Interaction of alpha 2-macroglobulin-bound thrombin with hirudin.
The human thrombin bound to alpha 2-macroglobulin (alpha 2 M) in a 1:1 stoichiometry is still able to interact with one of its specific inhibitors, hirudin. The dissociation constant of the complex hirudin--alpha 2M-bound thrombin is 1 X 10(-7) M, whatever the mode of thrombin binding, covalent or non-covalent.
D002850:Chromatography, Gel; D006629:Hirudins / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D013917:Thrombin / Q000378:metabolism*; D013997:Time Factors; D000511:alpha-Macroglobulins / Q000378:metabolism*
D006629:Hirudins; D046911:Macromolecular Substances; D000511:alpha-Macroglobulins; D013917:Thrombin