Correlation of differentiation state and silver staining of nucleolar organizers in the promyelocytic leukemia cell line HL-60.
We have applied nucleolar organizer region (NOR) silver staining to the promyelocytic leukemia cell line HL-60, before and after dimethylsulfoxide (DMSO) mediated differentiation. The results demonstrated a gradual suppression of rDNA transcription during terminal maturation of these bone-marrow-derived cells and support our hypothesis that there are characteristic NOR staining profiles for different bone marrow cell types.
D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D004121:Dimethyl Sulfoxide