Role of calcium and cAMP in the regulation of rat submandibular mucin secretion.
The role of cellular adenosine 3',5'-cyclic monophosphate (cAMP) and calcium in the secretion of [14C]glucosamine-labeled mucins by rat submandibular acinar cells was studied. cAMP appeared to be involved, since 1-methyl-3-isobutylxanthine potentiated the secretory response and cellular cAMP levels increased dramatically following adrenergic stimulation. Furthermore, cAMP analogues were able to elicit a secretory response in the absence of beta-adrenergic receptor activation. Cellular calcium was required for mucin secretion at the level of cAMP action; depletion of cellular calcium by pretreatment with EGTA inhibited the secretory response to both adrenergic stimulation and exogenous cAMP addition, but pretreatment with EGTA did not alter the rise in cellular cAMP induced by norepinephrine. Extracellular calcium was not required to elicit mucin secretion, nor could secretion be elicited by means of the calcium ionophore A23187 alone. However, extracellular calcium may have an important biological role in mucin secretion, since cholinergic receptor activation and alpha-adrenergic receptor activation in conjunction with beta-adrenergic receptor activation potentiated mucin release. In addition, the calcium ionophore A23187 potentiated mucin release following cAMP analogue addition.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*
D009077:Mucins; D004533:Egtazic Acid; D000242:Cyclic AMP; D007545:Isoproterenol; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine; D009638:Norepinephrine