Identification of a unique idiotype in cerebrospinal fluid and serum of a patient with multiple sclerosis.
Anti-idiotypic antibodies were prepared in mouse ascites fluid against the CSF-IgG of a patient with multiple sclerosis. After adsorption with pooled human IgG, the ascites fluid antibodies precipitated 20% of labeled autologous CSF IgG. By using a competitive radioimmunoassay, less than one microgram of unlabeled CSF IgG produced 50% inhibition of binding autologous 125I-labeled CSF IgG, whereas 50 micrograms of normal HIgG was not inhibitory. The idiotype could be found in both serum and CSF IgG and persisted over a 5-year period. The absolute concentration of idiotype in the CSF varied somewhat but remained from 4 to 10 times greater than that of the serum. One of 14 heterologous MS CSF was found to contain small amounts of inhibitory protein; eight CSF from patients with other neurologic diseases did not contain the idiotype.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D000939:Epitopes; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007130:Immunoglobulin Idiotypes / Q000134:cerebrospinal fluid*; D007164:Immunosorbents; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology*; D011817:Rabbits; D013997:Time Factors
D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007164:Immunosorbents