Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein.
A monoclonal antibody specific for human alpha-fetoprotein (HAFP) was derived by the hybridoma technique. Spleen cells from mice immunized with pure HAFP were fused with a mouse myeloma cell line (P3x63-Ag-8) and an anti-HAFP secreting hybridoma cell line was cloned in soft agarose. The HAFP specific antibody was shown to be a monoclonal IgG1 subclass with extremely high avidity for HAFP.
D000818:Animals; D000906:Antibodies; D000915:Antibody Affinity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002417:Cattle; D002999:Clone Cells / Q000276:immunology; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007158:Immunologic Techniques; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D000509:alpha-Fetoproteins / Q000276:immunology*
D000906:Antibodies; D000509:alpha-Fetoproteins