Inhibition of lipid absorption by non-ionic hydrophobic surfactant Pluronic L-81, and its benzoyl esters.
The benzoyl ester of Pluronic L-81 (BEP) has been purified on an aluminium oxide column and fractionated on the basis of its mobility characteristics into three fractions, BEP I, II and III. Absorption studies were made with rats fed by gavage a lipid preparation containing [14C]triolein and [3H]cholesterol (control animals) and one of the fractions or Pluronic L-81, or crude BEP. After 4 h the amount of lipid absorbed and transported was calculated as the difference between the dose fed and the amount of lipid recovered from the gastric and intestinal luminal contents and intestinal mucosa. Pluronic L-81 was the most effective in inhibiting absorption of triolein but it did not inhibit absorption of cholesterol. BEP-II was almost as effective in inhibiting the absorption of triolein and also inhibited absorption of cholesterol. Crude BEP was less effective and BEP-I and III had only limited activity. Inhibition of triolein absorption by Pluronic L-81 may be partly related to its delaying action on gastric emptying. As purified BEP preparations had practically no effect on gastric emptying, their inhibiting activity involved intestinal mechanisms of lipid absorption.
D000042:Absorption; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism; D008297:Male; D011060:Poloxalene / Q000031:analogs & derivatives / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D013501:Surface-Active Agents / Q000494:pharmacology*; D014304:Triolein / Q000378:metabolism
D013501:Surface-Active Agents; D014304:Triolein; D011092:Polyethylene Glycols; C035554:pluronic dibenzoyl ester; D011060:Poloxalene; D002784:Cholesterol