The effect of culture medium composition and incubation time on synthesis of gibberellin-like substances by bacteria isolated from the roots of pine seedlings (Pinus silvestris L.).
It was found that synthesis of gibberellin-like substances by ten strains of Coryneform bacteria isolated from the roots of pine seedlings depended on both the composition of the medium and incubation time. More of these substances were produced in mineral medium with glucose in complex medium with casamino acids and yeast extract. Most gibberellin-like substances were found in 7 or 14-day old cultures. Culture supernatant fluids of most of the bacteria tested contained several gibberellin-like substances which on chromatograms run with the solvent system benzene, acetic acid (10:3, v/v) were located at Rf 0.0-0.3; 0.4-0.6 and 0.8-1.0.
D000192:Actinomycetales / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D003470:Culture Media; D005875:Gibberellins / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D012988:Soil Microbiology; D013045:Species Specificity; D014197:Trees / Q000382:microbiology*
D000596:Amino Acids; D003470:Culture Media; D005875:Gibberellins; D005947:Glucose