[QFH banding and DNA distribution in the macrochromosomes of the chicken Gallus domesticus].
Chromosomes of Gallus domesticus were stained with rivanolum-SO2 (fluorescence Feulgen reaction) and by Hoechst 33258. Fluorescence photography was performed on a 35 mm film KN-3(KN-3) "Svema". The negatives were analyzed with the microdensitometer. The Feulgen (Fr--) and the Hoechst 3358 (H--) densitometric profiles of chromosomes showed light and dark segments along the metaphase chromosomes. The amount of DNA, as determined by the fluorescence Feulgen reaction, is not constant along the chromosome arms. Consequently, the base composition is not the only factor influencing the fluorescence of Hoechst 33258 along the chromosomes. The comparative analysis of densitometric profiles of the Hoechst 33258 and rivanolum-SO2 stained chromosomes shows that the Iq telomere band consists of GC-rich DNA (about 70% GC). The variation of DNA contents along the metaphase chromosome arms can be realized at both chromoneme and/or subchromoneme levels.
D000818:Animals; D006690:Bisbenzimidazole; D001853:Bone Marrow / Q000648:ultrastructure; D002645:Chickens / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods; D002875:Chromosomes / Q000032:analysis / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D003720:Densitometry; D004975:Ethacridine / Q000031:analogs & derivatives
D004247:DNA; D006690:Bisbenzimidazole; D004975:Ethacridine
QFH-ischerchennost' i raspredelenie DNK v makrokhromosomakh kuritsy Gallus domesticus.