Enhancing the frequency of antigen-specific hybridomas.
Techniques were developed to enhance the frequency of antigen-specific hybridomas by enriching for antibody precursor or-producing lymphocytes prior to cell fusion. Spleen cells of immunized animals were either (a) cultured in vitro for 3-4 days in the presence of the immunizing antigen or (b) adoptively transferred into X-irradiated syngeneic recipients followed immediately by an in vivo antigen boost 4 days prior to use in cell fusion. There was a 10-fold increase in the percentage of wells which develop antigen-specific, antibody-secreting hybridomas following these treatments. These techniques allow an increased yield of hybridomas with a greatly decreased technical effort.
D000818:Animals; D000917:Antibody Formation; D044482:Benzeneacetamides; D002459:Cell Fusion; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D005260:Female; D006822:Hybrid Cells / Q000276:immunology*; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010400:Penicillin G / Q000031:analogs & derivatives / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D044482:Benzeneacetamides; D004136:Dinitrobenzenes; D000939:Epitopes; C015026:benzylpenicilloyl G; D010047:Ovalbumin; D010400:Penicillin G