title: Inter-alpha-trypsin inhibitor (ITI): use of new antisera for qualitative studies and discrete quantitation of ITI and its derivatives.
abstract: Inter-alpha-trypsin inhibitor (ITI) is a human protease inhibitor characterized by its coexistence with several physiological derivatives displaying immunological cross-reactivity when analyzed in plasma with usual anti-ITI antisera. Taking advantage of the presence in urine of a particular ITI derivative, antisera with restricted specificity for either ITI or its derivatives could be prepared. Some applications of these new reagents are given, including qualitative studies and discrete quantitation--by electroimmunoassay--of ITI and ITI derivatives. The procedures herein described should prove useful for qualitative and quantitative analysis to investigators dealing with mixtures of antigenically related proteins.
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D006801:Humans; D007118:Immunoassay; D007120:Immunochemistry; D008970:Molecular Weight; D014357:Trypsin / Q000276:immunology / Q000378:metabolism
chemical_list: D000510:Alpha-Globulins; C011877:inter-alpha-inhibitor; D014357:Trypsin