title: Mechanisms of the Feulgen acid hydrolysis.
abstract: Our present knowledge of the two main reactions responsible for the form of the Feulgen hydrolysis curve is summarized. A suggestion that hydrolysis slightly above room temperature and in 5 M HCl is preferable is made and discussed. A formula making it possible to calculate the appropriate hydrolysis time at any normally used acid concentration and temperature is presented.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000378:metabolism; D006651:Histocytochemistry; D006868:Hydrolysis; D013194:Staining and Labeling
chemical_list: D004247:DNA