A factor shed by lymphoblastoid cell lines of HLA-B27 positive patients with ankylosing spondylitis, specifically modifies the cells of HLA-B27 positive normal individuals.
Epstein-Barr virus transformed lymphoblastoid cell lines from HLA-B27 positive individuals with ankylosing spondylitis (B27+AS+) release, into the culture medium, a factor capable of specifically modifying the HLA-B27 positive lymphocytes of normal individuals (B27+AS-); this modification results in a phenotypic change similar to that seen on B27+AS+ lymphocytes. This lymphoblastoid cell line derived factor appears to be physically and functionally similar to a factor present in the culture filtrate of certain Klebsiella isolates. Biogel P-100 chromatography of the material released from the cell line indicated a mol.wt of 25,000-30,000, similar to that of the Klebsiella derived factor. Chromatofocusing on a PBE 94 column revealed that cell line derived factor had an isoelectric point of 5.5 (cf. pI 5.4 for the Klebsiella derived factor). Immunoadsorption experiments suggest that the factor from the B27+AS+ cell line shares antigenic determinants with a cell surface component present on certain Klebsiella isolates. These results will form the basis for future studies on the nature of the interaction between HLA-B27 and certain enteric organisms and their products. A better understanding of this association should elucidate some of the early events in the pathogenesis of the seronegative arthropathies.
D002460:Cell Line; D002472:Cell Transformation, Viral; D002850:Chromatography, Gel; D000939:Epitopes / Q000032:analysis; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D004854:Herpesvirus 4, Human; D006801:Humans; D007711:Klebsiella pneumoniae / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008970:Molecular Weight; D010641:Phenotype; D013167:Spondylitis, Ankylosing / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D015796:HLA-B27 Antigen