Actin acetylation in Drosophila tissue culture cells.
In a permanent cell line derived from Drosophila embryos, cytoplasmic actin is produced as an unstable precursor, which is subsequently converted to a stable form. This conversion results in a reduction in isoelectric point, with no apparent change in molecular weight. The conversion involves an enzymatic acetylation, and results in an insensitivity to aminopeptidase digestion, suggesting N-terminal blockage. Both the acetylated and unacetylated actins can participate in the assembly of F-actin, but with different efficiencies.
D000107:Acetylation; D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004330:Drosophila / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics
D000199:Actins; D012313:RNA