The localisation of the 5'-termini of in vivo transcripts of a cloned rainbow trout protamine gene.
The mRNA start site of a cloned rainbow trout protamine gene (TPG-3) has been localised using S1-nuclease mapping and primer extension of in vivo synthesised trout testis poly A+-RNA. The presumptive cap site occurs within an AT-rich region, only 14 nucleotides from the start of the protein-coding sequence. Transcription of this protamine gene in vitro, using the Hela whole-cell extract system, generates products initiated at the same nucleotide as that used in vivo. In vitro transcription is abolished by deletion of sequences between -20 and -48, within which is a canonical TATA-box having an llbp homology with the strong chick conalbumin and Adenovirus-2 major late promoters (CTATAAAAGGG).
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis; D005796:Genes; D011061:Poly A / Q000235:genetics; D011479:Protamines / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012487:Salmonidae / Q000235:genetics*; D014158:Transcription, Genetic; D014337:Trout / Q000235:genetics*
D004274:DNA, Recombinant; D011479:Protamines; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes