Establishment of a long-term cultured suppressor T-cell line specific for guanosine.
Induction of suppressor cells specific for guanosine in C57BL/6 mice with syngeneic spleen cells coupled with guanosine was demonstrated. Spleen cells from mice that had been tolerized by the injection of guanosine-coupled syngeneic spleen cells (G-SC) were repeatedly stimulated with mitomycin C-treated G-SC (MMC-G-SC) in vitro. These cells effectively suppressed the secondary in vitro response to guanosine-keyhole limpet haemocyanin (G-KLH) but did not suppress the response to sheep erythrocytes (SRBC). From these cell populations a long-term-cultured suppressor T-cell line specific for guanosine was established by using interleukin-2 and MMC-G-SC. This cell line suppressed the response to G-KLH but did not suppress the response to trinitrophenyl-KLH and SRBC. The suppressive effect was completely eliminated by treatment with anti-Thy 1.2 and complement.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D005260:Female; D006151:Guanosine / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology; D018800:Thy-1 Antigens; D013997:Time Factors
D000954:Antigens, Surface; D018800:Thy-1 Antigens; D006151:Guanosine