Effect of ruthenium red on the Ca2+ and Sr2+ efflux from rat liver mitochondria: influence of nupercaine.
The rate of ruthenium-red-induced Ca2+ efflux depends on the time that the calcium interacts with the mitochondria prior to the addition of the inhibitor. This time-dependency is abolished in the presence of nupercaine; it does not occur in the case of Sr2+ efflux from mitochondria in which the endogenous Ca2+ has been substituted by strontium (strontium-treated mitochondria, STM). Ruthenium red inhibits the respiratory-inhibitor- or uncoupler-induced Sr2+ efflux from STM, but not the Ca2+ efflux from standard mitochondria. The influence of the calcium-induced mitochondrial damage upon the effect of ruthenium red is discussed.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003992:Dibucaine / Q000494:pharmacology*; D007700:Kinetics; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D012428:Ruthenium / Q000494:pharmacology*; D012430:Ruthenium Red / Q000494:pharmacology*; D013324:Strontium / Q000378:metabolism*; D014475:Uncoupling Agents / Q000494:pharmacology
D014475:Uncoupling Agents; D012430:Ruthenium Red; D012428:Ruthenium; D003992:Dibucaine; D002118:Calcium; D013324:Strontium