Activation of distinct T cell subsets involved in IgM antibody response of mice by pertussis toxin from Bordetella pertussis strain Maeno.
T cell activation mechanisms in the spleen induced by pertussis toxin (PT) were investigated. The degree of T cell activation was judged from results of helper activity assessed by measuring the in vitro antitrinitrophenyl (TNP) IgM antibody response. When mice were injected intraperitoneally with 4 X 10(5) sheep erythrocytes (SRBC) and concomitantly given an intravenous injection with 10 micrograms of PT 4 days previously, significant generation of nonspecific helper T cells was observed unless primed T cells were stimulated in vitro with TNP-SRBC. Stimulation with TNP-SRBC resulted in marked suppression of helper activity because it induced generation of suppressor T cells specific for the carrier antigen.
D000276:Adjuvants, Immunologic; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology; D000939:Epitopes; D007108:Immune Tolerance; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011968:Receptors, Histamine / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D000939:Epitopes; D007075:Immunoglobulin M; D011968:Receptors, Histamine