Isolation of a cDNA clone containing a sequence complementary to the intestinal calcium-binding protein of the chick.
The present work describes the construction of a cDNA library in pBR322 plasmid from an mRNA population enriched for the intestinal calcium-binding protein (CaBP) mRNA of the chick. We report the isolation of one recombinant clone containing a vitamin D-regulated sequence, which is complementary to part of the CaBP mRNA. Northern blot hybridization experiments allowed us to identify a 1900 nucleotide RNA species as the CaBP mRNA.
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA