Culture conditions modify the effects exerted by human fetal alpha-fetoprotein (AFP) on some lymphocyte functions in vitro.
Incorporation of (methyl-3H)-thymidine to DNA of allogeneically and phytohaemagglutinin (PHA)-stimulated lymphocytes cultured in the presence of various concentrations of AFP from human blood cord serum was investigated. The cells were kept in Parker medium supplemented with autologous plasma, calf serum or human AB serum. AFP in concentrations of 5-75 micrograms per one millilitre of culture caused various degrees of inhibiton of responding lymphocyte proliferation. These effects were dependent on the composition of the culture medium, type of lymphocyte stimulation and their individual properties.
D000328:Adult; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006648:Histocompatibility; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology*
D003470:Culture Media; D010835:Phytohemagglutinins; D000509:alpha-Fetoproteins; D004247:DNA