An in-vitro model for the study of inhibin production by rat ovarian cells.
A culture system for ovarian cells from immature rats injected with PMSG was developed to study the production of inhibin. The results showed that 2 X 10(6) cells per culture dish (60 X 15 mm) were necessary for sustained production of inhibin and that serum was an obligatory component in the medium. Serum from bovine fetuses was better than serum from newborn calves for inhibin production. The constituents responsible for the maintenance of inhibin production by fetal bovine serum could be adsorbed with 1% charcoal and 0.1% dextran.
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002606:Charcoal; D003470:Culture Media; D003911:Dextrans; D005260:Female; D005312:Fetal Blood; D007265:Inhibins / Q000096:biosynthesis*; D008954:Models, Biological; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D011372:Progestins / Q000096:biosynthesis; D051381:Rats
D003470:Culture Media; D003911:Dextrans; D011372:Progestins; D002606:Charcoal; D007265:Inhibins