Purification and properties of autolytic endo-beta-N-acetylglucosaminidase and the N-acetylmuramyl-L-alanine amidase from Bacillus subtilis strain 168.
beta-N-Acetylglucosaminidase has been purified from the walls of Bacillus subtilis 168 and compared with the other known autolysin, N-acetylmuramyl-L-alanine amidase (amidase). The beta-N-acetylglucosaminidase was a dimer in LiCl buffers with a sub-unit molecular weight of 90000 and a pH optimum of about 5.0. It was very sensitive to proteolytic enzymes and was critically activated by 0.1 to 0.2 M-LiCl. It was insoluble in concentrations of LiCl lower than 0.05 to 0.1 M. It was less strongly bound to walls than was the amidase, which was a monomer of molecular weight 30000 to 40000 in LiCl buffers. The beta-N-acetylglucosaminidase is an endo-enzyme and showed no exo-activity. Lysozyme-like enzyme (muramidase) activity was undetectable in the wall extracts examined.
D000118:Acetylglucosaminidase / Q000302:isolation & purification* / Q000378:metabolism; D000581:Amidohydrolases / Q000302:isolation & purification*; D001412:Bacillus subtilis / Q000201:enzymology*; D002473:Cell Wall / Q000201:enzymology; D006596:Hexosaminidases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D008070:Lipopolysaccharides; D008094:Lithium / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000302:isolation & purification* / Q000378:metabolism; D010712:Phosphatidic Acids / Q000494:pharmacology; D013379:Substrate Specificity; D013682:Teichoic Acids / Q000494:pharmacology
D008070:Lipopolysaccharides; D010712:Phosphatidic Acids; D013682:Teichoic Acids; C009900:lipoteichoic acid; D008094:Lithium; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000581:Amidohydrolases; D009238:N-Acetylmuramoyl-L-alanine Amidase; D008274:Magnesium