Bovine hypothalamic poly(A)-rich RNA-directed synthesis of a common precursor to the nonapeptide arginine vasopressin and neurophysin II. Immunological identification and tryptic peptide mapping.
The common precursor to arginine vasopressin (AVP) and neurophysin II (NpII) has been synthesized in a reticulocyte lysate system directed by bovine hypothalamic poly(A)-rich RNA. The precursor, identified with antibodies raised against neurophysin II and arginine vasopressin, has an apparent Mr of 21 000 (21 k). The specificity of the immune reaction has been shown by competition experiments using excess amounts of a variety of unlabeled peptides. With anti Np II, but not with anti AVP, a second neurophysin precursor with an apparent Mr of 18 000 (18 k) has been identified. Comparison of the tryptic maps obtained from the 21 k and 18 k precursors shows that both products give rise to the four [35S]cysteine-labeled neurophysin II-peptide fragments; however, only the 21 k yields an AVP-like tryptic peptide, identified with antibodies raised against AVP. The possible implications of the two precursors, one consisting of AVP and Np II, the other of Np II only, are discussed.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001127:Arginine Vasopressin / Q000235:genetics*; D002417:Cattle; D007031:Hypothalamus / Q000378:metabolism*; D009481:Neurophysins / Q000235:genetics*; D010446:Peptide Fragments / Q000032:analysis; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014357:Trypsin
D000906:Antibodies; D000936:Antigen-Antibody Complex; D009481:Neurophysins; D010446:Peptide Fragments; D011498:Protein Precursors; D012333:RNA, Messenger; D001127:Arginine Vasopressin; D011061:Poly A; D012313:RNA; D014357:Trypsin