Skeletal muscle tissue preparation for image analysis systems.
A procedure for making histological sections of whole skeletal muscles suitable for automated image analysis of fiber size and number is described. Briefly the procedure involves the fixation/embedding technique of Schmitt and Dumont (1969) followed by section staining using phosphomolybdic acid and aniline blue. This procedure overcomes many of problems encountered with the more usual muscle section techniques in that it suppresses muscle fiber staining and stains only the intramuscular connective tissue.
D000814:Aniline Compounds; D000818:Animals; D001322:Autoanalysis; D002417:Cattle; D003238:Connective Tissue / Q000033:anatomy & histology; D005456:Fluorescent Dyes; D006652:Histological Techniques; D051379:Mice; D008982:Molybdenum; D009132:Muscles / Q000033:anatomy & histology*; D010756:Phosphoric Acids; D012756:Sheep; D013194:Staining and Labeling; D013690:Television
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D010756:Phosphoric Acids; C017006:aniline blue; D008982:Molybdenum; C003125:phosphomolybdic acid