Motility and eosin uptake of formaldehyde-treated ram spermatozoa.
A concentration of 0.005% formaldehyde in phosphate-buffered saline (PBS) achieved complete immobilization of ram spermatozoa while also yielding good recovery of sperm motility after removal by washing. At a higher formaldehyde concentration (0.01%) recovery rate declined with increasing dilution rate. Incubation of spermatozoa in PBS containing 0.005% formaldehyde beyond 6 h at 5, 15 or 25 degrees C resulted in poor recovery rates. Of the incubation temperatures, eosin uptake was lowest at 25 degrees C. During 4 h post-wash incubation at 30 degrees C sperm motility was significantly (P less than 0.001) affected by pre-wash formaldehyde concentration which had no effect on the proportion of eosinophilic spermatozoa.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004801:Eosine Yellowish-(YS) / Q000378:metabolism*; D005557:Formaldehyde / Q000494:pharmacology*; D008297:Male; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D005557:Formaldehyde; D000469:Alkaline Phosphatase; D004801:Eosine Yellowish-(YS)