Analysis of idiotypic heterogeneity in the anti-alpha 1-3 dextran and anti-phosphorylcholine responses using monoclonal anti-idiotype antibodies.
Panels of monoclonal anti-idiotype antibodies (MAIDs) specific for individual (IdI) and cross-reactive (IdX) idiotopes were prepared and used to study the expression of these idiotopes on anti-DEX and anti-PC antibodies produced in response to antigenic stimulation in vivo, clonal expression of idiotopes in an in vitro splenic focus assay, and the alterations in the idiotypic profile of these responses after in vivo administration of monoclonal anti-Id antibodies. Using these panels of MAIDs, it was possible to inactivate IdI-bearing B cells both in neonates and adult mice without affecting the responsiveness of IdI- B cells. By contrast, suppression with IdX-specific antibodies resulted in greatly reduced antibody responses. By studying the idiotypic profile of anti-DEX clones in the splenic focus assay, it was shown that IgM, IgG, and IgA antibody Id were diverse and paralleled those expressed in serum. Within some clones there was evidence that idiotope-isotype associations differed, suggesting that V region variants may have been generated within the progeny of a clone following stimulation by dextran. An anti-anti-Id antibody isolated from a BALB/c mouse undergoing a normal immune response to R36A was shown to have a T-cell independent highly idiotope-specific regulatory effect on the T15+ anti-PC response, apparently affecting induction of anti-idiotypic B cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002794:Choline / Q000031:analogs & derivatives*; D003911:Dextrans / Q000276:immunology*; D005786:Gene Expression Regulation; D006241:Haptens; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D010641:Phenotype; D010767:Phosphorylcholine / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D003911:Dextrans; D006241:Haptens; D007130:Immunoglobulin Idiotypes; C040834:alpha-1,3-dextran; D010767:Phosphorylcholine; D002794:Choline