title: Simple high-performance liquid chromatographic method for the concurrent determination of the amine metabolites vanillylmandelic acid, 3-methoxy-4-hydroxyphenylglycol, 5-hydroxyindoleacetic acid, dihydroxyphenylacetic acid and homovanillic acid in urine using electrochemical detection.
abstract: A simple method for the concurrent analysis of the noradrenaline metabolites vanillylmandelic acid and 3-methoxy-4-hydroxyphenylglycol, the dopamine metabolites dihydroxyphenylacetic acid and homovanillic acid, and the serotonin metabolite 5-hydroxyindoleacetic acid in human urine is described. Following organic extraction of the metabolites from acidified urine, they are separated by single-step gradient elution high-performance liquid chromatography on a reversed-phase column. Detection and quantification are achieved with an electrochemical detector using a carbon-paste electrode; samples can be injected at 40-min intervals. Optimisation of analytical parameters is described, and examples of the application of the method in the fields of clinical chemistry and clinical neuroscience are given. This provides a convenient method for the concurrent study of the metabolism of three major biogenic amines, and is readily adaptable for studies on cerebrospinal fluid and brain tissue.
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000652:urine*; D000310:Adrenal Gland Neoplasms / Q000652:urine; D002230:Carbidopa / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006018:Glycols / Q000652:urine*; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D008734:Methoxyhydroxyphenylglycol / Q000652:urine*; D010648:Phenylacetates / Q000652:urine*; D010673:Pheochromocytoma / Q000652:urine; D014642:Vanilmandelic Acid / Q000652:urine*
chemical_list: D006018:Glycols; D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D014642:Vanilmandelic Acid; D002230:Carbidopa; D006719:Homovanillic Acid