title: Properties of homogeneous heat-labile enterotoxin from Escherichia coli.
abstract: Recently, the heat-labile enterotoxin (LT) of Escherichia coli has been purified to homogeneity and partially characterized (Clements and Finkelstein, Infect. Immun. 24:760-769, 1979). This study extends our observations on the physicochemical properties of LT. The toxin has an isoelectric point of pH 8.0, as compared with choleragen and choleragenoid, which have isoelectric points of pH 6.75 and 7.75, respectively. Sedimentation equilibrium measurements established an approximate molecular weight for LT of 91,440. LT had an even more marked affinity than choleragen for agarose-containing matrixes in gel filtration. Of several mono- and disaccharides tested, only galactose and lactose were highly efficient in removing 125I-labeled LT from agarose-containing columns. LT dissociated into subunits (designated A and B) during gel filtration in the presence of 5 M guanidine. These subunits were immunologically distinct and possessed unique and shared antigenic determinants to the corresponding A and B subunits of choleragen. During gel filtration of LT at pH 6.5 and room temperature, a spontaneously occurring toxoid of LT, analogous to choleragenoid, was discovered and designated "coligenoid." This product contains only the B subunits of the toxin. A partial amino acid sequence of the B subunit of LT revealed a remarkable homology to the primary structure of cholera toxin B. Within the first 20 amino acids of the two chains, only 5 differ, and these differences may be attributable to single base substitutions.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D002772:Cholera Toxin; D002850:Chromatography, Gel; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D000939:Epitopes; D004926:Escherichia coli / Q000032:analysis*; D006358:Hot Temperature; D007526:Isoelectric Point; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; D002772:Cholera Toxin