title: Nucleotide sequence of nuclear 5.4 S RNA of mouse cells.
abstract: The nucleotide sequence of nuclear 5.4 S RNA, a new species of small nuclear RNA (snRNA) of mouse cells, was determined. The 5.4 S RNA consists of 138 nucleotide residues containing 1 mol each of 2,2,7- trimethylguanosine (m3(2,2,7) G), 2'-O-methyladenosine (Am), 2'-O-methyluridine (Um) and pseudouridine as modified nucleosides. This RNA has a cap structure, m3(2,2,7) ++GpppAm -, at its 5'-terminus and sequences complementary to the terminal consensus sequences of introns. The sequence complementary to the 5'-splice junction, A-U-C-C-psi-U-A-C-C-U-G, is very similar to the 5'-terminal sequence of U1 RNA.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D007942:Leukemia, Experimental; D051379:Mice; D009690:Nucleic Acid Conformation; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012315:RNA Caps / Q000302:isolation & purification; D012326:RNA Splicing; D012342:RNA, Small Nuclear; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic
chemical_list: D011687:Purines; D011743:Pyrimidines; D012315:RNA Caps; D012342:RNA, Small Nuclear; D012313:RNA; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic