Inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-beta-D-arabinofuranosyladenine 5'-monophosphate (ara-AMP) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-GMP).
The thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) is associated with the following enzyme activities:ATP:dThd (dCyd) deoxypyrimidine kinase, ATP:dTMP thymidylate kinase, ADP:dThd- and AMP:dThd 5'-phosphotransferase. In kinetic experiments it is shown that ara-AMP inhibits AMP:dThd- and ADP:dThd phosphotransferase activity, while acyclo-GMP impairs ADP:dThd phosphotransferase reaction only; the inhibition was found to be non-competitive. The functional subunit ATP:dThd kinase was not affected by either compound.
D000212:Acyclovir / Q000031:analogs & derivatives* / Q000494:pharmacology; D001088:Arabinonucleotides / Q000494:pharmacology*; D007700:Kinetics; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors*; D001084:Vidarabine Phosphate / Q000494:pharmacology*
D001088:Arabinonucleotides; D009097:Multienzyme Complexes; D001084:Vidarabine Phosphate; C026145:acyclovir monophosphate; D013937:Thymidine Kinase; D000212:Acyclovir