[Impulse cytofluorometry of the redox activity of single cells using fluorescent formazan].
The first application of a newly developed fluorescent formazan in flow cytometry is described. The cell surface redox activity of isolated mouse hepatocytes after incubation with the tetrazolium salt forming the new fluorescent formazan and the DNA content after Hoechst staining have been measured simultaneously. 2 parametric distribution patterns have been obtained. This new and sensitive fluorometric technique can be used for automatic measurements of single cells correlating redox activity with other cell parameters.
D000818:Animals; D001391:Azo Compounds; D001562:Benzimidazoles; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D005562:Formazans; D008099:Liver / Q000166:cytology*; D051379:Mice; D010084:Oxidation-Reduction; D010879:Piperazines; D011003:Ploidies; D013194:Staining and Labeling; D013778:Tetrazolium Salts
D001391:Azo Compounds; D001562:Benzimidazoles; D005562:Formazans; D010879:Piperazines; D013778:Tetrazolium Salts; D004247:DNA; C043582:HOE 33378
Impulscytofluorometrie der Redox-Aktivität von Einzelzellen mit einem neuen fluorescierenden Formazan.