A two-site sandwich radioimmunoassay of human fibroblast (beta-) interferon with monoclonal antibodies.
Monoclonal antibodies were produced against human fibroblast (beta-)interferon (IFN-beta). Four of them were directed to the determinant designated a, while the remaining 3 to another determinant named b. An IFN molecule was found to bear one each of a and b determinants arranged in such a manner that the occupation of a with the corresponding antibody did not interfere with the binding of b to its corresponding antibody, and vice versa. This allowed the development of a 2-site sandwich radioimmunoassay in which antibody to b was immobilized on wells of a microtiter plate and the bound antigen was detected by the radiolabeled antibody to a. The 2-site sandwich radioimmunoassay detected, with a high sensitivity, IFN-beta either induced from fibroblast or produced by Escherichia coli harboring the gene of IFN-beta.
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007371:Interferon-gamma