Rapid purification of fluorescent enzymes by ultrafiltration.
In order to expedite the preparation of fluorescently tagged enzymes for histo/cytochemistry, a previously developed method employing gel column purification was compared with a more rapid modern technique using the Millipore Immersible CX-ultrafilter. Microscopic evaluation of the resulting conjugates showed comparable products. Much time and effort is saved using the new technique.
D002480:Cellulase / Q000302:isolation & purification*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005453:Fluorescence; D005456:Fluorescent Dyes; D009113:Muramidase / Q000302:isolation & purification*; D013194:Staining and Labeling; D013861:Thiocyanates; D014462:Ultrafiltration
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D009113:Muramidase; D002480:Cellulase; D016650:Fluorescein-5-isothiocyanate