Perspectives for achieving improved information by the observation of thin sections in the electron microscope.
Obtaining biologically significant fine detailed information from sections is limited firstly by the unknown distribution of heavy metal stain and secondly by conformational changes induced by dehydration. Only afterwards we have to be concerned with beam induced alterations. By Z-imaging in a STEM, completely unstained material can now become imaged sharply with high contrast. By this the first limitation is eliminated and the second can become explored. For this purpose new resins designed for low temperature embedding might become important. First biological results are presented which illustrate the potential of these techniques: The transmembrane protein of the separate junction has been revealed as such and shown that only the hydrophilic part of the protein is stained by uranyl acetate. This type of staining therefore does not allow the detection of any transmembrane proteins in sections.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D004331:Drosophila melanogaster / Q000648:ultrastructure; D004926:Escherichia coli / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron / Q000379:methods; D013194:Staining and Labeling
D008565:Membrane Proteins