Aberrant migration of lipopolysaccharide in sodium dodecyl sulfate/polyacrylamide gel electrophoresis.
Purified lipopolysaccharides of salmonellae strains were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Pre-electrophoresis of polyacrylamide gels had no apparent effect on one-dimensional silver-stained lipopolysaccharide profiles. However, without pre-electrophoresis, two-dimensional and three-dimensional patterns contained numerous bands with varied migration patterns compared to those in the one-dimension gels. The lipopolysaccharide was altered within the polyacrylamide gel during electrophoresis. Pre-electrophoresis of gels eliminated aberrant migration patterns.
D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D012475:Salmonella / Q000032:analysis; D012486:Salmonella typhimurium / Q000032:analysis; D012834:Silver; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D008070:Lipopolysaccharides; D012967:Sodium Dodecyl Sulfate; D012834:Silver