In vitro translation of human placental pregnancy-specific beta 1-glycoprotein (SP1).
Synthesis of SP1-glycoprotein by the human placenta was directly demonstrated, by in vitro translation of RNA extracted from full term and from early placentas in a cell-free wheat germ system followed by specific immunoprecipitation of the radioactively labeled nascent peptides. De novo synthesized SP1-glycoprotein in both RNA preparations accounted for 1-1.3% of total protein synthesis.
D002474:Cell-Free System; D005260:Female; D005865:Gestational Age; D006801:Humans; D007163:Immunosorbent Techniques; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D014908:Triticum
D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D012313:RNA