[Translation of mouse interferon messenger RNA in a cell-free system].
Crude mouse interferon mRNA extracted from poly (I) . poly (C)-induced L929 cells has been translated in a cell-free system from rabbit reticulocytes and in two-cell systems--L929 cells and chick embryo fibroblasts. Translational efficiency of interferon mRNA preparation was 100-fold higher in the cell-free system than in tissue culture cells. Interferon synthesized was species-specific and its antiviral activity was completely neutralized by mouse interferon antiserum.
D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D005347:Fibroblasts / Q000378:metabolism; D007372:Interferons / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013045:Species Specificity
D012333:RNA, Messenger; D007372:Interferons
Transliatsiia inforrmatsionnoĭ RNK myshinogo interferona v beskletochnoĭ sisteme.