The excretion of human urinary kallikrein quantity and activity in normal and low renin subgroups of essential hypertension.
In both low and normal renin essential hypertensive groups, urinary excretion of kallikrein quantity by direct radioimmunoassay and activity by kininogenase assay were significantly lower than those in normal subjects. In comparing between normal and low renin groups, no difference was found in kallikrein quantity, while kallikrein activity tended to be lower in the low renin group than in the normal renin group. A significant positive correlation was observed between kallikrein quantity and activity in the normal subjects, the normal renin group and the low renin group. However, the slope of the regression line in the low renin group was significantly more moderate than that in the normal renin group, and tended to be more moderate than that in normal subjects. The addition of kallikrein inhibitors (aprotinin and gabexate mesilate) resulted in a significant suppression of enzymatic activity but not of enzyme quantity. These findings suggest that suppression of the renal kallikrein system in both groups of essential hypertension was confirmed by the decreased excretion of kallikrein both as enzyme quantity and activity, and that in the mechanism of the suppression of urinary kallikrein activity in the low renin group, the renal kallikrein inhibitors may play some role.
D000328:Adult; D000368:Aged; D007611:Aprotinin / Q000494:pharmacology; D004796:Clinical Enzyme Tests; D005260:Female; D016670:Gabexate; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D006973:Hypertension / Q000201:enzymology* / Q000652:urine; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000652:urine*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012083:Renin / Q000097:blood*
D006146:Guanidines; D016670:Gabexate; D007611:Aprotinin; D007610:Kallikreins; D012083:Renin