2'-Phosphodiesterase activity in human cell lines treated or untreated with human interferon.
2'-Phosphodiesterase activity was investigated, by measuring either the disappearance of (2',5')oligo(adenylate) or the release of 5'AMP, in several human cell lines (RSa, IFr, HEC-1, WGAr and HeLa) possessing different sensitivities to interferon, and treated or untreated with human interferon. In various cell lines whose (2'-5')oligo(adenylate) synthetase was normally induced by interferon treatment, both kinetic studies and measurements at different enzyme concentrations indicated that 2'-phosphodiesterase activity remained unchanged after interferon treatment. This observation was confirmed over a broad range of substrate concentrations (1-25000 nM). The activity of 2'-phosphodiesterase was dependent on Mg(OAc)2. Our results indicate that in various human cell lines the modulation of (2'-5')oligo(adenylate) metabolism by interferon does not involve an increase of 2'-phosphodiesterase activity.
D000227:Adenine Nucleotides / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D000249:Adenosine Monophosphate; C023505:2',5'-oligoadenylate; D007372:Interferons; D010727:Phosphoric Diester Hydrolases