Effect of cerulenin on macromolecule synthesis in chemoheterotrophically and photoheterotrophically grown Rhodopseudomonas sphaeroides.
The antibiotic cerulenin causes the immediate cessation of phospholipid biosynthesis in both chemoheterotrophic and photoheterotrophic cultures of Rhodopseudomonas sphaeroides. Macromolecule biosynthesis in photoheterotrophic cells was unaffected by cerulenin for the first 2 h after antibiotic addition and then continued at a reduced rate for an additional 8 h. In contrast, macromolecule biosynthesis in chemoheterotrophic cells was severely affected by cerulenin within the first 2 h of treatment. Pulse-labeling of protein after cerulenin addition revealed that all subcellular fractions were equally affected by the action of cerulenin with chemoheterotrophic cell fractions more profoundly affected than those derived from photoheterotrophic cells. Protein insertion into the intracytoplasmic membrane of photoheterotrophic cells continued for up to 6 h after the onset of cerulenin treatment. Residual macromolecule synthesis was correlated with the presence of the photosynthetic membrane system under all conditions of growth.
D000935:Antifungal Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002569:Cerulenin / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D007700:Kinetics; D008027:Light; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012242:Rhodobacter sphaeroides / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D000935:Antifungal Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D002569:Cerulenin