Handling Golgi-impregnated tissue for light microscopy.
The use of cyanocrylic glue to fix pieces of Golgi-stained nervous tissue on a paraffin blank is proposed for obtaining thick sections of unembedded tissue with a sliding microtome. This procedure makes correct orientation of the tissue easy during sectioning and makes it possible to obtain tissue sections quickly. The sections are flat-mounted using epoxy resin, resulting in permanent preparations with excellent optical properties and enabling further thin-sectioning for light and electron microscopic studies.
D000269:Adhesives; D000818:Animals; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000166:cytology*; D004853:Epoxy Resins; D007395:Interneurons / Q000166:cytology*; D051379:Mice; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D010232:Paraffin; D013194:Staining and Labeling
D000269:Adhesives; D004853:Epoxy Resins; D010232:Paraffin