Immunological studies on ovine fetuin: radioimmunoassay and comparison with related antigens.
A radioimmunoassay for fetuin is described. It uses [125I]fetuin and rabbit antisera. The bound antigens are precipitated by anti-rabbit globulins coupled to Sepharose. This assay has been used to compare the immunological properties of ovine and bovine fetuin. It allows to detect a serum antigen cross-reacting with fetuin in pig species. A very faint reaction is observed with human fetal serum, that does not support the existence of a fetuin like antigen in human.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001667:Binding, Competitive; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D011247:Pregnancy; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D000509:alpha-Fetoproteins / Q000276:immunology*
D000941:Antigens; D007106:Immune Sera; D000509:alpha-Fetoproteins