Sensitivity of a neoplastic epithelial duct cell line from a human submandibular salivary gland to human leukocyte interferon as assessed by an in vitro semi-solid agar technique.
A neoplastic epithelial duct cell line from a human submandibular salivary gland, HSG, was assayed by an in vitro semi-solid agar technique for sensitivity to human leukocyte interferon (HuIFN-alpha). The clone which showed the most stable growth and the greatest ability of colony formation in the agar medium, was isolated from the colonies of HSG cells and used in the present study. This clone was designated as HSG-1. As a consequence, cultivation of HSG-1 cells in the agar medium containing HuIFN-alpha of 1000 units/ml resulted in the inhibition of 47.6% of the colony-forming ability of HSG-1 cells, as compared with the untreated control. Addition of increasing concentrations of HuIFN-alpha (between 100 units/ml and 1000 units/ml) in the agar medium caused a linear reduction in the number of the colonies, ranging from 5.2% to 47.6%. Moreover, addition of HuIFN-alpha showed an inhibitory effect on the multiplication of HSG-1 cells in the monolayer culture. These observations strongly suggest that HuIFN-alpha has antineoplastic and antiproliferative effects on HSG-1 cells.
D000362:Agar; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D007372:Interferons / Q000627:therapeutic use*; D007962:Leukocytes / Q000502:physiology*; D009522:Newcastle disease virus; D012468:Salivary Gland Neoplasms / Q000628:therapy*; D013365:Submandibular Gland Neoplasms / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D013997:Time Factors
D003470:Culture Media; D000362:Agar; D007372:Interferons