Solid-phase radioimmunoassay for detection of alcoholic hyalin antigen (AHAg) and antibody (anti-AH).
Alcoholic hyalin (AH) was isolated and purified from post-mortem livers of patients with alcoholic hepatitis. Antibodies against AH (anti-AH) were raised in guinea-pigs. Their specificity was demonstrated by immunofluorescence and by immunoabsorption. The antisera were positive in immunofluorescence and complement fixation up to serum dilutions of 1:320 and 1:32 respectively. In order to achieve a higher sensitivity a solid-phase radioimmunoassay (SP-RIA) was established for detection of alcoholic hyalin antigen (AHAg) and anti-AH. Anti-AH could thus be measured up to a 10(5) serum dilution. Using a blocking test, solubilized AH at protein concentrations as low as 80 to 160 ng/ml could be detected. With this SP-RIA, neither AHAg nor anti-AH was found in the sera of 32 patients with histologically proven alcoholic hepatitis. The sera were likewise negative by indirect immunofluorescence, complement fixation and immune adherence haemagglutination test.
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D003168:Complement Fixation Tests; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006519:Hepatitis, Alcoholic / Q000276:immunology*; D006801:Humans; D006818:Hyalin / Q000276:immunology*; D007104:Immune Adherence Reaction; D011863:Radioimmunoassay
D000906:Antibodies; D000941:Antigens