Changes in substance P concentrations after protein synthesis inhibition provide an index of substance P utilization.
In all but one brain region sampled, substance P-like immunoreactivity (SPLI) was unchanged 4 h after a dose of cycloheximide that produced a near-total inhibition of rat brain protein synthesis. This suggests that brain substance P (SP) utilization is slow under basal conditions. The administration of the dopamine antagonist haloperidol to cycloheximide-pretreated rats apparently accelerated striatonigral SP utilization, as evidenced by large depletions in SPLI in the substantia nigra and striatum. Measuring the decline of SPLI a short time after protein synthesis inhibition may provide an index of brain SP utilization.
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism*; D013378:Substantia Nigra / Q000187:drug effects* / Q000378:metabolism
D009419:Nerve Tissue Proteins; D013373:Substance P; D003513:Cycloheximide; D006220:Haloperidol; D004298:Dopamine