Leu-enkephalin, substance P, and somatostatin immunohistochemistry combined with the retrograde transport of horseradish peroxidase in sympathetic preganglionic neurons.
Preganglionic neurons in the lower lumbar spinal cord were labeled with horseradish peroxidase applied to the cut ends of the intermesenteric trunk in golden hamsters. A black granular reaction product was obtained in HRP-labeled cells by soaking spinal cord tissue in a cobalt chloride solution prior to treatment with diaminobenzidine (DAB) and hydrogen peroxide. Spinal cord sections containing labeled preganglionic neurons were processed with the immunoperoxidase (PAP) technique. Immunoreactive processes appeared as rust-brown beads. Concentrations of beaded processes exhibiting enkephalin-like, substance P-like (SPI) and somatostatin-like (SOMI) immunoreactivity were present around HRP-labeled preganglionic neurons in the intermediolateral cell column and in the dorsal commissural nucleus. Immunoreactivity was also present in the superficial dorsal horn, the dorsolateral funiculus and around the central canal. A prominent fiber system exhibiting SPI and SOMI extended from the ventral white matter to the ventral horn.
D000320:Adrenergic Fibers / Q000378:metabolism*; D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000378:metabolism*; D006224:Cricetinae; D004743:Enkephalin, Leucine / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D008297:Male; D008647:Mesocricetus; D009046:Motor Neurons / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013373:Substance P / Q000378:metabolism*
D013373:Substance P; D013004:Somatostatin; D004743:Enkephalin, Leucine; D006735:Horseradish Peroxidase