Genetic control of experimental autoimmune encephalomyelitis at the level of cytotoxic lymphocytes in guinea pigs.
To analyze immunologic mechanisms involved in experimental autoimmune encephalomyelitis (EAE), a system for studying the cytotoxic reaction of guinea pig lymphocytes against syngeneic macrophages pulsed with myelin basic protein (BP) was developed. It was found that both EAE-susceptible strain 13 and EAE-resistant strain 2 animals developed cytotoxicity directed against whole BP. However, only strain 13 guinea pigs developed such cells in response to immunization with the encephalitogenic nonapeptide determinant of BP. Strain 2 macrophages appeared able to present the nonapeptide determinant to strain 13 lymphocytes. Anti-BP cytotoxic lymphocytes were found to be recruited by anti-BP initiator lymphocytes.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology; D013045:Species Specificity
D004676:Myelin Basic Protein