title: Enzyme immunoassay for alpha-amylase.
abstract: A sensitive sandwich enzyme immunoassay has been developed for quantitation of alpha-amylase in pig pancreas. The alpha-amylase-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to hog pancreas alpha-amylase. With this method the measuring range is 1-10 ng/ml and the detection limit is 0.2 ng/ml. The amount of pig pancreatic amylase was measured to be 6.21 +/- 2.05 mg/g of tissue. The enzymatic activity determination and immunoassay were compared in the material studied.
mesh_terms: D000681:Amylases / Q000032:analysis*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D013552:Swine; D000516:alpha-Amylases / Q000032:analysis*
chemical_list: D000681:Amylases; D000516:alpha-Amylases