A fast silver staining method for protein detection after isoelectric focusing in agarose gels.
A sensitive staining method was developed for detecting proteins in agarose gels after isoelectric focusing. Its sensitivity is about 20 times that of the Coomassie blue R-250 staining technique, and the time required is only 10 min.
D007525:Isoelectric Focusing; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling; D001613:beta 2-Microglobulin / Q000302:isolation & purification*
D012394:Rosaniline Dyes; D001613:beta 2-Microglobulin; D012834:Silver; C004692:coomassie Brilliant Blue