Monoclonal antibodies against spermatozoa of the common carp (Cyprinus carpio L.). I. A study germ cell antigens in adult males and females.
Eleven monoclonal antibodies that recognize membrane determinants on spermatozoa of the carp Cyprinus carpio L. have been produced. Indirect immunofluorescence revealed that these determinants are uniformly distributed on the surface of head and midpiece. Most of them are also present on the outer membrane of precursor sperm cells. Although none of the monoclonal antibodies reacted with carp somatic tissue, five monoclonal antibodies were positive for surface membrane determinants of oogonia and early prophase oocytes in carp ovary. Preliminary analysis of the testis and ovary of three other species of fish showed that some carp determinants are shared with germ cells from Barbus conchonius, Clarias lazera, or Salmo gairdneri.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002347:Carps; D002462:Cell Membrane / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D008855:Microscopy, Electron, Scanning; D010053:Ovary / Q000166:cytology* / Q000648:ultrastructure; D012741:Sexual Maturation; D013094:Spermatozoa / Q000032:analysis* / Q000276:immunology; D013737:Testis / Q000166:cytology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes