Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.
Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a solution of pepsin (Burns et al. (1980) Histochemistry 67:73-78) revealed the antigenicity of this basement membrane glycoprotein. Sections from paraffin blocks stored for years at room temperature could be stained with this procedure. Normal human tissues, developing fetal tissues and tumors could be stained with this method. The staining patterns were similar to those seen in unfixed frozen sections. It thus appears that basement membrane components can be detected by immunohistological means from routinely processed histological samples, once the sections are pretreated with proteases. Staining for laminin could be used in embryonic studies and in histopathology to study the relation of cells to basement membranes and for the visualization of normal and abnormal vascularization.
D000941:Antigens / Q000032:analysis*; D001485:Basement Membrane / Q000032:analysis*; D005404:Fixatives; D005557:Formaldehyde / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis*; D006652:Histological Techniques; D006801:Humans; D007797:Laminin; D013194:Staining and Labeling
D000941:Antigens; D005404:Fixatives; D006023:Glycoproteins; D007797:Laminin; D005557:Formaldehyde