Anti-idiotypic regulation of an insulin-reactive T cell clone.
A series of MHC-restricted, bovine-insulin-(BI) reactive T cell clones were generated. The specificity of one group was shown to be for an insulin A-chain loop determinant; the other group apparently demonstrated specificity of a B-chain determinant and/or amino acid residue A4. Guinea pig anti-idiotypic antisera were prepared against two idiotypically related BI monoclonal antibodies of similar A-chain loop specificity. These reagents were able to modulate the antigen-specific proliferation of an insulin-reactive, A-chain loop-specific T cell clone. Because the monoclonal antibodies and the T cell clone recognize a similar molecular domain of the insulin molecule, these data suggest that the anti-idiotypic sera mimic an insulin-like determinant, perhaps by bearing an "internal image" of the antigen and thereby interfering with T cell antigen recognition. Further, these results suggest that such reagents may be useful in characterization of T cell antigen receptor specificity and lend further credence to the concept of idiotypic-anti-idiotypic regulation of the immune response.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D002417:Cattle; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006168:Guinea Pigs; D006183:H-2 Antigens / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007328:Insulin / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006183:H-2 Antigens; D007130:Immunoglobulin Idiotypes; D007328:Insulin