title: Idiotype-bearing peripheral blood lymphocytes in human multiple myeloma and Waldenström's macroglobulinaemia.
abstract: Antisera raised against idiotypic determinants (ID) of myeloma proteins and macroglobulins have been used to differentiate peripheral blood lymphocyte (PBL) populations from individual patients. ID-positive lymphocytes not resembling plasma cells have been regularly found in peripheral blood in these diseases. For further characterization ID-positive lymphocytes were enriched from the peripheral blood by affinity chromatography using heterologous anti-idiotypic sera. Two patients with IgG myeloma, one patient with Waldenström's macroglobulinaemia and two persons with benign monoclonal hyperglobulinaemia (BMH) were examined by this technique. The ID-positive PBL population was shown to be heterogeneous with respect to non-tumour-specific surface markers, such as sheep erythrocytes (SRBC), Fc and C receptors. Tumour-specific idiotypic determinants will thus allow a more correct recognition of the total tumour cell compartment in these diseases.
mesh_terms: D000328:Adult; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000302:isolation & purification; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M / Q000302:isolation & purification; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology*; D011961:Receptors, Fc; D012397:Rosette Formation; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology*
chemical_list: D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D011961:Receptors, Fc