title: RNA-dependent DNA polymerase activity in ocular granulocytic sarcoma associated to acute myelomonocytic leukemia in Turkish children. Biochemical and immunological characterization of the enzyme.
abstract: The present study describes the separation and purification of a reverse transciptiase from an orbital tumor of a patient with acute myelomonocytic leukemia. Specific reaction conditions with respect to ionic requirements and template-primers are reported. The purified enzyme was able to transcribe (rA)n . (dT)12, (rC)n . (dG)12, (OMeC)n . (dg)12 and the 70 S RNA from R(Mu)LV. Serological studies that the reverase transcriptase is antigenically related to reverse transcriptase from the type C woolly monkey virus-gibbon ape leukemia virus group.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002648:Child; D000882:Haplorhini; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000150:complications / Q000201:enzymology*; D009918:Orbital Neoplasms / Q000201:enzymology* / Q000556:secondary; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D012190:Retroviridae / Q000276:immunology; D012842:Sarcoma Virus, Woolly Monkey / Q000276:immunology; D014158:Transcription, Genetic; D014421:Turkey
chemical_list: D000914:Antibodies, Viral; D012194:RNA-Directed DNA Polymerase