title: Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse.
abstract: To compare the structure of IgG2a alleles we have determined the complete DNA sequence of the constant region, coding sequence, and 3' untranslated region of a cDNA clone, pAB gamma 2a-1, which was derived from the C57BL/6 mouse strain (b allotype). This sequence was compared with the corresponding IgG2a DNA sequence of BALB/c origin (a allotype). The DNA sequences showed 10% differences, and the deduced protein sequences differed by about 15%. These differences were not evenly distributed: most differences were in the hinge region, the CH3 domain and the 3' untranslated region. It is evident that many alterations in the IgG2a alleles have occurred since the a and b haplotypes were separated--some of these changes were point mutations but some appear to have resulted from gene conversion of the IgG2ab allele by the IgG2bb allele.
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004274:DNA, Recombinant; D000939:Epitopes; D005785:Gene Conversion; D005796:Genes; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007142:Immunoglobulin gamma-Chains / Q000235:genetics*; D051379:Mice; D010957:Plasmids
chemical_list: D004274:DNA, Recombinant; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains