Detection of the granulocyte-specific antigen 3-fucosyl-N-acetyl-lactosamine on leukemic cells after neuraminidase treatment.
The carbohydrate antigen 3-fucosyl-N-acetyl-lactosamine (FAL) is expressed on human granulocytes and is detected by a monoclonal antibody B4.3. After neuraminidase treatment, this structure can also be detected on monocytes and on the cells of nearly all acute myeloid leukemia patients (38/39). It is then also present on the cells of a number of CALLA-positive lymphatic leukemias (8/18), but not on T-ALL and B-ALL cells. On cells of patients with AUL, the antigen is then detected in many TdT+ cases, but not in TdT- cases.
D000911:Antibodies, Monoclonal / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis*; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood / Q000276:immunology*; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000276:immunology*; D016256:Lewis X Antigen / Q000032:analysis*; D009439:Neuraminidase / Q000494:pharmacology; D009844:Oligosaccharides / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D016256:Lewis X Antigen; D009844:Oligosaccharides; D009439:Neuraminidase