A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro.
In this paper we describe a method to select base pair substitution mutants constructed in vitro. The mutagenesis is performed by forcing mistakes during in vitro synthesis from a primer annealed to a single stranded DNA template. The selection is based on the fact that, following transformation, the progeny of the strand elongated in vitro and the template strand have different phenotypes. The method is general and applicable to any DNA segment; the type of base pair substitution and its position can be chosen at will. The combined efficiency of mutagenesis and selection allows for 85% frequency of mutants in all analyzed clones.
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification*; D008722:Methods; D009154:Mutation; D010641:Phenotype; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; D004247:DNA; D012194:RNA-Directed DNA Polymerase