title: Alterations of transcription during heat shock of Saccharomyces cerevisiae.
abstract: The pattern of polyadenylated RNA of the yeast Saccharomyces cerevisiae changes dramatically upon heat shock. By in vitro translation, we have demonstrated that a 2.9-kilobase heat shock RNA encodes the Mr = 90,000 yeast heat shock protein. Heat shock-responsive genes have been isolated by differential plaque filter hybridization of a recombinant library of yeast DNA inserted in the vector lambda Charon 4. The putative yeast gene products of a number of these recombinants molecules has been determined by in vitro translation of hybrid-selected RNA. We have used one of these hybrid phages (lambda Yhsil) to demonstrate that the heat shock-induced alteration in the level of the 2.9-kilobase polyadenylated RNA which encodes the Mr = 90,000 yeast heat shock protein is regulated at the level of transcription.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006360:Heat-Shock Proteins; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D006360:Heat-Shock Proteins; D011506:Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes