Trinitrophenyl modification of H-2k and H-2b spleen cells results in enhanced serological detection of Kk-like determinants.
Several anti-H-2Kk but not anti-H-2Dd monoclonal antibodies (mAb) exhibited enhanced binding to B10.A murine spleen cells after modification of the cells with trinitrobenzene sulfonate (TNBS). The number of antibody molecules bound to TNP-modified B10.A spleen cells increased by a factor of two or more. The same anti-2Kk mAb that exhibited enhanced binding to modified B10.A cells did not bind to unmodified C57BL/10 spleen cells, as expected, but did bind to TNP-modified C57BL/10 spleen cells. This TNP-dependent binding was not a result of cross-reactions with cell surface TNP groups nor with Fc receptors. TNP modification of a variant cell line that does not express class I H-2 products did not result in enhanced binding by these mAb. These findings can account for preferential recognition of TNP-Kk by B10.A and B10.BR CTL, and also for cross-reactive lysis by C57BL/10 CTL stimulated by C57BL/10-TNP against unmodified H-2Kk targets.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D064532:Histocompatibility Antigen H-2D; D007519:Isoantigens / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology* / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D006241:Haptens; D064532:Histocompatibility Antigen H-2D; D007519:Isoantigens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes