A monoclonal antibody demonstrating specificity for drug-resistance cells.
We have described a monoclonal antibody with specificity for permeability-altered drug-resistant cells. This gamma G2b antibody was characterized by antibody-mediated complement-dependent cytotoxicity and by immunofluorescence analysis using a flow cytometer-cell sorter. Further experiments with the flow cytometer showed that vinblastine=resistant cells exposed to that drug had a wider range of fluorescence intensity and had a modal channel 4 times as bright as the same cells cultured in growth medium. We believe that this monoclonal antibody recognizes part of the "P' glycoprotein found on drug-resistant Chinese hamster ovary cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D004351:Drug Resistance; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D010641:Phenotype; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000939:Epitopes