title: Recent studies on the involvement of retinyl phosphate as a carrier of mannose in biological membranes.
abstract: Rat liver microsomes synthesized [14C]mannosylretinylphosphate and dolichyl [14C]mannosylphosphate from guanosinedisphosphate [14C]mannose, retinylphosphate and dolichylphosphate. Two distinct enzyme activities were shown to be responsible for the biosynthesis of the two mannolipids. A higher affinity mannosyl transferase (EA I), responsible for dolichylmannosylphosphate synthesis, displayed a Km for GDP-mannose of 1.7 microM; while a lower affinity enzyme (EA II), responsible for mannosylretinylphosphate synthesis, displayed a Km for GDP-mannose of 12.5 microM. These Km values were unaffected by the addition of either dolichylphosphate for EA II, or retinylphosphate for EA I. The same Km values were found before and after solubilization of the enzyme activity with 1% Triton X-100. Differential solubilization of EA I and EA II was demonstrated, utilizing different concentrations of Triton X-100. Triple-labeled mannosylretinylphosphate was prepared from [3H]retinylphosphate, retinyl[32P]phosphate and GDP-[14C]mannose from incubations containing rat liver microsomes. This compound was shown to donate [14C]mannose to endogenous acceptors of rat liver microsomes.
mesh_terms: D000818:Animals; D004224:Diterpenes; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006600:Hexosephosphates / Q000096:biosynthesis*; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008360:Mannosephosphates / Q000096:biosynthesis*; D008364:Mannosyltransferases / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D051381:Rats; D014801:Vitamin A / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D004224:Diterpenes; D006600:Hexosephosphates; D008360:Mannosephosphates; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D011106:Polyisoprenyl Phosphates; D014801:Vitamin A; D006155:Guanosine Diphosphate Mannose; C012532:retinol phosphate; D004287:Dolichol Monophosphate Mannose; C013101:mannosylretinylphosphate; D006602:Hexosyltransferases; D008364:Mannosyltransferases