Radioimmunoassay of human pancreatic amylase in serum and urine.
The purification of human pancreatic amylase (EC 3.2.1.1) and the development of a radioimmunoassay for this enzyme is described. The enzyme was purified from a normal pancreas obtained at autopsy and antibodies were produced in rabbits. The purified pancreatic amylase was radioiodinated and the radioimmunassay was developed. Analytic recoveries in serum and urine specimens were 99 and 96%, respectively, and day-to-day precision was less than 10%. The assay gave a 20% cross-reactivity with salivary amylase.
D000681:Amylases / Q000032:analysis* / Q000097:blood / Q000652:urine; D000818:Animals; D003429:Cross Reactions; D006801:Humans; D010179:Pancreas / Q000201:enzymology*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012463:Saliva / Q000201:enzymology
D000681:Amylases