Fluorescence polarization study of tertiary structure of DNA within bacteriophage lambda.
The fluorescence polarization of acridine orange-stained, oriented lambda phages was measured. The parameters of DNA packing within the phage head cos2 theta and cos4 theta were calculated (theta, angle between the direction of a small segment of DNA and the phage axis). It is shown that simple models of lambda phage DNA tertiary structure are not consistent with calculated values. A new model is proposed.
D000165:Acridine Orange; D010582:Bacteriophage lambda / Q000235:genetics*; D004279:DNA, Viral; D004563:Electrochemistry; D005454:Fluorescence Polarization; D008433:Mathematics; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D013194:Staining and Labeling
D004279:DNA, Viral; D000165:Acridine Orange