Deoxyoligonucleotide chain cleavage reactions by phenanthroline and bleomycin.
To elucidate the mechanisms of double-stranded DNA chain cleavage by 1,10-phenanthroline-Cu(I) and bleomycin-Fe(II) complexes in oxygen-dependent reactions, self-complementary deoxyoligonucleotides were treated with the complexes and the degradation products were characterized. The degradation products of d(C-G) by the phenanthroline complex were fully identified by UV, proton and carbon-13 NMR and paper electrophoresis. It is assumed that the DNA chain cleavage and concomitant base release are caused by oxidation of Cl' of the deoxyribose moiety. A duplex of d(ATACGCGTAT) was cleaved by both complexes. Bleomycin shows some sequence specificity but phenanthroline does not.
D001483:Base Sequence; D001761:Bleomycin; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D007501:Iron; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010618:Phenanthrolines; D013329:Structure-Activity Relationship
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010618:Phenanthrolines; D001761:Bleomycin; D003300:Copper; D007501:Iron; C025205:1,10-phenanthroline