A rapid method for preparation of urinary bladder epithelium for flow cytometric analysis.
A rapid and reliable method is described for preparation of urinary bladder epithelium for flow cytometric analysis. This method has proven useful for measurement of cell cycle kinetics in bladder epithelium from normal fetal, neonatal and adult mice as well as animals that have been exposed to urinary bladder toxicants. A modified Vindelov 's propidium iodide stain was instilled directly into the urinary bladder through the urethra, allowing the propidium iodide to lyse the epithelial cells and stain the DNA in the liberated nuclei. The stained epithelial cell nuclei were then removed from the lumen and analyzed by flow cytometry without centrifugation or filtration techniques. This method allows examination of epithelial cell nuclei without contamination from other tissue elements due to the fact that the propidium iodide did not act beyond the epithelial basement membrane under our experimental conditions.
D000818:Animals; D004847:Epithelial Cells; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D011419:Propidium; D013194:Staining and Labeling; D001743:Urinary Bladder / Q000166:cytology*
D011419:Propidium