Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5'-GMP.
RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of RNA.
D005095:Exoribonucleases / Q000494:pharmacology*; D005421:Flavoring Agents; D006150:Guanine Nucleotides / Q000096:biosynthesis*; D006157:Guanosine Monophosphate / Q000096:biosynthesis*; D008837:Micrococcus / Q000201:enzymology*; D012313:RNA / Q000378:metabolism
D005421:Flavoring Agents; D006150:Guanine Nucleotides; D012313:RNA; D006157:Guanosine Monophosphate; D005095:Exoribonucleases; C023871:nuclease H