A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.
SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) were found to contain an additional protein of 52.5 kD molecular weight (MW). This protein was identified by its reactivity with the monoclonal antibody TROMA-I as being keratin no. 8, a keratin normally present only in simple epithelia. Since this keratin is present in fetal epidermis but disappears gradually when fetal skin becomes multilayered after week 13 of development (Moll et al., Differentiation 23 (1982) 170. [23]), it suggests that SV40 virus and TPA are able to induce in human keratinocytes the reexpression of fetal characters.
D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008970:Molecular Weight; D010704:Phorbols / Q000494:pharmacology*; D011247:Pregnancy; D013539:Simian virus 40 / Q000235:genetics*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D007633:Keratins; D013755:Tetradecanoylphorbol Acetate