Effect of 5-aza-2'-deoxycytidine on antibody formation and DNA synthesis in rat spleen.
5-Aza-2'-deoxycytidine which is preferentially taken up by the lymphatic tissue and is incorporated into DNA strongly affects the ability of the immune system to synthesize IgG antibodies against sheep red blood cells without affecting IgM antibody formation. A single dose of the drug results in a prolonged inhibition persisting 14 days after the secondary immunization. The inhibitory effect is dose-dependent and is maximal when 5-aza-2'-deoxycytidine is given 2 days after sheep erythrocytes. The drug affects the utilization of thymidine for the synthesis of DNA in the spleen; under certain conditions the enhancement of the rate of DNA synthesis in the spleen has been observed.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D000077209:Decitabine; D005260:Female; D006386:Hemagglutination Tests; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D000077209:Decitabine; D004247:DNA; D001374:Azacitidine