Part of the 23S RNA located in the 11S RNA fragment is a constituent of the ribosomal peptidyltransferase centre.
Upon irradiation, 3-[4-benzoylphenyl]propionyl-PhetRNA bound to the P-site of poly(U)-primed ribosomes is exclusively cross-linked to 23S RNA. It is shown that the photoreaction only occurs with pyrimidine nucleotides. The site of the cross-link is located within an 11S RNA fragment, which comprises the 1100 nucleotides at the 3'-end of 23S RNA. The cross-linked Phe tRNA derivative is still functionally active in peptide bond formation. The site labelled on the 11S fragment is therefore an integral part of the peptidyltransferase centre.
D000217:Acyltransferases / Q000302:isolation & purification*; D000345:Affinity Labels; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000235:genetics; D010458:Peptidyl Transferases / Q000302:isolation & purification*; D010777:Photochemistry; D011072:Poly U / Q000032:analysis; D012329:RNA, Bacterial / Q000302:isolation & purification / Q000528:radiation effects; D012335:RNA, Ribosomal / Q000302:isolation & purification* / Q000528:radiation effects; D012346:RNA, Transfer, Amino Acyl
D000345:Affinity Labels; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012346:RNA, Transfer, Amino Acyl; C039971:tRNA, 3-(4-benzoylphenyl)propionyl-phenylalanine-; D011072:Poly U; D000217:Acyltransferases; D010458:Peptidyl Transferases