Control by the extracellular environment of differentiation pathways in 1003 embryonal carcinoma cells: study at the level of specific intermediate filaments.
1003 is a multipotential embryonal carcinoma (EC) clonal cell line which can be induced to follow different developmental pathways by altering the composition of the culture medium. When grown in serum-containing medium the great majority of 1003 cells remain undifferentiated; they express the ECMA 7 cell-surface embryonic antigen and very low amounts of vimentin. In serum-free medium, most 1003 cells differentiate into neuroepithelial cells. The majority of these cells are still labelled with ECMA 7 antibodies. They contain higher amounts of vimentin than EC cells, but no neurofilament proteins. Neuroepithelial cells then differentiate into neurons through a stage of preneurons containing both vimentin and the 70-K neurofilament protein. Fully differentiated neurons contain 70-K neurofilament protein but no vimentin. The 200-K neurofilament protein is detected later in the neurons. Mesenchymal cells (induced by re-adding serum) express high amounts of vimentin organized in networks. Preneurons , neurons, and mesenchymal cells do not express ECMA 7 antigen.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002999:Clone Cells; D003094:Collagen / Q000032:analysis; D003470:Culture Media; D003599:Cytoskeleton / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis; D013724:Teratoma / Q000503:physiopathology*; D014746:Vimentin
D000954:Antigens, Surface; D003470:Culture Media; D007381:Intermediate Filament Proteins; D011498:Protein Precursors; D014746:Vimentin; C004818:prekeratin; D007633:Keratins; D003094:Collagen