Multiple-rate components of axonally transported proteins in the hypothalamo-neurohypophysial system of the rat.
The transport of labeled proteins from the hypothalamus to the neurohypophysis following 35S-methionine injection into the rat supraoptic nucleus was studied using a unique approach adapted for the study of short-axon systems. Multiple-rate components to those found in other neuronal systems were demonstrated. Neurosecretory vesicle-containing proteins (e.g., neurophysins) were transported at fast rates (greater than 120 mm/day), whereas the cytoskeletal protein, actin, moved principally in the slow component of transport. Two-dimensional gel electrophoresis was used to analyze the diverse patterns of labeled proteins found in the various rate components of axonal transport in this system.
D000199:Actins / Q000032:analysis; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D001369:Axons / Q000502:physiology; D003078:Colchicine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009481:Neurophysins / Q000032:analysis; D051381:Rats
D000199:Actins; D009419:Nerve Tissue Proteins; D009481:Neurophysins; D003078:Colchicine