Crystallization of a ribonuclease-resistant fragment of Escherichia coli 5 S ribosomal RNA and its complex with protein L25.
A ribonuclease-resistant fragment of Escherichia coli 5 S ribosomal RNA has been crystallized. The space group is P6(1)22 or P6(5)22, with a = 59.5 A and C = 268 A. The crystals contain one molecule per asymmetric unit, and show diffraction to 4.0 A resolution. Also, a complex of this fragment with L25 ribosomal protein has been crystallized in the same space group, but with a = 119 A, c = 250 A and four molecules per asymmetric unit.
D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D046911:Macromolecular Substances; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C031127:ribosomal protein L25