Effect of colchicine on mammalian liver nuclear envelope and on nucleo-cytoplasmic RNA transport.
The binding of colchicine to nuclear envelopes was studied in order to elucidate the mechanism whereby this compound inhibits nucleocytoplasmic RNA transport. The results suggest that a single class of colchicine-binding site (dissociation constant=approx. 0.7 mM, concentration=approx. 330 nmol colchicine/mg protein) is localised in the nuclear periphery (pore-lamina) and that binding to these sites effects a constriction of the pore-complexes with concomitant inhibition of RNA egress and disordering of the nuclear membrane phospholipid bilayers.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism*; D003864:Depression, Chemical; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D051381:Rats
D012313:RNA; D003078:Colchicine