Changes of the activity of acid phosphatase and beta-glucuronidase in isolated platelets of whole-body irradiated rats.
In the work presented here we paid our attention to studying changes of the activity of two lysosomal enzymes-acid phosphatase and beta-glucuronidase in platelets after whole-body irradiation and application of certain clinically applied antifibrinolytics (Antilysin Spofa and epsilon-aminocaproic acid) on the activity of these enzymes in isolated platelets. From the results it is obvious that the activity of acid phosphatase and beta-glucuronidase increases in platelets of irradiated rats with a maximum on the 11th day after irradiation. In the same time interval after irradiation the most remarkable thrombocytopenia was observed. The application of Antilysin or EACA to intact, non-irradiated rats affects remarkably neither the activity of the followed enzymes nor the count of platelets. A statistically significant thrombocytopenia, in a comparison with controls was found in whole-body irradiated rats who received EACA or Antilysin. The postirradiation increase of the level of acid phosphatase in platelets exerts a similar character in rats who received EACA or Antilysin as in those who were only irradiated with a difference that the increase of the level of this enzyme was essentially lower in all the time intervals of interest. The beta-glucuronidase level in platelets of rats, who received EACA, was essentially unchanged as compared to controls. The application of Antilysin altered remarkably the level of beta-glucuronidase in platelets of whole-body irradiated rats in a comparison with a level of this enzyme in platelets of rats who received no Antilysin. Most remarkable changes were observed in latter time intervals (11th and 15th day) when the beta-glucuronidase level in platelets of rats after the application of Antilysin was remarkably higher than that in solely irradiated rats.
D000135:Acid Phosphatase / Q000097:blood*; D015119:Aminocaproic Acid / Q000494:pharmacology; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001792:Blood Platelets / Q000201:enzymology* / Q000528:radiation effects; D005966:Glucuronidase / Q000097:blood*; D008297:Male; D051381:Rats; D013921:Thrombocytopenia / Q000209:etiology; D013997:Time Factors; D014916:Whole-Body Irradiation
D007611:Aprotinin; D000135:Acid Phosphatase; D005966:Glucuronidase; D015119:Aminocaproic Acid