Characterization of cellular attachment and spreading molecules at liquid-liquid interfaces.
Cells are grown at a liquid-liquid interface (ultrafiltrate of growth medium--heptacosafluorotributylamine). Following attachment and spreading an intermediate-density, isotonic solution of metrizamide-Ficoll is introduced. Cells are detached by centrifugation at 150,000g X 45 min. Molecules deposited at the interface are removed and examined by gel electrophoresis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals 20 protein bands ranging in molecular weight from 30,000 to 200,000. Most bands have molecular weights less than 120,000. The substrate-attached material contains approximately 2.8 pg of protein per attached cell. Substrate-attached molecules can be removed without detergent denaturation for additional studies.
D000818:Animals; D002462:Cell Membrane; D002477:Cells / Q000166:cytology*; D002478:Cells, Cultured; D002498:Centrifugation; D002642:Chick Embryo; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000166:cytology; D012834:Silver; D013194:Staining and Labeling; D013499:Surface Properties
D003470:Culture Media; D012834:Silver