Differentiation of mouse leukemic M1 cells induced by polyprenoids.
We screened several synthetic polyprenoids about their capacity of cell differentiation using mouse myeloid leukemic M1 cells, and found that 3, 7, 11, 15, 19,-pentamethyleicosa-2, 6, 10, 14, 18-pentaenol (Geranyl Farnesol) could induce the differentiation of M1 cells into macrophage-like cells. The optimal concentration of this compound for its induction capacity was approximately 2 X 10(-5) M. The induced differentiation related markers were morphological change in M1 cells, Fc receptors for IgG (Fc gamma), and non-specific esterase activity. However, phagocytic activity was poorly induced. This suggests that most of Geranyl Farnesol treated cells may be retained functionally incomplete macrophages. The presence of cycloheximide at 1 X 10(-7)M or the incubation of M1 cells at 4 degrees C completely blocked the induction of Fc gamma, suggesting the induction of Fc gamma required protein synthesis. The treatment of M1 cells with Geranyl Farnesol resulted in suppression of DNA and RNA synthesis. Further analysis of RNA metabolism suggested that the suppression of RNA synthesis was mainly due to the decrease of rRNA. On the other hand the synthesis and turnover of polyA-containing RNA (mRNA) rather increased.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D004950:Esterases / Q000378:metabolism; D005778:Gefarnate / Q000031:analogs & derivatives / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D009696:Nucleic Acids / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger; D011961:Receptors, Fc / Q000096:biosynthesis; D013729:Terpenes / Q000494:pharmacology*
D009696:Nucleic Acids; D012333:RNA, Messenger; D011961:Receptors, Fc; D013729:Terpenes; D005778:Gefarnate; D011061:Poly A; D012313:RNA; C043321:geranylfarnesol; D004950:Esterases