Rat liver DNA polymerase-beta: thermal inactivation of RNA- and DNA-dependent activities.
Purified DNA polymerase-beta from rat liver was exposed to thermal inactivation and the remaining activities were then measured either with a hybrid template such as poly(A).(dT)12-18 (R-activity) or with a DNA template such as poly(dA).(dT)12-18 (D-activity). Time course of inhibition of R- and D-activities were identical. Neither activity was protected when the thermal treatment was performed in the presence of the template or dNTPs.
D000818:Animals; D004256:DNA Polymerase I / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D051381:Rats
D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase