Models of bleomycin interactions with poly(deoxyadenylylthymidylic acid). Fluorescence and proton nuclear magnetic resonance studies of cationic thiazole amides related to bleomycin A2.
The interaction of eight 2-substituted thiazole-4-carboxamides, structurally related to cationic terminus of bleomycin A2, with poly(deoxyadenylylthymidylic acid) [poly(dA-dT)] has been studied by using proton nuclear magnetic resonance and fluorescence spectroscopy. These analogues have been used as probes of the complex formed between the parent drug molecule and poly(dA-dT). Aliphatic substituents on the 2' position of 2,4'-bithiazole derivatives restrict the ability of the aromatic ring system to intercalate in the double-helical form of the polynucleotide. Absence or partial removal of the 2' substituent enhances intercalation of the bithiazole system. The cationic side chain does not appear to be involved in the stabilization of any of these complexes, although it may be necessary for their formation. A 2,4':2',4"-terthiazole derivative shows a substantial degree of intercalation which is accompanied by extensive immobilization of the cationic side chain. This suggests that insertion of the aromatic system into the nucleic acid causes the cationic side chain to be pulled in also. Monothiazole analogues do not appear to bind, indicating that at least two thiazole rings are necessary for binding or that proper spacing between the two side chains on either side of the thiazole system is important for binding. The relation of the interactions of these analogues to the biochemical and biological properties of the parent bleomycins is discussed as is the possible use of these data in the design of synthetic bleomycin derivatives having varying affinities and specificities for DNA.
D001761:Bleomycin / Q000378:metabolism*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011067:Poly dA-dT / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D011089:Polydeoxyribonucleotides; D001761:Bleomycin; D011067:Poly dA-dT