Purification of a glycoprotein bearing a tumor transplantation antigen specific for Friend, Moloney, and Rauscher MuLV-induced tumors.
A 175,000 dalton glycoprotein bearing a tumor-associated transplantation antigen (TATA) has been isolated from a Rauscher-MuLV-induced leukemia (RBL-5). The glycoprotein has the properties expected for a Friend, Moloney, or Rauscher-MuLV-induced TATA: 1) It is synthesized by RBL-5 cells and is expressed on the cell surface. 2) When inoculated into mice, it provides complete protection against challenge with RBL-5 cells at doses of 1 microgram or less. 3) The same protein, isolated from a Moloney-MuLV-induced leukemia, also protects mice against challenge with RBL-5. A similar glycoprotein is expressed on other tumor cells and on normal cells that do not express a TATA cross-reactive with RBL-5. Therefore, the immunogenicity of this molecule cannot be principally accounted for by either its unique or elevated expression on RBL-5 cells. A model is presented that accounts for these properties of the RBL-5 TATA. In addition, two other features of the RBL-5 TATA are discussed; an apparent abrogation of immunity seen only with certain doses of antigen, and a loss of the inherent immunogenicity of gp175 during purification.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D000939:Epitopes; D005622:Friend murine leukemia virus / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D006183:H-2 Antigens / Q000276:immunology; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D007942:Leukemia, Experimental / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008979:Moloney murine leukemia virus / Q000276:immunology; D011817:Rabbits; D011925:Rauscher Virus / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*
D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D000939:Epitopes; D006023:Glycoproteins; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D014764:Viral Proteins; C029237:tumor-associated transplantation antigen