[Intercellular information transfer in the immunogenesis process. VIII. Analytical separation on agar and polyacrylamide gel of RNA fractions from the spleens of immunized and intact rats].
RNA isolated from the spleens of intact rats and from rats with immunized sheep red cells was fractionated through three steps: 1 - extraction from phenol nuclei at 50-55 degrees and 65-75 degrees C, 2 - calcium-phosphate chromatography, 3 - agar electrophoresis. Eight agar fractions were obtained of the spleens of immunized rats, an increased RNA content was manifested in at least three agar fractions: the first (4 S), the third (21 S) and the eighth (26 S) ones. The first and the eighth immune RNA fractions, as it was shown earlier, induce the synthesis of antibodies in the rat transplantable lymphosarcoma cell. The first agar fraction of nuclear RNA from the spleens of immunized and intact rats were additionally separated using PAAG electrophoresis. The 4 S agar RNA fraction appears to be rather heterogeneous. It contains 4 S, 4.5 S, 5 S, 5.8 S, U1, U2 and 8 SII fractions, which are low-molecular nuclear RNAs, the 4 S subfraction prevailing. It is suggested that the 4 S PAAG subfraction is most active in the synthesis of antibodies induced by the heterogeneous agar 4 S RNA.
D000818:Animals; D000917:Antibody Formation; D002450:Cell Communication; D002467:Cell Nucleus / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology; D007114:Immunization; D012313:RNA / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology*
D012313:RNA
O mezhkletochnoĭ peredache informatsii v protsesse immunogeneza. VIII. Analiticheskoe razdelenie v agare i poliakrilamidnoĭ gele nekotorykh fraktsiĭ RNK iz selezenok immunizirovannykh i intaktnykh krys.