Detection and affinity purification of beta-endorphin precursors using a monoclonal antibody.
A monoclonal antibody to porcine beta-lipotropin has been produced which binds to the N-terminal (gamma-lipotropin) portion of the molecule. The antibody can be used to detect beta-lipotropin as well as other beta-endorphin precursors (predominantly a Mr 38 000 polypeptide) using radiobinding assay or the immunoblotting technique. Purification of the peptides can be readily achieved by affinity chromatography using the monoclonal antibody covalently bound to Sepharose 4B. As the antibody recognises the N-terminal part of beta-lipotropin, it can be used to detect and purify beta-lipotropin and other beta-endorphin precursors in the presence of beta-endorphin.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity; D004723:Endorphins / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010902:Pituitary Gland / Q000032:analysis; D010908:Pituitary Hormones, Anterior / Q000032:analysis; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors / Q000032:analysis*; D013552:Swine; D001615:beta-Endorphin; D008083:beta-Lipotropin / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004723:Endorphins; D000939:Epitopes; D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; D008083:beta-Lipotropin