Isolation of human alpha-1 fetoprotein (AFP) from induced abortion material.
The isolation of alpha-1 fetoprotein (AFP) from material of an induced abortion is described. AFP is purified by anion exchange chromatography on DEAE-Sephadex A 50, affinity chromatography on Con-A-Sepharose, and preparative electrophoresis on agarose gel. The yield is about 10% of the original material. The purity was demonstrated by agarose gel electrophoresis and two-dimensional immunoelectrophoresis. The high concentration of AFP in material of induced abortion makes it particularly suitable for deriving human AFP.
D000029:Abortion, Legal; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D011247:Pregnancy; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D000509:alpha-Fetoproteins