title: Actin and RNA are components of the chromatoid bodies in spermatids of the rat.
abstract: Chromatoid bodies present in spermatocytes and spermatids of the rat show directed movements around spermatid nuclei during differentiation. This transient organelle contains RNA and establishes contact to intranuclear material and to the acrosomal complex. In order to determine possible components of motility and to verify the presence of RNA, we used a recently developed low-temperature embedding resin combined with protein A-gold and enzyme-gold techniques for studies at the ultrastructural level. All chromatoid bodies analyzed display high concentrations of gold particles over the electron-dense regions when labeled with antiactin. In contrast, RNase-gold particles were localized mainly in the electron-translucent areas. Corresponding controls were always negative. The results suggest a relationship between the impressive motility of the chromatoid body and actin present in the organelle. In addition, specific localization of RNA supports earlier findings that consider the chromatoid body an essential element for differentiation during spermiogenesis.
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D009940:Organoids / Q000378:metabolism* / Q000648:ultrastructure; D012313:RNA / Q000378:metabolism*; D051381:Rats; D013087:Spermatids / Q000378:metabolism* / Q000648:ultrastructure; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D000199:Actins; D012313:RNA