title: Lipid-bound sugars in malignant human breast tumors. Partial characterization of mannosyl and glucosyl transferase activities.
abstract: Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-[14C]-Man and UDP-[14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phosphate mannose and dolichyl phosphate glucose whereas lipid-linked oligosaccharides behaved as polyprenyl diphosphate derivatives. The optimum conditions for mannosyl- and glucosyl-transfer reactions and the effect of dolichyl phosphate, detergent and EDTA on incubation mixture were described.
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology*; D002250:Carbon Radioisotopes; D005260:Female; D005964:Glucosyltransferases / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008364:Mannosyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000096:biosynthesis; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D006023:Glycoproteins; D009702:Nucleoside Diphosphate Sugars; D011104:Polyisoprenyl Phosphate Oligosaccharides; D014539:Uridine Diphosphate Sugars; D006155:Guanosine Diphosphate Mannose; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D014532:Uridine Diphosphate Glucose