A possible mechanism by which SV40 T-antigen stimulates rRNA synthesis.
Binding of SV40 T-antigen to RNA polymerase I could account for the observation that T-antigen stimulates rRNA synthesis and that nucleoli do not stain for T-antigen. Two tests were performed to detect binding: a) RNA polymerase I was isolated and assayed in the presence of T-antigen; polymerase activity was enhanced. b) RNA polymerase I and T-antigen were mixed and then T-antigen complement fixation assays performed, complement fixation was not inhibited.
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D003168:Complement Fixation Tests; D004306:Dose-Response Relationship, Immunologic; D004789:Enzyme Activation; D000939:Epitopes; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000939:Epitopes; D012335:RNA, Ribosomal; D012319:RNA Polymerase II; D012318:RNA Polymerase I