Protective effects of exogenous secretin on ceruletide-induced acute pancreatitis in the rat.
Unconscious rats given intravenous ceruletide (diethylamine salt of the decapeptide caerulein) in large pharmacologic doses consistently developed moderate acute pancreatitis by 3 h and florid pancreatitis by 6 h. Biochemical serum markers of acute pancreatitis tended to parallel the severity of the pancreatic damage. In 50% of the rats, mesenteric fat necrosis was present, free peritoneal fluid containing massive elevations of trypsinogen and amylase were noted in most animals. Intravenous secretion at a low dose given simultaneously with ceruletide exerted a variable protective effect on the pathological process. A high dose of secretin produced a striking macroscopic, microscopic, and biochemical protective effect on ceruletide-induced pancreatitis. High resolution light microscopy and electron microscopy showed a marked cellular disorganization in the acini of animals treated with ceruletide alone. By contrast, there was a striking apical redirection of zymogen granules in acini of the animals treated with secretin. The results of this study suggest that high dose intravenous secretin may exert a beneficial effect on acute pancreatitis.
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D002108:Ceruletide / Q000008:administration & dosage*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D005218:Fat Necrosis / Q000473:pathology; D008049:Lipase / Q000097:blood; D008297:Male; D010179:Pancreas / Q000648:ultrastructure; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000627:therapeutic use*; D014362:Trypsinogen / Q000097:blood
D012633:Secretin; D002108:Ceruletide; D014362:Trypsinogen; D008049:Lipase; D000681:Amylases