title: Kinetics and regulation of cell-free alkaline phosphatase synthesis.
abstract: Regulation of alkaline phosphatase (EC 3.1.3.1) synthesis in a cell-free system from Escherichia coli has been observed. Synthesis from transducing phage deoxyribonucleic acid templates carrying phoA+ occurred in S30 fractions from wild-type or alkaline phosphatase-constitutive mutants. It did not occur in S30) fractions from alkaline phosphatase-negative mutants (phoB). The hybrid gene phoA-lacZ was also subject to phoB control, implying that phoA transcription is regulated. The yield of active alkaline phosphatase per phoA+ gene copy from cell-free synthesis was similar to that of beta-galactosidase. Alkaline phosphatase activity took longer to appear than beta-galactosidase activity. Synthesis of alkaline phosphatase subunits was not delayed, suggesting that a minimum number of subunits are synthesized before formation of active alkaline phosphatase occurs.
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002474:Cell-Free System; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005809:Genes, Regulator; D007700:Kinetics; D009154:Mutation; D010710:Phosphates / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D010710:Phosphates; D012329:RNA, Bacterial; D012333:RNA, Messenger; D000469:Alkaline Phosphatase