Expression of gastric-associated antigens by human premalignant and malignant colonic epithelial cells.
A rabbit antiserum prepared to 2nd-trimester fetal organ extracts and absorbed with adult tissue (anti-STFa) was used to detect antigens common to 2nd-trimester fetal large bowel, normal adult stomach, several colon carcinoma cells (in primary and established cultures) and epithelial cells cultured from benign colonic polyps. The frequency of anti-STFa-positive cells was highest in cultures derived from benign tumors known to be associated with a greater degree of premalignancy. Thus, the percentage of antigen-positive cells increased from 18 and 43% to 70% of cultures from tubular, villotubular and villous adenomas, respectively. Considerable heterogeneity in the distribution of antigen-containing cells was evident within any given area of a positive culture. Absorption experiments, using a spectrum of fetal and normal adult tissue extracts, indicated that the adenoma-carcinoma specificity resides in fetal, but not adult, large bowel and normal adult stomach.
D000236:Adenoma / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005260:Female; D005333:Fetus / Q000276:immunology; D006801:Humans; D011127:Polyps / Q000276:immunology; D011230:Precancerous Conditions / Q000276:immunology*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D013270:Stomach / Q000276:immunology*
D000951:Antigens, Neoplasm; D000939:Epitopes