Human liver RNA-programmed in vitro synthesis of a polypeptide related to human apolipoprotein B.
In an in vitro synthesizing system programmed with RNA from human liver a polypeptide with an estimated Mr of 80 000 (80 kDa) +/- 1400 (mean +/- SD, n = 5) was synthesized. This polypeptide could be precipitated with antiserum to a narrow density cut of LDL (d = 1.030-1.055) or antiserum against the high-Mr form of apoB (apoB 100 [4]). The synthesized protein is immunologically related to a 75 kDa protein isolated from LDL. We suggest that the 80 kDa protein represents a primary translation product of apoB synthesized in human liver.
D000818:Animals; D001053:Apolipoproteins / Q000276:immunology*; D001055:Apolipoproteins B; D002474:Cell-Free System; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007163:Immunosorbent Techniques; D008077:Lipoproteins, LDL / Q000276:immunology; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000276:immunology; D012313:RNA / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D001053:Apolipoproteins; D001055:Apolipoproteins B; D000939:Epitopes; D008077:Lipoproteins, LDL; D010455:Peptides; D012313:RNA