Gamma-endorphin-like peptides in pituitary tissue: evidence for their existence in vivo.
Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both beta E- and gamma E-like peptides. The gamma E-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic gamma E upon gel permeation chromatography. Immunoreactivity for beta E-like and gamma E-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that gamma E-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.
D000818:Animals; D001923:Brain Chemistry; D004723:Endorphins / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008297:Male; D010902:Pituitary Gland / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin; D018823:gamma-Endorphin
D004723:Endorphins; D001615:beta-Endorphin; D018823:gamma-Endorphin