title: Effect of fixation upon the histochemistry of glycosaminoglycans (mucopolysaccharides) in intraoral wounds.
abstract: Intraoral wounds and biochemical films were examined to determine the best method of fixation for glycosaminoglycans. Tissues fixed in 1% cetyl pyridinium chloride-10% formalin stained intensely with preparations of Alcian blue. When these tissues were treated with Streptomyces hyaluronidase and bacterial chondroitinases, staining was comparable to untreated tissues fixed in only 10% formalin. The addition of cetyl pyridinium chloride to formalin fixatives was a requirement for glycosaminoglycan histochemistry in open wounds, and presumably in other tissues that contain these complex sugars.
mesh_terms: D000423:Alcian Blue; D000818:Animals; D005404:Fixatives; D006025:Glycosaminoglycans / Q000032:analysis*; D006097:Granulation Tissue / Q000032:analysis; D006651:Histocytochemistry; D008297:Male; D009061:Mouth Mucosa / Q000032:analysis / Q000293:injuries*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005404:Fixatives; D006025:Glycosaminoglycans; D000423:Alcian Blue