The isolation of cloned cDNA sequences which are differentially expressed in human lymphocytes and fibroblasts.
Poly(A)+ RNA populations derived from normal lymphocytes and fibroblasts have been compared by hybridising each RNA to cDNA derived from the other RNA population. This indicated that approximately 75% of the sequences were common to both, and that these were present at different concentrations in the two cell types. The two RNA populations were further compared by hybridising them to a cDNA recombinant library derived from lymphocyte poly(A)+ RNA. This allowed the identification of clones containing sequences which are abundant in lymphocyte poly(A)+ RNA but absent or rare in fibroblast poly(A)+ RNA. A direct estimation of the abundance of five of these sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.
D000818:Animals; D004247:DNA; D004274:DNA, Recombinant / Q000302:isolation & purification*; D005347:Fibroblasts / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000032:analysis*; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A; D012313:RNA; D012333:RNA, Messenger
D004274:DNA, Recombinant; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA