N-terminal amino acid sequences of precursor and mature forms of alpha-1-antitrypsin.
alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitrypsin was determined. A high content of polar amino acids was found. The partially characterized presequence of in vitro synthesized alpha 1-antitrypsin showed a cluster of hydrophobic amino acids. A pre-peptide of 24 amino acids is proposed. There is no evidence for the existence of a propeptide.
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008099:Liver / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D051381:Rats; D013045:Species Specificity; D014908:Triticum / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D011498:Protein Precursors; D012333:RNA, Messenger; D000515:alpha 1-Antitrypsin; D011061:Poly A; D012313:RNA