A simple method to obtain an in vitro primary immune response to dinitrophenyl coupled to bovine gamma-globulin.
This paper describes a simple method of achieving a primary in vitro response to dinitrophenyl coupled to a soluble protein. Mice are carrier-immunized, then irradiated and restored with spleen cells from normal donors. Spleen cells are then cultured in the presence of dinitrophenyl on homologous carrier. The anti-hapten antibody synthesis is dose-dependent, and the highest response is obtained with the lowest dose used in the experiments. The higher level of hapten conjugation reduces the immune response.
D000818:Animals; D000917:Antibody Formation; D002352:Carrier Proteins / Q000276:immunology; D002417:Cattle; D004136:Dinitrobenzenes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D051379:Mice; D008811:Mice, Inbred DBA; D009163:Mycobacterium bovis / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology*
D002352:Carrier Proteins; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D005719:gamma-Globulins