title: A technique for expressing eukaryotic genes in bacteria.
abstract: Methods are described that allow efficient expression in Escherichia coli of cloned eukaryotic genes. The methods require that the coding sequence of the gene in question be available in a form uninterrupted by intervening sequences (for example, as a complementary DNA clone). The gene products are synthesized unfused to other amino acid sequences. The genetic manipulations are simple, and require the plasmids described and commercially available enzymes.
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005914:Globins / Q000235:genetics; D007372:Interferons / Q000235:genetics; D009876:Operon; D010442:Peptide Chain Initiation, Translational; D010957:Plasmids; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D014764:Viral Proteins; D005914:Globins; D007372:Interferons