Binding of [3H]substance P to putative substance P receptors in rat brain membranes.
[3H]Substance P was used to label putative substance P receptors in rat brain membranes. [3H]Substance P binding was saturable, reversible, and displaceable by non-radioactive substance P. Association, dissociation and saturation experiments suggest a single site interaction of [3H]substance P to its binding site (k-1/k+1 = 0.56 nM; KD = 0.9 nM, Bmax 86.5 fmol/mg protein.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018040:Receptors, Neurokinin-1; D013373:Substance P / Q000378:metabolism*
D011956:Receptors, Cell Surface; D018040:Receptors, Neurokinin-1; D013373:Substance P