title: Polypeptide clearing in model membranes: an analysis of the partition of gramicidin a' between cadmium ion induced gel and liquid-crystalline phases in vesicles of phosphatidic acid and phosphatidylcholine.
abstract: Using a simple model for a biological membrane we examine cation-induced gel phase formation and the depletion of polypeptide from the gel phase. The model system consists of vesicles of phosphatidic acid and phosphatidylcholine which contain gramicidin A'. By use of electron spin resonance to monitor lipid phase behavior, Cd2+ is found to induce gel and liquid-crystal phase coexistence over a wide range of lipid composition. Quenching of gramicidin A' tryptophanyl fluorescence by spin-labeled phosphatidic acid or spin-labeled phosphatidylcholine is analyzed to obtain the partition coefficient, Kp, for gramicidin A' between gel and liquid-crystal phases. The value of Kp = 3 favoring the liquid-crystal phase indicates a partial clearing of the membrane-bound polypeptide from Cd2+-induced gel phase regions of the membrane.
mesh_terms: D002104:Cadmium / Q000494:pharmacology; D003460:Crystallization; D004578:Electron Spin Resonance Spectroscopy; D006096:Gramicidin; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000502:physiology*; D010712:Phosphatidic Acids / Q000502:physiology; D010713:Phosphatidylcholines / Q000502:physiology; D012995:Solubility; D013050:Spectrometry, Fluorescence
chemical_list: D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D002104:Cadmium; D006096:Gramicidin