Cytochemical mechanisms of chromatin fluorescence and staining reactions by thionin solutions.
After staining with thionin at low concentration (less than 10(-5) M), the masses of condensed chromatin in chicken erythrocyte nuclei show a red fluorescence under green excitation, which is abolished when they become stained by using concentrations higher than 10(-4) M. The possibility that intercalative binding of a hydrophobic component from the dye solution into DNA accounts for this fluorescence reaction in chromatin is briefly discussed.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004912:Erythrocytes / Q000648:ultrastructure; D005453:Fluorescence; D009994:Osmolar Concentration; D010618:Phenanthrolines / Q000494:pharmacology; D010640:Phenothiazines; D013194:Staining and Labeling
D002843:Chromatin; D010618:Phenanthrolines; D010640:Phenothiazines; C001635:ferroin; D002110:Caffeine; C009469:thionine