Production and detection of monoclonal anti-idiotype antibodies directed against a monoclonal anti-beta-adrenergic ligand antibody.
A new method has been developed to raise monoclonal anti-idiotypic antibodies. Monoclonal anti-idiotypic antibodies were obtained by fusion of NS-1 myeloma cells with splenocytes of mice immunised by intravenous injections of fixed hybridoma cells bearing a monoclonal antibody specific for beta-adrenergic ligands. New screening tests were developed to analyse the resulting hybridoma supernatants for different anti-idiotypic properties. Among 23 hybridoma supernatants recognising the idiotype, 6 were found to inhibit hapten binding and 3 of these recognised beta-adrenergic receptors.
D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D008024:Ligands; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology; D011943:Receptors, Adrenergic, beta / Q000032:analysis*
D000319:Adrenergic beta-Antagonists; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D008024:Ligands; D011943:Receptors, Adrenergic, beta