Monoclonal antibodies against beta-galactoside-binding lectin of chick embryo recognizes conformational change of the antigen.
Monoclonal antibodies against chick embryonic beta-galactoside-binding lectin were obtained. One of the monoclonal antibodies was ineffective in Western blotting and seemed to be unable to bind the SDS-denatured lectin. When the native lectin was dotted on a nitrocellulose filter and subjected to denaturation by treatment with SDS, urea or heat, binding of this antibody no longer occurred, though other monoclonal antibodies bound normally. This antibody seems to have been raised against an epitope which is destroyed upon denaturation.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002642:Chick Embryo; D000939:Epitopes / Q000032:analysis*; D037161:Galectins; D006388:Hemagglutinins / Q000032:analysis* / Q000276:immunology; D011487:Protein Conformation; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D037161:Galectins; D006388:Hemagglutinins; D012967:Sodium Dodecyl Sulfate; D014508:Urea