Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor.
A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.
D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification*; D000818:Animals; D002196:Capillaries / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002642:Chick Embryo; D002813:Chondrosarcoma / Q000032:analysis*; D002846:Chromatography, Affinity; D004247:DNA / Q000096:biosynthesis; D004727:Endothelium / Q000166:cytology; D006133:Growth Substances / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism*; D051379:Mice; D009389:Neovascularization, Pathologic
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D006493:Heparin; D004247:DNA