Lipid-bound sugars in malignant human breast tumors. Partial characterization of mannosyl and glucosyl transferase activities.
Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-[14C]-Man and UDP-[14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phosphate mannose and dolichyl phosphate glucose whereas lipid-linked oligosaccharides behaved as polyprenyl diphosphate derivatives. The optimum conditions for mannosyl- and glucosyl-transfer reactions and the effect of dolichyl phosphate, detergent and EDTA on incubation mixture were described.
D001943:Breast Neoplasms / Q000201:enzymology*; D002250:Carbon Radioisotopes; D005260:Female; D005964:Glucosyltransferases / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008364:Mannosyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000096:biosynthesis; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006023:Glycoproteins; D009702:Nucleoside Diphosphate Sugars; D011104:Polyisoprenyl Phosphate Oligosaccharides; D014539:Uridine Diphosphate Sugars; D006155:Guanosine Diphosphate Mannose; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D014532:Uridine Diphosphate Glucose