Binding of [3H]ethylenediamine di-tetrodotoxin to its solubilized receptor from excitable tissues. Binding measurements by rapid gel-filtration and receptor stabilization by phosphatidylcholine.
The molecular study of bioelectrogenesis requires the purification of the membrane proteins involved in the Na-channel electrical activity. This complex biological structure contains various binding sites for different classes of neurotoxins. Labelled forms of the blocking agent, tetrodotoxin, are used to identified and quantified the solubilized membrane proteins during the purification. Such a specific probe was synthetized in our laboratory and this work reports the experimental set-up of the binding technique. A fast-gel-filtration method has been optimized with respect to column design, centrifugation time and speed and, delay between sample application and column centrifugation.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D004593:Electrophorus; D010713:Phosphatidylcholines / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D015222:Sodium Channels; D012995:Solubility; D013779:Tetrodotoxin / Q000031:analogs & derivatives* / Q000378:metabolism
D002352:Carrier Proteins; D010713:Phosphatidylcholines; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D011092:Polyethylene Glycols; D013779:Tetrodotoxin; C005092:lubrol; C041954:N,N'-ethylenediamineditetrodotoxin