Analysis of embryonic induction by using cell lineage markers.
Three distinct inductive interactions have been demonstrated in early embryos of Xenopus laevis: mesoderm induction, dorsalization and neural induction. The experiments were done with grafts from embryos uniformly labelled with passive cell lineage markers, either FITC-lysine-dextran (FLDx) or horseradish peroxidase (HRP), which allow the provenance of regions to be determined down to the single cell level. In each case the fate of the target tissue in the presence of the appropriate inductor was quite different from the fate in normal development.
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D003911:Dextrans; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D005306:Fertilization; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D008648:Mesoderm / Q000502:physiology; D009420:Nervous System / Q000196:embryology; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D014981:Xenopus
D003911:Dextrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; C044226:fluorescein isothiocyanate lysine-dextran; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate