Glucocorticoid receptor in chick embryonic epidermis. Inhibition by progesterone of both the binding of glucocorticoid to the receptor and glucocorticoid-induced keratinization.
Our previous study showed that hydrocortisone (10(-8) M) induced epidermal alpha-type keratinization in 13-day-old chick embryonic tarsometatarsal skin cultured in a chemically defined medium. In this study, we show that typical glucocorticoid receptor is present in epidermal cells of chick embryonic cultured skin and that alpha-type keratinization of epidermal cells is completely prevented by the coexistence of a 100-fold excess concentration of progesterone with glucocorticoid. The physiologic importance of the receptor is suggested by some correlation between the ability of progesterone to block both the differentiation-induction and the binding of active glucocorticoid to the receptor.
D000217:Acyltransferases / Q000096:biosynthesis; D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D003900:Desoxycorticosterone / Q000494:pharmacology; D004790:Enzyme Induction; D004817:Epidermis / Q000032:analysis* / Q000378:metabolism; D005938:Glucocorticoids / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D007525:Isoelectric Focusing; D007633:Keratins / Q000096:biosynthesis*; D007700:Kinetics; D009924:Organ Culture Techniques; D011374:Progesterone / Q000494:pharmacology*; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D011987:Receptors, Steroid / Q000032:analysis*; D011503:Transglutaminases
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003900:Desoxycorticosterone; D011374:Progesterone; D007633:Keratins; D000217:Acyltransferases; D011503:Transglutaminases