Demonstration of the polysaccharides in the cell wall of Candida albicans blastospores, using silver methenamine staining and a sequence of extraction procedures.
Blastospores of Candida albicans were subjected to a series of extractions of increasing severity to remove polysaccharides from the wall. The procedure was observed by electron microscopy, using silver methenamine staining to localize the polysaccharides.
D002176:Candida albicans / Q000032:analysis / Q000502:physiology* / Q000648:ultrastructure; D002473:Cell Wall / Q000032:analysis; D008709:Methenamine; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D013048:Specimen Handling; D013172:Spores, Fungal / Q000032:analysis / Q000648:ultrastructure; D013194:Staining and Labeling
D011134:Polysaccharides; D008709:Methenamine