Comparison of chemiluminescence and absorptiometry in enzyme immunoassays for protein quantification.
The use of chemiluminescence in competitive binding assays for human serum albumin, human alpha-fetoprotein and human immunoglobulin G and in double antibody sandwich enzyme immunoassays for cytomegalovirus and herpes simplex virus increased the sensitivity of the detection of antigen or antibody 16- to 95-fold above that obtained by conventional absorptiometric methods.
D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D008163:Luminescent Measurements; D011506:Proteins / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis; D000509:alpha-Fetoproteins / Q000032:analysis
D007074:Immunoglobulin G; D011506:Proteins; D012709:Serum Albumin; D014764:Viral Proteins; D000509:alpha-Fetoproteins