Kinetics of human immune interferon production and blastogenesis.
We studied some of the requirements for production of immune interferon (IFN-gamma) by human peripheral leucocytes following stimulation with concanavalin A (Con A). Synthesis of IFN-gamma commenced 2 hr after the cells were exposed to the lectin and continued for about 20 hr thereafter. IFN-gamma production during the latter period was independent of free Con A in the culture medium but it was sustained by continued Con A binding and required active de novo protein synthesis. Egg lecithin, which inhibits the blastogenic response, did not affect IFN-gamma production.
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis*; D010713:Phosphatidylcholines / Q000494:pharmacology
D008222:Lymphokines; D010713:Phosphatidylcholines; D003208:Concanavalin A; D007372:Interferons; D003513:Cycloheximide