Generation of cytotoxic T lymphocytes against modified self in the absence of antigen by interleukin 2-containing preparations.
Spleen cells obtained from normal mice were cultured with interleukin 2 (IL2); no antigen was added. After 4-5 days, these cultures contained effector cells which lysed autologous spleen target cells that were modified with 2,4,6-trinitrobenzene sulfonic acid or fluorescein isothiocyanate. No killing was seen on unmodified spleen target cells. These effector cells were Thy 1+, Lyt 1-, 2+ and were derived from Thy 1+ precursor cells. IL2 preparations induced the generation of such cytotoxic T lymphocytes (CTL) in a dose-dependent manner. IL2-induced CTL were shown to be different from the natural killer (NK) cells augmented by IL2 by virtue of their time of appearance in culture, by cold-target competition, and by different cell-surface markers. These results demonstrate that the IL2 signal may be sufficient for the induction of the differentiation of CTL precursors in the absence of an antigenic signal.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010641:Phenotype; D011817:Rabbits; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013861:Thiocyanates / Q000494:pharmacology; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology
D000941:Antigens; D000939:Epitopes; D005452:Fluoresceins; D007376:Interleukin-2; D013861:Thiocyanates; D014302:Trinitrobenzenesulfonic Acid; D016650:Fluorescein-5-isothiocyanate