Improved fluorescence method for identifying sex chromatin in formalin-fixed tissue.
An improved fluorescence method for demonstrating sex chromatin in formalin-fixed tissue is described. Deparaffinized sections are pretreated with pronase, then acid hydrolysis, before staining with quinacrine dihydrochloride and mounting in buffered Gelvatol. Optimized fluorescence of nuclei allows recognition of either Y chromatin or Barr body in interphase nuclei. The method is a useful tool in sexing early conceptuses and teratomata using an extensive paraffin-block storage resource.
D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D007399:Interphase; D008856:Microscopy, Fluorescence / Q000379:methods*; D012728:Sex Chromatin; D013194:Staining and Labeling
D005557:Formaldehyde