[Comparison of triglyceride and phospholipid synthesis in isolated Wistar rat liver perfused with high quantities of oleic acid and glycerol].
In a recirculating system, [9,10(-3)H2] oleic acid (346 mumol) and [1-14C] glycerol (115 mumol) are perfused into livers of 18-h fasting Wistar rats. These precursors are incorporated in same amounts into triacylglycerols, and in amounts growing up with the duration of the experiment (5 to 120 min). Their incorporation is slight into phospholipids. However, during the experiment, the increase of 3H/14C ratio of every acylglycerols shows that more lipids are synthetized in the acylation way than in the de novo way. The only synthesis of phospholipids, studied in the two ways, seems to be regulated, unlike the one of triacylglycerols in those experimental conditions.
D000215:Acylation; D000818:Animals; D002250:Carbon Radioisotopes; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010477:Perfusion; D010743:Phospholipids / Q000096:biosynthesis*; D011865:Radioisotope Dilution Technique; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis*; D014316:Tritium
D002250:Carbon Radioisotopes; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; D014316:Tritium; D019301:Oleic Acid; D005990:Glycerol
Etude comparée de la synthèse des triacylglycérols et des phospholipides par le foie isolé de rat Wistar perfusé par des quantités élevées d'acide oléique et de glycérol.