Immunohistochemical localization of several cytoskeletal proteins in inner ear sensory and supporting cells.
Several structural and contractile proteins have been searched for with immunohistochemical methods using antibodies directed against these proteins. Three types of preparations from the guinea pig have been used: isolated stereocilia from the utricle, organ of Corti fragments obtained by cellular dissociation and 0.2-1 micrometer sections obtained by cryoultramicrotomy. The main finding is that different sets of proteins compose the cytoskeleton in supporting cells and the mechanoreceptor structures of the sensory cells. Thus, actin was found in association with fimbrin in the mechanoreceptive region of hair cells, whereas supporting cells, although rich in actin, did not reveal fimbrin. Instead tubulin was seen together with actin in supporting cells which also exhibited prekeratin. Fimbrin appears to function as a protein capable of making bundles and networks from actin filaments. Its exclusive presence in the mechanosensitive region of the sensory cells is possibly related to the function of these cells as mechanoreceptors.
D000199:Actins / Q000032:analysis; D000818:Animals; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000032:analysis*; D007633:Keratins / Q000032:analysis; D007760:Labyrinth Supporting Cells / Q000032:analysis*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000032:analysis; D008840:Microfilament Proteins; D009925:Organ of Corti / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012444:Saccule and Utricle / Q000032:analysis; D014404:Tubulin / Q000032:analysis
D000199:Actins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D011498:Protein Precursors; D011506:Proteins; D014404:Tubulin; C026742:plastin; C004818:prekeratin; D007633:Keratins