Hypomethylation of ras oncogenes in primary human cancers.
We have examined the methylation status of two cellular oncogenes, c-Ha-ras and c-Ki-ras, in primary human carcinomas and the adjacent analogous normal tissues from which the tumors derived. The c-Ha-ras gene was hypomethylated in six of eight carcinomas, including five colonic adenocarcinomas and one small cell lung carcinoma, when compared to adjacent normal tissues. The c-Ki-ras gene was hypomethylated to a lesser extent in two colonic adenocarcinomas. This is the first demonstration of alterations in methylation of cellular oncogenes in human cancer.
D000230:Adenocarcinoma / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D008745:Methylation; D009369:Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011110:Polymorphism, Genetic
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases