title: Nucleotide sequence of Dictyostelium small nuclear RNA Dd8 not homologous to any other sequenced small nuclear RNA.
abstract: The cellular slime mold Dictyostelium discoideum, a lower eukaryote, was shown to contain several species of small nuclear RNA (Takeishi, K., and Kaneda, S. (1981) J. Biochem. (Tokyo) 90, 299-308; Wise, J. A., and Weiner, A. M. (1981) J. Biol. Chem. 256, 956-963). One of these RNAs, Dd9 or D2, was sequenced and found to be homologous to mammalian nucleolar U3 RNA. In the present study, the nucleotide sequence of another Dictyostelium small nuclear RNA Dd8 was determined by direct analysis. The sequence is: (formula; see text) Dd8 RNA contains high proportions of A (34%) and U (30%). No modified nucleotide could be detected in the internal region. Computer-assisted analysis of sequence homology indicated that Dd8 RNA is not homologous to any other small nuclear RNA species sequenced so far.
mesh_terms: D001483:Base Sequence; D004023:Dictyostelium / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D012313:RNA / Q000032:analysis*; D012342:RNA, Small Nuclear; D006163:Ribonuclease T1 / Q000378:metabolism
chemical_list: D012342:RNA, Small Nuclear; D012313:RNA; D006163:Ribonuclease T1