5-Azacytidine increases the synthesis of embryonic hemoglobin (E2) in murine erythroleukemic cells.
The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-azacytidine treatment. The level of hemoglobin synthesis in DMSO-induced cells is less than BA-induced cells while the effect of the 5-azacytidine stimulation was greater with DMSO induction than with BA induction.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D005622:Friend murine leukemia virus; D005786:Gene Expression Regulation; D006454:Hemoglobins / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D014176:Protein Biosynthesis
D002087:Butyrates; D006454:Hemoglobins; D020148:Butyric Acid; D005319:Fetal Hemoglobin; D001374:Azacitidine; D004121:Dimethyl Sulfoxide