The induction of anti-erythrocyte antibodies in vivo by administration of lipopolysaccharide.
Experiments were designed to determine whether B-cell activation of control mouse strains with lipopolysaccharide (LPS) could induce the same anti-erythrocyte antibody responses observed to occur spontaneously in the NZB strain. When BALB/c and DBA/2 mice were injected intraperitoneally with 100 micrograms of LPS, antibodies to X, HB, and HOL antigens could be detected 2 weeks later at levels comparable to those found spontaneously in NZB mice. Injection of C3H/HeJ mice, nonresponders to LPS, resulted in no detectable anti-erythrocyte antibody responses. When NZB mice were treated with LPS in this way, serum levels of anti-RBC antibodies increased. A measure of the percentage hemolysis induced by sera from these animals in the presence of an exogenous complement source revealed a higher incidence and hemolytic titer in LPS-injected BALB/c and DBA/2 strains than in PBS-injected mice. In addition, injection of LPS induced the appearance of erythrocyte-bound IgM and IgG in BALB/c, DBA/2, and NZB mice. These data suggest that LPS-induced B-cell activation results in the appearance of anti-erythrocyte antibodies.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis*; D001789:Blood Group Antigens / Q000276:immunology*; D003168:Complement Fixation Tests; D004910:Erythrocyte Membrane / Q000276:immunology* / Q000378:metabolism; D006461:Hemolysis; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D013045:Species Specificity; D000511:alpha-Macroglobulins / Q000008:administration & dosage
D000954:Antigens, Surface; D001323:Autoantibodies; D001789:Blood Group Antigens; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D000511:alpha-Macroglobulins; C032306:polyclonal B cell activator