Radioimmunoassay for pancreatic glucagon based on specific derivatisation of the hormone.
A specific glucagon antiserum was generated in rabbits using a derivatisation of the methionine residue in glucagon. A sensitive and specific assay for glucagon has been developed. Interference with the assay system by plasma factors was abolished by both extraction of plasma samples and the production of glucagon-free plasma for each individual.
D000328:Adult; D000818:Animals; D007611:Aprotinin; D001120:Arginine; D004355:Drug Stability; D005215:Fasting; D005934:Glucagon / Q000031:analogs & derivatives / Q000097:blood* / Q000276:immunology; D005951:Glucose Tolerance Test; D006801:Humans; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
C018335:S-methylglucagon; D005934:Glucagon; D007611:Aprotinin; D001120:Arginine