Improved record keeping and photography of silver-stained two-dimensional electrophoretograms by way of "XRD images".
Kodak X-Ray Duplication film provides a relatively simple and inexpensive medium for producing full-size positive-image transparencies ("XRD images") from silver-stained two-dimensional polyacrylamide gel electrophoretograms. In these XRD images, the spot or band pattern of a polychromatic silver-stained gel is represented as black against a clear background, regardless of the color of the spot in the gel itself. Thus, XRD images may be overlapped with transillumination for visual gel-to-gel comparisons, may be easily subjected to scanning densitometry, and may have the exposure adjusted to provide the proper background for black-and-white photography. I describe here the details of the XRD method and the design of a new apparatus for producing XRD images in a wet-gel "contact-print" format, which improves the method.
D000653:Amniotic Fluid / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002824:Chorionic Villi / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing; D010781:Photography / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling; D014962:X-Ray Film
D001798:Blood Proteins; D011506:Proteins; D012834:Silver