[Nature of conformational restrictions in nuclear superhelical DNA from thymus lymphocytes].
The lymphocyte nucleoids of mouse thymus contain about 40% of rapidly labelled nuclear RNA, about 9% of total intracellular protein and all nuclear DNA. Relaxation of superhelical DNA after thymocyte nucleoids treatment with pronase or RNAase suggests that non-histone proteins and/or RNAs are involved in conformational restrictions in the superhelical domains of cell DNAs. Thymocyte nucleoids proteins are represented by two groups of nonhistone proteins with molecular weights of 50 000-60 000 and 75 000-85 000. An essential role in the appearance of conformational restrictions in thymocyte superhelical DNA belongs to disulfide bonds.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004278:DNA, Superhelical / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis*; D008297:Male; D051379:Mice; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000032:analysis; D011402:Pronase; D012313:RNA / Q000032:analysis; D012260:Ribonucleases; D013950:Thymus Gland / Q000032:analysis
D002868:Chromosomal Proteins, Non-Histone; D004278:DNA, Superhelical; D009698:Nucleoproteins; D012313:RNA; D012260:Ribonucleases; D011402:Pronase
Priroda konformatsionnykh ogranicheniĭ v iadernoĭ superspiral'noĭ DNK limfotsitov timusa.