Cytokeratin filament expression during in vitro teratocarcinoma cell differentiation as detected by a monoclonal antibody.
Undifferentiated F9 teratocarcinoma cells were induced to differentiate in culture using retinoic acid and cAMP. As a result, the morphology of the cultures changes dramatically. Using a monoclonal antibody directed against cytokeratin polypeptide 18 (RGE 53) in the indirect immunofluorescence technique we could show that this cytokeratin subunit is synthesized and assembled into a filamentous network upon differentiation in about 50% of the cells. Immunoblotting studies confirm these results.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis* / Q000276:immunology; D007633:Keratins / Q000096:biosynthesis* / Q000276:immunology; D008113:Liver Neoplasms; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000276:immunology; D013724:Teratoma / Q000378:metabolism* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D007633:Keratins