The 2-5A (pppA2'p5'A2'p5'A) and protein kinase systems in interferon-treated and control cells.
With respect to interferon action in general: multisite models and analogies with hormone action are in vogue. More particularly, concerning the 2-5A system, the structure of 2-5A has been confirmed. We know that in the cell-free system and intact cell it is unstable and in the absence of a regenerating system transiently activates a nuclease. This nuclease shows some specificity; preferentially cleaving RNA on the 3'-side of UN doublets (UA and UU are preferred). When introduced into the intact cell 2-5A can inhibit protein and DNA synthesis and virus growth. In addition, both 2-5A and core occur naturally in amounts sufficient for them to play a part in the antiviral and antigrowth activities of interferon. Finally, it is possible that the 2-5A system may have a significance beyond its role in interferon action in the control of cell growth or development.
D000227:Adenine Nucleotides / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004789:Enzyme Activation; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012330:RNA, Double-Stranded; C023505:2',5'-oligoadenylate; D007372:Interferons; D011494:Protein Kinases