Establishment of a pluripotent embryonal carcinoma cell line not expressing SSEA-1 and ECMA-7 phenotypes.
A murine embryonal carcinoma (EC) cell line heterozygous for t0 recessive lethal mutation has been established from an embryo-derived transplantable teratocarcinoma TC1Ph of the genotype (129-T/t0 X C3H/Di)t0/+. The EC cell line, designated EC1Ph, and two cloned sublines, EC1Ph/a and EC1Ph/b, maintain the diploid karyotype (40, XY) and give rise to teratocarcinomas with differentiated derivatives of EC cells after inoculation into syngeneic recipients. The cloned sublines express low or zero amounts of SSEA-1 and ECMA-7 stage-specific antigens. At some passages, the EC1Ph line and the cloned subline EC1Ph/b express a significant quantity of class I H-2 antigens. This unusual EC phenotype resembles that of human teratocarcinoma cell lines.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics*; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D003584:Cytological Techniques; D006017:Glycolipids / Q000032:analysis / Q000235:genetics*; D016256:Lewis X Antigen; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D009368:Neoplasm Transplantation; D010641:Phenotype; D011863:Radioimmunoassay; D013724:Teratoma / Q000235:genetics* / Q000473:pathology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006017:Glycolipids; D016256:Lewis X Antigen