Antigen-pulsed macrophage monolayers as specific immunoabsorbents: selective absorption of murine T cells committed to soluble protein antigen.
Antigen-pulsed macrophage (MO) monolayers in petri dishes were successfully employed as immunoabsorbents, for both the depletion and enrichment of T cells which proliferated upon exposure to soluble ovalbumin (OA). This procedure was shown not to deplete T cells reactive to a second antigen, and similarly MO monolayers pulsed with other antigens failed to deplete the T-cell preparation of OA-reactive cells. Employing brief incubation with trypsin/ethylenediamine tetracetic acid (EDTA), up to 2 X 10(6) OA-binding T cells could be recovered from each petri dish.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D000939:Epitopes; D005260:Female; D007164:Immunosorbents / Q000276:immunology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000941:Antigens; D000939:Epitopes; D007164:Immunosorbents; D010047:Ovalbumin