Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains.
A novel H-2 gene in which the first external (N) domain of the H-2Ld antigen was replaced with that of the H-2Dd antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2Ld and H-2Dd antigens. It was found that serological determinants are distributed both in the N (Dd) and Cl (Ld) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and Cl domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.
D000818:Animals; D000937:Antigen-Antibody Reactions; D011232:Chemical Precipitation; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005796:Genes; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D009980:Influenza A virus / Q000276:immunology; D007739:L Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014170:Transformation, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
D000939:Epitopes; D006183:H-2 Antigens