Effect of interferon preparations on the uptake of non-opsonized Escherichia coli by mouse peritoneal macrophages.
Uptake of non-opsonized Escherichia coli by mouse peritoneal macrophages (MPM) is influenced by pre-treatment of MPM with homologous interferon preparations. Incubation of MPM with 10(2)-10(3) units of interferon per ml for 18 hours resulted in a 30-70 per cent increase in the phagocytosis rate as well as maximal phagocytic capacity. This effect was time dependent. At least seven hours of interferon pre-treatment and a phagocytosis period of 90 minutes were necessary for a statistically significant increase of the phagocytosis. Treatment of MPM with high concentrations of interferon (i.e. more than 10(4) units per ml) had a depressive effect on the phagocytosis. Both the enhancing and the depressive effects were characterized by the standard physico-chemical properties of interferons. Both effects were species specific. The phagocytosis enhancing effect was neutralized by anit-interferon serum.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D004926:Escherichia coli; D007372:Interferons / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D010587:Phagocytosis; D013997:Time Factors
D007372:Interferons