Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
A method has been developed for the electrophoretic transfer of DNA, RNA, protein and ribonucleoprotein particles from a variety of gels onto diazobenzyloxymethyl (DBM) - paper. Conditions for the electrophoretic transfer of these macromolecules have been optimized to allow for nearly quantitative transfer and covalent coupling. DNA and RNA electrophoretically transferred to DBM-paper retain their ability to hybridize with specific probes. The high efficiency of transfer and the high capacity of DBM-paper for nucleic acids makes possible the sensitive detection of specific nucleotide sequences. Similar efficiency is achieved in electrophoretic transfer and covalent coupling of proteins to DBM-paper. Macromolecules can also be electrophoretically transferred and bound to DBM-paper incapable of covalent bond formation. Their elution from the paper in high salt provides a new and useful preparative method for isolation of DNA, RNA and protein.
D003090:Coliphages / Q000032:analysis; D004279:DNA, Viral / Q000302:isolation & purification*; D003979:Diazonium Compounds; D004586:Electrophoresis; D004926:Escherichia coli / Q000032:analysis; D007202:Indicators and Reagents; D008970:Molecular Weight; D009492:Neurospora crassa / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009698:Nucleoproteins / Q000302:isolation & purification*; D011132:Polyribosomes / Q000032:analysis; D012313:RNA / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000302:isolation & purification; D012331:RNA, Fungal / Q000302:isolation & purification
D004279:DNA, Viral; D003979:Diazonium Compounds; D007202:Indicators and Reagents; D009698:Nucleoproteins; D012329:RNA, Bacterial; D012331:RNA, Fungal; C015794:diazobenzyloxymethyl groups; D012313:RNA