Response of EMT6 multicellular tumour spheroids to hyperthermia and cytotoxic drugs.
The response of multicellular tumour spheroids of the EMT6 cell line to combinations of hyperthermia and Bleomycin (BLM) or Adriamycin (ADM) has been investigated. Using this model system, we have demonstrated enhanced BLM cytotoxicity at 43 degrees C and also heat-induced drug tolerance to BLM at 43 degrees C. ADM cytotoxicity was not significantly increased after 43 degrees C x 1 h but after 6 h at 42 degrees C greatly enhanced cell-killing was evident. These results are discussed in relation to our previous data for EMT6 cells growing either as monolayer cultures in vitro or as solid tumours in mice.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D006358:Hot Temperature; D051379:Mice; D008954:Models, Biological; D009374:Neoplasms, Experimental / Q000473:pathology*; D013997:Time Factors
D001761:Bleomycin; D004317:Doxorubicin