Rate of movement and composition of rapidly transported proteins in regenerating olfactory nerve.
In a previous study, three successive groups of regenerative fibers, growing initially at 5.8, 2.1, and 0.8 mm/day, were observed in the regenerating garfish olfactory nerve. In the present study, fast axonal transport in the most rapidly regenerating axons (phase I and II) has been examined. Rapid transport in phase I fibers occurs at a velocity of 208 +/- 9 mm/day at 23 degrees, a rate identical to that measured in intact nerves. This first phase of regenerating fibers represents only 3 to 5% of the original axonal population, but each fiber appears to contain 6 to 16 times more transported radioactivity than an axon in an intact nerve. Subcellular distribution of rapidly moving material in phase I and II fibers was closely related to the distribution obtained in intact nerves. Small but significant differences indicate a shift of the transported radioactivity from a heavier to a light axonal membranous fraction. This shift might be characteristic of the immature membrane of a growing axon. The polypeptide distribution of transported radioactivity was also very similar to that of a normal nerve, with most of the radioactivity associated with high-molecular-weight polypeptides.
D000818:Animals; D001370:Axonal Transport; D005399:Fishes; D008970:Molecular Weight; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009832:Olfactory Nerve / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D009419:Nerve Tissue Proteins