A monoclonal antibody recognizes an epitope common to an avian-specific nuclear antigen and to cytokeratins.
X3, a monoclonal antibody of unusual specificity, is described. This antibody reacts with one or more cytokeratin polypeptides and also reacts with an avian (chicken, quail) nuclear antigen that appears to be present in all cell types (chicken) tested, although with variable staining pattern and intensity. This antigen is distinct from the cytokeratins but does have an epitope in common with this class of proteins. It disappears from the nucleus during the early stages of cell division and reappears during anaphase as a granular cytoplasmic structure. In late telophase the antigen is relocated in the nucleus. This antigen, which we have designated as avian-specific nuclear antigen ( AVNA ), is not associated with chromatin or ribonucleoproteins. From immunoblotting experiments on chicken fibroblast nuclei, AVNA is probably a complex composed of one or several polypeptides, one of which has a molecular weight of approximately 60 kD. The proteins were identified as nuclear matrix proteins rather than pore complex-lamina proteins by immunoblotting experiments on the purified nuclear matrix of chicken erythrocytes. The major polypeptide had a molecular weight of 60 kD and the minor polypeptide a molecular weight of 69 kD.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D034961:Antigens, Nuclear; D002478:Cells, Cultured; D002642:Chick Embryo; D006224:Cricetinae; D003599:Cytoskeleton / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins / Q000276:immunology*; D051379:Mice; D009698:Nucleoproteins / Q000276:immunology*; D011784:Quail / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D000939:Epitopes; D009698:Nucleoproteins; D007633:Keratins