A technique of silver impregnation for nervous tissues with some thiocyanates.
Taking advantage of some properties of thiocyanates, a technique of silver impregnation for nerve fibres in the human central nervous system is described. The following procedure is suggested: frozen sections 25-35 micrometer thick, 5-10% aqueous solution of ammonium thiocyanate or potassium thiocyanate, 10% aqueous solution of silver nitrate, ammoniacal silver solution, reduction in 20% formalin, gold toning, dehydrating and mounting. By this procedure, fine fibres appear in the color range from purple to dark purple on the almost colorless background.
D002467:Cell Nucleus / Q000032:analysis; D002525:Cerebellar Cortex / Q000166:cytology; D003593:Cytoplasm / Q000032:analysis; D006801:Humans; D009412:Nerve Fibers / Q000032:analysis*; D009474:Neurons / Q000032:analysis* / Q000648:ultrastructure; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods; D013861:Thiocyanates
D013861:Thiocyanates; D012835:Silver Nitrate