Quantitative studies on immunofluorescent staining. VII. Fractionation of conjugates by acid dialysis to reduce nonspecific staining.
Dialysis of fluorescein-conjugated antibodies at pH 5.8-6.0 can reduce unwanted nonspecific staining. Selective precipitation of highly fluoresceinated proteins in mildly acidic buffer is a simple technique which can produce a reagent with greatly improved nonspecific staining characteristics with little or no loss of antibody activity. This technique may be especially useful when fluorescent antibody tests are to be performed on tissues which have a high propensity for nonspecific staining such as skin.
D003956:Dialysis; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005590:Fractional Precipitation; D006863:Hydrogen-Ion Concentration; D013194:Staining and Labeling
D005452:Fluoresceins