[Foetomaternal transfusions after amniocentesis in early pregnancy - evaluation of 1,000 cases (author's transl)].
The foetal haemoglobin (HbF) content was determined before and 2 hours after amniocentesis in 1,000 cases of amniocentesis conditioned by genetic indication (between the 15th and 20th pregnancy week). HbF-identification was effected according to the method of Kleinhauer and Betke. In primarily (before amniocentesis) negative HbF findings, HbF positive findings after amniocentesis were obtained in 7.2% of the patients. However, if the HbF-determinations were positive already before amniocentesis, a quantitative increase of HbF-cells in the maternal blood smear after amniocentesis was observed in 15.9% of the patients. Patients with punctures in the case of placenta of the anterior wall showed and increase in HbF values after amniocentesis in 12.2% of the cases, compared against 8.9% only in patients with posterior wall placenta, but these differences were statistically not significant. Amniocentesis under ultrasonic visual control caused a drop in the number of repeated amniocentesis trials to 20/500, aspiration of bloodstained amniotic fluid to 3.6% and HbF-cell positive blood smears after amniocentesis to 7%. (Free-hand-needle-technique: amniocentesis failures 42/500, amniotic fluid samples contaminated with blood in 9.6% of the cases and increases in HbF-values after amniocentesis in 12.6% of the cases). A total of 82 Rh-negative patients were given conventional anti-D-prophylaxis independent of the localisation of placenta. No sensitization was observed in any of the cases. No boosting was caused by early amniocentesis in cases where a positive anti-body finding had been established (3 cases to date).
D000649:Amniocentesis / Q000009:adverse effects*; D005069:Evaluation Studies as Topic; D005260:Female; D005319:Fetal Hemoglobin / Q000032:analysis; D005323:Fetal Monitoring; D005331:Fetomaternal Transfusion / Q000209:etiology*; D006801:Humans; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D014463:Ultrasonography
D012204:Rh-Hr Blood-Group System; D005319:Fetal Hemoglobin
Feto - maternelle Transfusionen nach Amniocentese in der Frühschwangerschaft, eine Auswertung von 1000 Fällen.