A microplate enzyme-linked immunospecific assay (ELISA) detecting unbound anti-platelet antibodies.
A microplate immune-enzymatic method was developed for detecting serum antiplatelet antibodies. The method involves the use of antigen-coated platelets and alkaline phosphatase-labeled antihuman immunoglobulin. Linear correlation was obtained between the titer of platelet antibodies and substrate conversion. Twenty-four patients with immune thrombocytopenia and 40 normal controls were studied. Eighteen patients and one control were positive. Therefore, sensitivity and specificity were 75% and 97% respectively. ELISA also was found to be more sensitive than the indirect antiglobulin consumption assay (ACA) and appears to be a practical and easy method for routine evaluation of antiplatelet antibodies.
D001666:Binding Sites, Antibody; D001792:Blood Platelets / Q000276:immunology* / Q000378:metabolism; D003298:Coombs Test; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007518:Isoantibodies / Q000032:analysis*; D011696:Purpura, Thrombocytopenic / Q000097:blood / Q000276:immunology
D007518:Isoantibodies