Cytochemical demonstration of the molecular forms of cardiac glycosides in the heart muscle.
Selective topo-optical staining of vicinal-OH groups with aldehyde-bisulphite-toluidine blue (ABT) has been used for studying the localization and molecular structural order of cardiac glycoside in heart muscle. The glycoside has strong metachromatic basophilia and negative birefringence. In addition to confirming the electron microscopic suitability of the ABT reaction has offered also ultrastructural evidence for the reliability of the topo-optical method. The localization appeared to be in extracellular connection with the outer side of sarcolemma membrane, internal surface of endothelial cell, and in the subsarcolemmal cysterne. The findings suggest that the topo-optical reaction is suitable for molecular analysis of cardiac glycoside.
D000447:Aldehydes; D000818:Animals; D002301:Cardiac Glycosides / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004285:Dogs; D006651:Histocytochemistry; D009206:Myocardium / Q000032:analysis* / Q000648:ultrastructure; D013194:Staining and Labeling; D013447:Sulfites; D014048:Tolonium Chloride
D000447:Aldehydes; D002301:Cardiac Glycosides; D013447:Sulfites; D014048:Tolonium Chloride