Mobile dispersed genetic element MDG1 of Drosophila melanogaster: transcription pattern.
Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-length transcript represented by approximately 29S poly(A)+RNA. Besides it, approximately 15S poly(A)+RNA is present in the cytoplasm of culture cells which corresponds to the 3'-part of 29S RNA and possibly to a small region near the 5'-end of the latter. The major fraction of mdg1 transcripts is present in the fraction of free cytoplasmic RNP particles while only 10-20% are recovered in polysomes. In this respect, mdg1 is similar to mdg3 and quite different from two single-copy genes transcripts of which are almost completely recovered in polysomes. The relationship between mobile dispersed genetic elements and endogeneous viral genomes is discussed.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000378:metabolism*; D005796:Genes; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000096:biosynthesis; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes