Cytoplasmic viral DNA synthesis in exogenous infection of murine leukemia virus: effect of interferon and cycloheximide.
Cytoplasmic viral DNA synthesis can be followed efficiently by [3H]thymidine labeling of cells exogenously infected with Moloney murine leukemia virus. Both the negative and the positive strands of viral DNA reached their maximal level in the cytoplasm at 3.5 h postinfection. Interferon treatment before infection markedly reduced the amount of viral DNA formed during the first 3.5 h, but led to a second major wave of viral DNA synthesis, peaking at 7.5 h postinfection. No such late cytoplasmic DNA synthesis occurred in the untreated control. Inhibition of protein synthesis by cycloheximide, on the other hand, stimulated cytoplasmic viral DNA synthesis during the first 3.5 h.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D007372:Interferons; D003513:Cycloheximide