Monoamine metabolites in human cerebrospinal fluid. HPLC/ED method.
Catecholamines and indolealkylamines are of clinical interest in neurological and psychiatric disorders. We measured 3-methoxy-DOPA, 3-methoxy-4-hydroxyphenylglycol, dihydroxyphenylacetic acid, tryptophan, 5-hydroxyindoleacetic acid and homovanillic acid in human cerebrospinal fluid with a simple, sensitive , inexpensive, rapid and accurate procedure using high performance liquid chromatography coupled to an electrochemical detector. Patients with Parkinson's disease have a decrement in homovanillic acid that is reversed by treatment with L-3,4-dihydroxyphenylalanine. After this medication, 3-methoxy-DOPA is measurable in cerebrospinal fluid. Patients with depression show a decrease in serotonin turnover expressed by diminished 5-hydroxyindoleacetic acid content in cerebrospinal fluid. Depressed patients also show low levels of tryptophan. Monoamine metabolites are augmented in patients with subarachnoid hemorrhage.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid*; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003863:Depression / Q000134:cerebrospinal fluid; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid; D004563:Electrochemistry; D006018:Glycols / Q000134:cerebrospinal fluid*; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D008734:Methoxyhydroxyphenylglycol / Q000134:cerebrospinal fluid*; D010300:Parkinson Disease / Q000134:cerebrospinal fluid; D010648:Phenylacetates / Q000134:cerebrospinal fluid*; D013345:Subarachnoid Hemorrhage / Q000134:cerebrospinal fluid; D014364:Tryptophan / Q000134:cerebrospinal fluid*; D014443:Tyrosine / Q000031:analogs & derivatives*
D006018:Glycols; D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D014443:Tyrosine; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D004295:Dihydroxyphenylalanine; D014364:Tryptophan; C008404:3-methoxytyrosine; D006719:Homovanillic Acid