title: Modulation of Schistosoma mansoni egg-induced granuloma formation. III. Evidence for an anti-idiotypic, I-J-positive, I-J-restricted, soluble T suppressor factor.
abstract: Modulation of pathogenic egg-induced hepatic granuloma formation in chronically Schistosoma mansoni-infected mice is an immunoregulatory process. Adoptive transfer and in vitro studies have demonstrated that this suppression involves various T lymphocyte circuitries, and the participation of soluble suppressor factors has recently been noted in these systems. The present study has partially characterized a soluble suppressive activity extracted from the thymus glands of chronically infected mice (SmTsF) that modulates granuloma formation in acutely infected mice. The suppressive effect of SmTsF could be administered by multiple i.v. injections or by slow release from osmotic minipumps implanted i.p. Homologous and reciprocal transfers of SmTsF prepared from B10.A(3R) and B10.A(5R) donors indicated that SmTsF-induced suppression required homology between the donor and recipient at the I-J subregion of the major histocompatibility complex. Furthermore, the use of immunoabsorbents prepared with anti-I-Jk and anti-I-Jb sera demonstrated that CBA/J (H-2k) SmTsF was retained by, and could be recovered from, anti-I-Jk insoluble columns, but was unaffected by parallel treatment with anti-I-Jb sera. Subsequent immunoabsorbent studies showed that SmTsF did not bind to soluble egg antigenic (SEA) columns, and thus demonstrated a lack of idiotype, anti-antigen activity. However, columns prepared by using anti-SEA IgG from chronically infected syngeneic mice retained SmTsF suppressive activity, and it could be recovered by alkaline elution. These data are compatible with an interpretation that the suppressive activity expressed anti-idiotypic reactivity. Thus a thymus extract obtained from chronic, modulated, S. mansoni-infected mice can induce granuloma suppression in acutely infected mice. This activity is associated with an I-J determinant-bearing, possibly anti-idiotypic moiety or moieties. These observations further implicate some of the Ts cascades reported in other systems in the regulation of cell-mediated pathogenesis in chronic experimental schistosomiasis.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage / Q000235:genetics / Q000502:physiology; D000939:Epitopes / Q000276:immunology; D005260:Female; D005802:Genes, MHC Class II; D006099:Granuloma / Q000209:etiology* / Q000276:immunology / Q000628:therapy; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008109:Liver Diseases, Parasitic / Q000209:etiology / Q000235:genetics / Q000276:immunology*; D008222:Lymphokines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010063:Ovum / Q000276:immunology; D012550:Schistosoma mansoni / Q000254:growth & development; D012552:Schistosomiasis / Q000150:complications / Q000235:genetics / Q000276:immunology*; D013491:Suppressor Factors, Immunologic
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic