Analysis of a major rat idiotype associated with Anti-GAT antibodies.
An anti-idiotypic antiserum was raised in a rabbit against a pool of purified F.344 rat anti-GAT antibodies. GAT-13, the idiotype defined by this serum, is present in all F.344 anti-GAT sera from primary and secondary anti-GAT responses. Anti-GAT sera of 13 inbred rat strains, with different RT1 haplotypes and with different heavy- and light-chain allotypes, all express idiotypic determinants cross-reacting with GAT-13. Thus, like in mice anti-GAT antibodies from rats express public idiotypic determinants. The anti-idiotypic serum also recognizes a highly conserved idiotypic specificity present on mouse and guinea-pig anti-GAT antibodies. The mouse, rat and guinea-pig express a similar highly conserved idiotypic specificity after immunization with GAT. All anti-GAT antibodies from the mouse and guinea-pig bear this idiotypic specificity. These results confirm the existence in the anti-GAT response of interspecies cross-reactive idiotypic determinants.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D000939:Epitopes; D006168:Guinea Pigs; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008297:Male; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010455:Peptides; C022204:anti-GAT antibodies, idiotypic