[Incorporation of H3 uridine in chick embryo skin cultured in media containing histones].
In this paper the authors have carried out further research on the differentiation of six-day limb skin from chick embryos cultured in vitro in mediam containing histones. When 3H-uridine is added to this modified medium, a decrease of 3H-uridine uptake and incorporation is observed. The authors conclude that in their system the histones not only interfere with the regulation of genetic transcription by inhibiting DNA-dependent RNA synthesis, but they also change cellular permeability.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002642:Chick Embryo; D003470:Culture Media; D006657:Histones / Q000494:pharmacology*; D009924:Organ Culture Techniques; D012313:RNA / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014529:Uridine / Q000378:metabolism*
D003470:Culture Media; D006657:Histones; D012313:RNA; D014529:Uridine
Incorporazione di urinina H3 in espianti di cute di embrione di pollo coltivati con istoni.