The effect of epitope density and antibody affinity on the ELISA as analysed by monoclonal antibodies.
The antibody titre and the optical density values in an ELISA are influenced by the epitope density of the antigen and the affinity of the antibody tested. This has major implications in the interpretation of ELISA results.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000915:Antibody Affinity; D004140:Dinitrophenols / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D007700:Kinetics; D051379:Mice; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; D000939:Epitopes; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine