A rapid and sensitive assay for protein disulphide isomerase activity.
An assay procedure for the determination of protein disulphide isomerase activity is presented. The method is based on the reactivation of randomly cross-linked RNAase, the extent of RNAase reactivation being determined from the degradation of radioactively labelled RNA. The method is rapid and sensitive and allows one to test a large number of samples simultaneously.
D000818:Animals; D002642:Chick Embryo; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts; D007535:Isomerases / Q000032:analysis* / Q000378:metabolism; D008722:Methods; D019704:Protein Disulfide-Isomerases; D012313:RNA / Q000302:isolation & purification; D012260:Ribonucleases / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
D012313:RNA; D004492:Edetic Acid; D012260:Ribonucleases; D007535:Isomerases; D019704:Protein Disulfide-Isomerases