Transcription start site and induction kinetics of the araC regulatory gene in Escherichia coli K-12.
The in vivo transcription start site of the araC message was determined by S1 nuclease mapping of hybrids formed between labeled DNA, and RNA extracted from cells grown under a variety of physiological conditions, including the interval of transient derepression following arabinose addition. Under all conditions tested, transcription initiated from the same nucleotide position at -148.
D051777:AraC Transcription Factor; D001089:Arabinose / Q000235:genetics; D001426:Bacterial Proteins; D001483:Base Sequence; D004269:DNA, Bacterial; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009693:Nucleic Acid Hybridization; D009876:Operon; D012329:RNA, Bacterial; D012097:Repressor Proteins / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D051777:AraC Transcription Factor; C497371:AraC protein, E coli; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors; D001089:Arabinose; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases