Suppression by serum of immunoreactivity to a common tumour antigen in cancer patients.
Leucocytes from 4 cancer patients showed cellular reactivity in the leucocyte adherence inhibition (LAI) assay in the presence of the synthetic encephalitogenic peptide of human myelin basic protein. All patients exhibited reactivity at a peptide concentration of 500 ng/ml. Leucocytes from 4 non-cancer patients failed to react. Suppression of LAI was detected in all 4 cancer patients by adding their serum to reactive mixtures containing peptide and autologous leucocytes. Each serum was subjected to column chromatography on Sephacryl S-200 to determine the molecular weight distribution of suppressive (blocking) factors. The greatest suppression was found in all cases within the range 90-155 kdalton.
D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D007414:Intestinal Neoplasms / Q000276:immunology; D007957:Leukocyte Adherence Inhibition Test; D008175:Lung Neoplasms / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008875:Middle Aged; D004676:Myelin Basic Protein / Q000494:pharmacology*; D009369:Neoplasms / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology
D000951:Antigens, Neoplasm; D004676:Myelin Basic Protein; C020163:blocking factor