The biosynthesis of oligosaccharide-lipids. Isolation of an oligosaccharide-P-P-lipid acceptor.
An oligosaccharide-P-P-lipid has been isolated from porcine liver by extraction with organic solvents and purified by chromatography on silica gel and DEAE-cellulose. The purified oligosaccharide-lipid was shown to contain mannose and N-acetylglucosamine in an approximate ratio of 1:1 and our results suggest that the major oligosaccharide component in the preparation was a tetrasaccharide with the composition (Man)2 (GlcNAc)2. When the oligosaccharide-lipid was incubated with GDP-[14C]mannose and a solubilized enzyme preparation from rabbit liver in the presence of MgCl2, three radioactive products could be isolated. The oligosaccharides in the products could be identified as a penta-, a hexa-, and a heptasaccharide. These products were formed by the stepwise addition of mannose to the growing oligosaccharide chain and GDP-mannose was indicated as the glycosyl donor in each reaction.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D006017:Glycolipids / Q000096:biosynthesis* / Q000302:isolation & purification; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000032:analysis; D011817:Rabbits; D013552:Swine
D002241:Carbohydrates; D006017:Glycolipids; D009844:Oligosaccharides; D009943:Organophosphorus Compounds; D006155:Guanosine Diphosphate Mannose