Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization.
Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization at low temperatures was studied. The antibody activity of antibody was not affected by irradiation at an irradiation dose of below 8 MR and low temperatures. Immobilization of peroxidase-labeled anti-rabbit IgG goat IgG, anti-peroxidase, peroxidase, and anti-alpha-fetoprotein was carried out with hydrophilic and hydrophobic monomers. The activity of the immobilized enzyme-labeled antibody membranes varied with the thickness of the membranes and increased with decreasing membrane thickness. The activity of the immobilized antibody particles was varied by particle size. Immobilized anti-alpha-fetoprotein particles and membranes can be used for the assay of alpha-fetoprotein by the antigen-antibody reaction, such as a solid-phase sandwich method with high sensitivity.
D000179:Acrylates / Q000528:radiation effects*; D000906:Antibodies; D004307:Dose-Response Relationship, Radiation; D004800:Enzymes, Immobilized; D006801:Humans; D007124:Immunoenzyme Techniques; D010544:Peroxidases; D000509:alpha-Fetoproteins / Q000276:immunology
D000179:Acrylates; D000906:Antibodies; D004800:Enzymes, Immobilized; D000509:alpha-Fetoproteins; D010544:Peroxidases