Localization of nodulation gene on the chromosome of Rhizobium meliloti.
Using N-methyl-N'-nitro-N-nitrosoguanidine mutant RM54 of Rhizobium meliloti L5-30 defective in the nodulation process (Nod-) and in the biosynthesis of adenine was obtained. Nod- phenotype of this mutant was not caused by the auxotrophic mutation. The nod gene is located on the chromosome. The wild type strain of R. meliloti and Nod- mutant RM54 harbour two indigenous plasmids having a molecular weight of 90 Mdal and about 300 Mdal.
D000225:Adenine / Q000096:biosynthesis; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005798:Genes, Bacterial; D000455:Medicago sativa / Q000382:microbiology*; D009154:Mutation; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology; D013559:Symbiosis; D014161:Transduction, Genetic
D000225:Adenine