title: Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.
abstract: Spleen cells from mice immunized with purified carcinoembryonic antigen (CEA), an important tumor marker of human carcinomas, were fused with the mouse myeloma cell line P3-NSI/1-Ag4. Out of the 400 hybrids obtained, 2 secreted antibodies reacting specifically with two different antigenic determinants present on CEA molecules. They were cloned and established as permanent hybridoma cell lines. These antibodies, which have relatively high-affinities and can be produced in unlimited amounts, will be useful both for the immunochemical characterization of CEA and as a standard reagent for the identification of this antigen in human tissues and body fluids.
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000302:isolation & purification*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002272:Carcinoembryonic Antigen; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006822:Hybrid Cells / Q000276:immunology; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007700:Kinetics; D051379:Mice
chemical_list: D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G