[Correlation between concentration of cytosol estradiol receptors and activities of RNA and protein synthesis in uterine myoma].
In an attempt to clarify the relationship between estradiol and uterine myoma, the concentration of the cytosol estradiol receptors (E2R) and the activities of RNA and protein syntheses (RS and PS) in uterine myoma and corresponding myometrium were measured. A 105,000 X g supernatant of tissue homogenate from which endogenous unbound steroid was removed by dextran-coated charcoal was used for the cytosol. Binding was measured by using the charcoal adsorption technique. RS and PS had been determined by the incorporation of 3H-Uridine and 14C-Leucine to the fresh tissue at 37 degrees C for 3 hours. The differences between paired samples of myoma and myometrium from the same uterus were statistically analyzed and the following results were obtained: The concentration of E2R in myoma was significantly higher than that in myometrium. But there was no significant difference in RS and PS between myoma and myometrium. There was a close correlation of the concentration of E2R, RS and PS between myoma and myometrium from the same uterus. The concentration of E2R did not correlate to RS and PS in myoma, whereas RS correlated with PS in myoma.
D000328:Adult; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism*; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis*; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000032:analysis*; D012588:Scintillation Counting; D014594:Uterine Neoplasms / Q000378:metabolism*
D009363:Neoplasm Proteins; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D012313:RNA