Cloning and mapping of fourteen different DNA fragments containing Aspergillus nidulans 5S rRNA genes.
Fourteen different plasmids hybridizing to Aspergillus nidulans 5S rRNA were isolated from a gene bank obtained after cloning Sau3A partial digests of A. nidulans DNA in a yeast--Escherichia coli vector, pBB29. The restriction maps of these plasmids were determined. The size of the cloned fragments was 2.7-9.5 kb, 12 of the plasmids were found to code for single 5S rRNA genes and 2 coded for 2 genes. No similarity of the sequences surrounding the 5S rRNA genes was found by restriction mapping.
D001233:Aspergillus nidulans / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics*; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D011995:Recombination, Genetic; D012269:Ribosomal Proteins
D004271:DNA, Fungal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C030831:ribosomal protein S5; D004262:DNA Restriction Enzymes