Characterization of an ATPase on the inside of rat-liver nuclear envelopes by affinity labeling.
Nuclear envelope membranes from rat liver cells contain ATPases, one of which can be inhibited and irreversibly labeled by (S-dinitrophenyl)-6-mercaptopurine riboside triphosphate. Inhibition and covalent substitution of the ATPase are achieved only after disruption of the nuclei, the ATP analogue is inactive on the ATPase activity of whole nuclei or on vesicles of the membrane prepared after a modified heparin method of Bornens and Courvalin. Electron micrographs and scanning micrographs helped to establish the characterization of closed vesicles and intact nuclei. With the aid of (alpha-32P)-labeled, and of the (beta, gamma-32P)-labeled analogue, it was possible to demonstrate the incorporation of the nucleotide into a few protein regions of the nuclear membrane disc electrophoresis pattern.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis; D000345:Affinity Labels / Q000138:chemical synthesis; D000818:Animals; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000201:enzymology; D010539:Permeability; D012313:RNA / Q000378:metabolism; D051381:Rats; D013872:Thionucleosides
D000345:Affinity Labels; D013872:Thionucleosides; C036242:(S-dinitrophenyl)-6-mercaptopurine riboside triphosphate; D012313:RNA; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases