title: Increased nitroblue tetrazolium dye reduction by human neutrophils stimulated by interferon in vitro.
abstract: Human leukocyte interferon enhanced nitroblue tetrazolium dye (NBT) reduction by human neutrophils (PMNs). Increase in NBT reduction paralleled increase in interferon dose. When human leukocyte interferon was heated to 60 C or 80 C for 30 min, both the antiviral activity and the effect on NBT reduction decreased. Human leukocyte interferon neutralized with anti-human leukocyte interferon serum showed no effect on NBT reduction. A human fibroblast interferon preparation also enhanced NBT reduction. The species dependency of interferon was shown in NBT reduction as well as in antiviral activity.
mesh_terms: D000818:Animals; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology*; D007962:Leukocytes; D051379:Mice; D009504:Neutrophils / Q000187:drug effects*; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D011817:Rabbits; D013045:Species Specificity; D013696:Temperature; D013778:Tetrazolium Salts
chemical_list: D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D007372:Interferons