A variant form of beta-actin in a mutant of KB cells resistant to cytochalasin B.
Lines of KB cells resistant to cytochalasin B have been isolated and characterized. The mutant Cyt 1 exhibits increased cross-resistance to cytochalasin E. Cyt 1 cells bind less cytochalasin B than parental KB cells. Two-dimensional gel analysis shows that Cyt 1 cells carry an alteration in beta-actin. This is further confirmed by one-dimensional peptide analysis. The altered beta-actin (beta'-actin) is synthesized when poly(A)+ RNA from Cyt 1 cells is translated in a reticulocyte-cell-free translation system. These results suggest that the primary site of action of cytochalasin B on cellular motility processes is beta-actin.
D000199:Actins / Q000235:genetics* / Q000378:metabolism; D002277:Carcinoma; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003571:Cytochalasin B / Q000378:metabolism / Q000633:toxicity*; D004351:Drug Resistance; D014644:Genetic Variation; D006801:Humans; D007700:Kinetics; D009062:Mouth Neoplasms; D009154:Mutation; D011061:Poly A / Q000235:genetics; D011485:Protein Binding; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger
D000199:Actins; D012333:RNA, Messenger; D011061:Poly A; D003571:Cytochalasin B; D012313:RNA