[Isolation of a cellular hybrid secreting an antibody specific for human leukocyte interferon].
A cellular hybrid secreting a Mouse monoclonal immunoglobulin (IgG) specific of human leukocyte interferon has been obtained, following fusion of a nonsecreting variant of the myeloma P3X63 Ag8 with splenic lymphocytes from a Mouse immunized against interferon incorporated into liposomes. The antibody also recognizes human interferon made by a bacterial recombinant and a fraction of interferon made by Namalva cells. Mass production of the antibody has been achieved using ascitic growth of the hybrid in Mice.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007372:Interferons / Q000096:biosynthesis / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology; D008081:Liposomes; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D010954:Plasmacytoma; D013154:Spleen / Q000276:immunology
D007074:Immunoglobulin G; D008081:Liposomes; D007372:Interferons
Isolement d'un hybride cellulaire produisant un anticorps spécifique de l'interféron leucocytaire humain.