Effect of leupeptin on the intracellular degradation of asialofetuin in isolated rat hepatocytes.
The effect of the protease inhibitor leupeptin on the intracellular distribution of [14C]-sucrose-asialofetuin in isolated rat hepatocytes was investigated. Leupeptin had no effect on the uptake but reduced the degradation of asialofetuin. Fractionation of hepatocytes by isopycnic centrifugation in sucrose gradients indicated that prolonged treatment with leupeptin inhibited the uptake of asialofetuin into the lysosomes. Therefore, leupeptin inhibits degradation of asialofetuin both by inhibiting intralysosomal proteolysis and transport of endocytosed asialofetuin to the lysosomes.
D000818:Animals; D001212:Asialoglycoproteins; D001692:Biological Transport / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D004705:Endocytosis; D060748:Fetuins; D007976:Leupeptins / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism*
D001212:Asialoglycoproteins; D060748:Fetuins; D007976:Leupeptins; D009842:Oligopeptides; D000509:alpha-Fetoproteins; C017029:asialofetuin