title: The two alpha 2-macroglobulin-bound trypsin molecules have different affinities for the basic pancreatic trypsin inhibitor.
abstract: We have investigated the enzymatic properties of alpha 2-macroglobulin-bound porcine trypsin using a substrate: Z-Gly-Gly-Arg-p-nitroanilide and two inhibitors: p-aminobenzamidine and basic pancreatic trypsin inhibitor. The ternary alpha 2-macroglobulin-(trypsin)2 complex behaves like a mixture of two enzymes which bind basic pancreatic trypsin inhibitor with widely different affinities (Ki = 0.11 microM and 23 microM). About one-half of the trypsin molecules of the ternary complex are covalently bound to alpha 2-macroglobulin. Preparation of the complex in the presence of hydroxylamine prevents covalent bond formation, but the two trypsins of this artificial complex still exhibit large differences in affinity for basic pancreatic trypsin inhibitor. The trypsin molecules of the ternary complex also exhibit small differences in their affinity for Z-Gly-Gly-Arg-p-nitroanilide and p-aminobenzamidine.
mesh_terms: D001550:Benzamidines / Q000378:metabolism; D006801:Humans; D008433:Mathematics; D008970:Molecular Weight; D009842:Oligopeptides / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism*
chemical_list: D001550:Benzamidines; D009842:Oligopeptides; D014361:Trypsin Inhibitors; D000511:alpha-Macroglobulins; D014359:Trypsin Inhibitor, Kazal Pancreatic; C006500:4-aminobenzamidine; C042484:benzyloxycarbonylglycyl-glycyl-arginine-4-nitroanilide; D014357:Trypsin