Multiple specificities of brain Ca2+- and calmodulin-dependent protein kinase for substrate.
The purified Ca2+- and calmodulin-dependent protein kinase from rat brain, which has a M.W. of 120,000 by gel filtration analysis, showed a broad substrate specificity. In addition to myosin light chain from chicken gizzard, the enzyme phosphorylated myelin basic protein, casein and two endogenous substrates in a Ca2+- and calmodulin-dependent manner. In contrast, chicken gizzard myosin light chain kinase exclusively phosphorylated myosin light chain.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000201:enzymology*; D002364:Caseins / Q000378:metabolism; D002645:Chickens; D004589:Electrophoresis, Disc; D005895:Gizzard, Avian / Q000201:enzymology; D004676:Myelin Basic Protein / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase; D009218:Myosins / Q000378:metabolism; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity
D002364:Caseins; D004676:Myelin Basic Protein; D010770:Phosphotransferases; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase; D009218:Myosins