Behavior of RNA and protein synthesis during the acclimatization of the carp. Studies with isolated hepatocytes.
1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregated and form tissue. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animals. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.
D000064:Acclimatization; D000818:Animals; D002347:Carps / Q000378:metabolism*; D002449:Cell Aggregation; D003530:Cyprinidae / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012621:Seasons; D014529:Uridine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D012313:RNA; D014633:Valine; D014529:Uridine