title: Reversibility of IgE-mediated rat mast cell desensitization.
abstract: Desensitization in normal rat peritoneal mast cells was induced by subthreshold and suboptimal levels of antirat IgE under normal calcium buffer conditions. Low doses of anti-IgE which triggered 0-10% net histamine release, initiated up to 100% desensitization. The rate of desensitization was highly variable in different rats, resulting in an irregular decay of releasability over the first 30 min. After 120-150 min of desensitization, rat mast cells in 3 of 5 experiments spontaneously regained releasability. We hypothesize that IgE-mediated desensitization in rat mast cells may represent a potentially reversible physiologic blockade in the degranulation process.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D002469:Cell Separation; D003888:Desensitization, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology*; D010718:Phosphatidylserines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D010718:Phosphatidylserines; D007073:Immunoglobulin E