[Incorporation C14-glycine and H3-methionine into total and cytoplasmic proteins of rat skeletal muscles in insulin deficiency and glucocorticoid hormone excess].
It was shown that insulin stimulated and hydrocortisone inhibited 14C'glycine and 3H methionine incorporation into the skeletal muscle polysome, cytosol and total proteins of rats alloxan diabetes. Hydrocortisone inhibitory effect was slightly diminished after insulin administration when both hormones were used simultaneously. The blood glucose content of diabetic rats decreased under insulin action and rose after hydrocortisone use. It was concluded that the inhibition of skeletal muscle protein metabolism may be caused not only by insulin deficiency, but also by glucocorticoid hormone excess.
D000496:Alloxan; D000818:Animals; D002250:Carbon Radioisotopes; D003600:Cytosol / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D007328:Insulin / Q000172:deficiency*; D008297:Male; D008715:Methionine / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000648:ultrastructure*; D011132:Polyribosomes / Q000378:metabolism*; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D005938:Glucocorticoids; D007328:Insulin; D009124:Muscle Proteins; D012269:Ribosomal Proteins; D014316:Tritium; D000496:Alloxan; D008715:Methionine; D005998:Glycine; D006854:Hydrocortisone
Vkliuchenie 14C-glitsina i 3H-metionina v obshchie i tsitoplazmaticheskie belki skeletnykh myshts krys pri defitsite insulina i izbytke gliukokortikoidnykh gormonov.