Plasmodium chabaudi antigens synthesized in an mRNA-dependent cell-free translation system.
Intact RNAs were isolated from Plasmodium chabaudi growing synchronously in mice at different stages of the erythrocytic cycle. Translation of the mRNAs using rabbit reticulocyte lysate showed stage-specific patterns comparable to those observed in vivo. Many antigens of P. chabaudi are produced by translation in the rabbit reticulocyte lysate. Some other antigens are predominantly synthesized at a particular stage, indicating that the mRNA populations differ from one stage to another.
D000818:Animals; D000941:Antigens / Q000096:biosynthesis*; D002474:Cell-Free System; D005260:Female; D005815:Genetic Code; D051379:Mice; D010961:Plasmodium / Q000276:immunology*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics*; D012335:RNA, Ribosomal / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis
D000941:Antigens; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012313:RNA