Immunogenic and antigenic epitopes of immunoglobulins. VIII. A human monoclonal rheumatoid factor having specificity for a discontinuous epitope determined by histidine/arginine interchange as residue 435 of immunoglobulin G.
A monoclonal human rheumatoid factor (RF-AN) reactive with human Fc gamma is shown to recognise a discontinuous epitope dependent on the presence of histidine (His) at residue 435. RF-AN is not reactive with IgG1 paraproteins having C gamma 2 or C gamma 3 domain deletions or IgG3m (5) or Ig3m (21) proteins having arginine (Arg) at residue 435. RF-AN is reactive with IgG1, 2, 4 and IgG3m (15,16) proteins having histidine at 435.
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006639:Histidine; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D012217:Rheumatoid Factor / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D006639:Histidine; D012217:Rheumatoid Factor