Synthesis of the minor fetal hemoglobin Fic in colonies of erythropoietic precursors isolated from human umbilical cord blood.
The biosynthesis of the minor hemoglobin FIc, which contains acetylated gamma chains, and the major hemoglobin Fo was studied during erythroid cell differentiation and maturation in cultures of erythroid precursors isolated from five human umbilical cord blood samples. A gradual decrease in the synthesis of Hb FIc was observed during the maturation of the erythroid cells when cultured from 7 to 15 days. The synthesis of Hb Fo did not show a consistent change; however, the relative synthesis of Hb FIc was the highest in the 7-day-old colonies and decreased at day 15 to levels observed in the reticulocytes. The addition of sodium butyrate, which is known to promote histone acetylation, significantly increased the synthesis of Hb FIc in 7-day-old colonies.
D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006441:Hemoglobin A / Q000096:biosynthesis; D006801:Humans; D012156:Reticulocytes / Q000378:metabolism
D002087:Butyrates; C034605:fetal hemoglobin F(Ic); D020148:Butyric Acid; D006441:Hemoglobin A; D005319:Fetal Hemoglobin