title: The use of primuline to identify the septum polysaccharide of the fission yeast Schizosaccharomyces pombe.
abstract: Treatment of cells and purified cell walls of the fission yeast Schizosaccharomyces pombe with primuline reveals the septum as a bright fluorescent band. When polysaccharides containing (1----3)-beta-, (1----6)-beta- or (1----3)-alpha-glucosidic linkages are treated with primuline, only those molecules containing chains of (1----3)-beta-glucosyl residues are stained. This implies that (1----3)-beta-glucan is present in the septum of Schiz. pombe as the main constituent.
mesh_terms: D001203:Ascomycota / Q000032:analysis*; D002455:Cell Division; D002473:Cell Wall / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D012568:Schizosaccharomyces / Q000032:analysis* / Q000166:cytology; D013194:Staining and Labeling; D013844:Thiazoles
chemical_list: D011134:Polysaccharides; D013844:Thiazoles; C035646:primuline