Surface antigens of murine hemopoietic stem cells. IV. Characteristics of antisera exhibiting cell-lineage specificity, and differentiating ability of stem cells surviving treatment with antisera against cell-lineage antigens.
Hemopoietic stem cells express cell-lineage antigens in common with platelets, thymocytes and macrophages. Further evidence for the cell-lineage antigen model is presented. Erythrocyte-absorbed antiserum against purified mouse neutrophils cross-reacts with the hemopoietic stem cell in a lineage-specific manner. Thus, most of the anti-stem cell activity is absorbed out with neutrophils but not by other non-granulocytic hemopoietic cells. Of the four antisera tested for anti-stem cell activity, antiplatelet serum showed the highest degree of cell-lineage specificity (96% of the anti-stem cell activity satisfied cell-lineage criteria). To test predictions of the cell-lineage model of hemopoietic cell differentiation concerning the differentiating potential of stem cells selected for low concentration of cell-lineage antigen, bone marrow cells were treated with antiplatelet serum at a predetermined concentration required to produce 75-85% loss of spleen colonies. Stem cells surviving this treatment showed normal ability to form megakaryocytes and platelets. The results are consistent with postulates of the cell-lineage model.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D000939:Epitopes / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008954:Models, Biological; D009504:Neutrophils / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D000939:Epitopes; D007106:Immune Sera