title: Combined immunological and histochemical analysis of skin and lymph node lesions in histiocytosis X.
abstract: The immunological phenotype of the cells involved in skin and lymph node lesions from two cases of histiocytosis X (H-X) were analysed by immunofluorescence techniques using combinations of heterologous and monoclonal antisera to Ia-like antigen and human cortical thymocyte (HTA-1) determinant. These cells were also characterised by a new technique using simultaneous immunofluorescence and enzyme histochemistry for acid phosphatase (ACPase). The major cell type in the lesions was found to express the same Ia+, HTA-1+ phenotype as normal epidermal Langerhans' cells (LC) and was unreactive for ACPase. Additional cell types included Ia-, HTA-1- multinucleate giant cells and residual lymphoid populations. These findings endorse previous concepts that H-X is a proliferation of abnormal LC and emphasise the heterogeneous nature of the cells involved in the disease.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006681:HLA-D Antigens; D006646:Histiocytosis, Langerhans-Cell / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology*; D012867:Skin / Q000276:immunology / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006681:HLA-D Antigens; D000949:Histocompatibility Antigens Class II; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases