Monoclonal antibodies against different antigenic determinants of a protein molecule (human serum albumin) as detected after labeling the antigen with two different markers.
By using FITC- and 125I-labeled human serum albumin (HSA) the reactivity of anti-HSA hybridoma antibodies was compared in solid-phase immunofluorescence assay (SIFA) and radioimmunoassay (SRIA). A considerable number of hybridoma antibodies had been detected which are positive in SIFA but not in SRIA. Since this cannot be explained by a difference in the sensitivity of the assays it seems likely that these monoclonal antibodies detect antigenic determinants which contain tyrosine as an essential part.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000941:Antigens / Q000032:analysis; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007457:Iodine Radioisotopes; D051379:Mice; D011863:Radioimmunoassay; D012709:Serum Albumin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D007457:Iodine Radioisotopes; D012709:Serum Albumin