title: Antigen-specific mast cell degranulation in contact sensitivity to picryl chloride. An early event.
abstract: Mast cells from the peritoneum of mice painted with the contact sensitizing agent picryl chloride degranulate when exposed to antigen (TNP) in vitro. Degranulation was consistently demonstrated 4 days after painting which associated with the ability of mice to produce contact sensitivity reactions as measured by ear swelling and radiometric assays. Serum reagin or reagin-producing cells could not be detected until 6 days after painting but TNP-phage neutralizing activity was detected after 2 days. Mast cell degranulation could be elicited by TNP or DNP derivatives indicating the involvement of antibody.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D003594:Cytoplasmic Granules; D003877:Dermatitis, Contact / Q000276:immunology* / Q000473:pathology; D004431:Ear, External / Q000473:pathology; D000939:Epitopes; D007114:Immunization; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D010323:Passive Cutaneous Anaphylaxis; D010853:Picryl Chloride / Q000276:immunology*; D011935:Reagins / Q000096:biosynthesis; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000939:Epitopes; D011935:Reagins; D014301:Trinitrobenzenes; D010853:Picryl Chloride