Uveitis, vitreous humor, and klebsiella. I. Binding studies with rabbit antisera.
Uveitis occur in a proportion of patients with ankylosing spondylitis, and an increased faecal isolation of the Gram-negative micro-organism Klebsiella pneumoniae has been reported from such patients. Immunological cross-reactivity between K. pneumoniae and bovine vitreous humour has been studied by 2 different antibody binding techniques: I125-labelled antigen binding assay with and without carrier, and beta-galactosidase enzyme-immunoassay. Sera from rabbits immunised with whole klebsiella micro-organisms or klebsiella extracts were found to bind labelled vitreous humour antigens to a greater extent (p less than 0.001) than sera from rabbits immunised with Escherichia coli, Streptococcus pyogenes, and phi X 174 virus or sera from the same rabbits before immunisation. It is suggested klebsiella micro-organisms may carry antigenic determinants which resemble vitreous humour antigens.
D000818:Animals; D001666:Binding Sites, Antibody; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007711:Klebsiella pneumoniae / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011869:Radioligand Assay; D014606:Uveitis, Anterior / Q000276:immunology; D014822:Vitreous Body / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera