[Antiviral activity of murine interferons produced by bacterial and animal cell translation of messenger RNA].
Interferon was produced by E. Coli bacteria and animal cell messenger-RNA--interferon translation (mRNA--IF). The activity of the interferon produced by simultaneous mRNA--IF translation in these two cellular systems was, approximately, similar. The interferons translated by bacteria and animal cells inhibited the cytopathic effect, reproduction and plaque-formation of vesicular stomatitis virus, and, to a greater extent, of mouse encephalomyocarditis virus. The virus titration was carried out by the dye-uptake method. The bacteria-translated interferon (BTIF) was more susceptible to the indicator-virus dose variation and had antiviral effect of shorter duration than the virus-induced and animal cell-translated interferon. The BTIF, probably, due to the action of bacterial proteolytic enzymes proved nonstable on storage.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004686:Encephalomyocarditis virus / Q000187:drug effects*; D004926:Escherichia coli / Q000378:metabolism; D007372:Interferons / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009522:Newcastle disease virus / Q000187:drug effects*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*
D012333:RNA, Messenger; D007372:Interferons
Antivirusnaia aktivnost' myshinykh interferonov, poluchennykh v usloviiakh transliatsii informatsionnoĭ RNK dlia interferona bakteriiami i kletkami zhivotnykh.