Value of quantitative cell count and amylase activity of peritoneal lavage fluid.
From January 1974 through July 1979, 1,588 patients underwent diagnostic peritoneal lavage. The test had an accuracy of 98.6%, sensitivity of 94.3%, and specificity of 99.8%. It was true positive in 21.9%, false positive in 0.1%, false negative in 1.3%, and true negative in 76.6%. Fifty-nine patients from the true-positive group had grossly equivocal tests, but had positive lavage results based on quantitative cell count. Thus without cell count the test would have a sensitivity of 78.3%, accuracy of 94.8%, and specificity of 99.8. Eight patients had positive lavage based on WBC count but negative RBC count; all of these patients had bowel injuries. Measurement of lavage fluid amylase resulted in minimal or no improvement in the accuracy (0.06%), sensitivity (0.3%), or specificity (0.0%). Five of six patients with positive amylase levels but grossly negative tests had concomitant positive WBC count. The added cost of the amylase measurement is estimated to be $154,472. Peritoneal lavage has high accuracy, sensitivity, and specificity. Cell counts significantly improve sensitivity. Patients with a grossly equivocal test but with a positive cell count should undergo laparotomy. The lavage-fluid amylase measurement is costly and is of insignificantly yield.
D000007:Abdominal Injuries / Q000175:diagnosis* / Q000201:enzymology; D000681:Amylases / Q000032:analysis*; D001202:Ascitic Fluid / Q000166:cytology* / Q000201:enzymology; D004796:Clinical Enzyme Tests; D004906:Erythrocyte Count; D006801:Humans; D007958:Leukocyte Count; D007507:Therapeutic Irrigation; D014949:Wounds, Nonpenetrating / Q000175:diagnosis
D000681:Amylases