T cell hybrids with arsonate specificity. I. Initial characterization of antigen-specific T cell products that bear a cross-reactive idiotype and determinants encoded by the murine major histocompatibility complex.
T cell hybrids have been constructed between the BW5147 thymoma cell line and A/J splenocytes from mice suppressed with the p-azophenylarsonte hapten. Three independently derived cloned lines have been chracterized. Each secretes or sheds a 62,000-dalton antigen-specific product bound by rabbit antisera directed against the arsonate cross-reactive idiotype. In addition, each of the antien-specific molecules contains determinants encoded within the I region of the murine major histocompatibility complex. Peptide mapping analysis indictes that, whereas these molecules are remarkably similar, each is individually distinct in primary structure. The availability of cloned T cell lines that produce antigen-specific idiotype-positive I region-containing products should facilitate a more thorough dissection of the interrelationships of T-B interctions in the arsonate idiotypic system.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics; D006822:Hybrid Cells / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000235:genetics; D008285:Major Histocompatibility Complex; D051379:Mice; D008805:Mice, Inbred A; D013601:T-Lymphocytes / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010132:p-Azobenzenearsonate