title: Molecular cloning of human interferon cDNA.
abstract: A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979) Proc. Jpn. Acad. 55, Ser. B, 464-469]. This conclusion was confirmed by a hybridization-translation assay, using rabbit globin mRNA and its cDNA-containing plasmid as a control. Plasmid TpIF319 was used as probe and another recombinant plasmid, TpIF319-13, whose cDNA insert consists of about 800 base pairs, was isolated. Nucleotide sequence analysis of the cDNA revealed that the DNA in fact codes for human fibroblast interferon.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003062:Codon; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts; D006801:Humans; D007372:Interferons / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D014981:Xenopus / Q000378:metabolism
chemical_list: D003062:Codon; D012333:RNA, Messenger; D007372:Interferons