Pregnancy-associated plasma protein-A: an inhibitor of the complement system.
Pregnancy-associated plasma protein-A (PAPP-A) was purified to homogenicity from third-trimester pregnancy plasma using ion exchange chromatography and affinity chromatography on con A-Sepharose and anti-human serum-Sepharose. The elution of PAPP-A, alpha 2-macroglobulin, Cl-inhibitor and alpha 1-antitrypsin was followed by immuno-diffusion. the eluates were tested for stimulation or inhibition of complement-induced haemolysis. PAPP-A markedly inhibits the haemolytic activity of complement.
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003169:Complement Inactivator Proteins; D004305:Dose-Response Relationship, Drug; D005260:Female; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D005779:Immunodiffusion; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011266:Pregnancy-Associated Plasma Protein-A / Q000302:isolation & purification / Q000494:pharmacology*
D003169:Complement Inactivator Proteins; D011257:Pregnancy Proteins; D011266:Pregnancy-Associated Plasma Protein-A