Localization of two antigenic determinants in histone H4.
Four overlapping synthetic peptides corresponding to the carboxy-terminal region 80-102 of histone H4 were prepared by solid-phase peptide synthesis. Their antigenic activity was analysed by inhibition of the H4-anti-H4 reaction in complement fixation and enzyme-linked immunosorbent assay. One antigenic determinant was localized in residues 88-96 of the H4 molecule. No antigenic activity was found in peptides 80-89 and 97-102. Antibodies induced by peptide 85-102 were found to bind to free H4 in solution but not to chromatin subunits, suggesting a lack of accessibility of the C-terminal region of H4 in nucleosomes. A second epitope was found to be situated in the N-terminal region 1-53 of histone H4.
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000097:blood / Q000276:immunology*; D007106:Immune Sera; D010446:Peptide Fragments / Q000276:immunology
D002843:Chromatin; D000939:Epitopes; D006657:Histones; D007106:Immune Sera; D010446:Peptide Fragments