title: Ultrastructure of extracellular matrix of embryonic chick limb bud cartilage.
abstract: The ultrastructure of the extracellular matrix of chick limb buds at stages 24--36 was investigated with the electron microscope and with polarization microscopic evaluation of topo-optical reactions. Two types of extracellular matrix structures could be distinguished. In the primary matrix of presumptive cartilaginous areas at developmental stage 25, topo-optical reactions revealed polycarboxylated glycosaminoglycans oriented parallel to the cell surface. With the electron microscope few fine filaments and small matrix granules could be found. In the secondary cartilage matrix, from stage 26 on, topo-optical reactions demonstrated glycosaminoglycans and collagen being predominantly ordered parallel to each other. Their amount and the degree of their orientation gradually increased during the developmental period investigated. Electron microscopy demonstrated collagen filaments and matrix granules in increasing number at stages 26--36. While some parallel ordering of filaments was seen in the electron micrographs, no sign of spatial ordering of glycosaminoglycans could be found with this technique.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000196:embryology / Q000648:ultrastructure*; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis; D005121:Extremities / Q000196:embryology; D006025:Glycosaminoglycans / Q000032:analysis; D008854:Microscopy, Electron; D013194:Staining and Labeling
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen