Effects of concanavalin A and cholera toxin on epidermal cAMP and migration rate during wound closure in adult newts.
Following removal of a skin patch from each hind limb of a series of adult newts, the limbs were explanted into small dishes of Holtfreter solution containing various combinations of test drugs. Later, the amount of wound epithelium that formed on each limb was determined using a planimeter on wound tracings obtained with the aid of a drawing tube-equipped microscope. Exposure of migrating cells to the plant lectin, concanavalin A (con A), lowered cyclic AMP (cAMP) levels and depressed migration. Exposure to cholera toxin and theophylline (CTX) significantly elevated cAMP levels and significantly depressed migration rate. Exposure of CTX-treated cells to con A tended to lower CTX-elevated cAMP levels while depressing the migration rate well beyond the depression caused by CTX alone. These results provide further evidence that cAMP can regulate the rate of newt epidermal cell migration. They also show that the inhibitory effect of con A on motility in these cells is independent of its effects on cAMP.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002772:Cholera Toxin / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D012455:Salamandridae; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
D003208:Concanavalin A; D002772:Cholera Toxin; D013806:Theophylline; D000242:Cyclic AMP