Control of H-2 antigen and beta 2-microglobulin gene expression in mouse trophoblast cell clones.
We have investigated the expression of H-2 antigen and beta 2-microglobulin in recently established mouse trophoblast cell clones. These clones, derived from C57BL/6 (H-2b) or BALB/c (H-2d) strains, synthesized extra-embryonic endoderm- and trophectoderm-specific cytoskeletal proteins, termed "Endo A" and "Endo B," and no detectable SSEA-1 embryonic antigen. Flow microfluorometry indicated that H-2 antigen expression on the cell surface of trophoblast cells was very low, corresponding to 2-5% of the amounts found on differentiated teratocarcinoma cells (12-1a) and BALB/c 3T3 cells, respectively. Expression of beta 2-microglobulin was reduced to approximately equal to 14% of the amounts found on 12-la cells. Immunoprecipitation and polyacrylamide gel electrophoretic analysis indicated that the synthesis of H-2 antigen and beta 2-microglobulin in the trophoblast cells was lower than that found in normal spleen cells. In addition low, but unequal, levels of mRNA specific for H-2 antigen and beta 2-microglobulin were found in trophoblast cells by blot hybridization with cDNA probes. The low mRNA levels may be due to transcriptional control of the genes encoding H-2 antigen and beta 2-microglobulin.
D000818:Animals; D001609:Beta-Globulins / Q000235:genetics*; D002999:Clone Cells / Q000032:analysis; D005260:Female; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D013499:Surface Properties; D013724:Teratoma / Q000032:analysis; D014327:Trophoblasts / Q000032:analysis*; D001613:beta 2-Microglobulin / Q000235:genetics*
D001609:Beta-Globulins; D006183:H-2 Antigens; D012333:RNA, Messenger; D001613:beta 2-Microglobulin