title: A simplified method for coliphage detection in natural waters.
abstract: The ARCAT (A Rapid Coliphage Analysis Technique) method for detecting coliphages in water has been modified. Modifications to the original method include media optimization, the use of frozen host cultures, the use of a single agar coliphage assay and optimized plaque resolution with 2, 3, 4-triphenyltetrazolium chloride. Detection of 5 coliphages per 100 ml of water sample is accomplished in 6 hours for rapid estimation of water quality.
mesh_terms: D003090:Coliphages / Q000302:isolation & purification*; D003470:Culture Media; D004926:Escherichia coli / Q000502:physiology; D005615:Freezing; D008828:Microbiological Techniques; D010948:Viral Plaque Assay; D014871:Water Microbiology
chemical_list: D003470:Culture Media