Effects of diamfenetide on metabolic and excretory functions of Fasciola hepatica in vitro.
The effect of diamfenetide (DFT) on the time course of production of end-products of glucose metabolism, tissue ATP levels and NH3 production by adult Fasciola hepatica in vitro was determined. Products of glucose metabolism are increased between 6 and 24 hr incubation in 10(-4) M DFT. Tissue ATP levels and NH3 production are decreased during this time period. The observed metabolic effects of DFT are manifested at a much later time after drug exposure than previously described membrane-disruptive events indicating that metabolic effects of DFT on F. hepatica may be secondary to the initial effects on surface membranes.
D000083:Acetanilides / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D003957:Diamfenetide / Q000494:pharmacology*; D005210:Fasciola hepatica / Q000378:metabolism* / Q000502:physiology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D011506:Proteins / Q000378:metabolism; D013997:Time Factors
D000083:Acetanilides; D005232:Fatty Acids, Volatile; D007773:Lactates; D011506:Proteins; D002245:Carbon Dioxide; D019344:Lactic Acid; D000641:Ammonia; D000255:Adenosine Triphosphate; D003957:Diamfenetide