Insect sex chromosomes. VIII. Identification of active/inactive X-chromosomes in Gryllotalpa fossor by 5-BrdU/AO fluorescence.
By employing 5-BrdU/AO fluorescence technique to distinguish active from inactive X-chromosomes, we have, for the first time, provided evidence in support of the validity of this technique for a non-mammalian system Gryllotalpa fossor (Orthoptera). In the female somatic cells of Gryllotalpa, it is only in the active (euchromatic) arm of X-chromosome that the fluorescence is bright, whereas it is dull in the facultative and constitutive heterochromatic arms. In tetraploid spermatogonial cells one of the two X-chromosomes is inactive, suggesting that this phenomenon is probably a random one and that the inactivation process is independent of the imprinting mechanism.
D000165:Acridine Orange; D000818:Animals; D001973:Bromodeoxyuridine; D005260:Female; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008856:Microscopy, Fluorescence; D009987:Orthoptera / Q000648:ultrastructure*; D012730:Sex Chromosomes / Q000502:physiology*; D013093:Spermatogonia / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods*; D014960:X Chromosome / Q000502:physiology*
D000165:Acridine Orange; D001973:Bromodeoxyuridine