Puromycin-induced argyrophilia as an alternative to Golgi methods: reliable impregnations of hypothalamic cell groups following lateral ventricular injections in rats.
Parenchymal injections of puromycin were known to induce Golgi-like silver impregnations upon treatment with the cupric-silver method. We have found lateral ventricular injections (5 microliter, 0.1 M) also effective. Such injections consistently led to detailed Golgi-like profiles of cells in a number of hypothalamic regions including one, the paraventricular nucleus, which traditionally is quite recalcitrant to Golgi methods. It is concluded that this technique is a reliable alternative to Golgi methods for the study of many hypothalamic regions.
D000818:Animals; D003300:Copper; D007031:Hypothalamus / Q000033:anatomy & histology* / Q000166:cytology; D008297:Male; D009474:Neurons / Q000166:cytology; D011691:Puromycin; D051381:Rats; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D011691:Puromycin; D003300:Copper