Comparison of specific and cross-reactive antigens of alphaviruses on virions and infected cells.
Rabbit hyperimmune antisera against Sindbis (SIN) or Semliki Forest (SF) virus were absorbed with purified SIN virus or SIN virus-infected cells, or with SF virus or SF virus-infected cells. Residual antibody titers were determined by hemagglutination inhibition (HAI) and antibody-dependent, complement-mediated cytolysis (ADCMC) assays. It appeared that absorption with virus-infected cells removed ADCMC-detectable cross-reactive antibody much more efficiently than did absorption with either virus. HAI assays with the same absorbed antisera indicated that both virus and virus-infected cells removed HAI-detectable cross-reactive antibody. On the basis of these and other data, there appeared to be a cross-reactive antigen present on virus-infected cells which was detectable by ADCMC and was distinct from the cross-reactive antigen assayed by HAI.
D000818:Animals; D000914:Antibodies, Viral; D000920:Antibody-Dependent Cell Cytotoxicity; D000956:Antigens, Viral / Q000276:immunology*; D002478:Cells, Cultured / Q000382:microbiology; D002642:Chick Embryo; D003429:Cross Reactions; D000939:Epitopes; D006385:Hemagglutination Inhibition Tests; D012672:Semliki forest virus / Q000254:growth & development / Q000276:immunology*; D012845:Sindbis Virus / Q000254:growth & development / Q000276:immunology*; D014771:Virion / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes