Public B cell alloantigen determinants (Ia) and active enhancement of renal allografts in the rat.
Experimental renal transplantation in the rat model has established that immunity to certain donor B cell Ia antigenic determinants is associated with enhanced graft survival. Preimmunization of LEW hosts with BUF lymphoid cells produced significant prolongation (P < 0.005 by log rank analysis) of survival of renal allografts taken from a third-party (LEW X BN)F1. Serological analysis demonstrated that BN and BUF share and MHC-encoded public Ia specificity, expressed on B cells, which we have called Ia.15. Hyperimmune sera directed at this specificity are cytotoxic to 20 +/- 3% of lymph node cells, significantly inhibit the formation of EA rosettes by Fc receptor-bearing cells (EAI), and inhibit mixed lymphocyte reaction (MLR) by 50 to 60% when directed at the stimulator but not when directed at the responder strain cells. Study of a large number of hyperimmune sera raised between inbred rat strains, by EAI and complement-dependent cytotoxicity (CDC) versus B cells, has revealed broad patterns of cross-reactivity, suggesting that public Ia determinants are both complex and highly immunogenic. Immunity restricted to a public Ia antigen plays a role in allograft enhancement.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D016030:Kidney Transplantation; D008285:Major Histocompatibility Complex; D051381:Rats; D011919:Rats, Inbred Strains; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera