Purification and characterization of Tora-bean (Phaseolus vulgaris) lectin.
A lectin purified from the Tora-bean (Phaseolus vulgaris) by affinity chromatography with Con-A Sepharose was shown to be a glycoprotein containing 7.8% neutral sugars (D-mannose, N-acetyl-D-glucosamine, L-fucose, and D-xylose, in a molar ratio of 9.6 : 2.0 : 0.6 : 0.7). Its molecular weight was 130,000, as estimated by exclusion gel chromatography, and SDS gel electrophoresis showed that it consists of four subunits of molecular weight 32,000. The lectin reacts with various glycoproteins, i.e., blood group substances, human parotid salivary glycoprotein, fetuin, and bovine submaxillary mucin. Divalent cations, such as Ca2+, Mn2+, and Mg2+, appear to stimulate its reactivity. Inhibition tests using the glycopeptide fragment from fetuin and some oligosaccharides, as well as the binding test with 14C-N-acetyl-lactosamine suggest that the sequence of D-galactose, N-acetyl-D-glucosamine, and D-mannose residues in the carbohydrate chain of fetuin is essential for binding.
D000117:Acetylglucosamine / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D002118:Calcium / Q000494:pharmacology; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000302:isolation & purification*; D000509:alpha-Fetoproteins / Q000494:pharmacology
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D010446:Peptide Fragments; D010835:Phytohemagglutinins; D000509:alpha-Fetoproteins; D008345:Manganese; D008274:Magnesium; D002118:Calcium; D000117:Acetylglucosamine