Purified low-molecular-weight protein kinase from murine sarcoma virus particles catalyzes tyrosine phosphorylation endogenously but phosphorylates cellular proteins at serine.
The low-molecular-weight (LMW) protein kinase associated with high-titer murine sarcoma virions have been extensively purified by ammonium sulfate fractionation. Bio-Gel P-100 gel filtration, DEAE-cellulose and carboxymethyl cellulose chromatography. The purified enzyme migrates as a 16K polypeptide in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The enzyme catalyzes phosphotransfer with ATP as a phosphate donor to various exogenously added proteins as acceptors; it requires Mg2+ and is independent of cyclic AMP. The enzyme preparation catalyzes a low level of phosphorylation in the absence of any exogenously added substrate and forms phosphotyrosine. However, in the presence of acceptor protein molecules including total soluble cytoplasmic proteins of murine sarcoma virus-transformed mouse cells, the phosphorylated end products contain predominantly phosphoserine. The virion-associated enzyme also shows a preference for phosphorylating certain polypeptides in the soluble cytoplasmic extracts of murine sarcoma virus-transformed cells.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism*; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D009053:Sarcoma Viruses, Murine / Q000201:enzymology*; D012694:Serine / Q000031:analogs & derivatives*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D011506:Proteins; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D012694:Serine; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D008274:Magnesium