Functional properties of T cell clones with a double specificity for alloantigens and foreign antigens.
Four keyhole limpet hemocyanin (KLH)-specific clones prepared from the lymph node of CB6F1 mice immunized with KLH had a proliferative response restricted to parental major histocompatibility complex (MHC)-encoded antigens. These clones provided help for CB6F1 trinitrophenyl-ovalbumin (TNP-OVA)-primed B cells to mount IgM and IgG plaque-forming cell (PFC) responses in the presence of KLH-TNP conjugate. In addition, two of these clones (A12.11 and F6) proliferated in response to allogeneic cells from mice strains bearing H-2k or H-2q haplotypes, respectively. However, they did not provide help for C3H/He or B10.Q primed B cells. The clonal nature of A12.11 and F6 was demonstrated by subcloning and in BUdR-suicide experiments. The proliferative response to KLH was ablated by anti-Iad antibodies, whereas the proliferation induced by C3H/HeJ stimulating cells was ablated by anti-Iak antibodies. Furthermore, both responses were inhibited by a monoclonal anti-clonotype (idiotype) antibody. Taken together, these results strongly support the hypothesis that the same receptor recognizes alloantigens and KLH associated with self-antigens.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006433:Hemocyanins / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D008027:Light; D008213:Lymphocyte Activation; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000961:Antilymphocyte Serum; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D001973:Bromodeoxyuridine