Abelson murine leukemia virus P120: identification and characterization of tyrosine phosphorylation sites.
Tryptic peptides containing two major in vivo P120gag-abl tyrosine phosphorylation acceptor sites were identified, phosphorylated in vitro, and purified to homogeneity. The tyrosine site in peptide a is localized at a position six residues distal to its trypsin cleavage site, whereas the tyrosine acceptor site in peptide b is at residue seven. A third peptide, c, contains an amino-terminal phosphotyrosine residue: phosphorylation of this latter peptide only occurs to a significant extent in vivo.
D000011:Abelson murine leukemia virus / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D010766:Phosphorylation; D019000:Phosphotyrosine; D014357:Trypsin; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis; D014764:Viral Proteins / Q000378:metabolism*
D010446:Peptide Fragments; D014764:Viral Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D014357:Trypsin