Dog serum albumin as an allergen. IgE, IgG and lymphocyte responses in dog dander-sensitive asthmatic children.
To study the allergenicity of dog serum albumin (DSA) in dog-sensitive subjects, we investigated 203 asthmatic children by means of the skin prick test (SPT), the radioallergosorbent test (RAST) and the in vitro lymphocyte stimulation test. Significant SPT reactions to DSA were observed in only 9 out of 80 subjects with a significantly positive SPT reaction to dog dander and hair (DDH). Analogously, positive DSA RAST results were observed only in a minority of the cases with a positive DDH RAST. Significant SPT reactions to DSA were not observed in subjects with a negative clinical history of dog allergy, or in subjects with a negative reaction to DDH in the SPT or in the provocation test. DSA induced remarkable lymphocyte stimulation in only 1 subject, who also had a significant SPT reaction to DSA. DSA-specific serum IgG antibody titers measured by ELISA were not found to correlate with DSA SPT or DSA RAST.
D000293:Adolescent; D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D001249:Asthma / Q000175:diagnosis / Q000276:immunology; D002415:Cats; D002648:Child; D002675:Child, Preschool; D004285:Dogs; D000939:Epitopes; D005260:Female; D006197:Hair / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D011852:Radioallergosorbent Test; D012709:Serum Albumin / Q000032:analysis / Q000276:immunology*; D012882:Skin Tests
D000485:Allergens; D000939:Epitopes; D007074:Immunoglobulin G; D012709:Serum Albumin; D007073:Immunoglobulin E