Flow microfluorometric analysis of living spermatozoa stained with Hoechst 33342.
Bovine spermatozoa were stained with Hoechst 33342. The fluorescence distribution of stained spermatozoa was complex. Non-motile spermatozoa displayed a higher fluorescence than did motile spermatozoa. The fluorescence profile of the motile spermatozoa was bimodal. Sort and reanalysis, and orientation experiments suggested that there are two distinct populations of motile spermatozoa.
D000818:Animals; D001562:Benzimidazoles; D002417:Cattle; D002645:Chickens; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling
D001562:Benzimidazoles; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride