title: Decreased production of RNA-streptolysin S in streptococci devoid of extracellular RNase activity.
abstract: In cultures of certain streptococcal strains, RNA added as a carrier for streptolysin S (SLS) was hardly degraded, owing to deficiency of extracellular RNase activity. Production of RNA-SLS into culture supernatant was markedly reduced in the RNase-deficient streptococci. Even in these RNase-less strains, guanylic-acid rich oligonucleotides, polyG or trypan blue effectively induced SLS production, as in RNase-positive cells. These results demonstrate involvement of the streptococcal nuclease in manifestation of SLS-inducing effect of exogenous RNA. Additional data indicated that cellular growth was promoted by supplementation of RNA, in the nuclease-producing streptococci.
mesh_terms: D001426:Bacterial Proteins; D007700:Kinetics; D009154:Mutation; D012329:RNA, Bacterial / Q000235:genetics*; D012260:Ribonucleases / Q000378:metabolism*; D013045:Species Specificity; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics; D013301:Streptolysins / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D013301:Streptolysins; C026050:streptolysin S; D012260:Ribonucleases