Thrombin-induced degranulation of cultured bone marrow-derived mast cells.
This study was undertaken to determine if a plasma protease such as thrombin, a highly specific procoagulant enzyme that has numerous effects on platelets, endothelial cells, and smooth muscle cells, could mediate mast cell activation processes. Our results indicate that at near physiologic levels, thrombin can rapidly trigger mast cell degranulation without activating the 5-lipoxygenase system.
D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology; D001854:Bone Marrow Cells; D002417:Cattle; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D006629:Hirudins / Q000494:pharmacology; D006636:Histamine Release; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D013379:Substrate Specificity; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases
D006629:Hirudins; D000990:Antithrombin III; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases; D013917:Thrombin