Calcium in myocardial tissue and isolated mitochondria from a teleost.
A method is presented for isolating cardiac mitochondria from bony-fish. Calcium levels in ventricular whole tissue and isolated mitochondria of Gadus virens L. are determined by atomic absorption flame spectroscopy, and were found to be about 8 and 16 nmolCa/mg prot., respectively. In conclusion, the calcium concentration within the myocardial mitochondria in this species may be nearly three times higher than at the outside, and probably these structures serve as a "calcium sink". The results are compared with those previously reported for mammals.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012430:Ruthenium Red; D013194:Staining and Labeling
D012430:Ruthenium Red; D002118:Calcium