[Detection and characterization of the plasmids of the plague microbe which determine the synthesis of pesticin I, fraction I antigen and "mouse" toxin exotoxin].
Plasmid DNA was isolated from Yersinia pestis strains containing pesticin I or fraction I antigen and "mouse" toxin determinants. Specificity of DNA preparations was studied by using them for transformation of plague agent strains carrying no plasmids. pPstI plasmid (molecular weight 7,0-7,8 MD) encoded pesticin I, fibrinolysin and plasmacoagulase synthesis. Fraction I antigen and "mouse" toxin production determinants were borne on pFraI/Tox plasmid (molecular weight about 50 MD). The observation that some Y. pestis cultures, having lost the ability to synthesize one of pFraI/Tox products, still retained this plasmid in their cells, is regarded as an evidence for a complicated regulation of pFraI/Tox function.
D000942:Antigens, Bacterial / Q000235:genetics*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D000939:Epitopes / Q000235:genetics; D005098:Exotoxins / Q000096:biosynthesis* / Q000235:genetics; D008854:Microscopy, Electron; D010957:Plasmids; D015010:Yersinia pestis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D000942:Antigens, Bacterial; D001430:Bacteriocins; D004269:DNA, Bacterial; D000939:Epitopes; D005098:Exotoxins; C011330:Pesticin
Vyiavlenie i kharakteristika plazmid chumnogo mikroba, determiniruiushchikh sintez pestitsina I, antigena fraktsiia I i ékzotoksina "myshinogo" toksina.