[Radioimmunoassay for type II amino-terminal procollagen peptide. Technical note].
We have studied the immunoreactivity of human sera and synovial fluids in a radioimmunoassay which utilizes an anti-bovine procollagen type III antiserum and the bovine type III procollagen peptide as standard and tracer. We have noticed a complete cross reaction between bovine col 1-3 and human synovial fluid, which thus contains substance (s) having the same antigenic determinant (s) as col 1-3. On the contrary, we have demonstrated non parallelism between col 1-3 and human serum, whether the serum was obtained from normal subjects or patients suffering from various diseases. These results suggest that immunoreactive substances related to col 1-3 and detected in sera are at least partially different from those detected in synovial fluids.
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood; D010001:Osteitis Deformans / Q000097:blood; D010455:Peptides / Q000032:analysis*; D011347:Procollagen / Q000097:blood / Q000302:isolation & purification; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D013045:Species Specificity; D013582:Synovial Fluid
D000939:Epitopes; D010455:Peptides; D011347:Procollagen
Le dosage radioimmunologique du peptide amino-terminal du procollagène de type III. Note technique.