Subcellular distribution of spin-labeled bithiazoles and bleomycin in living KB cells: an ESR study.
Spin-labeled derivatives of bithiazole and bleomycin were studied with respect to their uptake and localization in KB cells in vivo. It was found that the amidification of the C-terminal carboxylic group of bleomycinic acid was essential for the penetration of the probes in the cells and that the subcellular localization depended on the number and spacing of positively charged groups.
D001761:Bleomycin / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003497:Cyclic N-Oxides; D003593:Cytoplasm / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007624:KB Cells / Q000378:metabolism* / Q000648:ultrastructure; D013113:Spin Labels; D013844:Thiazoles / Q000378:metabolism
D003497:Cyclic N-Oxides; D013113:Spin Labels; D013844:Thiazoles; D001761:Bleomycin