Identification of antigenically related polypeptides at centrioles and basal bodies.
An antigen localized at the centriolar region has been identified by indirect immunofluorescence studies in African green monkey kidney, human, hamster, rat, and mouse cells. The antigen consists of two polypeptides of 14,000 and 17,000 daltons. A related antigen is also present at the basal body region in ciliated cells from chicken, cat, mouse, pig, steer, and rabbit trachea and from rabbit fimbria. Immunoelectron microscopy shows that the immunoreactive antigen is indeed located in the region around the basal bodies of ciliated cat tracheal cells. Thus, we have found an antigen that is common to a variety of cell types from many different animal sources and is specifically associated with both centrioles and basal bodies. The possible role of the antigen in differentiation is discussed.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002502:Centrioles / Q000276:immunology / Q000648:ultrastructure*; D002522:Chlorocebus aethiops; D002923:Cilia / Q000276:immunology / Q000648:ultrastructure*; D006224:Cricetinae; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D009940:Organoids / Q000648:ultrastructure*; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D000939:Epitopes; D011506:Proteins