Detection of luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60.
Differentiation of HL-60 cells toward the eosinophilic series has not been reported previously. Eosinophil granule specific staining with Luxol-fast-blue was used to determine if HL-60 cells could differentiate into the eosinophilic lineage. The specificity of the Luxol-fast-blue stain for cells of the eosinophilic series was substantiated by comparison of the staining of cells from a patient with an eosinophilic syndrome by Wright-Giemsa and Luxol-fast-blue. Luxol-fast-blue positivity was most notable in cells found in colonies formed from HL-60 clonogenic cells in semisolid agar medium. Colony and cluster formation was spontaneous but in the presence of medium conditioned by either human placental cells or the human monocyte-like cell line, GCT, Luxol-fast-blue positive colonies and clusters were detected at a higher frequency.
D002460:Cell Line; D004396:Coloring Agents; D004804:Eosinophils / Q000473:pathology*; D006801:Humans; D007211:Indoles; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000473:pathology*; D013194:Staining and Labeling
D004396:Coloring Agents; D007211:Indoles; C018588:Luxol Fast Blue MBS