A method to detect 2-keto-3-deoxyoctanat and related compounds on pherograms and chromatograms.
A variation of the thiobarbituric acid spray reagent to detect 2-keto-3-deoxyoctanat (KDO), KDO derivatives, 2-deoxysugars, and N-acetylneuraminic acid on pherograms and chromatograms is described. Using all reagents in organic solvents and developing the color at room temperature, accurate locations of the spots without spreading and a low background stain are achieved.
D002855:Chromatography, Thin Layer; D004590:Electrophoresis, Paper; D013194:Staining and Labeling; D013400:Sugar Acids / Q000032:analysis*; D013858:Thiobarbiturates
D013400:Sugar Acids; D013858:Thiobarbiturates; C002532:2-keto-3-deoxyoctonate; C029684:thiobarbituric acid