Idiotypes of anti-phosphocholine antibodies: structural correlates.
Phosphocholine-specific antibodies in mice are composed of three families of antibodies, the T15, the M603 and the M511, which are constructed by combinatorial association of a VH4 H chain and one of three different L chains, VK22, VK8 and VK24, respectively. Antiidiotypic antisera can be generated which (1) recognize all members of a family, i. e. anti-T15 IdX, anti-M603 IdX and anti-M511 IdX, (2) distinguish between members of a family, e. g. A/J anti-T15, or (3) recognize determinants on only a single anti-PC antibody. Consideration of protein sequences and L-chain polymorphisms and analysis of chain recombination experiments among Id-positive and -negative antibodies revealed that IdX are determined by the L chain. A similar approach demonstrated that the Id determinant defined by the A/J anti-T15 is dependent upon the L chain and the D region of the H chain.
D000595:Amino Acid Sequence; D000818:Animals; D002794:Choline / Q000031:analogs & derivatives*; D000939:Epitopes / Q000032:analysis*; D006168:Guinea Pigs; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D010767:Phosphorylcholine / Q000276:immunology*; D011817:Rabbits
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D002794:Choline