Lindane degradation by cell-free extracts of Clostridium rectum.
For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and gamma-3,4,5,6-tetrachlorocyclohexene. Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.
D000596:Amino Acids / Q000494:pharmacology; D002474:Cell-Free System; D003013:Clostridium / Q000378:metabolism*; D003470:Culture Media; D004229:Dithiothreitol / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D006859:Hydrogen / Q000494:pharmacology; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D011773:Pyruvates; D001556:Hexachlorocyclohexane; D006859:Hydrogen; D005947:Glucose; D004229:Dithiothreitol