Lucifer yellow, neuronal marking and paraffin sectioning.
An unusual procedure with the dye lucifer yellow has provided stable neuronal marking that survives paraffin embedding and sectioning. Lucifer yellow CH was dissolved in an electrolyte containing formaldehyde and injected into the large interneurons of a cricket. Intense fluorescence in the axoplasm was retained even after conventional histological procedures.
D000818:Animals; D005404:Fixatives; D005453:Fluorescence; D005557:Formaldehyde; D007395:Interneurons / Q000033:anatomy & histology; D007546:Isoquinolines; D008867:Microtomy; D009474:Neurons / Q000033:anatomy & histology*; D009987:Orthoptera / Q000033:anatomy & histology*; D010232:Paraffin; D013194:Staining and Labeling
D005404:Fixatives; D007546:Isoquinolines; D005557:Formaldehyde; D010232:Paraffin; C017475:lucifer yellow