Characterization of virus-like particles from a psoriatic patient with respect to the possible presence of particle-associated RNA and RNA-directed DNA polymerase.
Subcellular particles resembling retroviruses with respect to their morphology, density, and protein composition, and which are present in the urine of a psoriatic patient have been analysed for the presence of high-molecular-weight polyadenylated RNA and RNA-directed DNA polymerase activity. Neither RNA-directed DNA polymerase activity nor high-molecular-weight polyadenylated RNA was detected in preparations containing the purified virus-like particles. This was the case even when the amount of particles analysed exceeded by a factor of 50 or more the amount of avian myeloblastosis virus in which both polymerase activity and RNA were detected.
D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis*; D011565:Psoriasis / Q000382:microbiology*; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger; D012367:RNA, Viral / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D014771:Virion / Q000032:analysis* / Q000201:enzymology / Q000302:isolation & purification
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA; D012194:RNA-Directed DNA Polymerase