Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for vicia faba.
A relatively straightforward approach is described to obtain differential contrast in sister chromatids for SCE detection in Vicia faba after BrdUrd incorporation. The hydrolysis time of the well-known Feulgen reaction was extended to differentially degrade the DNA, the BrdUrd-substituted strands being more resistant. The procedure may easily be adapted for other plant species with large chromosomes.
D001973:Bromodeoxyuridine / Q000494:pharmacology; D004396:Coloring Agents; D003434:Crossing Over, Genetic; D004247:DNA / Q000032:analysis; D007887:Fabaceae / Q000235:genetics; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D012394:Rosaniline Dyes; D012854:Sister Chromatid Exchange; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA; D001973:Bromodeoxyuridine