Spontaneous and 5-azacytidine-induced reexpression of ornithine carbamoyl transferase in hepatoma cells.
Rat hepatoma cells that do not synthesize the hepatic enzyme ornithine carbamoyl transferase spontaneously give rise to producing cells at a low frequency. Reexpression of this differentiation trait is strongly increased by 5-azacytidine treatment, suggesting that hypermethylation plays a critical role in the impaired expression of the ornithine carbamoyl transferase gene in hepatoma cells.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000235:genetics; D008745:Methylation; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D051381:Rats
D009954:Ornithine Carbamoyltransferase; D001374:Azacitidine