An axial periodic fibrillar arrangement of antigenic determinants for fibronectin and procollagen on ascorbate treated human fibroblasts.
Fibronectin and collagens are major constituents of the cell matrix of fibroblasts. Fibronectin is a 220,000 dalton glycoprotein that mediates a variety of adhesive functions of cells examined in vitro. Fibronectin is secreted in a soluble form and interacts with collagen to form extracellular filaments. Fibronectin and procollagen type I were localized using the peroxidase anti-peroxidase method. Under standard culture conditions, fibronectin and procollagen were localized to non-periodic 10 nm extracellular fibrils, the cell membrane and plasma membrane vesicles. Ascorbate treatment of cells leads to a new larger fibril with a diameter of approximately 40 nm. Antibodies to fibronectin and procollagen I react to these native collagen fibrils with an axial periodicity of approximately 70 nm. Fibronectin is clearly associated with native collagen fibrils produced by ascorbate treated cells and there is an asymetric distribution or segregation of fibronectin on these collagen fibrils with a 70 nm axial repeat.
D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D000939:Epitopes; D005347:Fibroblasts / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D046911:Macromolecular Substances; D003012:Microbial Collagenase; D008854:Microscopy, Electron; D008970:Molecular Weight; D011347:Procollagen / Q000032:analysis*; D012867:Skin / Q000032:analysis / Q000187:drug effects
D000939:Epitopes; D005353:Fibronectins; D046911:Macromolecular Substances; D011347:Procollagen; D003012:Microbial Collagenase; D001205:Ascorbic Acid