[Analysis of cell surface antigen of kidney cancer by autologous sera and monoclonal antibodies].
Sera from renal cancer patients were tested for reactivity with surface antigens of cultured autologous renal cancer cells, so called "autologous typing". Reactive sera from six patients were analyzed by absorption tests with autologous, allogeneic, and xenogeneic normal and malignant cells. The absorption analysis indicated the detection of three classes of surface antigens by autologous sera, i.e. class 1, individually distinct tumor antigens, class 2, shared tumor antigens, and class 3, normal cell surface antigens. Seventeen monoclonal antibodies derived from fusions with spleen cells of mice immunized with renal cancer cell lines, which showed the class 1 or class 2 antigens, identified nine cell surface antigenic systems. None of the mouse antibodies identified in this study was succeeded in recognizing antigens that are tumor specific. Three of antigenic systems, however, showed the specificity for renal tissue, and seemed to be useful for immunopathological diagnosis.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D000939:Epitopes; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes