title: Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport.
abstract: Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB--HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals were identified in the cytoplasm of labeled cells in the proximal portions of the dendrites, and in myelinated axons. It is demonstrated that the TMB--HRP method is well suitable to analyze the ultrastructure of labeled cells and their synaptology.
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001560:Benzidines; D004525:Efferent Pathways / Q000502:physiology*; D006168:Guinea Pigs; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D010544:Peroxidases; D014793:Visual Cortex / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D001560:Benzidines; C020515:N,N,N',N'-tetramethylbenzidine; D006735:Horseradish Peroxidase; D010544:Peroxidases