Molecular biological characterization of a highly leukaemogenic virus isolated from the mouse. IV. Viral proteins.
The proteins of a highly leukaemogenic murine virus (DMBA-LV) endogenous to the CFW/D mouse have been characterized. This virus does not contain ecotropic, xenotropic or polytropic type C retroviruses capable of replicating in established tissue culture cell lines. Analysis of the viral proteins indicated that proteins characteristic of both type C and type B retroviruses were present. All the proteins characteristic of a competent, mature type B retrovirus, such as the murine mammary tumour virus, were present in the virions and were expressed at high levels in a virus-producing thymic lymphoma cell line. Type C internal structural proteins were detected in the virus and cells producing virus. Type C envelope protein was detected at very low levels in both the virions and the virus-producing tumour cell lines. Chymotryptic polypeptide profiles of this gp70 indicated similarity with the envelope glycoproteins characteristic of xenotropic and recombinant type C retroviruses.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D008223:Lymphoma / Q000139:chemically induced; D051379:Mice; D011863:Radioimmunoassay; D012190:Retroviridae / Q000032:analysis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene