Is histone H5 present in mammals?
An extraction procedure for histone H5 for chicken erythrocytes described in the literature has been applied to mouse spleen. The SDS polyacrylamide gel electrophoresis pattern of the resulting protein preparation revealed the presence of a component with the mobility of the marked chicken erythrocyte H5. Additionally the preparation has been characterized using antiserum raised against purified chicken H5. The presumptive mouse spleen H5 preparation gave visible precipitation lines with the anti-H5 chicken-antiserum. The combined electrophoretic and immunological evidence suggests the presence of histone H5 in mammalian tissue.
D000818:Animals; D005075:Biological Evolution; D002645:Chickens / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000032:analysis* / Q000097:blood / Q000276:immunology; D005779:Immunodiffusion; D051379:Mice; D013045:Species Specificity; D013154:Spleen / Q000032:analysis*
D000939:Epitopes; D006657:Histones