Protein A-gold complex for postembedding staining of intracellular antigens.
The use of protein A-gold complex for electron microscopic demonstration of intracellular antigenic sites in thin sections of glutaraldehyde and Epon-embedded tissue is reported. The method is carried out in two steps: thin sections are incubated with specific antisera and the antigen-antibody complexes are then visualized with protein A-gold solution. So far several exocrine pancreatic enzymes as well as polypeptide hormones from the endocrine pancreas have been revealed by this technique.
D000681:Amylases / Q000378:metabolism; D000818:Animals; D003102:Colloids; D006046:Gold; D007118:Immunoassay / Q000379:methods*; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis; D008854:Microscopy, Electron; D009940:Organoids / Q000032:analysis; D010179:Pancreas / Q000032:analysis / Q000648:ultrastructure; D051381:Rats; D013205:Staphylococcal Protein A
D003102:Colloids; D007328:Insulin; D013205:Staphylococcal Protein A; D006046:Gold; D000681:Amylases