Electrophysiological techniques in dissociated tissue culture.
The application of electrophysiological techniques to tissue culture is still evolving. We have attempted in this chapter to give a practical summary of intracellular recording techniques used in our laboratory, as well as give some examples of new experimental strategies and electrophysiological methods that should provide further information on a number of interesting neurobiological questions. The combination of an increasing knowledge of the cell biology of cultured neurons and advances in electrophysiology should continue to be a fruitful interaction.
D000818:Animals; D046508:Culture Techniques / Q000379:methods*; D004594:Electrophysiology / Q000379:methods; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D010477:Perfusion; D013194:Staining and Labeling; D013569:Synapses / Q000502:physiology; D014732:Vibration