Production of monoclonal mouse IgE antibodies with DNP specificity by hybrid cell lines.
IgE anti-DNP antibody-secreting hybridomas were obtained by polyethylene glycol fusion of murine myeloma cells with spleen cells from mice, primed with DNP-KLH and challenged with DNP-Nippostrongylus brasiliensis extract. The in vivo and in vitro continuously growing hybridomas are producing high amounts of monoclonal IgE anti-DNP antibodies, which are heat-labile, hapten-specific and have rat mast cell sensitizing capacity.
D000818:Animals; D000918:Antibody Specificity; D004140:Dinitrophenols / Q000276:immunology*; D006636:Histamine Release; D006822:Hybrid Cells / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D013154:Spleen / Q000276:immunology
D004140:Dinitrophenols; D007073:Immunoglobulin E