Platelet BTG release in vitro induced by mechanical and chemical stimulus: correlation with the aggregation curve parameters.
The in vitro release of B-thromboglobulin (BTG) from platelets after stirring the platelet rich plasma (PRP) 5 min (37 degrees C/1000 rpm) was studied in a group of 13 healthy subjects before and after 'in vivo' aspirin ingestion, 1 g/d for 3 d. A significant reduction in the platelet BTG release (31%) was observed after aspirin ingestion in comparison to the pre-aspirin values, suggesting that the BTG release by stirring is related to a platelet activation process. Small doses of collagen (0.625 microgram/ml) and thrombin (0.1 U/ml) always released platelet BTG, whether or not there was a visible platelet aggregation, suggesting that platelet activation is not necessarily followed by visible platelet aggregation. A significant correlation was found between the BTG released by stimulation with collagen (2.5 microgram/ml) and thrombin (0.2 U/ml) and the maximum velocity and intensity of the aggregation curve parameters.
D000328:Adult; D001241:Aspirin / Q000494:pharmacology; D001609:Beta-Globulins / Q000378:metabolism*; D003094:Collagen / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D010812:Physical Stimulation; D010974:Platelet Aggregation / Q000187:drug effects; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology; D001620:beta-Thromboglobulin / Q000378:metabolism*
D001609:Beta-Globulins; D001620:beta-Thromboglobulin; D003094:Collagen; D013917:Thrombin; D001241:Aspirin