title: Histochemistry of lower vertebrate calcified structures. I. Enamel of the dogfish Squalus acanthius compared with mammalian enamel and homologous dentine.
abstract: The enameloid and dentine of Squalus acanthius have been compared histochemically with those of Bos taurus. Squalus enameloid is much less reactive to a variety of stains or reagents than dentine or bovine immature enamel but it does have positive reactions with picromethyl blue, Mallory's and Van Gieson's stains, and Alcian blue. It stains faintly with Biebrich scarlet, indicating some anionic groups. Specific reactions for tyrosine, tryptophane, lysine, histidine, arginine, and cysteine are negative. Bos immature enamel is positive for cationic, anionic, and aromatic reactive groups by all test procedures, and dentine was positive for the anionic components. Bovine maturing enamel, however, is more similar in terms of lack of reactivity to Squalus enameloid but differed because the bovine enamel was moderately positive for tyrosine; tryptophane, and anionic groups and negative with Mallory's picromethyl blue and Van Gieson's stains. A fibrous transitional area between Squalus dentine and enameloid has staining reactions characteristic of both collagen and keratins.
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D004396:Coloring Agents / Q000378:metabolism; D003743:Dental Enamel / Q000378:metabolism / Q000502:physiology*; D003804:Dentin / Q000378:metabolism / Q000502:physiology*; D004284:Dogfish / Q000502:physiology*; D005333:Fetus / Q000502:physiology; D006651:Histocytochemistry; D012754:Sharks / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents