Phorbol ester tumor promoter modulation of alloantigen-specific T lymphocyte responses.
The phorbol diester tumor promoter PMA was studied for its effects on T lymphocyte activation in allogeneic human mixed lymphocyte cultures. Lymphocyte proliferation was significantly enhanced in cultures with PMA at concentrations greater than or equal to 12.5 ng/ml, and interferon-gamma production was synergistically increased in cultures with PMA at concentrations greater than or equal to 5 ng/ml. In contrast, the generation of HLA-specific cytotoxic T lymphocyte activity was completely inhibited in cultures with PMA at concentrations greater than or equal to 5 ng/ml. Fluorescence-activated cell sorter analysis with OKT mouse monoclonal antibodies indicated these PMA effects on lymphocyte activation are associated with an increase in the number of activated OKT4-positive T lymphocytes.
D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D000939:Epitopes; D005434:Flow Cytometry; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D010704:Phorbols / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000939:Epitopes; D006680:HLA Antigens; D007369:Interferon Inducers; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate