Characterization of cyclophosphamide-induced suppressor cells.
Spleen cells from mice injected with cyclophosphamide (200 mg/kg body weight) suppress the secondary immunoglobulin (IgG antibody response of memory cells to a T-dependent antigen, dinitrolphenylated (DNP) human gamma globulin, in Millipore diffusion chambers. Characterization of the suppressor cell revealed that it was nylon wool nonadherent, insensitive to treatment with anti-Thy 1.2 or anti-Ig serum plus C, found in spleen but not thymus or lymph nodes, generated in thymectomized mice and nude mice, and heat-sensitive. Because of these unique characteristics, it was concluded that the suppressor cell was not a typical B cell, T cell, or macrophage. The suppressor cell was found to be regulated, i.e., inhibited, by a T cell found in the thymus and possibly the lymph nodes of normal mice. The suppressor cells may have biological significance in relation to development of self-tolerance.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D006462:Hemolytic Plaque Technique; D007104:Immune Adherence Reaction; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D009757:Nylons; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D013950:Thymus Gland / Q000502:physiology; D005719:gamma-Globulins / Q000276:immunology
D007074:Immunoglobulin G; D009757:Nylons; D005719:gamma-Globulins; D003520:Cyclophosphamide