Niagara sky blue 6B--a new stain for granulocytic cells.
Using an acidified solution of Niagara sky blue 6B, granules in mature granulocytic cells from peripheral blood and bone marrow stained bright blue to blue-green. Granules in immature granulocytes like myelocytes and promyelocytes stained purple. In leukemic myeloblasts and leukemic monocytes, granular-appearing structures stained purple. In leukemic lymphoblasts, purple granular structures were not visualized. As such, Niagara sky blue 6B can be used to identify granulocytic cells at all maturational stages.
D001391:Azo Compounds; D001853:Bone Marrow / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D006098:Granulocytes / Q000648:ultrastructure*; D006801:Humans; D007938:Leukemia / Q000648:ultrastructure; D009504:Neutrophils / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods*; D014343:Trypan Blue
D001391:Azo Compounds; C009000:pontamine sky blue; D014343:Trypan Blue