Induction of anamnestic T cell proliferation by antigen-pulsed, bone marrow-derived macrophages.
Bone marrow-derived macrophages (BMM phi) were grown in a liquid culture system in the presence of L cell-conditioned medium as a source of colony-stimulating factor. After a 4-h pulse with antigen, cultured irradiated BMM phi were capable of presenting the antigen to primed T cells as assessed in a T cell proliferation assay. Proliferation was optimal when BMM phi were used between days 5 and 8 of bone marrow cell culture. T cells of Lyt1 and Lyt123 phenotype had to be present at the start of the culture period to yield an optimal response. Conventional antisera and monoclonal antibodies directed against the H-2 I region and the I-A subregion, respectively, proved inhibitory in this system. Cultured BMM phi from low-responder strains failed to present antigens under immune response gene control in a form that was immunogenic to T lymphocytes. Cultured BMM phi might thus serve as a source of antigen-presenting cells in the study of cell-cell interaction and immune response gene regulatory mechanisms.
D000818:Animals; D000906:Antibodies; D000941:Antigens; D000954:Antigens, Surface; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005260:Female; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology*
D000906:Antibodies; D000941:Antigens; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II