title: Rat cerebellar cells in tissue culture. I. An immunological marker for brain vascular endothelial cells (antibodies from the sera of patients with Chagas' disease).
abstract: Immunohistochemical studies showed that immunoglobulins from sera of patients with Chagas' disease bind specifically to endothelial cells in cryostat sections of the rodent brain. In monolayer cultures of 8-day-old rat cerebella both surface and internal labelling of the endothelial cells was detected. The contrast immune sera raised against cold-insoluble globulin (which cross-reacts with fibronectin) also labelled the surface of fibroblasts as well as the endothelial cells. It would appear that Chagasic immunoglobulins are a useful marker for endothelial cells in neural cell culture.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002196:Capillaries / Q000276:immunology; D002531:Cerebellum / Q000098:blood supply* / Q000276:immunology*; D002598:Chagas Cardiomyopathy / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology*; D046508:Culture Techniques; D004727:Endothelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D009474:Neurons / Q000276:immunology; D051381:Rats; D005719:gamma-Globulins / Q000378:metabolism
chemical_list: D000906:Antibodies; D005719:gamma-Globulins