[DNA interaction with low molecular ligands of different structure. II. Complexes of DNA with actinomine and its analogs].
DNA-complexes with actinomine and its analogues containing omega-dialkylaminoalkyl groups at 1,9 positions of the phenoxazone moiety were studied by technique of spectrophotometry, viscometry and flow birefringence. In the process of spectrophotometry titration two groups of spectra corresponding to different DNA--ligand ratio in a complex were observed. According to the experimental data the investigated compounds are bounded to DNA by means of intercalation and external binding. There within a region of low degrees of binding the intercalation type of the ligand--DNA interaction prevails. In virtue of the spectrophotometry data the intercalation binding share was calculated. The intrinsic viscosity of a complex increases in the case of ligand intercalation and does not change as it joins to the DNA double-helix from outside. Optical anisotropy of DNA molecule increases linearly irrespective of the way of ligand binding. Data on the flow birefringence permits to conclude that under external binding the angle between the normal to the ligand chromophore plane and the axis of DNA double-helix is about zero. During ligand intercalation the equilibrium rigidity of DNA molecules increases.
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D003609:Dactinomycin / Q000031:analogs & derivatives*; D008024:Ligands; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D008024:Ligands; D003609:Dactinomycin; C010007:actinomine; D004247:DNA
Vzaimnodeĭstvie DNK s nizkomolekuliarnymi ligandami razlichnoĭ struktury. II. Kompleksy DNK s aktinominom i ego analogami.