title: Soluble blue as a counterstain in the Ziehl-Neelsen procedure--A brief communication.
abstract: A technique is described using soluble blue instead of the conventional methylene blue as a counterstain for the Ziehl-Neelsen procedure. This increases the intensity of blue staining of non-bacterial contaminants and is useful in monitoring the progress of purification of M. leprae from host tissue.
mesh_terms: D001431:Bacteriological Techniques; D008751:Methylene Blue; D009166:Mycobacterium leprae / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D008751:Methylene Blue