[Characteristics of the distribution in a 2-phase polymer system of L cells at different stages of culture growth].
By the method of counter-current distribution in two-phase polymer system Dextran-500/poly (ethylene)glycol-6000, surface properties of monolayer and suspension sublines of L cells were investigated in relation to the stage of culture growth. The cultivation conditions of both the cell types were identical. It has been shown that with the growth of cell population, the number of cells with lower partition coefficient increases in both the sublines, mostly in cells of monolayer subline. These data suggest more essential changes of surface properties of cells growing in monolayer. It is supposed that this can be associated with the inability of suspension cells to form adhesion contacts.
D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D003377:Countercurrent Distribution; D003911:Dextrans / Q000494:pharmacology; D007739:L Cells / Q000502:physiology*; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology; D013499:Surface Properties; D013535:Suspensions
D003911:Dextrans; D013535:Suspensions; D011092:Polyethylene Glycols
Osobennosti raspredeleniia v dvukhfaznoi polimernoi sisteme L-kletok, nakhodiashchikhsia na raznykh stadiiakh rosta kul'tury.