Determination of alphamethyldopa, dihydroxyphenylacetic acid and 5-hydroxyindolacetic acid in the cerebrospinal fluid of the rat: kinetic study.
Cerebrospinal fluid (CSF) was removed at a constant flow rate of 1 microliter min-1 from the third ventricle of rats and directly analyzed by liquid chromatography coupled with electrochemical detection. The effect of alphamethyldopa (alpha MD) on CSF alpha MD, dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindolacetic acid (5-HIAA) concentrations was studied for 5 h. alpha MD ethyl ester administration resulted in higher alpha MD levels in CSF as compared with alpha MD administration, suggesting a higher bioavailability of the ethyl ester vs the free acid. A specific inhibition of dopamine metabolism was reflected by a reduction in DOPAC levels while 5-HIAA concentrations were unchanged.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid*; D000818:Animals; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D007700:Kinetics; D008297:Male; D008750:Methyldopa / Q000134:cerebrospinal fluid*; D010648:Phenylacetates / Q000134:cerebrospinal fluid*; D051381:Rats; D011919:Rats, Inbred Strains
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D008750:Methyldopa