title: [Gel electrophoresis of the alpha-2-Gc globulin group under various conditions].
abstract: Our experiments confirm the transformation of the group-specific component Gc into alpha 1-globulin. This transformation was more marked in blood samples subjected to repeated freezing-thawing procedures, and was very weak in samples subjected to repeated freezing-thawing procedures after mixing serum and red blood cells. The active factor responsible for this transformation was inhibited by heating at 56 degrees C for one minute in a water-bath. The transformation of the group-specific component was accomplished immediately by mixing serum and extract of platelets. The extract of platelets induced an increase in the rate of migration of all Gc components on immunoelectrophoresis, and it induced a decrease in the isoelectric point of all Gc components on Ampholine PAG plates with a pH range of 4 to 6.5. It induced a decrease in the rate of migration of all Gc components on polyacrylamide gel (7.5%) electrophoresis. A similar transformation was induced by homogenized fluid of cardiac muscle of cow and chicken, and pectoral muscle of the chicken.
mesh_terms: D000510:Alpha-Globulins / Q000378:metabolism*; D000818:Animals; D001790:Blood Physiological Phenomena; D001792:Blood Platelets / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D006321:Heart / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D014020:Tissue Extracts; D014807:Vitamin D / Q000378:metabolism*; D014809:Vitamin D-Binding Protein
chemical_list: D000510:Alpha-Globulins; D002352:Carrier Proteins; D014020:Tissue Extracts; D014809:Vitamin D-Binding Protein; D014807:Vitamin D
vernacular_title: Immunoelectrophorese sur gelose du groupe des alpha 2GC-globulines dans diverses conditions.