title: Molecular cloning and analysis of cDNA sequences derived from poly A+ RNA from barley endosperm: identification of B hordein related clones.
abstract: A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005983:Glutens; D001467:Hordeum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D012639:Seeds
chemical_list: D004274:DNA, Recombinant; D010940:Plant Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D005983:Glutens