Single doses of acrylamide reduce retrograde transport velocity.
Single doses of acrylamide (0-1.3 mmol/kg) produced a dose-dependent decrease in the transport of 125I-tetanus toxin to the perikarya of sensory neurons in dorsal root ganglia and motor neurons in ventral spinal cord. Acrylamide was a more potent inhibitor of retrograde transport in sensory axons than in motor axons. Substantially greater doses of N,N'-methylene-bis-acrylamide, a reportedly non-neurotoxic analog of acrylamide, were required to alter the axonal transport of 125I-tetanus toxin. Velocity of retrograde transport was assessed by determining the position of the leading edge of transported 125I-tetanus toxin at times following single doses of acrylamide. Acrylamide reduced the velocity of 125I-tetanus toxin transport in a dose-dependent manner by up to 75%. No change in neuronal uptake of 125I-tetanus toxin was detected. It is concluded that single doses of acrylamide produce profound alterations in retrograde transport which precede the appearance of structural changes in affected nerve fibers.
D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D005727:Ganglia, Spinal / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013744:Tetanus Toxin / Q000378:metabolism
D000178:Acrylamides; D007457:Iodine Radioisotopes; D013744:Tetanus Toxin; D020106:Acrylamide; C021221:N,N'-methylenebisacrylamide