Identification by high-performance liquid chromatography of immunoreactive substance P released from isolated rat spinal cord.
An attempt was made to identify the immunoreactive substance P (SP) released from isolated rat spinal cords using reversed-phase high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA) for SP. Soaking the spinal cords of newborn rats in Krebs solution containing 90 mM K+ evoked a release of immunoreactive SP as well as of GABA and glycine in a calcium-dependent manner. Capsaicin also evoked a release of immunoreactive SP but not of GABA and glycine. The immunoreactive SP released from rat spinal cords by high K+ or capsaicin was analyzed by HPLC. A single peak was detected by RIA whose elution position coincided with that of synthetic SP.
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005971:Glutamates / Q000032:analysis; D005998:Glycine / Q000032:analysis; D011188:Potassium / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D013116:Spinal Cord / Q000032:analysis*; D013373:Substance P / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D005971:Glutamates; D013373:Substance P; D005680:gamma-Aminobutyric Acid; D011188:Potassium; D002211:Capsaicin; D002118:Calcium; D005998:Glycine