Translation of lymphocyte mRNA for mouse colony stimulating factor.
Poly A+ RNA has been purified from phorbol myristate acetate stimulated mouse EL4 cells. Translation, by microinjection into frog oocytes, gave biologically active interleukin 2 (IL2) and colony stimulating factor for granulocytes and macrophages (GM-CSF). These two mRNAs were separated by centrifugation through linear sucrose gradients. The sedimentation coefficients for IL2 and GM-CSF mRNAs were found to be 11.5S and 8S respectively. The clonal and cellular morphologies induced by the oocyte material corresponded to low concentrations of authentic GM-CSF.
D000818:Animals; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000235:genetics*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003115:Colony-Stimulating Factors; D010704:Phorbols; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D013755:Tetradecanoylphorbol Acetate