Antibodies to cell surface antigens detected by a rapid spot test using ethidium bromide.
We describe here a spot test to detect antibodies to non-erythroid cell surface antigens, using ethidium bromide staining to visualize areas of target cell lysis. This assay allows rapid screening of large numbers of samples of culture supernatants, antisera, or ascitic fluids. Antibody titers determined by this spot test are shown to correlate well with titers determined by two other methods, providing a simple method for initial estimation of the amount of antibody in positive samples.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000954:Antigens, Surface; D000961:Antilymphocyte Serum / Q000032:analysis / Q000494:pharmacology; D001202:Ascitic Fluid / Q000032:analysis / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D004996:Ethidium / Q000494:pharmacology*; D006825:Hybridomas / Q000032:analysis / Q000276:immunology; D007106:Immune Sera / Q000032:analysis; D007158:Immunologic Techniques; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011817:Rabbits; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology; D014343:Trypan Blue / Q000494:pharmacology
D000906:Antibodies; D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D007106:Immune Sera; D004996:Ethidium; D014343:Trypan Blue