Release of neuropeptide Y in response to splanchnic nerve stimulation in the conscious calf.
Plasma concentrations of neuropeptide Y were determined by means of a specific radioimmunoassay in the conscious calf. Significantly higher concentrations were found in the adrenal venous effluent (102 +/- 34 pmol/l) compared to systemic arterial plasma (11 +/- 7 pmol/l). Stimulation of the splanchnic nerves at 40 Hz in bursts for 1 s at 10 s intervals resulted in a slow sustained rise in plasma neuropeptide Y in both adrenal (peak 1124 +/- 217 pmol/l and peripheral (peak 771 +/- 182 pmol/l) samples. Experiments performed in adrenalectomized calves produced a closely similar release of neuropeptide Y when the nerves were stimulated intermittently in the same pattern and under the same conditions. Stimulation of the splanchnic nerves at 4 Hz continuously resulted in a smaller response. Pre-treatment with hexamethonium completely abolished the rise in plasma neuropeptide Y concentrations.
D000311:Adrenal Glands / Q000378:metabolism; D000315:Adrenalectomy; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000097:blood*; D009478:Neuropeptide Y; D011863:Radioimmunoassay; D013153:Splanchnic Nerves / Q000187:drug effects / Q000502:physiology*
D006584:Hexamethonium Compounds; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D018738:Hexamethonium