Purification of alkaline phosphatase from the alimentary canal of Schistocerca gregaria (Forskal).
A method for the purification of alkaline phosphatase is described and some properties of the purified product determined. A 362-fold purification was obtained and enzyme is homogeneous as judged by SDS-gel electrophoresis. The molecular weight is 5.3 X 10(4) approximately, by disc-electrophoresis and also according to gel filtration and sucrose density-gradient.
D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004064:Digestive System / Q000201:enzymology; D006110:Grasshoppers / Q000201:enzymology*; D008970:Molecular Weight
D000596:Amino Acids; D000469:Alkaline Phosphatase