The proline-, glycine-, glutamic acid-rich pink-violet staining proteins in human parotid saliva are phosphoproteins.
The major proteins in human parotid saliva, isolated in Fractions II-V following chromatography on Sephacryl S-200, DEAE-Sephadex A-50, or CM cellulose, contain 6 moles of phosphate per mole of protein, the phosphate probably bound to the protein via an ester linkage. This phosphate represents greater than 90% of the protein-bound phosphate in human parotid saliva. Neither purified gustin nor amylase, the two other major proteins in human parotid saliva, contain phosphate.
D055598:Chemical Phenomena; D002621:Chemistry; D005971:Glutamates / Q000032:analysis; D005998:Glycine / Q000032:analysis; D006801:Humans; D010306:Parotid Gland / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis*; D011392:Proline / Q000032:analysis; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013194:Staining and Labeling
D005971:Glutamates; D010750:Phosphoproteins; D012471:Salivary Proteins and Peptides; D011392:Proline; D005998:Glycine