Accurate transcription of a cloned gene from Babesia bovis in Saccharomyces cerevisiae.
Two cloned chromosomal segments of the hemoprotozoan Babesa bovis bearing genes from the polymorphic locus designated BabR were inserted into plasmid vector YCp19, cloned in Escherichia coli and then introduced into Saccharomyces cerevisiae. Both genes were transcribed producing poly(A+) RNAs. In at least one case, the transcript was very similar if not identical to that produced in B. bovis. It is therefore possible that S. cerevisiae can recognize at least some hemoprotozoan promoter and poly(A+)-addition signals.
D000818:Animals; D001403:Babesia / Q000235:genetics*; D002874:Chromosome Mapping; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D010957:Plasmids; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA