Suppressive effect of human interferon on memory cells primed to different HLA-D specificities.
In primed lymphocyte typing (PLT), lymphocytes will respond with accelerated kinetics and increased magnitude when confronted with the same stimulating cells in a secondary MLC, or with other cells sharing their HLA-D determinants. Human lymphoblastoid interferon (HIF) caused a dose-dependent inhibition of 3H-TdR uptake in PLT cultures. Flow cytometry cell cycle analysis of HIF-treated PLT cells has shown reduced number of cells in S phase with a concomitant rise in the number of cells in the G0-G1 phase; this suggests that interferon has blocked the progression of PLT cells into S phase. Also HIF inhibited cells proliferation in response to a third party sharing a common determinant, Dw5 with the primary stimulator cell. HIF may be a potential immuno-suppressive agent for transplantation. The well-known antiviral effect of HIF might also prove useful in prevention opportunistic viral infection during transplantation.
D001402:B-Lymphocytes / Q000276:immunology; D002453:Cell Cycle; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D007166:Immunosuppressive Agents; D007372:Interferons / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D007372:Interferons