The murine B cell response to TNP-polyacrylamide beads: the relationship between the epitope density of the antigen and the requirements for T cell help and surface IgD.
Spleen cells were cultured with high or low epitope TNP-polyacrylamide beads (TNP-PAB) in order to investigate the effect of epitope density on the requirements for T cell help and surface IgD on responding B cells. The response to low epitope density TNP-PAB was abolished by treatment with anti-Thy-1.2 and complement, whereas approximately 50% of the response to high epitope density TNP-PAB was retained after similar treatment. Thus, an increase in epitope density resulted in a decreased requirement for T cell help. An increase in epitope density was also associated with a decreased requirement for interaction of antigen and surface IgD as determined by "blocking" studies with anti-delta; further, the majority of the T-independent portion of this response was not blocked by anti-delta antibodies. This finding indicate that the T-independent portion of the anti-TNP response does not require interaction of antigen with surface IgD on B cells. These results are discussed in terms of differential cross-linking of IgM and IgD receptors on B cells by multivalent antigens.
D000178:Acrylamides / Q000276:immunology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005260:Female; D007072:Immunoglobulin D; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000276:immunology*; D010641:Phenotype; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*
D000178:Acrylamides; D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007072:Immunoglobulin D; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D009578:Nitrobenzenes; D011947:Receptors, Antigen, B-Cell; D014301:Trinitrobenzenes