Production of interferon-gamma in the syngeneic mixed lymphocyte reaction.
Cocultures of nylon wool-separated, non-adherent (T enriched) mouse spleen cells with mitomycin C-treated adherent cells resulted in the production of antiviral activity in the culture supernatants. This antiviral activity was defined as interferon gamma (IFN-gamma) because of its lability at pH 2 and the susceptibility to inactivation by anti mouse IFN-gamma antibodies. Treatment of non-adherent cells with anti-Thy 1.2 or anti-Lyt 1.2 antibody plus complement abolished IFN production. In contrast, anti-Lyt 2.2 plus complement treatment had no effect on IFN production. Abrogation of IFN production was also seen when anti-Ia antibodies were added. IFN could be observed in the SMLR, regardless of the H-2 haplotype of the mouse strains with the exception of NZB mice. Addition of hydrophilic polymers such as polyethyleneglycol or DEAE-dextran enhanced IFN production.
D000818:Animals; D001790:Blood Physiological Phenomena; D003911:Dextrans / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000502:physiology; D007700:Kinetics; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D010641:Phenotype; D011092:Polyethylene Glycols / Q000494:pharmacology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D003911:Dextrans; D011092:Polyethylene Glycols; D007371:Interferon-gamma