Antigen-specific T lymphocyte clones. I. Characterization of a T lymphocyte clone expressing antigen-specific suppressive activity.
We have generated continuously propagatable T lymphocyte clones to study antigen-specific T cell functions. All Ly-2+ clones mediate suppressive activity and secrete a characteristic pattern of polypeptides that differs from Ly-2- T cell clones. Cells of one clone, Cl.Ly23/4, specifically bind glycophorin from sheep erythrocytes (SRBC). After incubation with [35S]methionine, supernate material from this clone also contains biosynthetically labeled 70,000-mol wt proteins that specifically bind to SRBC and this binding is inhibited by glycophorin from sheep but not other erythrocytes. These antigen-binding 70,000-mol wt peptides specifically and completely suppress primary anti-SRBC responses generated by mixtures of primed Ly-1+2- cells and B cells. Suppression by these antigen-binding peptides reflects direct inhibition of T-helper activity.
D000818:Animals; D000941:Antigens / Q000378:metabolism; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006021:Glycophorins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D006021:Glycophorins; D010455:Peptides