Simultaneous visualization of Nuclear yellow and iron-dextran complex for demonstration of branched neurons by retrograde axonal transport.
Retrogradely transported iron-dextran complex and Nuclear yellow could be demonstrated simultaneously on the same sections of rat CNS. Using combined bright-field and ultraviolet illuminations, double labeled neurons were observed. They exhibited a fluorescent nucleus and cytoplasmic Prussian blue granules. Thus, the combination of these two tracers appeared to be a convenient method for the anatomical demonstration of collaterals.
D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000648:ultrastructure*; D001921:Brain / Q000033:anatomy & histology*; D002540:Cerebral Cortex / Q000033:anatomy & histology; D003342:Corpus Striatum / Q000033:anatomy & histology; D006652:Histological Techniques; D008297:Male; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013378:Substantia Nigra / Q000033:anatomy & histology; D013787:Thalamic Nuclei / Q000033:anatomy & histology