Demonstration of antigenic determinants specific for the split products of the third complement factor, C3.
Crossed anti-C3c immunoelectrophoresis of a plasma sample with in vivo complement activation revealed a pronounced 'spur' formation towards the cathodic region of immunochemical interaction between native C3 and C3c. In situ absorption of the antibody preparation against C determinants of the 3rd complement factor using fresh normal EDTA plasma as antigen demonstrated the presence of C3 split product specific determinants.
D000917:Antibody Formation; D011232:Chemical Precipitation; D003176:Complement C3 / Q000276:immunology; D015932:Complement C3c; D000939:Epitopes; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional
D003176:Complement C3; D000939:Epitopes; D015932:Complement C3c