Secretion of human interferons by yeast.
Plasmids were constructed to direct synthesis of the human interferons IFN-alpha 1, IFN-alpha 2, and IFN-gamma in the yeast Saccharomyces cerevisiae. Expression of IFN genes containing coding sequences for secretion signals resulted in the secretion of IFN activity. A large proportion of the IFN-alpha 1 and IFN-alpha 2 isolated from the yeast cell growth media had the same amino termini as the natural mature interferons, suggesting a removal of the signal sequences identical to that of human cells. These results show that a lower eukaryote, such as yeast, can utilize and process a human signal sequence.
D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D007372:Interferons / Q000235:genetics* / Q000378:metabolism; D010455:Peptides / Q000502:physiology; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D012323:RNA Processing, Post-Transcriptional; D012441:Saccharomyces cerevisiae / Q000235:genetics
D010455:Peptides; D021382:Protein Sorting Signals; D007372:Interferons