Separation of allelic variants by two-dimensional electrophoresis.
The resolving power of two-dimensional (2-D) gel electrophoresis has been tested using 17 allele products at five loci. Standard O'Farrell gels could separate 13 of these isozymes. The addition of a second pH gradient made it possible to separate all but one of the variant proteins. These results indicate that 2-D gel electrophoresis can resolve more than 90% of variants originally detected by one-dimensional (1-D) electrophoresis. The implications of these results for the low estimates of average heterozygosity obtained in surveys using 2-D gel electrophoresis are discussed.
D000483:Alleles; D000681:Amylases / Q000235:genetics; D000818:Animals; D002256:Carbonic Anhydrases / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D014644:Genetic Variation; D005914:Globins / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007525:Isoelectric Focusing; D051379:Mice; D008970:Molecular Weight; D012709:Serum Albumin / Q000235:genetics
D012709:Serum Albumin; D005914:Globins; D000681:Amylases; D002256:Carbonic Anhydrases