V and C genes in creating antibodies directed to alpha (1 replaced by 3)-linked D-glucosyls in dextran. III. Frequency of alpha (1 replaced by 3)-responsive, primary B precursors in a1 high- and b low-receptor allogroups.
An evaluation was made of the frequency of alpha (1 replaced by 3)-responsive, primary B precursors and their reactivity profiles in Igh-1al (al) and Igh-1b (beta) allogroup mice, of dissimilar responsiveness as to quantity of the specific serum antibody produced, its light chain isotype, and its variable-region, ligand-modifiable antigenicity, to alpha (1 replaced by 3) glucosyls in dextran B-1355. al mice, exhibited highly alpha 1-specific serum antibody, with relatedness to the idiotypes (Id) shared by three BALB/c, alpha (1 replaced by 3)-specific, gamma 1 myeloma proteins, possess specific primary splenic B precursors at a frequency of 1/5.5-7.3 x 10(4) cells; beta mice, exhibiting low, k serum, Id- antibody, possess B splenic-specific primary precursors at a frequency of 1/1.0-1.4 x 10(5) cells. A radioimmunoassay analysis showed correlations between the isotype profiles of the serum antibody response and of the primary precursors, namely mu gamma 1 in the al and mu kappa in the beta allogroups, but also delineated minor primary mu gamma lambda 1 and mu kappa precursors in al and mu gamma kappa and mu gamma 1 in b, at frequencies of 1/3 x 10(5) to 1/6.5 x 10(6). The al, gamma 1 precursors exhibited defined reactivity patterns for 3-mono-O-3,6-di-O-glucosyls and non-reducing end groups in the B-1355. The beta, kappa precursors recognized the sequential residues.
D000818:Animals; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001666:Binding Sites, Antibody / Q000235:genetics*; D002454:Cell Differentiation; D003911:Dextrans / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005947:Glucose / Q000276:immunology*; D007127:Immunoglobulin Constant Regions / Q000096:biosynthesis / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D007164:Immunosorbents / Q000494:pharmacology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009194:Myeloma Proteins / Q000032:analysis / Q000276:immunology; D011863:Radioimmunoassay; D013154:Spleen / Q000276:immunology
D003911:Dextrans; D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D007164:Immunosorbents; D009194:Myeloma Proteins; D005947:Glucose