Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red S.
The procedure described by Inouye ('76) for the staining of full-term mouse fetal skeletons has been adapted for use with mouse embryos and fetuses of days 14-18 of gestation. The main adaptations for younger specimens involve a longer time in acetone, in lieu of skinning, and omission of the aqueous KOH step. These adaptations require more time but result in consistently good staining of intact specimens.
D000423:Alcian Blue; D000818:Animals; D000880:Anthraquinones; D001842:Bone and Bones / Q000033:anatomy & histology*; D002356:Cartilage / Q000033:anatomy & histology*; D005260:Female; D005333:Fetus / Q000033:anatomy & histology*; D005865:Gestational Age; D006652:Histological Techniques; D051379:Mice; D011247:Pregnancy; D013194:Staining and Labeling
D000880:Anthraquinones; C004468:Alizarin Red S; D000423:Alcian Blue