Monoclonal antibody to murine neutrophils: identification of the Gm-2.2 specificity.
A neutrophil-specific alloantigen (Gm-2.2) was defined by a monoclonal antibody, 5119-4/7. The Gm-2.2 antigen is found only on bone marrow neutrophils or calcium caseinate-induced neutrophils and is absent from all lymphoid cells examined as well as adherent thioglycollate-induced peritoneal exudate cells and the nonhemopoietic tissues, kidney, liver, heart, brain, and red blood cells. Furthermore, unlike mature neutrophils, granulocyte/macrophage progenitor cells are Gm-2.2-, suggesting that Gm-2.2 is a differentiation antigen for the neutrophil series. The Gm-2 locus is linked to, but distinct from, the Ly-6 locus.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000950:Antigens, Ly / Q000032:analysis; D003114:Colony-Forming Units Assay; D000939:Epitopes; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology; D006183:H-2 Antigens / Q000032:analysis; D007519:Isoantigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008814:Mice, Inbred NZB; D009504:Neutrophils / Q000276:immunology*; D010641:Phenotype; D011995:Recombination, Genetic; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000939:Epitopes; D006183:H-2 Antigens; D007519:Isoantigens