Development and use of radioimmunoassays for the detection of human IgG antibodies to laboratory animal urinary proteins.
A radioimmunoassay method for the detection of human IgG antibodies to laboratory animal urinary proteins has been developed using partially purified protein fractions prepared from crude urine extracts by gel filtration. The proteins from the four species, mouse, rat, guinea pig and rabbit, were labelled with 125I and used in an antigen binding assay employing a sepharose-linked staphylococcal protein A immunosorbent. Results, expressed as arbitrary units of specific IgG per unit serum, show antibodies to one or more species present in 57% of current animal workers compared with 6% of non-animal workers.
D000818:Animals; D001667:Binding, Competitive; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D000939:Epitopes; D006168:Guinea Pigs; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009784:Occupational Diseases / Q000175:diagnosis / Q000276:immunology; D011507:Proteinuria / Q000175:diagnosis* / Q000276:immunology; D011817:Rabbits; D011852:Radioallergosorbent Test; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D000939:Epitopes; D007074:Immunoglobulin G