A transient species of poly(A)+ RNA detected by a myosin heavy chain cDNA probe in muscle cell culture during terminal differentiation.
Primary cell cultures were prepared from breast muscles of 11 day 4 hour-embryonic chicks. Cytoplasmic RNAs were isolated from the cultured cells at various time intervals from day 3 to day 8. A [P32] DNA probe complementary to messenger RNA of myosin heavy chain was used to hybridize with the RNAs after gel electrophoresis. A transient species of polyadenylated RNA with a decreased mobility in electrophoresis was detected during a period of time when contractions of syncytial fibers were first observed.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA; D006801:Humans; D009119:Muscle Contraction; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D009218:Myosins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D009218:Myosins