title: Restriction map of poliovirus type 2 cDNA.
abstract: Poliovirus type 1 RNA was reverse-transcribed into c-DNA and inserted at the Pst I site of the plasmid vector pBR322 of E. coli. Resulting recombinant plasmids were analyzed by hybridization with RNase T1-resistant 32P-labeled oligonucleotides, and by restriction enzyme mapping. All of the genome was cloned in a series overlapping cDNA inserts, the longest of which was 3.2 kb. The restriction map of the poliovirus cDNA is presented.
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D012367:RNA, Viral; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes