title: Defined salmonella antigens for detection of cellular and humoral immune responses in salmonella infected calves.
abstract: Intracutaneous injection of a crude supernatant fraction from homogenised Salmonella typhimurium SVA 44 (O 4, 5, 12) or S dublin SVA 47 (O 9, 12) elicited highly significant (P less than 0.005) double skin-fold thickness increases in calves spontaneously infected with salmonella and verified as excretors. The use of isolated structurally defined outer membrane components from salmonella bacteria established that the delayed skin reactions could be elicited by either the lipopolysaccharide which contains O-antigenic polysaccharide chains homologous to the infecting strain, or an outer membrane protein fraction (porin). The porin preparation gave rise to skin reactions regardless of which salmonella serotype the calf was infected with. Histological examination of biopsy material indicated a delayed skin reaction. No such reactions were seen in biopsies from control calves. The use of lipopolysaccharide permitted a salmonella serogroup specific skin test although the endotoxic side effects were marked in doses above 50 micrograms. Purified O-antigen specific polysaccharides devoid of lipid A from S typhimurium (O 4, 12) or S enteritidis (O 9, 12) failed however to elicit skin reactions. Infected calves had humoral antibody titres against the O antigen of the infecting strain which were significantly (P less than 0.005) higher than those found in control calves.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006968:Hypersensitivity, Delayed / Q000209:etiology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D012475:Salmonella / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology; D012882:Skin Tests / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides