[Frequency of chromosome aberrations induced in human peripheral lymphocytes by in vitro 60Co gamma quanta at doses of 1 to 5 Gr. in an analysis of the 1st-division cells at different fixation times].
The quantitative analysis of chromosomal aberrations in the first division cells of 50-, 54-, 58-, 52- and 66-hour peripheral blood lymphocytes cultures of healthy donors was performed after irradiation in vitro with 60Co gamma-quantums at doses 1--5 Gy. Cells of the first division were identified by a differential staining of sister chromatid method using 5-bromdeoxyuridine. No significant differences in frequencies of aberrant cells and aberrations of chromosomal type were found between cultures fixed at different times. The distribution of dicentrics in cells did not differ from the Poisson distribution regardless of fixation times and doses. On the basis of these findings it is concluded that chromosomes of human peripheral blood lymphocytes passing the cell cycle at different rates have approximately equal radiosensitivity.
D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes / Q000009:adverse effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects*; D008938:Mitosis / Q000528:radiation effects*; D011336:Probability; D012854:Sister Chromatid Exchange / Q000528:radiation effects; D013194:Staining and Labeling; D013997:Time Factors
D003037:Cobalt Radioisotopes
Chastota khromosomnykh aberratsiĭ, indutsirovannykh v limfotsitakh perifericheskoĭ krovi cheloveka in vitro gamma-kvantami 60Co v dozakh 1--5 Gr, pri analize kletok pervogo deleniia v raznye sroki fiksatsii.