[Study of the structural and functional properties of fibronectin--a high molecular weight plasma glycoproteins--by using monoclonal antibodies].
Functional and structural properties of fibronectin--high molecular weight glyco-protein from human plasma--were studied by monoclonal antibodies against fibronectin. It was shown that monoclonal antibodies against human plasma fibronectin exhibit a certain species specificity. Antigenic determinant for our monoclonal antibody is located in the central part of the protein polypeptide chain--in the structural domain. The monoclonal antibodies studied do not inhibit any tested functions of fibronectin. In contrast, polyclonal antibodies are not species specific and inhibit all fibronectin functions.
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000939:Epitopes / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis* / Q000097:blood / Q000276:immunology; D005780:Gelatin / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis* / Q000097:blood / Q000276:immunology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D006825:Hybridomas; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D011487:Protein Conformation; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005353:Fibronectins; D006023:Glycoproteins; D005780:Gelatin; D006493:Heparin
Izuchenie strukturno-funktsional'nykh svoĭstv fibronektina-- vysokomolekuliarnogo glikoproteida iz plazmy--pri pomoshchi monoklonal'nykh antitel.