title: Isolation and structural characterization of the equine erythrocyte receptor for enterotoxigenic Escherichia coli K99 fimbrial adhesin.
abstract: The erythrocyte receptor for Escherichia coli K99 fimbrial adhesin was isolated from equine erythrocytes and characterized as Neu5Gc-alpha(2----3)-Galp-beta(1----4)-GLcp-beta(1----1)-Ceramide. This glycolipid acted as the receptor for K99 by four different experimental approaches: inhibition of equine erythrocyte hemagglutination by preincubation of K99-positive bacteria or purified K99 fimbriae with the isolated glycolipid; inhibition of attachment of K99-positive bacteria to porcine intestinal epithelial cells in the presence of the isolated glycolipid; induction of binding of K99-positive bacteria or purified K99 fimbriae to normally unreactive guinea pig erythrocytes by coating these cells with the isolated glycolipid; and isolation of the receptor by affinity chromatography with K99 coupled to CNBr-activated Sepharose 4B, indicating a strong interaction between K99 and the isolated glycolipid.
mesh_terms: D018830:Adhesins, Escherichia coli; D000268:Adhesiveness; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002240:Carbohydrate Sequence; D004910:Erythrocyte Membrane / Q000032:analysis*; D004926:Escherichia coli / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000276:immunology* / Q000378:metabolism; D006017:Glycolipids / Q000302:isolation & purification; D006162:Guanylate Cyclase; D006168:Guinea Pigs; D006384:Hemagglutination; D006736:Horses / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D011971:Receptors, Immunologic / Q000302:isolation & purification* / Q000378:metabolism; D018000:Receptors, Peptide
chemical_list: D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D006017:Glycolipids; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled