title: Substance P-related peptides in the hypothalamus of amphibia.
abstract: The occurrence of Substance P-(SP)-related peptides in the hypothalamus of three species of Amphibia (newt, clawed, toad, frog) was studied immunohistochemically employing the direct immunofluorescence method or a double-step technique (indirect immunofluorescence followed by the peroxidase-antiperoxidase complex method). SP-like immunopositive fibers are seen throughout the hypothalamus. They are especially abundant in the preoptic area and in the outer zone of the median eminence, suggesting a role of SP-related peptides in the hypothalamo-hypophysial regulation in these animals. Some SP-like neurons are seen in the posterior hypothalamus and in the preoptic area. In the newt, such SP-like immunopositive neurons occur frequently in the preoptic periventricular grey.
mesh_terms: D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000032:analysis*; D007034:Hypothalamus, Posterior / Q000032:analysis; D008297:Male; D009412:Nerve Fibers / Q000032:analysis; D011301:Preoptic Area / Q000032:analysis; D011893:Rana esculenta / Q000378:metabolism*; D013373:Substance P / Q000032:analysis*; D014319:Triturus / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D013373:Substance P