Structural and genetic analyses of HLA class I molecules using monoclonal xenoantibodies.
The monoclonal antibodies B1.23.2 and B9.12 were developed to characterize human class I major histocompatibility gene products. They detect two different epitopes and define on a given lymphoblastoid B cell line two subsets of beta 2 microglobulin-complexed HLA class I molecules. One subset reacts with B9.12 and B1.23.2 and the other one expresses only the B9.12 epitope. Binding assays were performed on C3H mouse L cells which had been transformed with various single HLA-class I genes and the two detected molecular subsets were shown to be encoded by different genetic loci. Unlike B1.23.2, B9.12 detects all the beta 2m-complexed molecules expressed in human B cell lines and recognizes an epitope different from the one defined by W6/32. In contrast to the vast majority of the other reported anti-class I monoclonal antibodies (including B9.12), B1.23.2 recognizes an epitope expressed on both beta 2m-associated and -free HLA class I heavy chains.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes; D006680:HLA Antigens / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D013045:Species Specificity; D013329:Structure-Activity Relationship; D001613:beta 2-Microglobulin / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D046911:Macromolecular Substances; D001613:beta 2-Microglobulin