title: Radioimmunoassay of serum prostatic acid phosphatase after prostatic massage.
abstract: The effect of prostatic massage on the concentration of prostatic acid phosphatase (PAP) in blood serum as determined by radioimmunoassay (RIA) was compared with that determined by a standard enzymatic assay (EA). Serum was drawn from 24 men before prostatic massage and after--at specified intervals, up to twenty-four hours. Three of these men were young, normal controls; 10 had biopsy-proved prostate cancer (CA); 11 had histologically confirmed benign prostatic hyperplasia (BPH). After prostatic massage, 3 of the 10 CA patients (30%) had elevation of PAP as determined by EA and 4 of the 11 BPH patients (36%) as determined by RIA. None of the controls showed elevated levels of PAP by either assay. In all patients elevated levels of PAP by both assays had returned to normal twenty-four hours after massage. It was concluded that serum for PAP testing by either assay method should be drawn before or twenty-four hours after rectal examination to prevent false positive results and the need for retesting.
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D000328:Adult; D001706:Biopsy; D004796:Clinical Enzyme Tests; D005189:False Positive Reactions; D006801:Humans; D008297:Male; D008405:Massage; D010808:Physical Examination; D011467:Prostate / Q000201:enzymology; D011470:Prostatic Hyperplasia / Q000201:enzymology / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000473:pathology; D011863:Radioimmunoassay; D013997:Time Factors
chemical_list: D000135:Acid Phosphatase