title: A modified gel filtration technique producing an unusual exclusion volume of IgM: a simple way of preparing monoclonal IgM.
abstract: The vast majority of monoclonal IgM proteins is eluted just before the total volume of the column when filtered through G200 Sephadex or S200 Sephacryl gels equilibrated in a 0.005 M phosphate buffer but eluted with 0.05 M phosphate buffer containing 1.7 M NaCl. This unusual behaviour in low-ionic buffer is probably due to the poor solubility of IgM in diluted buffers. It allows a 1-step purification procedure under mild conditions and is suitable for both large and small scale preparations.
mesh_terms: D000180:Acrylic Resins; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002021:Buffers; D002850:Chromatography, Gel / Q000379:methods*; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000302:isolation & purification*; D009994:Osmolar Concentration
chemical_list: D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D002021:Buffers; D003911:Dextrans; D007075:Immunoglobulin M; C037239:Sephacryl Superfine; C025614:sephadex