Sensitivity of picroindigocarmine/nuclear fast red (PIC/NF) stain for detection of spermatozoa: a serial dilution study of human ejaculate.
A serial dilution study from human ejaculate was done in order to assess the sensitivity of a commonly employed stain, picroindigocarmine/nuclear fast red, for the detection of spermatozoa. The results of the study indicate that spermatozoa may be detected readily at very low concentrations by scanning 20 high-power fields of a stained smear.
D005260:Female; D005554:Forensic Medicine; D006801:Humans; D007203:Indigo Carmine; D007211:Indoles; D008297:Male; D009499:Neutral Red; D010619:Phenazines; D011902:Rape; D012661:Semen / Q000166:cytology; D013094:Spermatozoa; D013194:Staining and Labeling
D007211:Indoles; D010619:Phenazines; D009499:Neutral Red; D007203:Indigo Carmine