[Inhibition of cyclic nucleotide phosphodiesterase from the rod outer segments of the frog retina by 3-hydroxypyridine].
The influence of 8 analogues of 3-hydroxypyridine upon the phosphodiesterase of rod outer segments of frog retinae has been investigated. It has been shown that the analogues of 3-hydroxypyridine inhibit the enzeme reversely and noncompetitively in case of hydrolysis towards the cAMP and cGMP. The natural analogues of 3-hydroxypyridine (pyridoxol, pyridoxale, pyridoxale-phosphate) do not exert the inhibiting effect. It is suggested that the inhibition of phosphodiesterase from rod outer segments of retinae is caused by the interaction of 3-hydroxypyridines with the hydrophobic microenvironment of the active site of the enzyme.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000037:antagonists & inhibitors*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D066298:In Vitro Techniques; D010726:Phosphodiesterase Inhibitors; D010727:Phosphoric Diester Hydrolases; D010786:Photoreceptor Cells / Q000201:enzymology*; D011725:Pyridines / Q000494:pharmacology*; D011895:Rana ridibunda; D012374:Rod Cell Outer Segment / Q000187:drug effects / Q000201:enzymology*; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D010726:Phosphodiesterase Inhibitors; D011725:Pyridines; C023500:3-hydroxypyridine; D013806:Theophylline; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D015056:1-Methyl-3-isobutylxanthine
Ingibirovanie fosfodiésterazy tsiklicheskikh nukleotidov iz naruzhnykh segmentov palochek setchatki liagushki 3-oksipiridinami.