A rapid centrifugation method for the isolation of polymorphonuclear leucocytes from human blood.
A rapid one-step gradient centrifugation method to prepare PMN leucocytes has been worked out by which a 98--99% pure, washed granulocyte suspension of 98% viability can be obtained in 30--40 minutes. The cells prepared by this method displayed higher NBT reduction upon ingesting the same soluble DNA-anti DNA complexes than those prepared by the dextran sedimentation method.
D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D003911:Dextrans; D006801:Humans; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology; D010587:Phagocytosis; D013997:Time Factors
D003911:Dextrans