title: Human newborn autologous mixed lymphocyte response: frequency and phenotype of responders and xenoantigen specificity.
abstract: The response of human newborn lymphocytes in autologous mixed lymphocyte culture was examined for specificity (by restimulation), responder cell phenotype, and responder cell frequency. When the newborn T cells were separated from non-T cells by rosetting with sheep erythrocytes (E) in fetal calf serum (FCS), approximately 1:20,000 T cells proliferated. These responders had specificity for E + FCS, were T4+, and were self-restricted. Significant numbers of responder T cells were not found when newborn T and non-T cells were separated by nylon wool. Responses in parallel autologous cultures of adult T cells showed that 1) adults had a higher frequency than newborns of E + FCS specific responders, 2) evidence for self specificity was lacking in restimulated cultures, and 3) occasional responses to antigen on the surface of monocytes could not be excluded.
mesh_terms: D000328:Adult; D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology; D000939:Epitopes; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D010641:Phenotype; D012756:Sheep; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology
chemical_list: D015478:Antigens, Heterophile; D001789:Blood Group Antigens; D000939:Epitopes