Chromatography of human leukocyte interferon on controlled pore glass.
Human leukocyte interferon (HuIFN-alpha ) was adsorbed on controlled pore glass (CPG) beads in 0.01 M potassium phosphate, pH 7.2. Partial recovery fo HuIFN-alpha from CPG, ranging from 50% to 75%, was obtained with ethylene glycol (6.8 M), ammonium chloride (1.0 M) and Tris.HCl (1.0 M). A near complete recovery (90%) was obtained with potassium phosphate (1 M), but there was no selectivity in the elution vis á vis other proteins. An efficient elution of HuIFN-alpha was accomplished with tetramethyl-, and tetraethylammonium chlorides: the recovery of HuIFN-alpha was complete at low concentrations (0.25 M to 0.50 M) of these salts at pH 8.0 and the elution of interferon was more selective than with other eluants.
D000643:Ammonium Chloride; D005026:Ethylene Glycols; D005898:Glass; D006801:Humans; D007372:Interferons / Q000302:isolation & purification*; D007962:Leukocytes / Q000032:analysis*; D000644:Quaternary Ammonium Compounds; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds
D005026:Ethylene Glycols; D000644:Quaternary Ammonium Compounds; D013757:Tetraethylammonium Compounds; D000643:Ammonium Chloride; D019789:Tetraethylammonium; D007372:Interferons; C027917:tetramethylammonium