Expression of the anti-retrovirus action of interferon in human cells which exhibit a reduced number of receptors for interferon.
The anti-viral and anti-cell fusion actions of human gamma interferon (IFN) were examined on human rhabdomyosarcoma cells and compared with the actions of IFN-alpha. Treatment of A204 and RD114-C1 cells with IFN-gamma resulted in significant inhibition of retrovirus production and cell fusions which were induced by Sendai virus, but IFN-gamma did not induce 2'-5' oligoadenylate (2-5A) synthetase or dsRNA-dependent protein kinase, and failed to inhibit EMC virus replication in RD114-C1 cells as previously observed on IFN-alpha treatment (Tomita, Y. et al. (1982) Virology 120, 258-263). Although IFN-gamma induced 56K protein more strongly than IFN-alpha in human transformed HEp-2, HeLa, RSa, IFr, and A204 cells, no significant induction of this protein was observed in RD114-C1 cells after IFN-alpha or IFN-gamma treatment. Specific bindings of 125I-labeled human IFN-alpha A to HeLa, A204 and RD114-C1 cell surfaces showed that the numbers of the binding sites on RD114-C1 cells were reduced to less than 22% of those on A204 cells. These results suggest that RD114-C1 cells exhibit a reduced number of receptors for IFN on the cell surface and that the receptors are functional for the expression of the anti-retrovirus and anti-cell fusion actions of IFN, but are not enough in number for expression of the anti-EMC virus action of IFN.
D000998:Antiviral Agents; D002478:Cells, Cultured; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D011494:Protein Kinases / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017471:Receptors, Interferon; D012190:Retroviridae / Q000187:drug effects*; D013937:Thymidine Kinase / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D011956:Receptors, Cell Surface; D017471:Receptors, Interferon; D007372:Interferons; D011494:Protein Kinases; D013937:Thymidine Kinase; D012194:RNA-Directed DNA Polymerase