A sensitive assay method for the measurement of S-adenosylmethionine in tissue.
An assay method has been developed for the measurement of tissue levels of S-adenosylmethionine based upon the ability of this compound to activate tripolyphosphatase associated with S-adenosylmethionine synthetase beta prepared from rat liver. The method has been used to measure S-adenosylmethionine levels in rat liver after feeding rats on various concentrations of methionine in the diet. The results obtained by this method agree well with those measured by the spectrophotometric method. The limit of sensitivity of the assay was about 0.1 nmol of S-adenosylmethionine in an incubation volume of 0.1 ml (10(-6) M).
D017766:Acid Anhydride Hydrolases; D000818:Animals; D004032:Diet; D007700:Kinetics; D008099:Liver / Q000032:analysis* / Q000201:enzymology; D008715:Methionine / Q000378:metabolism; D008716:Methionine Adenosyltransferase / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000032:analysis*
D012436:S-Adenosylmethionine; D008715:Methionine; D008716:Methionine Adenosyltransferase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C023193:tripolyphosphatase