Generation of an internal matrix in mature avian erythrocyte nuclei during reactivation in cytoplasts.
When fused with mouse L-cell cytoplasts, chick erythrocyte nuclei enlarge, take up proteins from the host cytoplasm, and recommence RNA synthesis. We found that during this transition the erythrocyte nuclei gain an internal nuclear matrix, thus providing a novel approach to questions concerning the nature of the salt-resistant intranuclear skeleton. A new method for preparation and examination of the nuclear matrix in situ is also described.
D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus / Q000378:metabolism*; D002642:Chick Embryo; D002843:Chromatin / Q000378:metabolism; D004261:DNA Replication; D004912:Erythrocytes / Q000648:ultrastructure*; D051379:Mice; D012313:RNA / Q000096:biosynthesis; D013997:Time Factors
D002843:Chromatin; D012313:RNA