Human T cell lines with antigen specificity and helper activity.
Human T blasts, obtained by stimulation of peripheral blood mononuclear cells (PBM) with tetanus toxoid, diphtheria toxoid or Candida albicans, were expanded in long-term culture using alternate periods of antigen restimulation and growth in media containing interleukin 2. The cells gave a proliferative response only to the antigen originally used for stimulation. Such as response was strictly dependent upon the presence of autologous but not of allogeneic mitomycin C-treated mononuclear cells. When added to autologous PBM depleted of E-rosetting cells together with the specific antigen, the T blasts induced a polyclonal proliferation and differentiation of B cells. Allogeneic B cells were activated by antigen-stimulated T blasts only in the presence of irradiated mononuclear cells autologous to the responding T blasts. The above responses seemed not to be regulated solely by the release of soluble factors; apparently cell to cell interactions had to take place to obtain an efficient B cell activation.
D000921:Antibody-Producing Cells / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002460:Cell Line; D000939:Epitopes; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D010950:Plasma Cells / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D013745:Tetanus Toxoid / Q000494:pharmacology
D000954:Antigens, Surface; D000939:Epitopes; D007075:Immunoglobulin M; D013745:Tetanus Toxoid