Functional association of idiotypic and I-J determinants on the antigen receptor of suppressor T cells.
The serological characteristics of the antigen receptor on 4-hydroxy-3-nitrophenylacetyl (NP) specific suppressor T cell hybridomas were analyzed. Three T-cell hybrids could be lysed with anti-idiotype and complement. The reactivity pattern observed from a panel of anti-idiotypic reagents indicated that NPb determinants were detected on all three hybrid lines. NP conjugates with bovine serum albumin or caproic acid specifically inhibited the complement-mediated lysis of these cells by both anti-NPb idiotype and anti-I-J antisera. These hapten conjugates failed to block lysis by anti-Thy 1 or anti-H-2K antisera on the same target cell populations. The data indicate that both I-J and Igh variable region gene products are intimately involved in the recognition of antigen by suppressor T cells. Finally, the suppressor cell hybrids produce soluble factors that mediate antigen-specific suppression. The characteristics of the cells and their factors indicate that the hybrids correspond to the Tsi or first-order suppressor cells in the suppressor cell pathway.
D000818:Animals; D002460:Cell Line; D000939:Epitopes; D006241:Haptens; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007518:Isoantibodies; D008285:Major Histocompatibility Complex; D051379:Mice; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000939:Epitopes; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies; D009596:Nitrophenols; D010648:Phenylacetates; D011948:Receptors, Antigen, T-Cell; C026242:4-hydroxy-5-nitrophenyl acetic acid