[Specific protein markers in monolayer hepatocyte cultures from adult rats].
By indirect immunofluorescence it has been shown that syntheses of protein A, ligandin, cytochrome P-450 PhB, and serum albumin persist in hepatocytes of adult rats during the first 2-3 days in culture. A surface protein--fibronectin--was also synthesized in cultured cells to be localized on the lower side of the free cell edge. On the 4-5th day of cultivation large regions of the lammelar cytoplasm appeared in hepatocytes accompanied by cell polarization. As a result, cells acquired a "fibroblast-like" form. During this period of cultivation, cells were characterized by the loss of cytochrome P-450 PhB, by a drastic decrease in protein A, and ligandin synthesis. At the same time, gamma-glutamyl transpeptidase, the protein characteristic of the embryonic stages, was revealed histochemically. Therefore, the impairment of tissue organization accompanying the transfer of hepatocytes into the vitro conditions results in gradual changes of their morphology, in a reduction or complete loss of some specific "adult" synthesis and activation of the "embryonic" synthesis.
D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D005353:Fibronectins / Q000096:biosynthesis; D005982:Glutathione Transferase / Q000096:biosynthesis; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D000091402:Prohibitins; D014176:Protein Biosynthesis; D051381:Rats; D012709:Serum Albumin / Q000096:biosynthesis; D013499:Surface Properties; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis; D000509:alpha-Fetoproteins / Q000096:biosynthesis; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis
D005353:Fibronectins; C000717332:Phb protein, rat; D000091402:Prohibitins; D012709:Serum Albumin; D014764:Viral Proteins; D000509:alpha-Fetoproteins; D003577:Cytochrome P-450 Enzyme System; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase
Spetsificheskie belkovye markery v monosloĭnykh kul'turakh gepatotsitov vzroslykh krys.