Cloning of metallothionein cDNA from neonatal rat liver.
A metallothionein cDNA clone was isolated from a cDNA bank prepared from neonatal rat liver poly(A)-containing RNA by a colony screening procedure using [32P]cDNA probes prepared from mRNA of either metal-induced or uninduced rat livers. Nucleotide sequence analysis of this clone showed that it contained the entire 3' untranslated region and 30% of the coding sequence for a rat metallothionein. The sequence is remarkably homologous with the mouse metallothionein-I gene.
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D003001:Cloning, Molecular; D003300:Copper / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008667:Metalloproteins / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains
D008667:Metalloproteins; D012313:RNA; D003300:Copper; D004247:DNA; D008668:Metallothionein