Cellular localization of GM1-ganglioside with biotinylated choleragen and avidin peroxidase in primary cultured cells from rat brain.
A new technique capable of demonstrating the presence and cellular localization of the ganglioside GM1 in primary cultured cells from the brains of newborn rats is described. The method is based on the highly specific binding of biotinylated choleragen to ganglioside GM1, and takes advantage of the high affinity of avidin for biotin. Thus, the biotinylated choleragen-ganglioside GM1 complex can be visualized by the use of avidin peroxidase. The results of this nonimmunologic method indicate that the concentration of ganglioside GM1 is much lower in culture astroglial cells than in neurons and oligodendroglial cells.
D000818:Animals; D000831:Animals, Newborn; D001360:Avidin; D001710:Biotin; D001923:Brain Chemistry; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000032:analysis* / Q000378:metabolism; D005732:Gangliosides / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D009457:Neuroglia / Q000032:analysis*; D009836:Oligodendroglia / Q000032:analysis* / Q000378:metabolism; D010544:Peroxidases; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D005732:Gangliosides; D001360:Avidin; D005677:G(M1) Ganglioside; D001710:Biotin; D002772:Cholera Toxin; D010544:Peroxidases