[Use of a TLC method for the simultaneous determination of an enzyme complex of nucleic acid metabolism during the isolation of polynucleotide phosphorylase].
For the simultaneous determining of several enzymes of nucleic acid metabolism during polynucleotide phosphorylase isolation TLC was used. It was found that using TLC one can simultaneously detect six and more enzymes, e. g. polynucleotide phosphorylase, 5'-nucleotidase, exoribonuclease together with nucleosidediphosphatase, desaminase etc. The method is simple and accessible.
D015720:5'-Nucleotidase; D002855:Chromatography, Thin Layer / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D009708:Nucleotidases / Q000032:analysis; D011117:Polyribonucleotide Nucleotidyltransferase / Q000302:isolation & purification*; D012313:RNA / Q000378:metabolism*
D012313:RNA; D004247:DNA; D011117:Polyribonucleotide Nucleotidyltransferase; D009708:Nucleotidases; D015720:5'-Nucleotidase
Primenenie metoda TSKh dlia odnovremennogo opredeleniia kompleksa fermentov obmena nukleinovykh kislot pri vydelenii polinukleotidfosforilazy.