In vivo study of the influence of adenine and guanosine on the erythrocytes of ACD (pH 5.0 and 5.6) preserved blood.
Data are presented for percentage of recovery, survival time (T1/2) and mode of sequestration of erythrocytes from ACD [disodium citrate 95 mmol/l and glucose (C6H12O6 X H2O)] 152 mmol/l or ACD--adenine or adenine + guanosine (pH ranging from 5.0 to 5.6) preserved blood for 35 days at 4-8 degrees C (277-281 K). With the availability of guanosine in 0.25 mmol/l or 0.5 mmol/l final concentration in ACD + 0.25 or 0.5 mmol/l adenine preserved blood a positive effect can be exerted on erythrocyte 24 hrs recovery and survival time (T1/2). This effect is particularly evident when pH of the preservative solution is raised to 5.6. Final concentrations of 0.25 mmol/l adenine and guanosine in ACD preserved blood (whole or packed erythrocytes, pH 5.6, Hct. 0.73 or 0.61) are sufficient to ensure 35 days of storage at 4-8 degrees C (277-281 K).
D000225:Adenine / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002860:Chromium Radioisotopes; D002951:Citrates; D019343:Citric Acid; D004905:Erythrocyte Aging; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000187:drug effects*; D005947:Glucose; D006151:Guanosine / Q000494:pharmacology*; D006801:Humans
D002860:Chromium Radioisotopes; D002951:Citrates; D006151:Guanosine; D019343:Citric Acid; D005947:Glucose; D000225:Adenine