Genetic analysis of ad-3 mutants induced by AF-2 and two other nitrofurans in Neurospora crassa.
Our previous studies have shown that the nitrofurans AF-2, SQ18506, and FANFT are potent mutagens in Neurospora crassa. The genetic damage produced by these chemicals at the ad-3 region in N crassa has been characterized by a series of genetic tests. The results of these tests indicate that all three agents induce a high frequency of point mutations and probably a low frequency of multilocus deletions. A comparison of the complementation patterns among the AF-2--induced ad-3B mutants and those induced by other chemical agents indicates that the spectra of intragenic alterations induced by AF-2 in N crassa are similar to those induced by monofunctional alkylating agents.
D013184:5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole / Q000494:pharmacology; D000225:Adenine / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects; D005200:FANFT / Q000494:pharmacology; D005668:Furylfuramide / Q000494:pharmacology; D005816:Genetic Complementation Test; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D009581:Nitrofurans / Q000494:pharmacology*
D009581:Nitrofurans; D005668:Furylfuramide; D013184:5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole; D005200:FANFT; D000225:Adenine