Direct cloning and extended culture of antigen-specific MHC-restricted, proliferating T lymphocytes.
The development of antigen-specific, T-cell lines has been a subject of intensive investigation by many laboratories interested in the problems of the nature of the T-cell receptor and the mechanism of major histocompatibility complex (MHC) restriction. We describe here a modification of the soft agar cloning technique which has allowed us to rapidly generate and expand proliferating T-lymphocyte clones directly from murine lymph nodes. Using this technique, we demonstrate that such T-cell clones are antigen specific and require I--A compatible antigen-presenting cells for stimulation.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology*; D003470:Culture Media; D000939:Epitopes; D006241:Haptens; D007519:Isoantigens; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin
D003470:Culture Media; D000939:Epitopes; D006241:Haptens; D007519:Isoantigens; D010455:Peptides; D014373:Tuberculin