[Tamoxifen fluorescence as a marker of hormone-dependence: physicochemical aspects].
In acid solutions, Tamoxifen is protonized and forms with eosin a fluorescent ionic association (lambda exc 480 nm, lambda em 565 nm). This reaction is quantitatively linked to the concentration of Tamoxifen. Thus the Tamoxifen induced fluorescence observed in hormone-dependent malignant breast tumor cells after Papanicolaou staining procedure, appears as a consequence of the binding of Tamoxifen to eosin.
D004801:Eosine Yellowish-(YS); D006801:Humans; D009376:Neoplasms, Hormone-Dependent / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013629:Tamoxifen
D013629:Tamoxifen; D004801:Eosine Yellowish-(YS)
La fluorescence du tamoxifène comme marqueur de l'hormonodépendance: aspect physico-chimique.