title: Determination of procainamide and N-acetylprocainamide in biological fluids by high-pressure liquid chromatography.
abstract: A modification of a high-pressure liquid chromatographic method for the simultaneous determination of procainamide and N-acetylprocainamide in plasma is described. The deficieicies in the specificity of the existing method were overcome by replacing the cation-exchange column and the mobile phase. The recovery and reproducibility of both procainamide and N-acetylprocainamide from human, dog, and rat plasma and urine spiked with either compound were excellent in the concentration range of 0.05--10 microgram/ml for plasma and 0.5--20 microgram/ml for urine. The comparison of this method with a specific extraction method for sets of plasma samples from human subjects and rats receiving N-acetylprocainamide and procainamide, respectively, showed no statistically significant difference.
mesh_terms: D000120:Acecainide / Q000032:analysis* / Q000097:blood / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D006801:Humans; D011342:Procainamide / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D013045:Species Specificity
chemical_list: D000120:Acecainide; D011342:Procainamide