A technique combining intracellular dye-marking, immunocytochemical identification and ultrastructural analysis of physiologically identified single neurons.
A new method which produces an insoluble osmophilic polymer within Lucifer Yellow-injected neurons has allowed us to develop a technique for the ultrastructural examination of electrophysiologically characterized, immunocytochemically identified single neurons. In this initial report, we examine the light- and electron-microscopic features of neurophysin-containing, pituitary-projecting neurons in the goldfish nucleus.
D000818:Animals; D006054:Goldfish; D007031:Hypothalamus / Q000166:cytology*; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D009481:Neurophysins / Q000032:analysis*; D011301:Preoptic Area / Q000166:cytology* / Q000648:ultrastructure; D013194:Staining and Labeling
D009481:Neurophysins