title: Comparison of direct wet mount and trichrome staining techniques for detecting Entamoeba species trophozoites in stools.
abstract: Simulated freshly passed stools containing motile Entamoeba moshkovskii trophozoites were examined by direct wet mount and permanent (Trichrome) staining techniques. The percentage of detection by direct mount was 4.8%. The percentage of detection of the trophozoites by direct mount plus permanent stain was 58.5%. Laboratorians should be cautioned not to rely solely on the direct wet mount for detection or identification of protozoan trophozoites.
mesh_terms: D000818:Animals; D001391:Azo Compounds; D004396:Coloring Agents; D004747:Entamoeba / Q000145:classification / Q000302:isolation & purification*; D004801:Eosine Yellowish-(YS); D005243:Feces / Q000469:parasitology*; D008739:Methyl Green; D010274:Parasitology / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)