Target antigens for antibodies and complement at the cell surface of RSV-transformed fibroblasts.
Using cells transformed by Rous sarcoma virus (RSV), an RNA tumour virus whose genetic and structural composition is fully known, the virus-induced surface antigens acting as targets for antibodies and complement were studied. Among the virus structural proteins, only the envelope antigen gp85, but not the core group-specific proteins or reverse transcriptase, were able to mediate immune lysis in the 51Cr-release assay. The group-specific antigenic determinants of gp85 were predominantly involved. The virus-induced cell surface antigen (VCSA), specific for transformation, was the only other molecule effective. Since different cells express either of these antigens, further support is given to the non-identity of virus structural antigens and VCSA.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001358:Avian Sarcoma Viruses / Q000276:immunology*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology; D014771:Virion / Q000276:immunology
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D000939:Epitopes; D003165:Complement System Proteins