Specific T helper and suppressor cells and factors induced in vitro to the hapten NP.
Nitrophenyl (NP)-specific helper cells and suppressor cells were induced in vitro using NP-T4 bacteriophage as antigen. These cells could mediate their effects also by secreted effector molecules, helper and suppressor factors. The function of both NP-specific helper and suppressor cells was abolished by treatment with anti-Thy1.2 plus C', but they were not retained on nylon wool columns, suggesting that NP-specific helpers and suppressors were T cells. The membrane phenotype of both NP-specific helper and suppressor cells was found to be Ly1+2+I-J+(I-A-). The secreted effector molecules, helper and suppressor factors which mediate helper or suppressor function, bound to NP immunoadsorbents and are NP-specific in their function. They do not have conventional Ig determinants, but both bear determinants coded by the I-J subregion of H-2. The unusual phenotype of NP-specific helper and suppressor cells is discussed, as is the potential use of these hapten-specific T cells and their secreted effector molecules in increasing our understanding of T-cell receptors, effector molecules and the fine specificity of the interacting network in the regulation of immune responses.
D000818:Animals; D002478:Cells, Cultured; D000939:Epitopes; D006241:Haptens / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009596:Nitrophenols / Q000276:immunology*; D010648:Phenylacetates; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013604:T-Phages
D000939:Epitopes; D006241:Haptens; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid