Positively selected Lyt-2+ and Lyt-2- mouse T lymphocytes are comparable, after Con A stimulation, in release of IL 2 and of lymphokines acting on B cells, macrophages, and mast cells, but differ in interferon production.
Purified mouse T lymphocytes were separated into Lyt-2+ and Lyt-2- populations by the procedure of panning, in which a monoclonal rat anti-Lyt-2 antibody and dishes coated with affinity-purified mouse anti-rat Ig antibodies were used. The populations obtained were 95 to 99% pure as determined by immunofluorescence. Graded doses of these T cells were cultured with optimal mitogenic doses of concanavalin A and the 0 to 24 and 24 to 48-hr culture supernatants were collected. The dose-curve assays of the supernatants of Lyt-2+ and Lyt-2- cells showed comparable activity in interleukin 2 (IL 2) and T cell-replacing factor (TRF), assayed on antigen-stimulated culture of T-depleted spleen cells. Limiting dilution assays of IL 2-secreting precursor cells stimulated by Con A showed a high frequency of precursors in both populations, slightly higher among Lyt-2- cells. The supernatants also contained comparable levels of IPA (inducer of plasminogen activator production by the macrophages), MAF (macrophage-activating factor, assayed by induction of their cytolytic function), and MCGF (mast cells growth factor, assayed on a mast cell line). IPA and MAF were not produced with the same kinetics and in the same T cell concentration conditions as IL 2 and TRF. In contrast, interferon was principally produced by the Lyt-2+ cells.
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis*; D002469:Cell Separation; D003208:Concanavalin A / Q000096:biosynthesis / Q000494:pharmacology* / Q000502:physiology; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000502:physiology; D015848:Interleukin-5; D008222:Lymphokines / Q000502:physiology; D016215:Macrophage-Activating Factors; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D010641:Phenotype; D010960:Plasminogen Activators / Q000096:biosynthesis / Q000502:physiology; D051381:Rats; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000950:Antigens, Ly; D007376:Interleukin-2; D015848:Interleukin-5; D008222:Lymphokines; D016215:Macrophage-Activating Factors; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A; D007372:Interferons; D010960:Plasminogen Activators