Granular and globular acid alpha-naphthyl-acetate-esterase staining pattern of resting and mitogen-activated murine lymphocytes.
Available methods for the identification of T-lymphocytes in tissue sections and in single cell suspensions are often tedious and require a lot of manipulation. Here we confirm previous findings that nonspecific alpha-naphthyl-acetate esterase (ANAE) is a reliable method for the identification of resting thymus-derived lymphocytes but not for concanavalin-A-stimulated lymphocytes, as a small proportion of lipopolysaccharide-stimulated lymphoblasts exhibit ANAE activity as well. We have also shown that there is a relationship between the globular staining pattern and the degree of cell activation. Finally, we noted that prolonged incubation of lymphocytes at 37 degrees C reduces the number of ANAE-positive cells.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D002265:Carboxylic Ester Hydrolases / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009283:Naphthol AS D Esterase / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000187:drug effects; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects
D008070:Lipopolysaccharides; D003208:Concanavalin A; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases