Actinomycin D inhibition of monoclonal antibody binding to nucleolar phosphoprotein 37/5.2 (B23).
Actinomycin D was found to block binding of a monoclonal antibody to nucleolar phosphoprotein 37/5.2 (B23) when incubated either simultaneously with or prior to addition of the antibody. Daunorubicin had no significant blocking activity of this antigen-antibody reaction. In comparative studies with a monoclonal antibody to nucleolar phosphoprotein 110/5.2 (C23), actinomycin D exhibited little blocking activity. When a 42-mer peptide containing the epitope of protein 37/5.2 (B23) was tested as the antigen, similar inhibition by actinomycin D of binding of the monoclonal antibody was found. These results provide evidence for binding of actinomycin D to a specific epitope of protein 37/5.2 (B23).
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003609:Dactinomycin / Q000494:pharmacology*; D003630:Daunorubicin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D007163:Immunosorbent Techniques; D008099:Liver / Q000648:ultrastructure; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000276:immunology; D012261:Ribonucleoproteins / Q000276:immunology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012261:Ribonucleoproteins; C036825:phosphoprotein C23; D000090243:Nucleophosmin; D003609:Dactinomycin; D003630:Daunorubicin