Detection of anti-RNA antibodies in systemic lupus erythematosus by ELISA using nylon as solid support.
A highly sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the detection of anti-RNA antibodies is described. The assay procedure involves adsorption of total cellular RNA on nylon beads which could be conveniently stored for a considerable period of time without loss in antigenicity. Sixty-four percent of systemic lupus erythematosus (SLE) sera were positive for anti-RNA antibodies with fluorogenic substrate against 48% with colorigenic substrate.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009757:Nylons; D012313:RNA / Q000276:immunology*
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D009757:Nylons; D012313:RNA