A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution.
A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.
D001345:Autoradiography; D001482:Base Composition; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004247:DNA / Q000032:analysis; D004277:DNA, Single-Stranded; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae
D003573:Cytochrome b Group; D004277:DNA, Single-Stranded; D012313:RNA; D004247:DNA