Immunochemical determinant of Candida parapsilosis.
Acetolysis of Candida parapsilosis cell-wall D-mannan, obtained by alkali extraction and purified as a copper complex, gave six oligosaccharides by Bio-Gel P-2 filtration. Their chemical structure was examined by 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis. The structures of the penta- and hexa-saccharide were alpha-D-Manp-(1 leads to 3)-alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 2)-D-Man (M5) and alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 3)-alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 2)-D-Man (M6), respectively. Inhibition of the oligosaccharides with anti-C. parapsilosis serum and homologous D-mannan indicated that although M5 and M6 exhibited strong inhibitory-activity, M6 was the more effective inhibitor, and suggested that M6 may be the immunodominant or may be responsible for the specificity of C. parapsilosis mannan in the precipitin-reaction system, or both.
D000954:Antigens, Surface / Q000302:isolation & purification*; D002175:Candida / Q000032:analysis / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification*; D013045:Species Specificity
D000954:Antigens, Surface; D000939:Epitopes; D008351:Mannans; D011134:Polysaccharides