A routine method for contrasting elastin at the ultrastructural level.
A reliable, simple, and inexpensive method for ultrastructural investigation of elastin is described. This method uses uranyl acetate dissolved in absolute methanol, followed by an optional lead citrate counterstain. The procedure was tested on a number of animal and human tissues that had been fixed and processed differently.
D000818:Animals; D001011:Aorta / Q000032:analysis; D002356:Cartilage / Q000032:analysis; D002415:Cats; D004549:Elastin / Q000032:analysis*; D005881:Gingiva / Q000032:analysis; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D008643:Mesentery / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D051381:Rats; D012867:Skin / Q000032:analysis; D013194:Staining and Labeling; D014501:Uranium
D009942:Organometallic Compounds; C005460:uranyl acetate; D014501:Uranium; D004549:Elastin