Studies with antisera against peripheral nervous system myelin and myelin basic proteins. I. Effects of antiserum upon living cultures of nervous tissue.
We studied the effects of antiserum against rat peripheral nervous system (PNS) myelin, rat or chicken central nervous system myelin basic protein (BP), or rabbit P2 protein from PNS myelin on myelinated cultures containing only rat dorsal root ganglion neurons and Schwann cells. While anti-PNS myelin serum consistently produced segmental PNS demyelination, anti-BP serum and anti-P2 serum did not. The culture results suggest that the myelin PNS proteins P1 (identical to basic protein from central nervous system myelin) and P2 are not exposed on the extracellular surfaces of myelin-related Schwann cells in tissue culture.
D000818:Animals; D001921:Brain / Q000276:immunology; D046508:Culture Techniques; D005727:Ganglia, Spinal / Q000187:drug effects; D007106:Immune Sera / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D004676:Myelin Basic Protein / Q000276:immunology*; D009185:Myelin Proteins / Q000276:immunology*; D009186:Myelin Sheath / Q000187:drug effects; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology; D009474:Neurons / Q000187:drug effects; D010525:Peripheral Nerves / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects; D012584:Sciatic Nerve / Q000187:drug effects*; D013116:Spinal Cord / Q000187:drug effects*; D013126:Spinal Nerve Roots / Q000187:drug effects
D007106:Immune Sera; D004676:Myelin Basic Protein; D009185:Myelin Proteins