[Pepsin pretreatment of human semen as a standard procedure for flow cytometry].
The 30 minutes pepsin incubation of human semen has proved to be a standard pretreatment procedure for impuls cytophotometry (ICP). This method, in comparison to papain treatment, shows a better preservation of the material and avoids the phenomenon of "disintegration" and reduced staining property. The CV of 1C peak is small enough. Additionally, the immature spermatozoa, earlier dissociated late elongated spermatids, diploid spermatozoa, dual forms and diploid semen cells (spermatogenetic cells, leukocytes, epithelial cells are demonstrated in small peaks, a fact which can help explain the disturbances in pathological cases.
D004171:Diploidy; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D010206:Papain; D010434:Pepsin A; D012661:Semen / Q000166:cytology*; D013087:Spermatids / Q000166:cytology; D013094:Spermatozoa / Q000166:cytology; D013194:Staining and Labeling; D014646:Varicocele / Q000473:pathology
D010206:Papain; D010434:Pepsin A
Die Pepsinvorbehandlung menschlichen Spermas als Standardmethode in der impulscytophotometrischen Analyse.