[Effect of the antitumor antibiotic bleomycin on DNA polymerase activity].
Treatment with bleomycin activates considerably a repair synthesis of DNA in rat liver chromatin in vitro and can cause loosening of the nucleoprotein complex, which facilitates the accessibility or repair enzymes for lesions in chromatin DNA. The bleomycin action on DNA-template increases severalfold the rate of synthesis catalyzed by DNA polymerase beta inhibits the activity of DNA polymerase I from Escherichia coli and suppresses severalfold the activity of DNA polymerase alpha and DNA polymerase of bacteriophage T4. The effect of bleomycin consists in a prevailing increase of nicks and minimal gaps in DNA as compared to the rise of moderate gaps, thus suggesting that bleomycin is a gamma-mimetic.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002843:Chromatin / Q000187:drug effects / Q000201:enzymology*; D003090:Coliphages / Q000201:enzymology; D004256:DNA Polymerase I / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051381:Rats; D013698:Templates, Genetic
D002843:Chromatin; D001761:Bleomycin; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase
Deĭstvie protivoopukholevogo antibiotika bleomitsina na DNK-polimeraznuiu aktivnost'.