The transport and processing of carbamyl phosphate synthetase-I in mouse hepatic mitochondria.
Carbamyl phosphate synthetase-I (CPS-I)2, purified from mouse hepatic mitochondria consists of electrophoretically homogeneous polypeptide species of 160 kilodaltons molecular weight (Kd). Monospecific antibody to CPS-I immunoprecipitated a putative precursor of 165Kd protein from in vitro translation products programmed with mouse liver free polysomes or poly(A) RNA. Isolated mitochondrial particles can take up and process pCPS-I into mature CPS-I of 160Kd in an in vitro transport system. The in vitro transport of CPS-I is energy dependent and requires intact mitochondria. The processing of pCPS-I appears to involve a single endoproteolytic cleavage.
D000818:Animals; D001692:Biological Transport; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics* / Q000302:isolation & purification; D008025:Ligases / Q000235:genetics*; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology*; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D008025:Ligases; D002222:Carbamoyl-Phosphate Synthase (Ammonia)