title: Inhibition of rat alpha-fetoprotein-estrogen interaction by adult rat ovarian extracts--role of unsaturated fatty acids.
abstract: Thin layer chromatography with four different solvent systems enabled us to show that ovarian extracts contained nonesterified unsaturated fatty acids able to compete with estradiol on the alpha-fetoprotein binding site. Gas chromatography demonstrated a high level of oleic and linoleic acids and the presence of arachidonic acid, a strong competitor of the rat alpha-fetoprotein-estrogen interaction. Arachidonic acid is a precursor of prostaglandins thus we suggest that its binding to AFP might play a role in the AFP-mediated ovarian regulation previously demonstrated.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D010053:Ovary / Q000032:analysis*; D051381:Rats; D000509:alpha-Fetoproteins / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D000509:alpha-Fetoproteins; D004958:Estradiol