[Isolation of purified glycoproteins of influenza virus and analysis of their antigenic and immunogenic activity].
Investigations carried out by the authors have demonstrated that a new specially synthesized nonionic detergent, MECK, can be successfully used for obtaining pure influenza virus surface antigens in preparative amounts. Hemagglutinin and neuraminidase isolated by means of MECK retain their structure and biological activity. Special attention has been devoted to the study of the immunological characteristics of the preparations thus obtained. The high immunogenicity of solubilized hemagglutinin and the complete retention of its antigenic activity have been confirmed. The simplicity of the method for the isolation of influenza virus surface antigens and their purification from internal viral proteins and the detergent make it possible to recommend the above method for the preparative isolation of external antigens.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification; D003902:Detergents; D000939:Epitopes / Q000032:analysis*; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006387:Hemagglutination, Viral; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology*; D008297:Male; D051379:Mice; D010215:Papio; D011817:Rabbits; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D003902:Detergents; D000939:Epitopes; D006023:Glycoproteins
Poluchenie ochishchennykh glikoproteidov virusa grippa i issledovanie ikh antigennoĭ i immunogennoĭ aktivnosti.