SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000.
The large T protein coded by the early region of simian virus 40 (SV40) is involved in the induction and maintenance of cell transformation. It is not clear which properties of T are important in causing the transformation, since the protein is multifunctional. To clarify the action of T proteins in transformation, we have prepared several monoclonal antibodies directed against different regions of the T molecule. One of these antibodies, DL 3C4, recognizes an antigenic determinant on T that is also present on a host cell protein of molecular weight 68,000. This cross-reactive 68K protein is located within the nucleus of all mammalian cell types examined and has a characteristic granular distribution as shown by immunofluorescence. The antigenic determinant recognized by DL 3C4 is resistant to denaturation. Studies on Adeno-SV40 hybrid viruses show that the antigenic site on T is coded for by sequences located between 0.44 and 0.29 on the SV40 map.
D000956:Antigens, Viral / Q000235:genetics; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003429:Cross Reactions; D000939:Epitopes; D005814:Genes, Viral; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology*; D013539:Simian virus 40 / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D011506:Proteins