Preparative separation of intact incomplete and empty adenovirus type 2 particles.
Complete adenovirus type 2 virions containing the whole genome were resistant to while empty capsids lacking DNA were breakable at a separation procedure involving customary CsCl gradient centrifugation. A purification method was developed using CsCl density gradient centrifugation in 5% glycerol which retained the integrity of incomplete particles as well as empty capsids. This procedure enabled to study the biological and physicochemical properties of different adenovirus type 2 particles.
D000260:Adenoviruses, Human / Q000302:isolation & purification* / Q000648:ultrastructure; D002213:Capsid / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D005990:Glycerol / Q000494:pharmacology; D009942:Organometallic Compounds; D013194:Staining and Labeling; D014501:Uranium; D014764:Viral Proteins / Q000302:isolation & purification*
D009942:Organometallic Compounds; D014764:Viral Proteins; C005460:uranyl acetate; D014501:Uranium; D005990:Glycerol