Correlation between LPS-induced mitogenic and virogenic activities in mouse spleen cell cultures.
The present study provides direct evidence for a correlation between mitogenicity and induction of type C particles (virogenic activity) in cultures stimulated by LPS. This evidence is based on the following observations: 1) The complex of the antibiotic polymyxin B with LPS (LPS-PB) inhibits mitogenicity and virogenic activity in spleen cell cultures from BALB/c mice, but does not inhibit the generation of colony stimulating factor (CSF) from such cultures; 2) CBA/N, a genetically-defective mouse strain in its mitogenic response to LPS, was also defective in virus induction by LPS, but generated high levels of LPS-induced CSF.
D000818:Animals; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011112:Polymyxin B / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae; D013154:Spleen / Q000166:cytology*; D013997:Time Factors
D003115:Colony-Stimulating Factors; D008070:Lipopolysaccharides; D012194:RNA-Directed DNA Polymerase; D011112:Polymyxin B