Fixation and immunoperoxidase staining of oligopeptides after isoelectric focusing in thin polyacrylamide slab gels.
In this paper a new method for immunological detection of small peptides after isoelectric focusing in thin (200 micron) polyacrylamide slab gels is presented. The peptides are immobilized immediately after focusing by pressing a sheet of glutaraldehyde-impregnated filter paper onto the gel. By this procedure, the gel adheres to the paper and this press-blot can be stained using immunoperoxidase staining procedures. Using the two-step peroxidase conjugate and the three-step peroxidase-antiperoxidase method, several oligopeptides could be visualized after focusing. The detection limit of this method appears to be in the nanogram range.
D000180:Acrylic Resins; D007124:Immunoenzyme Techniques; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D009842:Oligopeptides / Q000032:analysis*; D013194:Staining and Labeling
D000180:Acrylic Resins; D007457:Iodine Radioisotopes; D009842:Oligopeptides; C016680:polyacrylamide gels