Two-site immunochemiluminometric assay for human alpha 1-fetoprotein.
In this two-site immunochemiluminometric assay for human alpha 1-fetoprotein, acridinium ester-labeled monoclonal antibodies are used that have an average incorporation ratio of 0.3 mol of acridinium ester per mole of antibody. The solid-phase antibody consists of sheep anti-alpha 1-fetoprotein IgG covalently coupled to a diazonium derivative of reprecipitated aminoaryl cellulose. The assay involves a 1-h incubation after the simultaneous incubation of labeled and solid-phase antibodies. The subsequent quantification time is 10 s per tube. The assay has a working range of 20 to 246 kilo-int. units/L for CVs less than or equal to 10% and a sensitivity of detection of 1.3 kilo-int. units/L. Experiments with monoclonal antibodies labeled to an average incorporation ratio of 2.8 mol of acridinium ester per mole of antibody suggest that assay sensitivity increases with increased specific activity. alpha 1-Fetoprotein concentrations in the sera of pregnant women at 14-20 weeks of gestation, as measured by the present assay, agreed with the results of a conventional radioimmunoassay.
D000166:Acridines; D001665:Binding Sites; D005260:Female; D005865:Gestational Age; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G; D008163:Luminescent Measurements; D009436:Neural Tube Defects / Q000175:diagnosis; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011296:Prenatal Diagnosis; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000032:analysis*
D000166:Acridines; D007074:Immunoglobulin G; D000509:alpha-Fetoproteins