Stability of avian leukosis virus type-specific antigen in the cell after fixation in periodate-lysine-paraformaldehyde.
Stability of avian leukosis virus type-specific antigen in chicken embryo fibroblasts after fixation in periodate-lysine-paraformaldehyde was tested by the indirect immunoperoxidase method. Antigen that had been steeped in 0.015M Tris-HCl saline buffer solution containing 20% bovine serum and 30% glycerin was preserved at -20 degrees C, 4 degrees C and room temperature without loss of its antigenicity as long as 16 weeks. It was also stable against heat treatment at 56 degrees C, acid treatment and ethyl ether treatment.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D001354:Avian Leukosis Virus / Q000032:analysis* / Q000276:immunology; D002642:Chick Embryo; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology; D005557:Formaldehyde; D007124:Immunoenzyme Techniques; D008239:Lysine; D010504:Periodic Acid; D011108:Polymers
D000956:Antigens, Viral; D000939:Epitopes; D011108:Polymers; D010504:Periodic Acid; D005557:Formaldehyde; C009288:metaperiodate; D008239:Lysine; C003043:paraform