Microinjection of interferon and 2',5'-oligoadenylate into mouse L cells and their effects on virus growth.
By means of a new type of microinjection apparatus, which has a micropipette located in a hole through the optical axis of the condenser lens, we injected interferon (IFN) or 2',5'-oligoadenylate (2-5A) into mouse L cells, and observed their antiviral effects on the multiplication of vesicular stomatitis virus (VSV). After injection, cells were infected with VSV, and labeled with [3H]uridine in the presence of actinomycin D. The proportion of cells infected with VSV which carried radioactive virus-RNA was determined by autoradiography. IFN introduced directly into L cells had no effect on the virus growth. This result supports the idea that IFN molecules exert their effect from outside the cell membrane without penetrating into the cytoplasm. 2-5A, on the other hand, was able to inhibit the growth of VSV effectively when injected into L cells. The antiviral effect was dependent on the dose of 2-5A injected, and moreover the effect was transient, since it disappeared completely after 24-h incubation.
D000227:Adenine Nucleotides / Q000008:administration & dosage*; D000818:Animals; D007372:Interferons / Q000008:administration & dosage*; D007739:L Cells / Q000382:microbiology*; D051379:Mice; D008845:Microinjections; D009841:Oligonucleotides / Q000008:administration & dosage*; D009843:Oligoribonucleotides / Q000008:administration & dosage*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000254:growth & development*
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D007372:Interferons