[Mutagenesis in Streptomycete cultures exposed to bleomycin].
Mutagenic properties of bleomycin, an antitumor antibiotic were studied with respect to 2 species of streptomycetes producing practically important antibiotics. A multifold increase in the frequency of prototrophic revertants among the survivors of strains His- and Met- of Actinomadura carminata exposed to bleomycin was observed. Bleomycin was effective in induction of various morphological mutants, and auxotrophs at a high survival rate of the spores of Str. cremeus var. tobramycini, a tobramycin-producing organism. It was shown with the method of subsequent mutagenesis that the efficacy of induction of morphological and auxotrophic mutants in germinating spores of Actinomadura carminata, a carminomycin-producing organism by bleomycin in a concentration of 100 micrograms/ml and an exposure time of 5 minutes was much higher that in the latent spores. The mutagenic effect of bleomycin is comparable with that of ionizing radiation.
D000192:Actinomycetales / Q000187:drug effects* / Q000235:genetics; D001761:Bleomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009152:Mutagenicity Tests; D009154:Mutation; D013171:Spores, Bacterial / Q000187:drug effects / Q000235:genetics; D013302:Streptomyces / Q000187:drug effects* / Q000235:genetics; D013997:Time Factors
D001761:Bleomycin
Mutagenez v kul'turakh streptomitsetov pri deĭstvii bleomitsina.