Monomeric pituitary growth hormone and prolactin variants in man characterized by immunoperoxidase electrophoresis.
Immunoperoxidase electrophoresis, combining SDS--ME--PAGE and the 'double bridge' immunoperoxidase staining was applied to crude human pituitary homogenates. With anti-hGH and anti-hPL sera, 4 hGH-related monomers were characterized: a Mr 22 000 peptide corresponding to hGH; a Mr 20 000 peptide corresponding to the known hGH variant and two unknown hGH variants (Mr 65 000 and Mr 75 000). With anti-ovine, rat and human PRL sera, 4 PRL-related monomers were immunostained: one comigrated with purified hPRL (Mr 25 000), and 3 were unknown (Mr 29 000; Mr 45 000; Mr 16 000).
D000368:Aged; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D013006:Growth Hormone / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D011388:Prolactin / Q000302:isolation & purification*; D051381:Rats; D013194:Staining and Labeling
D011388:Prolactin; D013006:Growth Hormone