title: Synthesis and turnover of prothrombin during experimental inflammation in rats.
abstract: The response of prothrombin to inflammatory reactions was investigated in rats. Inflammation was induced by the administration of either subcutaneous turpentine or intraperitoneal endotoxin, and its effects were studied 24 h and 48 h later. Albumin and alpha 1-acute-phase globulin served as the controls. There were only insignificant changes in plasma prothrombin concentration during inflammation which contrasts sharply with a decrease in circulating albumin by approximately 25% and an increase in alpha 1-acute-phase globulin by 300-400%. These changes were paralleled by similar changes in the incorporation of [3H]lysine into these proteins during the incubation of liver slices from rats that had been pretreated with the phlogistic agents. Prothrombin catabolism, studied using 131I-prothrombin, was increased by approximately 20%; albumin turnover, studied simultaneously with 125I-albumin, was not significantly affected, though the capillary transfer rate of albumin was significantly elevated 48 h after the induction of inflammation. It is concluded that rat prothrombin is not an acute-phase protein.
mesh_terms: D000818:Animals; D004731:Endotoxins; D005260:Female; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D011516:Prothrombin / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D004731:Endotoxins; D012709:Serum Albumin; D000511:alpha-Macroglobulins; D011516:Prothrombin; D008239:Lysine; D014425:Turpentine