Extraction of osmium-containing lipids by section staining for TEM.
Postfixation with osmium-ferrocyanide or OSO4 renders lipid droplets in rat liver and kidney homogeneously electron dense without additional section staining. In sections of the same block that have been single stained by uranyl acetate or lead citrate, lipid droplets show a more electron translucent center surrounded by a dense rim. In sections double stained with uranyl acetate and lead citrate, lipid droplets frequently appear as empty vacuoles, from which the electron dense content has been completely extracted.
D000818:Animals; D002951:Citrates; D019343:Citric Acid; D007668:Kidney / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D009992:Osmium / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D014501:Uranium
D002951:Citrates; D008055:Lipids; D009942:Organometallic Compounds; C005460:uranyl acetate; D019343:Citric Acid; D009992:Osmium; D014501:Uranium