[Increase in cell metabolism in normal, diploid human glial cells in stationary cell cultures induced by meclofenoxate].
Quantitative biochemical studies were undertaken in order to examine the influence of the accumulation of lipofuscin in secondary lysosomes on cell metabolic activities of normal diploid human glia cells in a stationary cell culture system. Glia cells accumulate lipofuscin as a function of the duration of the stationary cultivation in vitro. The accumulation of lipofuscin can be decreased by the long-term treatment with the pharmacon meclofenoxate (centrophenoxine, Helfergin). Concomitant with the reduction of the accumulated lipofuscin, meclofenoxate-treated glia cells show enhanced rates of RNA synthesis, protein synthesis and glucose uptake. Most likely, in meclofenoxate-treated normal diploid human glia cells in vitro, the utilisation of glucose is shifted from glycolysis to the pentose phosphate pathway. The data suggest that the meclofenoxate-induced reduction of lipofuscin accumulation has a positive effect on cell metabolic functions and causes a delay of the cellular aging of the human glia cells in vitro.
D004171:Diploidy; D005947:Glucose / Q000378:metabolism; D006016:Glycolates / Q000494:pharmacology*; D006801:Humans; D008062:Lipofuscin / Q000378:metabolism; D002504:Meclofenoxate / Q000378:metabolism / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009457:Neuroglia / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis
D006016:Glycolates; D008062:Lipofuscin; D009419:Nerve Tissue Proteins; D012313:RNA; D002504:Meclofenoxate; D005947:Glucose
Erhöhung von Zellstoffwechselleistungen in normalen, diploiden, menschlichen Glia-Zellen in stationären Zellkulturen induziert durch Meclofenoxat.