title: A low molecular weight nuclear RNA from HeLa cells inhibits exogenous messenger RNA-dependent protein synthesis.
abstract: A low molecular weight RNA of nuclear origin from adenovirus-infected HeLa cells inhibited rabbit reticulocyte polyribosomal RNA-dependent cell-free protein synthesis by reducing th formation of 80 S ribosome from 40 S-mRNA complex and 60 S subunits. This inhibitor did not hybridize with intact or HindIII-digested fragments of the viral DNA. Therefore it appears to be a 5.5 S RNA of cellular origin, which binds significantly with authentic IF-M2B from rabbit reticulocytes. In all these characteristics the inhibitor appears identical to a similar 5.5 S inhibitor RNA from non-infected HeLa cells.
mesh_terms: D000256:Adenoviridae; D000818:Animals; D002474:Cell-Free System; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009693:Nucleic Acid Hybridization; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012313:RNA; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII