Metabolism of purine nucleosides and phosphoribosylpyrophosphate in thymocytes and splenocytes of various mammalian species.
1. Activities of ADA, PNP and AK were measured in splenocytes and thymocytes of newborn children, young horses, pigs, sheep, rats and mice and compared with the activities previously found in peripheral lymphocytes. 2. With all species, except horse, the activity of ADA (per 10(6) cells) was higher in thymocytes than in lymphocytes. Activity of ADA was highest in splenocytes of pig and sheep. Activity of ADA was lowest in all lymphoid cells of the horse and only about 10% of the activity in human splenocytes and lymphocytes. 3. With all species, except horse, the activity of PNP was lower in thymocytes than in lymphocytes. Activity of PNP was highest in human lymphocytes and lowest in ovine thymocytes. 4. Activity of AK is comparable in thymocytes of all species and always lower than the ADA activity. In splenocytes of man, horse and pig the activity of AK is comparable to that in thymocytes. 5. Activity of deoxyguanosine kinase was lowest in rat thymocytes and highest in those of man. 6. When enzyme activities are expressed per milligram of protein, the differences between thymocytes and lymphocytes are less pronounced. 7. Activity of PRPP synthetase per 10(6) cells was comparable in thymocytes, splenocytes and lymphocytes of the same species and between the various species. 8. The concentration of PRPP was lowest in ovine thymocytes and higher in splenocytes than in thymocytes of the same species, except man.
D000243:Adenosine Deaminase / Q000378:metabolism; D000248:Adenosine Kinase / Q000378:metabolism; D000328:Adult; D000818:Animals; D006736:Horses; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D010428:Pentosephosphates / Q000378:metabolism*; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism*; D011684:Purine Nucleosides / Q000378:metabolism*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012268:Ribose-Phosphate Pyrophosphokinase / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology*; D013552:Swine; D013950:Thymus Gland / Q000201:enzymology*
D010428:Pentosephosphates; D011684:Purine Nucleosides; D010754:Phosphoribosyl Pyrophosphate; D011683:Purine-Nucleoside Phosphorylase; D000248:Adenosine Kinase; D012268:Ribose-Phosphate Pyrophosphokinase; D000243:Adenosine Deaminase