[Synthesis of RNAs and proteins in hoplonemertinian oocytes with and without the effect of gonadostatin (GIH)].
Deprivation of gonadostatin (gonad-inhibiting hormone: GIH) performed during the quiescent period of the annual reproductive cycle in the hoplonemertean, Amphiporus lactifloreus, resulted in precocious sexual maturation. In female worms, the oometrical analysis showed that the hormone target, the ovary, responds to GIH deprivation by accelerated growth of a part of the oocyte population. The qualitative and quantitative histoautoradiographic examination of [3H]uridine and [3H]leucine incorporation enabled us to study RNAs and protein synthesis prerequisite and requisite for the oocyte growth. Early and continuous stimulation of protein synthesis in precociously growing oocytes was testified to by an increase in [3H]leucine incorporation into their ooplasm from day 6. On the other hand, later sequential stimulation of RNAs synthesis was observed, since a strong activation (induction?) of nucleoplasmic RNAs synthesis (tRNA and/or mRNA) from day 10 only took over from the slightly higher rDNA transcriptional activity seen in nucleolus from day 6 to day 9. By forcing the oocyte population of the ovary to retain a juvenile status, the inhibition of traduction into oocytes constitutes the earlier effect of gonadostatin. For bioassays by labelled precursor incorporation labelled aminoacids are the best candidates.
D000818:Animals; D001345:Autoradiography; D005260:Female; D007447:Invertebrate Hormones / Q000502:physiology*; D007448:Invertebrates / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D010827:Physiology; D010905:Pituitary Hormone Release Inhibiting Hormones / Q000502:physiology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism
D007447:Invertebrate Hormones; D010905:Pituitary Hormone Release Inhibiting Hormones; D012313:RNA; D007930:Leucine; D014529:Uridine
Synthèse de RNAs et de protéines dans les oocytes d'hoplonémertes soumis et soustraits à l'influence de la gonadostatine (GIH).