Preparation and antigenic property of 3-dehydrolithocholylglycine 3-(O-carboxymethyl) oxime-bovine serum albumin conjugate.
The preparation and antigenic property of 3-dehydrolithocholyglycine-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-carboxymethyl) oxime bridge at the C-3 position on the steroid nucleus is described. Antibody raised against antigen in the rabbit possessed high titer and specificity to lithocholylglycine, exhibiting no significant cross-reaction with free lithocholic acid or lithocholyltaurine.
D000936:Antigen-Antibody Complex; D000939:Epitopes / Q000032:analysis; D006241:Haptens; D007106:Immune Sera; D007202:Indicators and Reagents; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D009682:Magnetic Resonance Spectroscopy; D012710:Serum Albumin, Bovine; D013055:Spectrophotometry, Infrared
D000936:Antigen-Antibody Complex; D000939:Epitopes; D006241:Haptens; D007106:Immune Sera; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D008095:Lithocholic Acid; C040171:3-dehydrolithocholylglycine