[Histochemical method for determining phenol oxidase in spontaneous rosette-forming cells of human blood].
Using preparations of leucocytes and lymphocytes the reaction of phenoloxidase containing cells (POZ) during the spontaneous rosette formation was investigated. Beside the hitherto known cells forms 3 new types of PO-positive lymphocytes were found. It was established that the large methanol resistent POZ appear in the preparations of lymphocytes, too. Regarding their composition, isolated lymphocytes represent therefore a changing population of cells. Although PO-positive cells take part in rosette forming, the results gave no clear-cut evidence whether T-lymphocytes also contain PO. The application of the PO-reaction opens new aspects in the haematological and immunological diagnostics.
D004156:Catechol Oxidase / Q000097:blood*; D005557:Formaldehyde; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007962:Leukocytes / Q000201:enzymology / Q000276:immunology; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology; D000432:Methanol; D012397:Rosette Formation; D013194:Staining and Labeling
D005557:Formaldehyde; D004156:Catechol Oxidase; D000432:Methanol
Untersuchungen mit dem histochemischen Nachweis für Phenoloxidase an spontanrosettenbildenden Zellen des menschlichen Blutes.