A rapid method for preparing undecalcified sections of bone for autoradiographic investigation with short-lived radionuclides.
To prepare sections of undecalcified bone suitable both for autoradiography with short-lived radionuclides such as 99m-technetium (t 1/2 = 6 hr) and for normal histology, rapid processing is necessary. By modifying the routine technique of embedding in plastic, sections can be obtained within 6 hours. The most important modification concerns the temperature used for the different steps in the process. The procedure has been used to localize 99mTc labeled methylene diphosphonate for skeletal scintigraphy.
D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000196:embryology; D005260:Female; D005333:Fetus / Q000000981:diagnostic imaging; D005404:Fixatives; D006801:Humans; D011247:Pregnancy; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013667:Technetium
D005404:Fixatives; D013667:Technetium