Electronmicroscopical detection of iota-carrageenan as its ruthenium red complex.
Specific detection of iota-Carrageenan (i-CAR) at the ultrastructural level has been obtained by coupling with ruthenium red (RR) - an electron microscopic stain. The i-CAR-RR complex showed electron density on carbon layers. Peritoneal macrophages were treated with the complex and after 3 h it caused the same morphological changes in macrophages as iota-Carrageenan alone. On the surfaces of macrophages, fine filamentous electron dense material - the i-CAR-RR complex - was detected.
D000818:Animals; D002351:Carrageenan / Q000032:analysis*; D002462:Cell Membrane / Q000648:ultrastructure; D008264:Macrophages / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron / Q000379:methods; D012428:Ruthenium; D012430:Ruthenium Red
D012430:Ruthenium Red; D012428:Ruthenium; D002351:Carrageenan