The envelope proteins of bovine leukemia virus: purification and sequence analysis.
Two proteins, termed gp60 and p30, have been purified to homogeneity from bovine leukemia virus (BLV) using controlled pore glass and reverse-phase liquid chromatography (RPLC). gp60 was shown to be a glycoprotein by identification of glucosamine on the amino acid analyzer. Antiserum prepared to gp60 recognized in addition to gp60 a 52,000-Da polypeptide in some virus preparations, but did not cross-react with p30. The amino and carboxyl termini of gp60 were found to be tryptophan and arginine, respectively, and a 38-residue amino-terminal sequence of gp60 (NH2TrpArgXSerLeuSerLeuGlyAsnGlnGlnTrpMetThrAlaTyrAsnGlnGluAlaLys PheSerIleSerIleAspGlnIleLeuGluAlaHisAsnGlnSerProPhe-) was obtained. A 12-residue amino-terminal sequence for p30 (NH2SerProValAlaAlaLeuThrLeuGlySerAlaLeu) was also obtained. The p30 sequence showed substantial homology to the transmembrane proteins of both types B and C retroviruses and also to a deduced sequence of the 3' region of the env gene of human T-cell leukemia virus. From these results and from elution behavior of these proteins on RPLC, it was concluded that gp60 and p30 are the BLV env gene-encoded surface glycoprotein and transmembrane protein, respectively.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002685:Chiroptera; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification; D007106:Immune Sera; D007668:Kidney; D001909:Leukemia Virus, Bovine / Q000032:analysis*; D008168:Lung; D012190:Retroviridae / Q000032:analysis*; D012756:Sheep; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000302:isolation & purification*
D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D014759:Viral Envelope Proteins