Indiscriminate degradation of RNAs in interferon-treated, vaccinia virus-infected mouse L cells.
In this report we used Northern blot hybridization analysis to characterize the fate of several species of viral RNA transcribed from internal and terminal regions of vaccinia DNA in interferon-treated, infected mouse L cells grown in suspension. All species of viral RNAs were expressed but were reduced in amount. Larger-sized RNAs were reduced more than smaller-sized RNAs. This reduction appears to be related to the activation of the interferon-mediated double-stranded RNA-dependent 2-5A synthetase-endoribonuclease system, as the rRNA cleavage pattern characteristic of this system was observed early in infection and in cell extracts in response to exogenous 2-5A. Thus, in interferon-treated, vaccinia-infected mouse L cells in suspension, there is indiscriminate degradation of viral and cellular RNAs, and this RNA breakdown might play a role in the interferon-mediated inhibition of protein synthesis.
D000818:Animals; D002472:Cell Transformation, Viral; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D014616:Vaccinia virus / Q000187:drug effects / Q000235:genetics*
D012367:RNA, Viral; D012313:RNA; D007372:Interferons