A specific stain for sulfhydryl groups in proteins after separation by polyacrylamide gel electrophoresis.
A new method is described for specifically staining protein sulfhydryl groups after the proteins have been separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in slab gels. The stain will detect as little as 0.25 microgram of lysozyme and 1 microgram of most other proteins; the range of sensitivity for a specific protein depending on its sulfhydryl content. Proteins with no cysteine residues (type I collagen) and glycoproteins do not cause spurious staining.
D003545:Cysteine / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D008832:Microchemistry; D008970:Molecular Weight; D009113:Muramidase / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds / Q000032:analysis*
D007202:Indicators and Reagents; D011506:Proteins; D013438:Sulfhydryl Compounds; D009113:Muramidase; D003545:Cysteine