Interspecies exchange of beta 2-microglobulin and associated MHC and differentiation antigens.
Radiolabeled human beta 2-microglobulin (beta 2m) can bind to mouse histocompatibility (H-2) antigens on the cell surface or to partially purified H-2 antigens in solution. The complexes containing human beta 2m and H-2 antigens from C3H (H-2k) mice could be immunoprecipitated specifically with alloantisera, rabbit anti-H-2 xenoantisera, and with monoclonal H-2-specific antibodies. Specific association with H-2 antigens was also observed with other haplotypes. The only exception was B10.D2 (H-2d) from which complexes containing human beta 2m could only be precipitated with anti-H-2-xenosera. Thus radiolabeled human beta 2m can be used as a specific label for mouse H-2 antigens in precipitation and radioimmunoassays. The application of this finding extends to major histocompatibility complex antigens of other species, and to differentiation antigens with primary association with beta 2m.
D000818:Animals; D001609:Beta-Globulins / Q000276:immunology*; D003429:Cross Reactions; D006183:H-2 Antigens / Q000276:immunology*; D006527:Hepatolenticular Degeneration / Q000276:immunology; D006801:Humans; D007163:Immunosorbent Techniques; D007676:Kidney Failure, Chronic / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008806:Mice, Inbred AKR / Q000276:immunology; D008809:Mice, Inbred C3H / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology*
D001609:Beta-Globulins; D006183:H-2 Antigens; D001613:beta 2-Microglobulin