Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte.
The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities--interleukin 2, CSF, and interferon--can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 X 10(8) U/mg of protein by using ion exchange high-pressure liquid chromatography.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D007372:Interferons / Q000302:isolation & purification; D007376:Interleukin-2 / Q000302:isolation & purification; D008222:Lymphokines / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D008222:Lymphokines; D007372:Interferons