Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
The presence of cellular aggregates in cell suspensions derived from human solid tumours often complicates subsequent evaluation of colony formation in primary soft agar cultures (Agrez et al., 1982b). In the present study, performance of a conventional colony formation assay was observed to lack sufficient sensitivity to identify growth and active chemotherapeutic agents in the majority of specimen cultures. Modification of conventional methodologies to include filtration of cell suspensions, use of "proliferation control" and "cytotoxicity control" cultures as well as vital staining were found to be essential for the valid assessment of primary soft agar cultures in our laboratory. In addition, application of drugs to culture surface in place of culture incorporation appeared to facilitate culture performance and drug sensitivity testing.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D003114:Colony-Forming Units Assay; D005260:Female; D005374:Filtration / Q000379:methods; D006801:Humans; D008297:Male; D008722:Methods; D009369:Neoplasms / Q000473:pathology*; D012685:Sepharose; D019810:Sodium Azide; D013194:Staining and Labeling / Q000379:methods; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents; D001386:Azides; D012685:Sepharose; D019810:Sodium Azide