Iron--dioxygen-dependent changes to the biological activities of bleomycin.
Antitumor antibiotic bleomycin can bind to and degrade DNA, both in vivo and in vitro. This DNA damaging property in vitro can be related to its ability to chelate ferrous ions under aerobic conditions leading to the formation of "active oxygens," which are thought to be directly responsible for the damage. At present, evidence points to the hydroxyl radical formed by an iron-catalyzed Haber-Weiss reaction as the free radical most likely to be involved in this damage. When these same reactions occur in the absence of DNA, the free radicals then damage the bleomycin molecule, resulting in changes to its DNA-degrading activity, antibacterial properties, and chemical composition. Attempts to protect both bleomycin and DNA with a variety of specific and nonspecific scavengers have been unsuccessful, with several even showing pro-oxidant activity towards the iron-dependent damage. Only the metal chelators were effective inhibitors of bleomycin-iron-dependent damage to DNA. The damaged bleomycin lost some 50% of its ability to degrade DNA in vitro. This activity was closely paralleled by a loss in antibacterial activity against two different strains of bacteria.
D000818:Animals; D001761:Bleomycin; D002374:Catalase; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D007501:Iron; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D013950:Thymus Gland
D001761:Bleomycin; D008315:Malondialdehyde; D004247:DNA; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase