Comparison of reversed-phase and weak anion-exchange high-performance liquid chromatographic methods for peptide separations.
Weak anion-exchange and reversed-phase high-performance liquid chromatographic methods for peptide separations were compared using a tryptic digest of "rat small myelin basic protein". In these experiments, a number of tryptic peptides that were not resolved on the reversed-phase column could be separated on the weak anion-exchange column, and in other instances, as might be expected, reversed-phase chromatography provided better resolution of certain peptides than did the weak anion-exchange method. The results obtained strongly suggest that the combined use of these two methods of separation, which utilize different selectivities, can provide an excellent improvement in resolving power for a number of peptide separations.
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D004676:Myelin Basic Protein / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D014357:Trypsin
D004676:Myelin Basic Protein; D010455:Peptides; D014357:Trypsin