Efficient method for visualization and isolation of proteins resolved in polyacrylamide gels.
Polyacrylamide gel electrophoresis is a popular method used to purify proteins for reconstitution experiments, amino acid composition and sequence determinations. In this report we describe methods that will be of general use in the isolation and characterization of proteins and the benefits of substituting boric acid for glycine in the electrophoresis tray buffers. We also describe how proteins resolved in a variety of gel systems (including those containing sodium dodecyl sulfate) may be rapidly visualized with 8-anilino-1-naphthalene sulfonic acid and efficiently transferred to a second gel for two-dimensional gel analysis, or isolated by electroelution for subsequent characterization.
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification; D001888:Boric Acids; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000032:analysis; D006657:Histones / Q000302:isolation & purification; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling; D014337:Trout
D000817:Anilino Naphthalenesulfonates; D001798:Blood Proteins; D001888:Boric Acids; D006657:Histones; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C032688:boric acid; D005998:Glycine