[Evidence of autapses in vitro by electron microscopy combined with iontophoretic intracellular injection of peroxidase].
The existence of autapses, i. e. of synapses between a neuron and its own axon, is demonstrated in vitro by means of Horseradish peroxidase (HRP) iontophoretic filling, combined with electron-microscopic (EM) analysis. After the injection of one single Purkinje cell per explant (Kitten cerebellum), the ultrastructural observation (serial thin sections) reveals the presence of both pre- and post-synaptic labelled structures. Axodendritic autapses are thus illustrated and their significance is discussed in view of earlier morphological and electrophysiological data.
D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000648:ultrastructure*; D002415:Cats; D003712:Dendrites / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D008845:Microinjections; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*; D011689:Purkinje Cells / Q000648:ultrastructure*; D013569:Synapses / Q000648:ultrastructure*
D006735:Horseradish Peroxidase
Mise en évidence d'autapses in vitro par la microscopie électronique combinée à l'injection iontophorétique intracellulaire de peroxydase.