Experimental pancreatitis in the rat. The role of phospholipase A in sodium taurocholate-induced acute haemorrhagic pancreatitis.
Acute haemorrhagic pancreatitis was induced in rats by injecting 0.2 ml of 5% (92.9 mmol/l) aqueous solution of sodium taurocholate into the common biliopancreatic duct. Lysolecithin was separated from the pancreatic homogenate by thin-layer chromatography and quantified by phosphorus determination. The lysolecithin content increased rapidly, remained elevated for 12 h, and returned to the control level 24 h after the injection. Treatment with a trypsin inhibitor, aprotinin (Trasylol), given intraperitoneally and intravenously during 2 h postoperatively (800,000 units/kg of body weight) had no beneficial effects compared with physiological saline treatment. When the animals were treated similarly with a phospholipase A inhibitor, procaine hydrochloride (40 mg/kg of body weight), 45% of them survived 72 h (p less than 0.01). It was concluded that phospholipase A, which converts lecithin into lysolecithin, plays a significant role in the pathogenesis of acute pancreatitis.
D000208:Acute Disease; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D005260:Female; D006470:Hemorrhage / Q000139:chemically induced / Q000201:enzymology*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D013656:Taurocholic Acid
D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D011343:Procaine; D013656:Taurocholic Acid; D007611:Aprotinin; D010740:Phospholipases; D010741:Phospholipases A