[Cloning and expression of the genetic determinants encoding for the serological variants of the adhesive factor K 88 carried by enterotoxinogenic Escherichia coli strains of porcine origin].
The existence of homologous sequences on plasmids carried by 3 enterotoxinogenic strains of Escherichia coli of porcine origin, and encoding for adhesive factors of different antigenic specificities: K 88 ab, K 88 ac, K 88 ad; has been demonstrated by DNA-DNA hybridization. The use as probe of a DNA fragment carrying a piece of structural genes of the adhesive factor K 88 ab taken out of a recombinant plasmid expressing this factor, has allowed us to localize the genetic determinants which encode for the adhesive factors K 88 ac and K 88 ad. Cloned in the plasmid pBR 322, these determinants have allowed us to obtain the expression, by the host bacteria of the corresponding adhesive factors.
D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D005796:Genes; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013552:Swine / Q000382:microbiology*
D004269:DNA, Bacterial; D004768:Enterotoxins; D000939:Epitopes
Clonage et expression des déterminants génétiques codant pour les sous-types du facteur d'attachement K 88 porté par les souches d'Escherichia coli entérotoxinogènes d'origine porcine.