Human beta-globulin factors affecting growth of human lymphocytes and mouse fibroblasts.
Evidence is presented to show the existence in human beta-globulin (Cohn fraction III) of 2 growth-promoting factors that stimulate DNA synthesis and cell division in human lymphocytes and 3T3 B mouse fibroblasts. After extraction of human beta-globulins at pH 3.0 in 0.1 M NaCl followed by sieve chromatography on Sephadex, 2 distinct fractions were obtained containing the biological activity; one of polypeptide nature and molecular weight of approx. 10 000 Daltons and the other consisting of a homogeneous ribonucleic acid, as revealed by polyacrylamide gel electrophoresis. The above-mentioned factors appear to lack both cell and species specificity, since they are mitogenic agents for cells as diverse as mouse fibroblasts and human lymphocytes.
D000818:Animals; D001609:Beta-Globulins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008934:Mitogens; D013268:Stimulation, Chemical
D001609:Beta-Globulins; D008934:Mitogens