Stimulus specificity of the generation of leukotrienes by dog mastocytoma cells.
Isolated dog mastocytoma cells sensitized with dog anti-ragweed IgE and challenged with ragweed antigen or incubated with ionophore A23187 or the carboxy-terminal dodecapeptide of platelet factor 4, PF4(59-70), release histamine and concurrently generate leukotrienes B4, C4, and D4. In contrast, the exposure of mastocytoma cells to 0.1-3 micrograms/ml of 15-hydroxyeicosatetraenoic acid (15-HETE) stimulates selectively the generation of leukotrienes, in the absence of histamine release, while 0.1-1 micrograms/ml of compound 48/80 releases histamine without enhancing the generation of leukotrienes. That natural stimuli are capable of selectively activating one synthetic or secretory compartment of mast cells suggests that separate subsets of receptors as well as different biochemical events may serve to mobilize each class of mediators.
D000485:Allergens / Q000276:immunology; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D004285:Dogs; D006636:Histamine Release; D006893:Hydroxyeicosatetraenoic Acids; D007700:Kinetics; D007975:Leukotriene B4 / Q000096:biosynthesis; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D010978:Platelet Factor 4 / Q000276:immunology; D013189:SRS-A / Q000096:biosynthesis*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000485:Allergens; D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D013189:SRS-A; D007975:Leukotriene B4; D010978:Platelet Factor 4; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid