title: A rapid and sensitive assay for protein disulphide isomerase activity.
abstract: An assay procedure for the determination of protein disulphide isomerase activity is presented. The method is based on the reactivation of randomly cross-linked RNAase, the extent of RNAase reactivation being determined from the degradation of radioactively labelled RNA. The method is rapid and sensitive and allows one to test a large number of samples simultaneously.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts; D007535:Isomerases / Q000032:analysis* / Q000378:metabolism; D008722:Methods; D019704:Protein Disulfide-Isomerases; D012313:RNA / Q000302:isolation & purification; D012260:Ribonucleases / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
chemical_list: D012313:RNA; D004492:Edetic Acid; D012260:Ribonucleases; D007535:Isomerases; D019704:Protein Disulfide-Isomerases