In vitro synthesis of alpha-fetoprotein and human chorionic gonadotropin in testicular cancer.
In vitro synthesis of alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG) utilizing 14C-L-leucine and 14C-L-lysine incorporation in fresh testicular cancer tissue of five patients was demonstrated. Radioimmunoassay, immunoprecipitation of 14C-HCG and 14C-AFP, and quantitation of AFP and HCG. This in vitro synthesis of AFP and HCG was correlated with immunocytochemical staining in the cancer cells of the five patients.
D000328:Adult; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D004407:Dysgerminoma / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D007930:Leucine / Q000494:pharmacology; D008239:Lysine / Q000494:pharmacology; D008297:Male; D011863:Radioimmunoassay; D013724:Teratoma / Q000378:metabolism; D013736:Testicular Neoplasms / Q000097:blood / Q000378:metabolism*; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis / Q000096:biosynthesis*
D006063:Chorionic Gonadotropin; D000509:alpha-Fetoproteins; D007930:Leucine; D008239:Lysine