Cellular expression of murine leukemia virus gp70-related antigen on thymocytes of uninfected mice correlates with Fv-4 gene-controlled resistance to Friend leukemia virus infection.
Alleles at the Fv-4 locus have been shown to determine susceptibility (Fv-4s) and resistance (Fv-4r) to infection with ecotropic murine leukemia viruses (MuLV). The resistance is dominant in heterozygous mice. BALB/c mice are Fv-4ss and a semicongenic line BALB/c-Fv-4wr, homozygous for Fv-4r allele and called C4W, is being developed. Extracts of 125I-labeled thymocytes from BALB/c and C4W mice were precipitated by a series of antisera against MuLV and the precipitates examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Thymocytes from C4W mice expressed 80K and 73K glycoproteins related to the gp70 of MuLV, while only the 73K glycoprotein was occasionally detectable on BALB/c thymocytes. Genetic studies showed that a locus controlling expression of the 80K protein was dominant and associated with the resistant allele at Fv-4. This membrane gp70 may be important in the Fv-4-mediated resistance to exogenous virus infection.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D000939:Epitopes; D005622:Friend murine leukemia virus; D005796:Genes; D007113:Immunity, Innate; D009052:Leukemia Virus, Murine / Q000276:immunology*; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*
D000954:Antigens, Surface; D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; D014764:Viral Proteins