Stimulation of adenosine 3',5'-monophosphate formation in mononuclear leukocytes by toad venoms.
The content of adenosine 3',5'-monophosphate in human mononuclear leukocytes was enhanced 3-5-times by venoms obtained from African toad (Bufo africanus), American toad (Bufo americanus), Colorado river toad (Bufo arenarum) and Marine toad (Bufo marinus) at 25 micrograms/ml for 5 min of incubation at 37 degrees C. The maximum stimulation was observed after 1-5 min of incubation. The half-maximal stimulation was observed at 0.1 micrograms/ml venom obtained from Colorado river toad (Bufo arenarum). The increased content of adenosine 3',5'-monophosphate in the mononuclear leukocytes persisted without significant change for at least 30 min of incubation at 37 degrees C.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000527:Alprostadil; D000664:Amphibian Venoms / Q000494:pharmacology*; D000818:Animals; D002022:Bufo arenarum; D002024:Bufo marinus; D002025:Bufonidae; D000242:Cyclic AMP / Q000097:blood*; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D013045:Species Specificity
D000664:Amphibian Venoms; D011458:Prostaglandins E; D000242:Cyclic AMP; D000527:Alprostadil; D015056:1-Methyl-3-isobutylxanthine