A granulocyte reactive monoclonal antibody, 1G10, identifies the Gal beta 1-4 (Fuc alpha 1-3)GlcNAc (X determinant) expressed in HL-60 cells on both glycolipid and glycoprotein molecules.
1G10, a monoclonal IgM antibody that identifies a differentiation antigen on human granulocytes and a subpopulation of monocytes, was found to react specifically with glycosphingolipids bearing the Gal beta 1-4(Fuc alpha 1-3)GlcNAc hapten (X determinant). This carbohydrate determinant was found on both glycolipid and glycoprotein molecules isolated from HL-60 cells (a promyelocytic leukemia cell line). Thus, this highly conserved carbohydrate-defined determinant previously described on mouse embryonic and mouse and human carcinoma cells is also expressed as a tissue-specific differentiation antigen on normal human granulocytes.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002855:Chromatography, Thin Layer; D000939:Epitopes; D006017:Glycolipids / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; D006023:Glycoproteins; D006241:Haptens