Light form of Morris hepatoma gamma-glutamyltransferase.
Using solubilization with bromelain, the light form of gamma-glutamyltransferase was purified from Morris hepatoma 5123D. Some properties of this enzyme were compared to those of the light form rat kidney GGT. Anthglutin and its isomer inhibit competitively the former enzyme but non-competitively the latter. On zymograms of rat control sera, five GGT fractions were noted, but in sera of rats with hepatoma 5123D also the light form and the increase of GGT activity ain region of fraction II were observed. Only these two enzyme fractions react with antibody anti heavy form of Morris hepatoma GGT.
D000818:Animals; D000939:Epitopes; D005971:Glutamates / Q000494:pharmacology; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats; D011915:Rats, Inbred BUF; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000276:immunology / Q000378:metabolism*
D000939:Epitopes; D005971:Glutamates; D005723:gamma-Glutamyltransferase; C018830:anthglutin