Concomitant induction of the cell surface expression of Ia determinants and accessory cell function by a murine macrophage tumor cell line.
This study demonstrates that an uncharacterized soluble factor produced in concanavalin A-induced rat spleen cell suspensions has the capacity to induce the increased expression of cell surface H-2K and H-2D molecules and the expression of I-region gene products on murine monocyte-macrophage lineage tumors that are not Ia positive in the absence of the factor. In parallel with induction of serologically defined Ia specificities, Ia-induced WEHI-3 macrophage tumor cells are capable of providing accessory cell function in stimulating IL-2 production by T-T hybridomas that are activated in a major histocompatibility complex-restricted, antigen-dependent fashion. The uninduced Ia-negative WEHI-3 tumor cells do not trigger a comparable response in this assay system.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D002452:Cell Count; D002460:Cell Line; D000939:Epitopes; D005434:Flow Cytometry; D006041:Goats; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II