Two-dimensional gel electrophoresis can be used to examine chromatographic effluent fractions from displacement column chromatography.
We show how two-dimensional gel electrophoresis can be used to monitor protein components in effluent fractions from a displacement column. A minor protein in human serum, of interest in studies on psoriasis and highly enriched by using carboxymethyldextrans as displacers on DEAE-Sephacel, was easily detected in the effluent fractions with the two-dimensional gel method because its concentration was sufficiently high and there was no interference by the carboxymethyldextrans or salt.
D001798:Blood Proteins / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D011565:Psoriasis / Q000097:blood*
D001798:Blood Proteins; D003911:Dextrans; C014392:carboxymethyl dextran