[Electron microscopic demonstration of pulmonary surfactant proteins using procion brilliant blue H5GS].
A new electron microscopy technique is described for detection of lung surfactant proteins with the copper-containing phthalocyanine dye, procion brilliant blue H5GS. The protein structures were stained concurrently with the fixation during perfusion through the pulmonary artery of a fixative-staining mixture containing glutaric aldehyde and a dye in the kakodilate buffer, pH 5.6-6, and in the course of a subsequent immersion of lung tissue pieces into the same mixture. Then the material was treated with thiosemicarbazide and post-fixed with OsO4. The dye did not penetrate intact cells. The electron-dense products of the histochemical reaction were located inside and on the surface of the surfactant membrane, in the hypophase of the surfactant complex, on the plasmalemma of air-blood barrier cells and in its micropinocytosis vesicles, as well as on the membranes of osmophilic plate-like bodies as their contents egressed into the alveolar lumen.
D000818:Animals; D000873:Anthracenes; D006168:Guinea Pigs; D006651:Histocytochemistry; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D014227:Triazines
D000873:Anthracenes; D011663:Pulmonary Surfactants; D014227:Triazines; C032467:procion brilliant blue HGR
Elektronno-mikroskopicheskoe vyiavlenie belkov legochnogo surfaktanta s pomoshch'iu protsionovogo iarko-golubogo H5GS.