[Attempt to use monocytes for HLA-DR typing].
Two methods of isolating monocytes from peripheral defibrinized blood are described and compared with each other: 1. Adherence of monocytes on the surface of Petri-dishes and 2. Two-phase technique with centrifugation over a gradient of Percoll. The technique with Percoll has advantages as compared with the method of adherence: 1. Greater purity of monocyte suspension and higher percentage of monocytes (85% of monocytes on an average compared with 60% in adherence). 2. A damage of membrane can sometimes be observed in those monocytes gained by adherence and as a consequence of that there is a tendency to unspecifically positive cytotoxic reactions. In monocytes isolated by means of these two methods HLA-DR antigens could be identified. There was no complete correspondence between the cytotoxical reactions with monocytes and B-lymphocytes.
D001402:B-Lymphocytes / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II
Versuch der Verwendung von Monozyten zur HLA-DR-Typisierung.