Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG.
Eggs from immunized chickens may provide a convenient and inexpensive source of antibodies. We describe two simple and efficient methods for purification of IgG from yolk. The antibody is rendered useful for most currently employed immunological techniques. Amounts of antibody corresponding to almost half a litre of antiserum may be recovered from a chicken in one month.
D000818:Animals; D000906:Antibodies; D002645:Chickens; D003429:Cross Reactions; D003911:Dextrans / Q000494:pharmacology; D004530:Egg Yolk; D004531:Eggs; D005260:Female; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007158:Immunologic Techniques; D011817:Rabbits; D011863:Radioimmunoassay
D000906:Antibodies; D003911:Dextrans; D007074:Immunoglobulin G