On chromatin availability to hydrolysis of the Feulgen reaction in Purkinje neuron population: microdensitometric and microfluorometric data.
The earlier finding that the chromatin organization of Purkinje neurons is not the same in the whole population is confirmed in this study. This was found to be independent of the type of Feulgen reaction hydrolysis kinetics, analytical method (microdensitometry or microfluorometry) and tissue preparation (sections, isolated cells, isolated nuclei). This heterogeneity, possibly linked to different functional stages, leads to Feulgen-DNA contents which, at optimal hydrolysis times, range from 2c to 4c values. The results obtained are discussed in relation to those in the literature.
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004396:Coloring Agents; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D003720:Densitometry; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D008856:Microscopy, Fluorescence; D011689:Purkinje Cells / Q000378:metabolism*; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D002843:Chromatin; D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA