Interferon action: RNA cleavage pattern of a (2'-5')oligoadenylate--dependent endonuclease.
One of the mediators of interferon action is a latent endoribonuclease (ribonuclease L) that is activated by (2'-5')oligoadenylates. Among the homopolymers of the four common ribonucleotides, activated ribonuclease L degrades at an appreciable rate only polyuridylic acid. In two natural RNA's tested the most frequent ribonuclease L cleavages occur after UA, UG, and UU (A, adenine; U, uracil; and G, guanine) and much less frequent cleavages after CA and AC (C, cytosine).
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D004722:Endoribonucleases; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D012313:RNA; D012260:Ribonucleases / Q000378:metabolism*; D012265:Ribonucleotides / Q000032:analysis; D013379:Substrate Specificity
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012265:Ribonucleotides; C023505:2',5'-oligoadenylate; D012313:RNA; D007372:Interferons; D004722:Endoribonucleases; D012260:Ribonucleases; C031956:2-5A-dependent ribonuclease