Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB.
This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50--95% and the recovery was 30--40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests.
D000888:Antibodies, Anti-Idiotypic; D001491:Basophils / Q000378:metabolism; D001665:Binding Sites; D001783:Blood Flow Velocity; D002469:Cell Separation / Q000379:methods*; D002846:Chromatography, Affinity; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E; D011134:Polysaccharides; D012685:Sepharose / Q000031:analogs & derivatives
D000888:Antibodies, Anti-Idiotypic; D011134:Polysaccharides; C033056:anti-IgE-sepharose 6MB; D007073:Immunoglobulin E; D012685:Sepharose