Determination of immunoglobulin class and subclass of monoclonal antibodies to human alpha-fetoprotein by solid phase radioimmunoassay.
A solid phase radioimmunoassay using the incubation sequence: mouse immunoglobulin (Ig) rabbit anti-mouse Ig, monoclonal antibody and 125I-labeled antigen was performed to determine the class and subclass of seven murine monoclonal antibodies against human alpha-fetoprotein. All antibodies belonged to the IgG class. The subclass type was IgG 2a for four antibodies, IgG 1 for two antibodies and IgG 2b for one antibody.
D000911:Antibodies, Monoclonal / Q000145:classification*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000145:classification; D007136:Immunoglobulins / Q000145:classification*; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007136:Immunoglobulins; D000509:alpha-Fetoproteins