Interferon production associated with the ageing of primary chick embryo cells is not caused by priming.
The presence of a low level of interferon (IFN) activity was demonstrated in aged culture medium of primary chick embryo (CE) cells. The endogenous cytoplasmic level of 2',5'-oligoadenylate (2',5'-A) synthetase activity also increased during cell ageing. This increase was suppressed by addition of antiserum against chick IFN. In contrast, anti-chick IFN serum did not inhibit the ageing effect, i.e. the enhanced production of IFN upon induction after a prolonged preincubation of CE cells. This result indicates that the occurrence of the ageing effect is not mediated by the IFN system which had been expressed spontaneously.
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000375:Aging / Q000187:drug effects; D000818:Animals; D002642:Chick Embryo / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007372:Interferons / Q000096:biosynthesis*
D007106:Immune Sera; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase