title: Quantitative determination of human plasma erythropoietin using embryonic mouse liver erythroblasts.
abstract: An in vitro bioassay for the quantitative detection of erythropoietin (Ep) in human plasma is described. The method is based on the increased 3H-uridine incorporation into 12-day embryonic mouse liver erythroblasts due to Ep. It was found that 11 out of 12 anaemic patients showed high plasma Ep level, while 7 out of 8 patients suffering from polycythaemia revealed low Ep values. In 4 out of 8 patients with chronic renal failure the Ep level was within the normal range, whereas in the remaining 4 it was higher than normal. The relative simplicity of the method and the reproducibility of the results make it useful even in the routine laboratory.
mesh_terms: D000740:Anemia / Q000097:blood; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004622:Embryo, Mammalian; D004900:Erythroblasts / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D004921:Erythropoietin / Q000097:blood*; D006801:Humans; D007501:Iron / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000097:blood; D008099:Liver / Q000378:metabolism; D051379:Mice; D011086:Polycythemia / Q000097:blood; D012313:RNA / Q000096:biosynthesis
chemical_list: D004921:Erythropoietin; D012313:RNA; D007501:Iron