A rapid method for the detection of antibodies to cell surface antigens: a solid phase radioimmunoassay using cell membranes.
Cell membranes isolated from murine lymphocytes or ascites tumors bind tightly to the surface of flexible plastic microtiter plates in the absence of additional proteins. This allows the detection of membrane associated molecules by specific antibodies and thus forms the basis for a rapid and sensitive radioimmunoassay for antibodies to membrane-bound components. The assay compares favorably with a variety of methods currently used to detect antibodies to cell surface antigens. The assay detects a variety of well characterized murine cell surface antigens (H-2, I-A, T-200, Thy-1.2, Ig). The level of antibody binding to membranes on plates correlates well with antigen density on intact cells. A modification of the assay involving competition between cross-reacting antibodies allows detection and resolution of closely spaced antigenic determinants.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011863:Radioimmunoassay / Q000379:methods*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000906:Antibodies; D000954:Antigens, Surface; D000939:Epitopes