Karyometric, cytophotometric, and histoenzymatic studies on neuroglia of young rats subjected to CCNU (Lomustine) action.
Studies on the effects of CCNU on neuroglia cells of the central nervous system in young individuals were conducted on 19 Wistar strain rats, each given 2 mg doses of 0.25 CCNU in 7th and 12th d of life. Karyometric and cytophotometric studies proved that CCNU administered to young rats induced changes in oligodendroglia and astroglia of the central nervous system. The changes included significant increase in nuclei contour and in the area of nuclei cross-section in oligodendro- and astroglia cells, elongation of oligodendroglia nuclei and an augmented DNA content in the 2 neuroglia types. The observed karyometric and cytophotometric changes were more evident in oligodendroglia nuclei than in astroglia nuclei.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D004247:DNA / Q000032:analysis; D005260:Female; D008130:Lomustine / Q000633:toxicity*; D008297:Male; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009836:Oligodendroglia / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D008130:Lomustine; D004247:DNA; D000469:Alkaline Phosphatase; D013834:Thiamine Pyrophosphatase