title: Specificity of IgM antibodies in acute human coxsackievirus B infections, analysed by indirect solid phase enzyme immunoassay and immunoblot technique.
abstract: The specificity of the IgM response in acute human coxsackievirus B infections was examined by indirect solid phase enzyme immunoassay and immunoblot techniques. IgM antibodies detected by ELISA were either strictly type-specific, type-predominant or group-reactive to coxsackieviruses B-1 to B-5. Homotypic and type-dominant responses were clearly correlated with the serotype isolated from the patient. Analysis of ELISA antigens by SDS-gel electrophoresis, protein transfer and subsequent immunodetection of individual virus polypeptides, revealed VP1 as the major antigen; it was detected by homotypic as well as heterotypic serum specimens.
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D003384:Coxsackievirus Infections / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology*; D007158:Immunologic Techniques; D014764:Viral Proteins / Q000276:immunology; D015678:Viral Structural Proteins
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D007075:Immunoglobulin M; D014764:Viral Proteins; D015678:Viral Structural Proteins