Glycoproteins of axonal transport: affinity chromatography on fucose-specific lectins.
Rapidly transported fucose-labeled glycoproteins from axons of rabbit retinal ganglion cells were solubilized with nonionic detergents. The solubilized components were subjected to affinity chromatography on three different fucose-specific lectins. A recently characterized fucose-specific lectin from Aleuria aurantia bound reversibly approximately 60% of the applied protein-bound radioactivity. The lectins from Lotus tetragonolobus and Ulex europaeus bound are very small proportions of the labeled rapidly transported glycoproteins.
D000818:Animals; D001370:Axonal Transport; D002846:Chromatography, Affinity / Q000379:methods; D005643:Fucose; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D037102:Lectins; D011817:Rabbits; D013045:Species Specificity; D014316:Tritium
D006023:Glycoproteins; D037102:Lectins; D014316:Tritium; D005643:Fucose