Ability of lithium to accelerate the recovery of granulopoiesis after subacute radiation injury.
Lithium stimulates granulopoietic recovery after mice are exposed to 2 Gy. By examining the hematopoietic inductive microenvironment (HIM) using the stromal colony assay, we demonstrate here that lithium, during the two weeks after irradiation, produced less stromal colony suppression than was observed from the irradiated controls. Recovery peaked by day 19 and returned to normal by day 28. This response was also observed in splenic derived stroma. Furthermore, stroma from lithium-irradiated animals supported the in vitro growth of granulocyte-macrophage colonies (CFU-GM) greater than observed from irradiated controls. These data suggest lithium accelerates granulopoietic recovery by first providing for a completely reconstituted and functional HIM.
D000818:Animals; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D006410:Hematopoiesis / Q000187:drug effects / Q000528:radiation effects*; D008094:Lithium / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D008297:Male; D051379:Mice; D009283:Naphthol AS D Esterase / Q000032:analysis; D011833:Radiation Injuries, Experimental / Q000097:blood*; D013194:Staining and Labeling; D014916:Whole-Body Irradiation
D008094:Lithium; D009283:Naphthol AS D Esterase