Rat liver epithelial cell cultures in a serum-free medium: primary cultures and derived cell lines expressing differentiated functions.
Rat liver epithelial cells explanted in a serum-free medium (SFM) composed of Ham's F10 basal medium plus free fatty acids adsorbed on bovine albumin gave successful rise to primary cultures and then to long-term cell lines that expressed liver functions; induction of L-tyrosine aminotransferase by glucocorticoids, hepatic pattern of progesterone metabolism, and biosynthesis of murine primary bile acids; chenodeoxycholic and cholic acid common to higher vertebrates and alpha-muricholic acid specific of the rat bile.
D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D004847:Epithelial Cells; D008099:Liver / Q000166:cytology* / Q000502:physiology; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D014444:Tyrosine Transaminase / Q000378:metabolism
D001647:Bile Acids and Salts; D003470:Culture Media; D011374:Progesterone; D003907:Dexamethasone; D014444:Tyrosine Transaminase