Localization of murine Igh-1a allotypic determinants by using a panel of mouse myeloma variant immunoglobulins.
A number of monoclonal antibodies are available that are reactive with distinct mouse immunoglobulin allotypic determinants. By determining which ones are present on a panel of hybrid IgG2b-IgG2a immunoglobulins, we have localized some of the allotypic determinants present on the IgG2a heavy chain of the "a" allotype (Igh-1a proteins). In particular, one group of determinants--Ig(1a)9.8 (20.6B8), 17.2 (20.19.2), and 14.4 (21.74.4)--has been placed in the CH2 domain. A second group--Ig(1a)8.3 (20.8.3), 21.2 (20.11.2), and 15.3 (21.66.3)--is located in a segment spanning the C terminal 8 residues of the CH2 domain and the complete CH3 domain.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000235:genetics*; D014644:Genetic Variation; D007126:Immunoglobulin Allotypes / Q000235:genetics* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000235:genetics* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D009194:Myeloma Proteins; C017551:myeloma immunoglobulins