Methylation of a middle repetitive DNA sequence class during differentiation in Friend erythroleukemia cells.
We have examined the methylation patterns within middle repetitive sequences in Friend erythroleukemia cells. Mouse-interspersed-family-1 (MIF-1) and a group characterized by a 1350-bp Eco-Bam fragment cloned into pBR322 as plasmid pFS-13, are both less modified in Friend cell DNA than in normal tissue DNA. The pattern of methylation present in pFS-13 homologous sequences was found to be stable during cell division, i.e., somatically inherited, and stable during differentiation induced by HMBA.
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D019003:Deoxyribonuclease HpaII; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D008745:Methylation; D051379:Mice; D008811:Mice, Inbred DBA; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000032:analysis
D000081:Acetamides; D004274:DNA, Recombinant; D004247:DNA; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; C014026:hexamethylene bisacetamide