title: Secretion and glycosylation of alpha-foetoprotein by the mouse yolk sac.
abstract: Secretion and glycosylation of alpha-foetoprotein (AFP) by mouse yolk sac were studied by using yolk-sac explants cultured in vitro. Yolk-sac explants rapidly incorporated [35S]methionine into AFP, whereas radioactively labelled AFP was not found in the medium until 30 min after incubation was initiated. Electrophoretic analysis revealed that microheterogeneity of AFP synthesized in explants increased in parallel with the gestational age of the yolk sacs. The change in microheterogeneity was noted by the formation of increasingly acidic forms. Only the most acidic forms of AFP were found to be present in the medium on each gestational day studied. Tunicamycin reduced the incorporation of glucosamine into AFP with a concomitant decrease in molecular weight and microheterogeneity. However, the relative amount of AFP released into the medium was not altered by the presence of tunicamycin. The presence of under-glycosylated AFP in the medium indicates that glycosylation of AFP is not essential for its secretion from the yolk sac. In light of these and previous findings, it is suggested that the glycosylation of AFP may be important for the turnover of this glycoprotein in serum.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004527:Egg Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D014415:Tunicamycin / Q000494:pharmacology; D015017:Yolk Sac / Q000187:drug effects / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D000509:alpha-Fetoproteins; D014415:Tunicamycin; D005944:Glucosamine