Mouse monoclonal anti-N. I. Production and serological characterization.
Two examples of mouse monoclonal anti-N are described. The antibodies were derived from mice immunized with sialoglycoprotein extracts of group O MM ss erythrocyte membranes and the probable stimulus for immunization was glycophorin B associated N-antigen. Both antibodies reacted as direct agglutinins but appeared to recognize different epitopes with one having a greater dependence on sialic acid. The antibodies could prove to be valuable alternatives to those reagents used currently for N-blood grouping.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000939:Epitopes; D006021:Glycophorins / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008951:MNSs Blood-Group System / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006021:Glycophorins; D008951:MNSs Blood-Group System