Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue.
In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained.
D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001842:Bone and Bones / Q000098:blood supply*; D002196:Capillaries / Q000201:enzymology*; D006651:Histocytochemistry; D009132:Muscles / Q000098:blood supply*; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling; D000516:alpha-Amylases / Q000378:metabolism*
D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D000681:Amylases; D000516:alpha-Amylases; D000251:Adenosine Triphosphatases