title: 5 S RNA-protein complex is involved in ribosomal subunit association.
abstract: The immobilized tRNA-50 S ribosomal subunit protein (TP50) complex binds the smaller ribosomal subunit. We constructed tRNA . TP50 . 5 S [32P] RNA and tRNA . TP50 . t [32P] RNA complexes and investigated the accessibility of the 32P-labelled tRNAs to ribonuclease T1. It was found that in this complex both 5 S RNA and tRNA are attacked by T1 RNase. In sharp contrast, the addition of 30 S subunit protects 5 S RNA as well as tRNA from degradation. We suggest that 5 S RNA-TP50 complex is exposed to the ribosomal interface and is involved in subunit interaction.
mesh_terms: D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000032:analysis*; D046911:Macromolecular Substances; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D006163:Ribonuclease T1 / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D012329:RNA, Bacterial; D012269:Ribosomal Proteins; D012343:RNA, Transfer; D006163:Ribonuclease T1