Phenylalanyl-tRNA synthetases from yeast cytoplasm and mitochondria. The presence of a carbohydrate moiety in the mitochondrial enzyme and immunological evidence for structural relationship.
Homogeneous yeast cytoplasmic and mitochondrial phenylalanyl-tRNA synthetases (L-phenylalanine:tRNAPhe ligase (AMP-forming), EC 6.1.1.20) are analysed for structural differences. Only the large subunit of the mitochondrial enzyme is a glycoprotein with nearly 3% carbohydrate by weight. The carbohydrates present are: glucose, N-acetylglucosamine, mannose, galactose and N-acetylneuraminic acid. Removal of the sugar moieties yields an activity increase, but no significant change of sensitivity to proteolytic degradation. Antibodies to both homogeneous enzymes demonstrate a structural similarity for both types of subunit using the highly sensitive immunoblotting technique.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D002241:Carbohydrates / Q000032:analysis; D003593:Cytoplasm / Q000201:enzymology; D000939:Epitopes / Q000032:analysis; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000936:Antigen-Antibody Complex; D002241:Carbohydrates; D000939:Epitopes; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase