Effect of nerve activity on transport of nerve growth factor and dopamine beta-hydroxylase antibodies in sympathetic neurones.
The effect of nerve activity on the uptake and retrograde transport of nerve growth factor (NGF) and dopamine beta-hydroxylase (DBH) antibodies was studied by injecting 125I-labelled NGF and anti-DBH into the anterior eye chamber of guinea-pigs. Decentralization of the ipsilateral superior cervical ganglion (SCG) had no significant effect on the retrograde transport of either NGF or anti-DBH. Phenoxybenzamine produced a 50% increase in anti-DBH but not NGF accumulation and this effect was prevented by prior decentralization. This demonstrates that NGF is taken up independently of the retrieval of synaptic vesicle components.
D000320:Adrenergic Fibers / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001370:Axonal Transport; D003714:Denervation; D004299:Dopamine beta-Hydroxylase / Q000276:immunology*; D005728:Ganglia, Sympathetic / Q000378:metabolism; D006168:Guinea Pigs; D007498:Iris / Q000294:innervation; D009414:Nerve Growth Factors / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism; D009435:Synaptic Transmission
D000906:Antibodies; D009414:Nerve Growth Factors; D011942:Receptors, Adrenergic, alpha; D004299:Dopamine beta-Hydroxylase