title: Comparison of proteins in CSF of lateral and IVth ventricles during early development of fetal sheep.
abstract: This study examines the relationship between plasma proteins in blood and in CSF in the developing brain of sheep fetuses between 30 and 60 days gestation. Five proteins account for the very high concentration of protein in fetal CSF (over 1000 mg/100 mg/100 ml at 30 days): alpha-feto-protein, fetuin, albumin, alpha 1-antitrypsin and transferrin; the concentration of each protein is similar in lateral and IVth ventricular CSF at 30 days. By 40 days there is considerable decrease in protein concentration in lateral ventricular CSF. At this age in the IVth ventricle the overall total was unchanged, although there were changes in concentration of individual proteins. At 60 days the concentration of each protein in both compartments had fallen below that at 40 days; the marked concentration difference between lateral and IVth ventricular CSF was still present. Experiments using i.v. [125I]- or [3H] labeled plasma proteins in 30-40-day fetuses showed that very little protein penetrated into lateral ventricular CSF by 3-5 h after injection; in the same experiments [125I]albumin reached a CSF/plasma ratio of about 15% in the IVth ventricle (compared with 55% for the natural steady state). Autoradiographic studies carried out on material from the same animals did not give evidence for transfer of labeled protein across the choroid plexuses although any such penetration may have been below the threshold of the method. Other explanations for the high concentration of protein in CSF that were considered include penetration via cerebral vessels and synthesis of plasma proteins by choroid plexus epithelial cells or neurons within the brain.
mesh_terms: D000418:Albumins / Q000032:analysis; D000818:Animals; D001812:Blood-Brain Barrier; D002552:Cerebral Ventricles; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D012756:Sheep / Q000196:embryology*; D014168:Transferrin / Q000032:analysis; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D000418:Albumins; D002556:Cerebrospinal Fluid Proteins; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins