The use of the peroxidase reaction to obliterate staining of eosinophils by fluorescein-labelled conjugates.
The staining of eosinophils by fluorescein conjugates can be obliterated by utilising the peroxidase activity of the eosinophil. The brown reaction product of 3,4,3',4'-tetraaminobiphenyl hydrochloride (DAB) with hydrogen peroxide was more effective than Lendrum's stain. A satisfactory alternative to the possibly carcinogenic DAB was 2,7-fluorenediamine.
D004804:Eosinophils; D005452:Fluoresceins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A; D013194:Staining and Labeling; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology
D005452:Fluoresceins; D007070:Immunoglobulin A; D004124:p-Dimethylaminoazobenzene