Complexity of the histocompatibility-3 region in the mouse.
Congenic lines C57BL/10Sn (H-3a), B10.LP-H-3b, B10.C-H-3c, and B10.KR-H-3d were arranged into the 12 possible recipient-donor combinations and immunized. The spleen cells of each recipient were then tested for the presence of cytolytic effector T lymphocytes (CTL) specific for antigenic determinants controlled by the H-3 locus and restricted by class I H-2 loci. The strain-distribution pattern of the determinants defined by the individual CTL was determined by direct testing and by cold-target inhibition analysis. The restriction elements of the individual determinants were identified by the testing of H-2 recombinant strains and by antibody blocking. Five determinants were identified: 1 and 2, restricted by the Db molecule; 3 and 4 restricted by the Kb molecule; and 5, for which the restriction molecule could not be determined. The distribution pattern of determinants 3 and 4 correlated with the distribution of alleles at the beta 2-microglobulin locus. The data could be interpreted as arguing for the existence of three loci in the H-3 complex, with one coding for mutually antithetical determinants 1 and 2, another coding for mutually antithetical determinants 3 and 4, and still another coding for determinant 5.
D000818:Animals; D001667:Binding, Competitive; D002874:Chromosome Mapping; D003429:Cross Reactions; D003433:Crosses, Genetic; D000939:Epitopes / Q000032:analysis / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics; D007519:Isoantigens / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology*; D008912:Minor Histocompatibility Loci; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000939:Epitopes; D006183:H-2 Antigens; D007519:Isoantigens