title: Immunohistochemical analysis of thyroglobulin and keratin in benign and malignant thyroid tumours.
abstract: 56 thyroid gland tumours and non neoplastic alterations were studied for keratin and thyroglobulin staining, using the indirect immunoperoxidase method on serial formalin fixed paraffin embedded sections. Papillary carcinomas showed a strong reaction with anti-keratin serum but a weak reaction with anti-thyroglobulin serum. Follicular adenomas and carcinomas showed virtually no reaction for keratin but a strong reaction for thyroglobulin. Undifferentiated and medullary carcinomas did not react with either antiserum, except for single cells in two undifferentiated carcinomas which reacted with anti-keratin serum. In nodular goiters, hyperplastic follicles showed little or no reaction with anti-keratin serum and strong reaction with anti-thyroglobulin serum. It is suggested that this virtually type-specific staining for keratin or thyroglobulin may be related to different degrees of cellular differentiation and organelle content in the tumour cells.
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis; D002277:Carcinoma / Q000175:diagnosis; D002291:Carcinoma, Papillary / Q000175:diagnosis; D003937:Diagnosis, Differential; D006044:Goiter, Nodular / Q000175:diagnosis; D006111:Graves Disease / Q000175:diagnosis; D006651:Histocytochemistry; D006801:Humans; D007106:Immune Sera; D007120:Immunochemistry; D007633:Keratins / Q000032:analysis* / Q000276:immunology; D013954:Thyroglobulin / Q000032:analysis* / Q000276:immunology; D013959:Thyroid Diseases / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000032:analysis* / Q000175:diagnosis; D013966:Thyroiditis / Q000175:diagnosis
chemical_list: D007106:Immune Sera; D007633:Keratins; D013954:Thyroglobulin