[Cultivation of primary animal cells on microcarriers of various types].
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead particles within 38--48 hours when seeded in a dose of 3 x 10(5) cells/ml and on DEAE-Sephadex A-50 within 60--72 hours when seeded in a dose of 4 x 10(5) cells/ml. In both instances, with microcarriers used in a concentration of 1 mg/ml the number of CEC by the end of the cultivation period reached 0.9 million cells/ml. Primary NPK and GMK cells formed monolayers not on all microcarrier particles, nevertheless the number of cells in both cultures increased 2--2 1/2-fold in 7 days. The optimal seed concentration of cells depended both on the quality of the primary cell suspension and on the microcarrier type. The monolayer of CEC cells formed on DEAE-Sephadex A-50 particles persisted for 7--14 days.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003637:DEAE-Dextran / Q000031:analogs & derivatives*; D003911:Dextrans; D004983:Ethanolamines; D000882:Haplorhini; D066298:In Vitro Techniques; D007668:Kidney / Q000196:embryology; D008722:Methods; D013552:Swine
D003911:Dextrans; D004983:Ethanolamines; C027184:DEAE-Sephadex A-50; D003637:DEAE-Dextran
Vyrashchivanie pervichnykh kletok zhivotnykh na mikronositeliakh razlichnogo tipa.