Improved autoradiographic procedure for cytogenetics: inverted coverslips and double illumination.
The method described here has the advantage of presenting a clear image of both chromosomes and silver grains. Chromosomes are stained through the emulsion with Hoechst 33258. As two different sources of light are employed-epi-ultraviolet illumination and transmitted visible light-a separate photographic record of each optical plane can be obtained of chromosomes, silver grains, and chromosomes and grains together.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D006690:Bisbenzimidazole; D002875:Chromosomes / Q000378:metabolism / Q000648:ultrastructure*; D003582:Cytogenetics; D004261:DNA Replication; D008029:Lighting; D008297:Male; D008722:Methods; D009987:Orthoptera / Q000648:ultrastructure*; D012313:RNA / Q000096:biosynthesis; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D012313:RNA; D006690:Bisbenzimidazole