Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein.
Complementary DNA clones that code for the immunogenic region of the Plasmodium knowlesi circumsporozoite protein were shown to contain a tandemly repeating 36-base pair unit. A synthetic dodecapeptide corresponding to the predicted reading frame of the repeating nucleotide unit behaved, in an immunoradiometric assay, identically with the native P. knowlesi circumsporozoite protein. The repeating 36-base pair unit occurred 12 times within the gene and accounts for at least one-third of the amino acid sequence of the surface antigen protein.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000939:Epitopes; D005796:Genes; D009842:Oligopeptides / Q000276:immunology; D010961:Plasmodium / Q000235:genetics / Q000276:immunology*; D011506:Proteins / Q000235:genetics / Q000276:immunology*; D012091:Repetitive Sequences, Nucleic Acid
D000939:Epitopes; D009842:Oligopeptides; D011506:Proteins