Characterization of a human B lymphocyte-specific antigen.
A human B lymphocyte-specific antigen (B1) was identified and characterized by the use of a monoclonal antibody. By indirect immunofluorescence, cytotoxicity, and quantitative absorption, B1 was present on approximately 9% of the peripheral blood mononuclear cell fraction and >95% of B cells from blood and lymphoid organs in all individuals tested. Monocytes, resting and activated T cells, null cells, and tumors of T cell and myeloid origin were B1 negative. B1 was distinct from standard B cell phenotypic markers, including Ig and Ia antigen. Removal of the B1 positive population in peripheral blood eliminated all B cells capable or responding to pokeweed mitogen by maturation to Ig-producing cells.
D000042:Absorption; D000818:Animals; D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011043:Pokeweed Mitogens / Q000494:pharmacology
D000906:Antibodies; D000939:Epitopes; D011043:Pokeweed Mitogens