Identification of mononuclear cells in cell suspension from cutaneous infiltrates freed by a suction blister method.
Mononuclear cells were liberated from superficial skin inflammations by a suction blister method which yielded a sufficient number of cells for histochemical and immunocytochemical analysis. The cell population consisted mainly of T cells which were identified as sheep red blood cell rosettes. Very few B cells were found as rosettes with immunobeads. Macrophages and eosinophils were found in varying numbers and they were identified histochemically. The method is suitable for studying nontumorous skin infiltrates.
D000328:Adult; D000505:Alopecia / Q000473:pathology; D000818:Animals; D001768:Blister; D002452:Cell Count; D003584:Cytological Techniques; D004485:Eczema / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D010267:Parapsoriasis / Q000473:pathology; D012397:Rosette Formation; D012867:Skin / Q000473:pathology*; D012871:Skin Diseases / Q000473:pathology*; D013396:Suction / Q000379:methods