Timing and localization of the stimulatory effect of follicle-stimulating hormone on uridine incorporation in the mouse testis in vivo.
Human pituitary FSH increased the incorporation of [5-(3)H]uridine into RNA in vivo in the testes of intact 9-day-old mice and of hypophysectomized adults. In both groups the effect was greatest 8 h after administration of 5--7.5 i.u. FSH. Autoradiographs were prepared from the testes of hypophysectomized adult mice given subcutaneous injections of FSH or of 0.9% saline 6 h, and [5-(3)H]uridine 1.5 h, before death. Treatment with FSH caused statistically significant increases in the density of silver grains over the nuclei of Sertoli cells, type A and intermediate spermatogonia, and preleptotene and mid-pachytene primary spermatocytes. It was concluded that FSH has a generalized stimulatory action on RNA synthesis in the nuclei of Sertoli cells and those types of germinal cell which synthesize RNA most actively.
D000818:Animals; D001345:Autoradiography; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007016:Hypophysectomy; D008297:Male; D051379:Mice; D012313:RNA / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014529:Uridine / Q000378:metabolism*
D012313:RNA; D005640:Follicle Stimulating Hormone; D014529:Uridine