Major histocompatibility complex-restricted H-Y-specific antibodies and cytotoxic T lymphocytes may recognize different self determinants.
Previous studies have shown that influenza virus-immune cytotoxic T lymphocytes can recognize virus in conjunction with self HLA-A2 antigens. Nevertheless, the virus-infected target cells from one HLA-A2-positive male donor (designated M7) could not be lysed by the virus-immune cytotoxic lymphocytes from any HLA-A2-matched unrelated donors. Although extensive serological analyses showed no difference between the HLA-A2 antigens of donor M7 and other HLA-A2-positive donors, isoelectric focusing of the HLA-A2 molecule from donor M7 revealed a clear difference in the heavy polypeptide chains when compared with the HLA-A2 molecules of other donors. The present study demonstrates that the HLA-A2-restricted anti-H-Y cytotoxic T lymphocytes obtained from a female aplastic anaemia patient fail to lyse the male M7 target cells, whereas the HLA-A2-restricted anti-H-Y antibodies from the same patient react with the cells of donor M7. These results suggest that: (a) HLA-A2-restricted anti-H-Y antibodies can recognize self determinants on the HLA-A2 molecule that are distinct from those that are recognized by HLA-A2-restricted anti-H-Y cytotoxic T cells; and (b) HLA-restricted T and B cells may use different receptor repertoires for the recognition of foreign antigens such as H-Y.
D000741:Anemia, Aplastic / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000235:genetics / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics; D005260:Female; D006182:H-Y Antigen / Q000235:genetics* / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000235:genetics* / Q000276:immunology; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D000939:Epitopes; D006182:H-Y Antigen; D006680:HLA Antigens; D007518:Isoantibodies