The effects of methylprednisolone acetate on macromolecular synthesis and glucose oxidation in epithelial cells of the ocular surface.
Epithelial cells that re-epithelialized corneas after the normal corneal epithelium was removed limbus to limbus by scraping were found to be metabolically responsive to methylprednisolone acetate. Seventy-two hours after scraping, the re-epithelializing cells from steroid-treated animals were found to have a 26% slower rate of DNA synthesis, a 30% slower rate of glucose oxidation via glycolysis, and a 79% greater rate of RNA synthesis with no apparent change in gross protein synthesis as compared with vehicle-treated controls. The DNA content of the epithelial layer isolated from steroid-treated corneas was reduced by 31% as compared with that of vehicle-treated controls.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D003315:Cornea / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis*; D004848:Epithelium / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects*; D008775:Methylprednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077555:Methylprednisolone Acetate; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D011817:Rabbits
D000596:Amino Acids; D000077555:Methylprednisolone Acetate; D012313:RNA; D004247:DNA; D008775:Methylprednisolone