High-performance liquid chromatographic determination of indoleamines, dopamine, and norepinephrine in rat brain with fluorometric detection.
A high-performance liquid chromatography-fluorescence procedure for the determination of 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, tryptophan, dopamine, and norepinephrine has been developed. The method uses an ion-pairing system on an Ultrasphere ODS (5-microns) column with detector wavelength settings of excitation at 290 nm and emission at 330 nm. The procedure has been used to quantitate these indoleamines and catecholamines in rat brain tissue after homogenization in a perchloric acid solution; an aliquot of this solution is injected directly onto the HPLC column. Column sensitivities range from 6.1 pmol for tryptophan to 1.1 pmol for 5-hydroxytryptamine.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis*; D005470:Fluorometry; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D007211:Indoles / Q000032:analysis*; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000032:analysis; D014364:Tryptophan / Q000032:analysis
D007211:Indoles; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D004298:Dopamine; D009638:Norepinephrine