Axonal transport of the Ca2+-dependent protein modulator of 3':5'-cyclic-AMP phosphodiesterase in the rabbit visual system.
Water-soluble proteins were extracted from individual retinas, optic nerves, combined optic tracts and lateral geniculate bodies, and superior colliculi of rabbits at 1, 3, and 18 days after injection of [3H]leucine into the right eye. The Ca2+-dependent protein modulator of 3':5'-cyclic-AMP phosphodiesterase (calmodulin) was isolated from these samples by a two-step polyacrylamide gel electrophoresis procedure. An analysis of the radioactivity incorporated into the total soluble proteins and the calmodulin revealed that most of the calmodulin was axonally transported at a slow rate (2--4 mm/day) and represented about 0.45% of the total transported soluble protein.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005829:Geniculate Bodies / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D009900:Optic Nerve / Q000378:metabolism*; D011817:Rabbits; D012160:Retina / Q000378:metabolism*; D013477:Superior Colliculi / Q000378:metabolism
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D007930:Leucine; D002118:Calcium