In vitro synthesis of type IV procollagen.
Total RNA was isolated from parietal endoderm cells of 131/2-day mouse embryos that synthesize large amounts of type IV procollagen. In vitro translation of this RNA in the reticulocyte lysate supplemented with a ribonuclease inhibitor yielded two equally prominent polypeptides of Mr = 165,000 and 168,000, immunoprecipitable with anti-mouse type IV collagen serum. The Mr = 165,000 polypeptide was shown by one-dimensional peptide mapping to represent an unmodified chain of type IV procollagen. The Mr = 168,000 polypeptide, the in vitro synthesis of which was barely detectable in the absence of a ribonuclease inhibitor, most likely represents the other genetically distinct chain of type IV procollagen. Similar results to those described were also obtained using poly(A) + RNA prepared from murine F9 embryonal carcinoma cells induced to differentiate in vitro into parietal endoderm.
D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000302:isolation & purification; D004622:Embryo, Mammalian; D004707:Endoderm / Q000378:metabolism; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D011347:Procollagen / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D013724:Teratoma
D046911:Macromolecular Substances; D011347:Procollagen; D012313:RNA; D003094:Collagen