title: A murine leukemia virus yield-reduction semi-microassay for interferon.
abstract: Interferon (IF) treatment of murine cells chronically producing infectious leukemia virus results in a decreased yield of extracellular virus. We have utilized this principle to develop a useful semi-microassay for IF. The assay incorporates the desirable features of both virus yield-reduction and plaque-reduction assays, yet has few of the undesirable features associated with many IF assays. Being a semi-micro assay, it is amenable to the screening of multiple samples for IF and is economical with respect to cell culture materials, reagents and amount of sample required for testing.
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D007372:Interferons / Q000032:analysis* / Q000494:pharmacology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000254:growth & development; D010948:Viral Plaque Assay
chemical_list: D007372:Interferons