title: myo-Inositol action on gerbil intestine. Association of phosphatidylinositol metabolism with lipid clearance.
abstract: The synthesis and turnover of phosphatidylinositol as well as clearance were studied in the intestines of lipodystrophic gerbils treated with or without an intraperitoneal dose of myo-inositol by monitoring the incorporation of 32Pi and the retention of absorbed [1-14C] palmitic acid. 1. myo-Inositol deficiency produced an intestinal lipodystrophy with a large lipid accumulation and a decreased level of phosphatidylinositol. Upon myo-inositol repletion, the intestinal phosphatidylinositol rapidly returned to the control level by h, at which time the removal of excess lipid still remained in a lag phase. 2. myo-Inositol injection caused an increase in the incorporation of 32Pi into phosphatidylinositol mainly due to an increased phosphatidylinositol synthesis de novo. As a result, the turnover of phosphatidylinositol molecules might increase because of an expanded pool size. 3. The stimulation of phosphatidylinositol synthesis was then followed by an enhanced clearance of absorbed [14C] palmitate and by an intestinal recovery which was monitored by the loss of accumulated triacylglycerol. 4. This study indicates that myo-inositol availability appears to regulate the in vivo biosynthesis of phosphatidylinositol which, in turn, may play a crucial role in normal lipid transport across gerbil intestine.
mesh_terms: D000818:Animals; D005260:Female; D005849:Gerbillinae; D007294:Inositol / Q000172:deficiency / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D010716:Phosphatidylinositols / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis; D008061:Whipple Disease / Q000378:metabolism*
chemical_list: D010716:Phosphatidylinositols; D010743:Phospholipids; D014280:Triglycerides; D007294:Inositol