Kinetics of lead citrate staining of thin sections for electron microscopy.
The staining of thin sections with lead citrate shows an initial increase followed by a decrease much later; the rate of the initial increase and subsequent loss varies for different cellular components. The decrease eventually reaches a stable minimum. At this level electron scattering is less than that of unstained sections, demonstrating a loss of biological material. Lead citrate used as a poststain following uranyl acetate causes an increase in electron density that is independent of staining time over 1-30 min; this increase appears to depend only on the quantity of uranyl acetate already bound, implying that the lead binds predominantly to the uranyl acetate.
D000818:Animals; D002645:Chickens; D002951:Citrates; D019343:Citric Acid; D004912:Erythrocytes / Q000648:ultrastructure; D005976:Glutaral; D007700:Kinetics; D008099:Liver / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D012617:Sea Urchins; D013194:Staining and Labeling; D013737:Testis / Q000648:ultrastructure
D002951:Citrates; D019343:Citric Acid; D005976:Glutaral