Identification of the microtubule-associated protein map2 and its binding sites on metaphase chromosomes from cultured cells.
The microtubule-associated protein MAP2, which binds preferentially to AT sequences of DNA, can bind to metaphase chromosomes. The binding pattern of MAP2 to chromosomes is similar to that found for the binding of the bisbenzimidazole derivative 33258 Hoechst, which also binds preferentially to AT-rich regions.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D006690:Bisbenzimidazole / Q000378:metabolism; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism*; D007668:Kidney / Q000166:cytology; D008677:Metaphase; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013552:Swine; D014404:Tubulin / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D014404:Tubulin; D006690:Bisbenzimidazole