[Interferon production in suspensions of swine and human leukocytes on media containing aminopeptide, hemohydrolysate and bovine amniotic fluid].
Production of different kinds of interferon in imported media (medium 199, Eagle's medium) greatly impedes the organization of large-scale interferon production in this country. The search for national substitutes of these media is important therefore. This paper deals with a comparative study of the use of national aminopeptide and hemohydrolysate preparations for interferon production. It was shown that substitution of serum with BAF in preparations of human and swine interferon increased their activity 2- and 4-fold, respectively, and the amount of protein was reduced 10-fold. Specimens of interferons produced with the addition of AP were less effective because the amount of protein increased 4-20-fold while the antiviral activity increased insignificantly. The most effective were interferon preparations obtained with hemohydrolysate medium. Their activity being similar, the amount of protein in interferon samples increased 11/2-2-fold only. Thus, aminopeptide and hemohydrolysate can effectively substitute medium 199 for production of leukocyte human and swine interferon.
D000653:Amniotic Fluid; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000494:pharmacology; D013552:Swine / Q000097:blood
D003470:Culture Media; D010455:Peptides; D011492:Protein Hydrolysates; D007372:Interferons
Poluchenie interferona v suspenzii svinykh i chelovecheskikh leĭkotsitov na sredakh s aminopeptidom, gemogidrolizatom i amnioticheskoĭ zhidkost'iu korov.