GDPmannose dehydrogenase and biosynthesis of alginate-like polysaccharide in a mucoid strain of Pseudomonas aeruginosa.
GDPmannose dehydrogenase (EC 1.1.1.132) in a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis was identified by demonstrating the NAD-linked formation of GDPmannuronate from GDPmannose mediated by a cell extract of the organism. Nonmucoid mutant strains did not contain GDPmannose dehydrogenase, which suggests that the enzyme is involved in the biosynthesis of alginate-like polysaccharide by P. aeruginosa.
D000464:Alginates / Q000096:biosynthesis*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000254:growth & development
D000464:Alginates; D009702:Nucleoside Diphosphate Sugars; D011135:Polysaccharides, Bacterial; C037228:GDP mannuronic acid; D006155:Guanosine Diphosphate Mannose; D002237:Carbohydrate Dehydrogenases; C020562:GDPmannose dehydrogenase