Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system.
Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS--polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same Mr-value as the native isoenzyme. CA III mRNA was found to represent approximately 0.55% of the total mRNA present in rat soleus muscle.
D000818:Animals; D002256:Carbonic Anhydrases / Q000096:biosynthesis*; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D009132:Muscles / Q000201:enzymology*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D011863:Radioimmunoassay; D051381:Rats
D012313:RNA; D002256:Carbonic Anhydrases