[Use of DEAE-Sephadex A-50 microcarriers for reproduction of the mumps virus].
Reproduction of the vaccine L-3 strains of mumps virus was studied in cultures of continuous Vero cells and in primary cultures of Japanese quail embryos (JQE) growing on DEAE-Sephadex A-50 microcarriers. The Vero cell culture multiplies actively on the microcarrier surface giving more than a 20-fold increase in 8 days. Mumps virus showed a high reproductive capacity in Vero cell culture and in primary JQE cells. Mumps virus-infected Vero cells produce 5-6 pools of virus-containing material with a mean infectious titre 8.2-8.3 lg HAE50/ml. The primary JQE culture infected with mumps virus can yield 2-3 pools of virus-containing material. The intensity of mumps virus replication in the latter directly depends on the multiplicity of infection. Hemadsorption test could be performed in mumps virus-infected cell cultures on microcarriers.
D000818:Animals; D002478:Cells, Cultured; D003370:Coturnix; D003637:DEAE-Dextran / Q000031:analogs & derivatives*; D003911:Dextrans / Q000494:pharmacology*; D006382:Hemadsorption; D009109:Mumps virus / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D003911:Dextrans; C027184:DEAE-Sephadex A-50; D003637:DEAE-Dextran
Ispol'zovanie mikronositeleĕ DEAE-Sefadeks A-50 dlia reproduktsii virusa parotita.