title: Characterization of the gamma-interferon-mediated induction of antigen-presenting ability in P388D1 cells.
abstract: We characterized an assay system to study the lymphokine-mediated induction of antigen-presenting ability in P388D1 cells. The ability of lymphokine-induced P388D1 macrophages to present antigen plus Id was measured by their ability to induce interleukin 2 production by antigen-specific, Id-restricted T cell hybridomas in the presence of the appropriate antigen. The production of IL 2 by the T cell hybridomas is known to be dependent on the expression of Ia antigens by the antigen-presenting cells. The results obtained suggest that a factor present in the supernatant of the T cell hybridoma FS7-20.6.18 is responsible for inducing the appearance of I-Ad and I-Ed on P388D1, measured by immunofluorescence, and the ability of the cell to present antigen in association with I-Ad or I-Ed. The factor mediating the induction of antigen-presenting ability is thought to be gamma-interferon, because the hybridoma FS7-20.6.18 is known to produce this lymphokine and the factor is sensitive to pH 2 incubation. gamma-Interferon produced by recombinant DNA technology was found to induce antigen-presenting ability in this assay; however, alpha- and beta-interferon were inactive. This observation suggests a unique immunoregulatory role for gamma-interferon. Using many T cell hybridomas in the assay, we were able to distinguish three groups: a) high avidity hybridomas that respond to antigen presented by uninduced P388D1 but show an enhanced response to antigen plus induced P388D1; b) medium avidity hybridomas that do not respond to antigen presented by uninduced P388D1; and c) low avidity hybridomas that show a limited response to antigen presented by induced P388D1, but the response of which increases if the P388D1 cells are induced for longer periods of time. These different patterns of response are believed to be dependent on the Ia antigen density expressed by the gamma-interferon-induced presenting cells, and suggest that the T cell receptors for Ag/Id display marked heterogeneity in their avidities for Ag/Id.
mesh_terms: D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000502:physiology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006825:Hybridomas / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007371:Interferon-gamma / Q000502:physiology*; D007700:Kinetics; D007941:Leukemia P388 / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A; D007371:Interferon-gamma