[SDS-polyacrylamide gel electrophoretic analysis of serum protein in maternal and cord serum (author's transl)].
In order to study the changes of serum protein in maternal and cord serum, sodium dodecylsulfate-polyacrylamide gel electrophoresis was applied to the fraction of serum protein. The results obtained are as follows; 1. Using this method, fifteen bands were detectable in the serum of normal non-pregnant women. 2. Fractions 5 (M.W.=18.5 X 10(4)) and 19 (M.W.+2.3 X 10(4)) changed during the course of normal pregnancies. Values of fraction 5 increased from nonpregnant values of 1.8 +/- 0.5% to 3.0 +/- 0.8% during the first trimester, and decreased to the non-pregnant level after 25 weeks of gestation. At 21 approximate 24 weeks of gestation, values of fraction 10 approximate 11 rose to plateau of 13.5 +/- 1.0%, which was maintained until delivery, compared with 10.4 +/- 2.0% in nonpregnant women. Values of fraction 19 increased from non-pregnant values of 2.3 +/- 0.7% to 3.7 +/- 1.1% at 25 approximate 28 weeks of gestation, and, thereafter, remained unchanged until delivery. However, there was a decrease to 1.6 +/- 0.9% at one month after delivery. 3. In cord serum, values of fraction 10 approximate 11 were significantly lower than in maternal serum. Fraction 14 (M.W.=4.5 X 10(4)), 15(M.W.=3.8 X 10(4)), and 22 (M.W.=1.6 X 10(4)), which were not detectable in normal adult serum, were detectable, but fractions 1 (M.W.=28 X 10(4)), 2 (M.W.=26 X 10(4)), 3 (M.W.=25 X 10(4)) and 4 (M.W.=21 X 10(4)) were undetectable in the cord serum.
D001797:Blood Protein Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005312:Fetal Blood / Q000032:analysis*; D006801:Humans; D049590:Postpartum Period; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D000511:alpha-Macroglobulins / Q000032:analysis
D000511:alpha-Macroglobulins