Two distinct allotypic determinants on the antigen-specific suppressor and enhancing T cell factors that are encoded by genes linked to the immunoglobulin heavy chain locus.
The alloantiserum was raised in BALB/c (H-2d, Igh-1a) mice hyperimmunized with spleen cells of Igh allotype congenic mice, CB-20 (H-2d, Igh-1b). It was found to define the new allotypic determinants (distinct from B cell Igh constant region determinants: Igh allotype) expressed only on a small population of T cells belonging to the Thy-1 dull-stained Lyt-2- or Lyt-2+ population of Igh-1b mice. Genes coding for the determinants were shown to be accommodated somewhere in the right side of the Igh variable region gene (Igh-V) cluster, as the antibody activity was completely absorbed with BAB-14 thymocytes. It was also demonstrated that the products detected by the antiserum represent the allotypic determinants (probably constant region determinants) on the antigen-binding moiety of the antigen-specific augmenting (TaF) and suppressor (TsF) T cell factors. Moreover, determinants on TsF were found to be distinct from those on TaF. Therefore, it can be suggested that the two genes coding for the T cell allodeterminants (distinct from those of the B cell Igh) are located in the right side of the B cell Igh-V on the 12th chromosome, and that both encode the antigen-recognition units of the functionally distinct T cell factors.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002874:Chromosome Mapping; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005815:Genetic Code; D008040:Genetic Linkage; D006433:Hemocyanins / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000235:genetics / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007375:Interleukin-1; D008222:Lymphokines / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000235:genetics / Q000276:immunology*; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007375:Interleukin-1; D008222:Lymphokines; D011506:Proteins; D013491:Suppressor Factors, Immunologic; D006433:Hemocyanins