Nature of hapten-modified determinants involved in induction of T cell tolerance and suppressor T cells to NDFB contact sensitivity.
Unresponsiveness to DNFB contact sensitivity induced by DNP-modified lymphoid cells (DNP-LC) is mediated by two separable pathways: a rapidly induced, long lasting inhibition of reactive T cell clones (donor tolerance), and a transient period of suppressor T cell (Ts) activity. The present report has examined the nature of the hapten-modified determinants responsible for the induction of these pathways by utilizing soluble DNP-LC cell lysate preparations as tolerogens. The results indicate that both DNP-modified MHC and non-MHC encoded determinants can mediate donor tolerance 7 days after tolerization. On the other hand, the induction of Ts requires DNP-modified MHC determinants, since DNP-LC lysates passed over lentil lectin or specific anti-H-2 immunoabsorbent columns lost their ability to induce Ts. Additional experiments showed that the injection of DNP-LC lysate compatible with the recipient strain at the H-2K and H-2D region of the MHC was sufficient for the induction of Ts. We propose that Ts induction involves the direct presentation of DNP-H-2 determinants to Ts precursors, whereas the induction of donor tolerance may involve host processing and presentation of DNP-modified membrane determinants in conjunction with host MHC structures.
D000818:Animals; D003877:Dermatitis, Contact / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000276:immunology; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens; D006241:Haptens / Q000276:immunology*; D007108:Immune Tolerance; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*
D004136:Dinitrobenzenes; D000939:Epitopes; D006183:H-2 Antigens; D006241:Haptens; D037102:Lectins; D004139:Dinitrofluorobenzene