Substance P-like immunoreactivity in the pulp-dentine zone of human molar teeth demonstrated by indirect immunofluorescence.
Freshly-extracted human third molars were fixed in Zamboni fixative, demineralized with the mixture of EDTA and the fixative; substance P-like immunoreactivity (SPLI) was revealed by the indirect immunofluorescence technique of Coons. Substance P (SP) was observed in the pulp-dentine zone and the dental pulp. Some of SP-containing fibres ended at the odontoblast layer and did not reach the predentine; others terminated at the predentine surface or penetrated into the predentine. In the predentine, some of SP fibres accompanied odontoblast processes and ended near the mineralized dentine; others changed course transversely at various levels.
D003782:Dental Pulp / Q000032:analysis*; D003804:Dentin / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008963:Molar / Q000032:analysis / Q000294:innervation; D013373:Substance P / Q000032:analysis*
D013373:Substance P