title: Antigenic determinant of the Lancefield group H antigen of Streptococcus sanguis.
abstract: Previous studies indicated that the teichoic acid isolated from strains of Streptococcus sanguis was group specific and defined the Lancefield group H streptococci. To determine the specific antigenic determinants, the antigen was extracted from a group H streptococcus (ATCC 903) by the phenol-water method and purified by column chromatography. The isolated antigen had a glycerol/phosphate/glucose molar ratio of 1:0.9:0.3; the lipid concentration was 7.6% of its dry weight. No nucleic acids were detected, and amino acids constituted approximately 2% of the dry weight. The minimum concentration of antigen required to sensitize erythrocytes for hemagglutination with a 1:1,000 dilution of either group H antiserum or antiteichoic acid serum was 0.02 microgram/ml. Hemagglutination inhibition studies suggested that the major antigenic determinant consisted of an alpha-glucose linked to the glycerol phosphate backbone.
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000939:Epitopes; D005947:Glucose / Q000276:immunology; D006384:Hemagglutination; D013298:Streptococcus sanguis / Q000145:classification / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D013682:Teichoic Acids; D005947:Glucose