Origin of peptide-containing fibers in the inferior mesenteric ganglion of the guinea-pig: immunohistochemical studies with antisera to substance P, enkephalin, vasoactive intestinal polypeptide, cholecystokinin and bombesin.
After different denervation procedures the guinea-pig inferior mesenteric ganglion was analysed by immunohistochemistry using antisera to substance P, enkephalin, vasoactive intestinal polypeptide, cholecystokinin and bombesin. The results demonstrate that each of the nerve trunks connected  to the ganglion carries specific peptidergic pathways. Thus, the lumbar nerves contain substance P-immunoreactive primary afferent neurons, which to a large extent traverse the ganglion, and enkephalin-immunoreactive preganglionic neurons; the colonic nerves carry vasoactive intestinal polypeptide-, cholecystokinin- and bombesin-immunoreactive fibers from the distal colon to the ganglion; the hypogastric nerves contain vasoactive intestinal polypeptide-positive fibers from the pelvic plexus; and the intermesenteric nerve contains vasoactive intestinal polypeptide, cholecystokinin, substance P and enkephalin from divergent sources. By studying accumulations of peptides in ligated lumbar splanchnic, intermesenteric, hypogastric and colonic nerves the existence of these major peptidergic pathways was confirmed and evidence was obtained for additional, not so prominent, peptidergic projections. The results arae discussed in view of earlier morphological and physiological studies.
D000818:Animals; D001839:Bombesin / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism; D003106:Colon / Q000294:innervation; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005728:Ganglia, Sympathetic / Q000033:anatomy & histology*; D006168:Guinea Pigs; D007001:Hypogastric Plexus / Q000033:anatomy & histology; D008297:Male; D008643:Mesentery / Q000294:innervation; D009412:Nerve Fibers / Q000648:ultrastructure*; D010455:Peptides / Q000378:metabolism*; D013153:Splanchnic Nerves / Q000033:anatomy & histology; D013373:Substance P / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D010455:Peptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D004744:Enkephalin, Methionine; D002766:Cholecystokinin; D004299:Dopamine beta-Hydroxylase; D001839:Bombesin