Monoclonal antibodies to HLA recognize monomorphic and polymorphic epitopes on BoLA.
Fourteen monoclonal antibodies recognizing monomorphic and polymorphic epitopes on class I and class II antigens of the human MHC have been assayed on lymphocytes of a panel of 20-150 BoLA typed bovine animals from 12 different breeds. Some monomorphic antibodies cross-reacted and others did not. Two polymorphic monoclonal antibodies in man recognize a polymorphism in cows that follows allospecificities (BoLA-w3, w9) already described. Immunoprecipitation experiments with monomorphic anti-B2m and anti-HLA-DR monoclonal antibodies have shown that these cross-reactions concern BoLA antigens. They also revealed that Ia-like antigens in cattle present the same two chain features characterized in other species.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008285:Major Histocompatibility Complex; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens