title: Purification and characterization of recombinant human leukocyte interferon (IFLrA) with monoclonal antibodies.
abstract: Recombinant human leukocyte interferon produced in bacteria (IFLrA) was purified to homogeneity with the use of monoclonal antibodies against leukocyte interferon. The purified interferon exhibited a single band of Mr = approximately 19,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid analysis and the NH2-terminal sequence were consistent with the sequence predicted from the DNA. Some of the purified product contained NH2-terminal methionine; the terminal methionine was removed from the rest of the chains.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D001681:Biological Assay; D004274:DNA, Recombinant; D006801:Humans; D007372:Interferons / Q000235:genetics* / Q000302:isolation & purification; D007962:Leukocytes; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D007372:Interferons