title: Sequence of the CNBr peptide containing the putative essential tyrosine of Bacillus amyloliquefaciens alpha amylase.
abstract: The amino acid sequence of the cyanogen bromide peptide (peptide B) containing the putative essential tyrosine residue in Bacillus amyloliquefaciens alpha-amylase (EC 3.2.1.1.) was determined. It is composed of 73 amino acids and the "active" tyrosine residue is the N-terminus of the peptide. Upon iodination of the whole enzyme by means of a lactoperoxidase-catalyzed reaction, a minimum of eight tyrosine residues are iodinated. Four of these belong to peptide B. Among the cyanogen bromide peptides, B is the most readily iodinated one. Hence, it is predicted that peptide B is an exposed segment of the amylase molecule.
mesh_terms: D000595:Amino Acid Sequence; D000681:Amylases / Q000032:analysis*; D001407:Bacillus / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide; D010446:Peptide Fragments / Q000032:analysis; D014443:Tyrosine / Q000032:analysis*; D000516:alpha-Amylases / Q000032:analysis*
chemical_list: D010446:Peptide Fragments; D014443:Tyrosine; D000681:Amylases; D000516:alpha-Amylases; D003488:Cyanogen Bromide