Human leukocyte interferon-mediated granulopoietic differentiation arrest and its abrogation by lithium carbonate.
Interferon has been shown to inhibit erythropoietic and granulopoietic differentiation. Since lithium carbonate (Li) elevates granulocyte levels in a variety of neutropenic disorders, we investigated the effect of Li on human leukocyte interferon (HLIF)-mediated inhibition of granulopoietic differentiation. Using an agar culture technique for cloning granulocyte-macrophage progenitor cells (GM-CFC), we demonstrated that Li blocks HLIF-induced granulopoietic differentiation arrest in a dose-dependent manner. Results of removal of T lymphocytes from marrow cells suggest that this Li effect is not mediated through marrow T lymphocytes.
D002454:Cell Differentiation / Q000187:drug effects; D002999:Clone Cells / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007962:Leukocytes / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology*; D016651:Lithium Carbonate; D013601:T-Lymphocytes / Q000378:metabolism
D016651:Lithium Carbonate; D007372:Interferons; D008094:Lithium