Biochemical transformation of mouse cells by a purified fragment of marmoset herpesvirus DNA.
Although the size of marmoset herpesvirus (MarHV) DNA, estimated by velocity sedimentation in sucrose gradients, was similar to that of herpes simplex virus type 1 (HSV-1) DNA, the restriction endonuclease sites of MarHV and HSV-1 DNAs were quite different. A specific BamHI restriction fragment (6.2 x 10(6) daltons) of MarHV DNA biochemically transformed LM(TK-) mouse fibroblasts to the thymidine kinase(TK)-positive phenotype. Rabbit antisera, prepared against MarHV TK, inhibited MarHV-induced TK, but not HSV-1, HSV-2, or cellular TKs. Disc PAGE analyses and enzyme neutralization experiments with the anti-MarHV TK sera demonstrated that the TK expressed in MarHV transformants was MarHV-specific.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D000939:Epitopes; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D051379:Mice; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
D004279:DNA, Viral; D000939:Epitopes; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes