Comparison of monoclonal and polyclonal antibodies in a two-site binding enzyme immunoassay for alphafetoprotein (AFP).
A two-site binding enzyme immunoassay for the detection of alphafetoprotein (AFP) was developed by using either a combination of two monoclonal antibodies or of one monoclonal antibody and polyclonal antibodies. The conjugation of the monoclonal antibodies to peroxidase by the periodate method yielded a somewhat higher sensitivity in the enzyme immunoassay (EIA), when compared to conjugates produced by the glutaraldehyde method. The detection limit was 10 micrograms AFP per litre when using only monoclonal antibodies in the assay. Simultaneous incubation of the sample and the monoclonal labelled antibody should be avoided unless using at least two different dilutions of the sample for investigation.
D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D005976:Glutaral; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D051379:Mice / Q000276:immunology; D008832:Microchemistry; D010504:Periodic Acid; D012756:Sheep / Q000276:immunology; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D000509:alpha-Fetoproteins; D010504:Periodic Acid; C009288:metaperiodate; D005976:Glutaral