Isolation and characterization of Drosophila melanogaster U2 small nuclear RNA genes.
We describe here the organization of DNA sequences complementary to Drosophila melanogaster U2 small nuclear (sn) RNA. From a genomic library we isolated two recombinants containing two genes each. Genomic reconstruction experiments and Southern analysis revealed that D. melanogaster possesses only four to five U2 snRNA genes or very closely related sequences. The nucleotide sequence of one of the clones analysed shows 77% homology with rat U2 snRNA. A stretch of 12 nucleotides that has been implicated in heterogeneous nuclear RNA splicing is conserved between rat and Drosophila. The genomic organization of these genes is very similar in different melanogaster strains but diverges highly in different Drosophila species.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D005838:Genotype; D008854:Microscopy, Electron; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D012342:RNA, Small Nuclear
D009692:Nucleic Acid Heteroduplexes; D012342:RNA, Small Nuclear; D012313:RNA; D004247:DNA