Restriction mapping of synthetic thyroglobulin structural gene as a means of investigating thyroglobulin structure.
Bovine 33 S thyroglobulin mRNA was reverse transcribed into double-stranded DNA under conditions allowing the synthesis of a complete 8 kilobase pair copy. A physical map of the resulting synthetic thyroglobulin structural gene was constructed using six restriction endonucleases. The following conclusions could be drawn: (i) the polypeptide chains in thyroglobulin subunits are identical; (ii) thyroglobulin is composed of a major class of molecules sharing the same primary structure; (iii) there is no evidence for precise internal repetition in the structure of thyroglobulin subunits.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005796:Genes; D008970:Molecular Weight; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D013954:Thyroglobulin / Q000096:biosynthesis*
D012333:RNA, Messenger; D004247:DNA; D013954:Thyroglobulin; D012194:RNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes