Cloning of African trypanosomes in mice immunosuppressed by cyclophosphamide treatment.
The use of cyclophosphamide-treated mice for purpose of cloning the African trypanosomes was assessed. C3HeB/FeJ mice were injected with 200 mg/kg cyclophosphamide (CY) 24-72 hours prior to infection with a single trypanosome isolated from Trypanosoma rhodesiense infected blood samples. All CY-treated mice exhibited depressed parasite-exposure, which was a period of time sufficient to grow trypanosomes from a single organism to a fulminating parasitemia. Cloning efficiency was routinely 45% in these animals. Thus, our study demonstrates that CT-treated mice are a convenient and efficient vehicle for cloning African trypanosomes. Techniques which facilitate the selection of single trypanosomes from infected blood are also described in this report.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002999:Clone Cells / Q000469:parasitology; D003520:Cyclophosphamide / Q000494:pharmacology*; D000939:Epitopes; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014345:Trypanosoma / Q000276:immunology / Q000302:isolation & purification*; D014353:Trypanosomiasis, African / Q000276:immunology / Q000469:parasitology*
D000939:Epitopes; D003520:Cyclophosphamide