Kinetics and drug sensitivity of the anti-hapten and anti-carrier IgG1 and IgG2 antibody production in guinea pigs.
The influence of the hapten-protein ratio on the induction and kinetics of specific IgG1 and IgG2 anti-hapten and anti-carrier antibody synthesis, and the sensitivity of these reactions to cyclophosphamide (CY) and 6-mercaptopurine (6-MP), were studied following immunization with two different dinitrophenylated bovine gamma-globulin (DNP20-BGG and DNP47-BGG) conjugates in Freund's complete adjuvant (FCA) and drug treatment over the first 7 days after antigen injection. The DNP- and BGG-specific IgG1 and IgG2 serum antibody concentrations were determined weekly. Treatment by CY resulted in a complete suppression of the primary IgG1 and IgG2 anti-BGG antibody response, both in the DNP20-BGG and DNP47-BGG immunized guinea pigs. The anti-DNP response was completely suppressed only up to day 14 (DNP20-BGG immunized group) or day 21. This was followed by an increase that was significantly greater in the DNP20-BGG than in the DNP47-BGG immunized animals. The secondary IgG1 anti-BGG immune response induced by an injection of 1 mg BGG on day 86, was uninfluenced in animals immunized with DNP20-BGG but stimulated in the other group treated with DNP47-BGG. The IgG2 was completely suppressed in both groups. 6-MP, known to be less immunosuppressive in guinea pigs, led to very similar results. The finding that in animals immunized with DNP47-BGG the primary anti-BGG IgG2 antibody synthesis was uninfluenced by 6-MP, but the development of a memory was suppressed, would suggest that the primary antibody response and immunological memory are either separate mechanisms or have different sensitivities to 6-MP.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification; D007700:Kinetics; D008297:Male; D015122:Mercaptopurine / Q000494:pharmacology; D005719:gamma-Globulins / Q000276:immunology
D002352:Carrier Proteins; D006241:Haptens; D007074:Immunoglobulin G; C030730:dinitrophenyl gamma globulin; D005719:gamma-Globulins; D003520:Cyclophosphamide; D015122:Mercaptopurine