[Antigenic determinant study of the hemagglutinin in the composition of influenza viruses A subtype H1N1 by an indirect solid-phase radioimmunological analytical method].
A comparative study of antigenic determinants of influenza A virus, subtype H1N1, hemagglutinins isolated in 1947-1953 and in 1977-1979 was carried out. The competitive capacity of 8 virus strains for antibody of 4 hyperimmune antisera to A/Fm/1/47, A/Moscow/Pan/52, A/USSR/090/77, and A/Brazil/11/78 viruses was studied by solid-phase radioimmunoassay (SPRIA) in two modifications: in the homologous and heterologous systems. The analysis of competition in the heterologous system permits one to study isolated subpopulations of antibody homologous to viruses fixed on the solid phase. The combination of both modifications allows investigations of a wider spectrum of antibodies than those possible in the homologous system alone. The results of the study showed the antigenic determinants of hemagglutinin exposed on the surface of particles of different influenza A virus strains to differ in their capacity to stimulate antibody synthesis. The analysis of the time course of changes in the antigenic properties of hemagglutinin regions of similar structure in various viral strains may facilitate prognosing of evolution of virus strains of the same subtype.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D006389:Hemagglutinins, Viral / Q000032:analysis*; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral
Izuchenie antigennykh determinant gemaggliutinina v sostave virusov grippa A podtipa H1N1 metodom nepriamogo tverdofazovogo radioimmunologicheskogo analiza.