title: Inhibition of in vitro granulopoiesis by autologous allogeneic human NK cells.
abstract: This study demonstrates the ability of human NK cells to inhibit in vitro granulopoiesis of autologous and allogeneic BM cells. NK lytic activity and GM-CFC inhibition was present among nonstimulated lymphocytes from healthy donors and could be increased by treatment of PBL with IFN. Both the cytotoxic NK cells and the GM-CFC inhibitory cells could be enriched for among nonadherent, low-density cells. High-density cells were not cytotoxic, only inhibitory to a small extent, and could become neither cytotoxic nor more inhibitory after IFN treatment. In contrast, low-density cells showed an increased cytotoxic and GM-CFC inhibitory capacity after IFN treatment. The NK mediated GM-CFC inhibition was dependent on cell contact with BM cells, increased with longer preincubation times, and was most efficient against 7-day GM-CFC as compared with 14 day GM-CFC progenitors. In conclusion, these data provide new information about the human NK cell as a potent inhibitor of in vitro granulopoiesis and also as a possible regulator of hematopoiesis in vivo.
mesh_terms: D001667:Binding, Competitive; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology
chemical_list: D007372:Interferons