Formation of alpha-1,2- and alpha-1,3-linked mannose disaccharides from dolichyl mannosyl phosphate by rat liver membrane enzymes.
Dolichyl mannosyl phosphate and GDPmannose were active substrates for the transfer of mannose to methyl-alpha-D-mannose, p-nitrophenyl-alpha-D-mannose, and free mannose with rat liver microsomal membranes. The products formed during dolichyl mannosyl phosphate incubation with methyl-alpha-D-mannose or with mannose were alpha-linked. The disaccharides formed by incubation of dolichyl mannosyl phosphate or GDPmannose with mannose were identified by paper chromatography and electrophoresis as mannose-alpha-1,2-mannose and mannose-alpha-1,3-mannose. synthesis of each product was dependent on the assay conditions used and was most markedly affected by the presence of detergent. Transfer of mannose from either substrate to form mannose-alpha-1,3-mannose was severely inhibited by Triton X-100.
D000818:Animals; D004187:Disaccharides / Q000096:biosynthesis; D004287:Dolichol Monophosphate Mannose / Q000378:metabolism*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008361:Mannosidases; D008362:Mannosides / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*; D051381:Rats
D004187:Disaccharides; D008362:Mannosides; D011105:Polyisoprenyl Phosphate Sugars; D006155:Guanosine Diphosphate Mannose; D004287:Dolichol Monophosphate Mannose; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D008361:Mannosidases