The effect of isobutylmethylxanthine on the photoresponse and phosphorylation of phosphatidylinositol in octopus retina.
The effect of isobutylmethylxanthine on the photoresponse and the phosphorylation of phosphatidylinositol was studied. The recovery of photoresponse after a bright flash was greatly altered by the addition of isobutylmethylxanthine. Phosphatidylinositol phosphorylation, assessed by the incorporation of label from [gamma-32P]ATP, was influenced by isobutylmethylxanthine. Incorporation of 32P was decreased into diphosphoinositide and triphosphoinositide, and increased into phosphatidic acid. These changes were interpreted by introducing a parameter, phi, calcium charge of the plasma membrane.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006152:Cyclic GMP / Q000494:pharmacology; D003624:Darkness; D007700:Kinetics; D008027:Light; D049831:Octopodiformes; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D010775:Photic Stimulation; D010786:Photoreceptor Cells / Q000187:drug effects / Q000502:physiology*; D012160:Retina / Q000187:drug effects / Q000502:physiology*; D013806:Theophylline / Q000031:analogs & derivatives*
D010716:Phosphatidylinositols; D000255:Adenosine Triphosphate; D013806:Theophylline; D006152:Cyclic GMP; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine