Isolation of mutants of Caulobacter crescentus deficient in ATP-dependent deoxyribonuclease activity.
Caulobacter crescentus mutants sensitive to UV radiation, mitomycin C and methyl methane-sulfonate were isolated and tested for ATP-dependent deoxyribonuclease activity. Two mutants were identified of which one had less than 5 per cent of the wild-type level of the nuclease activity. This mutant in cross with another auxotrophic partner gave highly reproducible two-fold reduction in number of recombinants compared to a control cross. The suggested causes for reduced recombination frequency when one of the partners has residual ATP-dependent deoxyribonuclease activity are discussed.
D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases / Q000172:deficiency; D004352:Drug Resistance, Microbial; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011548:Pseudomonadaceae / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D008937:Mitomycins; D000255:Adenosine Triphosphate; D008741:Methyl Methanesulfonate; D003851:Deoxyribonucleases