Monoclonal antibody against the N-terminal end of human plasma fibronectin.
Purified human plasma fibronectin was digested with cathepsin G and the degradation products were tested for reactivity towards a monoclonal antibody. In an immunoblotting assay, after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of the digestion products, the 85 000-Mr and 72 000-Mr gelatin- and heparin-binding fragments as well as the N-terminal 30 000-Mr heparin-binding fragment reacted with the antibody, whereas the 64 000-Mr gelatin- and heparin-binding fragment did not. In enzyme immunoassay the antibody reacted with intact fibronectin and the 30 000-Mr fragment but not with a 40 000-Mr gelatin-binding fragment. The alignment of the binding domains in these fragments and in the intact molecule [Vartio (1982) Eur. J. Biochem. 123, 223-233] localizes the antigenic determinant to the 21 000 Da N-terminal Staphylococcus aureus-binding region of fibronectin.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D056649:Cathepsin G; D002403:Cathepsins; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification; D012697:Serine Endopeptidases; D013917:Thrombin
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005353:Fibronectins; D010446:Peptide Fragments; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D013917:Thrombin