[Participation of various adenosine and guanosine derivatives in the abortive RNA synthesis initiation reaction: effect of Mg2+, Mn2+, and rifampicin].
Adenosine, TMP, ADP, ATP and UpA along with guanosine and tis analogous derivatives have different reactivity towards [alpha-32P]UTP in abortive initiation reactions catalyzed by E. coli RNA polymerase on T2 DNA in the presence of Mg2+ or Mn2+. Rifampicin moderately inhibited almost all of the above mentioned reactions, except the ATP and the GTP which were even 2.5 times more reactive in the presence of this antibiotic.
D000227:Adenine Nucleotides / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D006150:Guanine Nucleotides / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D008345:Manganese / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D012318:RNA Polymerase I / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014544:Uridine Triphosphate / Q000378:metabolism
D000227:Adenine Nucleotides; D004279:DNA, Viral; D006150:Guanine Nucleotides; D008345:Manganese; D012313:RNA; D012318:RNA Polymerase I; D008274:Magnesium; D014544:Uridine Triphosphate; D012293:Rifampin
Uchastie razlichnykh proizvodnykh adenozina i guanozina v reaktsii abortivnoĭ initsiatsii sinteza RNK: vliianie ionov Mg2+, Mn2+ i rifampitsina.