[Synthesis of RNA and DNA in isolated nuclei of Colpoda cucullus (Protozoi Ciliati)].
The ciliated protozoan Colpoda cucullus has been cultivated at 27 degrees C with gentle shaking in a baked lettuce infusion supplemented with Klebsiella suspensions. Under these conditions cells had a mean generation time of about 7 hours and could attain densities up to 20,000/ml and 45,000/ml in the log and stationary phase of growth, respectively. Nuclear preparations obtained from exponentially growing cells by the gum arabic-octanol method showed a satisfactory degree of purity and integrity. They consisted primarily of the large macronuclei attached to which the small micronuclei were sometimes visible. Upon incubation at 27 degrees C in conventional reaction mixtures nuclear preparations actively incorporated 3H-UTP and 3H-dTTP into acid-insoluble material. alpha-amanitin caused a 50% inhibition of RNA synthesis whereas aphidicolin did not affect at all DNA synthesis.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D016590:Aphidicolin; D002467:Cell Nucleus / Q000378:metabolism; D016798:Ciliophora / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D013942:Thymine Nucleotides / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism
D000546:Amanitins; D004224:Diterpenes; D013942:Thymine Nucleotides; D016590:Aphidicolin; D012313:RNA; C024157:thymidine 5'-triphosphate; D014544:Uridine Triphosphate
Sintesi di RNA e DNA nei nuclei isolati di Colpoda cucullus (Protozoi Ciliati).