Differential pulse voltammetry in brain tissue. I. Detection of 5-hydroxyindoles in the rat striatum.
In vitro, differential pulse voltammetry combined with electrochemically treated carbon fiber electrodes enabled detection, in different solution of 5-hydroxyindole compounds, of an oxidation peak 3 at +300 mV. In vivo, a striatal peak 3 was also recorded at this potential. Electrolytic or 5,7-dihydroxytryptamine lesions interrupting the medial forebrain bundle (MFB) were followed by a decrease of 65% and 64% in peak height, but not elimination of the peak. Biochemical determinations were significantly correlated to the peak 3 measurements. The existence of peak 3 as well as hydroxyindole compounds in blood suggested a blood contamination under the experimental conditions employed. This possibility is confirmed both by the complete disappearance of striatal peak 3 in animals with the MFB lesioned and surgically prepared a week before recordings, and by biochemical measurements in parachlorophenylalanine-treated or perfused (phosphate-buffered saline solution) animals.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000494:pharmacology; D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology; D006916:5-Hydroxytryptophan / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D003342:Corpus Striatum / Q000032:analysis* / Q000187:drug effects; D004558:Electric Stimulation; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D008297:Male; D011199:Potentiometry / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*
D015102:3,4-Dihydroxyphenylacetic Acid; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006916:5-Hydroxytryptophan; D001205:Ascorbic Acid