Membrane surface properties of normal and malignant cells: partition in aqueous two-polymer phase systems.
The partition of normal and malignantly transformed fibroblast lines and cell lines initiated from malignant human astrocytomas and a benign ganglioneuroma has been examined in aqueous dextran-polyethylene glycol phase system containing phosphate buffer with a low phosphate/sodium chloride ratio. The malignant astrocytomas showed a significantly lower partition coefficient as compared with the benign ganglioneuroma. Treatment of astrocytoma cells with dexamethasone caused an increase in the partitioning of the cell population. No differences were found in the partition behaviour of normal BHK-21 cells and their malignant transformants, the TRES fibrosarcoma cells. Polyoma and simian virus-transformed 3T3 fibroblasts showed partition ratios similar to the untransformed cells. Dexamethasone pre-treatment had no effect on the partition behaviour of these cells. The significance of these observations has been discussed in relation to the surface hydrophobicity and the neoplastic state.
D000818:Animals; D001254:Astrocytoma / Q000503:physiopathology*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003907:Dexamethasone / Q000494:pharmacology; D003911:Dextrans; D005347:Fibroblasts / Q000502:physiology*; D005354:Fibrosarcoma / Q000503:physiopathology*; D005729:Ganglioneuroma / Q000503:physiopathology*; D006801:Humans; D051379:Mice; D011092:Polyethylene Glycols; D013499:Surface Properties
D003911:Dextrans; D011092:Polyethylene Glycols; D003907:Dexamethasone