Generation of immune memory by haptenated derivatives of thymus-independent antigens in C57BL/6 mice. I. The differentiation of memory B lymphocytes into antibody-secreting cells depends on the nature of the thymus-independent carrier used for memory induction and/or revelation.
It has been previously reported that trinitrophenylated lipopolysaccharide (TNP-LPS), a thymus-independent (TI)-1 antigen, elicits an anamnestic response to TNP in C57BL/6 mice. The ability of these mice to mount a secondary response to TI-2 antigens was analyzed. Priming with DNP-Ficoll or DNP-Dextran, both TI-2 antigens, resulted in an increased frequency of TNP-binding B lymphocytes. Evidence is presented that memory cell-induction by DNP-Ficoll does not require functional T cells. The differentiation into antibody-forming cells (AFC) of memory cells generated by DNP-Dextran or DNP-Ficoll cannot be obtained by a challenge with either antigen. There was no indication that the lack of a secondary response to TI-2 antigens was related to suppressive T cells interfering with memory expression. Memory cells induced by DNP-Dextran or DNP-Ficoll can nevertheless be activated by TNP-LPS. In contrast to the restricted sensitivity of TNP-memory cells generated by TI-2 antigens, TNP-LPS-induced memory cells are indifferently susceptible to TI-1 or TI-2 antigenic stimulation. These results are discussed in terms of memory B-cell subpopulations.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology* / Q000276:immunology; D000955:Antigens, T-Independent / Q000145:classification / Q000276:immunology*; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D003911:Dextrans / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D007116:Immunization, Passive; D007156:Immunologic Memory; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*
D000955:Antigens, T-Independent; C031007:DNP-Ficoll; D003911:Dextrans; D004136:Dinitrobenzenes; D008070:Lipopolysaccharides; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D005362:Ficoll