Location and distribution of the light chains in clathrin trimers.
Mouse monoclonal antibodies have been made that are specific for one of the light chains (LC-A) of calf brain clathrin. The determinant they recognize has been mapped by immunoelectron microscopy and shown to lie near the center of a clathrin trimer. Quantitative immunoprecipitation experiments with one of the monoclonal antibodies suggests that, despite the overall 1:2 stoichiometry, the light chain composition of an individual clathrin trimer is nearly random.
D000818:Animals; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D002966:Clathrin; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D002966:Clathrin; D000939:Epitopes; D046911:Macromolecular Substances; D008565:Membrane Proteins