Mannose-sensitive and Gal-Gal binding Escherichia coli pili from recombinant strains. Chemical, functional, and serological properties.
Chromosomal genes encoding the MS and Gal-Gal binding properties have been cloned into separate recombinants and their respective pili characterized. Hapten inhibition of hemagglutination with synthetic carbohydrate receptor analogues and carbohydrate-adsorbed latex agglutination studies indicate that Gal-Gal and MS pili collectively exhibit the binding properties of the parent strain. MS pili migrated in SDS-PAGE with an Mr of 19 kdaltons and 17 kdaltons; the Mr of Gal-Gal pili was 17.5 kdaltons. The pili are chemically similar by amino acid composition and when the N-terminal cysteines are aligned, 8 of the 13 residues between positions 9 and 22 are homologous. Further, carboxy-terminal sequence homology was inferred from the carboxypeptidase digestion of a MS pili and the sequence of a carboxy-terminal tryptic peptide from Gal-Gal pili.
D000372:Agglutination Tests; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D004187:Disaccharides / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D008358:Mannose / Q000494:pharmacology*; D011995:Recombination, Genetic
D000596:Amino Acids; D001426:Bacterial Proteins; D004187:Disaccharides; D000939:Epitopes; D008358:Mannose; D005690:Galactose