[Incubation of Beef tRNALeu in interferon-treated cells lysate leads to a partial removal of the 3' terminal sequence (author's transl)].
Beef tRNALeu and tRNAArg labeled with [32P]-phosphate at their 3' hydroxyl or 5'-phosphate ends have been incubated in the presence of lysates of interferon-treated Mouse cells. The degradation of the two tRNAs has been followed by polyacrylamide slab gel electrophoresis. In all cases, the incubation leads to a degradation of the tRNAs to a variable extent, but tRNALeu is degraded much faster than tRNAArg. This study, provides evidence that the 3' terminal C - C - A is the primary target of degradation leading to a very fast inactivation of tRNALeu and to the inhibition of translation of exogenous and synthetic mRNAs.
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000502:physiology; D012346:RNA, Transfer, Amino Acyl / Q000502:physiology*
D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D007372:Interferons
L'incubation de tRNALeu de boeuf dans un extrait de cellules traitées par l'interféron conduit à l'enlèvement d'une partie de la séquence 3' terminale.