I-J restriction of T cells that suppress in vivo antibody responses to Thy-1.
The contact-sensitizing haptens dinitrophenyl (DNP) and oxazalone (Ox) act as helper determinants for antibody responses to Thy-1 when conjugated to donor thymus cells. The helper effect is transferrable from primed to naive mice with spleen cells, producing specific augmentation of in vivo PFC responses to Thy-1. The helper cells are hapten-specific and require associative recognition of hapten and Thy-1, excluding a role for nonspecific B cell activation. The phenotype of the helper cells is Thy-1+ and Lyt-1+2-. Antigen-specific suppression could be readily generated by using an inoculum of DNP-modified syngeneic RBC. T cells from these suppressed donors (Ts) were shown to abolish the helper effects of TH in adoptive transfer experiments in vivo. These Ts were characterized as Thy-1+ and Lyt-1-2+. A requirement for MHC compatibility at the I-J subregion was necessary between the Ts and the recipient to obtain a transfer of suppression.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000145:classification / Q000235:genetics*; D007108:Immune Tolerance; D007518:Isoantibodies / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D010081:Oxazolone / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D004136:Dinitrobenzenes; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies; D018800:Thy-1 Antigens; D010081:Oxazolone