title: The human T cell antigen receptor is encoded by variable, diversity, and joining gene segments that rearrange to generate a complete V gene.
abstract: A cDNA clone YT35 , synthesized from poly(A)+ RNA of the human T cell tumor Molt 3, exhibits homology to the variable (V), joining (J), and constant (C) regions of immunoglobulin genes. We have isolated and sequenced the germ-line V and J gene segment counterparts to YT35 from a human cosmid library, and these failed to encode 14 nucleotides of the cDNA clone between the V and J regions. We postulate that these 14 nucleotides are encoded by a third gene segment analogous to the diversity (D) gene segments of immunoglobulin heavy chain genes. This T cell antigen receptor V gene appears to be assembled from three gene segments, V, D, and J, and accordingly most closely resembles immunoglobulin heavy chain V genes.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D007945:Leukemia, Lymphoid; D009154:Mutation; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D011110:Polymorphism, Genetic; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D011061:Poly A; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes