Reconstitution of 50 S ribosomal subunits from Bacillus stearothermophilus with 5 S RNA from spinach chloroplasts and low-Mr RNA from mitochondria of Locusta migratoria and bovine liver.
Reconstitution experiments with 50 S ribosomal subunits from Bacillus stearothermophilus demonstrate that spinach chloroplast 5 S rRNA can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5 S rRNA. In contrast, mitochondria from Locusta migratoria or bovine liver do not appear to contain discrete, low-Mr RNAs, which can replace 5 S rRNA in the functional reconstitution of B. stearothermophilus ribosomes.
D000818:Animals; D005075:Biological Evolution; D002417:Cattle; D002736:Chloroplasts / Q000032:analysis*; D001411:Geobacillus stearothermophilus / Q000032:analysis*; D006110:Grasshoppers / Q000032:analysis; D008928:Mitochondria / Q000032:analysis*; D008930:Mitochondria, Liver / Q000032:analysis; D010452:Peptide Biosynthesis; D010455:Peptides; D010944:Plants; D012329:RNA, Bacterial / Q000502:physiology; D012335:RNA, Ribosomal / Q000502:physiology*; D012270:Ribosomes / Q000502:physiology*; D013045:Species Specificity
D010455:Peptides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; C012001:polyphenylalanine