Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes.
The dye Cibacron Blue F3GA has a high affinity for many proteins and enzymes. It has therefore been attached to various solid supports such as Sephadex, Sepharose, polyacrylamide, and the like. In the immobilized form the dye has rapidly been exploited as an affinity chromatographic medium to separate and purify a variety of proteins including dehydrogenases, kinases, serum albumin, interferons, several plasma proteins, and a host of other proteins. Such a diversity shown by the blue dye in binding several unrelated classes of proteins has generated considerable work in terms of studies of the chromophore itself and also the immobilized ligand. As a prelude to realizing the full potential of the immobilized Cibacron Blue F3GA, an understanding of the basic interactions of the dye with its surroundings must be gained. It has been recognized that the dye is capable of hydrophobic and/or electrostatic interactions at the instance of the ambient conditions. The study of interactions of the dye with salts, solvents, and other small molecules indicates the nature of the interactions of the dye with different kinds of groups at the interacting sites of proteins. The review will cover such interactions of the dye with the proteins, the interactions of the proteins with the immobilized ligand, and the media used to elute the bound protein in several cases, and thus consolidate the available information on such studies into a cogent and comprehensive explanation.
D001798:Blood Proteins / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D004720:Endonucleases / Q000302:isolation & purification; D004798:Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D005420:Flavoproteins / Q000302:isolation & purification; D007372:Interferons / Q000302:isolation & purification; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D009124:Muscle Proteins / Q000302:isolation & purification; D010088:Oxidoreductases / Q000302:isolation & purification; D010770:Phosphotransferases / Q000302:isolation & purification; D011119:Polynucleotides / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship; D014227:Triazines / Q000378:metabolism*; D001618:beta-Lactamases / Q000302:isolation & purification
D001798:Blood Proteins; D004798:Enzymes; D005420:Flavoproteins; D008024:Ligands; D009124:Muscle Proteins; D011119:Polynucleotides; D011506:Proteins; D014227:Triazines; C012842:Cibacron Blue F 3GA; D007372:Interferons; D010088:Oxidoreductases; D010770:Phosphotransferases; D004720:Endonucleases; D001618:beta-Lactamases