Plasma protein kinase activity enhanced by interferon is found in platelets.
A protein kinase activity analogous to that found in interferon-treated HeLa cells is detectable in human plasma rich in platelets. This kinase activity is manifested by the phosphorylation of an endogenous Mr 72000 protein which could be conveniently assayed after partial purification on poly(G)-Sepharose. Here, we show that the protein kinase system in the plasma consists of at least 2 components. The protein kinase is found to be localised in the platelets whereas most of the substrate (the Mr 72000 protein) is found free in the plasma and a fraction of it associated with the surface of platelets.
D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011494:Protein Kinases / Q000097:blood*
D011092:Polyethylene Glycols; D017830:Octoxynol; D007372:Interferons; C010615:Nonidet P-40; D011494:Protein Kinases