Cloning and characterization of cDNAs encoding human gastrin-releasing peptide.
We have prepared and cloned cDNAs derived from poly(A)+ RNA from a human pulmonary carcinoid tumor rich in immunoreactivity to gastrin-releasing peptide, a peptide closely related in structure to amphibian bombesin. Mixtures of synthetic oligodeoxyribonucleotides corresponding to amphibian bombesin were used as hybridization probes to screen a cDNA library prepared from the tumor RNA. Sequencing of the recombinant plasmids shows that human gastrin-releasing peptide (hGRP) mRNA encodes a precursor of 148 amino acids containing a typical signal sequence, hGRP consisting of 27 or 28 amino acids, and a carboxyl-terminal extension peptide. hGRP is flanked at its carboxyl terminus by two basic amino acids, following a glycine used for amidation of the carboxyl-terminal methionine. RNA blot analyses of tumor RNA show a major mRNA of 900 bases and a minor mRNA of 850 bases. Blot hybridization analyses using human genomic DNA are consistent with a single hGRP-encoding gene. The presence of two mRNAs encoding the hGRP precursor protein in the face of a single hGRP gene raises the possibility of alternative processing of the single RNA transcript.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001839:Bombesin / Q000235:genetics*; D002276:Carcinoid Tumor / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D019886:Gastrin-Releasing Peptide; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics*; D011061:Poly A / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics
D010455:Peptides; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D019886:Gastrin-Releasing Peptide; D004247:DNA; D004262:DNA Restriction Enzymes; D001839:Bombesin