Strain-specific determinants on the major phosphoprotein of murine mammary tumor viruses.
The major phosphoprotein (p23) of murine mammary tumor virus (MuMTV) was purified for C3H virus, using a combination of alkyl agarose chromatography and gel filtration. Monospecific antiserum was raised against glutaraldehyde crosslinked polymers of purified p23, and the antiserum was then used with iodinated p23 to develop a competition radioimmunoassay. Phosphoprotein p23 was shown to contain both group- and type-specific antigenic determinants in assays using detergent-disrupted virions of MuMTV strains C3H, RIII, GR, and DBA/2 as competing antigens. Tryptic and chymotryptic peptide mapping analysis of p23 polypeptides isolated from MuMTV strains C3H, RIII, GR and DBA/2 showed that the p23s of each virus strain could be clearly distinguished from each other.
D000956:Antigens, Viral / Q000276:immunology*; D000939:Epitopes; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis / Q000145:classification / Q000276:immunology*; D010750:Phosphoproteins / Q000276:immunology* / Q000302:isolation & purification; D011863:Radioimmunoassay; D013045:Species Specificity; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification
D000956:Antigens, Viral; D000939:Epitopes; D010750:Phosphoproteins; D014758:Viral Core Proteins; D014764:Viral Proteins