Isolation and characterization of horny cell membrane.
The cornified cell membrane and thickened marginal band were obtained from human stratum corneum by a new, faster procedure involving extraction, tripsinization, and density gradient centrifugation. Observations revealed a fine membranous structure with a thickened inner surface of approximately 150 A (marginal band). Each of the membranous samples contained a level of half-cystines markedly higher in amount than that of earlier findings by Matotsy and Matotsy [5]. The samples also contained high amounts of glutamic acid and lysine residues many of which constitute the epsilon-(gamma-glutamyl) lysine cross-linkages of the cell membrane. This study presents the simple isolation procedure and its results highlight the high content of half-cystines in the basic membrane fraction. In addition, the study reveals the importance of S-S cross-linkages during the formation of the cell membrane.
D002462:Cell Membrane; D002469:Cell Separation / Q000379:methods*; D003553:Cystine / Q000032:analysis; D006801:Humans; D007633:Keratins / Q000032:analysis / Q000378:metabolism; D008297:Male; D012867:Skin / Q000032:analysis / Q000648:ultrastructure*
D003553:Cystine; D007633:Keratins