Studies of marmoset megakaryocytopoiesis in vitro.
Liquid culture of the mononuclear fraction of the blood of marmosets without any added stimulator resulted in the appearance of megakaryocytes. These cells could not be identified by light microscopy or by indirect immunofluorescence using antiplatelet antiserum. Ultrastructural identification was made using platelet peroxidase. Some megakaryocytes demonstrated sparse but detectable demarcation membranes, early granule formation, and platelet formation. The results indicate that marmoset megakaryocytic progenitor cells circulate and can begin to differentiate in vitro, although unstimulated liquid culture conditions do not promote the development of the usual light-microscopic features of differentiation.
D000818:Animals; D001792:Blood Platelets / Q000276:immunology / Q000648:ultrastructure; D002143:Callitrichinae; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006410:Hematopoiesis; D008264:Macrophages / Q000648:ultrastructure; D008533:Megakaryocytes / Q000276:immunology; D010544:Peroxidases / Q000378:metabolism; D013194:Staining and Labeling
D010544:Peroxidases