Effects of chromatin protein fractions on transcriptional activity of chicken thrombocyte and erythrocyte chromatin.
1. Chromatin proteins of chicken thrombocytes and erythrocytes were separated into three fractions by successive extraction with 5 M urea containing various salt concentrations and pH values. Molecular composition of protein fractions was determined by SDS-polyacrylamide gel electrophoresis. 2. The efficiences of the chromatin residues after sequential protein extractions as well as those of reconstituted DNA-protein fraction complexes, in serving as a template for the in vitro RNA synthesis were measured in order to identify the effect of each fraction. 3. The different involvement of chromatin protein fractions of template properties of thrombocyte and erythrocyte chromatin was stated.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000502:physiology*; D002645:Chickens; D002843:Chromatin / Q000096:biosynthesis* / Q000097:blood; D004247:DNA / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic
D001798:Blood Proteins; D002843:Chromatin; D012313:RNA; D004247:DNA