title: An experimentally-derived model for the secondary structure of the 16S ribosomal RNA from Escherichia coli.
abstract: Ribonucleoprotein fragments are isolated by mild ribonuclease digestion of E. coli 30S ribosomal subunits, and are deproteinized and subjected to a second partial digestion. Base-pairing between the resulting small RNA fragments is investigated using the two-dimensional gel electrophoresis procedure already reported (see Ref. 1). The interactions thus found are incorporated into a secondary structure model covering approximately 80% of the 16S RNA.
mesh_terms: D001483:Base Sequence; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000032:analysis*; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000302:isolation & purification; D006163:Ribonuclease T1
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D006163:Ribonuclease T1