Elimination of Mycoplasma orale from the human hepatoma cell line, PLC/PRF/5.
We have tried without success to eliminate M. orale from a human hepatoma line, PLC/PRF/5, using five different methods. We report here the successful elimination of the contamination by a modification of the technique of Marcus et al. [1] using 5-bromodeoxy-uridine (BrdU) instead of 5-bromo-uracil (5-BrUra) and light. We believe that this method may prove useful when rare and valuable cell lines carry the more common mycoplasma contaminant M. orale.
D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002470:Cell Survival; D003584:Cytological Techniques; D006801:Humans; D007621:Karyotyping; D008027:Light; D008113:Liver Neoplasms; D009154:Mutation; D009174:Mycoplasma / Q000254:growth & development*
D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole