The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins.
Application of immunocytochemistry to formalin-fixed, paraffin-embedded material tends to provide capricious results, even when employing the unlabelled antibody peroxidase-antiperoxidase (PAP) method. This study shows that treatment of tissue sections with pronase, a protease isolated from a strain of Streptomyces griseus, prior to performance of the PAP procedure greatly enhances its sensitivity. The optimum conditions for use of this enzyme in detecting intracellular immunoglobulins are given.
D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000276:immunology; D007124:Immunoenzyme Techniques; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007137:Immunoglobulin alpha-Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D011402:Pronase; D013194:Staining and Labeling
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007137:Immunoglobulin alpha-Chains; D007146:Immunoglobulin lambda-Chains; D011402:Pronase