Selective stimulation of the synthesis of an 80,000-dalton protein by calcium ionophores.
Brief exposure of cultured chicken pectoralis muscle cells to ionomycin or A23187 selectively increases the rate of incorporation of [35S]methionine into an 80,000-dalton protein was also observed upon cell-free translation of poly(A)-enriched RNA isolated from ionomycin-treated, as compared with control, cultures. These observations suggest that ionomycin selectively increases the cellular concentration of mRNA, which codes for the 80,000-dalton protein. The effect is probably mediated through an increase in cytoplasmic [Ca2+] caused by the ionophore. A similar effect of ionomycin was observed in cultured fibroblasts, HeLa cells, mouse LSP cells, and monkey kidney CV1 cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D002645:Chickens; D004987:Ethers / Q000494:pharmacology; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology*; D008565:Membrane Proteins / Q000096:biosynthesis*; D008970:Molecular Weight; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger
D000900:Anti-Bacterial Agents; D004987:Ethers; D007476:Ionophores; D008565:Membrane Proteins; D012333:RNA, Messenger; D011061:Poly A; D000001:Calcimycin; D015759:Ionomycin; D012313:RNA