A simple method of preparing smears from bloody effusions for cytodiagnosis.
A simple method of preparing smears from bloody effusions by a microhematocrit technique is described. The nucleated cells in smears prepared by this method were located in the center of the smear in a single layer. They were not obscured by either proteinaceous precipitates or erythrocytes, and their nuclear morphology could be easily appreciated. A large number of cells with excellent morphologic preservation could thus be examined in a short period of time. These preparations facilitated the detection of abnormal cells in bloody serous effusions.
D001769:Blood; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D005122:Exudates and Transudates / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000473:pathology; D010490:Pericardial Effusion / Q000097:blood / Q000175:diagnosis; D013194:Staining and Labeling / Q000379:methods