Isolation of a Biologically active fragment of human alpha interferon.
When treated with pepsin under limited digestion conditions, human leukocyte-derived alpha interferon (HulFN-alpha) is partially inactivated. While the initial HulFN-alpha preparation is about equally active on human and on bovine cells, the residual antiviral activity of HulFN-alpha after pepsin treatment is significantly more active on bovine cells than on human cells. Furthermore, pepsin-treated HulFN-alpha, when assayed on bovine cells, yields an active production which is smaller and antigenically distinguishable from the native forms of HulFN-alpha.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D007372:Interferons / Q000032:analysis* / Q000276:immunology; D009500:Neutralization Tests; D010434:Pepsin A / Q000494:pharmacology; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology*
D007106:Immune Sera; D010446:Peptide Fragments; D007372:Interferons; D010434:Pepsin A