Comparison of the Du Pont aca and Dow methods for determination of high-density lipoprotein cholesterol.
We compared the Du Pont aca (phosphotungstate-enzymic cholesterol) and the Dow (dextran sulfate/Mg2+-enzymic cholesterol) methods for the determination of high-density lipoprotein cholesterol (HDLC) and total cholesterol in serum from 113 patients. The aca results for both total cholesterol and HDLC were significantly greater (p less than 0.0001) than the Dow results, the aca method overestimating the HDLC concentration (mean recovery 107.2% in serum samples with values assigned by the Centers for Disease Control). The precision of the aca method for HDLC was essentially the same as that of the Dow method. Bilirubin (up to 0.17 g/L), hemoglobin (up to 4 g/L), and slight lipemia (triglycerides up to 5.4 g/L) did not interfere with the aca method.
D001322:Autoanalysis / Q000379:methods*; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL; D016264:Dextran Sulfate; D003911:Dextrans; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D010772:Phosphotungstic Acid; D013223:Statistics as Topic
D008076:Cholesterol, HDL; D003911:Dextrans; D008075:Lipoproteins, HDL; D010772:Phosphotungstic Acid; D016264:Dextran Sulfate; D002784:Cholesterol