Inhibition of the O2- -generating NADPH oxidase of guinea-pig polymorphonuclear leukocytes by rabbit antibody to homologous liver NADPH-cytochrome c (P-450) reductase.
Rabbit antibody highly specific for guinea-pig liver NADPH-cytochrome c (P-450) reductase was found to inhibit dose-dependently the O2- -generating activity of the membrane fraction isolated from phorbol-myristate acetate-stimulated, homologous polymorphonuclear leukocytes. In addition, the antibody also could inhibit the NADPH-cytochrome c (Nitroblue tetrazolium) reductase separated from the membrane fractions and phagosomes of leukocytes by polyacrylamide gel electrophoresis or gel filtration on a Sephacryl S-300 column in the presence of 0.2% Triton X-100. These results demonstrate that the NADPH-cytochrome c reductase in the membrane fractions of leukocytes is antigenically cross-reactive with homologous liver NADPH-cytochrome c reductase, and also suggest that the enzyme of leukocytes participates in the respiratory burst.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002462:Cell Membrane / Q000201:enzymology; D000939:Epitopes / Q000276:immunology; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D009251:NADPH-Ferrihemoprotein Reductase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D009504:Neutrophils / Q000201:enzymology*; D011817:Rabbits / Q000276:immunology; D013481:Superoxides / Q000378:metabolism*
D000906:Antibodies; D000939:Epitopes; D013481:Superoxides; D009247:NADH, NADPH Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase; D019255:NADPH Oxidases