Preparation and properties of monomercurated tRNA.
Monomercurated tRNA has been prepared by exhaustive treatment of mercaptoethanol on polymercurated tRNA under non-denaturing conditions. The remained mercury atom is stable toward ultraviolet (254 nm) irradiation up to absorption of 100 quanta/nucleotide, but could be easily removed by mercaptoethanol under denaturing conditions. Monomercurated tRNA quantitatively binds to thiopropyl-Sepharose and can be completely desorbed by mercaptoethanol-containing solutions. Monomercurated tRNA is similar to the starting tRNA preparation in efficiency and specificity of aminoacylation and in factor-dependent polypeptide synthesis in vitro.
D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D004926:Escherichia coli; D009941:Organomercury Compounds / Q000138:chemical synthesis*; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012346:RNA, Transfer, Amino Acyl
D009941:Organomercury Compounds; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer