title: Liver chromatin fractions in Mus and Akodon. The concept of constitutive heterochromatin.
abstract: The liver chromatin from Mus musculus and Akodon molinae was separated in 8 fractions by differential centrifugation. Like fractions from both species showed approximately similar contents of DNA, equivalent ratios of histone to non-histone proteins, corresponding template activities and equal amounts of positive C-banded material. On the other hand, heavy chromatin fractions of Mus were highly enriched in satellite DNA whereas no satellite DNA was found in Akodon chromatin. Heavy chromatin fractions isolated by differential sedimentation have been usually homologued with the constitutive heterochromatin. The properties of the constitutive chromatin are discussed and the validity of the foregoing concept is challenged. It is proposed to define the constitutive heterochromatin as those chromatin regions comprising highly repeated DNA sequences clustered in restricted areas of chromosomes and not transcribed (satellite DNA).
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis / Q000302:isolation & purification*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004247:DNA / Q000032:analysis; D004276:DNA, Satellite / Q000032:analysis; D006570:Heterochromatin / Q000032:analysis*; D006657:Histones / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008297:Male; D051379:Mice / Q000235:genetics*; D012313:RNA / Q000096:biosynthesis; D012377:Rodentia / Q000235:genetics*; D013698:Templates, Genetic
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D006570:Heterochromatin; D006657:Histones; D012313:RNA; D004247:DNA