Immunological identification of soluble carbonyl reductase with testosterone 17 beta-dehydrogenase (NADP+) in guinea pig liver and kidney.
Antiserum was produced against one of two carbonyl reductases purified from guinea pig liver cytosol to identify the enzymes as testosterone 17 beta-dehydrogenase isozymes. Immunoelectrophoresis and immunoprecipitation with the antiserum indicated that the two reductases had common antigenic sites. The antiserum inhibited most of both carbonyl reductase and testosterone 17 beta-dehydrogenase activities in the purified reductases and in cytosols of liver and kidney.
D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000429:Alcohol Oxidoreductases / Q000032:analysis* / Q000276:immunology; D000818:Animals; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006168:Guinea Pigs; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology
D000939:Epitopes; D015067:17-Hydroxysteroid Dehydrogenases; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+); C032690:3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+))