Differential stain for mycobacteria that does not require heat.
A stain of 0.1% toluidine blue in a 1.0% aqueous solution of triethylene glycol followed by decolorization with acid-alcohol resulted in mycobacteria retaining the stain (violet), whereas non-acid-fast bacteria were decolorized.
D001431:Bacteriological Techniques; D009161:Mycobacterium / Q000166:cytology*; D011092:Polyethylene Glycols; D013194:Staining and Labeling / Q000379:methods*; D014048:Tolonium Chloride
D014048:Tolonium Chloride; C028914:triethylene glycol; D011092:Polyethylene Glycols