Partial purification of a calcium ionophore from human uremic plasma and normal urine.
A fraction is isolated from human uremic plasma and normal urine using a three step chromatographic separation :gel permeation on Sephadex G15, then chromatography on hydroxyapatite and finally desalting operation on Sephadex G15. The fraction thus separated is ninhydrine positive and uncouples mitochondria principally releasing the resting respiration. Calcium potentiates the uncoupling, while red ruthenium and magnesium inhibit it. These results are in good agreement with a ionophorous activity of the isolated fraction. An hypothetic physiological role of this compound is there being discussed.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D007476:Ionophores / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D008274:Magnesium / Q000378:metabolism; D008930:Mitochondria, Liver / Q000032:analysis; D051381:Rats; D012430:Ruthenium Red / Q000378:metabolism; D014511:Uremia / Q000097:blood*
D007476:Ionophores; D012430:Ruthenium Red; D008274:Magnesium; D002118:Calcium