Regulation of poly(ADP-ribose) transferase activity by 2',5'-oligoadenylates.
pppA2'pA2'pA appears to be a potent natural noncompetitive inhibitor of poly (ADP-ribose) transferase activity in the histone dependent reaction of ADP-ribosylation with Ki=5 microM. Moreover, it is a noncompetitive inhibitor of the Mg2+ dependent reaction of autoADPRT-ribosylation with Ki=20 microM. The activity of ADPRT falls down abruptly both in the cytoplasm and nuclei of mouse L-cells treated with interferon. In contrast, the activities of 2',5'-oligo (A) polymerase and 2'-phosphodiesterase remain virtually unchanged after the treatment with ADPRT preparation. The regulation of ADPRT activity and active form of ADPRT by 2',5-oligoadenylates is presumed to be one of the factors responsible for inducing the antiviral and/or antiproliferative effects of interferon.
D000227:Adenine Nucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004722:Endoribonucleases / Q000378:metabolism; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology; D007700:Kinetics; D007739:L Cells / Q000201:enzymology; D008297:Male; D051379:Mice; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D013737:Testis / Q000201:enzymology
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C023505:2',5'-oligoadenylate; D007372:Interferons; D011065:Poly(ADP-ribose) Polymerases; C033547:2'-5'-oligo(A)-dependent endonuclease; D004722:Endoribonucleases; D010727:Phosphoric Diester Hydrolases; D009244:NAD+ Nucleosidase