Immunoelectron microscopic localization of rabies virus antigen in central nervous system and peripheral tissue using low-temperature embedding and protein A-gold.
A protein A-gold technique was used in conjunction with low temperature embedding to visualize ultrastructurally mature virions and sites of viral replication in the brains of rabies virus-infected mice after peripheral inoculation of virus. The association of viral profiles and gold particles with synaptic membranes, microtubules and rough endoplasmic reticulum suggested a mechanism of rabies virus transport within the central nervous system. Early interactions of inflammatory cells with the virus inoculum were characterized by phagocytosis by non-degranulating neutrophils and mononuclear cells.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002540:Cerebral Cortex / Q000382:microbiology*; D003080:Cold Temperature; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D009132:Muscles / Q000382:microbiology*; D009474:Neurons / Q000648:ultrastructure; D011820:Rabies virus / Q000032:analysis / Q000276:immunology*; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling; D014771:Virion / Q000648:ultrastructure
D000956:Antigens, Viral