The provision of 125I-labelled tracers for radioimmunoassay of haptens: a general approach?
Use of 125iodinated progesterone 11 alpha-glucuronide-tyramine conjugate as radioligand with the majority of antisera raised against progesterone 11 alpha-hemisuccinate-bovine serum albumin is shown to produce steep and sensitive standard curves. Accurate precise and robust assays for progesterone which are clinically useful have been readily developed from such systems. Together with data from other heterologous-bridge systems, the results suggest a hypothesis concerning desirable structural features of the links in heterologous-bridge immunoassays for haptens. The hypothesis should facilitate the establishment of other adequately sensitive external-label assays, especially for gonadal steroids.
D000818:Animals; D002417:Cattle; D000939:Epitopes; D005965:Glucuronates / Q000276:immunology; D006241:Haptens; D006908:Hydroxyprogesterones / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007118:Immunoassay / Q000379:methods; D007457:Iodine Radioisotopes; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology
D000939:Epitopes; D005965:Glucuronates; D006241:Haptens; D006908:Hydroxyprogesterones; D007106:Immune Sera; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine; C030250:progesterone 11-hemisuccinate