Cell-surface antigenic modifications with trinitrophenyl sulfonate versus trifluoromethyl-dinitrophenyl sulfonate.
The aim of the work was to justify the use of trifluoromethyl-dinitrobenzene sulfonate (CF3-DNBS) modification rather than trinitrobenzene-sulfonate (TNBS) modification, so as to be able to take advantage of the presence of fluorine atoms in the analogue, which allow the analysis of the hapten-carrier bonds by 19F-NMR nuclear magnetic resonance. Cell-surface antigenic modifications brought about by exposure to TNBS or CF3-DNBS were found to be immunologically cross-reactive, both in cell-mediated lymphocytotoxicity and in indirect immunofluorescence. The extent of haptenic derivatizations was found to be of the same order of magnitude, as appraised both by quantitative-absorption studies or by using radioactive hapten, provided that the less chemically reactive CF3-DNBS was used at the concentration of 10 mM and TNBS at the concentration of 1 mM. However, only TNBS-modified cells were sensitive to destruction by antibody-plus-complement-mediated cytotoxicity.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface; D001557:Benzenesulfonates / Q000276:immunology*; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006241:Haptens; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid / Q000276:immunology*
D000954:Antigens, Surface; D001557:Benzenesulfonates; D000939:Epitopes; D006241:Haptens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; C025388:2,6-dinitro-4-trifluoromethylbenzenesulfonic acid; D014302:Trinitrobenzenesulfonic Acid