Deglyco-bleomycin. Degradation of DNA and formation of a structurally unique Fe(II) . CO complex.
In analogy with bleomycin, deglyco-bleomycin B2 has been found to form a stable, diamagnetic complex with Fe(II) and CO. Although the stoichiometry of this complex appeared to be the same as that formed with bleomycin, the geometry of the deglyco-bleomycin complex was fundamentally different, especially as regards orientation of the beta-aminoalanine moiety. In the presence of Fe(II) and O2, deglyco-bleomycin A2 and deglyco-bleomycin B2 were found to release [3H]thymine from radiolabeled PM-2 DNA; when employed at limiting concentrations, deglyco-bleomycin A2 and B2 gave about half as much [3H]thymine release as the respective bleomycins. In view of the spectral evidence (Burger, R. M., Horwitz, S. B., Peisach, J., and Wittenberg, J. B. (1979) J. Biol. Chem. 254, 12299-12302) that Fe(II) . bleomycin . CO has the same geometry as the complex formed by initial association of bleomycin, Fe(II), and O2, the accumulated data suggest strongly that all metal complexes of bleomycin (derivatives) capable of DNA degradation need not have the same geometry.
D001761:Bleomycin / Q000031:analogs & derivatives; D002248:Carbon Monoxide; D055598:Chemical Phenomena; D002621:Chemistry; D004279:DNA, Viral; D007501:Iron; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation
D004279:DNA, Viral; D001761:Bleomycin; C033284:deglycobleomycin; D002248:Carbon Monoxide; D007501:Iron