Slow growing Bacillus subtilis: deoxyribonucleic acid replication in turbidostat cultures.
Comparisons of slowly growing Bacillus subtilis cultures grown in batch culture, a chemostat, and a turbidostat were made. Cultures in a turbidostat were found to grow optimally and to be in balanced growth. The duration of deoxyribonucleic acid (DNA) replication, or C period, was estimated by means of an autoradiographic analysis, a genetic analysis, and determinations of DNA per cell. These measurements were performed on cells growing at different slow rates. The results showed that the C period increased with increasing generation times, but the increase in duration of the C period was not proportional to the increase in generation time.
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques / Q000295:instrumentation; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D005819:Genetic Markers; D012329:RNA, Bacterial / Q000096:biosynthesis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005819:Genetic Markers; D012329:RNA, Bacterial