Tumor and histocompatibility antigen interaction: direct visualization by a double-label bridging technique for immuno-electron microscopy.
The nature of the interaction between the Ib tumor-associated surface antigen (Ib-TASA) and the Dk and Kk (Dk/Kk) regions of the major histocompatibility complex on the surface of line Ib lymphocytes was evaluated by immuno-electron microscopy using a double-label bridging technique. Quantitation of antigenic sites by direct counts showed 44% of the Ib-TASA sites coincident with Dk/Kk antigens. This is greater than the predicted random interaction of two independent cell surface determinants suggesting an associative interaction, i.e. 'altered-self' antigen. The remaining 56% of the Ib-TASA sites were in a non-associative mode. On the basis of data presented here, we postulate that the interaction between 'self'- and 'non-self' antigens is not an 'all or none' phenomenon.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000032:analysis / Q000276:immunology*; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes; D006649:Histocompatibility Antigens