A new technique for the visualization of the cytoskeleton in cultured fibroblasts with Coomassie blue R250.
Staining of fibroblast monolayers with Coomassie brilliant blue R250 provides a relatively easy and fast method for visualization of cytoskeletal elements. Staining of detergent-extracted monolayers improves resolution and provides preparations comparable in definition to those obtained by specific immunofluorescence.
D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D008870:Microtubules / Q000648:ultrastructure*; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R