The action of Nupercaine on calcium efflux from rat liver mitochondria.
1. Nupercaine inhibits the Ca2+ efflux from rat liver mitochondria observed in the presence of Ruthenium Red, 50% inhibition being obtained at 80 microM-Nupercaine. 2. Neither the Ruthenium Red-stimulated efflux nor its inhibition by Nupercaine can be directly attributed to effects on mitochondrial stability. 3. Nupercaine perturbs the steady-state external Ca2+ concentration in the absence of Ruthenium Red to an extent that is explicable in terms of the inhibition of Ca2+ efflux. 4. Various factors that are likely to be involved in determining steady-state extra-mitochondrial Ca2+ concentrations are discussed.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D003864:Depression, Chemical; D003992:Dibucaine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology
D012430:Ruthenium Red; D003992:Dibucaine; D002118:Calcium