Purification of herpes simplex virus-specified major DNA-binding proteins and detection of antibodies to them in human and rabbit sera.
We purified herpes simplex virus type 1 and 2-specified nonstructural DNA-binding proteins (ICP8 and ICSP 11/12, respectively) from infected Vero cells and applied them in ELISA test to analyse human and rabbit sera. Only a weak immune response to the ICP8 and ICSP 11/12 could be demonstrated following experimental HSV-1 or HSV-2 infections of rabbits. Low levels of ICSP8 antibodies were found also in acute HSV infections of man.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D000956:Antigens, Viral / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D011817:Rabbits; D018139:Simplexvirus / Q000032:analysis* / Q000276:immunology; D014764:Viral Proteins / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins