Affinity chromatography used in distinguishing alpha-fetoprotein in serum from patients with tumors of hepatic parenchyma and of germ cells.
We used affinity chromatography on concanavalin A Sepharose to study the serum alpha-fetoprotein of 10 patients with histologically proven germ-cell tumors and 12 patients with primary liver cancer. Less than 50% of the fetoprotein from germ-cell tumors bound to concanavalin A, as compared with more than 80% of the alpha-fetoprotein from primary liver cancers.
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood*; D008297:Male; D008649:Mesonephroma / Q000097:blood*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood*; D012685:Sepharose; D013736:Testicular Neoplasms / Q000097:blood*; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D003208:Concanavalin A; D012685:Sepharose