Lysosomal enzyme activities and RNA turnover rates in growing and nongrowing WI-38 and HeLa cells.
Activities of three lysosomal enzymes--acid RNase. N-acetyl-beta-D-glucosaminidase and acid phosphatase--were determined during the growth cycles of WI-38 and HeLa cells, as well as in radiation-arrested WI-38 cells. In confluent and growth-arrested cultures of WI-38 cells, the lysosomal RNase increased six- to sevenfold; glucosaminidase, four- to fivefold; and phosphatase, two- to threefold. In HeLa cells, the lysosomal enzymes also increased in confluent cultures, but less than twofold; and the RNase level increased only transiently. In both WI-38 and HeLa cells, the rate of RNA breakdown also increased as cultures approached confluency. The rate of turnover of RNA, like the level of acid RNase, was higher in WI-38 cells than in HeLa cells (4 d half-life compared to 8 d). The increase in acid RNase could be prevented by incubation of cells in NH4Cl, but the rate of turnover in the presence of NH4Cl increased just as much when cells became confluent or stopped growth. The content of acid RNase could be changed more than 10-fold without altering the rate of RNA turnover. It is suggested that the increase in enzyme level is more important for possible autophagy or increased digestion of engulfed RNA, rather than for normal RNA turnover, when growth stops.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000135:Acid Phosphatase / Q000378:metabolism*; D000643:Ammonium Chloride / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D006367:HeLa Cells; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*
D000643:Ammonium Chloride; D012313:RNA; D012260:Ribonucleases; D000135:Acid Phosphatase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase