Liquid-chromatographic assay of urinary 5-hydroxy-3-indoleacetic acid, with electrochemical detection.
After extraction with two organic solvent mixtures, urinary 5-hydroxy-3-indoleacetic acid can be assayed by "high-performance" liquid chromatography on a reversed-phase column, with electrochemical detection. Compared to the nitrosonaphthol method (J. Biol. Chem. 216: 499, 1955), this method is more specific for detection of patients with carcinoid tumors.
D002276:Carcinoid Tumor / Q000175:diagnosis* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D012016:Reference Values
D006897:Hydroxyindoleacetic Acid