[Incorporation of [2-14C orotic] acid into chromatin RNA of tissues of animals of various ages].
The structure and biological activity (the level of the labelled precursor incorporation into RNA) of active and repressed chromatin of the liver and small intestine mucosa were studied in adult (6-8 months) and old (24-26 months) rats. The content of repressed chromatin fraction in both tissues is found to increase with age. In the liver of old rats the level of [14C[ orotic acid incorporation into RNA of chromatin fractions decreases, radioactivity of the acid-soluble fraction being unchanged. In the small intestine mucosa a high leve of [14C] orotic acid incorporation into chromatin RNA with ageing is due to an increase in permeability of the mucosa cells.
D000375:Aging; D000818:Animals; D002250:Carbon Radioisotopes; D002843:Chromatin / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000254:growth & development* / Q000378:metabolism; D008099:Liver / Q000254:growth & development* / Q000378:metabolism; D009963:Orotic Acid / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
D002250:Carbon Radioisotopes; D002843:Chromatin; D009963:Orotic Acid; D012313:RNA
Vkliuchenie [2-14C] orotovoi kisloty v RNK khormatina tkanei zhivotnykh raznogo vozrasta.