title: Accessory cell response to rye grass pollen extract in mice, guinea pigs and man.
abstract: The ability of murine, guinea-pig and human accessory cells to function in antigen presentation has been assayed by a T-cell proliferative response to adherent cells pulsed with rye grass pollen extract. A population of phagocytic, esterase-positive, plastic-adherent splenocytes from non-immune Balb/c mice briefly incubated with solubilized rye antigen were capable of stimulating syngeneic T lymphocytes from immune mice in an antigen-specific manner. Accessory cell function was H-2 restricted and required Ia-positive cells. Treatment with a monoclonal anti-Ia antibody impaired proliferation, whereas the presence of polyclonal rabbit anti-rye antibody increased activity. Guinea-pig peritoneal and alveolar adherent cells pulsed with up to 1 mg/ml antigen increased the response of rye-immune lymph node T cells in vitro. Proliferation was dose-dependent, despite less than 0.1% of the available antigen being cell-associated, and could be inhibited by treating the accessory cells with trypsin, sodium iodoacetate and chloroquine. Adherent human peripheral blood cells treated with rye antigen supported the proliferation of non-adherent and nylon wool-enriched mononuclear cells obtained from both grass pollen-sensitive donors and from individuals lacking either detectable serum IgE antibody or a positive skin test to rye antigen.
mesh_terms: D000328:Adult; D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D002448:Cell Adhesion; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011058:Pollen / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000939:Epitopes; D006183:H-2 Antigens