Immunoreactive pro-enkephalin and prodynorphin products are differentially distributed within the nucleus of the solitary tract of the rat.
In this study we examined the distribution of two different endogenous opioid peptides in the nucleus of the solitary tract of the rat medulla. As a marker for immunoreactive enkephalin, we used an antiserum directed against one of the proenkephalin products, methionine enkephalin-arg-gly-leu (m-Enk). To identify immunoreactive dynorphin we used an antiserum directed against the prodynorphin product, dynorphin B (Dyn B). The PAP method was used on both colchicine and normal animals. Caudal to the obex, within the commissural nucleus, there is extensive overlap of both immunoreactive m-Enk and Dyn B terminals and cells. While the cells are morphologically similar, the immunoreactive dynorphin cells are somewhat larger. Rostral to the obex, there is a marked difference in the distribution of the two compounds. Immunoreactive m-Enk terminals are concentrated medial to the solitary tract; there is minimal staining laterally. In contrast, immunoreactive Dyn B terminals are concentrated lateral to the solitary tract. The rostral cellular distribution of the two opioid peptides follows a similar pattern. The morphology of the medially located m-Enk and laterally located Dyn B cells is also readily distinguished. The former are small, round cells with minimal dendritic labelling; the latter are larger, pyramidal neurons with prominent apical and basal dendrites. Since the medial and lateral nuclei of the solitary tract have been associated with cardiovascular and respiratory control, respectively, these data suggest that different endorphin families have different functional actions within the nucleus of the solitary tract.
D000818:Animals; D002319:Cardiovascular System / Q000294:innervation; D002415:Cats; D002628:Chemoreceptor Cells / Q000378:metabolism; D004399:Dynorphins / Q000031:analogs & derivatives* / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D008526:Medulla Oblongata / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism; D011311:Pressoreceptors / Q000378:metabolism; D051381:Rats; D012137:Respiratory System / Q000294:innervation; D013045:Species Specificity; D014630:Vagus Nerve / Q000378:metabolism
D004723:Endorphins; D004744:Enkephalin, Methionine; D004399:Dynorphins; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-; C036200:rimorphin