Differences between normal antithrombin III and antithrombin III Padua as determined by laser nephelometry.
Laser nephelometry was used to characterize an abnormal antithrombin III (AT III Padua) in comparison with normal antithrombin. Heparinized (0.2 IU/ml) or non-heparinized AT III Padua plasma reacts with Laser nephelometry antithrombin III antiserum in a different way compared with pooled normal plasma. There is in fact a slower antigen-antibody reaction during the first 40-45 min both in AT III Padua heparinized and non-heparinized plasmas, compared with pooled normal plasma; then the kinetics overlap. On the contrary the concentrations of antithrombin III Padua in percent correlate well with those obtained by Mancini's and Laurell's methods. These data indicate that Laser nephelometry is suitable for AT III antigen determinations and may also supply useful information for the characterization of abnormal clotting factors.
D000990:Antithrombin III / Q000032:analysis*; D006801:Humans; D007834:Lasers; D009391:Nephelometry and Turbidimetry / Q000379:methods
C037622:antithrombin III Padua; D000990:Antithrombin III