[Secretion into the circulating medium of lipids synthetized by isolated Wistar rat liver perfused with high concentrations of oleic acid and glycerol].
In previously 18-h fasting Wistar rats, the liver is isolated and perfused with [9, 10(-3)H2] oleic acid (346 mumol and [1-14C] glycerol (115 mumol). Then, in a circulating medium, the secretion of triacylglycerols -- synthetized de novo and by esterification of exogenous oleic acid -- and VLDL is inhibited. On the other hand, the secretion of phospholipids is getting away that regulatory process.
D000818:Animals; D002250:Carbon Radioisotopes; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010477:Perfusion; D010743:Phospholipids / Q000096:biosynthesis; D011865:Radioisotope Dilution Technique; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis; D014316:Tritium
D002250:Carbon Radioisotopes; D008055:Lipids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; D014316:Tritium; D019301:Oleic Acid; D005990:Glycerol
Sécrétion dans le milieu circulant des lipides synthétisés par le foie isolé de rt Wistar perfusé par des quantités importantes d'acide oléique et de glycérol.