Comparative properties of purified cytochrome P-448 from beta-naphthoflavone treated rats and rainbow trout.
1. Cytochrome P-448 from beta-naphthoflavone treated rainbow trout (Salmo gairdnerii) liver was purified and compared to purified P-448 from beta-naphthoflavone treated rats (Rattus rattus) and purified P-450 from phenobarbital induced rats. 2. The two P-448 forms had similar spectral properties, substrate specificity, sensitivity to inhibitors and regioselectivity in the metabolism of benzo(a)pyrene and testosterone. 3. Rat and trout P-448 differed in apparent monomeric mol. wt (Mr) by at least 2000 daltons, and did not share identical antigenic determinants. Both rat and trout P-448 were shown to be quite different from rat P-450 using all of the above criteria for distinguishing multiple forms.
D000818:Animals; D001564:Benzo(a)pyrene; D001571:Benzoflavones / Q000494:pharmacology*; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D001580:Benzopyrenes / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005419:Flavonoids / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000032:analysis; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D012487:Salmonidae / Q000378:metabolism*; D013045:Species Specificity; D013053:Spectrophotometry; D013379:Substrate Specificity; D013739:Testosterone / Q000378:metabolism; D014337:Trout / Q000378:metabolism*; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D001580:Benzopyrenes; D003580:Cytochromes; D000939:Epitopes; D005419:Flavonoids; D001564:Benzo(a)pyrene; D013739:Testosterone; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D010634:Phenobarbital