Practical aspects of diagnostic electron microscopy.
The electron microscope technique of negative staining was first used to obtain fundamental information about virus morphology, but in recent years it has developed into a practical method for virus diagnosis. The methods employed are both simple and rapid. The following review discusses in detail the steps that must be taken to obtain good electron microscope preparations and illustrates some of the results that can be obtained.
D006801:Humans; D007158:Immunologic Techniques; D008854:Microscopy, Electron / Q000379:methods*; D013048:Specimen Handling / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000276:immunology