Extensive labelling of injured insect neurons with seven different heme peptides.
Seven different heme peptides were used in neuronal uptake and labelling experiments in flies. The peptides were: catalase, lactoperoxidase, hemoglobin, horseradish peroxidase (HRP), myoglobin, cytochrome c and microperoxidase. All of these peroxidase active peptides were taken up by lesioned neurons and the markers spread throughout the entire cells resulting in a detailed labelling of their processes and cell bodies. Only HRP was taken up by intact neurons. Attempts were made to block axonal transport of HRP with colchicine, vinblastine and 2,4-dinitrophenol. These attempts were unsuccessful and it is proposed that HRP and the other six heme peptides testes are non-selectively diffusing through lesioned or damaged nerve cells in flies.
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D004175:Diptera / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*
D010455:Peptides; D006418:Heme; D006735:Horseradish Peroxidase