[Expression of the liver mitochondrial genome in diabetes].
In vivo labelled liver mitochondrial RNA of normal and alloxan-diabetic rats are hybridized with mitochondrial DNA with similar efficiency. The liver mitochondrial RNAs have equal ability to competition with N-specific complementary RNA for hybridization on mitochondrial DNA and its fragments cloned in the composition of bacterial plasmids and characterized by a definite set of genes. Differences between concurrent ability of the liver mitochondrial RNAs of normal and alloxan-diabetic animals were not observed. There were no electrophoretic distinctions in mitochondrial RNAs in both groups of animals during RNA electrophoresis in agaroso-acrylamide gel, as well. The results obtained indicate a close set of RNA molecules in the liver mitochondria of normal and diabetic rats. Thus, a previously observed RNA biosynthesis decrease in the liver mitochondria in diabetes is caused not by a selected inhibition of definite gene expression but by a gradual fall of transcription of all the mitochondrial genome parts.
D000496:Alloxan; D000818:Animals; D004247:DNA / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D005786:Gene Expression Regulation; D006824:Hybridization, Genetic; D008297:Male; D008930:Mitochondria, Liver; D012313:RNA / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic
D012313:RNA; D000496:Alloxan; D004247:DNA
Ekspressiia mitokhondrial'nogo genoma v pecheni pri diabete.