Analysis of T cell hybridomas. I. Characterization of H-2 and Igh-restricted monoclonal suppressor factors.
Five hybridoma T cell lines were prepared by fusion of second order suppressor T cells (Ts2) with the BW5147 thymoma. The culture supernates from these T cell hybrids contained a factor, TsF2, which specifically suppressed 4-hydroxy-3-nitrophenyl acetyl hapten (NP)-induced cutaneous sensitivity responses. TsF2 activity was observed when the factor was administered during the effector phases of the immune response. TsF2 bears I-J determinants and has binding specificity for NPb idiotypic determinants. TsF2 suppressor activity could be absorbed on antigen-primed H-2-incompatible T cells but cannot suppress H-2-incompatible mice. In addition to this H-2 restriction, which maps to the I-J subregion, monoclonal TsF2 also has an Igh genetic restriction. The present results are combined with previous data to describe the cellular interactions leading to immune suppression.
D000042:Absorption; D000818:Animals; D001666:Binding Sites, Antibody; D002469:Cell Separation; D002874:Chromosome Mapping; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II; D006825:Hybridomas / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic