title: Deoxyribonucleic acid breaks produced by 4'-(9-acridinylamino)methanesulfon-m-anisidide and copper.
abstract: We have demonstrated that 4'-(9-acridinyl-amino)methanesulfon-m-anisidide (mAMSA), in the presence of Cu(II) ion, causes the breakage of plasmid pDPT275 and pBR322 superhelical form I DNA. In neutral pH, the degradative product was nicked, relaxed form II DNA, resulting from single-stranded DNA breakage. The extent of DNA breakage was both mAMSA concentration and Cu(II) concentration dependent. DNA breakage increased with increasing time of drug treatment. The mAMSA-Cu(II)-induced DNA breakage varied with pH values and also with the nature of the buffer systems. In both Tris-HCl and borate buffers the extent of DNA breakage increased with increasing pH. In Tris-HCl buffer (pH 7-9), only single-strand breaks were obtained, whereas in borate buffer (pH 9-10.5), linear form III DNA was obtained. At equivalent pH, the optimum buffer was borate. No breakage was observed at pH values below 6. The interaction of Cu(II) with mAMSA was examined by using absorption and fluorescence spectroscopies. Interaction of Cu(II) with mAMSA was characterized by a decrease in the absorption at 435 and 420 nm with a simultaneous increase at 330 nm. A highly fluorescent product was obtained upon reacting mAMSA with Cu(II), with an emission spectrum (excitation at 400 nm) showing a doublet at 430 and 450 nm and a shoulder around 480 nm. The spectral changes are also dependent similarly on the pH and the nature of buffer. Other divalent metal ions such as Co(II), Cd(II), Ni(II), and Zn(II) do not induce DNA breakage or spectral changes. The oAMSA isomer, which has no antitumor activity, is less effective in inducing DNA breakage than the mAMSA.
mesh_terms: D000609:Aminoacridines / Q000494:pharmacology*; D000677:Amsacrine; D001761:Bleomycin / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000302:isolation & purification; D004278:DNA, Superhelical / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000494:pharmacology*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000187:drug effects*; D013053:Spectrophotometry
chemical_list: D000609:Aminoacridines; D004278:DNA, Superhelical; D007364:Intercalating Agents; D000677:Amsacrine; D001761:Bleomycin; D003300:Copper; D004247:DNA