Antibiotic uptake by bacteria as measured by partition in polymer aqueous phase systems.
Polyethyleneglycol/dextran and polyethyleneglycol/salt two-phase systems are used to measure the entry of antibiotics into Escherichia coli and Staphylococcus aureus cells. Aminoglycosides, macrolides, a quinolone, and a cycline were assayed. The method is simple and rapid and eliminates the problems encountered with filters, especially nonspecific binding; it allows rapid uptake kinetics to be measured.
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D003911:Dextrans; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008278:Magnesium Sulfate; D010710:Phosphates; D011092:Polyethylene Glycols; D012995:Solubility; D013211:Staphylococcus aureus / Q000378:metabolism; D013696:Temperature
D000900:Anti-Bacterial Agents; D003911:Dextrans; D010710:Phosphates; D011092:Polyethylene Glycols; D008278:Magnesium Sulfate; C018279:sodium phosphate