Molecular evidence of viral-like biochemical activities in human genitourinary malignancies.
We have demonstrated previously that core structures of urine samples from patients with genitourinary malignancies contain ribonucleic acid-directed deoxyribonucleic acid polymerase and a high molecular weight ribonucleic acid. If these particles originated from the existing genitourinary malignancies then the malignancies should contain similar characteristics. We examined 13 prostatic carcinomas, 4 bladder carcinomas, 1 urethral carcinoma and 1 hypernephroma. Positive reactions were noted in 10 of the 13 prostatic carcinomas (77 per cent), all 4 bladder carcinomas, the 1 urethral carcinoma and the hypernephroma with the simultaneous detection assay. The control samples consisted of 7 tissues of benign prostatic hypertrophy, and tissue from 2 normal bladders and 1 normal kidney. None of these tissues showed a positive response. Tritium labeled deoxyribonucleic acid probes synthesized from the malignant tissues hybridized to the polysomal ribonucleic acids but not to the corresponding normal tissues. Particles derived from the probes have a density of 1,1620 in sucrose gradient. No sequence homology could be demonstrated with various known oncogenic ribonucleic acid viruses nor with malignancies arising from other organs.
D000230:Adenocarcinoma / Q000032:analysis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000032:analysis; D008297:Male; D009693:Nucleic Acid Hybridization; D009858:Oncogenic Viruses / Q000378:metabolism; D011467:Prostate / Q000032:analysis; D011471:Prostatic Neoplasms / Q000032:analysis; D012328:RNA Viruses / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000652:urine; D012194:RNA-Directed DNA Polymerase / Q000032:analysis* / Q000652:urine; D014523:Urethral Neoplasms / Q000032:analysis; D001743:Urinary Bladder / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000032:analysis; D014565:Urogenital Neoplasms / Q000032:analysis* / Q000652:urine
D004273:DNA, Neoplasm; D004279:DNA, Viral; D012334:RNA, Neoplasm; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase