Use of biotin-labeled monoclonal antibodies and avidin-peroxidase conjugates for the determination of epitope specificities in a solid-phase competitive enzyme immunoassay.
The epitope specificities of monoclonal antibodies against carcinoembryonic antigen (CEA) were determined in a solid-phase, biotin-avidin enzyme immunoassay. Constant amounts of biotin-labeled antibodies were incubated with the immobilized antigen in the presence of decreasing amounts of unlabeled antibodies. The biotinylated antibodies bound to the antigen were then reacted with avidin-peroxidase conjugates. The activity of the bound peroxidase was determined by the use of o-phenylenediamine and hydrogen peroxide.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000502:physiology; D001360:Avidin; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001710:Biotin; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D001360:Avidin; D001710:Biotin