Modified elastic tissue-Masson trichrome stain.
A combined elastic tissue-Masson technique is presented which stains elastic fibers of all sizes, nuclei and connective tissue. The modified elastic tissue stain consists of hematoxylin, ferric chloride and Verhoeff's iodine; nuclei and elastic fibers are stained blue-black in six minutes without differentiation. By contrast, cytoplasmic elements are stained red, (Biebrich scarlet-acid fuchsin) and collagen is stained green (light green) or blue (aniline blue). The entire staining procedure takes approximately one hour.
D001158:Arteries / Q000166:cytology; D002712:Chlorides; D004547:Elastic Tissue / Q000166:cytology*; D005290:Ferric Compounds; D006416:Hematoxylin; D006801:Humans; D007455:Iodine; D008168:Lung / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*
D002712:Chlorides; D005290:Ferric Compounds; D007455:Iodine; C024555:ferric chloride; D006416:Hematoxylin