title: Receptor-mediated endocytosis in cultured fibroblasts: cryptic coated pits and the formation of receptosomes.
abstract: Concentrative receptor-mediated endocytosis of many specific ligands by cultured fibroblasts occurs through the coated pit-receptosome pathway. The formation of receptosomes was studied using two impermeant electron-dense labels for the cell surface, ruthenium red and concanavalin A-horseradish peroxidase. These studies show that at 4 degrees C, virtually all coated structures near the plasma membrane are in communication with the cell surface, and are not isolated coated vesicles. On warming cells to 37 degrees C for only 1 minute, a major portion of these structures become cryptic, that is, not labeled by these surface markers. However, on cooling cells immediately back to 4 degrees C, virtually all of these structures are again in communication with the surface. Many images showed that membrane of these cryptic pits to be continuous with the cell surface when caught in the appropriate plane of section; often there was a very narrow entrance that excluded extracellular label. At 37 degrees C, receptosomes could be occasionally seen forming as an invagination of membrane adjacent to the coated region. Mechanisms by which receptosomes may form and other evidence demonstrating the failure of coated pits to pinch off to form isolated coated vesicles during endocytosis are discussed.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D004705:Endocytosis; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D051379:Mice; D008854:Microscopy, Electron; D009940:Organoids / Q000648:ultrastructure*; D012430:Ruthenium Red
chemical_list: D003208:Concanavalin A; D012430:Ruthenium Red; D006735:Horseradish Peroxidase