Quantitation of helper factor activity. I. Utilization of the ELISA method to measure helper activity in the secondary antibody response.
We describe here a simple culture system for measuring the effects of regulatory cells or factors on the secondary antibody response which uses the enzyme-linked immunosorbent assay (ELISA) to detect antibody production. This technique offers several advantages over methods which measure hemolytic plaque-forming cells. Helper factors purified from the supernatant of in vitro primed helper cells were shown to specifically enhance antibody production from primed spleen cells by from 2- to 4-fold in this system.
D000818:Animals; D000917:Antibody Formation; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007375:Interleukin-1; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011506:Proteins / Q000378:metabolism*; D013997:Time Factors
D000939:Epitopes; D007375:Interleukin-1; D011506:Proteins; D006433:Hemocyanins