A new technique for perfusion fixation and contrast enhancement of foetal lamb myocardium for electron microscopy.
A technique of perfusion fixation following elective diastolic cardiac arrest was developed to obtain excellent preservation of foetal lamb hearts for morphological studies with electron microscopy. A technique was developed to markedly enhance the contrast of embedded material by treating thin sections with tannic acid prior to lead staining. This technique has been particularly useful for quantitative morphometric analysis.
D000818:Animals; D002951:Citrates; D019343:Citric Acid; D005404:Fixatives; D006321:Heart / Q000196:embryology*; D006652:Histological Techniques; D047348:Hydrolyzable Tannins; D008854:Microscopy, Electron / Q000379:methods*; D009206:Myocardium / Q000648:ultrastructure*; D009942:Organometallic Compounds; D010477:Perfusion; D012756:Sheep / Q000196:embryology*; D013194:Staining and Labeling; D014501:Uranium
D002951:Citrates; D005404:Fixatives; D047348:Hydrolyzable Tannins; D009942:Organometallic Compounds; C005460:uranyl acetate; D019343:Citric Acid; D014501:Uranium