Isolation and specificity of antibodies to uridine.
Antibodies to uridine were produced in rabbits using a conjugate of uridine (periodate oxidized) and bovine serum albumin as immunogen. The specificity of the antibodies was studied by estimating the inhibition of the binding of 3H-uridine-trialcohol to the antibodies by various non-radioactive related compounds, using a nitrocellulose filter assay. The antibodies were 1500-fold more reactive to the uridine-trialcohol than the parent nucleoside. They bound 3H-RNA at homologous base residues. The hapten specific antibodies were purified by affinity chromatography on a uridine-AH-Sepharose column.
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity / Q000379:methods; D012313:RNA / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D014529:Uridine / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D012710:Serum Albumin, Bovine; D012313:RNA; D014529:Uridine