Transformation of LMTK- cells with purified HLA class I gene. VI. Serological characterization of HLA-B7 and AW24 molecules.
Serological characterization of HLA-B7 and HLA-AW24 class I molecules following transfection of murine LMTK- cells with purified HLA class I genes was performed using human alloantisera. Induction by murine alpha interferon of the expression of class I molecules was required to obtain unambiguous identification of these molecules which appear serologically identical to the HLA-B7 and HLA-AW24 molecules expressed at the surface of human peripheral blood lymphocytes of 20 unrelated individuals. Analysis of the transformed cells with 8 different anti-HLA class I monoclonal antibodies results in the definition of 3 separate clusters of antigenic determinants shared by all HLA class I molecules. These studies further suggest the existence of locus-specific serological reactivities associated either with the HLA-A or with the HLA-B and C gene products.
D000818:Animals; D000936:Antigen-Antibody Complex; D004262:DNA Restriction Enzymes; D000939:Epitopes / Q000032:analysis; D005796:Genes; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens; D059871:HLA-A24 Antigen; D015793:HLA-B7 Antigen; D006801:Humans; D007739:L Cells / Q000201:enzymology; D008285:Major Histocompatibility Complex; D051379:Mice; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
D000936:Antigen-Antibody Complex; D000939:Epitopes; D006183:H-2 Antigens; D006680:HLA Antigens; D015234:HLA-A Antigens; D059871:HLA-A24 Antigen; D015793:HLA-B7 Antigen; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes