A membrane filtration method for estimating the number of viable bacteria in solutions containing antibiotic.
The method involves filtration of dilutions of the mixture through a membrane filter followed by replication on ox blood agar and enumeration of organisms. This method was compared with the conventional drop count method, and a significant correlation was found (r = 0.985). When solutions containing antibiotic were used, the membrane filtration method gave a more reliable estimate of the viable count at the time of sampling.
D000818:Animals; D002417:Cattle; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D003023:Cloxacillin / Q000494:pharmacology*; D005260:Female; D005374:Filtration / Q000379:methods; D008414:Mastitis, Bovine / Q000382:microbiology; D013211:Staphylococcus aureus / Q000254:growth & development*
D003023:Cloxacillin