IgE-mediated 14C-serotonin release from passively sensitized rat mast cells: comparative kinetic study with formaldehyde and ice-cold methods.
The kinetics of IgE-mediated release of serotonin from passively sensitized rat mast cells in vitro was studied by stopping 14C-serotonin release with the application of formaldehyde fixative or ice-cold mast cell medium (MCM). Antigen dose-release curves of 14C-serotonin and/or histamine were comparable when mediator release was terminated with either formaldehyde at a final concentration of 1% or ice-cold MCM 15 min after antigen challenge. However, the kinetic study of immunological mediator release stopped by formaldehyde showed that the addition of antigen resulted in a progressive increase of released 14C-serotonin for 7 min, the release curve being sigmoidal, whereas the application of ice-cold MCM artificially enhanced 14C-serotonin and histamine release in the first 2 min. The results suggest that stopping IgE-mediated release of 14C-serotonin with formaldehyde is a simple, rapid and accurate method of studying the kinetics of mediator release from mast cells.
D000818:Animals; D000941:Antigens / Q000276:immunology; D002250:Carbon Radioisotopes; D003080:Cold Temperature; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology*; D006636:Histamine Release; D007053:Ice; D007073:Immunoglobulin E / Q000276:immunology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D000941:Antigens; D002250:Carbon Radioisotopes; D007053:Ice; D005557:Formaldehyde; D012701:Serotonin; D007073:Immunoglobulin E