Biosynthesis of intestinal microvillar proteins. Expression of aminopeptidase N along the crypt-villus axis.
The expression of pig small-intestinal aminopeptidase N (EC 3.4.11.2) along the crypt-villus axis was studied in tangential sections of [35S]-methionine-labelled, organ-cultured explants. The only detectable molecular forms of aminopeptidase N along the crypt-villus axis were polypeptides of Mr 140 000 and 166 000, representing the enzyme in a transient and mature form respectively. The synthesis was at a very low level in the crypt region in experiments with labelling periods ranging from 10 min to 3 h. These findings indicate that crypt cells are not fully committed to the expression of aminopeptidase N, either in its mature or in any other immunoreactive molecular form. The expression of aminopeptidase N was markedly stimulated by dexamethasone (1 microgram/ml). During labelling periods of 3 h, dexamethasone caused an approximately threefold increase in the expression of the enzyme in the crypt cells and a moderate increase of about 20% in the villus cells. Whereas the latter can possibly be ascribed to a general protective effect of dexamethasone on villus architecture, these experiments indicate that crypt cells of mucosa from adult individuals exhibit the same sensitivity to glucocorticoids as does the intestinal epithelium during the prenatal and early postnatal phase.
D000626:Aminopeptidases / Q000096:biosynthesis*; D000818:Animals; D018826:CD13 Antigens; D003907:Dexamethasone / Q000494:pharmacology; D007120:Immunochemistry; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000648:ultrastructure; D008715:Methionine / Q000378:metabolism; D008871:Microvilli / Q000201:enzymology; D009924:Organ Culture Techniques; D013552:Swine
D003907:Dexamethasone; D008715:Methionine; D000626:Aminopeptidases; D018826:CD13 Antigens