An argyrophil III method for the demonstration of micro- and oligodendroglia.
Micro- and oligodendroglia, plasma and nucleoli of nerve cells, capillary wall and nuclei of astrocytes become visible when sections of formol fixed human brain are immersed, without any previous treatment, into a physical developer of pH 10.5. The staining is inhibited by the catalytic activity of the tissue elements involved. By means of pretreatments with 1% performic acid and 30% sodium rhodanide dissolved in 0.4% sodium hydroxide, the catalytic activity in the unwanted tissue elements is suppressed, and this results in an elective demonstration of micro- and oligodendroglia. Reducing groups of the tissue or any kind of performed nuclei play no role in this silver staining.
D002254:Carbonates; D002467:Cell Nucleus; D002540:Cerebral Cortex / Q000166:cytology / Q000473:pathology; D005561:Formates / Q000494:pharmacology; D006527:Hepatolenticular Degeneration / Q000473:pathology; D006801:Humans; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D012834:Silver; D018030:Silver Compounds; D012972:Sodium Hydroxide / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods*; D013861:Thiocyanates / Q000494:pharmacology
D002254:Carbonates; D005561:Formates; D018030:Silver Compounds; D013861:Thiocyanates; C008817:peroxyformic acid; D012834:Silver; D012972:Sodium Hydroxide; C024553:sodium thiocyanate; C033260:silver carbonate