Determinant specific suppression of antigen-induced T cell proliferation in the guinea pig. II. Determinant specific suppression of in vitro T cell responsiveness parallels a selective suppression of anti-hapten but not anti-carrier antibody responses.
Using an antigen of defined physical structure with precisely mapped immunogenic sites, we asked whether those molecular sites previously shown to be critical for immune response gene-mediated initiation of T cell proliferation and T help correspond to the same molecular regions capable of inducing antigen-specific suppression of T cell proliferation and antibody production. Inbred strain 2, 13, and 2 x 13 F1 hybrid guinea pigs were immunized with various species variants or fragments of insulin adjuvant before subsequent immunization with antigen in complete Freund's adjuvant. Analysis of the patterns of depressed T cell responsiveness showed a striking correspondence to the Ir gene-dependent mechanism that controls the recognition of discrete regions within the insulin molecule observed in T cell help in antibody production. In addition, suppression of carrier-specific T cells parallels suppression of anti-hapten antibody responses when hapten is presented on the suppressed carrier without a concomitant suppression of the anti-carrier antibody response.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle; D003433:Crosses, Genetic; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D005620:Freund's Adjuvant / Q000494:pharmacology; D005802:Genes, MHC Class II; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D012756:Sheep; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D002352:Carrier Proteins; D004136:Dinitrobenzenes; D000939:Epitopes; D006241:Haptens; D005620:Freund's Adjuvant; D006433:Hemocyanins