Monoclonal antibodies against Vibrio cholerae lipopolysaccharide.
A cell line producing monoclonal antibodies directed against the core region of Vibrio cholerae lipopolysaccharide has been established. These antibodies were inhibited by lipopolysaccharide preparations of both O-group 1 vibrios and some non-O-group 1 vibrios as detected in enzyme-linked immunosorbent assay-inhibition experiments. Coagglutination experiments with monoclonal and polyclonal antibodies adsorbed to protein A-carrying staphylococci were performed. All V. cholerae strains tested, regardless of serotype, were agglutinated when mixed with staphylococci coated with the monoclonal antibodies, whereas staphylococci coated with group-specific (O1) polyclonal antibodies only agglutinated with O-group 1 vibrios.
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D008070:Lipopolysaccharides