Biological activity of canine intestinal cholecystokinin-58.
The biological activity of a recently characterized cholecystokinin molecule, canine CCK-58, has been tested using perifused canine gallbladder strips and isolated mouse pancreatic acini. It has been shown that canine CCK-58, purified by affinity chromatography and molecular sieve chromatography, is biologically as potent as canine CCK-39,33 (component II) and synthetic CCK-8. The biological responses were inhibited by dibutyryl cGMP, a known CCK antagonist. CCK-58 is one of the main large molecular forms of CCK in the intestine mucosa. Whether CCK-58 is only an intracellular precursor of other CCK molecules or an important circulating hormone cannot be determined from the data currently available.
D000109:Acetylcholine / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002766:Cholecystokinin / Q000494:pharmacology*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004285:Dogs; D005704:Gallbladder / Q000502:physiology; D007413:Intestinal Mucosa / Q000502:physiology*; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship
D003995:Dibutyryl Cyclic GMP; C038823:cholecystokinin 58; D002766:Cholecystokinin; D000681:Amylases; D000109:Acetylcholine