The passive transfer of severe allergic neuritis in Lewis rats with lymphoid cells preincubated with P2 protein.
The passive transfer of both clinical signs and histologic lesions characteristic of allergic neuritis was successfully performed in Lewis rats using pooled spleen and lymph node cells, or T lymphocytes therefrom, if first preincubated in petri dishes with P2 protein for 72 hr. For passive transfer, cells were taken from donors 8-16 days after sensitization with P2 protein or myelin in Freund's complete adjuvant, and administered via the tail vein; clinical signs appeared 12-13 days later. This study supports the importance of cell-mediated immunity in EAN and the antigenic role of the P2 protein.
D000818:Animals; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D004676:Myelin Basic Protein / Q000008:administration & dosage* / Q000276:immunology; D018996:Myelin P2 Protein; D009444:Neuritis, Autoimmune, Experimental / Q000209:etiology / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D004676:Myelin Basic Protein; D018996:Myelin P2 Protein