Improved methodology for analysis and quantitation of proteins on one-dimensional silver-stained slab gels.
A sodium dodecyl sulfate-discontinuous polyacrylamide gel electrophoresis system for separation and quantitation of low-molecular-weight (75 to 10K Da) proteins from single muscle fibers is described. Slab gels (0.75 mm thick) were stained using an improved silver-stain technique which does not require photographic fixers in order to achieve low-level background staining. The modified staining procedure uses continuous flow washing to minimize the handling of gels. The procedure has high sensitivity and gave a linear response between approximately 2 and 70 ng of protein per band. In addition, a convenient method for mounting slab gels for photography, scanning, and long-term storage has been developed.
D000818:Animals; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D011817:Rabbits; D012834:Silver; D013194:Staining and Labeling
D009124:Muscle Proteins; D012834:Silver