Enzyme immunoassay of benzo[a]pyrene conjugated to DNA, RNA and microsomal proteins using a monoclonal antibody.
An enzyme immunoassay for the detection of benzo[a]pyrene covalently conjugated to macromolecules has been developed. The monoclonal antibody, raised through in vitro immunization reacted with benzo[a]pyrene metabolites bound to DNA, RNA and proteins. The lower detection limit for the assay was 1 pmol for benzo[a]pyrene bound to DNA or RNA, and 5 pmol when bound to protein.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000032:analysis* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats
D000911:Antibodies, Monoclonal; D001580:Benzopyrenes; D011506:Proteins; D001564:Benzo(a)pyrene; D012313:RNA; D004247:DNA