[Preparation of antibodies against o-aminoazotoluene using chemically and enzymatically synthesized conjugated antigens].
Two methods are developed for synthesis of conjugated antigens to o-aminoazotoluene (o-AAT)-albumin: the enzymatic method--using horseradish peroxidase (HP) and the chemical one--diazomethod. A possible mechanism is suggested for the covalent binding of o-AAT to albumin which is catalyzed by HP. Antibodies to o-AAT are obtained by immunization of animals. An essential difference is established for antigenic o-AAT determinants in conjugates synthesized by the enzymatic and chemical methods.
D000818:Animals; D000906:Antibodies; D000941:Antigens; D001391:Azo Compounds / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes / Q000032:analysis; D006735:Horseradish Peroxidase; D006801:Humans; D007114:Immunization; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D009762:o-Aminoazotoluene / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D001391:Azo Compounds; D000939:Epitopes; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase; D009762:o-Aminoazotoluene
Poluchenie antitel k o-aminoazotoluolu s pomoshch'iu sintezirovannykh khimicheskim i fermentativnym metodami kon''iugirovannykh antigenov.