Trypsin digestion in immunoperoxidase staining.
The effect of trypsin digestion on the immunoperoxidase-PAP staining of paraffin sections from normal and abnormal tissue was studied. Trypsin treatment reduced background staining considerably, but enhanced specific staining. Results were, therefore, much easier to read. Possible reasons for this result are discussed.
D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A; D008198:Lymph Nodes / Q000166:cytology; D008223:Lymphoma / Q000473:pathology; D009101:Multiple Myeloma / Q000473:pathology; D010954:Plasmacytoma / Q000473:pathology; D013194:Staining and Labeling; D014357:Trypsin
D007070:Immunoglobulin A; D014357:Trypsin