title: Relationship between cytidine 5'diphosphocholine epitope density on ovalbumin and IgE antibody responses compared to other isotypes.
abstract: Ovalbumin was covalently substituted by 6, 18 and 20 residues of Cytidine 5'diphosphocholine per molecule. When injected into BALB/c mice, these products induced distinct immunogenic responses. Without any residue, or with six residues, ovalbumin induced hemagglutinating antibodies (1/256, 21 days after injection) and a response to the passive cutaneous anaphylactic reaction (1/400, 21 days after injection). By contrast, with 18 or 20 residues, ovalbumin brought about the appearance of only a weak titer of hemagglutinating antibodies (1/64, 21 days after injection); no response to the passive cutaneous anaphylactic reaction was observed. No anti-cytidine 5'diphosphocholine antibodies were detected by either reaction. Thus epitope density plays a major role in the IgE immune response to PC-OA.
mesh_terms: D000818:Animals; D002794:Choline / Q000031:analogs & derivatives*; D003566:Cytidine Diphosphate Choline / Q000276:immunology*; D000939:Epitopes; D006388:Hemagglutinins / Q000096:biosynthesis; D007073:Immunoglobulin E / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008968:Molecular Conformation; D010047:Ovalbumin / Q000276:immunology*; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000939:Epitopes; D006388:Hemagglutinins; D007073:Immunoglobulin E; D003566:Cytidine Diphosphate Choline; D010047:Ovalbumin; D002794:Choline