Isolation and properties of an RNA fraction present in Brucella culture supernatants.
The supernatant fluids of batch and continuous cultures of Brucella strains contained up to 100 mg/l of soluble RNA which could be recovered by precipitation with lysozyme, This RNA fraction had many of the properties of ribosomal RNA and was single-stranded, sensitive to ribonuclease, with an approximate sedimentation constant of 5S, a molecular weight of about 35000 daltons and an adenine; guanine; cytosine; uracil content of 17.5; 26.5; 33; 23 mol% respectively. RNA fractions from lysozyme precipitates evoked high titres of Brucella agglutinins on injection into rabbits and induced acute inflammatory responses in guinea-pig skin. Highly purified RNA fractions prepared by phenol extraction of lysozyme precipitates did not evoke antibodies to Brucella abortus.
D000818:Animals; D000917:Antibody Formation; D002002:Brucella / Q000737:chemistry* / Q000276:immunology; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007122:Immunoelectrophoresis; D012329:RNA, Bacterial / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000276:immunology; D011817:Rabbits
D012329:RNA, Bacterial; D012335:RNA, Ribosomal