Effect of phorbol ester TPA on macrophage metabolic activity.
Metabolically active macrophages are known to play a role in tumor immune surveillance, not only as effector cells but also as collaborators in T cell mediated immune response. The present work reports that non-toxic concentrations of the tumor promoter TPA, stimulate the adherence of murine peritoneal macrophages after short time incubation, while TPA pretreatment for periods longer than 4 hrs suppresses phagocytosis, RNA and protein synthesis by these cells. Although inhibition of macrophage metabolic activity could play an additional role in tumor promotion, the enhancing and depressing effects of TPA on the immune system must be weighed before it can be assumed that TPA acts in vivo primarily to depress the immune system.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003470:Culture Media; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D010704:Phorbols / Q000633:toxicity*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D013997:Time Factors; D014343:Trypan Blue / Q000633:toxicity; D014529:Uridine / Q000378:metabolism
D003470:Culture Media; D010704:Phorbols; D012313:RNA; D007930:Leucine; D014343:Trypan Blue; D013755:Tetradecanoylphorbol Acetate; D014529:Uridine