Comparative study of four HDL-cholesterol assay methods.
Four methods for HDL-cholesterol assay are compared using the plasma of 119 subjects. Results for the entire population and for subgroups defined by triglyceridemia and cholesterolemia levels are determined. The methods used are: ultracentrifugation, electrophoresis, dextran sulfate precipitation, and concanavalin A precipitation. The results indicate that ultracentrifugation and electrophoresis are not easily adapted to routine work, require large investments and do not separate the lipoproteins according to physiopathological criteria. The HDL values obtained with the different methods show significant differences, even though the correlations between the methods are highly significant. The precipitation methods are easiest to perform; however, the HDL-cholesterol values from dextran sulfate precipitation are higher than those from concanavalin A precipitation. The authors discuss the best choice for determining the atherogenic risks of hyperlipoproteinemia in clinical practice.
D001797:Blood Protein Electrophoresis; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood*; D003208:Concanavalin A; D016264:Dextran Sulfate; D003911:Dextrans; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
D003911:Dextrans; D008075:Lipoproteins, HDL; D014280:Triglycerides; D003208:Concanavalin A; D016264:Dextran Sulfate; D002784:Cholesterol