The effect of short term incubation in vitro on the RNA content of regenerating mouse epidermis.
The effect of short term incubation in vitro on RNA content in regenerating and normal epidermis has been investigated. Regenerating mouse epidermis was incubated for three hours, either attached to its underlying dermis or by itself, in either buffered sucrose or Roswell Park Memorial Institute culture medium at 26 degrees C or at 37 degrees C. There is a significant loss of RNA when regenerating epidermis is incubated without being attached to its underlying dermis, at either 26 degrees C or 37 degrees C, although there was little loss of DNA, good incorporation of (3H) orotic acid into RNA, as well as good preservation of epidermal histological details. In contrast, when regenerating epidermis was incubated attached to its dermis, little loss of RNA occurred. Similarly, incubating normal epidermis attached to its dermis results in no loss of RNA. These conditions also result in no significant loss of DNA, good incorporation of (3H) orotic acid into RNA, and preservation of epidermal histological details.
D000818:Animals; D004247:DNA / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D009963:Orotic Acid / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012038:Regeneration; D012867:Skin; D013696:Temperature
D009963:Orotic Acid; D012313:RNA; D004247:DNA