Inductive requirements for the generation of virus-specific T lymphocytes. II. Poxvirus and H-2 antigens associate without cellular or virus-directed protein synthesis, and remain immunogenic in cell membrane fragments.
The nature of the interaction between vaccinia virus (VAC) and fibroblastic cells that renders the latter capable of being recognized by virus-specific, H-2 identical murine T lymphocytes has been studied. L cells exposed for 10 min to VAC rendered noninfectious by exposure to ultraviolet light became susceptible targets for cytotoxic T lymphocytes (CTL) without the synthesis of new viral proteins. Susceptibility was retained even if cellular protein synthesis was irreversibly inhibited with pactamycin before virus exposure. Immobilization of cell-surface membranes by glutaraldehyde fixation before (but not after) exposure to virus severely impaired the formation of the "virus + self" complex that in vitro stimulated secondary CTL responses by H-2 identical virus-primed memory cells even though virus attachment to fixed cells were unaffected. This stimulatory complex, once formed, was maintained in membrane fragments prepared from cells previously exposed to VAC. These findings indicate that VAC-specific CTL or their immediate precursors can recognize only those viral envelope antigens that become membrane integrated and that this event requires neither host cell-specific nor virus-specific protein synthesis.
D000327:Adsorption; D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology; D005976:Glutaral / Q000494:pharmacology; D006183:H-2 Antigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010142:Pactamycin / Q000494:pharmacology; D010452:Peptide Biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology* / Q000528:radiation effects; D014764:Viral Proteins / Q000096:biosynthesis*
D000939:Epitopes; D006183:H-2 Antigens; D014764:Viral Proteins; D010142:Pactamycin; D005976:Glutaral