title: Composition and subcellular distribution of glycoproteins and glycosaminoglycans undergoing axonal transport in garfish olfactory nerves.
abstract: The study examined the subcellular distribution of [3H]glucosamine-labeled glycoconjugates undergoing axonal transport in 100,000 x g soluble and two membranous subfractions of the garfish olfactory nerve. Analysis was made of intact glycoconjugates and of glycopeptides and glycosaminoglycans derived from these molecules by limit protease digestion. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed labeling of a variety of high-molecular-weight molecules with a lower molecular weight distribution in the soluble fraction than in the membranous fractions. Following protease digestion, nearly two-thirds of transported radioactivity in glycopeptides was recovered in the plasma membrane-enriched subfraction, with the remainder equally divided between soluble and higher density membrane fraction. Comparison of the distribution of glycopeptide radioactivity and chemically assayed hexosamine revealed transport labeling of a large variety of different-sized neutral and acidic glycopeptides in all subfractions. Transport labeling of most glycoprotein carbohydrate chains was in proportion of their hexosamine content. Transported glycosaminoglycan label was most heavily concentrated in the plasma membrane fraction, whereas hexosamine was most concentrated in the higher density membrane fraction. The labeling pattern suggested both transported and nontransported pools of these molecules. The specific glycosaminoglycans chondroitin sulfate and heparan sulfate were recovered in all subfractions, whereas hyaluronic acid was confined to the soluble fraction.
mesh_terms: D000818:Animals; D001370:Axonal Transport; D002462:Cell Membrane / Q000032:analysis; D002809:Chondroitin Sulfates / Q000032:analysis; D005399:Fishes / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006497:Heparan Sulfate / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D008970:Molecular Weight; D009832:Olfactory Nerve / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis; D014018:Tissue Distribution
chemical_list: D002809:Chondroitin Sulfates; D006023:Glycoproteins; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines; D006820:Hyaluronic Acid