Purification and characterization of beta-galactoside-binding lectin from chick embryonic skin.
Lectin activity was found in tarsometatarsal skin of chick embryo. It was specific for beta-linked galactosyl residues and required a thiol-reducing agent for hemagglutination activity. The lectin was extracted from dermis and epidermis (skin) with lactose and purified to apparent homogeneity by affinity chromatography on asialofetuin-Sepharose. Examination of their biochemical properties showed that although dermis and epidermis develop from different origins, they contain the same lectin. The apparent subunit Mr of lectin was 14000 and its isoelectric point was 7.0. Under non-dissociating conditions, the lectin exists mainly as a dimer. Radioimmunoassay showed that this skin-type lectin is present in many tissues including skin, muscle, bone, eye, heart, liver and brain at various developmental stages. A wide distribution and a marked change in its content during development strongly suggest that the lectin might have a fundamental role in cellular function, embryonic development and tissue differentiation.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001212:Asialoglycoproteins; D002642:Chick Embryo; D002846:Chromatography, Affinity; D004589:Electrophoresis, Disc; D060748:Fetuins; D005697:Galactosides; D037161:Galectins; D006384:Hemagglutination; D037102:Lectins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011863:Radioimmunoassay; D012867:Skin / Q000196:embryology / Q000276:immunology*; D000509:alpha-Fetoproteins
D000596:Amino Acids; D001212:Asialoglycoproteins; D060748:Fetuins; D005697:Galactosides; D037161:Galectins; D037102:Lectins; D046911:Macromolecular Substances; D000509:alpha-Fetoproteins; C017029:asialofetuin; C007816:beta-galactoside