Identification of folate binding protein of mitochondria as dimethylglycine dehydrogenase.
The folate-binding protein of rat liver mitochondria [Zamierowski, M. & Wagner, C. (1977) J. Biol. Chem. 252, 933-938] has been purified to homogeneity by a combination of gel filtration, DEAE-cellulose, and affinity chromatography. This protein was assayed by its ability to bind tetrahydro[3H]folic acid in vitro. the purified protein contains tightly bound flavin and has a molecular weight of about 90,000 as determined by sodium dodecyl sulfate electrophoresis. This protein also displays dimethylglycine dehydrogenase [N,N-dimethylglycine: (acceptor) oxidoreductase (demethylating), EC 1.5.99.2] activity which copurifies with the folate-binding activity. It is suggested that the role of the tetrahydrofolic acid is to accept the formaldehyde produced during the course of the reaction.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D050893:Dimethylglycine Dehydrogenase; D005420:Flavoproteins / Q000378:metabolism; D005492:Folic Acid / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D024101:Mitochondrial Proteins; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D051381:Rats; D012521:Sarcosine / Q000031:analogs & derivatives / Q000378:metabolism; D013057:Spectrum Analysis
D002352:Carrier Proteins; D005420:Flavoproteins; D024101:Mitochondrial Proteins; D005492:Folic Acid; D010089:Oxidoreductases, N-Demethylating; D050893:Dimethylglycine Dehydrogenase; C494400:Dmgdh protein, rat; D012521:Sarcosine