Nerve growth factor increases substance P, cholecystokinin and vasoactive intestinal polypeptide immunoreactivities in primary sensory neurones of newborn rats.
Immunoreactive substance P, somatostatin, gastrin/cholecystokinin and vasoactive intestinal polypeptide were studied in lumbar dorsal root ganglia of 14-day-old rats treated from day 2 to 11 of life with nerve growth factor. Increased staining intensity of neuronal cell bodies and processes for substance P, gastrin/cholecystokinin and vasoactive intestinal polypeptide was observed by immunohistochemistry indicating increased neuronal peptide concentrations. These results were supported by radioimmunoassays showing increased ganglionic levels of substance P and vasoactive intestinal polypeptide. Both techniques, however, failed to show a significant increase of somatostatin levels.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D002766:Cholecystokinin / Q000276:immunology*; D005727:Ganglia, Spinal / Q000276:immunology; D005768:Gastrointestinal Hormones / Q000276:immunology*; D006651:Histocytochemistry; D007158:Immunologic Techniques; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000276:immunology*; D051381:Rats / Q000276:immunology*; D013373:Substance P / Q000276:immunology*; D014660:Vasoactive Intestinal Peptide / Q000276:immunology*
D005768:Gastrointestinal Hormones; D009414:Nerve Growth Factors; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D002766:Cholecystokinin