Automated, quantitative cytopathic effect reduction assay for interferon.
A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.
D001681:Biological Assay; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000502:physiology; D006801:Humans; D007372:Interferons / Q000032:analysis*; D013053:Spectrophotometry
D007372:Interferons