Glutamine synthetase induction in chick embryo retina monolayers.
Induction of glutamine synthetase (GS) by cortisol has been shown to occur in monolayer cultures of cells obtained by enzymatic dissociation of retinas from 8- and 12-day-old chick embryos with papain (0.1%) or trypsin (0.25%). Although essentially single cells when plated, monolayers obtained by enzymatic dissociation show significant aggregation by 4--6 h. Monolayers prepared by mechanical dispersion (cells forced through successively smaller gage needles) are minimally inducible, perhaps owing to poor viability in such cultures. Storage at 4 degrees C for 24 h prior to treatment with cortisol significantly elevated both basal GS activity and inducibility in whole (but not in monolayer) retina cultures.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003080:Cold Temperature; D004790:Enzyme Induction; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology; D010206:Papain / Q000378:metabolism; D012160:Retina / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism
D014357:Trypsin; D010206:Papain; D005974:Glutamate-Ammonia Ligase; D006854:Hydrocortisone