title: Studies on the interaction of Sendai virus with liposomal membranes. Sendai virus-induced agglutination of liposomes containing glycophorin.
abstract: Liposomes constituted with the major sialoglycoprotein of human erythrocytes, glycophorin, were used as models for studies on cell-virus interactions. Liposomes composed of egg yolk phosphatidylcholine, cholesterol and glycophorin were found to interact with the paramyxovirus HVJ to form aggregates. The aggregation process was temperature dependent: it was maximal at 0 degrees C and decreased with increase of the incubation temperature. The activity of viral neuraminidase is also temperature dependent, and it increases with increase of the incubation temperature; release of N-acetylneuraminic acid was negligible at 0 degrees C. Shift-up of the incubation temperature immediately cancelled HVJ-induced agglutination of liposomes. Viruses attached to liposomes seemed to be released into the supernatant when the 'virus-liposome' complex formed at 0 degrees C was incubated at 37 degrees C, possibly as a result of breakdown of the 'binding' site by neuraminidase. The characteristics of the interaction of HVJ with liposomes containing glycophorin appeared to be phenomenologically similar to those of HVJ-cell interaction.
mesh_terms: D000371:Agglutination; D002118:Calcium / Q000494:pharmacology; D003080:Cold Temperature; D004492:Edetic Acid / Q000494:pharmacology; D006021:Glycophorins / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D010713:Phosphatidylcholines; D012794:Sialic Acids / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000494:pharmacology
chemical_list: D006021:Glycophorins; D008081:Liposomes; D010713:Phosphatidylcholines; D012794:Sialic Acids; D012795:Sialoglycoproteins; D000509:alpha-Fetoproteins; D004492:Edetic Acid; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D002118:Calcium