Dynamics of keratin filaments and the intermediate filament distribution center during shape change in PtK1 cells.
Reorganization of intermediate filaments during cell spreading is examined by immunofluorescence, electron microscopy, and time-lapse video microscopy. A juxtanuclear cap, believed to correspond to the intermediate filament distribution center, was observed to be spatially related to the organization of the intermediate filament network as cells spread. A keratin cap was observed, which appeared spontaneously in motile PtK1 cells. Cap formation may be a consequence of retraction of intermediate filaments from the cytoplasm as cells move. The position of this juxtanuclear cap is related to the direction of movement, located on the side of the nucleus near the advancing edge of the cell. As the cell spreads, the cap disappears as the keratin filament network returns to the cytoplasm. Evidence presented here is consistent with the hypothesis that the distribution center mediates keratin filament organization during cell shape change.
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007633:Keratins / Q000276:immunology / Q000502:physiology*; D008854:Microscopy, Electron
D007633:Keratins