Precise localization of several covalent RNA-RNA cross-link in Escherichia coli 16S RNA.
The RNA-RNA cross-linking reagent N-acetyl-N'-(p-glyoxylyl-benzoyl)cystamine, which reacts via its glyoxal residue with guanines not involved in G X C base pairs, has been used to introduce reversible RNA-RNA cross-links into Escherichia coli 16S rRNA. A two-dimensional gel method has been devised for the separation of the cross-linked oligonucleotides, and the precise location of guanines involved in four of these cross-links has been determined by sequencing the oligonucleotides. One cross-link involves guanosines 1315 and 1360 situated in two hairpin end loops of domain III. The other cross-links involves pairs of guanosine situated within the same hairpin end loops.
D001482:Base Composition; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial; D012335:RNA, Ribosomal
D003432:Cross-Linking Reagents; D012329:RNA, Bacterial; D012335:RNA, Ribosomal