title: Genes and pseudogenes for mouse U1 and U2 small nuclear RNAs.
abstract: Regions of the mouse genome homologous to U1 and U2 small nuclear RNAs have been isolated. The U1 regions are identified as pseudogenes on the basis of: 1) insertions, deletions, and substitutions in the sequence of the DNA as compared with that of U1 RNA; 2) absence of complementary RNA in mouse tissues; and 3) lack of expression upon microinjection in Xenopus oocyte nuclei. A U2 region is identified as a gene on the basis of near identity of sequence with U2 RNA and capacity to direct the synthesis of U2-like RNA in Xenopus oocyte nuclei. Both U1 and U2 sequences are repeated in the mouse genome, the U2 sequences analyzed here in an inverted repeat arrangement.
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D005260:Female; D005796:Genes; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000378:metabolism; D012313:RNA / Q000235:genetics*; D012342:RNA, Small Nuclear; D014981:Xenopus
chemical_list: D004274:DNA, Recombinant; D012342:RNA, Small Nuclear; D012313:RNA