title: Plasmodium chabaudi antigens synthesized in an mRNA-dependent cell-free translation system.
abstract: Intact RNAs were isolated from Plasmodium chabaudi growing synchronously in mice at different stages of the erythrocytic cycle. Translation of the mRNAs using rabbit reticulocyte lysate showed stage-specific patterns comparable to those observed in vivo. Many antigens of P. chabaudi are produced by translation in the rabbit reticulocyte lysate. Some other antigens are predominantly synthesized at a particular stage, indicating that the mRNA populations differ from one stage to another.
mesh_terms: D000818:Animals; D000941:Antigens / Q000096:biosynthesis*; D002474:Cell-Free System; D005260:Female; D005815:Genetic Code; D051379:Mice; D010961:Plasmodium / Q000276:immunology*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics*; D012335:RNA, Ribosomal / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis
chemical_list: D000941:Antigens; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012313:RNA