[Characteristics of the factors that suppress and stimulate interferon production].
A repressor of interferon production is contained in the hyaloplasm of the chick embryo fibroblast cells in the state of hyporeactivity. A factor stimulating interferon production (FSIP) was found in the hyaloplasm of the cells subjected to single or double induction with poly (pI) x poly (pC) followed by exposure to actinomycin D. Both preparations were of the protein nature and possessed high biological activity. They repressed or stimulatd interferon production at a dilution of 1:512-1:1024. The preparations had tissue and inductor specificity, did not affect the Venezuelan equine encephalomyelitis virus and interferon antiviral activity. Unlike the repressor, the FSIP proved to be thermolabile and pH-sensitive in acid media. The half lives of the repressor and stimulator were about 10 and 5 hours respectively.
D000327:Adsorption; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000037:antagonists & inhibitors*; D011070:Poly I-C / Q000494:pharmacology; D012097:Repressor Proteins / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014776:Virus Cultivation
D007369:Interferon Inducers; D012097:Repressor Proteins; D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide; D011070:Poly I-C
Kharakteristika faktorov, podavliaiushchikh i stimuliruiushchikh produktsiiu interferona.