'Acrylamide-induced' neuropathy and impairment of axonal transport of proteins. II. Abnormal accumulations of smooth endoplasmic reticulum as sites of focal retention of fast transported proteins. Electron microscope radioautographic study.
The distribution of fast axonally transported proteins was studied by electron microscope radioautography in ciliary ganglia of chickens treated or not treated with acrylamide. At 3 h after the intracerebral injection of [3H]lysine, the preganglionic axons of the untreated chickens displayed few silver grains, mainly associated with smooth endoplasmic reticulum (SER) profiles. In most axons of acrylamide-treated chickens, a similar pattern was observed, except in axons which exhibited focal and intense labeling underneath the axolemma: clusters of silver grains indeed overlayed peripheral accumulations of tubulovesicular profiles of SER, dense core vesicles and mitochondria. After impregnation with heavy metals, electron microscope observation of 1 micrometer thick sections showed a locally disorganized SER forming a complex network of tubules intermingled with vesicles and mitochondria. Such a local disorganization of the peripheral SER in the distal part of the axons, could be responsible for the focal stasis of fast transported proteins; it seems to be one of the earliest changes detectable in axons damaged by acrylamide treatment.
D000178:Acrylamides / Q000633:toxicity*; D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport / Q000187:drug effects*; D002645:Chickens; D004721:Endoplasmic Reticulum / Q000187:drug effects*; D005726:Ganglia, Parasympathetic / Q000187:drug effects; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism*
D000178:Acrylamides; D009419:Nerve Tissue Proteins