Synthesis of human cytomegalovirus-specified RNA and protein in interferon-treated cells at early times after infection.
In human fibroblast cells treated with interferon, cytomegalovirus-specified immediate early RNA was found associated with the polyribosomes at concentrations and size classes similar to the virus RNA found in non-treated cells, Interferon treatment inhibited the translation of the immediate early virus mRNA; the relative rate of virus-specified immediate early protein and antigen synthesis decreased with increasing concentrations of interferon. In addition, the relative amount of virus-specified RNA associated with the polyribosomes at early times after infection was significantly reduced by treatment of the cells with interferon. Inhibition of infectious virus production in interferon-treated cells was primarily due to inhibition of immediate early virus protein synthesis and secondarily to suppression of early virus RNA synthesis. The role of the virus-specified immediate early proteins in regulating subsequent virus gene expression is discussed.
D002460:Cell Line; D003587:Cytomegalovirus / Q000254:growth & development / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D011132:Polyribosomes / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D014764:Viral Proteins / Q000096:biosynthesis*
D012367:RNA, Viral; D014764:Viral Proteins; D007372:Interferons