Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine.
One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.
D002855:Chromatography, Thin Layer; D010768:Phosphoserine / Q000302:isolation & purification*; D010769:Phosphothreonine / Q000302:isolation & purification*; D019000:Phosphotyrosine; D012694:Serine / Q000031:analogs & derivatives*; D013912:Threonine / Q000031:analogs & derivatives*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D013912:Threonine; D014443:Tyrosine; D012694:Serine