Acceptor-suppressor T cell hybridoma with a receptor recognizing antigen-specific suppressor factor.
An acceptor hybridoma with a receptor that recognizes the keyhole limpet hemocyanin (KLH)-specific suppressor T cell factor (KLH-TsF) was established after the fusion of C57BL/6 splenic T cells enriched with KLH-coated petri dishes. The cloned hybridoma (34S-281) could be specifically activated by stimulation with the conventional KLH-TsF or monoclonal KLH-TsF from three different hybridomas in the absence of the relevant antigen (KLH) and it started to produce another factor that suppresses the antibody response against DNP-KLH in a KLH-specific fashion. The KLH specificity of the TsF was required for activation. The new factor was found not to bind the KLH but to be absorbed with the KLH-TsF-producing hybridoma. It is thus strongly suggested that the acceptor site has a complementary structure (antiidiotype) for the KLH-TsF. Moreover, the idiotypic determinant on KLH-TsF was found to have a structure similar to that on some of the anti-KLH antibodies, since the acceptor hybridoma was specifically killed by the conventional anti-KLH antibodies and complement. Drawing on the above results, the idiotype-antiidiotype network in the conventional antigen system is discussed.
D000818:Animals; D000939:Epitopes; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011971:Receptors, Immunologic / Q000276:immunology*; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D008222:Lymphokines; D011971:Receptors, Immunologic; D013491:Suppressor Factors, Immunologic; D006433:Hemocyanins