Synthesis of adult-type hemoglobin in human erythremia cell line.
KMOE -2/05 cells, derived from a patient with acute erythremia, became benzidine-positive after the addition of cytosine arabinoside (CA). Radioimmunoassays using antihuman hemoglobin antibodies revealed an elevated amount of hemoglobin in the CA-exposed cells over that in the control cells (without CA). Isoelectric focusing of the CA-exposed cell lysate formed benzidine-positive foci in the positions of human adult Hb (HbA) and human fetal Hb (HbF). To determine the types of globin synthesized in the CA-exposed cells, globin chains internally labeled with [3H] leucine were purified by carboxy-methyl (CM)-Sephadex column chromatography, immunoadsorption by Sepharose-coupled antihuman Hb antibodies and Sephadex G-100. The labeled globin chains were finally separated by CM-cellulose chromatography in urea. Two distinct peaks of radioactivity were shown in exactly the same fractions as carrier human globin alpha- and beta-chains. These observations indicate that these KMOE -2/05 cells synthesize HbA.
D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology
D006454:Hemoglobins; D008937:Mitomycins; D003561:Cytarabine; D016685:Mitomycin; D005319:Fetal Hemoglobin; D003630:Daunorubicin