title: Surface charges associated with fenestrated brain capillaries. I. In vitro labeling of anionic sites.
abstract: Ferritin derivatives with different pI values and the basic dye ruthenium red have been used as cationic probes to localize anionic sites associated with fenestrated brain capillaries. Cationic ferritin was found in the endothelial basement membrane and the basement membrane of the perivascular cellular linings in amounts far exceeding those observed with anionic derivatives, the degree being greater for the more cationized ferritin molecules. Labeling of the luminal endothelial front with cationic ferritin was only achieved when a serum- or albumin-free medium was applied. Furthermore, the striated collagen fibers were coated with cationic ferritin molecules in a highly ordered fashion. Ruthenium red localized to the same sites. The findings suggest the existence of a perivascular charge filter around fenestrated capillaries of the brain. Some physiological roles of this filter are discussed, as related to its possible function in regulating homeostasis of cerebrospinal fluid.
mesh_terms: D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D000838:Anions; D001485:Basement Membrane / Q000378:metabolism; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000378:metabolism; D002412:Cations; D004563:Electrochemistry; D004727:Endothelium / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism; D007526:Isoelectric Point; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000345:Affinity Labels; D000838:Anions; D002412:Cations; D012430:Ruthenium Red; D005293:Ferritins