Immunocytochemistry: its applications and drawbacks for the study of gut neuroendocrinology.
Localization of gut peptide hormones by immunological staining methods is known generally as "immunocytochemistry". These immunostaining techniques depend upon the correct processing of the tissue under study and careful characterization of the antisera used. Many years of research in our laboratory have enabled us to present here our approach to the application and pitfalls of immunocytochemistry in the study of gut neuroendocrinology.
D000818:Animals; D000906:Antibodies; D005455:Fluorescent Antibody Technique; D005768:Gastrointestinal Hormones / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D010187:Pancreatic Hormones / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D012015:Reference Standards; D013048:Specimen Handling; D013194:Staining and Labeling / Q000379:methods
D000906:Antibodies; D005768:Gastrointestinal Hormones; D007106:Immune Sera; D010187:Pancreatic Hormones