Molecular analysis of a mutation conferring the high-lysine phenotype on the grain of barley (Hordeum vulgare).
We have analyzed the molecular nature of the Riso 56 mutation that occurs in barley. This mutation results in a depression of hordein accumulation in the grain and consequently in a higher overall lysine content. In particular, the amount of B hordein, which is encoded by the complex locus Hor-2, is decreased by about 75% because of the absence of the major components. The synthesis of certain minor polypeptides, with properties similar to the major B hordeins, remains unaffected. Analysis of endosperm RNA, by in vitro translation and hybridization to various cloned cDNAs derived from hordein mRNA, shows that mRNA for the major B hordeins is not present in the endosperm. Hybridization of a B hordein cDNA clone to gel-fractionated restriction digests of mutant and wild-type DNA indicates that at least 85 kb of DNA has been deleted from the Hor-2 locus in the high-lysine mutant.
D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D002523:Edible Grain / Q000235:genetics*; D005796:Genes; D005983:Glutens; D001467:Hordeum / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000032:analysis*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D011061:Poly A / Q000032:analysis; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger
D000596:Amino Acids; D010940:Plant Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D005983:Glutens; D008239:Lysine