Preparation of an agar-gel-precipitating antigen for avian encephalomyelitis and its use in evaluating the antibody status of poultry.
An agar-gel-precipitin (AGP) antigen for avian encephalomyelitis virus (AEV) was prepared from infected chicken embryo brains. The antigen could precipitate specific antibodies to AEV. No nonspecific reactions were observed. Results of AGP tests were compared with those of virus-neutralization (VN) tests on both unvaccinated and AEV-vaccinated chickens. The AGP test reliably detected antibodies to AEV as early as four days postinoculation. Antibodies persisted in most vaccinated birds for over one year.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens; D001350:Encephalomyelitis Virus, Avian / Q000276:immunology*; D004770:Enterovirus / Q000276:immunology*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000662:veterinary*; D000939:Epitopes; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000175:diagnosis*; D011233:Precipitin Tests / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes