Polysomal and cytoplasmic mRNP particles containing 7S(L) RNA.
Nuclear RNP complexes, cytoplasmic mRNP particles and free and membrane-bound polysomes were prepared from rat liver and their low-molecular-mass RNA components were analyzed on polyacrylamide/formamide gels. The separated small RNAs transferred to diazophenylthioether paper were hybridized to the nick-translated recombinant plasmid pA6 containing cDNA sequences for the low-Mr RNA called 7S(L) RNA. Nuclear RNP particles and free and membrane-bound polysomes were found to contain 7S(L) RNA. In the cytoplasm 7S(L) RNA could be identified as the major small RNA in 20-S cmRNP particles.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis*; D004247:DNA; D007425:Intracellular Membranes / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000648:ultrastructure; D008297:Male; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012261:Ribonucleoproteins / Q000032:analysis*
D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D012313:RNA; D004247:DNA