title: Identification of a sialic acid containing glycocalyx on the surface of Schistosoma mansoni.
abstract: Ruthenium red fixation of adult Schistosoma mansoni revealed the existence of a negatively charged layer external to the outer bilayer, which was morphologically similar to the glycocalyx of other cell types. Regional and sexual differences were found in the extent and organisation of the surface coat, which can be correlated with interfacial free energy, adhesiveness and protection from immune effectors. Neuraminidase treatment confirmed the presence of surface sialic acid. Mechanical or skin penetrated schistosomula, maintained in vitro for 24 h were found not to have a glycocalyx and this may relate to their increased susceptibility to immune killing. Lung stage schistosomula however, did bind ruthenium red to their surface.
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D012430:Ruthenium Red; D012550:Schistosoma mansoni / Q000032:analysis* / Q000648:ultrastructure; D012794:Sialic Acids / Q000032:analysis*
chemical_list: D012794:Sialic Acids; D012430:Ruthenium Red