[Establishment of a DNA bank of human origin].
High molecular weight DNA extracted from 50 unrelated parisian individuals constitutes a "DNA-thèque" which can be used at the population level. This procedure allows preparation from a variety of tissues such as liver, placenta and leucocytes. The "quality" of the DNA obtained (about 50 Kb, RNA and protein free) and the efficiency of the method (in terms of the yield of DNA obtained) are considered. Blood is the easiest material to obtain and permits the obtaining of a reasonable amount of human and primate DNA, particularly when familial investigation is planned for polymorphism studies. One of the objects of this article is to encourage others to make use of our bank, for studies in population and evolutionary genetics.
D000818:Animals; D002144:Callithrix; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005828:Genetics, Population; D006801:Humans; D007901:Lemuridae; D007962:Leukocytes / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D002679:Pan troglodytes; D010215:Papio; D010375:Pedigree; D010920:Placenta / Q000032:analysis; D011506:Proteins / Q000032:analysis / Q000235:genetics; D012313:RNA / Q000032:analysis / Q000235:genetics; D014015:Tissue Banks; D014021:Tissue Preservation
D011506:Proteins; D012313:RNA; D004247:DNA
Constitution d'une banque d'ADN d'origine humaine.