Absence of procollagen alpha 2(I) mRNA in chemically transformed rat liver epithelial cells.
The W8 cell line, a chemically transformed variant of K16 rat liver epithelial cells, does not produce a normal type I collagen but instead secretes alpha 1(I) trimer and no alpha 2(I) chains (Smith, B.D., and Niles, R. (1980) Biochemistry 19, 1820-1825). Analysis of total cellular RNA from the K16 and W8 cells by cell-free translation in a rabbit reticulocyte lysate demonstrates the specific absence of translatable pro-alpha 2(I) collagen mRNA. No alpha 2(I) collagen mRNA sequences were detected by Northern hybridization analysis using cloned rat alpha 2(I) cDNAs as a probe. The total amount of alpha 1(I) collagen mRNAs, however, was the same in both cell types, suggesting a lack of coordinate control of alpha 1(I) and alpha 2(I) mRNAs in the transformed cells.
D000099:Acetoxyacetylaminofluorene; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D011347:Procollagen / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism
D011347:Procollagen; D012333:RNA, Messenger; D011061:Poly A; D000099:Acetoxyacetylaminofluorene; D012313:RNA; D004247:DNA