title: Syncytium-forming virus of common marmosets (Callithrix jacchus jacchus).
abstract: This communication describes the isolation and characterization of a new syncytium-forming virus of common marmosets (Callithrix jacchus jacchus). The virus, isolated from skin explants and peripheral leukocytes of healthy animals, induced syncytia and subsequent cytolysis of several human, simian, and rodent fibroblastic cultures and induced a carrier state in mixed fibroblastic-epithelial or epithelial cell lines. Cytoplasmic and nuclear viral antigen was demonstrated in infected cells by indirect immunofluorescence tests using serum obtained from persistently infected common marmosets. Abundant virus particles were detected within cisternae of endoplasmic reticulum of lytically infected cells by electron microscopy. The virus incorporated [3H]uridine, banded at a density of 1.14 to 1.16 g/cm3 in sucrose, and possessed ribonucleic acid-dependent deoxyribonucleic acid polymerase. No antigenic cross-reactivity was detected between the marmoset virus and simian foamy virus serotypes 1 to 8 in neutralization and immunofluorescence assays. A seroepidemiological survey of a marmoset colony revealed that 53.5% of common marmosets contained antibodies against the virus, whereas other species of marmosets maintained in the same colony remained free of antibodies.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002143:Callitrichinae / Q000382:microbiology*; D002500:Centrifugation, Isopycnic; D003588:Cytopathogenic Effect, Viral; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000302:isolation & purification*; D013045:Species Specificity; D016092:Spumavirus / Q000276:immunology / Q000302:isolation & purification*; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D012194:RNA-Directed DNA Polymerase