Surface carbohydrate epitopes of Thy-1.1 and Thy-1.2 thymocytes are distinguished by a monoclonal antibody with a specificity common to peanut agglutinin.
NIMF4-31.7 is a monoclonal IgM antibody with affinity for terminal galactose residues of membrane glycoproteins, Sepharose and agarose. It is cytotoxic for a subpopulation of murine thymocytes and peripheral lymphocytes, corresponding to the peanut agglutinin-positive population, but reacts with a majority of cells after neuraminidase treatment. It has revealed a genetic linkage between the Thy-1 locus and quantitative expression of carbohydrate determinants on the thymocyte membrane.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D007152:Immunologic Capping; D037102:Lectins / Q000276:immunology*; D051379:Mice; D009844:Oligosaccharides / Q000037:antagonists & inhibitors; D019887:Peanut Agglutinin; D012685:Sepharose / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D037102:Lectins; D009844:Oligosaccharides; D019887:Peanut Agglutinin; D012685:Sepharose