title: A rapid method of concentrating proteins in small volumes with high recovery using Sephadex G-25.
abstract: A method for concentrating small (10 to 500 microliter) volumes of protein solutions by mixing in a microcentrifuge tube with measured volumes of dry Sephadex G-25 is described. The tube is centrifuged, and the protein solution concentrated up to fivefold passes through a small hole in the base of the tube and is collected. Recovery of protein is 90-95%.
mesh_terms: D001798:Blood Proteins / Q000302:isolation & purification; D003911:Dextrans; D005782:Gels; D008832:Microchemistry / Q000379:methods; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D001798:Blood Proteins; D003911:Dextrans; D005782:Gels; D011506:Proteins; C025614:sephadex