The serum competitor of oestrogen--rat alpha 1-foetoprotein interactions. Identification as a mixture of non-esterified fatty acids.
The novel endogenous serum ligands of rat alpha 1-foetoprotein previously demonstrated in different mammalian sera were identified by g.l.c.--mass-spectrometric methods as a mixture of non-esterified long-chain and predominantly unsaturated fatty acids. Detailed comparative analyses of these ligands extracted from foetal- and pregnant-rat sera, rat amniotic fluid and foetal human sera are presented. We also show that an important fraction of these ligands remains associated with the rat alpha 1-foetoprotein after purification; analyses are given for the composition of this lipid moiety of the foetoprotein. The physiological relevance of these results is discussed.
D000375:Aging; D000818:Animals; D001667:Binding, Competitive; D004967:Estrogens / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship; D000509:alpha-Fetoproteins / Q000378:metabolism*
D004967:Estrogens; D005230:Fatty Acids, Nonesterified; D000509:alpha-Fetoproteins