Use of the Bio-Rad silver stain to identify gel purified RNA components of small nuclear ribonucleoprotein antigens.
The ultrasensitive silver staining procedure developed for the detection of proteins in polyacrylamide gels has been used to identify the RNA component of small nuclear ribonucleoprotein antigens recognised by antinuclear antibodies. The technique described is a simple, safe and inexpensive procedure with a sensitivity comparable with that obtained by biosynthetic labelling of RNA with 32P.
D000974:Antibodies, Antinuclear / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001324:Autoantigens; D011232:Chemical Precipitation; D006801:Humans; D007158:Immunologic Techniques; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D012261:Ribonucleoproteins; D012834:Silver; D055517:snRNP Core Proteins; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D000941:Antigens; D001324:Autoantigens; D012313:RNA; D012261:Ribonucleoproteins; D012834:Silver; D055517:snRNP Core Proteins; D000097585:SS-B Antigen