Mapping of antigenic determinants of human apolipoprotein B using monoclonal antibodies against low density lipoproteins.
The reactivity of a series of monoclonal antibodies directed against human low density lipoproteins (LDL) has been tested with hepatic and intestinal apolipoprotein B (apo-B) termed B-100 and B-48, respectively (Kane, J. P., Hardman, D. A., and Paulus, H. E. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 2465-2469). Whereas those antibodies that have been previously shown to recognize determinants close to the LDL receptor recognition site reacted only with B-100, two antibodies specific for other regions of apo-B reacted with both B-100 and B-48. Therefore, it is probable that sequence homologies exist between the two proteins and it must be considered that all or parts of the B-48 sequence may be contained within that of B-100. The specificity of the reaction of these antibodies with proteins designated B-74 and B-26 supports the concept that they represent complementary fragments of B-100. The present results have been incorporated in a theoretical map of the antigenic determinants recognized by these antibodies on the LDL apo-B.
D000818:Animals; D000911:Antibodies, Monoclonal; D001053:Apolipoproteins / Q000097:blood / Q000276:immunology*; D001055:Apolipoproteins B; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008077:Lipoproteins, LDL / Q000097:blood / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D001053:Apolipoproteins; D001055:Apolipoproteins B; D000939:Epitopes; D008077:Lipoproteins, LDL