title: Inhibition of Balbiani ring transcription following differential arrest of Balbiani ring-coded translation.
abstract: A differential inhibition of the synthesis of secretory proteins, mainly fractions formed in giant translation units, can be obtained in Chironomus salivary gland cells with low concentrations of the ribosome translocation inhibitor, cycloheximide with or without emetine. Both treatments also lead to puff regression and inhibition of transcription specific to the large Balbiani rings, BR1 and BR2, the loci for the giant secretory proteins. The amount of 75S BR RNA transcribed is also reduced in the cytoplasm and in the poly(A) RNA relative to other transcripts. The half-life of 75S RNA is, however, prolonged so that there is little if any decrease in the cytoplasmic content of 75S RNA. The effect on the Balbiani rings may be due to control emanating from the translational process.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002683:Chironomidae / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004640:Emetine / Q000494:pharmacology*; D007814:Larva / Q000502:physiology; D008970:Molecular Weight; D009705:Nucleosides / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D012469:Salivary Glands / Q000187:drug effects / Q000502:physiology; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000596:Amino Acids; D009705:Nucleosides; D011506:Proteins; D012313:RNA; D003513:Cycloheximide; D004640:Emetine