Purification and some properties of a plasma protein which reacts with an antiserum against urinary trypsin inhibitor-1.
A plasma protein which forms a precipitation line with antiserum against urinary trypsin inhibitor was purified by ammonium sulphate fractionation, anti-urinary trypsin inhibitor rabbit IgG antibody coupled sepharose immunoadsorbent column chromatography and polyacrylamide-gel disc electrophoresis. By these procedures, 0.14 mg of purified material was obtained from 20 ml of plasma. It was homogeneous as ascertained by sodium dodecyl sulphate polyacrylamide-gel disc electrophoresis and had an apparent molecular weight of 90,000. It was found that this inhibitor was immunologically distinct from inter-alpha-trypsin inhibitor.
D000510:Alpha-Globulins / Q000276:immunology* / Q000652:urine; D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity; D006801:Humans; D007106:Immune Sera; D007163:Immunosorbent Techniques; D012712:Serum Globulins / Q000276:immunology / Q000302:isolation & purification*; D014361:Trypsin Inhibitors / Q000276:immunology*
D000510:Alpha-Globulins; D007106:Immune Sera; D012712:Serum Globulins; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor