title: Antiidiotype stimulation of antigen-specific antigen-independent antibody responses in vitro. I. Evidence for stimulation of helper T lymphocyte function.
abstract: Antibodies specific for the idiotypes of B10 anti-(T,G)-A-L antibodies (anti-Id) induced in vivo (T,G)-A-L-primed lymphocytes to secrete anti-(T,G)-A-L antibodies in vitro in the absence of antigen. Responses to anti-Id were quantitatively and qualitatively similar to responses to antigen. Responses were specific in that: (a) only lymphocytes primed in vivo with (T,G)-A-L (but not other antigens or unprimed cells) were inducible; (b) only anti-Id (but not a variety of control antibodies) induced responses; and (c) only anti-(T,G)-A-L antibodies were secreted. Antigen-primed T lymphocytes mixed with unprimed B plus accessory cells also responded to anti-Id and the phenotype of the responding T cells was Lyt-1+, Lyt-2-. Supernatants obtained from antigen-primed T cells incubated for 3 d with anti-Id (but not when incubated with controls) provided help to unprimed B plus accessory cells in the presence of anti-Id, thus providing direct evidence for induction of T lymphocyte helper function by anti-Id. In contrast to responses to (T,G)-A-L, responses to anti-Id did not appear to be regulated by H-2-linked Ir genes. The system described is a powerful tool for delineation of the mechanisms whereby antiidiotype antibodies affect lymphocyte function.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000955:Antigens, T-Independent / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000502:physiology; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000955:Antigens, T-Independent; D000939:Epitopes; D006183:H-2 Antigens; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D007376:Interleukin-2; D010455:Peptides; C021759:(T,G)-A-L