A new and simple method for studying the microanatomy of nephrons and collecting tubules.
A simple procedure for studying the microanatomy of the kidney by light microscopy is described. Specimens are dried by the critical-point method and later impregnated with a mounting medium. The retention of air within the lumen of nephrons and collecting tubules permits their visualization. The method allows the observation of renal microanatomy without the distortions produced by microdissection or the loss of structural relationships among different kidney components. It also permits observation of the same specimen by both light and scanning electron microscopy.
D000388:Air; D000818:Animals; D003890:Desiccation; D006651:Histocytochemistry / Q000379:methods; D007684:Kidney Tubules / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D009399:Nephrons / Q000648:ultrastructure*; D011817:Rabbits; D013194:Staining and Labeling