[Activation of RNA synthesis in loach embryos after injection of a nuclear extract obtained by using 0.35 M NaCl].
An extract mainly containing chromatin nonhistone proteins was obtained by means of 0.35 M NaCl from nuclei isolated from loach (Misgurnus fossilis L.) embryos at the 18 hour developmental stage (late gastrula). Injection of a concentrated nuclear extract into the loach eggs was followed by intensified (1.5--2.0 fold) incorporation of radioactive precursors [3H]uridine or 14CO2 into RNA. The stage at which natural activation of the RNA synthesis occurs (6 hours, mid blastula) remains unchanged, but the rate of incorporation after the onset of synthesis activation (8 hours, late blastula) becomes greater.
D000818:Animals; D001756:Blastoderm / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005399:Fishes / Q000196:embryology*; D005775:Gastrula / Q000032:analysis; D010063:Ovum / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects*; D014529:Uridine / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D002245:Carbon Dioxide; D012313:RNA; D014529:Uridine
Aktivatsiia sinteza RNK v zarodyshakh v'iuna posle in"ektsii ékstrakta iader, poluchennogo s pomoshch'iu 0.35 M NaCl.