Studies on photohemolysis with special reference to demethylchlortetracycline.
Hemolysis induced by ultraviolet radiation as well as demethylchloretetracycline (DMCT) phototoxicity have been investigated in a model using human red blood cells. Total hemolysis for UV-B and UV-C was obtained with 8.3 and 1.9 J/cm2 respectively. DMCT was shown to have pronounced hemolytic properties causing 88% hemolysis at 50 micrograms/ml and 72 J/cm2 of UV-A. No increased hemolysis rate was seen in combination with UV-B. Several factors influencing the results were studied such as incubation time, UV-A dose, drug concentration and different methods for hemoglobin detection. Photohemolysis is an accurate tool for demonstrating DMCT phototoxicity.
D002746:Chlorpromazine / Q000494:pharmacology; D003707:Demeclocycline / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D005292:Ferricyanides / Q000494:pharmacology; D006454:Hemoglobins / Q000528:radiation effects; D006461:Hemolysis / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D010787:Photosensitivity Disorders / Q000139:chemically induced* / Q000209:etiology; D014466:Ultraviolet Rays
D005292:Ferricyanides; D006454:Hemoglobins; D003707:Demeclocycline; D002746:Chlorpromazine; C028033:potassium ferricyanide