Karyotypes of the 5-azacytidine-sensitive and -resistant line of AKR mouse leukemia.
The karyotype of AKR mouse lymphoblastic leukemia cell line made resistant to 5-azacytidine was compared with the karyotype of parental line. In relation of 5-azacytidine-resistant line the parental cell have less chromosomes No. 5 and more of No. 17 per karyogram. The increased monosomy has been found in chromosomes No. 5, 6, 7, 8 and 18 in sensitive line and in chromosomes No. 1, 8 and 17 in 5-azacytidine-resistant cells. The highest occurrence of trisomy was present in the chromosomes No. 13 and 16 in both lines. In AKR sensitive cells 89% of karyograms displayed 3 to 13 markers per karyogram while in the resistant line there were only 37% of karyograms with 1 to 11 markers.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D004351:Drug Resistance; D005260:Female; D007621:Karyotyping; D007942:Leukemia, Experimental / Q000235:genetics; D007945:Leukemia, Lymphoid / Q000235:genetics*; D051379:Mice; D008806:Mice, Inbred AKR
D001374:Azacitidine