title: [Electron microscopic study of endopolyploid nuclei in rat trophoblast giant cells. IV. Changes in nucleolar ultrastructure during cell differentiation].
abstract: The ultrastructure of the nucleolus of highly differentiated trophoblast giant cells has been studied on the 17th day of the foetus development. Changes in its morphology have been followed in relation to the degree of nuclear chromatin condensation and to the cell differentiation level. The nucleoli have a reticular structure in the nuclei with dispersed and condensed chromatin. In both the cases the nucleoli involve the four components: fibro-granular, fibrillar (of moderate and normal density) and lacunar regions; fibrillar centres are distinguished within the regions. In the nucleoli with condensed chromatin, unlike those with dispersed chromatin, the perinuclear chromatin is clearly seen, and the penetration of nucleolus-organizer threads along lacunae and deep into the nucleolus can be easily followed. The fibrillar centres are more obvious. With the run of a progressive differentiation of the trophoblast cells, the number of granules is reduced; first, the fibro-granular component covers a significant part of the nucleolus, then granules become visible only in the cortical zone of the nucleolus; in the nuclei with strongly condensed chromatin no granules are seen in the nucleolus.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002466:Cell Nucleolus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D008854:Microscopy, Electron; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D012313:RNA
vernacular_title: Elektronno-mikroskopicheskoe issledovanie éndopoliploidnykh iader v gigantskikh kletkakh trofoblasta krysy. IV. Izmenenie ul'trastruktury iadryshka v khode differentsirovki kletok.