Angiogenesis by capillary endothelial cells in culture.
Capillary endothelial cells cloned from bovine and human tissues were grown in long-term culture. In the presence of tumor-conditioned medium, capillary tubes formed when the cells became confluent. Each tube began as a longitudinal vacuole in one cell and then appeared to be extruded and connected from one cell to the next. Branches appeared, and a capillary network developed over a period of 2 to 3 weeks. By light and electron microscopy, the tubes were nearly identical to capillaries in vivo. These experiments: (1) Demonstrate angiogenesis in vitro; (2) Show that all the information necessary to develop an entire capillary in vitro can be expressed by one cell type; (3) Suggest a mechanism for lumen formation by capillaries in vivo; (4) Offer a possibility of distinguishing between direct and indirect angiogenesis factors.
D000818:Animals; D002196:Capillaries / Q000648:ultrastructure*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004727:Endothelium / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic
D003470:Culture Media