title: Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat).
abstract: A family of tandemly repeated sequences, having a basic repeating unit of 741 base-pairs, has been identified in Xenopus laevis DNA and designated satellite 1. Apart from its rather long repeat unit, the characteristics of this DNA appear to be quite similar to those of complex satellite DNAs from other organisms. The nucleotide sequence of a cloned repeat unit shows no evidence of simpler internal repeats, and there is no obvious suggestion of reasonable RNA- or protein-coding regions. Transcripts homologous to this DNA could not be demonstrated in liver, embryo or oocyte RNAs. By blot-hybridization, satellite 1 has been shown to exist in the genome chiefly as tandem repeats of the 741 base-pair sequence. However, there are a number of repeats that differ from the normal sequence (as judged by loss or gain of restriction sites) and some that differ in length. Similar variants are often, but not always, clustered. Characterization of genomic clones of this satellite has confirmed the tandem organization and clustering of variants. The nature of some variants has been elucidated in more detail. Some regions of the basic repeat seem to be more prone to variation than are others.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005260:Female; D008297:Male; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012313:RNA; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D004276:DNA, Satellite; D009692:Nucleic Acid Heteroduplexes; D012313:RNA