title: Histochemical investigation of criteria for the distinction between monoamine oxidase A and B in various species.
abstract: The superior cervical ganglion (SCG), pineal body (PB), and liver (L) of the rat, rabbit and cat were stained for monoamine oxidase (MAO) A and B by the tetranitro blue tetrazolium (TNBT) and coupled peroxidase ( PerOx ) methods, using 5-hydroxytryptamine (5HT), tryptamine ( Tryp ), tyramine (Tyr), and benzylamine (Bz) as substrates, and clorgyline (Cl) and deprenyl (Dep), both at 10(-7) M, as selective inhibitors. The nodose ganglion (NG) and dorsal root ganglion (DRG) of the rabbit and cat were also studied. The results with rat tissues were consistent with published quantitative findings (SCG, MAO-A much greater than B; PB, MAO-A less than or equal to B; L, MAO-A = B). In the rabbit, the findings with the SCG were similar; the MAO activities of the PB were relatively resistant to both inhibitors; the MAO of the liver required 10(-4) M concentrations of both inhibitors to produce near total inhibition, suggesting that the liver contains an MAO distinct from MAO A and B. All cat tissues examined appeared to contain almost exclusively MAO-B. In this species 5HT, which is generally considered a selective substrate for MAO-A, was oxidized by MAO-B. The findings indicate that criteria for MAO-A, -B, and other subgroups must be defined for each species and tissue.
mesh_terms: D000818:Animals; D002415:Cats; D003010:Clorgyline / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000201:enzymology*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008099:Liver / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000032:analysis*; D008996:Monoamine Oxidase Inhibitors / Q000032:analysis; D010870:Pineal Gland / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D012642:Selegiline / Q000494:pharmacology; D013045:Species Specificity; D013194:Staining and Labeling; D013778:Tetrazolium Salts
chemical_list: D008996:Monoamine Oxidase Inhibitors; D013778:Tetrazolium Salts; C028130:tetranitrotetrazolium blue; D012642:Selegiline; D006735:Horseradish Peroxidase; D008995:Monoamine Oxidase; D003010:Clorgyline