Hypoxia stimulates myocardial RNA synthesis and histone acetylation.
Fifteen minutes of hypoxic perfusion (95%N2:5%CO2) produced a 75%-80% decrease in contraction rate with a slight increase in coronary flow. During aerobic recovery (95%O2:5%CO2), hemodynamic function returned to control values within 3-5 min of perfusion. Uridine incorporation into total tissue RNA was stimulated 78% after 60 min of recovery perfusion. The acetylation of total nucleohistones was significantly (P .05) increased after 40 and 60 min of aerobic perfusion. These data suggest that 1) a brief period of hypoxia was sufficient to stimulate RNA synthesis and 2) enhanced histone acetylation was associated with increased myocardial RNA synthesis.
D000107:Acetylation; D000818:Animals; D001834:Body Water / Q000378:metabolism; D003326:Coronary Circulation; D003327:Coronary Disease / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D006657:Histones; D012313:RNA; D014529:Uridine