Enrichment of hapten-specific helper T cells using anti-immunoglobulin combining site antibodies.
Spleen T cells from phosphorylcholine (PC)-primed BALB/c mice were indirectly stained with affinity column-purified heterologous rabbit anti-TEPC 15 (T 15) antibodies. These antibodies were shown to be directed towards antigenic determinants in or near the combining site of T 15 in that their interaction with the myeloma protein was more than 95% inhibitable by PC. The stained cells were sorted into "bright" and "dull" fractions using a fluorescence-activated cell sorter. Sorted populations were then assessed for enrichment and depletion of PC-specific helper activity using an in vivo adoptive transfer assay. The results indicate that the staining procedure employed enabled the specific enrichment of PC helper T cells and therefore suggest that these T cells bear combining site structures similar to that of the myeloma protein T 15.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001666:Binding Sites, Antibody; D002417:Cattle; D002469:Cell Separation; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D006241:Haptens; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology; D010767:Phosphorylcholine / Q000276:immunology; D011817:Rabbits; D011948:Receptors, Antigen, T-Cell; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006241:Haptens; D009194:Myeloma Proteins; D011948:Receptors, Antigen, T-Cell; D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine