[Ultracryotomy: development and application (with examples from the yeast cytology) (author's transl)].
The preparation steps usually necessary for obtaining ultrathin frozen sections of biological material (chemical prefixation, enclosing, cryoprotective treatment, freezing, sectioning, and post-staining the sections for transmission electron microscopy) are submitted to a critical analysis. The application of cryo-ultramicrotomy, in particularly for cytochemical purposes, is reviewed. Fundamental considerations of chemical prefixation and poststaining are supported by examples from yeast cytology. Furthermore, the efficiency of the cryo-ultramicrotomy (electron optical resolution of ultrastructural details) is demonstrated on yeast cells and protoplasts.
D005404:Fixatives; D005629:Frozen Sections / Q000379:methods*; D008854:Microscopy, Electron; D008867:Microtomy / Q000379:methods*; D011523:Protoplasts / Q000648:ultrastructure; D013194:Staining and Labeling; D015003:Yeasts / Q000166:cytology / Q000648:ultrastructure
D005404:Fixatives
Gefrier-Ultramikrotomie: Entwicklungsstand und Anwendungsmöglichkeiten (mit Beispielen aus der Hefecytologie).