Regulation of immune responses by I-J gene products. IV. Distinct suppressor factors derived from "nonsuppressor" A strain mice.
A/J, A/WySn (H-2a, I-Jk), and B6 (H-2b, I-Jb) mice are poly(Glu50Tyr50)(GT) nonresponders. GT preimmunization does not suppress humoral immune responses to the immunogenic form of GT, GT-MBSA (GT-methylated bovine serum albumin), in these strains. B6 mice produce an idiotypic GT-specific T cell-derived suppressor factor (GT-TsF1) after GT injection, but cannot be suppressed by this or any other GT-TsF1. H-2a mice do not produce GT-TsF1, but can be suppressed by B6 GT-TsF1. Injection of cellfree B6 GT-TsF1 into H-2a mice leads to the production of two distinct I-Jk GT-TsF. We have termed these GT-TsF2 and GT-TsF3 on the basis of their kinetic activity and antigenic markers. Anti-idiotypic I-Jk GT-TsF2 is suppressive at a time intermediate between GT-TsF1 and GT-TsF3. Idiotypic I-Jk GT-TsF3 suppresses GT-MBSA PFC responses the day of PFC assay. GT-TsF3, not GT-TsF1, suppresses B6 GT-MBSA PFC responses, indicating that these idiotypic factors are distinct. Our results establish that H-2a lack or have a defective Ts1 cell for GT suppression, and H-2b mice lack a functional Ts2 cell. These findings offer strategies for circumventing suppressor T cell defects in "nonsuppressors."
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000939:Epitopes; D005802:Genes, MHC Class II; D006462:Hemolytic Plaque Technique; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008221:Lymphoid Tissue / Q000276:immunology; D008222:Lymphokines / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D051379:Mice; D008805:Mice, Inbred A / Q000276:immunology*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000276:immunology; D014176:Protein Biosynthesis; D012710:Serum Albumin, Bovine / Q000276:immunology; D013491:Suppressor Factors, Immunologic
D000939:Epitopes; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D010455:Peptides; D013491:Suppressor Factors, Immunologic; C029180:methylated bovine serum albumin; D012710:Serum Albumin, Bovine; C027606:L-glutamic acid-L-tyrosine copolymer