[Characteristics of the active and inactive variants of Actinomyces sp. 26-115, a producer of actinomycin C].
Two natural variants, i.e. No. 1 and No. 2, not producing actinomycin were isolated from cultures of the actinomycin C-producing organism Actinomyces sp. 26-115. Variant No. 1 differed from the active variant by the growth dynamics and colony morphology. Variant No. 2 was close to the active variant by the growth dynamics. It was shown with electron microscopy that the cells of variant No. 1 differed from those of the active variant in the number and form of the mycelial septa, more even and compact structure of the cell walls and higher sensitivity to actinomycin. Still, they were more stable to the effect of lysozyme and ultrasound. The cell walls of the inactive variant No. 1 gradually lost teichoic acid during development, while the loss of peptidoglycan was observed only on transfer to the stationary phase. The cell walls of the active variant lost teichoic acid and peptidoglycan at the same time on transfer to the stationary phase. Peptidoglycans of both variants contained diaminopimelic acid (the configuration of which was not determined) and glycine (1:1) as differentiating amino acids. The two adjacent tetrapeptides were joined with one glycine radical. The peptidoglycan peptide chains of both variants contained muramic, glutamic and diaminopimelic acids and alanine (1:1:1:2). The peptidoglycans of the inactive variant No. 1 contained in addition valine and isoleucine. However, it is hardly probable that they are contained by the peptidoglycan peptide chains.
D000190:Actinomyces / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002473:Cell Wall / Q000032:analysis / Q000187:drug effects; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000494:pharmacology; D014644:Genetic Variation; D008854:Microscopy, Electron; D011523:Protoplasts / Q000187:drug effects
C030013:cactinomycin; D003609:Dactinomycin
Nekotorye osobennosti aktivnogo i neaktivnogo variantov Actinomyces sp. 26-115, produtsenta aktinomitsina C.