Messenger RNA activities of four acute phase proteins during inflammation.
Poly(A)+ RNA isolated from the livers of normal rats and of rats suffering from an acute inflammation was translated in a cell-free translation system from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against alpha 1-acid glycoprotein, alpha 2-macroglobulin, transferrin, alpha 1-proteinase inhibitor and albumin. 15 to 21 h after intramuscular injection of turpentine 73-, 66-, 2.8-, and 2-fold increases in translatable mRNAs for alpha 1-acid glycoprotein, alpha 2-macroglobulin, transferrin and alpha 1-proteinase inhibitor, respectively, were observed. For albumin a decrease in translatable mRNA to about 30% of controls was measured.
D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D002474:Cell-Free System; D007249:Inflammation / Q000378:metabolism*; D008099:Liver / Q000293:injuries / Q000378:metabolism; D008297:Male; D009961:Orosomucoid / Q000235:genetics*; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000235:genetics*; D014425:Turpentine; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins / Q000235:genetics*
D001798:Blood Proteins; D009961:Orosomucoid; D012333:RNA, Messenger; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D011061:Poly A; D012313:RNA; D014425:Turpentine