Action of murine gamma (immune)interferon on beta (fibroblast)-interferon resistant L 1210 and embryonal carcinoma cells.
Treatment of leukaemic mouse L 1210 S cells with beta or gamma interferon results in the development of antiviral and anticellular effects, both of which are also induced by interferon in L 1210 R cells selected for their resistance to gamma-interferon. Here we show that treatment of L 1210 S cells with beta or gamma interferon results in the induction of two double-stranded (ds) RNA dependent enzymes which have been described previously in beta and gamma-interferon treated mouse L-929 cells: pppA (2'p5'A)n synthetase and protein kinase(s). Treatment of L 1210 R cells with beta-interferon does not result in the induction of any detectable enhanced pppA (2'p5'A)n synthetase and protein kinase activities whereas gamma-interferon is capable of inducing both of these enzymes. These results indicate that although beta and gamma-interferon induce similar intracellular events, they may have different mechanism of interaction with their target cells. In addition, here we show that similar to the action of beta-interferon, treatment of mouse embryonal carcinoma cells with gamma-interferon results in the induction of pppA (2'p5'A)n synthetase in the absence of any detectable protein kinase activity or any apparent development of antiviral and anti-cellular effects. The significance of this in relation to the mechanism of action of interferon is discussed.
D015088:2',5'-Oligoadenylate Synthetase; D000818:Animals; D002478:Cells, Cultured; D004790:Enzyme Induction; D007372:Interferons / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000201:enzymology; D008081:Liposomes / Q000494:pharmacology; D051379:Mice; D009713:Nucleotidyltransferases / Q000096:biosynthesis*; D010766:Phosphorylation; D011494:Protein Kinases / Q000096:biosynthesis* / Q000378:metabolism; D013724:Teratoma / Q000201:enzymology; D014779:Virus Replication
D008081:Liposomes; D007372:Interferons; D011494:Protein Kinases; D009713:Nucleotidyltransferases; D015088:2',5'-Oligoadenylate Synthetase