Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates.
Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.
D000818:Animals; D001370:Axonal Transport; D001692:Biological Transport; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D006735:Horseradish Peroxidase; D037102:Lectins; D009132:Muscles / Q000294:innervation*; D009445:Neuroanatomy / Q000379:methods*; D009475:Neurons, Afferent; D010544:Peroxidases; D051381:Rats; D012867:Skin / Q000294:innervation*; D013116:Spinal Cord / Q000033:anatomy & histology*; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase; D010544:Peroxidases