Synthesis of human fibroblast interferon by E. coli.
A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D007372:Interferons