Selective extraction by 1-butanol of surface glycoprotein antigens from human melanoma cells.
The butanol extraction method has previously been used to achieve selective release of tumor-specific transplantation antigens from mouse sarcoma cells. In this study we investigated the feasibility of this method for extracting four surface glycoprotein antigens (87K, 95-150K, HLA-DR, and HLA-A,B,C) from cultured human melanoma cells. Of the four antigens examined, only 95-150K and HLA-DR antigens could readily be detected in material extracted by 2%, 3%, or 5% butanol. The 3% butanol was found to be most effective in releasing these two antigens. Treatment of melanoma cells with less than or equal to 3% butanol did not decrease the viability of extracted cells as judged by either Trypan Blue dye exclusion or plating efficiency. Thus the noncytolytic butanol extraction method offers a promising approach to the isolation of certain glycoproteins such as 95-150K and HLA-DR from viable human melanoma cells for further purification and structural analysis.
D020001:1-Butanol; D000951:Antigens, Neoplasm; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D000440:Butanols / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D000939:Epitopes / Q000032:analysis; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008545:Melanoma / Q000276:immunology* / Q000378:metabolism; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000440:Butanols; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D020001:1-Butanol