Expression of beta 2-microglobulin on preimplantation pig embryos.
The expression of beta-2 microglobulin (beta 2m), a protein associated with the histocompatibility antigens of the pig complex (SLA), was studied in preimplantation embryos between the segmentation stage and the beginning of elongation (day 12). The antigen was visualized at the ultrastructural level by immunocytochemical techniques using peroxidase or colloidal gold particles as labels. beta 2m expression appeared to parallel trophoblast differentiation. No positive reaction was obtained before the early blastocyst stage. From this stage on, labelling was observed on the apical surface of the trophectoderm cells.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001609:Beta-Globulins / Q000276:immunology*; D047108:Embryonic Development; D005260:Female; D006649:Histocompatibility Antigens; D007106:Immune Sera / Q000494:pharmacology; D011247:Pregnancy; D011817:Rabbits; D013552:Swine; D014327:Trophoblasts / Q000276:immunology* / Q000648:ultrastructure; D001613:beta 2-Microglobulin / Q000032:analysis / Q000276:immunology*
D000941:Antigens; D001609:Beta-Globulins; D006649:Histocompatibility Antigens; D007106:Immune Sera; D001613:beta 2-Microglobulin