[Localization of virus sequences in heavy nuclear RNA of mouse cells transformed by SV40].
The fraction of poly(A)+-nuclear RNA from SV40 transformed mouse cells contain a high percentage of virus specific sequences. Large primary transcripts containing host and virus specific sequences were analyzed by means of localization of virus sequences on them. For this purpose, the molecules of heavy (> 28S) nuclear poly(A)+-RNA were fragmentated and 3'-poly(A)+-fragments were separated by poly(U)-Sepharose chromatography. These fragments were then hybridized with SV40 DNA. It was shown, that 3'-poly(A)+-fragments of pre-mRNA were enriched in virus specific sequences in comparison with complete molecules. Most of the virus specific sequences in poly(A)+-pre-mRNA, which contain these sequences are localized near the 3'-end of the molecule. Possible schemes of the structure of virus transcripts in transformed cells are discussed.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D002472:Cell Transformation, Viral; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D013539:Simian virus 40; D014158:Transcription, Genetic
D012367:RNA, Viral; D011061:Poly A; D012313:RNA
Izuchenie lokalizatsii virusnykh posledovatel'nosteĭ v tiazheloĭ iadernoĭ RNK kletok myshi, transformirovannykh virusom SV40.