title: Non-protein-bound iron within bacterial cells and the action of bleomycin.
abstract: Damage to DNA by bleomycin in vitro is entirely dependent on the presence of an iron salt and molecular oxygen. This same reaction has been used to measure non-protein-bound iron in different bacterial species. Although several of the species examined were insensitive to the toxic effects of bleomycin, they all contained a concentration of non-protein-bound iron which should have been sufficient to allow iron-dependent damage to DNA. Other factors such as permeability or inactivation of the drug must explain their apparent insensitivity.
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D001761:Bleomycin / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D013291:Streptococcus / Q000187:drug effects / Q000378:metabolism*; D013298:Streptococcus sanguis / Q000378:metabolism
chemical_list: D001761:Bleomycin; D007501:Iron