Influence of well-defined mineral fibers on proliferating cells.
The effects of well-defined asbestos and man-made mineral fibers, as well as glass and synthetic fluoroamphibole, on phagocytizing permanent rat tumor cells were tested. The following parameters were compared: cell proliferation as determined by cell count and 3H-thymidine incorporation, RNA synthesis by 3H-uridine uptake, protein synthesis by incorporation of 3H-labeled amino acids, protein content and plasma membrane permeability by release of lactic dehydrogenase. The dosage of most of the dusts was estimated gravimetrically, but for some dusts also numerically. Because of the wide range of different fibers lengths, diameters and specific weights, it was sometimes difficult to compare chemically and physically differing fiber fractions with the same fiber counts. In some cases, resulting weights are so different that a direct comparison of the conclusions is impossible. The results with fibers of diverse sources showed the same trends: the toxicity of fibers increases with increasing length and dose. In this test system we found an inhibition of DNA and RNA synthesis. Protein synthesis as measured by amino acid uptake per total cell culture decreased, but the protein content of the single cell increased as determined by the Lowry method. The increase of plasma membrane permeability as determined by lactic dehydrogenase was also dependent on fiber length and concentration. Generally the thinner the fiber, the greater the toxicity when gravimetrical dosage and the same length distributions are employed. Beyond that we can state that the toxicity of fibers from different sources with similar fiber dimensions is similar. One of the glass fiber fractions has a comparable geometry (length, diameter) to the UICC fraction of chrysotile and exhibits the same high toxicity.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000096:biosynthesis; D004391:Dust / Q000009:adverse effects* / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008903:Minerals / Q000633:toxicity*; D010316:Particle Size; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D004391:Dust; D008903:Minerals; D011506:Proteins; D012313:RNA; D004247:DNA; D007770:L-Lactate Dehydrogenase