Identification of ribonuclease P activity from chick embryos.
RNAase P (EC 3.1.26.5) activity has been identified in chick embryo thigh tissue on the basis of specific cleavage of Escherichia coli 129 nucleotide tRNATyr precursor and has been partially purified by the procedure used for human tissue culture KB cell RNAase P. RNAase P from chick resembles the KB cell RNAase P in substrate specificity, requirement for a divalent cation (Mg2+) and a monovalent cation (K+, Na+ or NH4+) for activity, inhibition by bulk tRNA, ready inactivation by proteases, and increasing instability; with purification. RNAase P activity is also present in whole chick embryos, as well as in liver and heart tissues. Furthermore, crude preparations of RNAase P from chick embryo heart tissue are relatively free of contaminating nucleases.
D000818:Animals; D002642:Chick Embryo / Q000201:enzymology*; D004720:Endonucleases / Q000302:isolation & purification; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006801:Humans; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D012329:RNA, Bacterial / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D043262:Ribonuclease P; D012260:Ribonucleases / Q000302:isolation & purification*; D013379:Substrate Specificity
D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012367:RNA, Viral; D004720:Endonucleases; D012260:Ribonucleases; C468368:RPP14 protein, human; D043262:Ribonuclease P; C470865:ribonuclease P, E coli