title: [Binding of protein SCP to myelin, its identity with protein P2. A simple method of protein P2 isolation].
abstract: Protein SCP is found in myelin of spinal cord and spinal roots. It is shown that its amount accounts for 12% of the total protein content in myelin of spinal roots and only for 2% in myelin of spinal cord. Almost all the studied protein is extracted from myelin with 0.1 M NaCl (80-90%). The absolute identity of protens SCP and P2 is established using the cross reaction immunodiffusion with monospecific antisera. It is shown that- N-terminal amino acid in protein SCP, like in protein P2 is blocked. On the basis of the data obtained a conclusion is made that protein P2 is not an integral protein of myelin. However, myelin is capable under conditions of a nonionic medium of binding protein which then may be easily extracted by increasing the medium ionic strength. This gave reasons to propose a method for protein P2 isolation from myelin using 0.15 M NaCl with the subsequent purification by means of Sephadex G-50 gelfiltration.
mesh_terms: D000818:Animals; D002417:Cattle; D008297:Male; D004676:Myelin Basic Protein / Q000302:isolation & purification* / Q000378:metabolism; D018996:Myelin P2 Protein; D009186:Myelin Sheath / Q000378:metabolism; D011485:Protein Binding; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D018996:Myelin P2 Protein
vernacular_title: Sviaz' belka SCP s mielinom, identichnost' ego belky P2. Prostoĭ metod vydeleniia belka P2.