Effect of dextran and modified dextrans on the uptake and degradation of beta-galactosidase in isolated liver cells.
Conjugation of beta-galactosidase with either dextran, methylated dextran or acetylated dextran had only a small effect on uptake of the enzyme in isolated rat parenchymal and nonparenchymal liver cells. Conjugation of beta-galactosidase with dextran or the modified dextrans, reduced the intracellular degradation of the enzyme by up to about 45%. Methylated dextran had less effect than unmodified dextran or acetylated dextran on reducing the intracellular degradation of beta-galactosidase.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D003911:Dextrans / Q000494:pharmacology*; D005696:Galactosidases / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D013737:Testis / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism*
D003911:Dextrans; D007457:Iodine Radioisotopes; D005696:Galactosidases; D001616:beta-Galactosidase