[Isolated hepatocytes fixed on collagen, an accurate model for the the secretion of proteins in a minimal medium. Response to inflammation].
The albumin, orosomucoïd and alpha 2-macroglobulin secretion by isolated hepatocytes of normal and suffering from Turpentine-induced inflammation rats, is investigated for 4 hr. The model, stable over the whole duration of incubation, is a true reflect of hepatic secretion in vivo and can be used to measure it.
D000818:Animals; D003094:Collagen; D003470:Culture Media; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008954:Models, Biological; D009961:Orosomucoid / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism*; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000378:metabolism*
D003470:Culture Media; D009961:Orosomucoid; D012709:Serum Albumin; D000511:alpha-Macroglobulins; D003094:Collagen; D014425:Turpentine
L'hépatocyte isolé fixé sur collagène, modèle fidèle pour l'étude de la sécrétion des protéines en milieu minimal. Réponse à l'inflammation.