Specific activity of carp antisera against beta 2-microglobulin (beta 2m) and evidence for a beta 2m homologue in carp (Cyprinus carpio).
Antisera against rabbit and human beta 2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for beta 2m, and detected beta 2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit beta 2m to distinguish between mouse and rat beta 2m's suggested that the carp itself carries beta 2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-beta 2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog beta 2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian beta 2m.
D000818:Animals; D000918:Antibody Specificity; D002347:Carps / Q000097:blood / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D013045:Species Specificity; D014714:Vertebrates / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology* / Q000302:isolation & purification
D000939:Epitopes; D001613:beta 2-Microglobulin