Detection of protein in polyacrylamide gels using an improved silver stain.
A much improved silver staining procedure for the detection of protein in polyacrylamide gels of 0.8-3.0 mm thickness is described. It achieves very high sensitivity (detecting less than 0.01 ng bovine serum albumin/mm2) by overstaining and subsequently removing nonspecific background stain using a modified, reliable destaining procedure. Maximum sensitivity follows prediamine equilibration in 0.1% (w/v) formaldehyde solution. With two-dimensional electrophoresis the improved staining procedure reveals greater than 200 polypeptides in unconcentrated human urine and greater than 150 polypeptides in a single human fingerprint.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing; D011506:Proteins / Q000032:analysis*; D012834:Silver; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D012834:Silver