[Proteases of swine small intestine enterocytes. Kinetics of substrate hydrolysis and inhibition of aminopeptidase N from brush border vesicles].
The kinetics of hydrolysis of L-leucine p-nitroanilide and some p-nitrophenylalanine dipeptides by vesicular aminopeptidase N from the porcine small intestine brush border membrane was studied. It was shown that the catalytic properties of the vesicular enzyme are very similar to those known for its solubilized counterpart. Both enzymes are inhibited by o-phenanthroline, ZnCl2 and puromycin with Ki = 10(-5)-10(-6) M. The data obtained offer new possibilities for investigating the role of aminopeptidase N in the amino acid and peptide transport across the enterocyte membrane.
D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D018826:CD13 Antigens; D002712:Chlorides; D006868:Hydrolysis; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010618:Phenanthrolines / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D013379:Substrate Specificity; D013552:Swine; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D002712:Chlorides; D010618:Phenanthrolines; D017967:Zinc Compounds; D011691:Puromycin; C016837:zinc chloride; D010447:Peptide Hydrolases; D000626:Aminopeptidases; D018826:CD13 Antigens; D015032:Zinc
Proteazy énterotsitov tonkogo kishechnika svin'i. Kinetika gidroliza substratov i ingibirovanie aminopeptidazy N vezikul shchetochnoĭ kaĭmy.