title: Chemotactic macrophage subpopulations defined by macrophage chemotactic factors from delayed hypersensitivity reaction sites.
abstract: The chemotactic specificity of ia-positive and -negative macrophages was studied by using three macrophage chemotactic factors (MCF), -a, -b, and -c, isolated from delayed hypersensitivity reaction (DHR) skin sites in guinea pigs. Listeria-elicited macrophages migrated toward MCF-a, -b, and -c. The chemotactic responses suggested responsive subpopulations to MCF. The electronic programmable individual cell sorter (EPICS) was used to separate macrophages with anti-la monoclonal antibodies. Ia-positive subpopulations responded to MCF-c, although they did not migrate toward MCF-a and -b. In contrast, Ia-negative subpopulations migrated toward MCF-a and -b, but not toward MCF-c. Furthermore, MCF-c attracted Ia-positive macrophages, whereas MCF-a and -b were Ia-negative in vitro; MCF did not induce Ia-negative macrophages to express surface Ia-antigens in vitro. MCF-c was able to produce massive Ia-positive macrophage accumulations when injected i.p., whereas MCF-a accumulated Ia-negative macrophages. The data suggest that MCF-a and -b, which mediate initial macrophage reactions, attract Ia-negative macrophages, and that MCF-c, which mediates predominant macrophage reactions, attract Ia-positive macrophages in the DHR.
mesh_terms: D000818:Animals; D002469:Cell Separation; D002630:Chemotactic Factors / Q000008:administration & dosage / Q000276:immunology / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D008264:Macrophages / Q000145:classification* / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H
chemical_list: D002630:Chemotactic Factors; D000939:Epitopes; D000949:Histocompatibility Antigens Class II