title: The mode of action of nanaomycin A in Gram-positive bacteria.
abstract: The mode of action of nanaomycin A on Gram-positive such as Staphylococcus aureaus, Bacillus cereus and Streptococcus faecalis was investigated. Nanaomycin A inhibited the biosyntheses of protein, DNA, RNA and cell-well peptidoglycan to a similar extent. It increased the oxygenous respiration of S. aureus cells at the minimal inhibitory concentration. The cells preincubated with nanaomycin A showed stimulation of proton influx after addition of N,N'-dicyclo-hexylcazrbodiimide, an inhibitor of Ca++, Mg++-ATPase. Nanaomycin A seems to interfere with the cytoplasmic or to inhibit coupling of oxidative phosphorylation, followed by secondary inhibitory effect on protein, nucleic acids and cell-wall peptidoglycan biosyntheses.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D009285:Naphthoquinones / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D011522:Protons; D012329:RNA, Bacterial / Q000096:biosynthesis; D000095622:Benzoisochromanequinones
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009285:Naphthoquinones; D011522:Protons; D012329:RNA, Bacterial; C008638:nanaomycin A; D000095622:Benzoisochromanequinones