Comparative inhibition of nuclear RNA synthesis in cultured mouse leukemia L1210 cells by adriamycin and 4'-epi-adriamycin.
Adriamycin and 4'-epi-adriamycin were compared as to their effect on nRNA synthesis. 4'-Epi-adriamycin was a more effective inhibitor than the parent compound of RNA synthesis as measured by incorporation of [3H]-uridine. Adriamycin inhibited all three species of nRNA (ribosomal, non-poly(A)hnRNA, poly(A)hnRNA) to approximately the same extent. 4'-Epi-adriamycin on the other hand inhibited the nRNA species in the following order: non-poly(A)hnRNA greater than ribosomal RNA greater than poly(A)hnRNA. The inhibitory effects of both drugs on incorporation of uridine into RNA were reversible at low concentrations (5 microgram/ml).
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D015251:Epirubicin; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000096:biosynthesis; D013237:Stereoisomerism; D014529:Uridine / Q000378:metabolism
D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D015251:Epirubicin; D012313:RNA; D004317:Doxorubicin; D014529:Uridine