[Electron microscopy and cytochemistry studies of lymphocyte subpopulations in corticosteroid deficiency].
The authors studied the number, structure and function of different blood T lymphocyte subpopulations in experimental hypocorticoidism of male CBA mice with the use of modern hematological, cytochemical, immunological and electron microscopy methods. One week after bilateral adrenalectomy the blood showed a considerable absolute and relative lymphocytosis determined by an increase in the content of Thy I-positive, ANAE-positive (giving positive reaction during identification of acid non-specific alpha-naphthylacetate esterase) cells and large granule-containing lymphocytes, a group, which includes natural killers and cells possessing antibody-dependent cell-mediated cytotoxicity. Transmission and scanning electron microscopy demonstrated changes in submicroscopic structure of different blood lymphocytes in adrenalectomized mice. It may be inferred that during hypocorticoidism, there takes place a complicated immunological transformation resulting in the changes in the function of different T lymphocyte subpopulations and natural killers responsible for immune defence of the body.
D000315:Adrenalectomy / Q000009:adverse effects*; D000818:Animals; D003345:Corticosterone / Q000172:deficiency*; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000097:blood; D007694:Killer Cells, Natural / Q000648:ultrastructure*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D009283:Naphthol AS D Esterase / Q000097:blood; D012313:RNA / Q000097:blood; D013602:T-Lymphocytes, Cytotoxic / Q000648:ultrastructure*
D012313:RNA; D004247:DNA; D009283:Naphthol AS D Esterase; D003345:Corticosterone
Elektronno-mikroskopicheskoe i tsitokhimicheskoe issledovanie subpopuliastsii limfotsitov krovi pri nedostatke kortikosteroidov.