Comparison of different methods for large-volume concentration of a type D retrovirus (PMFV).
Different methods of large-scale concentration of a type D retrovirus (PMFV) were compared, including continuous-flow centrifugation, ultrafiltration, and polyethylene glycol precipitation. Best results were obtained by a combination of continuous-flow centrifugation and subsequent purification by velocity gradient and isopycnic gradient centrifugation.
D002460:Cell Line; D002498:Centrifugation / Q000379:methods; D006801:Humans; D008854:Microscopy, Electron; D011092:Polyethylene Glycols; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure; D012208:Rhabdomyosarcoma; D014462:Ultrafiltration / Q000379:methods
D011092:Polyethylene Glycols; D012194:RNA-Directed DNA Polymerase