Dimethylsulfoxide-induced alterations in the growth properties and protein composition of in vitro-propagated murine hepatoma cells.
The effects of the differentiation-inducing agent dimethylsulfoxide (DMSO) on the growth properties and protein synthetic capacity of BW77-1 mouse hepatoma cells were compared at various media concentrations of the polar solvent. DMSO produced a dose-dependent reduction in final population density, reduced or eliminated cell piling and stimulated synthesis of albumin. The rising albumin content reflected dose-related DMSO-induced increases in total cellular protein and in the albumin contribution to total cellular protein. In order to determine whether viral gene expression was associated with DMSO-induced stimulation of albumin synthesis, BW77-1 cultures were examined for the production of ecotropic and xenotropic type C virus. The BW77-1 hepatic tumor cell line was determined to be a nonproducer of type C virus by assays designed to measure extracellular reverse transcriptase, viral env gene product and infectivity on mouse and mink indicator cells. Type C virus could not be induced in BW77-1 cultures by treatment with DMSO under conditions which lead to a reduced proliferative capacity and enhanced expression liver-specific genes.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000302:isolation & purification
D000418:Albumins; D009363:Neoplasm Proteins; D012194:RNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide