Description of monoclonal antibody defining an HLA allotypic determinant that includes specificities within the B5 cross-reacting group.
The selection and characterization of a cloned murine monoclonal antibody (4D12) that defines an HLA allotypic determinant is described. Antibody 4D12 immunoprecipitates HLA heavy and light chains. From studies on 4D12 reactivity with a large panel of well-characterized target cells, antibody 4D12 defines a public HLA determinant that includes specificities within but not identical to the B5 cross-reacting group. Antibody 4D12 should be useful in the study of HLA-B5--related supratypic antigens.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D015235:HLA-B Antigens; D006650:Histocompatibility Testing; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015235:HLA-B Antigens; C035270:HLA-B5 antigen; D007126:Immunoglobulin Allotypes