[Isolation of mastocytes from rat peritoneal fluid using continuous Ficoll gradients].
A method for mast cells purification from rat peritoneal fluid is described. The method consists of a continuous Ficoll gradient between 20 and 22,5% (w/v) Ficoll and the cells are obtained with an 88% retrieval. Purity and viability were of 95% and 97% respectively. The cells so purified were functional against the compound 48/80 and the spontaneous secretion value was less than 8%.
D000818:Animals; D001202:Ascitic Fluid; D002469:Cell Separation; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D005260:Female; D005362:Ficoll; D006636:Histamine Release / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D005362:Ficoll; D003189:p-Methoxy-N-methylphenethylamine
Purificación de mastocitos de fluido peritoneal de rata utilizando gradientes continuos de Ficoll.