Salvage of the modified nucleoside ribothymidine in cultured hamster embryo cells.
RNA turnover in hamster embryo cells results in the release of ribothymidine, a methylated nucleoside formed only on intact RNA. [methyl-3H]Methionine ribothymidine polyphosphates and deoxyribothymidine polyphosphates are found in approximately equivalent amounts in the acid-soluble pool of hamster embryo cells. This observation is consistent with prior unexplained observations, in intact animals, that the methyl group of S-adenosyl methionine can be used for deoxythymidine synthesis, This is the only modified ribonucleoside known to be salvaged through pathways in normal cells. Several possible pathways for salvage were investigated.
D000818:Animals; D006224:Cricetinae; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D011247:Pregnancy; D012313:RNA / Q000235:genetics*; D014316:Tritium; D014529:Uridine / Q000031:analogs & derivatives* / Q000378:metabolism
D014316:Tritium; C009182:ribothymidine; D012313:RNA; D008715:Methionine; D014529:Uridine