Insulin stimulates the incorporation of 32Pi into ribonucleic acid in cultured sertoli cells.
Chronic treatment of cultured Sertoli cells with insulin, FSH, or testosterone does not affect the number of attached cells or the amount of total RNA or poly(A)+ RNA per culture dish. However, Sertoli cells cultured in the presence of insulin have a nearly 2-fold stimulated incorporation of 32Pi into poly(A)+ RNA. The other hormone treatments did not induce any stimulation over control values. The t1/2 of the total poly(A)+ RNA was determined in pulse-chase experiments. None of the hormone treatments affected the t1/2 (16 h) of the total poly(A)+ RNA. The incorporation of [3H]uridine and 32Pi into the total nucleotide pools and into some individual nucleotides was determined by high pressure liquid chromatography analysis of acid-soluble extracts. Insulin-treated cells had approximately a 1.5-fold increased specific activity of [3H]uridine or 32Pi into the total nucleotide pool and in ATP, GTP, and UTP pools. Insulin apparently stimulates the uptake of phosphorylation (or both) of free nucleosides.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology; D013997:Time Factors
D007328:Insulin; D010710:Phosphates; D012333:RNA, Messenger; D011061:Poly A; D013739:Testosterone; D012313:RNA; D005640:Follicle Stimulating Hormone