Inhibitors binding to L-aromatic amino acid decarboxylase.
The effect of a number of inhibitors of L-aromatic amino acid decarboxylase activity on the absorption spectrum of the enzyme-bound coenzyme has been studied. It has been observed that the compounds tested, even if devoid of the amino function and therefore unable to form the Schiff base with the coenzyme, modify significantly the enzyme spectrum, indicating their binding to the coenzyme active site. Spectral modifications suggest that at least two kinds of binding of inhibitors to L-aromatic amino acid decarboxylase may occur, depending on their structural features. Moreover, from the spectra obtained at different concentrations of the inhibitors their affinity constants have been determined: data indicate that the cathecol ring gives the largest contribution to the binding, while the presence of the carboxyl group, the aminic group and the aliphatic chain are responsible for a decrease in the binding, which could be relevant for the efficiency of the catalysis.
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D001665:Binding Sites; D002109:Caffeic Acids / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007980:Levodopa / Q000031:analogs & derivatives / Q000378:metabolism; D010648:Phenylacetates / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000378:metabolism; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002109:Caffeic Acids; D002396:Catechols; D010648:Phenylacetates; D007980:Levodopa; D006897:Hydroxyindoleacetic Acid; D011732:Pyridoxal Phosphate; D001142:Aromatic-L-Amino-Acid Decarboxylases; C035420:levodopa methyl ester