Nucleic acid fixation to cyanuric chloride-activated paper. Use for nucleic acid transfer and affinity binding.
A procedure for the binding of nucleic acids to cyanuric chloride-activated paper is described. The pH value of the binding buffer has a substantial influence on the binding reaction and on the stability of binding. Substantial and stable binding of DNA occurs only in a pH range between 5 and 6. At pH 5.5 up to 20 microgram are bound per cm2 activated paper. Electrophoretically separated DNA and RNA are transferred to cyanuric chloride-activated paper using an electrotransfer method. The hybridization of transferred DNA and RNA to specific sequences is demonstrated.
D000818:Animals; D001665:Binding Sites; D010856:Columbidae; D004247:DNA / Q000302:isolation & purification*; D004563:Electrochemistry; D004590:Electrophoresis, Paper; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification*; D011817:Rabbits; D014227:Triazines
D014227:Triazines; C019309:cyanuric chloride; D012313:RNA; D004247:DNA