The efficacy of the immunoperoxidase technique for the detection of several antigens after destaining of stored stained sections.
1. Sections previously stained with hematoxylin-eosin and periodic acid-Schiff were destained and subjected to the peroxidase-antiperoxidase (PAP) technique for the identification of the following antigens: hepatitis B surface antigen, hepatitis B core antigen, alpha-1-antitrypsin, alpha-fetoprotein, immunoglobulin G and immunoglobulin M. 2. The results obtained after destaining of previously stained sections were similar to those obtained with material that had not been stained, indicating that the sensitivity and specificity of the immunological reaction had not been affected by the previous treatments. 3. The results reported here extend the number of antigens that can be detected by the PAP technique after destaining of sections stained by traditional histochemical methods and consequently increase the opportunity for retrospective studies.
D006512:Hepatitis B Core Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D013194:Staining and Labeling; D000515:alpha 1-Antitrypsin / Q000032:analysis*; D000509:alpha-Fetoproteins / Q000032:analysis*
D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins