Elimination of mycoplasma contamination from mammalian cell cultures by the bibenzimidazole derivative Hoechst 33258.
Cell cultures contaminated with mycoplasma or bacterial L-forms were treated with 8 micrograms/ml of Hoechst 33258. No microorganisms could be detected by fluorescence microscopy after two rounds of treatment.
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D006690:Bisbenzimidazole / Q000494:pharmacology*; D002478:Cells, Cultured / Q000382:microbiology*; D006224:Cricetinae; D006801:Humans; D007740:L Forms / Q000187:drug effects / Q000254:growth & development; D051379:Mice; D008856:Microscopy, Fluorescence; D009174:Mycoplasma / Q000187:drug effects / Q000254:growth & development*
D001562:Benzimidazoles; D006690:Bisbenzimidazole