Persistence of Ia antigen and antigen-presenting activity by cultured human monocytes.
Human peripheral blood monocytes cultured in vitro on plastic for up to 28 days supported the proliferation of antigen-specific, HLA-restricted T-cell blasts as well as freshly isolated blood mononuclear cells. Seventy-three percent of adhered monocytes were Ia antigen-positive by immunofluorescence when the cultures were initiated, this proportion fell to 70 +/- 13% after 28 days. The antigen-presenting function of cultured monocytes was inhibited by monoclonal anti-Ia antibody as compared with freshly isolated mononuclear cells. These results indicate that human monocytes retain their antigen-presenting function during maturation to macrophages in vitro.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D002478:Cells, Cultured; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000949:Histocompatibility Antigens Class II