Interactions of dopamine and the release of [3H]-taurine and [3H]-glycine from the isolated retina of the rat.
1 The dose-related, calcium-dependent, potassium-stimulated release of preloaded [(3)H]-dopamine from the superfused rat retina has been demonstrated.2 A high-affinity uptake system for dopamine exists in rat retina in vitro; K(m) value was calculated as 1.89 muM, V(max) value as 1.4 nmol g(-1) tissue h(-1).3 Dopamine (0.8 and 4 mM) inhibited the spontaneous release of [(3)H]-glycine from retina, and in the case of 0.8 mM dopamine this inhibitory effect was antagonized by 10 muM (+)-butaclamol but not by 10 muM (-)-butaclamol.4 The potassium-evoked (25 mM) release of [(3)H]-glycine from rat retina was similarly inhibited by dopamine (0.4-4 mM) in a dose-related manner when added to the superfusate with the potassium. The effect of 0.8 mM dopamine was antagonized by 10 muM (+)-butaclamol but not by 10 muM (-)-butaclamol.5 Dopamine (4 mM) significantly reduced the spontaneous release of [(3)H]-taurine from rat retina.6 The potassium-stimulated (25 mM) release of [(3)H]-taurine occurred after the cessation of the depolarizing stimulus. This delayed release of [(3)H]-taurine was unaffected if dopamine was applied to the superfusate at the same time as the potassium, but it was significantly reduced if dopamine (0.8 and 4 mM) was applied after the depolarizing stimulus had been removed and during the actual amino acid release phase.7 The inhibition of K(+)-stimulated (25 mM) delayed release of [(3)H]-taurine by applying dopamine (0.8 mM) after the depolarizing stimulus was blocked by 10 muM (+)-butaclamol but not by 10 muM (-)-butaclamol.8 The results are discussed with respect to the possible neurotransmitter role for dopamine within the rat retina, and its possible interaction with glycine and taurine.
D000818:Animals; D002069:Butaclamol / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D005260:Female; D005998:Glycine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000378:metabolism*; D013237:Stereoisomerism; D013654:Taurine / Q000378:metabolism*
D013654:Taurine; D011189:Potassium Chloride; D002069:Butaclamol; D011188:Potassium; D005998:Glycine; D004298:Dopamine