Rapid separation and measurement of rat urinary kallikrein by high-performance liquid chromatography with a continuous flow enzyme detector.
Rat urinary kallikrein was separated by high-performance liquid chromatography (HPLC) using an ion-exchange or gel-permeation column. Kallikrein activity was monitored continuously with peptidase or esterase activity using a post-reactor system directly adapted to HPLC. A PTFE helically coiled tube served as the enzyme reactor vessel. Four and three peaks with peptidase and esterase activity, respectively, were detected on application of normal rat urine.
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001322:Autoanalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004950:Esterases / Q000652:urine; D007610:Kallikreins / Q000652:urine*; D008297:Male; D010447:Peptide Hydrolases / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D007611:Aprotinin; D004950:Esterases; D010447:Peptide Hydrolases; D007610:Kallikreins