The effect of a sulfated polysaccharide on antithrombin III.
A major naturally occurring inhibitor of blood is AT-III. We have investigated the potentiation of AT-III inhibition of factors Xa, IIa, and plasmin by the heparinoid substance SP-54. Both coagulation and amidolytic methods were used. SP-54 potentiated AT-III inhibition of factors Xa and IIa in the absence of heparin. When heparin was present, potentiation of inhibition of factor Xa usually occurred, but not of factor IIa. SP-54 also potentiated the AT-III inhibition of plasmin action. Laurell immunoelectrophoresis showed no changes in AT-III in the presence of SP-54. In view of the recent importance placed on the role of AT-III and factor Xa in thrombogenesis, an oral agent which potentiates AT-III activity can have important implications for thrombotic therapy.
D000818:Animals; D000990:Antithrombin III / Q000378:metabolism*; D001777:Blood Coagulation / Q000187:drug effects*; D002417:Cattle; D002846:Chromatography, Affinity; D005170:Factor X / Q000378:metabolism; D015951:Factor Xa; D005341:Fibrinolysin / Q000378:metabolism; D006493:Heparin / Q000031:analogs & derivatives / Q000494:pharmacology*; D007122:Immunoelectrophoresis; D010426:Pentosan Sulfuric Polyester / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D011516:Prothrombin / Q000378:metabolism
D011134:Polysaccharides; D010426:Pentosan Sulfuric Polyester; D000990:Antithrombin III; D011516:Prothrombin; D005170:Factor X; C025337:Factor IIa; D006493:Heparin; D015951:Factor Xa; D005341:Fibrinolysin