Sequential expression of functions during macrophage differentiation in murine bone marrow liquid cultures.
In the presence of a colony-stimulating factor, murine bone marrow cells proliferate and differentiate into macrophages. This culture system was taken as a model to study the expression of various functions by macrophages in the course of maturation. Several tests were performed daily and in parallel from the same batch of cells.  It was found that certain functions were expressed early and were also characteristic for mature macrophages such as Fc receptors, phagocytosis of latex beads and unspecific esterase activity. Other functions appeared and disappeared in an ordered sequence, such as the response to macrophage migration inhibitory factor and chemotactic factor as well as the production of interferon and of plasminogen activator. The time course of functional expression was strongly dependent on proliferation of precursor cells as well as proliferation of differentiated macrophages. It is suggested that the phenotypic expression of functions during differentiation is the basis for the functional heterogeneity of macrophages.
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D007372:Interferons / Q000096:biosynthesis; D007961:Leukocyte Migration-Inhibitory Factors / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D010960:Plasminogen Activators / Q000096:biosynthesis; D012397:Rosette Formation; D013997:Time Factors
D007961:Leukocyte Migration-Inhibitory Factors; D007372:Interferons; D010960:Plasminogen Activators