Immunohistochemical localization of substance P and ACTH-like activity in the central nervous system of the earthworm Lumbricus terrestris L.
The peroxidase-antiperoxidase (PAP) method was used for the immunohistological demonstration of substances P and ACTH in the cerebral and subesophageal ganglia of the earthworm, Lumbricus terrestris L. With rabbit antibody to substance P a positive immunoreaction was found in nerve cells smaller than the type A neurons of the cerebral ganglion. Their perikarya and nerve processes as well as their terminal enlargements in the synaptic zone were immunoreactive. ACTH-like activity was visible in a larger perikaryon type of the A neurons. Their nerve processes did not show any reaction. However, a part of the nerve terminals of the synaptic zone and a few neurons of type B of the cerebral ganglion, further some cells of the subesophageal ganglion reacted positively.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D005724:Ganglia / Q000032:analysis; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000032:analysis*; D009835:Oligochaeta / Q000032:analysis* / Q000166:cytology; D013373:Substance P / Q000032:analysis*; D013569:Synapses / Q000032:analysis
D013373:Substance P; D000324:Adrenocorticotropic Hormone