Potentiation of surfactant release in fetal lung by thyroid hormone action.
Thyroid hormone has been shown to accelerate fetal lung development, but the mechanisms by which this hormone acts are yet unknown. Since this hormone may act indirectly by potentiating the action of endogenous catecholamines, we studied this mechanism by measuring beta-adrenergic receptors in fetal lung. Fetal rabbits at 27 days of gestation were treated with triiodothyronine (T3), 100 micrograms/100 g, in the presence and absence of propranolol, 200 micrograms/100 g, or actinomycin D, 20 micrograms/100 g. Fetuses were killed by decapitation either after 4 or 24 h of T3 treatment. The beta-adrenergic antagonist l-[3H]dihydroalprenolol was used to directly estimate the number and affinity of beta-adrenergic receptor in lung membranes. T3 increased the number of beta-adrenergic receptors in fetal lung, but the affinity of binding did not change. The enhancement of binding capacity after 4 h of T3 treatment was not inhibited by actinomycin D. However, 24-h T3-mediated stimulation was partially blocked by actinomycin D. In addition, T3 stimulated the catecholamine content, adenylate cyclase activity, and adenosine 3',5'-cyclic monophosphate content of lung. T3 increased the lecithin-to-sphingomyelin ratio, phosphatidylglycerol, and disaturated phosphatidylcholine content of the pulmonary lavage fluid. These parameters were completely inhibited by propranolol after 4 h and partially inhibited by actinomycin D after 24 h. Thus thyroid hormone enhances lung maturation by increasing the number of beta-adrenergic receptors in fetal lung.
D000818:Animals; D002395:Catecholamines / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D004082:Dihydroalprenolol / Q000378:metabolism; D005333:Fetus / Q000502:physiology*; D008168:Lung / Q000032:analysis / Q000196:embryology* / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D011817:Rabbits / Q000196:embryology; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis; D014284:Triiodothyronine / Q000494:pharmacology*
D002395:Catecholamines; D011663:Pulmonary Surfactants; D011943:Receptors, Adrenergic, beta; D014284:Triiodothyronine; D003609:Dactinomycin; D004082:Dihydroalprenolol; D012313:RNA