Isolation and initial characterization of residual nuclear structures from yeast.
Residual nuclear structures have previously been isolated from a wide range of eukaryotic organisms. When nuclei are isolated from Saccharomyces cerevisiae and then treated with 1.95 M NaCl and DNase I, sedimentable residual structures are obtained similar in several respects to structures isolated from organisms previously studied. These yeast residual nuclear structures retain less than 7% of nuclear DNA, less than 17% of nuclear RNA and less than 50% of nuclear proteins. Electron microscopy suggests that these structures are derived from the nuclear interior and are composed of a sparse fibrogranular network. Replicating DNA is preferentially bound to these yeast residual nuclear structures, just as it is to residual nuclear structures from other organisms.
D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000648:ultrastructure*; D012972:Sodium Hydroxide
D011506:Proteins; D012972:Sodium Hydroxide; D012313:RNA; D004247:DNA