Distribution of antigenic determinants recognized by three monoclonal antibodies (Q2/70, Q5/6 and Q5/13) on human Ia-like alloantigens and on their subunits.
The distribution of antigenic determinants recognized by the anti-Ia-like antigen monoclonal antibodies (MoAb) Q2/70, Q5/6 and Q5/13 on molecules coded for by the DR locus and by non-DR loci was investigated using a binding assay with 125I-labeled Ia-like antigens isolated from four B lymphoid cell lines. The determinants reacting with the MoAb Q2/70 and Q5/13 are expressed on all DR alloantigens tested and on BR4X7 specificities, while those reacting with the MoAb Q5/6 are not detectable on DRw7 and BR4X7 molecules. None of the monoclonal antibodies reacted with DC1 molecules. The MoAb Q5/6 and Q5/13 reacted with the isolated beta subunit of the Ia-like antigenic complex, while the MoAb Q2/70 did not react with the isolated chains.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D002874:Chromosome Mapping; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005815:Genetic Code; D006041:Goats; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D011817:Rabbits
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera