Action of a new antibiotic, neo-enactin, on the cell membrane and its application to combination treatment in a cultured tumor cell system.
Neo-enactin, a new antibiotic obtained from a Streptoverticillium, was found to act on the eukaryotic cell membrane and induce changes in the transport of molecules across the cell membrane. Neo-enactin markedly potentiated the action of the antitumor agents bleomycin and vincristine in vitro. The uptake of [14C]vincristine by HeLa cells was enhanced 3.4-fold in the presence of neo-enactin. It is suggested that the potentiation of a second drug by neo-enactin may result from enhanced permeation of that drug.
D000818:Animals; D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D006128:Growth; D006367:HeLa Cells / Q000378:metabolism / Q000502:physiology; D006801:Humans; D006877:Hydroxamic Acids; D051379:Mice; D008813:Mice, Inbred ICR; D013936:Thymidine / Q000378:metabolism; D014750:Vincristine / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D004273:DNA, Neoplasm; D006877:Hydroxamic Acids; D001761:Bleomycin; D014750:Vincristine; C019022:neo-enactin; D013936:Thymidine