title: Keratin and polyamide-coated inorganic matrices as supports for glucoamylase immobilization.
abstract: Previously solubilized feather keratin and polyamide were used for coating sand, glass beads and silica gel. These new seven supports were employed for comparative studies on pure glucoamylase / EC 3.2.1.3 / immobilization. The immobilization yield of glucoamylase on keratin and polyamide coated supports was comparable with conventional matrices used earlier. The highest activity per 1 g of support was shown by the enzyme bound to polyamide-coated CPG, and the bests operational stability by the enzyme immobilized on polyamide-coated CPG with keratin subsequently deposited on it.
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism; D005898:Glass; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D007633:Keratins; D009757:Nylons; D012995:Solubility
chemical_list: D004800:Enzymes, Immobilized; D009757:Nylons; D007633:Keratins; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase