[Rheologic activation of blood platelets. Preliminary study].
A Couette type coaxial cylinder viscosimeter was used to evaluate the influence of deformation rates up to 20,000/s on blood platelets, in the presence or absence of red cells. Plasma lactate dehydrogenase and haemoglobin levels were determined in order to assess cell lysis and the estimation of beta-thromboglobulin (beta-TG) was considered to be an index of platelet activation. Results for whole blood revealed a change in red cells from 10,000/s upwards, which increased as the rate rose. At the same time, beta-TG rose slowly from the lowest deformation rates then considerably, in relation with the liberation of erythrocytic substances. The rise in LDH and beta-TG levels in platelet rich plasma (PRP) remained small up to 7,000/s approximately, then increased very rapidly, suggesting earlier mechanical and biochemical activation than in whole blood.
D001609:Beta-Globulins / Q000032:analysis*; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D010974:Platelet Aggregation; D011863:Radioimmunoassay; D012212:Rheology; D001620:beta-Thromboglobulin / Q000032:analysis*
D001609:Beta-Globulins; D006454:Hemoglobins; D001620:beta-Thromboglobulin; D007770:L-Lactate Dehydrogenase
Activation rhéologique des plaquettes sanguines. Etude préliminaire.