Anti-IgE-induced histamine release from human basophilic leukocytes: inhibition depends on nature and concentration of inhibitor and secretagogue.
Histamine release from human basophilic leukocytes was induced by increasing concentrations of anti-IgE in the presence of various concentrations of 2-deoxyglucose (2-DOG), the histamine H2-receptor agonist dimaprit, theophylline, or enprofylline (a new antiasthmatic xanthine derivative). The results show that the degree of inhibition produced by each agent differed with the concentration of anti-IgE used and with the nature and concentration of the inhibitor. These data indicate that great care should be used when characterizing an inhibitor of mediator release by simply giving a figure for the per cent inhibition of release observed in its presence.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001491:Basophils / Q000276:immunology*; D001572:Benzofurans / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors; D003847:Deoxyglucose / Q000494:pharmacology; D017259:Dimaprit; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D013806:Theophylline / Q000494:pharmacology; D013890:Thiourea / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D001572:Benzofurans; D006634:Histamine H1 Antagonists; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; C010258:enprofen; D003847:Deoxyglucose; D013806:Theophylline; D000242:Cyclic AMP; D013890:Thiourea; D017259:Dimaprit