Tumorigenicity of partial transformation mutants of Rous sarcoma virus.
Chicken embryo cells infected with partial transformation mutants of Rous sarcoma virus were tested for tumor-forming ability in chickens and in nude mice. Cells transformed by each of these partial transformation mutants display different combinations of transformation parameters. They therefore present a potentially favorable system for analyzing which properties of transformed cells are necessary for tumor formation. We found that the relative tumorigenicity of the virus mutants was generally similar in chickens and in nude mice, except that certain temperature-conditional mutants appeared to be sensitive to the differences in body temperature of the two experimental animals. (The body temperature of nude mice is 4 to 5 degrees C lower than that of chickens). Thus, the nude mouse appears to be a suitable system for testing the tumorigenicity of transformed chicken cells. Because mice are nonpermissive for Rous sarcoma virus infection and replication, it was possible to recover the transformed chicken cells from the tumors in this host and to determine what phenotypic changes they had undergone during tumor development. We also examined the relationship between various cellular properties of the virus-infected chicken cells in vitro and their tumorigenicity in nude mice. The combined results of these two studies indicated that anchorage independence and plasminogen activator production were highly correlated with the tumor-forming ability of these cells, whereas loss of fibronectin did not correlate with tumorigenicity. Furthermore, the inability of the least tumorigenic virus mutant to stimulate the phosphorylation of a 36,000-Mr target of pp60src raises the possibility that the 36,000-Mr protein plays a role in tumor formation.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001831:Body Temperature; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D002645:Chickens; D006084:Graft Rejection / Q000528:radiation effects; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D019000:Phosphotyrosine; D001357:Sarcoma, Avian / Q000201:enzymology / Q000382:microbiology*; D013045:Species Specificity; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis; D014774:Virulence
D019000:Phosphotyrosine; D014443:Tyrosine