[Fractionation of a sciatic nerve homogenate from normal, trembler and quaking mice on a continuous sucrose gradient].
Two fractions, characterized by biochemical criteria [quantity and density of particulate material, localization of myelin specific protein--Po, P1 and Pr--and of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) and of 5'-nucleotidase], were isolated from sciatic nerve homogenate of normal, Trembler and quaking, young and adult Mice, on a continuous sucrose gradient. The fraction, at 0.58-0.6 M, the richest in membrane particles contains the myelin. The fraction at 0.7-0.75 M should contain the plasma membrane of the Schwann cell which synthesizes myelin.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis; D015720:5'-Nucleotidase; D000375:Aging; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D051379:Mice; D008818:Mice, Neurologic Mutants; D004676:Myelin Basic Protein / Q000032:analysis; D018996:Myelin P2 Protein; D009708:Nucleotidases / Q000032:analysis; D012584:Sciatic Nerve / Q000201:enzymology / Q000254:growth & development / Q000648:ultrastructure*; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology
D004676:Myelin Basic Protein; D018996:Myelin P2 Protein; D009708:Nucleotidases; D015720:5'-Nucleotidase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases
Fractionnement d'un homogénat de nerf sciatique de souris normale, Trembler et quaking sur gradient continu de saccharose.