The effects of polycations on vascular permeability in the rat. A proposed role for charge sites.
This study investigated whether charge sites in the walls of the microvasculature may play a role in maintaining the impermeability of the nonrenal capillaries to albumin. All experiments were performed in nephrectomized rats, studied in the awake state. The intravenous injection of protamine sulfate (4 mg/100 g body wt dissolved in 0.9% saline) was followed by a mean increase of 29.1% in hematocrit and a decrease of 28.4% in plasma albumin concentration over a 10-min period, indicating a significant 50-60% loss of albumin from the vascular space; a finding confirmed by studies using exogenous 125I-labeled albumin. Changes persisted for the remaining 80 min of observation, and could be reproduced by the injection of two other polycations, hexadimethrine and poly-l-lysine. These effects were not prevented by the antihistamine diphenhydramine hydrochloride. In contrast to 125I-labeled albumin, 14C-labeled neutral dextran of comparable size was not confined to the vascular space; its apparent volume of distribution progressively increased during the 90 min of observation. Intravenous injection of protamine sulfate was followed by a significantly smaller loss of 14C-dextran (36.5%) than albumin (59.1%) from the vascular space (P less than 0.01). Protamine sulfate could not be demonstrated to result in any changes in the physicochemical characteristics of albumin. These observations suggest that the negative charge sites present in nonglomerular capillary walls have functions similar to equivalent sites present in the glomerular capillaries. Thus, charge sites could contribute to the low permeability of the microvasculature to negatively charged macromolecules such as albumin. This may be an important mechanism for retaining albumin in the vascular space and preventing edema formation in health.
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D003911:Dextrans / Q000378:metabolism; D004155:Diphenhydramine / Q000494:pharmacology; D005260:Female; D006400:Hematocrit; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D009997:Osmotic Pressure; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis / Q000378:metabolism / Q000502:physiology
D003911:Dextrans; D004155:Diphenhydramine; D006583:Hexadimethrine Bromide; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine; D011108:Polymers; D011479:Protamines; D012709:Serum Albumin