[Electrophoretic study of amylase polymorphism in the ruminal contents and blood serum of sheep and heifers].
Electrophoresis of the investigated materials was carried out in a Shandou SAE soothern 2761 chamber. The amylase activity was demonstrated by the sandwich method on an agar starch substrate. A comparatively best result was obtained with the use of a Celogel carrier, followed by the cellulose-acetate folio and agar. It was found that the rumen liquid had up to 5 amylase-active zones with the use of Celogel or cellulose-acetate folio. The proper conditions for incubation of the substrate were: temperature--45 degrees C, time--1 h, and pH value--5.
D000681:Amylases / Q000032:analysis* / Q000097:blood; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D011110:Polymorphism, Genetic; D012417:Rumen / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology*; D012756:Sheep / Q000378:metabolism*; D013045:Species Specificity
D000681:Amylases
Elektroforetichno prouchvane vurkhu polimorfizma na amilazata v sudurzhanieto na turbukha i kruvniia serum ot ovtse i iunitsi.