Collodion membrane secures cells sorted by flow cytofluorometry onto microscope slides.
Quantitative microscopic cytology of cells previously sorted by flow cytofluorometry has been hindered by the loss of cells from the microscope slide during staining procedures. The simple application of a semi-permeable membrane of collodion over fixed or unfixed cells sorted directly onto a microscope slide secured virtually 100% of the cells onto the slide. Cells covered with the collodion membrane studied with Papanicolaou's stain as well as routine clinical cervical cytologic preparations. In contrast, fewer than one half of the cells sorted onto uncoated or albumin coated slides were retained after staining.
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002469:Cell Separation / Q000379:methods*; D002610:Cheek; D003101:Collodion; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008853:Microscopy; D009061:Mouth Mucosa / Q000166:cytology; D013194:Staining and Labeling
D003101:Collodion