Fluorescent microscopy of glycol methacrylate-embedded tissue.
Formalin fixed, glycol methacrylate-embedded tissue samples can easily be used for immunofluorescent studies by direct and indirect fluorescein isothiocyanate staining after treatment with protease V to unmask antigenicity. It is, therefore, possible to use serial 2-micron glycol methacrylate-embedded sections for immunologic studies, as well as conventional and special stains.
D000180:Acrylic Resins; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques; D006801:Humans; D008856:Microscopy, Fluorescence; D009393:Nephritis / Q000175:diagnosis; D011102:Polyhydroxyethyl Methacrylate; D013194:Staining and Labeling
D000180:Acrylic Resins; D011102:Polyhydroxyethyl Methacrylate