title: Variability in the distribution of callosal projection neurons in the adult rat parietal cortex.
abstract: Previous reports have shown that the barrel field area of the parietal cortex of the adult rat contains relatively few callosal projection neurons, even though callosal projection neurons are abundant in this cortical region in the neonatal rat. Furthermore, it has been shown that many of the callosal neurons which seem to disappear as the animal matures do not die, but project to ipsilateral cortical areas. These findings rely on the ability of retrograde transport techniques which utilize injections of horseradish peroxidase (HRP) or of fluorescent dyes into one hemisphere. We now show that several technical modifications of the HRP technique yield a wider distribution of HRP-containing neurons in the contralateral barrel field area of the adult rat than previously reported. These include implants of HRP pellets into transected axons of the corpus callosum, the addition of DMSO and nonidet P40 to Sigma VI HRP, wheat germ agglutinin HRP and the use of tetramethyl benzidine as the chromogen in the reaction procedure. Our findings have implications for transport studies in general and for the development of the cortical barrel field in particular.
mesh_terms: D000818:Animals; D001560:Benzidines; D002863:Chromogenic Compounds; D003337:Corpus Callosum / Q000033:anatomy & histology*; D004121:Dimethyl Sulfoxide; D014644:Genetic Variation; D006735:Horseradish Peroxidase; D037102:Lectins; D009434:Neural Pathways / Q000033:anatomy & histology; D017830:Octoxynol; D010296:Parietal Lobe / Q000033:anatomy & histology*; D011092:Polyethylene Glycols; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D014909:Wheat Germ Agglutinins
chemical_list: D001560:Benzidines; D002863:Chromogenic Compounds; D037102:Lectins; D014909:Wheat Germ Agglutinins; C021758:3,3',5,5'-tetramethylbenzidine; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D006735:Horseradish Peroxidase; D004121:Dimethyl Sulfoxide