title: Oxygen transfer from bleomycin-metal complexes.
abstract: Both Fe(III) and Cu(II) complexes of bleomycin (BLM), but not N-acetyl BLM . Fe(III), mediated the transfer of oxygen from iodosobenzene to organic substrates. In analogy with results obtained using certain cytochrome P-450 analogs, cis-stilbene was converted cleanly to the respective oxide, while no more than traces of trans-stilbene oxide were formed from trans-stilbene under identical conditions. The possible relevance of these observations to the degradation of DNA by bleomycin was also studied. In both the presence and absence of O2, BLM . Cu(II) . C6H5IO effected DNA degradation, as judged by the release of [3H]thymine from radiolabeled Escherichia coli DNA. These findings provide a valuable new assay system for the study of bleomycin analogs and suggest the possibility that bleomycin may function as an "oxygen transferase" in its degradation of DNA in situ.
mesh_terms: D001761:Bleomycin; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli; D005296:Ferrous Compounds; D007463:Iodobenzoates / Q000378:metabolism; D007501:Iron; D010100:Oxygen; D013267:Stilbenes / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005296:Ferrous Compounds; D007463:Iodobenzoates; D013267:Stilbenes; D001761:Bleomycin; C008154:2-iodosobenzoic acid; D003300:Copper; D007501:Iron; D010100:Oxygen