Antibody combining sites can be mimicked synthetically. Surface-simulation synthesis of the phosphorylcholine-combining site of myeloma protein M-603.
By applying the concept of 'surface-simulation' synthesis to the combining site of the myeloma protein M-603 we were able to mimic synthetically its phosphorylcholine-binding characteristics. The synthetic surface-simulation peptide was found to bind to phosphorylcholine, whereas a control peptide that had the same amino acid composition but a different sequence showed little or no binding activity. The specificity of the binding was further confirmed by inhibition studies in which the surface-simulation peptide, but not the control peptide, inhibited the binding of 125I-labelled surface-simulation peptide to phosphorylcholine. Furthermore, the surface-simulation peptide was found to completely inhibit the binding of the native myeloma protein, M-603, to phosphorylcholine. The control peptide was unable to inhibit this binding. These findings suggest that surface-simulation synthesis can be effectively employed to mimic synthetically antibody combining sites, and may in the future be a valuable tool with which to manipulate the immune response to clinically important antigens.
D000818:Animals; D000936:Antigen-Antibody Complex; D001665:Binding Sites; D000939:Epitopes; D007070:Immunoglobulin A; D007136:Immunoglobulins / Q000276:immunology*; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D009194:Myeloma Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000032:analysis; D010767:Phosphorylcholine
D000936:Antigen-Antibody Complex; D000939:Epitopes; D007070:Immunoglobulin A; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D010446:Peptide Fragments; C017551:myeloma immunoglobulins; D010767:Phosphorylcholine