Common features of the yes and src gene products defined by peptide-specific antibodies.
Anti-peptide antibodies generated against a hydrophilic domain of pp60src comprising amino acid residues 498 through 512 were shown to be cross-reactive with the corresponding region in the yes transforming proteins encoded by Yamaguchi 73 and Esh sarcoma viruses. This cross-reactivity was demonstrated by immunoblot and immunoprecipitation analyses, and the identity of the proteins was verified by partial proteolytic mapping. By utilizing a combination of immunofluorescence and interference-reflection microscopy, these cross-reactive anti-peptide antibodies were shown to produce an immunofluorescence staining pattern in Yamaguchi 73 and Esh sarcoma virus-transformed chicken embryo fibroblasts remarkably similar to that pp60src in Rous sarcoma virus-infected chicken cells. Like the src gene products, the yes transformation-specific polyproteins were found to be concentrated within adhesion plaque structures and needle-like interdigitating cell-cell junctions. This analogous subcellular distribution suggests that these onc proteins are functionally related and may share common intracellular targets.
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002478:Cells, Cultured; D002642:Chick Embryo; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005796:Genes; D005814:Genes, Viral; D015688:Oncogene Protein pp60(v-src); D010446:Peptide Fragments / Q000032:analysis*; D011494:Protein Kinases / Q000032:analysis; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D000914:Antibodies, Viral; D000939:Epitopes; D010446:Peptide Fragments; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)