An antigen-specific signal is required for the activation of second-order suppressor T cells in the regulation of delayed-type hypersensitivity to 2,4,6-trinitrobenzene sulfonic acid.
Suppressor T cells (Ts-1) induced with trinitrophenyl (TNP)-conjugated syngeneic spleen cells (TNP-SC) can be enriched on antigen-coated plates and are afferent suppressors. In addition, these suppressor cells produced soluble suppressor factors (TsF) that were active in vivo. Therefore, the Ts-1 cells in the TNP system are very similar to the Ts-1 cells in other systems we have studied earlier. Further characterization of these TsF-1 revealed that TsF-1 obtained from TNP-SC-induced Ts-1 is major histocompatibility complex restricted in its activity. Injection of TNP-specific TsF-1 into naive mice did not induce Ts-2 unless additional corresponding antigen was provided. Moreover, the Ts-2 cells induced by administration of both TsF-1 and trinitrobenzene sulfonic acid were antigen specific rather than antiidiotypic.
D000818:Animals; D001665:Binding Sites; D000939:Epitopes; D006968:Hypersensitivity, Delayed / Q000175:diagnosis / Q000276:immunology*; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000235:genetics / Q000502:physiology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009578:Nitrobenzenes / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013154:Spleen / Q000166:cytology; D013491:Suppressor Factors, Immunologic; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000276:immunology*
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines; D009578:Nitrobenzenes; D011948:Receptors, Antigen, T-Cell; D013491:Suppressor Factors, Immunologic; D014302:Trinitrobenzenesulfonic Acid