Detection of bovine pancreatic trypsin inhibitor (bPTI) in bovine and ovine pituitaries, bovine brain, and adrenal medulla. A specific radioimmunoassay for bPTI.
An antiserum against bovine pancreatic trypsin inhibitor (bPTI) has been raised in rabbits. The antiserum does not cross-react with known pituitary hormones and other proteins. A specific radioimmunoassay for bPTI has been developed. The ED50 is about 250 fmol per assay tube and the sensitivity is reliable to 15 fmol per tube. Immunoreactivity could be detected in bovine and sheep pituitaries, bovine brain, and adrenal medulla, but not in human, pig, and rat pituitaries. In the bovine pituitary the immunoreactivity is restricted to the posterior lobe.
D000313:Adrenal Medulla / Q000032:analysis*; D000818:Animals; D007611:Aprotinin / Q000032:analysis*; D001923:Brain Chemistry; D002417:Cattle; D003429:Cross Reactions; D009928:Organ Specificity; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep; D013045:Species Specificity
D007611:Aprotinin