Stable bone-marrow-derived cell line producing transforming avian acute leukemia virus OK 10.
A stable bone-marrow-derived cell line, OK-BM, producing transforming avian acute leukemia virus OK 10, was established. The cell line has been maintained in culture for over 4 years and 405 passages. The cells grow rapidly in suspension, have a low serum requirement, display a uniform morphology and perpetually release transforming OK 10 virus. No transforming helper virus was detected by interference assay. The cells have a chromosome complement of the chicken. Characterization of the cells indicates that they are of myeloid origin and have properties characteristic of proliferating transformed macrophages.
D000818:Animals; D001354:Avian Leukosis Virus / Q000254:growth & development*; D001854:Bone Marrow Cells; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D005260:Female; D008264:Macrophages / Q000473:pathology; D008297:Male; D009113:Muramidase / Q000032:analysis; D010587:Phagocytosis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013997:Time Factors
D012194:RNA-Directed DNA Polymerase; D009113:Muramidase