A micromethod for absorption of specific antibody using an enzyme-linked immunosorbent assay (ELISA).
A procedure for absorbing specific antibodies using an enzyme-linked immunosorbent assay system (ELISA) has been developed. This is accomplished by serial absorption of the serum using less than 20 micrograms antigen. The sera can then be used in an ELISA system to test reactivity with other antigens. The system was tested by absorbing anti-myelin or anti-cerebroside antibodies and comparing these results with bulk absorption.
D000042:Absorption; D000906:Antibodies / Q000302:isolation & purification*; D000941:Antigens / Q000032:analysis*; D001923:Brain Chemistry; D002554:Cerebrosides / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005699:Galactosylceramides / Q000032:analysis*; D006801:Humans; D008832:Microchemistry; D004676:Myelin Basic Protein / Q000032:analysis*; D009186:Myelin Sheath / Q000032:analysis
D000906:Antibodies; D000941:Antigens; D002554:Cerebrosides; D005699:Galactosylceramides; D004676:Myelin Basic Protein