Separation of epidermal cells by density centrifugation: a new technique for studies on normal and pathological differentiation.
Murine keratinocytes, isolated by flotation trypsinization of skin, can be separated into five groups by centrifugation through Percoll, a colloidal silica gradient. Within each group a good correlation was found between density, plating efficiency, morphological appearance, DNA synthesis, and degree of keratinization/cornification. This method can be applied equally well to fetal, newborn, or adult keratinocytes and should be useful in a variety of studies including isolation of subpopulations of pathological cell types, work on chalones and hyperplastic diseases such as psoriasis, and in vitro transformation studies.
D000818:Animals; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D000078404:Epidermal Cells; D007633:Keratins / Q000032:analysis; D051379:Mice; D011205:Povidone; D012822:Silicon Dioxide; D012867:Skin / Q000166:cytology*
C016039:Percoll; D007633:Keratins; D012822:Silicon Dioxide; D004247:DNA; D011205:Povidone