Human interferon for clinical trials: removal of pyrogen by a simple two-step procedure.
Commonly used preparations of human interferon are pyrogenic when used in humans for clinical trials. We describe a simple two-step procedure for the purification of human fibroblast interferon, employing Blue Sepharose chromatography and high-speed centrifugation. Such preparations are devoid of pyrogenic activity in a rabbit test system and are much more suitable for use in human clinical trials than previously used preparations.
D000818:Animals; D001681:Biological Assay / Q000379:methods; D001831:Body Temperature / Q000187:drug effects; D004340:Drug Contamination; D006801:Humans; D007372:Interferons / Q000032:analysis*; D008297:Male; D011749:Pyrogens / Q000302:isolation & purification*; D011817:Rabbits
D011749:Pyrogens; D007372:Interferons