Preparation of Schiff-type dye-SO2 reagents with phosphoric acid and their cytochemical properties.
A new method employing phosphoric acid in place of hydrochloric acid for the preparation of azure A-SO2 and toluidine blue O-SO2 has been described in this communication. It has been found that o-phosphoric acid in a weak concentration can be a suitable substitute for hydrochloric acid in the preparation of these dye-SO2 reagents. These dye-reagents produce excellent blue nuclei in human uterine tumour. Toluidine blue O-SO2 fortified with an amino aicd, glycine also produces excellent blue chromosomes in the squash preparations of grasshopper testes fixed in acetic acid-alcohol. The shelf-life of these two dye-reagents has also been presented herein.
D000818:Animals; D001399:Azure Stains; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure; D005260:Female; D006110:Grasshoppers / Q000648:ultrastructure; D006801:Humans; D008297:Male; D010640:Phenothiazines; D010756:Phosphoric Acids; D013194:Staining and Labeling / Q000379:methods*; D013438:Sulfhydryl Compounds; D013737:Testis / Q000648:ultrastructure; D014048:Tolonium Chloride; D014594:Uterine Neoplasms / Q000648:ultrastructure
D001399:Azure Stains; D010640:Phenothiazines; D010756:Phosphoric Acids; D013438:Sulfhydryl Compounds; D014048:Tolonium Chloride; C025818:Azure A; C005732:thioacetic acid