Isolation and characterization of snRNA and scRNA gene candidates from a human genomic library.
A simple procedure for the isolation and preparative gel electrophoresis of snRNA and scRNA is described. These small RNA species were used to select DNA sequences from a human genomic library which are able to protect hybridized snRNA or scRNA against T1-ribonuclease attack. The snRNA clones obtained contain only sequences for one snRNA species and only one copy of the respective gene. In contrast, more than one 7S RNA gene is present within the scRNA clones.
D003001:Cloning, Molecular; D003593:Cytoplasm; D005796:Genes; D008040:Genetic Linkage; D006367:HeLa Cells; D006801:Humans; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012342:RNA, Small Nuclear
D012342:RNA, Small Nuclear; D012313:RNA