Human monoclonal antibodies to myelin-associated glycoprotein are directed against the polypeptide and not the carbohydrate moiety.
We have used an approach which combines the technique of electrophoretic blotting of polyacrylamide gels with methods for the degradation of carbohydrates, to study the myelin-associated glycoprotein (MAG)-specific antibodies found in 5 patients with demyelinating neuropathy. Our results show that it is possible to deglycosylate proteins on nitrocellulose blots and then to examine their antigenicity and lectin-binding properties. With MAG, our findings suggest that the monoclonal IgM antibodies from all the patients studied have specificity for the polypeptide and not the carbohydrate moiety.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002241:Carbohydrates / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein; D010455:Peptides / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D000939:Epitopes; D007075:Immunoglobulin M; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D010455:Peptides