Antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (ELISA) for detecting infectious bronchitis viral antibodies in chicken serum.
The conditions of a rapid, indirect-enzyme linked immunosorbent assay for Infectious Bronchitis Virus (IBV) antibodies have been established. Optimal sensitivity was obtained using 10 micrograms/ml protein concentration of the Mass 41 strain purified from infected allantoic fluid. Specificity was demonstrated with Newcastle Disease Virus (NDV) antigen-antibody system. Negligible crossreactions were observed. After bromelain or lipase treatment IBV had an ELISA reactivity similar to untreated particles suggesting that peripheral constituents of IBV play a minor role when whole virus is absorbed on solid phase. The method offers a simple and specific antibody assay which could be used for the laboratory diagnosis of avian infectious bronchitis.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D002645:Chickens / Q000276:immunology*; D003332:Coronaviridae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes; D007124:Immunoenzyme Techniques / Q000379:methods*; D001351:Infectious bronchitis virus / Q000276:immunology*
D000914:Antibodies, Viral; D000939:Epitopes