Flagellar regeneration of the trypanosome Crithidia fasciculata involves post-translational modification of cytoplasmic alpha tubulin.
Deflagellation of Crithidia fasciculata stimulated formation of new flagella and maximized production of alpha 3 tubulin. Continuous labeling during reflagellation revealed that alpha 1, 2, and 3 tubulins were formed, whereas the polyadenylated RNA translation products lacked alpha 3 isoform. Pulse-chase labeling experiments demonstrated that alpha 3 was a post-translational modification of cytoplasmic alpha tubulin.
D000818:Animals; D003421:Crithidia / Q000502:physiology*; D005407:Flagella / Q000502:physiology*; D007700:Kinetics; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012038:Regeneration; D013997:Time Factors; D014404:Tubulin / Q000235:genetics*
D012333:RNA, Messenger; D014404:Tubulin; D011061:Poly A; D012313:RNA