Rapid and simple method for the simultaneous determination of 3,4-dihydroxyphenylacetic acid, 5-hydroxyindole-3-acetic acid and 4-hydroxy-3-methoxyphenylacetic acid in human plasma by high-performance liquid chromatography with electrochemical detection.
A simple procedure for the simultaneous determination of major metabolites of dopamine and serotonin in plasma, i.e. 3,4-dihydroxyphenylacetic acid, 5-hydroxyindole-3-acetic acid, and 4-hydroxy-3-methoxyphenylacetic acid, was developed. The method is based on rapid isolation of the compounds by one-step clean-up on a small C18 column, followed by high-performance liquid chromatography with dual electrochemical detection. The system is readily used for clinical applications.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000097:blood*; D001679:Biogenic Amines / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006719:Homovanillic Acid / Q000097:blood*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood*; D007536:Isomerism; D010648:Phenylacetates / Q000097:blood*
D001679:Biogenic Amines; D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid