Development of a cell line secreting monoclonal antibody specific for concanavalin A and use of that antibody as an affinity immunosorbent for tissue culture media used to support long-term T cell growth.
A new cell line, 71A7, secreting a monoclonal mouse IgG1 antibody specific for the protein concanavalin A (Con A) has been established. The stable and rapidly growing line has been propagated in vitro for several months without loss of secretion of antibody. Affinity purified antibody has been used as an immunoadsorbent to remove Con A from tissue culture supernatants for long-term T cell maintenance.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000494:pharmacology*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002241:Carbohydrates / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003208:Concanavalin A / Q000276:immunology*; D003429:Cross Reactions; D003470:Culture Media; D000939:Epitopes; D007074:Immunoglobulin G; D007164:Immunosorbents / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000936:Antigen-Antibody Complex; D002241:Carbohydrates; D003470:Culture Media; D000939:Epitopes; D007074:Immunoglobulin G; D007164:Immunosorbents; D003208:Concanavalin A