Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo.
An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Utilization of the gelatin-agarose and Boyden chamber assays established that: (a) fragments or extracts of corneas treated with several effectors of angiogenesis in vivo acquired the capacity to mobilize the capillary endothelium in vitro; (b) this mobilization was selective for the capillary endothelium; endothelium from aorta and fibroblasts from human skin or rabbit cornea were unresponsive; and (c) among the effectors of angiogenesis utilized alone; i.e., without the intermediary action of the cornea, none were able to mobilize the capillary endothelium in vitro, except for the heparin-copper complex. The data are interpreted to indicate that new formation of capillaries in vivo is the end result of a cascade of events of which heparin and copper are important components.
D000311:Adrenal Glands / Q000098:blood supply; D000818:Animals; D001011:Aorta / Q000502:physiology*; D002196:Capillaries / Q000502:physiology*; D002417:Cattle; D002460:Cell Line; D002465:Cell Movement; D002999:Clone Cells; D003315:Cornea / Q000502:physiology*; D004727:Endothelium / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D005780:Gelatin; D009389:Neovascularization, Pathologic; D012685:Sepharose
D005780:Gelatin; D012685:Sepharose