[Isolation of interferon messenger RNA and the synthesis of the double-stranded DNA product].
The data on preparation of the fraction of "enriched" interferon messenger RNA of chick fibroblasts by fractionation of biologically active RNA molecules by molecular weight are presented. The conditions of enzymatic synthesis and characteristics of the single- and double-stranded DNA product are described. The ways of producing more highly specific messenger RNA and DNA products are discussed.
D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000138:chemical synthesis*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis; D007372:Interferons / Q000032:analysis*; D007700:Kinetics; D011061:Poly A / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification
D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D007372:Interferons; D012194:RNA-Directed DNA Polymerase; D011070:Poly I-C
Vydelenie informatsiomnoĭ RNK interferona i sintez dvunitevogo DNK-produkta.