Macrophage-activating factor for cytotoxicity produced by a human T-cell hybridoma.
Human MAF-C (macrophage-activation factor for cytotoxicity)-producing hybridoma H2-E3-5 was prepared by somatic cell fusion of PHA-activated peripheral blood lymphocytes with emetine/actinomycin D-treated cloned human acute lymphatic leukemia cells (CEM). The following activities were assayed: (1) macrophage-migration-inhibitory factor (MIF), (2) macrophage-activation factor for glucose consumption (MAF-G), (3) macrophage-activation factor for O2-formation (MAF-O), and (4) macrophage-activation factor for cytotoxicity (MAF-C). After anion-exchange chromatography, MAF-C activity could be distinguished from MIF and MAF-O activities. It is shown that MAF-C is not the same as MAF-G from the culture supernatants of CEM 11, a parent cell line of H2-E3-5. Furthermore, MAF-C from H2-E3-5 culture supernatants activated differentiated macrophages but not monocytes.
D000954:Antigens, Surface / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007111:Immunity, Cellular; D007372:Interferons / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis*; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000954:Antigens, Surface; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007372:Interferons