Evidence for the coupling of T7 DNA injection with its transcription during infection.
The kinetics of T7 phage DNA transfer from the virion into E. coli during infection in the presence of chloramphenicol was compared under the same conditions with the kinetics of its in vivo transcription. The results obtained allow us to conclude that these two processes are tightly coupled.
D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000096:biosynthesis; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases