Influenza A hemagglutinin-specific T cell clones strictly restricted by HLA-DR1 or HLA-DR7 molecules.
The antigenic specificities, major histocompatibility complex restrictions and functional properties of influenza virus-specific proliferative cloned cell lines have been studied. These lines were specific for the H3 hemagglutinin subtype of influenza A viruses. By using a large panel of HLA-phenotyped antigen-presenting cells, it was found that the polymorphic structures, defined as DR1 and DR7 molecules, or closely associated structures, function as the restricting elements. We excluded for these lines a possible restricting role of supertypic specificities, known cross-reacting elements on DR molecules, or products of other loci in known linkage disequilibrium with the HLA-DR molecules. Such exquisitely restricted clones might be of great help in the class II typing of antigen-presenting cells. Their specific activity was stable for several months. This has allowed the study of some functional properties of these long-term-cultured cloned cell lines: interleukin 2 sensitivity and production, helper function in specific antibody synthesis and ability to stimulate in mixed leukocyte reactions.
D000328:Adult; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005802:Genes, MHC Class II; D015798:HLA-DR1 Antigen; D015809:HLA-DR7 Antigen; D006389:Hemagglutinins, Viral / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology; D008211:Lymphocyte Cooperation; D007959:Lymphocyte Culture Test, Mixed; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D015798:HLA-DR1 Antigen; D015809:HLA-DR7 Antigen; D006389:Hemagglutinins, Viral; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2