Forssman antigenicity of chemically synthesized globopentaose.
The Forssman antigenicity of a chemically synthesized globopentaose was studied. Globopentaose at 40 ng showed strong inhibitory activity for the formation of a precipitin line between globopentaosylceramide (Forssman glycolipid) and anti-Forssman rabbit antiserum, while much more pentasaccharide (7 and 100 micrograms, respectively) was required to inhibit a 50% quantitative precipitin reaction and a hemolysis reaction. An immune complex of the 3H-labeled globopentaose with anti-Forssman antibody was hardly formed. Thus, the chemically synthesized globopentaose possesses the same antigenic specificity as globopentaosylceramide but it is difficult to achieve a stable complex with Forssman antibody.
D000818:Animals; D015478:Antigens, Heterophile; D000939:Epitopes; D005577:Forssman Antigen; D005915:Globosides / Q000138:chemical synthesis* / Q000276:immunology; D006028:Glycosphingolipids / Q000138:chemical synthesis*; D007074:Immunoglobulin G / Q000276:immunology; D011233:Precipitin Tests; D012756:Sheep
D015478:Antigens, Heterophile; D000939:Epitopes; D005915:Globosides; D006028:Glycosphingolipids; D007074:Immunoglobulin G; C010163:Forssman glycolipid; D005577:Forssman Antigen