Cytostatic effects of bleomycin and cis-platinum in A549 human lung tumour cells using autofeeder conditions.
Residual colony forming ability (CFA) of cells from a human alveolar carcinoma (A549) was determined after 48 hours of contact with bleomycin (BLM) or cisdichlorodiammineplatinum (II) (DDP) using feeder cells arising from parallel cultures left in contact with sublethal BLM or DDP concentrations. The CFA of untreated cells was 60 to 80% (45% with X-irradiated feeder cells). Growing cultures were more resistant to DDP and more sensitive to BLM than plateau phase cultures. Subclones arising from a first treatment were resistant to a second BLM or X-ray treatment, but more sensitive to a second DDP treatment.
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000188:drug therapy* / Q000532:radiotherapy; D001761:Bleomycin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects* / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002945:Cisplatin / Q000494:pharmacology*; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy
D001761:Bleomycin; D002945:Cisplatin