I. Characterization of cytotoxic effector cells generated from regional lymph nodes after immunization in the footpad.
A system is described which involves an in-vivo and an in-vitro step for the generation of strong primary cytotoxic T lymphocyte (CTL) responses to multiple minor alloantigens. The protocol consists of immunizing mice in the footpad with irradiated, minor alloantigen-different spleen cells. Four days later, cells from the lymph nodes draining the site of injection are cultured for 3 days in the absence of antigen to allow the development of cytotoxic activity. The analysis of the effector phase of the response indicates that the killer cells are H-2 restricted cytotoxic cells specific for minor alloantigens. The in-vitro phase of these cytotoxic responses is T cell-dependent and the responder cells proliferate strongly during the culture period. Both cytotoxic and proliferative responses are abolished by passing responder cells over Sephadex G-10 prior to culture. The results suggest that the maturation process of CTL-precursor (CTL-P) cells into effector CTL in vitro depends upon proliferating T cells and accessory cells.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006614:Hindlimb; D007519:Isoantigens / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000939:Epitopes; D007519:Isoantigens