[Evaluation of the accuracy of precipitation methods for HDL cholesterol determination].
Several methods for HDL-Cholesterol determination have been proposed; the over-floating cholesterol evaluation after non-HDL lipoproteins precipitation by polyanions with bivalent cations or neutral polymers is practical and not expensive, but it's accuracy and precision must be strictly control led. The Authors, after a review and a quality-check of the most used methods, conclude that the best accuracy for HDL-Cholesterol determination is provided by Dextransulphate-Mg-chloride and Phosphotungstate-Mg-chloride at pH 7,5.
D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL; D016264:Dextran Sulfate; D003911:Dextrans; D005069:Evaluation Studies as Topic; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008274:Magnesium; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid
D008076:Cholesterol, HDL; D003911:Dextrans; D008075:Lipoproteins, HDL; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D016264:Dextran Sulfate; D002784:Cholesterol; D008274:Magnesium
Valutazione dell'accuratezza dei metodi di precipitazione per il dosaggio del colesterolo delle HDL.