Cytochemical localization of glycoconjugates in rat peritoneal mast cells during degranulation.
Cytochemical methods for the localization of glycoconjugates including concanavalin A-horseradish peroxidase (ConA-HRP) and dialysed iron were used to study the distribution of glycoconjugates in mast cell granules during degranulation. The ConA-HRP method revealed intense staining of discharged mast cell granules. Dialysed iron staining was seen at the granule periphery, with extruded granules exhibiting more intense staining than undischarged granules.
D000818:Animals; D003208:Concanavalin A; D005260:Female; D006027:Glycosides / Q000378:metabolism*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D007501:Iron; D008407:Mast Cells / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D013194:Staining and Labeling
D006027:Glycosides; D007202:Indicators and Reagents; D003208:Concanavalin A; D007501:Iron; D006735:Horseradish Peroxidase