Use of monoclonal antibodies against avian retroviral protein p19 for competitive radioimmunoassay and immunodiffusion.
Monoclonal antibodies were used in competitive binding assays to investigate the arrangement of three epitopes on the protein p19 of avian myeloblastosis virus (AMV). It was reasoned that if the epitopes recognized by two monoclonal antibodies are physically close, the binding of one antibody will sterically block the binding of the second; conversely no blocking will occur if the epitopes are sufficiently distant. The results of these competitive binding assays demonstrated the presence of two distinct antigenic sites on the protein p19. Monoclonal antibodies against the protein p19 of AMV were tested also in gel double immunodiffusion. Since the p19 protein has strong tendency to aggregate, it was not surprising, that clear precipitin lines with these monoclonal antibodies were obtained.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001354:Avian Leukosis Virus / Q000276:immunology*; D009189:Avian Myeloblastosis Virus / Q000276:immunology*; D001667:Binding, Competitive; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas; D005779:Immunodiffusion; D007457:Iodine Radioisotopes; D051379:Mice; D011863:Radioimmunoassay; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007457:Iodine Radioisotopes; D014758:Viral Core Proteins; D014764:Viral Proteins