Incomplete expression of the MOPC 460 idiotype in the sera of BALB/c mice immunized either with DNP antigen or with anti-idiotypic antibodies.
The MOPC 460 idiotype, as defined by polyclonal probes, has been described as a recurrent marker among the anti-DNP antibodies synthetized by IghCa mice. In this paper, we demonstrate, using syngeneic monoclonal anti-idiotypic probes, that only a part of the idiotopes of this idiotype are indeed recurrently expressed in BALB/c mice (IghCa) after immunization with DNP antigen. We will also show that the immunization of BALB/c mice with monoclonal anti-idiotypic antibody specific for the recurrent determinant results firstly in the synthesis of anti-DNP antibodies and secondly in the expression of the same recurrent M460 idiotope present on a part of induced anti-DNP molecules. Contrary to this, the immunization with the monoclonal anti-idiotypic antibody specific for the private idiotope never resulted in the synthesis of anti-DNP antibodies. These results clearly suggest that, after DNP or anti-idiotypic immunization, the M460 idiotype is not expressed in its entirety.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D004136:Dinitrobenzenes / Q000276:immunology*; D000939:Epitopes; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology*; D011863:Radioimmunoassay; D013997:Time Factors
D000906:Antibodies; D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins; D009578:Nitrobenzenes