Phentolamine increases neuronal binding and retrograde transport of dopamine beta-hydroxylase antibodies.
When 125I-labelled antibodies against dopamine beta-hydroxylase (DBH) were injected into the anterior eye chamber of guinea-pigs they bound to sympathetic nerve terminals, were internalized into the axons and retrogradely transported to the ipsilateral superior cervical ganglion (SCG). This process was demonstrated to depend on specific binding sites since neutralized antibodies were not taken up and transported. The alpha-receptor antagonist phentolamine caused a 2.5-fold increase in binding in the iris and a 2.1-fold increase in accumulation of [125I]anti-DBH in the SCG. The results demonstrate that retrograde axonal transport of synaptic vesicle components is coupled to their turnover in nerve terminals.
D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D000906:Antibodies; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism; D002417:Cattle; D004299:Dopamine beta-Hydroxylase; D005728:Ganglia, Sympathetic / Q000502:physiology; D007498:Iris / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology*; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism
D000906:Antibodies; D011971:Receptors, Immunologic; D004299:Dopamine beta-Hydroxylase; D010646:Phentolamine