In vivo and in vitro binding of alpha- and gamma-hexachlorocyclohexane to mouse liver macromolecules.
alpha-Hexachlorocyclohexane (alpha-HCH) but not gamma-HCH, produces a strong neoplastic response in HPB strain mouse liver. In vivo and in vitro binding studies with 14C-labelled HCH isomers showed no preferential binding of the alpha-HCH isomer to protein or DNA. Moreover, the binding of both isomers to DNA in vivo or in vitro occurred only at very low levels, a result consistent with the lack of mutagenic activity associated with these compounds. The results suggest that the neoplastic response observed with alpha-HCH results from a non-genotoxic mechanism.
D000818:Animals; D004247:DNA / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D066298:In Vitro Techniques; D046911:Macromolecular Substances / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D011485:Protein Binding; D012313:RNA / Q000378:metabolism
D046911:Macromolecular Substances; C040534:alpha-hexachlorocyclohexane; D001556:Hexachlorocyclohexane; D012313:RNA; D004247:DNA; D010634:Phenobarbital