title: [Staining behavior and applicability of spectrally pure Capriblue GN, Stella Blue, Oxonin and Punky Blue].
abstract: The staining properties of 4 spectral pure derivates of Phenoxazin--Capriblue GN, Stella Blue, Oxonin, and Punky Blue--were investigated using human and animal tissues. Punky Blue and Stella Blue are newly synthesized derivates. A simplified staining technique was developed based on conventional fixing and mounting methods of histological materials. Punky Blue is a particularly contrasting metachromatic nucleus stain, and produces at pH = 4 and a staining period of 5 min a differentiated cell picture, the colours of which are comparable to conventional stains. The Oxonin-stain produces good contrasts after 2 min. Capriblue GN is only limited use as one-component tissue stain, due of its low metachromatic characteristics and Stella Blue, on its own, does not provide sufficient contract. The influence of electrolytes and hydrogen ions on the staining mechanisms will be discussed. An increase of hydrogen ions is used to control the selective staining of amphoteric biopolymers. The possible binding mechanisms of stains used are discussed in the light of their staining properties.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D004573:Electrolytes; D006651:Histocytochemistry; D010078:Oxazines; D013194:Staining and Labeling
chemical_list: D004573:Electrolytes; D010078:Oxazines; C038617:capriblue GN; C038620:oxonin; C038619:punky blue; C038618:stella blue
vernacular_title: Uber das Färbeverhalten und die Anwendbarkeit von spektralreinem Capriblau GN, Stella Blue, Oxonin und Punky Blue.