Preparation of resealed pig lymphocyte plasma-membrane vesicles.
Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60--80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt. 5200) and Dextran T10 (mol.wt. 10 000).
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002463:Cell Membrane Permeability; D002499:Centrifugation, Density Gradient; D003911:Dextrans; D007444:Inulin / Q000378:metabolism; D008214:Lymphocytes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008954:Models, Biological; D013552:Swine
D003911:Dextrans; D007444:Inulin