Actin polymerization induced by pulsed electric stimulation of bone cells in vitro.
Electric field pulses, capacitively applied to tissue cultures of embryonic bone cells, were shown to induce changes in the state of cellular actin. Three actin states could be defined by DNAase I inhibition. A rapidly (20-30 s) inhibiting fraction, attributed to monomeric G-actin, amounts to 55% of total actin in nonstimulated cells. An additional fraction of 8% required approx. 20 min to reach full inhibition and was tentatively defined as polymeric 'F'-actin. The remaining 37% could be detected only after treatment of the cells with 0.75 M guanidine hydrochloride, which dissociates actin from all its protein interactions. This fraction, N-actin (network actin) is believed to represent F-actin integrated into some supramolecular structure, where it is not accessible to DNAase I. Upon short electric stimulation the distribution changed to 40% G-actin, 12% F-actin and 48% N-actin. 3-Isobutyl-1-methylxanthine (IBMX; an inhibitor of cAMP phosphodiesterase), depletion of extracellular calcium, and calmodulin inhibitors abolished this field effect.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D003850:Deoxyribonuclease I; D004558:Electric Stimulation; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors; D007700:Kinetics; D046911:Macromolecular Substances; D051381:Rats; D013050:Spectrometry, Fluorescence
D000199:Actins; D002147:Calmodulin; D046911:Macromolecular Substances; D000242:Cyclic AMP; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine