Fluorimetric determination of biogenic 5-hydroxy- and 5-methoxyindoles by high-performance liquid chromatography using perchloric acid as post-column reagent.
A method for the determination of biogenic 5-hydroxy- and 5-methoxyindoles using high-performance liquid chromatography with fluorescence detection was developed. The specific fluorescence of these indoles induced by perchloric acid was utilized as the post-column detection system. The determination limits were 3 pmol for 5-hydroxy-L-tryptophan and 5 pmol for serotonin (5-HT) and 5-hydroxyindole-3-acetic acid (HIAA). The method was applied to the determination of 5-HT and HIAA in rat brain tissues using 5-hydroxygramine as the internal standard.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D007211:Indoles / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis; D013050:Spectrometry, Fluorescence
D007211:Indoles; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid