Conjugated dimers of horseradish peroxidase as neuroanatomical tracers.
Horseradish peroxidase dimer (HRP-HRP) and monomer (HRP) were compared in their ability to act as neuroanatomical tracers. Dimers exhibited less diffusion at the site of injection. At 48-72 h survival periods, there were no appreciable differences in the retrograde neuronal labeling produced by these two compounds. However, at 7 days survival, there was little retrograde labeling due to the presence of monomer while dimer labeling was still intense.
D000818:Animals; D001370:Axonal Transport; D004525:Efferent Pathways / Q000502:physiology; D006735:Horseradish Peroxidase; D037102:Lectins; D046911:Macromolecular Substances; D008970:Molecular Weight; D009420:Nervous System / Q000033:anatomy & histology*; D009474:Neurons / Q000502:physiology*; D010544:Peroxidases; D051381:Rats; D013053:Spectrophotometry; D014909:Wheat Germ Agglutinins
D037102:Lectins; D046911:Macromolecular Substances; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase; D010544:Peroxidases