Clones of human cytotoxic T lymphocytes derived from an allosensitized individual: HLA specificity and cell surface markers.
By planned immunization of a volunteer, two stable (greater than or equal to 6 months), specific, alloreactive cytolytic T-cell clones have been established from his peripheral blood lymphocytes. One clone reacts with all serologically defined HLA-Cw3 cells from our panel, whereas the other defines a split within the serological HLA-B40 specificity. The two cytotoxic clones are SmIg-negative, E-rosette positive, EA and EAC rosette-negative, HLA-A, -B and -C- positive, and also HLA-DR- or 'Ia like'-positive. In addition, they present very similar patterns of iodinated cell surface molecules as analysed by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), contrasting with that of an EBV cell line derived from the same donor.
D000328:Adult; D000818:Animals; D000954:Antigens, Surface; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005260:Female; D006680:HLA Antigens; D006801:Humans; D007114:Immunization; D008213:Lymphocyte Activation; D008297:Male; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D000939:Epitopes; D006680:HLA Antigens; D010455:Peptides