title: Chick oviduct progesterone receptor: structure, immunology, function.
abstract: Analysis of the purified chick oviduct progesterone receptor using biochemical and immunological approaches indicates that while the 'activated' receptor ('4S') is a mixture of two progestin-binding polypeptides, 'A' (Mr approximately 79 kDa) and 'B' (Mr approximately 110 kDa), the non-activated receptor ('8S') is a population of complexes containing a hormone-binding polypeptide (A or B, but probably not both) bound to a non-hormone-binding protein (Mr approximately 90 kDa). Two molecules of the 90 kDa protein appear to be present in each '8S' receptor molecule. The 90 kDa protein is also associated with the non-activated forms of receptors of other steroid hormones in the chick. Molybdate stabilizes the non-activated receptors, probably by forming weak coordination bonds with radicals provided by the subunits of the '8S' structure. Activation implies separation of the subunits, without a change in their primary structure, and does not require intervention of any protein other than those present in the '8S' receptor form. The presence of ligand at the binding site accelerates the activation process but, in vitro, is not necessary for it to occur. Unlike the non-activated form, activated receptors bind to the cell nuclei. However, histological studies with anti-progesterone receptor antibodies indicate that in the non-hormone-exposed tissue the (non-activated) receptors could be localized in the nuclei.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004355:Drug Stability; D000939:Epitopes / Q000276:immunology; D005260:Female; D007700:Kinetics; D046911:Macromolecular Substances; D008982:Molybdenum / Q000494:pharmacology; D010057:Oviducts / Q000032:analysis*; D010446:Peptide Fragments / Q000378:metabolism; D010447:Peptide Hydrolases; D011374:Progesterone / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011980:Receptors, Progesterone / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011980:Receptors, Progesterone; D011374:Progesterone; D008982:Molybdenum; D004247:DNA; D010447:Peptide Hydrolases