Construction of cloned libraries from RNA of human fetal tissues.
We have constructed libraries of recombinant DNA plasmids containing sequences complementary to polyadenylated RNA from a variety of human midtrimester fetal tissues. The bacterial colonies containing these plasmids have been grown and replicated on nitrocellulose filters in a manner that facilitates permanent storage, rapid screening, and transportability to other laboratories. We screened a portion of the library for the presence of repetitive sequences and found that approximately 20% of the clones contain repetitive sequences. We have also shown that some clones contain nonrepetitive sequences. Pools of recombinant cDNA-containing plasmids devoid of repetitive sequences have been constructed to permit the chromosomal localization of a variety of actively transcribed sequences. The construction of such large, tissue-specific clone banks should facilitate the direct isolation, mapping, and characterization of normal and abnormal human genes.
D003001:Cloning, Molecular; D004247:DNA; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005333:Fetus; D006801:Humans; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D011247:Pregnancy; D012313:RNA / Q000235:genetics*
D004274:DNA, Recombinant; D011061:Poly A; D012313:RNA; D004247:DNA