Fecal intestinal alkaline phosphatase: a parameter for toxic damage of the small intestinal mucosa.
An immunoprecipitation procedure is presented for the measurement of rat intestinal alkaline phosphatase (I-AP) in feces. Controls exhibit a logarithmic normal distribution of fecal I-AP. Single administration of an agent toxic for small intestinal mucosa like bleomycin or triparanol induces an increase of fecal I-AP on the 1st day and a marked reduction of I-AP activity on the following 3 days. After the 5th day, abnormal high fecal I-AP activities were observed during regeneration. In parallel, morphometry and measurements of I-AP activity in the homogenate of small intestinal mucosa were performed. Rat fecal I-AP excretion proved to be a simple, non-invasive, sensitive marker for toxic damage of the small intestinal mucosa.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D005243:Feces / Q000201:enzymology*; D005260:Female; D007118:Immunoassay; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D051381:Rats; D014308:Triparanol / Q000633:toxicity*
D007527:Isoenzymes; D001761:Bleomycin; D014308:Triparanol; D000469:Alkaline Phosphatase