title: Tissue culture of human cholesteatomatous keratinocytes.
abstract: The aim of this study was to culture keratinocytes from migratory skin and from cholesteatomatous matrix. At operation, cholesteatomatous matrix, normal drumhead and non-migratory stratifying skin from the extrameatal incision were obtained. Successful cultures of keratinocytes were produced in 4 out of 5 attempts. The cholesteatomatous keratinocytes produced slower growing colonies with an abnormal pattern of keratinization, whereas the drumhead produced rapidly growing colonies with a more regular pattern of keratinization, similar to those obtained from a vertically stratifying epidermis. This method may offer new fields of study in the investigation of cholesteatoma.
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002781:Cholesteatoma / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D046508:Culture Techniques / Q000379:methods*; D004428:Ear Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004432:Ear, Middle; D006801:Humans; D007633:Keratins / Q000378:metabolism*
chemical_list: D007633:Keratins