Carbodiimide and formalin as fixatives for the TP-levanol fast cyanine 5RN-method for staining myosin-like proteins in rat tissues.
Apart from the applicability of the tannic acid-phosphomolybdic acid-Levanol-Fast Cyanine 5RN method for the identification of muscle myosins in Carnoy-fixed tissues, this technique can also be applied on tissue sections fixed with formalin or carbodiimide. The use of carbodiimide as a fixative offers, in general, a superior staining of the tissues with safranine and Levanol. Using a slightly modified procedure, reduction of staining time in Levanol, moreover, allows a differentiation between cells containing different amounts of myosin. This is illustrated by means of a number of rat tissues.
D000818:Animals; D001391:Azo Compounds; D002234:Carbodiimides; D005404:Fixatives; D005557:Formaldehyde; D047348:Hydrolyzable Tannins; D008297:Male; D008982:Molybdenum; D009130:Muscle, Smooth / Q000032:analysis; D009132:Muscles / Q000032:analysis; D009218:Myosins / Q000032:analysis*; D010756:Phosphoric Acids; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods*
D001391:Azo Compounds; D002234:Carbodiimides; D005404:Fixatives; D047348:Hydrolyzable Tannins; D010756:Phosphoric Acids; D005557:Formaldehyde; C015967:Levanol Fast Cyanine 5RN; D008982:Molybdenum; D009218:Myosins; C003125:phosphomolybdic acid