Anti-receptor antibody-induced suppression of murine H-Y-specific delayed-type hypersensitivity responses.
A putative anti-H-Y receptor antiserum (ARA) was raised in C57BL/6 male mice against splenic T lymphocytes from syngeneic females immunized against H-Y antigen. When this antiserum is given i.v. to C57BL/6 females it prevents the expression of H-Y-specific delayed-type hypersensitivity (DTH). The suppressive activity in ARA was selectively retained on rabbit anti-mouse immunoglobulin columns, and could be absorbed by H-Y-immune spleen cells from C57BL/6 female mice. The abrogation of H-Y DTH reactivity was at least in part due to the generation of suppressor T cells which are generated by ARA in naive female mice. ARA-generated suppressor cells specifically inhibit the induction phase of DTH responses to the H-Y antigen, having no effect on (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific cutaneous sensitivity responses or on DTH responses to minor histocompatibility antigens. Furthermore, there is a requirement for Igh gene homology between the strain producing the ARA and the strain in which the DTH response is induced. Thus, C57BL/6 ARA given to A.BY (H-2b, Igh-1e) or to B.C-8 (H-2b, Igh-1a) mice was unable to suppress homologous H-Y DTH responses in these strains. However, C57BL/6 ARA induced suppressor cells in B.C-8 mice which were capable of inhibiting H-Y DTH responses when adoptively transferred to C57BL/6 females.
D000042:Absorption; D000818:Animals; D000906:Antibodies; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens / Q000235:genetics; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007136:Immunoglobulins; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011817:Rabbits; D011971:Receptors, Immunologic / Q000276:immunology*; D012730:Sex Chromosomes / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014998:Y Chromosome / Q000276:immunology*
D000906:Antibodies; D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera; D007136:Immunoglobulins; D011971:Receptors, Immunologic