Inhomogeneities in glycoprotein cytochemistry of secretory granules in rat-parotid acinar cells after selective beta 1-adrenoceptor stimulation.
The distribution of periodate-positive glycoproteins was studied in parotid acinar cells of the rat after stimulation with the specific beta 1-adrenoceptor agonist prenalterol. After two weeks of daily injections, the majority of secretory granules were larger, less electron dense, and often exhibited a bipartite or pleomorphic PA-CrA-silver staining pattern. A great variation in the staining pattern of the secretory granules was seen among different cells but, within any individual cell, most granules displayed a similar pattern. The staining reaction suggests a mucoid transformation of the granules, or a reorganization of the glycoproteins within the granules. The variation among cells in the staining pattern of granules may suggest that there are different populations of acinar cells or of secretory granules.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D003594:Cytoplasmic Granules / Q000032:analysis / Q000187:drug effects* / Q000648:ultrastructure; D004792:Enzyme Precursors; D006023:Glycoproteins; D006651:Histocytochemistry; D007545:Isoproterenol / Q000494:pharmacology; D008854:Microscopy, Electron; D010306:Parotid Gland / Q000648:ultrastructure*; D011217:Practolol / Q000031:analogs & derivatives / Q000494:pharmacology; D011294:Prenalterol; D051381:Rats; D011919:Rats, Inbred Strains
D000318:Adrenergic beta-Agonists; D004792:Enzyme Precursors; D006023:Glycoproteins; D007545:Isoproterenol; D011294:Prenalterol; D011217:Practolol