[Synthesis and cloning of DNA, complementary to rabbit globin pre-mRNA].
cDNA synthesized on rabbit bone marrow erythroid cells pre-mRNA was cloned in bacterial plasmids. Cold phenol extracted pre-mRNA was a several times more effective template in the reaction of reverse transcription without oligo(dT) 10-primer than hot phenol extracted pre-mRNA. There was no yield increase of DNA-product on hot phenol extracted pre-mRNA in the reaction of reverse transcription with the oligo(dT)10-primer addition. The "hot phenol" poly (A)+-pre-mRNA was used to obtain the representative, full-sized cDNA. The double-stranded form of this cDNA, obtained with the help of DNA-polymerase I, was inserted into the PstI-site of pBR322 plasmid. About 25% E. coli JC5183 clones, transformed with this hybrid plasmid, were found to contain the globin sequences.
D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005914:Globins / Q000235:genetics*; D009694:Nucleic Acid Precursors / Q000235:genetics*; D010957:Plasmids; D012322:RNA Precursors; D012333:RNA, Messenger / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011817:Rabbits
D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I
Sintez i klonirovani DNA, komplementarnoĭ pre-mRNK globina krolika.