The relationship between adjuvant and mitogenic effects of amphotericin methyl ester.
The antifungal polyene amphotericin B (AmB) and its methyl ester derivative (AME) both show potent murine immunostimulant as well as B-cell activating effects. Under certain experimental conditions, AME is a much more potent polyclonal B-cell activator (PBA) than AmB. Notable features of the murine B-cell stimulation induced by AME include: (i) High concentrations of AME (50-100 microgram/ml) are required and even at this level exhibit little or no spleen cell toxicity. (ii) Several lines of evidence suggest that the B-cell activating properties of AME are not involved in the cellular mechanism of adjuvant activity in vivo. (iii) There is a strong correlation between the magnitude of the in vitro PBA effects and the in vivo adjuvant effects of AME in a survey of different mouse strains. This evidence suggests that there is genetic control of the murine lymphoid cell-stimulatory effects of AME and that a small number of genes determines the responsive phenotype.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000666:Amphotericin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000032:analysis; D002452:Cell Count; D005260:Female; D005802:Genes, MHC Class II; D006462:Hemolytic Plaque Technique; D007075:Immunoglobulin M / Q000032:analysis; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology; D000511:alpha-Macroglobulins / Q000235:genetics / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007075:Immunoglobulin M; D000511:alpha-Macroglobulins; C032306:polyclonal B cell activator; C017501:methylamphotericin B; D000666:Amphotericin B