title: Interferon and natural killing of human lymphoma cell lines after induction of the Epstein Barr viral cycle by superinfection.
abstract: Interferon (IFN) production during natural killer (NK) cell assays with Raji, an EBV-carrying human lymphoma-derived cell line, was studied to determine whether IFN generated by effectors in vitro acted in target cell lysis. In 4-hr tests, Raji is insensitive to NK but becomes susceptible after superinfection with the P3HR-1 strain of EBV. IFN was not detectable by bioassay in supernatants from 4-hr assays, and the addition of antibody to IFN did not prevent the lysis of the superinfected Raji cells. In 18-hr tests the NK sensitivity of the superinfected Raji cells was markedly elevated, and a percent of the normal Raji cells was also killed. IFN alpha was found in supernatants from 18-hr tests. Antibody to IFN alpha markedly reduced the killing of superinfected Raji and slightly reduced cytotoxicity against control Raji in 18-hr tests. Taken together these results indicate that what is referred to as natural killing has IFN-related and IFN-nonrelated components.
mesh_terms: D000906:Antibodies / Q000502:physiology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006566:Herpesviridae Infections / Q000150:complications / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000254:growth & development; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007372:Interferons / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*; D008223:Lymphoma / Q000150:complications / Q000276:immunology*; D014775:Virus Activation
chemical_list: D000906:Antibodies; D007372:Interferons