Anti-striated muscle antibody activity produced by Trypanosoma cruzi.
We have previously shown that Trypanosoma cruzi shares antigenic determinants with preparations of the calcium-sequestering adenosine triphosphatase of sarcoplasmic reticulum. The cross-reacting antigen (SRA) is also apparently present on the sarcolemma of cardiac myofibers. Using highly specific reference antisera to either the small membranes of T. cruzi or to a tryptic fragment of striated muscle SRA, it was shown that SRA is present in the striated muscle of animals representative of the evolutionary scale ranging from nonhuman primate to fish. The small membranes of nine different T. cruzi strains isolated from widely divergent areas of the American continents also reacted with the reference antisera. This indicates that SRA is present in these T. cruzi strains and may be prevalent among all T. cruzi strains. The shared T. cruzi-striated muscle antigen, SRA, may be a heteroantigen present in all T. cruzi strains and in the striated muscle of all classes of animals. Immunization of rabbits (three of five) or chickens (five pairs of five pairs) with striated muscle membrane preparations of different classes of animals, particularly those of nonhuman primate, chicken, and turtle, gave rise to IgG anti-allogeneic striated muscle antibody activity. Immunization of rabbits (four of nine) and chickens (five pairs of six pairs) with the small membranes of different T. cruzi strains also produced IgG anti-allogeneic striated muscle. These data indicate that T. cruzi shares cross-immunogenicity with striated muscle SRA. Since SRA is apparently present on the sarcolemma of cardiac myofibers, it may be implicated in the immunopathogenesis of Chagas' disease.
D000818:Animals; D005075:Biological Evolution; D002645:Chickens / Q000276:immunology; D002522:Chlorocebus aethiops / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006054:Goldfish / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008861:Microsomes / Q000276:immunology; D009132:Muscles / Q000276:immunology*; D011817:Rabbits; D011898:Ranidae / Q000276:immunology; D012519:Sarcoplasmic Reticulum / Q000276:immunology; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000276:immunology*; D014426:Turtles / Q000276:immunology
D000939:Epitopes; D007074:Immunoglobulin G