Influence of two different fixatives on the identification of plasma cells in human rectal mucosa.
Plasma cells in sections of bisected human rectal biopsy specimens, fixed in two alternative fixatives, were enumerated after staining by an indirect immunoperoxidase procedure intended to demonstrate immunoglobulin-containing cells. The counts of immunoperoxidase-positive plasma cells were significantly higher after fixation in formol sublimate than after fixation in formol saline. Formol sublimate appears to be a more reliable fixative than formol saline for specimens of rectal mucosa in which quantitation of plasma cells, stained for intracellular immunoglobulin by an immunoperoxidase technique, is intended.
D001706:Biopsy; D003109:Colonic Diseases, Functional / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis; D007413:Intestinal Mucosa / Q000473:pathology*; D012007:Rectum / Q000473:pathology*; D013194:Staining and Labeling
D007136:Immunoglobulins