Immunological studies on cathepsins B and H from rat liver.
The immunological properties of thiol proteinases from rat liver, cathepsin B, H, and L, were examined. Rabbit antisera were prepared against cathepsins B and H from rat liver. Anti-cathepsin B and H ser quantitatively precipitated only the corresponding enzyme protein. On immunodiffusion analysis, anti-cathepsin B serum reacted with cathepsin B, but not with cathepsin H, indicating that the three thiol proteinases are distinct. Immunoelectrophoresis using a mixture of anti-cathepsin B and anti-cathepsin H showed that the precipitin line with cathepsin B moved faster to the anode than with cathepsin H. Immunological diffusion with antisera indicated that rat liver cathepsin B and H were immunologically identical with cathepsins B and H from rat kidney, lung, spleen, brain, and heart, but different from cathepsin B or H from human or rabbit liver.
D000818:Animals; D002401:Cathepsin B; D056655:Cathepsin H; D056668:Cathepsin L; D002403:Cathepsins / Q000276:immunology*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D000939:Epitopes / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008099:Liver / Q000201:enzymology*; D011233:Precipitin Tests; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014018:Tissue Distribution
D000939:Epitopes; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; C534280:Ctsl protein, rat; C534126:CTSH protein, human; D056655:Cathepsin H; C534128:Ctsh protein, rat