The effect of increasing glucose in CPD on the quality of stored blood.
Changes in quality of blood units containing one and a half or double amounts of glucose, stored at +4 degrees C for three weeks were analysed. An experimental preservative containing glucose and fructose (1 : 1) was also used. No other additives (purine or purine-nucleoside) were applied. A standard CPD preservative of the National Inst. of Haematology and Blood Transfusion was used as control. The pH, plasma free haemoglobin, K+ content, red blood cell (RBC) ATP and 2,3-DPG content, and RBC fragility index were determined in each sample. Increase of glucose concentration, the addition of fructose had a beneficial effect on blood pH, and on plasma free haemoglobin and K+ concentration. 150% glucose improved the 2,3-DPG maintenance in stored blood.
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000592:standards*; D003451:Cryoprotective Agents / Q000592:standards*; D004905:Erythrocyte Aging; D005632:Fructose; D005947:Glucose; D006801:Humans; D009996:Osmotic Fragility; D011188:Potassium / Q000097:blood
D003451:Cryoprotective Agents; D005632:Fructose; D000255:Adenosine Triphosphate; D005947:Glucose; D011188:Potassium