[Studies on determination of alpha-amylase with p-nitrophenyl-alpha-D-maltotetraoside].
Nitrophenylmaltodextrins are alpha-amylase substrates which allow a continuous determination with a zero order kinetics over a period of at least 10 min, without deviations from linearity. Only one auxiliary enzyme is necessary. Practicability and clinical evidence of alpha-amylase determinations by means of p-nitrophenyl-alpha-D-maltotetraoside are demonstrated. The interserial precision of 0.84% cannot conceal an only moderate correlation with previous methods. This fact, however, does not negate the advantages.
D000681:Amylases / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D009844:Oligosaccharides; D000516:alpha-Amylases / Q000032:analysis* / Q000097:blood / Q000652:urine
D009844:Oligosaccharides; C037063:4-nitrophenyl-alpha-maltotetraose; D000681:Amylases; D000516:alpha-Amylases
Untersuchungen zur Bestimmung der alpha-Amylase mit p-Nitrophenyl-alpha-D-maltotetrosid.