Isolation of guinea pig basophils using anti-leukocyte antibody and density gradient centrifugation on Percoll.
Peripheral blood basophils were isolated from guinea pigs using a 2-step procedure. Initial enrichment of basophils was achieved by treating leukocyte preparations obtained from whole blood with antibody to contaminating granulocytes and mononuclear cells. Basophil populations of 82% purity (mean; range 65-99%) were obtained by subsequent application of the antibody treated preparation to discontinuous Percoll gradients. The isolated basophils were viable and shown to be functional by histamine release upon stimulation with various secretogogues.
D000818:Animals; D001491:Basophils / Q000276:immunology* / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D005260:Female; D006168:Guinea Pigs; D006636:Histamine Release; D007106:Immune Sera / Q000494:pharmacology*; D007163:Immunosorbent Techniques; D007962:Leukocytes / Q000276:immunology; D011205:Povidone; D011817:Rabbits; D012822:Silicon Dioxide
D007106:Immune Sera; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone