Demonstration of primary cytotoxic T cells in venous blood and cerebrospinal fluid of children with mumps meningitis.
Cryopreserved lymphocytes from venous blood and cerebrospinal fluid (CSF) of 10 children with mumps meningitis were tested in 5-hr 51Cr-release assays against uninfected and mumps virus-infected PHA-blasts. Lymphocytes from all patients were cytotoxic to autologous mumps virus-infected target cells, but completely failed to lyse histoincompatible virus-infected PHA-blasts. Cytotoxicity was specific for the infecting virus, and was mediated by E rosette-forming lymphocytes. The effector cells were present over 2 to 3 wk after onset of meningitis. Mumps viral antigens appeared to be preferentially recognized in association with HLA B determinants. The results show that specifically sensitized cytotoxic T cells (CTL) are induced in patients with mumps meningitis. These cells circulate in venous blood and are locally enriched in CSF. Based on clinical observations, it is proposed that mumps-specific CTL play an immunopathologic role.
D000208:Acute Disease; D000956:Antigens, Viral; D002452:Cell Count; D002555:Cerebrospinal Fluid / Q000166:cytology; D002648:Child; D002675:Child, Preschool; D003289:Convalescence; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D008590:Meningoencephalitis / Q000150:complications* / Q000276:immunology; D009107:Mumps / Q000150:complications* / Q000276:immunology; D009109:Mumps virus / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D014680:Veins
D000956:Antigens, Viral; D000939:Epitopes; D006680:HLA Antigens