Pre-stained slides for the demonstration of rosette-forming cells.
Twenty-one specimens of cerebrospinal fluid (CSF) were examined for T lymphocytes (sheep erythrocyte rosette-forming cells, E-RFC). Conventional wet slide preparations and prestained slides were compared. The mean difference between parallel preparations was 2.5%. Prestained slides are convenient for the examination of RFC. Preparations are easily made without any disfigurement of the cells or disruption of rosettes.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D048588:Benzoxazines; D002555:Cerebrospinal Fluid / Q000166:cytology*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D008875:Middle Aged; D010078:Oxazines / Q000494:pharmacology; D012397:Rosette Formation / Q000379:methods*; D012756:Sheep; D013194:Staining and Labeling; D013997:Time Factors
D048588:Benzoxazines; D010078:Oxazines; C028911:cresyl violet; D008751:Methylene Blue