DNA sequence polymorphism in an androgen-regulated gene is associated with alteration in the encoded RNAs.
We have used plasmid pMK908, whose cDNA insert corresponds to an androgen-inducible RNA from mouse kidney, as a probe to study both the complementary genomic DNA and the encoded RNA sequences in several inbred strains of mice. A polymorphism in the 908 structural gene, revealed by Southern blotting of HindIII-generated DNA fragments, was found to map near the Gpi-1 and Tam-1 loci on chromosome 7. The 908 structural gene has been termed RP2. The 908 RNAs constitute a sequence-related group displaying extensive size heterogeneity. This heterogeneity, reflected in the size distribution of the RNA on electrophoretic blots, is controlled by a genetic site that is tightly linked to RP2. Thus, associated with polymorphism at RP2 is a change in the molecular size pattern of encoded transcripts.
D000483:Alleles; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes / Q000187:drug effects*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000187:drug effects*; D011110:Polymorphism, Genetic; D012313:RNA / Q000235:genetics; D013739:Testosterone / Q000494:pharmacology*; D014158:Transcription, Genetic
D013739:Testosterone; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes