Threonine starvation of concanavalin A treated lymphocytes impairs DNA polymerase alpha activity.
When cultured in a threonine-deficient medium, concanavalin A treated guinea-pig lymphocytes do not incorporate tritiated thymidine. DNA polymerase activity is strongly affected. The addition of the missing amino acid is followed by an early increase in protein and RNA synthesis and a delayed rise in DNA polymerase alpha activity associated with the onset of DNA synthesis.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004257:DNA Polymerase II / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013912:Threonine / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003208:Concanavalin A; D013912:Threonine; D012313:RNA; D004247:DNA; D004257:DNA Polymerase II; D007930:Leucine; D005033:Ethylmaleimide; D013936:Thymidine; D014529:Uridine