Regulation of allergic reaction by aerobic Corynebacterium equi extract, CEF. III. Inhibition of histamine release from rat peritoneal mast cells.
Effects of a water soluble fraction of Corynebacterium equi (CEF) on the histamine release from rat peritoneal mast cells (RPMC) were investigated. The treatment of RPMC with CEF resulted in a significant inhibition of the histamine release from RPMC which was induced by IgE antibody-antigen interaction as well as nonimmunological histamine liberators. CEF was much more effective in inhibition of IgE-mediated histamine release and compound 48/80 or polymyxin B-induced histamine release when the mast cells were treated with CEF prior to the incubation with the antigen or the liberators. In contrast, CEF was equally effective in inhibition of the histamine release by concanavalin A with phosphatidyl serine when the mast cells were treated with CEF prior to or simultaneously with the liberator. Evidence was also presented that CEF inhibits degranulation of mesenteric mast cells induced by immunological as well as nonimmunological mechanisms.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001202:Ascitic Fluid / Q000166:cytology; D003208:Concanavalin A / Q000494:pharmacology; D003352:Corynebacterium / Q000276:immunology*; D006636:Histamine Release; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D008643:Mesentery / Q000166:cytology; D010718:Phosphatidylserines / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D051381:Rats; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D010718:Phosphatidylserines; D003208:Concanavalin A; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D011112:Polymyxin B