[Adsorption of plasma proteins on the surface of hydrophobic polymers. Structural aspects].
The qualitative and quantitative composition of the adsorbed protein layer is determined by thromboresistance of artificial polymer surface. The adsorption of albumine and gammaglobuline from buffer solution to the hydrofobic polymer surfaces was investigated with ESR and electron spectroscopic method. The existence of two kinds of protein covering was demonstrated: reversible and irreversible protein layer. During the nitroxyl labelled globuline adsorption the spectrum of label is due to superposition of both reversible and irreversible kinds of adsorption. It was demonstrated by the electron microscopic method that the strongly adsorbed protein layer corresponds with the denaturated protein, the reversibly adsorbed protein in contrast is native. The architecture of protein layer on polymer surface was also investigated. The comparison of structural and physiological properties of serum protein is important for the investigation of the thromboresistant materials.
D000327:Adsorption; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D011095:Polyethylenes / Q000494:pharmacology*; D011487:Protein Conformation; D012709:Serum Albumin / Q000032:analysis*; D013057:Spectrum Analysis; D013499:Surface Properties; D005719:gamma-Globulins / Q000032:analysis*
D011095:Polyethylenes; D012709:Serum Albumin; D005719:gamma-Globulins
Adsorbtsiia plazmennykh belkov na poverkhnosti gidrofobnykh polimerov. Strukturnyĭ aspekt.