title: Studies of histamine in the rheumatoid joint.
abstract: Fragments of synovial membranes from three patients with rheumatoid arthritis were incubated in Dulbecco's modified Eagle's medium (DMEM). Histamine was released into the medium at rates up to 649.3 ng/g synovium/day. To investigate whether histamine in synovial fluid may have more than a vasoactive role, cells cultured from human trabecular bone adjacent to a rheumatoid joint and human adherent rheumatoid synovial cells were incubated with different concentrations of histamine for 5 min. Histamine increased the intracellular content of cyclic AMP up to three fold in a dose-related manner but had no effect on synovial cells. This offers preliminary evidence that cells grown from human trabecular bone have histamine receptors.
mesh_terms: D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006632:Histamine / Q000378:metabolism* / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007596:Joints / Q000187:drug effects / Q000378:metabolism*; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D006632:Histamine; D000242:Cyclic AMP