In vitro cell perfusion. I. System for continuous observation of cells and analysis of perfusion fluid suitable for isolated mast cell and macrophage studies.
A system is described in which cells are perfused continuously with appropriate medium while simultaneously recording any change in cell morphology together with assay of released products (enzymes and mediators) following immunologic or non-immunologic perturbation. The released products in the perfusate are monitored continuously by automated fluorimetric assay procedures. The system is particularly applicable to investigate or toxicological studies of isolated mast cells or cultured macrophages.
D000818:Animals; D003584:Cytological Techniques / Q000295:instrumentation; D005966:Glucuronidase / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D010477:Perfusion / Q000295:instrumentation*; D011803:Quinine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013997:Time Factors
D011803:Quinine; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase