LPS greatly enhances the antibody response to hapten-polysaccharide conjugates, but not to hapten-protein conjugates.
In confirmation of earlier findings, we observed that an injection of bacterial lipopolysaccharide (LPS) into mice caused a considerable increase in the serum concentrations of IgM and IgG (total Ig rose three- to four-fold in 7 days), and a corresponding increase in the concentrations of "natural" anti-(3-iodo-4-hydroxy-5-nitrophenyl) acetyl (NIP) and anti-trinitrophenol (TNP) antibodies. Our main purpose was to determine what effect LPS had on antigen-dependent responses. Hapten conjugates of a polysaccharide and of proteins were used as antigens. Hapten-protein conjugates induced a strong anti-hapten antibody response (up to 1 mg/ml of anti-hapten antibodies on day 7). Hapten-polysaccharide conjugates induced only a meagre increase in anti-hapten antibodies from the pre-immunization level (maximal concentration 65 micrograms/ml on day 7). LPS, when injected with the antigen, greatly enhanced the antibody response to the hapten-polysaccharide conjugates (up to 2.6 mg/ml of anti-hapten antibodies on day 7). It had little effect on antibody responses to hapten-protein conjugates. The combination treatment had the same effect on immunoglobulin concentrations as LPS alone.
D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D005362:Ficoll / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D051379:Mice; D008808:Mice, Inbred CBA; D009578:Nitrobenzenes / Q000276:immunology*; D009592:Nitrohydroxyiodophenylacetate / Q000008:administration & dosage / Q000276:immunology*; D009596:Nitrophenols / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D009578:Nitrobenzenes; D009596:Nitrophenols; D011134:Polysaccharides; D012709:Serum Albumin; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005719:gamma-Globulins; D005362:Ficoll; D009592:Nitrohydroxyiodophenylacetate