The use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of plasmodium berghei.
Intraerythrocytic parasites of Plasmodium vinckei and Plasmodium berghei were separated according to their developmental stages using discontinuous Percoll gradients. Contaminating nucleated blood cells such as leukocytes were removed by elutriation centrifugation. The stages were unequivocally identified in smears using a newly developed DNA-specific staining procedure with mithramycin and fluorescence microscopy. This stain can also be used to detect parasites in human blood of very low parasitemias. The combination of methods described has many possible applications in immunologic and biochemical parasite research.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D010962:Plasmodium berghei / Q000254:growth & development / Q000302:isolation & purification*; D008926:Plicamycin; D011205:Povidone; D012822:Silicon Dioxide; D013194:Staining and Labeling
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D008926:Plicamycin