Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function of apo-cytochrome c-specific murine T cell clones.
A murine monoclonal antibody (SJL 2-4) specific for the antigen apo-cytochrome c was shown to inhibit both antigen-induced proliferation and lymphokine secretion by an apo-cytochrome c-specific BALB/c helper T cell clone. The inhibition was specific because additional apo-cytochrome c-specific T cell clones were not inhibited by the same monoclonal antibody. Time course studies of the inhibition indicated that the initial 8 hr of contact between T cell clones and antigen-presenting cells were critical for activation of the T cell clones. Inhibition of T cell functions by antigen-specific antibodies appeared to correlate with the antibody-antigen binding constant because a second monoclonal antibody (Cyt-1-59), with identical specificity but with a lower affinity constant for apo-cytochrome c, had very little inhibitory effect on the proliferation or lymphokine secretion of apo-cytochrome c-specific T cell clones.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001059:Apoproteins / Q000032:analysis / Q000276:immunology*; D001667:Binding, Competitive; D002454:Cell Differentiation; D002999:Clone Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D003574:Cytochrome c Group / Q000032:analysis / Q000276:immunology*; D045304:Cytochromes c; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000032:analysis; D007700:Kinetics; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D016215:Macrophage-Activating Factors; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001059:Apoproteins; D003574:Cytochrome c Group; D000939:Epitopes; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D045304:Cytochromes c