Generation of T cell colonies from responder strain 2 guinea pigs that recognize the copolymer L-glutamic acid, L-lysine in association with nonresponder strain 13 Ia antigens.
T cells from nonimmune responder strain 2 guinea pigs were primed in vitro to the copolymer GL in association with allogeneic, nonresponder strain 13 PEC. T cells that recognized GL in association with strain 13 Ia were separated from alloreactive T cells by cloning the population in soft agar following the priming in liquid culture. The existence of T cells of responder origin that recognize antigen in association with nonresponder macrophages is most consistent with clonal deletion models of Ir gene function.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D010455:Peptides / Q000494:pharmacology*; D011099:Polyglutamic Acid / Q000494:pharmacology* / Q000031:analogs & derivatives; D011107:Polylysine / Q000494:pharmacology* / Q000031:analogs & derivatives; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010455:Peptides; D011107:Polylysine; D011099:Polyglutamic Acid; C030391:poly(glutamic acid-lysine)