Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract.
We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.
D000818:Animals; D002474:Cell-Free System; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics*; D066298:In Vitro Techniques; D012313:RNA / Q000235:genetics*; D012320:RNA Polymerase III / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000235:genetics
D012313:RNA; D012343:RNA, Transfer; D012320:RNA Polymerase III