Incident light microscopy of normal and transformed cultured fibroblasts stained with coomassie blue R250.
Incident light microscopy of fibroblasts stained with Coomassie blue R250 results in images of the cytoskeleton with a similar high contrast to those obtained by specific immunofluorescence microscopy. This technique is especially useful for the routine visualization of the cytoskeleton of transformed cells.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts; D005354:Fibrosarcoma; D051379:Mice; D008853:Microscopy / Q000379:methods*; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R