Hepatotoxicological studies of pyrazolone derivatives on rats. Part 3: Quantitative immunologic determination of serum concentration of transferrin, IgG, alpha 2-acute phase protein and VLDL.
At present no sufficiently sensitive liver function tests are available to exactly detect small liver impairments caused by drugs. In this respect immunological quantitation of serum proteins could be useful. We have determined quantitative alterations of four serum proteins (IgG, transferrin, alpha 2-AP = alpha 2-acute phase protein, VLDL = very low density lipoprotein) from rats treated with aminophenazone, phenazone and propyphenazone (each 1,55 mmol/kg body mass/d). The serum concentration of the four proteins examined is temporarily elevated immediately after onset of treatment. In chronic treatment transferrin is increased by the influence of all drugs investigated. Propyphenazone treatment increased the amount of VLDL whereas aminophenazone had the opposite effect, phenazone is without influence in the same period. The alpha 2-AP serum level is decreased in long time treatment, IgG remains unchanged. The observed alterations indicate disturbances of liver function in the investigated rats. Among the drugs used, aminophenazone has the strongest, propyphenazone the smallest liver damaging effect. The results reported support the importance of serum protein quantitation in connection with the search for possible liver damage due to drug action.
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood*; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis*; D008079:Lipoproteins, VLDL / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008111:Liver Function Tests; D011720:Pyrazoles / Q000633:toxicity*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014168:Transferrin / Q000032:analysis*; D000511:alpha-Macroglobulins / Q000032:analysis*
D007074:Immunoglobulin G; D008079:Lipoproteins, VLDL; D011720:Pyrazoles; D014168:Transferrin; D000511:alpha-Macroglobulins