title: [Cell-free Pseudomonas vaccine. III. Immunochemical analysis of purified Pseudomonas aeruginosa protein antigens and protective properties of antisera against these antigens].
abstract: The immunochemical analysis of isolated and purified antigens A and B obtained from P. aeruginosa, strains 868 (serogroup O3 according to Lányi or immunotype 3/7 according to Fisher) and 170015 (serogroup O7 or immunotype 2), was carried out. Rabbit antisera to proteins A and B, as well as to the initial aqueous extracts and partially purified aqueous extracts, were obtained. Cross activity between the protein antigens of different strains was established by the methods of immunodiffusion and two-dimensional immunoelectrophoresis. Isolated proteins A and B contained both common and specific antigenic determinants detected by the method of two-dimensional immunoelectrophoresis. The immunization of rabbits with proteins A and B was found to stimulate the synthesis of protective, probably species-specific, antibodies.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; D007106:Immune Sera
vernacular_title: Beskletochnaia psevdomonas-vaktsina. Soobshchenie III. Immunokhimicheskiĭ analiz ochishchennykh belkovykh antigenov Pseudomonas aeruginosa i protektivnye svoĭstva antisyvorotok k étim antigenam.