SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity.
We have developed a monoclonal antibody with HLA-DR5 serologic specificity. The antibody, SFR3-DR5, binds specifically to DR5-positive lymphoblastoid cell lines, and immunoprecipitates alpha- and beta-chains characteristic of DR antigens from them. Cytotoxic activity of the antibody segregates with the DR5-bearing haplotype in a family. The antibody reacted with the cells of 16 of 17 DR5 individuals and was negative on all DR5-negative cells tested. SFR3-DR5 reacted weakly with PWM-activated cells of the single DR5 individual whose B lymphocytes were unreactive with the monoclonal antibody by cytotoxicity. Possible interpretations of these results are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001666:Binding Sites, Antibody; D002460:Cell Line; D011232:Chemical Precipitation; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D015805:HLA-DR5 Antigen; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015805:HLA-DR5 Antigen; D000949:Histocompatibility Antigens Class II