DNA cloning of Plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope.
A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS beta-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D000939:Epitopes / Q000235:genetics*; D005796:Genes; D008288:Malaria / Q000276:immunology; D010963:Plasmodium falciparum / Q000235:genetics*; D015800:Protozoan Proteins; D012091:Repetitive Sequences, Nucleic Acid
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan; D004247:DNA