T-cell hybridoma specific for a cytochrome c peptide: specific antigen binding and interleukin 2 production.
T-cell hybridomas were obtained after fusion of BW 5147 thymoma and long-term cultured T cells specific for cytochrome c peptide 66-80 derivatized with a 2,4-dinitroaminophenyl (DNAP) group. The resulting hybridomas were selected for their capacity to specifically bind to soluble radiolabeled peptide antigen. One T-cell hybrid was positive for antigen binding. This hybrid T cell exhibits surface phenotypic markers of the parent antigen-specific T cells. The binding could be inhibited either by an excess of unlabeled homologous antigen or by cytochrome c peptide 11-25 derivatized with a 2-nitrophenylsulfenyl group. Several other peptide antigens tested failed to inhibit binding of the radioactive peptide. This suggests that a specific amino acid sequence, modified by a DNAP group, is the antigenic structure recognized by the putative T-cell receptor. In addition, direct interaction of DNAP-66-80 peptide with the hybridoma cell line induced production of the T-cell growth factor interleukin 2. Furthermore, supernatants derived from syngeneic macrophages pulsed with the relevant peptide also induced the antigen-specific hybridoma to produce interleukin 2.
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003574:Cytochrome c Group / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013945:Thymoma / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology
D003574:Cytochrome c Group; D000939:Epitopes; D007376:Interleukin-2; D010446:Peptide Fragments