Metabolic differentiation of homologous leg muscles of two aquatic birds at the level of enzymatic organization.
The quantitative determination of succinic dehydrogenase (SDH), hexokinase (HK), phosphorylase, phosphofructokinase (PFK), glycerol-3-phosphate dehydrogenase (G-3-PDH) and lactate dehydrogenase (LDH) was carried out in the homologous leg muscles of two aquatic Birds. It appears that the leg muscle fibres of the coot, a surface swimmer are more oxidative in nature and appear to utilize glucose as source of energy. The leg muscles of the dabchick, a diving Bird, on the other hand, seem to depend on glycogen as source of energy. The relative activity levels of HK, phosphorylase and PFK support the accepted rôle of glycogen as primary substrate of carbohydrate catabolism in the leg muscles. The ratio of G-3-PDH/LDH in the leg muscles revealed that glycerol 3-phosphate cycle appears to be insufficient to account for the major part of NADH oxidation. However, the LDH activity is quite high in all the muscles. These results led us to believe that glycerol 3-phosphate cycle may function during rest, when the rate of glycolysis will be low.
D000818:Animals; D001717:Birds / Q000502:physiology*; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D006019:Glycolysis; D006593:Hexokinase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D009132:Muscles / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000032:analysis; D006005:Phosphorylases / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis; D013550:Swimming; D014018:Tissue Distribution
D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D006005:Phosphorylases; D006593:Hexokinase; D010732:Phosphofructokinase-1