Fractionation of membrane proteins on phenylboronic acid-agarose.
Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.
D001897:Boronic Acids; D002847:Chromatography, Agarose / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D003840:Deoxycholic Acid / Q000494:pharmacology; D006680:HLA Antigens / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood; D008214:Lymphocytes / Q000032:analysis; D008565:Membrane Proteins / Q000097:blood* / Q000276:immunology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives; D000509:alpha-Fetoproteins / Q000302:isolation & purification
D001897:Boronic Acids; D006680:HLA Antigens; D008565:Membrane Proteins; D011947:Receptors, Antigen, B-Cell; D000509:alpha-Fetoproteins; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; C035597:phenylboronic acid-sepharose; D017830:Octoxynol; D012685:Sepharose; C010615:Nonidet P-40; C010686:benzeneboronic acid