Antigen-induced neutrophil chemotactic activity from sensitized lung.
We have used the model of ovalbumin-sensitized guinea pigs to define the source of antigen-derived, high-molecular-weight neutrophil chemotactic activity (NCA). Incubating sensitized lung fragments with specific antigen (ovalbumin) but not irrelevant antigen (bovine serum albumin) or buffer resulted in the release of high-molecular-weight NCA and histamine, suggesting a lung mast cell origin for NCA. High-molecular-weight NCA accounted for greater than 90% of the NCA observed after antigen-lung incubation. The importance of high-molecular-weight NCA in recruitment of neutrophilic leukocytes to the site of allergic inflammatory reactions is emphasized.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D002465:Cell Movement; D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis*; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis; D002850:Chromatography, Gel; D006168:Guinea Pigs; D006636:Histamine Release; D016209:Interleukin-8; D008168:Lung / Q000276:immunology*; D008970:Molecular Weight; D009504:Neutrophils / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology
D000941:Antigens; D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D016209:Interleukin-8; D010047:Ovalbumin