Role of accessory cells in the induction of a secondary cytotoxic response to Moloney murine sarcoma virus-induced tumors.
The role of Ia-positive accessory cells in the generation of a secondary cytotoxic response to tumor-associated antigens induced by Moloney murine sarcoma virus (M-MSV) was evaluated. Spleen cells from M-MSV-immune A.TL mice, depleted of accessory cells by anti-Iak serum plus C treatment and stimulated in secondary mixed leukocyte tumor cell culture (MLTC) with syngeneic Ia-negative A6ATL Moloney leukemic cells, failed to generate virus-specific cytotoxic T lymphocytes (CTL). CTL generation in Ia-depleted MLTC may be reconstituted by the addition of nonimmune Ia-positive spleen or peritoneal cells obtained not only from syngeneic A.TL but also from I-incompatible A.TH mice. This lack of restriction observed in accessory cell function is explained in terms of a nonspecific mechanism of CTL triggering mediated by soluble factors. In fact, IL 2 as well as supernatants obtained from I region-incompatible cultures consisting of M-MSV-immune, Ia-depleted A.TL spleen cells and A.TH Ia-positive cells, reconstituted secondary virus-specific CTL generation.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007111:Immunity, Cellular; D051379:Mice; D008805:Mice, Inbred A; D008979:Moloney murine leukemia virus / Q000276:immunology; D012513:Sarcoma, Experimental / Q000209:etiology / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D000949:Histocompatibility Antigens Class II