Differential staining of the X-chromosome during meiosis of orthoptera by a silver impregnation procedure.
Differential staining of the X chromosome of Orthoptera, which normally cannot be distinguished from the autosomes during the more condensed stages of meiosis, has been achieved by a silver impregnation procedure involving a pretreatment with 2 x SSC at 60 degrees C. The results suggest that the saline citrate solution preferentially extracts proteins from the X chromosome. This method may be useful for distinguishing chromosome regions composed of facultative heterochromatin in Orthoptera.
D000818:Animals; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure; D008297:Male; D008540:Meiosis; D009987:Orthoptera / Q000648:ultrastructure*; D012730:Sex Chromosomes / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods*; D014960:X Chromosome / Q000648:ultrastructure*
D006570:Heterochromatin