Delineation of a cross-reactive idiotype on human autoantibodies with antibody against a synthetic peptide.
Antibody against a cross-reactive idiotype (CRI) on human IgM-rheumatoid factor (RF) antibodies was induced by immunization of rabbits with a synthetic peptide ( PSL2 ) corresponding to the second complementarity-determining region (CDR), and adjacent amino acid residues of the kappa light chain of the IgM-RF Sie . The anti-peptide antibody bound efficiently to IgM-RF proteins known to share a cross-reactive idiotype, and to their isolated kappa chains. The anti-CRI was absorbed by, and eluted from, a peptide-Sepharose affinity column. The antibody activity was inhibited by the free peptide in solution. The anti-peptide antibody thus identifies a public idiotype on human IgM-RF, that is largely dependent on the primary sequence of the second CDR of the light chain. Such peptide-induced antiidiotypes of predefined specificity may facilitate studies of the molecular basis of idiotypic cross-reactions, the inheritance and somatic diversification of antibody molecules, and the regulation of the idiotype network.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000502:physiology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D011817:Rabbits; D012217:Rheumatoid Factor / Q000096:biosynthesis / Q000276:immunology* / Q000502:physiology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D010455:Peptides; D012217:Rheumatoid Factor