Behavioural responses induced by intrathecal injection of 5-hydroxytryptamine in mice are inhibited by a substance P antagonist, D-Pro2, D-Trp7,9-substance P.
Intrathecal injection of 5-hydroxytryptamine (5-HT) in mice produced a behavioural syndrome consisting of reciprocal hindlimb scratching and biting or licking of the hindquarters. Pretreatment with either metergoline or morphine given intrathecally inhibited the 5-HT-induced behaviour. The response to 5-HT was similar to the effect of substance P (SP), and it was found that the blocker of the substance P receptor, D-Pro2, D-Trp7,9-SP, not only reduced the effect of substance P, but also that of 5-HT. The behaviour induced by substance P was also significantly reduced by intrathecal injection of either morphine or metergoline. These findings indicate a functional interaction between substance P and 5-HT in the modulation of sensory input at the spinal level.
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D006633:Histamine Antagonists; D007278:Injections, Spinal; D008297:Male; D008711:Metergoline / Q000494:pharmacology; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D013373:Substance P / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D006633:Histamine Antagonists; D008711:Metergoline; D013373:Substance P; D009020:Morphine; C032132:substance P, prolyl(2)-tryptophan(7,9)-