T cell surface I-J glycoprotein. Concerted action of chromosome-4 and -17 genes forms an epitope dependent on alpha-D-mannosyl residues.
Two genes acting in concert control murine T cell I-Jk expression. We determined I-Jk expression with I-Jk--specific monoclonal antibodies WF8 .C12.8 and five others produced in our laboratory in a cytotoxicity assay. Previous experiments established that an H-2k gene and a chromosome 4 gene, Jt , regulate I-Jk expression. We show here that B10. HTT and B10.S( 9R ) do not differ at the H-2k locus required for I-Jk expression. Rather B10. HTT , like B10.A(3R), lacks some important non--H-2 gene (possibly Jt ). The intra--H-2k I-J--controlling locus maps to the right of the I-A subregion. The I-Jk determinant involves a carbohydrate structure associated with protein; inhibiting either protein synthesis or glycosylation prevents T cell I-Jk reexpression after proteolytic removal. Treatment with alpha-mannosidase destroys I-Jk determinants, implicating terminal alpha-D-mannosyl residues in the I-Jk epitope. Models for H-2 and Jt control of I-J expression are discussed.
D000818:Animals; D002874:Chromosome Mapping; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex; D008358:Mannose; D008361:Mannosidases / Q000494:pharmacology; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009439:Neuraminidase / Q000494:pharmacology; D014176:Protein Biosynthesis; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D043323:alpha-Mannosidase
D000939:Epitopes; D006023:Glycoproteins; D006183:H-2 Antigens; D008361:Mannosidases; D009439:Neuraminidase; D043323:alpha-Mannosidase; D008358:Mannose