[Pharmacological studies on oxatomide: (4) Effect on the histamine release from isolated rat peritoneal exudate cells (PEC) and lung slices].
The present study was carried out to evaluate the inhibitory effect of oxatomide and disodium cromoglycate (DSCG) on the histamine release from rat PEC and lung slices induced by antigen-antibody reaction, concanavalin A, compound 48/80 and A-23187. Oxatomide in the range of 1 microM to 10 microM inhibited the allergic histamine release from rat PEC and lung slices induced by antigen-antibody reaction in the presence of phosphatidyl-L-serine (PS). Although the inhibitory action of oxatomide was significant even at a concentration of 1 microM, the action did not increase with an increase in concentration of the drug. DSCG showed a concentration-dependent inhibition on the allergic histamine release from rat PEC and lung slices in the range of 10 microM to 100 microM. The histamine release from rat PEC induced by the combined treatment of concanavalin A and PS was inhibited by oxatomide (0.1 to 10 microM) and DSCG (10 to 100 microM). Oxatomide (up to 10 microM) showed no effect on the histamine release induced by compound 48/80, while DSCG in the range of 10 microM to 100 microM showed a concentration dependent inhibition of the release. Oxatomide at a concentration of 10 microM showed a significant inhibition on the histamine release induced by the calcium ionophore A-23187. On the other hand, DSCG had no effect on this reaction. Oxatomide at high concentrations did not stimulate the histamine release by itself.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D000001:Calcimycin / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D006634:Histamine H1 Antagonists; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D010879:Piperazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006634:Histamine H1 Antagonists; D010879:Piperazines; D003208:Concanavalin A; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; C015408:oxatomide; D004205:Cromolyn Sodium