Antiviral activities of hybrids of two major human leukocyte interferons.
Four hybrid human leukocyte interferon (LeIF or IFN-alpha) genes have been constructed by in vitro recombination of LeIF-A (IFN-alpha 2) and LeIF-D (IFN-alpha 1) genes at common restriction endonuclease sites located within their coding regions. These hybrid genes have been expressed in E. coli under trp promoter control. The interferons produced [LeIF-AD (BglII), -AD (PvuII), -DA (BglII), -DA (PvuII)] have antiviral properties distinct from the parental molecules LeIF-A and -D, varying considerably in their abilities to inhibit plaque formation by different viruses in a range of mammalian cells. All six of the cloned LeIFs exhibit the heat stability, pH 2 stability and antigenic specificity of natural leukocyte interferons.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004686:Encephalomyocarditis virus / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007372:Interferons / Q000235:genetics* / Q000494:pharmacology; D007962:Leukocytes / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009876:Operon; D010957:Plasmids; D018139:Simplexvirus / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D004274:DNA, Recombinant; D007372:Interferons; D004262:DNA Restriction Enzymes