Macrophage depletion using Cytodex.
Cytodex (microcarrier) beads were adapted for use as an adhering surface to deplete macrophages from a mouse spleen cell population. Non-adherent cells recovered from the incubation of one mouse spleen with 50 mg of Cytodex showed good viability and had markedly reduced proliferative response to the T cell mitogens phytohemagglutinin and concanavalin A. Response to lipopolysaccharide was, however, less clearly affected. Peritoneal exudate cells, and adherent cells recovered from the Cytodex by trypsinization, were able to restore the mitogenic response of non-adherent cells.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010835:Phytohemagglutinins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D014357:Trypsin / Q000494:pharmacology
D003911:Dextrans; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D003208:Concanavalin A; C025614:sephadex; D014357:Trypsin