The metabolism of 8-methoxypsoralen by Saccharomyces cerevisiae. Evidence for an inducing effect of ethanol.
The metabolism of 8-methoxypsoralen (8-MOP) by the yeast Saccharomyces cerevisiae has been investigated in order to determine if this cell system could provide a simple model to study the metabolism of new photosensitizing drugs in vitro. 8-MOP was found to be rapidly metabolized by S. cerevisiae in non growing conditions. A metabolite was detected which exhibits a structure similar to that of a metabolite previously isolated in dog and man. Ethanol showed a strong inducing effect on 8-MOP metabolization.
D001711:Biotransformation; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D008730:Methoxsalen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013997:Time Factors; D014466:Ultraviolet Rays
D003470:Culture Media; D000431:Ethanol; D008730:Methoxsalen