PHA-induced neutrophil-mediated cytotoxicity.
Neutrophils activated by phytohemagglutinin (PHA) were cytotoxic to chicken red blood cells (CRBC), as determined by the 51Cr release assay. High levels of cytotoxicity were obtained when one CRBC was available for each effector cell. Neutrophils from patients with Chronic Granulomatous Disease (CGD), incapable of generating potentially toxic oxygen radicals, had a reduced cytotoxicity activity, suggesting the requirement for an intact oxidative metabolism in the PHA-induced neutrophil-mediated cytotoxicity. However, CGD neutrophils maintained approximately 50% of this activity, compared with normal cells. These findings support the conclusion that both oxygen-dependent and oxygen-independent mechanisms are necessary to achieve an efficient cytotoxicity.
D000293:Adolescent; D007611:Aprotinin / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000276:immunology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D010101:Oxygen Consumption; D010835:Phytohemagglutinins / Q000494:pharmacology*
D010835:Phytohemagglutinins; D006493:Heparin; D007611:Aprotinin