title: alpha 2-Macroglobulin-proteinase complexes in cystic fibrosis serum. Analysis by monoclonal antibodies and receptor-mediated endocytosis by normal human fibroblasts.
abstract: alpha 2-Macroglobulin complexed to proteinases activated during clotting of cystic fibrosis and control sera was quantitated with the complex-specific monoclonal antibody F2B2 . Similar amounts of alpha 2-macroglobulin complexes (between 40 and 90 micrograms/ml) were generated in cystic fibrosis and control sera. Endocytosis of the complexes by normal human fibroblasts was compared to the amount of complexes detected by the F2B2 -radioimmunoassay. Normal uptake was observed with 13 out of 14 cystic fibrosis sera. One cystic fibrosis serum showed strongly reduced endocytosis of the complexes. Complexes isolated from this serum on immobilized F2B2 failed to inhibit binding of purified alpha 2-macroglobulin-trypsin to its receptor, demonstrating deficient receptor-binding of these complexes. The low uptake complexes could not be distinguished from complexes isolated from control or other cystic fibrosis sera by isoelectric focusing, rate electrophoresis or SDS-polyacrylamide gel electrophoresis.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003550:Cystic Fibrosis / Q000097:blood*; D004705:Endocytosis; D010450:Endopeptidases / Q000097:blood*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D011971:Receptors, Immunologic / Q000502:physiology*; D000511:alpha-Macroglobulins / Q000032:analysis / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D046911:Macromolecular Substances; D011971:Receptors, Immunologic; D000511:alpha-Macroglobulins; D010450:Endopeptidases