Effects of calmodulin inhibitor on histamine release from rat peritoneal mast cells induced by concanavalin A and ionophore A23187.
The involvement of calmodulin in stimulus-secretion coupling of histamine release from rat mast cells was examined using a specific calmodulin inhibitor, W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide]. W-7 at low concentrations inhibited histamine release induced by either concanavalin A or ionophore A23187 and at high concentrations enhanced the release. But its congener, W-5 [N-(6-aminohexyl)-1-naphthalenesulfonamide], which has virtually no calmodulin-inhibiting activity, did not affect the release. It is concluded that W-7 modulates histamine release by inhibiting some functions of calmodulin in the cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000166:cytology; D000001:Calcimycin / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D003208:Concanavalin A / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D013449:Sulfonamides; D003208:Concanavalin A; D000001:Calcimycin; C017967:W 7