[Effect of treatment with Triton X-100 on DNA integrity and DNA:RNA: protein ratio of rat liver nuclei].
A procedure is described that gives clean nuclei with intact DNA from a rat liver cell suspension. Cytoplasmic contamination is removed by successive treatments with a non ionic detergent, Triton X-100 (0.75%, v/v). We found that with ratios DNA:RNA: protein of 1:0.09:3.29 (2 Triton X-100 steps) the integrity of DNA is preserved: further decrease in RNA and protein content (3 Triton X-100 steps) causes DNA breakage, probably because of extraction of nuclear proteins. In order to estimate DNA integrity, its viscosity was determined by the use of a new oscillating crucible viscometer; this method makes possible the evaluation of extremely small levels of DNA damage as that induced by 0.067 mg/kg of DMNA injected i.p.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D008297:Male; D009698:Nucleoproteins / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014783:Viscosity
D009698:Nucleoproteins; D011092:Polyethylene Glycols; D012313:RNA; D017830:Octoxynol; D004247:DNA
Effetto del trattamento con Triton X-100 sull'integrità del DNA e sul rapporto DNA:RNA:proteine di nuclei di fegato di ratto.