Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining.
This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obtain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 column. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D019393:Arrestin; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D012160:Retina / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D000941:Antigens; D019393:Arrestin; D012834:Silver