Intracellular ribonucleoprotein complexes of visna virus are infectious.
Sheep choroid plexus cells infected with visna virus produce intracytoplasmic viral ribonucleoprotein complexes with sedimentation values of 120S to 200S and buoyant densities of 1.29 to 1.32 g/cm3. These ribonucleoprotein complexes display an endogenous RNA-directed DNA polymerase activity and contain all of the species of RNA associated with polysomes. An analysis of the polypeptides present in the ribonucleoproteins allowed us to identify the mature internal virion core proteins and their precursor, Pr55gag, as well as the glycosylated envelope precursor gPr150env and small amounts of mature glycoprotein gp135. Ultracentrifugation-purified ribonucleoproteins could infect sheep choroid plexus cells and led to a normal lytic cycle with virus production. Our results suggest that visna virus can propagate by means of intracellular infectious particles.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000502:physiology*; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012261:Ribonucleoproteins / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D012756:Sheep; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D014790:Visna-maedi virus / Q000032:analysis / Q000254:growth & development*
D009698:Nucleoproteins; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase