[Analysis of lymphocyte adhesion and the phenomenon of immunologic adhesion inhibition].
Investigations were performed in rabbits to elucidate the cell physiological parameters of the inhibitor reaction of adhesion. For this purpose, an improved method of measuring the cell adhesion was used. The well-known fact that culture cells will reveal a much stronger reaction could be confirmed. This may be due to the high increase of T-lymphocytes because of findings concerning cell differentiation with acid alpha-naphthylacetate esterase (ANAE), whereas no corresponding results could be found for the particular role of macrophages. It could be proved that the inhibitor reaction already began 20 minutes after the antigen contact. On the basis of these facts beta-lymphotoxin might be assumed to play a part in the inhibitor reaction. A direct relationship between oxygen consumption and the inhibitor reaction of adhesion could not be proved. The respiratory depression frequently observed might be due to the cytotoxic effect of excreted lymphotoxins. As the inhibitor reaction of adhesion could be identified even after 1-1 1/2 years, it can be regarded as a longterm immunophenomenon. The findings with metabolic blockers underline that the primary adhesion of lymphocytes is supported by the oxidative metabolism and not by glycolysis which is generally characteristic of granulocytes.
D000818:Animals; D000941:Antigens / Q000276:immunology; D002448:Cell Adhesion; D006019:Glycolysis; D007158:Immunologic Techniques; D007957:Leukocyte Adherence Inhibition Test; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D010101:Oxygen Consumption; D011817:Rabbits
D000941:Antigens; D008233:Lymphotoxin-alpha
Untersuchungen zur Haftung von Lymphozyten und zum Phänomen der immunologischen Haftungshemmung.