[Densitometric determination of nucleic acids in gels after electrophoresis].
A technique for the quantitative determining of nucleic acids after electrophoresis by double-wave densitometry in UV spectrum by scanning agar gel along both axes was developed. Optical characteristics of agar and acrylamide-agar gels are given. The range of quantities measured is 6 divided by 30 micrograms of DNA per gel; the standard deviation adjusted to 1 microgram is less than +/- 3%.
D000818:Animals; D004247:DNA / Q000032:analysis*; D003720:Densitometry / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005782:Gels; D012313:RNA / Q000032:analysis*; D051381:Rats; D013950:Thymus Gland / Q000032:analysis; D014466:Ultraviolet Rays
D005782:Gels; D012313:RNA; D004247:DNA
Densitometricheskoe opredelenie nukleinovykh kislot v geliakh posle élektroforeza.