Estrogen receptors in carcinoma of the breast. A comparison of the dextran-coated charcoal, immunofluorescent, and immunoperoxidase technics.
This study compares the estrogen receptor (ER) content of 26 breast neoplasms as determined by dextran-coated charcoal (DCC), fluorescein-labeled estradiol conjugate (FITC) on frozen sections, and immunoperoxidase (IP) on sections of formalin-fixed, paraffin-embedded tissues. Concordance between results of the three technics was found in 16 (62%) cases. DCC and IP agreed in 18 (69%) cases, DCC and FITC agreed in 22 (85%) cases, and IP agreed with FITC in 18 (69%) cases. Most of the concordant cases (15/16) were in patients with ER-positive neoplasms. The relative merits and applications of the three technics are discussed.
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology; D002606:Charcoal; D003911:Dextrans; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D011446:Prospective Studies; D011960:Receptors, Estrogen / Q000302:isolation & purification*; D013194:Staining and Labeling; D013861:Thiocyanates
D003911:Dextrans; D005452:Fluoresceins; D011960:Receptors, Estrogen; D013861:Thiocyanates; D002606:Charcoal; D016650:Fluorescein-5-isothiocyanate