Effects of cysteamine on pro-somatostatin related peptides.
Intracerebroventricular (icv) injection of cysteamine to rats produced a marked depletion of somatostatin-14-like immunoreactivity (LI) in all rat brain regions examined. The somatostatin-28 (SS28)-LI and SS28(1-12)-LI were generally not altered by the cysteamine treatment. Following subcutaneous injection of the drug similar depletions of hypothalamic SS14-LI was observed with no change in SS28-LI nor SS28(1-12)-LI. In vitro cysteamine significantly increased the basal release of SS14-LI and markedly potentiated the evoked release of SS14-LI from hypothalamic slices. At 10 mM cysteamine, enhanced release of SS14-LI from hypothalamic slices was still observed despite a marked depletion of tissue content of SS14-LI.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003543:Cysteamine / Q000008:administration & dosage / Q000494:pharmacology*; D000939:Epitopes / Q000032:analysis; D007276:Injections, Intraventricular; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D011498:Protein Precursors / Q000096:biosynthesis* / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000276:immunology
D000939:Epitopes; D011498:Protein Precursors; D013004:Somatostatin; D003543:Cysteamine; C028456:prosomatostatin