title: The technique and clinical application of the cytochemical section bioassay for gastrin-like activity.
abstract: The technique and validation of the cytochemical section bioassay for gastrin-like activity are described. This assay utilises the measurement of hormone-induced changes of carbonic anhydrase activity in guinea-pig gastric parietal cells. The clinical use of the assay as identified elevated levels of fasting gastrin-like biological activity in the plasma of patients with duodenal ulcer and has also demonstrated discrepancies between radioimmunoreactive gastrin levels, biological activity, and the clinical presentation of peptic ulcer disease.
mesh_terms: D000328:Adult; D000818:Animals; D007611:Aprotinin / Q000378:metabolism; D004381:Duodenal Ulcer / Q000378:metabolism; D005215:Fasting; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D005755:Gastrins / Q000097:blood*; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D015043:Zollinger-Ellison Syndrome / Q000378:metabolism
chemical_list: D005755:Gastrins; D007611:Aprotinin