A monoclonal antibody against an epitope common to HLA-b locus antigens.
A monoclonal antibody G4 that appears to be directed against a determinant common to all HLA-B locus antigens is described. This antibody reacted with a large panel of B and T lymphocyte and cell lines, but it did not react with two lines that do not serologically express HLA antigens (Daudi and K562) and two lines that expressed A-locus but not B-locus antigens (8402 and HPBMLT). The F(ab')2 fragment of G4 blocked B-locus but not A-locus HLA alloantisera. By immunoprecipitation and SDS-polyacrylamide gel electrophoresis G4 reacted with a dimer consisting of a heavy chain of 44,000 daltons and a light chain of 12,7000 daltons.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001667:Binding, Competitive; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D015235:HLA-B Antigens; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D000939:Epitopes; D006680:HLA Antigens; D015235:HLA-B Antigens; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G