Identification of an antigenic determinant of mouse lactate dehydrogenase C4.
A peptide bearing an antigenic determinant of the sperm-specific lactate dehydrogenase C4 isozyme (LDH-C4) has been isolated from a tryptic digest of the whole protein. This peptide, comprising residues 152-159 (MC152-159), reacts with rabbit anti-mouse LDH-C4. Immunization of rabbits with synthetic MC152-159 conjugated to bovine serum albumin induces an immune response which is specific for the peptide. Anti-MC152-159 IgG binds 125I-labeled mouse LDH-C4 and competition experiments demonstrate the specificity of this antigen antibody reaction.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002852:Chromatography, Ion Exchange; D000939:Epitopes / Q000032:analysis*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D014357:Trypsin
D000939:Epitopes; D007527:Isoenzymes; D010446:Peptide Fragments; D007770:L-Lactate Dehydrogenase; D014357:Trypsin