[Selective alkylation of G24 residue in tRNAPhe].
Alkylation of E. coli tRNAPhe with 4-(N-2-chloroethyl-N-methylamino) benzyl-5'-phosphamide of oligonucleotide d(pAACCA) was studied. G24 residue located near the sequence C17GGDA21 partially complementary to the oligonucleotide moiety of the reagent was shown to be alkylated. Oligonucleotide d(pAACCA) inhibited the alkylation. Association constant of oligonucleotide derivative with tRNAPhe (10(3) M-1) was evaluated from the dependence of the extent of tRNA modification on the concentration of the reagent. The reported method for selective alkylation of tRNA may be used for preparing photoaffinity derivatives of tRNA bearing an arylazidogroups in desired position.
D000478:Alkylation; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D004926:Escherichia coli / Q000032:analysis*; D019791:Guanidine; D006146:Guanidines; D006868:Hydrolysis; D012329:RNA, Bacterial / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*
D006146:Guanidines; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D019791:Guanidine
Selektivnoe alkilirovanie tRNAPhe po ostatku G24.