A physical study of the ovine genome.
The ovine genome has been divided into some seventy-five fractions using 3,6-bis(acetatomercurimethyl)dioxane (BAMD) in conjunction with Cs2SO4 density-gradient-equilibrium centrifugation. Distinct macromolecular populations detected have buoyant densities in CsCl of 1.700, 1.707, 1.714, 1.716, 1.717, 1.721, 1.724 and 1.725 g/cm3. The 1.724 g/cm3 material appears in a number of non-contiguous fractions obtained from BAMD-Cs2SO4 centrifugation suggesting its presence at a number of different sites in the genome. Within two regions of buoyant density (1.701 g/cm3 to 1.707 g/cm3 and 1.708 g/cm3 to 1.717 g/cm3) the analyses were unable to resolve discrete populations.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification / Q000494:pharmacology; D004146:Dioxanes; D008767:Methylmercury Compounds; D012313:RNA / Q000302:isolation & purification*; D012756:Sheep / Q000235:genetics*
D004146:Dioxanes; D008767:Methylmercury Compounds; C006759:3,6-bis(acetatomercurimethyl)dioxane; D012313:RNA; D004247:DNA