Brain (Na+, K+)-ATPase and noradrenergic activity: effects of hyperinnervation and denervation on high-affinity ouabain binding.
To examine the role of nerve-specific (Na+, K+)-ATPase in chronic changes in noradrenergic activity, we examined the effects of noradrenergic denervation and hyperinnervation on p-nitrophenylphosphatase activity and on total and nerve-specific ouabain binding. High-affinity and erythrosin B-sensitive binding were compared as measurements of nerve-specific binding. Hyperinnervation and denervation was produced in cerebellum and cerebral cortex, respectively, by 6-hydroxydopamine lesions of the dorsal noradrenergic bundle. Hyperinnervation increased, and denervation decreased, enzyme activity, high-affinity ouabain inhibition, and erythrosin B-sensitive ouabain binding. As (Na+, K+)-ATPase has a major role in the regulation of neural excitability and energy metabolism, and the ouabain binding site has been shown to have endogenous ligands, these changes in (Na+, K+)-ATPase may be important in the long-term regulation of neuron function by norepinephrine.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002531:Cerebellum / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D003714:Denervation; D004923:Erythrosine / Q000494:pharmacology; D006892:Hydroxydopamines / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000502:physiology*; D010042:Ouabain / Q000378:metabolism / Q000494:pharmacology*; D016627:Oxidopamine; D011485:Protein Binding; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013194:Staining and Labeling
D006892:Hydroxydopamines; D010042:Ouabain; D016627:Oxidopamine; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D004923:Erythrosine; D009638:Norepinephrine