A new procedure for the isolation of the brain myelin basic protein in a lipid-bound form.
Myelin basic protein has been isolated from bovine brain using the nonionic detergent n-octyl-polydisperse oligooxyethylene. The purified basic protein contains large amounts of heterogeneous lipids.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007202:Indicators and Reagents; D008055:Lipids / Q000302:isolation & purification*; D004676:Myelin Basic Protein / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification; D011485:Protein Binding
D006882:Hydroxyapatites; D007202:Indicators and Reagents; D008055:Lipids; D004676:Myelin Basic Protein; D010743:Phospholipids; D017886:Durapatite