Nucleic acid spectrophotometry on paper disks.
A bichromatic spectrophotometry study in the near UV spectral range was performed on paper disks impregnated with DNA and RNA in NaCl solutions of different concentrations. Absorbance curves plotted versus a NaCl molarity log-scale degree of polymerization and the solubility of the polynucleotide. The -a/b value, determined by a previously reported equation, appeared to be constant for a given nucleic acid, irrespective of the wavelength in the spectral range 205-280 nm. This phenomenon cannot be attributed to a heterogeneous macroscopic distribution of the nucleic acid throughout the disk.
D004247:DNA; D004277:DNA, Single-Stranded; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D012313:RNA; D012965:Sodium Chloride; D013056:Spectrophotometry, Ultraviolet
D004277:DNA, Single-Stranded; D046911:Macromolecular Substances; D012965:Sodium Chloride; D012313:RNA; D004247:DNA