Human neuropeptide Y, somatostatin and vasopressin precursors identified in cell-free translations of hypothalamic mRNA.
Messenger mRNA has been prepared from post mortem human hypothalami and translated in a cell-free system. Using specific antibodies, biosynthetic precursors have been identified to neuropeptide Y (12 kDa), somatostatin (15 kDa) and vasopressin/neurophysin (19 kDa).
D000328:Adult; D000368:Aged; D000818:Animals; D001127:Arginine Vasopressin; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000302:isolation & purification; D009478:Neuropeptide Y; D009481:Neurophysins; D010121:Oxytocin; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D013004:Somatostatin / Q000235:genetics*; D014667:Vasopressins / Q000235:genetics* / Q000302:isolation & purification
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D009481:Neurophysins; D011498:Protein Precursors; D012333:RNA, Messenger; D014667:Vasopressins; D001127:Arginine Vasopressin; D010121:Oxytocin; D013004:Somatostatin; C042084:preproneuropeptide Y