A monoclonal antibody to an oocyte-specific poly(A) RNA-binding protein.
Xenopus oocyte-specific poly(A) RNA-binding proteins were isolated and used to prepare monoclonal antibodies. One antibody was used to characterize one particular antigen by immunoblot analysis. The antigen had a molecular weight of 56,000 was oocyte-specific, and decreased in amount during oogenesis. The antigen was localized in the cytoplasm throughout oogenesis and sedimented mainly at 40-60 S. The antigen also was shown to bind poly(A) RNA following chromatography of ribonucleoprotein particles on oligo(dT)-cellulose. The antibody was used to immunoadsorb nontranslating ribonucleoprotein particles. Fifty-five per cent of the poly(A) RNA sedimenting between 40-60 S was shown to be bound by the antigen. The further use of this antibody in attempting to examine other components of the ribonucleoprotein particle is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D002846:Chromatography, Affinity; D004527:Egg Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D004527:Egg Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA