Relationship of idiotypes of the anti-p-azophenylarsonate antibodies of A/J and BALB/c mice.
It has previously been shown that A/J anti-Ar antibodies contain 2 different families of cross-reactive idiotypes, referred to as the major and minor idiotypes populations. The present report shows that the minor A/J idiotype is related to a major idiotype of BALB/c anti-Ar antibodies. Anti-idiotype directed against the minor A/J idiotype binds 5 to 10% of A/J anti-Ar but an average of about 40% of BALB/c anti-Ar. This BALB/c population corresponds to the major BALB/c anti-Ar idiotype. For individual BALB/c anti-Ar preparations the maximum percentages of antibody bound by anti-id directed to A/J or BALB/c anti-Ar are very similar. Anti-id reactive with the minor A/J idiotypic population suppressed the formation of the BALB/c major idiotype when injected into BALB/c mice. Adsorption experiments showed that only about one-third of the minor A/J population is related to the BALB/c idiotype and that the expression of this idiotype is highly variable in individual A/J sera. Several types of evidence, obtained with hybridoma products expressing the major A/J idiotype, revealed no detectable relationship between the major A/J and BALB/c anti-Ar idiotypes.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001391:Azo Compounds / Q000276:immunology*; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D000939:Epitopes; D006822:Hybrid Cells / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007165:Immunosuppression Therapy; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013045:Species Specificity; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000906:Antibodies; D001391:Azo Compounds; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010132:p-Azobenzenearsonate