Spatially distinct allodeterminants of the H-2Kk molecule as detected by monoclonal anti-H-2 antibodies.
In an attempt to delineate spatial relationships between various allodeterminants of cell surface MHC antigens, competitive binding studies were performed using 4 different monoclonal antibodies, each of which reacted with the H-2Kk antigen but with different serologic specificities. Competition was studied by examining the effect of unlabeled antibodies on the binding of each 125I-labeled antibody to spleen cells of the H-2a haplotype. Mutual inhibition was observed between 2 of the antibodies, and a 3rd antibody of lower affinity was inhibited by the first 2 antibodies but did not itself inhibit the binding of these antibodies. The 4th antibody did not block the binding of the other 3 labeled antibodies, and binding of this 4th labeled antibody was only partially inhibitable by the other 3 antibodies. These results indicate the presence of at least 2 spatially distinct allodeterminants on H-2Kk molecules expressed on the cell surface.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D011232:Chemical Precipitation; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology; D007518:Isoantibodies; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D013154:Spleen / Q000276:immunology
D000906:Antibodies; D000954:Antigens, Surface; D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera; D007518:Isoantibodies