Effect of adriamycin on the polyribosome and messenger-RNA content of rat heart muscle.
The myocardial contents of DNA, protein, total and polyribosomal RNA, messenger RNA (mRNA) and polyribosomes were determined in normal and adriamycin-treated rats. While the myocardial contents of DNA and protein changed only little in adriamycin-treated rats, total and polyribosomal RNA decreased by 23.2% and 35%, mRNA and polyribosomes by 51.6% and 38.7%. Size distribution and protein synthetic activity of the polyribosome preparations obtained remained unchanged in the adriamycin-treated rats. We conclude that the reduced myocardial contents of RNA, mRNA and polyribosomes cause the previously observed impairment of myocardial protein synthesis in adriamycin-treated rats and might be important in the pathogenesis of the adriamycin-cardiomyopathy.
D000818:Animals; D004247:DNA / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D011132:Polyribosomes / Q000187:drug effects*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
D012333:RNA, Messenger; D012313:RNA; D004317:Doxorubicin; D004247:DNA