Monoclonal anti-cardiolipin antibodies bind to DNA.
BALB/c mice immunized with the phospholipid, cardiolipin, produced anti-cardiolipin and anti-DNA antibodies. Seven hybridomas derived from spleen cells of the cardiolipin-immunized mice produced cardiolipin-binding monoclonal antibodies that also bound to the polynucleotides DNA, poly(dT), and poly(I). The seven cardiolipin-induced monoclonal antibodies shared idiotypic determinants with a high frequency idiotypic marker of spontaneously expressed anti-DNA autoantibodies of lupus-prone MRL-lpr/lpr mice. The monoclonal antibodies presumably bound to phosphodiester phosphate groups that occur in both polynucleotides and phospholipids. The results imply that production of anti-DNA autoantibodies does not require immunization by DNA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology*; D003429:Cross Reactions; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes; D005260:Female; D007130:Immunoglobulin Idiotypes; D051379:Mice; D009691:Nucleic Acid Denaturation
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D002308:Cardiolipins; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D004247:DNA