Relationship between cytidine 5'diphosphocholine epitope density on ovalbumin and IgE antibody responses compared to other isotypes.
Ovalbumin was covalently substituted by 6, 18 and 20 residues of Cytidine 5'diphosphocholine per molecule. When injected into BALB/c mice, these products induced distinct immunogenic responses. Without any residue, or with six residues, ovalbumin induced hemagglutinating antibodies (1/256, 21 days after injection) and a response to the passive cutaneous anaphylactic reaction (1/400, 21 days after injection). By contrast, with 18 or 20 residues, ovalbumin brought about the appearance of only a weak titer of hemagglutinating antibodies (1/64, 21 days after injection); no response to the passive cutaneous anaphylactic reaction was observed. No anti-cytidine 5'diphosphocholine antibodies were detected by either reaction. Thus epitope density plays a major role in the IgE immune response to PC-OA.
D000818:Animals; D002794:Choline / Q000031:analogs & derivatives*; D003566:Cytidine Diphosphate Choline / Q000276:immunology*; D000939:Epitopes; D006388:Hemagglutinins / Q000096:biosynthesis; D007073:Immunoglobulin E / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008968:Molecular Conformation; D010047:Ovalbumin / Q000276:immunology*; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D011917:Rats, Inbred Lew
D000939:Epitopes; D006388:Hemagglutinins; D007073:Immunoglobulin E; D003566:Cytidine Diphosphate Choline; D010047:Ovalbumin; D002794:Choline