Differential pulse voltammetry in vivo--evidence that uric acid contributes to the indole oxidation peak.
Previous studies using differential pulse voltammetry have shown that indoleamines contribute to the oxidation peak at +280-300 mV (peak 3) measured in the rat striatum in vivo using carbon fibre electrodes. In this study, using similar techniques, it is shown that 5-hydroxyindoleacetic acid and uric acid oxidize at a similar potential (+270-290 mV) in vitro. Additionally, by microinfusing uric acid or its metabolizing enzyme uricase, it is shown that uric acid oxidation contributes to about 30% of the height of peak 3 measured in the rat striatum in vivo. These results indicate that care needs to be taken in interpreting changes in the height of the in vivo peak 3 since it is not solely due to the oxidation of brain indoleamines.
D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission; D014527:Uric Acid / Q000378:metabolism*
D014527:Uric Acid; D006897:Hydroxyindoleacetic Acid