title: Increased expression of beta 2-microglobulin and histocompatibility antigens on human lymphoid cells induced by interferon.
abstract: Normal human peripheral blood lymphocytes were incubated in the presence of different concentrations of interferon for various incubation periods. Subsequently, the amount of beta 2-Microglobulin and HLA-A, B and C surface antigens was estimated by means of quantitative immunofluorescence (flow cytofluorometry) and by a radioimmunoassay for beta 2-Microglobulin. It was found that the amounts of these MHC antigens increased in a dose and time-dependent way after interferon treatment. Furthermore, the influence of different temperatures on this IFN-induced increase in beta 2-Microglobulin was gradually enhanced after incubation at 37 degrees C to 39 degrees C incubation mostly suppressed the beta 2-Microglobulin increase observed at 39 degrees C. The total amount of membrane associated beta 2-Microglobulin was estimated by a radioimmunoassay. After interferon treatment a beta 2-Microglobulin increase was observed ranging from 39% to 122% in different experiments. It is shown that interferon is the substance responsible for the effects on lymphocytes reported here, because completely pure interferon-proteins had the same activity as has been shown for partially purified interferon preparations.
mesh_terms: D001609:Beta-Globulins / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006649:Histocompatibility Antigens; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013696:Temperature; D001613:beta 2-Microglobulin / Q000096:biosynthesis*
chemical_list: D001609:Beta-Globulins; D006649:Histocompatibility Antigens; D001613:beta 2-Microglobulin; D007372:Interferons