Proliferation kinetics of perturbed cell populations determined by the bromodeoxyuridine-33258 technique: radiotoxic effects of incorporated [3H]thymidine.
A simple analytical method is described for the evaluation of flow cytometric data of perturbed cell populations obtained by applying the BrdUrd-33258 Hoechst technique. This procedure allows for determining the growth curve, the efflux from and the influx into G2 + M as well as the transit characteristics of cell cohorts through the G2 + M phase. As an example of the application of the method, the radiotoxic effects of incorporated 3H-thymidine on the proliferation of L-929 cells in vitro are described.
D000818:Animals; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D007399:Interphase / Q000528:radiation effects*; D007700:Kinetics; D007739:L Cells; D051379:Mice; D008938:Mitosis / Q000528:radiation effects*; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D004247:DNA; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole; D013936:Thymidine