title: Properties of purified ribonuclease P from Escherichia coli.
abstract: The purified protein moiety of ribonuclease P (EC 3.1.26.5) from Escherichia coli, a single polypeptide of molecular weight approximately 17 500, has not catalytic activity by itself on several RNA substrates. However, when it is marked in vitro with an RNA species called M1 RNA, RNase P activity is reconstituted. The rate at which the purified RNase P cleaves any particular tRNA precursor molecule depends on the identity of that tRNA precursor.
mesh_terms: D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D007700:Kinetics; D008970:Molecular Weight; D012313:RNA; D012343:RNA, Transfer; D043262:Ribonuclease P; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D029968:Escherichia coli Proteins; D012313:RNA; D012343:RNA, Transfer; D004720:Endonucleases; D012260:Ribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli