Immunogenicity of ribosomes from enzymatically lysed Streptococcus pyogenes.
Ribosomal fractions isolated from Streptococcus pyogenes by physical and enzymatic disruption of the cell wall were found to provide protection in mice against challenge with the homologous M type. Although ribosomal fractions isolated by physical disruption of the cells also provided protection against challenge with several heterologous M types, ribosomal fractions from enzymatically lysed cells did not provide protection against any of the heterologous M types. Ribosomes isolated by either method were found to be contaminated with cell surface proteins. Chemical analysis of the ribosomes showed a greater protein:ribonucleic acid ratio in ribosomes from physically disrupted cells than in ribosomes from enzymatically disrupted cells (2:1 versus 1:1). Antisera to ribosomes isolated from physically disrupted cells detected many more antigenic determinants on ribosomes isolated from enzymatically disrupted cells than did the corresponding homologous antisera. Immunodiffusion analysis suggested that ribosomes isolated from physically disrupted cells may contain cell wall antigenic determinants which are present on ribosomes isolated from enzymatically disrupted cells in a partially degraded form. Washing of ribosomes in high-molarity salt solutions suggested that some of the contaminating cell wall proteins are tightly bound to the ribosomes.
D000818:Animals; D000942:Antigens, Bacterial; D001433:Bacteriolysis; D002458:Cell Fractionation / Q000379:methods; D000939:Epitopes; D007114:Immunization; D051379:Mice; D012270:Ribosomes / Q000032:analysis / Q000276:immunology*; D013290:Streptococcal Infections / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology* / Q000648:ultrastructure
D000942:Antigens, Bacterial; D000939:Epitopes