Oxidized p-phenylenediamine staining of epoxy resin sections.
Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable electron opacity in PPD positive structures.
D000818:Animals; D001854:Bone Marrow Cells; D004853:Epoxy Resins; D005260:Female; D005976:Glutaral; D007420:Intestine, Large / Q000166:cytology*; D008297:Male; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D010084:Oxidation-Reduction; D010655:Phenylenediamines; D051381:Rats; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology*; D014059:Tongue / Q000166:cytology*; D014599:Uterus / Q000166:cytology*
D004853:Epoxy Resins; D010655:Phenylenediamines; D005976:Glutaral; C029728:4-phenylenediamine