[Several approaches to purifying rat liver G2-chalone].
An attempt was made to isolate the liver tissue chalone blocking the transition of hepatocytes from G2 period of the cell cycle into mitosis/g2-chalone/. The purification was carried out using gel filtration through Sephadex G-75 and hydroxyapatite chromatography as well as by means of ultrafiltration through Diaflo membranes XM-300, XM-100 and PM-10. The biologically active preparation, obtained by these two procedures, was purified 100-fold. Molecular mass of G2 chalone was apparently less then 10,000; it penetrated through PM-10 membrane. The chalone appears to form complexes with high molecular carriers.
D000818:Animals; D050150:Chalones; D002850:Chromatography, Gel / Q000379:methods; D003911:Dextrans; D006131:Growth Inhibitors / Q000302:isolation & purification*; D006882:Hydroxyapatites; D008099:Liver / Q000032:analysis*; D008297:Male; D008722:Methods; D009930:Organic Chemicals; D051381:Rats; D014462:Ultrafiltration
D050150:Chalones; D003911:Dextrans; C025912:G(2) chalone; D006131:Growth Inhibitors; D006882:Hydroxyapatites; D009930:Organic Chemicals; C025614:sephadex
Nekotorye podkhody k ochistke G2-khalona pecheni krys.