A histological method for the visualization of the intercellular permeability barrier in mammalian stratified squamous epithelia.
Mammalian epidermis and oral epithelial possess an intercellular permeability barrier which is located in the superficial region of the tissue. This study reports a staining reaction which appears to demonstrate a histological correlate of this functional property. Specimens of ear skin, palate, buccal and oesophageal mucosa and of cornea and bladder were obtained from adult rabbits and rats, bisected and either incubated in vitro with 2.5% horseradish peroxidase as a tracer or fixed and processed for light microscopy and stained with a modification of Hart's elastin stain. Examination of specimens prepared by each procedure showed a complementary staining pattern in the intercellular spaces of the stratum corneum or in the superficial region of the non-keratinized tissue. In the epidermis and oral and oesophageal epithelia, the region which excluded the tracer stained with the modified elastin stain. In contrast, the corneal and bladder epithelia neither excluded the tracer nor showed intercellular staining. This relationship between staining of the intercellular space and the exclusion of tracer suggests that the intercellular material in the superficial region of epithelia may be chemically altered to form a barrier substance, possibly as the result of the discharge of the contents of the membrane-coating granules which are present in all the epithelia examined except the cornea and bladder.
D000818:Animals; D004549:Elastin / Q000032:analysis; D004848:Epithelium / Q000033:anatomy & histology* / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D010539:Permeability; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D004549:Elastin; D006735:Horseradish Peroxidase