The release of histamine from rat mast cells by chymotrypsin.
The release of histamine from rat peritoneal mast cells induced by alpha-chymotrypsin and that induced by antigen have characteristics in common. The kinetics of histamine release initiated by the two agents are similar. Both alpha-chymotrypsin and antigen release histamine in the absence of extracellular calcium, phosphatidyl serine enhances the release, and disodium cromoglycate inhibits both reactions. In contrast, extracellular alpha-chymotrypsin does not induce histamine release from cells isolated from fragments of human lung, human basophils, histamine-containing cells lavaged from the bronchial lumen of the rhesus monkey, fragments of human lung and fragments of guinea pig lung.
D000818:Animals; D000941:Antigens / Q000494:pharmacology; D002470:Cell Survival; D002918:Chymotrypsin / Q000494:pharmacology*; D004205:Cromolyn Sodium / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats
D000941:Antigens; D002918:Chymotrypsin; D004205:Cromolyn Sodium