title: Chicken fetal antigen: association with lymphoid development and differentiation.
abstract: Rabbit antisera capable of detecting chicken fetal antigen (CFA) was prepared against 1-day chick red blood cells (RBCs) and made specific by exhaustive adsorption with adult chicken peripheral RBCs (PRBCs) from the same strain. Microcytotoxicity was used to study the incidence of CFA on lymphocytes obtained from several organs at different developmental stages in the chicken. Lymphocyte-associated CFA (LA-CFA) determinants and erythrocyte-specific CFA (ES-CFA) determinants were distinguished through the use of adsorption analysis. The high incidence of CFA-positive lymphocytes found in the fetal bursa and thymus was not equaled in the peripheral organs of the spleen, cecal tonsils, and gland of Harder. CFA expression on adult lymphocytes was restricted to the thymus and peripheral blood. It is suggested that these cells may represent a subpopulation of T lymphocytes. Adult spleen, cecal tonsils, and gland of Harder were virtually devoid of CFA-bearing lymphocytes. At fetal developmental stages when greater than 94% of the bursal B cells were CFA-positive, few, if any, of the highly differentiated Harderian B cells possessed CFA. It is suggested that LA-CFA expression is dependent upon B cell differentiation and/or the bursa (central) vs gland of Harder (peripheral) microenvironment. The pattern of CFA expression on bursacytes is discussed in light of the properties of age resistance and bursal-dependent target cells associated with virally induced lymphoid leukosis.
mesh_terms: D000375:Aging; D000818:Animals; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D000939:Epitopes / Q000032:analysis; D005260:Female; D006243:Harderian Gland / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007106:Immune Sera; D008214:Lymphocytes / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D013154:Spleen / Q000276:immunology
chemical_list: D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; C041136:Ia-like antigens, avian; D007106:Immune Sera