title: New rapid technique for counting microorganisms directly on membrane filters.
abstract: The proposed technique is a modification of classical procedures for counting micoorganisms directly on membrane filters. The technique consists of clearing the filter with immersion oil, paraffin oil or cedar oil prior to staining with crystal violet, carbol fuchsin or malachite green. Millipore filters (0.1 micron pore size, VC type) were found to be superior to other filters with regard to the contrast between microorganisms and filter surface.
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000295:instrumentation; D005840:Gentian Violet; D006088:Gram-Negative Aerobic Bacteria / Q000302:isolation & purification; D008851:Micropore Filters; D009821:Oils; D012394:Rosaniline Dyes; D012722:Sewage / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*; D014871:Water Microbiology
chemical_list: D009821:Oils; D012394:Rosaniline Dyes; D012722:Sewage; C005095:malachite green; C006898:carbol-fuchsin solution; D005840:Gentian Violet