The properties of two sea-squirt antigens.
The accuracy of radioimmunoassay (RIA) has been much improved for sea-squirt antigen Gi-x, which is considered to be of higher molecular weight than antigen Ei-M, by using radiolabeled Gi-x as a tracer. Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight of Gi-x type antigens, in contrast to Ei-M. However, since a considerable portion of the anti-genic activity gave a single peak in gel chromatography with Sepharose 6B, the substance in the peak fractions was isolated as a fairly homogeneous preparation and referred to as Gi-rep. Gi-rep showed distinct characteristics of Gi-x type antigens and was clearly discriminated from Ei-M by radioimmunometry in vitro. The in vitro observation also suggested that Gi-rep and Ei-M carried a common antigenic determinant (type alpha), but that Ei-M also carried a specific determinant (type beta). The weight-average molecular weights were 1.1 x 10(5) for Gi-rep and 2.3 x 10(4) for Ei-M. Both preparations consisted of acidic glycoproteins with considerable amounts of sulfate and phosphate.
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D001826:Body Fluids / Q000276:immunology; D002850:Chromatography, Gel; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis; D008970:Molecular Weight; D011863:Radioimmunoassay; D014561:Urochordata / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D006023:Glycoproteins