Effects of human gamma interferon on cell growth, replication of virus and induction of 2'-5'oligoadenylate synthetase in three human lymphoblastoid cell lines and K562 cells.
Human alpha interferon (IFN-alpha) and beta interferon (IFN-beta) showed antiviral and anticellular effects on human lymphoblastoid Daudi and P3HR-1 cells, but up to 1,000 units/ ml of gamma interferon (IFN-gamma) showed no such effect. Though a fairly high level of dsRNA-dependent 2'-5'-oligoadenylate synthetase (2-5A synthetase) was found in Daudi cells, treatment of these cells with IFN-alpha and beta enhanced the enzyme level in cells at least four-fold, but IFN-gamma did not show any such effect. Lymphoblastoid Raji cells were insensitive to the antiviral and anticellular activities of IFN-alpha, beta and gamma, but 2-5A synthetase was induced in cells by the treatment with IFN-alpha and beta, though the enzyme level was lower than that found in interferon-treated Daudi cells. Human leukemic K562 cells were completely insensitive to IFN-alpha, beta and at the same time to IFN-gamma with regard to the antiviral, anticellular activities and to the induction of 2-5A synthetase.
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D002460:Cell Line; D004790:Enzyme Induction; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D014779:Virus Replication / Q000187:drug effects*
D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase