Uncharged tRNA inhibits guanosine 3',5'-bis (diphosphate) 3'-pyrophosphohydrolase [ppGppase], the spoT gene product, from Escherichia coli.
The spot gene product from Escherichia coli, the guanosine 3',5'-bis(diphosphate) 3'-pyrophos phohydrolase [ppGppase] catalyzes the specific release of pyrophosphate from the 3'-position of guanosine 3',5'-bis(diphosphate) [ppGpp]; this reaction is significantly inhibited in the presence of uncharged tRNAPhe yeast. Little or no inhibition is observed with Phe-tRNAPhe, tRNAPhe-CpCpAoxi-red or ribosomal RNA (16S and 23S).
D003864:Depression, Chemical; D004926:Escherichia coli / Q000235:genetics*; D001277:GTP Pyrophosphokinase / Q000378:metabolism*; D006159:Guanosine Tetraphosphate / Q000037:antagonists & inhibitors; D010770:Phosphotransferases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism / Q000494:pharmacology*; D012331:RNA, Fungal / Q000494:pharmacology*; D012343:RNA, Transfer / Q000378:metabolism / Q000494:pharmacology*
D012329:RNA, Bacterial; D012331:RNA, Fungal; D006159:Guanosine Tetraphosphate; D012343:RNA, Transfer; D010770:Phosphotransferases; D001277:GTP Pyrophosphokinase; C027627:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase; D011755:Pyrophosphatases