title: An improved method for increasing the resolution and sensitivity of silver staining of nucleic acid bands in polyacrylamide gels.
abstract: Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D012313:RNA / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D012834:Silver; D013194:Staining and Labeling
chemical_list: D012329:RNA, Bacterial; D012834:Silver; D012313:RNA; D012343:RNA, Transfer