The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes.
A library of cloned cDNAs representative of lymphocyte total poly(A)+ RNA was screened with total DNA probes at high clone density. 10% of the recombinants showed the presence of sequences which are repeated in the genome. Further analysis of six such isolated cDNA clones indicated that they contain different families of repetitive sequences with reiteration frequencies of between 150 and 45,000 copies per haploid genomes. Five of the six clones were found to contain single copy sequences as well as a repetitive sequence. cDNA clones containing repetitive sequences have been found to be derived from high, intermediate and low abundance classes of lymphocyte poly(A)+ RNA.
D003001:Cloning, Molecular; D004261:DNA Replication; D004274:DNA, Recombinant; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger
D004274:DNA, Recombinant; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA