title: Complexity of polysomal polyadenylated RNAs in Vicia faba meristematic root cells.
abstract: As a first step in quantifying gene expression during differentiation of the Vicia faba root cells we have analysed the mRNA population from meristematic cells. Using cDNA X mRNA hybridizations we have shown that this population can be divided into three abundance classes (abundant, intermediate and rate) representing 37%, 34% and 29% of the mRNAs and containing 26, 610 and 11700 sequences respectively. The total base-sequence complexity of the mRNA population, as determined by cDNA X mRNA hybridization, was found to be 1.6 x 10(7) nucleotides. This estimate was confirmed by the determination of the amount of single-copy genomic DNA hybridizing to the mRNA. Expressed as the number of structural gene transcripts, this complexity corresponds to about 13000 mRNA sequences.
mesh_terms: D004247:DNA / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010946:Plants, Medicinal; D011061:Poly A / Q000302:isolation & purification*; D011132:Polyribosomes / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA; D004247:DNA