Immunological study of a cellular 35K phosphorylated polypeptide detected in Rous sarcoma virus transformed cells.
We have immunoprecipitated a phosphoprotein of 35K daltons (35K) common to RSV-transformed chick embryo fibroblasts (CEF) and rodent cells. The phosphorylation of this antigen depends on the expression of the v-src gene and contains phosphotyrosine. The pre-existing 35K protein, of CEF infected de novo, was further shown to become phosphorylated shortly after the appearance of active pp60v-src, and about 1 day before morphological transformation. The experiments with RSV-transformed rodent cells have shown that the 35K phosphoprotein is associated with the cellular framework. Another phosphoprotein of 37K was found in the RSV-transformed rodent cells, but not in the transformed CEF. However, this protein was not phosphorylated at tyrosine residues and its phosphorylation persisted, at the restrictive temperature, in cells transformed by a ts mutant of RSV.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D003370:Coturnix; D006224:Cricetinae; D003599:Cytoskeleton / Q000032:analysis; D015688:Oncogene Protein pp60(v-src); D010750:Phosphoproteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis; D014764:Viral Proteins / Q000378:metabolism*
D010750:Phosphoproteins; D014764:Viral Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)