Synthesis of biologically active interleukin 2 by Xenopus oocytes in response to poly(A)-RNA from a gibbon T-cell line.
The poly(A)-containing RNA isolated from a gibbon lymphosarcoma cell line, MLA144, known to release Interleukin 2 (IL2) was microinjected into Xenopus oocytes. The incubation medium from injected oocytes stimulated the DNA synthesis of an IL2-dependent cell culture and also maintained its viability. DNA-stimulating activity appeared in the oocyte incubation medium 6 h after injection and continued to accumulate for at least 96 h. Thymidine incorporation by the IL2-dependent cells is proportional to the concentration of oocyte incubation medium added and the IL2 activity produced by the oocytes is proportional to the amount of poly(A)-RNA injected. Incubation medium of oocytes injected with RNA from another T-cell line, 6G1, which does not produce IL2, did not contain DNA-stimulating activity. The same assay also showed that the IL2 mRNA sedimented at 14-16 S in a nondenaturing sucrose gradient. A series of monoclonal antibodies prepared against human IL2 neutralized the DNA-stimulating activity released by the injected oocytes to the same extent as they neutralized human IL2.
D000818:Animals; D000911:Antibodies, Monoclonal; D002143:Callitrichinae; D002460:Cell Line; D004261:DNA Replication; D005260:Female; D006825:Hybridomas / Q000276:immunology; D006922:Hylobates; D007376:Interleukin-2 / Q000235:genetics*; D007700:Kinetics; D008222:Lymphokines / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D013601:T-Lymphocytes; D014981:Xenopus
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D008222:Lymphokines; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA