DNA methylation and regulation of the human beta-globin-like genes in mouse erythroleukemia cells containing human chromosome 11.
The human beta-globin gene is expressed--but the human fetal (gamma) and embryonic (epsilon) globin genes are not--in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes "global" DNA hypomethylation but selective activation of the human gamma-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D002460:Cell Line; D002906:Chromosomes, Human, 6-12 and X; D004247:DNA / Q000378:metabolism*; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D008745:Methylation; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism
D000081:Acetamides; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; C014026:hexamethylene bisacetamide; D001374:Azacitidine