Hydrolysis of galactose from glycolipids and glycoprotein by young rat brain beta-galactosidases immobilized to Sepharose 4B.
An extract of glycosidic enzymes from young rat brain was immobilized to cyanogen bromide-activated Sepharose 4B. Most glycosidases retained approximately 10--25% of their activities after immobilization. Immobilized beta-galactosidases were used repeatedly without detectable loss of enzyme activity in the hydrolysis of p-nitrophenyl-beta-D-galactopyranoside. In addition to the synthetic substrate, the immobilized rat brain beta-galactosidases could also hydrolyze galactose from lactose, galactosylcerebroside, asialofetuin, and GM1-ganglioside. The hydrolysis of GM1- to GM2-ganglioside was confirmed on TLC.
D000818:Animals; D001212:Asialoglycoproteins; D001921:Brain / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D060748:Fetuins; D005677:G(M1) Ganglioside / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism*; D005699:Galactosylceramides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D009598:Nitrophenylgalactosides / Q000378:metabolism; D051381:Rats; D012685:Sepharose; D000509:alpha-Fetoproteins / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism*
D001212:Asialoglycoproteins; D004800:Enzymes, Immobilized; D060748:Fetuins; D005699:Galactosylceramides; D000509:alpha-Fetoproteins; C017029:asialofetuin; D009598:Nitrophenylgalactosides; D005677:G(M1) Ganglioside; D012685:Sepharose; D005696:Galactosidases; D001616:beta-Galactosidase; D007785:Lactose; D005690:Galactose