Reactivity of human lymphoid and lymphoblastoid cells with peanut agglutinin: detection of a blood cell subset which lacks detectable membrane HLA.
The number of cells reacting with fluorescein-labelled peanut agglutinin varies widely among human lymphoid organs. By separation techniques, reactive cells from tonsils were characterized as E-negative, non-immunoglobulin-bearing lymphocytes. In peripheral blood, the lectin reacted with a small percentage of lymphoid and monocytoid cells which lacked HLA membrane determinants but expressed the beta 2-microglobulin antigens. The latter phenotype was also found on human lymphoblastoid cell lines. Lectin binding thus provides an effective means of characterizing these cell subsets.
D000937:Antigen-Antibody Reactions; D010367:Arachis; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D000939:Epitopes; D005260:Female; D006680:HLA Antigens; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D037102:Lectins / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D037121:Plant Lectins; D011247:Pregnancy; D011947:Receptors, Antigen, B-Cell; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D007140:Immunoglobulin Fab Fragments; D037102:Lectins; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell