Proliferation of PPD-stimulated lymphocytes measured by sister chromatoid differential staining.
Lymphocyte proliferation in response to tuberculin-purified protein derivative (PPD) stimulation was measured by bromodeoxyuridine incorporation followed by sister chromatid differential staining PPD-stimulated lymphocytes responded identically to phytohaemagglutinin (PHA) stimulated lymphocytes in that there was a steady increase in the number of second- and third-division metaphases with a corresponding decrease in the number of first-division metaphases. However, the mitotic index was considerably less in the PPD-stimulated cultures. The response of PPD-stimulated lymphocytes was largely the result of repeated divisions of a small number of lymphocytes. Donors who had been treated for tuberculosis showed greater numbers of second- and third-division metaphases than donors who had received BCG vaccination. Third-division metaphases than donors who had received BCG vaccination. Third-division metaphases were also present in the PPD and PHA 48-hr cultures, indicating tha lymphocytes can both respond to stimulation and divide faster than was previously recognized.
D002455:Cell Division; D002842:Chromatids; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008677:Metaphase; D008940:Mitotic Index; D010835:Phytohemagglutinins / Q000494:pharmacology; D013194:Staining and Labeling; D014373:Tuberculin / Q000494:pharmacology*
D010835:Phytohemagglutinins; D014373:Tuberculin