title: Effect of alpha-amanitin and actinomycin D on RNA synthesis in the protoplasts of Aspergillus nidulans.
abstract: RNA synthesis was studied in protoplasts from Aspergillus nidulans, which remained metabolically active for several days under the appropriate conditions. It is concluded, that at certain actinomycin D concentrations the ribosomal RNA synthesis can be selectively inhibited. However, alpha-amanitin at concentrations up to 400 micrograms/ml neither influenced the total nor the RNA polymerase II directed RNA synthesis. It was possible to discriminate between the RNA polymerase I or II directed RNA synthesis due to the presence of poly(A) tails attached to at least part of the RNA, presumably messenger RNAs, or their precursors.
mesh_terms: D000546:Amanitins / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000187:drug effects* / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D011061:Poly A / Q000096:biosynthesis; D011523:Protoplasts / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012319:RNA Polymerase II / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis*; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000096:biosynthesis
chemical_list: D000546:Amanitins; D012331:RNA, Fungal; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D003609:Dactinomycin; D011061:Poly A; D012313:RNA; D012319:RNA Polymerase II