Binding of a rhodamine-labeled lipopolysaccharide to lipopolysaccharide-responder and nonresponder lymphocytes.
The binding levels of a highly soluble rhodamine-labeled lipopolysaccharide (LPS-Rh) on different LPS-responsive and nonresponsive murine spleen cells were measured with a cytofluorometer (CFM). In all the experiments, a preferential binding of LPS-Rh to LPS-responsive cells was observed. Furthermore, only a proportion of B cells from the responder mouse strain was selectively stained after addition of LPS-Rh. These findings suggested that LPS-Rh binds to specific components of the cell membrane, involved in the triggering of B-cell mitogenicity.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D005434:Flow Cytometry; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000502:physiology; D012235:Rhodamines / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000378:metabolism
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008934:Mitogens; D011971:Receptors, Immunologic; D012235:Rhodamines