Solubilization of nonhistone chromosomal proteins by carboxymethyldextran for chromatography.
Chromosomal proteins extracted from calf thymus by 0.35 M NaCl were insoluble at the low salt concentration needed initially for ion-exchange chromatography. However, the addition of high-affinity carboxymethyl-dextran rendered the precipitated proteins soluble, permitting them to be fractionated by elution from DEAE-Sephacel. In this way, the separation of HMG-1 from HMG-2 was achieved, as well as a partial fractionation of the low mobility group proteins, under conditions that did not disturb the native conformation of the proteins.
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D003911:Dextrans / Q000378:metabolism*; D066298:In Vitro Techniques; D011487:Protein Conformation; D012995:Solubility
D002868:Chromosomal Proteins, Non-Histone; D003911:Dextrans; C014392:carboxymethyl dextran