title: Chromatid lesions and chromatid core morphology.
abstract: A silver-staining technique revealed the core morphology of metaphase chromosomes of irradiated CHO cells with chromatid lesions (breaks, gaps). These cells were photographed before and after silver staining. As a rule, the core was not continuous in chromatid gaps, suggesting that the chromatid is broken in many so-called gaps. Ten cytogeneticists who were asked to classify chromatid gaps and breaks from photographs of chromosome lesions before silver core staining agreed in only 19 of 53 cases.
mesh_terms: D000818:Animals; D002460:Cell Line; D002842:Chromatids / Q000528:radiation effects* / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005720:Gamma Rays; D007621:Karyotyping; D010053:Ovary; D012834:Silver; D013194:Staining and Labeling
chemical_list: D012834:Silver