Adaptation of a low-power stereomicroscope to scan cell colonies labelled with fluorescein isothiocyanate.
A stereomicroscope was modified for use in visualizing fluorescence-labelled cell-colonies at low magnifications. A xenon lamp and an FITC filter (Zeiss) were used to obtain exciting radiation to illuminate fluorescent-labelled specimens. The system is precise enough to distinguish between normal and cancer cell-colonies after specific interaction with FITC-conjugated antiserum.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D008114:Liver Neoplasms, Experimental; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D013861:Thiocyanates; D000509:alpha-Fetoproteins / Q000032:analysis*
D005452:Fluoresceins; D013861:Thiocyanates; D000509:alpha-Fetoproteins; D016650:Fluorescein-5-isothiocyanate