Determination of neurotransmitter systems in human cerebrospinal fluid and rat nervous tissue by high-performance liquid chromatography with on-line data evaluation.
We describe an improved high-performance liquid chromatographic method for the determination of tyrosine, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, tryptophan and homovanillic acid in cerebrospinal fluid and nerve tissue, using the new microbore cartridges with 5 micron average particle size. The first four substances are quantified fluorometrically and the last two electrochemically. Both detectors are connected to the same integrator through a relay which can be switched as required. Data are collected in an on-line personal computer and evaluated statistically. An improvement in the method for extraction and separation of catecholamines is also reported.
D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis / Q000134:cerebrospinal fluid; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006719:Homovanillic Acid / Q000032:analysis / Q000134:cerebrospinal fluid; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis / Q000134:cerebrospinal fluid; D008838:Microcomputers; D009417:Nerve Tissue / Q000032:analysis*; D018377:Neurotransmitter Agents / Q000032:analysis / Q000134:cerebrospinal fluid*; D051381:Rats; D012701:Serotonin / Q000032:analysis / Q000134:cerebrospinal fluid; D013050:Spectrometry, Fluorescence; D013116:Spinal Cord / Q000032:analysis; D014364:Tryptophan / Q000032:analysis / Q000134:cerebrospinal fluid; D014443:Tyrosine / Q000032:analysis / Q000134:cerebrospinal fluid
D002395:Catecholamines; D018377:Neurotransmitter Agents; D012701:Serotonin; D014443:Tyrosine; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D006719:Homovanillic Acid