Induction of IgE receptors on human lymphocytes.
A mouse myeloma IgE protein was cross-linked with dimethylsuberimidate to induce polymerization and incubated with normal human peripheral blood T lymphocytes in tissue culture. After 24 hours at 37 degrees C approximately 30% of the cells formed rosettes with IgE sensitized red cells as compared with much lower levels in the controls. A variety of controls established that the cellular receptors involved in rosette formation were specific for IgE. Monomeric mouse IgE failed to induce a response.
D000328:Adult; D000818:Animals; D000939:Epitopes; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D012397:Rosette Formation
D000939:Epitopes; D017455:Receptors, IgE; D011971:Receptors, Immunologic