The use of lanthanum chloride as a marker for intercellular junctions in rat exocrine pancreas.
A simple technique of perfusion and immersion of tissue in fixative containing lanthanum chloride as an extracellular tracer is described. In addition to functioning as a tracer, the lanthanum chloride appears to enhance electron staining. In rat exocrine pancreas, intercellular spaces between exocrine and centroductular cells were outlined clearly be electron dense material and, at cellular interfaces, spot desmosomes, gap junctions, and tight junctions were demonstrated. The technique proved simple and effective and should prove useful in studies of epithelial and other tissues.
D000818:Animals; D007365:Intercellular Junctions / Q000648:ultrastructure*; D007515:Islets of Langerhans / Q000648:ultrastructure*; D007811:Lanthanum; D051381:Rats; D013194:Staining and Labeling
C028521:lanthanum chloride; D007811:Lanthanum