Effect of ions on alpha-amylase activity of human granulocytes.
The effect of the salts NaCl, KCl, CsCl, NH4Cl, NaBr, CH3COONa, NaNO3 and Na2CO3 on the alpha-amylase activity of dialysed and nondialysed human granulocyte extracts was measured. The optimum activity was found at the 0.02 M salt concentrations. The highest enzyme activity was found with NaCl, CsCl, NH4Cl and KCl. The apoamylase was successfully reactivated with the cations Ca2+, Ba2+, Mn2+, while reactivations with Co2+, Ni2+, Mg2+ are below the limits of significance.
D000681:Amylases / Q000097:blood*; D002118:Calcium / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007477:Ions; D012492:Salts / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D000516:alpha-Amylases / Q000097:blood*
D007477:Ions; D012492:Salts; D012965:Sodium Chloride; D000681:Amylases; D000516:alpha-Amylases; D002118:Calcium