title: Time: a new parameter for kinetic measurements in flow cytometry.
abstract: The measure of time was used as an additional parameter on an existing flow cytometer to study the kinetics of enzyme activities and cell-stain interactions. By correlating all fluorescent signals from single cells with time, the dynamics of a reaction can be followed for several minutes. This advanced application of flow cytometry is easily implemented and can be incorporated into any flow cytometer that has two-parameter analysis capability.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000201:enzymology; D003201:Computers; D006224:Cricetinae; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods; D013194:Staining and Labeling
chemical_list: D004247:DNA; D004950:Esterases