Limitations to the usefulness of N-succinimidyl propionate for the study of retrograde axonal transport.
Retrograde axonal transport of radiolabelled proteins was studied in rat sciatic nerve, after direct application of [3H]N-succinimidyl propionate. Waves of radiolabelled proteins were observed but only two proteins were predominantly labelled, one of molecular weight 68 kilodaltons (K) and the other of 19K. There was no evidence to confirm the waves as representing retrograde axonal transport of identifiable proteins, and the tendency of the covalent label to bind selectively in vivo to a small number of prominent proteins limits its usefulness for the detection of retrogradely transported proteins in general.
D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D005260:Female; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000000981:diagnostic imaging*
D009419:Nerve Tissue Proteins; D011422:Propionates; C021749:N-succinimidyl propionate