[Comparison of counterimmunoelectrophoresis with standard serological tests in the diagnosis of ovine brucellosis].
Sera from 30 sheep experimentally infected and from 126 others with chronic brucellosis were comparatively studied by serum agglutination (SA), complement fixation (CF), rose bengal (RB), and counter-immunoelectrophoresis (CIEP) test. Standard serological tests, particularly SA and RB, essentially detect antibodies against cell-wall fractions of Brucella (LPS), whereas CIEP responds to intracellular antigens. Antibodies involved in this test appear late but remain a long time in the sera. CIEP is therefore recommended for screening of animals with chronic brucellosis in which SA and often CF and RB tests too negative.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002003:Brucella abortus / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D003168:Complement Fixation Tests / Q000662:veterinary; D003378:Counterimmunoelectrophoresis; D000939:Epitopes; D006386:Hemagglutination Tests / Q000662:veterinary; D007122:Immunoelectrophoresis; D012395:Rose Bengal; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000939:Epitopes; D012395:Rose Bengal
Comparaison entre électrosynérèse et épreuves sérologiques classiques dans le diagnostic de la brucellose ovine.