Generation and release of platelet-activating factor (PAF) from enriched preparations of rabbit basophils; failure of human basophils to release PAF.
Rabbit mononuclear cells containing up to 20% basophils and uncontaminated by neutrophils release PAF when stimulated with goat antiserum to rabbit IgE. The amount of PAF detected was a function of basophil concentration but decreased on a per cell basis at high basophil or high total cell concentrations. Calcium ionophore A23187, but not protein A, C5a, or FMLP, initiated rabbit basophil degranulation and PAF release. By contrast, extensive studies using a variety of human leukocyte preparations failed to demonstrate the release of significant levels of PAF from human basophils by IgE-dependent or -independent mechanisms. These results suggest that cells other than the peripheral blood basophil (e.g., the neutrophil) may act as the primary site of PAF production in man.
D000818:Animals; D001491:Basophils / Q000378:metabolism* / Q000502:physiology; D001792:Blood Platelets / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology; D003182:Complement C5; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006041:Goats; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000502:physiology; D008297:Male; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000494:pharmacology
D003182:Complement C5; D013205:Staphylococcal Protein A; D000001:Calcimycin