Human leukemic K562 cells: differential effects of 5-azacytidine on DNA methylation of epsilon-, gamma-globin and 7SL RNA genes.
5 Azacytidine ribonucleoside (5 Aza CR), greatly enhances erythroid differentiation of the K562(h) cell line, with a sharp increase of embryonic and fetal globin gene expression. This phenomenon is correlated with the undermethylation of gamma-globin but not of epsilon-globin, as the epsilon-globin gene is already extensively undermethylated before 5AzaCR induction. By contrast no variations in both DNA methylation and expression are observed in 7SL RNA genes.
D001374:Azacitidine / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D004588:Electrophoresis, Cellulose Acetate; D005914:Globins / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008745:Methylation; D012313:RNA / Q000235:genetics*
D012313:RNA; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; C024406:endodeoxyribonuclease HpaI; D015252:Deoxyribonucleases, Type II Site-Specific; D001374:Azacitidine