Axoplasmic transport of microtubule-associated proteins in the rat sciatic nerve.
32P-ATP was injected into the L5 dorsal root ganglion and axoplasmic transport of the phosphorylate MA proteins 2, microtubule-associated proteins 2, was observed. After the injection of 32P-ATP, the nerve was dissected out at prescribed time intervals and sliced into 5-mm pieces. Each segment was electrophoresed on an SDS-polyacrylamide slab gel and subjected to autoradiography. A protein of 310,000 dalton was transported at a velocity of 6.6-10.6 mm/day in the axon with the electrophoretic mobility identical to that of MA proteins 2, one of the key components associated with the microtubules.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D005727:Ganglia, Spinal / Q000502:physiology*; D007700:Kinetics; D008297:Male; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D012584:Sciatic Nerve / Q000502:physiology*
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D010761:Phosphorus Radioisotopes; D011506:Proteins; D000255:Adenosine Triphosphate