Translation of mRNA from rat kidney following acute exposure to lead.
1. Two days after the administration of Pb2+ (0.54 mg/100 body wt) to rats, the kidneys were removed and homogenate fractions obtained. 2. The machinery for peptide synthesis exhibited increased activity in the kidney preparations from lead-treated rats. 3. The poly(A)+ RNA was obtained from the polysome fraction and translated in a mRNA-dependent reticulocyte lysate system. 4. The translatability of the poly(A)+ RNA was similar using the preparations obtained from control and lead-treated rats except for proteins of approx molecular weight of 50,000 which contained increased amounts [14C]leucine in the fractions derived from lead-treated rats. 5. The results are compared with those after acute Cd2+ toxicity where poly(A)+ RNA exhibited increased translatability for low molecular weight metallothioneins.
D000818:Animals; D007668:Kidney / Q000378:metabolism*; D007855:Lead Poisoning / Q000378:metabolism*; D008297:Male; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA