Ultrastructural demonstration of glucose 6-phosphatase in cerebral cortex.
A two-stage fixation technique has been developed to obtain morphological preservation and retention of glucose 6-phosphatase (G6-Pase) activity for its demonstration in rat cerebral cortex. The technique was then employed to localize the enzyme in the cortex where it produced a dense reaction over the well developed granular endoplasmic reticulum cisternae in nerve cells and oligodendrocytes which contrasted with a thin reaction in astrocytes. Other membranous organelles showed no reaction.
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology* / Q000648:ultrastructure; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D005952:Glucose-6-Phosphatase