Comparison of growth rates of two clones of Bacillus subtilis, producer of alpha-amylase.
In a population of the productive Bacillus subtilis strain the production of alpha-amylase differentiates at the cell level. Individual cells in the population were analyzed and the production of isolates was tested. The mean specific activity of alpha-amylase in the productive group after the isolation was 5.0 U/mg dry substance and in the nonproductive group 1.5 U/mg dry substance. This ratio remained unchanged during long-term observations and after repeated transfers. In selected strains of both groups the specific growth rate was determined in a synthetic medium containing various amounts of casein hydrolyzate. The detected differences in the growth rate between the productive and the nonproductive clones are determined by amino acid concentration.
D000681:Amylases / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D002999:Clone Cells; D013997:Time Factors; D000516:alpha-Amylases / Q000096:biosynthesis*
D000681:Amylases; D000516:alpha-Amylases