Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin.
A cDNA library from membrane-bound poly(A)+ mRNA of developing potato tubers was constructed and two classes of essentially full-length cDNA clones for a precursor to the major tuber glycoprotein, patatin were isolated. Sequence analysis shows that the two classes are approximately 99% homologous and correspond to the two major species of patatin identified previously by NH2-terminal amino acid sequence analysis. Sequence analysis also predicts that patatin is synthesized with a 23 amino acid signal sequence. Northern blot analysis shows that patatin mRNAs are 1550 +/- 50 nucleotides in length and are normally not present in polyribosomal or total RNA from stems or leaves.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D005796:Genes; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger; C043222:patatin protein, Solanum tuberosum; D011061:Poly A; D004247:DNA; D002265:Carboxylic Ester Hydrolases; D004262:DNA Restriction Enzymes