Immunogenic and antigenic properties of the COOH-terminal hexapeptide of substance P.
Substance P (SP) and its COOH-terminal fragment (6-11) were coupled to bovine serum albumin by glutaraldehyde and used to elicit antibodies. Anti-SP and anti-SP6-11 antibodies bound to the same extent 125I-[Tyr8]-SP and 125I-[T-Lys6]-SP6-11. The latter was obtained after conjugation of the [Lys6]-SP6-11 with cold Bolton-Hunter reagent and then labelling by the chloramine-T method. In contrast to 125I-[Tyr8]-SP, 125I-[Tyr8]-SP6-11 was poorly bound by both anti-SP and anti-SP6-11 antibodies. Synthetic fragments of SP shorter than SP7-11 did not react with anti-SP6-11 antibodies. It is concluded that the antigenic determinant is within the COOH-terminal pentapeptide, and that Phe7, Phe8, Leu10 and Met11 contribute significantly to both anti-SP and anti-SP6-11 immunoreactivity.
D000818:Animals; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D011817:Rabbits; D011863:Radioimmunoassay; D013373:Substance P / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D013373:Substance P; C032292:substance P (6-11); D011761:Pyrrolidonecarboxylic Acid