[Preparation of subtilisin BPN' and dextran conjugates].
The conditions for subtilishine BPN' binding with bromocyanogen activated dextranes have been selected. The dependence of the enzyme activity and stability from pH and temperature levels has been studied. The stability of a modified enzyme during storage in solution is increased as compared with that of the native enzyme due to a decrease in the autolysis rate. On the basis of diffusion coefficients and sedimentation constants of conjugates, absolute values of their molecular weights have been computed.
D003488:Cyanogen Bromide; D003911:Dextrans; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D013381:Subtilisins / Q000378:metabolism*; D013696:Temperature
D003911:Dextrans; D004800:Enzymes, Immobilized; D013381:Subtilisins; D003488:Cyanogen Bromide
Poluchenie konbiugatov subtilizina VPN' s dekstranami.