Immunochemical evidence for exocytosis in isolated chromaffin cells after stimulation with depolarizing agents.
After chemical stimulation with depolarizing agents (Ba2+ or Ca2+/carbachol) isolated living chromaffin cells display a drastically increased binding capacity for anti-DBH, distributed spotwise on or near the outer cell membrane. This effect is inhibited by noradrenaline; it is not evoked by the non-exocytotically releasing agents tyramine and reserpine. the effect of apparent externalization of DBH is paralleled by the observation of a DBH-dependent binding of 125I-labelled protein A upon the same depolarizing stimuli. These observations are discussed as possible evidence for exocytotic activities.
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002837:Chromaffin Granules / Q000032:analysis / Q000276:immunology*; D002838:Chromaffin System / Q000276:immunology*; D004299:Dopamine beta-Hydroxylase / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000378:metabolism; D005719:gamma-Globulins / Q000032:analysis
D000906:Antibodies; D009467:Neuromuscular Depolarizing Agents; D013205:Staphylococcal Protein A; D005719:gamma-Globulins; D004299:Dopamine beta-Hydroxylase