Measurement of 3-methoxy-4-hydroxyphenylacetic acid (HVA) in plasma by high-performance liquid chromatography with electrochemical detector (HPLC-EC).
A simple and sensitive high-performance liquid chromatography method with electrochemical detector is described for the determination of free 3-methoxy-4-hydroxyphenylacetic acid (HVA) in human plasma. The method does not involve any extraction, is specific and reproducible, and has the potential to measure serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) simultaneously. The plasma concentration of free HVA in eight normal, healthy adult volunteers was 10.9 +/- 4.6 (mean +/- SD). In a preliminary study, in one schizophrenic patient the plasma HVA increased twofold after neuroleptic treatment.
D000818:Animals; D014150:Antipsychotic Agents / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D006719:Homovanillic Acid / Q000097:blood*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D008297:Male; D010648:Phenylacetates / Q000097:blood*; D051381:Rats; D012559:Schizophrenia / Q000097:blood / Q000188:drug therapy; D012701:Serotonin / Q000097:blood
D014150:Antipsychotic Agents; D010648:Phenylacetates; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid