title: Determination of glycosylated adult and foetal haemoglobins by affinity chromatography.
abstract: Estimation of adult glycosylated haemoglobin by affinity chromatography was found to be quick and less dependent on ionic strength, pH and temperature than ion-exchange chromatography. Results obtained by both procedures correlated strongly (r = 0.96) but the range for normal subjects was smaller with the affinity assay. The affinity method correlated equally well with the colorimetric assay (r = 0.95). However, the method did not measure all the glycosylated forms, and only half of the glycosylated species isolated by ion-exchange chromatography was bound to the affinity resin. It also showed that the amount of glycosylated haemoglobin in cord blood is less than in adult blood.
mesh_terms: D000375:Aging; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange / Q000379:methods; D003124:Colorimetry / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D005319:Fetal Hemoglobin / Q000031:analogs & derivatives* / Q000032:analysis; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D011247:Pregnancy
chemical_list: D005319:Fetal Hemoglobin; D006442:Glycated Hemoglobin; C029736:glycated fetal hemoglobin