A simple, rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and methylene blue.
Sections of tissue embedded in glycol methacrylate can be stained in rapid sequence with solutions of 1% aqueous chromotrope 2R adjusted to pH 3 and 0.1% methylene blue to produce sufficient contrast and cellular detail to permit quick visual inspection and/or photomicrography. Solutions of these stains are simple to prepare and are stable over long periods. Staining of sections may be accomplished within six minutes.
D000818:Animals; D004847:Epithelial Cells; D000882:Haplorhini; D007672:Kidney Cortex / Q000166:cytology; D008689:Methacrylates; D008751:Methylene Blue; D009132:Muscles / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*
D008689:Methacrylates; D008751:Methylene Blue