Depolarizing agents regulate the phosphorylation of myelin basic protein in rat optic nerves.
The regulation of the state of phosphorylation of myelin basic protein has been studied in intact rat optic nerves incubated in vitro. For this purpose the endogenous state of phosphorylation was preserved and the "back-phosphorylation" technique was used to determine the amount of dephosphorylated protein present in extracts of the nerves. Our results indicate that when nerves were incubated in the presence of depolarizing agents, the state of phosphorylation of myelin basic protein was increased. This effect was calcium-dependent and was partly inhibited by chlorpromazine.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D004676:Myelin Basic Protein / Q000302:isolation & purification / Q000378:metabolism*; D009900:Optic Nerve / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D004676:Myelin Basic Protein; D000255:Adenosine Triphosphate; D011188:Potassium; D002118:Calcium; D002746:Chlorpromazine