[In vitro studies on the fixation dependent artifact formation in electron microscope autoradiography].
The binding of 14C-labeled L-fucose to bovine serum albumin by glutaraldehyde was investigated. Separation of protein-bound radioactivity from free precursor was performed either by TCA precipitation, thin layer chromatography or gel chromatography on Sephadex columns. For a positive control, [14C] fucose was replaced by [14C] leucine which has repeatedly been reported to be subjected to artifactual retention in glutaraldehyde-fixed tissue. It is shown that, under conditions where substantial amounts of free leucine are bound to albumin, radioactivity from L-[14C]fucose is not detectable in the protein fractions.
D001345:Autoradiography; D002250:Carbon Radioisotopes; D005643:Fucose; D005976:Glutaral; D007930:Leucine; D008854:Microscopy, Electron / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling
D002250:Carbon Radioisotopes; D012710:Serum Albumin, Bovine; D005643:Fucose; D007930:Leucine; D005976:Glutaral
In-vitro-Studien zur fixierungsbedingten Artefaktbildung in der elektronenmikroskopischen Autoradiographie.