Interferon effect on collagen and fibronectin distribution in the extracellular matrix of murine sarcoma virus-transformed cells.
The transformation of murine BALB/c embryonic fibroblasts by murine sarcoma virus, Moloney strain, followed by prolonged treatment with murine interferon, resulted in the appearance of a new cell population (MSV-IF+). These MSV-IF+ cells are characterized by the recovery of a normal phenotype, contact inhibition, and lack of colony formation in agar. This phenotypic change of the MSV-IF+ cells is associated to the neosynthesis of a dense fibrous matrix beyond the cell periphery. Ultrastructural studies using peroxidase-labeled antibodies enabled us to localize the extracellular distribution of fibronectin and collagen in the MSV-IF+ cell line, compared to normal BALB/c and murine sarcoma virus-transformed cells. In parallel, a significant increase of collagen and fibronectin deposit in the intercellular space of interferon-treated murine sarcoma virus-transformed cells was observed.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects*; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010641:Phenotype; D009053:Sarcoma Viruses, Murine
D005353:Fibronectins; D003094:Collagen; D007372:Interferons