Affinity partitioning of albumin and alpha-fetoprotein in an aqueous two-phase system using poly(ethylene glycol)-bound triazine dyes.
Human albumin and alpha-fetoprotein are partitioned in an aqueous two-phase system composed of 10% (w/w) Dextran and 7.5% (w/w) poly(ethylene glycol). When a small amount of poly(ethylene glycol) is replaced by Cibacron Blue F3G-A-liganded poly(ethylene glycol) the partition coefficient, K, of albumin increases by the factor of about 4000 whereas the K value of alpha-fetoprotein undergoes only a small change. The change of the partition coefficient in a logarithmic scale induced by increasing dye-polymer concentrations turned out as a useful measure for the affinity of albumin and alpha-fetoprotein to the dyes. The effect of pH and salt concentration on the affinity partition of albumin and alpha-fetoprotein is demonstrated. The partition of the two proteins in presence of Cibacron Blue F3G-A-liganded poly(ethylene glycol) is compared with seven other triazine dye-poly(ethylene glycol) derivatives.
D002606:Charcoal; D002846:Chromatography, Affinity / Q000379:methods; D004396:Coloring Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D011092:Polyethylene Glycols; D012709:Serum Albumin / Q000032:analysis*; D014227:Triazines; D000509:alpha-Fetoproteins / Q000032:analysis*
D004396:Coloring Agents; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D014227:Triazines; D000509:alpha-Fetoproteins; D002606:Charcoal; D011092:Polyethylene Glycols