A simple and effective method to assess murine delayed type hypersensitivity to proteins.
Mice sensitized with various protein antigens emulsified in complete Freund's adjuvant by a subcutaneous injection at the base of the tail exhibited both immediate and delayed type hypersensitivities 7-8 days following sensitization. Hypersensitivity was assessed by the degree of footpad swelling elicited by challenge with a heat-aggregated form of the antigen. The swelling response was specific for the antigen used for sensitization and often resulted in a near doubling of footpad thickness. Histological examination is conjunction with studies having passive transfer of serum and adoptive transfer of either T cell-enriched or T cell-depleted preparations indicated that whereas the immediate swelling response was achieved with serum, the delayed swelling response was mediated by T lymphocytes. Adoptive transfer of specific delayed type hypersensitivity was also accomplished with small numbers of lymph node T cells obtained following in vitro enrichment and propagation.
D000818:Animals; D000941:Antigens; D002417:Cattle; D000939:Epitopes; D005260:Female; D006968:Hypersensitivity, Delayed / Q000175:diagnosis* / Q000473:pathology; D007158:Immunologic Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D000941:Antigens; D000939:Epitopes; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin