Cation exchange--a common mechanism in the storage and release of biogenic amines stored in granules (vesicles)? I. Comparative studies on the uptake of sodium and biogenic amines by the weak cation (carboxyl) exchangers Amberlite IRC-50 and Sephadex C-50 and by biogenic (granule-enriched) materials in vitro.
Studies on the uptake and storage of sodium and biogenic amines (phenylethylamine, noradrenaline, histamine) by two weak cation-exchangers, IRC-50 and Sephadex C-50, and by biogenic granule-enriched preparations demonstrated that the synthetic and biogenic materials had several common characteristics. They showed similar concentration- and pH-dependence and fitted the same cation-exchange and receptor-binding equations. The observations were taken to support the view that the matrices of amine-storing granules have the properties of weak cation-exchangers, with carboxyls as the cation-binding groups.
D000818:Animals; D000837:Anion Exchange Resins / Q000494:pharmacology; D001679:Biogenic Amines / Q000378:metabolism*; D002411:Cation Exchange Resins / Q000494:pharmacology*; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism*; D003911:Dextrans / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins / Q000494:pharmacology*; D008407:Mast Cells / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D010627:Phenethylamines / Q000378:metabolism; D012117:Resins, Synthetic; D012964:Sodium / Q000378:metabolism*
D000837:Anion Exchange Resins; D001679:Biogenic Amines; D002411:Cation Exchange Resins; D003911:Dextrans; D007475:Ion Exchange Resins; D010627:Phenethylamines; D012117:Resins, Synthetic; D006632:Histamine; C024946:Amberlite IRC50; C025614:sephadex; D012964:Sodium; D009638:Norepinephrine