Expression of canine parvovirus-beta-galactosidase fusion proteins in Escherichia coli.
Cloned DNA fragments encoding portions of canine parvovirus (CPV) structural proteins were inserted into plasmid expression vectors. These plasmids expressed CPV-beta-galactosidase fusion proteins under the transcriptional control of the Escherichia coli lac promoter-operator. The fusion proteins were purified and used to immunize rabbits. Rabbit antibodies raised against these fusion proteins were shown to immunoprecipitate authentic CPV structural proteins from infected cell extracts. This demonstrated that the CPV-beta-galactosidase fusion proteins expressed in bacteria elicit antibodies which can recognize determinants of authentic CPV proteins. However, none of the antibodies neutralizes CPV virus particles.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D002213:Capsid / Q000235:genetics / Q000276:immunology; D004274:DNA, Recombinant; D004285:Dogs; D000939:Epitopes; D004926:Escherichia coli / Q000378:metabolism; D005786:Gene Expression Regulation; D008970:Molecular Weight; D010321:Parvoviridae / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D000956:Antigens, Viral; D004274:DNA, Recombinant; D000939:Epitopes; D014764:Viral Proteins; D001616:beta-Galactosidase