Ultrastructural detection of RNA: complementarity of high-resolution autoradiography and of RNAase-gold method.
The recently developed RNAase-gold cytochemical method and the more classical high-resolution autoradiography following incorporation of tritiated uridine, were applied for the localization of RNA molecules in thin sections of isolated liver cells cultured under control conditions or submitted to drugs known to alter the distribution of nuclear RNA. The similar pattern of labeling obtained with both techniques under the three experimental conditions studied (control, treatments with CdCl2, or actinomycin D), together with the results obtained after RNAase digestion, are a good indication of the high specificity and sensitivity of the RNAase-gold method and provide a demonstration of the complementarity of these two methods for the study of the ultrastructural distribution of nuclear RNA.
D000818:Animals; D001345:Autoradiography; D003609:Dactinomycin / Q000494:pharmacology; D006046:Gold; D008099:Liver / Q000648:ultrastructure; D008722:Methods; D008854:Microscopy, Electron; D012313:RNA / Q000032:analysis*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism
D003609:Dactinomycin; D012313:RNA; D006046:Gold; D012260:Ribonucleases