Immunoferritin localisation of antigens of the microvillous membrane of human trophoblast.
Antiserum raised to purified plasma membranes of the human trophoblast (TrPM) was used in an indirect immunoferritin method to investigate the expression of specific membrane antigens in explants of human trophoblast and in cultured cells. Ferritin deposits occurred in discrete regions on the microvillous membrane of the syncytiotrophoblast, and these deposits also occurred in coated areas of the membrane. Discrete staining was also seen in cultured trophoblast cells and HeLa cells. Human amnion cells had a strong positive reaction for the TrPM antigens, but foetal skin cells and HEp-2 (larynx carcinoma) cells were negative. TrPM antigens appear to be highly specific for trophoblast and amnion, only being found otherwise as a result of placental genomic re-expression in neoplasia.
D000954:Antigens, Surface; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes; D005293:Ferritins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007328:Insulin / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology*
D000954:Antigens, Surface; D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007328:Insulin; D014168:Transferrin; D005293:Ferritins