Suppression of rabbit lymphocyte functions by antibodies specific for allotypic membrane determinants.
Regulation of immunoglobulin synthesis and secretion was analyzed by exposing spleen cells of b4b4 rabbits to anti-b4 for 24 hr in culture. As noted previously, no lymphocytes with membrane-bound b4 were found immediately after pulse treatment, but substantial regeneration of membrane Ig (mIg) occurred on further culture in antibody-free medium. Splenocytes cultured either in the presence or absence of anti-b4 showed a marked loss of Ig-secreting cells (ISC) after 24 hr in culture but recovered and exhibited peak numbers of ISC on Day 2. However, ISC formation in cultures of antibody-treated cells was significantly suppressed and thereafter declined at a more rapid rate than in control cultures. Polyclonal B cell activators from Nocardia and from gram-negative bacteria stimulated ISC formation in cultures of normal spleen cells, but responsiveness to these activators was depressed following antibody treatment. Antibody-induced suppression of Ig synthesis was attributed to interference with differentiation of B lymphocytes to the secretory stage.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains; D007136:Immunoglobulins / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology*; D011817:Rabbits; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007145:Immunoglobulin kappa-Chains; D007136:Immunoglobulins; C496591:kappa allotype b4, rabbits