title: Hepatic microsomal p-nitroanisole O-demethylase. Effects of chlordecone or mirex induction in male and female rats.
abstract: The effects of chlordecone (CD) or mirex treatment on the hepatic microsomal monooxygenase system of male and female rats were investigated, and kinetic parameters (apparent Vmax and apparent Km) for p-nitroanisole O-demethylase were studied in detail. Both pesticides elevated the levels of cytochrome P-450 in a time- and dose-dependent manner. The maximum rate of p-nitroanisole metabolism in males was increased about 100 and 50% and the apparent Km was elevated about 40- and 18-fold by CD and mirex respectively. p-Nitroanisole metabolism in females was reduced slightly by treatment with either agent, and the apparent Km was increased about 14-fold by CD but was relatively unaffected by mirex treatment.
mesh_terms: D000818:Animals; D000840:Anisoles / Q000378:metabolism; D007631:Chlordecone / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004790:Enzyme Induction; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D008917:Mirex / Q000633:toxicity*; D009575:Nitroanisole O-Demethylase / Q000096:biosynthesis*; D010088:Oxidoreductases / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000840:Anisoles; D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System; D009575:Nitroanisole O-Demethylase; D010088:Oxidoreductases; C005306:4-nitroanisole; D007631:Chlordecone; D008917:Mirex