title: Monoclonal antibody against cytoplasmic lectins of Dictyostelium discoideum: cross-reactivity with a membrane glycoprotein, contact site A, and with E. coli beta-galactosidase and lac repressor.
abstract: Monoclonal antibodies were raised against two soluble, galactose-binding lectins from cells of Dictyostelium discoideum, discoidin I and II. These antibodies reacted not only with both discoidins, but also with a plasma membrane glycoprotein of aggregation competent cells, called contact site A, and with two carbohydrate-binding proteins of E. coli, beta-galactosidase and lac repressor. The possibility that the antibody recognizes a structure common to different carbohydrate-binding proteins is discussed. The two carbohydrate-binding proteins of E. coli share with discoidin I the sequence -Ser-X-X-Ile-His(Pro)-Pro(His)-Leu-Thr- which might be responsible for the cross-reactivity.
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004023:Dictyostelium / Q000032:analysis*; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D012097:Repressor Proteins / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase