[Nomarski interference microscopy: use of combined contrast uranyl acetate-PTA for in vitro observation of monolayer cultures].
In this paper is reported a technique for the fixation and staining of cellular monolayers in vitro for observation by the interferential microscope according to Nomarski. This method includes the use of glutaraldehyde at 2.5% in phosphate buffer 0.1 M pH 7.5 and the sequential use of Uranil acetate (0.9% in absolute alcohol) and phosphtungstic acid (1% in absolute alcohol). This study has been conducted on 3 cellular lines: PC12 (rat pheocromocytoma), R.P.C. (rat pineal cells), primary culture obtained from a human carcinoma of the uterus.
D000085:Acetates; D000818:Animals; D002277:Carcinoma; D002478:Cells, Cultured; D005260:Female; D005404:Fixatives; D006801:Humans; D008857:Microscopy, Interference / Q000379:methods*; D010673:Pheochromocytoma; D010772:Phosphotungstic Acid; D010870:Pineal Gland; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D014501:Uranium; D014594:Uterine Neoplasms
D000085:Acetates; D005404:Fixatives; D010772:Phosphotungstic Acid; D014501:Uranium
Microscopia interferenziale secondo nomarski: uso del contrasto combinato acetato di uranile-PTA nell'osservazione di colture in vitro a monostrato.