title: Antigen-specific antibody response induced in cultures of human blood lymphocytes in presence of polyethylene glycol.
abstract: Human peripheral blood lymphocytes respond in vitro to sheep red blood cells (SRBC) with the appearance of numerous specific plaque-forming cells, if the Epstein-Barr virus (EBV) is added to the cultures together with the antigen. However, it was found that when polyethylene glycol (mol. wt. 6000) is included in the culture medium at a final concentration of 4%, antigen alone is able to induce an anti-SRBC response, with clear hemolytic plaques of regular size. Addition of EBV causes a further increase in the antigen-specific antibody response.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes; D004854:Herpesvirus 4, Human; D006736:Horses; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012756:Sheep
chemical_list: D000939:Epitopes; D011092:Polyethylene Glycols