[A method for testing the phagocytic capacity and activation of neutrophilic granulocytes. Enhancement after preincubation in the subjects own serum and intravenous immunoglobulin preparations (preliminary communication)].
Heat inactivated S. cerevisiae as phagocytic particle and subsequent staining with trypan blue-eosin solution is suitable to give evidence of phagocytic capacity and the number of human polymorphonuclear leucocytes which are engaged in phagocytosis. After preincubation of the yeasts with i.v. IgG-preparations--and on a higher rate--with volunteers' own serum increase of phagocytic capacity and of the number of granulocytes which take part in phagocytosis occurs. The test is suitable to compare this granulocyte function of patients under different clinical conditions depending on the underlying disease and seems to be of use to decide where clinical application of polyvalent immunoglobulin therapy is indicated.
D001790:Blood Physiological Phenomena; D004801:Eosine Yellowish-(YS); D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D014343:Trypan Blue
D007074:Immunoglobulin G; D007136:Immunoglobulins; D014343:Trypan Blue; D004801:Eosine Yellowish-(YS)
Uber ein Verfahren zur gleichzeitigen Beurteilung der Phagozytose-kapazität und -Aktivierung neutrophioler Granulozyten. Anstieg nach Präinkubatio mit Eigenserum and intravenösen Immunglobulin-Präparaten (Vorläufige Mitteilung).