title: Translation of mRNA for human lymphotoxin in microinjected Xenopus oocytes.
abstract: Synthesis and secretion of biologically active human lymphotoxin (LT) can be detected in Xenopus laevis oocytes following their inoculation with poly(A+) RNA from human stimulated peripheral blood lymphocytes, but not in oocytes inoculated with RNA from unstimulated lymphocytes or from fibroblastoid cells. In size-fractionating mRNA of stimulated lymphocytes most LT activity is found to be coded for by RNA with an approximate sedimentation value of 19 S.
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008233:Lymphotoxin-alpha / Q000096:biosynthesis* / Q000494:pharmacology; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011061:Poly A / Q000008:administration & dosage / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000008:administration & dosage / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D008233:Lymphotoxin-alpha; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D011061:Poly A