Purification of poly(A)-messenger ribonucleic acid by reversed-phase high-performance liquid chromatography.
Polyadenylated messenger ribonucleic acid [poly(A)-mRNA] was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Partially purified poly(A)-mRNA was prepared from rat liver polysomes by magnesium precipitation followed by affinity chromatography on an oligo(dT) cellulose column. Translatable poly(A)-mRNA, as assayed by in vitro translation in a rabbit reticulocyte system, was resolved from non-translatable RNA by RP-HPLC. The separation appears to be due to the presence of poly(A) residues on the 3' terminus of the mRNA.
D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005914:Globins / Q000032:analysis; D008099:Liver / Q000032:analysis; D011061:Poly A / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger; D011817:Rabbits; D051381:Rats; D012270:Ribosomes / Q000032:analysis
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D005914:Globins