Idiotypic heterogeneity of the cross-reactive idiotype associated with the anti-p-azophenylarsonate antibodies of BALB/c mice.
The cross-reactive idiotype (CRI) associated with the BALB/c antibody response against the p-azophenylarsonate (Ar) hapten was studied by analyzing hybridoma anti-Ar derived from BALB/c mice. The BALB/c CRI (CRIc) was previously thought to be a single idiotype as defined by rabbit anti-idiotype. CRIc has been resolved into at least two separate families of anti-Ar antibodies that are unrelated idiotypically to each other. Analysis of the 5AF6 family revealed considerable idiotypic heterogeneity, including a number of public idiotopes that appeared to be primarily associated with combinatorial idiotopes (requiring H + L chains) or L chain-specific idiotopes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015478:Antigens, Heterophile / Q000276:immunology*; D001391:Azo Compounds / Q000276:immunology*; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006241:Haptens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007106:Immune Sera / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D001391:Azo Compounds; D000939:Epitopes; D006241:Haptens; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D010132:p-Azobenzenearsonate