Acridine orange-methyl green fluorescent staining of nucleoli.
The staining of nucleoli with the fluorescent dye acridine orange following by counter-staining with methyl green differentially stained nucleoli in both plant and animal cells. The nucleoli fluoresced as bright structures highlighted against the quenched fluorescence of the chromatin. This technique provides a simple and highly reproducible method for differential staining of nucleoli.
D000165:Acridine Orange; D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D005453:Fluorescence; D006801:Humans; D008739:Methyl Green; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D012394:Rosaniline Dyes; D008739:Methyl Green; D000165:Acridine Orange