Fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein D.
The fine structure of the antigenic determinants of herpes simplex virus type 1 and 2 glycoprotein D (gD) was analyzed to determine whether structural differences underlie the differential immunogenicity of these glycoproteins. A region common to herpes simplex virus type 1 and 2 gD (amino acid residues 11 to 19) and two sites specific for herpes simplex virus type 2 gD (one determined by proline at position 7, the other determined by asparagine at position 21) were localized within the N-terminal 23 amino acids of gD by synthesis of peptides and comparison of their cross-reactivity with antisera raised to herpes simplex virus type 1 and 2 gD. The secondary structure of these peptides, as predicted by computer analysis, is discussed in relation to their immunogenicity.
D000595:Amino Acid Sequence; D000939:Epitopes / Q000032:analysis*; D007106:Immune Sera; D011485:Protein Binding; D011487:Protein Conformation; D018139:Simplexvirus / Q000032:analysis*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C039447:glycoprotein D, Human herpesvirus 1; C040570:glycoprotein D-herpes simplex virus type 2