Production of IFN beta with human fibroblasts in monolayer cultures. Comparative studies of induction with virus (NDV) and synthetic polynucleotides (poly I - poly C).
A comparative study of the production of IFN beta by human skin fibroblasts in monolayers was carried out using induction by NDV and poly I - poly C. The induction by polynucleotides produced significantly higher IFN beta titers. An attempt to grow those diploid cells on microcarriers resulted in a failure.
D000328:Adult; D002478:Cells, Cultured; D002675:Child, Preschool; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000096:biosynthesis* / Q000302:isolation & purification; D008297:Male; D009522:Newcastle disease virus / Q000276:immunology*; D011070:Poly I-C / Q000494:pharmacology*; D012867:Skin / Q000276:immunology
D007372:Interferons; D011070:Poly I-C