title: Comparison of two supravital stains in examination of human semen and in tests for cytotoxic antibodies to human spermatozoa.
abstract: Supravital staining of human spermatozoa using trypan blue was compared with eosin Y staining. The dyes were applied for determination of the percentage of "dead" spermatozoa in semen samples and for determination of complement-dependent antisperm antibody activity in serum. Supravital staining percentages with trypan blue were significantly lower than with eosin Y.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D003601:Cytotoxicity Tests, Immunologic; D004801:Eosine Yellowish-(YS); D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology*; D013194:Staining and Labeling; D014343:Trypan Blue
chemical_list: D000906:Antibodies; D014343:Trypan Blue; D004801:Eosine Yellowish-(YS)