Development of a radioimmunoassay for pregnancy-associated plasma protein A and establishment of normal levels in the first trimester of pregnancy.
An accelerated double antibody method has been developed for the radioimmunoassay of pregnancy-associated plasma protein A (PAPP-A) in serum. The workable range for the assay was 0.04-1.8 mg/l of serum. PAPP-A levels were determined in single serum samples from 110 women with prospective normal pregnancies of between 7 and 14 weeks' gestation. The level of pregnancy specific beta 1 glycoprotein (SP1) was also measured in these samples and normal ranges for PAPP-A and SP1 were constructed from the results obtained.
D005260:Female; D006801:Humans; D008297:Male; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011261:Pregnancy Trimester, First; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis*; D011863:Radioimmunoassay; D012016:Reference Values
D011257:Pregnancy Proteins; D011266:Pregnancy-Associated Plasma Protein-A