In vivo incorporation of [32P]orthophosphate into myelin basic protein of developing rabbit brain: its location in components 3 and 5 and in a new protein tentatively identified as basic protein component 7.
Myelin was isolated from the brains of 16-day-old rabbits that had received intracerebral injections of [32P]orthophosphate 24 h earlier. The basic protein was extracted and examined by polyacrylamide gel electrophoresis both at low and at high pH. At low pH a single band corresponding to the basic protein contained all of the covalently bound protein radioactivity. At high pH, three bands of radioactivity corresponding to basic protein components 3, 5, and 7 were observed. Estimation of the specific radioactivities of the components in conjunction with their high pH electrophoretic mobilities indicated that components 3, 5, and 7 contained one, two, and three phosphate groups per molecule of protein, respectively.
D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000096:biosynthesis*; D011122:Polyphosphates / Q000378:metabolism*; D011817:Rabbits
D004676:Myelin Basic Protein; D011122:Polyphosphates