Surface markers of a purified peritoneal eosinophil population from Mesocestoides corti-infected BALB/c male mice.
Eosinophils of approximately 95% purity were prepared from the peritoneal cavities of BALB/c male mice infected with larval cestode, Mesocestoides corti. The alloantigenic surface marker phenotype of this cell population was shown to be H-2+Ly4+Ly5+Lyt-1-,2-,3-Ly-6-,7-Ia-Thy-1-TL-. Two of four anti-Lyt-2 sera were positive when tested on purified eosinophils by using the Staphylococcus aureus protein A sheep erythrocyte rosetting method, but absorption studies indicated that this reaction was not due to anti-Lyt-2 antibodies. Eosinophils are therefore Lyt-2-, although some Lyt-2 sera contain additional eosinophil reactive antibodies. A proportion (20 to 40%) of the population of eosinophils was positive for the Fc receptor, but all were negative for the C3 receptor and for surface immunoglobulin.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002590:Cestode Infections / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera / Q000494:pharmacology; D007519:Isoantigens; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell; D011951:Receptors, Complement; D011961:Receptors, Fc; D012397:Rosette Formation
D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera; D007519:Isoantigens; D011947:Receptors, Antigen, B-Cell; D011951:Receptors, Complement; D011961:Receptors, Fc