Human serum IgE against two major allergens from cow hair and dander. Determination of affinity and quantity of antigen-specific IgE.
The quantity and quality of antigen-specific IgE against purified major allergens from cow hair and dander was measured by a double-antibody radioimmunoassay. In serum from patients with allergic asthma to cow the affinity constant varied from 1 to 150 X 10(9) l/mol and the number of antibody combining sites from 0.01 to 0.4 X 10-9 mol/l serum for each antigen.
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D000939:Epitopes; D006197:Hair / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000145:classification; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis
D000485:Allergens; D000939:Epitopes; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D007073:Immunoglobulin E