title: Anatomical relationship between opioid peptides and receptors in rhesus monkey brain.
abstract: To determine whether opioid peptide-receptor pharmacological association found in vitro (e.g., enkephalin-delta, dynorphin-kappa) predict anatomical relationships in situ, immunocytochemical and receptor autoradiographic studies were carried out on adjacent sections from the same brains of formaldehyde-perfused rhesus monkeys. Apparent mu and kappa opioid receptors (labeled, respectively, by [3H] naloxone and [3H]bremazocine under different incubation conditions), but not delta opioid receptors (labeled by [3H]D-Ala2, D-Leu5-enkephalin), survived the fixation procedure, and were found to be colocalized throughout the brain. We have observed complex associations between these binding sites and one, two, or all three opioid peptide systems (i.e., proopiomelanocortin, proenkephalin, and prodynorphin) in different brain regions. These multiple opioid peptide-receptor subtype associations are apparent, for example, in neural systems involved in the processing of pain stimuli, and may be important for mediating different types of analgesia. Since differential processing of proenkephalin and prodynorphin can give rise to opioids of varying receptor selectivities, the colocalization of opioid receptor subtypes may signify that such processing is a key regulatory event in determining which receptor subtype is activated and, thus, the physiological consequences of opioid neurotransmission.
mesh_terms: D000818:Animals; D001575:Benzomorphans / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping; D004399:Dynorphins / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism; D008253:Macaca mulatta; D009270:Naloxone / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D011869:Radioligand Assay; D011957:Receptors, Opioid / Q000378:metabolism*; D009435:Synaptic Transmission
chemical_list: D001575:Benzomorphans; D004723:Endorphins; D004745:Enkephalins; D011957:Receptors, Opioid; D009270:Naloxone; D011333:Pro-Opiomelanocortin; D004399:Dynorphins; C027248:bremazocine