Gel filtration through Sephadex G25 of virus fluorescent compound systems. Comparison with ultracentrifugation results.
A stable interaction could be made evident between influenza A(H1N1) or Sendai virus and two fluorescent naphthalic acid derivatives solubilized in DMSO. The virus-compound interaction was studied spectrophotometrically and spectrofluorometrically, at the wavelengths characteristic of virus protein and of the two compounds, in samples subjected to gel filtration or ultracentrifugation. The percentages of virus-bound compound obtained by the two methods were similar in the case of the vinyl-sulfonic derivative (affecting virus structure to a lesser extent) and considerably different in that of the isocyano derivative (causing important structural damage).
D002850:Chromatography, Gel; D003911:Dextrans; D005456:Fluorescent Dyes; D009980:Influenza A virus; D010222:Parainfluenza Virus 1, Human; D013057:Spectrum Analysis; D014461:Ultracentrifugation
D003911:Dextrans; D005456:Fluorescent Dyes; C025614:sephadex