Effect of methisoprinol on E-rosette formation by trypsin-treated lymphocytes.
Human lymphocytes lose the capacity to form E rosettes after trypsin treatment. This ability is partially restored, in normal conditions, by a prolonged incubation in fresh medium supplemented with FCS. By adding methisoprinol to trypsin-treated lymphocytes, it has been observed a significant recovery after 2 hours of incubation, without further enhancement up to 24 hours, when compared to the controls. These results imply that the drug effect is time-dependent and that methisoprinol acts likely by a metabolic pathway in the early resynthesis of E receptors on trypsin-treated lymphocytes.
D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D012397:Rosette Formation; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism*
D007288:Inosine; D014357:Trypsin; D007542:Inosine Pranobex