title: In vitro nickel binding to mononuclear cells in peripheral blood.
abstract: Mononuclear cells from the peripheral blood of four nickel-sensitive persons and 4 controls bound equal amounts of 63 nickel in vitro. 90--99% (mean 96%) of the 63 nickel was removed from the cells by washing three times every 30 minutes for 120 minutes. For 4 other patients and 4 controls, lymphocyte transformation with autologous nickel-incubated, irradiated cells as the stimulant showed that nickel was bound to the cells in sufficient quantities and was present in a form which could induce lymphocyte transformation in vitro in nickel-sensitive patients.
mesh_terms: D003877:Dermatitis, Contact / Q000097:blood*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D009532:Nickel / Q000097:blood* / Q000494:pharmacology
chemical_list: D009532:Nickel