Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.
Qualitative and quantitative tests were performed to determine whether the temperature at which dehydration and embedding occur affects the antigenic specificity of tubulin and the protein A-gold (pAg) immunolabeling technique. The analysis indicates that low temperature (-35 degrees C) treatment increased the specificity and density of pAg labeled anti-tubulin antibodies to Leishmania tropica subpellicular microtubules as compared to samples prepared at 0 degrees C or 20 degrees C.
D000180:Acrylic Resins; D003102:Colloids; D000939:Epitopes / Q000032:analysis*; D004853:Epoxy Resins; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D006652:Histological Techniques; D007158:Immunologic Techniques; D007891:Leishmania / Q000648:ultrastructure; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D013205:Staphylococcal Protein A; D013696:Temperature; D014404:Tubulin / Q000032:analysis* / Q000276:immunology
D000180:Acrylic Resins; D003102:Colloids; D000939:Epitopes; D004853:Epoxy Resins; D013205:Staphylococcal Protein A; D014404:Tubulin; C004875:EPON; D006046:Gold; C037883:Lowicryl 4KM resin