Granulocyte specificities detected by cytotoxicity and agglutination.
In a sample of 100 individuals from the Prague population antigenic specificities of granulocytes were determined on the basis of agglutination activity by means of standard typing sera (Bethesda, MD). The specificities NA1, NA2, NB1 were detected in 59, 87, and 90 percent with gene frequency of 0.360, 0.640, and 0.684, respectively. One serum from a pregnant woman reacted with 76 percent of persons up to the titer 8 or 16. When correlating this serum with N typing sera, the best match was found for anti-NA2 serum (p less than or equal to 0.01). On the basis of cytotoxic activity, using specific granulocytotoxic sera obtained by screening sera from pregnant women and multitransfused patients, two antigenic specificities were detected. The incidence of G1 and G2 was 55 and 67 percent and the gene frequency 0.555 and 0.455, respectively. The comparison of specificities based both on agglutination and cytotoxic activity showed a significant association of G1 with NA2 and G2 with NA1 (p less than or equal to 0.001).
D000372:Agglutination Tests; D000373:Agglutinins / Q000032:analysis; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins / Q000032:analysis; D000939:Epitopes / Q000032:analysis* / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D008297:Male; D010375:Pedigree; D010641:Phenotype; D011247:Pregnancy
D000373:Agglutinins; D003603:Cytotoxins; D000939:Epitopes