Transit patterns of marker compounds given by segmented retrograde intrabiliary injection (SRII) in the isolated in situ perfused rat liver.
The mode of transfer of solutes across the biliary tree epithelium in the reabsorptive direction was studied by the segmented retrograde intrabiliary injection technique in isolated perfused liver of rats. The main purpose was to study the pattern of transit of the radioactive marker compounds through the liver into the venous perfusate. The perfusate was collected as a single pass. All of the marker compounds were quantitatively accounted for in the bile and the perfusate. The apparent volumes of distribution of dextran and sucrose were smaller than that for erythritol. Mannitol was similar to erythritol. The mean transit times were correspondingly: dextran congruent to sucrose < mannitol congruent to erythritol. Dehydrocholate (100 mumol/kg) had no effect on these parameters, but taurocholate (100 mumol/kg) increased the mean transit time for sucrose. This effect was probably related to an increase in biliary tree permeability produced by taurocholate. The present approach appeared to be useful in assessing hepatobiliary function and its alteration by allowing the transit patterns of compounds to be determined in passing from bile through the liver to the blood.
D000818:Animals; D001646:Bile / Q000032:analysis; D001693:Biological Transport, Active; D002250:Carbon Radioisotopes; D003685:Dehydrocholic Acid / Q000494:pharmacology; D003911:Dextrans / Q000378:metabolism; D004896:Erythritol / Q000378:metabolism; D007267:Injections; D008111:Liver Function Tests; D008297:Male; D010477:Perfusion; D051381:Rats; D013395:Sucrose / Q000378:metabolism; D013656:Taurocholic Acid / Q000494:pharmacology; D014316:Tritium
D002250:Carbon Radioisotopes; D003911:Dextrans; D014316:Tritium; D013395:Sucrose; D013656:Taurocholic Acid; D003685:Dehydrocholic Acid; D004896:Erythritol