Effect of fixation on the ultrastructural localization of delta 53 beta-hydroxysteroid dehydrogenase in adrenocortical and Leydig cells of the rat.
A comparison of the effects of perfusion and immersion fixation on localization of delta 53 beta-HSD in adrenocortical and testicular Leydig cells has been made. The results demonstrated that regardless of the mode of fixation, localization of the enzyme (smooth endoplasmic reticulum) and variability in staining intensity were similar in both tissues. Also noted was the uneven distribution of the staining reaction product within the cells. This finding was interpreted as an expression of regional disparity in metabolic activity rather than artefactual since this phenomenon was noted in both perfusion and immersion fixed tissue.
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000302:Adrenal Cortex / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D005404:Fixatives; D006651:Histocytochemistry; D007985:Leydig Cells / Q000201:enzymology* / Q000648:ultrastructure; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D013194:Staining and Labeling; D013737:Testis / Q000201:enzymology
D005404:Fixatives; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase