Direct radioimmunoassay for the detection of IgM antibodies against Mycoplasma pneumoniae.
A direct solid-phase radioimmunoassay is described for detecting IgM-class antibodies against Mycoplasma pneumoniae. The assay achieves a prelminary separation of IgM from other serum proteins by immunoadsorption to anti-IgM-coated wells in microtitre plates. The IgM is then tested for antigen specificity by measuring its ability to bind radiolabelled M. pneunoiae. Positive results are confirmed by retesting sera after treatment with 2-metcaptoethanol. The assay is specific for IgM-class antibodies and specific for M. pneumoniae. It is not affected by competitive inhibition from IgG and avoids the use of density gradient centrifugation or gel filtration to separate IgM from other immunoglobulins. It is sensitive, reproducible, rapid, simple and requires very little serum.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001665:Binding Sites; D000939:Epitopes; D006041:Goats; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M; D008623:Mercaptoethanol / Q000494:pharmacology; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D012756:Sheep; D013552:Swine; D013696:Temperature; D013997:Time Factors
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008623:Mercaptoethanol