Induction of tolerance by haptenated liposomes carrying mouse erythrocyte membrane glycoprotein.
Membrane sialoglycoprotein (GP) of mouse erythrocytes were incorporated into artificial membranes of liposomes which also contained DNP-aminocaproylphosphatidylethanolamine hapten. The liposomes were tested for their potential to modulate the anti-hapten antibody response in mice as a plausible model of haptenated isologous erythrocytes which is known to be a potent tolerogen. Repeated intravenous inoculation with DNP-liposomes carrying mouse GP resulted in a significant suppression of splenic plaque-forming cells and serum antibody produced in the recipient mice against the subsequent immunization with DNP-KLH in saline or DNP-HGG in Freund's complete adjuvant, in a strictly hapten-specific fashion. The suppression was long-lasting, and the secondary response to DNP-KLH given over a month later was also affected. DNP-liposomes without GP, or those with rabbit GP, exhibited little or no suppressive effect. The observed capacity of mouse GP-bearing DNP-liposomes was reminiscent of that of haptenated mouse erythrocytes in that only the IgG response, but not the IgM response, was suppressed.
D000818:Animals; D000917:Antibody Formation; D000939:Epitopes / Q000032:analysis; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007108:Immune Tolerance; D008081:Liposomes / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010714:Phosphatidylethanolamines / Q000276:immunology; D012795:Sialoglycoproteins / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; C021015:2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine; D000939:Epitopes; D006241:Haptens; D008081:Liposomes; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D012795:Sialoglycoproteins; D014301:Trinitrobenzenes; C030730:dinitrophenyl gamma globulin; D005719:gamma-Globulins; D006433:Hemocyanins