Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment.
In this pancreatic-glucagon-specific radioimmunoassay we used C-terminal-region-specific antiserum. OAL-123, produced against a 19-29 C-terminal fragment of porcine glucagon. On measurement of pooled plasma the ranges for intra- and inter-assay coefficients of variation were 4.8-8.1% and 7.5-10.7%, respectively. The concentration of immunoreactive glucagon in plasma of healthy subjects, as measured with the OAL-123 assay system, was 87.9 (SD 23.8) ng/L. Measurement of the same plasma samples with the 30K assay system (30K being an antiserum highly specific for pancreatic glucagon) showed a comparable value, 86.2 (SD 26.3) ng/L. We followed changes in human and dog plasma immunoreactive glucagon concentrations on arginine infusion and after glucose load, using the OAL-123 and the 30K assay systems, with identical results. Combining other results of comparative immunochemical characterization of the OAL-123 and 30K assay systems, we confirmed that the antisera raised against the C-terminal fragment of glucagon can be used in radioimmunoassay of pancreatic glucagon.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000276:immunology; D007611:Aprotinin / Q000494:pharmacology; D001120:Arginine; D001550:Benzamidines / Q000494:pharmacology; D002850:Chromatography, Gel; D004285:Dogs; D004492:Edetic Acid / Q000494:pharmacology; D016670:Gabexate; D005934:Glucagon / Q000097:blood*; D005951:Glucose Tolerance Test; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D010446:Peptide Fragments; D011863:Radioimmunoassay / Q000379:methods
D000596:Amino Acids; D000906:Antibodies; D001550:Benzamidines; D006146:Guanidines; D010446:Peptide Fragments; D016670:Gabexate; D005934:Glucagon; D007611:Aprotinin; D001120:Arginine; D004492:Edetic Acid; C032157:benzamidine