Immunization of sheep with larval antigens of Lucilia cuprina.
Sheep were immunized with antigens extracted from third-instar larvae of L. cuprina. This procedure produced substantial titres of circulating antibody as measured by solid-phase radioimmunoassay or immunodiffusion or by both techniques. However, immunization did not confer protection against subsequent implant challenge with first-instar larvae. In vitro studies indicated that pooled sera from immunized sheep (mean immunodiffusion titre = 3) significantly reduced larval survival. Antigen specificity and the modulating effects of concomitant humoral responses to larval challenge are discussed in relation to the findings.
D000818:Animals; D004175:Diptera / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D007114:Immunization / Q000662:veterinary*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007814:Larva / Q000276:immunology; D009198:Myiasis / Q000517:prevention & control*; D012756:Sheep / Q000276:immunology*
D000939:Epitopes