Purification and characterization of ovine astrovirus.
Astrovirus, purified in aggregated form from epithelial cells of the small intestinal villi of infected gnotobiotic lambs, was shown to have a single-stranded RNA genome with an S value of 34 and poly(A) tract. Only two major capsid polypeptides were detected with similar mol. wt. of approx. 3.3 x 10(4).
D000818:Animals; D002213:Capsid / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D007413:Intestinal Mucosa / Q000382:microbiology; D001257:Mamastrovirus / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D012367:RNA, Viral / Q000032:analysis*; D012756:Sheep / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis*; D014713:Viruses, Unclassified / Q000302:isolation & purification*
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D011061:Poly A; D012313:RNA