Enhancement of viral RNA-induced interferon production of L cells treated with insulin and amphotericin B methyl ester.
A study was initiated to develop a more efficient method of producing high-titered L cell interferon. The induction of interferon by viral infectious RNA could be augmented by pretreating the cell cultures with insulin and infecting in the presence of Amphotericin B methyl ester (AmBME). L cells were treated for 24 hr with different concentrations of insulin and then challenged with MM virus RNA suspended in a balanced salt solution with and without AmBME. The fluids were collected 48 hr after infection and assayed for interferon activity. Interferon production was markedly increased in insulin-treated cultures and that the increase was dose-dependent.
D000666:Amphotericin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004686:Encephalomyocarditis virus; D007328:Insulin / Q000494:pharmacology*; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D007739:L Cells; D012367:RNA, Viral / Q000494:pharmacology
D007328:Insulin; D007369:Interferon Inducers; D012367:RNA, Viral; C017501:methylamphotericin B; D000666:Amphotericin B; D007372:Interferons