title: Purine metabolism in Leishmania donovani amastigotes and promastigotes.
abstract: Purine metabolism in Leishmania donovani amastigotes was found to be similar to that of promastigotes with the exception of adenosine metabolism. Adenosine kinase activity in amastigotes is approximately 50-fold greater than in promastigotes. Amastigotes deaminate adenosine to inosine through adenosine deaminase, an enzyme not present in promastigotes. Inosine is cleaved to hypoxanthine and phosphoribosylated by hypoxanthine-guanine phosphoribosyltransferase. Promastigotes cleave adenosine to adenine and deaminate adenine to hypoxanthine via adenase, an enzyme not present in amastigotes. Hypoxanthine is phosphoribosylated by hypoxanthine-guanine phosphoribosyltransferase.
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D007891:Leishmania / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D011684:Purine Nucleosides / Q000378:metabolism; D011685:Purine Nucleotides / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D013154:Spleen / Q000469:parasitology; D013997:Time Factors
chemical_list: D011684:Purine Nucleosides; D011685:Purine Nucleotides; D011687:Purines; D012313:RNA; D000241:Adenosine