Purification of immunologically functional subsets of human Ia-like antigens on a monoclonal antibody (Q5/13) immunoadsorbent.
Serologic and immunochemical asays have shown that the monoclonal antibody Q5/13 recognizes an antigenic determinant expressed on a subset of human Ia-like antigens. Testing with a panel of HLA typed B lymphoid cells has shown that this determinant is different from those defining the serologic polymorphism of HLA-DR antigens. The monoclonal antibody Q5/13 has been used to purify subsets of human Ia-like antigens, which are immunologically functional. These reagents should facilitate the characterization of structural and functional properties of human Ia-like antigens.
D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000494:pharmacology*; D001402:B-Lymphocytes; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007164:Immunosorbents / Q000494:pharmacology*; D008970:Molecular Weight
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007164:Immunosorbents