[Effect of adenosine on synthesis of pyrimidine nucleotides and RNA in normal lymphocytes and under conditions of chronic lymphoid leukosis].
Effect of exogenous adenosine on synthesis of pyrimidine nucleotides and RNA was studied in lymphocytes of the patients with leukemic form of chronic lymphoid leukosis. In presence of adenosine lymphocytes were incubated in mixtures containing the precursors--2,3-2(3)H-aspartic and 2-14C-orotic acids as well as 5-3H-uridine. Effect of adenosine on the activity of key enzymes of pyrimidine nucleotide synthesis (orotidine monophosphate pyrophosphorylase and uridine kinase) was studied. The enzymatic activity was estimated using radiochemical procedures in extracts of lymphocytes stored at -20 degrees after acetone drying. Adenosine similarly inhibited synthesis of pyrimidine nucleotides and RNA in normal and leukemic leukocytes. One of the mechanisms, by means of which the inhibitory effect of adenosine was realized, was related to the enzymatic activity of orotidine monophosphate pyrophosphorylase and uridine kinase.
D000241:Adenosine / Q000494:pharmacology*; D001224:Aspartic Acid / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009963:Orotic Acid / Q000378:metabolism; D011742:Pyrimidine Nucleotides / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism
D011742:Pyrimidine Nucleotides; D012334:RNA, Neoplasm; D001224:Aspartic Acid; D009963:Orotic Acid; D012313:RNA; D000241:Adenosine; D014529:Uridine
Vliianie adenozina na sintez pirimidinovykh nukleotidov i RNK v normal'nykh limfots takh i pri khronicheskom limfoleĭkoze.