A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I.
A monoclonal mouse IgG1 antibody was produced against the aminopropeptide of dermatosparactic sheep procollagen type I by using the hybridoma technique. Radioimmunoassays demonstrated an apparent affinity constant of 10(8) l X mol-1. The antibody reacted with a 19-amino-acid-long sequence spanning the procollagen N-proteinase cleavage site with stronger binding to structures contributed by the aminopropeptide. The antibody showed strong cross-reactions with similar antigens of bovine, human or chick origin but failed to react with the aminopropeptide of procollagen type III. Incubation of chick or sheep procollagen type I with stoichiometric amounts of antibody blocked the release from procollagen molecules of the aminopropeptide by procollagen N-proteinase. Thus, this antibody seems useful for studying various biological problems encountered in the conversion of procollagen.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D001665:Binding Sites; D002417:Cattle; D002645:Chickens; D000939:Epitopes / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D011347:Procollagen / Q000276:immunology*; D011817:Rabbits; D012756:Sheep
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D011347:Procollagen