Dissociation of suction blister roof epidermis with trypsin and desoxyribonuclease into viable single cells.
Human epidermis, obtained in vivo by the suction blister method, was dissociated with trypsin and desoxyribonuclease into a single-cell suspension. Autoradiographic analysis of the blister roof epidermis and of the epidermal cell suspension was performed to show that neither the suction procedure nor the enzymatic dissociation affected DNA synthesis of the epidermal cells.
D000328:Adult; D001345:Autoradiography; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000096:biosynthesis*; D003851:Deoxyribonucleases; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D006801:Humans; D014357:Trypsin
D004247:DNA; D003851:Deoxyribonucleases; D014357:Trypsin