Induced genetic deficiency of the nucleolar organizer in rat kangaroo cells (PTK1) by ultraviolet laser microirradiation.
An ultraviolet laser microbeam was used to irradiate one of the two nucleolar organizer regions of PTK1 cells in early prophase. The directed nucleolar deficiency induced by ultraviolet laser irradiation was maintained in the daughter cells through subsequent cell generations. However, the frequent occurrence of spontaneous cell fusion in low density cells following the cloning procedure facilitated a recovery of cells to two or more nucleoli.
D000818:Animals; D002460:Cell Line; D007668:Kidney; D007834:Lasers / Q000009:adverse effects*; D007614:Macropodidae; D009697:Nucleolus Organizer Region