Suitability of control materials in the differential inhibition assay for human pancreatic and salivary amylase.
We investigated the behavior of 26 quality-control sera with the inhibitor method for differential amylase (EC 3.2.1.1) assay. We also studied the sensitivity to the wheat-derived inhibitor of pancreatic amylases from 10 different animals in comparison with human pancreatic and salivary amylase. The results indicate that only control materials containing human amylases can be measured accurately. The animal amylases (bovine, equine, porcine) used in many quality control sera are relatively insensitive to the inhibitor as compared with human pancreatic and salivary amylase.
D000681:Amylases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000592:standards; D000818:Animals; D002415:Cats; D002417:Cattle; D002645:Chickens; D004285:Dogs; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D010179:Pancreas / Q000201:enzymology*; D011786:Quality Control; D011817:Rabbits; D051381:Rats; D012463:Saliva / Q000201:enzymology*; D012756:Sheep; D013552:Swine
D000681:Amylases