An electrophoretic method for the identification of poly-gamma-glutamyl chain lengths in folates and related compounds.
An electrophoretic method for the identification and separation of folyl polyglutamates of different chain lengths and of the corresponding p-aminobenzoyl polyglutamate compounds has been developed. These compounds have been separated using electrophoresis in a 40% polyacrylamide gel using a higher voltage and other modifications of the standard polyacrylamide gel electrophoresis procedures used to separate larger polypeptides. Good separation has been obtained on folates containing up to 12 glutamyl residues. Further, this method has been used to investigate the nature of the products formed by the gamma-glutamyl carboxypeptidases from hog kidney and bovine liver.
D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005492:Folic Acid / Q000031:analogs & derivatives*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000032:analysis; D011624:Pteroylpolyglutamic Acids / Q000032:analysis*; D013552:Swine; D011623:gamma-Glutamyl Hydrolase / Q000378:metabolism*
D011624:Pteroylpolyglutamic Acids; D011099:Polyglutamic Acid; C027346:4-aminobenzoyl-poly-gamma-glutamate; D005492:Folic Acid; D002268:Carboxypeptidases; D011623:gamma-Glutamyl Hydrolase