Biochemical characterization of avian T lymphocyte-specific antigens.
Sera from rabbits injected with quail thymocytes were absorbed on quail bursal and liver cells. The absorbed sera reacted with avian T but not B lymphocytes in an immunofluorescence assay. Material precipitated by the anti-T antisera from lysates of radioiodinated chicken or quail thymocytes was analyzed by one- and two-dimensional electrophoresis. Of two anti-T cell antisera studied in detail, one reacted mainly with a protein of an apparent molecular weight of 45 000 - 55 000, and the other with a protein of an apparent molecular weight 65 000 - 70 000. These proteins may be homologues of previously described mammalian T lymphocytes-specific antigens.
D000818:Animals; D000954:Antigens, Surface; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002060:Bursa of Fabricius / Q000276:immunology; D002417:Cattle; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002645:Chickens / Q000097:blood; D003370:Coturnix; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D000939:Epitopes