Large globin RNA molecules and their processing.
RNA containing beta-globin message sequences larger than 2000 nucleotides could be detected in nuclei of murine erythroid cells using cloned beta-globin cDNA. Under steady-state conditions, when nuclear RNA was separated on denaturing agarose gels and covalently bound to diazobenzyloxymethyl-paper, a 4200-nucleotide and a approximately equal to 3500-nucleotide band could be seen. The presence of these large molecules could also be visualized under the electron microscope after hybridization to a beta-globin genomic DNA fragment. We suggest that these molecules are precursors to mature mRNAs. In addition to these large molecules, a series of molecules smaller than 2000 nucleotides were seen. These are postulated to be processing intermediates in the maturation of beta-globin mRNA.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002999:Clone Cells; D005914:Globins / Q000235:genetics*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*
D012313:RNA; D005914:Globins