The 13p HSR in a human neuroblastoma cell line does not contain rRNA genes.
We have studied a human neuroblastoma cell line containing a homogeneously staining region (HSR) on the short (p) arm of chromosome #13. The HSRs are believed to represent sites of gene amplification, containing multiple copies of one or a small number of genes. The 18S and 28S ribosomal RNA genes are the only structural genes so far assigned to the p arms of the human acrocentric chromosomes, which include number #13. Using two recombinant DNA probes specific for sequences in the 18S/28S ribosomal RNA (rRNA) gene complex for in situ hybridization, we have been able to establish that the 13pHSR in this line does not contain rRNA genes.
D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002901:Chromosomes, Human, 13-15; D004247:DNA / Q000235:genetics*; D004275:DNA, Ribosomal; D005796:Genes; D006801:Humans; D007621:Karyotyping; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D012335:RNA, Ribosomal / Q000235:genetics*; D013194:Staining and Labeling
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004247:DNA