Dextran penetration through nonkeratinized and keratinized epithelia in monkeys.
The purpose of this study was to determine if polysaccharide dextrans would pass through intact-nonkeratinized and induced-keratinized sulcular epithelia in monkeys. Dextran penetration through normally keratinized oral gingival epithelium also was evaluated in the same gingival specimens. Each of three Rhesus monkeys received a thorough prophylaxis 1 week prior to the experiment. During this week, the monkeys also received daily IV injections of Achromycin. After the antibiotic treatment, the teeth were polished and cleaned with a rubber cup using prophylactic paste for 2 consecutive months, as follows: (1) the right maxillary and mandibular teeth received daily prophylaxes on weekdays and (2) the left maxillary and mandibular teeth received one prophylaxis weekly. These frequencies of plaque removal on one-half of the mouth maintained clinically healthy gingiva and produced keratinization of the sulcular epithelium. At the end of the 2-month prophylaxes, a 5% solution of dextrans derived from Leuconostoc mesenteroides was applied topically to the gingiva once daily for 3 consecutive weeks. During this time, the monkeys continued having dental prophylaxes following the previous time schedule. The study showed that induced-keratinized sulcular epithelium as well as normally keratinized oral gingival epithelium resisted penetration of dextrans, whereas intact-nonkeratinized sulcular epithelium apparently lacked a surface layer resistant to penetration.
D000287:Administration, Topical; D000818:Animals; D003777:Dental Prophylaxis; D003911:Dextrans / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D004848:Epithelium / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism*; D008253:Macaca mulatta; D008297:Male
D003911:Dextrans; D007633:Keratins