Serological characterization of previously unknown H-2 molecules identified in the products of the Kd and Dk region.
The molecular relationship between H-2 private and some public specificities was studied in C3H.OH (H-202) mice using surface-antigen re-distribution methods. Besides the Kd- and Dk-region antigens, which can be capped by antisera against the private and public specificities characteristic for a given allele, a previously unknown type of molecule was found in the products of both the Kd and Dk regions. These can be capped by the respective anti-private serum but not by antisera against some public specificities. The two Kd-region molecules are provisionally named H-2K1d and H-2K2d. We detected them on H-202 (Kd, Id, Sd, Dk) and also on H-2dx (Kd, If, Sf, Ddx) T lymphocytes. Similarly, the two types of molecules detected on the products of the Dk region are provisionally named H-dD1k and H-2D2k. The serological characteristics of these molecules are described. When compared with the products of the Dd region, in which we previously described three different molecules (H-2Dd, H-2Md, and H-2Ld), the mutual relationship between H-2K1d and H-2K2d as well as between H-2D1k and H-2D2k appears to be similar to that between H-2Dd and H-2Md. In the absence of relevant recombinants or informative biochemical data, it is, however, difficult to establish homology between molecules produced by different K- and D-region alleles.
D000042:Absorption; D000818:Animals; D002874:Chromosome Mapping; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D006238:Haploidy; D007106:Immune Sera / Q000494:pharmacology; D007152:Immunologic Capping; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D014176:Protein Biosynthesis
D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera