title: Determination of low molecular weight heparin in clinical laboratory.
abstract: The applicability was investigated of automated spectrophotometric heparin assays and three clotting assays for determination of two low molecular weight (LMW) heparin fractions: Org 10172 and DxN10 and two infractionated commercially available heparins. The relative activity of the two commercially available heparins was similar in the anti-Xa assay, in the anti-IIa assay and in 3 clotting assays. The LMW heparins showed markedly different relative activity in all 5 assays. The activities of those heparin preparations relative to the standard heparin were compared in the 5 assays, but standardization against a standard heparin preparation appeared impossible. Methods of heparin determination can be used to monitor treatment with a heparin preparation only if the same preparation is used as a reference substance.
mesh_terms: D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D004151:Dipeptides; D006025:Glycosaminoglycans / Q000032:analysis / Q000097:blood; D006493:Heparin / Q000032:analysis* / Q000097:blood; D006496:Heparinoids / Q000032:analysis; D006497:Heparan Sulfate; D006801:Humans; D008970:Molecular Weight; D009842:Oligopeptides; D010314:Partial Thromboplastin Time; D012016:Reference Values; D013053:Spectrophotometry
chemical_list: D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D004151:Dipeptides; D006025:Glycosaminoglycans; D006493:Heparin; D006496:Heparinoids; D006497:Heparan Sulfate; D009842:Oligopeptides; C015608:benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide; C021129:S 2238; C035838:danaparoid