title: Sequence of the N-terminal half of Bacillus amyloliquefaciens alpha-amylase.
abstract: Bacillus amyloliquefaciens alpha-amylase (1,4-alpha-D-glucan glucanohydrolase. EC 3.2.1.1), which is commercially supplied as 'Bacillus subtilis alpha-amylase' does not cross-react immunologically with B. subtilis alpha-amylase. This enzyme (from B. amyloliquefaciens) was cleaved by treatment with CNBr into seven fragments. Peptide A was selected for sequence determination. It is the longest one, containing 185 amino acids (i.e. approx. 50% of the total molecule) and connects to the hexapeptide of the N-terminus. Its primary structure was aligned by use of various proteolytic enzymes. The sequence of amino acids 181-184 is identical with that of amino acids 14-17 of the alpha-amylase isolated from B. subtilis (except that amino acid 183 is asparagine rather than aspartic acid).
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000681:Amylases; D001407:Bacillus / Q000201:enzymology*; D001412:Bacillus subtilis / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D010446:Peptide Fragments / Q000032:analysis; D013820:Thermolysin; D014357:Trypsin; D000516:alpha-Amylases
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D000681:Amylases; D000516:alpha-Amylases; D014357:Trypsin; D013820:Thermolysin