Avian myeloblastosis reverse transcriptase deacylates tryptophanyl-tRNA.
Tryptophanyl-tRNA can be used as a primer for RNA-dependent DNA synthesis by avian reverse transcriptase. We have determined that whereas the retroviral polymerase is not by itself capable of deacylating Trp-tRNATrp, the ester linkage between the 3' OH of the ribose moiety and the aminoacid can be very efficiently hydrolyzed when both the polymerase and the viral 35 S RNA are present.
D001354:Avian Leukosis Virus / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000378:metabolism*; D007700:Kinetics; D012343:RNA, Transfer / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D012343:RNA, Transfer; D012194:RNA-Directed DNA Polymerase