Replication of transformation-defective mutants of the Prague strain of Rous sarcoma virus and isolation of a td mutant from duck-adapted PR-RSV-C.
The replication of transformation-defective mutants of the Prague strain of Rous sarcoma virus subgroup C was studied using roller cultures. Under such conditions, 10(5)--10(6) infectous units of virus per 0.2 ml were produced, as revealed in both the reverse transcriptase and 16Q complementation tests. A new td daPR-RSV-C mutant was isolated from duck-adapted PR-RSV-C. This mutant replicated in roller cultures with equal efficiency as the original td PR-RSV-C. It was verified that td daPR-RSV-C does not transform chicken fibroblasts, is not oncogenic for 3-week-old chickens and has subgroup C host-range specificity. Both td mutants replicate in duck cells and reach the same titres.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000302:isolation & purification; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004372:Ducks; D009154:Mutation; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014779:Virus Replication
D012194:RNA-Directed DNA Polymerase