title: Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.
abstract: Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of chlamydial inclusions in McCoy cells is equal to that of Giemsa staining, toluidine blue staining is easier and faster to perform.
mesh_terms: D001399:Azure Stains; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000382:microbiology; D002692:Chlamydia trachomatis; D002999:Clone Cells; D005123:Eye / Q000382:microbiology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007455:Iodine; D008297:Male; D013194:Staining and Labeling; D014048:Tolonium Chloride; D014521:Urethra / Q000382:microbiology
chemical_list: D001399:Azure Stains; D014048:Tolonium Chloride; D007455:Iodine