Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotides.
We have synthesized a new medium, sulfhydrylcellulose, for affinity chromatography of mercurated polynucleotides. It is the product of reaction between aminoethylcellulose and N-acetylhomocysteine thiolactone. Sulfhydrylcellulose carries up to 90 mumol of SH groups/g and is inexpensive, easy to prepare, and stable. Because it binds mercurated RNA specifically and reversibly and exhibits no size discrimination, sulfhydrylcellulose should have wide applications.
D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D002846:Chromatography, Affinity / Q000379:methods; D003570:Cytidine Triphosphate; D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli / Q000201:enzymology; D007202:Indicators and Reagents; D009941:Organomercury Compounds / Q000302:isolation & purification*; D011119:Polynucleotides / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D013539:Simian virus 40
D007202:Indicators and Reagents; D009941:Organomercury Compounds; D011119:Polynucleotides; D012367:RNA, Viral; C032153:sulfhydrylcellulose; D012313:RNA; D003570:Cytidine Triphosphate; D002482:Cellulose; D012321:DNA-Directed RNA Polymerases