Effect of peptides of bovine myelin basic protein on dermal fibroblasts.
Bovine basic protein (BP) was digested with purified bovine brain cathepsin D to produce well defined BP derived peptides 1-42, 43-88, 43-169, and 89-169. BP and these BP peptides were tested for their effects on cultured human dermal fibroblasts using a concentration range of 0.01-1,000 ng/ml. No effect was found on cellular proliferation, and neither total protein nor collagen synthesis was altered in the presence of these substances. Although preparations of brain which contain a fibroblast-growth factor also contain BP peptides, these results indicate that the purified BP peptides studied have no detectable biological effect on the growth of human dermal fibroblasts.
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D004676:Myelin Basic Protein / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012867:Skin / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D004676:Myelin Basic Protein; D010446:Peptide Fragments; D003094:Collagen; D004247:DNA; D007930:Leucine; D013936:Thymidine