Passive protection of NMRI mice against Serratia marcescens: comparative efficacy of commercial human IgG immunoglobulin preparations and rabbit anti-O, -H, -K, -life cell and -protease immune sera.
Three commercial, intravenously applicable human IgG preparations passively protected NMRI mice against intraperitoneal challenge with 11 of 19 selected, serologically defined O-antigen reference strains and clinical, nosocomially significant isolates of Serratia marcescens. Conventional anti-O and anti-H, but not anti-K and anti-protease, rabbit immune sera revealed passive protective capacity against homologous challenge strains; however, anti-O rabbit immune sera failed to invariably protect against heterologous test strains that carried identical serogroup O-antigens. The passive protective efficacy of anti-live cell immune sera, derived from rabbits following recovery from experimental septicemia, was abolished through dual absorption with homologous O-cells. It was concluded that antibodies directed against O-antigens, i.e., lipopolysaccharide moieties, of the challenge strains of S. marcescens afforded passive protection.
D000372:Agglutination Tests; D000818:Animals; D000941:Antigens / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface; D004756:Enterobacteriaceae Infections / Q000517:prevention & control*; D005260:Female; D006801:Humans; D007106:Immune Sera; D007116:Immunization, Passive / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019081:O Antigens; D010447:Peptide Hydrolases / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012706:Serratia marcescens / Q000276:immunology*
D000941:Antigens; D000942:Antigens, Bacterial; D000954:Antigens, Surface; C022863:H antigen; D007106:Immune Sera; D007074:Immunoglobulin G; C030659:K antigens; D019081:O Antigens; D010447:Peptide Hydrolases