Purification of human lymphoblastoid cell-derived interferon-alpha by controlled-pore glass bead adsorption chromatography and molecular sieving.
Human lymphoblastoid cell-derived interferon-alpha has been purified by a combination of chromatography on controlled-pore glass 350, gel filtration on Ultrogel AcA 54 and hydrophobic chromatography on Phenyl-Sepharose CL-4B. Specific activities of greater than 10(7) units/mg have been obtained. Controlled-Pore Glass has the advantages of accommodating large volumes of induced medium in an "in-line" process giving high recoveries of interferon and removing most of any contaminating DNA.
D002478:Cells, Cultured; D002850:Chromatography, Gel / Q000379:methods*; D004247:DNA; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007372:Interferons / Q000302:isolation & purification*; D008214:Lymphocytes; D018139:Simplexvirus / Q000235:genetics
D004279:DNA, Viral; D004247:DNA; D007372:Interferons