Localisation of a group of antigenic sites in complement component C3, and identification of a new fragmentation pattern.
Immunisation of rabbits or goats with denaturated human C3 reproducibly gives rise to an antiserum of restricted specificity which precipitates native C3, C3b and iC3b, but not C3c or C3d isolated from 'aged' serum. The antigenic sites recognised by the antiserum appear to be localised in a small segment of the polypeptide chain located close to the N-terminus of the C3b alpha' chain. Affinity chromatography using antibodies developed by this procedure reveals an unusual pattern of degradation of C3 which occurs on conventional 'ageing' of serum at 37 degrees C.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000918:Antibody Specificity; D003176:Complement C3 / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D006041:Goats; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits
D000596:Amino Acids; D003176:Complement C3; D000939:Epitopes; D006595:Hexosamines; D010446:Peptide Fragments