Bacterial synthesis of a novel human leukocyte interferon.
A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. LeIF B exhibits markedly different specificities from another bacterially synthesized human leukocyte interferon, LeIF A.
D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering; D006801:Humans; D007372:Interferons / Q000096:biosynthesis* / Q000235:genetics; D007962:Leukocytes / Q000276:immunology*; D010957:Plasmids
D004274:DNA, Recombinant; D007372:Interferons