Nucleotide sequence cleavage of guanine-modified DNA with aflatoxin B1, dimethyl sulfate, and mitomycin C by bleomycin and deoxyribonuclease I.
In guanine-modified DNA with aflatoxin B1, dimethyl sulfate, and mitomycin C, the nucleotide sequence cleavage of bleomycin or DNase I has been investigated using 32P-labeled DNA fragments. In contrast with cis-dichlorodiammine platinum (II) which induces intrastrand crosslinking of guanine N7 atoms, these mono-covalent modifiers did not give remarkable alteration of the DNA nucleotide cleavage mode by bleomycin and DNase I. From the viewpoint of combination drug, it is of interest that mitomycin C promoted the DNA cleavage activity of bleomycin.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D001483:Base Sequence; D001761:Bleomycin / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006147:Guanine; D016685:Mitomycin; D008937:Mitomycins / Q000378:metabolism*; D013463:Sulfuric Acid Esters / Q000378:metabolism*; D013464:Sulfuric Acids / Q000378:metabolism*
D000348:Aflatoxins; D008937:Mitomycins; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; D001761:Bleomycin; D016685:Mitomycin; D006147:Guanine; D004247:DNA; D016604:Aflatoxin B1; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; C007482:dimethyl sulfate