Basilea kappa light chains of rabbit immunoglobulins.
Immunogenetics of the Basilea allotype were investigated. It was shown that the Basilea gene is controlled at a locus identical with, or closely linked to, the Ab kappa allotypic locus. Basilea-positive molecules have been physically separated by immunoabsorption from molecules carrying the lambda chain markers, c7 and c21. Injection of newborn Basilea homozygotes with anti-Basilea immune serum results in suppression of the synthesis of Basilea-positive molecules, but does not affect the synthesis of the lambda chain, or of the heavy chain allotypic markers. Allotype-suppressed Basilea homozygotes do not recognize either the Basilea allotype or the kappa isolate as 'self' and make anti-Basilea and anti-kappa antibody upon immunization with Basilea antigen. The specificity of this antibody was identical with that of a heterologous anti-kappa immune serum raised in a goat and made specific by absorption with purified lambda material. These results prove that Basilea gene products are of kappa isotype and are controlled at the Ab allotypic locus.
D000818:Animals; D000831:Animals, Newborn; D002846:Chromatography, Affinity; D000939:Epitopes / Q000032:analysis / Q000235:genetics / Q000276:immunology; D005260:Female; D006041:Goats; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology; D007126:Immunoglobulin Allotypes / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D008297:Male; D011817:Rabbits / Q000235:genetics*
D000939:Epitopes; D007106:Immune Sera; D007126:Immunoglobulin Allotypes; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains