A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans.
A modified collagenase digestion method is described for the isolation of large numbers of islets from the canine pancreas (approximately 3,500 islets/g). The islets isolated by this technique remained viable and released insulin in response to secretagogues after one week of maintenance in tissue culture. Islets isolated from a single donor pancreas were re-implanted into the spleen of the same animal made diabetic by subtotal pancreatectomy and two injections fo streptozotocin. Hyperglycemia was corrected in two dogs and decreased in a third dog followed for 30 days. The islet isolation method is described, therefore, provides a sufficiently large yield of viable islets from one donor pancreas to correct or improve diabetes in a recipient animal.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D004285:Dogs; D005215:Fasting; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine