Some rat sensory neurons in culture express characteristics of differentiated pain sensory cells.
Sensory neurons were dissociated from trigeminal ganglia or from dorsal root ganglia of rats, grown in culture, and examined for expression of properties of pain sensory cells. Many sensory neurons in culture are excited by low concentrations of capsaicin, reportedly a selective stimulus for pain sensory neurons. Many are excited by bradykinin, sensitized by prostaglandin E2, or specifically stained by an antiserum against substance P. These experiments provide a basis for the study of pain mechanisms in cell culture.
D000818:Animals; D000831:Animals, Newborn; D001920:Bradykinin / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D015232:Dinoprostone; D005727:Ganglia, Spinal / Q000503:physiopathology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010146:Pain / Q000503:physiopathology*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D013373:Substance P / Q000494:pharmacology; D014276:Trigeminal Nerve / Q000503:physiopathology*
D011458:Prostaglandins E; D013373:Substance P; D015232:Dinoprostone; D002211:Capsaicin; D001920:Bradykinin