Circulating autoantibodies to hidden mitochondrial antigenic determinants in mammals.
Autoantibodies to equine cytochrome c and to porcine malic dehydrogenase are shown in equine and porcine serum, respectively, by immunodiffusion. A confluence of arcs between human plasma and rabbit, horse and pig serum against these antigens suggests the presence of antibodies in human plasma to cytochrome c and malic dehydrogenase. The use of tris buffer containing a low concentration of Dodecyl sodium sulfate for cytochrome c and of glycerol for malic dehydrogenase maintains their solubility and enhances their participation in antibody-antigen immunoprecipitation possibly by unravelling the peptide chains and exposing reactive antigenic determinants hidden within the interstices of the cytochrome c and malic dehydrogenase molecules.
D000818:Animals; D001323:Autoantibodies; D001775:Blood Circulation; D011232:Chemical Precipitation; D003574:Cytochrome c Group / Q000276:immunology; D000939:Epitopes; D006736:Horses; D006801:Humans; D005779:Immunodiffusion; D008291:Malate Dehydrogenase / Q000276:immunology; D008928:Mitochondria / Q000276:immunology*; D011817:Rabbits; D013552:Swine
D001323:Autoantibodies; D003574:Cytochrome c Group; D000939:Epitopes; D008291:Malate Dehydrogenase