Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities.
The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001665:Binding Sites; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D064429:Fatty Acid Synthases / Q000276:immunology; D005493:Folic Acid Antagonists; D005954:Glucosephosphate Dehydrogenase / Q000276:immunology; D009249:NADP / Q000378:metabolism*; D010088:Oxidoreductases / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D000939:Epitopes; D005493:Folic Acid Antagonists; D009249:NADP; D010088:Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases