title: [Kinetics of DNA-dependent synthesis of RNA: correlation between abortive and productive initiations].
abstract: A general equation of abortive initiation kinetics is inferred at its coupling to a productive initiation. It describes the abortive initiation dependence upon time and concentrations of the initiating nucleotide and the following nucleosidetriphosphate (NTP). The equation analysis revealed the abortive initiation dependence upon NTP's concentration in a characteristic manner possessing a maximum at the time it comes to saturation. The kinetic constants were estimated experimentally from the plot when the extent of pApU synthesis was plotted first against time in presence of a complete set of NTP's and second, against AMP and UTP concentration in the absence of the other three NTPs. The estimated values of the constants were introduced into the equation to analyze the dependence of pApU synthesis upon NTPs concentration ([ATP]=0.2 [UTP]=[GTP]=[CTP]=[NTP]). The theoretically calculated curve was compared to the experimentally obtained. Good coincidence of these curves supports the correctness of the general equation. The general equation of abortive initiation gives possibility to infer an equation of transcription involving the initiation and the elongation studies.
mesh_terms: D004247:DNA; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D012313:RNA / Q000235:genetics*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012265:Ribonucleotides; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
chemical_list: D012329:RNA, Bacterial; D012265:Ribonucleotides; D012313:RNA; D004247:DNA
vernacular_title: Kinetika DNK-zavisimogo sinteza RNK: sootnoshenie reaktsiĭ abortivnoĭ i produktivnoĭ initsiatsii.