title: Mucosal mast cells of the rat intestine: a re-evaluation of fixation and staining properties, with special reference to protein blocking and solubility of the granular glycosaminoglycan.
abstract: Mucosal mast cells of the gastrointestinal tract constitute a separate cell line within the mast cell system of the rat, differing in several respects from the classical connective tissue mast cells and, unlike the latter, requiring special fixation techniques for their demonstration. We have examined some histochemical properties of mucosal mast cells of the duodenum and compared them with connective tissue mast cells of the tongue or skin. The results indicate that the structural integrity of the granules of both types of mast cell is partly dependent on ionic linkages between glycosaminoglycan and protein. The so far unidentified glycosaminoglycan of mucosal mast cells appears to be more soluble than the heparin of connective tissue mast cells. The strongly fluorescent binding of Berberine to the granules of connective tissue mast cells and, depending on their content, of heparin is absent from mucosal mast cells, confirming previous findings which suggested that they contain a glycosaminoglycan with a lower degree of sulphation. Aldehyde fixation by routine procedures reversibly blocks the cationic dye binding of mucosal mast cell granules. The dye binding groups may be unmasked by trypsination or by long staining times of the order of several days. The results suggest that the blocking of staining by aldehydes is caused by a diffusion barrier of a protein nature. Mucosal and connective tissue mast cells thus differ with respect to the spatial arrangement of glycosaminoglycan and protein in their granules. As a result of the study a modified method for the demonstration of mucosal mast cells in tissue sections is described, based on normal formaldehyde fixation and staining in Toluidine Blue for a long time. It has some advantages over previous methods and preserves the structure of mucosal and connective tissue mast cells equally well.
mesh_terms: D000143:Acids; D000423:Alcian Blue; D000818:Animals; D001599:Berberine; D003594:Cytoplasmic Granules / Q000032:analysis; D005557:Formaldehyde; D006025:Glycosaminoglycans / Q000032:analysis*; D006652:Histological Techniques; D007413:Intestinal Mucosa / Q000166:cytology*; D007501:Iron; D008407:Mast Cells / Q000166:cytology*; D051381:Rats; D013194:Staining and Labeling; D013997:Time Factors; D014048:Tolonium Chloride
chemical_list: D000143:Acids; D006025:Glycosaminoglycans; D001599:Berberine; D014048:Tolonium Chloride; D005557:Formaldehyde; D007501:Iron; D000423:Alcian Blue