title: Demonstration of substance P in aortic nerve afferent fibers by combined use of fluorescent retrograde neuronal labeling and immunocytochemistry.
abstract: Combined use of the intraaxonal retrograde transport of the fluorescent marker 'true blue' with substance P (SP) immunocytochemistry has been used to trace the nodose ganglion projections of SP-containing neurons of the aortic depressor nerve. It has been found that (1) SP immunoreactive (SP-I) cell bodies are clearly demonstrable in clusters in the rostral part of the nodose ganglion without the aid of colchicine pretreatment; (2) 'true blue' is retrogradely transported to the nodose ganglion following its application to the central cut end of the aortic nerve; (3) 'true blue' fluorescence and SP fluorescent immunoreactivity can be visualized in the same tissue section and certain cell bodies in the nodose ganglia contain both SP-I and retrogradely transported 'true blue'. These results indicate that the aortic nerve which projects from the aortic arch baro- and/or chemoreceptors to brainstem vasomotor centers contains SP-I afferent fibers which emanate form the nodose ganglion.
mesh_terms: D000344:Afferent Pathways / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000294:innervation*; D005453:Fluorescence; D006651:Histocytochemistry; D007120:Immunochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009620:Nodose Ganglion / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism*
chemical_list: D013373:Substance P