title: Characterization of anti-idiotypic suppressor T cells (Tsid) induced after antigen priming.
abstract: The induction and fine specificity of idiotype-specific suppressor T cells (Tsid) were studied. Spleen cells from C57BL/6 mice, immunized 4 wk previously with NP-KLH, failed to express NPb3 idiotype-bearing PFC when challenged in vitro with NP-Ficoll or NP-Brucella abortus. After treatment of NP-primed responder cultures with anti-Thy-1.2 anti-serum + C, NPb idiotype-bearing B cells could be detected. This B cell subset was preferentially suppressed by the addition of T cells from NP-primed mice. With this reconstitution protocol, it was determined that suppression of the NPb idiotype-bearing portion of the B cell response was mediated by a specifically induced T cell population (Tsid) that directly suppressed NPb-bearing B cells. As with a previously described suppressor population induced with hapten-modified syngeneic spleen cells (Ts2), the Tsid population bound and was lysed by NPb idiotype-bearing serum antibodies. However, the Tsid could be distinguished from the Ts2 population because it lacked I-J determinants and functioned as an effector T cell, not an intermediary suppressor cell. Furthermore, fine specificity studies with monoclonal NP-specific antibodies expressing various levels of serologically detectable NPb idiotypic determinants indicated that unlike the Ts2, the Tsid population reacts with conventional, serologically detected members of the NPb family. The combined idiotype binding studies for the Tsid and Ts2 populations demonstrate that the fine specificity of suppressor T cell populations reflects their independent mechanisms of regulation.
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D000939:Epitopes; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009596:Nitrophenols / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D010641:Phenotype; D010648:Phenylacetates; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology* / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin