A modified ELISA technique for anti-hapten antibodies.
Polystyrene tubes pretreated with glutaraldehyde in phosphate buffer were used in a solid-phase enzyme-linked immunoassary (ELISA). The hapten o-dinitrocarboxyphenol (o-DNCP) was used as coat. The binding of homologous rabbit antibodies was measured with phosphatase-labelled staphylococcal protein A (SPA-PH). This method is both very sensitive (serum dilutions greater than 2 x 10(-6) were still positive) and highly reproducible. Anti-DNP rat IgE was detected with o-DNCP hapten as a coat using avidin/biotin as a marker.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001666:Binding Sites, Antibody / Q000187:drug effects; D003959:Diamines / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005976:Glutaral / Q000494:pharmacology; D006241:Haptens / Q000276:immunology*; D011137:Polystyrenes; D011817:Rabbits; D051381:Rats
D000906:Antibodies; D003959:Diamines; D004136:Dinitrobenzenes; D000939:Epitopes; D006241:Haptens; D011137:Polystyrenes; D005976:Glutaral; C007297:1,6-diaminohexane