Detection of human antinuclear antibodies on rodent omenta.
Detection of antinuclear antibodies in the sera of patients by immunofluorescence using mouse or rabbit omentum is recommended, because artifacts stemming from cryostat sectioning and thawing the tissue are eliminated and the advantage of tissue-bound evenly spread cells is preserved. Moreover, the technique is sensitive and permits reading on easily recognizable cell types.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D004247:DNA / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009852:Omentum / Q000032:analysis*; D012313:RNA / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011928:Raynaud Disease / Q000276:immunology; D012595:Scleroderma, Systemic / Q000276:immunology
D000974:Antibodies, Antinuclear; D012313:RNA; D004247:DNA