Characterization of an epithelioid cell line derived from rat small intestine: demonstration of cytokeratin filaments.
A cell line (RIE-1) of epithelioid morphology was established from rat small intestine. RIE-1 cells possess the normal rat diploid number of chromosomes (42) up to at least passage 20. Both early (less than 10) and late (greater than 20) passage cells are non-tumourigenic and do not form colonies in soft agarose. RIE-1 cells bind two epithelial-specific monoclonal antibodies. In addition, the cells exhibit a network of cytoskeletal filaments which are labelled by an antiserum to keratin. These findings provide strong evidence for an epithelial origin of the RIE-1 cell line.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003599:Cytoskeleton / Q000648:ultrastructure*; D004171:Diploidy; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D007421:Intestine, Small / Q000166:cytology / Q000648:ultrastructure*; D007633:Keratins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D007633:Keratins