title: Denaturation of RNA and DNA in situ induced by acridine orange.
abstract: The products of interaction of acridine orange (AO) with single-stranded (ss) nucleic acids are precipitates which exhibit red luminescence. Titration of rRNA or thymus DNA with AO results in formation of such products suggesting that the dye, per se, denatures double-stranded (ds) sections of these biopolymers. This transition, measured as the increase of red luminescence, a concomitant decrease of green fluorescence, and followed by an increase of light scatter of the AO-nucleic acid complexes, is cooperative and at 0.15 N NaCl occurs at 4-20 and 10-50 microM range of AO concentration for rRNA and DNA, respectively. The changes in stainability of nucleic acids in situ, in permealized cells, occur at higher AO concentration. Thus, the transition of RNA in situ is biphasic and seen at 20-120 microM AO. In the presence of EDTA, however, the change is monophasic and shifted to the 10-30 microM range of AO concentration. The change in stainability of DNA also shows two phases: one at 30-60 microM and another at 70-120 microM of AO. Extraction of basic proteins with 0.08 N HCl shifts the transition of DNA to the 30-60 microM AO concentration and makes it monophasic. The observed differences in denaturability of RNA vs DNA explain the specificity of AO in differential staining of these bipolymers in histochemical reactions. In living cells the products of interaction of AO with nucleic acids are detected by electron microscopy. In the cytoplasm of interphase cells the formation of dense precipitates within ribosomes and polysomes, simultaneous with a specific retraction of ribosome-polysome complexes from the periphery of the cell to the nucleus is evident. The latter suggests higher order organization of these particles involving their association with each other or with the nucleus via polyanionic macromolecules which collapse upon binding with AO. The DNA in heterochromatin is more sensitive to AO-induced denaturation, as evidenced by the fact that the dense complexes are formed preferentially in the regions of condensed chromatin of the interphase nucleus, or in metaphase chromosomes.
mesh_terms: D000165:Acridine Orange / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004247:DNA; D006570:Heterochromatin / Q000032:analysis; D007399:Interphase; D007942:Leukemia, Experimental; D008677:Metaphase; D051379:Mice; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation / Q000187:drug effects*; D012313:RNA; D012335:RNA, Ribosomal; D012270:Ribosomes / Q000032:analysis; D013194:Staining and Labeling
chemical_list: D006570:Heterochromatin; D012335:RNA, Ribosomal; D012313:RNA; D004247:DNA; D000165:Acridine Orange