title: Pulse and steady-state labelled and actinomycin D chased 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)-resistant hnRNA from uninfected HeLa cells.
abstract: The fraction of hnRNA synthesized in the presence of DRB in uninfected HeLa cells ranges in size from 4S to over 45S. High molecular weight DRB-resistant hnRNA has been demonstrated to decay after 2 h of actinomycin D chase. This fraction is composed of both poly(A)(+) and poly(A)(-) RNA molecules. The synthesis in the presence of DRB of short polyadenylated hnRNA was also observed. The nature of both hnRNA subfractions is discussed.
mesh_terms: D003609:Dactinomycin / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology*; D004351:Drug Resistance; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis*; D012333:RNA, Messenger; D012263:Ribonucleosides / Q000494:pharmacology*
chemical_list: D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D012263:Ribonucleosides; D003609:Dactinomycin; D011061:Poly A; D004004:Dichlororibofuranosylbenzimidazole; D012313:RNA