A rapid method for analysis of the products of in vitro translation of messenger RNA.
A rapid and sensitive method for assaying the production of a specific protein during translation of messenger RNA is described. The method involves running the samples mixed with carrier protein in antibody-containing agarose and determining the radioactivity in the rocket-shaped immunoprecipitates which form. The radioactivity detected is shown to be qualitatively and quantitatively dependent on the amount of immunoreactive protein produced in translation and can thus be used as a measure of specific protein production. The ability of the method to rapidly analyze many samples in parallel is of particular use in screening large numbers of recombinant clones for the ability to bind a specific messenger RNA and affect its translation in vitro.
D000418:Albumins / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D002352:Carrier Proteins; D005786:Gene Expression Regulation; D007122:Immunoelectrophoresis; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis / Q000096:biosynthesis
D000418:Albumins; D002352:Carrier Proteins; D011506:Proteins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins