Cell lineage analysis by intracellular injection of fluorescent tracers.
Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.
D001562:Benzimidazoles; D006690:Bisbenzimidazole; D004475:Ectoderm / Q000166:cytology; D007865:Leeches / Q000196:embryology*; D008648:Mesoderm / Q000166:cytology; D008938:Mitosis; D009842:Oligopeptides; D011402:Pronase / Q000494:pharmacology; D012235:Rhodamines; D014966:Xanthenes
D001562:Benzimidazoles; D009842:Oligopeptides; D012235:Rhodamines; D014966:Xanthenes; C027024:rhodamine D peptide; D011402:Pronase; D006690:Bisbenzimidazole