Effect of topically applied steroidal and nonsteroidal anti-inflammatory agents on skin repair and regeneration.
We studied the effects of topically applied steroidal and nonsteroidal anti-inflammatory agents on dermal and epidermal wound healing. Superficial wounds (0.3 mm deep) on the skin of domestic pigs were treated daily with either 0.1% triamcinolone acetonide (TA), 1% hydrocortisone (HC), 1% nandrolone decanoate (ND), 1% ND + 0.1% TA, 10 mg ibuprofen, 10 mg meclofenamate sodium, 3 mg indomethacin, vehicle (USP petrolatum or 70% ethanol), or control (untreated). Wounds were excised on days 2-7 after wounding and the epidermis was separated from the dermis. The dermis was assayed for collagen biosynthesis and the epidermis was evaluated for reepithelialization. A significant decrease (P less than 0.01) in relative collagen synthesis was observed in the wounded dermis in both HC- and TA-treated groups on day 3 after wounding, but there were no significant differences on days 4-7. Depressed collagen and noncollagenous protein production was also noted in vehicle-treated wounds on day 3. Topical application of ND did not affect collagen synthesis, but when combined with TA it eliminated the inhibitory effect observed as a result of TA alone. Topical ND accelerated wound reepithelialization by 12.5% compared with vehicle and by 26% compared with untreated controls. TA delayed epidermal resurfacing by 22%, but when combined with ND (ND + TA) the rate of reepithelialization was similar to vehicle-treated wounds. HC enhanced resurfacing when compared with untreated wounds but did not differ markedly from its vehicle. The nonsteroidal anti-inflammatory drugs when topically applied markedly reduced inflammation (erythema, heat, and edema) but did not influence the healing process.
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis; D006854:Hydrocortisone / Q000494:pharmacology; D007052:Ibuprofen / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism; D008469:Meclofenamic Acid / Q000494:pharmacology; D009277:Nandrolone / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000293:injuries; D013552:Swine; D014222:Triamcinolone Acetonide / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D008469:Meclofenamic Acid; D007633:Keratins; D009277:Nandrolone; D003094:Collagen; D014222:Triamcinolone Acetonide; D006854:Hydrocortisone; D007052:Ibuprofen; D007213:Indomethacin