Validation of the common-core hypothesis of estrogen receptors with precipitating and steroid-releasing antibodies.
The immunoglobulin G fraction of a goat antiserum raised against a presumed endoglycosidase-fission product of estradiol receptor from porcine uteri contains some antibodies which precipitate the estradiol/receptor complex and others which release the steroid from the protein. Subcellular receptor forms and receptors from different porcine target organs react in the same fashion as do human, ovine, bovine, guinea pig, rabbit and rat estradiol receptors.
D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D003429:Cross Reactions; D003600:Cytosol / Q000378:metabolism; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011960:Receptors, Estrogen / Q000276:immunology / Q000378:metabolism*; D012756:Sheep; D013552:Swine; D014599:Uterus / Q000378:metabolism
D000939:Epitopes; D007074:Immunoglobulin G; D011960:Receptors, Estrogen