Functional similarities of AeE alpha Ia molecules as determined by analysis with T-cell clones.
Recognition of AeE alpha Ia antigens at the functional level was investigated using T-cell clones. The reactivities of an alloreactive and an antigen-reactive clone, both of which recognized AeE alpha Ia molecules, were compared on a panel of stimulator/antigen-presenting cells of various genotypes. The two clones recognized all tested AebE alpha x Ia molecules, where x is a haplotype capable of expressing an Ia.7-bearing E alpha polypeptide. Ia antigen recognition by either clone could be inhibited by the monoclonal antibody Y-17, which recognizes a combinatorial serologic determinant on certain AeE alpha molecules. There were no differences in the recognition of Ia by the alloreactive versus the antigen-reactive clone, suggesting that Ia antigens are recognized by the two clones in a fundamentally similar way. The recognition of these various Ia molecules by the two cloned T-cell lines provides evidence that the E alpha polypeptides from H-2 haplotypes k, d, r, and u are functionally indistinguishable.
D000818:Animals; D000911:Antibodies, Monoclonal; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000949:Histocompatibility Antigens Class II