Synthesis of electrophoretically restricted IgG by cultured rheumatoid synovium.
IgG synthesized by excised rheumatoid (RA) synovia was separated according to isoelectric point using chromatofocusing and isoelectric focusing. Over 30% of the IgG synthesized by 9 of 11 synovia had an isoelectric point greater than 8 (cathodal shift), while 55% of the synovia secreted IgG showing oligoclonal banding by isoelectric focusing. Oligoclonal serum IgG was seen in 17% of RA patients and in only 3% of hospitalized patients without RA. These results emphasize the selective character of the antigenic stimulus of the RA synovial lymphoid infiltrate.
D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002478:Cells, Cultured; D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D043025:Oligoclonal Bands; D013583:Synovial Membrane / Q000276:immunology*
D000939:Epitopes; D007074:Immunoglobulin G; D007136:Immunoglobulins; D043025:Oligoclonal Bands