Absence of cell surface fixation of a monoclonal antibody detectable by conventional immunoassays does not exclude expression of and interaction with the corresponding antigenic determinant.
No specific binding of anti-HLA class I B.10.6 monoclonal antibody (mAb) could be demonstrated by cell surface radioimmunoassay and cytofluorographic studies at the surface of murine transformed L cells expressing HLA-A3 or Cw3 molecules. However, specific interaction of this antibody with these molecules at the surface of these transformed cells was indirectly established, since it inhibited specifically the binding to the same HLA class I molecules of other anti-HLA class I mAb. Therefore, the absence of detectable binding of mAb, in conventional immunoassays, does not exclude expression by these cells of the corresponding antigenic determinant.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D006680:HLA Antigens / Q000276:immunology; D015791:HLA-A3 Antigen; D015236:HLA-C Antigens; D006801:Humans; D051379:Mice; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D006680:HLA Antigens; D015791:HLA-A3 Antigen; D015236:HLA-C Antigens; C038653:HLA-C*03 antigen