[3H]spiroperidol binding in crude membrane suspensions of gill tissue from the marine mussel, Mytilus californianus.
Saturable, high-affinity binding sites for [3H]spiroperidol can be demonstrated in crude suspensions of mussel gill tissue. This binding shows stereospecificity toward the d- and l-isomers of butaclamol. Competition studies show that both dopamine and serotonin can displace [3H]spiroperidol from binding sites at nanomolar concentrations. Evidence is presented that suggests that the [3H]spiroperidol-binding sites can be divided into two distinct groups: those with high affinity for dopamine and those with high affinity for serotonin.
D000818:Animals; D001665:Binding Sites; D049872:Bivalvia / Q000378:metabolism*; D002069:Butaclamol / Q000378:metabolism; D002090:Butyrophenones / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D005880:Gills / Q000648:ultrastructure*; D012701:Serotonin / Q000378:metabolism; D013134:Spiperone / Q000378:metabolism*
D002090:Butyrophenones; D012701:Serotonin; D013134:Spiperone; D002069:Butaclamol; D004298:Dopamine