Effect of gamma interferon preparations on in vitro phagocytosis and degradation of Escherichia coli by mouse peritoneal macrophages.
Treatment of mouse peritoneal macrophages by gamma (type II, immune) interferon depressed the ingestion of non-opsonized Escherichia coli mediated by the non specific receptor, and also the intracellular degradation of the ingested bacteria. These effects were time and dose-dependent, and sensitive for trypsin and pH 2 treatment. The intracellular concentration of three lysozomal enzymes, beta-glucuronidase, acid phosphatase and cathepsin D, was elevated in gamma interferon-treated macrophages.
D000135:Acid Phosphatase / Q000378:metabolism; D001202:Ascitic Fluid / Q000166:cytology; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D002403:Cathepsins / Q000378:metabolism; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology; D005966:Glucuronidase / Q000378:metabolism; D007372:Interferons / Q000494:pharmacology*; D008214:Lymphocytes; D008264:Macrophages / Q000201:enzymology / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*
D007372:Interferons; D000135:Acid Phosphatase; D005966:Glucuronidase; D002403:Cathepsins