title: Effect of changes in circulating amylase levels on amylase output in bile.
abstract: The relation between plasma and biliary amylase activity and their relationship to the functional state of the pancreas were studied in anesthetized rabbits. Repetitive intravenous injections of cholecystokinin resulted in a 25-fold rise in the secretion of amylase via the pancreatic duct, followed at first by a 50% increase in plasma amylase concentration and later by a 270% increase in biliary amylase concentration. There was then a gradual, roughly synchronous decline in both plasma and biliary values toward basal level despite a continued highly augmented rate of pancreatic ductal secretion. "Near-total" pancreatectomy completely abolished the effect. These observations are consistent with a cholecystokinin-induced basolateral secretion of amylase from pancreas into blood and its subsequent movement from blood into bile down a concentration gradient. The output of amylase in bile, however, was quite small and does not suggest that biliary transport of amylase has an important function either as a means of secreting and recycling digestive enzyme into the gut or as a major excretory pathway for circulating amylase in the rabbit.
mesh_terms: D000681:Amylases / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000187:drug effects / Q000201:enzymology*; D002766:Cholecystokinin / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology; D011817:Rabbits
chemical_list: D002766:Cholecystokinin; D000681:Amylases