Generation of antigen-specific suppressor cells in vitro in man.
The generation of human antigen-specific suppressor cells in vitro was examined in a primary anti-DNP antibody-forming system. It was found that specific T8 suppressor cells could be induced in vitro with high doses of antigen. The suppression obtained was macrophage dependent and carrier specific. KLH-specific T8 suppressor cells suppressed the anti-DNP antibody response induced by DNP-KLH but not by DNP-FGG. Similarly, T8 suppressor cells induced with FGG specifically suppress the anti-DNP response stimulated by DNP-FGG. These T8+ suppressor cells bear Ia molecules arising as a consequence of activation. This in vitro antigen-specific system should be useful for the further dissection of human immunoregulatory circuits.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000276:immunology; D002645:Chickens; D004136:Dinitrobenzenes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology*; D006433:Hemocyanins; D006801:Humans; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D004136:Dinitrobenzenes; D000939:Epitopes; D005719:gamma-Globulins; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin