title: Hydrophobic compounds interfere in radioimmunoassay for basic protein in myelin.
abstract: Hydrophobic compounds influenced the accuracy of the radioimmunoassay for myelin basic protein when lipids (stearic acid, phosphatidylcholine, cholesterol, cerebroside, sulfatide, or GM1 ganglioside) or proteolipids (white-matter proteolipid apoprotein, kidney proteolipid apoproteins, or heart proteolipid apoproteins) were added to a known amount of basic protein and the samples assayed. All of these interfere with the assay, but the direction of the error depends on the quantity added: low concentrations of lipid decrease apparent basic protein, high concentrations enhance it. Obviously, results of basic-protein assays must be interpreted carefully.
mesh_terms: D000818:Animals; D001059:Apoproteins; D002417:Cattle; D002554:Cerebrosides; D002784:Cholesterol; D005677:G(M1) Ganglioside; D004676:Myelin Basic Protein / Q000032:analysis*; D010713:Phosphatidylcholines; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013229:Stearic Acids; D013433:Sulfoglycosphingolipids
chemical_list: D001059:Apoproteins; D002554:Cerebrosides; D004676:Myelin Basic Protein; D010713:Phosphatidylcholines; D013229:Stearic Acids; D013433:Sulfoglycosphingolipids; D005677:G(M1) Ganglioside; D002784:Cholesterol