title: Localization of proteoglycan monomer and link protein in the matrix of bovine articular cartilage: An immunohistochemical study.
abstract: Using monospecific antisera and immunofluorescence microscopy, proteoglycan monomer (PG), and link proteins were demonstrated throughout the extracellular matrix of bovine articular cartilage. A narrow band of strong pericellular staining was usually observed for both molecules, indicating a pericellular concentration of proteoglycan monomer: this conclusion was supported by dye-binding studies. Whereas PG was evenly distributed throughout the remaining matrix, more link protein was detectable in interterritorial sites in middle and deep zones. Well-defined zones of weaker territorial staining for link protein stained strongest for chondroitin sulfate. Trypsin treatment of cartilage resulted in a loss of most of the PG staining, but some selective retention of link protein, particularly around chondrocytes in the superficial zone at and near the articular surface. This residual staining was largely removed if sections were fixed after chondroitinase treatment. After extraction of cartilage with 4M guanidine hydrochloride, only PG remained and this was concentrated in the superficial zone. These observations are shown to support the concept of aggregation of PG and link protein with hyaluronic acid (HA) in cartilage matrix, and the binding of PG and link protein to HA, which is attached to the chondrocyte surface. Culture of cartilage depleted of PG and link protein by trypsin demonstrated that individual chondrocytes can secrete both PG and link proteins and that the organization of cartilage matrix can be regenerated in part over a period of 4 days.
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000276:immunology*; D002417:Cattle; D006146:Guanidines / Q000494:pharmacology; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011506:Proteins / Q000302:isolation & purification*; D011509:Proteoglycans / Q000302:isolation & purification*; D013194:Staining and Labeling; D014357:Trypsin / Q000494:pharmacology
chemical_list: D006146:Guanidines; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011506:Proteins; D011509:Proteoglycans; D014357:Trypsin