Detection of single antibody-secreting cells generated after in vitro antigen-induced stimulation of human peripheral blood lymphocytes.
Culture and assay procedures are described for the generation and the subsequent detection of single antigen-specific antibody-secreting cells after in vitro stimulation of human peripheral blood lymphocytes with tetanus toxoid. Simple, specific and sensitive, this new assay-culture system is well suited for the analysis of specific antibody production in man.
D000328:Adult; D000921:Antibody-Producing Cells / Q000166:cytology*; D002478:Cells, Cultured; D003584:Cytological Techniques; D000939:Epitopes; D006801:Humans; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D008875:Middle Aged; D013745:Tetanus Toxoid / Q000494:pharmacology*
D000939:Epitopes; D013745:Tetanus Toxoid