Role of carbohydrate as an antigenic determinant of a glycoprotein from rye-grass (Lolium perenne) pollen.
The carbohydrate component of Glycoprotein 2 (12% carbohydrate) from rye-grass (Lolium perenne) pollen has saccharide sequences that contribute to its antigenicity. Radioimmunoassay inhibition tests show that the antiserum to this glycoprotein cross-reacts with a number of other plant glycoproteins. In contrast, antiserum to another glycoprotein from rye-grass pollen, Glycoprotein 1 (5% carbohydrate), does not cross-react with any of the test glycoconjugates. Treatment of glycoproteins with sodium metaperiodate (0.02 M, 4 degree C, 6 h, in the dark) causes the loss of their ability to cross-react antigenically with Glycoprotein 2, and a loss of capacity to bind 125I-labelled concanavalin A. The cross-reactivity of this plant glycoprotein with other glycoconjugates imposes limitations on the interpretation of ultrastructural studies aimed at localizing a particular glycoprotein to a cellular site by using fluorescent or ferritin-labelled antisera. A radioimmunoassay inhibition technique for quantitative determination of the amounts of antigens in plants is also described.
D002241:Carbohydrates / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D010504:Periodic Acid / Q000494:pharmacology; D010944:Plants / Q000032:analysis; D011058:Pollen / Q000276:immunology*; D011863:Radioimmunoassay
D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D010504:Periodic Acid; C009288:metaperiodate