title: The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
abstract: A rare cDNA coding for most of the alpha subunit of the Torpedo nicotinic acetylcholine receptor has been cloned into bacteria. The use of a mismatched oligonucleotide primer of reverse transcriptase facilitated the design of an efficient, specific probe for recombinant bacteria. DNA sequence analysis has enabled the elucidation of a large part of the polypeptide primary sequence which is discussed in relation to its acetylcholine binding activity and the location of receptor within the plasma membrane. When used as a radioactive probe, the cloned cDNA binds specifically to a single Torpedo mRNA species of about 2350 nucleotides in length but fails to show significant cross-hybridisation with alpha subunit mRNA extracted from cat muscle.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D004557:Electric Organ / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000235:genetics*; D011978:Receptors, Nicotinic / Q000235:genetics* / Q000378:metabolism; D014101:Torpedo
chemical_list: D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D011061:Poly A; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase