title: [Immunochemical characterization of proteolytic enzymes (BAPAase) from buckwheat and rye seeds].
abstract: A comparative study of the immunochemical properties of two serine proteolytic enzymes (BAPases) from buckwheat and rye seeds hydrolyzing N alpha-benzoyl-DL-arginine-p-nitroanilide (BAPA) has been performed. It has been shown that buckwheat and rye seed BAPAases were partially antigenically identical. This was demonstrated using double immunodiffusion in agar and inhibition of one enzyme with an antiserum to the other. At the same time neither of the antisera inhibited trypsin. Thus, buckwheat and rye seed BAPAases have common antigenic determinants and, consequently, common structural features. On the other hand, these enzymes probably have no common structural elements with trypsin.
mesh_terms: D001586:Benzoylarginine Nitroanilide / Q000378:metabolism; D002523:Edible Grain / Q000201:enzymology; D000939:Epitopes; D005779:Immunodiffusion; D010447:Peptide Hydrolases / Q000032:analysis* / Q000276:immunology; D012639:Seeds / Q000201:enzymology*; D014357:Trypsin
chemical_list: D000939:Epitopes; D001586:Benzoylarginine Nitroanilide; D010447:Peptide Hydrolases; D014357:Trypsin
vernacular_title: Immunokhimicheskaia kharakteristika proteoliticheskikh fermentov (bapaaz) iz semian grechikhi i rzhi.