Determination of tryptophan and metabolites in rat brain and pineal tissue by reversed-phase high-performance liquid chromatography with electrochemical detection.
Tryptophan and many of its indole metabolites were separated using reversed-phase high-performance liquid chromatography (HPLC) and determined using electrochemical detection. This was accomplished isocratically using an acetate--citric acid eluent with various amounts of methanol. Brain and pineal tissue was analyzed for several tryptophan metabolites. Tissue preparation required only homogenization in acidic solution and centrifugation prior to application to the HPLC column. Detection limits in the low picogram range were found for those indoles separated.
D006916:5-Hydroxytryptophan / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008550:Melatonin / Q000032:analysis; D010870:Pineal Gland / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000032:analysis; D014364:Tryptophan / Q000032:analysis* / Q000378:metabolism
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D006916:5-Hydroxytryptophan; D008550:Melatonin