[Method of increasing the activity of a preparation of human cadaver leukocyte interferon].
A simple method for increasing the activity of human leukocyte interferon is described. It is based on the removal from the native preparation of a presumed inhibitor of antiviral effect followed by condensation of the resulting fractions by thin-canal cells TCF-10 of an Amicon apparatus. With this method of concentration there was no loss of the general initial antiviral activity. Depending on the titer of the initial interferon and the condensation degree, the native interferon was concentrated 32--350-fold. The resulting concentrated interferon preparation is nontoxic, areactogenic, safe in animal trials and may be recommended for trials as a therapeutic-prophylactic means in various viral diseases.
D000818:Animals; D002102:Cadaver; D002642:Chick Embryo; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D007962:Leukocytes; D051379:Mice; D011817:Rabbits; D014461:Ultracentrifugation / Q000379:methods; D014721:Vesicular stomatitis Indiana virus
D007372:Interferons
Sposob povysheniia aktivnosti preparata chelovecheskogo leĭkotsitarnogo kadavernogo interferona.