[Properties of cloned promoters].
Strong early bacteriophage T7 promoters A2 and A3 and also A2 and lac UV5 promoters with altered segments downstream the initiation of RNA start point were cloned using specially constructed plasmid vectors pBRS188 and PBRS240. The relative signal strengths of these promoters in vivo and in vitro were evaluated and the kinetic parameters of their interaction with RNA polymerase were determined. It has been shown that the nucleotide sequence of the transcribed region plays a significant role in specific promoter-RNA polymerase interaction and that the rate-limiting step of RNA synthesis initiation is different for various promoters.
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D007700:Kinetics; D007763:Lac Operon; D010957:Plasmids; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013604:T-Phages / Q000235:genetics; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes
Izuchenie svoĭstv klonirovannykh promotorov.