Indirect evidence of cell wall alterations in Pseudomonas aeruginosa by immunoglobulin preparations.
Ten strains of Pseudomonas aeruginosa isolated from different clinical sources were incubated with immunoglobulin preparations for i.v. use. This resulted in the release of beta-lactamases normally cell-bound in the periplasmic space. The amount of released beta-lactamases - depending on the degree of cell wall alteration - was spectrophotometrically determined by the use of nitrocefin as an indicator substance. beta-lactamase release varies according to strain specificity and depends on the chemical modification of the immunoglobulins used.
D002473:Cell Wall / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000031:analogs & derivatives* / Q000494:pharmacology*; D016756:Immunoglobulins, Intravenous; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000648:ultrastructure*; D001618:beta-Lactamases / Q000378:metabolism; D005719:gamma-Globulins / Q000494:pharmacology*
D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D005719:gamma-Globulins; C031151:pepsinated immunoglobulin G; D001618:beta-Lactamases