A new method for measuring urinary orotic acid.
A relatively simple and specific method for measuring urinary orotic acid is reported. With this method, orotic acid is enzymatically converted to uridine 5-phosphate. The difference in absorbance produced by a simple colorimetric procedure in the presence and absence of the enzymatic conversion allows specific measurement of orotic acid. Normal values for urine are less than 4 micrograms/mg creatinine in infants and less than 3 micrograms/mg creatinine in older children and adults.
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine; D006801:Humans; D007223:Infant; D009962:Orotate Phosphoribosyltransferase; D009963:Orotic Acid / Q000652:urine*; D010428:Pentosephosphates; D010754:Phosphoribosyl Pyrophosphate; D012016:Reference Values; D013053:Spectrophotometry
D010428:Pentosephosphates; D009963:Orotic Acid; D010754:Phosphoribosyl Pyrophosphate; D003404:Creatinine; D009962:Orotate Phosphoribosyltransferase