title: Calcium binding sites of synaptosomes from insect nervous system as probed by trivalent terbium ions.
abstract: Terbium binding to synaptosomes from the central nervous system of the locust was studied by fluorescence spectroscopy and electron microscopy. The protein-sensitized fluorescence of terbium was used to characterize the calcium binding sites of synaptosomes. As judged by electron microscopy and x-ray analysis, terbium ions produced electron-dense patches in regular arrays on the outer surface of synaptosomal membranes and induced marked aggregation of synaptic vesicles in isolated terminals.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D005724:Ganglia / Q000378:metabolism; D006110:Grasshoppers; D007700:Kinetics; D008854:Microscopy, Electron; D012430:Ruthenium Red / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D013725:Terbium / Q000378:metabolism*
chemical_list: D013725:Terbium; D012430:Ruthenium Red; D002118:Calcium