Peanut agglutinin affinity chromatography of Trypanosoma cruzi glycoproteins.
Glycoproteins from Trypanosoma cruzi epimastigotes have been extracted by diiodosalycilic acid and lithium salts, and phenol-water biphasic partition. Peanut agglutinin has been used in a one step preparative method for fractionating the total extract in order to separate the so-called galactose-terminal glycoproteins. The different fractions have been studied by SDS electrophoresis, ultracentrifugation and immunoelectrophoresis techniques. The experimental immunogenicity, antigenicity and specificity of the PNA affinity fractions has been evaluated.
D000818:Animals; D000917:Antibody Formation; D002846:Chromatography, Affinity; D003429:Cross Reactions; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D006041:Goats; D007114:Immunization; D037102:Lectins; D007891:Leishmania / Q000276:immunology; D008970:Molecular Weight; D019887:Peanut Agglutinin; D011817:Rabbits; D014345:Trypanosoma / Q000276:immunology; D014349:Trypanosoma cruzi / Q000032:analysis*
D000939:Epitopes; D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin