Cellular composition of a cerebral hemisphere primary culture.
In this overview attention is given to available markers and methods for characterizing cell elements in a culture system. Primary cultures from newborn rat cerebral hemispheres were grown for 14 days. The population of cells was dominated by astrocytic glial cells (60-70%), but cells with properties of macrophages, endothelial-like cells, mesenchymal-like cells, ependymal-like cells, and oligoblasts were also found. Neither mature neurons nor oligodendroglial cells were observed. The enrichment in astroglial-like cells makes the cultures a satisfactory astroglial-cell model, at least for some purposes.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology; D001921:Brain / Q000166:cytology*; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques; D004727:Endothelium / Q000166:cytology; D004805:Ependyma / Q000166:cytology; D005312:Fetal Blood; D005904:Glial Fibrillary Acidic Protein; D007158:Immunologic Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis; D008264:Macrophages / Q000166:cytology; D008648:Mesoderm / Q000166:cytology; D004676:Myelin Basic Protein / Q000032:analysis; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology; D011506:Proteins / Q000032:analysis; D051381:Rats
D003470:Culture Media; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D004676:Myelin Basic Protein; D011506:Proteins; D003994:Bucladesine