Preferential stimulation by aurintricarboxylic acid of DNA-dependent RNA polymerase II activity in isolated larval nuclei of Artemia salina.
Aurintricarboxylic acid was found to stimulate preferentially RNA polymerase II activity in isolated nuclei of Artemia salina larvae. Studies on the in vitro transcription of a homologous chromatin indicate that this dye induces changes in the chromatin so as to enhance its template capacity for free RNA polymerase. Both free RNA polymerases I and II are equally sensitive to this dye in the absence of chromatin template. RNA polymerase II, however, becomes highly resistant to aurintricarboxylic acid when it is actively transcribing the chromatin template, whereas RNA polymerase I remains extremely sensitive even in the transcription complex. Therefore, in isolated nuclei, a preferential stimulation of alpha-amanitin-sensitive, but not-resistant RNA synthesis by aurintricarboxylic acid can be observed.
D000818:Animals; D001156:Artemia; D001312:Aurintricarboxylic Acid / Q000494:pharmacology*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007814:Larva; D012313:RNA / Q000096:biosynthesis; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D013698:Templates, Genetic
D002843:Chromatin; D003509:Cyclohexanecarboxylic Acids; D001312:Aurintricarboxylic Acid; D012313:RNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I