Protein synthesis in rabbit reticulocytes. Co-eIF-2A reverses mRNA inhibition of ternary complex (Met-tRNAf.eIF-2.GTP) formation by eIF-2.
mRNAs, at low concentrations, drastically inhibit ternary complex formation by eIF-2 (Met-tRNAf.eIF-2.GTP) and, when added to the preformed ternary complex, cause extensive dissociation of the complex. Co-eIF-2A stimulates (2- to 4-fold) Met-tRNAf binding to eIF-2 and, in the presence of excess Co-eIF-2A, the stimulated Met-tRNAf binding to eIF-2 is fully resistant to mRNAs. Other cofactors tested such as Co-eIF-2B and Co-eIF-2C do not reverse mRNA inhibition of ternary complex formation.
D000818:Animals; D001798:Blood Proteins; D015852:Eukaryotic Initiation Factor-2; D005914:Globins / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D006160:Guanosine Triphosphate / Q000097:blood*; D007700:Kinetics; D010448:Peptide Initiation Factors / Q000097:blood* / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000097:blood*; D012358:RNA, Transfer, Met; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*
D001798:Blood Proteins; D015852:Eukaryotic Initiation Factor-2; D020662:Guanine Nucleotide Exchange Factors; D010448:Peptide Initiation Factors; D011506:Proteins; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D006160:Guanosine Triphosphate; D005914:Globins