Simplified method for DNA and protein staining of human hematopoietic cell samples.
A rapid reproducible method yielding high resolution analysis of DNA and protein in human hematopoietic cell samples has been developed by modification of the propidium iodide and fluorescein isothiocyanate procedure. Cell staining involves sequential addition of each reagent (RNase, fluorescein isothiocyanate and propidium iodide) to ethanol-fixed cells and requires no centrifugation steps. Stained cells are analyzed in the reagent solutions. Analysis of bone marrow samples from multiple myeloma patients showed mixed normal and aneuploid populations with a major portion of the aneuploid cells having a significantly higher protein content. This approach permitted differential cell cycle analysis of normal and the aneuploid populations.
D002453:Cell Cycle; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D011419:Propidium; D011506:Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D013861:Thiocyanates
D005452:Fluoresceins; D011506:Proteins; D013861:Thiocyanates; D011419:Propidium; D004247:DNA; D016650:Fluorescein-5-isothiocyanate