title: Isolation and identification of a mannolipid intermediate in the synthesis of pulmonary glycoproteins.
abstract: In order to demonstrate the role of lipid-linked sugars as intermediates in the synthesis of pulmonary glycoproteins, membranous preparations of rabbit lung were incubated with GDP-14C-mannose and selectively extracted with chloroform-methanol (2:1) and chloroform-methanol-water (1:1:0.3). The chloroform-methanol extract was found to contain a lipid-linked sugar which had chemical and chromatographic properties similar to mannosyl-phosphoryl dolichol. The chloroform-methanol-water extract contained an oligosaccharide-lipid and the remaining precipitate contained a labeled protein. Transfer of mannose from the labeled mannolipid to the endogenous protein was demonstrated. These studies indicate a glycolipid intermediate is present in the synthesis of oligosaccharides attached to pulmonary glycoproteins.
mesh_terms: D000818:Animals; D004287:Dolichol Monophosphate Mannose / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism; D011817:Rabbits
chemical_list: D006023:Glycoproteins; D011105:Polyisoprenyl Phosphate Sugars; D011506:Proteins; D006155:Guanosine Diphosphate Mannose; D004287:Dolichol Monophosphate Mannose