Cystic fibrosis fibroblasts respond normally to isoproterenol.
Thirteen cystic fibrosis and 12 normal strains of skin fibroblasts obtained from the Institute for Medical Research were compared for their degree of production of cyclic adenosine 3':5'-monophosphate in response to isoproterenol and prostaglandin E1. There were no significant differences in their quantitative responses in content of cyclic AMP at two different times whether these cells were growing exponentially or were already confluent. All strains responded similarly to the presence of two types of phosphodiesterase inhibitor. The averaged initial rates of the response to isoproterenol in exponentially growing cells were also similar for the two sets of strains. Although response differed greatly between strains, the response of each strain was relatively reproducible.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D000242:Cyclic AMP / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D012867:Skin / Q000378:metabolism
D010726:Phosphodiesterase Inhibitors; D011458:Prostaglandins E; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; D000242:Cyclic AMP; D007545:Isoproterenol; D015056:1-Methyl-3-isobutylxanthine