A model for the excision of introns 1 and 2 from adenoviral major late pre-messenger RNAs.
Total steady-state RNA was extracted from nuclei of HeLa cells late after infection with adenovirus serotype 2. Most of the nuclear RNA is transcribed from the major late transcription unit (16.2 to 100.0 map units). To study the cleavage reactions involved in the splicing of leaders 1 and 2, we have used the S1 nuclease mapping technique with restriction fragments located in the region of intron 1 as DNA probes. The S1 mapping data showed that in total nuclear RNA, RNA species accumulate from which the 5' part of intron 1 has been excised, but which still contain the 3' part of the intron. This indicates that intron 1 can be removed in a stepwise fashion following the 5' to 3' direction. We have compared the nucleotide sequences from the ends of the putative processing intermediates. The internal cleavage sites do not resemble the consensus 5' or 3' splice site sequences. However, they show considerable homology to the sequence 5' A-T-G-A-T-G-G-C-A-T 3', which may act as a signal for internal cleavage. The intermediates are present in both the poly(A)+ and poly(A)- RNA fractions, although with different relative intensities. Primer extension experiments have been performed in which a primer, located with its left end in leader 2, is extended into intron 1. The results show that there may be a cleavage site as short as 35 nucleotides before the 3' splice site. Cleavage at the 3' splice site seems to be rapidly followed by ligation of leader 1 to leader 2. A model for RNA splicing based on these findings and data from the literature is presented.
D000260:Adenoviruses, Human / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D006367:HeLa Cells; D006801:Humans; D008957:Models, Genetic; D009694:Nucleic Acid Precursors / Q000235:genetics*; D011061:Poly A; D012313:RNA / Q000235:genetics; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012342:RNA, Small Nuclear; D012367:RNA, Viral / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D012367:RNA, Viral; D011061:Poly A; D012313:RNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases