title: Idiotypes and allotypes on Ia-binding alloactivated Lyt-1+,2-,3- T cells are coded for by genes linked to the igh-1 allotype locus.
abstract: Xenogeneic antisera, designated 5936 and 6036, have been used in this laboratory to define 5936-idiotypes (Id) and 6036-T cell receptor (Tcr) allotype determinants, respectively, on populations of B6 anti-CBA MLC T blasts. The present experiments were carried out to determine a) whether T lymphocytes that bear 5936-Id also express antiserum 6036-defined Tcr allotypes; b) if so, what is the Lyt phenotype and c) specificity of this T lymphocyte population, and d) whether the genes coding for 5936-Id and 6036-allotypes are linked to immunoglobulin (Ig) allotype genes. The results showed that 5936-Id-bearing T lymphocytes were included in a subset of cells that expressed antiserum 6036-defined Tcr allotypic determinants and the Lyt-1+,2-,3- phenotype. By genetic segregation analysis it was shown that both 5936-Id and antiserum 6036-defined allotypic determinants on T cells were linked to Igh-1b allotypes. 5936-Id determinants were expressed on T lymphocytes of Igh-1b allotype strains, specific for allogeneic I-Ak but not on those specific for I-Ak/Ek molecules. In contrast, 6036-Tcr allotype determinants were present on T lymphocytes from Igh-1b allotype strains, specific for either I-Ak or I-Ak/Ek molecules. The results support the hypothesis that 5936-Id determinants are present only on a subset of anti-I-Ak T cell receptors, whereas 6036-Tcr allotype determinants are shared among T cell receptors of several unrelated specificities from Igh-1b allotype-bearing strains.
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics*; D003433:Crosses, Genetic; D000939:Epitopes / Q000235:genetics; D005260:Female; D005802:Genes, MHC Class II; D005815:Genetic Code; D008040:Genetic Linkage; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology
chemical_list: D000950:Antigens, Ly; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes