Nature of albumin exported from the frog oocyte following microinjection of mouse liver mRNA.
Microinjections of mouse liver mRNA into Xenopus laevis oocytes induced efficient export of a polypeptide with an apparent Mr or 68,000 which was immunoprecipitable with anti-mouse albumin antibody. Analysis of the anti-albumin precipitate of the exported protein by two-dimensional gel electrophoresis showed that the electrophoretic behavior precisely coincided with that of authentic mouse serum albumin. This result indicates that the Xenopus oocyte may perform secretory processes similar or identical to those occurring in liver cells with respect to the processing of albumin.
D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D014981:Xenopus
D000418:Albumins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA