Transfer and expression of herpes simplex virus thymidine kinase and human globin genes in mammalian cells studied by spot hybridization assays.
A spot hybridization assay has been developed for rapid estimations of specific DNA sequences in whole mammalian cells or DNA. This method is applicable to gene-transfer studies in determining the copy number(s) of foreign DNA(s) introduced into mammalian cells. A similar assay has been developed for determining amounts of specific RNA sequences in mammalian cells. It is also shown that this method is applicable to gene-transfer studies and in particular to determining the expression of foreign genes introduced into cells.
D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D005914:Globins / Q000235:genetics*; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics*; D014170:Transformation, Genetic
D012313:RNA; D005914:Globins; D004247:DNA; D013937:Thymidine Kinase