title: The effect of exercise and fasting on the myocardial protein and lipid metabolism in experimental bacterial myocarditis.
abstract: A generally nonlethal Salmonella typhimurium infection in weanling rats produced bacterial myocarditis and myocardial hyperplasia. Myocardial lesions were characterized by focal infiltrates of inflammatory cells (predominantly mononuclear), segmental myocyte necrosis, and incipient fibrosis. Although bacterial infections are infrequently associated with myocarditis, the S. typhimurium infection in young rats produced a new experimental model of diffuse myocardial inflammatory foci. Biochemical changes in the myocardium included great increases in total myocardial contents of protein (23%), RNA (39%) and DNA (43%) and several lipid fractions (35-55%) as well as in tissue activities of acid hydrolases, such as cathepsin D (124%) and beta-glucuronidase (135%), all of which contrasted with the relatively limited areas of histologic involvement (1.5%). To study the effects of additional stress in this model infection, some rats were exercised by forced running in wheels for 2 hours and others were fasted for 24 hours before samples were obtained. The short period of forced exercise in this infection caused an additional increase of myocardial protein content (47%) but with no additional change in histology. The expected fasting-induced degradation of protein as well as an infection-associated increase in myocardial lipids were each prevented when rats were fasted during ongoing acute infection. Protein degradation, as reflected by heightened acid hydrolase activities, seemed to occur at a similar rate regardless of other stresses, whereas the rate of myocardial protein synthesis appeared to be alterable.
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000032:analysis; D005215:Fasting; D005966:Glucuronidase / Q000032:analysis; D050356:Lipid Metabolism; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D009205:Myocarditis / Q000209:etiology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D005082:Physical Exertion; D012313:RNA / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012480:Salmonella Infections; D012486:Salmonella typhimurium
chemical_list: D009124:Muscle Proteins; D012313:RNA; D005966:Glucuronidase; D002402:Cathepsin D