Distribution of anti-RNA antibody-binding sites on the surfaces of Ehrlich ascites tumor cells.
The distribution of anti-RNA antibody-binding sites on the surfaces of Ehrlich ascites tumor cells was studied by membrane immunofluorescence after binding anti-RNA antibody on the cell surfaces. Results showed that the cells formed caps after incubation with anti-RNA antibody at 4 degrees C and the elevation of their temperature to 37 degrees C. Pronase treatment of the cell surfaces completely inhibited reactivity with anti-RNA antibody. These results suggest that the RNAs on the surfaces of Ehrlich ascites tumor cells are linked to membrane protein membrane-bound cytoskeleton complexes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001665:Binding Sites; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis* / Q000503:physiopathology; D002462:Cell Membrane / Q000032:analysis; D002465:Cell Movement; D005260:Female; D005455:Fluorescent Antibody Technique; D007939:Leukemia L1210 / Q000276:immunology / Q000503:physiopathology; D051379:Mice; D008815:Mice, Inbred Strains; D011402:Pronase; D012313:RNA / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D012313:RNA; D011402:Pronase