Mutagenicity of HPLC fractions from extracts of AAtrex-treated corn.
Crude extracts from maize plants treated with varying concentrations of AAtrex, an s-triazine herbicide, were tested for the induction of gene conversion in a diploid strain of Saccharomyces cerevisiae heteroallelic at two loci. These extracts were then subjected to analysis by higher pressure liquid chromatography (HPLC), and the fractions collected in water, 50% methanol, and 100% methanol were similarly tested. Dose-dependent convertogenic activity observed in the 50% methanol fractions suggested that the mutagen(s) is recovered in this fraction.
D001280:Atrazine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism; D005785:Gene Conversion / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D009153:Mutagens / Q000302:isolation & purification*; D010936:Plant Extracts / Q000633:toxicity*; D012313:RNA / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism* / Q000633:toxicity
D009153:Mutagens; D010936:Plant Extracts; D012313:RNA; D004247:DNA; D001280:Atrazine