title: Ganglion cell distribution in the retina of the mouse.
abstract: The distribution of ganglion cells in the mouse retina was studied with the use of Nissl criteria for distinguishing cell types in the ganglion cell layer. Retrograde filling with horseradish peroxidase (HRP) from the optic fiber tract helped to validate Nissl criteria and served to identify displaced ganglion cells. We estimated a total of 117,000 nonvascular cells in the ganglion cell layer; of these, 70,000 were probably ganglion cells, and 47,000 could not be classified. The density of the presumed ganglion cells was highest-more than 8000 cells/mm2-just temporal to the optic disk, and lowest-less than 2000 cells/mm2-in the most dorsal retina. The retinal region with highest ganglion cell density was slightly elongated in a nasotemporal direction. About 2% of all HRP-filled ganglion cells had their cell bodies in the inner nuclear layer. These displaced cells differed in topographical distribution from the normally positioned ganglion cells: although occurring throughout the retina, they were more common along the retinal periphery. Measurements of ganglion cell areas showed a tendency toward larger size with eccentricity. At no retinal location did cell-size histograms reveal clearly separate size classes.
mesh_terms: D000818:Animals; D001369:Axons; D002452:Cell Count; D006735:Horseradish Peroxidase; D051379:Mice / Q000033:anatomy & histology*; D009474:Neurons / Q000032:analysis*; D009562:Nissl Bodies; D012160:Retina / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D006735:Horseradish Peroxidase