnym k antigenam H-2.