Biologic properties of chromatographically separated murine thymoma-derived Interleukin 2 and colony-stimulating factor.
Two growth factors, interleukin 2 (T cell growth factor) and colony-stimulating factor, are produced concomitantly by a murine EL-4 thymoma cell line after stimulation by phorbol myristate acetate. As shown elsewhere, these thymoma-derived factors appear to be biochemically and functionally indistinguishable from the interleukin 2 and colony-stimulating factor produced by mitogen-stimulated mouse spleen cells. Both factors co-elute during gel filtration with apparent m.w. in the range of 30,000, and both exhibit overlapping isoelectric point profiles between pH 4 and pH 5. Because we were unable to separate these 2 factors by methods based on either m.w. or charge, we have used phenyl-Sepharose chromatography, a method based on hydrophobic interactions, to completely separate murine interleukin 2 and colony-stimulating factor. In contrast with published reports, each of the separated factors exhibits unique biologic activities on lymphocytes and macrophages. Interleukin 2 provides help for antibody synthesis in the nude mouse, but neither enhances interferon production by macrophages nor stimulates macrophage growth. Colony-stimulating factor does not enhance antibody synthesis in the nude mouse but does enhance interferon production by macrophages and stimulate macrophage growth.
D000818:Animals; D000917:Antibody Formation; D002845:Chromatography; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008970:Molecular Weight; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013945:Thymoma / Q000276:immunology*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D008222:Lymphokines; D003208:Concanavalin A; D007372:Interferons; D013755:Tetradecanoylphorbol Acetate