Activation of macrophages by substance P: induction of oxidative burst and thromboxane release.
Substance P (SP), a putative neuropeptide transmitter, was examined for its effects on macrophages. Guinea-pig peritoneal macrophages were purified by adherence, challenged with SP and incubated for up to 18 h. Culture supernatants were collected to determine the release of O-2, H2O2 and TXB2. SP dose-dependently evoked O-2 and H2O2 production by C. parvum-activated macrophages and liberation of TXB2 from albumin-elicited macrophages. Generation of these proinflammatory macrophage products by SP may be relevant in the context of neuroinflammatory disease.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D013373:Substance P / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000378:metabolism*; D013931:Thromboxanes / Q000378:metabolism*
D001095:Arachidonic Acids; D013931:Thromboxanes; D013373:Substance P; D013929:Thromboxane B2; D006861:Hydrogen Peroxide