RNA-dependent DNA polymerase activity in ocular granulocytic sarcoma associated to acute myelomonocytic leukemia in Turkish children. Biochemical and immunological characterization of the enzyme.
The present study describes the separation and purification of a reverse transciptiase from an orbital tumor of a patient with acute myelomonocytic leukemia. Specific reaction conditions with respect to ionic requirements and template-primers are reported. The purified enzyme was able to transcribe (rA)n . (dT)12, (rC)n . (dG)12, (OMeC)n . (dg)12 and the 70 S RNA from R(Mu)LV. Serological studies that the reverase transcriptase is antigenically related to reverse transcriptase from the type C woolly monkey virus-gibbon ape leukemia virus group.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002648:Child; D000882:Haplorhini; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000150:complications / Q000201:enzymology*; D009918:Orbital Neoplasms / Q000201:enzymology* / Q000556:secondary; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D012190:Retroviridae / Q000276:immunology; D012842:Sarcoma Virus, Woolly Monkey / Q000276:immunology; D014158:Transcription, Genetic; D014421:Turkey
D000914:Antibodies, Viral; D012194:RNA-Directed DNA Polymerase