A rapid and reliable in vivo method for anti-GPLA (class I and class II antigens) antibody titration and GPLA typing.
A reverse Arthus reaction (RAR) may be successfully used in guinea pigs to detect histocompatibility alloantigens of the GPLA type. Epidermal cells carry class I GPLA antigens, and Langerhans cells also bear class II alloantigens. It is therefore possible to elicit an RAR by intradermal injection of relevant alloimmune sera in the skin of guinea pigs of known GPLA haplotype. RAR is detected by increased vascular permeability due to IgG1 antibody and hemorrhage due to IgG2 antibody. Compared with an in vitro protein A-rosetting method RAR is easier and quicker. It proved more sensitive for class II antigens in which Langerhans cells are the target for anti-class II antibody and rather less sensitive for class I antigens. RAR is a convenient method for following the course of GPLA alloimmunization, allowing titration of antibodies against both classes of antigen. It may also be used to type guinea pigs of unknown GPLA haplotype.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology / Q000494:pharmacology; D001183:Arthus Reaction / Q000175:diagnosis / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000032:analysis; D006168:Guinea Pigs; D006648:Histocompatibility; D006649:Histocompatibility Antigens / Q000032:analysis* / Q000145:classification; D006650:Histocompatibility Testing / Q000379:methods*; D007518:Isoantibodies / Q000032:analysis*; D012397:Rosette Formation; D012882:Skin Tests
D000961:Antilymphocyte Serum; D000939:Epitopes; D006649:Histocompatibility Antigens; D007518:Isoantibodies