Phospholipase A activation following extracorporeal circulation and its blockade by procaine.
The phospholipase A activity of human plasma was investigated in the course of operations with the aid of extracorporeal circulation. After an initial increase following heparin application, there is a continuous rise up to the end of perfusion and a decrease to normal values 30 minutes after the end of perfusion and the application of protamine. Procaine is suitable for the inhibition of the increased phospholipase A activity. In accordance with in vitro determinations of an effective dose of 1 mmol/ml, corresponding concentrations were attained with an infusion rate of 1.5-2 mg/kg/min. In a second series of investigations, a significantly smaller phospholipase A activation in the course of perfusion was detectable through the addition of procaine.
D001026:Coronary Artery Bypass; D004789:Enzyme Activation / Q000187:drug effects; D005112:Extracorporeal Circulation / Q000009:adverse effects*; D006350:Heart Valve Prosthesis; D006493:Heparin / Q000009:adverse effects; D006494:Heparin Antagonists / Q000627:therapeutic use; D006801:Humans; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D011343:Procaine / Q000008:administration & dosage / Q000627:therapeutic use*; D011479:Protamines / Q000494:pharmacology
D006494:Heparin Antagonists; D011479:Protamines; D011343:Procaine; D006493:Heparin; D010740:Phospholipases; D010741:Phospholipases A