[Immunological properties of an artificial antigen possessing the specificity of Salmonella O-factor 3].
For the first time the method of obtaining synthetic protein-free antigen with the specificity of Salmonella O-factor by the radical copolymerization of the synthetic trisaccharide 3-0 [4-0-(beta-D-mannopyranosyl)-alpha-L-rhamnopyranosyl]-beta-allyl-D- galactopyranoside with acrylamide was developed. The synthetic antigen thus obtained possessed the narrow specificity of serogroup E Salmonella O-factor 3. The serological activity of the antigen, studied in the precipitation and passive hemagglutination tests, was considerably higher than that of lipopolysaccharides isolated from S. anatum and S. newington. This synthetic antigen proved to be nontoxic and possessed immunogenic properties, inducing the formation of antibodies to Salmonella O-factor 3 in immunized animals.
D000178:Acrylamides / Q000138:chemical synthesis / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D007114:Immunization; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D019081:O Antigens; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012995:Solubility; D014312:Trisaccharides / Q000138:chemical synthesis / Q000276:immunology
D000178:Acrylamides; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D019081:O Antigens; D014312:Trisaccharides
Immunologicheskie svoĭstva iskusstvennogo antigena, obladaiushchego spetsifichnost'iu O-faktora 3 sal'monell.