Binding of [125I]Bolton Hunter conjugated eledoisin to rat brain cortex membranes--evidence for two classes of tachykinin receptors in the mammalian central nervous system.
[125I]Bolton Hunter conjugated eledoisin was prepared and purified by ion-paired reverse phase chromatography. The ligand binds to rat brain cortex membranes, and the binding is inhibited over 95% by unlabeled eledoisin (6.6 microM). The binding site appears to be distinct from the [125I]Bolton Hunter conjugated substance P receptor based on the relative potencies of substance P, eledoisin, kassinin, physalaemin and [pGlu]substance P (6-11) hexapeptide to displace the binding of these two ligands.
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002540:Cerebral Cortex / Q000378:metabolism*; D004600:Eledoisin / Q000378:metabolism*; D015286:Kassinin; D009842:Oligopeptides / Q000378:metabolism; D010803:Physalaemin / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018039:Receptors, Tachykinin; D013373:Substance P / Q000378:metabolism; D013388:Succinimides
D009842:Oligopeptides; D011956:Receptors, Cell Surface; D018039:Receptors, Tachykinin; D013388:Succinimides; D010803:Physalaemin; D013373:Substance P; D015286:Kassinin; C022581:Bolton-Hunter reagent; D004600:Eledoisin