Transformation of LMTK- cells with purified HLA class I genes--IV. A determinant on beta 2-microglobulin is controlled by HLA heavy chain in a mouse-human hybrid complex: a biochemical analysis.
Transformation of LMTK- murine fibroblast cells with purified HLA class I heavy chain genes resulted in the expression of serologically detectable HLA-A3 molecules. Surprisingly, such cells also react with a murine monoclonal antibody specific for a serological determinant notably not expressed by murine but by human beta 2-microglobulin. The human HLA molecules expressed by the transformed cells were characterized on two-dimensional gels. The heavy chain was shown to be associated with a murine beta 2-microglobulin molecule, which could be distinguished from human beta 2-microglobulin by its higher isoelectric point. This heterodimer molecule was immunoprecipitated with the mouse anti-human beta 2-microglobulin monoclonal antibody showing that indeed the complex of mouse beta 2-microglobulin and human heavy chain expresses a human beta 2-microglobulin determinant.
D000818:Animals; D002460:Cell Line; D004587:Electrophoresis, Agar Gel; D000939:Epitopes; D005347:Fibroblasts; D005796:Genes; D006680:HLA Antigens / Q000235:genetics*; D015791:HLA-A3 Antigen; D006801:Humans; D006822:Hybrid Cells; D007525:Isoelectric Focusing; D051379:Mice; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D014170:Transformation, Genetic; D001613:beta 2-Microglobulin / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D015791:HLA-A3 Antigen; D001613:beta 2-Microglobulin