On the mechanism of interaction of N-acetylphenylalanyl-tRNAPhe with ribosomes of Escherichia coli: effect of antibiotics and Tp psi pCpGp.
The tetranucleotide Tp psi pCpGp acts as a specific inhibitor of the rate of AcPhe-tRNAPhe binding in the ribosomal P site. This effect is observed both in the presence and in the absence of poly(U). In the absence of poly(U) antibiotics tetracycline and puromycin also decrease the rate of AcPhe-tRNAPhe binding. Some inhibition by tetracycline is observed with poly(U). All these inhibitors are known to be ligands of the ribosomal A site, and their influence on the P site binding can be most naturally explained by the suggestion that AcPhe-tRNAPhe enters the ribosome via the A site, forms there an intermediate complex, and spontaneous translocation into the P site follows. In the presence of poly(U) arguments in favour of this hypothesis are much weaker, but the same sequence of events is possible.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001665:Binding Sites; D004926:Escherichia coli / Q000378:metabolism; D008954:Models, Biological; D009841:Oligonucleotides / Q000494:pharmacology*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D011072:Poly U / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; C006383:tRNA, N-acetylphenylalanine-; D011072:Poly U; D010649:Phenylalanine; D011691:Puromycin; C029375:ribosylthymine phosphate-pseudouridine phosphate-cytidine phosphate-guanosine phosphate; D013752:Tetracycline