title: Sequence of centromere separation: orderly separation of multicentric chromosomes in mouse L cells.
abstract: Mouse L cells have many dicentric chromosomes and one with eight centromeres. All eight centromeres behave similarly until midmetaphase when most centromeres split into two units each in apparently quick succession but out-of-phase. This premature separation leaves one or perhaps two closely located centromeres intact, which separate at late metaphase-anaphase, drawing the two chromatids to opposite poles. Such dominance of one centromere over all others, though unexplained, ensures the lack of any mitotic abnormality such as bridges or fragments. These observations show that all the centromeres are retained as functional primary constrictions except for a change in functional regulation when more than one centromere are located on a chromosome.
mesh_terms: D000818:Animals; D006690:Bisbenzimidazole / Q000494:pharmacology; D002503:Centromere / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D003703:Demecolcine / Q000494:pharmacology; D007621:Karyotyping; D007739:L Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects; D051379:Mice
chemical_list: D006690:Bisbenzimidazole; D003703:Demecolcine