title: Simultaneous demonstration of UV-type and ionizing radiation-type DNA repair by the nucleoid sedimentation technique.
abstract: The nucleoid sedimentation technique is one of the most sensitive methods for measuring DNA excision repair. With this technique, we have shown that both UV- and ionizing radiation-type repair (the latter induced by bleomycin) can be discriminated in HeLa and normal diploid cells using 1-beta-D-arabinofuranosylcytosine. The latter compound inhibits UV-type repair synthesis, and thus causes DNA breaks due to enzymic incision to persist, but has no effect on rejoining DNA after ionizing radiation-type damage. It was then possible to prove that 4-nitroquinoline-1-oxide induces both types of lesions which are repaired simultaneously. This effect could be demonstrated in HeLa and normal human diploid cells in a single experimental set-up.
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D001761:Bleomycin / Q000633:toxicity; D002467:Cell Nucleus / Q000502:physiology / Q000528:radiation effects*; D003561:Cytarabine / Q000633:toxicity; D004260:DNA Repair / Q000528:radiation effects*; D006367:HeLa Cells / Q000502:physiology / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D014466:Ultraviolet Rays
chemical_list: D003561:Cytarabine; D001761:Bleomycin; D015112:4-Nitroquinoline-1-oxide