Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis.
Thirty-two Escherichia coli strains from 30 children with pyelonephritis were examined for their haemagglutination patterns and O and K serotypes. 29 (91%) of the strains showed mannose-resistant haemagglutination (MRHA). By use of well-defined target cells, these MRHA+ strains could be shown to recognise human cells either in a P-specific manner (recognising a specific galactosyl-galactose structure which is part of P blood groups antigens) or in a separate, X-specific manner. Both recognition mechanisms could occur separately or together on the same bacteria, the frequencies of P and X specificity being 81 and 19%, respectively. Both MRHA and P specificity were significantly associated with the O antigens 01, 04, 06, 016, and 018, and the capsular antigen K1, which have previously been associated with pyelonephritis. However, the association of MRHA and P specificity with upper urinary tract infection in children is greater than that of any other laboratory-defined bacterial characteristic.
D000942:Antigens, Bacterial / Q000276:immunology; D001789:Blood Group Antigens; D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000382:microbiology; D000939:Epitopes; D004926:Escherichia coli / Q000145:classification / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D007223:Infant; D008297:Male; D008358:Mannose / Q000494:pharmacology*; D010127:P Blood-Group System; D011704:Pyelonephritis / Q000209:etiology* / Q000276:immunology / Q000382:microbiology; D012703:Serotyping
D000942:Antigens, Bacterial; D001789:Blood Group Antigens; D000939:Epitopes; D010127:P Blood-Group System; D008358:Mannose