Targeting of asialofetuin sugar chain-bearing liposomes to liver lysosomes.
Specific direction of liposomes bearing an asialofetuin sugar chain (AFSC) to liver parenchymal cells was examined both in vivo and in vitro. The AFSC-bearing liposomes were preferentially recovered in the liver within several minutes after an intravenous injection into mice and were found to be predominantly localized in mitochondrial-lysosomal fraction. The massive distribution of the AFSC-liposomes in this fraction was also confirmed by using a lysosomal protease inhibitor, E-64-d. In isolated rat hepatocytes, the uptake of AFSC-liposomes was increased 2-3-fold as compared with the control liposomes without AFSC. Thus liposomes bearing AFSC would be useful to target enzymes to liver lysosomes.
D000818:Animals; D001212:Asialoglycoproteins; D002401:Cathepsin B; D002403:Cathepsins / Q000378:metabolism; D060748:Fetuins; D007700:Kinetics; D008081:Liposomes / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D051379:Mice; D009844:Oligosaccharides / Q000378:metabolism*; D009928:Organ Specificity; D014280:Triglycerides / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism*
D001212:Asialoglycoproteins; D060748:Fetuins; D008081:Liposomes; D009844:Oligosaccharides; D014280:Triglycerides; D000509:alpha-Fetoproteins; C017029:asialofetuin; C005859:tripalmitin; D002403:Cathepsins; D002401:Cathepsin B