Detection of lipopolysaccharides in polyacrylamide gels by transfer to nitrocellulose followed by immunoautoradiography with antibody and 125I-protein A: "LPS blotting".
Lipopolysaccharides (LPS), which constitute the somatic (O) antigen of gram-negative bacteria, were used to demonstrate the procedure of LPS blotting involving the electrophoretic transfer of electrophoretically resolved LPS from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose filters. Immobilized LPS could then be immunoautoradiographically visualized in situ by reaction with specific anti-LPS antibody and subsequent binding of radioiodinated Staphylococcus protein A. LPS blotting is expected to provide an efficient and specific means of investigating the LPS (O) antigens of gram-negative bacteria.
D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D001345:Autoradiography; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D011863:Radioimmunoassay; D012834:Silver; D013194:Staining and Labeling; D013205:Staphylococcal Protein A / Q000276:immunology
D000907:Antibodies, Bacterial; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D013205:Staphylococcal Protein A; D012834:Silver; D003101:Collodion