Inhibitors of DNA synthesis (aphidicolin and araC/HU) prevent the recovery of RNA synthesis after UV-irradiation.
UV-irradiation causes an immediate depression in the rate of RNA synthesis in human skin fibroblasts. RNA synthesis rates recover to greater than or equal to 90% of unirradiated levels within 90 min in normal cells. This recovery can be prevented by incubating the cells after irradiation with araC/HU or aphidicolin, potent inhibitors of DNA replication and excision repair. The effect of these inhibitors on the recovery of RNA synthesis can also be observed in non-dividing cells; it is thus independent of their effects on DNA replication.
D016590:Aphidicolin; D002453:Cell Cycle; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*; D014466:Ultraviolet Rays
D004224:Diterpenes; D003561:Cytarabine; D016590:Aphidicolin; D012313:RNA; D006918:Hydroxyurea