title: Occupational allergy in horticulture: demonstration of immediate-type allergic reactivity to freesia and paprika plants.
abstract: Patients A and M developed allergic symptoms when working in a greenhouse with paprika plants and freesia plants, respectively. The possible involvement of an IgE-mediated mechanism was investigated with the skin prick test, radioallergosorbent test (RAST) and the histamine release test (HRT). Paprika flower, leaf and stem extract released 58, 47 and 43% of the total amount of histamine from washed leukocytes of patient A. In serum A IgE antibodies against paprika leaves and flowers could be demonstrated by RAST (11% binding of 125I-anti-IgE added). Freesia flower and stem extract released 46 and 43% histamine, respectively, from washed leukocytes of patient M. In the RAST, specific IgE antibodies against freesia flowers and stems were found in serum M (37% binding of 125I-anti-IgE added).
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D000939:Epitopes; D005260:Female; D006636:Histamine Release; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D009784:Occupational Diseases / Q000276:immunology*; D010944:Plants / Q000276:immunology*; D011852:Radioallergosorbent Test; D012882:Skin Tests
chemical_list: D000485:Allergens; D000939:Epitopes; D007073:Immunoglobulin E