Glycoproteins of axonal transport. Interaction with heparin.
Rapidly transported fucose-containing glycoproteins, in axons and nerve terminals, from retinal ganglion cells of the rabbit have been characterized via affinity chromatography on a new fucose-specific lectin from Aleuria aurantia. It was found that a relatively small number of highly labelled transported polypeptides did not bind to heparin-Sepharose. These polypeptides also showed a low affinity for the dye Coomassie brilliant blue following sodium dodecyl sulphate electrophoresis.
D000818:Animals; D001370:Axonal Transport; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000302:isolation & purification*; D006493:Heparin; D037102:Lectins; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009474:Neurons / Q000032:analysis; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000032:analysis*
D006023:Glycoproteins; D037102:Lectins; D009419:Nerve Tissue Proteins; C027112:fucose-binding lectin; D006493:Heparin