Protein-membrane interactions: specific vs. non-specific adsorption and binding of proteins and polyamino acids on erythroblasts transformed by Friend virus.
Friend-virus transformed erythroblasts (HFL cells) were incubated in solutions containing up to 3 different proteinaceous compounds at pH 7.2 or 5.5 at 5 degrees C. The specificity of interaction of each compound with the cell surface was determined by comparing the amounts of each compound adsorbed and bound in the presence of 2 or 3 different compounds or after prior binding of another compound to the amounts when the individual compounds alone interacted with the cells. At pH 7.2, ovalbumin and gelatin apparently interacted with cell surface components common to both proteins, as indicated by a decrease (up to 80%) in the amount of each compound adsorbed and bound in the presence, or after the binding, of the other compound. The relative amounts of each compound that interacted were different at pH 5.5 and pH 7.2. Gelatin and poly-L-lysine interacted mainly with different components at pH 7.2, whereas common components appeared to be involved at pH 5.5. Concanavalin A interacted preferentially with components that it shared with lysozyme at both pH values. In addition to variations in interactions with changes in pH and type of compound, variations occurred with changes in concentration of the compounds and with their sequence of addition to the cells. Comparative studies with horse red blood cells showed that the interactions differed markedly with cell type.
D000327:Adsorption; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003208:Concanavalin A / Q000378:metabolism; D004900:Erythroblasts / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005622:Friend murine leukemia virus / Q000502:physiology; D005780:Gelatin / Q000378:metabolism*; D006736:Horses / Q000097:blood; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011107:Polylysine / Q000378:metabolism*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000378:metabolism
D010455:Peptides; D003208:Concanavalin A; D011107:Polylysine; C015475:polysarcosine; D005780:Gelatin; D010047:Ovalbumin; D009113:Muramidase; D012521:Sarcosine