[Uptake and incorporation of 3H-orotic acid in isolated perfused rat liver].
3H-orotic acid incorporation into RNA and the level of RNA polymerase activity in isolated rat liver perfused for 5 hrs were investigated. In spite of a dramatic decrease in 3H-orotic acid uptake by liver cells during perfusion, a constant rate of RNA synthesis was observed. Moreover, RNA polymerase I and II activities were not affected by a 5-hr perfusion. It is suggested that isolated perfused rat liver can be used to study direct effects of hormones and drugs on RNA synthesis.
D000818:Animals; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009963:Orotic Acid / Q000378:metabolism*; D010477:Perfusion; D012313:RNA / Q000378:metabolism; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D051381:Rats
D009963:Orotic Acid; D012313:RNA; D012319:RNA Polymerase II; D012318:RNA Polymerase I
Assunzione e incorporazione di acido orotico-3H nel fegato isolato e perfuso del ratto.