In vitro effects of aflatoxin B1 on the activities of three hydrolases.
The in vitro effects of aflatoxin B1 on the activities of trypsin, amylase and lipase in the pancreatic extract of male albino Wistar rats have been studied. Activities of the 3 hydrolases were inhibited at 1 microgram/ml, 200 microgram/ml and 400 microgram/ml concentrations of aflatoxin B1, with the exception that at the 1 microgram/ml concentration the activity of lipase was stimulated. Lineweaver-Burk reciprocal plots indicate non-competitive inhibition of the 3 hydrolases.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000681:Amylases / Q000037:antagonists & inhibitors; D000818:Animals; D006867:Hydrolases / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008049:Lipase / Q000037:antagonists & inhibitors; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014361:Trypsin Inhibitors / Q000494:pharmacology
D000348:Aflatoxins; D014361:Trypsin Inhibitors; D016604:Aflatoxin B1; D006867:Hydrolases; D008049:Lipase; D000681:Amylases