A fixative for use in muscle histochemistry.
A fixative solution that preserves the activity of some relevant enzymes in muscle histochemistry is described. Portions of human muscle biopsy specimens and selected murine muscles were fresh frozen or placed in the fixative at room temperature for up to 1 month before freezing. Cryostat sections of fresh frozen and fixed frozen tissue were assayed for nicotinamide adenine dinucleotide phosphate (NADH)-tetrazolium reductase (NADH), several adenosine triphosphatases (ATPases), myoadenylate deaminase (MD), and phosphorylase. NADH, ATPase, and MD activity were preserved following fixation but phosphorylase was not preserved. Murine spleen and kidney were similarly tested for acid phosphatase (acid phos), alkaline phosphatase (alk phos), and nonspecific esterase (NSE). Alk phos activity was preserved but acid phos and NSE activity were significantly reduced following fixation. This fixative is useful in some circumstances for processing or shipping human muscle biopsy specimens and experimental tissues.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D005404:Fixatives; D006651:Histocytochemistry; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009130:Muscle, Smooth / Q000166:cytology; D009132:Muscles / Q000032:analysis* / Q000166:cytology / Q000201:enzymology; D009243:NAD / Q000032:analysis; D013154:Spleen / Q000201:enzymology; D013194:Staining and Labeling
D005404:Fixatives; D009243:NAD; D000469:Alkaline Phosphatase