Hapten antibody production and the relevance of allogeneic reactions to elimination of the carrier effect.
Lewis (LEW) rats immunized 3 weeks before by injection of DNP-KLH together with Bordetella pertussis showed high levels of DNP antibody as judged by serum binding of 10(-7) M 3H-DNP-lysine 10 days after secondary immunization with DNP-KLH. Sera obtained from LEW rats following secondary immunization with DNP-BGG showed reduced DNP hapten binding. However, injection of 10(8) F1 hybrid Lewis X Brown Norway spleen cells into DNP-primed LEW rats 2 days before secondary immunization with DNP-BGG significantly increased the level of serum binding of 3H-DNP-lysine. These results provide evidence that the allogeneic cellular reaction associated with a host-versus-graft response induced by injection of F1 hybrid lymphoid cells into DNP-primed parental strain recipients partially obviates the requirement for carrier-specific T cells in the secondary anti-DNP response thereby providing a stimulus for triggering primed host B cells to produce antibody.
D000818:Animals; D000917:Antibody Formation; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle; D004136:Dinitrobenzenes / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007518:Isoantibodies; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D002352:Carrier Proteins; D004136:Dinitrobenzenes; D006241:Haptens; D007518:Isoantibodies; D005719:gamma-Globulins; D006433:Hemocyanins