title: Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes.
abstract: The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.
mesh_terms: D000941:Antigens / Q000032:analysis*; D002843:Chromatin / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D007399:Interphase; D008677:Metaphase
chemical_list: D000941:Antigens; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D000939:Epitopes