Contrasting effect of verapamil on catecholamine release induced by acetylcholine and by NaCl-omission from the bovine adrenal medulla.
Verapamil (3 X 10(-4) M) produced a 92.2% inhibition on the release of catecholamines induced by acetylcholine in perfused bovine adrenal glands. This blocking effect was slowly reversible. On the other hand, verapamil (3 X 10(-4) M) failed to antagonize the secretory response evoked by the substitution of NaCl by sucrose in the perfusion fluid. A similar lack of inhibitory effect of verapamil on catecholamine output produced by NaCl-omission was observed in the absence of extra-cellular Ca2+. These results suggest that Ca2+ influx might not be involved in adreno-medullary secretion induced by the substitution of NaCl by sucrose in the perfusion medium.
D000109:Acetylcholine / Q000037:antagonists & inhibitors*; D000313:Adrenal Medulla / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D003864:Depression, Chemical; D012965:Sodium Chloride / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology*
D002395:Catecholamines; D012965:Sodium Chloride; D013395:Sucrose; D014700:Verapamil; D000109:Acetylcholine; D002118:Calcium