title: Re-expression of hepatic functions in mouse hepatoma X rat hepatoma hybrids.
abstract: Neonatal hepatic functions are selectively extinguished in hybrids between mouse hepatoma cells, that express only fetal hepatic functions, and rat hepatoma cells expressing neonatal as well as fetal functions. A search for hybrid cells reexpressing these neonatal functions was undertaken to determine; (1) whether the selective extinction of neonatal functions is reversible and at what frequency, and (2) whether the re-expression of neonatal functions would be accompanied by modifications in the expression of fetal functions. The criterion used to obtain hybrids showing re-expression was glucose-free medium (G) where growth requires the presence of the extinguished gluconeogenic enzymes. Even though the parental cells are of the same histotype it proved difficult to obtain re-expression. Survivors in G- were obtained only from hybrids containing a greater than 1s complement of rat chromosomes; they reexpress not only gluconeogenic enzymes but also basal tyrosine aminotransferase activity, and the fetal hepatic function alpha-fetoprotein continues to be expressed in most of the clones. All survivors in G- display a significant loss of chromosomes and this loss concerns essentially mouse chromosomes.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D005260:Female; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D006822:Hybrid Cells / Q000502:physiology*; D007621:Karyotyping; D008099:Liver / Q000502:physiology; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology*; D051379:Mice; D051381:Rats; D014444:Tyrosine Transaminase / Q000378:metabolism; D014960:X Chromosome / Q000502:physiology; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D003470:Culture Media; D000509:alpha-Fetoproteins; D014444:Tyrosine Transaminase; D005947:Glucose