Synthesis of carboxyl terminal segments of beta-subunit of human chorionic gonadotropin. Application of the two-phase method.
The synthesis in solution of carboxyl terminal peptide segments of the beta-subunit of human chorionic gonadotropin is described. The protected segments include sequences 119-131, 132-137, and 138-145. The syntheses were based on a standardized liquid-liquid extraction program for routine purification of intermediates (two-phase method). Condensation of terminally deblocked segments afforded protection peptides 132-145, 126-145, 120-145, and 119-145. Protected peptides 126-145 and 120-145 were deprotected in liquid hydrogen fluoride and used in conjugated form for immunization of rabbits. Data on the specificity of the antibody response are reported.
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D006063:Chorionic Gonadotropin / Q000138:chemical synthesis* / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits
D006063:Chorionic Gonadotropin; D000939:Epitopes; D009842:Oligopeptides; D010446:Peptide Fragments