Rapid semi-quantitative isolation of beta-2-microglobulin from urine.
A rapid semi-quantitative purification of beta 2-microglobulin from transplant urine relies on a batch absorption using hydroxylapatite-cellulose. Stepwise elution is followed by Sephadex G-150 gel permeation chromatography. Using this methodology, it is possible to process quickly and conveniently many liters of urine at bench scale, with yields exceeding 95% and overall purity greater than 97% beta 2-microglobulin.
D000042:Absorption; D001609:Beta-Globulins / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D006801:Humans; D007158:Immunologic Techniques; D007668:Kidney / Q000378:metabolism; D016030:Kidney Transplantation; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008970:Molecular Weight; D001613:beta 2-Microglobulin / Q000302:isolation & purification* / Q000652:urine
D001609:Beta-Globulins; D001613:beta 2-Microglobulin