Metabolic studies on vaccinia-virus-infected oligodendrocytes in brain cell cultures.
Twelve-day-old cultures of dissociated newborn mouse brain were infected with neurotropic vaccinia virus strain WR. Using the indirect immuno-fluorescence staining technique the total destruction of galactocerebroside (GL) or myelin basic protein (MBP)-positive oligodendrocytes could be detected after 72 h of infection. The activity of the oligodendrocyte-specific enzymes, cerebroside sulfotransferase (CST) and 2'3'-cyclic nucleotide 3'-phosphohydrolase (CNP), was 27% and 17% respectively of the activity in noninfected controls. This reduction was not a result of viral-induced inhibition of host protein synthesis. In cultures treated with puromycin GC- and MBP- positive oligodendrocytes were detectable at a time at which no CST or CNP activity could detected.
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D004660:Encephalitis / Q000378:metabolism*; D005699:Galactosylceramides / Q000378:metabolism; D051379:Mice; D004676:Myelin Basic Protein / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D011691:Puromycin / Q000494:pharmacology; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism; D014615:Vaccinia / Q000378:metabolism*; D014616:Vaccinia virus / Q000378:metabolism*
D005699:Galactosylceramides; D004676:Myelin Basic Protein; D011691:Puromycin; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571338:Cnp protein, mouse