Human Ia beta chains and the invariant chain share a common antigenic determinant.
We have presented evidence that a mouse monoclonal antibody 1,227, which was previously shown to recognize a determinant on the light chains beta 1 and beta 2 of human Ia antigens, also recognizes a determinant on the invariant chain (1) associated with these molecules. Ia-specific proteins were resolved by two-dimensional (2D) PAGE and electrophoretically transferred onto nitrocellulose filters. The presence of a determinant shared between beta 1, beta 2 and I was established using "Western blotting" technique. In addition, we demonstrated that 1,227 can immunoprecipitate isolated beta 1, beta 2, and the invariant chain proteins following their separate elution from SDS-PAGE.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II