The electron density of light and dark lysosomes in the proximal convoluted tubule of the rat kidney.
The present paper deals with the electron density of the lysosomal matrices in the renal proximal-convoluted-tubule cells of Wistar rats (20-60 days of age). Its purpose was to determine which physicochemical factors influence the electron density of lysosomes, and how their electron density is affected by various methods of fixation.--3 types of lysosomes can be distinguished in the proximal-convoluted-tubule cell; namely light, intermediate and dark lysosomes, which have an electron density lower, equal to or higher than the surrounding cytoplasm. All 3 types of lysosomes were invariably present after all methods of fixation tested. Light, intermediate and dark lysosomes differ in several respects. Staining intensity with uranyl acetate, lead citrate and potassium permanganate, osmiophilia, basophilia (in semithin sections) and --probably most importantly--the physical mass density of the lysosomal matrix are all low in light lysosomes, higher in intermediate lysosomes and highest in dark lysosomes. Light, intermediate and dark lysosomes of the proximal convoluted tubule do not form discrete classes, but one continuous spectrum of lysosomes of increasing electron density.
D000818:Animals; D002951:Citrates; D019343:Citric Acid; D003584:Cytological Techniques; D005976:Glutaral; D007684:Kidney Tubules / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D009992:Osmium; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D014501:Uranium
D002951:Citrates; D009942:Organometallic Compounds; C005460:uranyl acetate; D019343:Citric Acid; D009992:Osmium; D014501:Uranium; D005976:Glutaral