Cell-free synthesis of rat insulin-like growth factor II.
Total RNA extracted from a rat liver cell line (BRL 3A) that synthesizes rat insulin-like growth factor II (IGF-II) was translated in a reticulocyte lysate cell-free system. Incubation of the translation products with antisera to mature rat IGF-II (Mr 8700) selectively immunoprecipitated a Mr 21,600 protein. We propose that this protein represents pre-pro-IGF-II.
D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D007328:Insulin / Q000096:biosynthesis*; D008099:Liver; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D012313:RNA / Q000378:metabolism; D051381:Rats; D013002:Somatomedins / Q000096:biosynthesis*
D007328:Insulin; D013002:Somatomedins; D012313:RNA