title: Evaluation of the action of cholinergic agonists and cyclic guanosine monophosphate (c'GMP) on histamine release from purified rat mast cells.
abstract: Results from previous studies providing conflicting data regarding the effects of cholinomimetic drugs and cyclic 3',5'-guanosine monophosphate salts (cyclic GMP) upon the release of histamine from various tissues. In the present investigations, highly purified rat peritoneal mast cells were incubated with a wide range of concentrations of carbachol, acetylcholine, and cyclic GMP in the absence and presence of the histamine releasing agents compound 48/80 and concanavalin A (Con A). Neither carbachol nor acetylcholine (10(-13) to 10(-4) M) alone caused rat mast cells to secrete histamine. Furthermore, when these drugs were combined with Con A (Con A 1 and 10 microgram/ml, carbachol 10(-13) to 10(-4) M) and compound 48/80 (compound 48/80 0.2 microgram/ml) acetylcholine 10(-13) to 10(-4) M), no enhancement of histamine release was observed. Cyclic GMP (8-bromo and dibutyryl salts) failed to elicit histamine release from rat mast cells over the concentration range 10(-7) to 10(-3) M. As well, cyclic GMP did not enhance Con A (10 microgram/ml)-induced histamine release. In fact this substance inhibited the release by as much as 15% at 10(-3) M concentrations. The present data suggest that neither cholinomimetic drugs nor cyclic GMP evoked direct release or enhance the release of histamine from purified rat peritoneal mast cells.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000378:metabolism; D010277:Parasympathomimetics / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010277:Parasympathomimetics; D003208:Concanavalin A; D002217:Carbachol; D006152:Cyclic GMP; D000109:Acetylcholine