Supravital observation of in vitro basophils in immunological reactions.
The migration velocity and morphology of basophils in vitro were examined after the addition of anti-immunoglobulin (anti-IgE or anti-IgG). In atopic asthma patients with high serum IgE levels (more than 1000 i.u./ml) basophils showed increased migration velocity and showed pear-shaped and palmate processes after the addition of anti-IgE, but on the addition of anti-IgG, these basophils did not show increased migration velocity or morphological changes. In intractable asthma patients with low serum IgE levels, these changes occurred with anti-IgG but not with anti-IgE. These in vitro findings suggest a basophil reactivity to anti-IgE and anti-IgG in individual patients and support our previous differentiation of asthma patients according to basophil reaction.
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001491:Basophils / Q000276:immunology*; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008857:Microscopy, Interference; D008858:Microscopy, Phase-Contrast; D013194:Staining and Labeling
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007073:Immunoglobulin E