[Modification of the lipid component of bacterial membranes by the introduction of lipotropic compounds into lyophilized cells].
In order to elucidate potentialities of modifying the lipid component of bacterial membranes, lyophilized cells of M. lysodeikticus, E. coli and other bacteria were treated by hydrophobic compounds dissolved in the organic solvent, with the latter subsequently removed by evaporation or freezedrying prior to cell rehydration. The data obtained by means of spin-probes, fluorescent spectroscopy, electron microscopy, photoreactive label and other methods suggest that following bacteria rehydration at least part of the substance occurs in the membrane structures. The amount of the substance involved in bacterial cells depends on the type of compound and on whether bacterial cells belong to gram-positive or gram-negative microorganisms: the substance content of the latter is greater than of the former.
D004926:Escherichia coli / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D005612:Freeze Drying; D008082:Lipotropic Agents; D008563:Membrane Lipids / Q000378:metabolism* / Q000528:radiation effects; D008837:Micrococcus / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D012996:Solutions; D013113:Spin Labels; D014466:Ultraviolet Rays
D008082:Lipotropic Agents; D008563:Membrane Lipids; D012996:Solutions; D013113:Spin Labels
Modifikatsiia lipidnogo komponenta bakterial'nykh membran putem vvedeniia lipotropnykh soedineniĭ v liofilizirovannye kletki.