Effect of bleomycin on lipid peroxides, glutathione peroxidase and collagenase in cultured lung fibroblasts.
Bleomycin-Cu(II) complex tended to increase the lipid peroxide level in cultured lung fibroblasts, though neither free bleomycin nor free cupric ion increased the level. Simultaneous addition of DL-alpha-tocopherol decreased the level significantly. Bleomycin-Cu(II) complex decreased glutathione peroxidase activity remarkably, though free bleomycin reduced the activity only slightly. Collagenase activity was not decreased but rather increased by both free bleomycin and bleomycin-Cu(II) complex. Accordingly, the accumulation of collagen induced by bleomycin could be explained not by a decrease in collagenase activity, but by the occurrence of cross-linking of collagen due to the increased lipid peroxides.
D001761:Bleomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism*; D008168:Lung; D003012:Microbial Collagenase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*
D008054:Lipid Peroxides; D001761:Bleomycin; D003300:Copper; D010544:Peroxidases; D005979:Glutathione Peroxidase; D003012:Microbial Collagenase