Cell repair after liver injury: an analysis of some metabolic conditions required for the stimulation of RNA synthesis in postischemic liver nuclei.
Nuclei isolated from liver cells recovering from reversible (non-necrogenic) ischemia show an increased RNA synthesis. The postischemic effect is not abolished by previous adrenalectomy and occurs in fasted as well as in fed animals. Treatment with cycloheximide, at a dose that severely inhibits protein synthesis without primary effects on RNA synthesis, suppresses the postischemic stimulation of RNA synthesis even if cycloheximide is administered when stimulation is already well developed. Postischemic liver nuclei respond only weakly to the additional stimulation of RNA synthesis caused by the presence of cytosol and albumin in the incubation medium.
D000315:Adrenalectomy; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D005215:Fasting; D007511:Ischemia / Q000473:pathology*; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008297:Male; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
D012710:Serum Albumin, Bovine; D012313:RNA; D003513:Cycloheximide; D014357:Trypsin