Activation of cyclic AMP-dependent protein kinase by VIP in blood mononuclear cells.
Vasoactive intestinal peptide stimulated cyclic AMP-dependent protein kinase activity in human blood mononuclear cells. The simultaneous presence of a phosphodiesterase inhibitor was required to elicit maximal activation. The apparent Ka value of half the maximal stimulation was about 60 pmol. Secretin exhibited a 170-times lower potency. Other peptides such as glucagon or insulin had no effect even at 1 microM.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000328:Adult; D004347:Drug Interactions; D004789:Enzyme Activation; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009000:Monocytes / Q000201:enzymology*; D011494:Protein Kinases / Q000097:blood*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D014660:Vasoactive Intestinal Peptide; D011494:Protein Kinases; D015056:1-Methyl-3-isobutylxanthine