[Comparative study of the biological properties of plasmid and natural human interferons].
A comparative study of biological properties of natural and plasmid human interferons was carried out. Natural and leukocyte interferons: alpha (induced by Newcastle disease virus) and gamma (induced by staphylococcal enterotoxin A) as well as natural fibroblastic beta interferon induced by poly(I) X poly(C) were studied in comparison with plasmid interferons alpha-F and alpha-F/D obtained from recombinant bacteria. Antigenic determinants of plasmid interferons alpha-F and alpha-F/D were found to be identical with those of natural and alpha-interferon of man and to differ from those of natural human alpha- and beta-interferons. Both plasmid interferons demonstrated the kinetics of development of the state of resistance to viruses in a human diploid cell culture typical of alpha-interferon but not of gamma-interferon from human leukocytes. Plasmid and natural alpha-interferons have similar anticellular activity for human tumor HeLa cells, similarly activate natural human killer cells and are similarly stabilized in the presence of 0.01 M lantan chloride. All these data permit a conclusion that plasmid human interferons alpha-F and alpha-F/D are analogous and close to the total preparation of natural alpha-interferon from human leukocytes. On the other hand, the range of cells sensitive to the antiviral effect of alpha-F and alpha-F/D interferons is wider than for leukocyte alpha-interferon, and stability on storage and heating is higher.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002642:Chick Embryo; D004274:DNA, Recombinant; D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects; D004686:Encephalomyocarditis virus / Q000187:drug effects; D004926:Escherichia coli; D006801:Humans; D007372:Interferons / Q000276:immunology / Q000494:pharmacology*; D007700:Kinetics; D010957:Plasmids; D014616:Vaccinia virus / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D014776:Virus Cultivation
D000941:Antigens; D004274:DNA, Recombinant; D007372:Interferons
Sravnitel'noe izuchenie biologicheskikh svoĭstv plazmidnykh i prirodnykh chelovecheskikh interferonov.