Anticryptococcal type D antibodies raised in rabbits.
Antibodies to Cryptococcus neoformans Type D have been raised in rabbits. The partially purified antibody pool was fractionated by affinity-chromatography, and it was shown that antibodies to the capsular polysaccharide of Type D were present in the pool. Neither methyl alpha-D-mannopyranoside, nor D-mannose were inhibitors of the binding of Type D polysaccharide to the antibody-pool. Thus it appears that the serum contains antibody populations which are specific for terminal side-chain carbohydrate moieties of the immunizing antigen.
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003454:Cryptococcus / Q000276:immunology*; D003455:Cryptococcus neoformans / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006241:Haptens / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008024:Ligands; D008358:Mannose / Q000276:immunology; D008766:Methylmannosides / Q000276:immunology; D008958:Models, Molecular; D011134:Polysaccharides / Q000276:immunology*; D011817:Rabbits
D000908:Antibodies, Fungal; D000939:Epitopes; D006241:Haptens; D008024:Ligands; D008766:Methylmannosides; D011134:Polysaccharides; C014426:cryptococcal polysaccharide; C008466:methylmannoside; D008358:Mannose