[Isolation of the RNA-poly A+ fraction from mitochondria of Drosophila melanogaster embryos. Incorporation of methionine-35S into newly-synthesized proteins in whole and lysed mitochondria].
The poly A+ RNA fraction of embryos mitochondria of Drosophila melanogaster was prepared by fractionation by oligo dT-cellulose chromatography. It accounted for 5% of total insoluble RNA. 15 bands were identified by electrophoresis, from 500 to 2,000 base pairs. This poly A+ RNA fraction hybridizes with mitochondrial DNA clones. It contains mitochondrial transcripts: messenger RNA and the large ribosomal RNA. The sum of the transcripts (29 kb) exceed the coding capacities of the mitochondrial DNA. Some of the transcripts may be precursors, greater in size, of the mRNA corresponding to a gene. Our results are compared to those previously published (9, 11, 12). The relationship between mitochondrial transcripts and proteins was studied in whole and lysed isolated mitochondria. In the former, incubated in the presence of 35S-methionine, 20 to 30 radioactive bands were identified (M. Wt. 100 to 16 kd). This incorporation was totally inhibited by chloramphenicol. When the mitochondria were opened in the presence of detergent (lysis). 35S-methionine incorporation was markedly enhanced. In all cases the number of proteins identified was greater than the number of potential genes (12). This finding is discussed.
D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D008715:Methionine / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D011061:Poly A / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D011506:Proteins; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D011061:Poly A; D012313:RNA; D008715:Methionine
Isolement de la fraction d'ARN-poly A+ des mitochondries d'embryons de Drosophila melanogaster. Incorporation de méthionine-35S dans les protéines néosynthétisées dans les mitochondries entières et lysées.