title: Attachment of protein affinity-labeling reagents of variable length and amino acid specificity to E. coli tRNAfMet.
abstract: Transamination with bifunctional amines in the presence of bisulfite has been used to attach side chains of variable length to the N4-position of single stranded cytidine residues in E. coli tRNAfMet. Such side chains, terminating in reactive primary amino groups, have been coupled to a variety of N-hydroxysuccinimide esters. The resulting modified tRNAs carry protein affinity labeling groups capable of covalent reaction with a variety of amino acids.
mesh_terms: D000345:Affinity Labels; D000586:Amination; D000588:Amines; D000596:Amino Acids; D055598:Chemical Phenomena; D002621:Chemistry; D003562:Cytidine; D004926:Escherichia coli / Q000235:genetics*; D011506:Proteins / Q000032:analysis; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met
chemical_list: D000345:Affinity Labels; D000588:Amines; D000596:Amino Acids; D011506:Proteins; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D003562:Cytidine