title: A simple method of staining the synaptonemal complex with Coomassie brilliant blue for light microscopy.
abstract: A simple technique for staining synaptonemal complexes with Coomassie brilliant blue for light microscopy has been described. The testis cells were exposed to prolonged hypotonic treatment and dropped on Formvar-coated slides. Following fixation with paraformaldehyde the slides were stained with Coomassie brilliant blue for 15 min to 1 h at room temperature and rinsed in distilled water. For its simplicity and rapidity this technique may serve as an effective alternative to silver staining for light microscopic observation of synaptonemal complexes.
mesh_terms: D000818:Animals; D008297:Male; D008540:Meiosis; D008853:Microscopy; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D013737:Testis / Q000648:ultrastructure
chemical_list: D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue