Critical parameters in the microcarrier culture of animal cells.
The successful culture of over 60 different cell types on Cytodex TM1 microcarriers has enabled identification of parameters critical for obtaining high cell yields from microcarrier cultures. Careful control of the initial phase of microcarrier culture was found to be critical. Increasing cell density, reducing culture volume and reducing stirring rate during the initial phase all assisted in improving cell yields. Control of pH, inoculum condition, nutrient supply and serum quality, and elimination of mycoplasma were all vital for high cell yields. Knowledge of the in vitro growth properties of the cells made it easier to arrive at optimal conditions for microcarrier culture of each cell type.
D000818:Animals; D001769:Blood; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology*; D003470:Culture Media; D003584:Cytological Techniques; D003637:DEAE-Dextran; D003911:Dextrans; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration
D003470:Culture Media; D003911:Dextrans; D003637:DEAE-Dextran