Pyrimidine metabolism in Tritrichomonas foetus.
The pyrimidine metabolism of Tritrichomonas foetus (KV 1) was studied using whole cells and cell homogenates. Pyrimidines and pyrimidine nucleosides were readily incorporated into nucleic acids. Orotate and aspartate were not incorporated into pyrimidine bases. Enzymes of the pyrimidine salvage pathway (i.e., thymidine and uridine phosphorylases and uridine kinase) were detected in trophozoite homogenates, but the activities of de novo pyrimidine synthesis enzymes (i.e., carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase and dihydroorotate dehydrogenase) were below the level of detection in these same homogenates. The evidence presented supports the proposal that T. foetus is incapable of synthesizing pyrimidines de novo but is capable of salvaging preformed pyrimidines and pyrimidine nucleosides from the growth medium and that enzymes of this parasite's pyrimidine salvage pathway are not organelle-associated.
D000818:Animals; D003562:Cytidine / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D013936:Thymidine / Q000378:metabolism; D013939:Thymidine Phosphorylase / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014318:Tritrichomonas / Q000201:enzymology / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014541:Uridine Kinase / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000378:metabolism
D011506:Proteins; D011741:Pyrimidine Nucleosides; D011743:Pyrimidines; D014498:Uracil; D003562:Cytidine; D012313:RNA; D004247:DNA; D014543:Uridine Phosphorylase; D013939:Thymidine Phosphorylase; D014541:Uridine Kinase; D013941:Thymine; D013936:Thymidine; D014529:Uridine