[Spectrophotometric study of the surface of human erythrocytes and their ghosts by using a phthalocyanine dye].
Human intact erythrocytes and their ghosts (resealed and unresealed) were stained by the vital dye Heliogen Blue, which does not penetrate the cell being only sorbed by its membrane. The absorption spectra of the sorbed dye were examined. On the external surface of the membrane, the molecular (monomer) state of dye sorption is revealed, whereas on the interior side the dye sorption is mostly in the polymolecular (dimer) state. The differences in staining can be evaluated by the monomer-dimer ratio (M/D), which may be used for comparison between the sorption characteristics of membrane preparations.
D000327:Adsorption; D003300:Copper / Q000032:analysis; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D007211:Indoles / Q000032:analysis; D008665:Metalloporphyrins / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013499:Surface Properties
D007211:Indoles; D008665:Metalloporphyrins; C007911:heliogen blue SBL; D003300:Copper
Spektrofotometricheskoe issledovanie poverkhnosti éritrotsi ov cheloveka i ikh teneĭ s pomoshch'iu ftalotsianinovogo krasitelia.