Suppressive effect of triethyllead on entry of proteins into the CNS myelin sheath in vitro.
Incorporation of [14C]leucine into the myelin sheath was studied in brain stem slices prepared from 22-day-old rats. Individual major myelin proteins were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. There was a time lag before incorporation of the label into proteolipid protein (PLP) and intermediate protein (IP) reached maximal rates. Labelling of basic proteins (BP) and Wolfgram proteins (WP) revealed a much shorter lag in entry. Appearance of radioactive proteins in the myelin sheath was significantly hampered by triethyllead (PbEt3) added to the incubation medium at micromolar concentrations. Inhibition values were highest in the case of PLP and were closely followed by the values for IP. BP and WP were less inhibited, although incorporation of these proteins into myelin was still suppressed more than was synthesis of total homogenate protein. Thus, myelin -forming cells seem to be unduly vulnerable to the toxin relative to the rest of the tissue. Furthermore, the results indicate an interference of PbEt3 with certain posttranslational process involved in furnishing of integral myelin proteins.
D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D007854:Lead / Q000494:pharmacology*; D004676:Myelin Basic Protein / Q000378:metabolism; D009185:Myelin Proteins / Q000378:metabolism*; D009186:Myelin Sheath / Q000187:drug effects* / Q000378:metabolism; D009942:Organometallic Compounds / Q000494:pharmacology*; D011510:Proteolipids / Q000378:metabolism; D051381:Rats
D004676:Myelin Basic Protein; D009185:Myelin Proteins; D009942:Organometallic Compounds; D011510:Proteolipids; C028312:Wolfgram proteins; D007854:Lead; C010360:triethyllead