[Histologic characterization of hyaluronic acid in the cerebellum with hyaluronectin].
Two techniques of affino-immunofluorescence were described to localize hyaluronic acid (HA) on Rat cerebellum tissue sections. The first technique used the direct soluble hyaluronectin (HN)/anti-HN immune complex fixation to tissue-HA. In the second technique, HN fixation was followed by anti-HN antibody binding to HN. Both reactions were blocked by the addition of HA to HN/anti-HN complexes or to HN. The first direct technique is less time consuming and gives more clear-cut results than the second technique. These affino-immunological methods provide a better tool to localize HA in tissues than the classical stainings.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000032:analysis* / Q000378:metabolism; D051381:Rats
D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid
Caractérisation tissulaire de l'acide hyaluronique dans le cervelet par la hyaluronectine.