Isolation of rat alpha-fetoprotein from Morris hepatoma 7777.
1. Tissue slices from Morris hepatoma 7777 were incubated for 3 hr in Krebs-Ringer-bicarbonate solution and the cell-free supernatants were used for isolating alpha-fetoprotein. 2. Purification procedure included salting out with ammonium sulphate, affinity chromatography (either Concanavalin A-Sepharose or Cibacron-Blue-Sepharose), hydrophobic chromatography and preparative polyacrylamide gel electrophoresis (PAGE). 3.alpha-Fetoprotein was obtained with a yield of approx. 10% and in pure form, as judged from crossed immunoelectrophoresis and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).
D000818:Animals; D007123:Immunoelectrophoresis, Two-Dimensional; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D051381:Rats; D011915:Rats, Inbred BUF; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D009363:Neoplasm Proteins; D000509:alpha-Fetoproteins