Ion-pair extraction of bile acids with Lipidex gel.
A new method for the extraction of bile acids from aqueous solutions, urine, plasma, and bile is described. A buffered solution of decyltrimethylammonium bromide is added to the sample to give a 0.03 M concentration of the counter-ion. The mixture is passed through a bed of Lipidex 1000, which is then washed with the buffered solution of counter-ion followed by water. The decyltrimethylammonium salts of bile acids are sorbed by the Lipidex and are eluted with methanol. Recoveries of unconjugated, taurine- and glycine-conjugated, sulfated, and glucuronidated bile acids are close to 100%. Unconjugated bile acids can also be quantitatively extracted from aqueous solutions and urine after acidification with acetic acid.
D000818:Animals; D001646:Bile / Q000032:analysis; D001647:Bile Acids and Salts / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D003911:Dextrans; D005782:Gels; D007475:Ion Exchange Resins; D051381:Rats
D001647:Bile Acids and Salts; D003911:Dextrans; D005782:Gels; D007475:Ion Exchange Resins; C010599:lipidex