title: Methods for the evaluation of responses to estrogen in individual cell types or regions of the uterus.
abstract: Nongenomic responses to estrogen and the genomic responses in the different uterine cell types can be dissociated selectively. The present report describes morphometric methods for the evaluation of estrogen-induced uterine edema and of genomic responses in individual cell types. The morphometric measurements of the genomic responses correlated with a classically accepted biochemical method of genomic response evaluation in the uterus (increase in RNA/DNA). Edema correlated with the classically accepted method of evaluation of uterine water imbibition, i.e., estrogen-induced increase in uterine wet weight 6 h after hormone administration.
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D004487:Edema / Q000139:chemically induced; D004717:Endometrium / Q000166:cytology / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D009215:Myometrium / Q000166:cytology / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D012313:RNA / Q000378:metabolism; D051381:Rats; D014599:Uterus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004958:Estradiol; D012313:RNA; D004247:DNA