title: Silver staining of cell bodies by means of physical development.
abstract: A method for silver impregnation of cell bodies is detailed. It is based on Gallyas' physical development procedure, and adds a silver stain for cell bodies to the list of histological elements stainable with high contrast by means of physical development. Its black pigment on a clear background aids visibility, localization of cytoarchitectonic boundaries, and photographic reproduction. It also stains regions of tissue damage, such as tracks from micropipettes or electrodes. A manipulation for enhancement of dendritic staining is also described.
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D002540:Cerebral Cortex / Q000033:anatomy & histology; D006224:Cricetinae; D003712:Dendrites / Q000648:ultrastructure; D006624:Hippocampus / Q000033:anatomy & histology; D006652:Histological Techniques; D008297:Male; D008647:Mesocricetus; D009474:Neurons / Q000648:ultrastructure; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods*; D013477:Superior Colliculi / Q000033:anatomy & histology
chemical_list: D012835:Silver Nitrate