Expression of a chemically synthesized human alpha 1 interferon gene.
Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D001681:Biological Assay; D004274:DNA, Recombinant; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000235:genetics* / Q000494:pharmacology; D008772:Methylococcaceae / Q000235:genetics; D010957:Plasmids
D004274:DNA, Recombinant; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase