Homologous 3'-terminal regions of mRNAs for surface antigens of different antigenic variants of Trypanosoma brucei.
Sequences corresponding to the complete 3'-terminal regions of the messenger RNAs for three different Variant Surface Glycoproteins of Trypanosoma brucei were determined on complementary DNA inserts cloned in recombinant plasmids. The three sequences show 80-130 base pair long segments of strong (70-80%) homology at the 3' ends, whereas ther regions upstream from the last 130-140 base pairs contain no significant homology. The signal AAUAAA, present near the 3' ends of almost all known polyadenylated mRNAs of eukaryotes, does not occur in the 3'-terminal sequences of these three variants.
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D000939:Epitopes / Q000235:genetics*; D014644:Genetic Variation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000276:immunology*
D000954:Antigens, Surface; D004274:DNA, Recombinant; D000939:Epitopes; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes