Studies of the producer cell of herpes simplex virus-induced interferon in mouse spleen cell cultures.
The producer cell of type I interferon was studied in spleen cell cultures of C57BL/6 mice stimulated by inactivated Herpes Simplex Virus (HSV). Interferon production was not abolished by pretreatment of the spleen cells by anti-theta serum plus complement. The producer cell of interferon was not removed by plastic adherence and was not destroyed by the addition of silica. It was present in spleens of 3 day old C57BL/6 mice and in spleens of nu/nu mice. It was not inactivated by treatment of nu/nu spleen cells by anti-theta serum plus complement. HSV-induced interferon production was abolished by passage of the spleen cells through nylon wool columns and by irradiation (1000 R) of the spleen cells. Collectively these data suggest that in murine spleen cell cultures type I interferon is produced by B cells. However, our data do not allow to rule out that the interferon producing cell may be an immature macrophage or an immature T cell.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation; D002478:Cells, Cultured; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D018139:Simplexvirus; D013154:Spleen / Q000166:cytology* / Q000528:radiation effects
D000961:Antilymphocyte Serum; D007369:Interferon Inducers; D007372:Interferons