The nucleotide sequence of cDNA coding for preproinsulin from the primate Macaca fascicularis.
DNA complementary to preproinsulin messenger RNA from the primate Macaca fascicularis has been cloned into the PstI endonuclease site of the plasmid pBR322. One clone contains the entire preproinsulin coding region as well as 59 nucleotides of the 5'-untranslated region. The results predict an amino acid sequence for the Macaca fascicularis preproinsulin and establish for the first time that the primary structures of human and primate insulins are identical. The two amino acid exchanges between human and primate preproinsulins are restricted to the pre- and the C-peptide, respectively.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007328:Insulin; D008252:Macaca fascicularis; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D011384:Proinsulin / Q000235:genetics*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; D011061:Poly A; C013737:preproinsulin; D012313:RNA; D004247:DNA; D011384:Proinsulin