title: Rat liver mitochondrial aspartate aminotransferase mRNA: synthesis of an higher molecular weight precursor by free polysomes.
abstract: The site of synthesis of the higher molecular weight precursor of mAAT has been determined by separation of free and membrane-bound polysomes. Free and bound polysomes were isolated from rat liver and the polyA+RNA was extracted. Protein synthesis was carried out for two hours by using a reticulocyte lysate system in the presence of 35S-methionine. The samples were subjected to immunoprecipitation with specific antiserum. The precipitates were analyzed by Na-DodSO4/polyacrylamide gel electrophoresis and fluorography. The results clearly demonstrate that the enzyme is synthesized by free polysomes.
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000096:biosynthesis*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D008970:Molecular Weight; D011061:Poly A / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004792:Enzyme Precursors; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D001219:Aspartate Aminotransferases