Effect of the calcium ionophore A23187 and aspirin on histamine release in vitro from leukocytes of aspirin-intolerant donors.
In vitro histamine release from leukocytes of 10 aspirin-intolerant donors in response to the calcium ionophore A23187 and aspirin was compared to that of controls. Incubation with A23187 induced a concentration-dependent histamine release from leukocytes of all donors. At 0.1 and 0.2 micrograms/ml A23187, release from leukocytes of aspirin-intolerant donors was significantly less (p less than 0.01) than that of controls. Preincubation with aspirin failed to alter spontaneous or A23187-stimulated histamine release. Leukocytes of aspirin-intolerant donors do not demonstrate an enhanced sensitivity to histamine release stimulated by aspirin or A23187.
D000328:Adult; D000368:Aged; D001241:Aspirin / Q000276:immunology* / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004342:Drug Hypersensitivity / Q000276:immunology*; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged
D000001:Calcimycin; D001241:Aspirin