Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src.
Transformation of cells by Rous sarcoma virus is mediated by the product of the viral src gene, pp60src. A hybridoma cell line producing an immunoglobulin G3 antibody to pp60src was isolated after lymph node cells from immune mice were fused with mouse myeloma cells (P3-NS1-1). Mice were immunized with p60src purified from Escherichia coli cells expressing the src gene product. The monoclonal antibody immunoprecipitated pp60src from Rous sarcoma virus-transformed cells and recognized an antigenic determinant located in the amino-terminal third of the pp60src protein.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000302:isolation & purification*; D001358:Avian Sarcoma Viruses / Q000276:immunology*; D000939:Epitopes; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D015688:Oncogene Protein pp60(v-src); D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D014764:Viral Proteins; D015688:Oncogene Protein pp60(v-src)