Immunochemical analysis of substance P using its synthetic fragments and analogs.
Synthetic fragments and analogs were used to characterize specificity of antisera to substance P. Both, the C-terminal hexapeptide and the pentapeptide completely inhibited binding of 125I-[Tyr8]substance P by these antisera, showing the antigenic identity with substance P. Synthetic fragments shorter than peptide (7-11) did not react with anti-substance P antisera in this system. Substitution of amino acids in different positions in the fragments (6-11) or (7-11) by histidine or glycine revealed that all five amino-acid residues take part in a structure of the antigenic determinant.
D000818:Animals; D002417:Cattle; D007120:Immunochemistry; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013373:Substance P / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology
D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; D013373:Substance P