[Determination of the relative RNA-content of liver cell nuclei with gallocyanin-chromealum-staining (author's transl)].
The relative RNA-content of liver cell nuclei from histological slides of rat liver was determined by automatical microscope picture analysis. The slides were stained with Gallocyanin-Chromealum. Extinction measurements are made before and after perchloracid extraction of RNA for the same slide. The changes of extinction measurements caused by the changes of slide thickness and of tissue inhomogeneity could be lowered using this method.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002857:Chromium; D017608:Chromium Compounds; D004396:Coloring Agents; D008099:Liver / Q000648:ultrastructure*; D010078:Oxazines; D017680:Potassium Compounds; D012313:RNA / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods*; D013431:Sulfates
D017608:Chromium Compounds; D004396:Coloring Agents; D010078:Oxazines; D017680:Potassium Compounds; D013431:Sulfates; D002857:Chromium; C020720:chrome alum; D012313:RNA; C007062:gallocyanine
Bestimmung des relativen RNA-Gehaltes von Leberzellkernen bei der Färbung mit Gallocyanin-Chromalaun.