Selective inhibition of collagen biosynthesis in the dental pulps of glucocorticoid-treated rabbits.
Prednisolone (2 mg/kg) was injected daily for 14 days. Collagen and protein biosynthesis were measured in isolated molar and incisor pulps by the incorporation of [14C]-proline into protein and collagen in vitro. Collagen solubility, free proline content, prolyl hydroxylase activity, collagenolytic activity and DNA and RNA contents were also assayed. Rabbits injected with saline or starved served as controls. Collagen synthesis was inhibited selectively in both prednisolone and starvation groups. No other aspect of collagen and protein metabolism was affected by either prednisolone treatment or starvation. Thus glucocorticoid administration reduces collagen formation in the pulp, resembling the anti-anabolic effect of starvation. Glucocorticoid treatment at high daily dosages, therefore, may disturb normal development and metabolism of teeth.
D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000378:metabolism; D003782:Dental Pulp / Q000187:drug effects / Q000378:metabolism*; D003864:Depression, Chemical; D008297:Male; D011239:Prednisolone / Q000494:pharmacology*; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D011817:Rabbits; D013217:Starvation / Q000378:metabolism
D012313:RNA; D003094:Collagen; D004247:DNA; D011392:Proline; D011239:Prednisolone; D011393:Procollagen-Proline Dioxygenase