A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.
A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.
D000469:Alkaline Phosphatase; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D003208:Concanavalin A; D004285:Dogs; D000882:Haplorhini; D006651:Histocytochemistry; D006801:Humans; D008853:Microscopy / Q000379:methods; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D013194:Staining and Labeling; D013552:Swine
D002241:Carbohydrates; D003208:Concanavalin A; D000469:Alkaline Phosphatase