A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS.
A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.
D000818:Animals; D001370:Axonal Transport; D001560:Benzidines / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology*; D002415:Cats; D002525:Cerebellar Cortex / Q000033:anatomy & histology; D006652:Histological Techniques; D006735:Horseradish Peroxidase / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D009412:Nerve Fibers / Q000648:ultrastructure; D009847:Olivary Nucleus / Q000033:anatomy & histology; D010544:Peroxidases / Q000378:metabolism; D011149:Pons / Q000033:anatomy & histology; D051381:Rats; D012012:Red Nucleus / Q000033:anatomy & histology; D012160:Retina / Q000033:anatomy & histology; D013116:Spinal Cord / Q000033:anatomy & histology; D013194:Staining and Labeling; D014795:Visual Pathways / Q000033:anatomy & histology; D014909:Wheat Germ Agglutinins
D001560:Benzidines; D037102:Lectins; D014909:Wheat Germ Agglutinins; C021758:3,3',5,5'-tetramethylbenzidine; D006735:Horseradish Peroxidase; D010544:Peroxidases