[Cytomorphological effect of anticancer drugs on human solid tumors in nude mice--time response].
To evaluate the time response of anticancer drugs, we observed the morphological changes of solid tumor cells using a new simple method of cytologic examination. Mitomycin C and Bleomycin were administered to nude mice transplanted with human uterine cervical carcinoma of the Yumoto strain. The cancer cells were classified as follows: A, uniform, pyknotic nuclei with indistinct nucleoli; B, pyknotic nucleus with distinct nucleoli; C, pale-stained nuclei and cytoplasm; D, condensation of nuclei and cytoplasm. Three hours after MMC administration the destruction of the nuclei of A type cancer cells and shrinkage of B type cells was observed. Pyknosis of B type cells and destruction of C type cells was seen 7 hours after BLM administration. These characteristic changes which occurred at a very early period appear to be available as a marker for the evaluation of the oncostatic effect and for the anticancer drug selection in patients with malignancy.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D002467:Cell Nucleus / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009368:Neoplasm Transplantation; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology*
D000970:Antineoplastic Agents; D008937:Mitomycins; D001761:Bleomycin; D016685:Mitomycin