title: Comparison of antigenicity of toxins produced by Clostridium botulinum type C and D strains.
abstract: C1 neurotoxin of Clostridium botulinum strains C-Stockholm (C-ST), C beta-Yoichi, C-468, CD6F, and C-CB19 and type D toxin of strains D-1873 and D-CB16 were purified by gel filtration, ion exchange, and affinity chromatographies. The purified toxins had di-chain structure made of heavy and light chains. The toxins of C beta-Yoichi, C-468, CD6F, and C-CB19 reacted with anti-C-ST heavy chain and anti-C-ST light chain in immunodiffusion tests and enzyme-linked immunosorbent assay, whereas D-CB16 toxin reacted with anti-D-1873 heavy chain and anti-D-1873 light chain. However, C-6813 toxin reacted with anti-D-1873 heavy chain and anti-C-ST light chain but not with anti-C-ST heavy chain or anti-D-1873 light chain immunoglobulin G. These results indicate common antigens in the heavy chains of C-6813 and D-1873 toxins and in the light chains of C-6813 and C-ST toxins. Further, they provide evidence for heterogeneity within type C1 toxin subunits.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001905:Botulinum Toxins / Q000276:immunology* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003014:Clostridium botulinum / Q000145:classification / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005779:Immunodiffusion; D007142:Immunoglobulin gamma-Chains / Q000032:analysis; D051379:Mice; D009498:Neurotoxins / Q000276:immunology / Q000302:isolation & purification; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D007142:Immunoglobulin gamma-Chains; D009498:Neurotoxins; D001905:Botulinum Toxins