Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas.
Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red.
D000362:Agar; D002266:Carboxymethylcellulose Sodium; D002480:Cellulase / Q000032:analysis* / Q000302:isolation & purification; D003224:Congo Red; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D013194:Staining and Labeling
D003224:Congo Red; D000362:Agar; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium