The characterization of cDNA clones coding for wheat storage proteins.
Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley storage protein, B-hordein. The sequence includes a tandem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D014908:Triticum / Q000235:genetics
D010940:Plant Proteins; D012333:RNA, Messenger; C038234:storage protein, Triticum aestivum; D011061:Poly A; D012313:RNA; D004247:DNA