Studies of the growth of human bone-derived cells in culture using aqueous two-phase partition.
Human bone cells, maintained in culture, have been subjected to partitioning in an aqueous two-phase system on a countercurrent distribution apparatus. A broad cell distribution was obtained indicating cell-surface heterogeneity. Two major cell populations were identified which appeared to be growing at different rates. The 'fast'-growing cells had a less hydrophobic cell surface than the 'slow'-growing cells. Possible relationships of these cell populations with osteoblast differentiation and the potential importance of this technique in studies of osteoblast differentiation are discussed.
D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003377:Countercurrent Distribution; D003911:Dextrans; D006801:Humans; D010006:Osteoblasts / Q000502:physiology*; D011092:Polyethylene Glycols
D003911:Dextrans; D011092:Polyethylene Glycols