Long-lasting effects of electroconvulsive shock on the pattern of poly(A)-RNA synthesis in rabbit cerebral cortex.
The pattern of poly(A)-associated [poly(A)+] RNA synthesis was studied in rabbit cerebral cortex in the period following a single electroconvulsive shock (ECS). Labeled uridine was injected into the brain 2 and 4 hr after ECS and the animals sacrificed 1 hr later. Total and poly(A)+ RNA were then prepared from cortical nuclei and microsomes and analyzed. The amounts of newly synthesized total and poly(A)+ RNA in nuclei and microsomes appeared to be close to the control. However, the pattern of newly synthesized poly(A)+ nuclear RNA appeared to be still displaced toward the high molecular weights as it was in the early post-ECS period. The result indicates a long-lasting disturbance of brain poly(A)+-RNA metabolism by ECS.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004597:Electroshock; D005260:Female; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D011061:Poly A / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger; D011817:Rabbits; D012640:Seizures / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA