In vitro histamine release from human basophils triggered by a purified allergen from Dermatophagoides farinae: bimodal aspect of the dose response curve.
Histamine release experiments were performed over a 10(6)-fold range of allergen concentrations on basophils of patients sensitive to Dermatophagoides farinae. Two extracts of D. farinae were used, a partially purified one (Df 80d) containing several allergens and a highly purified component (Ag11). This histamine release patterns obtained with the complex allergen mixture Df 80d gave broad histamine release curves presenting an initial increase, followed by a decrease or a plateau, with occasionally a small dip. The Ag11-induced histamine release curves presented two peaks (bimodal pattern), separated by a more or less pronounced inhibition dip. No non-specific histamine releasing activity was observed with the basophils of two controls. The possible theoretical models for the bimodal histamine release patterns are discussed.
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001491:Basophils / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000209:etiology; D007073:Immunoglobulin E / Q000096:biosynthesis; D008925:Mites / Q000276:immunology*; D012882:Skin Tests
D000485:Allergens; D007073:Immunoglobulin E