Interaction of substance P with dispersed pancreatic acinar cells from the guinea pig. Stimulation of calcium outflux, accumulation of cyclic GMP and amylase release.
In dispersed acinar cells from the guinea pig pancreas, substance P (SP) was found to stimulate outflux of 45Ca, cellular accumulation of cyclic GMP, and release of amylase. Maximal effects on accumulation of cyclic GMP and release of amylase were obtained with 3 x 10(-8) M of SP, 10(-7) M of Sp caused maximal outflux of 45Ca. These effects corresponded to 30-50% of the maximal effects obtained with caerulein, a cholecystokinin-like decapeptide. The concentrations of SP required for stimulation of 45Ca outflux, accumulation of cyclic GMP, and release of amylase correspond well with those which affect binding of 125I-tyr8-SP to pancreatic acinar cells.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002108:Ceruletide / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism*; D006168:Guinea Pigs; D010179:Pancreas / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D013268:Stimulation, Chemical; D013373:Substance P
D013373:Substance P; D002108:Ceruletide; D000681:Amylases; D006152:Cyclic GMP; D002118:Calcium