Idiotypic analysis of anti-GL phi antibodies. I. Identification and strain distribution of GL-1 idiotypes.
We utilized both the inhibition of antigen binding and direct idiotype binding methods to identify a new set of common idiotype determinants on anti-GL antibodies of various mouse strains. Three anti-idiotypic antisera, each prepared against individually purified B10.WB anti-GL phi antibodies, were able to detect antibody-combining site-associated common idiotypic determinants, designated GL-1 idiotype(s), in antisera with GLT-binding activity obtained from all mouse strains except strains bearing Igh-1e allotype. Anti-GL phi antisera obtained from rabbits, guinea pigs, and rats did not express detectable levels of GL-1 idiotypes. Nonresponder mice to GL phi, upon immunization with GL-F gamma G or GL phi-F gamma G produced anti-GL antibodies expressing GL-1 idiotypes. Although the magnitude of the immune response to various GL-containing polymers is controlled by distinct Ir genes, the common GL-related antigenic determinants on these polymers are able to induce anti-GL antibodies with GL-1 idiotypic specificities.
D000818:Animals; D001666:Binding Sites, Antibody; D000939:Epitopes; D005971:Glutamates; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology; D007130:Immunoglobulin Idiotypes; D008239:Lysine; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D010649:Phenylalanine; D011108:Polymers; D011817:Rabbits; D051381:Rats
D000939:Epitopes; D005971:Glutamates; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D011108:Polymers; D010649:Phenylalanine; D008239:Lysine