Interaction of polymerases with 2'-deoxyuridine-5'-triphosphate spin-labeled at the 5-position.
2'-Deoxyuridine-5'-triphosphate spin-labeled at the 5-position with N-[1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl]-O- was found to be an inhibitor of some DNA and RNA polymerases including avian myeloblastosis virus reverse transcriptase. Furthermore, the spin-labeled nucleotide was found to be incorporated internally into polydeoxythymidylic acid via reverse transcriptase to an extent of 1.0 spin-labeled base per 10(3) bases. The incorporation, monitored by electron spin resonance, is analogous to some other nucleotide inhibitors of polymerases, and the results indicate that it may be feasible to obtain sequence specific, spin-labeled DNA, enzymatically.
D000818:Animals; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D009975:Orthomyxoviridae / Q000201:enzymology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013113:Spin Labels
D003856:Deoxyuracil Nucleotides; D013113:Spin Labels; C027078:deoxyuridine triphosphate; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase