In vitro protein synthesis activity of poly(A) RNA from neuroblastoma cells.
Protein synthesis activity of poly(A) RNA from M1 neuroblastoma cells was studied in a reticulocyte cell-free system. The activity of poly(A) RNA from M1 cells "differentiated" morphologically by bromodeoxyuridine was compared with that of proliferating cells. Poly(A) RNA from differentiated cells was about 10% more active than the corresponding RNA from proliferating cells. The products synthesized in vitro were fractionated by polyacrylamide gradient gel electrophoresis. A 420,000-dalton band was present in the translation products programmed by differentiated cell poly(A) RNA; it was absent in the translation products programmed by proliferating cell poly(A) RNA.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002999:Clone Cells / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000032:analysis; D009447:Neuroblastoma / Q000032:analysis*; D011061:Poly A / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000378:metabolism*
D012334:RNA, Neoplasm; D011061:Poly A; D012313:RNA; D001973:Bromodeoxyuridine