title: Helper activity for antibody synthesis encoded by mRNA extracted from human peripheral blood mononuclear cells.
abstract: Translation products in Xenopus laevis of mRNA from human peripheral blood mononuclear cells were tested for their capacity to replace T cells in the anti-SRBC response of nude spleen cells. When the starting material came from PHA or FCS-stimulated lymphocytes, the translated lymphokines, displaying such a B cell helper activity were found to be encoded by mRNA sedimenting at 6-7S and 13S on a sucrose density gradient. 6-7S mRNA from control, non-stimulated lymphocytes was also able to code for B cell helper activity. Thorough T-cell depletion of mouse responder cell populations left unchanged the activity of 6-7S mRNA products while preventing that of 13S products. The latter were found to contain IL-2, which suggests that their action on B cells was indirect, mediated by T-cell stimulation.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics*; D000917:Antibody Formation; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014981:Xenopus
chemical_list: D000906:Antibodies; D007376:Interleukin-2; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA