[Identification of histone mRNA and isolation of a DNA fraction enriched with Physarum polycephalum histone genes].
The 7-14S poly A- RNA isolated from the S-state polysomes of the macroplasmodium P. polycephalum reveals the properties of histone mRNA. In the wheat germ cell-free system this RNA is translated into the polypeptides with the same electrophoretic mobility as core histones of P. polycephalum. Using centrifugation in a CsCl density gradient with actinomycin D, a DNA fraction was isolated which hybridizes with cDNA synthesized at 7-14S poly A- RNA by reverse transcription with random priming. The DNA fraction formed can be used for cloning of P. polycephalum histone genes.
D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D006657:Histones / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D010804:Physarum / Q000235:genetics*; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D014908:Triticum / Q000378:metabolism
D004271:DNA, Fungal; D006657:Histones; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA
Identifikatsiia gistonovykh mRNK i vydelenie fraktsii DNK, obogashchennoĭ gistonovymi genami Physarum polycephalum.