Simultaneous localization of two different tissue antigens based on the silver intensified PAP-DAB and on the traditional PAP-DAB method.
A new immunohistological method was elaborated for the light-microscopic detection of two tissue antigens in the same section. One of the antigens was visualized by the silver-gold intensified peroxidase-antiperoxidase complex (PAP)-diaminobenzidine (DAB) method resulting in a black coloured end-product. The other antigen was labelled by the brown reaction-product of the traditional PAP-DAB method. Both reaction-products are stable, electron-dense and they can clearly be distinguished from each other.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis; D007124:Immunoenzyme Techniques; D008473:Median Eminence / Q000032:analysis / Q000276:immunology*; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000276:immunology*; D051381:Rats; D012834:Silver; D013004:Somatostatin / Q000032:analysis; D013194:Staining and Labeling
D000941:Antigens; D007987:Gonadotropin-Releasing Hormone; D012834:Silver; D013004:Somatostatin; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone