The effects of secretagogues of isolated human gastric glands.
The function of isolated human gastric glands has been studied in vitro by measuring the 14C-aminopyrine accumulation (RAP) in basal, unstimulated, conditions and after stimulation with different secretagogues. A microscale technique was used which enabled determinations of RAP in tissue obtained as gastroscopic biopsies. In addition, oxyntic-gland-containing mucosa was obtained at gastric resections for gastric or prepyloric ulcer disease. Histamine and cAMP derivative both induced maximal stimulation; RAP was approximately three times larger than in basal states. Carbachol induced a smaller but still significant stimulation. Pentagastrin did not increase RAP above the unstimulated level. Combinations of histamine and carbachol or pentagastrin did not induce a larger response than carbachol alone. The peak acid response to pentagastrin or betazole in vivo did not correlate with the maximum RAP in vitro.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000632:Aminopyrine / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology*; D004381:Duodenal Ulcer / Q000503:physiopathology; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D010418:Pentagastrin / Q000494:pharmacology; D013276:Stomach Ulcer / Q000503:physiopathology
D000632:Aminopyrine; D003994:Bucladesine; D006632:Histamine; D002217:Carbachol; D010418:Pentagastrin; D015056:1-Methyl-3-isobutylxanthine