title: Sucrose modifies ribosomal stability and conformation.
abstract: High concentrations of sucrose have a strong protective effect on heat-induced modifications of rat liver ribosomal subunits. They prevent to a large extent subunit inactivation, measured by poly (U)-dependent [14C] Phe tRNA binding (40S subunits) and puromycin reaction (60S subunits), subunit unfolding into light forms, and the release of both free and protein-complexed 5S RNA. They also increase the temperature at which subunits start to melt. Our data indicate that sucrose affects subunit conformation.
mesh_terms: D000818:Animals; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D011072:Poly U / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012342:RNA, Small Nuclear; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000187:drug effects*; D013395:Sucrose / Q000494:pharmacology*
chemical_list: D046911:Macromolecular Substances; D012335:RNA, Ribosomal; D012342:RNA, Small Nuclear; D012346:RNA, Transfer, Amino Acyl; D011072:Poly U; D011691:Puromycin; D013395:Sucrose; D012313:RNA