title: Organization and dynamics of lipids in bovine brain coated and uncoated vesicles.
abstract: Three characteristics have been demonstrated by the chemical analysis of bovine brain coated vesicles following removal of the coat proteins: a high protein content, a high cholesterol/lipid ratio and a high percentage of phosphatidylethanolamine amongst the phospholipids. The study of lipid bilayer organization and dynamics has been performed using the fluorescent probes pyrene and parinaric acid (cis and trans). This has allowed the study of both lateral mobility and rotational motion in the lipid bilayer of the coated and uncoated vesicles. Lateral mobility in the fluid phase of the lipid is slightly reduced by the presence of the clathrin coat, as indicated by the lower diffusion coefficient of pyrene in coated compared with uncoated vesicles. At all temperatures from 6 degrees to 30 degrees C, solid-phase domains, probed by trans parinaric acid, coexist with fluid-phase domains in the lipid bilayer. The temperature dependence of the parinaric acid lifetimes and of their amplitudes strongly suggests that the solid phase domains decrease in size with temperature, both in coated and uncoated vesicles. However, the difference in the value of the anisotropy at long times (r infinity), between coated and uncoated vesicles (a difference which is more pronounced for cis than for trans parinaric acid), indicates that the presence of the clathrin coat introduces disorder in the surrounding lipids, thus suggesting a possible role of the clathrin in the formation of the pits on the plasma membrane.
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D002417:Cattle; D002462:Cell Membrane / Q000648:ultrastructure; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure*; D011992:Endosomes / Q000648:ultrastructure*; D007700:Kinetics; D008051:Lipid Bilayers; D008563:Membrane Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D013050:Spectrometry, Fluorescence
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids