Isolation and partial characterization of the reverse transcriptases of Rauscher murine leukemia virus, simian sarcoma virus and RD-114 virus.
Reverse transcriptases were purified from Rauscher murine leukemia virus, Simian sarcoma virus and RD-114 virus by affinity chromatography using poly(rC)-Sepharose. Molecular weight determinations of the viral enzymes gave values of 80,000 for all three enzyme preparations. Two-dimensional tryptic peptide maps of the purified enzymes showed only a few spots in similar positions.
D000818:Animals; D002415:Cats / Q000382:microbiology; D002846:Chromatography, Affinity; D008970:Molecular Weight; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification*; D011925:Rauscher Virus / Q000201:enzymology*; D012190:Retroviridae / Q000201:enzymology*; D012842:Sarcoma Virus, Woolly Monkey / Q000201:enzymology*; D013045:Species Specificity
D012194:RNA-Directed DNA Polymerase