In vitro studies on the mechanism of action of a new antiallergic, Ro 21-7634.
The effects of Ro 21-7634 and disodium cromoglycate (cromoglycate) on the in vitro release of mediators of anaphylaxis from rat peritoneal cells and guinea pig lung tissue were compared. Ro 21-7634 was 25 fold more potent than cromoglycate as an inhibitor of antigen-induced histamine release from passively sensitized (IgE) rat peritoneal cells. Ro 21-7634 was also the more potent inhibitor of both compound 48/80- and concanavalin A-induced histamine release from rat peritoneal cells. The two drugs shared the common properties of producing the same maximal level of inhibition in each of the above releasing systems and exhibiting a time and concentration dependent loss of inhibitory activity when added to the cells prior to the releasing agent. Neither drug inhibited ionophore A23187-or ionophore X537A-induced histamine release from these cells. Ro 21-7634 inhibited antigen-induced (IgG1) histamine and SRS-A release from actively sensitized guinea pig lung fragments, whereas cromoglycate did not. The results indicate that Ro 21-7634 and cromoglycate act through a common mechanism to inhibit allergic mediator release and that Ro 21-7634 is the more potent inhibitor.
D000818:Animals; D003208:Concanavalin A / Q000037:antagonists & inhibitors; D004205:Cromolyn Sodium / Q000494:pharmacology*; D006633:Histamine Antagonists; D006636:Histamine Release / Q000187:drug effects; D008168:Lung / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D051381:Rats; D013189:SRS-A / Q000037:antagonists & inhibitors*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006633:Histamine Antagonists; D013189:SRS-A; D003208:Concanavalin A; D003189:p-Methoxy-N-methylphenethylamine; D004205:Cromolyn Sodium