Synthesis of full-length cDNAs from partially purified human procollagen mRNAs.
Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis* / Q000302:isolation & purification; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics; D011132:Polyribosomes / Q000378:metabolism; D011247:Pregnancy; D011347:Procollagen / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D011347:Procollagen; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase