Orthochromatic and metachromatic staining reactions by pyronin Y on Epon semithin sections.
Semithin sections from glutaraldehyde-fixed, Epon-embedded tissues were stained by aqueous solutions of pyronin Y at increasing concentrations (from 10(-6) to 10(-3) mol). Mucopolysaccharide containing structures (e.g. mucin) were found stained in orange, meanwhile the chromatin and remaining tissue components appeared in a bright pink-red color. Cytophotometric measurements showed that a metachromatic shift occurs in the mucin content from goblet cells after pyronin Y staining at 10(-3) mol. Some features of the metachromatic reactions by cationic dyes are briefly discussed.
D000818:Animals; D002843:Chromatin / Q000032:analysis*; D004330:Drosophila; D004853:Epoxy Resins; D007420:Intestine, Large / Q000648:ultrastructure; D051379:Mice; D009077:Mucins / Q000032:analysis*; D011754:Pyronine; D012469:Salivary Glands / Q000648:ultrastructure; D012867:Skin / Q000648:ultrastructure; D013053:Spectrophotometry; D013194:Staining and Labeling / Q000379:methods*; D014059:Tongue / Q000648:ultrastructure; D014966:Xanthenes
D002843:Chromatin; D004853:Epoxy Resins; D009077:Mucins; D014966:Xanthenes; C004875:EPON; D011754:Pyronine