[Improvement in the method of cultivating Halprowiae (Chlamydiae) in cell cultures].
Comparative evaluation of several methods increasing the effectiveness of cultivating Halprovia (7 strains of different origins) in L-929 and McCoy cell cultures has shown that the maximum effectiveness can be achieved by supplementing the standard method for the cultivation of obligate intracellular parasites with the method of forced adsorption of Halprovia on the monolayer and the treatment of cells with DEAE dextran or cycloheximide. The peculiarities of detecting cytoplasmic halprovian inclusions in infected cells by staining the specimens with fluorescent antibodies, with Lugol's solution, and according to May-Grünwald-Giemsa are described.
D000327:Adsorption; D002460:Cell Line; D002689:Chlamydia / Q000254:growth & development*; D003513:Cycloheximide / Q000494:pharmacology; D003637:DEAE-Dextran / Q000494:pharmacology; D007065:Idoxuridine / Q000494:pharmacology; D007739:L Cells; D008828:Microbiological Techniques; D013194:Staining and Labeling
D003637:DEAE-Dextran; D003513:Cycloheximide; D007065:Idoxuridine
Usovershenstvovanie metoda kul'tivnirovaniia gal'proviĭ (khlamidiĭ) v kul'ture kletok.