title: [Ultrastructural analysis of the effect of antimicrobial drugs on the development of Chlamydia trachomatis in cells cultured in vitro].
abstract: The Authors report the results of an ultrastructural study of the developmental cycle of Chlamydia trachomatis in cell cultures in vitro in the presence of inhibitors of te synthesis of DNA (mitomycin-C), RNA (actinomycin-D and rifampin) and proteins (oxytetracycline and chloramphenicol). The chlamidiae were grown to IUDR-pre-treated McCoy cell cultures and the drugs were added to the medium either immediately after the infection (in the presence of "elementary bodies" only) or after the appearance of the first "initial bodies" (14 hours after infection). The appearance of "initial bodies" or their multiplication was completely prevented in the infected cell cultures in the presence of mitomycin-C, actinomycin-D, rifampin and oxytetracycline added immediately after the infection or 14 hours after the infection, respectively. In the presence of chloramphenicol the appearance of "initial bodies" or their multiplication was possible, but their differentiation into "elementary bodies" was completely inhibited.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002692:Chlamydia trachomatis / Q000254:growth & development / Q000648:ultrastructure*; D002701:Chloramphenicol / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D010118:Oxytetracycline / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D008937:Mitomycins; D003609:Dactinomycin; D012313:RNA; D002701:Chloramphenicol; D004247:DNA; D012293:Rifampin; D010118:Oxytetracycline
vernacular_title: Analisi ultrastrutturale dell'azione di alcuni farmaci antimicrobici sullo sviluppo di Chlamydia trachomatis in colture di cellule in vitro.