title: [Causes of the nonspecific reactions with antigenic erythrocyte diagnostic reagents].
abstract: The causes leading to the appearance of positive reactions in diagnostic titers (1:200 and higher), registered in the sera of healthy persons in tests carried out with the use of experimental and commercial lots of Shigella sonnei and Shigella flexneri antigenic diagnostic reagents, are analyzed. The study has shown that the appearance of nonspecific reactions may be due to the influence of the initial state of the microbial culture (the biomass used as raw material for the production of sensitin), the method of the isolation of the antigen and its sensitizing dose, the kind of the erythrocyte carrier, the method of erythrocyte fixation and the temperature conditions of the passive hemagglutination test. The ways for the elimination of positive seroconversions are outlined.
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006041:Goats / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D007158:Immunologic Techniques; D012756:Sheep / Q000276:immunology; D012763:Shigella flexneri / Q000276:immunology; D012764:Shigella sonnei / Q000276:immunology; D013045:Species Specificity
chemical_list: D000941:Antigens; D000942:Antigens, Bacterial; D000939:Epitopes; C012031:sensitin
vernacular_title: Izuchenie prichin nespetsificheskikh reaktsiĭ s antigennymi éritrotsitarnymi diagnostikumami.