In-vivo interaction of some organophosphorus insecticides with different biochemical targets in white rats.
A comparative study between five organophosphorus insecticides: Leptophos, EPN, Cyanofenphos, Chlorpyrifos and Diazinon, was carried out for acute oral toxicity to white rats and for their in vivo interaction at 1/10th of LD50 doses with the activity of six serum enzymes after 4 wks from oral administration. Leptophos, Chlorpyrifos and diazinon exerted significant inhibition particularly to glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), glutamyltransferase (GT) and lactate dehydrogenase (LDH). Adding ascorbic acid in the diet at 0.5% enhanced the acute oral toxicity of leptophos, chlorpyrifos and diazinon. For all the compounds, presence of ascorbic acid protected a number of the monitored serum enzymes from being inhibited except for leptophos. Ascorbic acid caused hypoglycemia with sublethal doses of leptophos, chlorpyrifos, and diazinon. The synergist piperonyl butoxide alone at 750 mg/kg dose inhibited the activity of the six serum enzymes. Presence of ascorbic acid in the diet intensified the inhibitory effect of piperonyl butoxide to all enzymes except for GOT.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001205:Ascorbic Acid / Q000009:adverse effects; D001786:Blood Glucose / Q000378:metabolism; D004390:Chlorpyrifos / Q000009:adverse effects; D002802:Cholinesterases / Q000032:analysis; D003976:Diazinon / Q000009:adverse effects; D006003:Glycogen / Q000378:metabolism; D007306:Insecticides / Q000009:adverse effects*; D010773:Leptophos / Q000009:adverse effects; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009946:Organothiophosphorus Compounds / Q000009:adverse effects; D004849:Phenylphosphonothioic Acid, 2-Ethyl 2-(4-Nitrophenyl) Ester / Q000009:adverse effects; D011506:Proteins / Q000378:metabolism; D051381:Rats; D000637:Transaminases / Q000032:analysis
D001786:Blood Glucose; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D011506:Proteins; C002488:cyanophenphos; D004849:Phenylphosphonothioic Acid, 2-Ethyl 2-(4-Nitrophenyl) Ester; D006003:Glycogen; D010773:Leptophos; D000637:Transaminases; D002802:Cholinesterases; D000469:Alkaline Phosphatase; D004390:Chlorpyrifos; D001205:Ascorbic Acid; D003976:Diazinon