HLA-D/DR restriction of proliferative T cell responses to antigen. Enrichment of antigen-specific T cell blasts and evidence of preferential HLA-D/DR restriction.
Antigen-specific T cell blasts may be separated from alloreactive cells on Percoll gradients. The absence of alloreactivity allowed us to study HLA-D/DR restriction of proliferative T cell responses towards PPD and HSV antigens using allogeneic antigen-presenting cells. An overriding impact of self HLA-D/DR determinants as restriction elements for in vivo sensitized T cells was demonstrated. Furthermore, evidence was obtained that the proliferative PPD response might be preferentially restricted by HLA-D/DR 1.
D000941:Antigens / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D002469:Cell Separation; D000939:Epitopes; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D018139:Simplexvirus / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
D000941:Antigens; D000956:Antigens, Viral; D000939:Epitopes; D006684:HLA-DR Antigens; D007376:Interleukin-2; D014373:Tuberculin