Detection of immune deposits in glomeruli: the masking effect on antigenicity of formalin in the presence of proteins.
Formalin is known to mask the antigenicity of immune deposits in glomeruli but not of surface immunoglobulins of isolated lymphocytes. We have shown in mice with experimental passive anti-GBM glomerulonephritis that formalin masks the antigenicity of GBM-bound immunoglobulins only if the tissue is fixed before sectioning. The presence of a high concentration of normal bovine serum during fixation of cryostat sections masks the antigenicity of immune deposits, whereas formalin alone has no obvious effect. The same results were obtained with human immunoglobulins (IgG, IgM and IgA) bound to tissue sections. Protease treatment with pepsin and trypsin restored the ability of the immunoglobulins to be stained. The masking effect seems to be due to extensive cross-linking of environmental proteins which prevents fluorescent conjugates reaching their antigens. Methods for detecting immunoglobulins in tissues must, therefore, take into consideration the influence of fixatives not only on epitopes but also on the environment in which the antigenic determinants are localised.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005404:Fixatives / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000494:pharmacology*; D005921:Glomerulonephritis / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology*; D051379:Mice; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000936:Antigen-Antibody Complex; D000939:Epitopes; D005404:Fixatives; D005557:Formaldehyde; D012710:Serum Albumin, Bovine