Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110.
The nucleotide sequence of the promotor and NH2-terminal signal peptide region of the alpha-amylase gene derived from the alpha-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH2-terminal region of the mature alpha-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the alpha-amylase gene of B. amyloliquefaciens.
D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D009876:Operon; D010455:Peptides / Q000302:isolation & purification*; D021382:Protein Sorting Signals; D000516:alpha-Amylases / Q000235:genetics*
D004269:DNA, Bacterial; D010455:Peptides; D021382:Protein Sorting Signals; D000681:Amylases; D000516:alpha-Amylases