title: Nucleolar silver-stained granules in maturing erythroid and granulocytic cells.
abstract: Human and rabbit erythroid and granulocytic precursors in bone marrow have been investigated to provide information concerning the number of nucleolar silver-stained granules (SSGs), which represent active interphasic nucleolar organizer regions (NORs). The differentiation and maturation of precursor cells of both investigated cell lines are characterized by a gradual decrease in number of nucleolar SSGs. In advanced maturation stages of erythroblasts or granulocytes, which are known to lose the capacity to divide, the number of nucleolar SSGs is smaller than the reported average or maximal values of NORs determined for human or rabbit cells. Since committed stem cells from both cell lines contain several times the number of nucleolar SSGs than the last dividing maturation and differentiation stages, the number of active parts of interphasic NORs in committed stem cells seems to be increased and might represent a stock for the later stages. In addition, the number of nucleolar SSGs appear to be a very convenient marker of nucleolar biosynthetic activity in individual differentiating and maturing blood cells. The differences between erythroid and granulocytic stem cells with respect to the number of nucleolar SSGs disappear during the course of further differentiation and maturation.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004900:Erythroblasts / Q000648:ultrastructure*; D006098:Granulocytes / Q000648:ultrastructure*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure*; D006801:Humans; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D011817:Rabbits; D012834:Silver; D013194:Staining and Labeling
chemical_list: D012834:Silver