The role of nuclear serine proteases in the degradation of newly synthesized histones and ribosomal proteins.
To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried out with serine protease inhibitor, PMSF. The following results were obtained. When normal rats received an intraperitoneal injection of PMSF (10 mg per 100 g body weight), nuclear serine proteases were inhibited almost completely for at least 90 min. PMSF did not affect the synthesis of proteins and RNAs of ribosomes and other subcellular fractions. The effects of PMSF treatment in vivo on the degradation of newly synthesized ribosomal proteins and histones in regenerating rat liver pretreated with a low dose of actinomycin D, which preferentially inhibited rRNA synthesis, were examined by using the double-isotope method. It was found that PMSF treatment did not affect their degradation. On the other hand, administration of E-64, a thiol protease inhibitor, to partially hepatectomized rats inhibited the degradation of those proteins markedly. From these results, it is concluded that the nuclear thiol protease, but not serine proteases, is preferentially involved in the degradation of newly synthesized ribosomal proteins and histones which are not associated with rRNA and DNA, respectively.
D000818:Animals; D002843:Chromatin / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012269:Ribosomal Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases
D002843:Chromatin; D006657:Histones; D012269:Ribosomal Proteins; D010664:Phenylmethylsulfonyl Fluoride; D012313:RNA; D010450:Endopeptidases; D012697:Serine Endopeptidases; D007930:Leucine; C024974:E 64