The ultraviolet sensitivity of Cockayne syndrome cells is not a consequence of reduced cellular NAD content.
Cells from individuals with Cockayne syndrome (CS) are hypersensitive to the lethal effects of ultraviolet light (uv) and show a number of abnormal biochemical responses following uv-irradiation. Fujiwara et al. recently reported that the NAD contents of CS fibroblasts were lower than those of normal fibroblasts, and that addition of NAD to the cellular growth medium rectified most of the abnormal responses of CS cells to uv-irradiation. In our experiments, however, the cellular NAD contents of normal and CS fibroblasts were similar, and addition of NAD to the growth medium had no effect on the hypersensitivity of CS cells to uv-irradiation, nor did it restore the inability of CS cells to recover normal rates of DNA or RNA synthesis following uv-irradiation.
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D003057:Cockayne Syndrome / Q000150:complications / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004392:Dwarfism / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008297:Male; D009243:NAD / Q000378:metabolism*; D010787:Photosensitivity Disorders / Q000209:etiology / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays / Q000009:adverse effects*
D009243:NAD; D012313:RNA; D004247:DNA