title: Effect of bleomycin on mouse haemopoietic colony forming cells in culture (CFUc).
abstract: The kinetics of CFUc studied by hydroxyurea and endotoxin treatments indicate that this cell population is proliferating actively (some 50% in S-phase). This fact implies a peculiar response to (BLM) treatment with Bleomycin, a drug which appears to be proliferation-dependent. The dose-response has a steep initial slope resulting in a low extrapolation number (n = 0.66; D37 = 960 +/- 70 mg BLM/kg body weight). The steep initial slope is confirmed by split-dose experiments resulting in a potentiation effect by fractionation. Further evidence for the dependence of the action of this drug upon the proliferative state of the cell population is derived from time-response studies after single doses of BLM.
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D051379:Mice
chemical_list: D004731:Endotoxins; D001761:Bleomycin; D006918:Hydroxyurea