Substance P: localization, concentration and release in cerebral arteries, choroid plexus and dura mater.
Substance P-like immunoreactivity (SPLI) was studied by immunocytochemistry and radioimmunoassay in the cerebral arteries, choroid plexus and dura mater of the guinea-pig, rabbit, cat and man. The highest concentrations were found in cerebral blood vessels: 6.1 +/- 2.3 pmol/g (guinea-pig), 9.0 +/- 1.1 pmol/g (rabbit), 7.1 +/- 0.4 pmol/g (cat), and 2.4 +/- 0.9 pmol/g (man). Lower levels were obtained in the choroid plexus and dura mater. The distribution of substance P (SP)-immunoreactive nerve fibres found in various regions of the guinea-pig correlated well with the amount of SPLI measured. Sympathectomy did not alter the concentration of SPLI in the dura mater or in cerebral blood vessels. Electrical field stimulation or 124 mM potassium enhanced the spontaneous efflux of SPLI by 10 and 20%, respectively, from superfused pial arteries in vitro. These data are in support of a functional role of perivascular SP within the cranial circulation.
D000818:Animals; D002415:Cats; D002536:Cerebral Arteries / Q000032:analysis*; D002831:Choroid Plexus / Q000032:analysis*; D004388:Dura Mater / Q000032:analysis*; D006168:Guinea Pigs; D006801:Humans; D009412:Nerve Fibers / Q000032:analysis; D011817:Rabbits; D013373:Substance P / Q000032:analysis* / Q000378:metabolism / Q000502:physiology
D013373:Substance P