A modified tetrazolium reaction for identifying malignant cells from gastric and colonic cancer.
When non-malignant cells were reacted for glucose-6-phosphate dehydrogenase activity, with neotetrazolium chloride as the indicator of the activity, oxygen competed with the neotetrazolium and nullified the reaction. In contrast, about 30% of the activity was retained in malignant cells, in sections and in smears, from cancer of the stomach or colon. This could provide the basis of a qualitative (black-or-white) functional test for distinguishing malignant cells in these conditions.
D003110:Colonic Neoplasms / Q000201:enzymology*; D005753:Gastric Mucosa / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013274:Stomach Neoplasms / Q000201:enzymology*; D013778:Tetrazolium Salts
D013778:Tetrazolium Salts; C011314:neotetrazolium; D005954:Glucosephosphate Dehydrogenase; D009584:Nitrogen; D010100:Oxygen