Enhanced production of virus-inhibiting factor (interferon) in human diploid cells by ultraviolet irradiation and temperature shift-down after stimulation with Newcastle disease virus.
The production of the virus-inhibiting factor or interferon (IF) was highest in cells incubated at 37 C after inoculation with Newcastle disease (ND) virus and decreased as the incubation temperature was lowered. Shift-down of incubation temperature to 32 C or 34 C after incubation at 37 C for 4--7 hr enhanced IF production in cell cultures stimulated with ND virus, as compared with cultures incubated continuously at 37 C. Shift-down to 32 C after incubation at 37 C for 6 hr was optimal for this enhancement of IF yield. Enhanced IF production was also observed in cell cultures irradiated by ultraviolet light 4--7 hr after stimulation with ND virus.
D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007372:Interferons / Q000096:biosynthesis* / Q000494:pharmacology; D007700:Kinetics; D009522:Newcastle disease virus / Q000502:physiology*; D013696:Temperature; D014466:Ultraviolet Rays
D007372:Interferons