The action of anticlastogens in human lymphocyte cultures and its modification by rat liver S9 mix. I. Studies with AET and sodium fluoride.
The action of beta-aminoethylisothiouronium bromide hydrobromide (AET) and sodium fluoride (NaF) on the clastogenic activity of Trenimon, cyclophosphamide, and bleomycin was tested on cultures of human peripheral lymphocytes with and without the addition of rat liver S9 mix. In addition, the influence of both anticlastogens on the SCE-inducing activity of Trenimon and cyclophosphamide was examined under the same conditions. In the absence of S9 mix both substances displayed the known anticlastogenic action when TR was the standard clastogen but acted coclastogenically in the experiments with BM. Under the influence of rat-liver S9 mix this action on TR-induced chromosome damage was decreased and only a slight anticlastogenic effect was observed in the experiments with activated cyclophosphamide. S9-activated BM lost some of its strong chromosome-damaging effect and AET proved clearly anticlastogenic under these test conditions. AET displayed a slight decreasing effect on SCE induced by TR, but had no effect on CP-induced SCE. No anti-SCE effect at all was found in the experiments with NaF. Detailed analyses revealed different actions of both anticlastogens on the different types of structural chromosome damage.
D000818:Animals; D001711:Biotransformation; D001761:Bleomycin / Q000494:pharmacology; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D006801:Humans; D008677:Metaphase / Q000187:drug effects; D008862:Microsomes, Liver / Q000378:metabolism*; D009154:Mutation / Q000187:drug effects*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology*; D014228:Triaziquone / Q000494:pharmacology; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D001761:Bleomycin; D000338:beta-Aminoethyl Isothiourea; D003520:Cyclophosphamide; D012969:Sodium Fluoride; D014228:Triaziquone