Kpn I family of long-dispersed repeated DNA sequences of man: evidence for entry into genomic DNA of DNA copies of poly(A)-terminated Kpn I RNAs.
We have isolated eight cDNA clones complementary to the human Kpn I repeat and determined the base sequence of three. We have also determined a portion of the base sequences of three human Kpn I family members. The three cDNA sequences are extensively homologous with the 3' ends of the three genomic Kpn I family members and with a simian Kpn I family member recently described [Thayer, R. E. & Singer, M. F. (1983) Mol. Cell. Biol. 6, 967-973]. The genomic repeats terminate in regions of sequence rich in dAMP residues close to sequences at the 3' ends of the cDNA clones; a precise 3'-terminal nucleotide cannot be distinguished. These structural features are consistent with the dispersal of at least some Kpn I family members by entry into genomic DNA of copies of Kpn I RNA transcripts. Each cDNA contains a long poly(dAMP) homopolymer at its 3' end and either one or two A-A-T-A-A-A polyadenylylation signal sequences upstream from it, suggesting that Kpn I family members may be transcribed by RNA polymerase II.
D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D011062:Polynucleotide Adenylyltransferase