[Immunohematological study of 7 cases of acquired B transformation using anti-B and selected acquired anti-B].
Seven acquired B samples were studied with selected anti-B sera. Eight sera came from group A subjects, four from group O subjects. The anti-B sera reacting with the acquired B antigen are always a mixture of two kinds of antibodies: a fraction that is only reactive with B antigen, another that is cross-reactive with B and acquired B antigens. In some sera, especially those coming from group O subjects, there is, in addition, a potent antibody of polyagglutination for acquired B red cells. The polyagglutinability associated with acquired B red cells is analysed. According to the results obtained using lectins, four red cell samples are merely acquired B, the three others are acquired B + Tk. The polyagglutinability associated with Tk transformation is non sensitive to acetylation of red cells. The polyagglutinability specific for acquired B is sensitive to acetylation but appears complex in itself. According to the reactivity of selected sera for acquired B samples and the studies of inhibition with simple sugars, there are at least two antigens involved. One of them is present in all of the acquired B samples and it is likely to have arisen from the action of a deacetylase on a structure including N-acetylgalactosamine.
D000017:ABO Blood-Group System / Q000276:immunology*; D000107:Acetylation; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D005700:Galactosyltransferases / Q000097:blood* / Q000302:isolation & purification; D006386:Hemagglutination Tests; D006801:Humans; D007518:Isoantibodies / Q000276:immunology / Q000502:physiology; D037102:Lectins / Q000494:pharmacology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007518:Isoantibodies; D037102:Lectins; D005700:Galactosyltransferases; C023155:blood-group-substance alpha-D-galactosyltransferase
Etude immunohématologique de 7 transformations B acquis à l'aide d'anti-B et d'anti-B acquis sélectionnés.