Cloning of malarial genes coding for high molecular weight antigens: isolation of fragments of Plasmodium falciparum genes coding for proteins of 145 000 molecular weight.
RNA preparations from Plasmodium falciparum were shown to direct the cell free synthesis of many high molecular weight proteins, some of which could be immunoprecipitated by sera from humans immune to P. falciparum. Among these was an abundant protein with a molecular weight of 145 000. Messenger RNAs coding for high molecular weight antigens were enriched by size fractionation and used after reverse transcription to identify genomic restriction fragments in Southern blots. Genomic restriction fragments were cloned into plasmid and phage lambda vectors. Two recombinants which were recognized by the high molecular weight probe were characterized by hybrid-select-translation. In both cases the predominant in vitro translation products were proteins with a molecular weight of 145 000.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular; D005796:Genes; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification
D000954:Antigens, Surface; D012313:RNA