Enzyme immunometric assay for the determination of pregnancy associated plasma protein A (PAPP-A) with the antigen as solid phase (conjoint IEMA).
Purified pregnancy associated plasma protein A (PAPP-A) can be effectively bound to polystyrene microtitre plates. This immobilized antigen competes with the added serum PAPP-A of unknown concentration for the limited amount of peroxidase-labeled monospecific anti-PAPP-A antibody incubated simultaneously. The sensitivity is 0.1 WHO unit/ml and non-specific binding is 1.0%.
D000941:Antigens; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011137:Polystyrenes; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis*
D000941:Antigens; D011137:Polystyrenes; D011257:Pregnancy Proteins; D011266:Pregnancy-Associated Plasma Protein-A