Antigen-specific human T-cell factors: T-cell helper and suppressor factors, immunochemical aspects.
Some immunochemical characteristics of a T-cell-derived ovalbumin (OA) antigen-specific helper (ThF120-OA) and a corresponding OA-specific suppressor effector factor (TsF120-OA) are described. These immunoregulatory molecules are retained by columns containing either the insolubilized antigen OA or the lectin ConA, or antibodies specific for the VH-region of immunoglobulins, for the beta 2-microglobulin or for Ia-framework determinants. Neither the helper factor nor the suppressor factor showed affinity for antisera specific for immunoglobulin isotypes. In search of a constant-region-like structure in factor molecules, antisera were raised in rabbits using ThF120-OA and TsF120-OA. The results show that antisera raised against ThF120-OA recognize one or more determinants of different antigenic specificities common to human ThF. This common structure is not species-specific since it is also recognized by rabbit antisera to mouse helper factor. It is absent, however, on human TsF120-OA. In the same way, human TsF120-OA was shown to interact with rabbit antisera to human (or murine) suppressor factors of different antigenic specificities, but not with anti-ThF.
D000818:Animals; D000915:Antibody Affinity; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes / Q000032:analysis*; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000378:metabolism; D008222:Lymphokines / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology
D000939:Epitopes; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D001613:beta 2-Microglobulin