title: In vitro effects of aflatoxin B1 on the activities of three hydrolases.
abstract: The in vitro effects of aflatoxin B1 on the activities of trypsin, amylase and lipase in the pancreatic extract of male albino Wistar rats have been studied. Activities of the 3 hydrolases were inhibited at 1 microgram/ml, 200 microgram/ml and 400 microgram/ml concentrations of aflatoxin B1, with the exception that at the 1 microgram/ml concentration the activity of lipase was stimulated. Lineweaver-Burk reciprocal plots indicate non-competitive inhibition of the 3 hydrolases.
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000681:Amylases / Q000037:antagonists & inhibitors; D000818:Animals; D006867:Hydrolases / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008049:Lipase / Q000037:antagonists & inhibitors; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D000348:Aflatoxins; D014361:Trypsin Inhibitors; D016604:Aflatoxin B1; D006867:Hydrolases; D008049:Lipase; D000681:Amylases