title: The hexa- and pentapeptide extension of proalbumin. I. Chemical synthesis of serum albumin propeptides.
abstract: The proalbumin hexapeptide extension was synthesized beginning from the C-terminal end by stepwise N-terminal peptide chain elongation starting from N-tert-butyloxycarbonyl-(Ng-nitro)arginyl-(Ng-nitro)arginine 4-nitrobenzyl ester; [alpha](20)365-12 degrees C (c = 1; dimethylformamide). The other amino acids were incorporated by excess mixed anhydrides of Ddz-amino acids (Ddz; 3,5-dimethoxyphenylisopropyloxycarbonyl) yielding the fully protected hexapeptide in crystalline quality. After removal of the protective groups by acid treatment and hydrogenation the peptide was purified by Dowex ion-exchange and Sephadex chromatography. The purity was confirmed by thin-layer chromatography and amino acid analysis.
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D000939:Epitopes; D009842:Oligopeptides / Q000138:chemical synthesis*; D009904:Optical Rotation; D011228:Prealbumin; D012709:Serum Albumin
chemical_list: D000939:Epitopes; D009842:Oligopeptides; D011228:Prealbumin; D012709:Serum Albumin