Cell-free synthesis of factor VIII related protein.
Polyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunoprecipitation, the translation product was found to contain 1.58% VIII related protein (VIII R), which has a molecular weight of 0.26 X 10(6), similar to the subunit of VIII R present in normal plasma. This indicated that VIII R messenger RNA is responsible for the synthesis of subunits only and intracellular post-translational events are required for the assembly of tetramer and multimers.
D000941:Antigens / Q000096:biosynthesis* / Q000235:genetics; D001345:Autoradiography; D002474:Cell-Free System; D004587:Electrophoresis, Agar Gel; D005169:Factor VIII / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D011061:Poly A / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000302:isolation & purification*; D014841:von Willebrand Factor
D000941:Antigens; D012333:RNA, Messenger; D014841:von Willebrand Factor; D011061:Poly A; D012313:RNA; D005169:Factor VIII