title: A reappraisal of antigenic determinants for nitrogenase from Azotobacter and nitrate reductase from Escherichia coli.
abstract: Previous work based on double immunodiffusion assays had shown that there are common antigenic determinants for nitrate reductase from Escherichia coli and component I of nitrogenase from Azotobacter vinelandii. Further work reported herein using a variety of immunoelectrophoretic techniques indicates that the cross-reaction between nitrate reductase and antiserum to component I of nitrogenase results from a contaminant antigen co-purified with nitrate reductase.
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology*; D001395:Azotobacter / Q000201:enzymology*; D003429:Cross Reactions; D000939:Epitopes; D004926:Escherichia coli / Q000201:enzymology*; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D009565:Nitrate Reductases / Q000276:immunology*; D009591:Nitrogenase / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D009591:Nitrogenase; D009565:Nitrate Reductases