[In vitro synthesis of tyrosine hydroxylase].
RNA from PC 12 cells was translated in a cell-free system and the peptides synthesized in vitro were analyzed by immunoprecipitation with an anti-T.H. serum raised in Rabbit. Analysis of the immunoprecipitate on a SDS-polyacrylamide gel reveals a major band with a molecular weight of 62000 dalton. A protein of the same molecular weight was also precipitated from a PC12 cell lysate. This molecular weight of 62000 dalton corresponds to the reported for tyrosine hydroxylase purified from PC12 cells.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D066298:In Vitro Techniques; D008970:Molecular Weight; D012313:RNA / Q000302:isolation & purification / Q000502:physiology; D011817:Rabbits; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis*
D012313:RNA; D014446:Tyrosine 3-Monooxygenase
Synthèse in vitro de la tyrosine hydroxylase.