Double-stranded RNA dependent protein kinase (s) in rabbit reticulocyte lysates analogous to kinase from interferon-treated cells.
A double-stranded (ds) RNA-dependent protein kinase(s) from haemin-supplemented rabbit reticulocyte lysates in purified by chromatography on columns of poly(I).poly(C)-Sepharose. This method provides a more than 1000-fold purifications of the kinase(s) in a single step and a high yield. The purified kinase is responsible for the phosphorylation of (a) a polypeptide of molecular weight 67000, (b) the smallest subunit eIF-2 and (c) added histones. These kinase activities are comparable to the dsRNA-dependent protein kinase activities from interferon-treated cells.
D000818:Animals; D002847:Chromatography, Agarose; D007372:Interferons / Q000502:physiology; D007739:L Cells / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D011070:Poly I-C; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*
D012330:RNA, Double-Stranded; D007372:Interferons; D011494:Protein Kinases; D011070:Poly I-C