Effects of synthesis inhibition and nervous activity on concentrations of neuronal substance P.
1. When dorsal root ganglia were incubated in vitro with a range of concentrations of anisomycin, incorporation of [ 3H ]lysine into protein and synthesis of substance P(SP) were inhibited to a similar extent confirming that SP is synthesised by a conventional ribosomal mechanism. 2. Doses of anisomycin sufficient to inhibit protein synthesis in mouse CNS in vivo by greater than 95% did not cause a significant fall in the SP content of any of five brain areas, spinal cord, dorsal root ganglia or cornea over an 8 h period. 3. Electrical stimulation of the hind limbs however, produced a 25% fall in the SP content of the dorsal spinal cord of these animals. 4. These findings suggest that the turnover-time of neuronal SP is much longer than that of non-peptide neurotransmitters.
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D051379:Mice; D009417:Nerve Tissue / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000378:metabolism*; D013373:Substance P / Q000032:analysis / Q000096:biosynthesis*
D009419:Nerve Tissue Proteins; D013373:Substance P; D000841:Anisomycin