Simplified determination of serotonin in plasma by liquid chromatography with electrochemical detection.
This simplified determination of serotonin in human plasma involves "high-performance" liquid chromatography with electrochemical detection. Deproteinated samples of platelet-poor plasma are injected directly into the chromatograph without preliminary chromatographic purification. The limit of quantitative detection of serotonin in plasma is 5 nmol/L (equivalent to 250 fmol injected). Intra- and inter-assay CVs ranged from 5.6% to 7.7% and 3.5% to 14.9%, respectively, for concentrations of serotonin from 0.02 to 10.0 mumol/L. Results agreed well with those by spectrofluorometry, both for plasma standards (r = 0.985, range 100 nmol/L to 100 mumol/L) and for pathological samples obtained from four carcinoid syndrome patients (r = 0.964). The method is also applicable to the concurrent measurement of 5-hydroxytryptophan and 5-hydroxyindoleacetic acid.
D006916:5-Hydroxytryptophan / Q000097:blood; D000293:Adolescent; D000328:Adult; D002395:Catecholamines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D008297:Male; D008303:Malignant Carcinoid Syndrome / Q000097:blood*; D008875:Middle Aged; D012701:Serotonin / Q000097:blood* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013223:Statistics as Topic
D002395:Catecholamines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006916:5-Hydroxytryptophan