Cytological sub-division of S-phase in the Syrian hamster (Mesocricetus auratus).
Using BrdU/Hoechst 33258/Giemsa methods for detecting replicating chromosome bands, a method is described by which the DNA synthesis phase may be sub-divided on the basis of distinctive patterns displayed by certain chromosomes.--Applied to asynchronous populations successively sampled through one cell cycle, cells in S can be "unscrambled" and replaced in their correct time sequence.--This helps to overcome the sampling-time variable inherent in such populations, and allows a clearer picture of the progression of events both qualitatively and quantitatively.
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle; D002478:Cells, Cultured; D006224:Cricetinae / Q000235:genetics*; D004261:DNA Replication; D005260:Female; D007399:Interphase; D008297:Male; D008647:Mesocricetus / Q000235:genetics*; D008677:Metaphase; D013194:Staining and Labeling
D001973:Bromodeoxyuridine