Characterization of two substance P antisera.
The cross reactivity of two currently used substance P (SP) antisera (K 16 and K 25) with various analogues of SP, partial sequences of SP and other naturally occurring peptides were tested in a radioimmunoassay system. Both antisera were found to be C-terminal directed, this tendency being most pronounced for antiserum K 16. The capacity of antiserum K 25 to bind various SP-related peptides correlated to the previously described biological activity of these peptides on the isolated guinea pig ileum. The results thus indicate that this antiserum binds to a biologically active part of the SP-molecule.
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002096:C-Peptide / Q000378:metabolism; D003429:Cross Reactions; D007106:Immune Sera; D010443:Peptide Chain Termination, Translational; D011863:Radioimmunoassay; D013373:Substance P / Q000276:immunology*
D002096:C-Peptide; D007106:Immune Sera; D013373:Substance P