Lack of correlation between morphological index and viability as assessed by the uptake of 3H-thymidine by macrophage resident M. leprae.
Thirty strains of M. leprae derived from skin biopsies of lepromatous leprosy patients were scored for Morphological Index (MI) and concurrently maintained for 2 weeks in macrophage cultures containing 3H thymidine. Selective and significant incorporation of the radioactive label was observed in cultures containing freshly extracted M. leprae as compared to control cultures with autoclaved bacilli from the same biopsy. The percentage incorporation of 3H thymidine ranged from 103 to 1140%. Morphological Index of the bacilli from these individuals varied from zero to 8. Six cultures containing bacilli with MI of less than or equal to 1 and 3 containing bacilli with MI of zero showed significant incropration of 3H thymidine. There was no correlation between the percent of solid or beaded bacilli in the inoculum and the ability of M. leprae to incorporate 3H thymidine in the macrophage cultures.
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D009166:Mycobacterium leprae / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*; D013194:Staining and Labeling; D013936:Thymidine / Q000378:metabolism*
D013936:Thymidine