Synthesis of L-fucose in thyroid tissue.
A de novo pathway for L-fucose synthesis has been detected in porcine thyroid tissue. This system uses guanosine diphospho-alpha-D mannose as a precursor and forms guanosine diphospho-beta-L-fucose as product. The system seems similar to those reported by others to exist in microorganisms and plants in that the first step of the pathway involves a 4-keto sugar nucleotide intermediate. The first enzyme of the pathway, guanosine diphospho-alpha-D-mannose oxidoreductase has been purified 57-fold from crude extracts by virtue of its affinity for Blue Sepharose.
D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000302:isolation & purification; D002846:Chromatography, Affinity; D005643:Fucose / Q000096:biosynthesis*; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D013552:Swine; D013961:Thyroid Gland / Q000378:metabolism*
D006154:Guanosine Diphosphate Fucose; D005643:Fucose; D006155:Guanosine Diphosphate Mannose; D002237:Carbohydrate Dehydrogenases; C020562:GDPmannose dehydrogenase