Lineage infidelity following exposure of T lymphoblasts (MOLT-3 cells) to 5-azacytidine.
The appearance on single leukemic blast cells of markers of at least two different lineages has been termed lineage infidelity. MOLT-3 cells, a continuous line of human T lymphoblasts, express T cell markers as defined immunologically with monoclonal antibodies. Following a single exposure to 5-azacytidine, other markers, usually associated with non-T cell lineages, appeared transiently. A stable clone with lineage infidelity was obtained by selection from colonies expressing novel markers. Marker expression followed reproducible kinetics during growth. 5-Azacytidine-treated MOLT-3 subclones may be useful models in the study of lineage infidelity and gene expression.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D002999:Clone Cells / Q000145:classification; D003114:Colony-Forming Units Assay; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D010641:Phenotype; D013601:T-Lymphocytes / Q000187:drug effects* / Q000528:radiation effects
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001374:Azacitidine