title: Potentiation of antibody responses by specific IgM: specificity and thymus dependency.
abstract: Modulation of antibody responses induced by IgM directed against the immunogen was investigated. When IgM directed against ox erythrocytes (ORBC) was given together with trinitrophenyl (TNP)-ORBC, the subsequent antibody response to the carrier, ORBC, as well as the response to the hapten, TNP, was potentiated. In contrast, IgG with carrier specificity inhibited both responses. The hapten-specific potentiation was found in both direct and indirect plaques, and was antigen-dose dependent, i.e., no potentiation was found with the lowest antigen doses. The response to 2,4-dinitrophenyl (DNP)-labeled proteins was potentiated by a monoclonal IgM with specificity for the hapten. The effects were observed both in primary and secondary responses. One strict requirement for IgM potentiation to occur was observed. The determinant against which potentiation was achieved had to be physically linked to the determinant against which the IgM was directed, be it hapten or carrier determinants. Thus, irrelevant IgM-antigen complexes were incapable of potentiating the responses. Similar specificity requirements were found for IgG induced suppression of antibody responses. Experiments with nude mice and their euthymic littermates showed that IgM potentiation of antibody production is T-cell dependent. Furthermore, passive transfer of carrier-primed spleen cells together with antigen challenge suggests that IgM potentiation of secondary antibody responses is dependent on specific carrier-primed immune T cells.
mesh_terms: D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism*; D007518:Isoantibodies / Q000096:biosynthesis; D007519:Isoantigens / Q000008:administration & dosage; D007700:Kinetics; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000939:Epitopes; D006241:Haptens; D007075:Immunoglobulin M; D007518:Isoantibodies; D007519:Isoantigens; D014301:Trinitrobenzenes