Reduced fading of Fast Blue fluorescence in the brain of the guinea-pig by treatment with sodium-nitroprusside.
Fading of the emitted fluorescent light of the retrogradely transported neuronal tracer Fast Blue is reduced considerably by treating sections for 20 min with sodium-nitroprusside (0.4% in 0.1 M acetate buffer; pH 3.3) and mounting sections from acetate buffer. Mounting from phosphate buffer abolishes the antifading effect of sodium-nitroprusside.
D000578:Amidines; D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping / Q000379:methods*; D005292:Ferricyanides; D006168:Guinea Pigs; D008856:Microscopy, Fluorescence; D009599:Nitroprusside; D013194:Staining and Labeling / Q000379:methods*
D000578:Amidines; D005292:Ferricyanides; C031455:diamidino compound 253-50; D009599:Nitroprusside