title: Characterization of the phenol soluble and insoluble nonhistone proteins of in vivo (32)P-labelled rat liver nuclei by high-resolution gel electrophoresis.
abstract: The (32)P-labelling patterns of phenol soluble and insoluble nonhistone proteins of in vivo labelled rat liver nuclei freed of the soluble nuclear proteins have been determined after separation by high-resolution gel electrophoresis. The bulk of the proteins of the nuclear residues was phenol soluble. Seven percent of the proteins of the nuclear residues was obtained with the aqueous phase. As shown in this paper both fractions contain (32)P-labelled proteins but they represent different types of nonhistone proteins.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D010636:Phenols; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D012995:Solubility
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D010636:Phenols; D012313:RNA; D004247:DNA