D-penicillamine induced changes on rat mast cell cyclic GMP levels.
The cGMP accumulation in rat serosal mast cells was measured after cell treatment with D-penicillamine, a sulphydryl containing compound. Preincubating the cells with increasing doses of the drug causes a decrease of basal level of the nucleotide. In cells activated with the degranulating agent 48/80 there is a sharp increase in cGMP intracellular levels. D-Penicillamine prevents the nucleotide accumulation caused by compound 48/80, decreasing even the steady-state level in activated cells. The effect on cGMP metabolism does not correspond to an inhibition of histamine release.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D006152:Cyclic GMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D010396:Penicillamine / Q000494:pharmacology*; D051381:Rats
D010396:Penicillamine; D006152:Cyclic GMP