Quantitation of protein by alkaline extraction of naphthol blue black-stained polypeptides in sodium dodecyl sulfate-polyacrylamide slab-gels.
A sodium dodecyl sulfate-polyacrylamide slab-gel system was used to study the alkaline extraction of Naphthol Blue Black B-stained polypeptides. A simple, rapid, and reproducible method for quantitative assessment of 1- to 5-micrograms quantities of individual polypeptides in the molecular weight range of 14,000 to 120,000 is described. The working range of the assay may be extended to the 20-micrograms level at the expense of rapidity.
D000580:Amido Black; D001391:Azo Compounds; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010455:Peptides / Q000032:analysis*; D013053:Spectrophotometry; D013194:Staining and Labeling
D001391:Azo Compounds; D010455:Peptides; D000580:Amido Black