Heparin formation and mechanism of its action as growth promoter.
Heparin was prepared and purified by two methods. Rf value, elemental and chemical analyses of our isolate coincided with the clinically used heparin. Heparin stimulated mitotic activities in Escherichia coli B and Aspergillus ochraceus. This was evident from the increase in DNA, RNA, and protein synthesis. The mechanism of growth stimulation by heparin probably takes place as follows: Heparin associates with nuclear protein, releasing DNA from DNP and thus stimulates DNA synthesis. Heparin was taken up by the cells of A. flavus, A. niger, and A. ochraceus. The uptake of heparin increased with time and was affected by several factors, such as pH, different salts, and heparin concentration.
D001230:Aspergillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D004271:DNA, Fungal / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D006493:Heparin / Q000302:isolation & purification / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012331:RNA, Fungal / Q000096:biosynthesis; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004271:DNA, Fungal; D005656:Fungal Proteins; D012329:RNA, Bacterial; D012331:RNA, Fungal; D006493:Heparin