Monoclonal antibodies reveal three types of idiotypic determinants on MOPC 173: H-L-specific private and public idiotopes, and Vh-specific public idiotope(s).
Monoclonal anti-idiotypic antibodies against the IgG2a, K myeloma protein MOPC 173 were raised with A/J- and CBA-immune B-cells. The specificity of the antibodies was studied by hemagglutination and inhibition of a radioimmunoassay (RIA). A series of myeloma proteins, their H- and L-chains, free or reassociated in various combinations, were used as inhibitors. The RIA patterns allow recognition of three types of idiotypic determinants: private conformational idiotope(s), a public conformational idiotope, and public VH-determinant(s). Strain distribution of the public determinant was studied. A tentative correlation between amino acid positions and Id determinants is discussed.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007525:Isoelectric Focusing; D051379:Mice; D008815:Mice, Inbred Strains; D009194:Myeloma Proteins / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins; C035772:myeloma protein MOPC 173