A new procedure for coupling antibody to paper discs for radioimmunoassay: application to the determination of alpha-fetoprotein.
Horse anti-alpha-fetoprotein was coupled to CM-cellulose discs by a modified carbodiimide reaction. The resulting coupling CM-discs were used in solid-phase radioimmunoassay of human alpha-fetoprotein. The sensitivity of these discs and conventional BrCN activated filter paper discs coupled anti-alpha-fetoprotein was approximately the same. A fair correlation between the alpha-fetoprotein levels determined by both methods was observed. The coupling procedure with carbodiimide is simple and the use of hazardous BrCN is eliminated.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D005260:Female; D006736:Horses; D006801:Humans; D007202:Indicators and Reagents; D010209:Paper; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*; D000509:alpha-Fetoproteins / Q000032:analysis*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins