[Methodological studies on the hemolytic anti-bovine gamma globulin plaque technic].
Sheep red blood cells (SRBC) coated with specific bovine antibodies are suitable to detect anti-bovine gammaglobulin (BGG)-antibody-forming cells with the hemolytic plaque technique. The number of the definable anti-BGG-plaque-forming cells is dependent on the antibody density per SRBC. The developed plaques are specific for BGG. They are to inhibit with BGG only not with other proteins. The cellular kinetics of the anti-BGG response is to follow up in the spleen and poplitea lymph nodes of the immunized mice with this modification of the hemolytic plaque technique. The results are discussed.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D012756:Sheep; D005719:gamma-Globulins / Q000276:immunology*
D005719:gamma-Globulins
Methodische Untersuchungen zur hämolytischen Anti-Rindergammaglobulin- (RGG-) Plaquetechnik.