Detection of circulating immune complexes by a C1q-microplate ELISA system.
A microscale ELISA system, designated as the 'C1q-micro ELISA,' has been developed for the measurement of circulating immune complexes. The sensitivity of the method ranged between 0.1 and 3 microgram equivalents of aggregated human gamma-globulins. The procedure requires small quantities of serum and reagents, and it can be performed with rapidity without the loss of accuracy and reproducibility.
D000936:Antigen-Antibody Complex; D003166:Complement Activating Enzymes / Q000378:metabolism*; D015922:Complement C1q; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D005719:gamma-Globulins / Q000378:metabolism
D000936:Antigen-Antibody Complex; D005719:gamma-Globulins; D015922:Complement C1q; D003166:Complement Activating Enzymes