Cellular responses to bacterial lipopolysaccharide: LPS facilitates priming of antigen-reactive T cells.
The effect of bacterial lipopolysaccharide (LPS) on the capacity of mice to mount a specific T-cell response to a protein antigen was examined. Inoculation of mice with LPS and ovalbumin (OA) resulted in enhancement of the T-cell proliferative response to OA. This enhancement was manifested in vitro by an increase in magnitude and by a more rapid appearance of the response after challenge with OA. This enhancement was also shown because the latent periods for the antigen-specific responses were reduced to 3 days after inoculation with antigen plus LPS, as compared with 5 days after inoculation with antigen alone. Various mouse strains, including the C3H/HeJ and C57BL/10ScN strains, responded to the adjuvant action of LPS at the T-cell level. Results suggest that LPS exerts this adjuvant effect by facilitating clonal expansion of antigen-reactive T cells during priming.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D007700:Kinetics; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010047:Ovalbumin / Q000276:immunology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000939:Epitopes; D008070:Lipopolysaccharides; D010047:Ovalbumin