Interaction between erythroblasts and macrophages in vitro: effect of neuraminidase-treatment of erythroblasts and the role of serum factors.
Some factors influencing the interaction in vitro between dimethylsulphoxide-induced Friend leukaemia erythroblasts (IFLE) and syngeneic mouse peritoneal macrophages (M phi) have been investigated. Desialation of erythroblasts by treatment with neuraminidase resulted in a significant increase in their association with and ingestion by M phi. In addition, the interaction with M phi of both neuraminidase-treated and untreated IFLE was found to be influenced by a heat-stable serum factor. gamma-globulin markedly enhanced the weak IFLE-M phi interaction which occurred at low serum concentration suggesting that the heat-stable factor may be an immunoglobulin. Desialation of the IFLE-rendered them agglutinable by peanut lectin which was used as a probe for neuraminidase-induced membrane changes. By contrast, cycloheximide treatment of IFLE which also enhances their interaction with M phi, did not result in the exposure of receptors for this lectin. It is proposed that both desialation and cycloheximide treatment of IFLE lead to secondary alterations in their membrane structure which renders them recognizable by M phi.
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D004900:Erythroblasts / Q000187:drug effects / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000097:blood; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D005719:gamma-Globulins / Q000494:pharmacology
D001798:Blood Proteins; D037102:Lectins; D005719:gamma-Globulins; D009439:Neuraminidase