Inhibition of the genotoxicity of bleomycin by superoxide dismutase.
Bleomycin was found to be cytotoxic and mutagenic in the CHO/HGPRT forward mutation assay. Approximately 50% cell mortality was achieved after a 1-h exposure to 10 micrograms/ml BLM. Bleomycin was also found to induce mutation to thioguanine resistance in a dose-dependent manner. Both the cyto- and geno-toxicity resulting from BLM exposure could be reduced by the prior addition of superoxide dismutase, implicating a role for activated oxygen metabolites in the mechanism of toxicity of bleomycin.
D000818:Animals; D001761:Bleomycin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002460:Cell Line; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D010053:Ovary; D013482:Superoxide Dismutase / Q000494:pharmacology*
D009153:Mutagens; D001761:Bleomycin; D013482:Superoxide Dismutase; D007041:Hypoxanthine Phosphoribosyltransferase