Activation of human platelets by antibodies to thymocytes and beta 2-microglobulin. III. Effect of cell absorptions on the platelet aggregating and lymphocytotoxic activities of HATG and SA beta 2mG.
Horse anti-human thymocyte globulin (HATG) and sheep anti-human beta-2-microglobulin globulin (SA beta 2-mG), both known to have immunosuppressive potency, were quantitatively absorbed by a number of human cells, then, tested for platelet aggregating and lymphocytotoxic reactivities. Peripheral blood lymphocytes, lymphoblastoid T- and B-cell lines, platelets and spermatozoa could remove varying amounts of both reactivities from HATG and SA beta 2mG. Daudi cells were effective in reducing the lymphocyte and some platelet reactivity of HATG but did not affect those of SA beta 2mG. Erythrocytes had no absorption capacity for either antibody. The qualitative and quantitative differences in absorbing with various cells on the platelet aggregating and lymphotoxic activities of HATG and SA beta 2mG indicate the high specificity and direct action of the antibodies on platelets and lymphocytes.
D000042:Absorption; D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology; D001609:Beta-Globulins / Q000494:pharmacology*; D001792:Blood Platelets / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004912:Erythrocytes / Q000276:immunology; D006736:Horses; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D012756:Sheep; D013094:Spermatozoa / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000494:pharmacology*
D000961:Antilymphocyte Serum; D001609:Beta-Globulins; D001613:beta 2-Microglobulin