title: Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function.
abstract: Simian virus 40 (SV 40) stimulated a host cell antigen in the centriolar region after infection of African green monkey kidney (AGMK) cells. The addition of puromycin and actinomycin D to cells infected with SV40 within 5 h after infection inhibited the stimulation of the host cell antigen, indicating that de novo protein and RNA syntheses that occurred within the first 5 h after infection were essential for the stimulation. Early viable deletion mutants of SV40 with deletions mapping between 0.54 and 0.59 map units on the SV40 genome, dl2000, dl2001, dl2003, dl2004, dl2005, dl2006, and dl2007, did not stimulate the centriolar antigen above the level of uninfected cells. This indicated that an intact, functional small-t protein was essential for the SV40-mediated stimulation of the host cell antigen. Our studies, using cells infected with nondefective adenovirus-SV40 hybrid viruses that lack the small-t gene region of SV40 (Ad2+ND1, Ad2+ND2, Ad2+ND3, Ad2+ND4, and Ad2+ND5), revealed that the lack of small-t gene function of SV40 could be complemented by a gene function of the adenovirus-SV40 hybrid viruses for the centriolar antigen stimulation. Thus, adenovirus 2 has a gene(s) that is analogous to the small-t gene of SV40 for the stimulation of the host cell antigen in AGMK cells.
mesh_terms: D000818:Animals; D000941:Antigens; D000956:Antigens, Viral / Q000235:genetics; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D002502:Centrioles / Q000276:immunology*; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D009940:Organoids / Q000276:immunology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000502:physiology*
chemical_list: D000941:Antigens; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D012313:RNA