Characterization of an antigen-specific suppressive factor derived from a cloned suppressor effector T cell line.
A cloned effector-type suppressor T cell line, 3D10, which is known to suppress the antibody response against dinitrophenylated keyhole limpet hemocyanin (KLH), produced a soluble KLH-specific factor (TsF) that can replace the function of parental T cell clones. High activity of TsF was released spontaneously into the culture supernatant when cultured in interleukin 2 (IL 2)-containing medium, requiring no antigenic stimulation. The culture supernatant of 3D10 was also capable of inhibiting the KLH-induced proliferative response of primed T cells in an antigen-specific manner. The direct target of TsF was found to be Lyt-1+2- T cells undergoing an early stage of antigen-specific proliferation. TsF was antigen binding but lacked any other serologic markers such as I-J and immunoglobulin heavy chain-linked allotypic determinants on T cells. No genetic restriction was found in its action on allogeneic T cells. The production of IL 2 in proliferative T cells by antigenic stimulation was not inhibited by TsF. These results indicate that the TsF described here is the legitimate mediator produced by the effector-type suppressor T cell that suppresses the antigen-specific responses of Lyt-1+2- T cells. The m.w. of TsF was approximately 75,000.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics; D006433:Hemocyanins / Q000276:immunology; D007164:Immunosorbents / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000950:Antigens, Ly; D000939:Epitopes; D006183:H-2 Antigens; D007164:Immunosorbents; D007376:Interleukin-2; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin