[Reliability of DNA measurement after simultaneous staining of DNA and RNA by acridine-orange for flow-cytophotometry (author's transl)].
The reliability of DNA cell content measurement by flow cytophotometry was assessed after simultaneous staining of DNA and RNA by acridine-orange. The methods of staining allowing the lowest CV were determined. The specificity of this staining for DNA appeared satisfactory. When the distribution in the various phases of the cell cycle was computerized, a good correlation was found for the S phase with the 3H-thymidine labeling index. The interest of this method for prognosis and monitoring of acute leukemia is underlined.
D000165:Acridine Orange; D004247:DNA / Q000032:analysis*; D006801:Humans; D010783:Photometry; D012313:RNA / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods
D012313:RNA; D004247:DNA; D000165:Acridine Orange
Coloration simultanée de l'ADN et de l'ARN par l'acridine orange pour cytophotometrie en flux liquide. Etude de la fiabilité de la mesure de l'ADN.