[The effect of the antibiotic edeine on tRNA interaction with A, P and E sites of Escherichia coli 70S ribosomes].
Edeine inhibits poly(U)-dependent binding of tRNAPhe to the P and A sites simultaneously, both on 30S subunits and 70S ribosomes. Hence, edeine cannot be considered as antibiotic, "complementary" to tetracycline for selective adsorption of tRNA only to the P or to the A site. Further, edeine decreases the affinity constant of tRNAPhe for the P-site by more than two orders of magnitude, no matter poly(U) is present or not. Neither edeine nor tetracycline affect interaction of deacylated tRNAPhe with the E-site of E. coli 70S ribosomes.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D004486:Edeine / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D004486:Edeine; D012343:RNA, Transfer
Osobennosti vliianiia antibiotika édeina na vzaimodeĭstvie tRNK s uchastkami A, P i E 70S ribosom Escherichia coli.