Radiochemical cross-linking between proteins and RNA within 70 S ribosomal particles from E. coli MRE600.
In the absence of oxygen, gamma-irradiation produces covalent links between some ribosomal proteins and 16 S RNA to 23 S RNA, within 70 S ribosomes from E. coli MRE600. Under optimal conditions minimizing the structural modifications induced by radiations, in situ formed cross-links appear specific and reflect close RNA-protein contacts. In view of these results, the spatial organization of the 30 S, 50 S subunit interfaces is discussed. In addition, the gamma-irradiation technique reveals that subunit association induces modifications of some protein--RNA interactions.
D001426:Bacterial Proteins / Q000378:metabolism / Q000528:radiation effects*; D002499:Centrifugation, Density Gradient; D004586:Electrophoresis; D004926:Escherichia coli / Q000378:metabolism*; D005374:Filtration; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000378:metabolism / Q000528:radiation effects*; D012335:RNA, Ribosomal / Q000378:metabolism / Q000528:radiation effects*; D012269:Ribosomal Proteins / Q000378:metabolism / Q000528:radiation effects*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins