Silver staining of high molecular weight proteins on large-pore polyacrylamide gels.
A large-pore gel for electrophoresis in the presence of sodium dodecyl sulfate, composed of 2.55% polyacrylamide crosslinked with 2.75% methylenebisacrylamide, is described. This gel has a resolving power for very high molecular weight proteins and can be stained with silver. The gel is suitable for fractionation of factor VIII/von Willebrand factor directly from plasma samples. Visualization by silver staining revealed a series of covalently bound multimers with molecular weights of up to 8 X 10(6). The procedure described should be useful also for studies on other very high molecular weight proteins and nucleic acids.
D000178:Acrylamides; D004591:Electrophoresis, Polyacrylamide Gel; D005169:Factor VIII / Q000302:isolation & purification; D005340:Fibrinogen / Q000302:isolation & purification; D006801:Humans; D007075:Immunoglobulin M / Q000302:isolation & purification; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012834:Silver; D013194:Staining and Labeling; D014841:von Willebrand Factor / Q000302:isolation & purification
D000178:Acrylamides; D007075:Immunoglobulin M; D011506:Proteins; D014841:von Willebrand Factor; D012834:Silver; D005169:Factor VIII; D005340:Fibrinogen; C021221:N,N'-methylenebisacrylamide