A site in a tRNA precursor that can be processed by the whole RNase P enzyme but not by the RNA alone.
A precursor molecule for 10 Sb RNA, the RNA moiety of the RNA processing enzyme RNase P, was purified, characterized for enzymatic activity, and compared to 10 Sb RNA and to RNase P. In these studies the K RNA, a dimeric precursor of tRNAGln-tRNALeu, coded by bacteriophage T4, was used as a substrate. This precursor contains two RNase P cleavage sites, one at each 5' end of the two tRNAs. The precursor 10 Sb and 10 Sb RNAs have the capacity to cleave the precursor tRNA molecule but only at the 5' end of tRNALeu, not at the 5' end of tRNAGln. Even when a substrate was prepared that contained only one site for RNase P (the one next to tRNAGln), this substrate was not cleaved by the RNA alone while the whole enzyme was effective in processing this substrate. The possible function of the protein of RNase P in the enzymatic reaction is discussed.
D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009694:Nucleic Acid Precursors / Q000235:genetics*; D010957:Plasmids; D012313:RNA / Q000378:metabolism*; D012322:RNA Precursors; D012323:RNA Processing, Post-Transcriptional; D012343:RNA, Transfer / Q000235:genetics*; D043262:Ribonuclease P
D029968:Escherichia coli Proteins; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012313:RNA; D012343:RNA, Transfer; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli