A silver impregnation technique for the study of steroid receptors in central nervous system tissue prepared for autoradiography.
The commonly used silver stains were found to be unsatisfactory for nervous tissue processed for autoradiography. A silver impregnation procedure for central nervous system tissues prepared for the autoradiographic study of steroid receptors is described. The procedure is a combination of several silver and reticular strains made up in solutions containing dimethylsulfoxide. The technique clearly distinguishes perikarya of neurons, brain nuclei and fiber tracts without substantial loss of silver grains, and thus greatly facilitates the identification of steroid receptor nuclei at all levels of the central nervous system.
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D004121:Dimethyl Sulfoxide; D008297:Male; D009474:Neurons / Q000166:cytology; D010215:Papio; D011944:Receptors, Androgen / Q000032:analysis*; D011987:Receptors, Steroid / Q000032:analysis*; D012835:Silver Nitrate; D013116:Spinal Cord / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
D011944:Receptors, Androgen; D011987:Receptors, Steroid; D012835:Silver Nitrate; D004121:Dimethyl Sulfoxide