Isolation and characterization of cell-associated nucleases related to streptococcal extracellular deoxyribonuclease D.
A group of at least four distinct nucleases designated DcI through DcIV were isolated from cellular extracts of group A streptococcal strain S43 and shown to be antigenically similar to streptococcal extracellular deoxyribonuclease (DNase) D. These cellular endonucleases degraded single- and double-stranded deoxyribonucleic acid (DNA) as well as ribonucleic acid (RNA) to acid-soluble oligonucleotides. The products of digestion of DNA bore 5'-terminal phosphates, and in partial digests pdX-pdG linkages were most susceptible and pdA-pdX linkages were most resistant to nuclease action. The enzymes had pH optima of 8.0 to 8.5, were inhibited by NaCl, were unaffected by sulfhydryl modifying reagents, and absolutely required a divalent cation. Nucleases DcIII and DcIV were apparently hydrophobic in nature since they required the presence of detergents for migration on nondenaturing polyacrylamide gels. All four nucleases were electrophoretically distinct on such gels, from each other, and from DNase D. Molecular weights of DcI and DcII were similar to that of DNase D, suggesting that the mobility differences of these enzymes at least are reflections of differing net charges. It is suggested that the cellular nucleases represent a group of processing intermediates in the maturation and excretion of DNase D.
D003851:Deoxyribonucleases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D004720:Endonucleases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000939:Epitopes; D005092:Exonucleases / Q000276:immunology; D008970:Molecular Weight; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000276:immunology; D013347:Subcellular Fractions
D000939:Epitopes; D003851:Deoxyribonucleases; D004720:Endonucleases; D005092:Exonucleases