title: Factors influencing human leukocyte adherence in vitro.
abstract: Studies were performed on factors influencing leucocyte adherence in vitro. Blood condensation was found to increase leukocyte adherence. Addition of heparin, dextran or ethanol caused a significant reduction of white blood cell count in blood samples in comparison with blood mixed with sodium EDTA or ACD solution. This suggests the existence of two granulocyte subpopulations; viz, rapidly adhering and slowly adhering. Heparin enhanced granulocyte adherence, while dextran and ethanol decreased it. Five-day storage of ACD blood led to a decrease in granulocyte adherence, while addition of heparin or histamine to ACD blood prevented this change to occur. The glucose concentration of 1,000 mg/dl augmented granulocyte adherence, while higher glucose concentrations induced its progressive fall below the control values. There was no significant change of lymphocyte adherence during the experiments.
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D001793:Blood Preservation; D002448:Cell Adhesion; D003911:Dextrans / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005898:Glass; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology*; D009757:Nylons
chemical_list: D000925:Anticoagulants; D003911:Dextrans; D009757:Nylons; D000431:Ethanol