[Citrate method of extracting antigens from Shigella sonnei. I. Comparison with other methods].
The comparative characteristic of extracts obtained from Sh. sonnei by the method of Raynaud and Digeon and neurotoxin obtained by sedimentation with trichloroacetic acid from chloroform autolysates by the method of Mesrobeanu et al. is presented. Both preparations were shown to be similar in their antigenic structure and immunogenicity for mice and guinea-pigs. Nevertheless the extracts obtained by the citrate method contained considerably less protein and endotoxin and had no enterotoxic activity. This allows to recommend the citrate method for extracting protective antigens from Sh. sonnei.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000032:analysis; D002951:Citrates; D004731:Endotoxins / Q000032:analysis; D000939:Epitopes; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008722:Methods; D051379:Mice; D009498:Neurotoxins / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D012764:Shigella sonnei / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002951:Citrates; D004731:Endotoxins; D000939:Epitopes; D009498:Neurotoxins
Tsitratnyĭ metod ékstraktsii antigenov iz Shigella sonnei sravnenie s drugimi metodami.