Histochemical localization of neuraminidase in the CNS of mice and fish by means of 5-brom-3-indolyl-alpha-ketoside of 5-N-acetyl-D-neuraminic acid (BI-NeuAc).
The applicability of the 5-brom-3-indolyl-alpha-ketoside of N-acetyl-D-neuraminic acid (BI-NeuAc) as substrate for the histochemical indication of neuraminidase (GOSSRAU et al. 1977) was examined in frozen sections of brain, small intestine and kidney from suckling and adult mice and respectively from a cichlid fish (Sarotherodon mosambicus) with and without formaldehyde fixation. Following biochemical investigations using tritiated gangliosides as substrate a decrease in the activity of neuraminidase took place from about 50 to 80% after tissue fixation with formaldehyde. Nevertheless significant histochemical reactions were found only in fixed tissue sections. The specificity of this reaction was shown by means of inhibitions tests using 2,3-dehydro-2-desoxy-N-acetyl-neuraminic acid according to KUMAR et al. (1981). In contrast to distinct staining of neuraminidase activity in the small intestine and kidney, only faint reactions occurred in brain sections. From this it is concluded that the application of the new synthetic substrate for quantitative staining of neuraminidase seems to be not very suitable for the CNS.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D005399:Fishes; D006651:Histocytochemistry; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008297:Male; D051379:Mice; D009439:Neuraminidase / Q000032:analysis*; D012794:Sialic Acids; D013045:Species Specificity; D013194:Staining and Labeling
D012794:Sialic Acids; C039600:5-N-acetylneuraminic acid-5-bromo-3-indolyl-alpha-ketoside; D009439:Neuraminidase