title: Increased production of extracellular enzymes by the synergistic effect of genes introduced into Bacillus subtilis by stepwise transformation.
abstract: The amyR3, amyS, papS1, tmr, and papM118 mutations each stimulate alpha-amylase production two- to sevenfold above the level in a wild-type strain of Bacillus subtilis. A strain which presumably has all five of these mutations produced 250-fold more alpha-amylase.
mesh_terms: D000681:Amylases / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D005809:Genes, Regulator; D014169:Transformation, Bacterial; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D000681:Amylases; D000516:alpha-Amylases