Antigen-specific helper activity in serum of mice primed with sheep red cells II. Partial purification of the factors involved.
The nature of antigen-specific, T-cell dependent helper activity (SSHA), present in serum of mice 9 h after intraperitoneal immunization with 2 x 10(8) sheep erythrocytes, was studied in more detail. SSHA could be enriched from serum by adsorption to formalinized sheep erythrocytes, elution of the cells with a chaotropic substance, and subsequent dialysis. This resulted in an enrichment of about 900 x with a yield of 64%. Further fractionation of the material by ultrafiltration resulted in two subfractions with helper activity indicating SSHA to be composed of two factors with mol. wt's of greater than 300,000 and about 60,000. The eventual purification for the two factors was about 1650 and 2000 x respectively, and the total yield 46%. The possible relationship between the factors and factors described by others after induction in vitro is discussed. The purification procedure appears useful for the enrichment of SRBC-specific helper factors from serum.
D000818:Animals; D005591:Chemical Fractionation; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D007163:Immunosorbent Techniques; D007376:Interleukin-2 / Q000032:analysis / Q000302:isolation & purification*; D007519:Isoantigens / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011817:Rabbits; D012756:Sheep; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014462:Ultrafiltration
D000939:Epitopes; D007376:Interleukin-2; D007519:Isoantigens