title: Human growth hormone radioiodination using different batches of 125I of various ages.
abstract: The reproducibility and the influence of the batch and decay level of Na125I on the radioiodination of human growth hormone (hGH) were examined by a polyacrylamide gel electrophoresis (PAGE) technique. The between-day coefficient of variation (CV) exhibited a value of 11.9% for labelling yields and 14.3% for antibody specific binding. The within-day variation for different shipments and isotope decay-levels was similar to that for the control experiment, carried out under the same conditions, using Na125I from a single lot. The results indicate that the decay-level and batch do not significantly influence either the yields or the immunological properties of the labelled product. The suitability of this PAGE technique as a control test for radioiodinated proteins is established by comparison with gel chromatography on Sephadex G-100.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D013006:Growth Hormone / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D046911:Macromolecular Substances; D011851:Radioactivity; D013997:Time Factors
chemical_list: D000939:Epitopes; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D013006:Growth Hormone