The participation of trimethylammonium in the mouse erythrocyte epitope recognized by monoclonal autoantibodies.
Monoclonal IgM anti-erythrocyte autoantibody produced by a NZB-derived hybridoma has been found to react with trimethylammonium-containing compounds. Such compounds are able to prevent the lysis of bromelain-treated mouse erythrocytes (BrMRBC) by those autoantibodies. Using a column of insolubilized betaine hydrazine (BH) the monoclonal anti-erythrocyte antibody has been specifically retained. Elution of this antibody was accomplished by 0.15 M choline (Ch).
D000818:Animals; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001622:Betaine / Q000031:analogs & derivatives* / Q000276:immunology; D001963:Bromelains; D003429:Cross Reactions; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010767:Phosphorylcholine / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D000939:Epitopes; D000644:Quaternary Ammonium Compounds; D010767:Phosphorylcholine; D001622:Betaine; D001963:Bromelains; C012332:Girard's reagent T