title: Automated two-site immunoradiometric assay of human alpha-fetoprotein in maternal serum.
abstract: Immunoassays of alpha 1-fetoprotein in maternal serum are now in widespread use as a means of antenatal detection of neural tube defects. For population screening to be effective the initial test must be capable of promptly producing reliable results. We describe an automated two-site immunoradiometric assay based on 125I-labeled antibodies and cellulose-coupled antibody, with which up to 100 patients' samples can be assayed in 3 h. The assay is precise, and results obtained agree closely with those produced by conventional radioimmunoassay procedures.
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011863:Radioimmunoassay / Q000379:methods; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D007074:Immunoglobulin G; D000509:alpha-Fetoproteins