Preparation and antigenic property of methyl 3 beta-hydroxy-19-oxo-5-cholen-24-oate 19-(O-carboxymethyl) oxime-bovine serum albumin conjugate.
The preparation and antigenic property of 3 beta-hydroxy-5-cholen-24-oic acid-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-carboxymethyl) oxime bridge at the C-19 position on the steroid nucleus is described. Antibody raised against antigen in the rabbit possessed high titer and specificity to 3 beta-hydroxy-5-cholen-24-oic acid, exhibiting no significant cross-reactions with various bile acids.
D000936:Antigen-Antibody Complex; D002793:Cholic Acids / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006241:Haptens; D007106:Immune Sera; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D009682:Magnetic Resonance Spectroscopy; D012710:Serum Albumin, Bovine; D013055:Spectrophotometry, Infrared
D000936:Antigen-Antibody Complex; D002793:Cholic Acids; D000939:Epitopes; D006241:Haptens; D007106:Immune Sera; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine; C014596:3 beta-hydroxy-delta 5-cholenic acid