Preparation of polysaccharide derivatives containing amino groups for application in affinity chromatography.
We describe here simple methods to introduce spacer arms with amino functional group into polysaccharides. (Formula: see text) The technique includes the following steps: Compounds comprising epoxide group(s) are introduced into polymer supports to form the spacers. Then, reactive groups on the other side of the introduced spacers are reacted with ammonia. Since spacers are attached via ether linkage to the supports, the escape of ligands from specific sorbents can be prevented. Moreover, no charged groups arise on the carrier during the activation procedure, thus decreasing the nonspecific protein retention.
D000641:Ammonia; D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D003911:Dextrans; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D011134:Polysaccharides; D012685:Sepharose
D003911:Dextrans; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D011134:Polysaccharides; D000641:Ammonia; D002482:Cellulose; D012685:Sepharose