Evidence that the serological determinant of H-Y antigen is carbohydrate.
The histocompatibility-Y (H-Y) antigen is a minor histocompatibility antigen which has been detected on cell surfaces from the heterogametic sexes of mammalian, bird, amphibian, teleost and invertebrate species. H-Y is thought to be a male-determining substance in mammals because of its almost perfect correlation with maleness among a variety of mammalian species. To characterize the molecular determinant responsible for H-Y specific serological activity, H-Y positive immunoabsorbent cells were first subjected to various treatments which alter protein or carbohydrate structure and then tested for their ability to absorb H-Y antisera. We present here evidence that the serological determinant of H-Y antigen is carbohydrate.
D000818:Animals; D002241:Carbohydrates / Q000276:immunology*; D000939:Epitopes; D005690:Galactose / Q000276:immunology; D005692:Galactose Oxidase / Q000378:metabolism; D005697:Galactosides / Q000276:immunology; D006182:H-Y Antigen / Q000276:immunology*; D008297:Male; D051379:Mice; D001616:beta-Galactosidase / Q000378:metabolism
D002241:Carbohydrates; D000939:Epitopes; D005697:Galactosides; D006182:H-Y Antigen; D005692:Galactose Oxidase; D001616:beta-Galactosidase; D005690:Galactose