A polymorphic antigen displaying different molecular weights in sheep and cattle sera.
The polymorphic antigen B1 is present, exhibiting different molecular weights, in both sheep and cattle sera. The molecular weight of the antigen, purified from both sera by a specific immunoabsorbent, was analysed by SDS polyacrylamide gel electrophoresis and calibrated gel filtration. Results indicate that only one subunit, 35 000 MW, forms cattle B1 while a further subunit, 60 000 MW, is present in sheep B1. The antigen activity is localized on the 35 000 MW subunit, which appears to be the same in both species.
D000818:Animals; D000941:Antigens / Q000235:genetics*; D002417:Cattle / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000235:genetics; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D012756:Sheep / Q000097:blood*
D000941:Antigens; D000939:Epitopes