Site of tubulin synthesis in Tetrahymena pyriformis.
Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the alpha- and beta-tubulin subunits.
D000818:Animals; D001665:Binding Sites; D007106:Immune Sera / Q000032:analysis; D066298:In Vitro Techniques; D011061:Poly A / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger; D011817:Rabbits; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D014404:Tubulin / Q000096:biosynthesis*
D007106:Immune Sera; D012333:RNA, Messenger; D014404:Tubulin; D011061:Poly A; D012313:RNA