Evaluation of 3H-histamine incorporation into human peripheral blood leukocytes.
We have examined the use of 3H-histamine for evaluating in vitro basophil responses. Incubation of 3H-histamine for 4 h with basophil-enriched mononuclear fractions containing autologous plasma, resulted in the best incorporation of the label. Even so, less than 3% of the added label was actually recovered and other leukocytes also bound or incorporated 3H-histamine. Labeled mononuclear preparations failed to release 3H-histamine when challenged with zymosan-activated serum or anti-IgE, but endogenous histamine released under the same experimental conditions could be determined fluorometrically. The calcium ionophore, A23187, however, did cause a dose-dependent release of 3H-histamine. We conclude that 3H-histamine is not preferentially incorporated into basophil granules, and that this technique cannot be used for assessing allergen and/or IgE-mediated basophil responses.
D001491:Basophils / Q000378:metabolism; D002469:Cell Separation; D006098:Granulocytes / Q000378:metabolism; D006632:Histamine; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D007158:Immunologic Techniques; D007700:Kinetics; D007962:Leukocytes / Q000145:classification / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism
D006632:Histamine