title: Stimulation of lipid-linked oligosaccharide assembly during oviduct differentiation.
abstract: Regulation of dolichyl phosphate-linked oligosaccharide assembly has been studied during the course of diethylstilbestrol-induced chick oviduct differentiation. Oviduct membranes from treated chicks form 4.6 times as much GlcNAc-P-P-Dol and GlcNAc2-P-P-Dol upon incubation with UDP-[14C]GlcNAc and MgCl2 than do membranes from untreated chicks. Assembly of oligosaccharide-lipid was studied by incubation of membranes with purified exogenous [14C]GlcNAc2-P-P-Dol and GDP-Man. Man transfer required a divalent cation (10 mM Mg2+) and detergent (0.5% Nonidet P-40 is optimal) and occurs in the presence of amphomycin (500 micrograms/ml). The apparent Km for GDP-Man is 1 microM and for [14C]GlcNAc2-P-P-Dol is 0.45 microM. The products are a series of sequentially formed dolichyl pyrophosphate-linked saccharides up to Man5GlcNAc2, the first of which is Man beta 1,4GlcNAc2. The same products are formed either in the presence or absence of amphomycin. Conversion of GlcNAc2-P-P-Dol to higher oligosaccharides is stimulated 3-fold by estrogen treatment of chicks. Similarly, the conversion of partially purified exogenously added Man beta-[14C]GlcNAc2-P-P-Dol is 4.6-fold higher after diethylstilbestrol treatment.
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002645:Chickens; D004054:Diethylstilbestrol / Q000494:pharmacology; D004288:Dolichol Phosphates / Q000378:metabolism; D005260:Female; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism; D008358:Mannose / Q000378:metabolism; D010057:Oviducts / Q000166:cytology*; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*
chemical_list: D004288:Dolichol Phosphates; D011104:Polyisoprenyl Phosphate Oligosaccharides; D011105:Polyisoprenyl Phosphate Sugars; C024962:dolichyl diphosphate oligosaccharides; C028306:dolichol monophosphate; D006155:Guanosine Diphosphate Mannose; D004054:Diethylstilbestrol; D008358:Mannose