title: Plasma protein changes in primary hypertension in humans and rats.
abstract: To determine whether plasma protein changes may be associated with primary hypertension, we analyzed plasma proteins from essential hypertensive (EHT) patients and genetically hypertensive rats using two-dimensional electrophoresis. An additional plasma protein, having a molecular weight of 13,000 daltons and an isoelectric point of 4.5, was found in 82% of the patients with borderline or moderate hypertension (n = 29) and in all permanently hypertensive patients (n = 12). This protein was detected in 36% of normotensive (NT) subjects (n = 50). In the latter, the influence of family history, sex, and secondary hypertension were studied. Plasma proteins were also studied in spontaneously hypertensive rats (SHR). In all plasma from young male (n = 10) and female (n = 6) SHR, two additional proteins (molecular weight = 16,000 daltons, pHi = 4.7 and 5.1) were detectable. These plasma proteins were not detectable in male Wistar Kyoto rats (WKY) and in 50% of female WKY, and their frequency was 10% (n = 10) and 0% (n = 3) in normal male WKY and in male WKY rendered hypertensive by methylprednisolone, respectively. We conclude that these alterations of plasma proteins may be considered a biochemical feature of primary hypertension.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D006973:Hypertension / Q000097:blood*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007676:Kidney Failure, Chronic / Q000097:blood; D008074:Lipoproteins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
chemical_list: D001798:Blood Proteins; C036843:hypertension associated protein, human; C483801:HAP protein, rat; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008074:Lipoproteins; D012709:Serum Albumin; D000511:alpha-Macroglobulins; D005340:Fibrinogen