Protein adsorbents intended for use in aqueous two-phase systems.
Preparation of adsorbents with high partition coefficients in polyethylene glycol-dextran and polyethylene glycol-phosphate systems is described. These adsorbents may be used to carry to carry proteins away from the insoluble cell fragments generated during cell disruption. By chromatographic elution, proteins may be selectively desorbed in a reduced volume.
D000327:Adsorption; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification; D002458:Cell Fractionation / Q000379:methods; D003911:Dextrans; D005782:Gels; D006593:Hexokinase / Q000302:isolation & purification; D010710:Phosphates; D011092:Polyethylene Glycols; D011188:Potassium; D017680:Potassium Compounds; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012685:Sepharose; D012996:Solutions
D003911:Dextrans; D005782:Gels; D010710:Phosphates; D017680:Potassium Compounds; D011506:Proteins; D012996:Solutions; D011092:Polyethylene Glycols; D012685:Sepharose; C025614:sephadex; C013216:potassium phosphate; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D006593:Hexokinase; D011188:Potassium