Inhibition of active transport and macromolecular synthesis by pyocin 103 in Neisseria gonorrhoeae.
The mechanism of inhibition of Neisseria gonorrhoeae by pyocin 103 was examined. Pyocin 103 decreases the viability of N. gonorrhoeae immediately after its addition to logarithmically growing cells by single-hit kinetics. This inhibition was paralleled by an immediate cessation of protein synthesis, RNA synthesis, and active transport. From the data presented, it is likely that pyocin kills N. gonorrhoeae in the same manner that it kills Pseudomonas aeruginosa, i.e., by interfering with energy metabolism.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001430:Bacteriocins / Q000494:pharmacology*; D001692:Biological Transport; D003470:Culture Media; D008715:Methionine / Q000378:metabolism; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011709:Pyocins / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis*
D001426:Bacterial Proteins; D001430:Bacteriocins; D003470:Culture Media; D011709:Pyocins; D012329:RNA, Bacterial; D008715:Methionine