Immunochemical cross-reactivity between intact purified myelin basic protein (MBP) and the synthetic encephalitogenic peptide S49.
Three antisera to myelin basic protein--a rabbit antiserum pool against rat myelin, a rabbit antiserum pool against rat myelin basic protein (MBP), and a monkey antiserum against bovine MBP--were found to contain detectable levels of antibodies that would bind radiolabeled S49 (GSLPQKAQRPQDENG). Strongly encephalitogenic in Lewis rat, S49 is a synthetic peptide representing residues 69-84 of bovine MBP with a deletion of glycine-76 and histidine-77 to make it analogous to rat and guinea pig MBPs. The rabbit antimyelin antiserum and the monkey anti-MBP antiserum contained antibodies directed against a non-sequential determinant that required asparagine 84, the glycine-histidine deletion, and residues 69-71 for maximal activity. S49-reactive antibodies from the rabbit anti-MBP antiserum were directed solely against a sequential determinant comprising residues 69-71, S49-reactive antibodies from all three antisera reacted in liquid phase with purified intact rat, guinea pig, and bovine MBP showing that the determinant is exposed for B cell recognition even in bovine MBP and can serve both as immunogen and reactant.
D000595:Amino Acid Sequence; D000818:Animals; D000915:Antibody Affinity; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D008253:Macaca mulatta / Q000276:immunology; D004676:Myelin Basic Protein / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013045:Species Specificity
D000939:Epitopes; D004676:Myelin Basic Protein