Effect of elevated dietary tryptophan on protein synthesis in rat liver.
This study investigated whether rats force-fed for three days an elevated (1%) tryptophan diet compared to a control (0.2% tryptophan) diet had changes in hepatic protein metabolism. Earlier, we showed that a single administration of L-tryptophan to fasted rats caused a rapid increase in hepatic protein synthesis. In the present study rats force-fed a high tryptophan diet for 3 days and killed the fourth morning had increased rats of hapatic protein synthesis, cytochrome P-450 and b5 activities, in vitro nuclear RNA release (cell sap and nuclear effects) and nuclear envelope nucleoside triphosphatases activity compared to animals force-fed the control diet. We noted little or no change in hapatic total polyribosomal aggregation patterns or plasma and hepatic free amino acid levels.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D015786:Cytochromes b5; D004032:Diet; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase; D009711:Nucleotides / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014364:Tryptophan / Q000008:administration & dosage / Q000494:pharmacology*
D003580:Cytochromes; D009711:Nucleotides; D012313:RNA; D014364:Tryptophan; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D010744:Phosphoric Monoester Hydrolases; D043583:Nucleoside-Triphosphatase