Purification of reverse transcriptase from avian retroviruses using affinity chromatography on heparin-sepharose.
Reverse transcriptase from Rous sarcoma virus and avian myeloblastosis virus was purified by a rapid two-step procedure using chromatography on phosphocellulose and heparin-Sepharose. The resulting enzyme was homogeneous, had a high specific activity and was free of contaminating nucleases. This procedure has been adapted to small-scale preparation of enzyme from mutant virus containing thermolabile reverse transcriptase, and is equally suitable for large-scale enzyme purification.
D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D001358:Avian Sarcoma Viruses / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D006493:Heparin; D008722:Methods; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification*; D012685:Sepharose; D014764:Viral Proteins
D014764:Viral Proteins; D006493:Heparin; D012685:Sepharose; D012194:RNA-Directed DNA Polymerase