Association of human and bovine beta 2-microglobulins with detergent solubilized HLA-A,B antigens.
The association of human and bovine beta 2-microglobulins with detergent solubilized HLA-A,B antigens was analyzed by a direct binding assay using radiolabeled beta 2-microglobulin and an immunoadsorbent containing a monoclonal antibody to the HLA-A,B heavy chains. Binding of beta 2-microglobulin to HLA-A,B heavy chains could be saturated with respect to the amount of membrane glucoprotein in the system and reached steady state after 6 h at 37 degrees C. Inhibition of [125I]beta 2-microglobulin binding to HLA-A,B heavy chains by beta 2-microglobulin purified from human urine or bovine colostrum resulted in identical inhibition curves and apparent dissociation constants of 1 X 10(-8) M. This evidence suggests that beta 2-microglobulins from different species have similar binding sites for HLA-A,B heavy chains.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001609:Beta-Globulins / Q000032:analysis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D006680:HLA Antigens / Q000276:immunology*; D015235:HLA-B Antigens; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D008565:Membrane Proteins / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D001609:Beta-Globulins; D006680:HLA Antigens; D015235:HLA-B Antigens; D007143:Immunoglobulin Heavy Chains; D008565:Membrane Proteins; D001613:beta 2-Microglobulin; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40