[Growth of tumour-cell colonies from human solid tumours (author's transl)].
In-vitro growth of tumour-cell colonies of various human solid carcinomas was investigated. Cell-colony formation in-vitro was achieved from single cells in 46 of 81 tumour specimens. The proportion of tumour-cell colonies per number of cultured tumour cells ("cloning efficiency") thus was 1-8 per 1000. Tumour-cell colonies of various carcinomas differed in their morphological appearance, with characteristic cytological in-vitro. Growth of tumour-cell colonies opens up the possibility of performing cytostatic drug sensitivity tests on defined tumour-cell populations of a given individual tumour. Under the influence of various cytostatic drugs tumour-cell colony numbers could be reduced by more than 30% in 27 of 46 tested carcinomas compared with untreated controls.
D002330:Carmustine / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D002999:Clone Cells; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology; D008722:Methods; D008727:Methotrexate / Q000494:pharmacology; D009369:Neoplasms / Q000473:pathology*
D004317:Doxorubicin; D007372:Interferons; D002945:Cisplatin; D005472:Fluorouracil; D002330:Carmustine; D008727:Methotrexate
Wachstum von Tumorzellkolonien bei menschlichen soliden Tumoren.