Further studies on bacterial lipopolysaccharide-induced protection against experimental allergic encephalomyelitis in guinea pigs. Effects of chemical modifications.
Bacterial lipopolysaccharide (LPS)-induced protection against experimental allergic encephalomyelitis (EAE) was studied in guinea pigs using chemically modified derivatives. Hydroxylaminolysis or alkaline hydrolysis of LPS, by which ester-linked fatty acids are removed from LPS, resulted in total loss of its mitogenic activity for B lymphocytes, and EAE-suppressive activity was simultaneously reduced. Similar diminished activity was observed in delayed-type skin reactions to myelin basic protein (BP) and anti-BP antibody production detected by passive hemagglutination and enzyme-linked immunosorbent assay (ELISA). These results indicate that the active site of LPS in suppressing EAE is in the lipid portion and that there exists a good correlation between the capacity of LPS to suppress EAE and its mitogenic activity.
D000818:Animals; D000917:Antibody Formation; D055598:Chemical Phenomena; D002621:Chemistry; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000517:prevention & control*; D005260:Female; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology; D004676:Myelin Basic Protein / Q000276:immunology; D012882:Skin Tests; D013154:Spleen / Q000166:cytology
D008070:Lipopolysaccharides; D008934:Mitogens; D004676:Myelin Basic Protein