PLC/PRF/5 (Alexander) hepatoma cell line: further characterization and studies of infectivity.
The Alexander hepatoma cell line, PLC/PRF/5, was studied for evidence of hepatitis B virus markers and alpha-fetoprotein. Only hepatitis B surface antigen and alpha-fetoprotein were detected. Induction experiments with 5-iodo-2'-deoxyuridine and inoculation of chimpanzees with whole cells or tissue culture fluid did not reveal evidence of synthesis of additional hepatitis B virus markers or of production of infectious virus.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006511:Hepatitis B Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000382:microbiology*; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000914:Antibodies, Viral; D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D000509:alpha-Fetoproteins