Effects of L-triiodothyronine on tubulin content in developing male and female rat brain.
The tubulin content and biochemical components were determined in the cerebrum, cerebellum and hypothalamus from intact and T3-treated male and female rats during early life. T3-treatment between 0 and 9 days of age increased soluble protein, RNA DNA and tubulin content (mg per g tissue) in the 10-day-old male cerebellum but not in the cerebrum and hypothalamus except for soluble protein and tubulin (mg per g tissue), respectively. Intracellular tubulin content (mg per mg DNA) was increased by the T3-treatment in the 10-day-old male hypothalamus but not the other regions. When T3 was administered between 10 and 19 days, there was little effect of the treatment; increased tubulin (mg per g tissue) in the cerebrum and decreased RNA (mg per g tissue) and a ratio of tubulin to protein in the cerebellum from 20-day-old males. Less response to T3-treatment was observed in female cerebrum and hypothalamus but not in the cerebellum, compared with the male. These results suggest that the effect of T3-treatment on brain is modified by several factors such as tissue specificity, age-dependency and sexual differences. Modification by these factors might depend, at least in part, on changes in the number of T3-receptors due to the hormone treatment.
D000375:Aging; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D014284:Triiodothyronine / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D014404:Tubulin; D014284:Triiodothyronine; D012313:RNA; D004247:DNA