title: Origin of axoplasmic RNA in the squid giant fiber.
abstract: The origin of axoplasmic RNA in the squid giant fiber was investigated after exposure of the giant axon or of the giant fiber lobe to [3H]uridine. The occurrence of a local process of synthesis was indicated by the accumulation of labeled axoplasmic RNA in isolated axons incubated with the radioactive precursor. Similar results were obtained in vivo after injection of [3H]uridine near the stellate nerve at a sizable distance from the ganglion. Exposure of the giant fiber lobe to [3H]uridine under in vivo and in vitro conditions was followed by the appearance of labeled RNA in the axoplasm and in the axonal sheath. While the latter process is attributed to incorporation of precursor by sheath cells, a sizable fraction of the radioactive RNA accumulating in the axoplasmic is likely to originate from neuronal perikarya by a process of axonal transport.
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism*; D049832:Decapodiformes; D005728:Ganglia, Sympathetic / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism
chemical_list: D012313:RNA; D014529:Uridine