Epitopes of peptide 43-88 of guinea pig myelin basic protein: localization with monoclonal antibodies.
Two monoclonal antibodies, one raised by immunization with mouse myelin basic protein (MBP) and the second raised by immunization with peptide 68-88 of guinea pig MBP, were compared with respect to specificity. The former antibody (15.32) cross-reacted completely with rat, guinea pig, human, and bovine MBP. It also reacted with peptide 43-88 from each MBP. The latter antibody (22.17) was nonreactive with MBP, but cross-reacted with peptide 43-88 from rat, human, guinea pig, and bovine MBP. When tested with small peptides derived from peptide 43-88, antibody 22.17 reacted with an epitope in the C-terminal region. Antibody 15.32 reacted with an epitope in the N-terminal half of the peptide. The data show that 22.17 reacted with a unique epitope associated only with free peptide, whereas 15.32 recognized an epitope common to both peptide 43-88 and MBP.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D004676:Myelin Basic Protein / Q000032:analysis* / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D000939:Epitopes; D004676:Myelin Basic Protein; D010446:Peptide Fragments; C037147:myelin basic protein 43-88