Simple manual method for harvesting granulocytes.
The method described requires no special equipment and permits the harvesting of granulocytes from collections of fresh concentrated erythrocytes with buffy coat. In contrast to the use of a cell separator, it requires little time of technologists and none of medical staff to recover an equivalent number of granulocytes. This method makes granulocyte transfusion practicable for the support of neutropenic patients even in centers with no leukopheresis program.
D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology; D007937:Leukapheresis; D007958:Leukocyte Count
D006895:Hydroxyethyl Starch Derivatives