Acetoacetate utilization by human placental mitochondria.
It has been shown that mitochondria from human placenta incubated in the presence of 2-oxoglutarate or its precursors utilize acetoacetate at the rate about I nmol/min/mg protein. Utilization of acetoacetate is completely inhibited by arsenite. Mitochondria from human placental tissue show activity of 3-oxoacid-CoA transferase and acetoacetyl-CoA thiolase. It is proposed that acetoacetate utilization by placental mitochondria proceeds via the conversion to acetoacetyl-CoA catalysed by 3-oxoacid-CoA transferase, and then to acetyl-CoA, catalysed by acetoacetyl-CoA thiolase.
D000090:Acetoacetates / Q000378:metabolism*; D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D015256:Coenzyme A-Transferases; D005260:Female; D006801:Humans; D007656:Ketoglutaric Acids / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D013466:Sulfurtransferases / Q000378:metabolism
D000090:Acetoacetates; D007656:Ketoglutaric Acids; C016635:acetoacetic acid; D000101:Acetyl-CoA C-Acetyltransferase; D013466:Sulfurtransferases; D015256:Coenzyme A-Transferases; C021460:3-ketoacid CoA-transferase