Isolation of alloreactive CTL clones with cyclical changes in lytic activity.
We have isolated two H-2Dd specific CTL clones from CBA anti-DBA/2 MLC that undergo changes in lytic activity against tumor and Con A blast target cells as a consequence of weekly stimulation with irradiated allogeneic cells and conditioned medium from secondary MLC. Lytic activity is highest on days 2 to 3 after stimulation, and lowest on days 5 to 7. CTL that had been irradiated on day 0 before stimulation have significantly greater activity on day 2. Conditioned media alone, in the absence of added allogeneic stimulator cells is capable of supporting, in a dose-dependent fashion, the increase in activity. Collectively, these changes are similar to those described for the induction of lytic activity in nonlytic CTL populations from long-term MLC, and suggest that these cloned lines may be useful to study the modulation of lytic activity of CTL in culture.
D000818:Animals; D002469:Cell Separation; D002999:Clone Cells / Q000145:classification / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D051381:Rats; D011919:Rats, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors
D000939:Epitopes; D003208:Concanavalin A