Studies of chloroplast thylakoid proteins using monoclonal antibodies.
Several monoclonal antibodies have been produced against partially purified photosystem I reaction center complexes isolated from spinach chloroplasts. One of the clones was shown to be highly specific for the 28,000 and 27,000 dalton subunits of purified light harvesting chlorophyll a/b binding complex. Studies with thylakoids suggest at least a portion of the light harvesting chlorophyll a/b binding protein molecules are exposed on a normally inaccessible surface of the membrane.
D000911:Antibodies, Monoclonal; D002734:Chlorophyll / Q000032:analysis; D060365:Chloroplast Proteins; D002736:Chloroplasts / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D045342:Light-Harvesting Protein Complexes; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D009940:Organoids / Q000032:analysis; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000032:analysis; D010944:Plants / Q000032:analysis
D000911:Antibodies, Monoclonal; D060365:Chloroplast Proteins; D000939:Epitopes; D045342:Light-Harvesting Protein Complexes; D046911:Macromolecular Substances; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; C042085:thylakoid membrane phosphoprotein, plant; C021261:thylakoid polypeptides; D002734:Chlorophyll