Pulse and steady-state labelled and actinomycin D chased 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)-resistant hnRNA from uninfected HeLa cells.
The fraction of hnRNA synthesized in the presence of DRB in uninfected HeLa cells ranges in size from 4S to over 45S. High molecular weight DRB-resistant hnRNA has been demonstrated to decay after 2 h of actinomycin D chase. This fraction is composed of both poly(A)(+) and poly(A)(-) RNA molecules. The synthesis in the presence of DRB of short polyadenylated hnRNA was also observed. The nature of both hnRNA subfractions is discussed.
D003609:Dactinomycin / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology*; D004351:Drug Resistance; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis*; D012333:RNA, Messenger; D012263:Ribonucleosides / Q000494:pharmacology*
D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D012263:Ribonucleosides; D003609:Dactinomycin; D011061:Poly A; D004004:Dichlororibofuranosylbenzimidazole; D012313:RNA