Electrokinetic response of granulocytes to concanavalin A and succinyl-concanavalin A.
Electrophoretic light scattering has been used to study the effects of concanavalin A (Con A) and succinyl-Con A on the electrophoretic mobility distribution of resident guinea-pig peritoneal eosinophils and human peripheral blood polymorphonuclear leukocytes. In both cell types, incubation with Con A (a tetrameric lectin) decreases slightly the mean mobility and increases substantially the width of the electrophoretic mobility distribution. These effects can be abolished by alpha-methyl-D-mannoside, a hapten sugar of Con A. Succinyl Con A, a dimeric derivative, was found to have no effect on the mobility distribution. These results are strikingly similar to our previous report of the response of the resident guinea-pig macrophage (19), suggesting possible parallels in the endocytic mechanisms of these cell types.
D000818:Animals; D003208:Concanavalin A / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004586:Electrophoresis; D006098:Granulocytes / Q000187:drug effects*; D006168:Guinea Pigs; D006801:Humans; D007834:Lasers; D008766:Methylmannosides / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*; D012542:Scattering, Radiation
D008766:Methylmannosides; D003208:Concanavalin A; C019558:succinylconcanavalin A