T cell responses to alloantigens. II. In vitro induction of specific Ts by heat-inactivated allogeneic lymphoid cells.
When viable B6AF1 murine spleen cells were cultured in combination with heat-inactivated B10.D2 spleen cells under appropriate conditions, alloantigen-specific suppressor T cells (Ts) were generated. The optimal conditions for generation of specific Ts included a responder cell number of 2 to 4 X 10(6)/ml, a high stimulator to responder ratio, the use of at least 30 min of heat treatment (45 C) of stimulator cells, and 6 or more days of culture.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D000939:Epitopes; D006358:Hot Temperature; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D007519:Isoantigens