Comparison of the haemocytometer and tube modifications of the leucocyte adherence inhibition assay.
The haemocytometer and the tube modifications of the leucocyte adherence inhibition assay were compared in guinea pigs immunized with bovine gamma globulin or bovine serum albumin. In the direct test, both modifications yielded positive results. In the indirect test, which is dependent on a cell-free mediator of the lymphokine nature, the haemocytometer LAI assay only gave positive results. The factor produced by immune guinea pig lymphocytes and responsible for LAI reactions in the haemocytometer modification was also able to inhibit adherence of human and rat leucocytes, but not of mouse leucocytes. The results confirm the earlier suggestion that the tube and haemocytometer LAI assays have a different mechanism.
D000818:Animals; D002417:Cattle; D006168:Guinea Pigs; D007114:Immunization; D007158:Immunologic Techniques; D007957:Leukocyte Adherence Inhibition Test / Q000295:instrumentation; D008722:Methods; D051379:Mice; D051381:Rats; D012710:Serum Albumin, Bovine / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D005719:gamma-Globulins; D012710:Serum Albumin, Bovine