[Chromatin fractionation according to the strength of its matrix bond].
Extraction in low salt concentration followed by centrifugation allows rat liver nuclear chromatin to be divided into two fractions: the supernatant chromatin and matrix chromatin. The former fraction contains about 60-70% of initial DNA and about 15% of initial protein along with all five histones, and an insignificant amount of non-histone proteins. RNA synthesis in the matrix chromatin fraction is 2-3 times more intense than that in the original nuclei. The data on gradient centrifugation do not suggest the elongation of RNA molecules synthesized in the matrix fraction. The results obtained as compared with the literature data suggest that the matrix chromatin fraction is enriched with active genes.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis; D011485:Protein Binding; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D014461:Ultracentrifugation / Q000379:methods
D002843:Chromatin; D012313:RNA
Fraktsionirovanie khromatina po prochnosti ego sviazi s matriksom.