Cellular responses to bacterial lipopolysaccharide: T cells recognize LPS determinants.
An in vitro proliferative system was used to assess the capacity of lipopolysaccharide (LPS) to induce a specific T-cell response in mice. Sensitized T cells were generated in vivo by subcutaneous inoculation with LPS. These T cells, which were purified on nylon wool columns, were stimulated to proliferate in vitro by LPS. Results of several lines of experimentation confirmed that the responding cells were T cells. Additional experiments indicated that sensitized T cells could distinguish between LPS and ovalbumin. Finally, it was found that genetic responsiveness to the biological effects of lipid A was required for full elicitation of LPS-induced T-cell proliferation. The data were interpreted to indicate that LPS interacted with, and stimulated, antigen-specific murine T cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D008070:Lipopolysaccharides / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D000939:Epitopes; D008070:Lipopolysaccharides