A monoclonal antibody to human transferrin receptor which inhibits erythroid differentiation of human leukemic K-562 cells.
In this paper we demonstrate that the 42/6 monoclonal antibody to human transferrin receptor (1) inhibits erythroid differentiation of human leukemic K-562 cells without affecting cell proliferation. Erythroid induction was monitored by benzidine-staining of K-562 cell suspensions and hemoglobin accumulation by cellulose acetate gel electrophoresis of post-mitochondrial cell lysates (4,5). Our results suggest that erythroid differentiation and cell growth require a different number of transferrin receptors.
D000818:Animals; D000911:Antibodies, Monoclonal; D001374:Azacitidine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation; D002460:Cell Line; D004588:Electrophoresis, Cellulose Acetate; D004900:Erythroblasts / Q000166:cytology*; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011956:Receptors, Cell Surface / Q000276:immunology*; D011990:Receptors, Transferrin
D000911:Antibodies, Monoclonal; D002087:Butyrates; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D020148:Butyric Acid; D006427:Hemin; D001374:Azacitidine