Antigen-specific proliferative human T cell clones with specificity for diphtheria toxoid: genetic and molecular restriction by class II antigens.
Human T lymphocyte clones (TLC) specific for diphtheria toxoid (DT) were isolated from a DR6/7 individual by cloning in soft agar in vivo sensitized T lymphocytes. We report here the isolation and characterization of 3 of these clones by studying: (a) the kinetic of activation, (b) the surface phenotypes, (c) the fine specificity for one of the 2 DT chains and (d) the genetic restriction of the proliferative response by the haplotype DR7. Moreover, blocking studies of the proliferative response to DT by various immunochemically characterized anti-HLA-DR monoclonal antibodies indicate that, on the DR7 molecule, more than one Ia determinant may participate in the clonal DT proliferative response. By using human TLC of a defined specificity and well-characterized anti-DR monoclonal antibodies, such studies may help to define the functional repertoire of Ia molecules in man.
D002999:Clone Cells; D004168:Diphtheria Toxoid / Q000276:immunology*; D000939:Epitopes; D006684:HLA-DR Antigens; D006238:Haploidy; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006801:Humans; D007700:Kinetics; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D004168:Diphtheria Toxoid; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II