Plastic-embedded human marrow biopsy specimens: improved histochemical methods.
Improved methods for processing, sectioning, and staining plastic (glycol methacrylate)-embedded human marrow biopsy specimens were studied. Special stains, including naphthol AS-D-chloro-acetate esterase, PAS, reticulin, and iron, have been modified so that they are suitable for undecalcified, 2-microns-thick, plastic-embedded human marrow biopsy specimens. These adaptations permit plastic-embedded marrow specimens to be used for clinical diagnosis. Marrow biopsy specimens embedded in plastic were compared with biopsy specimens preserved by the conventional paraffin method. The plastic-embedded marrows provide better results from morphologic examination (enhancing diagnostic accuracy), permit assessment of bone as well as of marrow, and allow histochemical analysis to be performed.
D000741:Anemia, Aplastic / Q000473:pathology; D001585:Benzoyl Peroxide / Q000494:pharmacology; D001706:Biopsy / Q000379:methods*; D001853:Bone Marrow / Q000473:pathology; D001856:Bone Marrow Examination / Q000379:methods*; D006652:Histological Techniques; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology; D009101:Multiple Myeloma / Q000473:pathology; D010232:Paraffin; D010969:Plastics; D013194:Staining and Labeling
D008689:Methacrylates; D010969:Plastics; C005044:hydroxyethyl methacrylate; D010232:Paraffin; D001585:Benzoyl Peroxide