title: Localization and identity of adenosine deaminase-positive cells in tissues of the young rat and calf.
abstract: Rabbit antibody to calf adenosine deaminase (ADA) was used to localize this enzyme in tissues of the young rat and calf by the immunoperoxidase method. The distribution patterns of ADA in most tissues were similar for both species. Within the thymus gland, the enzyme was strongly expressed predominantly in cortical lymphocytes. In the spleen and lymph nodes, most lymphocytes of T-cell areas stained weakly for ADA, whereas only a small number of ADA-positive cells were found in B-cell areas. Clumps of strongly ADA-positive mononuclear blastoid and plasma cells were observed in the medullary regions of lymph nodes, around peri-arteriolar lymphocyte sheaths and in the red pulp of the spleen, and in the lamina propria of the intestine. Double immunofluorescence staining studies in the rat showed that some of these blastoid cells contained both ADA and immunoglobulins and appeared to be plasmablasts. Strong staining for ADA was also found, in both the rat and calf, in as yet unidentified mononuclear blastoid cells in the interstitium of non-lymphoid organs (kidney, heart, lung), in endothelial cells of some arterioles and capillaries, and in Kupffer cells of the liver. In addition, ADA was strongly expressed in calf bile canaliculi. These studies define areas in rat and calf tissues which contain ADA-positive cells and provide a model system for investigations of the relationship between ADA and the function and development of these cells.
mesh_terms: D000243:Adenosine Deaminase / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005260:Female; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008198:Lymph Nodes / Q000201:enzymology; D008297:Male; D009700:Nucleoside Deaminases / Q000032:analysis*; D051381:Rats; D011912:Rats, Inbred ACI; D013154:Spleen / Q000201:enzymology; D013194:Staining and Labeling; D013950:Thymus Gland / Q000201:enzymology
chemical_list: D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase