Two monoclonal antibodies recognizing determinants on human embryonal carcinoma cells react specifically with the liver isozyme of human alkaline phosphatase.
From a series of hybridomas that produced monoclonal antibodies reactive with the surface of human embryonal carcinoma cells, two that specifically recognized determinants of the liver/bone/kidney isozyme of alkaline phosphatase were isolated. They did not cross-react with the intestinal or placental isozymes. Phylogenetic studies revealed that both antibodies cross-reacted strongly with liver alkaline phosphatase from higher primates, but exhibited marked differences in their respective cross-reactions with liver alkaline phosphatase from other mammalian species.
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D003429:Cross Reactions; D054278:Embryonal Carcinoma Stem Cells; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007527:Isoenzymes / Q000276:immunology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D014411:Neoplastic Stem Cells / Q000276:immunology*; D011323:Primates; D013045:Species Specificity; D013234:Stem Cells / Q000276:immunology*; D013724:Teratoma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000939:Epitopes; D007527:Isoenzymes; D000469:Alkaline Phosphatase