Amino acid sequences of transport peptides associated with canine exocrine pancreatic proteins.
Using microsequencing techniques and proteins labeled in vitro with tritiated amino acids we have obtained the following NH2-terminal sequences for six canine pancreatic presecretory proteins: pretrypsinogen 1, pretrypsinogen 2+3, prechymotrypsinogen 2, preproelastase1, preporcarboxypeptidase A1, preamylase. Points of cleavage by the transport peptidase, indicated by the vertical arrows, were located from sequences of authentic products synthesized in the presence of membranes of the rough endoplasmic reticulum. All of the identified residues in the pancreatic transport peptides are hydrophobic. Predictions of secondary structure were calculated for each of the transport peptides. The data indicated neither a common primary of secondary structure which could be interpreted as the signal for functional binding of the nascent presecretory protein to the rough endoplasmic reticulum membrane. These findings suggest that the initial interaction with the membrane or membrane receptor may depend in part, on the hydrophobic nature of the transport peptides. Five of the presecretory proteins showed a region with a high probability of forming a beta-turn immediately following the cleavage point. This feature may give the nascent peptide a region of flexibility that would facilitate both its insertion as a loop structure into the membrane and its cleavage by the transport peptidase. The sequences of authentic secretory products derived from a variety of pancreatic tissues suggest that hydrophilic residues are required immediately following the cleavage point in order to allow translocation of the nascent polypeptide chains across the membrane.
D000595:Amino Acid Sequence; D000681:Amylases / Q000302:isolation & purification; D000818:Animals; D004285:Dogs; D004792:Enzyme Precursors / Q000302:isolation & purification; D051379:Mice; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000302:isolation & purification; D011487:Protein Conformation; D051381:Rats; D013045:Species Specificity
D004792:Enzyme Precursors; D000681:Amylases; D010447:Peptide Hydrolases