Factors affecting the assay of bovine type I interferon on bovine embryonic lung cells.
One preparation of interferon (IF) on 7-day-old bovine embryonic lung cultures free of bovine viral diarrhea virus had titers ranging from 20 to 10,240 in plaque-reduction tests, using bovine vesicular stomatitis virus. Factors believed to contribute to this variation were investigated. Although titers differed on different cell strains, different passages, and on cultures of different ages, variations between the two assays definitely could not be established as a function of these factors. However, IF titers on low passage cultures were usually lower than were titers on subsequent passages of the same cell strain. Calf serum in the diluent for IF reduced the titer one or two twofold dilutions. Over the range of 7 to 24 hours contact of IF with bovine embryonic lung cultures, and titer decreased steadily and was one twofold dilution less at 24 hours than it was at 7 hours. Latent viruses were not found in cultures by electron microscopy. Treatment of cultures with a noncytopathogenic strain of bovine viral diarrhea virus almost eliminated the effect of IF. Naturally occurring production of IF was not a major problem. While IF was on the cells, the pH of IF had a pronounced effect on the titer and may have been responsible for the vatiation observed with different passages, different cell strains, use of cultures of different ages, and use of calf serum in the diluent. Interferon at a low pH had a titer three or four twofold dilutions less than did the IF at a high pH.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007372:Interferons / Q000032:analysis*; D008168:Lung; D013997:Time Factors; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D010948:Viral Plaque Assay
D003470:Culture Media; D007106:Immune Sera; D007372:Interferons