The immunosuppressive effect of monoclonal anti-Lyt-1.1 antibodies in vivo.
Monoclonal anti-Lyt-1.1 alloantibody was produced as tissue culture supernatant and administered to mice. The antibody, given intraperitoneally, resulted in the suppression of all T cell functions studied, but was without direct effect on B cells. Thus, skin and tumour allograft survival was prolonged and there was suppression of the delayed-type hypersensitivity response; T cell help inthe anti-sheep red blood cell antibody response, responder cells in the mixed lymphocyte reaction (MLR), leucoagglutinin-responsive cells, cytotoxic T cell (Tc) function and the induction of Tc were either totally or partially suppressed, all these responses being mediated by Lyt-1+2- or Lyt-1+2+ cells in CBA/H mice. By contrast, there was no inhibitory effect on the MLR-stimulating or lipopolysaccharide-responsive cells. The administration of the anti-Lyt-1.1 antibody was accompanied by a depletion of Lyt-1.1+ T cells from both spleen and lymph node. These studies indicate that the monoclonal anti-Lyt-1.1 antibody is active in vivo with a selective effect on T cells. The results also have important implications for studies of T cell interactions in the mouse in vivo, and for similar studies in man.
D000373:Agglutinins / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D000950:Antigens, Ly / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D006968:Hypersensitivity, Delayed; D007166:Immunosuppressive Agents / Q000276:immunology*; D007962:Leukocytes; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D011506:Proteins; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000276:immunology
D000373:Agglutinins; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000939:Epitopes; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D011506:Proteins; C100155:leukoagglutinins, leukocytes