Identification of a plasma membrane protein involved in Pi transport in the yeast Candida tropicalis.
A plasma membrane protein was found to contain antigenic determinants in common with a Pi-binding protein released by osmotic shock from Candida tropicalis. This plasma membrane protein (M.P. 30) has a molecular mass of 30 kDa as measured by SDS-PAGE and anti-M.P. 30 antibodies inhibit Pi uptake in protoplasts while only the corresponding Fab fragments inhibit Pi transport in whole cells. This plasma membrane protein may be the Pi plasma membrane carrier.
D000906:Antibodies / Q000276:immunology; D001692:Biological Transport; D002175:Candida / Q000032:analysis*; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis; D000939:Epitopes / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007163:Immunosorbent Techniques; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism
D000906:Antibodies; D002352:Carrier Proteins; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D028044:Phosphate-Binding Proteins; D010710:Phosphates