Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles.
Apolipoprotein AI of rats has been synthesized in a cell-free wheat germ system and cotranslationally translocated into dog pancreas microsomal vesicles. Translocation is accompanied by cleavage of a signal sequence of 18 amino acid residues and is dependent on the recently purified signal recognition protein.
D000595:Amino Acid Sequence; D000818:Animals; D016633:Apolipoprotein A-II; D001053:Apolipoproteins / Q000378:metabolism*; D001692:Biological Transport; D002474:Cell-Free System; D004285:Dogs; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D014908:Triticum / Q000378:metabolism
D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D012313:RNA