title: Regulation of synthesis of the larval serum proteins of Drosophila melanogaster.
abstract: We have determined the relative amounts of subunits of larval serum proteins (LSPs) 1 and 2 during larval development in Drosophila melanogaster. These results indicate that synthesis of polypeptide subunits of LSP-1 and LSP-2 is coordinate: the proteins are first detected at the same time; they accumulate in a coordinate fashion; their RNAs are first detected at the same time; the RNAs also accumulate in similar relative amounts. Analyses of fat body polypeptides and fat body RNA indicate that synthesis of LSP-1 declines at a time when there are still substantial quantities of LSP-1 RNA in the cytoplasm. Cessation of LSP-1 subunit synthesis occurs before cessation of LSP-2 synthesis, indicating that at late times the genes (or mRNAs) for these two proteins are subject to different "switch-off" controls.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism*; D007301:Insect Hormones / Q000235:genetics* / Q000302:isolation & purification; D007814:Larva / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; C034648:larval serum protein, Drosophila; D012313:RNA; D008715:Methionine