beta-D-Glucose 1-phosphate. A structural unit and an immunological determinant of a glycan from streptococcal cell walls.
Glycose 1-phosphate moieties are emerging as important structural units of macromolecular substances imparting special biological functions to these molecules. In the present study, beta-D-glucose 1-phosphate moieties are shown to be structural units and immunological determinants of a bacterial glycan. The glycan is a tetraheteroglycan from the cell wall of Streptococcus faecalis, strain N and is composed of glucose, galactose, rhamnose, N-acetylgalactosamine, and phosphate. Several lines of evidence have been obtained for the presence of beta-D-glucose 1-phosphate units in the glycan, including the liberation of glucose by mild acid hydrolysis, the inhibition of the precipitin reaction by beta-D-glucose 1-phosphate, and the formation of levoglucosan on treatment of the glycan with alkali. Work on the preparation of affinity adsorbents for isolating the new types of antibodies directed at the beta-D-glucose 1-phosphate moieties is in progress.
D000937:Antigen-Antibody Reactions; D002473:Cell Wall / Q000276:immunology; D002849:Chromatography, Gas; D013293:Enterococcus faecalis / Q000276:immunology*; D000939:Epitopes; D005958:Glucosephosphates / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera; D005779:Immunodiffusion; D011135:Polysaccharides, Bacterial; D011233:Precipitin Tests
D000939:Epitopes; D005958:Glucosephosphates; D007106:Immune Sera; D011135:Polysaccharides, Bacterial; C031590:glucose-1-phosphate