Detection of reverse transcriptase activity in association with the non-A, non-B hepatitis agent(s).
Particle-associated reverse transcriptase activity was detected in four human serum specimens and in two plasma-derived products, all of which had been shown to transmit non-A, non-B hepatitis (NANBH) to other human beings and/or chimpanzees. Reverse transcriptase activity was also detected in all twelve sera from patients with acute or chronic NANBH. In contrast, reverse transcriptase activity was found in only 2 of 49 serum specimens from healthy plasma donors and laboratory workers. Sucrose density gradient fractions of two of the infectious human sera (peak reverse transcriptase activity at 1.14 g/ml) transmitted NANBH to chimpanzees. Biochemical and enzymatic data indicate that the NANBH agent(s) is a retrovirus or is retrovirus-like.
D000818:Animals; D002499:Centrifugation, Density Gradient; D006526:Hepatitis C / Q000201:enzymology* / Q000382:microbiology; D006525:Hepatitis, Viral, Human / Q000201:enzymology*; D006801:Humans; D002679:Pan troglodytes; D012194:RNA-Directed DNA Polymerase / Q000097:blood*; D014771:Virion
D012194:RNA-Directed DNA Polymerase