Elemental redistribution and ultrastructural changes in guinea pig epidermis after dinitrochlorobenzene (DNCB) exposure.
Energy dispersive x-ray microanalysis and transmission electron microscopy were used to study ultrastructural and chemical changes occurring in the keratinocytes at the primary irritant dinitrochlorobenzene (DNCB) reaction. The results of the microanalysis showed a similarity between dose- and time-dependent effects of DNCB and were in accordance with the ultrastructural findings. With increasing cell injury (as judged by the ultrastructure) there was an increasing loss of phosphorus, potassium, and magnesium from the cells and an increase of cellular calcium content. Sodium and chloride were only moderately changed. Results recorded when the skin was exposed to a weak DNCB dose were in accordance with a stimulation of the basal keratinocyte cell population.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004137:Dinitrochlorobenzene / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004577:Electron Probe Microanalysis; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D006168:Guinea Pigs; D007633:Keratins / Q000032:analysis; D008274:Magnesium / Q000378:metabolism; D008297:Male; D009578:Nitrobenzenes / Q000494:pharmacology*; D010758:Phosphorus / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism*
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; D014131:Trace Elements; D010758:Phosphorus; D007633:Keratins; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium