Peroxidase immunocytochemistry for the detection of specific anti-hapten (penicilloyl) antibody producing cells in the spleen, after injection of a hapten-carrier conjugate.
Cells producing specific antibodies against the hapten penicilloyl (Pen) are demonstrated in tissue sections of the spleen, using peroxidase immunocytochemistry. Horseradish peroxidase-human serum albumin-penicilloyl (HRP-HSA-Pen) conjugate, prepared by coupling Pen to HRP-HSA conjugate, was used for detection of anti-Pen producing cells in the spleen after intravenous injection of bovine gamma globulin-penicilloyl (BGG-Pen) conjugate. HRP-HSA conjugate was used as a control to exclude the possibility that positive cells contained antibody against a common determinant in both HSA and BGG. The method may be applied for the detection of cells producing antibodies against other haptens as well.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000921:Antibody-Producing Cells / Q000032:analysis*; D044482:Benzeneacetamides; D006241:Haptens / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology; D007124:Immunoenzyme Techniques; D010397:Penicillanic Acid / Q000276:immunology* / Q000031:analogs & derivatives; D011817:Rabbits; D013154:Spleen / Q000166:cytology*; D005719:gamma-Globulins / Q000276:immunology*
D000906:Antibodies; D044482:Benzeneacetamides; D006241:Haptens; C041936:bovine gamma globulin-penicilloyl; D005719:gamma-Globulins; D010397:Penicillanic Acid; D006735:Horseradish Peroxidase