Post-translational heterogeneity of the human vitamin D-binding protein (group-specific component).
The vitamin D-binding protein in human serum (the group-specific component) is an alpha 2-globulin which is genetically polymorphic in all populations studied. Previous work (J. Svasti and B. H. Bowman (1978) J. Biol. Chem. 253, 5188-5194, and J. Svasti, A. Kurosky, A. Bennett, and B. H. Bowman (1979) Biochemistry 18, 1611-1617) has shown that the electrophoretic variations of the proteins controlled by two allelic genes, Gc1 and Gc2, are due to at least three amino acid substitutions between Gc1 and Gc2 (Svasti et al. (1979] and to heterogeneity in the Gc1 phenotype arising from carbohydrate dissimilarities. Gc1 migrates electrophoretically as two protein bands, while Gc2 migrates cathodally as a single band. This study demonstrates a post-translational glycosylation difference occurring in a single area of the Gc1 sequence which accounts for the heterogeneity observed previously. The glycosylation site, a threonine residue, appears to be in a sequence which differs between Gc1 and Gc2. The O-glycosidic bond, which is typical of mucins, is rare in plasma proteins. The cyanogen bromide fragment containing the galactosamine-containing carbohydrate in Gc1 was partially sequenced through 20 residues from the amino terminus. No detectable galactosamine could be found in the homologous cyanogen bromide fragment in Gc2. A new purification procedure for the vitamin D-binding protein in human plasma has been developed. Three chromatographic steps provide purified protein.
D000510:Alpha-Globulins / Q000235:genetics*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011499:Protein Processing, Post-Translational; D014809:Vitamin D-Binding Protein
D000510:Alpha-Globulins; D000596:Amino Acids; D002352:Carrier Proteins; D014809:Vitamin D-Binding Protein