title: Identification of hypnozoites and tissue schizonts of Plasmodium vivax and P. cynomolgi by the immunoperoxidase method.
abstract: An immunoperoxidase technique was used to detect hypnozoites and liver schizonts of the primate malaria species Plasmodium vivax and P. cynomolgi bastianellii in Carnoy's-fixed sections. Anti-P. cynomolgi serum and a peroxidase-conjugated anti-monkey IgG serum rendered 7-day pre-erythrocytic forms clearly visible. The technique retains the specificity of the immunofluorescence method while having the advantage of a permanent preparation. Detection of hyponozoites by this alternative method provides further evidence for their plasmodial nature.
mesh_terms: D000818:Animals; D007124:Immunoenzyme Techniques; D008099:Liver / Q000469:parasitology*; D008253:Macaca mulatta; D002679:Pan troglodytes; D010274:Parasitology / Q000379:methods*; D010961:Plasmodium / Q000166:cytology* / Q000254:growth & development; D010966:Plasmodium vivax / Q000166:cytology* / Q000254:growth & development; D013194:Staining and Labeling