Differences in human alpha- and beta-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences.
Human beta-globin genes introduced into mouse erythroleukemia (MEL) cells by DNA cotransformation are correctly regulated when erythroid cell differentiation is induced by dimethylsulfoxide (DMSO). In contrast, cloned human alpha-globin genes are efficiently transcribed in MEL cells prior to induction, and no increase in the level of alpha-globin mRNA is observed when the cells differentiate. These observations suggest that the mechanisms by which alpha- and beta-globin genes are activated during erythroid cell differentiation are fundamentally different. Analysis of the transcription of hybrid human alpha/beta-globin genes in MEL cells revealed that the sequences responsible for differences in transcription of the intact alpha- and beta-globin genes are located on the 3' side of the mRNA capping site of the two genes, suggesting that cis-acting regulatory sequences are located within the structural genes.
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D004274:DNA, Recombinant / Q000378:metabolism; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D007942:Leukemia, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012313:RNA / Q000302:isolation & purification; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012313:RNA; D005914:Globins