title: The effect of monoclonal antibodies against Escherichia coli type 1 pili and capsular polysaccharides on the interaction between bacteria and human granulocytes.
abstract: Monoclonal antibodies against Escherichia coli type 1 pili and the K13 capsular polysaccharide strongly influenced the interaction between human polymorphonuclear leucocytes (PMNL) and E. coli 06:K13:H1. Bacteria with type 1 pili, associated with the neutrophils, caused a metabolic activation but were not ingested. Addition of monoclonal antibodies to type 1 pili resulted in increased association, but also phagocytosis and metabolic activation of the granulocytes. Monoclonal antibodies against the K13 polysaccharide strongly stimulated phagocytosis, especially of the type 1 piliated bacteria, suggesting a synergistic effect between binding of type 1 pili and the Fc part of the capsular antibodies to the PMNL. Addition of D-mannose inhibited the opsonization of type 1 piliated E. coli 06:K13:H1 in the presence of both type 1 pilus antibodies and capsular antibodies. Monoclonal anti-idiotypic (anti-anti-capsular) antibodies reduced the association with the PMNL of the bacteria preopsonized with anti-capsular antibodies. The bacterial ingestion and the metabolic activation of the PMNL were also reduced, suggesting a role for anti-idiotypic antibodies in specific modulation of inflammation.
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D000907:Antibodies, Bacterial / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000942:Antigens, Bacterial; D016667:Bacterial Capsules; D004926:Escherichia coli / Q000276:immunology* / Q000502:physiology; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007130:Immunoglobulin Idiotypes; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1