Purification and characterization of kallikrein from plasma of patients with acute pancreatitis.
1. A kallikrein-like enzyme in plasma of patients with acute pancreatitis was further purified by successive hydroxyapatite/cellulose and Sepharose-4B column chromatography. 2. By these procedures 0.26 mg of purified enzyme with a specific activity of 215 S-2266 chromozyme units/mg of protein was obtained from 10 ml of original plasma. 3. The purified material was homogeneous as ascertained by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and had an apparent molecular weight of 31 000 as measured by gel filtration on Sephadex G-200. 4. It was confirmed immunologically that this enzyme was pancreatic kallikrein, which is distinct from plasma kallikrein, and that it could combine with alpha 2-macroglobulin only in the presence of trypsin.
D000208:Acute Disease; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007610:Kallikreins / Q000097:blood* / Q000302:isolation & purification; D008970:Molecular Weight; D010195:Pancreatitis / Q000201:enzymology*; D014357:Trypsin / Q000494:pharmacology; D000511:alpha-Macroglobulins / Q000378:metabolism
D000511:alpha-Macroglobulins; D007610:Kallikreins; D014357:Trypsin