Antiserum against formalin-fixed human milk fat globule glycoprotein for immunohistochemistry of normal and malignant apocrine epithelium.
A rabbit antiserum was raised against formalin-fixed glycoprotein isolated by lectin affinity chromatography from human milk fat globules. After adsorption with erythrocytes and normal blood leukocytes the antiserum detected in immunoblotting of fresh mammary carcinoma tissue a major component of 75 000 dalton apparent molecular weight. The antiserum specifically decorated normal and malignant apocrine epithelium in sections of formalin-fixed tissues. The usefulness of such antisera for routine immunohistochemical diagnosis of metastatic carcinomas is demonstrated.
D000941:Antigens / Q000032:analysis*; D001940:Breast / Q000166:cytology* / Q000473:pathology; D001941:Breast Diseases / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D006177:Gynecomastia / Q000473:pathology; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008895:Milk, Human / Q000032:analysis*; D018396:Mucin-1; D010144:Paget's Disease, Mammary / Q000473:pathology; D011247:Pregnancy
D000941:Antigens; D008565:Membrane Proteins; D018396:Mucin-1