Antigen specific receptors on murine B cell lineage. 1. On antibody forming cells in various stages of immune response.
In this study it was examined whether or not antigen specific receptors are on direct and indirect plaque-forming cells (PFC). Spleen cells from mice primed both with horse red blood cells (HRBC) and sheep red blood cells (SRBC) were incubated with HRBC to make rosettes and fractionated on Ficoll-Hypaque density gradient. Both direct and indirect HRBC-PFC were depleted from the interface fraction. The depletion was antigen specific and inhibited by the preincubation of spleen cells with antiserum against kappa- or mu-chain for direct PFC and with antiserum against kappa- or gamma 1-chain for indirect PFC. The depletion was not due to the rosette formation mediated by Fc-receptors which might be on antibody forming cells, because PFC were not eliminated by the density gradient sedimentation after incubation of spleen cells with SRBC coated with anti-SRBC IgG antibody in our experimental conditions. Our results show that the antigen specific immunoglobulin receptors are on the cells producing IgG antibody after primary or secondary immunization as well as on the IgM antibody-forming cells even in late stage of immune response.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology*; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006736:Horses / Q000276:immunology; D007114:Immunization; D008297:Male; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000939:Epitopes; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc