Detection of intranuclear clusters of Sm antigens with monoclonal anti-Sm antibodies by immunoelectron microscopy.
It has been shown that small nuclear RNA (snRNA) species U1, U2, U4, U5, and U6 are found in the nucleus in the form of small nuclear ribonucleoprotein particles (snRNPs), and that anti-Sm antibodies react with snRNP polypeptides, which are associated with all five snRNAs. We report here a novel intranuclear complex, denoted "Sm cluster," detected by immunostaining with monoclonal anti-Sm antibodies in HeLa cells.
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D001324:Autoantigens; D002467:Cell Nucleus / Q000276:immunology* / Q000648:ultrastructure; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D012313:RNA / Q000032:analysis*; D012342:RNA, Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins
D000911:Antibodies, Monoclonal; D000941:Antigens; D001324:Autoantigens; D012342:RNA, Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D012313:RNA