Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure.
Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. This affinity chromatography preceded by molecular sieve chromatography provides a simple two-step purification procedure of PAPP-A from late pregnancy plasma. One hundred percent of the applied PAPP-A was recovered, with more than 40% being electrophoretically homogeneous after the two procedures. The remaining PAPP-A could be purified by negative affinity chromatography on anti-total human serum immobilized on agarose.
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D006493:Heparin; D006801:Humans; D007122:Immunoelectrophoresis; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D011266:Pregnancy-Associated Plasma Protein-A / Q000302:isolation & purification*; D012685:Sepharose
D011257:Pregnancy Proteins; D006493:Heparin; D012685:Sepharose; D011266:Pregnancy-Associated Plasma Protein-A