Characterization of monoclonal antibodies against beta-bungarotoxin and their use as structural probes for related phospholipase A2 enzymes and presynaptic phospholipase neurotoxins.
Hybridoma lines secreting monoclonal antibodies against a phospholipase-inactive derivative of the presynaptic neurotoxin, beta-bungarotoxin, have been established. These antibodies, either of the IgG1 or IgG2b isotype with affinities in the range 1-2 X 10(8) 1/mol, recognized a single immunodominant region of native beta-bungarotoxin, most probably located on the A (phospholipase homologue) chain of the toxin. Using plate-adsorbed radioimmunoassay procedures, antibodies reacted with native beta-bungarotoxin and other beta-bungarotoxin isotoxins as well as with the non-toxic phospholipase A also present in Bungarus multicinctus venom. Other phospholipase A enzymes and presynaptic phospholipase neurotoxins did not show any competition with beta-bungarotoxin in the radioimmunoassay. Globulin fractions of monoclonal antibodies partially inhibited the phospholipase activity of beta-bungarotoxin.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002038:Bungarotoxins / Q000032:analysis / Q000276:immunology* / Q000633:toxicity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas; D051379:Mice; D008815:Mice, Inbred Strains; D010741:Phospholipases A / Q000032:analysis / Q000037:antagonists & inhibitors / Q000276:immunology; D054467:Phospholipases A2; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D002038:Bungarotoxins; D000939:Epitopes; D010741:Phospholipases A; D054467:Phospholipases A2