[Quantitative evaluation of the effectiveness of using Meldola blue and sephadex in the histochemical detection of dehydrogenases in cryostatic sections].
Ther influence of meldola blue (MB), sephadex G-150 and ficoll 400 on kinetics of histochemical reactions for succinate and lactate dehydrogenases in cryostat sections of rat myocardium was estimated spectrophotometrically. MB is an effective electron carrier, although its application in quantitative dehydrogenase histochemistry is limited by its property to bind up with tissues non-enzymatically. 5 x 10(-5) M is the optimal MB concentration in the incubation medium which accelerates the enzymatic reaction in cryostat sections. The advisability of addition of sephadex G-150 to prevent from diffusion of LDH to the incubation medium is confirmed.
D000818:Animals; D003911:Dextrans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006651:Histocytochemistry; D006652:Histological Techniques; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D010078:Oxazines / Q000494:pharmacology*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000032:analysis*
D003911:Dextrans; D010078:Oxazines; C005559:Meldola blue; C025614:sephadex; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase
Kolichestvennaia otsenka éffektivnosti ispol'zovaniia golubogo Mel'doly i sefadeksa pri gistokhimicheskom vyiavlenii degidrogenaz v kriostatnykh srezakh.