An automated, affinity-column-mediated, enzyme-linked immunometric assay for digoxin on the Du Pont aca discrete clinical analyzer.
We describe an automated assay for digoxin that requires a 200-microL sample of serum. Total analysis time is 18 min. The method is extremely precise, with within-run CVs of 2.6, 1.6, and 4.9%, respectively, at 0.5, 1.5, and 3.5 micrograms of digoxin per liter (n = 20). The lower limit of detection is 0.2 micrograms of digoxin per liter. For patients' samples, the correlation with RIA (x) is excellent (r = 0.95; y = 0.95x - 0.14; standard error = 0.17 micrograms/mL). We saw no interferences in samples having high concentrations of rheumatoid factors, lipid, bilirubin, or hemoglobin. Cross reactivity with digoxin analogs and steroidal compounds is similar to that observed by RIA.
D001322:Autoanalysis / Q000295:instrumentation; D002846:Chromatography, Affinity; D003429:Cross Reactions; D003911:Dextrans; D004077:Digoxin / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007140:Immunoglobulin Fab Fragments; D010042:Ouabain; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D001616:beta-Galactosidase
D003911:Dextrans; D007140:Immunoglobulin Fab Fragments; D012710:Serum Albumin, Bovine; D010042:Ouabain; D004077:Digoxin; C025614:sephadex; D001616:beta-Galactosidase