Modified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms.
We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-rich M components of immunoglobulins M and A in serum and in cerebrospinal fluid from patients with plasma cell dyscrasia are readily detected. The technique is two- to threefold more sensitive than Ponceau S. Glycoproteins in serum and body fluids can be determined as a routine analytical procedure.
D004587:Electrophoresis, Agar Gel / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D010265:Paraproteinemias / Q000097:blood / Q000134:cerebrospinal fluid; D010503:Periodic Acid-Schiff Reaction; D013194:Staining and Labeling
D006023:Glycoproteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M