Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.
Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.
D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics*; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics*; D010982:Platelet-Derived Growth Factor; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D014158:Transcription, Genetic
D009363:Neoplasm Proteins; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA