Antigen-specific T contrasuppressor factor in cell-mediated immunity: interactions leading to eradication of the tolerant state.
Interactions between a T cell-derived, antigen-specific, contrasuppressor factor (TcsF) and immune T cells that block the action of T suppressor factors and allow the transfer of cellular immunity into tolerant recipients are described. Immune T cells from contact-sensitized donors are capable of transferring specific immunity into normal recipients but not into animals rendered tolerant to the specific antigen. Brief exposure of the immune cells to the TcsF enables the effective transfer of immunity into such tolerant recipients. In addition, treated immune cells become resistant to subsequent exposure to T suppressor factor (capable of inhibiting transfer of immunity to normal recipients). A cyclophosphamide-sensitive, I-J+, Ly-2 T transducer cell is required in the immune donor cell population for contrasuppression to be induced by the TcsF plus specific antigen. These cells release an antigen-non-specific contrasuppressive factor capable of rendering immune targets, depleted of transducer cells, resistant to suppression (either by suppressor factor or in the tolerant recipient). The results indicate that contrasuppression in contact sensitivity is antigen specific and that the balance of suppression and contrasuppression determines tolerance vs responsiveness in this system. The symmetrical resemblance of the contrasuppressive interactions to those of suppression in contact sensitivity are discussed.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D003520:Cyclophosphamide / Q000494:pharmacology; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007108:Immune Tolerance; D007111:Immunity, Cellular / Q000187:drug effects; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D013491:Suppressor Factors, Immunologic; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
D000950:Antigens, Ly; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D003520:Cyclophosphamide