Prostaglandin-generating factor of anaphylaxis. II. Characterization of activity.
Anaphylaxis of lung tissue leads to the generation of prostaglandins, thromboxanes, and hydroxyeicosatetraenoic acids. A portion of the prostaglandin generation is due to a unique oligopeptide termed "prostaglandin-generating factor of anaphylaxis (PGF-A)," which is generated during anaphylaxis. The activity of PGF-A on human and guinea pig lung was characterized: peak PG formation occurs within 5 to 10 min of the addition of PGF-A, calcium but not magnesium is required for PGF-A activity, and both physiologic pH and temperature are necessary for optimal activity. Human and guinea pig airway and parenchymal (lung) responses to PGF-A were similar (predominantely PGF2 alpha) but were not identical to each other (guinea pig parenchyma produces thromboxane as well). PGF-A is, thus, a novel, potent mediator generated during anaphylaxis of lung tissue that may contribute to the spectrum of tissue responses seen in allergic reactions.
D000707:Anaphylaxis / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic; D002413:Cations, Divalent; D006636:Histamine Release; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E; D008168:Lung / Q000276:immunology*; D009842:Oligopeptides / Q000302:isolation & purification*; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D013696:Temperature; D013931:Thromboxanes / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D002413:Cations, Divalent; D009842:Oligopeptides; D011458:Prostaglandins E; D011460:Prostaglandins F; D013931:Thromboxanes; C035516:prostaglandin-generating factor of anaphylaxis; D007073:Immunoglobulin E