The distribution of acidic coomassie blue-stained proteins from uninvolved human liver, hepatoma, normal colon, primary colon cancer, and colon metastases to the liver, determined by two-dimensional protein electrophoresis.
The distribution of Coomassie blue-stained proteins from uninvolved regions of 4 human livers, from 1 hepatocellular carcinoma, and from 4 samples each of uninvolved colon, primary adenocarcinoma of the colon, and colon cancer metastatic to the liver was analyzed by two-dimensional protein electrophoresis. From a comparison of acidic proteins between pI 3.5 and 6.5, we conclude (1) that the majority (66 of 82) of denoted acidic proteins from 4 normal liver samples were represented in the hepatocellular carcinoma. Fifty-one of 58 proteins denoted in the 4 colon samples were detected in each of the 4 primary colon cancers; (2) that the "normograms" of proteins from normal colon and normal liver differed in many details, and their dissimilar patterns identified the source of the sample; (3) that hepatoma and primary adenocarcinoma of the colon were easily distinguished by their distribution of proteins; (4) that colon cancer metastatic to the liver contained a majority (50/58) of acidic proteins enumerated in primary colon cancer. These results indicate that uninvolved liver and colon and their primary or secondary cancers can be identified by their distribution of electrophoresed acidic proteins.
D000230:Adenocarcinoma / Q000032:analysis*; D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D003106:Colon / Q000032:analysis; D003110:Colonic Neoplasms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D008113:Liver Neoplasms / Q000032:analysis* / Q000556:secondary; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D012394:Rosaniline Dyes; D000509:alpha-Fetoproteins / Q000032:analysis
D007202:Indicators and Reagents; D009363:Neoplasm Proteins; D011506:Proteins; D012394:Rosaniline Dyes; D000509:alpha-Fetoproteins; C004692:coomassie Brilliant Blue