title: Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.
abstract: The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.
mesh_terms: D000260:Adenoviruses, Human / Q000378:metabolism; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D009690:Nucleic Acid Conformation; D009694:Nucleic Acid Precursors / Q000032:analysis / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism*; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism*
chemical_list: D009694:Nucleic Acid Precursors; D009843:Oligoribonucleotides; D010710:Phosphates; D012322:RNA Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D012313:RNA