Monoclonal antibodies against human low density lipoprotein. Stoichiometric binding studies using Fab fragments.
Because of its physical properties, apolipoprotein B (apo B) has remained poorly characterized. In an attempt to elucidate apo B structure, the Fab fragments of 3 different monoclonal anti-human apo B antibodies were tested in a quantitative assay for their binding to human low density lipoprotein (LDL). In each case the assay gave a linear Scatchard plot with a maximum of 1 Fab fragment bound to a single LDL particle. This result favors an LDL model containing a single large Mr apo B protein, which is not composed of multiple, identical, small Mr subunits.
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000939:Epitopes; D006801:Humans; D006825:Hybridomas; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing; D008077:Lipoproteins, LDL / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008077:Lipoproteins, LDL