Correlation between structure of polyoxotungstates and their inhibitory activity on polymerases.
The 21-tungsto-9-antimonate (TA, HPA 23), a polyoxotungstate, has shown a significant antiviral activity in vivo and in vitro. It inhibits viral and bacterial DNA polymerases. In this paper, several compounds of two polyoxotungstic families, tungstoantimonates and tungstoarsenates, have been used to specify the mechanism of polymerase inhibition. It has been demonstrated that the inhibitory activity of polyoxotungstates is not related to the occupation of their coordinates sites by cations, nor to the nature of these bound cations. Kinetic studies and binding assays have shown that polyoxotungstates bind to the polymerases in competition with the nucleic acid template. This result seems to be related to their polyanionic nature. Furthermore, the size and charge of these compounds may play a prominent part in their affinity for the polymerases.
D000965:Antimony / Q000494:pharmacology*; D001149:Arsenates / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000201:enzymology; D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; D013379:Substrate Specificity; D014414:Tungsten / Q000494:pharmacology*; D017973:Tungsten Compounds
D001149:Arsenates; D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; D017973:Tungsten Compounds; C039606:tungstoarsenate; C009613:ammonium tungsten antimonate hydroxide oxide; D000965:Antimony; D012321:DNA-Directed RNA Polymerases; D014414:Tungsten