Photolabelling of dopamine-beta-hydroxylase by bleomycin.
Irradiation of a mixture of dopamine-beta-hydroxylase and bleomycin with light of wavelengths greater than 300 nm. leads to irreversible inhibition which is not prevented in the presence of tyramine. Prephotolysis of bleomycin followed by incubation (in the dark) with dopamine-beta-hydroxylase did not lead to irreversible inhibition. Thus bleomycin is a photolabel of this enzyme.
D001761:Bleomycin / Q000494:pharmacology* / Q000528:radiation effects; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors* / Q000528:radiation effects; D008027:Light; D010777:Photochemistry; D010782:Photolysis
D001761:Bleomycin; D004299:Dopamine beta-Hydroxylase