Virus-induced interferon production in canine lymphoid cell cultures.
Virus-induced interferon (IFN) production in canine lymphoid cells was studied, using Newcastle disease virus as principal inducer. It was found that spleen cells at a concentration of 5 X 10(6) cells/ml with Newcastle disease virus at a multiplicity of infection of 1, incubated at 37 C in 5% CO2 for 24 hours, produced highest titers of IFN. Among the lymphoid cells from different tissues, IFN production was in the order of spleen = bone marrow greater than thymus greater than mesenteric lymph node greater than or equal to peripheral blood lymphocytes. Macrophages did not produce IFN, and virus-induced IFN production in spleen cells did not depend on the presence of phagocytic mononuclear cells. These optimal conditions and macrophage independence are different from those of mitogen-induced IFN production in canine lymphoid cells.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D004285:Dogs / Q000378:metabolism*; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008643:Mesentery; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D009522:Newcastle disease virus / Q000276:immunology*; D011817:Rabbits; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D007369:Interferon Inducers; D008934:Mitogens; D007372:Interferons