Clonal analysis of T-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper T-cell clone.
A herpes simplex virus (HSV)-specific long-term T-cell clone has been established from the draining lymph node cells of BALB/c mice; the cells required repeated in vitro restimulation with UV-irradiated virus. The established T-cell clone expresses the Thy-1 and Lyt-1+2,3- surface antigens. For optimal proliferation of the cloned cells, both the presence of specific antigen and an exogenous source of T-cell growth factor are required. The proliferative response of the cloned T cells was found to be virus-specific but it did not distinguish between HSV-1 and HSV-2. Adoptive cell transfer of the cloned T cells helped primed B cells to produce anti-herpes antibodies: the response was antigen-specific and cell dose-dependent. The clone failed to produce a significant DTH reaction in vivo, but did produce high levels of macrophage-activating factor. Furthermore, the T-cell clone could protect from HSV infection, as measured by a reduction in local virus growth, and by enhanced survival following the challenge of mice with a lethal dose of virus. The mechanism(s) whereby this clone protects in vivo is discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000954:Antigens, Surface / Q000032:analysis; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006561:Herpes Simplex / Q000276:immunology; D006968:Hypersensitivity, Delayed; D007116:Immunization, Passive; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000096:biosynthesis; D016215:Macrophage-Activating Factors; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D018139:Simplexvirus / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000939:Epitopes; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors