A new internal perfusion method for transport studies in squid giant axons.
A new internal perfusion method has been developed which allows control of the internal solute composition in squid axons. The superiority of this technique compared to the old perfusion methods is shown by the experiments performed which have reproduced, both qualitatively and quantitatively, the Na+ and Ca2+ fluxes observed in intact and dialyzed axons. Compared with the internal dialysis, the perfusion method has the advantage that the permeability barrier give by the porous capillary has been eliminated. This allows the introduction into the axon of solutes with very high molecular weight, at the same time that a fast and reliable internal control can be achieved.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D049832:Decapodiformes; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D012623:Seawater; D012964:Sodium / Q000378:metabolism
D000255:Adenosine Triphosphate; D012964:Sodium; D002118:Calcium