Relation of age to isoenzyme pattern and total activity of amylase in serum.
Pancreatic and salivary isoenzymes of amylase were determined in serum from 70 subjects. Thin-layer gel/isoelectric focusing was used to separate the isoenzymes. Because other studies (J. Lab. Clin. Med. 90: 141-151, 1977) show that the major isoamylases have isoelectric points between 5.8 and 7.2, we focused the sera on polyacrylamide gel plates with a pH gradient from 5.5 to 8.5. The separated amylase fractions were made visible by direct incubation with a commercially available dye-starch polymer. Isoelectric focusing proved to be convenient, precise, and reproducible, and it can be used as a routine analysis to detect even slight changes in serum amylase distributions. We found that the isoamylase distribution is age dependent, whereas total amylase activity shows no correlation with age.
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000681:Amylases / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood*; D008875:Middle Aged; D010189:Pancreatic Juice / Q000201:enzymology; D010195:Pancreatitis / Q000201:enzymology; D010309:Parotitis / Q000201:enzymology; D012463:Saliva / Q000201:enzymology; D000516:alpha-Amylases / Q000097:blood*
D007527:Isoenzymes; D000681:Amylases; D000516:alpha-Amylases