title: A charcoal cartridge for the removal of anionic detergent and electrophoresis stains.
abstract: Instructions are given for the construction of a charcoal-containing cartridge that allows the rapid recirculation through charcoal of any fluid in which the cartridge is submerged; recirculatory flow is achieved by magnetic stirring of the fluid by a stirring bar placed under the cartridge. The device is assembled from nylon mesh and conical sections cut from polypropylene beakers. The device can be used to accelerate the destaining of electrophoresis gels and to remove SDS (sodium lauryl sulfate) from SDS gels. The removal of SDS prior to staining is essential for the staining of SDS gels with Coomassie Brilliant Blue G-250.
mesh_terms: D002606:Charcoal; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000295:instrumentation
chemical_list: D012394:Rosaniline Dyes; D002606:Charcoal; D012967:Sodium Dodecyl Sulfate; C004692:coomassie Brilliant Blue