Cell separation using positive immunoselective techniques.
Positive immunoselection is the direct selection and recovery of cells which express a given specificity from among a heterogeneous group of contaminating cells. A variety of methods are available to effect such separations. The principles of affinity chromatography, using solid-phase matrices or cellular immunoadsorbents, are extensively used. Liquid-phase positive immunoselection can also be performed using either a fluorescence-activated cell sorter or by using 'cellular engineering' to protect a cell from an otherwise noxious environment. The enzyme catalase coupled to specific antibody has been used for this purpose and renders cells resistant to hydrogen peroxide. The various positive immunoselection techniques available are reviewed and evaluated in the following report.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002846:Chromatography, Affinity / Q000379:methods; D000939:Epitopes; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007163:Immunosorbent Techniques; D051379:Mice; D011817:Rabbits; D013997:Time Factors
D000939:Epitopes