dCMP-aminohydrolase activity during early sea urchin development. An example of negative enzyme control during embryogenesis.
In sea urchin, unfertilized eggs have a very high level of dCMP-aminohydrolase (dCMPase) activity, which decreases gradually and at the pluteus stage it is only about a quarter of that found in the unfertilized egg. But in abnormal embryos and in disaggregated cells from embryos, no decrease in the dCMPase activity takes place. To understand the control mechanism involved in this enzyme activity during development, we have analyzed the effect of various drugs which interfere with information transfer, such as actinomycin C, puromycin, 5-azacytidine, 2-thio-uracil and p-fluoro-DL-phenylalanine on dCMPase activity in embryos of Paracentrotus lividus and Sphaerechinus granularis. Among these drugs only actinomycin induces a remarkable increase of the dCMPase activity in embryos with respect to unfertilized eggs. Puromycin has a differential and dose-dependent effect. Other drugs, although they affect normal development and macromolecular synthesis, do not significantly alter the dCMPase activity. On the basis of these results we suggest the presence of a repressor mechanism in the control of dCMP-aminohydrolase level during early embryogenesis of sea urchin.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D003844:DCMP Deaminase / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D009709:Nucleotide Deaminases / Q000378:metabolism*; D010063:Ovum / Q000201:enzymology; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012617:Sea Urchins; D013889:Thiouracil / Q000494:pharmacology; D010135:p-Fluorophenylalanine / Q000494:pharmacology
C030013:cactinomycin; D003609:Dactinomycin; D011691:Puromycin; D013889:Thiouracil; D010135:p-Fluorophenylalanine; D012313:RNA; D004247:DNA; D009709:Nucleotide Deaminases; D003844:DCMP Deaminase; D001374:Azacitidine