Quantitation of protein and DNA in silver-stained agarose gels.
A silver stain for both proteins and DNA in agarose gels is described. Quantitation of proteins with this stain is possible, with individual proteins exhibiting characteristic responses, as observed with other stains. The advantage of the silver stain over Coomassie blue is its increased (50- to 100-fold) sensitivity, which allows samples containing very low protein concentrations to be analyzed without prior concentration. This silver stain, when applied to DNA, is at least as sensitive as ethidium bromide, and gives a linear response for the type of DNA and fragment sizes studied.
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000032:analysis*; D003720:Densitometry; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling
D011506:Proteins; D012394:Rosaniline Dyes; D012834:Silver; C024757:Coomassie brilliant blue R; D004247:DNA; D004996:Ethidium