An improved method for increasing the resolution and sensitivity of silver staining of nucleic acid bands in polyacrylamide gels.
Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D012313:RNA / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D012834:Silver; D013194:Staining and Labeling
D012329:RNA, Bacterial; D012834:Silver; D012313:RNA; D012343:RNA, Transfer