Antisera to synthetic peptide recognize high molecular weight enkephalin-containing proteins.
Antisera to a synthetic peptide corresponding to the 95-117 sequence of proenkephalin were used to develop a sensitive radioimmunoassay. Gel-filtration of acid extracts of bovine adrenal medulla and purified chromaffin granules revealed that the antisera recognized high molecular weight material (Mr approximately 5,000-30,000). The material in peak I ( Mr 20 ,000-30,000) and peak II (Mr 10,000-20,000) was further purified by immunoaffinity chromatography. Sequential digestion of each of these fractions with trypsin and carboxypeptidase B generated immunoreactive Met-enkephalin. This study demonstrates that antisera against a synthetic peptide cross-react with high molecular weight enkephalin-containing precursors, validating the use of these antisera in studies of enkephalin biosynthesis.
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis; D004745:Enkephalins / Q000032:analysis / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D007106:Immune Sera / Q000276:immunology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology*; D011498:Protein Precursors / Q000276:immunology*; D011863:Radioimmunoassay
D004745:Enkephalins; D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D011498:Protein Precursors; C029992:proenkephalin