Estimation of affinity and quantity of human antigen-specific serum IgG (blocking antibodies).
An assay for estimation of quantity and quality of antigen-specific IgG against two purified antigens from cow hair and dander is described. The substance concentration of antigen-specific IgG was more than 10 times higher in serum from immunized patients than in serum from non-immunized patients. Almost the same KA values of 5 x 10(9) l/mol were calculated for IgG in sera from immunized and non-immunized patients with allergic asthma to cow hair and dander.
D000485:Allergens / Q000276:immunology; D000818:Animals; D000906:Antibodies; D000915:Antibody Affinity; D001249:Asthma / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D004485:Eczema / Q000276:immunology; D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits; D012220:Rhinitis / Q000276:immunology; D013997:Time Factors
D000485:Allergens; D000906:Antibodies; D000939:Epitopes; D007074:Immunoglobulin G