In vivo 19F-NMR of 5-fluorouracil incorporation into RNA and metabolites in Escherichia coli cells.
19F resonances from RNA with 5-fluorouracil incorporated could be observed in intact Escherichia coli cells, as well as in tRNA isolated from the cells. 19F-NMR signals from the metabolic breakdown products of the fluorinated RNA were also detected in vivo. By observing the 19F-NMR spectrum, variations in the metabolic disposition of administered 5-fluorouracil could be monitored as a function of time and be compared when the cells were deprived of oxygen and other nutrients, subjected to ethidium bromide treatment, or grown in the presence of mitomycin C.
D004926:Escherichia coli / Q000378:metabolism*; D004996:Ethidium / Q000494:pharmacology; D005472:Fluorouracil / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008937:Mitomycins / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism*
D008937:Mitomycins; D012329:RNA, Bacterial; D004996:Ethidium; D005472:Fluorouracil