Immune response to Toxoplasma gondii in man. I. Common idiotypic determinants of toxoplasma-specific human antibodies.
A rabbit antiserum against human anti-toxoplasma antibodies was prepared. The rabbit antiserum was made antiidiotypic by extensive absorption. It bound 70% of radio-labelled autologous antibodies. The same antiidiotypic antiserum bound to varying degrees, three out of four other anti-toxoplasma antibodies isolated from different individuals. Inhibition experiments, using 24 human sera containing varying amounts of antibodies, showed that about 60% of them cross-reacted with the antiidiotypic antiserum, indicating that some common determinants were present on human anti-toxoplasma antibodies. F(ab')2 fragments of antiidiotypic immunoglobulin revealed about 8% idiotype-positive (id+) cells among toxoplasma-induced blasts obtained from toxoplasma-sensitized normal donors.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D011817:Rabbits; D014122:Toxoplasma / Q000276:immunology*
D000906:Antibodies; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007130:Immunoglobulin Idiotypes