Studies on pathogenicity and immunogenicity of cold adapted variants of influenza virus.
Cold adapted variants of influenza virus, replicated at 37, 35, 33 and 30 degrees C, were tested for capability to multiply in ferrets and to induce H and N antibodies. Virus grown at 30 degrees C differed from the other in electrophoretic mobility of its polypeptides in PAGE, in inducing higher level of N antibodies, and in higher intensity of immunofluorescence reaction in the respiratory tract.
D000222:Adaptation, Physiological; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D002642:Chick Embryo; D003080:Cold Temperature; D005289:Ferrets; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D008168:Lung / Q000382:microbiology; D009975:Orthomyxoviridae / Q000276:immunology / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000382:microbiology*; D014779:Virus Replication
D000914:Antibodies, Viral