Immunochemical distinction between two different chains of type IV collagen.
Antisera against mouse and human basement-membrane type IV collagen showed in radioimmunoassays distinct binding with large pepsin fragments obtained from the C-terminal portions of alpha 1 (IV)- and alpha 2 (IV)-chains. These reactions were specific for each constituent polypeptide chain. The data were confirmed by immunoadsorption, allowing the separation of antibodies with restricted chain specificity. Inhibition assays with CNBr peptides demonstrated the different localizations of antigenic determinants, which were either species-specific or shared by the human and mouse antigens.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001485:Basement Membrane / Q000032:analysis; D003094:Collagen / Q000276:immunology*; D003488:Cyanogen Bromide; D000939:Epitopes / Q000032:analysis; D006801:Humans; D051379:Mice; D010434:Pepsin A; D010446:Peptide Fragments / Q000276:immunology; D011863:Radioimmunoassay
D000939:Epitopes; D010446:Peptide Fragments; D003094:Collagen; D010434:Pepsin A; D003488:Cyanogen Bromide