The relationship between secreted and cell surface antigen-binding molecules synthesized by T cells. I. Function-related isotypic determinants on T cells defined by antisera to antigen-specific helper and suppressor factors.
Rabbit antisera have been used to define 'constant region' markers which distinguish between mouse T cell-derived helper and suppressor factors, regardless of their antigen-specificity or strain of origin. These antisera have also been shown to bind to functional T-cell lines. After several absorption steps, rabbit anti-helper factor serum bound specifically to mouse helper cell lines, whereas rabbit anti-suppressor factor serum bound specifically to suppressor cell lines. Neither antiserum bound to cytotoxic T cell lines. The 'isotypic' determinants defined by these antisera were demonstrated to be present on distinct subpopulations of non-transformed T cell populations, such as splenic T cells, cortisone-resistant thymocytes and Con A blasts, but were not found on Thy-1- spleen cells, bone marrow, brain, heart, liver, kidney or heart tissue. The antisera did not stain significant numbers of normal thymocytes, and so expression was restricted to mature T cells. T cells reactive with rabbit anti-helper factor serum were found in the Lyt 2- population of cortisone-resistant thymocytes, and constituted a major subpopulation of in vitro induced helper cells, while rabbit anti-suppressor factor serum stained cells found in the Lyt 2+ population of cortisone-resistant thymocytes, as well as the majority of in vitro induced suppressor cells. Thus, these antisera are potentially of great value in the definition and isolation of functionally-distinct T cell subpopulations.
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000950:Antigens, Ly; D000939:Epitopes; D007106:Immune Sera; D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions; D007375:Interleukin-1; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic