The Feulgen reaction: importance of the wash after hydrolysis.
Washing in absolute ethanol after FEULGEN hydrolysis minimizes losses of DNA. The hydrolysis in HCl diluted in tetrahydrofuran is not superior in this respect to conventional hydrolysis if this is followed by an ethanol wash. These conclusions are based on cytophotometric and radiometric experiments made on salivary glands of sciarid larvae.
D000818:Animals; D004247:DNA / Q000032:analysis*; D004175:Diptera / Q000032:analysis; D000431:Ethanol; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D006868:Hydrolysis; D007814:Larva / Q000032:analysis; D012469:Salivary Glands / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods
D000431:Ethanol; D004247:DNA