Comparison of histologic and immunologic heterogeneity of non-Hodgkin's lymphomas.
The lymphocyte surface marker phenotype in 11 selected cases of non-Hodgkin's lymphomas was determined with anti-immunoglobulin and mouse monoclonal antibodies against human lymphocyte antigens. A complement-mediated cell cytotoxicity assay on suspensions of the tumor cells was compared with an indirect immunoperoxidase technique on frozen tissue sections. Both methods gave good results in tumors with a uniform cell population, but the frozen section technique was superior in heterogeneous tumors. The six B-cell neoplasms were heterogeneous with respect to expression of surface immunoglobulin and Ia antigens. The five T-cell tumors were morphologically heterogeneous and also highly variable in their expression of different T-cell specific antigens.
D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D002675:Child, Preschool; D003584:Cytological Techniques; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005629:Frozen Sections; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000473:pathology; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D011947:Receptors, Antigen, B-Cell