title: A rapid method for the inactivation of virus infectivity prior to assay for interferons.
abstract: Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of alpha, beta or gamma mouse interferons.
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D007372:Interferons / Q000032:analysis*; D007783:Lactones / Q000494:pharmacology*; D051379:Mice; D011420:Propiolactone / Q000494:pharmacology*; D012672:Semliki forest virus / Q000187:drug effects / Q000472:pathogenicity; D014774:Virulence / Q000187:drug effects; D014780:Viruses / Q000187:drug effects* / Q000472:pathogenicity
chemical_list: D007783:Lactones; D011420:Propiolactone; D007372:Interferons