[Changes in functional and metabolic characteristics of isolated rat heart during initial phase and long lasting perfusion].
Mechanical performance, tissue content of high-energy phosphates (ATP and CP), glycogen and phosphorylase activity were measured in isolated rat hearts either just after excision (hearts arrested by plunging in ice-cold perfusate) or during long time perfusions (6 h) at 37 degrees C. Arrested hearts exhibited : (1) higher total phosphorylase activity (a + b); (2) higher percentage of phosphorylase a; (3) lower CP content. During perfusion, high energy phosphates appeared well maintained whereas glycogen content and phosphorylase activity decreased with a significant correlation between these two parameters. The validity of this isolated heart model and possible implications of the variations in phosphorylase activity are discussed.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006003:Glycogen / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D010762:Phosphorylase a / Q000378:metabolism; D010763:Phosphorylase b / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D006003:Glycogen; D010762:Phosphorylase a; D010763:Phosphorylase b
Evolution des caractéristiques fonctionnelles et métaboliques du coeur isolé de rat durant la phase initiale de la perfusion et au cours de perfusions de longues durées.