Interferon enhances the expression of epstein-Barr virus early antigen in Daudi cells.
Treatment of the Burkitt lymphoma-derived cell line Daudi with highly purified human interferon-alpha (IFN-alpha) increased up to 100-fold the number of cells expressing Epstein-Barr virus (EBV)-determined early antigen (EA) without inducing the synthesis of virus capsid antigen (VCA), a late virus function. The increase in the number of EA-positive cells was proportional to interferon concentration up to 10(4) international units (IU)/ml. Treatment of Daudi cells with the same number of units of either partially purified (sp. act. 10(6) IU/mg protein) or electrophoretically pure (sp. act. 2 x 10(8) IU/mg protein) IFN-alpha both gave a similar increase in EA expression, strongly suggesting that the effects observed were indeed due to interferon. Furthermore, treatment of relatively interferon-insensitive Raji cells with IFN-alpha (1 to 10(4) IU/ml) had no significant effect on either spontaneous or 5-iodo-2'-deoxyuridine (IdUrd)-induced expression of EA or VCA. Human IFN-beta also enhanced the expression of EBV EA in Daudi cells. In contrast, when the latent EBV present in Daudi cells was activated to enter into a replicative cycle, either by induction with IdUrd or by superinfection with the non-transforming P3HR1 strain of EBV, then treatment with human IFN-alpha caused a marked inhibition of EA expression. Our results suggest that interferon can exert a differential action on virus replication depending upon the state of the virus genome within the cell.
D000956:Antigens, Viral; D036022:Capsid Proteins; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D014779:Virus Replication
D000956:Antigens, Viral; D036022:Capsid Proteins; D007372:Interferons; D007065:Idoxuridine