Search for a multiple sclerosis-specific brain antigen.
Specific reactivity of multiple sclerosis (MS) cerebrospinal fluid (CSF) IgG against central nervous system (CNS) tissue has been sought. Brain proteins were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), reacted with CSF followed by 125I-Staph protein A, and developed for autoradiography. Pooled CSF from MS patients, CSF from individual MS patients, and pooled CSF from patients with other neurologic diseases and from normal patients, were used. Proteins were extracted from the white matter of MS and normal brains and from MS plaque and peri-plaque tissue. No reactivity specific for MS was observed.
D000941:Antigens / Q000134:cerebrospinal fluid / Q000276:immunology*; D001921:Brain / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology
D000941:Antigens; D000939:Epitopes; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins