title: Rat gastric prepepsinogen: in vitro synthesis and partial amino-terminal signal sequence.
abstract: The major translation product of rat gastric mucosa RNA in a wheat germ cell-free system was identified as prepepsinogen by electrophoretic analysis of its immunoprecipitate on sodium dodecyl sulfate (SDS)-polyacrylamide gels and amino-terminal sequence determination. The translation product containing radioactive amino acids, purified by SDS-polyacrylamide gel electrophoresis, was shown to have an amino-terminal extension peptide comprising 16 amino acid residues. A partial amino acid sequence of this extension peptide is as follows: Met-X-X-Met-Val-Val-X-Leu-Leu-X-Leu-X-Leu-Leu-X-X-pepsinogen.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000378:metabolism; D066298:In Vitro Techniques; D020116:Pepsinogen A; D010435:Pepsinogens / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D051381:Rats
chemical_list: D004792:Enzyme Precursors; D010435:Pepsinogens; D012313:RNA; C036005:prepepsinogen; D020116:Pepsinogen A