Thiazolidine-2-carboxylate derivatives formed from glyoxylate and L-cysteine or L-cysteinylglycine as possible physiological substrates for D-aspartate oxidase.
Adducts of glyoxylate with L-cysteine or L-cysteinylglycine were found to be excellent substrates at low concentrations for beef kidney D-aspartate oxidase. Evidence is presented that cis-thiazolidine-2,4-dicarboxylate and its glycine amide are the actual substrates, and that both are converted in the enzymic reaction to 4-substituted thiazoline-2-carboxylates. The results imply that these thiazolidine derivatives are the likely physiological reactants for mammalian D-aspartate oxidase.
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002417:Cattle; D003545:Cysteine / Q000378:metabolism; D050804:D-Aspartate Oxidase; D004151:Dipeptides / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006038:Glyoxylates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011392:Proline / Q000031:analogs & derivatives* / Q000378:metabolism; D013379:Substrate Specificity; D053778:Thiazolidines
D004151:Dipeptides; D005971:Glutamates; D006038:Glyoxylates; D053778:Thiazolidines; C021760:thiazolidine-2-carboxylic acid; D001224:Aspartic Acid; C028505:cysteinylglycine; D018698:Glutamic Acid; D011392:Proline; D000594:Amino Acid Oxidoreductases; D050804:D-Aspartate Oxidase; C031150:glyoxylic acid; D003545:Cysteine