[Simple procedures for the determination of hemoglobin F and hemoglobin A2: radial immunodiffusion and column chromatography with commercial kits].
Two commercially available laboratory kits for the determination of HbF and HbA2 with radial immunodiffusion and chromatography in microcolumns have been compared with standard methods. The two kits can be recommended for diagnostic routine laboratories. They are easy to perform and the results were found to be in excellent agreement with those of the alkali denaturation method and electrophoresis with elution of HbA2. The two kits are time-saving, although the reagents and other materials are more expensive.
D002845:Chromatography; D004586:Electrophoresis; D005319:Fetal Hemoglobin / Q000032:analysis*; D006441:Hemoglobin A / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D006452:Hemoglobinometry / Q000379:methods*; D006801:Humans; D005779:Immunodiffusion; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D011933:Reagent Kits, Diagnostic; D006441:Hemoglobin A; D006443:Hemoglobin A2; D005319:Fetal Hemoglobin
Einfache Verfahren zur Bestimmung von Hämoglobin F und Hämoglobin A2: radiale Immundiffusion und Säulenchromatographie mit käuflichen Kits.