Cytotoxic T cell response to minor histocompatibility antigens: apparent lack of H-2 restriction in killers stimulated by membrane fragments.
The secondary cytotoxic T cell response of BALB/c to B10.D2 or DBA/2 minor histocompatibility antigens in vitro requires the participation of an adherent cell. Nylon wool-passed spleen cells were only able to respond to nonadherent intact stimulator cells, or to membrane fragments derived from those cells, if a syngeneic adherent cell were present in the cultures. When the H-2 restriction properties of cytotoxic cells generated in response to various types of stimulation were analyzed, it was found that responses to B10.D2 or DBA/2 intact cells were always H-2 restricted. Responses to syngeneic adherent cells presenting B10.D2 or DBA.2 freeze-thaw antigen were either entirely or predominantly lacking in H-2 restriction as defined by efficient competition by B10 (H-2b) cold target cells. These unrestricted killers appeared to recognize minor histocompatibility as an independent determinant rather than as an H-2d/minors moiety cross-reaction with H-2b, because they were not absorbed by BALB.B (H-2b) macrophage monolayers, but were absorbed by B10 monolayers. Similarly, B10 but not BALB.B cold targets were able to compete for the anti-B10.D2 killers. These experiments eliminate the possibility that the lack of restriction was due to an H-2b restricted receptor cross-reactive with H-2b. Possible models to explain these findings are discussed.
D000818:Animals; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006648:Histocompatibility; D007519:Isoantigens / Q000145:classification / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011948:Receptors, Antigen, T-Cell; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell