title: Incident light microscopy of normal and transformed cultured fibroblasts stained with coomassie blue R250.
abstract: Incident light microscopy of fibroblasts stained with Coomassie blue R250 results in images of the cytoskeleton with a similar high contrast to those obtained by specific immunofluorescence microscopy. This technique is especially useful for the routine visualization of the cytoskeleton of transformed cells.
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts; D005354:Fibrosarcoma; D051379:Mice; D008853:Microscopy / Q000379:methods*; D012394:Rosaniline Dyes; D013194:Staining and Labeling
chemical_list: D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R