Delayed-type hypersensitivity induced by antigen-pulsed, bone marrow-derived macrophages.
Mouse bone marrow cells, cultured in specifically conditioned medium, were found to be virtually pure macrophages after 7 days. When pulsed with antigen, these cells induced and elicited delayed-type hypersensitivity (DTH) with the same efficiency and major histocompatibility complex restrictions as antigen-pulsed peritoneal exudate cells. Both of these macrophage populations had 20% Ia+ cells, as measured by complement-mediated cytotoxicity. When most of the Ia+ cells were removed, the remaining antigen-pulsed macrophages could not elicit DTH in sensitized mice.
D000626:Aminopeptidases; D000818:Animals; D000941:Antigens; D001854:Bone Marrow Cells; D003602:Cytotoxicity, Immunologic; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D051379:Mice; D010455:Peptides / Q000276:immunology; D013194:Staining and Labeling
D000941:Antigens; D010455:Peptides; D006433:Hemocyanins; D000626:Aminopeptidases