Cytofluorometric DNA base determination for the investigation of heterochromatin and heterochromatin amplification.
The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band areas of the chromosomes of this species. In Sinapis alba three different heterochromatin types, with regard to base content, can be discerned. The heterochromatin amplification observed in the polyploid nuclei of Sinapis roots and hypocotyl involves either all three heterochromatin types, with little resulting change of the total nuclear base content, or only one or the other of them, with a measurable shift of the base content.
D000225:Adenine / Q000032:analysis; D001482:Base Composition; D002843:Chromatin / Q000032:analysis*; D014128:Chromomycin A3; D002871:Chromosome Banding; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis*; D022041:Euchromatin; D006147:Guanine / Q000032:analysis; D006570:Heterochromatin / Q000032:analysis* / Q000096:biosynthesis; D007211:Indoles; D010944:Plants / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013941:Thymine / Q000032:analysis
D002843:Chromatin; D022041:Euchromatin; D006570:Heterochromatin; D007211:Indoles; C007293:DAPI; D006147:Guanine; D003596:Cytosine; D004247:DNA; D014128:Chromomycin A3; D000225:Adenine; D013941:Thymine