Deoxyribonucleases of Streptococcus sanguis.
Three DNases, designated 1, 2 and 3 have been isolated from lysates of old (incompetent in genetic transformation) and logarithmic phase (competent) Streptococcus sanguis strain Challis cells. These DNases have different electrophoretic mobilities and immunological properties. Influence of some activators and inhibitors on their activity was determined. Molecular weights of 3 DNases are in the range of 52,000--57,000. All these enzymes are endonucleases which degrade native DNA; thermally denatured DNA is a poor substrate. The enzymes described in this paper differ from the previously known DNases of Streptococcus pyogenes and Streptococcus pneumoniae. Nucleases similar or identical to the Challis strain enzymes have been found in two other S. sanguis strains--Wicky and Blackburn.
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D004573:Electrolytes / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012313:RNA / Q000494:pharmacology; D011817:Rabbits; D013298:Streptococcus sanguis / Q000201:enzymology*
D004573:Electrolytes; D004791:Enzyme Inhibitors; D012313:RNA; D003851:Deoxyribonucleases