[Peculiarities of nuclear genome transcription at early stages of liver regeneration. Possible role of mitochondrial translation in activation of nuclear RNA biosynthesis].
During the first hour following partial hepatectomy the mitochondria came in proximity with the nucleus and were concentrated around the electron-dense zones of euchromatin shifted towards the peripheral parts of the nucleus. After 1.5 hrs following the surgery the specific radioactivity of nuclear RNA was increased due to activation of nucleolar RNA biosynthesis. The activation of endogenous template activity of isolated nuclei by cytosol obtained 1 hr after the surgery was due to mobilization of "free", i. e. non-bound to the template, form of nuclear RNA polymerase. A preliminary administration of chloramphenicol completely blocked the mitochondrial tropism towards the nucleus and the activation of nuclear RNA biosynthesis. It was assumed that the activation of nuclear genome transcription at early stages of liver regeneration is mediated by mitochondrial translation. A possible mechanism of coordination of nuclear and mitochondrial genome activity based on identicity of mitochondrial RNA-polymerase and one of the subunits of nuclear RNA-polymerase I is discussed.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D002843:Chromatin / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005796:Genes; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D012313:RNA / Q000235:genetics*; D051381:Rats; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
D002843:Chromatin; D012313:RNA; D002701:Chloramphenicol; D012321:DNA-Directed RNA Polymerases
Osobennosti transkriptsii iadernogo genoma na rannikh étapakh regeneratsii pecheni. Vozmozhnaia rol' mitokhondrial'noĭ transliatsii v aktivatsii biosinteza iadernoĭ RNK.