The variable influence of an alpha component in pregnancy plasma on four different assay systems for the measurement of pregnancy-specific beta1 glycoprotein.
Four methods (radioimmunoassay, electroimmunoassay, laser nephelometry and kinetic immunoturbidimetry) have been evaluated for the measurement of proteins with pregnancy-specific beta1 glycoprotein immunoreactivity. The influence of two maternal plasma proteins with different electrophoretic mobilities (alpha2 and beta1) but with similar antigenic determinants has been assessed in each assay system. The radioimmunoassay method, which utilises a homogeneous preparation of pregnancy-specific beta1 glycoprotein for the preparation of iodinated tracer, and the kinetic immunoturbidimetric assay which measures antigen-antibody complex formation over the first minute of the reaction, were both considerably more specific for the beta1 component of pregnancy specific beta1 glycoprotein immunoreactivity than the laser nephelometric or electroimmunoassay methods.
D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007158:Immunologic Techniques; D007834:Lasers; D009391:Nephelometry and Turbidimetry; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000276:immunology*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000276:immunology*; D011863:Radioimmunoassay
D000939:Epitopes; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins