Comparative topo-optical investigation of cardiac glycoside localization.
The aldehyde-bisulfite-toluidine blue (ABT) reaction is a selective topo-optical test of vicinal-OH and amino-OH groups. The localization of cardiac glycoside was investigated morphologically. After digoxin the sarcolemma membranes, capillaries and sinus node showed strong basophilia and negative birefringence. The topo-optical reaction are useful for the histopathological examination. The new method gives the possibility to the digoxin intoxication with a high specificity.
D000818:Animals; D002301:Cardiac Glycosides / Q000032:analysis* / Q000506:poisoning; D004077:Digoxin / Q000032:analysis; D004285:Dogs; D005554:Forensic Medicine; D009206:Myocardium / Q000473:pathology*; D013194:Staining and Labeling
D002301:Cardiac Glycosides; D004077:Digoxin