title: Proteolytic activity and immunoreactivity of chemically modified papain.
abstract: The effect of different chemical modifications of papain on the proteolytic activity and immunoreactivity has been studied. Modification with Dextran T 2000 caused increasing decline in proteolytic activity both with increasing degree of dextran oxidation and amount of bound dextran, whilst the immunoreactivity determined by nephelometry remained unchanged. Modification of papain with glutaraldehyde and formaldehyde causes rapid drops in both activities, even at very low concentrations of agents. Acetylation of papain showed expressive maxima of both proteolytic and immunochemical activities at the same degree of enzyme acetylation. Modification with diazobenzenesulfonic acid caused a high increase in immunoreactivity and a small increase in proteolytic activity.
mesh_terms: D000089:Acetic Anhydrides; D055598:Chemical Phenomena; D002621:Chemistry; D003911:Dextrans; D005557:Formaldehyde; D005976:Glutaral; D006454:Hemoglobins / Q000378:metabolism; D010206:Papain / Q000276:immunology / Q000378:metabolism*
chemical_list: D000089:Acetic Anhydrides; D003911:Dextrans; D006454:Hemoglobins; D005557:Formaldehyde; C031800:acetic anhydride; D010206:Papain; D005976:Glutaral