[Incorporation of uridine into RNA, nucleic acid content and UTP content at functional different lymphocyte subpopulations of the human tonsil (author's transl)].
B- and T-lymphocytes isolated from the human tonsil are separated by means of E-rosette formation in preparative scale. The T-lymphocytes are furthers purified by affinity chromatography on anti-human-light-chain-sepharose. The separated cell fractions are characterized by the distribution of immunological surface markers. - The specific radioactivity of RNA, the uptake rate of labelled uridine as well as the DNA- and RNA-content are higher in the proliferation B-cell fraction. In the size of UTP-pool are no differences between the cell fractions detectable. Because both cell populations contain the same amount of the RNA precursor UTP, the higher specific radioactivity of RNA is in proof of an increased proliferation rate at the B-cells.
D001402:B-Lymphocytes / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D006801:Humans; D009696:Nucleic Acids / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology; D012313:RNA / Q000378:metabolism; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism*
D009696:Nucleic Acids; D014500:Uracil Nucleotides; D012313:RNA; D004247:DNA; D014544:Uridine Triphosphate; D014529:Uridine
Uridineinbau in die RNA, Nukleinsäuregehalt und UTP-Gehalt bei funktionell verschiedenen Lymphozytensubpopulationen der menschlichen Tonsille.