Time-dependent effects of alpha-amanitin on nuclear maturation and protein synthesis in mammalian oocytes.
The addition of alpha-amanitin to extrafollicular, cumulus-enclosed ovine oocytes at explantation inhibits meiotic maturation and prevents many of the changes in protein synthesis that normally accompany maturation. By contrast, these inhibitory effects are considerably reduced by either delaying the addition of the drug for 1-4 h or by denuding the oocytes of all associated cumulus cells at the onset of culture. The observations that the inhibitory effect of cordycepin on nuclear maturation is also time-dependent and cumulus-cell-dependent and that the oocyte is susceptible to cordycepin for longer than its sensitivity to alpha-amanitin are consistent with the differential effects of these drugs on RNA synthesis. It is concluded that a transcriptional event at the onset of maturation is essential for the initiation of those changes in protein synthesis required for the regulation of nuclear and cytoplasmic maturation. It is uncertain, however, whether this transcriptional event occurs within the cumulus cells or within the oocyte.
D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D010063:Ovum / Q000166:cytology*; D014176:Protein Biosynthesis; D012313:RNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D012756:Sheep; D013997:Time Factors
D000546:Amanitins; D012313:RNA; D007986:Luteinizing Hormone