Transformation of human lung cells by 3-methylcholanthrene in vitro.
Human fetal lung tissue was cultured on dead, sterile pigskin as a substrate and treated with 3-methylcholanthrene. Several cell lines were obtained. One of the lines produced a tumor in nude mouse and showed anchorage-independent growth in soft agar. The tumor cells were stained positive for keratin, indicating their epithelial origin.
D000912:Antibodies, Neoplasm / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D006801:Humans; D007633:Keratins / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D008748:Methylcholanthrene / Q000633:toxicity*
D000912:Antibodies, Neoplasm; D008748:Methylcholanthrene; D007633:Keratins