[Effect of methotrexate on cell growth and the production of hCG, beta-hCG and SP-1 in cultured choriocarcinoma cell lines].
Three choriocarcinoma cell lines (BeWo, GCH-2 and SCH) cultured in MTX or MTX-free medium were investigated with respect to cell growth and production of hCG, beta-hCG and specific beta-1-glycoprotein (SP-1). MTX was administered at the concentration of 10(-8), 10(-7), 10(-6), 10(-5)M for 24 hours, 72 hours and 168 hours from the fourth day of culture. Each cell line demonstrated that the cell growth and production of hCG and beta-hCG were dose, rather than time, dependent. 10(-6)M or more of MTX had a distinct effect, but 10(-7) or less of MTX had little effect. SP-1 was produced at a low level during culture regardless of the medium used. The ratio of the extra- to the intracellular hCG level was shown to be about 100:1 after the administration of MTX to BeWo and GCH-2. It is, therefore, possible that the temporary elevation of the hCG level observed in vitro after treatment with MTX is the result of an increase in secretion of hCG, rather than cellular damage.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000378:metabolism* / Q000473:pathology; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D006224:Cricetinae; D005260:Female; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D010446:Peptide Fragments / Q000096:biosynthesis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000096:biosynthesis*; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D008727:Methotrexate