Mechanism of linear DNA circularization: formation of 'lasso'-like structures of pre-mRNA DNA copies.
An electron microscopic investigation of a DNA product synthesized on the pre-mRNA template using AMV reverse transcriptase in the absence of actinomycin D revealed linear single-stranded and double-stranded molecules, and, also, double-stranded branched as well as 'lasso'-like and circular structures. Models for the formation of such DNA structures are proposed.
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001853:Bone Marrow / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D008854:Microscopy, Electron; D008961:Models, Structural; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011817:Rabbits; D013698:Templates, Genetic
D004270:DNA, Circular; D012333:RNA, Messenger; D011061:Poly A; D012194:RNA-Directed DNA Polymerase