Localization of serotonin and dopamine in the brown adipose tissue of the rat and their variations during cold exposure.
The variations of several biogenic amines in brown adipose tissue (BAT) during cold exposure were studied and their localization investigated with histological methods. The study of serotonin and its metabolite 5-HIAA suggests that BAT serotonin is mobilized during acute and chronic cold exposure. This amine was found to be principally stored, together with histamine, in mast cells. The mast cell number in BAT was doubled during cold adaptation, as was the histamine content of the tissue. Using radio-enzymatic assay and high pressure liquid chromatography, only small amounts of dopamine were found in BAT. Since no specific dopamine-storing structure was detected (for example SIF cells), this low amount of dopamine is probably the precursor pool for noradrenaline synthesis and is most likely stored in the noradrenergic innervation of the tissue. BAT is known to be sensitive to both exogenous serotonin and exogenous dopamine; according to our results serotonin could play a role in BAT regulation while the role of dopamine remains hypothetical.
D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D003080:Cold Temperature; D004298:Dopamine / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism; D006651:Histocytochemistry; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000502:physiology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006632:Histamine; D004298:Dopamine; D009638:Norepinephrine