Does Q beta replicase synthesize RNA in the absence of template?
Q beta replicase, in the absence of added template, will synthesize RNA autocatalytically. A variety of small RNa species, termed '6S RNAs' are generated. As this reaction purportedly occurs in the absence of template, it has been termed 'de novo' RNA synthesis. The question of whether Q beta replicase can polymerize replicatable RNA molecules, without instruction from a template, has important evolutionary implications. The finding that Q beta replicase was able to synthesize RNA de novo was based on (1) failure to find contaminating RNA in Q beta replicase preparations; (2) differences in the sizes of products of apparently identical reactions; and (3) kinetic differences between template-instructed and de novo reactions. Here wer describe a procedure for production of Q beta replicase lacking one of its subunits, ribosomal protein S1, involving column chromatography in the presence of a low concentration of urea. We show that the resulting highly purified enzyme will not synthesize detectable RNA in the absence of added template. We show also that the ability to perform a reaction kinetically indistinguishable from the de novo synthesis reaction can be restored to the highly purified enzyme by adding a heat-stable, alkali-labile component of Q beta replicase preparations. Thus our findings suggest that, in the novo reaction, Q beta replicase is replicating previously undetected contaminating RNA molecules.
D002848:Chromatography, DEAE-Cellulose; D011108:Polymers; D011777:Q beta Replicase / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000302:isolation & purification; D013698:Templates, Genetic; D014508:Urea
D011108:Polymers; D012269:Ribosomal Proteins; D012313:RNA; D014508:Urea; D011777:Q beta Replicase; D012316:RNA Nucleotidyltransferases