Phenotypic characterization of cells within subcutaneous rheumatoid nodules.
An immunohistochemical double-staining technique was applied to frozen sections of biopsies from subcutaneous rheumatoid nodules. Material within the central necrosis reacted with anti-immunoglobulin and anti-HLA-DR antibodies. Cells within the palisading layer were "macrophage-like," as indicated by staining for anti-HLA-DR and OKM1, and showed a high proliferative activity as seen by OKT9 reactivity. The granulation zone contained many (alpha Leu-1+) T cells in close contact with HLA-DR-expressing non-T cells.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001706:Biopsy; D005260:Female; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006651:Histocytochemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007158:Immunologic Techniques; D008297:Male; D009336:Necrosis; D010641:Phenotype; D012218:Rheumatoid Nodule / Q000473:pathology*; D012867:Skin; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera