A new technique for localization of cellulase in situ using silver nitrate.
A new staining technique has been developed for the histochemical localization of cellulase in plant tissues by light microscopy. The products of cellulolysis are reducing sugars which can reduce the salts of heavy metals under appropriate conditions. The present technique relies on the deposition of black silver oxide due to reduction of alkaline silver nitrate to detect cellulase in tissues.
D002480:Cellulase / Q000032:analysis*; D010944:Plants / Q000201:enzymology*; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods*
D012835:Silver Nitrate; D002480:Cellulase