Alteration in lysosome supravital staining as a marker of hydroxyurea-induced cytotoxicity and its modification by radical scavengers in L5178Y cells in culture.
Supravital staining of lysosomal membranes with euchrysine was employed to study hydroxyurea (HU)-induced side toxic effects in L5178Y lymphoblasts in culture. Exposure of cells to 0.1-1.0-10.0 mM HU-induced progressive increase in the proportion of cells without detectable lysosomal fluorescence. This effect preceded the occurrence of non-viable cells, determined by trypan-blue exclusion test. Addition of alpha-tocopherol, acetylsalicylic acid, sodium benzoate or ascorbic acid to the culture medium afforded a concentration-dependent modification of lysosomal response to HU treatment. It is suggested that the fluorescence technique of supravital lysosome staining can be a useful test in studies on the side toxic effects of free radical-forming drugs and their amelioration by radical scavengers.
D000818:Animals; D002478:Cells, Cultured; D005609:Free Radicals; D006918:Hydroxyurea / Q000378:metabolism / Q000633:toxicity*; D007940:Leukemia L5178 / Q000378:metabolism / Q000648:ultrastructure*; D007942:Leukemia, Experimental / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects*; D051379:Mice; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling
D005609:Free Radicals; D006918:Hydroxyurea