Activity stain for pyruvate decarboxylase in polyacrylamide gels.
A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates within a few minutes. The more or less opaque bands can be viewed and photographed against a dark background and scanned in a densitometer. The detection limit is at about 10 mU pyruvate decarboxylase when the gels are bathed in the staining solution for 20 min. Several other methods were tested and failed to produce satisfactory results.
D002262:Carboxy-Lyases / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D011767:Pyruvate Decarboxylase / Q000302:isolation & purification*; D012440:Saccharomyces / Q000201:enzymology; D013194:Staining and Labeling; D014908:Triticum / Q000201:enzymology
D002262:Carboxy-Lyases; D011767:Pyruvate Decarboxylase