[Causes of decreased enzyme induction after long-term hormone administration: cortisol receptors in the rat liver cells].
The results are presented of experimental studies of hepatic cytosol, nucleus and chromatin hormone-receptor activity of animals with decreased enzyme induction of gluconeogenesis, resultant from prolonged cortisol injection. In such animals in vivo 3H-cortisol incorporation into hepatocyte subcellular fractions was changed, being reduced in cytosol and enhanced in microsomes, nuclei and chromatin. 3H-Cortisol incorporation into chromatin was the highest in combination of the experimental rat hepatic nuclei with cytosol of intact animals after in vitro incubation with reconstructed homogenate labelled hormone from hepatocyte nuclei and cytosol. After incubation of experimental and intact rat nuclei with cytosol of the animals under study 3H-cortisol binding in chromatin was 30% lower than after incubating the nuclei with cytosol from intact rats.
D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D003864:Depression, Chemical; D004790:Enzyme Induction / Q000187:drug effects; D005938:Glucocorticoids / Q000008:administration & dosage; D006854:Hydrocortisone / Q000008:administration & dosage* / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D010088:Oxidoreductases / Q000096:biosynthesis*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013997:Time Factors
D005938:Glucocorticoids; D011956:Receptors, Cell Surface; D010088:Oxidoreductases; D006854:Hydrocortisone
Issledovanie prichin snizheniia induktsii fermentov pri dlitel'nom vvedenii gormona: retseptsiia kortizola v kletkakh pecheni krys.