DE-52 and DE-53 cellulose microcarriers. I. Growth of primary and established anchorage-dependent cells.
DEAE cellulose anion exchangers having small ion exchange capacity (0.5 to 2.0 meq/g dry material) were tested for cell attachment kinetics and capacity to support growth anchorage-dependent cells. It was found that cells from established cell lines (BHK and MDCK) can grow to confluency on DEAE cellulose particles having exchange capacity of 1 and 2 meq/g dry materials, DE-52 and DE-53, respectively. On the other hand, chick embryo fibroblasts (primary cells) can grow only on DE-53 particles.
D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D002482:Cellulose / Q000031:analogs & derivatives; D002642:Chick Embryo; D006224:Cricetinae; D003636:DEAE-Cellulose; D003637:DEAE-Dextran; D003911:Dextrans; D004285:Dogs; D004622:Embryo, Mammalian; D007668:Kidney / Q000648:ultrastructure; D007700:Kinetics; D051379:Mice; D008855:Microscopy, Electron, Scanning
D003911:Dextrans; D002482:Cellulose; D003636:DEAE-Cellulose; D003637:DEAE-Dextran