The orientation of the ligands in iron(III)-bleomycin intercalated with DNA.
EPR data show that Fe(III)-bleomycin intercalates with DNA, or that the Fe(III) coordination sphere has a fixed geometrical configuration with respect to the DNA helical axis. An analysis of the data from oriented DNA fibers, drawn from a viscous gel, shows that the angle between the fiber axis and the normal to a plane containing the Fe(III) ion and ligands ranges between 15 and 30 degrees. The principal g values for the low-spin Fe(III)-bleomycin-DNA complex at pH 7.5 are 2.45, 2.18 and 1.87.
D001761:Bleomycin; D004247:DNA; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds; D007364:Intercalating Agents; D009690:Nucleic Acid Conformation
D005290:Ferric Compounds; D007364:Intercalating Agents; D001761:Bleomycin; D004247:DNA