Purification of antibody reacting with non-enzymatically glucosylated blood group M determinants.
Agglutinins with a specificity for glucose-treated M erythrocytes were found in the serum of a healthy blood donor (Mar). The affinity of these agglutinins to glucose allowed the purification by affinity chromatography on Sephadex G-25. More than 400-fold purification was achieved in a one-step procedure. Protein bound to the column and eluted with glucose solution showed a good recovery of agglutinating activity. The major components of the purified preparation were IgG and IgM.
D000328:Adult; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D000939:Epitopes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007518:Isoantibodies / Q000032:analysis / Q000302:isolation & purification*; D008951:MNSs Blood-Group System / Q000276:immunology*; D008297:Male; D011956:Receptors, Cell Surface
D000939:Epitopes; D007518:Isoantibodies; D008951:MNSs Blood-Group System; D011956:Receptors, Cell Surface; C027786:glucose receptor