Melittin-stimulated arachidonic acid metabolism by cultured malignant human epidermal keratinocytes.
Upon melittin stimulation, cultured SCC-13 keratinocytes release prostaglandins E2, F2 alpha, 6-keto-F1 alpha, thromboxane B2, leukotriene B4, and 6-sulfido-peptide-containing leukotrienes (SRS) into serum free medium. Release of prostaglandins E2, F2 alpha, and SRS, normalized to cell protein, is 3- to 10-fold higher from rapidly growing than confluent cultures. Cells growing with hydrocortisone in the medium produce approximately twice the level of the cyclooxygenase-mediated metabolites PGE2 and PGF2 alpha as those without hydrocortisone, but similar levels of the lipoxygenase-mediated metabolite SRS. The results demonstrate the potential utility of squamous carcinoma lines for investigating biochemical pathways of arachidonic acid metabolism in keratinocytes.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001514:Bee Venoms / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D007633:Keratins; D007700:Kinetics; D008555:Melitten / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis*; D013189:SRS-A / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000378:metabolism*
D001095:Arachidonic Acids; D001514:Bee Venoms; D011453:Prostaglandins; D013189:SRS-A; D008555:Melitten; D016718:Arachidonic Acid; D007633:Keratins