[Method of determination of proteins with Coomassie brilliant blue G 250. I. General characteristics and comparative analysis with the biuret method and Lowry's method].
Bradford (1977) described a new sensitive easy rapid method for protein assay, depending on colour change of the stain Coomassie Brilliant Blue G 250. In this study Bradford's method was compared with the Lowry et al. 91951) and biuret methods. Coomassie method appears to be as sensitive as Lowry, as simple as biuret. Another advantage is a very short time of performing.
D008722:Methods; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013194:Staining and Labeling
D011506:Proteins; D012710:Serum Albumin, Bovine
Metodo di dosaggio delle proteine con Coomassie brilliant blue G 250. Nota I. Caratteristiche generali e analisi comparativa con i metodi del biureto e di Lowry.