Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones.
Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in our assay, or their effect may be produced by anti-idiotype. Thus, anti-idiotype may provide two or more specific T cell signals.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology / Q000494:pharmacology; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007376:Interleukin-2