[Analysis of parameters for the equation of isotherms of chemical modification for the alkylation of nucleic acids in complexes with chloroethylmethylaminobenzylidene oligonucleotides].
The coincidence between the adsorbtion isotherm E + X in equilibrium or formed from EX (association constant KX) and isotherm of the chemical modification (the dependence of the limit extent of modification r infinity biopolymer E with excess of the reagent X) calculated earlier. Schemes (1) and (2) are given. It was shown that r infinity is identical to [EX]0/e), if (1) and (2-2) are absent due to equality of the rate constants of the conversion of X in complex and in solution, i.e. the isotherm of modification may imitate the adsorbtion isotherm. The influence of the equilibrium (2) at the initial part of the isotherm of modification is not essential, if process (1-1) is absent and y2 = k -P/k1 is small (smaller than or equal to 0.1) and y1 = (k1/k2)(kP/k-PKX) has various magnitudes within y1 x y2 smaller than or equal to 0.1. Once the parameter y1 is equal to unity, the difference between the isotherms of adsorbtion and of modification are small at the initial part in spite of equilibrium (1-1). These results indicate the KX may be calculated from the modification data given earlier in the found range of values of parameters y1 and y2, if e0 is determined by an independent way.
D000327:Adsorption; D000478:Alkylation; D055598:Chemical Phenomena; D002621:Chemistry; D009588:Nitrogen Mustard Compounds; D009841:Oligonucleotides; D012313:RNA; D013816:Thermodynamics
D009588:Nitrogen Mustard Compounds; D009841:Oligonucleotides; D012313:RNA
Analiz parametrov uravneniia izotermy khimicheskoĭ modifikatsii dlia alkilirovaniia nukleinovykh kislot v kompleksakh s khlorétilmetilaminobenzilidenoligonukleotidami.