Effect of acetoxycycloheximide and dibutyryladenosine cyclic 3':5'-monophosphate on axonal regeneration in the goldfish optic nerve.
Acetoxycycloheximide (AXM) or dibutyryl cyclic AMP (dbcAMP) was injected unilaterally into the vitreous humor of the eye beginning 5-6 days after bilateral optic nerve crush. Injections were repeated every 12-24 h for a total of 3-5 days; goldfish were sacrificed 10 days after lesioning the nerves. At a low dosage of AXM (0.1 microgram daily for 5 days), the mean outgrowth distance in treated neurons was 60% less than in contralateral control neurons. At a high dosage (0.3 microgram daily for 4 days), outgrowth was immediately blocked in both treated and contralateral control axons. Dibutyryl cyclic AMP, in a dose of 5 microM every 12 h for 3 days, produced a 38% reduction in outgrowth distance, associated with a 30% reduction in protein synthesis by the retinal ganglion cells and a 73% reduction in the amount of protein carried by the fast component of axonal transport.
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D001369:Axons / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology*; D003513:Cycloheximide / Q000031:analogs & derivatives* / Q000494:pharmacology; D006054:Goldfish; D009416:Nerve Regeneration / Q000187:drug effects*; D009900:Optic Nerve / Q000187:drug effects*; D012165:Retinal Ganglion Cells / Q000187:drug effects
D003994:Bucladesine; D003513:Cycloheximide; C004434:acetoxycycloheximide