Assay of defective-interfering semliki forest virus by the inhibition of synthesis of virus-specified RNAs.
We describe a simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV) which is based on the inhibition of synthesis of virus-specified RNAs in SFV-infected cells. Using the assay, we have been able to show that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus and we have calculated the u.v. target size of the interfering activity.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003673:Defective Viruses / Q000235:genetics*; D007372:Interferons; D007668:Kidney; D051379:Mice; D012367:RNA, Viral / Q000096:biosynthesis*; D012672:Semliki forest virus / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays; D014762:Viral Interference
D012367:RNA, Viral; D007372:Interferons