Cytotoxic T lymphocyte recognition of the influenza hemagglutinin gene product expressed by DNA-mediated gene transfer.
We have used the technique of DNA-mediated gene transfer to examine cytotoxic T lymphocyte (CTL) recognition of the product of the cloned A/JAPAN/305/57 hemagglutinin (HA) gene in murine (L929) cells. Using both heterogeneous and homogeneous (clonal) populations of type A influenza-specific CTL, we have demonstrated that the HA molecule can serve as a target antigen for both the subtype-specific and the cross-reactive subpopulations of influenza-specific CTL. Our results also raise the possibility that other virus-specified polypeptides may serve as target molecules for cross-reactive CTL.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology*; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D000939:Epitopes; D006389:Hemagglutinins, Viral