Continuous automated assay of alpha-amylase release from superfused rat salivary gland.
A method of continuous automated amylase assay is described. This relies on the absorption of iodine by starch to produce a blue color that can be quantified colorimetrically. Digestion of the starch by amylase released from parotid tissue slices reduces the intensity of the color formed, allowing quantification of the amylase released. The assay in sensitive to 0.05 U/amylase/ml and linear up to 13 U/ml. Typical tissue responses to acetyl-beta-methylcholine and isoprenaline are presented.
D000681:Amylases / Q000032:analysis*; D000818:Animals; D001322:Autoanalysis; D003124:Colorimetry / Q000379:methods; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D012469:Salivary Glands / Q000187:drug effects / Q000201:enzymology*; D000516:alpha-Amylases / Q000032:analysis*
D008688:Methacholine Compounds; D016210:Methacholine Chloride; D000681:Amylases; D000516:alpha-Amylases; D007545:Isoproterenol