[Carbohydrate components of the nuclear matrix in rat liver and hepatoma].
The Con A-peroxidase reaction has demonstrated glycoproteins with molecular masses of 200 and 50 kilodalton on the polyacrylamide gel-SDS electrophoregrams of the molecular matrices isolated from rat liver, hepatoma 27 and Zajdela's ascites hepatoma. Both hepatomas contained an additional band of about 38 kilodalton, whereas Zajdela's hepatoma distinct bands of 54 kilodalton and less demonstrable of over 200 and about 105 and 68 kilodalton. Electron microscopy showed Con A-ferritin staining, more prominent in hepatomas than in the liver, at the periphery of isolated nuclear matrix preparations. Ruthenium red staining characteristic of acidic polysaccharides (glycosaminoglycans), on the contrary, was more pronounced in liver nuclear matrices.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000032:analysis*; D051381:Rats; D012430:Ruthenium Red; D013194:Staining and Labeling
D006023:Glycoproteins; D006025:Glycosaminoglycans; D009363:Neoplasm Proteins; D003208:Concanavalin A; D012430:Ruthenium Red; D005293:Ferritins
Uglevodnye komponenty iadernogo matriksa pecheni i gepatom krysy.