Localization of antigenic determinants in whole Drosophila embryos.
Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations.
D000818:Animals; D000911:Antibodies, Monoclonal; D004331:Drosophila melanogaster / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000276:immunology; D000939:Epitopes; D005404:Fixatives; D005455:Fluorescent Antibody Technique
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005404:Fixatives