Studies on the cytotoxicity, mutagenicity and chromosomal aberration-inducing activity of aclacinomycin A in cultured mammalian cells.
The cytotoxicity mutagenicity and chromosomal aberration-inducing activity of aclacinomycin A were studied using cultured mammalian cells. Aclacinomycin A was found to be a potent inhibitor of RNA synthesis in HeLa S3 cells. The survival curve in the presence of aclacinomycin A exhibited a concentration- and time-dependent cytocidal effect, and showed a sigmoidal shoulder. A cytofluorometric study suggested that aclacinomycin A caused a temporary blockage of cell cycle progression. In mutation assay using V79 cells, aclacinomycin A was shown to induce 8-azaguanine-resistant mutation, being several times more mutagenic than adriamycin. The induction of chromosomal aberrations by aclacinomycin A was also demonstrated. When cells from various sources were treated with aclacinomycin A, they were affected at a similar concentration with the exception of human normal fibroblasts, which were a little less sensitive than the others.
D015250:Aclarubicin; D000818:Animals; D000903:Antibiotics, Antineoplastic; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D005470:Fluorometry; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D009153:Mutagens; D009279:Naphthacenes / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis
D000903:Antibiotics, Antineoplastic; D009153:Mutagens; D009279:Naphthacenes; D012313:RNA; D015250:Aclarubicin