title: Spot blot: a hybridization assay for specific DNA sequences in multiple samples.
abstract: Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively. After it is overlaid with a thin agarose cover, the DNA is denatured and transferred to nitrocellulose filters for hybridization. This method, called the "spot-blot assay," is particularly convenient when dealing with large numbers of samples.
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004926:Escherichia coli; D004996:Ethidium; D008722:Methods; D009693:Nucleic Acid Hybridization; D010804:Physarum / Q000235:genetics; D012313:RNA / Q000032:analysis
chemical_list: D012313:RNA; D004247:DNA; D004996:Ethidium