title: Growth temperature-dependent variation in the bacteriophage-inactivating capacity and antigenicity of Yersinia enterocolitica lipopolysaccharide.
abstract: Growth temperature affected the structure of Yersinia enterocolitica Ye 3827 lipopolysaccharide (LPS). Although Y. enterocolitica Ye 3827 synthesized smooth LPS when grown at a low temperature (25 degrees C), partial smooth-rough transition occurred when the bacteria were grown at the physiological temperature (37 degrees C). The structural alteration was detected by bacteriophage-inactivation assay and chemical and immunological analyses. LPS prepared from bacteria grown at 25 degrees C inactivated a number of bacteriophages that recognize the O-antigenic polysaccharide portion of LPS, whereas more than 3000 times the amount of LPS from bacteria grown at 37 degrees C was required for the same degree of inactivation. The antigenic determinant(s) responsible for the major reaction between 25 degrees C-LPS and anti-25 degree C-bacteria was located on the O-antigenic polysaccharide portion of LPS, but those responsible for the major reaction between 37 degrees C-LPS and anti-37 degrees C-bacteria were located on the R-core or inner portion of LPS.
mesh_terms: D000942:Antigens, Bacterial; D001435:Bacteriophages / Q000254:growth & development*; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes; D006385:Hemagglutination Inhibition Tests; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000276:immunology / Q000502:physiology*; D013696:Temperature; D014775:Virus Activation; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000276:immunology / Q000502:physiology*
chemical_list: D000942:Antigens, Bacterial; D002241:Carbohydrates; D000939:Epitopes; D008070:Lipopolysaccharides