Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase.
A series of cDNA clones corresponding to 1494 bases of rat muscle creatine kinase mRNA has been isolated and characterized. The identity of these clones has been confirmed by DNA sequence analysis and by comparison of the predicted amino acid sequence with that determined for the purified protein. The cDNA sequence accounts for the entire coding sequence of the creatine kinase protein in addition to the complete 3' untranslated region and 68 bases of 5' noncoding region. Sequences corresponding to the active site region of the protein, the initiation codon, the termination codon, and poly(A) addition signal have been identified.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005796:Genes; D009132:Muscles / Q000201:enzymology*; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D003402:Creatine Kinase; D004262:DNA Restriction Enzymes