Demonstration of C-type viruses in N-methyl-N-nitroso urea (MNU)-induced leukemia of mice by reverse transcriptase activity and XC assay.
A significantly higher MuLV expression was demonstrated in cells of MNU-induced leukemia of mice compared to corresponding cells of untreated control animals by reverse transcriptase activity and XC cell assay. These positive findings were verified by determination of indirect immunofluorescence test to look for intracytoplasmic MuLV p30. The problem is discussed whether these viruses play a role in chemical leukemogenesis.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D001358:Avian Sarcoma Viruses; D009052:Leukemia Virus, Murine / Q000302:isolation & purification*; D007942:Leukemia, Experimental / Q000139:chemically induced / Q000382:microbiology*; D008770:Methylnitrosourea; D051379:Mice; D008828:Microbiological Techniques; D009607:Nitrosourea Compounds; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*; D012190:Retroviridae / Q000302:isolation & purification*
D000951:Antigens, Neoplasm; D009607:Nitrosourea Compounds; D008770:Methylnitrosourea; D012194:RNA-Directed DNA Polymerase