title: alpha 1-Microglobulin from normal and pathological urines.
abstract: alpha 1-Microglobulin was purified from normal and pathological urines. Significant differences were found in the amino acid compositions of the alpha 1-microglobulin isolated from these two sources. In addition electrofocusing of alpha 1-microglobulin from normal urine gave rise to two peaks of equal intensity with rather acidic isoelectric points (3.8 and 4.2), whilst alpha 1-microglobulin from pathological urine showed two peaks in a 1:5 ratio with less acidic isoelectric points (4.2 and 4.7). Further charge heterogeneity was also observed in the second peaks from both sources. The sugar compositions were also established, as well as the N-terminal sequences of the alpha 1-microglobulin of both peaks isolated from normal and pathological urines.
mesh_terms: D000510:Alpha-Globulins / Q000652:urine*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D003554:Cystinosis / Q000652:urine*; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D011863:Radioimmunoassay; D012016:Reference Values
chemical_list: D000510:Alpha-Globulins; D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; C016001:alpha-1-microglobulin