The 5'-terminal ribonucleotide of an in vitro ColE1 transcript is not complementary to the DNA template.
A set of overlapping promoters in ColE1 DNA is identified using DNA and RNA sequencing methods. These promoters initiate a divergent pattern of in vitro transcription from a region near the colicin E1 promoter. A comparison of the 5'-terminal RNA sequences of the in vitro transcripts with the DNA sequence of the template indicates that one transcript initiates with a ribonucleotide (pppG) which is not complementary to the DNA template (a thymidylate residue is present on the coding strand at the designated transcription start point). A structural and functional counterpart for this phenomenon is suggested to exist near the opposite end of the colicin E1 gene.
D003086:Bacteriocin Plasmids; D001483:Base Sequence; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D012329:RNA, Bacterial / Q000235:genetics; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial