Differences in the cleavage of DNA by bleomycin under light (300-350 nm) and dark conditions.
Incubation of supercoiled plasmid DNA (both pBR 322 and pPLC-28) with increasing concentrations of bleomycin in the presence and absence of light (300-350 nm) showed extensive conversion of supercoiled to linear DNA which occurred at much lower levels of bleomycin (BLM) in the light than in the dark. Preliminary results indicate that superoxide dismutase (SOD) may offer DNA more protection against bleomycin in the dark than in the light conditions.
D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA; D003624:Darkness; D008027:Light; D013482:Superoxide Dismutase / Q000494:pharmacology
D001761:Bleomycin; D004247:DNA; D013482:Superoxide Dismutase