Production of interferon and other lymphokines during murine tumour growth. III. Characterization of microbial growth inhibitory activity in cell-free supernatants of rat Zajdela ascites hepatoma.
A microbial growth inhibitory factor (MGIF) detected in the cell-free fluid of Zajdela ascites hepatoma of the rat was characterized by means of gel filtration, ion exchange chromatography and isoelectric focusing. It was shown that MGIF is a nondialysable protein with a molecular weight of about 19,000 to 25,000. Charge heterogeneity was revealed by isoelectric focusing which demonstrated pI of 3.8--4.5 and 5.0--6.0. MGIF seems to differ from tumour cell-derived MIF detected in the same ascitic fluids.
D000645:Ammonium Sulfate; D000818:Animals; D002474:Cell-Free System; D007372:Interferons / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D008114:Liver Neoplasms, Experimental / Q000276:immunology* / Q000382:microbiology; D008222:Lymphokines / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D008222:Lymphokines; D007372:Interferons; D000645:Ammonium Sulfate