Developmental changes in serotonin and 5-hydroxyindoleacetic acid concentrations and opiate receptor binding in rat spinal cord following neonatal 5,7-dihydroxytryptamine treatment.
Normal levels of serotonin and 5-hydroxyindoleacetic (5-HIAA) were measured in the developing rat spinal cord and brain stem using HPLC. Serotonin, 5-HIAA and 3H-naloxone binding were monitored following neonatal 5,7-dihydroxytryptamine (5,7-DHT) treatment. Serotonin in the spinal cord develops biphasically with peaks at gestation day 21 (E21) and postnatal day 8 (P8) while 5-HIAA peaks at P3 and between P8 and P11. In the brain stem, serotonin gradually rises from E15 to adult levels which are reached by P32 while 5-HIAA rises sharply to P15 and thereafter decreases to adult levels by P32. 5,7-DHT treatment neonatally causes greater than 50% reduction of serotonin and 5-HIAA in the spinal cord through P32. Opiate receptor binding in the spinal cord is reduced acutely by about 22% and remains slightly depressed into adulthood.
D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D004102:Dihydroxytryptamines / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D008526:Medulla Oblongata / Q000254:growth & development; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism*; D012701:Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism
D004102:Dihydroxytryptamines; D011957:Receptors, Opioid; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid