An antiglobulin: IgG anti-IgE. Occurrence and specificity.
An IgG type of antibody directed against IgE has been studied in serum from healthy and allergic individuals. The technique used is based on a solid phase paper radioimmunoassay in which the discs were sensitized with purified IgE myeloma. After incubation with patient's serum, 125I anti-human IgG was added. The anti-IgE antibodies were partially blocked by endogenous IgE, indicating the presence of IgE-containing immune complexes in the serum. Heating of serum at 56 degrees C disrupted the immune complexes, thereby increasing the detectable levels of IgG anti-IgE. Significantly raised levels of anti-IgE antibodies were found in patients suffering from atopic disorders in comparison with the controls.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology; D000939:Epitopes; D006358:Hot Temperature; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007841:Latex Fixation Tests; D011863:Radioimmunoassay
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007074:Immunoglobulin G; D007073:Immunoglobulin E