Monoclonal antibodies against human beta-glucocerebrosidase.
Monoclonal antibodies were obtained against the membrane-bound lysosomal enzyme beta-glucocerebrosidase (acid beta-glucosidase), which is deficient in Gaucher's disease. BALB/c mice were immunized with homogeneous enzyme protein extracted from a sodium dodecyl sulphate/polyacrylamide gel. The mice were subsequently hyperimmunized with partially purified enzyme prior to fusion of spleen cells with myeloma cells. After fusion, 32 primary hybrid cell populations were obtained which continued to produce antibodies against beta-glucocerebrosidase after prolonged time of culture. All antibodies reacted with both native and denatured enzyme. Four primary cell populations were subcloned and the antibodies produced were characterized. The antibodies were all four of the IgG1 subclass. Three of these antibodies bind to protein A whereas one does not. The results of binding assays indicated that three of the antibodies react with the same antigenic domain (epitope 1), but the fourth with a different one (epitope 2). Probably two antigenic determinants are present in epitope 1 since one of the antibodies with specificity for epitope 1 is inactivated after iodination by the chloramine-T procedure whereas a second one is not.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005959:Glucosidases / Q000276:immunology*; D005962:Glucosylceramidase / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007120:Immunochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005959:Glucosidases; D005962:Glucosylceramidase