Endogenous peroxidases: a morphological and functional marker to study inflammatory skin diseases.
The visualization of peroxidase positive lysosomes by diaminobenzidine plus H2O2 allowed easy identification of neutrophils, eosinophils, monocytes and young tissue macrophages in 0.2 micrometer to 0.5 micrometer skin plastic sections of large surface area. Evaluation of the lysosomal functions of these phagocytic cells was greatly improved by the possibility of correlating observations on the same cell at light and electron microscopic levels.
D015100:3,3'-Diaminobenzidine; D003872:Dermatitis / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide; D008247:Lysosomes / Q000201:enzymology; D008854:Microscopy, Electron; D010544:Peroxidases / Q000378:metabolism*; D012867:Skin / Q000201:enzymology* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods
D015100:3,3'-Diaminobenzidine; D006861:Hydrogen Peroxide; D010544:Peroxidases