title: Retrograde axoplasmic transport of neurosecretory material. An immunocytochemical and electron-microscopic study of transected axons in normal and colchicine-treated rats.
abstract: The axonal transport of neurosecretory material was studied in neurosecretory axons of the supraoptico-posthypophyseal system after in-situ transection of the median eminence. Two hours, 8 h, and 18 h after the lesion, both vasopressin and oxytocin antibodies revealed progressive accumulations of immunoreactive material not only in the proximal but also in the distal stumps of the transected axons. The electron-microscopic examination of these axonal portions revealed that such intense immunopositive labelings could be correlated, in both stumps, to a conspicuous accumulation of neurosecretory granules. It is concluded that, under normal physiological conditions, a significant amount of axoplasmic neurosecretory material is transported in retrograde direction and that such a retrograde transport mainly involves neurosecretory granules.
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D001369:Axons / Q000378:metabolism* / Q000601:surgery / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology*; D006651:Histocytochemistry; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism / Q000648:ultrastructure; D007120:Immunochemistry; D008297:Male; D008473:Median Eminence / Q000601:surgery; D008854:Microscopy, Electron; D009489:Neurosecretion / Q000187:drug effects*; D010121:Oxytocin / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014667:Vasopressins / Q000032:analysis
chemical_list: D014667:Vasopressins; D010121:Oxytocin; D003078:Colchicine