Efficient extraction of RNA from mammalian tissue.
RNA extraction from mammalian tissue has been compared using the different deproteinizing agents: a) guanidine-HCl, b) guanidinium-thiocyanate, c) buffer-saturated phenol, or d) buffer-saturated phenol followed by a proteinase K digestion of the aqueous phase. Both solid tissues (first, second, and third trimester fetal bovine pancreas), and human white blood cell populations were studied. Degradation, as seen in citric acid-urea agarose gels, and the ability to serve as templates for cell-free protein synthesis were used as criteria to assess the efficiency of the different methods. We conclude that employing buffer-saturated phenol with proteinase K digestion is a superior method for consistent extraction of relatively undegraded RNA in quantitative amounts from mammalian tissue.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004586:Electrophoresis; D006146:Guanidines; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D008722:Methods; D010179:Pancreas / Q000032:analysis; D010636:Phenols; D012313:RNA / Q000302:isolation & purification*
D006146:Guanidines; D010636:Phenols; D012313:RNA