Heterogeneity of human chromosome 9 constitutive heterochromatin as revealed by sequential distamycin A/DAPI staining and C-banding.
Distamycin A/DAPI staining and sequential C-banding of human lymphocyte chromosomes reveals the regular occurrence of differentially staining subfractions of chromosome 9 constitutive heterochromatin. These subfractions are regionally organized as two subsegments: a distal one, which fluoresces brightly with DAPI after preincubation with distamycin A and a proximal one, which stains intensely with Giemsa after sequential C-banding. Observations are presented that indicate an occasionally independent genetic behavior of these heterochromatin subfractions.
D000578:Amidines; D002871:Chromosome Banding; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure*; D004214:Distamycins; D005456:Fluorescent Dyes; D006570:Heterochromatin / Q000648:ultrastructure*; D006801:Humans; D007211:Indoles; D008214:Lymphocytes / Q000166:cytology; D008677:Metaphase; D013194:Staining and Labeling
D000578:Amidines; D004214:Distamycins; D005456:Fluorescent Dyes; D006570:Heterochromatin; D007211:Indoles; C007293:DAPI