Heterogeneity of soluble T cell products. I. Precursor frequency and correlation analysis of cytotoxic and immune interferon (IFN-gamma)-producing spleen cells in the mouse.
In this study, the precursor frequency, relationship and activity of murine splenic cytotoxic and immune interferon (IFN-gamma)-producing cells has been determined. C57BL/6 spleen cells were activated by concanavalin A (Con A) and subsequently grown in microcultures under limiting dilution conditions. The progeny of cells plated in microcultures was divided and tested for (a) total cytotoxic activity on EL4 (H-2b) tumor target cells in the presence of Con A to nonspecifically attach the killer to the target cells, and (b) the quantity of IFN-gamma released by Con A stimulation. Cytotoxic T lymphocytes and IFN-gamma-producing cells were present in Con A-activated C57BL/6 spleen cells at a high frequency of approximately 1 out of 3 and 1 out of 2 cells, respectively. IFN-gamma could be released from both noncytotoxic and a large fraction of cytotoxic T cells and the cytotoxic activity was not necessarily associated with IFN-gamma release. In a few selected cultures with a progeny of cells plated at a low cell number (up to 10/well) very high IFN-gamma titers (greater than 10 000 U/ml) could be found. These results provide the first frequency estimate of IFN-producing cells and are discussed with respect to the physiological role of IFN-gamma release from T cells.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D007372:Interferons / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008934:Mitogens; D051381:Rats; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D008934:Mitogens; D003208:Concanavalin A; D007372:Interferons