title: Poly(A)+ RNA metabolism during change of physiological state of Tetrahymena pyriformis cells.
abstract: In the present work the metabolism of poly(A)+ RNA was investigated in cells of Tetrahymena pyriformis derived either from stationary cultures or from starved suspensions that were initiating growth. Under these circumstances the organisms derived from stationary cultures synthesize ribosomal and poly(A)+ RNA and form polysomes. In the presence of actinomycin D (actD) the observed expansion of the polysomal population is arrested. Pre-starved cells, on the other hand, start making polysomes in the virtual absence of ribosomal and poly(A)+ RNA synthesis soon after being transferred to peptone medium. In this case polysome formation is only partially sensitive to actD. These results have been interpreted as indicating that, in the beginning of growth, cells derived from stationary cultures are dependent on RNA synthesis for polysome formation, whereas pre-starved cells use pre-synthesized RNA for the same purpose.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology; D011061:Poly A / Q000096:biosynthesis / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D003609:Dactinomycin; D011061:Poly A; D012313:RNA