Fast atom bombardment-collision activated dissociation-linked field scanning mass spectrometry of the neuropeptide substance P.
Amino acid sequence-determining information is obtained from nanomole amounts of the underivatized, biologically important peptide substance P by combining fast atom bombardment, collision activated dissociation, and linked field scanning mass spectrometry. Protonated molecular ions of substance P are produced by fast atom bombardment mass spectrometry, accelerated to high translational energy (8 kV), and transit a collision chamber. Collision activated dissociations occur in the first field-free region. Amino acid sequence-determining ions are collected by scanning the magnetic and electric fields, keeping their ratio constant. In this manner, the precursor-product relationship among ions produced during fragmentation of the protonated molecular ion is firmly established.
D000595:Amino Acid Sequence; D004563:Electrochemistry; D013058:Mass Spectrometry / Q000379:methods; D011522:Protons; D013373:Substance P / Q000032:analysis*
D011522:Protons; D013373:Substance P