Retrograde axonal transport of endogenous proteins in sciatic nerve demonstrated by covalent labeling in vivo.
Extracellularly applied N-succinimidyl [2,3-3H]propionate was used in vivo to covalently label intra-axonal proteins in the rat sciatic nerve. This technique permitted a unique view of axonal transport of proteins independent of biosynthesis. The proteins detected in slow anterograde transport (1 to 2 millimeters per day) correspond to cytoskeletal proteins described in previous papers. The slowly retrogradely transported component (3 to 6 millimeters per day) was composed primarily of a single protein with a molecular weight of 68,000.
D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D005260:Female; D006652:Histological Techniques; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011422:Propionates; D051381:Rats; D012584:Sciatic Nerve / Q000378:metabolism*; D013388:Succinimides
D009419:Nerve Tissue Proteins; D011422:Propionates; D013388:Succinimides; C021749:N-succinimidyl propionate