title: Determination of nine catecholamine metabolites and 5-hydroxyindolacetic acid in urine by capillary gas chromatography.
abstract: A method is described for the simultaneous determination of nine urinary acidic and alcoholic catecholamine metabolites and urinary 5-hydroxyindolacetic acid. Incubation of a urine sample in the presence of ascorbic acid, glucuronidase and acylase and subsequent extraction with ethyl acetate precedes derivatization to trimethylsilyl compounds, capillary gas chromatographic separation and flame-ionization detection. The automated dual injection procedure and the analytical characteristics of the proposed method are reported in detail. Special attention is paid to problems that occur in analysis on a routine basis.
mesh_terms: D000293:Adolescent; D000328:Adult; D001205:Ascorbic Acid; D001322:Autoanalysis; D002395:Catecholamines / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods; D006626:Hippurates / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D008875:Middle Aged; D013048:Specimen Handling
chemical_list: D002395:Catecholamines; D006626:Hippurates; D006897:Hydroxyindoleacetic Acid; D001205:Ascorbic Acid; C030514:hippuric acid