Effects of insulin on messenger RNA activities in rat liver.
Liver poly(A) RNA, isolated from adrenalectomized rats after insulin treatment, was translated in a nuclease-treated lysate of rabbit reticulocytes and quantitated for both total activity and the capacity to synthesize the insulin-inducible enzyme tyrosine aminotransferase. Analysis of the translated products from poly(A) RNA isolated 1 h after insulin treatment showed a 2.7-fold increase in activity of tyrosine aminotransferase mRNA. During the same interval, the capacity of poly(A) RNA to direct the synthesis of total protein in lysates also changed, showing a 30 to 40% increase in translational activity/unit of RNA. Increased translatability was apparent in all fractions of poly(A) RNA separated by centrifugation on sucrose gradients. Insulin thus appears to mediate a generalized change in mRNAs leading to increased capacity for translation; induction of tyrosine aminotransferase may reflect sensitivity to effect of the hormone.
D000315:Adrenalectomy; D000818:Animals; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis
D007328:Insulin; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D014444:Tyrosine Transaminase