Optimal detection of oligoclonal bands in CSF by silver stain.
We applied a sensitive silver nitrate stain to SDS-polyacrylamide gels to detect oligoclonal bands in unconcentrated CSF. Silver staining took less than 3 hours and gave easily readable bands in 93% of MS patients. In contrast, standard Coomassie Blue stain was positive in only 53%. Maximum resolution and numbers of bands resulted when silver staining was used on 10 to 20 microliters of CSF. Silver staining of SDS-polyacrylamide gels permits reliable oligoclonal band detection on small volumes of unconcentrated CSF.
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D012835:Silver Nitrate; D013194:Staining and Labeling
D007074:Immunoglobulin G; D007136:Immunoglobulins; D012835:Silver Nitrate