title: Isolation and characterization of two new peptides, mastoparan C and crabrolin, from the venom of the European hornet, Vespa crabro.
abstract: Two peptides rich in hydrophobic amino acids have been isolated from venom sacs of the European hornet, Vespa crabro. One peptide (P-2) is structurally and functionally related to the tetradecapeptide mastoparan and has been named mastoparan C. Leu-Asn-Leu-Lys-Ala-Leu-Leu-Ala-Val-Ala-Lys-Lys-Ile-LeuNH2. The other (P-1) is a tridecapeptide with a new sequence: Phe-Leu-Pro-Leu-Ile-Leu-Arg-Lys-Ile-Val-Thr-Ala-LeuNH2 which we have named crabrolin. The peptide releases histamine from rat peritoneal mast cells with a threshold of approximately 2.5 micrograms/ml (congruent to 8 microM). Crabrolin also facilitates the action of purified phospholipase A2 from different sources, but it is not quite as active as mastoparan. It is clearly less active than mastoparan in lysing erythrocytes, and it does not release amylase from dispersed guinea pig pancreatic acini. Given its unique sequence, the principal effect of crabrolin may be neither mast cell degranulation nor phospholipase facilitation, but a yet undiscovered action.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001514:Bee Venoms / Q000302:isolation & purification*; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D006927:Hymenoptera; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D012911:Snakes; D013552:Swine; D014357:Trypsin; D014862:Wasp Venoms / Q000302:isolation & purification* / Q000633:toxicity; D014863:Wasps
chemical_list: D001514:Bee Venoms; D010446:Peptide Fragments; D014862:Wasp Venoms; C042521:crabrolin; C042520:mastoparan C; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D014357:Trypsin