[Countercurrent distribution of L line monolayer and suspension cell cultures in a 2-phase polymer system].
The distribution of two sulines of L cells, differing in adhesive properties (monolayer and suspension cultures), have been investigated using polymer two-phase system dextran-500-polyenthylenglycol-600. By counter-current distribution it has been shown that most of the cells growing in monolayer have low coefficients of partition, unlike cells grown in suspension having a high coefficient of partition. It is supposed that differences revealed in properties of the cell surface may be associated with the ability of cells to form adhesive contacts.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003377:Countercurrent Distribution; D003470:Culture Media; D003911:Dextrans / Q000494:pharmacology*; D007739:L Cells / Q000166:cytology*; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013499:Surface Properties
D003470:Culture Media; D003911:Dextrans; D011092:Polyethylene Glycols
Protivotochnoe raspredelenie kletok monosloĭnoĭ i suspenzionnoĭ kul'tur linii L v dvukhfaznoĭ polimernoĭ sisteme.