title: Studies on the mechanism of temperature sensitivity of infectious pancreatic necrosis virus replication.
abstract: Yields of infectious pancreatic necrosis virus from fathead minnow cell cultures were maximal at 20 degrees C. The virus failed to replicate at 28 degrees C and neither virus-specific mRNA nor virus-specific polypeptides could be detected when infected cells were maintained at this temperature. Intrinsic thermolability of virus infectivity or inability to adsorb to cells at 28 degrees C could not account for the temperature-dependent block in virus morphogenesis. Analysis of infectious virus production and virus-specific polypeptide and RNA synthesis following shifts from the permissive (20 degrees C) to the non-permissive temperature (28 degrees C) at various times after infection indicated that multiple temperature-sensitive (ts) steps were involved in the inhibition of virus replication.
mesh_terms: D000818:Animals; D002347:Carps; D002478:Cells, Cultured; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D012087:Reoviridae / Q000254:growth & development*; D012474:Salmon; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012313:RNA