Estimation of cell DNA synthesis rate from flow-cytometric histograms.
The rate of DNA synthesis along S-phase can be estimated on the basis of a simple model of a cell population, from a single FCM histogram under conditions of balanced exponential growth, and from two histograms suitably spaced in time under conditions of locally exponential S-phase influx. An algorithm that constructs a piecewise constant approximation of the synthesis rate and estimates the phase fractions from a single histogram is presented. The results of the application of the algorithm to a set of computer generated histograms in balanced exponential growth are reported.
D000818:Animals; D004261:DNA Replication; D005434:Flow Cytometry / Q000379:methods; D007399:Interphase; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological