Distribution of the 36 000 dalton tyrosine protein kinase substrate in drug and epidermal growth factor-treated epithelial cells.
In immunofluorescent staining the 36 000 D substrate protein (p36) of epidermal growth factor (EGF)-induced tyrosine phosphorylation is distributed relatively homogeneously in non-tumorigenic MMC-E epithelial cells. A relative lack of p36 staining is found in the nuclease and along ventral microfilament bundles of the cells. Increased concentrations of p36 are detected at the cell surface and especially at cell-cell contact sites. The bulk of p36 antigen is not redistributed by drug treatments that selectively redistribute cytokeratin, or disrupt microtubules or actin microfilaments. The gross distribution of p36 is neither affected by EGF stimulation nor by the associated rapid morphological and cytoskeletal changes. It thus appears unlikely that p36 would be a structural component of the cytoskeletal system of cultured epithelial cells.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology; D003599:Cytoskeleton / Q000032:analysis*; D003703:Demecolcine / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D007365:Intercellular Junctions / Q000032:analysis; D007633:Keratins / Q000032:analysis; D008870:Microtubules / Q000032:analysis*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis*; D011494:Protein Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases; D011506:Proteins / Q000032:analysis*
D003572:Cytochalasins; D010750:Phosphoproteins; D011506:Proteins; D015638:Cytochalasin D; D003571:Cytochalasin B; D004815:Epidermal Growth Factor; D007633:Keratins; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D003078:Colchicine; D003703:Demecolcine