H-2-determined kinetic differences for the induction of nucleoside-specific suppression.
The kinetic and the H-2 requirements for the induction of nucleoside-specific suppression were examined in several strains of mice; specifically, whether adenosine (A)-coupled spleen cells given intravenously suppress the primary response to adenosine-KLH. The adenosine system was chosen because C57Bl/6 mice were originally found to be resistant to immune suppression when challenged 5 days after treatment with adenosine-coupled spleen cells. (Raps et al. J. Immunol. 126, 1542, 1981.) It was determined (i) whether A-specific nonresponsiveness is inducible in strains other than C57Bl/6; (ii) whether changes in hapten density on the A-conjugated spleen cells could alter C57Bl/6s ability to become nonresponsive, and (iii) whether there are interstrain differences in the time required to induce A-specific suppressor T cells (Ts). The results show that there are H-2-associated differences in the time required to induce A-specific immune suppression. While A-spleen cells failed to suppress the A-specific response in C67Bl/10 (H-2b), they did induce unresponsiveness in B10.D2 (H-2d on C57Bl/10 background). A 2.5-fold increase in epitope density of adenosine on cells did not influence the kinetics of suppression. C67Bl/6 were resistant to suppression on Day 5, but like the CB6F1, susceptible to unresponsiveness 10 days after treatment. Nonresponsiveness was T-cell-mediated and transferable across IgH-V barriers. Suppression induced by Balb/c donor mice is transferable to Igh-incompatible CAL-20 mice. These results are discussed in the context of genetic restrictions which regulate suppressor T-cell interactions.
D000241:Adenosine / Q000276:immunology*; D000818:Animals; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007108:Immune Tolerance; D007116:Immunization, Passive; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000637:transplantation
D000939:Epitopes; D006183:H-2 Antigens; D007143:Immunoglobulin Heavy Chains; D006433:Hemocyanins; D000241:Adenosine