A monoclonal antibody for large-scale purification of human leukocyte interferon.
A clone of hybrid myelomas (NK2), secreting a mouse monoclonal antibody to human leukocyte interferon, has been isolated. The antibody neutralizes the antiviral activity of the interferon and, when covalently attached to a solid support and used as an immunoadsorbent, allows interferon purification of up to 5,000-fold in a single step.
D000906:Antibodies; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D002999:Clone Cells / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007163:Immunosorbent Techniques; D007372:Interferons / Q000276:immunology / Q000302:isolation & purification*; D007962:Leukocytes / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis; D009194:Myeloma Proteins / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D009194:Myeloma Proteins; D007372:Interferons