Analysis of some cytotopochemical parameters for a definition of the chromatin status.
A study of the curves of Feulgen hydrolysis kinetics has been performed on different cell types of the same animal species and on the same cell type from different species at different taxonomical level. The curves were analyzed by a computer programme of least square fit to the Bateman's function, which gives a description of DNA hydrolysis kinetics by three parameters only: 1) amount potentially stainable groups; 2) depurination rate constant; 3) depolymerization rate constant. The chromatin homogeneity within the nucleus seems to affect these parameters, together with the degree of chromatin compaction. Furthermore the thermal denaturation (100 degrees C), also after fractionate histone extraction, shows a resistant DNA fraction, which does not appear to be affected by the degree of chromatin compaction only.
D000818:Animals; D002531:Cerebellum / Q000032:analysis; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis; D006652:Histological Techniques; D006657:Histones; D007700:Kinetics; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D009691:Nucleic Acid Denaturation; D011689:Purkinje Cells / Q000032:analysis; D012394:Rosaniline Dyes; D013094:Spermatozoa / Q000032:analysis; D013194:Staining and Labeling
D002843:Chromatin; D004396:Coloring Agents; C007722:Feulgen stain; D006657:Histones; D012394:Rosaniline Dyes; D004247:DNA