Isolation and characterization of Streptomyces erythreus plasmids.
Streptomyces erythreus strains were found to carry several plasmids of molecular weights ranging from about 2 X 10(6) Mr to 40 X 10(6) Mr. Restriction enzyme maps for the streptomycete plasmids pPC7 and pPC8 were constructed for the enzymes Bg/II, EcoRI, XbaI, HindIII, BamHI and SalI. The smaller, pPC8, plasmid appears to be a naturally occurring deletion variant of pPC7. These plasmids belong to the group of conjugative streptomycete plasmids.
D003470:Culture Media; D046508:Culture Techniques; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004269:DNA, Bacterial / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000302:isolation & purification; D008970:Molecular Weight; D010641:Phenotype; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*
D003470:Culture Media; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes