title: Ultrastructural localization of cellulase in Trichoderma reesei using immunocytochemistry and enzyme cytochemistry.
abstract: Two components of the cellulase complex (E.C. 3.2.1.4) of the fungus Trichoderma reesei were localized at the ultrastructural level. Immunocytochemistry and enzyme cytochemistry demonstrated that cellobiohydrolase and beta-1,4 glucanase were localized within cisternae of endoplasmic reticulum and within membrane complexes of cellulose-grown hyphae. Both enzymes were also present in the culture medium. Glucose-grown control hyphae lacked enzyme-specific staining, and no enzyme activity was detected in the growth medium.
mesh_terms: D002480:Cellulase / Q000032:analysis* / Q000302:isolation & purification; D043366:Cellulose 1,4-beta-Cellobiosidase; D003470:Culture Media / Q000032:analysis; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000032:analysis; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000201:enzymology; D008854:Microscopy, Electron; D003904:Mitosporic Fungi / Q000201:enzymology*; D013194:Staining and Labeling; D014242:Trichoderma / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D006026:Glycoside Hydrolases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase