Timing of swarmer cell cycle morphogenesis and macromolecular synthesis by Hyphomicrobium neptunium in synchronous culture.
The swarmer cycle of Hyphomicrobium neptunium consists of a temporal sequence of discrete developmental events. To time morphogenesis and to investigate modulations in macromolecular synthesis, we attempted methods for synchronous culture. During synchrony, swarmer maturation occurred over 32%, hyphal growth occurred over 36%, and bud maturation occurred over 32% of the time required to complete the swarmer cycle. Daughter cells were released after 265 min. Deoxyribonucleic acid replication was discontinuous, having a G1 period of approximately 180 min. In addition, ribonucleic acid and protein syntheses were depressed during the earlier phases of development.
D001419:Bacteria / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002453:Cell Cycle; D004269:DNA, Bacterial / Q000096:biosynthesis; D007700:Kinetics; D009024:Morphogenesis; D012329:RNA, Bacterial / Q000096:biosynthesis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial