[3H]substance P binding in the intermediolateral cell column and striatum of the rat.
[3H]substance P binding was studied in the intermediolateral cell column and striatum in the rat using slide-mounted sections. The intermediolateral cell column had a single high affinity binding component with a dissociation constant, Kd = 1.45 nM and the number of sites, Bmax = 18.1 fmol per mg protein. The striatum had a Kd = 0.77 nM and a Bmax = 23.5 fmol per mg protein. The relative potency of various substance P-like tachykinins in displacing [3H]substance P suggested that both these areas may contain a substance P-P (for physalaemin) receptor subtype. (D-Pro4, D-Trp7,9)substance P(4-11), a substance P antagonist, has a relatively low affinity (micromolar range) in both these areas.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013373:Substance P / Q000378:metabolism*
D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P