[Synthesis of nucleic RNA in hydroxythiamine-induced vitamin B1 deficiency].
24 hours after administration of hydroxythiamine (vitamin B1 antimetabolite) in a dose of 40 mg per 100 g of body weight, the incorporation of (2(14)C)-orotic acid and (methyl-14C)-methionine into total nuclear RNA decreases. The content of RNA and the specific radioactivity of cytoplasmic UTP and methionine pools remain unchanged. Thermal phenol fractionation of nuclear RNA reveals a decrease in a predominant synthesis of nucleolar RNA, while that of heterogeneous nuclear RNA, tRNA, and 5S-RNA remains unaffected.
D000818:Animals; D008099:Liver / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism; D009963:Orotic Acid / Q000378:metabolism; D010119:Oxythiamine / Q000633:toxicity; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis; D051381:Rats; D013832:Thiamine Deficiency / Q000139:chemically induced / Q000378:metabolism*
D010119:Oxythiamine; D009963:Orotic Acid; D012313:RNA; D012343:RNA, Transfer; D008715:Methionine
Sintez iadernykh RNK pri vyzvannoĭ oksitiaminom nedostatochnosti vitamina B1.