Monoclonal antibodies to thymosin alpha 1.
Sixteen monoclonal antibodies specific for thymosin alpha 1 [Low et al., J. biol. Chem. 254, 981-986 (1979)] obtained from two fusions with the spleens of three mice [Stähli et al., Meth. Enzym. 92, 26-36 (1982b)] all react with an epitope in the C-terminal half of thymosin alpha 1. Human and foetal calf serum contain substances which cross-react with this epitope. A simple procedure to selectively remove the cross-reactive material and a sensitive RIA are described.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology*; D000918:Antibody Specificity; D001769:Blood; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000276:immunology; D051379:Mice; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D013947:Thymosin; D000077596:Thymalfasin