Monoclonal antibodies to a human prostate antigen.
Three monoclonal antibodies reactive with a purified extractable Mr 34,000 prostate antigen (PA) have been prepared by fusing splenocytes of BALB/c mice preimmunized with purified PA with the NS1 mouse myeloma cell line. The three antibodies were all of the IgG-1 subclass. The antibodies defined two noncross-blocking unique determinants on PA; each present as one site per molecule. IF3 defined one antigenic site and 2G7 and 1C5 defined another antigenic determinant. All of the antibodies reacted with PA in a solid-phase radioimmunoassay and immunoprecipitated 125I-labeled PA. Absorption and sandwich radioimmunoassays showed PA in prostate tissues but not in tonsil, liver, or kidney. Immunoperoxidase staining of formalin-fixed paraffin-embedded sections of benign prostatic hyperplasia and prostatic carcinoma revealed strong prostate epithelial reactivity. None of the antibodies showed reactivity with prostate membrane preparations. A sandwich radioimmunoassay used 2G7 as a plate coat. 125I-labeled 1F3 was used to detect 5 ng PA per ml in sera of patients with prostate cancer. These results confirm previous observations regarding the specificity of PA and shed new evidence for its intracellular localization.
D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D011467:Prostate / Q000276:immunology* / Q000648:ultrastructure; D011470:Prostatic Hyperplasia / Q000276:immunology; D011471:Prostatic Neoplasms / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D007074:Immunoglobulin G