[Binding activity of purified anticartilage-antiparathyroid antibodies to the cartilage in vitro (author's transl)].
We studied the binding activities especially the binding to the cartilage of the specific antibodies, which were purified from a new therapeutic agent of osteo-arthrosis, Artroglobina using several tissue materials. A purified specific antibodies pre-adsorbed with the pig liver and muscle homogenates lost the binding activity to the pig heart but retained the specific activity to the pig cartilage. The same results were obtained with the tissues from the rat and the hen. It also had the same activity on human cartilage, and the adsorption with the cartilage from either pig or human led the complete loss of the binding activity to the human cartilage. Evidence of the specific activity of these purified specific antibodies to the human cartilage in vitro suggests that further studies are warranted as a similar specific binding reaction of Artroglobina to the cartilage, in situ may occur of patients with osteo-arthritis and who are being treated clinically.
D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D002356:Cartilage / Q000276:immunology*; D002645:Chickens / Q000276:immunology; D006801:Humans; D008297:Male; D010280:Parathyroid Glands / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology; D013552:Swine / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology*
C033161:artroglobina; D005719:gamma-Globulins