Macrophage-dependent activation of antigen-specific T cells requires antigen and a soluble monokine.
Antigen-induced T cell proliferation requires T cell interaction with antigen in the context of MHC I region-compatible accessory cells. The resulting activation and proliferation of T cells involves the production and utilization of several lymphokines or interleukins. This report describes experiments wherein these events could be separated into two phases, T cell activation and T cell proliferation. The first phase was achieved by stimulating antigen-specific T cell lines with antigen-pulsed ultraviolet light-irradiated accessory cells. T cell proliferation (second phase) could then be initiated by the addition of a soluble lymphokine with the characteristics of interleukin 1 (IL 1). These effects were only observed with homologous antigen and accessory cells syngeneic to the T cells at the I-A and E/C subregion of the MHC. This report has two applications in the study of lymphocyte-lymphokine interactions. First, T cell recognition of antigen and antigen-induced T cell proliferation can be examined as physically separate events. Secondly, this system may be used as a specific and sensitive means of measuring the effects of IL 1 on T cells.
D000818:Animals; D003208:Concanavalin A / Q000502:physiology; D000939:Epitopes; D007375:Interleukin-1 / Q000096:biosynthesis / Q000502:physiology; D007941:Leukemia P388 / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000528:radiation effects; D008222:Lymphokines; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D008805:Mice, Inbred A; D015846:Monokines; D010047:Ovalbumin / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D005719:gamma-Globulins / Q000276:immunology
D000939:Epitopes; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008222:Lymphokines; D015846:Monokines; D011506:Proteins; C021649:concanavalin A-induced helper factors; D005719:gamma-Globulins; D003208:Concanavalin A; D010047:Ovalbumin