Pinocytosis of fluorescein-labelled growth-promoting alpha-globulin in human diploid cells.
Fluorescein isothiocyanate conjugated growth-promoting alpha-globulin (FITC-GPAG) binds to human diploid cells LEP 19 at 37 degrees C. After the initial binding step, labelled GPAG is progressively internalized by the cells. FITC-GPAG is present in pinocytic vesicles localized in the perinuclear region of the cells and its pinocytosis occurs without cell surface redistribution of GPAG.
D000510:Alpha-Globulins / Q000378:metabolism*; D002478:Cells, Cultured / Q000502:physiology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D010873:Pinocytosis; D013861:Thiocyanates
D000510:Alpha-Globulins; D005452:Fluoresceins; D006133:Growth Substances; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate