Sandwich enzyme immunoassay of aprotinin.
Enzyme immunoassay, using the rabbit antiaprotinin Fab'-beta-D-galactosidase complex and the rabbit antiaprotinin IgG-coupled polystyrene ball as a solid phase was used to measure sera levels of aprotinin. The least measurable amount of the aprotinin in the enzyme immunoassay was bout 0.002-0.005 kallikrein inhibitor unit/ml. In the enzyme immunoassay of aprotinin in serum, the influence of serum on the assay was eliminated by using a buffer with gelatin as a sample diluent in the first step of the antigen and antibody reaction. Aprotinin level in the serum, as estimated by enzyme immunoassay correlated highly with levels obtained by inhibition assay.
D000818:Animals; D007611:Aprotinin / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000097:blood / Q000276:immunology; D002021:Buffers; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D001616:beta-Galactosidase / Q000032:analysis
D002021:Buffers; D007074:Immunoglobulin G; D007611:Aprotinin; D001616:beta-Galactosidase