Separation of human X and Y spermatozoa by albumin gradients and Sephadex chromatography.
Two methods of separating X and Y spermatozoa in human sperm were evaluated. In the first method, the semen was laid over bovine serum albumin in order to obtain high proportions of Y spermatozoa. The mean number of Y spermatozoa increased from 52% to 74%. For the second method, the semen was passed through columns of Sephadex G50 in order to increase the proportion of X spermatozoa. The X spermatozoa increased from a mean of 60% to 74%. The degree of separation in individual samples showed considerable variation in both methods. In some samples only a 6% change in the proportion of X and Y spermatozoa could be obtained, whereas in others the increase was 37%. The proportion of motile spermatozoa showed a marked increase with the Sephadex method.
D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003911:Dextrans; D005260:Female; D006801:Humans; D008297:Male; D012710:Serum Albumin, Bovine; D012730:Sex Chromosomes; D012732:Sex Determination Analysis; D013081:Sperm Motility; D013094:Spermatozoa; D014960:X Chromosome; D014998:Y Chromosome
D003911:Dextrans; D012710:Serum Albumin, Bovine; C025614:sephadex