Enzyme immunoassay for pregnancy-associated plasma protein-A.
This enzyme immunoassay procedure for pregnancy-associated plasma protein type A involves a "sandwich"-type system in microtitration plates. One can detect 0.27 mg of the protein per liter of serum, with a between-batch CV of 10.2%, and the antiserum used does not cross react with any of the other placentally derived proteins. A reference interval for the last trimester of pregnancy is presented. The procedure described is suitable for studying the behavior of this protein during pregnancy.
D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis* / Q000097:blood; D011263:Pregnancy Trimester, Third; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis*; D011347:Procollagen / Q000097:blood*; D012016:Reference Values
D011257:Pregnancy Proteins; D011347:Procollagen; D011266:Pregnancy-Associated Plasma Protein-A