title: Radioimmunoassay for human pancreatic secretory trypsin inhibitor: measurement of serum pancreatic secretory trypsin inhibitor in normal subjects and subjects with pancreatic diseases.
abstract: A reliable radioimmunoassay (RIA) for human pancreatic secretory trypsin inhibitor (PSTI) has been developed. The method is highly sensitive (0.4 ng/ml), reproducible and specific. A good parallel relationship was observed between the standard curve and dilution curves for serum and urine. The PSTI bound to trypsin-alpha 2-macroglobulin complexes was found not to be immunoreactive, whereas a part of the psti-trypsin complex was immuno-reactive. In healthy individuals, serum PSTI level ranged from 5.4 ng/ml to 16.0 ng/ml, the average being 11.3 ng/ml (S.D. +/- 2.7). Elevated values were observed in patients with acute pancreatitis (highest value 3200 ng/ml), and in some patients with chronic relapsing pancreatitis.
mesh_terms: D000208:Acute Disease; D000328:Adult; D000818:Animals; D002415:Cats; D002417:Cattle; D002908:Chronic Disease; D004285:Dogs; D006801:Humans; D010189:Pancreatic Juice / Q000032:analysis*; D010195:Pancreatitis / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D012756:Sheep; D014357:Trypsin / Q000097:blood; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000097:blood*; D014361:Trypsin Inhibitors / Q000097:blood*; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D014361:Trypsin Inhibitors; D000511:alpha-Macroglobulins; D014359:Trypsin Inhibitor, Kazal Pancreatic; D014357:Trypsin