[The antigen structure of human serum transferrin].
By means of enzymatic fragmentation the transferrin molecule was definitely divided up into fragments which were investigated immunochemically after their column-chromatographic isolation. Papain, pepsin, ficin, and bromelin were used as proteolytic enzymes. Immunoelectrophoresis and various procedures of immunodiffusion were used for characterizing the antigen structure of transferrin fragments in an immunochemical way. The antisera required were obtained by immunizing animals (horses, sheep, goats, rabbits) according to different procedures. It was possible to divide up transferrin into 4 defined fragments, with the antigen structure being divided into three separate groups (A, B, C). The antigen structure of human transferrin consists of at least 6 different antigen determinants designated as a1, b1, b2, b3, b4, and c1 in accordance with their being included in the corresponding antigen determinant group. An interior antigen determinant (bi) could be proved in the bromelin transferrin fragment II. An attempt was made to develop a model of transferrin molecule on the basis of the findings achieved.
D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides / Q000276:immunology; D013329:Structure-Activity Relationship; D014168:Transferrin / Q000276:immunology*
D000939:Epitopes; D010446:Peptide Fragments; D010455:Peptides; D014168:Transferrin
Zur Antigenstruktur des humanen Serumtransferrins.