Responses of Fanconi anemia fibroblasts to adenine and purine analogues.
Fanconi anemia (FA) fibroblasts are known to be exceptionally sensitive to the cytotoxic action of mitomycin C (MMC). The survival of FA cells was enhanced significantly when 0.5 mM caffeine or 0.5 mM adenine was added for 72 h after the cells were exposed to MMC. In other experiments in which MMC was not used, FA fibroblasts were shown to be significantly more sensitive than control cells to 6-mercaptopurine (6-MP), 6-thioguanine (6-TG), and 6-azauridine (6-AU). These observations offer a new approach to defining the basic biochemical defect in FA.
D000741:Anemia, Aplastic / Q000235:genetics*; D001260:Ataxia Telangiectasia / Q000235:genetics; D001380:Azauridine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D015122:Mercaptopurine / Q000494:pharmacology*; D008937:Mitomycins / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D013866:Thioguanine / Q000494:pharmacology*
D008937:Mitomycins; D001380:Azauridine; D015122:Mercaptopurine; D013866:Thioguanine