Inhibition of canine lung angiotensin converting enzyme by substance P.
We have studied inhibition of purified canine angiotensin converting enzyme by substance P and its nonapeptide derivative using Hip-His-Leu as the substrate. Kinetic studies indicated that both substance P and its nonapeptide derivative inhibited the hydrolysis of Hip-His-Leu at different concentrations. The mode of inhibition was competitive with a Ki of 1.15 microM for substance P. These results indicate that substance P is a potent inhibitor of angiotensin converting enzyme in vitro.
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D004285:Dogs; D066298:In Vitro Techniques; D007700:Kinetics; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D013373:Substance P / Q000494:pharmacology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D013373:Substance P