Fine specificity of antibodies to the synthetic polypeptide poly(L-tyrosine, L-glutamic acid)-poly(DL-alanine)--poly(L-lysine) and its ordered analogs as followed by solid-phase radioimmunoassay.
The fine specificity of antibodies against (T,G)-A--L and its ordered analogs (T-T-G-G)-A--L and (T-G-T-G)-A--L was studied. Fifty percent of the antibodies against (T,G)-A--L are directed toward the T-T-G-G determinants and 19% against T-G-T-G-like determinants. The rest of the antibody response to (T,G)-A--L is directed against determinants which exist in (T,G)-A--L but are not cross-reactive with either T-T-G-G- or T-G-T-G-like determinants. Although (T-T-G-G)-A--L and (T-G-T-G)-A--L differ only in the sequence of tyrosine and glutamic acid in their side chains, no crossreactivity was observed between antibodies toward the two ordered polypeptide antigens.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D007106:Immune Sera; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides; C021759:(T,G)-A-L