In vitro studies of nuclear volume of rat adrenocortical cells: lace of ACTH effect.
The effect of ACTH on nuclear volume of adrenocortical cells in the zona fasciculata of rat adrenal cortex was examined in vitro. Sections of adrenal gland were incubated for 60 or 90 min in Krebs-Ringer's solution with 1% glucose in the presence of ACTH, actinomycin D, cycloheximide and aminoglutethimide. ACTH, despite its clear effect in stimulating steroidogenesis, did not exert a direct effect on the nuclear volume of cells studied. This phenomenon is not dependent upon the stimulation of steroidogenesis, since aminoglutethimide does not influence the nuclear volume of adrenocortical cells studied; rather, ACTH in the presence of aminoglutethimide leads to a decrease in their volume. Actinomycin D does not influence nuclear volume while after incubation with cycloheximide nuclei were larger than the control. The presence of ACTH did not alter this effect. These results indicate no relationship between the degree of corticosterone output and nuclear volume in rat adrenocortical cells of the zona fasciculata in vitro.
D000302:Adrenal Cortex / Q000187:drug effects / Q000648:ultrastructure*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000616:Aminoglutethimide / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D003345:Corticosterone / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D051381:Rats
D000616:Aminoglutethimide; D003609:Dactinomycin; D000324:Adrenocorticotropic Hormone; D003513:Cycloheximide; D003345:Corticosterone