Cerebrovascular endothelial cell culture: metabolism and synthesis of 5-hydroxytryptamine.
The presence of 5-hydroxytryptamine was investigated in cultured and propagated cerebrovascular endothelium using immunohistochemistry and high pressure liquid chromatography. These studies demonstrate that the endothelium has the ability to take up and metabolize 5-hydroxytryptamine as well as to synthesize this amine from its precursor L-tryptophan, thus providing evidence for extraneural synthesis of 5-hydroxytryptamine in the central nervous system.
D000818:Animals; D001812:Blood-Brain Barrier; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D002851:Chromatography, High Pressure Liquid; D004727:Endothelium / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007700:Kinetics; D012701:Serotonin / Q000096:biosynthesis / Q000378:metabolism*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid