Detection and characterization of idiotype-specific enhancing cells generated in mice immunized with idiotype.
Cellular interaction between MOPC-104E (M104E) cross-reactive idiotypic (CRI) antibody-producing B lymphocytes and lymphocytes generated by immunization with the relevant idiotype, M104E, was investigated. Adoptive transfer of M104E idiotype-primed and normal spleen cells into 600R x-irradiated syngeneic recipient mice resulted in striking enhancement of the M104E-CRI positive antibody response upon simultaneous immunization of recipients with dextran B1355S. The enhancement was not attributable to a simple additive effect but was due to synergistic cooperation between the two lymphocyte populations. This synergistic enhancement of the anti-idiotype immune cells producing CRI antibody was specific for MOPC-104E CRI, and was reproducible in an in vitro culture system. Because of the cellular characteristics of the enhancing cells, they were assumed to be B lymphocytes specific for the corresponding idiotype, since the activity was not abrogated by treatment with anti-Thy-1, anti-Lyt-1, anti-Lyt-2, or anti-brain-associated theta antisera plus complement, but was eliminated by means of a planning method using a rabbit-anti-mouse immunoglobulin-coated or idiotype-coated dish. The mechanisms of interaction between the CRI-positive B cells and anti-idiotypic B cells in response to the thymus-independent antigen dextran B1355S are discussed.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D003429:Cross Reactions; D003911:Dextrans / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000008:administration & dosage / Q000276:immunology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D003911:Dextrans; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins; D018800:Thy-1 Antigens