Establishment of two chick embryo fibroblastic cell lines.
Chicken helper factor (chf)-negative chick embryo cells (CEC) were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), cultured at 41 degrees in a CO2-incubator and are currently in the 400th passage (over 4 years). They were designated as CHCC-OU1. They pile up, and form colonies in soft agar, but do not produce tumors either in the syngeneic chicken or in nude mice. Chf-negative CEC without MNNG treatment were maintained in the same way and are currently in the 350th passage (over 3.5 years). They were designated as SPCC-OU1. They appear normal and form no colony in soft agar. Both cell lines produce avian endogenous leukosis virus of subgroup E.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo / Q000166:cytology*; D002875:Chromosomes; D005347:Fibroblasts / Q000166:cytology*; D008769:Methylnitronitrosoguanidine; D010641:Phenotype; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014762:Viral Interference; D014776:Virus Cultivation
D008769:Methylnitronitrosoguanidine; D012194:RNA-Directed DNA Polymerase