Histamine release induced by neurotensin from rat peritoneal mast cells.
Neurotensin induced the release of histamine from both purified and non-purified rat peritoneal mast cells by a non-cytotoxic mechanism. It was effective at a concentration as low as 10(-8) M. The dose-response curve for the neurotensin effect was triphasic: and initial gentle rise, a plateau (2.5 X 10(-7) -2.5 X 10(-6) M) and a second rise. Disodium cromoglycate markedly, but benzalkonium chloride only slightly, inhibited the neurotensin-induced release. The effect of neurotensin was markedly different from that of substance P, bradykinin and compound 48/80 with respect to both the dose-response curve and the sensitivity to benzalkonium chloride. These inducers of histamine release showed somewhat different effects with changes in pH and temperature. Their actions did not require extracellular Ca2+. The results indicate that neurotensin is a potent histamine releaser acting directly on mast cells. Its mode of action may be different from those of the other substances tested.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001202:Ascitic Fluid / Q000166:cytology; D001548:Benzalkonium Compounds / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D009496:Neurotensin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D001548:Benzalkonium Compounds; D009496:Neurotensin; D003189:p-Methoxy-N-methylphenethylamine; D000968:Antimycin A; D004205:Cromolyn Sodium; D002118:Calcium