title: Mouse monoclonal IgM antibody against human lung cancer line SK-LC-3 with specificity for H(O) blood group antigen.
abstract: Spleen cells from mice immunized with the human lung cancer line SK-LC-3 were fused with mouse NS-1 myeloma cells. One of the hybrid clones produced a monoclonal IgM antibody (designated F-3), detected with antimouse Ig-MHA and hemagglutination assays. This antibody was completely absorbed by O red cells and completely inhibited by low concentrations of H(O) glycoproteins and hog mucin (A + H). Bombay (Oh) red cells completely failed to absorb F-3 activity even after treatment with neuraminidase. A1, A2, A1B, A2B, and B red cells and A- and B-glycoproteins were less effective in absorbing or inhibiting F-3 activity. Other glycoproteins (including those having Lea or blood group precursor structures) showed little or no inhibitory activity. Serum from nude mice carrying F-3 hybridoma agglutinated O and A2 red cells at a titer of 1:40,000 and 1:640, respectively. A1, A1B, A2B, and B red cells were agglutinated with titers of 1:80 or less. Monoclonal antibody F-3 is, therefore, highly specific for H(O) blood group determinants.
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D001789:Blood Group Antigens / Q000276:immunology; D002460:Cell Line; D000939:Epitopes; D005752:Gastric Mucins / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology*; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D011134:Polysaccharides / Q000276:immunology; D012463:Saliva / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D001789:Blood Group Antigens; D000939:Epitopes; D005752:Gastric Mucins; D006023:Glycoproteins; D007075:Immunoglobulin M; D011134:Polysaccharides; D009439:Neuraminidase