title: Serotonergic innervation of the rat pituitary intermediate lobe: decrease after stalk section.
abstract: Serotonin (5-HT)-containing nerve fibers and terminals, but not cell bodies, have been demonstrated by light and electron microscopic immunocytochemistry in the intermediate lobe of the rat pituitary. Seven days after pituitary stalk transection, 5-HT immunoreactive fibers disappeared almost entirely from the intermediate lobe. Intermediate lobe 5-HT and 5-hydroxyindoleacetic acid concentrations, measured by high pressure liquid chromatography coupled to electrochemical detection, fell to 56% and 62% of control, respectively. There were no changes in 5-HT concentrations in anterior or posterior lobes of the pituitary or in the median eminence. These findings, and the failure of sympathectomy to cause a drop in pars intermedia 5-HT, indicate that in the intermediate lobe of the pituitary neuronal 5-HT originates in cells situated in the central nervous system.
mesh_terms: D000818:Animals; D004298:Dopamine / Q000032:analysis; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008473:Median Eminence / Q000032:analysis; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000032:analysis / Q000294:innervation* / Q000502:physiology / Q000648:ultrastructure; D010903:Pituitary Gland, Anterior / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis / Q000502:physiology*
chemical_list: D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine