Synthesis of methotrexate polyglutamates by bone marrow cells from patients with leukemia and lymphoma.
Bone marrow cells from children with lymphoblastic leukemia, myeloblastic leukemia and non-Hodgkin's lymphoma were incubated with tritium-labelled methotrexate in short-term cultures. Cells obtained both during disease activity as well as during remission synthesized large quantities of methotrexate polyglutamates. Synthesis was time- and dose-dependent and occurred with concentrations of methotrexate regularly achieved during conventional treatment with this drug.
D001853:Bone Marrow / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism*; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D010452:Peptide Biosynthesis; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis*; D013997:Time Factors
D011099:Polyglutamic Acid; C014085:methotrexate polyglutamate; D008727:Methotrexate