Inhibition of collagen accumulation by glucocorticoids in rat lung after intratracheal bleomycin instillation.
Male Fischer 344 rats were given a single lung instillation of bleomycin sulfate (0.6 units/100 g). some animals were treated 24 hr after bleomycin administration with triamcinolone diacetate. Steroid treatment was continued on alternate days for 4 weeks. At the end of 4 weeks, the lungs of rats receiving bleomycin alone had two-fold increases of both prolyl hydroxylase activity and proteinaceous hydroxyproline as compared to control values. The lungs of bleomycin-treated rats which received 4 mg of triamcinolone diacetate per kg on alternate days had a 33% increase of prolyl hydroxylase activity and proteinaceous hydroxyproline content of bleomycin-treated animals receiving glucocorticoid (8 mg/kg) on alternate days were the same as control values. The results indicate that alternate day administration of the synthetic glucocorticoid triamcinolone diacetate blocks lung collagen accumulation following a single intratracheal dose of bleomycin to rats.
D000818:Animals; D001761:Bleomycin / Q000009:adverse effects*; D003094:Collagen / Q000096:biosynthesis*; D006909:Hydroxyproline / Q000032:analysis; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D011393:Procollagen-Proline Dioxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D014221:Triamcinolone / Q000494:pharmacology*
D001761:Bleomycin; D014221:Triamcinolone; D003094:Collagen; D011393:Procollagen-Proline Dioxygenase; D006909:Hydroxyproline