High-performance liquid chromatographic investigations on the time-dependent reaction of cis-Pt(NH3)2Cl2, Pt(en)Cl2 and Pt(pn)Cl2 with RNA fragments.
The reaction of three platinum compounds, Pt(NH3)2Cl2, Pt(en)Cl2 and Pt(pn)Cl2 with nucleic acid fragments (Ade, Gua; GpG, GpA and ApA), both reactants (1:1, 1 X 10(-2) M; 1 X 10(-3) M) being dissolved in water, has been studied. UV measurements have been used to characterize the products. Kinetics and adduct formation in the nucleoside-Pt compound system were studied by reversed-phase high-performance liquid chromatography [gradient 0-30% methanol in 30 min, ammonium acetate buffer (0.1 M) at pH 4.25] at fixed intervals of time and the product formation was examined by measuring the peak heights. After 10 h, the reaction was complete for guanosine and showed a steady state for adenosine. The increasing lipophilicity in the sequence of the platinum compounds resulted in a shift to longer retention times.
D000241:Adenosine / Q000031:analogs & derivatives / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015226:Dinucleoside Phosphates; D004347:Drug Interactions; D006150:Guanine Nucleotides / Q000032:analysis; D006151:Guanosine / Q000031:analogs & derivatives / Q000032:analysis; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009944:Organoplatinum Compounds / Q000032:analysis*; D012313:RNA / Q000032:analysis*
D015226:Dinucleoside Phosphates; D006150:Guanine Nucleotides; D009944:Organoplatinum Compounds; D006151:Guanosine; C002892:platinum ethylenediamine dichloride; C018309:guanylyl-(3'-5')-guanosine; C042975:platinum propylenediamine dichloride; D012313:RNA; C013011:guanine ribonucleotidyl-(3'-5')-adenosine; D006157:Guanosine Monophosphate; D000241:Adenosine