Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin.
Two monoclonal mouse antibodies against human plasma fibronectin were compared in their reactivity for proteolytic fragments of the antigen by enzyme immunoassay and immunoblotting. These antibodies were shown to react with two different structures within a short segment (about 30 kDa) located about one-third away from the C-terminus of the fibronectin chains.
D000818:Animals; D000911:Antibodies, Monoclonal; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes; D005260:Female; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005353:Fibronectins; D010446:Peptide Fragments