The separation of three antibody populations from anti-poly(A).poly(U) antibodies elicited in mice or rabbits and antigenic features of poly(A).poly(U)).
Anti-poly(A).poly(U) antibodies in ascitic fluid of DDY mice immunized with poly(A).poly(U)-methylated bovine serum albumin complexes were fractionated into three major antibody populations, Ab-1, Ab-2 and Ab-3, by precipitating with poly(I).poly(C), poly(A).poly(U), and poly(A).2 poly(U), respectively. Antibody population one, Ab-2, reacted with various double-stranded RNAs [poly(I).poly(C), poly(A).poly(U), and rice dwarf virus ribonucleic acid (RDV-RNA)] and poly(A).2 poly(U). Ab-2 reacted with poly(A).poly(U) and poly(A).2 poly(U). Although both Ab-1 and Ab-2 reacted with poly(A).poly(U), the two populations were distinguishable by their different reactivities against chemically modified antigens and oligonucleotides. In contrast to Ab-2, acetylation at the furanose 2'-position of poly(U) resulted in a dramatic decrease in the complement fixation reactivity of Ab-2. Also, Ab-2 was capable of binding with complexes of hexa- to heptaadenylates and poly(U), whereas Ab-1 required oligoadenylates of longer chain lengths (9-10 chain length) for binding. Therefore, it appears that poly(A).poly(U) possesses unique antigenic determinants which are recognizable only by Ab-2, in addition to those determinants which are common to a variety of double-stranded RNAs.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000276:immunology; D003168:Complement Fixation Tests; D000939:Epitopes / Q000276:immunology; D051379:Mice; D009690:Nucleic Acid Conformation; D011063:Poly A-U / Q000276:immunology*; D012330:RNA, Double-Stranded / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D000939:Epitopes; D012330:RNA, Double-Stranded; D011063:Poly A-U