Protein synthesis in chloroplasts. VIII. Differential synthesis of chloroplast proteins during spinach leaf development.
Excised primary leaves of spinach (Spinacia oleracea) incorporate [35S]-methionine into a number of chloroplast polypeptides. The ratio of incorporation of isotope into the large subunit of ribulose bisphosphate carboxylase relative to a thylakoid polypeptide (peak D) decreases during leaf development in whole leaves; this changing pattern of incorporation is also observed in isolated chloroplasts where these two polypeptides are the major products of protein synthesis. Chloroplast RNA prepared from developing leaves was translated in a reticulocyte lysate extract to yield full-length carboxylase large subunit and peak D polypeptides. The fidelity of translation of these two polypeptides was checked by partial protease digestion. Changes in the synthesis of the large subunit of the carboxylase and peak D in developing leaves are reflected in changes in the amount of translatable mRNA for these two polypeptides.
D000818:Animals; D001345:Autoradiography; D002736:Chloroplasts / Q000378:metabolism*; D010455:Peptides / Q000032:analysis; D063245:Plant Development; D010940:Plant Proteins / Q000096:biosynthesis*; D010944:Plants / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D010455:Peptides; D010940:Plant Proteins; D012333:RNA, Messenger; D012313:RNA