Nephelometry of acute-phase glycoproteins by binding to concanavalin A.
Nephelometry of serum acute-phase glycoproteins by binding to concanavalin A (con-A) was compared with assays for haptoglobin and alpha 1-acid glycoprotein, and for C-reactive protein. The cutoff points for positive reactions were determined on the basis of results for a random sample (n = 130) from a middle-aged population. The sensitivity of the con-A binding assay compared favorably with that of individual acute-phase glycoproteins in a follow-up cohort of 198 patients with inflammatory joint diseases. Unlike the case in many individual acute-phase glycoprotein assays, the distribution of con-A binding values in healthy subjects is remarkably symmetrical, allowing an easy distinction between abnormal and normal values.
D000209:Acute-Phase Proteins; D000293:Adolescent; D000328:Adult; D000368:Aged; D001168:Arthritis / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D003208:Concanavalin A; D006023:Glycoproteins / Q000097:blood*; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D007120:Immunochemistry; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000379:methods; D009961:Orosomucoid / Q000032:analysis; D011485:Protein Binding
D000209:Acute-Phase Proteins; D001798:Blood Proteins; D006023:Glycoproteins; D006242:Haptoglobins; D009961:Orosomucoid; D003208:Concanavalin A; D002097:C-Reactive Protein