title: [Effect of the extraction methods and drug form components on the determination of gramicidin C in buccal tablets].
abstract: The effect of solvents, extraction procedures and separate components of the troche mass on the results of biological activity determination of gramicidin S with the agar-diffusion method was studied. It was found that irrespective of the solvent and the number of extractions only 76--81 per cent of the antibiotic were determined in the troches. Fruit essence and sugar had a significant effect on the results of gramicidin S biological activity determination as compared to the other components of the dosage form. When accessory substances included in the troches were added to the standard solution, the values of their biological activity were close to the calculated ones.
mesh_terms: D000362:Agar; D001412:Bacillus subtilis / Q000187:drug effects; D004058:Diffusion; D004347:Drug Interactions; D006096:Gramicidin / Q000032:analysis / Q000302:isolation & purification*; D010592:Pharmaceutic Aids / Q000494:pharmacology*; D012996:Solutions; D013607:Tablets
chemical_list: D010592:Pharmaceutic Aids; D012996:Solutions; D013607:Tablets; D006096:Gramicidin; D000362:Agar
vernacular_title: Vliianie metodov ékstraktsii i komponentov lekarstvenoĭ formy na oppredelenie gramitsidina c v zashchechnykh tabletkakh.