The effects of Haemophilus influenzae vaccination on anaphylactic mediator release and isoprenaline-induced inhibition of mediator release.
The influence of Haemophilus influenzae on anaphylactic mediator release from ovalbumin-sensitized isolated guinea pig lungs was investigated. Lungs from H. influenzae-vaccinated animals released prostaglandins and thromboxanes following a smaller dose of ovalbumin than was effective in non-vaccinated animals. Histamine release was significantly increased in 4 day-vaccinated animals but not 1 or 10 days after vaccination, while broncho-constriction was potentiated in 1 and in 4 day-vaccinated animals. This increased histamine release was achieved following 2 micrograms ovalbumin. In contrast, doses of 10 micrograms and 1 mg ovalbumin respectively did not affect and decreased histamine release in the vaccinated group. The inhibition of anaphylactic mediator release by an infusion of 6 x 10(-9) M isoprenaline was significantly attenuated by H. influenzae vaccination. These results indicate an increased sensitivity to antigenic challenge and suggest that the functioning of beta-adrenoceptors was decreased as a result of H. influenzae vaccination.
D000707:Anaphylaxis / Q000378:metabolism*; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001249:Asthma / Q000209:etiology; D012898:Autacoids / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology; D001986:Bronchial Spasm / Q000139:chemically induced; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006193:Haemophilus influenzae / Q000276:immunology*; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008168:Lung / Q000276:immunology / Q000378:metabolism*; D008297:Male; D010047:Ovalbumin / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013931:Thromboxanes / Q000378:metabolism; D014611:Vaccination
D001095:Arachidonic Acids; D012898:Autacoids; D011453:Prostaglandins; D013931:Thromboxanes; D006632:Histamine; D010047:Ovalbumin; D007545:Isoproterenol; D001920:Bradykinin