Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells.
The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002024:Bufo marinus; D008970:Molecular Weight; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000235:genetics*; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
D011506:Proteins; D000450:Aldosterone; D012313:RNA