title: Hormonal and cholinergic influences on pancreatic lysosomal and digestive enzymes in rats.
abstract: Hormonal and cholinergic influences on lysosomal and digestive enzyme activities in pancreatic tissue were studied in normal adult rats. Hormonal stimulation by the cholecystokinin analogue, caerulein, induced a marked enhancement of the activities of cathepsin D and N-acetyl-beta-D-glucosaminidase in pancreatic tissue, whereas the activities of amylase and lipase tended to decrease. Acid phosphatase activity was not affected. Further, caerulein was found to induce a significant increase of cathepsin D output in bile-pancreatic juice. This output largely parallelled that of amylase. Cholinergic stimulation by the muscarinic agonist carbachol, at a dose level giving the same output of amylase as caerulein, did not affect pancreatic activities of cathepsin D and N-acetyl-beta-D-glucosaminidase. Further, cholinergic stimulation induced an increase of amylase activity and a slight decrease of acid phosphatase activity in pancreatic tissue. Lipase activity was not affected. No apparent effect on cathepsin D output in bile-pancreatic juice was encountered after cholinergic stimulation. The activities of neither the digestive nor the lysosomal enzymes were influenced by the administration of secretin. The results suggest a possible lysosomal involvement in caerulein-induced secretion and/or inactivation of pancreatic digestive enzymes, whereas cholinergic stimulation seems to act through different mechanisms.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000681:Amylases / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002403:Cathepsins / Q000378:metabolism; D002108:Ceruletide / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D010275:Parasympathetic Nervous System / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology
chemical_list: D012633:Secretin; D002108:Ceruletide; D002217:Carbachol; D000681:Amylases; D000118:Acetylglucosaminidase; D002403:Cathepsins