Enzyme-linked immunosorbent assay for quantification of rabies antibodies in human sera.
A double antibody enzyme linked immunosorbent assay (ELISA) was elaborated for detection of rabies antibodies in human sera. The procedure consisted of coating polyvinylchloride plates with rabbit antirabies serum followed by attachment of partially purified fixed virus and human rabies antibodies. The rabies-specific antibodies in human antisera were quantified by means of antihuman peroxidase conjugate. Titration of antisera from human volunteers immunized with the "Fermi" vaccine revealed excellent correlation of the virus neutralization test and ELISA.
D000914:Antibodies, Viral / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011820:Rabies virus / Q000276:immunology*; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D014765:Viral Vaccines