Staining of rat thyroid parafollicular (C-) cells with the Sevier-Munger silver technique.
The argyrophil silver stain of Grimelius and of Sevier-Munger was studied in rat thyroid parafollicular (C-) cells after formation and Bouin fixation. The strongest and most easily reproducible staining reaction was obtained in tissue fixed with Bouin's fluid and stained by the Sevier-Munger technique. The identify of the argyrophil cell as the calcitonin-containing C-cells was established by re-staining the same sections with calcitonin antibodies according to the method of Sternberger.
D000818:Animals; D000906:Antibodies; D002116:Calcitonin / Q000276:immunology; D006651:Histocytochemistry; D007120:Immunochemistry; D008297:Male; D051381:Rats; D012834:Silver; D013194:Staining and Labeling; D013961:Thyroid Gland / Q000166:cytology*
D000906:Antibodies; D012834:Silver; D002116:Calcitonin