[In vitro maturation of reticulocytes. Behavior of RNA and inorganic pyrophosphatase].
A simple system of incubation of reticulocytes with a small degree of haemolysis has been developed. It was possible to test the influence of inorganic phosphate, pH-values in the range of 7.0--9.0 and anaerobic conditions on the in vitro maturation of reticulocytes. As main criteria of the maturation the RNA-content and the activity of PPase has been tested. The decrease of the RNA-content and the activity of the PPase is highly significant during maturation. The decrease of RNA and the reduction of reticulocytes are stimulated by inorganic phosphate.
D000693:Anaerobiosis; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000378:metabolism; D011755:Pyrophosphatases / Q000097:blood*; D012313:RNA / Q000097:blood*; D011817:Rabbits; D012156:Reticulocytes / Q000166:cytology* / Q000378:metabolism
D003470:Culture Media; D010710:Phosphates; D012313:RNA; D011755:Pyrophosphatases
In vitro-Reifung von Retikulozyten. Verhalten von RNS und anorganischer Pyrophosphatase.