Lack of effective messenger RNA for beta 2-microglobulin in a gestational human choriocarcinoma cell line (GCH-1).
When two lines of gestational human choriocarcinoma cells (GCH-1 and GCH-2) were incubated with [35S]methionine, the labeled beta 2-microglobulin was not detected on sodium dodecyl sulfate:polyacrylamide gel electrophoresis after purification of the labeled protein with affinity chromatography, using rabbit antibody against beta 2-microglobulin as the ligand. In contrast, in the case of nongestational choriocarcinoma cells originating from male stomach cells, the incorporation of [35S]methionine into beta 2-microglobulin was observed. When polyadenylate-containing messenger RNA prepared from GCH-1 cells was incubated with [35S]methionine in a rabbit reticulocyte lysate system, the labeling of beta 2-microglobulin was not shown. On the other hand, when polyadenylate-containing messenger RNA from nongestational choriocarcinoma cells was incubated in the same cell-free system, labeled beta 2-microglobulin was detected. These results indicate that beta 2-microglobulin is not synthesized in gestational human choriocarcinoma cells and at least in the case of GCH-1, beta 2-microglobulin is not synthesized owing to the lack of effective messenger RNA for beta 2-microglobulin.
D000818:Animals; D001609:Beta-Globulins / Q000096:biosynthesis*; D002460:Cell Line; D002822:Choriocarcinoma / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006680:HLA Antigens; D006801:Humans; D008297:Male; D008545:Melanoma; D008715:Methionine / Q000494:pharmacology; D008970:Molecular Weight; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes; D013270:Stomach; D001613:beta 2-Microglobulin / Q000032:analysis / Q000096:biosynthesis*
D001609:Beta-Globulins; D006680:HLA Antigens; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D008715:Methionine