title: Inhibition of phytohaemagglutinin-induced autorosette formation of human peripheral blood lymphocytes by RNA or protein synthesis inhibitor.
abstract: The induction of autorosette forming cells (ARFC) with phytohaemagglutinin (PHA) in human peripheral blood lymphocytes (PBL) was examined. After 24 h of incubation with PHA, the level of ARFC in PBL was markedly increased but subsequently decreased to about one-third of the peak level at 96 h of culture. When PBL were pre-treated with actinomycin D, or cultured with puromycin, the induction of ARFC was completely blocked. Pre-treatment of PBL with mitomycin C (MMC) had no effect on induction of ARFC, whereas DNA synthesis was completely blocked. These data indicate that the generation of autologous red blood cell receptors is a relatively early event in PHA activated PBL, and that it is independent of DNA synthesis but dependent on RNA and protein synthesis.
mesh_terms: D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D006386:Hemagglutination Tests; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D012397:Rosette Formation
chemical_list: D008937:Mitomycins; D010835:Phytohemagglutinins; D003609:Dactinomycin; D011691:Puromycin; D016685:Mitomycin; D012313:RNA; D004247:DNA