Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9.
The phenol-phase soluble cellular lipopolysaccharide isolated by the phenol/water extraction method from Yersinia enterocolitica serotype O:9 cells was shown by hydrolytic, periodate oxidation, methylation and nuclear magnetic resonance studies to be an S-type lipopolysaccharide with a linear O-antigenic polysaccharide of 1,2-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl units. The serological cross-reactivity between Y. enterocolitica serotype O:9 and the lipopolysaccharides of Vibrio cholerae and Brucella species can now be related to the presence of N-acylated 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl residues in their respective O-antigenic chains.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D007120:Immunochemistry; D008050:Lipid A; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D019081:O Antigens; D019800:Phenol; D010636:Phenols; D012703:Serotyping; D012995:Solubility; D015007:Yersinia / Q000276:immunology*
D000942:Antigens, Bacterial; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D010636:Phenols; D019800:Phenol