Analysis of human sweat proteins by two-dimensional electrophoresis and ultrasensitive silver staining.
The polypeptide components of human sweat have been detected with the methylamine-incorporating silver stain (T. Marshall, 1984, Anal. Biochem. 136, 340-346) following high resolution two-dimensional electrophoresis. This technique reveals over 400 polypeptide components in 65 microliters of unconcentrated human sweat.
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling; D013542:Sweat / Q000032:analysis*
D010455:Peptides; D011506:Proteins; D012834:Silver