Expression of HLA-A, -B, and -C and beta 2-microglobulin antigens in human choriocarcinoma cell lines.
Immunoprecipitation of [35S]methionine-labeled cell extracts and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that cells from two human choriocarcinomas JaR and BeWo contained beta 2-microglobulin. The JaR cells, and one subline of BeWo, did not express surface HLA-A, -B, and -C (HLA-ABC) antigens, nor did JaR contain the antigens when cell-free extracts were measured in a sensitive radioimmunoassay. It may be concluded that the lack of expression of HLA-ABC antigens in choriocarcinoma (and in the trophoblast) is not a result of lack of beta 2-microglobulin, as is the case for the Burkitt's lymphoma cell line, Daudi.
D000818:Animals; D001609:Beta-Globulins / Q000032:analysis*; D002460:Cell Line; D002822:Choriocarcinoma / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000032:analysis*
D001609:Beta-Globulins; D006680:HLA Antigens; D001613:beta 2-Microglobulin