[Effects of poly-L-lysine, poly-L-glycine and poly-L-glutamic acid on ANS fluorescence and electrokinetic potential of splenic lymphocytes in the mouse].
The in vivo effect of either poly-L-Lysine, poly-L-Glutamic acid or poly-L-Glycine on mouse spleen cell populations was investigated. The membrane fluorescence changes of activated splenic lymphocytes were studied. Experiments were performed with the hydrophobic fluorescent probe: 1-anilino-8-naphthalene sulphonate (ANS). Kinetic studies further indicated that the course of fluorescence changes may vary considerably depending upon homopolymers. These fluorescence intensity changes would be in direct or inverse relation to the electrokinetic surface potential changes of activated lymphocytes, as assessed by the electrophoretic mobility analysis. It could be concluded that the relation (direct or inverse) between ANS fluorescence and electrokinetic potential depends upon the net electrical charge of the homopolymer used. In further experiments, the determination of the net electrical charge of some tumour antigens could be considered without hard purifications.
D000200:Action Potentials / Q000187:drug effects; D000817:Anilino Naphthalenesulfonates; D000818:Animals; D005456:Fluorescent Dyes; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides / Q000494:pharmacology*; D011099:Polyglutamic Acid / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology*; D013154:Spleen / Q000502:physiology
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D010455:Peptides; D011107:Polylysine; D011099:Polyglutamic Acid; C011080:polyglycine; C027132:1-anilino-8-naphthalenesulfonate
Effets de la poly-L-lysine, de la poly-L-glycine et de l'acide poly-L-glutamique sur la fluorescence de l'ANS et du potentiel électrocinétique des lymphocytes spléniques de souris.