A method for processing light microscopy sections for oriented ultramicrotomy.
A simple technique is described for processing optical microscopy sections attached to glass slides for ultramicrotomy in any desired plane. A silicone rubber mold with a central orifice is clamped onto the slide so that the orifice overlies the section. Routine processing and embedding procedures are carried out in the well formed by the orifice.
D006801:Humans; D008854:Microscopy, Electron; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D012867:Skin / Q000648:ultrastructure; D013194:Staining and Labeling