title: Nucleic acid and histone synthesis by ethanol-treated cleavage-arrested sea urchin embryos.
abstract: It has been found that fertilized sea urchin eggs prevented from normal cleavage by solutions of isosmotic ethanol in sea water are able to complete some cellular and molecular aspects of the normal developmental program that are observed in control cultures. In both treated and control cultures, the type of RNA transcribed changes at 24 h (early gastrula) in favor of higher molecular weight rRNA. Ultrastructural studies reveal the presence of nucleoli in ethanol-treated as well as control embryos. The type of H1 histone synthesized also shifts at 24 h in favor of a higher molecular weight H1 in both ethanol-treated and control embryos. Replication of DNA proceeds at a slower rate in ethanol-treated embryos than in controls, resulting in DNA/embryo values in ethanol which are 20-30% of control values after 24 h. The results relate to the problem of differentiation without cleavage, and the role of normal partitioning, cell-cell interaction, and DNA synthesis in triggering the sequence of events in the developmental program.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006657:Histones / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D012617:Sea Urchins; D015053:Zygote / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006657:Histones; D000431:Ethanol; D012313:RNA; D004247:DNA