title: Chemical evidence for the separation of G gamma and A gamma chains by polyacrylamide gel electrophoresis in urea and triton X-100.
abstract: Polyacrylamide gel electrophoresis in urea and Triton X-100 of a hemolysate from human fetal red blood cells produces four major protein bands: alpha, beta, and 2 gamma globin chains. We have verified that the latter two are the G gamma and A gamma globin chains which have respectively glycine or alanine at position 136. After incorporation of either [3H] alanine or [3H] glycine into newly synthesized globin each gamma chain was isolated by preparative electrophoresis. The chains were cleaved with cyanogen bromide at methionines 55 and 133, then subjected to automated sequencing, and the residues from each sequencer turn counted. Glycine incorporation was detected for the third turn (position 136) of the G gamma chain and alanine for the A gamma. Substantial metabolic conversion of [3H] glycine to serine and proline was also noted.
mesh_terms: D000409:Alanine; D000596:Amino Acids / Q000032:analysis; D001322:Autoanalysis; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005319:Fetal Hemoglobin / Q000032:analysis / Q000302:isolation & purification*; D005914:Globins / Q000302:isolation & purification*; D005998:Glycine; D006801:Humans; D017830:Octoxynol; D010446:Peptide Fragments / Q000302:isolation & purification; D011092:Polyethylene Glycols; D014508:Urea
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D011092:Polyethylene Glycols; D014508:Urea; D017830:Octoxynol; D005914:Globins; D005319:Fetal Hemoglobin; D000409:Alanine; D003488:Cyanogen Bromide; D005998:Glycine