title: Altered pharmacological properties of liposome-associated human interferon-alpha.
abstract: Human interferon-alpha was associated in different ways with positively (stearylamine) and negatively (phosphatidylserine) charged phosphatidylcholine multilamellar vesicles, depending on the presence or absence of a cholesterol component. Inclusion of cholesterol resulted in interferon that was significantly (P = 0.0001) more deeply internalized within the liposomes, such that detergent disruption was necessary before most of the interferon activity was expressed. Interferon was stably associated with stearylamine-containing liposomes, both with and without a cholesterol component. However, inclusion of cholesterol in the phosphatidylserine-containing liposomes was necessary for stable association of the interferon for more than 2 days at 4 degrees C or for more than 24 h at 37 degrees C. After intramuscular injection into mice, liposome-associated interferon in reverse-phase evaporation vesicles was retained at the local site of injection significantly longer than free interferon. Even 3 days after intramuscular injection, stearylamine-containing liposomes with or without cholesterol resulted in local interferon levels that were comparable to the peak levels obtained 2 to 4 h after free interferon was injected. In contrast, free interferon was not detectable in the local muscles 24 h after injection of 10(4.6) U. Liposomes containing phosphatidylserine and cholesterol resulted in intermediate levels of local interferon retention; without a cholesterol component, phosphatidylserine-containing liposomes resulted in no increased local interferon retention compared with the results when free interferon was injected.
mesh_terms: D000818:Animals; D002784:Cholesterol; D007372:Interferons / Q000008:administration & dosage*; D008081:Liposomes; D008657:Metabolic Clearance Rate; D051379:Mice; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D013329:Structure-Activity Relationship
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D007372:Interferons; D002784:Cholesterol