Detection of specific murine anti-tumour immunity and serum factors by the indirect E-rosette augmentation assay.
The spontaneous interaction of sheep erythrocytes and human lymphocytes results in the formation of aggregates known as E-rosettes. The antigen reactivity of murine T-lymphocytes can be detected indirectly by the production of a lymphokine that augments the E-rosette-forming ability of human lymphocytes. An indirect E-rosette augmentation (ERA) assay was used to study cellular reactivity and tumour-related serum reactions in mice bearing methylcholanthrene (MCA)-induced tumours. The assay was used to demonstrate the antigenic specificity of two tumours and to detect blocking factors in tumour-bearer serum. Blocking factors were shown to be tumour-specific and to act on lymphocytes at an early stage before exposure to antigen.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001667:Binding, Competitive; D001798:Blood Proteins / Q000502:physiology; D000939:Epitopes / Q000032:analysis*; D005354:Fibrosarcoma / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008077:Lipoproteins, LDL; D051379:Mice; D008808:Mice, Inbred CBA; D009374:Neoplasms, Experimental / Q000276:immunology; D012397:Rosette Formation
D000951:Antigens, Neoplasm; D001798:Blood Proteins; D000939:Epitopes; D008077:Lipoproteins, LDL; C022060:rosette-inhibiting factor