Induction of RNA polymerase II activity in Junin virus-infected cells.
Nuclear involvement of the host cell in Junin virus (JV) replication was studied. alpha-Amanitin was shown to inhibit JV yield, and RNA synthesis in cell nuclei was found to be augmented during the eclipse period. JV infection exerted a selective effect on cellular RNA polymerase activities, when measured in purified infected nuclei. RNA polymerase I was inhibited, but RNA polymerase II was stimulated. These data support the hypothesis that RNA polymerase II is involved in arenavirus replication.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001116:Arenaviridae / Q000254:growth & development*; D002460:Cell Line; D002522:Chlorocebus aethiops; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004790:Enzyme Induction; D007668:Kidney; D012313:RNA / Q000096:biosynthesis; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development
D000546:Amanitins; D012332:RNA, Heterogeneous Nuclear; D012313:RNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I