Mediation of vascular endothelial proliferation by factors in fetal bovine retinal extracts and serum.
The effect of fetal bovine retinal extract (FBR) and dialyzed fetal bovine serum (FBS) on in vitro proliferation of vascular endothelial cells was studied. Quantitatively, FBR, both dialyzable and nondialyzable, and dialyzed FBS exhibited a synergism in bringing about an enhanced proliferation of vascular endothelial cells. Assays on selected samples using both aortic and retinal vascular endothelial cells yielded similar results. In the absence of dialyzed FBS, FBR had only a negligible stimulatory effect. When compared at a protein concentration of 3 mg ml-1, dialyzed FBS elicited vascular endothelial proliferation at a rate only 25% that of FBS (not dialyzed). However, under experimental conditions using 3 mg ml-1 of dialyzed FBS and 0.5 mg ml-1 of dialyzed retinal extract, a six-fold increase in the activity of vascular endothelial proliferation was demonstrated. The observed synergistic effect was dependent on the concentrations of both dialyzed FBS and FBR. The observed synergism rules out the possibility that the stimulatory effect of FBR was due to blood contamination in sample preparation. Qualitatively, major components of dialyzed FBS and dialyzed FBR that exerted a synergistic effect on the stimulation of vascular endothelial proliferation were isolated by permeation chromatography using Sephadex G-100, with Mr estimated at 150 000 and 80 000, respectively. One minor component in FBS and one in FBR were also present.
D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004727:Endothelium / Q000166:cytology; D005312:Fetal Blood; D005333:Fetus; D006133:Growth Substances / Q000494:pharmacology*; D009389:Neovascularization, Pathologic / Q000473:pathology; D012039:Regional Blood Flow; D012160:Retina / Q000098:blood supply / Q000196:embryology; D014020:Tissue Extracts / Q000494:pharmacology*
D006133:Growth Substances; D014020:Tissue Extracts