Indication of a certain molecular correlation between rat IgE and human IgE.
The PCA-activity of rat sera sensitized with Ascaris suum antigen can be absorbed by antihuman IgE and by rabbit or sheep antihuman IgE convalently bound to sephadex particles or paper discs respectively. In vitro, rat immune sera show positive results using the human RIST-system abolished by preheating for 2 hours at 56 degrees C and therefore excluding an interaction of IgG 4. In contrast, no rat sera activity was seen using the human PRIST-system. These results agree with the concept of a single common antigenic determinant in human and rat IgE since in the human RIST and PRIST-system the binding of rat IgE to antihuman IgE could only be demonstrated on the principle of competitive inhibition (RIST) but not on that of a sequential binding (PRIST). The possibility of a certain heterogeneity in the IgE population can not be excluded.
D000818:Animals; D000941:Antigens / Q000276:immunology; D001200:Ascaris / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D010323:Passive Cutaneous Anaphylaxis; D011864:Radioimmunosorbent Test; D051381:Rats / Q000276:immunology*; D011919:Rats, Inbred Strains; D013045:Species Specificity
D000941:Antigens; D000939:Epitopes; D007073:Immunoglobulin E