Dopamine and depolarizing agents regulate the state of phosphorylation of protein I in the mammalian superior cervical sympathetic ganglion.
The regulation of the state of phosphorylation of protein I, a specific neuronal protein that appears to be associated predominantly with synaptic vesicles, has been studied in intact sections of bovine superior cervical ganglion. For this purpose, a technique was developed that made possible the quantitation of the state of phosphorylation of as little as 5 fmol of protein I. Incubation of ganglion sections in the presence of dopamine, 8-bromo-cyclic AMP, or depolarizing agents (i.e., high K+ concentration or veratridine) increased the state of phosphorylation of protein I relative to that of control ganglion sections. Other results indicated that the effect of dopamine is probably mediated via the activation of a cyclic AMP-dependent protein kinase and that the effect of high K+ concentration is probably mediated via the activation of a calcium-dependent protein kinase.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D004298:Dopamine / Q000494:pharmacology*; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D014701:Veratridine; D011188:Potassium; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine; D004298:Dopamine; D010646:Phentolamine