Polymorphic and monomorphic HLA-DR determinants on human hematopoietic progenitor cells.
The expression of monomorphic Ia-like antigens and polymorphic (allotypic) HLA-DR determinants on CFU-GM, BFU-E, CFU-E, and CFU-GEMM was studied in bone marrow and peripheral blood cells from normal healthy individuals. Using various polyclonal and monoclonal anti-Ia-like antibodies, the presence of HLA-DR backbone antigens was shown on all hematopoietic progenitor cells (HPC) studied, both in complement-dependent cytotoxicity assays and in fluorescence-activated cell sorting (FACS). The expression of allotypic determinants was demonstrated on all HPCs, using the HLA-DR typing sera anti-HLA-DR1, 2, 3, 4, 5, and 7. The Class II antigen MT-2 was also shown on all HPCs, using both monoclonal and alloantisera, whereas the MB-1 (DC-1) determinant could not be demonstrated on HPCs. This might open the possibility of removing MB-1-positive malignant cells from the graft in autologous bone marrow transplantation.
D000961:Antilymphocyte Serum / Q000494:pharmacology; D001667:Binding, Competitive; D003114:Colony-Forming Units Assay; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005434:Flow Cytometry; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D011110:Polymorphism, Genetic
D000961:Antilymphocyte Serum; D000939:Epitopes; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II