Antigen-presenting capacity of guinea pig B lymphocytes in T cell proliferative response in vitro.
The capacity of normal (unprimed) B cells and keyhole-limpet-hemocyanin(KLH)-primed B cells to present the antigen KLH to KLH-primed T cells in the guinea pig was examined with in vitro assay of lymphocyte proliferative response. Antigen-pulsed B-lymphocyte population, extensively devoid of macrophages (M phi), induced the proliferative response of KLH-primed T lymphocytes, although less effectively than the peritoneal M phi. The antigen presentation was antigen-specific. There was no substantial difference between the magnitude of T-cell response induced by KLH-primed B-cell population and that stimulated by unprimed, normal B-cell population. The antigen-presenting capacity of the B-cell population was abrogated by pretreatment with anti-guinea pig immunoglobulin (Ig) or anti-I-region-associated (Ia) antigen antiserum and complement, whereas it was not affected with anti-guinea pig M phi antiserum and complement. From studies using strain 2 and strain 13 guinea pigs, histocompatibility requirement between antigen-pulsed B cells and antigen-reactive T cells was suggested: B lymphocytes, as well as M phi, did elicit proliferative response to specific antigen, KLH or purified protein derivative of tuberculin (PPD), in syngeneic, but not in allogeneic, T cells. These results suggest that the Ia-positive, surface-Ig-bearing guinea-pig B lymphocytes - not only KLH-primed B cells but also unprimed B cells - are capable of presenting antigen to primed T cells in a major histocompatibility complex(MHC)-restricted fashion.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D000939:Epitopes; D005260:Female; D005802:Genes, MHC Class II; D006168:Guinea Pigs; D006433:Hemocyanins; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007106:Immune Sera; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; C029764:antimacrophage globulin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin