Fusion of small unilamellar liposomes with phospholipid planar bilayer membranes and large single-bilayer vesicles.
Small unilamellar phosphatidylserine/phosphatidylcholine liposomes incubated on one side of planar phosphatidylserine bilayer membranes induced fluctuations and a sharp increase in the membrane conductance when the Ca2+ concentration was increased to a threshold of 3--5 mM in 100 mM NaCl, pH 7.4. Under the same ionic conditions, these liposomes fused with large (0.2 micrometer diameter) single-bilayer phosphatidylserine vesicles, as shown by a fluorescence assay for the mixing of internal aqueous contents of the two vesicle populations. The conductance behavior of the planar membranes was interpreted to be a consequence of the structural rearrangement of phospholipids during individual fusion events and the incorporation of domains of phosphatidylcholine into the Ca2+-complexed phosphatidylserine membrane. The small vesicles did not aggregate or fuse with one another at these Ca2+ concentrations, but fused preferentially with the phosphatidylserine membrane, analogous to simple exocytosis in biological membranes. Phosphatidylserine vesicles containing gramicidin A as a probe interacted with the planar membranes upon raising the Ca2+ concentration from 0.9 to 1.2 mM, as detected by an abrupt increase in the membrane conductance. In parallel experiments, these vesicles were shown to fuse with the large phosphatidylserine liposomes at the same Ca2+ concentration.
D000818:Animals; D001921:Brain; D002118:Calcium; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004553:Electric Conductivity; D006096:Gramicidin; D007700:Kinetics; D008051:Lipid Bilayers; D008081:Liposomes; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D014634:Valinomycin
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D006096:Gramicidin; D014634:Valinomycin; D002118:Calcium