title: Use of ruthenium red for visualization of the regenerating cell wall in yeast protoplasts.
abstract: Ruthenium red was used to stain the surface of fresh and regenerating protoplasts in Saccharomyces cerevisiae. The presence of a dense layer, 10--40 nm wide, was demonstrated on the surface of most of the fresh protoplasts. This layer could be removed neither by repeated washings of protoplasts nor ;by their centrifugation. Ruthenium red proved to be a very useful dye for regenerating walls since it stained both the amorphous and the fibrillar components, but to a different degree, thus permitting their easy differentiation.
mesh_terms: D002473:Cell Wall / Q000502:physiology / Q000648:ultrastructure; D011523:Protoplasts / Q000648:ultrastructure; D012430:Ruthenium Red; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D013194:Staining and Labeling
chemical_list: D012430:Ruthenium Red