Indirect alkylation of CBA mouse liver DNA and RNA by hydrazine in vivo. A possible mechanism of action as a carcinogen.
Treatment of inbred CBA mice with hydrazine sulfate, a liver carcinogen, plus [14C]formate or L-[methyl-14C]-methionine as a source of "C1" units, produced [14C]7-methylguanine (7-MG) in liver DNA and RNA. This methylated guanine was identified by its chromatographic characteristics in three different systems: on Sephadex G10, on Dowex 50, and by paper co-chromatography with authentic 7-MG. Mice that received [14C]formate alone showed no 7-MG in their DNA or RNA. In addition, mice treated with L-[methyl-14C]methionine alone showed no 7-MG in their DNA. These results suggest that "indirect" alkylation of nucleic acids is a mechanism of cancer initiation by hydrazine.
D000477:Alkylating Agents; D000818:Animals; D002273:Carcinogens; D004247:DNA / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006834:Hydrazines / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008745:Methylation; D051379:Mice; D008808:Mice, Inbred CBA; D012313:RNA / Q000378:metabolism*
D000477:Alkylating Agents; D002273:Carcinogens; D006834:Hydrazines; D006147:Guanine; D012313:RNA; C008450:7-methylguanine; D004247:DNA