Synthesis and function of (2'-5')An: inhibition of (2'-5')An synthetase by heparin and the use of heparin-agarose for partial purification of (2'-5')An synthetase from rabbit reticulocyte lysates.
Heparin, but not a number of other acid mucopolysaccharides, inhibits (2'-5')An synthetase partially purified from rabbit reticulocytes. The effect of heparin was apparent at 10 micrograms/ml (21%). Maximum inhibition was observed at 200 micrograms/ml (80%). At the latter concentration, the dsRNA-dependent (2'-5')An synthetase activity was inhibited 80% whereas the dsRNA-independent enzyme activity was inhibited only 30%. The formation of the dimer, trimer, tetramer and high oligomers of (2'-5')An was inhibited to the same extent with the addition of heparin. Heparin covalently bound to agarose beads was shown to be a useful affinity resin for the purification of (2'-5')An synthetase from rabbit reticulocyte lysates.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006493:Heparin / Q000494:pharmacology*; D066298:In Vitro Techniques; D009841:Oligonucleotides / Q000096:biosynthesis*; D011118:Polynucleotide Ligases / Q000037:antagonists & inhibitors*; D012313:RNA / Q000378:metabolism; D011130:RNA Ligase (ATP) / Q000037:antagonists & inhibitors*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate
D009841:Oligonucleotides; D012967:Sodium Dodecyl Sulfate; D012313:RNA; D000255:Adenosine Triphosphate; D006493:Heparin; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)