Double staining of the muscle fibers and interstitial tissue components for automatic analysis of muscle biopsies.
A staining technique is presented in order to facilitate the automatic analysis of muscle biopsies. It renders possible the visualization on the same section of the myofibrillary adenosine-triphosphatase (ATPase) activity under the microscope with transmitted light, and of the fluorescent staining of fibronectin which marks the outlines of the muscle fibers by a simple switch to ultraviolet (UV) light.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001706:Biopsy; D005353:Fibronectins / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D009132:Muscles / Q000201:enzymology* / Q000473:pathology; D011817:Rabbits; D013194:Staining and Labeling / Q000379:methods
D005353:Fibronectins; D000251:Adenosine Triphosphatases