Cross-reaction of snRNA and an Alu I-like sequence from rat with DNAs from different eucaryotic species.
Sequence homologies to rat U1-snRNA and U2-snRNA were investigated in DNA from 23 eucaryotic species (3 lower eucaryotes, 4 plants, and 16 animals) using dot hybridization at various stringency conditions. Cross-hybridization among very distantly related species in e.g. plants-insects or mold-vertebrates is not the rule; there are, however, examples for stronger homologies like Rattus-Dictyostelium. Furthermore, DNA from all 23 species was analysed for sequence homologies with the repetitive DNA sequence B1 (an Alu I family equivalent) from rat. We observed a wide range of homologies covering some plants and insects, up to vertebrates. Hybridization at increasing stringency conditions revealed species with higher degree of homology to the rat B1 sequence: maize, chicken, and hamster.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005658:Fungi / Q000235:genetics; D006801:Humans; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010944:Plants / Q000235:genetics; D011247:Pregnancy; D012313:RNA / Q000235:genetics*; D012342:RNA, Small Nuclear; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D012342:RNA, Small Nuclear; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific