Effects of intratracheal administration of bleomycin on GSH-shuttle enzymes, catalase, lipid peroxidation, and collagen content in the lungs of hamsters.
Intratracheal administration of one unit of bleomycin increased significantly the glucose-6-phosphate dehydrogenase activity to 147, 135, 163, 207, 278, and 148% of the control at 1, 2, 4, 7, 14, and 21 days post-treatment, respectively. The glutathione peroxidase activity was significantly increased to 147 and 124% of the control at 7 and 14 days post-treatment, respectively. The glutathione reductase and catalase activities were significantly elevated to 132, 174, and 145% of the control in the former and 160, 213, and 160% of the control in the latter case at 7, 14, and 21 days post-treatment, respectively. The amount of thiobarbituric acid reacting products as an index of lipid peroxidation was found to be significantly increased to 251, 248, and 253% of the control at 4, 7, and 14 days after treatment, respectively. The amount of total lung collagen in treated hamsters was significantly increased to 140, 197, and 245% of the control at 7, 14, and 21 days post-treatment, respectively. These findings indicate that the increases in the activities of antioxidant enzymes in lungs of bleomycin-treated hamsters result from the multiple cyto-dynamic events such as infiltration of inflammatory cells, and reparative and proliferative processes which generally follow lung injury caused by any oxidant.
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002374:Catalase / Q000032:analysis; D003094:Collagen / Q000032:analysis; D006224:Cricetinae; D005952:Glucose-6-Phosphatase / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D008054:Lipid Peroxides / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D013482:Superoxide Dismutase / Q000032:analysis
D008054:Lipid Peroxides; D001761:Bleomycin; D003094:Collagen; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005952:Glucose-6-Phosphatase