Molecular heterogeneity of D-end products detected by anti-H-2.28 sera. I. A. molecule similar to Qa-2, detected in the BALB/cBy but not in the BALB/c-H-2dm2 mutant.
Immunoprecipitation of NP-40 lysates of 125I-labeled lymph-node cells with different anti-H-2 sera and with anti-Qa-2 serum has shown that the BALB/cByA strain (H-2d, Qa-2-negative) expresses, besides H-2Ld, another molecule that is not detectable in the BALB/c-H-2dm2 strain. Electrophoresis in SDS polyacrylamide gels indicated that this molecule, provisionally designated Lq, has an apparent molecular weight of 41000 daltons, in contrast to approximately 49000 daltons for H-2Kd and H-2Ld, and 47000 daltons for H-2Dd molecules. The anti-Qa-2 serum precipitated from the Qa-2-positive strains BALB/cHeA but not from the Qa-2-negative strains BALB/cByA and BALB/c-H-2dm2 a protein that gave a very strong band corresponding to the molecular weight 41000 daltons in the gel electrophoresis. The biochemical characteristics of the Lq molecule are thus more similar to those of Qa-2 than of H-2 antigens.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D008970:Molecular Weight; D009154:Mutation; D013045:Species Specificity
D000954:Antigens, Surface; D000939:Epitopes; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies; C032907:Q surface antigens