A trans-acting regulatory product necessary for expression of the Drosophila melanogaster 68C glue gene cluster.
The mutation l(1)npr-1 is located at cytological location 2B5 on the X chromosome in Drosophila melanogaster. We have found that this mutation causes absence of the normal product of the 2B5 locus and that it has the following phenotypes: the 68C glue puff on the third chromosome does not regress when mutant salivary glands are cultured in the presence of ecdysterone; the three 68C glue protein mRNAs are not synthesized; and a transformed Drosophila strain carrying both a normal resident 68C Sgs-3 gene and an introduced functional Sgs-3 gene with only a few kb of flanking sequences expresses neither Sgs-3 RNA if the l(1)npr-1 mutation is crossed into the stock. Thus the normal product of the l(1)npr-1 gene is required for regression of the 68C puff, and the l(1)npr-1 gene product allows expression of the Sgs-3 gene by interacting, either directly or indirectly, with DNA sequences near this glue protein gene.
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000502:physiology; D004331:Drosophila melanogaster / Q000235:genetics*; D004441:Ecdysterone / Q000494:pharmacology; D005786:Gene Expression Regulation; D005796:Genes; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D012469:Salivary Glands / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000235:genetics*
D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; D004441:Ecdysterone; D012313:RNA