Involvement of divalent cations in the complex between the platelet glycoproteins IIb and IIIa.
The major immunoprecipitate (No. 16) seen on crossed immunoelectrophoresis of Triton X-100-solubilized platelet proteins against whole platelet antibodies represents a complex containing the membrane glycoproteins IIb and IIIa. When EDTA is present during the solubilization, immunoprecipitate 16 as such is not observed, and two new arcs, termed 16a and 16b, appear. As with 16 these immunoprecipitates become radioactively labelled on lactoperoxidase-catalyzed iodination of platelets. Immunoprecipitate 16a showed partial immunochemical identity with 16, and was precipitated by an antibody raised against immunoprecipitate 16. The areas covered by immunoprecipitates 16, 16a and 16b were strongly reduced compared to normal with platelets from a patient with Glanzmann's thrombasthenia type II. Such platelets are known to contain reduced amounts of glycoproteins IIb and IIIa. The new arcs appearing when divalent cations are chelated by EDTA thus represent proteins derived from the immunoprecipitate 16 proteins, and divalent cations seem to be necessary to preserve the protein complex containing glycoprotein IIb and IIIa. The different complex formations between the components of immunoprecipitate 16 may reflect biochemical alterations of functional importance.
D001791:Blood Platelet Disorders / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D002413:Cations, Divalent; D011232:Chemical Precipitation; D000939:Epitopes; D006023:Glycoproteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D010980:Platelet Membrane Glycoproteins
D002413:Cations, Divalent; D000939:Epitopes; D006023:Glycoproteins; D010980:Platelet Membrane Glycoproteins