Antibodies to synthetic antibody-combining sites. Antibodies against a surface-simulation peptide with antibody-combining activity toward lysozyme antigenic site 3 react with lysozyme antibodies.
Recently, we reported the synthesis and immunochemistry of two peptides designed, by complementarity and surface-simulation synthesis, to mimic antibody-combining sites against two antigenic sites of lysozyme. In the present work antibodies were raised against one of these peptides, which is complementary to antigenic site 3 of lysozyme, to determine whether these antibodies will react with anti-lysozyme antibodies. Radioiodinated antipeptide antibodies were bound by immunoadsorbents of the immune IgG from two goats anti-lysozyme antisera but not by adsorbents of myoglobin, non-immune goat IgG or immune IgG of antisera against cytochrome c. The binding of anti-peptide antibodies to adsorbents of anti-lysozyme antibodies was fully inhibited by free lysozyme but not by bovine serum albumin, human hemoglobin A, horse cytochrome c or bovine ribonuclease A. Thus, antisera against an antibody-combining site can be raised by immunizing with a peptide which probably does not exist in the antibody but is designed by surface-simulation synthesis to mimic an antibody-combining site.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006041:Goats / Q000276:immunology; D007118:Immunoassay; D007074:Immunoglobulin G; D009113:Muramidase / Q000276:immunology*; D010455:Peptides / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007074:Immunoglobulin G; D010455:Peptides; D009113:Muramidase