In vitro immunization for the production of antigen-specific lymphocyte hybridomas.
Antigen-specific lymphocyte hybridomas have been produced by a simple in vitro immunization procedure. Non-immune spleen cells have been activated in vitro, and the B-cell blasts have subsequently been used for somatic cell hybridization. Hybrids secreting monoclonal antibodies to human myoglobin, pig insulin, and benzo(a)pyrene were obtained with splenocytes that had been immunized 5 days in culture before the hybridization. The frequency of specific hybridomas was the same as or higher than that of hybridomas produced from in vivo-immunized cells, showing the versatility of the procedure when making monoclonal antibodies to haptens and proteins with a conserved structure or to small amounts of antigen. Monoclonal antibodies of both IgG and IgM isotypes could be isolated.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D002478:Cells, Cultured; D000939:Epitopes; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D007330:Insulin Antibodies / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009211:Myoglobin / Q000276:immunology; D013552:Swine
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007330:Insulin Antibodies; D009211:Myoglobin