Sectioning and imaging hard mineral fibres in biological tissues.
A technique is described which overcomes the problems associated with sectioning biological tissue containing hard mineral fibres. 0.2--0.5 micrometer thick sections were cut with a diamond knife, placed in a folding grid, conventionally stained with uranyl acetate and lead citrate and viewed at an accelerating voltage of 200 kV in the scanning transmission mode.
D000818:Animals; D001194:Asbestos / Q000032:analysis; D017639:Asbestos, Amosite; D017638:Asbestos, Crocidolite; D006652:Histological Techniques; D008168:Lung / Q000032:analysis* / Q000648:ultrastructure; D008264:Macrophages / Q000032:analysis* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008903:Minerals / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling
D008903:Minerals; D017638:Asbestos, Crocidolite; D017639:Asbestos, Amosite; D001194:Asbestos