[Density-gradient centrifugation in lithium metatungstate and tris-(hydroxymethyl)aminomethane phosphotungstate].
New substances--lithium metatungstate (MTL) and tris-(hydroxymethyl)aminomethane phosphotungstate (PTT)--have been presented for density-gradient preparation. The buoyant densities of protein, RNA, DNA and some nucleoproteins were determined in solutions of these salts. Nucleic acids have been smaller buoyant density (1.1 g/cm3) than the proteins in contrast to CsCl-gradients. The protein in PTT solution have buoyant density 1.5 g/cm3 and in MTL solution 2.0-2,3 g/cm3. It was shown that MTL gradients allow to reach better resolution in nucleoprotein analysis than CsCl gradients.
D002499:Centrifugation, Density Gradient / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D007202:Indicators and Reagents; D008094:Lithium; D018020:Lithium Compounds; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification*; D010772:Phosphotungstic Acid / Q000031:analogs & derivatives; D011506:Proteins / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D014414:Tungsten; D017973:Tungsten Compounds
D007202:Indicators and Reagents; D018020:Lithium Compounds; D009698:Nucleoproteins; D011506:Proteins; D017973:Tungsten Compounds; D010772:Phosphotungstic Acid; C030001:lithium metatungstate; D012313:RNA; C030002:tris-(hydroxymethyl)aminomethane phosphotungstate; D004247:DNA; D008094:Lithium; D014414:Tungsten
Ravnovesnoi tsentrifugirovanie v gradientakh plotnosti metavol'framata litiia i fosforno-vol'framata tris-(oksimetil)aminometana.