Activation of insulin-sensitive phosphodiesterase by lectins and insulin-dextran complex in rat fat cells.
Membrane-bound low-Km cAMP phosphodiesterase was activated by concanavalin A, wheat germ agglutinin, and insulin-dextran complex under conditions of incubation with intact rat fat cells. Concanavalin A rapidly stimulated the enzyme activities and maximum was reached at 10 to 15 minutes. As little as 10 micrograms/mL concanavalin A activated the enzyme and a maximal response was obtained at 100 to 300 micrograms/mL, but concanavalin A and wheat germ agglutinin were less potent than insulin. Specific saccharide inhibitors completely abolished activation of the enzyme by lectins, but had no effect on the activation of insulin. Digestion of fat cells with 1 mg/mL trypsin for 15 minutes completely inhibited activation of the enzyme by insulin. However, concanavalin A was less sensitive to trypsinization. The insulin-dextran complex, which did not penetrate the plasma membrane, activated the enzyme and was one tenth as effective as the native insulin. These results suggest that the insulin-like actions of these lectins are provoked through coupling with the carbohydrate moiety on the cell membrane close to insulin receptors.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014909:Wheat Germ Agglutinins
D003911:Dextrans; D007328:Insulin; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D015105:3',5'-Cyclic-AMP Phosphodiesterases