title: Phosphorylation in vitro and in vivo of the wheat embryo RNA polymerase II.
abstract: One of the large subunits (220 000 daltons) of the wheat embryo RNA polymerase II was demonstrated to undergo phosphorylation with [gamma-32P]ATP in a reaction catalysed by a homologous protein kinase preparation. The same subunit was also observed to be phosphorylated in vivo, at the onset of germination. The phosphorylation resulted in a moderate increase of the RNA polymerase activity.
mesh_terms: D001345:Autoradiography; D046508:Culture Techniques; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification; D012313:RNA; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology*
chemical_list: D010761:Phosphorus Radioisotopes; D012313:RNA; D011494:Protein Kinases; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases