[Effect of glutamic acid content of the diet on isotope-labeled glutamic acid catabolism in rats. 1. The course of 14CO excretion following intragastric administration of 14C-glutamic acid].
Male rats received in 8 groups of 10 animals each for a period of 7 days 7 synthetic diets and one semisynthetic diet on maintenance requirement level. A L-amino acid mixture corresponding to the pattern of egg protein without glutamic acid was the protein source of the synthetic diets. Glutamic acid was supplemented successively from 0 to 58 mol-% of the total amino acid content. The crude protein source of diet 8 was whole egg powder. On the 8th day of experiment 5 animals per group were labelled by intragastric infusion (i.g.) with 14C-U-glutamic acid. During the following 24 hours the excretion of CO2 and 14CO2 was measured. Throughout the experimental feeding body weight was relative constant, however, when the synthetic diets were fed it was necessary to increase the daily amount of energy from 460 to 480 kJ/kg0,67. The relative 14CO2-excretion within 24 hours was 68-75% of the dose. However, the main part of the amount of radioactivity excreted during 24 hours was found after 4 to 6 hours already. Exponential functions calculated from the data of cumulative 14CO2-excretion suggest the existence of a fast process of 14CO2-formation directly from 14C-glutamic acid, reaching a plateau within 2 hours and a slow process of oxidation of intermediates of glutamic acid metabolism, causing a continued 14CO2-formation even after 24 hours. The oxidation of 14C-glutamic acid to CO2 decreased 2 to 14 hours after i.g. labelling if the glutamic acid content of the diet increased. The same was found for the specific radioactivity of 14CO2. A storage of intermediates of glutamic acid before degradation was assumed.
D000818:Animals; D000830:Animals, Laboratory; D001835:Body Weight; D002245:Carbon Dioxide / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004734:Energy Metabolism; D005971:Glutamates / Q000008:administration & dosage / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D051381:Rats / Q000378:metabolism*
D002250:Carbon Radioisotopes; D005971:Glutamates; D002245:Carbon Dioxide; D018698:Glutamic Acid
Der Einfluss des Glutaminsäuregehaltes der Diät auf die Katabolisierung von isotopenmarkierter Glutaminsäure bei Ratten. 1 Mitteilung. Verlauf der 14CO-Ausscheidung nach intragastraler 14C-Glutaminsäureverabreichung.