title: Demonstration that the temporary sequestering of adventitious iron accounts for the inhibition of microsomal lipid peroxidation by bleomycin A2.
abstract: One of the manifestations of the interaction of bleomycin with the microsomal mixed-function oxidase system is an inhibition of NADPH-dependent, reactive oxygen-mediated lipid peroxidation. The results of this investigation demonstrate that this effect by bleomycin can be attributed to the sequestering of adventitious iron. However, as a result of the microsome-catalyzed, iron-facilitated activation of bleomycin and the concomitant spontaneous decay of the activated bleomycin intermediate, iron can apparently no longer be bound by the bleomycin molecule; consequently, the inhibition of microsomal lipid peroxidation by bleomycin is only temporary.
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism; D003855:Deoxyribose / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D008054:Lipid Peroxides; D001761:Bleomycin; D008315:Malondialdehyde; D003855:Deoxyribose; D003300:Copper; D004492:Edetic Acid; D007501:Iron