Development and application of a radioimmunoassay for pepleomycin.
A rapid sensitive radioimmunoassay for pepleomycin (PM) has been developed using an antiserum produced in a sheep in response to a bovine serum albumin-drug conjugate. Radioiodinated PM was prepared using Iodogen. The assay was reproducible with a limit of detection in plasma of 200 ng/liter. The antiserum does not cross-react with bleomycin or with a number of unrelated drugs. Pharmacokinetic studies using the assay were carried out in three patients who received a short iv infusion of 5 mg/m2 of PM. The drug disappeared from the plasma with an elimination half-life of approximately 3 hours.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000032:analysis* / Q000097:blood; D001761:Bleomycin / Q000032:analysis* / Q000097:blood; D006207:Half-Life; D007700:Kinetics; D017663:Peplomycin; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012756:Sheep
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D017663:Peplomycin