title: 'Acrylamide-induced' neuropathy and impairment of axonal transport of proteins. I. Multifocal retention of fast transported proteins at the periphery of axons as revealed by light microscope radioautography.
abstract: The axonal transport of proteins was studied in ciliary ganglia of control and acrylamide-treated chickens. After an intracerebral injection of [3H]lysine, the distribution of axonally transported proteins was analyzed in the preterminal segments of preganglionic axons and in their caliciform nerve endings by means of quantitative light microscope radioautography. By 7 days after the injection of [3H]lysine, the concentration of labeled proteins in the preganglionic axons was either similar or slightly increased in acrylamide-treated chickens as compared to controls. In contrast, by 3 h, whereas the axons of controls were poorly radioactive, 20-30% of the axons in acrylamide-treated chickens displayed focal and intense labeling at their periphery; simultaneously, in acrylamide treated chickens, 20-30% of the nerve endings contained a decreased amount of radioactive proteins. It is concluded that acrylamide induces multifocal retention of fast axonally transported proteins in preterminal segments of certain axons.
mesh_terms: D000178:Acrylamides / Q000633:toxicity*; D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000187:drug effects; D001345:Autoradiography; D001370:Axonal Transport / Q000187:drug effects*; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005726:Ganglia, Parasympathetic / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009802:Oculomotor Nerve / Q000187:drug effects
chemical_list: D000178:Acrylamides; D009419:Nerve Tissue Proteins