Induction of specific cell-mediated cytoxicity in vitro by means of RNA isolated from allografted mice.
Ribonucleic acid extracts were isolated by cold phenol extraction procedure from lymphoid organs of mice immunized with allogeneic skin grafts. Cultivation of lymphocytes from normal mice with these RNA extracts in vitro led to the induction of specific immunological reactivity which was determined by microcytotoxicity assay. Pretreatment of the RNA extracts with DNase enhanced their ability to induce cytotoxicity, while pronase had no effect, and RNase completely inhibited the ability of extracts to sensitize lymphocytes. The results show that specific cell-mediated cytotoxicity can be induced in vitro in lymphocytes from normal animals by a transfer of "immune" RNA.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D003851:Deoxyribonucleases / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D011402:Pronase / Q000494:pharmacology; D012313:RNA / Q000276:immunology* / Q000302:isolation & purification; D012260:Ribonucleases / Q000494:pharmacology; D016038:Skin Transplantation; D014184:Transplantation, Homologous
C013674:RNA, immune; D012313:RNA; D003851:Deoxyribonucleases; D012260:Ribonucleases; D011402:Pronase