Molecular cloning of a cDNA sequence encoding a trophectoderm-specific marker during mouse blastocyst formation.
A cDNA clone has been isolated from a trophoblastoma cDNA library. The mRNA complementary to this sequence directs the in vitro synthesis of proteins. The two-dimensional electrophoretic pattern of migration of these proteins is superposable to that of the trophoblastoma intermediate filament proteins recognized by monoclonal antibody (mAb) TROMA-1. This mAb had previously been shown to label trophectoderm cells but not inner cell mass cells. With a sensitive binding assay (ultrasensitive enzymatic radioimmunoassay), these in vitro synthesized proteins were recognized by mAb TROMA-1. The proteins are immunoprecipitated by an antiserum directed against trophoblastoma intermediate filament proteins and by a serum directed against a major cytoskeletal protein found in murine extraembryonic endodermal cell lines (Endo-A) [Oshima R. G. (1981) J. Biol. Chem. 256, 8124-8133]. The cDNA sequence detects specific mRNA(s) migrating with 18S ribosomal RNA in trophoblastoma but not in embryonal carcinoma cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D001755:Blastocyst / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D013724:Teratoma
D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D012333:RNA, Messenger; D012313:RNA; D004247:DNA