Generation of lipopolysaccharide-induced interferon in spleen cell cultures. I. Genetic analysis and cellular requirements.
Incubation of murine spleen-cell cultures with lipopolysaccharide (LPS) induces interferon (IF) production. Maximal IF levels are obtained after incubation with 100 microgram/ml for 10 h. Two inbred mouse strains differing in their ability to generate LPS-induced IF in spleen-cell cultures were used: C3H/eB, which generates high levels of IF (about 60 units/ml), and C3H/HeJ, which fails to generate detectable quantities of IF. In a genetic analysis these strains were hybridized and IF production was determined in spleen-cell cultures from F1 and F2 generations, and from backcrosses of F1 hybrids to parent strains. The results indicate that, in parent strains, a single dominant autosomal gene is responsible for differences in IF production in spleen cultures. LPS-induced IF in spleen-cell cultures resists pH 2 for as long as 48 h, but is labile to heating at 56 degrees C for 30 min. Both macrophages and lymphocytes must be present in cultures for generation of LPS-induced IF. By using mixed cultures of macrophages and lymphocytes from C3H/eB and C3H/HeJ mice, it was shown that macrophages have to interact directly with LPS to enable IF production in the cultures.
D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis* / Q000235:genetics; D009052:Leukemia Virus, Murine; D007942:Leukemia, Experimental; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology
D007369:Interferon Inducers; D008070:Lipopolysaccharides; D007372:Interferons