Immunochemical studies on Shigella dysenteriae type 9 bacterial polysaccharide.
On graded hydrolysis and Smith degradation, the O-somatic polysaccharide isolated from Shigella dysenteriae type 9 bacteria, strain NCTC 7919, yielded five oligosaccharides which were characterized. The positions of the O-acetyl and pyruvic acetal groups in the repeating unit were identified. Immunochemical studies indicated that D-galactose is the immunodominant sugar in the polysaccharide, and one of the oligomers, having the structure Gal-(1 leads to 3)-GlcNAc-(1 leads to 3)-Gal-(1 leads to 4)-Man, showed maximum inhibition of the homologous precipitation.
D000936:Antigen-Antibody Complex; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011233:Precipitin Tests; D012762:Shigella dysenteriae / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000939:Epitopes; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial