Replacement of sodium chloride with dextran or polyethylene glycol for immunoprecipitation of lipopolysaccharide with antibodies in chicken or turkey sera.
Dextran or polyethylene glycol could replace sodium chloride in agarose gels for inducing immunoprecipitation of Pasteurella multocida lipopolysaccharides with antibodies in chicken or turkey sera. Resolution of immunoprecipitates was best when 3% concentrations of either dextran or polyethylene glycol were used. Higher concentrations increased opacity of the gels. Nonspecific precipitation of serum or gamma-globulin fractions in gels was caused by the electrophoresis buffer, dextran, and polyethylene glycol. Dialysis of serum or gamma-globulin fractions against the electrophoresis buffer and soaking gels in buffers of pH greater than 7.0 that contained 3% polyethylene glycol reduced nonspecific precipitation. Incorporation of dextran or polyethylene glycol into gels enhanced immunoprecipitation in rocket immunoelectrophoresis but resulted in slower mobility of antigen.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D002645:Chickens / Q000276:immunology*; D003911:Dextrans; D005260:Female; D007122:Immunoelectrophoresis; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D010325:Pasteurella / Q000276:immunology; D011092:Polyethylene Glycols; D011233:Precipitin Tests / Q000379:methods / Q000662:veterinary*; D012965:Sodium Chloride; D014422:Turkeys / Q000276:immunology*
D000907:Antibodies, Bacterial; D003911:Dextrans; D008070:Lipopolysaccharides; D011092:Polyethylene Glycols; D012965:Sodium Chloride