[Immunohistochemical localization of actin and myosin in liver, kidney, stomach, heart and skeletal muscle: a reference to a cytoplasmic actin fibrillar network in liver cells (author's transl)].
For immunohistological localizing of actin and myosin in tissue sections, unfixed cryostate sections of rat liver, kidney, stomach, heart, and tongue as well as isolated bovine skeletal myofibrils were incubated with rabbit antibodies against actin respectively myosin from chicken gizzard and detected with anti-rabbit immunoglobulin labeled with fluorescein isothiocyanate. No remarkable differences were found between the 2 antisera. However the results suggested that a fibrillar cytoplasmic net exist especially in hepatocytes and which contains actin and may connect the cell membrane with the endoplasmic reticulum and the nucleus. The net might have static and dynamic functions.
D000199:Actins / Q000032:analysis*; D000818:Animals; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology*; D008099:Liver / Q000166:cytology*; D009132:Muscles / Q000166:cytology*; D009206:Myocardium / Q000166:cytology*; D009218:Myosins / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling; D013270:Stomach / Q000166:cytology*; D013861:Thiocyanates
D000199:Actins; D005452:Fluoresceins; D013861:Thiocyanates; D009218:Myosins; D016650:Fluorescein-5-isothiocyanate
Immunhistochemische Lokalisation von Aktin und Myosin in Leber, Niere, Magen, Herz und Skeletmuskulatur: Hinweis auf ein cytoplasmatisches Aktin-Fibrillennetz in Leberzellen.