T cells produce an antigen-binding factor with in vivo activity analogous to IgE antibody.
T cell-dependent activation of resident tissue mast cells is required for the elicitation of delayed-type hypersensitivity skin reactions in mice. A T cell-derived antigen-binding factor that transfers the ability to elicit an immediate hypersensitivity-like skin reaction is described and compared with a hybridoma IgE antibody. Both the T cell factor and IgE mediate reactions with increased vascular permeability and both are mast cell dependent, as they are inactive in two different types of mast cell deficient mice (W/Wv and Sl/Sld). The T cell factor was distinguished from IgE by affinity chromatography using specific anti-IgE and anti-factor antibodies and by a shorter duration of passive sensitization. The T cell factor is a suitable candidate for participation in the mechanism by which T cells activate mast cells in delayed-type hypersensitivity.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D000939:Epitopes; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000276:immunology; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains; D010081:Oxazolone / Q000276:immunology; D010853:Picryl Chloride / Q000276:immunology; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; C026408:anti-IgE antibodies; D010081:Oxazolone; D007073:Immunoglobulin E; D010853:Picryl Chloride