Evaluation of four reagents for delipidation of serum.
Four reagents, Aerosil 380, Freon 113, Dextran sulfate 500-S, and a mixed organic solvent were tested for their abilities to produce optically clear, pooled human serum. Aerosil-380, a silicon dioxide, removed 95% of serum cholesterol and triglycerides, and 80% of the free fatty acids. A mixed organic solvent (n-butanol:diisopropyl ether) was equally effective, but also removed nearly all endogenous alkaline phosphatase and lactate dehydrogenase. Freon-113 and Dextran sulfate 500-S removed about half of the serum cholesterol and triglycerides. The serum content of several non-lipid components was unaffected by Aerosil-380, Freon-113, and Dextran sulfate treatments; however, the mixed organic solvent removed 69% of the endogenous calcium. Light scattering data revealed that treatment with all reagents except the mixed organic solvent resulted in optically-clear serum products.
D001774:Blood Chemical Analysis; D005617:Chlorofluorocarbons, Methane / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D006801:Humans; D000960:Hypolipidemic Agents; D066298:In Vitro Techniques; D012997:Solvents / Q000494:pharmacology*; D013431:Sulfates / Q000494:pharmacology*
D005617:Chlorofluorocarbons, Methane; D003911:Dextrans; D000960:Hypolipidemic Agents; D012997:Solvents; D013431:Sulfates