Immunocytochemical localization of the glycoprotein hormone alpha subunit in the median eminence of the mouse hypothalamus.
Immunoperoxidase staining was performed to localize glycoprotein hormone alpha subunit in mouse brain. Staining was found only in the median eminence with the use of ovine LH-alpha antiserum and was diffuse, not localized to fibers but to pericapillary areas. Absorption of the antiserum with bovine TSH-alpha subunit completely abolished the staining. These findings, in conjunction with our earlier studies in which alpha subunit messenger RNA was not detected in mouse brain, suggest that alpha subunit reaches the hypothalamus by retrograde diffusion from the pituitary.
D000818:Animals; D015292:Glycoprotein Hormones, alpha Subunit; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008473:Median Eminence / Q000032:analysis*; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis*; D010908:Pituitary Hormones, Anterior / Q000032:analysis*
D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior