title: Comparative study of lipogenic enzymes in several vertebrates.
abstract: The liver lipogenic enzymes are compared among rats, chickens, frogs and fish. Although the apparent Km values of glucose-6-phosphate dehydrogenase for glucose-6-phosphate are not much different among all the species, those of malic enzyme for malate are much higher in chickens and fish than in rats and frogs. Glucose-6-phosphate dehydrogenase showed very high activities compared with malic enzyme in fish liver, and malic enzyme showed high activities in chicken liver. Although the apparent Km values of acetyl-CoA carboxylase and fatty acid synthetase for substrates are in the same range among all the animals, the activity of acetyl-CoA carboxylase seems to be extremely low in fish and frog livers, and that of fatty acid synthetase is low in frog livers only. In addition, the apparent Km values of alpha-glycerophosphate acyltransferase of fish liver are very high, and the enzyme activity appears to be extremely low compared to the others. Therefore, the enzymes at the first steps of both fatty acid and glycerolipid syntheses of poikilothermos animals appear to be very low. On the other hand, the Ouchterlony double-diffusion patterns showed that the lipogenic enzymes of chickens, frogs and fish are immunologically different from those of rats, with the exception of acetyl-CoA carboxylase in chickens. Therefore, it is suggested that the fatty acid and glycerolipid forming systems of poikilothermos animals are quite different from those of homoiothermos and the lipogenesis is very low in poikilothermos.
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002025:Bufonidae; D002645:Chickens; D051048:Diacylglycerol O-Acyltransferase; D004032:Diet; D064429:Fatty Acid Synthases / Q000378:metabolism; D005399:Fishes; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D005779:Immunodiffusion; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000085:Acetates; D008055:Lipids; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D051048:Diacylglycerol O-Acyltransferase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase; D019342:Acetic Acid