Analysis of structural properties and cellular distribution of avian Ia antigen by using monoclonal antibody to monomorphic determinants.
In this report, we describe the analysis of Ia-like antigens in the chicken by using a monoclonal antibody (CIa-1) reactive with monomorphic determinants of the Ia-like (B-L) antigens. This antibody reacts with determinants on B cells in all avian species tested, but does not detect antigens on lymphocytes of representative mammals, reptiles, and amphibians. In addition to B cells, this antibody defines a subpopulation of the monocyte-macrophage series and reacts with mitogen-activated T cells. Immunochemical analysis indicates that the CIa-1 reactive antigen is a 65,000-dalton glycoprotein consisting of an alpha-chain of 32,000 daltons noncovalently bound to a beta-chain of 27,000 daltons. Under nonreducing conditions, the beta-chain migrates with slightly faster mobility. Two-dimensional gel analysis indicates that the beta-chain is the more heterogeneous of the two chains. Thus, the antigen detected by CIa-1 antibody is similar in cell distribution and structure to the murine Ia antigens and human DR antigens. During in ovo development, Ia+Ig- cells were not found in the yolk sac but were detected in the spleen, mesonephros, and bursa of 9-day embryos. Two populations of Ia+Ig- cells were identified in the bursa: 40 to 60% of the bursacytes, mostly larger cells, exhibited brighter immunofluorescence reactivity than the smaller bursacytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000502:physiology; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002060:Bursa of Fabricius / Q000166:cytology / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D002642:Chick Embryo; D002645:Chickens; D010856:Columbidae; D004372:Ducks; D000939:Epitopes / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D005399:Fishes; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008650:Mesonephros / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011784:Quail; D011894:Rana pipiens; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014426:Turtles
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; C041136:Ia-like antigens, avian