Regression of isoproterenol-induced cardiac hypertrophy.
Cardiac hypertrophy was induced in adult female Wistar rats after 8 days of daily subcutaneous injections of isoproterenol (ISO). Regression from hypertrophy was studied following 1, 2, 4, 8, 12, and 20 days of ISO withdrawal. After 8 days of treatment cardiac mass increased 40%. Following ISO withdrawal, ventricular regression occurred during the first 8 days. After 12-20 days of recovery, a new steady-state heart weight to body weight ratio was established that was 12-13% above the controls. The half-time recovery for heart weight was 3.8 days. Ventricular RNA content was stimulated 76% after 8 days of ISO-induced hypertrophy. During regression RNA content decreased rapidly during the first 8 days with a half-time of 3.4 days. Following 20 days of recovery ventricular RNA was still 31% above the controls. However, myocyte RNA was stimulated 86% following 8 days of ISO treatment and returned to control level after 12 days of regression. Myocardial DNA was increased 23% in the hypertrophied hearts and did not change during the recovery period. Hydroxyproline was increased in the ISO-treated hearts and decreased only slightly during the recovery interval. These data indicate that ISO-induced hypertrophy was reversible while ventricular RNA content only partially recovered. Nevertheless, myocyte RNA showed a large stimulation that was completely reversible at least after 12 days of recovery.
D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000032:analysis; D005260:Female; D006909:Hydroxyproline / Q000032:analysis; D007545:Isoproterenol / Q000633:toxicity*; D009206:Myocardium / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D007545:Isoproterenol; D006909:Hydroxyproline