Antiviral and cell multiplication inhibitory activities of mouse interferon poreparations tested on an interferon sensitive murine sarcoma cell line.
The cell multiplication inhibitory effect of SDS-treated mouse interferon separated into antiviral (AV) and cell multiplication inhibitory (CMI) fractions was compared to that of untreated similar interferon on a line of murine osteosarcoma cells. The untreated interferon poreparatin and the CMI fractions dose-dependently inhibited the multiplication of the cells as measured by cell count and incorporation of 3H-thymidine into the cultures. The AV fractions, containing comparable antiviral activites as the untreated interferon preparations, had only a minor effect on cell multiplication. The biochemical properties of the fractions studied remain unknown.
D000818:Animals; D001859:Bone Neoplasms / Q000188:drug therapy; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002850:Chromatography, Gel; D007372:Interferons / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D012516:Osteosarcoma / Q000188:drug therapy; D012513:Sarcoma, Experimental / Q000188:drug therapy*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
D012967:Sodium Dodecyl Sulfate; D007372:Interferons