Mouse interferons enhance the accumulation of a human HLA RNA and protein in transfected mouse and hamster cells.
A recombinant clone (pJY150R1.1) encoding the human major histocompatibility antigen (HLA-B7) was introduced into mouse cells and hamster cells by cotransformation with selectable genes. The exposure to mouse interferon of the cells transformed to HLA-B7+ resulted in a severalfold increase in the level of HLA antigen and RNA. The HLA-B7 clone used for the transfection includes a 670-base pair DNA sequence upstream from the coding segment. It remains to be established if the 670-base pair segment is necessary and/or sufficient to make the transcription of the HLA gene responsive to interferon.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells / Q000276:immunology; D008168:Lung; D008285:Major Histocompatibility Complex / Q000187:drug effects*; D051379:Mice; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D006680:HLA Antigens; D012313:RNA; D007372:Interferons