A general interactive model for B cell activation II. Experimental verification.
The general interactive model described in the previous paper was subjected to experimental testing. We examined the high affinity anti-TNP plaque-forming cell response of cultures of murine lymphocytes exposed to lipopolysaccharide and trinitrophenylated lipopolysaccharide (TNP--LPS). From our model we predicted the effects of the addition of free LPS on the dose response to TNP--LPS, the effect of the addition of Polymyxin B to cultures stimulated with TNP--LPS, and the effect on the response to TNP--LPS preparations with different haptenation ratios. The results obtained were consistent with the predictions of the general interactive model.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004926:Escherichia coli; D005260:Female; D006241:Haptens; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008954:Models, Biological; D011112:Polymyxin B / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
D006241:Haptens; D008070:Lipopolysaccharides; D014301:Trinitrobenzenes; D011112:Polymyxin B