A rapid method for obtaining specific antibodies to mouse alpha 1-fetoprotein.
Rabbit antiserum to whole mouse amniotic fluid can be made alpha 1-fetoprotein-specific by in vivo absorption into adult mice. The technique is easy to perform, with minimum training, and provides an average of 0.8-1.2 ml of specific antiserum in an absorption time of 5 min. The unfractionated absorbed antiserum, when precipitated with glutaraldehyde, can be used as an immunoabsorbent to purify alpha 1-fetoprotein from whole amniotic fluid, yielding an average of 100 microgram of alpha 1-fetoprotein/ml of immunoabsorbent. The isolated protein appears antigenically pure when analyzed by SDS-PAGE.
D000818:Animals; D000918:Antibody Specificity; D002846:Chromatography, Affinity / Q000379:methods; D051379:Mice; D011817:Rabbits; D000509:alpha-Fetoproteins / Q000276:immunology* / Q000302:isolation & purification
D000509:alpha-Fetoproteins