Proliferative response of human venous endothelial cells to medium conditioned by human tumour cells.
Serum-free medium conditioned by the prior cultivation of cells of the IPT line (derived from a poorly differentiated carcinoma of the lung) contained material of a high MW which stimulated proliferation in cultures of endothelial cells from human umbilical vein. A response was shown by cells seeded on plastic, gelatin or collagen of type I or type IV, at densities of 25 to 200/mm2, and in media with serum concentrations ranging from 0.5 to 20% v/v. Cells plated on biological substrata showed a greater response than those on plastic.
D043925:Angiogenesis Inducing Agents / Q000378:metabolism / Q000494:pharmacology*; D002277:Carcinoma / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology* / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
D043925:Angiogenesis Inducing Agents; D003470:Culture Media; D006133:Growth Substances