Studies on corneal endothelial growth and repair. III. Effects of DNA and RNA synthesis inhibitors upon restoration of transparency following injury.
The effects of inhibition of DNA and RNA synthesis on the repair of corneal endothelial wounds has been studied. Hydroxyurea which stops DNA synthesis and mitosis in the endothelium does not prevent restoration of transparency. However, the endothelial cell density of healed wounds in animals receiving the drug is decreased. Actinomycin D, which blocks DNA-primed RNA synthesis, not only prevents mitosis but stops migration of cells into the wound focus as well. In this case the wound does not heal and the cornea does not regain transparency.
D000818:Animals; D002452:Cell Count; D003315:Cornea / Q000378:metabolism / Q000473:pathology; D065306:Corneal Injuries; D004247:DNA / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology*; D004727:Endothelium / Q000378:metabolism / Q000473:pathology; D006918:Hydroxyurea / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D011894:Rana pipiens; D014945:Wound Healing
D003609:Dactinomycin; D012313:RNA; D004247:DNA; D006918:Hydroxyurea