Silver staining of cerebrospinal fluid IgG in isoelectric focusing gels.
A silver staining technique to be used on isoelectric focusing (IEF) gels for cerebrospinal fluid (CSF) oligoclonal IgG bands is described. The technique provides a sensitivity for detection of greater than 0.17 micrograms of IgG. The additional use of immunofixation with anti-human IgG antiserum rather than chemical fixation improves the sensitivity two-fold and permits identification of the oligoclonal bands as IgG. The technique enables one to focus small volumes of unconcentrated CSF (less than 40 microliters) and provides a powerful research tool.
D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007136:Immunoglobulins / Q000134:cerebrospinal fluid; D007525:Isoelectric Focusing / Q000379:methods; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D043025:Oligoclonal Bands; D012394:Rosaniline Dyes; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods
D007074:Immunoglobulin G; D007136:Immunoglobulins; D043025:Oligoclonal Bands; D012394:Rosaniline Dyes; D012835:Silver Nitrate; C004692:coomassie Brilliant Blue