High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method. Using this procedure, we assigned to specific chromosome sites three cloned genes and one DNA polymorphism: amylase gene (AMY) to 1p21; proopiomelanocortin gene (POMC) to 2p23, somatostatin gene (SST) to 3q28, and a single copy DNA segment (D3S1) to 3q12.
D000681:Amylases / Q000235:genetics*; D002871:Chromosome Banding; D002900:Chromosomes, Human, 1-3; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D010908:Pituitary Hormones, Anterior / Q000235:genetics*; D011333:Pro-Opiomelanocortin; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D013004:Somatostatin / Q000235:genetics*
D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D013004:Somatostatin; D011333:Pro-Opiomelanocortin; D004247:DNA; D000681:Amylases