[Structure and function of DNA--membrane contact in cells].
A model of DNA-membrane contact based on the postulate about the determining role of membrane RNA (mbRNA) in providing its specifitity, stability and functional activity is proposed. Proceeding from the DNA-membrane contact structure, unity and interconditionality of reduplication and transcription processes are shown, and basic peculiarities of reduplication are discussed. It follows from the analysis of lysozyme-detergent treatment that two different DNA-membrane complex preparations can be obtained: one of these upon the spheroplast - "turned inside out spheroplast" transition, the other, containing specific DNA-membrane contact only - in the course of further detergent treatment. The parameters of the spheroplast - "turned inside out spheroplast" transition can be used to characterize the native DNA-membrane complex. It is concluded that RNA that stabilizes and makes the compact form of bacterial nucletide (f-DNA) is none other than the postulated mbRNA of the cellular DNA-membrane contact.
D001419:Bacteria / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D003902:Detergents; D012329:RNA, Bacterial; D003851:Deoxyribonucleases; D012260:Ribonucleases; D009113:Muramidase
O strukture i funktsii kontakta DNK--membrana v kletke.