Correlation between high-performance liquid chromatography and automated fluorimetric methods for the determination of dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid in nervous tissue and cerebrospinal fluid.
The correlation between automated fluorimetric methods and high-performance liquid chromatography is described for the determination of homovanillic acid and 5-hydroxyindoleacetic acid in cerebrospinal fluid, and for dopamine, 3,4-dihydroxyphenylacetic acid and homovanillic acid in striata of rat brain. The automated fluorimetric methods for 3,4-dihydroxyphenylacetic acid and homovanillic acid showed a good correlation with the high-performance liquid chromatographic methodology. The fluorimetric determination for dopamine was somewhat less reliable than the high-performance liquid chromatographic assay. The fluorimetric assay for 5-hydroxyindoleacetic acid correlated poorly with the chromatographic method.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis* / Q000134:cerebrospinal fluid; D004563:Electrochemistry; D005260:Female; D005470:Fluorometry; D006719:Homovanillic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D006897:Hydroxyindoleacetic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid