Antigen-induced bronchial anaphylaxis in actively sensitized guinea pigs. The effect of booster injection and cyclophosphamide treatment.
This investigation examines the effect of a booster dose and cyclophosphamide treatment in guinea pigs sensitized according to different sensitization regimens. The results show that guinea pigs sensitized with a high dose of ovalbumin (10 microgram) develop a transient bronchial reactivity. The administration of a booster dose to guinea pigs sensitized according to this regimen does not result in any secondary response. Cyclophosphamide 30 mg/kg given 2 days before the booster injection or the primary sensitization results in a marked increase in bronchial reactivity when challenged with ovalbumin. This increased bronchial reactivity is reflected in the IgE-like antibody titre examined by PCA. Guinea pigs primarily sensitized with a low dose of ovalbumin (0.5 microgram) show no bronchial reactivity when challenged with antigen. However, guinea pigs sensitized according to this regimen and given a booster dose show a marked secondary response when challenged 7 days after the booster injection. An examination of the antibody classes by PCA show that the booster injection causes an increase in the IgG1--and also in the IgE-like antibody production.
D000536:Aluminum Hydroxide / Q000276:immunology; D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001980:Bronchi / Q000276:immunology*; D003520:Cyclophosphamide / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006168:Guinea Pigs; D006636:Histamine Release; D007114:Immunization; D007117:Immunization, Secondary; D008170:Lung Compliance; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis
D000941:Antigens; D000536:Aluminum Hydroxide; D003520:Cyclophosphamide; D010047:Ovalbumin