Ultrastructural localization of calcitonin, somatostatin and serotonin in parafollicular cells of rat thyroid.
Three hormones were demonstrated in ultrathin sections of the rat thyroid using immunocytochemical methods with either a PAP complex or a protein A-gold complex as the label. In control rats, calcitonin was found to be present in all parafollicular cells and somatostatin in occasional cells. In rats pretreated with 5-hydroxytryptophan, serotonin was detected in all parafollicular cells as well. In serial ultrathin sections, the three hormones were seen to be localized in the same secretory granules.
D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D012701:Serotonin; D013004:Somatostatin; D002116:Calcitonin; D006916:5-Hydroxytryptophan