A protein fraction (NHG) from serum of normal humans which is cytotoxic for HeLa cells in culture.
A fraction of the alpha-globulins (NHG) from normal human serum was cytotoxic for mouse L-cells in culture and Meth A tumors in mice. NHG inhibited the growth in vitro of human colon cancer (HT-29), melanoma (RPMI 7931) and a neuroblastoma cell line. Survival of HeLa S-3 cell colonies after 24 h exposure to 25, 50, 75 or 100 micrograms NHG/ml medium was 86%, 77%, 40% and 10%, respectively. Whole human serum or purified serum albumin had no anti-HeLa cell activity. These results confirm the presence of a protein in human serum with antitumor activity. An assay for NHG using HeLa S-3 tumor cells is described.
D000510:Alpha-Globulins / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008970:Molecular Weight
D000510:Alpha-Globulins; D000970:Antineoplastic Agents