[Cultivation of herpesvirus in suspension culture on a microcarrier].
The time course of RK-13 cell growth on a microcarrier DEAE-sephadex A-50 treated by different methods was studied. Treatment of the microcarrier according to the modified method was shown to provide the best conditions for cell attachment and growth. Herpes virus type I in monolayer cultures reached maximum titers at 2--3 days depending on the multiplicity of infection (6.5--7.83 lg CPD50). In cell cultures on the microcarrier at a high multiplicity of infection the virus titer reached maximum values (9.5 lg CPD50) as early as 24 hours postinfection.
D000818:Animals; D002460:Cell Line; D003637:DEAE-Dextran / Q000031:analogs & derivatives*; D003911:Dextrans; D004983:Ethanolamines; D066298:In Vitro Techniques; D007668:Kidney; D011817:Rabbits; D018139:Simplexvirus / Q000254:growth & development*; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods
D003911:Dextrans; D004983:Ethanolamines; C027184:DEAE-Sephadex A-50; D003637:DEAE-Dextran
Kul'tivirovanie virusa gerpesa v suspenzionnoí kul'ture na mikronositele.