Analysis of yeast RNA polymerases with subunit-specific antibodies.
Specific antibodies directed against each polypeptide component of yeast RNA polymerases A or B were prepared and their affinity spectrum determined by protein blot immunodetection. The majority of enzyme A or B subunits were specifically recognized by their respective antiserum. A direct correspondence was established between the polypeptides immunologically related in the three forms of RNA polymerases A, B, and C by reacting the different antibodies with enzymes subunits transferred to a nitrocellulose membrane. Subunit-specific antibodies and antibodies to native enzymes A and B were used to probe the activity of RNA polymerases A, B, and C. Based on DNA protection experiments, the largest subunit of enzymes A and B as well as the common subunit ABC23 appear to be involved in DNA binding.
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D012321:DNA-Directed RNA Polymerases / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes / Q000032:analysis*; D007118:Immunoassay; D007700:Kinetics; D046911:Macromolecular Substances; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases