Effects of heparin and dextran sulphate on the production of collagen and protein in diabetic and non-diabetic human skin fibroblast cultures.
Addition of heparin and dextran sulphate to human skin fibroblasts in cell cultures caused an increase in [3H]-proline incorporation into collagen and total protein in the culture medium by cells derived from nondiabetics. Cells from type 2 diabetic subjects were significantly less affected by dextran sulphate addition, suggesting altered regulatory mechanisms for collagen production in these cells. Addition of chondroitin sulphate caused a dose-dependent increase in labelled collagen, indicating a possible role for this glycosaminoglycan as modulator of collagen deposition.
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis*; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D014176:Protein Biosynthesis; D012867:Skin / Q000378:metabolism
D003911:Dextrans; D007328:Insulin; D006493:Heparin; D002809:Chondroitin Sulfates; D003094:Collagen; D016264:Dextran Sulfate