[Attempt to isolate from Ascaris suum an antigenic fraction for use in the immunologic diagnosis of onchocerciasis].
For the purpose of immunological diagnosis of onchocerciasis, the authors attempted to isolate an antigenic fraction from Ascaris suum which would only detect anti-Onchocerca volvulus antibodies. Chromatofocusing was done on a crude antigenic preparation from the ascaris. Immunoelectrophoresis showed that the fraction eluted between pH 5.6 and pH 4.9 contained antigens which reveal anti-Onchocerca antibodies; anti-Loa loa antibodies are revealed by the fraction eluted at pH less than 4. From the fraction which reveal anti-Onchocerca antibodies, affinity chromatography isolated a system in which three components were detected by electrofocusing at a Pi of about pH 5.5. The specificity and sensitivity of this system are being studied.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D001200:Ascaris / Q000276:immunology*; D002846:Chromatography, Affinity; D000939:Epitopes; D005260:Female; D007525:Isoelectric Focusing; D008297:Male; D009855:Onchocerciasis / Q000175:diagnosis*
D000941:Antigens; D000939:Epitopes
Essai d'isolement, à partir d'Ascaris suum, d'une fraction antigénique en vue du diagnostic immunologique de l'onchocercose.