[Effect of antibody to rat alpha-fetoprotein (AFP) on protein and DNA synthesis of rat ascites hepatoma AH66 cells].
Effects of horse antibody (purified IgG) to rat AFP on synthesis with both protein and DNA of rat ascites hepatoma cells were examined. The addition of antibody to the cultures showed a decrease in protein synthesis within several hours; whereas, it showed a transient increase in the incorporation of thymidine into tumor cells approximately in 6-12 hours both in vitro and in vivo. Eventually within 24-48 hours, more than 50% of the cells in culture were killed and inhibited the synthesis of DNA by the antibody treatment. The stimulatory effect of antibody on the synthesis of DNA in the tumor cells was more obviously observed in synchronized cells which were capable to obtain by the treatment with thymidine followed by hydroxyurea. The synchronized tumor cells cultivated with antibody containing medium also exhibited the shortening of the cell cycle from 22 to 12-20 hours. Based on these effects of antibody on tumor cell growth, sequential administration of antibody followed by an anticancer drug (carboquone) was proven to be the most effective immunochemotherapeutic schedule for tumor bearing rats, showing 25-33% long-term survival in comparison with any other controls.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000920:Antibody-Dependent Cell Cytotoxicity; D002225:Carbazilquinone / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006736:Horses / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000276:immunology* / Q000378:metabolism
D000596:Amino Acids; D000906:Antibodies; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D000509:alpha-Fetoproteins; D002225:Carbazilquinone; D013936:Thymidine