title: Myelin basic protein: a component of circulating immune complexes in multiple sclerosis.
abstract: Myelin basic protein (MBP) is an antigenic component of circulating immune complexes (CIC) in patients with multiple sclerosis (MS). Immune complexes were isolated from the sera by adsorption to Raji cells and then acid eluted. Final identification of MBP from Raji eluates was done by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by MBP radioimmunoassay (RIA) of gel eluates and by an immunoblot technique.
mesh_terms: D000936:Antigen-Antibody Complex / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009103:Multiple Sclerosis / Q000276:immunology*; D004676:Myelin Basic Protein / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D012008:Recurrence
chemical_list: D000936:Antigen-Antibody Complex; D004676:Myelin Basic Protein