Sialyltransferase, sialic acid and sialoglycoconjugates in metastatic tumor and human liver tissue.
1. The specific activities of sialytransferase in metastatic tumor sites were 5-37% of those of uninvolved non-cancerous tissue. The non-cancerous host tissues had a slightly lower average sialyltransferase activity than that of non-pathological control livers (986 vs 1194 dpm/min/mg protein). 2. The levels of total and bound sialic acid are increased 1.4 to 7.2-fold in homogenates, supernatants and resuspended pellets of metastatic tumor sites compared to non-cancerous and non-pathological control livers. For all these tissues, 24-29% of the bound sialic acid is found on soluble components (in the 16,300 g supernatant). 3. Soluble sialoglycoconjugates from most metastatic tumor sites give gel filtration profiles different from those of non-cancerous and non-pathological control livers.
D000328:Adult; D000368:Aged; D001212:Asialoglycoproteins; D005260:Female; D060748:Fetuins; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary; D008297:Male; D008875:Middle Aged; D012794:Sialic Acids / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism*; D012995:Solubility; D014166:Transferases / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism
D001212:Asialoglycoproteins; D060748:Fetuins; D012794:Sialic Acids; D012795:Sialoglycoproteins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D014166:Transferases; D012799:Sialyltransferases