title: Preservation of nasopharyngeal smears for fluorescent antibody detection of Bordetella pertussis.
abstract: Smears from throat and nasal washings seeded with Bordetella pertussis were either treated with aprotinin (a protease inhibitor) or fixed with 1 or 10% Formalin. These smears were stored at 23, 4, and -70 degrees C. Smears were removed and stained with a fluorescent antibody conjugate for B. pertussis at intervals during 22 days of storage. Results indicate that treatment of smears with 4 or 8 U of aprotinin per ml preserved fluorescent antibody staining qualities of B. pertussis for 22 days; fixation with either concentration of Formalin was unsatisfactory.
mesh_terms: D007611:Aprotinin / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000187:drug effects / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000494:pharmacology; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D011309:Preservation, Biological / Q000379:methods*
chemical_list: D005557:Formaldehyde; D007611:Aprotinin