Influence of synchronized sleep on the biosynthesis of RNA in neuronal and mixed fractions isolated from rabbit cerebral cortex.
Adult male rabbits implanted with dural electrodes were injected intraventricularly with [3H]orotate and killed 1 h later. During the period of incorporation they were left undisturbed while their EEG activity was continuously monitored. In the fraction of neuronal perikarya prepared from cerebral cortex by a method developed by Satake and Abe in 1966, the relative content of radioactive RNA of the nuclear particulate showed a twofold increase in the transition from 0 to 100% synchronization. On the other hand, a slight but significant decline was observed in the corresponding cytoplasmic compartment. A marked increase in the relative content of radioactive RNA was similarly observed in the nuclear particulate prepared from the mixed cellular fraction. The corresponding cytoplasmic compartment showed a nonsignificant increase. These results indicate that during sleep neuronal nuclei accumulate newly synthetised RNA (presumably hnRNA) at a faster rate. Under the same conditions the process of RNA transfer to the cytoplasm (presumably rRNA) may be reduced. These effects may be only partly shared by other cerebral cells.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D012313:RNA / Q000096:biosynthesis*; D011817:Rabbits; D012890:Sleep; D013997:Time Factors
D012313:RNA