title: Application of the microcomputer in multiparametric fluorescence cytophotometry.
abstract: Methods for combined cytofluorometric determinations of nuclear DNA plus other parameters (proteins, enzyme activity, or immunoreactive substances) are reported. DNA measurement was performed on an autoradiograph of Feulgen-stained fetal mouse hepatocytes. In order to facilitate the multiparametric measurements and data processing, a fluorescence cytophotometer (BH2-QRFL, Olympus, Tokyo) equipped with four interchangeable filter-sets and controlled by a desktop computer (HP-85F), was used. All data stored in the same computer were displayed in a two-dimensional scatter plot or two-dimensional frequency distribution histogram. Analysis of the DNA histogram of fetal mouse hepatocytes was accomplished by the same computer. Calculated fraction sizes for G, S, and G plus M phases were found to be in good correlation with those determined directly on the simultaneously combined tritiated thymidine (3H-TdR) autoradiography.
mesh_terms: D000328:Adult; D000818:Animals; D000941:Antigens; D001345:Autoradiography; D003201:Computers; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D051379:Mice; D008838:Microcomputers; D008875:Middle Aged; D010544:Peroxidases / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D013194:Staining and Labeling
chemical_list: D000941:Antigens; D011506:Proteins; D004247:DNA; D010544:Peroxidases