title: Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence.
abstract: Counterstaining with the chromosomal dye Hoechst 33258 is a simple procedure that provides an excellent general purpose nuclear counterstain for immunofluorescent work. Benefits from its use include the ready identification and orientation of structures, differentiation of lymphoid and non-lymphoid cells and easy assessment of frequency in specifically labelled cellular subpopulations. It can be used at the same time as FITC and TRITC double-labelling immunofluorescence.
mesh_terms: D001562:Benzimidazoles; D006690:Bisbenzimidazole; D002467:Cell Nucleus / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence / Q000379:methods*
chemical_list: D001562:Benzimidazoles; D006690:Bisbenzimidazole