Characterization of ribonucleic acid transcriptase in Ibaraki virus core particles.
Ibaraki virus core particles were purified from infected BHK-21 cells. The core particles displayed RNA transcriptase activity while the virion did not. The RNA transcriptase activity was stimulated about 15-fold by addition of S-adenosyl-L-methionine. The reaction product was single-stranded RNA which could be hybridized with heat-denatured Ibaraki virus RNA. The hybrids possessed the same electrophoretic mobility as Ibaraki virus double-stranded RNA.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D012330:RNA, Double-Stranded / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism*; D012087:Reoviridae / Q000201:enzymology*; D014158:Transcription, Genetic
D012330:RNA, Double-Stranded; D012194:RNA-Directed DNA Polymerase