The carbohydrate on human chorionic gonadotropin produced by cancer cells.
Using the methods described, it is not possible to determine the number of N- and O-linked oligosaccharides on ectopic hCG beta. On standard hCG beta there are two NeuAc residues on each N- and O-linked oligosaccharide, so that the number of NeuAc residues is proportional to the number of oligosaccharides. Ectopic hCG beta and desialylated ectopic hCG beta are of similar molecular size to the standard preparations (gel filtration and RIA with anti-CTP antisera, data not presented). This suggests that ectopic hCG beta is sialylated to a similar extent as standard hCG beta, so the number of oligosaccharides on ectopic hCG beta could be similar to the number on standard hCG beta. There is a Fuc attached to the N-linked oligosaccharides of standard hCG beta (Fig. 3). Using the methods described, it was not possible to determine if this residue is also found on the N-linked oligosaccharides of ectopic hCG beta. Recently, a second form of ectopic hCG beta was identified (22). This form lacks the characteristic hCG beta carboxyterminal peptide, and as such is unrecognized by the RIA used in this study. Like the ectopic hCG beta described herein, and that produced by other cancers, this molecule only partially binds to Con A, and binds to Ricinus communis-120 following neuraminidase digestion. Intact hCG and free hCG subunits, which only partially bind to Con A, are found in cancer tissues, cancer sera, and the medium of cultured trophoblastic and nontrophoblastic cancer cells (Table 1). Our studies with DoT cancer of the cervix cells clearly indicate that the partial binding could be the consequence of the linkage of extra beta G1cNAc residues.
D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000378:metabolism*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism; D006729:Hormones, Ectopic / Q000032:analysis; D006801:Humans; D037102:Lectins; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D006729:Hormones, Ectopic; D037102:Lectins; D009844:Oligosaccharides; D010446:Peptide Fragments; D003208:Concanavalin A; D006026:Glycoside Hydrolases