In vitro testing for cobalt sensitivity: an aid to diagnosis.
Peripheral blood lymphocytes from 45 patients with a positive patch test to cobalt chloride (CoCl2) and 37 controls were stimulated with various concentrations of CoCl2 and/or cobalt sulphate (CoSO4) or cobalt nitrate (Co(NO3)2) or cobalt acetate [CH3COO)2Co) for various days in culture. Lymphocytes from 35 of these patients showed a significantly greater response than that of the controls. The response occurred in the T enriched population and was monocyte dependent. The strength of the in vivo serial dilution test results did not correlate well with the height of in vitro responses. Lymphocytes from 3 nickel and/or chromium sensitive patients failed to respond to stimulation with cobalt compounds thus confirming the specificity of the reaction. The DNA synthesis test seems to be a reliable in vitro method to aid in the diagnosis of cobalt sensitivity.
D000085:Acetates; D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000175:diagnosis*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008875:Middle Aged
D000085:Acetates; D003035:Cobalt; C025913:cobaltous nitrate; C018021:cobaltous chloride; C026305:cobalt sulfate