[Synthesis and expression of the DNA fragment coding the antigenic determinant of the surface antigen protein of hepatitis B virus].
With the use of chemical-enzymatic synthesis, a polynucleotide (I) has been obtained which codes the amino acid sequence 93-109 of the hepatitis B surface antigen (HBsAg). The plasmid pHS12, constructed by insertion of (I) into pBR325 between EcoRI and BamHI sites, transformed E. coli HB101 cells. The plasmid expression in cell-free system produced a chimeric protein, its molecular weight corresponding to that a polypeptide containing the heptadecapeptide from HBsAg. An approach is discussed for creating a new type of vaccines on the basis of chimeric proteins containing the antigenic determinants of virus proteins.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002678:Chimera; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D000939:Epitopes / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral; D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D014764:Viral Proteins; D004262:DNA Restriction Enzymes
Sintez i ékspressiia fragmenta DNK, kodiruiushchego antigennuiu determinantu belka poverkhnostnogo antigena virusa gepatita B.