title: A novel and comprehensive synthetic approach for the elucidation of protein antigenic structures. Determination of the full antigenic profile of the alpha-chain of human haemoglobin.
abstract: A comprehensive synthetic approach for the determination of continuous antigenic sites of proteins is presented. This approach consists of the synthesis of a series of consecutive overlapping peptides that, together, systematically represent the entire primary structure of the protein under study. Its application to the alpha-chain of human haemoglobin afforded, for the first time, a full profile of immunochemically active alpha-chain peptides and enabled the localization of all the major continuous antigenic sites of this haemoglobin subunit.
mesh_terms: D000941:Antigens; D000939:Epitopes; D006441:Hemoglobin A / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D008722:Methods; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology
chemical_list: D000941:Antigens; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D006441:Hemoglobin A