Murine lymphocyte alloantigens. I. The Ly-6 locus.
The Ly-6 locus and the Ly-6.2 specificity have been previously described, and we now further define this locus and the allelic specificities, Ly-6.1 and Ly-6.2. Back-cross studies and examination of several recombinant inbred (RI) lines demonstrate that Ly-6 is distinct from other loci determining CMAD, except for ALA-1 and evidence is presented for the identity of Ly-6 and ALA-1. The Ly-6 congenic strain C3H.B6-Ly-6b is described and was used to prepare antisera in combinations congenic for Ly-6. The allelism of the Ly-6.1 and Ly-6.2 specificities was confirmed by the reactivity of these antisera with a segregating F2 generation. Antisera can be prepared between the Ly-6 congenic strains, although the magnitude of the response is under the control of one or more genes in A strain, not linked to H-2 or Ly-4 loci. Absorption analysis using separated T and B lymphocyte populations demonstrate that Ly-6.2 does not have exclusive peripheral T cell representation as originally reported, but is also present on B cells in amounts one-fourth to one-eighth of that found on T cells and is found in greater quantities on T blast cells.
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D000939:Epitopes; D006462:Hemolytic Plaque Technique; D007106:Immune Sera / Q000494:pharmacology; D007075:Immunoglobulin M; D007519:Isoantigens; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics*; D008810:Mice, Inbred C57BL; D011817:Rabbits; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D007106:Immune Sera; D007075:Immunoglobulin M; D007519:Isoantigens