[Effect of nalidixic acid on the activation of RNA synthesis in outgrowing spores of Bacillus subtilis].
Outgrowth of B. subtilis spores depends on the action of DNA gyrase (comp. Matsuda and Kameyama 1980). Application of nalidixic acid (100 micrograms/ml) to dormant spores of Bacillus subtilis prevents the outgrowth. Application of nalidixic acid (100 micrograms/ml) during the early outgrowth phase (after a 20 min germination period) does not prevent, but only delay spore outgrowth. Germination of spores is not influenced. Nalidixic acid is an effective inhibitor of RNA synthesis in outgrowing spores, whereas vegetative cells are more resistant. Spores can grow out inspite of a remarkably reduced intensity of RNA synthesis. Nalidixic acid particularly inhibits the synthesis of stable RNA, probably that of ribosomal RNA. We suggest that DNA gyrase-catalyzed alterations in DNA structure are involved in the regulation of the gene expressional program of outgrowing B. subtilis spores.
D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D009268:Nalidixic Acid / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D013171:Spores, Bacterial / Q000187:drug effects / Q000378:metabolism
D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D009268:Nalidixic Acid; D004250:DNA Topoisomerases, Type II
Einfluss von Nalidixinsäure auf die Aktivierung der RNA-Synthesen auswachsender Sporen von Bacillus subtilis.