Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.
Study of the differentiation of immunoglobulin-producing B lymphocytes has been hampered by the inability to maintain homogeneous populations of precursor cells in vitro. We describe here that interleukin-3 supports the growth of freshly isolated fetal liver pre-B cells and the long-term culture of interleukin-3 dependent pre-B-cell clones that can be induced to mature into antibody secreting cells in vitro.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D002999:Clone Cells; D004261:DNA Replication / Q000187:drug effects; D000939:Epitopes / Q000032:analysis; D005796:Genes; D007075:Immunoglobulin M / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D007377:Interleukin-3; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype
D000954:Antigens, Surface; D000939:Epitopes; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D007377:Interleukin-3; D008222:Lymphokines