Correspondence of homologies in amino acid sequence and tertiary structure of protein molecules.
According to the method developed previously (Kubota, Y., Takahashi, S., Nishikawa, K. and Ooi, T. (1981) J. Theor, Biol. 91, 347-361), homology among proteins may be estimated quantitatively. We extended the method to investigate the relationship of an amino acid sequence to its teritary structure and identify homologous segments which have homologous native conformations in proteins. First, we selected proper indices for the computation of correlation coefficients from 32 properties inherent to amino acids, such as hydrophobicity. The arithmetic average of correlation coefficients using six indices gave rise to a good correlation for the CD- and EF-hand regions (Ca2+ binding sites) in carp parvalbumin, but poor ones for other segments. We then applied the method to homologous proteins, the three-dimensional structures of which are known: horse hemoglobin alpha-chain and beta-chain; cytochrome c and c2; serine proteases, chymotrypsinogen and elastase; alpha-lytic protease and protease A from prokaryotic organisms. The results show that the sequence homology estimated by the present method has a good correspondence to the homology in three-dimensional structures and therefore the method is promising for the identification of important sites in sequences which have similar native conformations. For an example of the application of the method, two sequences of human interferon, one from fibroblast and the other from leukocyte, are compared, suggesting functional sites in the molecule.
D000595:Amino Acid Sequence; D000596:Amino Acids; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002918:Chymotrypsin; D003580:Cytochromes; D010450:Endopeptidases; D005163:Factor Analysis, Statistical; D006454:Hemoglobins; D006801:Humans; D007372:Interferons; D008433:Mathematics; D010320:Parvalbumins; D011487:Protein Conformation; D012697:Serine Endopeptidases
D000596:Amino Acids; D003580:Cytochromes; D006454:Hemoglobins; D010320:Parvalbumins; D007372:Interferons; D010450:Endopeptidases; D012697:Serine Endopeptidases; D002918:Chymotrypsin