Aetiology of jaagsiekte: transmission by means of subcellular fractions and evidence for the involvement of a retrovirus.
Jaagsiekte (ovine pulmonary adenomatosis) was transmitted to new-born lambs by inoculation of the microsomal fraction of a cytoplasmic extract of cultured tumour cells or tumour tissue. Various treatments of the biologically active fraction were carried out to differentiate between various classes of possible aetiological agents. The results obtained suggested the involvement of a membrane-associated RNA containing virus. Reverse transcriptase activity dependent on Mg++ was subsequently demonstrated in these extracts and in lung exudate, and was shown to be associated with particles banding at a density 1,175 in sucrose gradients. These characteristics, as well as the appearance of the particles in the electron microscope, are similar to those reported for Type B and Type D retroviruses. Serial transmissions of jaagsiekte over a number of years, using cytoplasmic extracts and purified virus, strongly suggest that this virus is the aetiologic agent of jaagsiekte.
D000818:Animals; D002458:Cell Fractionation; D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification; D008168:Lung / Q000382:microbiology / Q000648:ultrastructure; D011648:Pulmonary Adenomatosis, Ovine / Q000209:etiology / Q000382:microbiology / Q000635:transmission*; D012367:RNA, Viral / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification*; D012756:Sheep; D013347:Subcellular Fractions
D004279:DNA, Viral; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase