Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter.
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of Mr36000 and a minor protein of Mr58000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2-3-fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic beta-cell.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D002352:Carrier Proteins / Q000276:immunology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007515:Islets of Langerhans / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis
D002352:Carrier Proteins; D009004:Monosaccharide Transport Proteins; D011506:Proteins; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine