Cleavage of single strand oligonucleotides and bacteriophage phi X174 DNA by Msp I endonuclease.
Type II restriction endonucleases cleave duplex DNA at nucleotide sequences displaying 2-fold symmetry. Our data show that Msp I cleaves single strand oligonucleotides, d(G-A-A-C-C-G-G-A-G-A) and d(T-C-T-C-C-G-G-T-T) at 4 degrees, 25 degrees, and 37 degrees C reaction temperatures. The rate of cleavage of d(G-A-A-C-C-G-G-A-G-A) is several-fold faster than that of d(T-C-T-C-C-G-G-T-T). Single strand phi X174 DNA is also, cleaved by Msp I endonuclease giving well defined fragments. 5'-Nucleotide analysis of the fragments generated from single strand and replicating form DNA suggest that cleavage occurs at the recognition sequence d(C-C-G-G). The data show that Msp I endonuclease cleaves single strand oligonucleotides and prefers a recognition sequence surrounded by purine nucleotides. A general model for endonuclease cleavage of single strand and duplex DNA is presented.
D010584:Bacteriophage phi X 174 / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D019003:Deoxyribonuclease HpaII; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII