IgG antibodies: in vitro blocking activity of IgE mediated reactions.
Sera from 177 atopic patients treated by immunotherapy were screened in order to detect IgD antibodies against a purified fraction of Lollium perenne (fractions C). IgD antibodies were found in 23% of the subjects. Eight of these sera were selected and their IgD isolated by means of immunoabsorption. The in vitro biological activity of the IgD antibodies against fraction C was tested by three methods: basophil degranulation, histamine release and RAST inhibition test. In all these assays, the data obtained indicated a possible blocking effect of the IgD antibodies upon the IgE in vitro mediated reactions.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D001491:Basophils / Q000378:metabolism; D001667:Binding, Competitive; D006636:Histamine Release; D006801:Humans; D007072:Immunoglobulin D / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D007073:Immunoglobulin E / Q000378:metabolism*; D007138:Immunoglobulin delta-Chains / Q000032:analysis; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000628:therapy
D000888:Antibodies, Anti-Idiotypic; D007072:Immunoglobulin D; D007138:Immunoglobulin delta-Chains; D007073:Immunoglobulin E