Antigen and receptor-driven regulatory mechanisms. VI. Demonstration of cross-reactive idiotypic determinants on azobenzenearsonate-specific antigen-binding suppressor T cells producing soluble suppressor factor(s).
The first detectable suppressor T cell (Ts) arising after i.v. administration of azobenzenearsonate- (ABA) conjugated syngeneic spleen cells to A/J mice has been studied for its receptor specificity and ability to produce soluble suppressor factor(s). This cell, termed Ts1, has a specific receptor for the eliciting antigen ABA, as demonstrated by selective binding to ABA protein- but not TNP protein-coated plastic dishes. The activity of ABA-Ts1 can be abrogated by treatment with anti-idiotypic antibodies made against anti-ABA antibodies of A/J mice (anti-CRI), indicating that these ABA-binding cells possess a surface receptor structure sharing idiotypic determinants with antibodies of the same specificity. Finally, soluble extracts from, antigen-adherent ABA-Ts1, but not nonadherent cells from the same spleen cell population, possess suppressive activity when assayed directly for afferent suppression or tested for their ability to trigger a second population of Ts (Ts2) in naive recipients. These findings demonstrate a close concordance between a T cell surface receptor, soluble T suppressor factors, and B cell derived antibody, all capable of direct recognition of the eliciting ABA antigen.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001391:Azo Compounds / Q000276:immunology*; D001665:Binding Sites; D003429:Cross Reactions; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D012995:Solubility; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000941:Antigens; D001391:Azo Compounds; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010132:p-Azobenzenearsonate