In vitro release of 5-[3H]hydroxytryptamine from rat spinal trigeminal nucleus.
A potassium-evoked release of 5-[3H]hydroxytryptamine (5-HT), newly synthesized from [3H]tryptophan, was obtained from slices of the subnucleus caudalis of rat spinal trigeminal nucleus by means of an in vitro perfusion system. This release was dose-dependent and calcium-sensitive and quantitatively comparable to the 5-HT release in substantia nigra. The putative transmitter of the primary sensory afferents, substance P, stimulates the spontaneous 5-HT release, whereas another peptide, neurotensin, has no effect.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D009496:Neurotensin / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology; D014279:Trigeminal Nucleus, Spinal / Q000378:metabolism*
D012701:Serotonin; D013373:Substance P; D009496:Neurotensin; D011189:Potassium Chloride; D002118:Calcium