[Electron-radioautographic study of the effect of potassium orotate on RNA and protein synthesis in fibroblasts during experimental wound healing].
Electron microscopic radioautography with the use of the double-labeling technique was applied to study potassium orotate influence on RNA and protein synthesis in fibroblasts of intact mouse skin and in granulation tissue on days 3 and 7 of the healing of a musculocutaneous wound. Experimental animals received potassium orotate per os in a daily dose of 0.2 g/kg immediately after operation. Intact animals received the drug for 3 days. According to the assay data potassium orotate did not potentiate the protein-synthesizing activity of an individual cell but apparently activated the proliferation of wound fibroblasts.
D000818:Animals; D001345:Autoradiography; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009963:Orotic Acid / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012867:Skin / Q000187:drug effects / Q000293:injuries*; D014945:Wound Healing / Q000187:drug effects*
D009963:Orotic Acid; D012313:RNA
Elektronno-radioavtograficheskoe izuchenie vliianiia orotata kaliia na sintez RNK i belka v fibroblastakh pri zazhivlenii éksperimental'nykh ran.