title: Comparison between two chromogenic substrates for revealing an immunoperoxidase reaction of human metaphase chromosomes.
abstract: To study the binding of an antiadenosine serum to human chromosome DNA, two types of chromogenic reagents were compared. The procedure is as follows: lymphocytes with metaphase chromosomes were spread on slides; some slides were irradiated by UV; all slides were then incubated with antiadenosine rabbit serum and then with antirabbit sheep serum labelled with peroxidase; the latter was revealed in the classical manner by 3,3'-diaminobenzidine (DAB), or, alternatively, by p-phenylenediamine plus pyrocatechol (PPD-PC). The present study shows that the results obtained with PPD-PC were equivalent, if not superior, to those obtained with DAB. In the case of weaker reactions, results with PPD-PC were superior. Furthermore, this reagent has the major advantage of being noncarcinogenic.
mesh_terms: D015100:3,3'-Diaminobenzidine; D001560:Benzidines; D002396:Catechols; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects / Q000648:ultrastructure; D008677:Metaphase; D010655:Phenylenediamines; D013194:Staining and Labeling; D014466:Ultraviolet Rays
chemical_list: D001560:Benzidines; D002396:Catechols; D010655:Phenylenediamines; D015100:3,3'-Diaminobenzidine