An unusual transposon encoding heat shock inducible and developmentally regulated transcripts in Dictyostelium.
We have isolated and characterized a transposable element from Dictyostelium discoideum denoted Tdd-1. There are approximately 50 complete copies of the element per haploid genome and approximately 100-150 partial elements. Southern blots of DNA from different Dictyostelium discoideum strains show that Tdd-1 is a mobile element. Tdd-1 is 4.9 kb long, with 313 bp inverted repeats. These repeats lie near the termini, but unlike other transposable elements one end of Tdd-1 extends 36 bp past the repeat and the other extends 1 bp. Tdd-1 encodes a series of developmentally regulated transcripts, all with the same polarity, that increase dramatically in abundance after approximately 10 hr of development. We have also identified a heat shock inducible transcript of the opposite polarity.
D000818:Animals; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D005800:Genes, Fungal; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes