A simple immunofluorescence assay for C1q binding.
Antibody-independent binding of B1q to Crithidia luciliae kinetoplast DNA was demonstrated by immunofluorescence microscopy. The method allows detection of C1q in human sera diluted 1/20--1/40 and in 5--10 micrograms/ml concentrations of isolated C1q. Various substances known to interfere with the early events of complement activation inhibited binding of C1q to kinetoplasts. The method may be used as a functional assay for serum C1q.
D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003172:Complement C1 / Q000276:immunology*; D003171:Complement Pathway, Classical; D003421:Crithidia / Q000276:immunology; D004247:DNA / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D011951:Receptors, Complement / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D003172:Complement C1; D011951:Receptors, Complement; D005719:gamma-Globulins; D004247:DNA