[Isolation and identification of antigen-specific T-cell-receptors (author's transl)].
Experimental allergic encephalomyelitis was induced by injection of myelin basic protein into guinea pigs strain 13. Specific antigen binding T-cell receptors were isolated by means of a nylon net coupled with antigen. A phage immunoassay was set up as an analytical tool for detection of the receptors. They were found not to be identical with common immunoglobulins and have a molecular weight of 71,000.
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology*; D000939:Epitopes; D006168:Guinea Pigs; D007118:Immunoassay / Q000379:methods; D007136:Immunoglobulins / Q000032:analysis; D008970:Molecular Weight; D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000302:isolation & purification; D013604:T-Phages
D000939:Epitopes; D007136:Immunoglobulins; D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell
Isolierung und Nachweis antigenspezifischer T-Zell-Rezeptoren.