Species-dependent effects of dietary lindane and/or zineb on the activation of aflatoxin B1 into mutagenic derivatives.
Sprague-Dawley rats and Swiss mice were given diets containing lindane, 125 ppm, or zineb, 5200 ppm, or a mixture of both at the above-mentioned concentrations for 2 and 4 weeks. The effect of pesticide ingestion on the ability of liver S9 to metabolize aflatoxin B1 (AFB1) into mutagenic derivatives was tested by the Salmonella (TA100)/microsome test according to Ames. Control mouse-liver S9 was less efficient (13%) than the corresponding preparation from control rat liver. The ingestion of lindane produced a similar increase in the activities of both rat- (68%) and mouse-liver S9 (62%). Pretreatment with zineb inhibited (46%) rat-liver S9 but caused a marked increase (400%) in the activity of mouse-liver S9. Concomitant exposure to both pesticides showed that lindane released the inhibitory action of zineb on rat-liver S9 and reduced the stimulatory effect of zineb on mouse-liver S9. The inducing action of zineb in mice was a function of the dietary concentration of the pesticide. No effect was observed at dietary concentrations of zineb up to and including 500 ppm.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D004032:Diet; D004347:Drug Interactions; D001556:Hexachlorocyclohexane / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009152:Mutagenicity Tests; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects; D013045:Species Specificity; D013268:Stimulation, Chemical; D013859:Thiocarbamates / Q000494:pharmacology*; D015038:Zineb / Q000494:pharmacology*
D000348:Aflatoxins; D013859:Thiocarbamates; D001556:Hexachlorocyclohexane; D016604:Aflatoxin B1; D015038:Zineb