A monoclonal antibody against nuclear lamina proteins reveals cell type-specificity in Xenopus laevis.
Immunofluorescence microscopy shows that the monoclonal murine antibody PKB8 stains the nuclear lamina of various somatic cells from vertebrates as diverse as mammals, birds and amphibia. It also decorates the nuclear periphery of oocytes from rat and chicken but does not react with spermatocytes, spermatids and spermatozoa. Immunoblotting experiments demonstrate reaction with lamina polypeptides A, B and C of rat, with lamina polypeptide A of chicken, and with lamina polypeptides LI and LII of erythrocytes of the frog, Xenopus laevis. Antibody PKB8 does, however, not bind, on blotted polypeptides and on sections through ovaries, to the pore complex-lamina polypeptide of Mr 68000 present in Xenopus oocytes. These results reveal the existence of a common antigenic determinant in all three lamina polypeptides of mammals, in one lamina polypeptide of chicken and in two amphibian lamina polypeptides. The immunological data also indicate that, in Xenopus laevis, pore complex-lamina polypeptides of somatic cells and oocytes are distinct. The Mr 68000 protein of Xenopus oocytes is also different from polypeptides LI and LII of somatic Xenopus cells by tryptic peptide mapping. The results suggest that nuclear pore complex-lamina polypeptides represent a family of related polypeptides containing regions highly conserved during evolution and that these polypeptides can be differentially expressed in cells of at least one species, Xenopus laevis.
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002460:Cell Line; D002642:Chick Embryo; D000939:Epitopes / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D034882:Lamins; D008099:Liver / Q000032:analysis; D007614:Macropodidae; D009698:Nucleoproteins / Q000032:analysis / Q000276:immunology*; D009865:Oocytes / Q000032:analysis; D010455:Peptides / Q000032:analysis; D051381:Rats; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D034882:Lamins; D009698:Nucleoproteins; D010455:Peptides