Human T-cell leukemia virus in lymphocytes of two hemophiliacs with the acquired immunodeficiency syndrome.
Fresh and cultured peripheral blood cells from two patients with hemophilia A and the acquired immunodeficiency syndrome were examined for markers of infection with human T-cell leukemia virus (HTLV) type 1. Neither patient had antibody to membrane antigens of HTLV-infected cells at the time of culture. Electron microscopy of peripheral blood cells from Patient 1 and cultured cells from Patient 2 showed type C retrovirus-like particles. Examination of peripheral blood lymphocytes showed other smaller virus-like particles in circulating mononuclear cells from both patients. Indirect immunofluorescence of peripheral mononuclear cells from both patients and of cultured cells from Patient 2 showed staining with antibodies to purified HTLV and to HTLV core proteins p24 and p19.
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D000954:Antigens, Surface / Q000276:immunology; D002478:Cells, Cultured; D017977:Deltaretrovirus / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006467:Hemophilia A / Q000382:microbiology*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012192:Retroviridae Infections / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure
D000914:Antibodies, Viral; D000954:Antigens, Surface; D012194:RNA-Directed DNA Polymerase