Activation of human ribosomal genes by 5-azacytidine.
Cultured human fibroblasts were exposed to 5-azacytidine which inhibits methylation of newly synthesized DNA. A significant increase in the mean number of Ag-stained active nucleolus organizers has been observed in treated cells. This suggests that DNA methylation is involved in modulation of human rRNA synthesis.
D001374:Azacitidine / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008745:Methylation; D009697:Nucleolus Organizer Region / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000235:genetics*
D012335:RNA, Ribosomal; D004247:DNA; D001374:Azacitidine