Preparation of covalently linked immune complexes of specific composition.
Covalently linked soluble immune complexes were prepared with a 2-stage bifunctional reagent. Complexes with antigen-antibody ratio 2 : 1 were purified and characterized. The covalent linkage and antigenic characteristics of the complexes were demonstrated.
D000818:Animals; D000936:Antigen-Antibody Complex; D002417:Cattle; D002847:Chromatography, Agarose; D003432:Cross-Linking Reagents; D003485:Cyanates / Q000494:pharmacology*; D006041:Goats; D007105:Immune Complex Diseases / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*; D005719:gamma-Globulins / Q000276:immunology
D000936:Antigen-Antibody Complex; D003432:Cross-Linking Reagents; D003485:Cyanates; D005719:gamma-Globulins; D014051:Toluene 2,4-Diisocyanate; D012710:Serum Albumin, Bovine