[Preparation of giant RNA from bovine thyroid gland by agarose gel filtration].
Giant RNA's were prepared by a rapid gel-filtration technique. DNAase, pronase and thyroid homogenate were successively placed on the column. Proteins and DNA were degraded during filtration. Void volume contained pure giant RNA's. Giant RNAs' yield was 7 times higher with this method than with phenol method.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D003851:Deoxyribonucleases; D008970:Molecular Weight; D011402:Pronase; D012313:RNA / Q000302:isolation & purification*; D012685:Sepharose; D013961:Thyroid Gland / Q000032:analysis*
D012313:RNA; D012685:Sepharose; D003851:Deoxyribonucleases; D011402:Pronase
Préparation des RNA géants de la glande thyroïde de boeuf par filtration sur gel d' agarose.