title: Aldehyde oxidase cross-reacting material in the Aldoxn, cin, mal, and lxd mutants of Drosophila melanogaster.
abstract: Rocker immunoelectrophoresis was used to estimate aldehyde oxidase cross-reacting material (AO-CRM) in larval hemolymph and adult fly extracts in mutants with reduced AO enzymatic activity. Hemolymph of larvae homozygous for Aldoxn, which is a mutation of the presumed structural gene for AO, contains 30% of the wild-type CRM. The demonstration of AO-CRM in Aldoxn larval hemolymph is surprising since this genotype has been reported to lack CRM. By contrast, adult Aldoxn flies lack detectable CRM. The other AO-deficient mutants that were examined are cin, mal, and lxd; each has appreciable levels of CRM in both larval hemolymph and adult extract. Detection of CRM in these mutants helps to clarify conflicting reports in the literature.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000276:immunology; D000818:Animals; D003429:Cross Reactions; D004331:Drosophila melanogaster / Q000235:genetics*; D000939:Epitopes; D006458:Hemolymph / Q000201:enzymology; D007122:Immunoelectrophoresis; D009154:Mutation; D010641:Phenotype
chemical_list: D000939:Epitopes; D000445:Aldehyde Oxidoreductases