title: RNA synthesis and poly(adenosine diphosphoribosyl) synthetase activity in developing mouse testis.
abstract: RNA polymerase I and II and poly(ADP-ribosyl) synthetase activities were determined in isolated nuclei prepared from mouse testes at 1, 3, and 8 weeks after birth. RNA polymerase II and poly(ADP-ribosyl) synthetase activities increased with progression of spermatogenesis and age while RNA polymerase I activity decreased. The observed inverse relationship of RNA polymerase I and II parallels the shift in species of RNA, produced during spermatogenesis. Poly(ADP-ribosyl) synthetase activity increased with age, reaching a peak at 8 weeks. These enzymatic activities in aged mouse testis nuclei were equivalent to that of 8-week-old mice. Germ cells from the adult testis were separated by unit gravity velocity sedimentation. The enriched fractions containing pachytene and round spermatids possessed RNA polymerase and poly(ADP-ribosyl) synthetase activities. The present results suggest that transcriptional events during spermatogenesis may be modulated by changes in the activities of variants of RNA polymerase.
mesh_terms: D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008297:Male; D051379:Mice; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D013091:Spermatogenesis; D013737:Testis / Q000201:enzymology* / Q000254:growth & development
chemical_list: D012313:RNA; D011065:Poly(ADP-ribose) Polymerases; D012321:DNA-Directed RNA Polymerases; D009244:NAD+ Nucleosidase