Cytokeratin filament expression during in vitro teratocarcinoma cell differentiation as detected by a monoclonal antibody.
Undifferentiated F9 teratocarcinoma cells were induced to differentiate in culture using retinoic acid and cAMP. As a result the morphology of the cultures changes dramatically. Using a monoclonal antibody directed against cytokeratin polypeptide 18 (RGF 53) in the indirect immunofluorescence technique we could show that this cytokeratin subunit is synthesized and assembled into a filamentous network upon differentiation in about 50% of the cells. Immunoblotting studies confirm these results.
D000818:Animals; D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000503:physiopathology; D002454:Cell Differentiation; D002460:Cell Line; D003599:Cytoskeleton / Q000502:physiology*; D006801:Humans; D007633:Keratins / Q000032:analysis*; D008113:Liver Neoplasms / Q000503:physiopathology; D051379:Mice; D008858:Microscopy, Phase-Contrast; D013724:Teratoma / Q000503:physiopathology*
D000911:Antibodies, Monoclonal; D007633:Keratins