Cyclic AMP and immune responses: identification of prostaglandin E-producing cells in mouse spleens.
Previous work has shown that normal mice respond to the intravenous injection of sheep erythrocytes (SRBC) with a transient increase in splenic cyclic AMP peaking after 2 minutes, and this cyclic AMP response has been shown to be due to prostaglandins (PGs) released from antigen-specific cells. In the present study, the cell type responsible for PG production in response to antigen was determined using a fluorescence-activated cell sorter (FACS) and an electron microscope. C57Bl/6 mice injected with SRBC showed a 2% increase of PGE-producing spleen cells relative to controls. In control mice injected with phosphate-buffered saline (PBS), 19% of the spleen cells produced PGE. An electron microscopic study of PGE-producing cells sorted on FACS showed that they were small lymphocytes and plasma cells. In addition, nude mice also increased the number of PGE-producing spleen cells in response to antigen. These findings suggest that lymphocytes involving at least B-cells respond to antigen with an increase in PGE production.
D000818:Animals; D002452:Cell Count; D000242:Cyclic AMP / Q000378:metabolism*; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005453:Fluorescence; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010950:Plasma Cells / Q000276:immunology*; D011458:Prostaglandins E / Q000096:biosynthesis*; D013154:Spleen / Q000166:cytology*
D000939:Epitopes; D011458:Prostaglandins E; D000242:Cyclic AMP