In vitro cartilage differentiation: modification of division cycle and effect of some polyanions.
A modification of division cell cycle under conditions of in vitro cartilage differentiation could be demonstrated. The frequency distribution of histograms showed that the G1 cells represented 50% of total cells number at zero time (i.e. in suspension before inoculation), 69% on day 2 and about 90% in following period of days 4, 6 and 14. Dextran sulfate and hyaluronic acid produced a conspicuous inhibition and the chondroitin sulfate as well as heparin exerted a moderate inhibiting effect on the cell proliferation.
D000818:Animals; D002356:Cartilage / Q000032:analysis / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D003911:Dextrans / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects; D013997:Time Factors
D003911:Dextrans; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates; D004247:DNA