title: Human soluble antigens and their use in sperm antibody testing.
abstract: Efforts were made to disrupt and solubilize human sperm cells and to evaluate the product for its content of infertility antibody-related antigens. In the procedure that was developed, a well-washed sperm sample is treated with 0.1 M dithiothreitol for 60 min, followed by trypsin at 0.1 mg/ml for 30 min, and then by soybean trypsin inhibitor. A mixture of DNAses I and II are added. After centrifuging, the resuspended pellet (RP) and the final supernatant (FS) show several degrees of cellular breakdown. Immunological evaluation was done with a strongly positive human serum containing sperm-head antibody. From the inhibition of sperm agglutination, we could conclude that the desired soluble antigen was obtained in the FS fraction.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000302:isolation & purification*; D000939:Epitopes; D005260:Female; D006801:Humans; D007246:Infertility / Q000276:immunology; D008297:Male; D012995:Solubility; D013077:Sperm Head / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000906:Antibodies; D000941:Antigens; D000939:Epitopes