Antigen-induced and non-antigen-induced histamine release from rat mast cells sensitized with mouse antiserum.
Marked IgE-mediated histamine release from rat mast cells sensitized in vitro with mouse antiserum occurs in the presence of added Ca++ and phosphatidylserine (PS), although a considerable degree of antigen-induced histamine release which may utilize intracellular or cell-bound calcium is also observed. The decay in the responsiveness to Ca++ of the sensitized cells stimulated by antigen in Ca++-free medium in the presence of PS is relatively slow, and maximum release is produced by Ca++ added 1 min after antigen. Histamine release also occurs when Ca++ is added after PS in the absence of antigen to the sensitized cells suspended in Ca++-free medium. Unlike the antigen-induced release, the intensity of this non-antigen-induced release varies depending on both mast-cell and antiserum pools. A heat-labile factor(s), which is different from antigen-specific IgE antibody and is also contained in normal mouse serum, is involved in this reaction. In the antigen-nondependent (PS + Ca++)-induced release, no decay in the responsiveness to Ca++ is observed after PS addition. Both the antigen-induced and non-antigen-induced release are completed fairly rapidly and are dependent of temperature, pH and energy.
D019297:2,4-Dinitrophenol; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002118:Calcium / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010718:Phosphatidylserines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature
D004140:Dinitrophenols; D010718:Phosphatidylserines; D007073:Immunoglobulin E; D010047:Ovalbumin; D019297:2,4-Dinitrophenol; D002118:Calcium