Isolation of cells from rat seminal vesicles and epididymis and their use in studying androgen action.
Cells isolated enzymically from seminal vesicles and epididymides of normal and castrated rats were shown by electron microscopy to be intact and representative of the tissue. The cells synthesize and secrete tissue-specific proteins. Short-term incorporation of [3H]uridine and [35S]methionine was measured to determine the effects of castration on RNA and protein synthesis. Epididymal cells and tissue incorporated uridine at similar rates which were unaltered by castration. Similarly castration failed to diminish uridine incorporation by seminal vesicle cells and tissue. Therefore, androgens may principally control RNA degradation. A similar situation pertained to methionine incorporation by epididymal cells and tissue so here too control may be via protein degradation. In contrast, castration greatly decreased methionine incorporation by seminal vesicle tissue but not by isolated cells. Isolated cells were more active than in tissue, particularly those from castrated rats, and may be released from stromal-epithelial interactions and controls.
D000728:Androgens / Q000502:physiology*; D000818:Animals; D002369:Castration; D002469:Cell Separation; D004822:Epididymis / Q000166:cytology / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012669:Seminal Vesicles / Q000166:cytology / Q000502:physiology*; D013737:Testis / Q000502:physiology
D000728:Androgens; D012313:RNA