A monoclonal antibody recognizing a determinant common to HLA-A3 and A11.
Spleen cells from a mouse immunized with an AML cell expressing HLA-A3 produced a hybridoma secreting an anti-HLA-A3, A11 monoclonal antibody, 26D3. By complement-dependent cytotoxicity at dilutions to 1:10(4) the ascites antibody lysed 13/13 A3, 15/15 A11, and 8 other lymphocytes from a panel of 98 donors. The 26 D3 immunoprecipitated a molecule consisting of subunits of 44,000 and 12,000 daltons. Monoclonality of the antibody was demonstrated by isoelectric focusing and protein A affinity chromatography.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D015791:HLA-A3 Antigen; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D015791:HLA-A3 Antigen; D003165:Complement System Proteins