[Embryonic hemoglobins in mammals: sequences of zeta-, epsilon- and theta chains in domestic pigs (Sus scrofa domestica)].
The amino-acid sequences of all expressed hemoglobins of the pig embryo are given: Hemoglobin Gower I (zeta 2/epsilon 2), Hemoglobin Gower II (alpha 2/epsilon 2), Hemoglobin Heide I (zeta 2/theta 2) and Hemoglobin Heide II (alpha 2/theta 2). The zeta-, epsilon- and theta-chains were obtained with chromatography on CM-cellulose from isolated hemoglobin components. The primary structure was established by sequencing the tryptic peptides in the sequenator: they were isolated using HPLC. The zeta-chains from pig and human differ in 23, the epsilon-chains in 20 positions. The embryonic globin-gene which express the theta-chains, is a new one in mammals, of epsilon-type and up to now it could only be found in pigs: the amino-acid sequence differ in only 4 positions from the epsilon-chains. Because no gamma-chains (fetal Hb) are expressed the sequences of all hemoglobins (5 hemoglobin chains forming 5 different hemoglobins) of ontogeny in pig are now described.
D000595:Amino Acid Sequence; D000818:Animals; D004622:Embryo, Mammalian; D005319:Fetal Hemoglobin; D046911:Macromolecular Substances; D013552:Swine / Q000097:blood*
D046911:Macromolecular Substances; D005319:Fetal Hemoglobin
Embryonale Hämoglobine der Säuger: Die Sequenzen der zeta-, epsilon- und theta-Ketten vom Hausschwein (Sus scrofa domestica).