title: [Changes in the polymorphism of the blood serum alkaline phosphatase in chickens undergoing subacute lindane treatment].
abstract: Investigated were a total of 123 birds of the White Leghorn breed and 50 birds of the White Plymouth Rock breed. Preliminary studies were carried out on the LD50 amount of lindane (151 mg/kg body mass) with 6 groups of chickens. The birds were divided into 4 subgroups for each breed. The first subgroup was a control one and was not given lindane, and the remaining subgroups were offered the following amounts of lindane in the course of 5 successive days: II - 1/20 LD50 (7.6 mg/kg daily); III - 1/10 LD50 (15.1 mg/kg); and IV - 1/5 LD50 (30.2 mg/kg). Each single one of the test birds was treated individually by means of a rubber tube and a syringe with a pure substance of lindane dissolved in oil. The polymorphism of alkaline phosphatase in the blood plasms of chickens was determined through horizontal electrophoresis in starch gel after the method of Gahne [14]. It was found that at subacute treatment of the birds a drop off was observed of the S1 fraction, and, as a result, the frequence of the homozygotic alkaline-phosphatase phenotypes FF and SS rose, while that of FS1 and SS1 dropped. Subacute treatment with part of the birds led to the appearance of an additional fraction F1, which accompanied the SS, SS1, and SS2 phenotypes only. The changes that took place as a result of the subacute treatment were only phenotypic ones as the F1 and S1 fractions had no hereditary character.
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D002645:Chickens / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D004592:Electrophoresis, Starch Gel; D005787:Gene Frequency / Q000187:drug effects; D005838:Genotype; D001556:Hexachlorocyclohexane / Q000008:administration & dosage*; D007527:Isoenzymes / Q000097:blood*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000187:drug effects*
chemical_list: D007527:Isoenzymes; D001556:Hexachlorocyclohexane; D000469:Alkaline Phosphatase
vernacular_title: Promeni v polimorfizma na alkalnata fosfataza v krŭvna plazma na kokoshki pri subakutno tretirane s lindan.