Effect of dextran-coated charcoal on radioimmunoassays of unconjugated estriol in serum.
Lipid interference in the radioimmunoassay of steroids can cause an apparent variation in post-prandial serum concentrations of unconjugated estriol. The effects of increased concentrations of dextran-coated charcoal (15--60 g/l) in the radioimmunoassay of estriol standards with and without triglycerides were studied. All concentrations of dextran-coated charcoal used in this study eliminated the previously observed effects of estriol-lipid partitioning. When we used similar concentrations of dextran-coated charcoal, we found that serum cortisol levels in pregnant women declined significantly during the day (P less than 0.001), but no significant variation in estriol concentrations was observed. These results indicate that the previously reported diurnal variation in serum unconjugated estriol concentrations attributed to steroid-lipid partitioning can be eliminated by increasing the concentration of dextran-coated charcoal in assays using this method of separation.
D002606:Charcoal; D002940:Circadian Rhythm; D003911:Dextrans; D004964:Estriol / Q000097:blood*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D014280:Triglycerides / Q000097:blood
D003911:Dextrans; D014280:Triglycerides; D002606:Charcoal; D004964:Estriol; D006854:Hydrocortisone