An autoradiographic method of detecting A. laidlawii and M. hyorhinis in cell cultures.
The autoradiographic investigation of L cells and chinese hamster cells for the presence of mycoplasmas (A. laidlawii and M. hyorhinis) using uridine/uracil (UdR/U) testing is a rapid and reliable method suitable for the serial checking of even a small number of cells. It depends on a reduced incorporation of [3H]uridine and an increased uptake of [3H]uracil into the RNA of mycoplasma-infected cells, shown in autoradiograms by the density of the grains and their distribution. Results obtained by the autoradiographic technique correspond approximately to specific activity values of RNA-infected cells after the incorporation of [3H]uridine and [3H]uracil.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D007739:L Cells / Q000382:microbiology; D008168:Lung; D051379:Mice; D009174:Mycoplasma / Q000302:isolation & purification*; D012313:RNA / Q000378:metabolism
D012313:RNA