title: Insulin stimulates the incorporation of 32Pi into ribonucleic acid in cultured sertoli cells.
abstract: Chronic treatment of cultured Sertoli cells with insulin, FSH, or testosterone does not affect the number of attached cells or the amount of total RNA or poly(A)+ RNA per culture dish. However, Sertoli cells cultured in the presence of insulin have a nearly 2-fold stimulated incorporation of 32Pi into poly(A)+ RNA. The other hormone treatments did not induce any stimulation over control values. The t1/2 of the total poly(A)+ RNA was determined in pulse-chase experiments. None of the hormone treatments affected the t1/2 (16 h) of the total poly(A)+ RNA. The incorporation of [3H]uridine and 32Pi into the total nucleotide pools and into some individual nucleotides was determined by high pressure liquid chromatography analysis of acid-soluble extracts. Insulin-treated cells had approximately a 1.5-fold increased specific activity of [3H]uridine or 32Pi into the total nucleotide pool and in ATP, GTP, and UTP pools. Insulin apparently stimulates the uptake of phosphorylation (or both) of free nucleosides.
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology; D013997:Time Factors
chemical_list: D007328:Insulin; D010710:Phosphates; D012333:RNA, Messenger; D011061:Poly A; D013739:Testosterone; D012313:RNA; D005640:Follicle Stimulating Hormone