Boradeption: a new procedure for transferring water-insoluble agents across cell membranes.
A new process has been developed which is called "Boradeption" to signify boronic acid--dependent phase transfer of water-insoluble agents. Highly fluorescent boronic acid dervatives, FluoroBoras, are solubilized with a physiologically compatible carrier buffer containing a receptor group for boronate adduct formation. The system can be used to stain living cells. In another variation of the Boradeption concept, an insoluble reporter molecule containing a boronate receptor is solubilized with a carrier buffer containing a boronic acid functional group. The boronate-receptor complexes, which are in dynamic equilibrium, can be designed as vital stains and reagents for a variety of biological and medical applications.
D000818:Animals; D001692:Biological Transport; D001896:Boron Compounds / Q000627:therapeutic use; D001897:Boronic Acids / Q000627:therapeutic use; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002863:Chromogenic Compounds / Q000378:metabolism; D006224:Cricetinae; D005347:Fibroblasts; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D051381:Rats; D013194:Staining and Labeling
D001896:Boron Compounds; D001897:Boronic Acids; D002863:Chromogenic Compounds; D005456:Fluorescent Dyes