Reactivity of antistreptococcus hyperimmune sera with human myocardial tissue.
Antimyocardial antibodies were determined in hyperimmune rabbit sera against group A streptococci. The detection method was indirect immunofluorescence in human myocardial tissue sections. Positively reacting sera were absorbed with group A streptococcus cell walls and cytoplasmic membranes, the objective being to locate the cross-reacting antigen in the streptococcus cell under the given experimental conditions. Cell walls gave the better absorption results. Whether or not the streptococci possessed the Fc-reacting factor was without influence on the absorption result.
D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009206:Myocardium / Q000276:immunology*; D011961:Receptors, Fc; D013297:Streptococcus pyogenes / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D007106:Immune Sera; D011961:Receptors, Fc