Structural relationships between carrier epitopes and antigenic epitopes on hen egg-white lysozyme.
The spatial relationship for T-B cell cooperation between antigenic epitopes and carrier epitopes on the antigen molecule was studied. Two mono-DNP substituted derivatives of hen egg-white lysozyme (HEL), DNP1-33HEL and DNP1-96HEL were used as antigens; the former is dinitrophenylated only at lysine-33 and the latter only at lysine-96. Fragment peptides of HEL were used to induce specific T cells to the respective sites of the antigen. Adoptive cell-transfer experiments of site-specific T cells and DNP-primed B cells directly showed that multiple distinct carrier epitopes for T cells could help the antibody responses to the single antigenic epitope for B cells and that a single carrier epitope could help antibody responses to multiple antigenic epitopes. T cells primed with a synthetic peptide SP34-54 (corresponding to sequence 34-54 of HEL) co-operated with DNP-primed B cells on challenge with DNP1-96HEL, but not with DNP1-33HEL.
D019297:2,4-Dinitrophenol; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D004140:Dinitrophenols / Q000276:immunology; D004529:Egg White; D000939:Epitopes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008958:Models, Molecular; D009113:Muramidase / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014475:Uncoupling Agents / Q000276:immunology
D004140:Dinitrophenols; D000939:Epitopes; D014475:Uncoupling Agents; D009113:Muramidase; D019297:2,4-Dinitrophenol