A modified procedure for the determination of 5-hydroxyindoleacetic acid in the urine and cerebrospinal fluid of patients with hepatic cirrhosis.
A method has been developed for the separation of 5-hydroxyindoleacetic acid from substances in urine and cerebrospinal fluid which interfere with its fluorometric determination. This involves the use of anion exchange column chromatography with elution of the 5-hydroxyindoleacetic acid by 3 mol/l formic acid in 60% methanol. The indole acid is then assayed fluorometrically after reaction with o-phtalaldehyde. Urinary 5-hydroxyindoleacetic acid values are reported for patients with stable cirrhosis and hepatic encephalopathy and compared with those found in control subjects without liver disease.
D002852:Chromatography, Ion Exchange; D003404:Creatinine / Q000652:urine; D005470:Fluorometry; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine; D008103:Liver Cirrhosis / Q000378:metabolism*
D006897:Hydroxyindoleacetic Acid; D003404:Creatinine