The determination of the percentages of G gamma and A gamma chains in human fetal hemoglobin by HPLC.
A newly developed HPLC procedure (1) was used to study the percentages of G gamma and A gamma chains in the Hb F from newborn and patients with sickle cell anemia. The method gives data comparable to those obtained with a more laborious chemical procedure. However, the presence of the A gamma T chain, i.e., the A gamma chain with an Ile replaced by Thr substitution at position 75, interferes with the determination of the G gamma to A gamma ratio because the A gamma T and G gamma chains have the same chromatographic mobility.
D000755:Anemia, Sickle Cell / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005312:Fetal Blood / Q000032:analysis; D005319:Fetal Hemoglobin / Q000032:analysis*; D006580:Genetic Carrier Screening; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D046911:Macromolecular Substances
D046911:Macromolecular Substances; D005319:Fetal Hemoglobin