title: Biological functions of t cell lines with specificity for the intracellular bacterium Listeria monocytogenes in vitro and in vivo.
abstract: Peritoneal exudate T lymphocytes from mice immunized with live Listeria monocytogenes were cloned in double-layer soft agar containing heat-killed L. monocytogenes (lower layer) and syngeneic accessory cells (upper layer). Colony-derived T cells were propagated in vitro in the presence of listerial antigen, syngeneic accessory cells, and T cell growth factor. In vitro proliferation, interleukin secretion, and bystander help for B cells of six such T cell lines and several sublines derived from them were found to be antigen dependent and restricted by the H-2IA locus of the major histocompatibility complex. In vivo, these T cell lines conferred delayed-type hypersensitivity to listerial antigen and protection to live L. monocytogenes. It is concluded that different biological functions of acquired antibacterial immunity can be mediated by a single T cell population.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D006183:H-2 Antigens; D007376:Interleukin-2