Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.
A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.
D000818:Animals; D000937:Antigen-Antibody Reactions; D006046:Gold / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007158:Immunologic Techniques; D014066:Palatine Tonsil / Q000276:immunology*; D011817:Rabbits; D012834:Silver / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods
D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012834:Silver; D006046:Gold