title: Indirect evidence of cell wall alterations in Pseudomonas aeruginosa by immunoglobulin preparations.
abstract: Ten strains of Pseudomonas aeruginosa isolated from different clinical sources were incubated with immunoglobulin preparations for i.v. use. This resulted in the release of beta-lactamases normally cell-bound in the periplasmic space. The amount of released beta-lactamases - depending on the degree of cell wall alteration - was spectrophotometrically determined by the use of nitrocefin as an indicator substance. beta-lactamase release varies according to strain specificity and depends on the chemical modification of the immunoglobulins used.
mesh_terms: D002473:Cell Wall / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000031:analogs & derivatives* / Q000494:pharmacology*; D016756:Immunoglobulins, Intravenous; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000648:ultrastructure*; D001618:beta-Lactamases / Q000378:metabolism; D005719:gamma-Globulins / Q000494:pharmacology*
chemical_list: D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D005719:gamma-Globulins; C031151:pepsinated immunoglobulin G; D001618:beta-Lactamases