Isolation and elution of proteins from sodium dodecyl sulfate-polyacrylamide gels with an intermediate agarose-containing layer in the acrylamide gel.
D000510:Alpha-Globulins / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005450:Fluorescamine; D007114:Immunization; D007122:Immunoelectrophoresis; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D013997:Time Factors
D000510:Alpha-Globulins; D000941:Antigens; D011506:Proteins; C016001:alpha-1-microglobulin; D005450:Fluorescamine