title: Alteration of neuronal activity in response to cyclic nucleotide agents in Aplysia.
abstract: Responsiveness of Aplysia neurons to agents that affect cyclic nucleotide levels was not limited to neurons exhibiting a spontaneous bursting activity pattern. Analyses of the I-V relationship elicited by triangular current ramps within cells exposed to different agents presumably causing elevated cyclic nucleotide levels showed qualitatively similar alterations. These included an increased slope conductance at more negative potentials, possibly related to anomalous rectification, and the induction of a hysteresis in response to a triangular ramp. The paired metacerebral giant cells showed induction of synchronous bursting when exposed to phosphodiesterase inhibitors, and we examined this phenomenon more closely. Classical methods to inactivate a presynaptic source did not eliminate the induction of synchronous bursting. Intracellular injection of a phosphodiesterase inhibitor into a metacerebral giant cell caused changes in the current-voltage relationship similar to those described above for other cells. Subsequent perfusion with the inhibitor caused an enhancement of these effects and the induction of bursting. The alteration of the current-voltage plot in the metacerebral cells and abdominal ganglion cells is qualitatively similar to that induced in the similarly treated bursting neuron R15, suggesting a similar mechanism of action in both burster and nonburster neurons. The implications of these results for cyclic nucleotide mediation of neuronal events are discussed.
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001048:Aplysia / Q000187:drug effects*; D000242:Cyclic AMP / Q000031:analogs & derivatives; D004594:Electrophysiology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects; D009712:Nucleotides, Cyclic / Q000502:physiology*; D013806:Theophylline / Q000031:analogs & derivatives*
chemical_list: D009712:Nucleotides, Cyclic; D006165:Guanylyl Imidodiphosphate; D013806:Theophylline; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D015056:1-Methyl-3-isobutylxanthine