title: Antiviral and antiproliferative effects of interferons delivered via monoclonal antibodies.
abstract: We present in vitro experiments demonstrating the feasibility of specifically targeting IFNs by coupling to monoclonal antibodies (MAbs). Human interferon alpha (HuIFN-alpha), both natural and recombinant, were coupled to Epstein-Barr virus (EBV) membrane antigen (MA) specific MAbs and shown to retain full antiviral and antiproliferative activities when tested on several human cell lines. A series of in vitro pulsing experiments demonstrated that a minimum amount of MAb-delivered IFN exerts pronounced antiviral and antiproliferative effects on human QIMR-WIL target cells which carry EBV (viral) membrane antigens (EBV-MA+) and not on U-266 or Hep2 cell lines (EBV-MA-). Additionally the antiproliferative action of MAb-coupled rIFN-alpha appeared to be enhanced compared with that of uncoupled.
mesh_terms: D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000914:Antibodies, Viral / Q000008:administration & dosage; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000008:administration & dosage*; D014762:Viral Interference
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007372:Interferons