In vitro degradation of substance P: new rapid assay and distribution in rat brain.
An ion exchange batchwise procedure was developed for the estimation of substance P degradation by rat brain fractions using 3H[Nle11]substance P as tracer. With this very sensitive and rapid method an especially high degradative activity was found among others in the microsomal fractions of the striatum.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000031:analogs & derivatives* / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013373:Substance P; C015874:substance P, NleNH2(11)-