In vitro biosynthesis of mouse hair keratins under the direction of follicular RNA.
A hair follicle-enriched fraction from neonatal C57BL/6J mouse skin has been obtained by a newly-developed preparative procedure. RNA isolated from this source directs protein synthesis in a cell-free translation system derived from rabbit reticulocytes. Translation products in the molecular weight range of keratins (45,000-70,000 Mr) are encoded by RNA species which sediment at 18S on sucrose density gradients. Proteins of 63K, 59K, 58K, 47K and 46K Mr were identified as keratins on the basis of electrophoretic mobilities identical with authentic hair keratins and by immunoprecipitation with keratin antiserum.
D000818:Animals; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005815:Genetic Code; D006197:Hair / Q000276:immunology / Q000378:metabolism*; D066298:In Vitro Techniques; D007633:Keratins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification*; D011817:Rabbits; D014508:Urea / Q000008:administration & dosage
D012333:RNA, Messenger; D007633:Keratins; D014508:Urea