Synergistic effect of A23187 and a phorbol ester on amylase secretion from rabbit pancreatic acini.
The combination of the ionophore A23187 and the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) stimulates amylase secretion from rabbit pancreatic acini up to a level equal to, or slightly higher than when carbachol is used as stimulant. Each of the two compounds alone gives only a minor stimulation. This synergistic effect of A23187 and TPA supports a role of protein kinase C in pancreatic enzyme secretion.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D010704:Phorbols / Q000494:pharmacology*; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D010704:Phorbols; D000001:Calcimycin; D002217:Carbachol; D000681:Amylases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium