Sequence diversity of nuclear and polysomal polyadenylated and non-polyadenylated RNA in normal and regenerating rat liver.
A DNA probe purified from RNA . DNA hybrids of total sham-operated liver RNA and non-repetitive DNA was used to show that nuclear poly(A)-rich RNA from sham-operated liver and from 2.5-h and 48-h regenerating liver contains about 50% of the complexity of total liver RNA. It was further shown that the differences between normal and regenerating liver, reported earlier from this laboratory, occur in the poly(A)-free fraction of nuclear RNA. At the polysome level it was found that polysomal RNA has one-third of the sequence diversity of total RNA and of this, approximately 65% can be accounted for by poly(A)-rich and 55% by poly(A)-free molecules. When DNA probes were prepared from hybrids formed using polysomal RNA from sham-operated liver and regenerating liver at 2.5 h and 48 h post-hepatectomy and then employed in reactions with homologous and heterologous RNAs, no differences were detectable between either normal and regenerating liver or regenerating liver at times during hypertrophy and hyperplasia.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA