Characterization of N-glycolyneuraminic acid-containing glycosphingolipids from a Marek's disease lymphoma-derived chicken cell line, MSB1, as tumor-associated heterophile Hanganutziu-Deicher antigens.
Heterophile, Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acid-containing glycosphingolipids (GSLs) were detected as tumor-associated foreign antigens of a Marek's disease lymphoma-derived cell line, MSB1, by enzyme-immunoassay with chicken antibody against N-glycolylneuraminyl-lactosylceramide (anti-NeuGc-LacCer). At least three species of HD antigen-active GSLs were detected by two-dimensional thin-layer chromatography (TLC) combined with enzyme-immunoassay. The reactivities of the GSLs with anti-NeuGc-LacCer, their behaviors on two-dimensional TLC and the results of an endo-beta-galactosidase digestion study indicated that these three GSLs were NeuGc-LacCer (NeuGc alpha 2-2Gal beta 1-4Glc-Cer), NeuGc-nLcOse4Cer (NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer) and NeuGc-nLcOse6Cer (NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer).
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D002460:Cell Line; D002645:Chickens / Q000276:immunology*; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D006026:Glycoside Hydrolases; D006028:Glycosphingolipids / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D008380:Marek Disease / Q000276:immunology*; D009438:Neuraminic Acids / Q000302:isolation & purification; D013194:Staining and Labeling; D001616:beta-Galactosidase
D000951:Antigens, Neoplasm; D006028:Glycosphingolipids; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase