title: The oxidative generation of sulfonic acid groups in neuromelanin and lipofuscin in the human brain.
abstract: Sulfonic acid groups were oxidatively generated in the soluble lipid-free lipofuscin component of neuromelanin of human substantia nigra and in lipofuscin of human inferior olive. Exposure of these oxidized, intraneuronal pigments to low pH Alcian blue or aldehyde fuchsin demonstrated an intensity of staining that related to the type of oxidant and the conditions of its use. Utilization of the following oxidants generated increasingly strong staining reactions as signified by the following sequence; periodic acid under mild conditions, bromine in carbon tetrachloride, hydrogen peroxide, periodic acid under drastic conditions, potassium permanganate followed by oxalic acid, hydrogen peroxide followed by bromine in carbon tetrachloride, potassium permanganate followed by metabisulfite or bisulfite, and performic acid. Neither Alcian blue nor aldehyde fuchsin revealed oxidatively generated aldehyde as judged by 1) their failure or near failure to stain inferior olive lipofuscin following mildly applied periodic acid, and 2) the increase in staining intensity, from moderate to strong, displayed by the soluble lipid-free lipofuscin component of neuromelanin and by inferior olive lipofuscin when potassium permanganate was followed by a rinse with metabisulfite or bisulfite in place of one with oxalic acid.
mesh_terms: D001921:Brain / Q000378:metabolism*; D001966:Bromine; D005561:Formates; D006801:Humans; D006861:Hydrogen Peroxide; D008062:Lipofuscin / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D009847:Olivary Nucleus / Q000378:metabolism; D010504:Periodic Acid; D011196:Potassium Permanganate; D013194:Staining and Labeling; D013378:Substantia Nigra / Q000378:metabolism; D013451:Sulfonic Acids / Q000096:biosynthesis*
chemical_list: D005561:Formates; D008062:Lipofuscin; D008543:Melanins; D013451:Sulfonic Acids; C014121:neuromelanin; D011196:Potassium Permanganate; D010504:Periodic Acid; C008817:peroxyformic acid; D006861:Hydrogen Peroxide; D001966:Bromine