Discovery, purification and characterization of the angiotensin converting enzyme inhibitor, L-681,176, produced by Streptomyces sp. MA 5143a.
L-681,176, an inhibitor of angiotensin converting enzyme was found in the culture filtrate of Streptomyces sp. MA 5143. The I50 of the crystalline inhibitor is about 1.3 micrograms/ml and the inhibition is reversed by zinc sulfate. In rats, L-681,176 exhibits a dose-related inhibition of the pressor response to angiotensin I with an ID50 of 142 mg/kg when administered intravenously. The structure of L-681,176 is similar to that of marasmine but lacking one carboxyl group. The maximum yield of L-681,176 occurs after three to four days growth at 28 degrees C.
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006146:Guanidines / Q000302:isolation & purification* / Q000494:pharmacology; D066298:In Vitro Techniques; D009842:Oligopeptides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D013302:Streptomyces / Q000378:metabolism*; D013722:Teprotide
D000806:Angiotensin-Converting Enzyme Inhibitors; D006146:Guanidines; D009842:Oligopeptides; C041574:L 681176; D013722:Teprotide