Role of polyamines in expression of the differentiated phenotype of chondrocytes in culture.
Addition of putrescine to cultures of rabbit costal chondrocytes during the log-phase stimulated expression of their differentiated phenotype, as judged by increase in the synthesis of glycosaminoglycans and of metachromasia with toluidine blue staining. Putrescine stimulated glycosaminoglycan synthesis maximally at a concentration of 10(-7) M, and spermidine and spermine were also effective at similar concentration. These polyamines had little effect on DNA synthesis, RNA synthesis, DNA accumulation, or protein accumulation. These findings suggest that polyamines may be important in the expression of the differentiated phenotype of chondrocytes in culture.
D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D006025:Glycosaminoglycans / Q000096:biosynthesis; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis; D010281:Parathyroid Hormone / Q000494:pharmacology; D010641:Phenotype; D011073:Polyamines / Q000502:physiology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D011817:Rabbits
D006025:Glycosaminoglycans; D010281:Parathyroid Hormone; D011073:Polyamines; D012313:RNA; D004247:DNA; D009955:Ornithine Decarboxylase