A synthetic peptide induces a new anti-dextran idiotype.
We have synthesized the JH1 peptide and coupled it to keyhole limpet haemocyanin for use as an immunogen. A serum from an animal hyperimmunized with this immunogen demonstrated binding to both peptide and native immunoglobulin. Furthermore, this serum seems to define an idiotypic determinant nearly unique to alpha (1 ---- 3) dextran-binding immunoglobulins. Synthetic peptide immunogens may be a useful tool in probing the molecular basis of idiotypy.
D000595:Amino Acid Sequence; D000818:Animals; D003911:Dextrans / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007133:Immunoglobulin Joining Region / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology; D051379:Mice; D011817:Rabbits
D003911:Dextrans; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region