Inhibition of rat renin by antibody elicited against pure mouse renin and the effect on blood pressure.
Antibodies raised against mouse submaxillary renin showed affinity for rat renal renin. The affinity was characterized by enzyme kinetics in vitro. The antibodies and the purified Fab fragments showed total inhibition of rat renin, with a Ki of 6.5 +/- 0.7 x 10(-8)mol/l for the Fab fragments. The effect of enzymatic inhibition on blood pressure in anaesthetized rats was compared with converting enzyme inhibition. The antibodies showed a significant effect on blood pressure, with a depressor effect corresponding to that of the converting enzyme inhibitor [SQ 20 881]. The reduction of mean arterial pressure was from 116 +/- 3 to 108 +/- 3 mmHg for the antibodies and to 107 +/- 4 mmHg for the converting enzyme inhibitor. The pressor response of infused rat renal renin was totally blocked by the antibodies while pressor responses of angiotensin II were unchanged.
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001794:Blood Pressure / Q000187:drug effects*; D005260:Female; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007700:Kinetics; D051379:Mice / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000276:immunology*; D013722:Teprotide / Q000494:pharmacology
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D000804:Angiotensin II; D013722:Teprotide; D012083:Renin