Immunoelectronmicroscopy of metaphase chromosomes.
A method for the preparation of ultrathin sections of metaphase chromosomes is described. This method was applied to human metaphase chromosomes, which were immunocytochemically stained with anti-DNA and anti-ribonucleoprotein antibodies, derived from patients with auto-immune disease. Conventionally prepared metaphase spreads as well as cytocentrifuge preparations of chromosome suspensions were studied. The results indicate that the ultrastructure of chromosomes and the immunoreactivity of chromosomal constituents are influenced by the applied preparation methods. In comparison with whole mount preparations, ultrathin sections of immunostained chromosomes allow higher resolution and more precise localization of immunoreactive sites within the chromosomal structure.
D000974:Antibodies, Antinuclear; D000937:Antigen-Antibody Reactions; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D008677:Metaphase; D008854:Microscopy, Electron; D013194:Staining and Labeling
D000974:Antibodies, Antinuclear