Isolation and properties of isolated nuclei from the Florida red tide dinoflagellate Gymnodinium breve (Davis).
A simple and rapid method is described for the isolation of nuclei from the Florida red tide dinoflagellate Gymnodinium breve. The nuclei are free of cytoplasmic contamination and are active in endogenous RNA synthesis. The ratio of DNA : RNA : acid-soluble protein : acid-insoluble protein is 1:0.39:0.13:0.63, respectively, and each nucleus contains ca. 113 picograms of DNA. Electrophoretic analysis of the acid-soluble proteins reveals the presence of two histone-like proteins with molecular weights of 12,000 and 13,000.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D004141:Dinoflagellida / Q000235:genetics* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis; D012313:RNA / Q000032:analysis*
D006657:Histones; D009698:Nucleoproteins; D012313:RNA; D004247:DNA