Detection of a new type of mouse eosinophil colony by Luxol-fast-blue staining.
Staining with Luxol-fast-blue was shown to be a satisfactory method for identifying mouse eosinophils. IN agar cultures of mouse marrow stimulated by pokeweed mitogen-stimulated spleen conditioned medium (SCM), 2-10 compact Luxol-fast-blue positive eosinophil colonies varied according to the batch of human plasma used in the culture medium. Similar colony-forming cells were detected in the spleen and peripheral blood but not in the thymus or lymph nodes. Some Luxol-fast-blue positive eosinophil colonies were stimulated to develop by crude mouse lung conditioned medium and by high concentrations of GM-CSF purified from this source. The cells forming Luxol-fast-blue positive eosinophil colonies sedimented more rapidly (5.5-6.5 mm/h) than the cells forming dispersed-eosinophil colonies 94.5 mm/h). Transfer studies using intact colonies or redispersed colony cells failed to demonstrate an interrelationship between the two types of eosinophil colonies and the cells forming Luxol-fast-blue positive eosinophil colonies appear to be a distinct subset of eosinophil percursors.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D004804:Eosinophils / Q000166:cytology* / Q000254:growth & development; D006098:Granulocytes; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007211:Indoles; D008264:Macrophages; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013194:Staining and Labeling
D004396:Coloring Agents; D003470:Culture Media; D007211:Indoles; C018588:Luxol Fast Blue MBS