Exometabolites of Leishmania donovani promastigotes. II. Spontaneous changes of exometabolite after isolation.
The defined medium of Steiger and Teiger conditioned by growth oif Leishmania domovani strain 3S promastigotes served as the primary source of parasite exometabolites. Four fractions of the conditioned medium were recovered by Sephadex G-25 column chromatography. These fractions shared immunologic determinants, but differed in their molecular weights, affinity of Sephadex G-25, and absorption spectra. Degradation and/or aggregation of one of the fractions (Fraction IV) appeared to yield substances found in the remaining three fractions. Furthermore, by appropriate manipulations, Fraction IV could be transformed into substances resembling those described by previous workers, eg., excretory factor (EF) or antigenically active glycoproteins (AAGP). The present findings suggest that the primary exometabolite released by L. donovani is a samll glycopeptide-like molecule; however, in media conditioned by growth of promastigotes and during isolation procedures, aggregation and/or degradation processes yield higher molecular weight substances which vary in size and physico-chemical properties.
D000818:Animals; D003470:Culture Media; D000939:Epitopes; D006020:Glycopeptides / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D007891:Leishmania / Q000254:growth & development / Q000378:metabolism*; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet
D003470:Culture Media; D000939:Epitopes; D006020:Glycopeptides