Staining of DNA with uranylacetate in hydrolysed ultrathin sections.
Rat liver and hepatoma cells fixed with formaldehyde, embedded into Epon and treated on sections with 5 n HCl and then with aqueous uranylacetate show preferential DNase-sensitive reaction. The reaction is highly dependent upon proper fixation, hydrolysis improves its specificity. The binding of the contrast with DNA is of ionic nature. Because of its simplicity, sufficient contrast and resolution the suggested technique is recommended for ultrastructural studies of DNA-containing substrates.
D000818:Animals; D004247:DNA / Q000032:analysis*; D006652:Histological Techniques; D008099:Liver / Q000648:ultrastructure*; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D051381:Rats; D013194:Staining and Labeling; D014501:Uranium
D009942:Organometallic Compounds; C005460:uranyl acetate; D014501:Uranium; D004247:DNA