Transformation induced by Abelson murine leukemia virus involves production of a polypeptide growth factor.
Rat embryo fibroblasts transformed by Abelson murine leukemia virus (MuLV) produce and release a transforming growth factor (TGF). Production of this factor is correlated with a tyrosine-specific protein kinase that is functionally active and is associated with the major Abelson MuLV gene product, P120. Transformation-defective mutants of Abelson MuLV do not transform cells, do not have their virus coded transforming gene product phosphorylated in tyrosine, and do not induce TGF production. Abelson MuLV-induced TGF morphologically transforms cells in culture, competes with 125I-labeled epidermal growth factor (EGF) for binding to cell receptors, and induces phosphorylation of tyrosine acceptor sites in the 160,000-dalton EGF membrane receptor. After purification to homogeneity, Abelson virus-induced TGF migrates as a single polypeptide with an apparent size of 7400 daltons as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
D000011:Abelson murine leukemia virus; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D066246:ErbB Receptors; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D019000:Phosphotyrosine; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D015291:Transforming Growth Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D010455:Peptides; D011956:Receptors, Cell Surface; D019000:Phosphotyrosine; D014443:Tyrosine; D015291:Transforming Growth Factors; D066246:ErbB Receptors