The automated analytical electrophoresis microscope.
The components of an automated computer-controlled analytical electrophoresis microscope (AEMS) are described. Computer tracking of migrating cells projected under phase contrast onto a vidicon permits the rapid taking of multiple velocity measurements per cell so that reliable determinations of electrophoretic mobilities thereby result. The computer-controlled cell search and tracking algorithms allow high speed operation so that statistically valid profiles and data bases can be collected rapidly. Initial electrophoretic mobility evaluations have been carried out on populations of lymphocytes, erythrocytes, and platelets.
D000818:Animals; D001792:Blood Platelets / Q000032:analysis; D002462:Cell Membrane / Q000648:ultrastructure; D002645:Chickens; D004586:Electrophoresis / Q000379:methods*; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008838:Microcomputers; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D013499:Surface Properties; D014743:Videotape Recording