Effect of 15-hydroxyeicosatetraenoic acid (15-HETE) on anti-immunoglobulin E- and calcium ionophore-induced histamine release from human leukocytes. Comparison with the effects of eicosatetraynoic acid and nordihydroguaiaretic acid.
15-Hydroxyeicosatetraenoic acid (15-HETE) was prepared by soybean lipoxygenase-mediated oxygenation of arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HETE) and subsequent reduction by NaBH4. 15-HETE was identified, purified and proved as biologically active by thin layer chromatography, high pressure liquid chromatography, gas chromatography/mass spectrometry and biological experiments on horse thrombocytes and rabbit peritoneal leukocytes. 15-HETE (0.1-40 microM) was added to peripheral leukocytes of 48 human donors (33 atopics, 15 nonatopics) which were challenged with rabbit anti-human-IgE or calcium ionophore A 23187. Its effect was compared with the effects of nordihydroguaiaretic acid (NDGA) and eicosatetraynoic acid (ETYA). NDGA and ETYA markedly inhibited histamine release (17 microM: 74 +/- 11 and 39 +/- 14,3%, respectively), whereas 15-HETE neither stimulated nor inhibited spontaneous anti-IgE- or calcium ionophore A 23187-induced histamine release.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000328:Adult; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D001095:Arachidonic Acids / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology* / Q000502:physiology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology*; D009637:Masoprocol; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D001095:Arachidonic Acids; D002396:Catechols; D006893:Hydroxyeicosatetraenoic Acids; D007166:Immunosuppressive Agents; C026408:anti-IgE antibodies; D015117:5,8,11,14-Eicosatetraynoic Acid; D007073:Immunoglobulin E; D000001:Calcimycin; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D009637:Masoprocol