Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-Pedersen (TP1-FeSV).
A new feline sarcoma virus designated Theilen-Pedersen (TP1-FeSV) has been isolated from a spontaneous, slowly growing fibrosarcoma of a domestic short-haired 4-year-old castrated cat. The virus codes for a gag-onc fusion protein of 83,000 molecular weight phosphorylated in vivo at serine, threonine, and tyrosine residues. Cells transformed in vitro with TP1-FeSV exhibit five- to 10-fold elevated levels of phosphotyrosine over FeLV-infected cells. The gag-onc polyprotein has associated with it a tyrosyl protein kinase activity which in vitro results in autophosphorylation of the molecule at tyrosine residues. The fusion protein cannot be labeled metabolically with [3H]glucosamine and tunicamycin has no effect on the electrophoretic mobility of the in vivo [32P]orthophosphate-labeled fusion protein. The fusion protein, in common with the gag precursor Pr65gag, can be metabolically labeled with palmitic acid.
D000215:Acylation; D000818:Animals; D002415:Cats; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D015683:Gene Products, gag; D009857:Oncogenes; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000235:genetics; D012190:Retroviridae / Q000302:isolation & purification*; D005253:Sarcoma Viruses, Feline / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis*; D014779:Virus Replication
D015683:Gene Products, gag; D014764:Viral Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D011494:Protein Kinases