Time: a new parameter for kinetic measurements in flow cytometry.
The measure of time was used as an additional parameter on an existing flow cytometer to study the kinetics of enzyme activities and cell-stain interactions. By correlating all fluorescent signals from single cells with time, the dynamics of a reaction can be followed for several minutes. This advanced application of flow cytometry is easily implemented and can be incorporated into any flow cytometer that has two-parameter analysis capability.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000201:enzymology; D003201:Computers; D006224:Cricetinae; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods; D013194:Staining and Labeling
D004247:DNA; D004950:Esterases