Surface immunoglobulins as targets for anti-immunoglobulin-dependent cell-mediated lysis of B cells.
Human K cells are able to lyse human lymphoblastoid B cells in the presence of specific anti-human immunoglobulin isotype antibodies. The human lymphoblastoid B-cell line DAUDI (surface mu- and kappa-chains positive) was lysed in the presence of anti-mu and anti-kappa antisera, or IgG fraction of these antisera, and B-cell-depleted human lymphocytes. Lysis was not induced by anti-isotypic antisera alone or human lymphocytes alone. Lysis was not induced by antisera directed at isotypes which were not expressed on the DAUDI cells. The human lymphoblastoid B-cell line RAJI, which does not express surface membrane isotypes, could not be lysed by the anti-isotype-dependent cell-mediated mechanism. Lysis of DAUDI cells by this mechanism was mediated by Fc gamma receptor-bearing human non-B lymphocytes and required an intact Fc piece in the inducing anti-isotypic antibody. These observations are discussed as a possible role for K cells in the regulation of immune responses by interaction between anti-idiotypic antibodies and idiotype-bearing cells.
D000888:Antibodies, Anti-Idiotypic; D000920:Antibody-Dependent Cell Cytotoxicity; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes; D006801:Humans; D007111:Immunity, Cellular; D007142:Immunoglobulin gamma-Chains / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007142:Immunoglobulin gamma-Chains; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc