title: Ultrastructural studies of the nucleoli in diploid and trisomic chickens.
abstract: Chickens having a trisomic complement for the nucleolar organizer region (NOR) serve as a model for investigations on gene expression, molecular regulation, and physiological alterations resulting from extra genetic material. Of fundamental importance is the question of whether the extra set of ribosomal cistrons in trisomic chickens generates a morphologically and biochemically complete nucleolus. Ultrastructural and cytochemical studies of diploid and trisomic embryonic and adult cells were undertaken. All structures identified in trisomic cells as nucleoli by gross morphology were shown to be RNA rich by acridine orange fluorescence. At the ultrastructural level, the expected granular and fibrillar components were identified in all nucleoli of trisomic cells. Fibrillar centers, indicative of NOR's, were seen in all cases. These and other results from silver staining suggest full gene activity for each of the three chromosomes in the trisomic cell at all stages of development.
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure*; D002645:Chickens / Q000235:genetics*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region; D010641:Phenotype; D012313:RNA / Q000378:metabolism; D012834:Silver; D013194:Staining and Labeling; D014314:Trisomy
chemical_list: D012834:Silver; D012313:RNA