Immunological study of antisera to to artificial O-antigen of Pseudomonas aeruginosa.
O-sera were obtained by immunizing rabbits with artificial antigens: polysaccharide extracted from the lipopolysaccharide of P. aeruginosa (strain No. 868; 03a, 3d, 3e), or the high-molecular-weight or low-molecular-weight fraction of this polysaccharide, complexed with a natural protein (human IgG or rabbit globulin). The antisera to these antigenic complexes were highly O-specific. Antisera to the complexes of polysaccharide-protein and high-molecular-weight fraction-protein were more active in the passive haemagglutination reaction, slide agglutination test and immunodiffusion test in agar gel than was antiserum to the low-molecular-weight fraction-protein complex. The artificial antigens prepared and employed in the study are apt to be used for the preparation of monospecific immune sera.
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000276:immunology*; D000939:Epitopes; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000907:Antibodies, Bacterial; D000939:Epitopes; D007106:Immune Sera; D008070:Lipopolysaccharides