title: Monoclonal antibody defining a Burkitt's lymphoma-associated antigen detects carbohydrate on neutral glycolipid.
abstract: Monoclonal antibody 38.13 detects an epitope presented on Burkitt's lymphoma cells that has not been detected on other lymphoid tissues or tumors. The biochemical nature of this Burkitt's lymphoma-associated antigen was investigated by staining inhibition assays. Indirect immunofluorescence and fluorescence-activated cell sorter analysis show that antibody 38.13 reacts with a protease-resistant structure present among the hydrophobic components extracted with chloroform-methanol from the Ramos Burkitt's cell line. These components were further separated by ion-exchange chromatography and activity resides in the unbound (neutral) fraction. The staining antibody is specifically absorbed when preincubated with D-galactose, and to a lesser extent with N-acetyl-galactosamine. Therefore, 38.13 appears to recognize a determinant of carbohydrate nature, which is carried on a neutral glycolipid.
mesh_terms: D000042:Absorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D001666:Binding Sites, Antibody; D002051:Burkitt Lymphoma / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D006017:Glycolipids / Q000276:immunology; D010447:Peptide Hydrolases / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002241:Carbohydrates; D000939:Epitopes; D006017:Glycolipids; D010447:Peptide Hydrolases