Early studies in cybrids derived from fusion of mouse lymphosarcoma cells LS/BL and L cell cytoplasts.
This paper describes the proliferative and morphological properties of cybrid cells derived from fusion of the whole cells of a mouse lymphosarcoma LS/BL and cytoplasts prepared from L cells or their biochemically marked mutants (HGPRT-). In cybrids, decondensation of parent LS/BL nuclei and their cooperation with heterologous cytoplasm was observed. Cybrids incorporated the precursors of nucleic acid and protein synthesis up to 70 h after fusion.
D000818:Animals; D002459:Cell Fusion; D003593:Cytoplasm / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D007739:L Cells / Q000502:physiology*; D008228:Lymphoma, Non-Hodgkin / Q000503:physiopathology*; D051379:Mice; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012513:Sarcoma, Experimental / Q000503:physiopathology
D012313:RNA; D004247:DNA