title: An ultrastructural study of vascular proliferation and vascular alkaline phosphatase activity in the developing cerebral cortex of the rat.
abstract: Alkaline phosphatase cytochemistry was employed to study the distribution of this enzyme in blood vessels during vascular differentiation and maturation during the postnatal development of the rat cerebral cortex. Enzyme reaction product was present in early vascular sprouts, and also throughout the subsequent maturation and differentiation of capillaries and arterial vessels. Cerebral capillaries appeared to be patent soon after the fusion of a sprout tip with another vessel; no evidence for delayed or synchronous opening was obtained. The distribution of alkaline phosphatase reaction product in vessel walls changed during vascular maturation. In vascular sprouts, reaction product was found mainly in the narrow lumen. As vessels became patent, reaction product appeared also on abluminal surfaces, at first chiefly in the narrow spaces between overlapping vascular cells. As vessels matured, reaction product became more generally distributed around the abluminal surface. In relatively mature capillaries and arterial vessels, it was restricted largely to endothelial cell surfaces and the spaces between smooth muscle cells. The significance of this distribution is unknown. Some possible explanations, including the possibility of artifact, are discussed. No alkaline phosphatase reaction product was found in differentiated veins.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001808:Blood Vessels / Q000648:ultrastructure*; D002196:Capillaries / Q000201:enzymology; D002536:Cerebral Arteries / Q000201:enzymology; D002540:Cerebral Cortex / Q000098:blood supply*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic; D051381:Rats
chemical_list: D000469:Alkaline Phosphatase