Monoclonal suppressor T-cell factor displaying V H restriction and fine antigenic specificity.
The production of stable T-cell clones is essential for the study of T-cell-derived, specific immunoregulatory products and of specific T-cell receptors. T-cell clones have been established by radiation leukaemia virus (RadLV)-induced transformation of suppressor T lymphocytes specific for hen egg white lysozyme (HEL). We report here that culture supernatant obtained from these T-cell clones can, when injected into mice, specifically suppress the anti-HEL antibody response. This monoclonal T-cell product suppresses the antibody response induced by HEL and human lysozyme, but not that induced by ring-necked pheasant egg white lysozyme (REL), thus displaying fine antigenic specificity probably restricted to an epitope involving phenylalanine at amino acid residue 3, present in the N-terminal region of HEL and shared by human lysozyme but absent in REL. The suppression induced by this monoclonal T-cell product is restricted by both H-2 and Igh-1 genes whereas anti-HEL antibodies bearing a predominant idiotype are induced in all mice strains tested, irrespective of their H-2 haplotype or Igh-1 allotype.
D000818:Animals; D001717:Birds; D002460:Cell Line; D002999:Clone Cells; D000939:Epitopes / Q000032:analysis*; D006183:H-2 Antigens / Q000276:immunology; D006801:Humans; D007165:Immunosuppression Therapy; D008222:Lymphokines / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009113:Muramidase / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D013491:Suppressor Factors, Immunologic
D000939:Epitopes; D006183:H-2 Antigens; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D009113:Muramidase