title: The chloroacetate esterase reaction for mast cells in dermatopathology: a comparison with metachromatic staining methods.
abstract: This paper compares standard metachromatic methods with Leder's chloroacetate esterase reaction on mast cells in paraffin wax-embedded tissue of urticaria pigmentosa and a variety of inflammatory and benign neoplastic cutaneous conditions. Our conclusions are that Leder's method allows easier identification of mast cells and can be a useful adjunct to conventional metachromatic methods. The technique could be conveniently adopted in routine dermatopathology for mast cell identification.
mesh_terms: D002265:Carboxylic Ester Hydrolases; D006652:Histological Techniques; D006801:Humans; D008407:Mast Cells; D012871:Skin Diseases / Q000175:diagnosis*; D013194:Staining and Labeling; D014582:Urticaria Pigmentosa / Q000175:diagnosis*
chemical_list: D002265:Carboxylic Ester Hydrolases; C019873:chloroacetate esterase