[Structuro-functional properties of the bacteria Bacillus brevis in relation to the accumulation of gramicidin S in cells].
The culture of Bacillus brevis var. G-B R-form was grown in the presence of beta-phenyl-beta-alanine, the inhibitor of gramicidin S synthesis, is characterized by enhanced endogenous respiration and the DPI-reductase activity as compared to the culture synthezising antibiotic. The increased synthesis of the antibiotic in the region of the culture transition from the logarithmic growth phase to the linear one is associated with a decrease in the number of viable cells despite the fact that the culture on the whole does not die but continues to grow. The membranes prepared from young gramicidin S-free cells and from the cells enriched with the antibiotic possess identical electron micrograph images, IR spectra and protein sets as determined by polyacrylamide gel electrophoresis in a Na-DS system. However, in young cell membranes NADH and succinate dehydrogenase are insensitive to gramicidin S and only malate dehydrogenase is inhibited by this antibiotic. In aged cell membranes the activities of all mentioned dehydrogenases are suppressed. Malate dehydrogenase from young cells is weakly inhibited by thyrotrycin obtained from Bac. brevis ATCC 10068; succinate dehydrogenase is entirely insensitive to this antibiotic, while NADH-dehydrogenase is almost completely inhibited by it. The specificity of action on the respiratory chain of peptide antibiotics synthesized by the cells of one strain of Bac. brevis is suggestive of a possible regulatory role of these peptides in the metabolism of the producent. Hence the accumulation of gramicidin S which is adsorbed on the membrane and destroys the respiratory chain function to the cause of the low rate of oxygen uptake by the culture of Bac. brevis var. G-B R-form and of the low activities of DPI-reductases.
D000409:Alanine / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D006096:Gramicidin / Q000096:biosynthesis*; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors; D010101:Oxygen Consumption; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology*
C024799:2-phenyl-3-aminopropanoic acid; D015091:beta-Alanine; D006096:Gramicidin; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D000409:Alanine
Izuchenie strukturno-funktsional'nykh osobennosteĭ bakteriĭ Bacillus brevis v sviazi s nakopleniem v kletakh gramitsidina S.