[Titration method for determination of carcinine (beta-alanyl-histamine). Application to the heart of Carcinus maenas (L.)].
1.--Carcinine was isolated by ion-exchange chromatography and titrated spectrophotometrically. The technique used was based on formation of an azo derivative of carcinine with sulfanilamide at basic pH. It allowed detection of carcinine at 1 microgram/ml concentration. 2.--Histamine could be detected simultaneously with the same technique, provided its concentration was greater than 1 microgram/ml. 3.--The mean amount of carcinine in cardiac tissues of Carcinus maenas was 22.1 micrograms/ml dry cardiac tissue. 4.--The technique described above did not allow detection of histamine in the heart of Carcinus maenas. If present, histamine must be at a concentration smaller than 0.1 microgram/ml dry cardiac tissue.
D000818:Animals; D003386:Brachyura; D002336:Carnosine / Q000031:analogs & derivatives / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004151:Dipeptides / Q000032:analysis*; D006632:Histamine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D004151:Dipeptides; D006632:Histamine; D002336:Carnosine; C015055:carcinine
Mise au point d'une méthode de dosage de la carcinine (beta-alanyl-histamine). Application au coeur de Carcinus maenas (L.).