[Isolation and in vitro translation of polysomes and the RNA matrix from gibberellic acid-treated etiolated dwarf pea shoots].
The effect of gibberellic acid (GA) on the size of polysomes (PS) and on the specific translation activity of epicotyl PS of two dwarf pea varieties was studied. It was shown that GA does not significantly alter the specific translation activity of PS and of mRNA isolated from dwarf pea epicotyl PS. Electrophoretic separation of the polypeptides synthesized in a cell-free protein-synthesizing system in PS of control and GA-treated shoots revealed no differences between them. Some minor qualitative and quantitative differences in the protein composition of the cytoplasm of control and hormone-treated shoots were found. Possible influence of GA on the composition of a definite class of mRNA and on posttranslational processing of plant proteins is discussed.
D002458:Cell Fractionation; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism; D005875:Gibberellins / Q000494:pharmacology*; D007700:Kinetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000302:isolation & purification; D010944:Plants / Q000378:metabolism*; D010946:Plants, Medicinal; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*; D013698:Templates, Genetic
D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012313:RNA; C007842:gibberellic acid
Vydelenie i transliatsiia in vitro poliribosom i matrichnoĭ RNK, vydelennykh iz obrabotannykh gibberellovoĭ kislotoĭ étiolirovannykh prorostkov karlikovogo gorokha.