[Difference in the capacity to repair DNA damage in human cells chronically infected with the rubella virus].
Centrifugation of cell lysates in alkaline sucrose gradients and chromatography on hydroxyapatite columns were used to demonstrate inhibition of reparation of mitomycin C-induced DNA damages at the stage of reunification of single-strand breaks of DNA in human HEp-2 cell cultures chronically infected with rubella virus. At the same time, reparation of single-strand breaks of DNA caused by bleomycin occurs with similar intensity both in chronically infected and noninfected HEp-2 cultures. The experimental results suggest that the chronic course of infection in human cells leads to disorders in reparative synthesis of cellular DNA and/or is due to disconnected effect of reparation enzymes in this system.
D001761:Bleomycin / Q000494:pharmacology; D002460:Cell Line; D002908:Chronic Disease; D004260:DNA Repair / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000382:microbiology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D012409:Rubella / Q000378:metabolism*; D014776:Virus Cultivation
D004273:DNA, Neoplasm; D008937:Mitomycins; D001761:Bleomycin; D016685:Mitomycin
Razlichie v sposobnosti k reparatsii povrezhdeniĭ DNK kletok cheloveka, khronicheski infitsirovannykh virusom krasnukhi.