Effect of cytochalasin D on secretion by rat pancreatic acini.
Using the model of isolated acini the effect of cytochalasin D (CD) on rat pancreatic secretion in vitro was studied. The influence of CD (0.01-10 micrograms/ml = 0.02-19.7 microM) on amylase secretion and 3H-pulse-labelled protein release was measured under two sets of conditions: (a) basal, and (b) stimulated by 77pM caerulein. Basal secretion was not altered, but stimulated secretion of amylase and 3H-labelled proteins were similarly inhibited by up to 45%. It is concluded that CD affects only exocytosis of zymogen granules and not intracellular transport.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors; D005089:Exocytosis / Q000187:drug effects; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014617:Vacuoles / Q000648:ultrastructure
D003572:Cytochalasins; D004792:Enzyme Precursors; D011506:Proteins; D015638:Cytochalasin D; D000681:Amylases