In vivo assembly of regularly spaced nucleosomes on mouse beta maj-globin DNA cloned in an SV40 recombinant.
Short-term transformation of HeLa cells with an SV40 recombinant carrying a 7.0-Kb mouse genomic globin DNA was studied. It was found that 48 h after transfection the donor DNA was present in the cell nucleus at high copy episomal numbers with the globin gene in regularly spaced nucleosomal form and transcribed into 9S poly A+ RNA. These mini-chromosomes can be isolated in sufficient quantity to allow further biochemical and electron microscopic studies.
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D005914:Globins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009707:Nucleosomes / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*
D009707:Nucleosomes; D012313:RNA; D005914:Globins; D004247:DNA