title: The enhancement of specific gene transcription in isolated nuclei by added HeLa whole cell extract.
abstract: Transcription was carried out in isolated rat liver nuclei by endogenous RNA polymerase with the addition of HeLa whole cell extract (HWCE) in the presence of nucleoside 5'-[gamma-S]triphosphate. The resulting 5'-gamma-thiophosphate on the synthesized RNA allows separation of in vitro initiated RNA from bulk RNA by mercury-agarose chromatography. HWCE not only increased initiation of new RNA chains greater than 3 times but also had no effect on the RNA synthesis by RNA polymerase II. The initiation of transcription of albumin and alpha-fetoprotein genes in an isolated nuclei system was selectively enhanced by HWCE. Using this system, we studied the effect of glucocorticoid on albumin and alpha-fetoprotein gene expression in vitro.
mesh_terms: D000418:Albumins / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D002474:Cell-Free System; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells / Q000502:physiology*; D006801:Humans; D008970:Molecular Weight; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D014158:Transcription, Genetic; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D000418:Albumins; D000509:alpha-Fetoproteins; D012313:RNA; D003907:Dexamethasone