Selective staining of dictyosome-like structures (DLS) from spermatocytes of the guinea pig using phosphotungstic acid at low pH.
Dictyosome-like structures (DLS) occur abundantly in primary spermatocytes of the guinea pig. DLS superficially resemble dictyosomes of Golgi apparatus in that they consist of stacked cisternae and react similarly to some cytochemical markers. DLS saccules are also present in residual bodies and in the cytoplasmic droplet of the sperm, but the stacked configuration (or dictyosome form) is seldom present at these stages of development. A mixture of 1% phosphotungstic acid in 10% chromic acid selectively stains the DLS and DLS saccules of guinea pig germ cells. The thick cisternae of spermatid Golgi apparatus and the sperm plasma membrane also stain, but endoplasmic reticulum and the parts of the Golgi apparatus other than the thick cisternae do not stain. The specificity of the stain is retained in crude homogenates as well as in purified cell fractions and may be helpful in identification of DLS in cell fractionation studies. Additionally, the information obtained provides clues to the origin and fate of DLS in the developing mammalian germ cells.
D000818:Animals; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D010772:Phosphotungstic Acid; D013090:Spermatocytes / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*; D013194:Staining and Labeling
D010772:Phosphotungstic Acid