BALB/c myeloma retroviruses: peptide mapping and immunological analysis of the pp12 structural protein.
We have compared the pp12 structural protein of the MO-21 and FL-1 BALB/c myeloma retroviruses with the pp12 of several prototype retroviruses. Chymotryptic peptide maps of 125I-labeled, immune-precipitated pp12 proteins revealed that the MO-21 and FL-1 proteins can be distinguished from one another. The MO-21 pp12 most closely resembled the NIH-xenotrophic virus pp12, and the FL-1 pp12 most closely resembled the pp12 of BV-2 and WN 1802 B. Competition radioimmunoassay studies showed that the MO-21 and FL-1 pp12 proteins are also antigenically distinct from one another and that both contain pp12 antigenic determinants of a xenotropic virus. These data support our proposal that these two BALB/c viruses contain a gag gene that was generated by recombination between endogenous eco- and xenotropic viral sequences.
D000818:Animals; D000939:Epitopes; D017988:Gammaretrovirus / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis* / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000939:Epitopes; D010455:Peptides; D010750:Phosphoproteins; D014764:Viral Proteins