RNA metabolism in isolated nuclei: effect of temperature on RNA transport from intact and membrane-denuded nuclei.
The kinetics of RNA transport from intact (both inner and outer nuclear membranes present) and membrane-denuded myeloma nuclei were monitored at temperatures between 10 and 37 degrees C. A linear rate for RNA transport was calculated and the log of RNA transported from membrane-denuded nuclei was greater than that transported from intact nuclei and ii) RNA transport from both nuclear preparations exhibited straight line Arrhenius plots. We conclude the nuclear envelope (or a nuclear matrix element) modulates the amount of RNA transported from nuclei and that nuclear membrane thermal phase transitions do not alter the apparent energy of activation for the transport process.
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D007700:Kinetics; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D013696:Temperature
D012313:RNA