Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry.
The in situ localization of U1 and U2 snRNPs was examined, using autoantibodies directed against each of these snRNPs, by immunofluorescence and immunoelectron microscopy. This study has shown U1 and U2 snRNPs to colocalize in the nuclei of PtK2 cells. Thirty to fifty immunostained clusters were observed per interphase nucleus. This study suggests these nuclear protein clusters to be the sites of RNA processing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D007614:Macropodidae; D012313:RNA / Q000032:analysis* / Q000276:immunology; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins / Q000032:analysis* / Q000276:immunology; D017411:Ribonucleoproteins, Small Nuclear
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D012313:RNA