Control of cyclic AMP accumulation in dog acini: its relation to amylase release.
In dispersed acini from dog pancreas, exogenous derivatives of cAMP stimulated the amylase release; 8-Br-cAMP was a more potent stimulant than Bt2-cAMP, and induced a potentiation of the amylase release stimulated by cerulein. Both secretin and vasoactive intestinal peptide (VIP), increased cellular cAMP, with a greater potency for secretin, and supramaximal concentration of secretin plus VIP increased cellular cAMP to the same degree as that obtained with secretin alone. 125I-VIP-binding studies showed that in dog pancreatic acini there is a 95% decrease in the number of VIP-preferring receptors with minor changes in receptor affinity as compared to guinea-pig pancreas. The absence of effect between secretin or VIP and cerulein in stimulating amylase release could likely be explained by the low number in VIP-preferring receptors on dog pancreatic acini.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002108:Ceruletide / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004285:Dogs; D004357:Drug Synergism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D010179:Pancreas / Q000201:enzymology / Q000378:metabolism*; D011863:Radioimmunoassay; D011953:Receptors, Cyclic AMP / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D007457:Iodine Radioisotopes; D011953:Receptors, Cyclic AMP; D015124:8-Bromo Cyclic Adenosine Monophosphate; D014660:Vasoactive Intestinal Peptide; D003994:Bucladesine; D002108:Ceruletide; D000242:Cyclic AMP; D000681:Amylases