A cloned repeated DNA sequence in human chromosome heteromorphisms.
A sequence derived by ECoRI restriction of human satellite DNA III has been cloned in lambda gt WES. The cloned DNA was used as a template for in vitro synthesis of cRNA, which was hybridized in situ to preparations of human metaphase chromosomes with a range of heterochromatic polymorphisms. Most of the hybridization was found on chromosome 1, and the amount of hybridization was related to the size of the C-band on this chromosome. Hybridization to other chromosomes was not related to the C-band size, although hybridization of total satellite DNA is proportional to C-band size. Total satellite DNAs contain a mixture of sequences, some of which are predominantly located on only one pair of chromosomes. Hybridization in situ is able to discriminate between such chromosome-specific sequences and the bulk of satellite DNA. Further analysis of satellite DNAs may identify sequences specific for every chromosome pair.
D001483:Base Sequence; D002900:Chromosomes, Human, 1-3 / Q000648:ultrastructure; D002902:Chromosomes, Human, 16-18 / Q000648:ultrastructure; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure; D003001:Cloning, Molecular; D004261:DNA Replication; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics*; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012313:RNA / Q000235:genetics
D004276:DNA, Satellite; D006570:Heterochromatin; D012313:RNA