Effects of bleomycin on kinetoplast DNA and nuclear DNA in Trypanosoma gambiense using in situ microfluorometry technique.
The effect of Bleomycin, an anti-tumour antibiotic, on either kinetoplast DNA(K-DNA) or nuclear DNA(N-DNA) in a single Trypanosoma gambiense cell was measured successfully utilizing the in situ microfluorometry technique. This microfluorometric assay clearly revealed that the relative fluorescence intensity (RFI) of K-DNA and N-DNA was greatly reduced by bleomycin, suggesting the occurrence of the single strand breaks in these DNAs. The present results have shown that bleomycin acts on N-DNA stronger than K-DNA at least in Trypanosoma gambiense. Microfluorometry technique can be employed valuably for studying the DNA damages of several anti-protozoan drugs.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology* / Q000627:therapeutic use; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009940:Organoids / Q000187:drug effects / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D014347:Trypanosoma brucei gambiense / Q000187:drug effects* / Q000378:metabolism; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000469:parasitology*
D004270:DNA, Circular; D001761:Bleomycin; D004247:DNA