Rhodamine 123 inhibits bioenergetic function in isolated rat liver mitochondria.
Rhodamine 123 accumulates in the mitochondria of living cells and exhibits selective anticarcinoma activity. The biochemical basis of toxicity was investigated by testing the effect of the dye on isolated rat liver mitochondria. Much lower concentrations of rhodamine 123 were required to inhibit ADP-stimulated respiration and ATP synthesis in well-coupled energized mitochondria than were required to inhibit uncoupled respiration and uncoupler-stimulated ATP hydrolysis. The amount of rhodamine 123 associated with the mitochondria was several-fold greater under energized as compared to non-energized conditions, which may explain why coupled functions appeared to be more sensitive than uncoupled functions to inhibition at low concentrations of rhodamine 123. It was concluded that the site of rhodamine 123 inhibition is most likely the F0F1 ATPase complex and possibly electron transfer reactions as well.
D019297:2,4-Dinitrophenol; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects*; D005456:Fluorescent Dyes; D008930:Mitochondria, Liver / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors; D010101:Oxygen Consumption / Q000187:drug effects; D006180:Proton-Translocating ATPases; D051381:Rats; D020112:Rhodamine 123; D012235:Rhodamines / Q000494:pharmacology*; D014475:Uncoupling Agents / Q000494:pharmacology; D014966:Xanthenes / Q000494:pharmacology*
D004140:Dinitrophenols; D005456:Fluorescent Dyes; D012235:Rhodamines; D014475:Uncoupling Agents; D014966:Xanthenes; D020112:Rhodamine 123; D000244:Adenosine Diphosphate; D000226:Mitochondrial ADP, ATP Translocases; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases; D019297:2,4-Dinitrophenol