Establishment and characterization of a continuous lung squamous cell carcinoma cell line (U-1752).
A continuous cell line, U-1752, was established from a lung tumor originally diagnosed as a small cell carcinoma. The cell line has been in continuous in vitro passage for 29 months. The epithelial, rather than small cell nature of the U-1752 cells was demonstrated by the presence of desmosomes, prominent tonofilament bundles, by the reactivity with an anti-keratin antiserum and by the expression of cell surface receptors for epidermal growth factor (EGF). The U-1752 cells grow as monolayer cultures and have a population doubling time of around 36 hours at optimal growth in 20% calf serum. The most important neoplastic features of U-1752 were its aneuploidy, its capacity for colony formation in agarose and its tumorigenic potential subcutaneously in nude mice.
D000328:Adult; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002460:Cell Line; D002875:Chromosomes; D006728:Hormones / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007633:Keratins / Q000276:immunology; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation
D006728:Hormones; D007106:Immune Sera; D007633:Keratins