Dextran-induced lowering of plasminogen proactivator and functionally active high molecular weight kininogen in the rat.
Incubation of plasminogen-free rat citrated plasma with acetone (23% v/v) yielded enzyme preparations with high levels of plasminogen activator (PGA) and kininogenase (kallikrein), but with a low concentration of high molecular weight kininogen (HMWK) active as cofactor for kaolin-induced activation of factor XII. When benzamidine (4.0 mM) was present during acetone activation, a high yield of functionally active HMWK was obtained. Gel chromatography separated PGA into one high molecular weight fraction (HMW-PGA) without kininogenase and BAEe esterase activity, and one fraction (LMW-PGA) eluting together with plasma kallikrein. Injection of dextran (100 mg/kg intravenously) reduced the amount of LMW-PGA to 40%, without altering the concentration of HMW-PGA, and with only a small reduction of the kininogenase activity.
D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology*; D004950:Esterases / Q000097:blood; D007610:Kallikreins / Q000097:blood; D007704:Kininogens / Q000097:blood*; D008297:Male; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D001550:Benzamidines; D003911:Dextrans; D007704:Kininogens; D004950:Esterases; D007610:Kallikreins; D010960:Plasminogen Activators