title: Immunological relationships between rabbit acrosin and pancreatic trypsin.
abstract: Specific anit-rabbit acrosin antibodies were prepared by immunizing female guinea pigs with rabbit acrosin. Acrosin was purified by molecular sieve and proflavin-Sepharose affinity chromatography. Inhibition studies to characterize unique antigenic determinants of acrosin compared to pancreatic trypsin indicate that anti-rabbit acrosin gamma-globulins specifically reduced the catalytic effects of acrosin but not trypsin on Azocoll. Anti-acrosin antibodies had no effect on altering the catalytic activities of either acrosin or trypsin on the substrate N-alpha-benzoyl-L-arginine ethyl ester.
mesh_terms: D000176:Acrosin / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D001120:Arginine / Q000031:analogs & derivatives; D010450:Endopeptidases / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D007120:Immunochemistry; D010179:Pancreas / Q000201:enzymology*; D011817:Rabbits; D014357:Trypsin / Q000276:immunology*
chemical_list: D000939:Epitopes; D001120:Arginine; C009727:benzoylarginine ethyl ester; D010450:Endopeptidases; D000176:Acrosin; D014357:Trypsin