The functions of endogenous C1q, a subcomponent of the first component of complement, as a receptor on the membrane of macrophages.
C1q, the Fc-recognizing subcomponent of the first component of complement is synthesized by peritoneal macrophages. During the secretion phase C1q serves as an Fc-binding protein in the membrane of macrophages. The Fc-mediated rosette formation was inhibited in a dose-dependent manner when macrophages were pretreated with anti-C1q F(ab')2, whereas C3b rosette formation was not affected. Furthermore, preincubation of peritoneal macrophages with anti-C1q F(ab')2 abolished, dose- and time-dependently, the polyanion-mediated stimulation of secretion of lysosomal enzymes. Polyanion-induced enzyme release was prevented after incubation of polyanions with highly purified C1q. The inhibition of Fc receptor activity by polyanions (i.e. dextran sulfate, liquoid, polyvinyl sulfate) is completely reversed upon treatment of these macrophages with protamine. These findings are compatible with the hypothesis that C1q produced by macrophages serves in the macrophage membrane as an endogenous receptor for Fc and polyanionic molecules. Thus, C1q mediates cell-bound biological receptor functions before it is released from these cells and is incorporated into the macromolecular C1 complex.
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D003166:Complement Activating Enzymes / Q000096:biosynthesis / Q000276:immunology*; D015922:Complement C1q; D003911:Dextrans / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology*; D011487:Protein Conformation; D011951:Receptors, Complement / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology; D012397:Rosette Formation
D003911:Dextrans; D011951:Receptors, Complement; D011961:Receptors, Fc; D015922:Complement C1q; D002351:Carrageenan; D003166:Complement Activating Enzymes