Establishment of unresponsiveness in primed B lymphocytes in vivo.
As an approach to examine the influence of the state of cellular activation on the ability to tolerize B cells, the induction of unresponsiveness in human gamma-globulin-(HGG) primed B lymphocytes was studied in an adoptive transfer system. In contrast to transferred normal spleen cells, spleen cells from HGG-primed mice are not readily rendered unresponsive when exposed to the tolerogen, deaggregated HGG (DHGG), in irradiated recipients. A kinetic study showed that unfractionated primed spleen cells do not respond to an antigenic challenge given between 6 and 10 days after cell transfer and injection of DHGG, indicating that they are transiently depressed. In contrast, isolated primed B cells are tolerized when transferred to recipients and treated with DHGG in the absence of T cells. Furthermore, primed B cells exposed to tolerogen in the recipients do not recover the ability to respond to HGG either after a secondary challenge with AHGG given up to 14 days after transfer, or after 2 consecutive challenges given on days 14 and 24 after transfer. The presence of primed T cells at the time of tolerization interferes with the induction of unresponsiveness in these primed B cells. These studies suggest that the presence of primed T cells is responsible for the inability to tolerize unfractionated primed spleen cells populations and that primed B cells themselves are not intrinsically resistant to the induction of unresponsiveness.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D006801:Humans; D007108:Immune Tolerance; D007116:Immunization, Passive; D007700:Kinetics; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D005719:gamma-Globulins