Antigen-specific human T cell factors. I. T cell helper factor: biololgic properties.
The in vitro induction and assay of an ovalbumin-specific human T cell helper factor are described. Peripheral blood T cells, cultured with ovalbumin in a Marbrook-Diener system, produce an antigen-specific factor(ThF120-OA), which can be purified by affinity chromatography. The in vitro studies with ThF120-OA pointed out that in the production of the factor as well as in the factor-B cell interaction the adherent cell determines the genetic restriction. The results of kinetic studies on T helper activities demonstrated that Thf120-OA provides an auxiliary activity at various moments during the differentiation of the human peripheral B cell into an antibody-secreting cell. The observed differences in the mode of action of Th cells and Th factor are discussed.
D001402:B-Lymphocytes / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology; D002448:Cell Adhesion; D000939:Epitopes; D005796:Genes; D006801:Humans; D007700:Kinetics; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D002352:Carrier Proteins; D000939:Epitopes; D010047:Ovalbumin