5-Azacytidine induction of mouse endogenous type C virus and suppression of DNA methylation.
5-Azacytidine was found to induce the expression of BALB:virus-1 and BALB:virus-2 from K-BALB cells and ecotropic endogenous virus from AKR2B cells. Efficiency of the induction was high and comparable to that by 5-bromodeoxyuridine. The level of methylcytosine in newly synthesized DNA was drastically decreased when K-BALB cells were treated with 5-azacytidine. There was an inverse relationship between the level of DNA modification and the frequency of virus expression.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D003562:Cytidine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000096:biosynthesis*; D003841:Deoxycytidine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D012190:Retroviridae / Q000187:drug effects / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D003841:Deoxycytidine; D003562:Cytidine; D001973:Bromodeoxyuridine; D001374:Azacitidine