title: Characterization of Bacillus subtilis mutants temperature-sensitive in the synthesis of ribonucleic acid.
abstract: Two mutants of Bacillus subtilis temperature-sensitive in RNA synthesis were isolated. One mutation (rna-20) was demonstrated to be an allele of a previously identified gene (Riva et al., 1976). The other mutation (rna-16) identified a different gene and was mapped near aroI. The rna-16 mutation at the permissive temperature affected the spore outgrowth process. Purified RNA polymerase from rna-16 did not show any temperature sensitivity or structural defect.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006358:Hot Temperature; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis*
chemical_list: D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases