A newly defined murine alloantigen controlled by the S region of the H-2 complex: molecular association with the fourth component of complement.
A previous study has shown that prolonged immunization of strain A.TBR16 mice (H-2 haplotype at16) with lymphoid tissue derived from A.TBR13 (at13) donors results in an antiserum that defines a new H-2-associated allotype with a strain distribution antithetical to H-2.7. The present report describes a sensitive ELISA that demonstrated that this specificity represents an allotypic determinant(s) on the fourth component of complement (C4). Studies with Sephadex G-200 fractionated plasma and serum proteins suggest that, as is the case for H-2.7, the new specificity appears to reside on the C4d fragment after C activation. We therefore propose the tentative nomenclature C4d.2 for this specificity and suggest revision of the nomenclature for H-2.7 to C4d.1. Also described in this report is the formal mapping of the genetic control of the C4d.2 allotype to the H-2S region.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001609:Beta-Globulins / Q000235:genetics* / Q000276:immunology; D003181:Complement C4 / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000032:analysis* / Q000235:genetics; D006386:Hemagglutination Tests; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013045:Species Specificity
D000961:Antilymphocyte Serum; D001609:Beta-Globulins; C016759:C4b protein, mouse; D003181:Complement C4; D006183:H-2 Antigens