Stimulation of growth of Mycobacterium lepraemurium in cell-free liquid medium by DL-aspartic acid.
The growth of M. lepraemurium in cell-free liquid medium was strongly stimulated by addition of DL-aspartic acid at a final concentration of 0.01% to 0.02% to ND and ND-5 media containing dextran and liposome. No effect of DL-aspartic acid was observed when it was added to ND and ND-5 media without dextran and liposome. Optimal pH of the culture medium is critical for the stimulating effect of DL-aspartic acid, and it varies with the composition of the medium; the optimal pH was 6.0 in ND medium containing dextran and liposome (NDLA), and was 6.6 in NDLA medium supplemented with alpha-ketoglutarate, 1-cysteine HCl, hemin, and cytochrome c (NDLA-5). A possible mechanism of the effect of aspartic acid is discussed.
D001224:Aspartic Acid / Q000378:metabolism*; D003470:Culture Media; D003911:Dextrans / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000378:metabolism; D009167:Mycobacterium lepraemurium / Q000502:physiology*
D003470:Culture Media; D003911:Dextrans; D008081:Liposomes; D001224:Aspartic Acid