title: Comparative studies of a monoclonal IgG lambda and two IgE kappa components from an individual patient: evidence for shared idiotypic determinants between the two IgE proteins but not with the IgG lambda protein.
abstract: An IgG lambda and two IgE kappa proteins were isolated from the serum of a patient (DB) with anaemia associated with myelofibrosis. Idiotype-specific antisera produced against the three proteins were studied by precipitation in double immunodiffusion and by enzyme-linked immunosorbent assay (ELISA) and indicated that the two IgE kappa components shared idiotypic determinants while the IgG lambda protein did not show any cross-idiotypic reaction. The idiotypic determinants of the three proteins were not present in pooled normal serum, normal IgG, normal IgE or IgE from a patient with multiple myeloma.
mesh_terms: D000740:Anemia / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000276:immunology*; D007130:Immunoglobulin Idiotypes; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D008875:Middle Aged; D055728:Primary Myelofibrosis / Q000276:immunology
chemical_list: D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007073:Immunoglobulin E