Progenitor cell (CFUc) reconstitution after autologous stem cell transfusion in lethally irradiated dogs: decreased CFUc populations in blood and bone marrow correlate with the fraction mobilizable by dextran sulphate.
The present study in dogs concerns the functional potentials of the myeloid progenitor cell system during hemopoietic recovery after lethal total-body irradiation and autotransfusion of cryopreserved stem cells derived from peripheral blood and bone marrow. CFUc were assayed in peripheral blood and bone marrow before and at various intervals after grafting. In addition, the net increase of CFUc mobilizable from extravascular sites into the blood was determined after i.v. injection of 15 mg/kg body weight of dextran sulphate (DS). The data indicate a sustained deficiency of CFUc in otherwise hematologically normal recipients. The DS-response was subnormal even 225 days after transplantation, suggesting residual damage of the hemopoietic system is present for a prolonged period of time. A significant correlation was found between the actual blood CFUc concentration and the numbers of CFUc mobilizable by DS. Insight into progenitor cell kinetics and corresponding changes in the size of a mobilizable pool in extravascular sites may be helpful in estimating the marrow reserve of transplanted individuals suffering myelosuppressive effects of chemotherapy.
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D001854:Bone Marrow Cells; D002452:Cell Count; D002455:Cell Division; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007700:Kinetics; D007958:Leukocyte Count; D008297:Male; D014916:Whole-Body Irradiation
D003911:Dextrans; D016264:Dextran Sulfate