Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate.
Poly-beta-hydroxybutyrate granules exhibited a strong orange fluorescence when stained with Nile blue A. Heat-fixed cells were treated with 1% Nile blue A for 10 min and were observed at an excitation wavelength of 460 nm. Glycogen and polyphosphate did not stain. Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B.
D001395:Azotobacter / Q000032:analysis; D001410:Bacillus megaterium / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000032:analysis; D005456:Fluorescent Dyes; D006885:Hydroxybutyrates / Q000032:analysis*; D010078:Oxazines; D011091:Polyesters; D011108:Polymers / Q000032:analysis*; D013194:Staining and Labeling; D000094122:Polyhydroxybutyrates
D005456:Fluorescent Dyes; D006885:Hydroxybutyrates; D010078:Oxazines; D011091:Polyesters; D011108:Polymers; C008619:Nile Blue; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates