Fetal neural tube defects detected by rocket-on-line immunoelectrophoresis of amniotic fluids.
Immunization of rabbits with human fetal brain membranes evoked antisera with reactivity against D-2 protein from brain and cerebrospinal fluid. In amniotic fluids from pregnancies with fetal neural tube defects the presence of D2-protein could be demonstrated by rocket-on-line immunoelectrophoresis. In a prospective study on 72 amniotic fluids with increased alphafetoprotein concentration neural tube defects were correctly predicted in 26 pregnancies. One false-negative and four false-positive pregnancies were encountered. D2-protein determination may thus have a role in antenatal diagnosis of neural tube malformations as a supplement to alphafetoprotein analysis.
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry; D002555:Cerebrospinal Fluid / Q000032:analysis; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D009436:Neural Tube Defects / Q000175:diagnosis*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D000509:alpha-Fetoproteins / Q000032:analysis
D011506:Proteins; D000509:alpha-Fetoproteins