A haemagglutination and haemagglutination inhibition test for bluetongue virus.
Haemagglutination of bluetongue virus (BTV) was demonstrated for the first time by making use of a purified preparation of the virus. The reaction was found to be independent of variations in the pH, temperature, buffer system and origin of the erythrocytes used in the test. A haemagglutination inhibition test, subsequently developed, was demonstrated to be serotype specific. The storage of the virus for indefinite periods was facilitated by lyophilization of BTV in the presence of a low concentration of sucrose.
D000956:Antigens, Viral / Q000032:analysis; D001820:Bluetongue virus / Q000276:immunology*; D002021:Buffers; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006386:Hemagglutination Tests / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012087:Reoviridae / Q000276:immunology*; D013696:Temperature
D000956:Antigens, Viral; D002021:Buffers; D000939:Epitopes