Enzyme induction by daunorubicin in neonatal heart cells in culture.
The effect of the antineoplastic agent daunorubicin on beating heart cells from neonatal rats was investigated with respect to cell damage and induction of enzymes possibly involved in drug metabolism. Of the enzymes assayed DT-diaphorase and glutathione-S-transferase showed a two-to-four fold increase in activity: higher concentrations of daunorubicin inactivated glutathione-S-transferase. Daunorubicin toxicity increased in the presence of dicoumarol, a specific inhibitor of DT-diaphorase. These results indicate that both DT-diaphorase and glutathione-S-transferase may be involved in the metabolism of daunorubicin.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003630:Daunorubicin / Q000494:pharmacology*; D001728:Dicumarol / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000096:biosynthesis*; D009206:Myocardium / Q000201:enzymology*; D016660:NAD(P)H Dehydrogenase (Quinone); D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D011808:Quinone Reductases / Q000096:biosynthesis*; D051381:Rats
D001728:Dicumarol; D009247:NADH, NADPH Oxidoreductases; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D005982:Glutathione Transferase; D003630:Daunorubicin