The production of ceroid by mouse peritoneal macrophages in vitro.
Murine resident peritoneal macrophages accumulated lipid droplets and subsequently insoluble, ceroid-like material when cultured in vitro in a medium containing 33% fetal calf serum. At least some of this insoluble lipid was membrane-bound and by light microscopy it often appeared as 'rings' with a hollow centre. It is suggested that the production of ceroid may be the consequence of the uptake of lipids from the extracellular medium and the activity of the macrophage's membrane-bound oxidative microbicidal mechanisms. The results indicate that macrophages are capable of rendering lipids insoluble, supporting the suggestion that this might occur in the atherosclerotic plaque.
D000818:Animals; D001202:Ascitic Fluid; D002478:Cells, Cultured; D002566:Ceroid / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010860:Pigments, Biological / Q000096:biosynthesis*; D013194:Staining and Labeling; D013997:Time Factors
D002566:Ceroid; D010860:Pigments, Biological