Reduction of [3H]muscimol binding sites in rat dorsal spinal cord after neonatal capsaicin treatment.
Two-day-old rats were pretreated with 50 mg/kg of capsaicin. After 3--4 months, specific binding of [3H]muscimol and [3H]strychnine was measured in membrane preparations from dorsal spinal cord. A 20-30% decrease of the number of [3H]muscimol binding sites was observed after capsaicin treatment. In contrast, [3H]strychnine binding was unchanged. The results provide indirect evidence for a presynaptic location of GABA receptors on capsaicin-sensitive primary afferent neurons.
D000818:Animals; D000831:Animals, Newborn; D002211:Capsaicin / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects*; D009118:Muscimol / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects; D010080:Oxazoles / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011963:Receptors, GABA-A; D012964:Sodium / Q000378:metabolism; D013331:Strychnine / Q000378:metabolism; D013373:Substance P / Q000378:metabolism
D005231:Fatty Acids, Unsaturated; D010080:Oxazoles; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D009118:Muscimol; D013373:Substance P; D012964:Sodium; D013331:Strychnine; D002211:Capsaicin