IR gene regulation of the response to trinitrophenyl-polysaccharides. Two independent genes are required for antibody production.
The primary in vitro antibody response to TNP-Ficoll was found to be under H-2-restricted Ir gene control. Strains B10(H-2b), B10.A(H-2a), and B10.S(9R) (H-2t4) were consistently low responders while strains D2.GD(H-2g2), B10.GD(H-2g2), and B10.S(H-2s) were high responders. The in vitro TNP-Ficoll response in congenic recombinant and F1 hybrid mice demonstrated the requirement for complementation of two independent Ir genes. One Ir gene mapped in or to the left of the I-A subregion with high responder alleles being s or d. The second Ir gene mapped to the right of the I-E subregion and required b or s alleles for complementation. These results were further supported by the ability to block the TNP-Ficoll response by appropriate anti-Ia serum pretreatment of the antigen-presenting macrophages. When a structurally different polysaccharide antigen TNP-dextran was used, an identical pattern of restriction was observed.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002478:Cells, Cultured; D003911:Dextrans / Q000276:immunology; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006824:Hybridization, Genetic; D007106:Immune Sera / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology; D008351:Mannans / Q000276:immunology; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D011995:Recombination, Genetic; D014301:Trinitrobenzenes / Q000276:immunology*
D003911:Dextrans; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D008351:Mannans; D009578:Nitrobenzenes; D011134:Polysaccharides; C026595:TNP-AECM-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll