Sheep gastric mucins as a source of blood-group-I and -i antigens.
Gastric-mucosal glycoproteins of sheep have been shown to express the blood-group-I and -i antigens. The highest blood-group-I activities were found in glycoproteins lacking in blood-group-A and -H activities. In antigenically very active glycoprotein preparations, approx. 25% of the macromolecules expressed various blood-group-I and -i antigens, and these could be enriched by affinity chromatography with an anti-(blood group I) immunoadsorbent column. Additional blood-group-I and -i activities could be revealed by one cycle of Smith degradation of blood-group-A-active sheep gastric glycoproteins. Thus sheep gastric mucins are an abundant source of oligosaccharides with blood-group-I- and -i-active sequences.
D000818:Animals; D001789:Blood Group Antigens; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods; D000939:Epitopes / Q000032:analysis; D005753:Gastric Mucosa / Q000276:immunology*; D006023:Glycoproteins / Q000302:isolation & purification; D007048:I Blood-Group System; D009077:Mucins / Q000276:immunology*; D009844:Oligosaccharides / Q000032:analysis; D012756:Sheep
D001789:Blood Group Antigens; D000939:Epitopes; D006023:Glycoproteins; D007048:I Blood-Group System; D009077:Mucins; D009844:Oligosaccharides