[Isolation and physical study of the 13S fragment of 16S RNA and its complex with ribosomal protein S4].
A fragment of E. coli 16S RNA has been obtained by its hydrolysis with pancreatic RNAase A coupled to Sepharose 4B. This fragment has a molecular weight of 170 000 and a sedimentation coefficient of 13S. It does not aggregate in solution and binds with the ribosomal protein S4. The 13S fragment and it complex with the protein S4 have been studied by different physical methods in the first place, by neutron scattering. It has been shown that this fragment is compact in solution. The radii of gyration of the fragment (50 +/- 3 A) and of the protein S4 within the complex (17 +/- 3 A) coincide, within limits of experimental error, with the radii of gyration for the free RNA fragment (47 +/- 2 A) and the free ribosomal protein S4 in solution (18 +/- 2 A). Hence, the conclusion is made that the compactness of the 13S fragment of the 16S RNA and the ribosomal protein S4 does not change at the complex formation. The compact 13S fragment of the 16S RNA is shown to be contrast matched in the H2O/D2O mixture containing 70% D2O which corresponds to its partial specific volume v equal to 0.537 cm3/g.
D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000032:analysis*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009502:Neutrons; D011485:Protein Binding; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012269:Ribosomal Proteins; D012542:Scattering, Radiation
D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins
Vydelenie i fizicheskoe issledovanie 13S fragmenta 16S RNK i ego kompleksa s ribosomnym belkom S4.