title: The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.
abstract: Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012333:RNA, Messenger; D007372:Interferons; D012194:RNA-Directed DNA Polymerase