Immunochemical studies on the O-specific side chains of the heterogeneous lipopolysaccharide from Proteus mirabilis O27.
Chemically and serologically identical O-specific fractions were isolated from two different lipopolysaccharide (LPS) preparations of Proteus mirabilis O27. The release of a labile constituent in mild hydrolysis deprived those fractions of their serological activity. The compound was identified as N-acetyl-D-glucosamine and its terminal position in the molecule was established by methylation analysis. The non-carbohydrate constituent of the specific fractions: lysine and alanine are linked via their amino group. Terminal residues of N-acetylglucosamine, have to be considered as immunodeterminants of Proteus mirabilis O27. The role of other carbohydrate and non-carbohydrate constituents for the serological specificity of P. mirabilis O27 is discussed.
D000939:Epitopes; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011513:Proteus mirabilis / Q000276:immunology*
D000939:Epitopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial