Small RNAs from Drosophila KC-H cells. Characterization of nuclear and cytoplasmic 7S subspecies.
The metabolic characteristics and nuclear and cytoplasmic localization of Drosophila 7 S small RNAs were investigated. It was found that although most Drosophila small RNAs show electrophoretic mobilities similar to those of mammalian cells, distinct metabolic behaviour can be detected in three 7 S subspecies (d1, d3 and d4) which were found not to be class III RNAs as reported for mammalian 7 S RNAs. Drosophila d3 and d4 were found to be localized in the nucleus and to be very rapidly labelled minor subspecies turning over with a half-life of less than 6 h. Although 7 S-d1 migrates close to mammalian 7 S-K, it is a major subspecies in Drosophila, is localized mostly in the nucleus and turns over with a half-life of less than 10 h in contrast with mammalian 7 S-K which has been observed to be quite stable.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D003720:Densitometry; D004331:Drosophila melanogaster / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D012313:RNA / Q000032:analysis*; D012342:RNA, Small Nuclear
D012342:RNA, Small Nuclear; D003609:Dactinomycin; D012313:RNA