title: Increased in vitro labeling of stable RNA within the rat nodose ganglion following abdominal vagotomy.
abstract: An in vitro procedure for labeling of RNA in the excised rat nodose ganglion was used to evaluate the changes in incorporation of [3H]uridine into ganglionic RNA following transection of the abdominal vagus nerves. Significant increases in the incorporation into 28S, 18S and 4S RNA were observed at 1 day after injury, which were maximal at 4 days before returning to unoperated control level by 7 days. A second transient increase in the labelling of these RNA species occurred between 9 and 11 days after injury. Comparison of the time course of these increases with those seen previously following cervical vagus nerve crush injury indicate that the time of onset of the increase in incorporation in independent of the site of injury, but that the maximal response is delayed by 1 day with the more distal lesion. These data are consistent with the existence of separate signals for initiating and modulating the cell body response to axon injury, which are transported retrogradely from the site of injury at rates exceeding the slow component of axoplasmic transport.
mesh_terms: D000818:Animals; D005260:Female; D007700:Kinetics; D008970:Molecular Weight; D009620:Nodose Ganglion / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D014529:Uridine / Q000378:metabolism; D014628:Vagotomy; D014630:Vagus Nerve / Q000378:metabolism*
chemical_list: D012335:RNA, Ribosomal; D012313:RNA; D014529:Uridine