Prevention of granuloma development in the mouse by using T cell hybridoma products.
The ability of an azobenzenearsonate (ABA)-specific suppressor T cell factor, a soluble extract from first order suppressor T cells (Ts1), and suppressor molecules produced by a long-term T cell hybridoma to regulate ABA-specific granuloma formation was studied. ABA-derivatized syngeneic spleen cells (ABA-SC) administered subcutaneously induced persistent delayed-type hypersensitivity (DTH) responses, detected by footpad swelling and hapten-specific granuloma formation by 72 and 96 hr after challenge with ABA-bovine serum albumin coupled to polyacrylamide beads (ABA-BSA-PAB). Soluble factors from ABA-specific Ts1 prevented DTH and granulomatous development after subcutaneous administration of ABA-SC. Moreover, the in vivo administration of a factor that is derived from a Ts1 functioning hybrid cell line induced a second set of suppressor cells (Ts2) that upon transfer to syngeneic ABA-primed mice were able to inhibit granuloma formation in the footpad, as well as in the gastrointestinal tract after challenge with ABA-BSA-PAB. These experiments demonstrate the dependence of the granulomatous reaction on T cell-mediated events, as well as the potential therapeutic efficacy of an antigen-specific suppressor T cell factor and a hybridoma T cell product in limiting antigen-specific granuloma formation in vivo.
D000818:Animals; D004195:Disease Models, Animal; D000939:Epitopes; D005260:Female; D006099:Granuloma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000628:therapy; D008222:Lymphokines / Q000008:administration & dosage*; D051379:Mice; D008805:Mice, Inbred A; D013491:Suppressor Factors, Immunologic; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D010132:p-Azobenzenearsonate / Q000276:immunology
D000939:Epitopes; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D010132:p-Azobenzenearsonate