Activation and proliferation signals in mouse B cells. I. A comparison of the capacity of anti-Ig antibodies or phorbol myristic acetate to activate B cells from CBA/N or normal mice into G1.
B lymphocytes from the CBA/N mouse do not synthesize DNA when cultured with anti-Ig antibodies. However, these cells like normal B cells, do manifest increased Ia antigen expression and RNA synthesis (i.e. enter G1) when stimulated by anti-Ig, even at doses which are nonmitogenic for normal B cells. Pretreatment of both normal and CBA/N B cells with anti-Ig also primes them to give an enhanced proliferative response to lipopolysaccharide (LPS). The tumor promoter phorbol myristic acetate (PMA) also enhances RNA synthesis and Ia antigen expression in B cells from both normal and CBA/N mice. However, PMA only primes CBA/N B cells to respond to LPS: pretreatment of normal B cells with PMA causes a modest suppression of LPS-induced, and a marked suppression of anti-Ig induced, DNA synthesis. These results therefore confirm and extend earlier data showing that there are distinct activating (G0 to G1) vs. proliferative (G1 to S) signals discernible in B cells. They also suggest that the activation mechanism of CBA/N B cells is subtly different from that of any known subpopulation of normal B cells.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007399:Interphase / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010704:Phorbols / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000888:Antibodies, Anti-Idiotypic; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D010704:Phorbols; D012313:RNA; D004247:DNA; D013755:Tetradecanoylphorbol Acetate