[Correlation between the rate of protein and nucleic acid synthesis and the intensity of various glucose metabolic pathways in rat liver cells].
A sharp and strong suppression of protein synthesis by cycloheximide in liver cells of starving rats is paralleled with activation of RNA synthesis and glucose-6-phosphate dehydrogenase production. Subsequent reconstitution and stimulation of protein synthesis (6-12 hrs after cycloheximide injection) result in activation of hexokinase. Upon stimulation of DNA synthesis (48-60 hrs after cycloheximide injection) the activity of both enzymes is very low. Since glucose-6-phosphate dehydrogenase appears to be the limiting step of glucose decay via the pentose phosphate pathway, and hexokinase is the limiting step of glycolysis, it was assumed that RNA synthesis predominantly occurs via the pentose phosphate pathway, while that of proteins via glycolysis.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006593:Hexokinase / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism; D010428:Pentosephosphates / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013217:Starvation / Q000378:metabolism
D001786:Blood Glucose; D007328:Insulin; D008112:Liver Glycogen; D010428:Pentosephosphates; D012313:RNA; D004247:DNA; D003513:Cycloheximide; D006593:Hexokinase; D005947:Glucose
Sootnoshenie skorosti sinteza belkov i nukleinovykh kislot i intensivnosti razlichnykh puteĭ metabolizma gliukozy v kletkakh pecheni krys.