Regulation of immune responses in SJL and F1 hybrid mice by gamma-irradiated syngeneic lymphoma cells.
Syngeneic mixed lymphocyte-stimulating la+ lymphomas of SJL mice [reticulum cell sarcoma(s) (RCS)] were found to modulate immune responses in vivo. Simultaneous injection of 2 X 10(7) gamma-irradiated or glutaraldehyde-fixed RCS cells with the antigen sheep red blood cells (SRBC) or 2,4,6-trinitrophenol (TNP)-Ficoll markedly suppressed the subsequent plaque-forming cell response in the spleen. The suppression of the anti-SRBC response was prevented by pretreatment of the mice with cyclophosphamide, whereas the suppression of the anti-TNP-Ficoll response was not affected. RCS injection induced high interferon serum titers within 24 hours after injection, which were not prevented by pretreatment with cyclophosphamide. Injection of gamma-irradiated RCS cells (gamma-RCS) or RCS cell extract 2 days prior to antigen enhanced the anti-SRBC but markedly suppressed the anti- TNP-Ficoll response. Injection of RCS both on day -2 and day 0 enhanced the anti-SRBC response. SJL mice 8-9 months of age showed much less or no suppression when gamma-RCS cells were injected on day 0. Certain F1 hybrids of SJL also showed the gamma-RCS-induced suppression of the anti-SRBC response. Suppression was seen in SJL X BALB.B but not in SJL X BALB/c mice and in SJL X A.TH but not in SJL X A.TL mice, suggesting an I-region effect. F1 hybrids of SJL by B10 background mice showed no significant suppression. Enhancement of the anti-SRBC response by prior injection of gamma-RCS was seen in all F1 hybrid mice examined.
D000375:Aging; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects*; D002588:Cesium Radioisotopes; D003520:Cyclophosphamide / Q000494:pharmacology; D005720:Gamma Rays; D005976:Glutaral / Q000494:pharmacology; D006824:Hybridization, Genetic; D007372:Interferons / Q000008:administration & dosage; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology*; D051379:Mice; D009368:Neoplasm Transplantation; D014181:Transplantation Immunology
D000941:Antigens; D002588:Cesium Radioisotopes; D003520:Cyclophosphamide; D007372:Interferons; D005976:Glutaral