[Development of a competitive enzyme immunoassay for factor VIII-related antigen].
An enzyme immunoassay system basing on a competitive method has been developed to measure factor VIII related antigen (F. VIII R:Ag). A sufficient discrimination at low F. VIII R:Ag concentrations was gained. This method appears to be sensitive to 7,8 X 10(-3) U/ml F. VIII R:Ag showing an intraassay coefficient of variation (CV) of 0,11. In comparison to the commonly used Laurell electroimmunodiffusion assay for factor VIII significant less antisera per sample for the enzyme immunoassay technique is necessary.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis* / Q000276:immunology / Q000592:standards; D001667:Binding, Competitive; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005169:Factor VIII / Q000032:analysis / Q000276:immunology* / Q000592:standards; D006467:Hemophilia A / Q000097:blood / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques / Q000592:standards; D014842:von Willebrand Diseases / Q000097:blood / Q000276:immunology; D014841:von Willebrand Factor
D000941:Antigens; D007106:Immune Sera; D014841:von Willebrand Factor; D005169:Factor VIII
Entwicklung eines kompetitiven Enzymimmunoassay für das Faktor-VIII-assoziierte Antigen.