Molecular cloning and sequence analysis of trp-lac fusion deletions.
DNA fragments containing deletions that fuse the trp operon to the lac operon were cloned and the end-points of the fusions were determined. The results from DNA sequence analysis correlated well with those from genetic, biochemical and physiological studies previously reported. The sequence data from this study, in combination with the known properties of these fusion strains, provided information on: (1) the precise lac operon distal boundary of the lac operator; (2) the nature of the trp operon terminator; and (3) the messenger RNA sequences that result in inhibition of lacZ translation initiation in trp-lac fused mRNAs.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D009690:Nucleic Acid Conformation; D009876:Operon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014364:Tryptophan / Q000235:genetics*; D029968:Escherichia coli Proteins
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; D014364:Tryptophan; C027190:TRPR protein, E coli; D029968:Escherichia coli Proteins