title: Polyadenylated RNA of Volvox: isolation and partial characterization.
abstract: Polyadenylated RNA from Volvox carteri has been isolated and partially characterized. Electrophoretic profiles of total cellular poly(A)-associated RNA of Volvox spheroids indicate a hetero-disperse distribution of size classes with the range extending from an apparent sedimentation value of approximately 10S to greater than 38S. The radioactive labelling kinetics of this material are typical for rapidly-turning-over RNA. The profiles of poly(A) RNA from different cell types show marked differences in average migration rate. Terminally-differentiated somatic cells contain a greater proportion of material of higher molecular weight than either gonidia (germ cells) or cleaving embryos. The poly(A) segments associated with cellular RNA, obtained by selective RNase digestion are heterogeneous in size as determined by gel electrophoresis with the largest tracts estimated to be 75-80 nucleotides long. Gonidia and embryos display the greatest degree of size heterogeneity, while somatic cells show predominantly the largest classes of poly(A) tract. It is apparent that gross changes in poly(A) RNA metabolism accompany development and cellular differentiation in Volvox.
mesh_terms: D002499:Centrifugation, Density Gradient; D000460:Chlorophyta / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis*; D012313:RNA / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D011061:Poly A; D012313:RNA