title: Studies on the exocytosis of cultured mast cells derived from mouse bone marrow.
abstract: Mast cells were obtained from mouse bone marrow cells cultured for 14 days in medium derived from Concanavalin A (Con A) stimulated mouse spleen cells. Upon passive sensitization of the cultured cells with immunoglobulin E (IgE), histamine release from mast cells was approximately 200% above control within 1 min of incubation with anti-IgE. The calcium inonophore A 23187 also evoked a concentration-dependent (10(-8) M to 6 x 10(-7) M) histamine release following a 6 min incubation. Transmission electron microscopy (TEM) demonstrated that the secretory granules of the cultured cells have a peripheral crystalline pattern, like that previously demonstrated for mast cells. Scanning electron microscopy (SEM) illustrated spherical cells with surfaces traversed by many ridge-like folds. Intragranular fusion, following exposure of the IgE-bearing cells to anti-IgE, led to accumulation of the granules into channels which, on study with both transmission and scanning electron microscopy, appeared to be associated with the cell surface.
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005089:Exocytosis; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning
chemical_list: D003470:Culture Media; D003208:Concanavalin A; D007073:Immunoglobulin E; D000001:Calcimycin