Continuous production of maltooligosaccharides from glucose 1-phosphate by amylase-phosphorylase reactor.
Using an ultrafiltration cell, the continuous production of maltose and a mixture of other oligosaccharides was achieved by the combined action of alpha- or beta-amylase and phosphorylase. For the beta-amylase-phosphorylase system, a steady production of maltose was obtained until the beta-amylase was inactivated. For the alpha-amylase-phosphorylase system, it was difficult to maintain a stable production of oligosaccharides, probably due to the destruction of substrate glycogen by random cleavage by alpha-amylase.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D008722:Methods; D009132:Muscles / Q000201:enzymology; D009844:Oligosaccharides / Q000096:biosynthesis*; D010762:Phosphorylase a / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology; D011817:Rabbits; D000516:alpha-Amylases / Q000378:metabolism*; D001614:beta-Amylase / Q000378:metabolism*
D009844:Oligosaccharides; C021705:maltooligosaccharides; D010762:Phosphorylase a; D006005:Phosphorylases; D000681:Amylases; D000516:alpha-Amylases; D001614:beta-Amylase