Detection of influenza virus by means of immune sera to M and RNP inner proteins in solid phase radioimmunoassay.
Solid phase radioimmunoassay (SPRIA) was shown suitable for detection of influenza virus in various biological materials using immune sera (IS) to membrane protein (MP) and ribonucleoprotein (RNP) isolated from the strain A/USSR/90/77. Either serum was able to detect influenza A viruses with different haemagglutinins (H1, H3, H7) and did not react with influenza B viruses. SPRIA detected 0.3 ng viral protein per 1 ml, i.e. its sensitivity was 20 times higher than that of the haemagglutination (HA) test. Neither serum revealed the virus in the extract from choroallantoic membrane (CAM) of infected chicken embryos. As follows from competitive radioimmunoassay (RIA), the antigenic specificity of A/PR/8/34 virus RNP differed from that of A/USSR/90/77 virus RNP.
D000914:Antibodies, Viral / Q000276:immunology*; D007158:Immunologic Techniques; D009980:Influenza A virus / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D012261:Ribonucleoproteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D008565:Membrane Proteins; D012261:Ribonucleoproteins; D014764:Viral Proteins