Flexible porous discs by radiation polymerization method for enzyme immunoassay of alpha-fetoprotein.
New porous discs for enzyme immunoassay of alpha-fetoprotein (AFP) have been prepared by radiation polymerization of various monomers at low temperature. The activity (optical density) of immobilized anti-AFP disc varied with irradiation temperature, anti-AFP concentration, and the hydrophilicity of monomer, in which copolymerization of hydrophilic 2-hydroxyethyl methacrylate and hydrophobic monomers at low temperature below 0 degrees C gave a high activity. The immobilized anti-AFP disc was flexible and had a porous structure. The immobilized anti-AFP discs in dry state are convenient for storage. The minimum serum volume in the assay was 5 microliter. A good correlation (r = 0.98) was noted between AFP concentrations measured by ratio immunoassay and enzyme immunoassay with immobilized anti-AFP disc. The mean recovery of AFP (16-80 ng) added to serum was 95-110%.
D000818:Animals; D000906:Antibodies; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D011108:Polymers; D011817:Rabbits; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D011108:Polymers; D000509:alpha-Fetoproteins