Ultrastructural localization of monoclonal IgG antibodies for antigenic sites of Eimeria tenella oocysts, sporocysts, and sporozoites.
Monoclonal IgG antibodies against sporozoites of Eimeria tenella were obtained from the ascites fluid of BALB/c mice. Oocysts, sporocysts, and sporozoites were exposed to medium 199, normal ascites fluid, or monoclonal antibodies 1A, 9D, 3D3II, or 2G8f. Specimens were then incubated with ferritin-conjugated goat anti-mouse IgG antibody. Ferritin was uniformly distributed over the surface of sporozoites exposed to 1A, 9D, or 3D3II; ferritin was localized in patches on sporozoites exposed to 2G8f. A uniform layer of ferritin was present on the inner layer of oocyst walls and on the Stieda body, but not on the sporocyst wall. No ferritin was found on specimens exposed to medium 199 or normal ascites fluid. Monoclonal antibodies 1A, 9D, and 3D3II, but not 2G8F, caused complement-mediated lysis of sporozoites. These findings indicate that oocysts, sporocysts, and sporozoites of E. tenella contain common antigens specific for each monoclonal antibody tested.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D004539:Eimeria / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology*; D005293:Ferritins; D007074:Immunoglobulin G / Q000032:analysis; D007425:Intracellular Membranes / Q000276:immunology; D009940:Organoids / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D005293:Ferritins