title: Isolation and biological characterization of mouse LM fibroblast variants resistant to the cytotoxicity of polyriboinosinic polyribocytidylic acid after interferon treatment.
abstract: Mouse LM fibroblasts growing continuously in the absence of serum have an increased sensitivity to the cytotoxicity of poly(rI) X poly(rC) after interferon (IFN) exposure. This has allowed the isolation by an enrichment procedure of several independent and stable variant clones (IFN + I X C)R which are resistant to such a treatment. One of the resistant variants has been more extensively characterized as far as IFN action and IFN production are concerned. It behaves identically to the wild-type parent except for the spontaneous release of low amounts of IFN. The target of the mutation probably resides in a late step in the development of the cytotoxic response as revealed by microinjection techniques. The (IFN + I X C)R variants characterized here thus appear different from mutants in the IFN system isolated so far.
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects; D007372:Interferons / Q000096:biosynthesis / Q000494:pharmacology*; D007621:Karyotyping; D051379:Mice; D010641:Phenotype; D011070:Poly I-C / Q000494:pharmacology*
chemical_list: D007372:Interferons; D011070:Poly I-C