title: A new alkaline phosphatase isoenzyme abnormality.
abstract: We report on a new alkaline phosphatase isoenzyme abnormality occurring as an incidental finding in a male infant aged 4 months. Isoenzyme electrophoresis on cellulose acetate showed a prominent, diffuse alkaline phosphatase staining band in the alpha 1-globulin position together with a second band in the alpha 2/beta region and minor 'trailing' in the intermediate alpha 2 region. Normal liver and bone alkaline phosphatase were absent and intestinal phosphatase was not detected. On acrylamide gel electrophoresis a marked origin band was detectable suggesting the presence of high molecular weight enzyme. In addition, a series of compact bands in the alpha 2/beta position was present cathodal to the usual liver and bone isoenzymes. Total alkaline phosphatase activity was marginally elevated and was heat labile, L-phenylalanine resistant and partially L-homoarginine and L-leucine sensitive.
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D001847:Bone Diseases / Q000201:enzymology; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000097:blood*; D007668:Kidney / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D009439:Neuraminidase / Q000378:metabolism; D017830:Octoxynol; D010206:Papain / Q000378:metabolism; D011092:Polyethylene Glycols; D014357:Trypsin / Q000378:metabolism; D014552:Urinary Tract Infections / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D007527:Isoenzymes; D011092:Polyethylene Glycols; D017830:Octoxynol; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D009439:Neuraminidase; D014357:Trypsin; D010206:Papain