Visualization of both peroxidase and acid phosphatase isozymes from flax (Linum) on the same gels.
The staining of both peroxidases (PERs) and acid phosphatases (APs) following electrophoresis on acrylamide gels is described. Scanning doubly stained gels at appropriate wavelengths reveals individual isozyme bands for both enzyme systems, giving essentially identical results to those obtained from separately run and stained gels. Collection of relative mobility (Rm) data is unaffected by such tandem staining. Few complications arise from overlapping PER and AP bands, or interactions between the two staining methods and PER or AP isozymes. Even if repeat scans of a gel at different wavelengths are needed to retrieve all isozyme information, dual staining provides distinct savings in time and sample material.
D000135:Acid Phosphatase / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006138:Guaiac; D007527:Isoenzymes / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D010944:Plants / Q000201:enzymology*; D013053:Spectrophotometry; D013194:Staining and Labeling
D007527:Isoenzymes; D006138:Guaiac; D010544:Peroxidases; D000135:Acid Phosphatase