Proteolytic modifications of the carboxyl-terminal region of H-2Kk.
Conditions were established for the generation of limited proteolysis products from purified H-2Kk in high yield (greater than 70%). Chymotrypsin, trypsin, or papain treatment in buffer containing Nonidet P-40 resulted in removal of discrete segments from the H-2 heavy chain without detectable alteration of the beta 2-microglobulin. The Mr = 47,400 heavy chain was converted to products with Mr = 44,200, 42,800, or 40,600 by treatment with chymotrypsin, trypsin, or papain, respectively. Papain digestion removed both the hydrophilic carboxyl terminus and the hydrophobic regions. The size, detergent binding properties, and products resulting from subsequent papain treatment demonstrated that chymotrypsin or trypsin removed segments of the hydrophilic carboxyl-terminal region of the heavy chain while leaving the hydrophobic (membrane-spanning) and glycosylated NH2-terminal regions intact. Chymotrypsin and trypsin caused rapid and extensive degradation of the H-2Kk heavy chain when treatment was done in buffer containing deoxycholate, suggesting that the protein undergoes partial, but readily reversible, denaturation in this detergent. This may account for the elution of H-2K and D antigens from monoclonal antibody affinity columns by deoxycholate-containing buffers.
D000818:Animals; D000911:Antibodies, Monoclonal; D002918:Chymotrypsin; D003902:Detergents; D000939:Epitopes / Q000032:analysis; D006183:H-2 Antigens; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D010206:Papain; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes; D006183:H-2 Antigens; D046911:Macromolecular Substances; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin; D010206:Papain