Calcium binding sites of synaptosomes from insect nervous system as probed by trivalent terbium ions.
Terbium binding to synaptosomes from the central nervous system of the locust was studied by fluorescence spectroscopy and electron microscopy. The protein-sensitized fluorescence of terbium was used to characterize the calcium binding sites of synaptosomes. As judged by electron microscopy and x-ray analysis, terbium ions produced electron-dense patches in regular arrays on the outer surface of synaptosomal membranes and induced marked aggregation of synaptic vesicles in isolated terminals.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D005724:Ganglia / Q000378:metabolism; D006110:Grasshoppers; D007700:Kinetics; D008854:Microscopy, Electron; D012430:Ruthenium Red / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D013725:Terbium / Q000378:metabolism*
D013725:Terbium; D012430:Ruthenium Red; D002118:Calcium