title: Location of DNA sequences complementary to small nuclear repeat RNA (fr 3-RNA) in relation to DNA sequences coding for albumin and alpha-fetoprotein in rat liver cells.
abstract: Rat liver nuclei contain a 29-nucleotides-long RNA (fr 3-RNA) which is transcribed from middle repetitive DNA sequences. By Southern analysis of restriction fragments of rat albumin and alpha-fetoprotein genomic clones, DNA sequences complementary to this RNA were detected on a 4.6 kbp Eco RI fragment located 600 bp downstream from the termination exon of the albumin gene and on a 2 kbp Eco RI-HindIII fragment located 10 kbp downstream from the restriction fragment containing the alpha-fetoprotein site. No sequence complementary to this RNA was found either in the introns of exons of both genes or in the regions extending 7 kbp upstream from the first albumin exon and 10 kbp upstream of the first alpha-fetoprotein exon. We concluded that sequences complementary to fr 3-RNA are present at the 3'-end flanking regions of the rat albumin and alpha-fetoprotein gene complexes.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005796:Genes; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D012342:RNA, Small Nuclear; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012709:Serum Albumin / Q000235:genetics*; D014158:Transcription, Genetic; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D012342:RNA, Small Nuclear; D012709:Serum Albumin; D000509:alpha-Fetoproteins; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes