Electrically-evoked release of [3H]-histamine from the guinea-pig hypothalamus.
1 [3H]-histamine was taken up by slices of guinea-pig hypothalamus against a concentration gradient. 2 Electrical field stimulation of the superfused slices resulted in an increased efflux of radioactivity, the major part of which was shown to be associated with histamine by paper chromatography. 3 The evoked release of histamine was dependent on calcium ions in the superfusate and was increased by 56% when the frequency of stimulation was doubled from 5 to 10 Hz.
D000818:Animals; D004558:Electric Stimulation; D006168:Guinea Pigs; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release; D007031:Hypothalamus / Q000378:metabolism*; D066298:In Vitro Techniques; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D006632:Histamine