Two-site assay of human alpha 1-fetoprotein using 125I-labelled monoclonal antibodies.
Monoclonal antibodies to human alpha 1-fetoprotein (AFP) have been compared with a conventionally produced antiserum using radioimmunoassay and two-site immunoradiometric techniques. A low-affinity antibody, which proved inadequate for use in a radioimmunoassay, gave a satisfactory dose-response curve in a rapid two-site assay. A higher-affinity antibody yielded a simple, rapid, and sensitive two-site assay suitable for routine measurement of serum AFP.
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007457:Iodine Radioisotopes; D051379:Mice; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*; D012756:Sheep / Q000276:immunology; D000509:alpha-Fetoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D000509:alpha-Fetoproteins