title: Normal two-dimensional gel electrophoresis of alpha-2-macroglobulin in cystic fibrosis.
abstract: Some studies have suggested that a mutant form of alpha-2-macroglobulin (alpha-2-M) could be the primary defect in cystic fibrosis (CF). To test for the presence of charge change amino acid substitutions, alpha-2-M was examined by two-dimensional gel electrophoresis following complete denaturation of the proteins. The pattern of nine charge isomers observed was the same in homozygous, heterozygous, and normal individuals.
mesh_terms: D001797:Blood Protein Electrophoresis; D003550:Cystic Fibrosis / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D000511:alpha-Macroglobulins / Q000235:genetics*
chemical_list: D000511:alpha-Macroglobulins