Fluctuations in phosphoribosyl pyrophosphate levels in monolayer tumor cell lines. Effects of drugs.
The concentration of PRPP (phosphoribosyl pyrophosphate) measured in tumor cells grown in monolayer showed a large variation with the various harvesting methods examined, including trypsinization. This variation could be reduced by a 1-h incubation of trypsinized cells as a suspension in Dulbecco's medium. After this preincubation these cell suspensions were suitable for the study of modulation of PRPP. One microM methotrexate caused a 2-3-fold increase and 1 mM N- phosphonoacetyl -L-aspartate a slight increase, but inosine and deoxyinosine drastically reduced PRPP concentrations. 5-Fluorouracil had no effect. This study demonstrates that metabolic parameters such as PRPP concentrations can be studied conveniently in suspensions of cells which are commonly cultivated as monolayers.
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002021:Buffers; D002460:Cell Line; D003584:Cytological Techniques; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D010428:Pentosephosphates / Q000378:metabolism*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism*; D011684:Purine Nucleosides / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D002021:Buffers; D010428:Pentosephosphates; D011684:Purine Nucleosides; D001224:Aspartic Acid; D010754:Phosphoribosyl Pyrophosphate; C013195:sparfosic acid; D014357:Trypsin; D010746:Phosphonoacetic Acid; D008727:Methotrexate