Frequencies of phosphorylcholine-specific and T15-associated 10/13 idiotope-positive B cells within lipopolysaccharide-reactive B cells of adult BALB/c mice.
The frequencies of lipopolysaccharide-reactive B lymphocytes in adult BALB/c spleen cells were determined by limiting dilution experiments to be between 1 in 50 and 1 in 100. Within this reactive population, the frequencies of B lymphocytes specific for phosphorylcholine (PC) and/or binding a TEPC 15-associated idiotope-specific monoclonal antibody (10/13) were found to be between 1 in 50 and 1 in 500. Binding of these antibodies to immobilized PC or to monoclonal idiotope-specific antibodies could be inhibited by soluble PC in a solid-phase radioimmunoassay. Around half the PC-specific B cell clones were found to also bind 10/13 idiotope-specific monoclonal antibody, while the rest were either PC specific, but idiotope-negative or idiotope-positive, and unable to bind immobilized PC.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D002794:Choline / Q000031:analogs & derivatives*; D002999:Clone Cells / Q000378:metabolism; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007075:Immunoglobulin M / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D010767:Phosphorylcholine / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011917:Rats, Inbred Lew
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D010767:Phosphorylcholine; D002794:Choline