The influence of preset integer intervals at the densitometer unit of the television texture analysis system (TAS) on DNA quantification.
The relative DNA content of Feulgen stained nuclei, or concentrations of stoichiometrically binding dyes can be determined by the television texture analysis system. This determination is based on the measurement of areas at preset integer intervals of transmittance. The method includes an unavoidable error in DNA quantification caused by the smallest preset integer value of transmittance at the densitometer unit. The error at the TAS is calculable by a theoretical reduction of the preset intervals for delta T. The calculation results in a correcting factor of 0 less than or equal to 4.1% of the whole measured area of a nucleus. The revised relative DNA content can now be calculated by D=E.A.1.041.
D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D006801:Humans; D008433:Mathematics; D010783:Photometry; D013194:Staining and Labeling; D013690:Television
D004247:DNA