Interaction of somatomedin-C with an antibody directed against the synthetic C-peptide region of insulin-like growth factor-I.
Insulin-like growth factor-I is a human plasma peptide with strong structural homology to human prosinsulin. This peptide has been classified as a somatomedin on the basis of its biological actions and growth hormone dependence. We have generated an antibody to the synthetic 12 amino acid C-peptide region of insulin-like growth factor-I and used it to compare three somatomedin preparations to insulin-like growth factor-I. We also compared these somatomedin preparations to insulin-like growth factor-I using the standard SM-C RIA. We conclude that insulin-like growth factor-I and somatomedin-C behave identically in these two RIA's and are very similar if not identical molecules.
D000595:Amino Acid Sequence; D002096:C-Peptide / Q000097:blood; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D007106:Immune Sera; D010455:Peptides / Q000097:blood; D011384:Proinsulin; D011863:Radioimmunoassay; D013002:Somatomedins / Q000097:blood
D002096:C-Peptide; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides; D011384:Proinsulin; D013002:Somatomedins