[Isolation of reverse transcriptase from the avian myeloblastosis virus in preparative quantities].
Inverse transcriptase of bird myeloblastosis virus is a unique instrument for artificial synthesis of structural genes of viruses, plants, animals. Methods for the virus production in preparative amounts are developed due to selection of the corresponding line of chickens, conditions of their maintenance, diet infection methods and myeloblastosis diagnostics. Main demands to the inverse transcriptase preparations (their high activity, absence of nuclease impurities, high concentration of the enzyme preparation solutions and their stability in storage) are ensured by zonal centrifugation purification of the virus in a sucrose density gradient, described methods of inverse transcriptase isolation and purification as well as conditions of its storage.
D000818:Animals; D001353:Avian Leukosis / Q000175:diagnosis; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D001358:Avian Sarcoma Viruses / Q000201:enzymology; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D004926:Escherichia coli / Q000201:enzymology; D007942:Leukemia, Experimental / Q000382:microbiology; D051379:Mice; D008828:Microbiological Techniques; D010766:Phosphorylation; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D011925:Rauscher Virus / Q000201:enzymology
D012194:RNA-Directed DNA Polymerase
Vydelenie obratnoĭ transkriptazy virusa mieloblastoza ptits v preparativnykh kolichestvakh.