title: Rapid screening of urine for significant bacteriuria by Gram stain, acridine orange stain, and the Autobac MTS system.
abstract: Urine specimens submitted for microbiologic examination were screened for evidence of bacteriuria by three rapid methods: Gram staining, acridine orange staining, and the Autobac MTS system. The screening results were compared with those obtained by semiquantitative colony counts on agar plates. In this comparative study 1055 urine specimens were examined, of which 146 (13.8%) had colony counts of greater than or equal to 1 X 10(5)/ml. All three urine screening methods detected this level of bacteriuria at a sensitivity of 98% and a specificity of 55.2% (acridine orange), 66.0% (Gram stain), and 83.2% (Autobac), respectively. Of the 1055 urine specimens examined, 185 (17.5%) had colony counts of greater than or equal to 1 X 10(4)/ml, at which level the sensitivity of the three methods was 93% and the specificity was 56.7% (acridine orange), 68.0% (Gram stain), and 86.0% (Autobac), respectively. For any level of sensitivity, the Autobac urine screen was shown to be more specific than either the Gram stain or the acridine orange method. The acridine orange stain was the least specific urine screen, especially at the upper limits of sensitivity.
mesh_terms: D000165:Acridine Orange; D001419:Bacteria / Q000302:isolation & purification*; D001437:Bacteriuria / Q000382:microbiology*; D005260:Female; D005840:Gentian Violet; D006801:Humans; D010619:Phenazines; D013194:Staining and Labeling
chemical_list: C035863:Gram's stain; D010619:Phenazines; D000165:Acridine Orange; D005840:Gentian Violet