Electrophoretic elution of proteins from polyacrylamide gel slices.
A method for electrophoretic elution of proteins from polyacrylamide gel slices is described. Eluted proteins were retained by a discontinuous conductivity gradient (M. Otto and M. Snejdárková, Anal. Biochem. 111, 111-114 (1981)). The method has been adapted to slices from slab gels and gels that have been stained and destained. Proteins were eluted as their sodium dodecyl sulfate complexes. Minute amounts of proteins (0.1 microgram) were recovered in high yield (85-95%) in 2 h in less than 0.1 ml volume.
D001275:ATP Citrate (pro-S)-Lyase / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D001275:ATP Citrate (pro-S)-Lyase