M and N specific hemagglutinins of human erythrocytes stored in glucose solutions.
Alloagglutinins of M and N specificites with unique characteristics found in normal sera are described. Specific M or N agglutination by these sera occurred only when erythrocytes had been exposed to glucose-containing solutions. Conversion to the form of MN recognized by these sera required incubation of the cells in 1 to 2% glucose buffered to physiological or alkaline pH and was dependent on time and temperature of exposure, occurring in hours at 37 C and in days at 4 C. The sera appeared to be detecting an antigenic structure in which interaction between glucose and the amino group of the N-terminal amino acid of the MN receptor has produced a new specificity, analogous to the action of formaldehyde on N receptors and the resulting N-like structure detected by sera from hemodialysis patients.
D000042:Absorption; D001793:Blood Preservation; D002241:Carbohydrates / Q000494:pharmacology; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D005947:Glucose / Q000494:pharmacology*; D006388:Hemagglutinins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000494:pharmacology; D007185:Incubators; D008951:MNSs Blood-Group System; D009439:Neuraminidase / Q000494:pharmacology
D002241:Carbohydrates; D000939:Epitopes; D006388:Hemagglutinins; D007106:Immune Sera; D008951:MNSs Blood-Group System; D009439:Neuraminidase; D005947:Glucose