In vitro synthesis of biologically active human leukocyte interferon in a RNA-dependent system from Saccharomyces cerevisiae.
RNA isolated from yeast Saccharomyces cerevisiae containing recombinant plasmid DNA coding for a human leukocyte interferon gene was used as template in a modified yeast cell-free translation system. Radioactive as well as biologically active leukocyte interferon was synthesized.
D002474:Cell-Free System; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013698:Templates, Genetic
D007370:Interferon Type I; D012313:RNA