Inhibition of cell growth by irradiation in combination with hyperthermia or anticancer drugs.
Using a clonogenic assay and the human bladder cancer cell line T24, enhanced cytotoxicity by the combined use of irradiation and 43 degrees C hyperthermia or two anticancer drugs, bleomycin (BLM) and cis-dichlorodiammineplatinum (CDDP), has been investigated in vitro, as well as the size of colonies derived from single survivor cells after treatment. Irradiation combined with 43 degrees C hyperthermia showed greatly enhanced cytotoxicity and diminution in mean colony diameter and decreased distribution of colony size. BLM had almost the same effect as 43 degrees C hyperthermia in terms of cytotoxicity and colony size. While CDDP showed a certain degree of enhanced cytotoxicity in combination with irradiation, it produced no appreciable decrease in mean colony diameter. Hyperthermia may be beneficial in the management of radiation resistant cells surviving a large dose of radiation, and would seem to be a promising means for achieving eradication of cancer cells in combination with radiation therapy.
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001761:Bleomycin / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002945:Cisplatin / Q000627:therapeutic use; D003131:Combined Modality Therapy; D006801:Humans; D006979:Hyperthermia, Induced; D014410:Tumor Stem Cell Assay; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*
D000970:Antineoplastic Agents; D001761:Bleomycin; D002945:Cisplatin