Cellulolytic activity of moulds. II. Various methods of precipitating and concentrating enzymes and their influence on the activity of cellulolytic preparation and xylanase of Aspergillus terreus F-413.
Complexes of cellulolytic enzymes and xylanase were precipitated and concentrated by various methods from post-culture liquids of Aspergillus terreus F-143, containing cellucotton as carbon source. The best results in regard to the specific activity of the preparations were obtained by precipitation of enzymes with acetone-denatured ethanol. Besides high cellulolytic and xylanase activity the crude enzyme preparation showed the presence of small amounts of amylase, protease and polygalacturonase.
D000096:Acetone / Q000494:pharmacology; D000645:Ammonium Sulfate / Q000494:pharmacology; D001230:Aspergillus / Q000201:enzymology*; D002480:Cellulase / Q000302:isolation & purification*; D002482:Cellulose / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods; D011232:Chemical Precipitation; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology; D005959:Glucosidases / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods; D043325:Xylan Endo-1,3-beta-Xylosidase; D001617:beta-Glucosidase / Q000302:isolation & purification*
D003470:Culture Media; D000096:Acetone; D000431:Ethanol; D002482:Cellulose; D005959:Glucosidases; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase; D000645:Ammonium Sulfate