HeLa cell messenger RNAs of widely differing sizes encode certain heat shock proteins in the Mr region of 72 000-74 000.
Cloned cDNA sequences coding for specific 72 000-74 000 Mr HeLa cell heat shock proteins (HSPs) have been used as probes to examine cytoplasmic and nuclear RNA of heat shocked HeLa cells. The mRNAs for these HSPs vary widely in size. Whilst the beta-, delta- and epsilon-HSPs are encoded in mRNAs of 1.9 kb, the gamma-HSP arises from an mRNA three times that size. A search for putative nuclear precursors to these mRNAs revealed high Mr candidates at 15.8 kb which accumulate 1-2 h after the initial heat shock, thus supporting the notion that transcriptional control is important in the initial heat shock response.
D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis; D006367:HeLa Cells / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA