Double simultaneous staining of B and argyrophil cells of the pancreatic islets: a sequential thiosulphation-aldehyde fuchsin and Grimelius silver procedure.
The thiosulphation-aldehyde fuchsin (TAF) method for the insulin-producing B cells can be followed by the Grimelius silver impregnation for the argyrophil cells. This double staining is useful to study, in normal and pathological tissues, the spatial distribution of the two main endocrine cell populations of the pancreatic islets. A treatment with potassium ferrocyanide has been found to enhance the argyrophilia of A cells.
D000818:Animals; D004285:Dogs; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D008739:Methyl Green; D051381:Rats; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*; D013885:Thiosulfates
D012394:Rosaniline Dyes; D013885:Thiosulfates; D012834:Silver; D008739:Methyl Green