Retrograde transport of dopamine beta-hydroxylase antibodies in sympathetic neurons: effects of drugs modifying noradrenergic transmission.
Antibodies to dopamine beta-hydroxylase (anti-D beta H) were taken up by noradrenergic nerve terminals in the iris following attachment to D beta H, and were transported back to, and accumulated in, the superior cervical ganglion (SCG). Concurrent, or prior destruction of noradrenergic terminals with 6-hydroxydopamine, injected intraocularly, blocked the retrograde transport of anti-D beta H. However, recovery was rapid, reaching 50% of control values within 1 day. Such transport was characterized by a shorter time period before accumulation could be detected in the SCG and by a slower rate of accumulation. These results suggest that noradrenergic neurons recover their ability to turn over synaptic vesicles by exocytosis and transport these back to the ganglion early during the period of axonal regeneration when the axonal length is shorter than normal. The uptake and transport of anti-D beta H was regulated by alpha-adrenergic agents administered locally in the vicinity of noradrenergic nerve terminals. Thus intraocular injection of phentolamine resulted in an increased accumulation of anti-D beta H in the SCG, while amphetamine and the postsynaptic alpha-receptor antagonist, phenylephrine, decreased accumulation. Clonidine and desipramine, which have a predominant presynaptic action, failed to influence the transport of anti-D beta H. These results suggest that in vivo the uptake of anti-D beta H can be increased more by local postsynaptic reflex actions than by a mechanism depending on the inhibition of presynaptic alpha-receptors.
D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001370:Axonal Transport / Q000187:drug effects; D004299:Dopamine beta-Hydroxylase / Q000276:immunology*; D005728:Ganglia, Sympathetic / Q000187:drug effects*; D006168:Guinea Pigs; D006892:Hydroxydopamines / Q000633:toxicity; D007498:Iris / Q000294:innervation; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000187:drug effects; D009638:Norepinephrine / Q000378:metabolism*; D016627:Oxidopamine; D010646:Phentolamine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects*
D000906:Antibodies; D006892:Hydroxydopamines; D010656:Phenylephrine; D016627:Oxidopamine; D000661:Amphetamine; D004299:Dopamine beta-Hydroxylase; D009638:Norepinephrine; D010646:Phentolamine