Theophylline, dyphylline, caffeine, acetaminophen, salicylate, acetylsalicylate, procainamide, and N-acetylprocainamide determined in serum with a single liquid-chromatographic assay.
We describe a single set of liquid-chromatographic conditions for assay of theophylline, dyphylline, caffeine, acetaminophen, salicylate, acetylsalicylate, procainamide, and N-acetylprocainamide in serum. The chromatographic system includes a Waters Associates mu-Bondapak C18 column and acetonitrile in 0.1 mol/L potassium phosphate buffer, pH 4.0 (9.75/90.25 by vol), as the mobile phase. Only 50 microL of serum is required, and drug concentrations as low as 0.5 mg/L can be detected. Absolute and relative analytical recoveries range from 95 to 101%. Day-to-day variation of the method is less than 6% for each drug. Linearity extends to 1 g/L for all drugs. Recycling of the mobile phase under pressure eliminates the need to prepare and de-gas solvents. The use of the single stationary and mobile phase provides a practical and economical approach to routine and urgent therapeutic drug monitoring.
D000120:Acecainide / Q000097:blood; D000082:Acetaminophen / Q000097:blood; D001241:Aspirin / Q000097:blood; D002110:Caffeine / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004400:Dyphylline / Q000097:blood; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood*; D011342:Procainamide / Q000097:blood; D012459:Salicylates / Q000097:blood; D020156:Salicylic Acid; D013806:Theophylline / Q000097:blood
D004364:Pharmaceutical Preparations; D012459:Salicylates; D004400:Dyphylline; D000082:Acetaminophen; D002110:Caffeine; D000120:Acecainide; D013806:Theophylline; D011342:Procainamide; D020156:Salicylic Acid; D001241:Aspirin