Antibody-dependent redistribution and release of immunological complexes in cells of human chronic leukemias.
Redistribution and the ability for removing immunological complexes in CLL and CGL cells treated with the specific anti-leukemia antigens were investigated. The immunofluorescence method at 4 and 37 degrees C was applied at different times of cell incubation with antigens. It was shown, that incubation at 4 degrees C even carried out for 18 h, neither caused a clear aggregation nor a release of immunological complexes from the leukemia cell surface, whereas at 37 degrees C considerable differences in mobility of CLL and CGL cells were observed. In CGL cells quite a rapid redistribution followed by the removal of antigen-antibody complexes was observed, while CLL cells have shown the ability for aggregation only, without clear polarization and without releasing the formed complex from the cell surface.
D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D006801:Humans; D007106:Immune Sera; D007152:Immunologic Capping; D007945:Leukemia, Lymphoid / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007106:Immune Sera