In vitro synthesis of subunits of bacterial luciferase in an Escherichia coli system.
RNA extracted from the luminescent bacterium Vibrio harveyi was translated in an Escherichia coli system. RNA from highly luminescent cells produced both alpha and beta subunits of luciferase in vitro, as confirmed by immunoprecipitation and partial proteolysis. RNA from cells before induction of luminescence did not direct luciferase synthesis.
D004926:Escherichia coli / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis*; D008163:Luminescent Measurements; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology* / Q000502:physiology
D012329:RNA, Bacterial; D012333:RNA, Messenger; D008156:Luciferases