Characteristics of basophil histamine release by neuromuscular blocking drugs in patients with anaphylactoid reactions.
Histamine release from the basophil leucocytes of patients with apparently non-immune, anaphylactiod reactions to neuromuscular blockers ( NMB ) has been studied in vitro. Substantial release was obtained in the majority of patients with clinical reactions, with little or no release in normal subjects. The chemical specificity and selectivity varied in individual patients: each individual reacting to one agent or a particular combination of NMB drugs. The test was of considerable diagnostic and predictive value. We studied some of the characteristics of the histamine release process and the effect of certain modulators. The findings strongly suggest a non-cytotoxic secretory process requiring the presence of two quaternary ammonium groups as suggested by the rarity of release with tubocurarine; inhibition of succinyldicholine (suxamethonium)-induced histamine release by serum cholinesterase treatment, acetylcholine and tubocurarine; and the bell-shaped dose-response curve, particularly with suxamethonium. Histamine release by all NMB was completely inhibited in calcium-free medium, and markedly potentiated by deuterium oxide, a microtubule stabilizer. Both tachyphylaxis (by prior exposure to the specific agent in absence of calcium), and cross-tachyphylaxis with anti-IgE were elicited.
D000109:Acetylcholine / Q000494:pharmacology; D000707:Anaphylaxis / Q000378:metabolism*; D001491:Basophils / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002802:Cholinesterases / Q000097:blood; D003903:Deuterium / Q000494:pharmacology; D017666:Deuterium Oxide; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D009466:Neuromuscular Blocking Agents / Q000494:pharmacology*; D014403:Tubocurarine / Q000494:pharmacology; D014867:Water / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D009466:Neuromuscular Blocking Agents; D014867:Water; D003903:Deuterium; D002802:Cholinesterases; D017666:Deuterium Oxide; D000109:Acetylcholine; D014403:Tubocurarine