Interferon-mediated protein kinase in mouse cells treated with iododeoxyuridine (IdUrd) and induced to express endogenous retroviruses.
Treatment of K/Balb cells with IdUrd leads to the expression of endogenous virus and interferon is formed which subsequently can suppress the release of virus into the medium. Under these conditions, a protein kinase activity capable of phosphorylating an endogenous polypeptide of apparent mol. wt. 67000 is detected. This kinase activity is analogous to that induced in K/Balb cells treated with exogenous interferon. The induction of the protein kinase activity in IdUrd-treated K/Balb cells can be blocked by anti-interferon serum, thus providing evidence for the presence and action of interferon.
D015088:2',5'-Oligoadenylate Synthetase; D000818:Animals; D002460:Cell Line; D004790:Enzyme Induction; D007065:Idoxuridine / Q000494:pharmacology*; D007372:Interferons / Q000502:physiology*; D051379:Mice; D010766:Phosphorylation; D011118:Polynucleotide Ligases / Q000378:metabolism; D011494:Protein Kinases / Q000096:biosynthesis*; D011506:Proteins / Q000378:metabolism; D012190:Retroviridae / Q000254:growth & development*; D014775:Virus Activation
D011506:Proteins; D007372:Interferons; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase; D011118:Polynucleotide Ligases; D007065:Idoxuridine