Measurement of thymosin alpha 1 by disassociation microELISA.
A disassociation microELISA was devised for the estimation of thymosin alpha 1, a chemically characterized thymic polypeptide isolated from bovine thymosin fraction 5. Antiserum to synthetic thymosin alpha 1 was raised in rabbits. Thymosin alpha 1 in liquid phase competed with a solid-phase-bound thymosin alpha 1 for this highly specific antibody. The method is specific, sensitive, reproducible and capable of detecting as little as 100 pg/ml of thymosin alpha 1.
D000818:Animals; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000494:pharmacology; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D013951:Thymus Hormones / Q000032:analysis*
D000939:Epitopes; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D013951:Thymus Hormones; D013947:Thymosin; D000077596:Thymalfasin