Modulation of the effect of histamine-releasing lymphokine on human basophils.
Agents which increase or mimic intracellular cyclic 3',3'-adenosine monophosphate (cAMP) - theophylline dibutyryl cAMP (DBcAMP) and isoprenaline (in the presence of theophylline) - all produced pronounced inhibition of histamine release from human basophils, thus suggesting a regulatory role for the cAMP system. The effect of the flavonoids , quercetin and taxifolin , and the structurally-related cromone disodium cromoglycate (DSCG) was also studied. Only quercetin was effective in inhibiting histamine release. This is similar to the situation in IgE-mediated release. The microtubule stabilizer, deuterium oxide (D2O), at a concentration of 44% caused up to three-fold increase in release. This supports the belief that histamine release by this histamine-releasing factor ( HRF ) is a secretory process. Indomethacin and 5,8,11,14-eicosatetraynoic acid (ETYA), which are modulators of arachidonic acid metabolism, produced little or no inhibition of histamine release by HRF , thus suggesting that the release is largely independent of the arachidonate system, probably unlike IgE-mediated release.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001491:Basophils / Q000187:drug effects*; D003994:Bucladesine / Q000494:pharmacology; D003903:Deuterium / Q000494:pharmacology; D017666:Deuterium Oxide; D005419:Flavonoids / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology; D014867:Water / Q000494:pharmacology
D001095:Arachidonic Acids; D005419:Flavonoids; D008222:Lymphokines; D014867:Water; D015117:5,8,11,14-Eicosatetraynoic Acid; D016718:Arachidonic Acid; D003994:Bucladesine; D003903:Deuterium; D013806:Theophylline; D017666:Deuterium Oxide