title: Rapid demonstration of Legionella pneumophila in unembedded tissue. An adaptation of the Giménez stain.
abstract: The Giménez stain, originally developed for demonstrating rickettsiae, readily stained the Legionnaires' disease bacterium (Legionella pneumophila) in frozen tissue sections and smears of fresh or formalin-fixed lung tissue from patients who had confirmed Legionnaires' disease. With the Giménez procedure, the bacterium stained bright red against a blue-green background. The tissue Gram procedures also stained L. pneumophila in frozen sections and smears, but the staining reaction was weak, and these stains were neither as sensitive nor a consistent as the Giménez procedure.
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D005629:Frozen Sections; D006801:Humans; D007877:Legionnaires' Disease / Q000382:microbiology*; D008168:Lung / Q000382:microbiology; D013194:Staining and Labeling