Rapid, simultaneous quantification of morphine, codeine, and hydromorphone by GC/MS.
Morphine, codeine, and hydromorphone were extracted from blood or serum using a one step extraction. The extract was derivatized and the trifluoroacetyl opiates were quantified by GC/MS selected ion monitoring (SIM) using nalorphine as the internal standard. Calibration curves were linear and sensitivity as low as .02 mg/L for morphine and codeine, and .08 mg/L for hydromorphone, was achieved. Urine or tissue homogenates could be processed similarly after acid hydrolysis.
D001322:Autoanalysis; D003061:Codeine / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D004091:Hydromorphone / Q000032:analysis*; D009020:Morphine / Q000032:analysis*
D009020:Morphine; D004091:Hydromorphone; D003061:Codeine