Growth of antigen specific, HLA restricted T lymphocyte clones from cerebrospinal fluid.
Analysis of the local cell-mediated immunity within the central nervous system is limited by the small amount of material available. In the present report we describe that T lymphocyte clones can be expanded directly from the cerebrospinal fluid (CSF) using T cell growth factor and irradiated feeder cells. Without in vitro restimulation such clones can reproducibly be generated from fresh or cryopreserved CSF lymphocytes. From a patient with tuberculous meningitis, T lymphocyte clones were obtained that showed tuberculin (PPD) specific proliferative responses restricted by a single HLA-DR antigen. One of these clones was restricted by an antigen different from the serologically defined HLA-DR since this clone recognized PPD only on autologous but not on HLA-DR matched monocytes. These experiments show that T lymphocytes involved in the in situ immune response can be cloned directly from the site of inflammation.
D002478:Cells, Cultured; D002555:Cerebrospinal Fluid / Q000166:cytology* / Q000276:immunology; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008582:Meningitis, Aseptic / Q000276:immunology; D008875:Middle Aged; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D014390:Tuberculosis, Meningeal / Q000276:immunology
D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010835:Phytohemagglutinins; D014373:Tuberculin