Stimulation of cytolytic T cells by isolated viral peptides and HN protein coupled to agarose beads.
Sendai virus-infected mouse cells can be lysed by mouse cytolytic thymus-dependent lymphocytes (CTL) directed specifically against the infected cells. The CTL is known to recognise the H-2 antigens on the target cells together with structure(s) including at least the two viral surface glycoproteins also found on purified virus. We report here that anti-Sendai CTL can be stimulated in vitro by detergent-solubilised viral haemagglutinin-neuraminidase (HN), either as the isolated protein or coupled to agarose beads. We further show stimulation by the hydrophilic portion of a protein removed from the virus by the protease subtilisin BPN', and we demonstrate that cyanogen bromide- (CNBr-) cleaved viral peptides also produce such stimulation.
D000818:Animals; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004800:Enzymes, Immobilized / Q000276:immunology; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000276:immunology; D051379:Mice; D009439:Neuraminidase / Q000276:immunology; D009698:Nucleoproteins / Q000276:immunology; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D004800:Enzymes, Immobilized; D000939:Epitopes; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D009698:Nucleoproteins; D010446:Peptide Fragments; D014764:Viral Proteins; D009439:Neuraminidase