Binding of hormones and neuropeptides by calmodulin.
Calmodulin exhibits high-affinity, calcium-dependent binding of 1 mol/mol of the vasoactive intestinal peptide (VIP), secretin, and either the 42- or 43-residue gastric inhibitory peptide (GIP) with dissociation constants of 0.05-0.14 microM. The affinity of VIP for calmodulin approaches its affinity for the cell-surface VIP receptors. These peptides compete with both smooth muscle myosin light chain kinase and glucagon in calmodulin binding. Calculation of amino acid frequencies for eight calmodulin binding peptides (VIP, GIP, secretin, ACTH, beta-endorphin, substance P, glucagon, and dynorphin [Malencik, D. A., & Anderson, S. R. (1982) Biochemistry 21, 3480]) shows a below-average incidence of glutamyl residues, above-average incidence of glutaminyl residues, and average incidence of both aspartyl and asparaginyl residues. Predictions of structure from sequence suggest that the bound peptides contain strongly basic turns and coils in close association with regions having above-average beta-sheet potential. The temperature dependence of glucagon binding by calmodulin shows that the association is enthalpy driven.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D004399:Dynorphins; D004723:Endorphins / Q000378:metabolism; D005749:Gastric Inhibitory Polypeptide / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006728:Hormones / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012633:Secretin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D013552:Swine; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D001615:beta-Endorphin
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D004723:Endorphins; D006728:Hormones; D012633:Secretin; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D005749:Gastric Inhibitory Polypeptide; D001615:beta-Endorphin; D004399:Dynorphins; D000324:Adrenocorticotropic Hormone; D005934:Glucagon