Determination of indoles in human and rat pineal.
Tryptophan, serotonin, N-acetylserotonin, melatonin, 5-hydroxyindoleacetic acid and 5-hydroxytryptophol have been determined in rat and human pineal glands. The compounds were measured by directly injecting centrifuged tissue homogenates into a liquid chromatographic-fluorometric system. Normal ranges are reported for these compounds and upper limits established for several other indoles.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D006917:Hydroxytryptophol / Q000032:analysis; D007211:Indoles / Q000032:analysis*; D008550:Melatonin / Q000032:analysis; D010870:Pineal Gland / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000031:analogs & derivatives / Q000032:analysis; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis
D007211:Indoles; D006917:Hydroxytryptophol; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D008550:Melatonin; C006389:N-acetylserotonin