Selective reduction of forskolin-stimulated cyclic AMP accumulation by inhibitors of protein synthesis.
Inhibiting protein synthesis by incubating C6-2B rat astrocytoma cells with cycloheximide or emetine for periods up to 24 hours caused a progressive decrease in the accumulation of adenosine 3',5'-monophosphate (cyclic AMP) when the cells were challenged for 30 minutes with 100 microM forskolin. In contrast, cholera toxin-stimulated (6 nM, 3 hours) cyclic AMP accumulation was not diminished in cycloheximide-treated cells, and cyclic AMP was only minimally diminished in response to a 30-minute challenge with 10 microM (-)-isoproterenol. These experiments suggest the presence of a previously unrecognized cyclase component, which is essential for forskolin-stimulated cyclic AMP accumulation and has a shorter half-life than the beta-adrenergic receptor, the guanine nucleotide regulatory proteins, or the cyclase catalytic component.
D000818:Animals; D001254:Astrocytoma / Q000378:metabolism; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D005576:Colforsin; D000242:Cyclic AMP / Q000096:biosynthesis* / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D004640:Emetine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats
D004224:Diterpenes; D005576:Colforsin; D004004:Dichlororibofuranosylbenzimidazole; D012313:RNA; D002772:Cholera Toxin; D003513:Cycloheximide; D000242:Cyclic AMP; D007545:Isoproterenol; D004640:Emetine