Dissection of the B10.D2 anti-H-2Kb cytolytic T lymphocyte receptor repertoire.
The B10.D2 cytolytic T lymphocyte (CTL) receptor repertoire specific for the H-2Kb alloantigen has been studied by determining the reactivity patterns of monoclonal CTL against a panel of seven different H-2Kb mutants. The repertoire is extremely diverse and contains a minimum of approximately 50 different specificities against unique antigenic determinants on the H-2Kb molecule. Each specificity appears at a maximum frequency of 1 in 18,000 CTL precursors. These studies have also served to dissect the antigenic composition of the H-2Kb molecule. Very few CTL clonotypes share recognition of all the mutants, thereby indicating the lack of conservation of a b-type antigenic region. In addition, the degree to which each mutant shares antigenic determinants with the standard H-2Kb molecule has been determined.
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D011971:Receptors, Immunologic / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D011971:Receptors, Immunologic