A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis.
Three ultrasensitive protein silver-staining methods have been compared with respect to the detection of tRNA in polyacrylamide gels. The method of Sammons (D. W. Sammons, L.D. Adams, and E.E. Nishizawa (1981) Electrophoresis 2, 135-141) has been shown to have remarkable sensitivity, with a detection limit of 0.3 ng tRNA/mm2, allowing the two-dimensional fractionation of submicrogram amounts of bulk tRNA. The application of this technique to developmental and differentiation problems and other areas where the amounts of nonradioactive tRNA available are limited is anticipated.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008099:Liver / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008832:Microchemistry; D012343:RNA, Transfer / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D012343:RNA, Transfer