title: Indole metabolism in the pineal organ of the pigeon with special reference to melatonin-synthesizing cells. In vitro study combining thin layer chromatography, autoradiography and pharmacological treatment.
abstract: By means of radioimmunoassay a clear-cut peak of melatonin concentration was found in the pineal organ of the pigeon at the middle of the scotophase (Coisin et al. 1982a). The aim of the present study was to identify the cell type responsible for the nocturnal indole metabolism, including melatonin synthesis, in the pineal of this avian species. After a short-term incubation or organ culture in the presence of [3H]-indolic precursors, [3H]-5-hydroxytryptophan or [3H]-5-hydroxytryptamine, the relative amounts of deaminated and acetylated products occurring in the pineal organ were measured by the use of thin layer chromatography and liquid-scintillation counting. It was possible to modify the relative amounts of deaminated and acetylated indoles by the application of some inhibitors of monoamine oxidase and cyclic nucleotide phosphodiesterase. Irrespective of the experimental conditions, high-resolution autoradiography combined with the above-mentioned radiochemical experiments showed that the cells of the receptor line (modified photoreceptor cells) are responsible for indole storage and metabolism, and very probably also for melatonin biosynthesis. The other cell types of the pineal parenchyma did not display significant labeling.
mesh_terms: D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002855:Chromatography, Thin Layer; D010856:Columbidae / Q000378:metabolism*; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000096:biosynthesis; D007211:Indoles / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D009526:Nialamide / Q000494:pharmacology; D010870:Pineal Gland / Q000166:cytology / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology
chemical_list: D007211:Indoles; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D013806:Theophylline; D006916:5-Hydroxytryptophan; D008550:Melatonin; D009526:Nialamide