[Liquid storage of thrombocytes. Reaction to hypotonic shock and serotonin uptake for viability testing in vitro].
Platelet concentrates (PC) stored at +4 degrees C, +12 degrees C and +22 degrees C for 3 days were characterized by pH-values, hypotonic shock response (HSR) and serotonin uptake as in vitro viability criteria. PC after storage in siliconized glass bottles at +22 degrees C maintained their neutral pH-values, their ability to HSR and normal serotonin uptake up to 3 days, if their average platelet number amounted to 1,000 X 10(9) X 1(-1). PC stored at +12 degrees C with an average of 1,900 X 10(9) platelets X 1(-1) showed a comparably good vitality, which was significantly better maintained than at +4 degrees C, but only on the first day. At both temperatures, +12 degrees C and +4 degrees C, HSR and serotonin uptake declined to less than 60% after 3 days. Different strong linear relations between HSR and serotonin uptake at the 3 storage temperatures should be concluded from the calculated correlation coefficients. An influence of adenine and guanosine added to the ACD-solution on stored platelets could not be found with HSR and serotonin uptake measurements.
D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D002951:Citrates; D019343:Citric Acid; D005079:Excipients; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010976:Platelet Count; D012701:Serotonin / Q000097:blood*; D013696:Temperature
D002951:Citrates; D005079:Excipients; D019343:Citric Acid; D012701:Serotonin
Zur Thrombozytenflüssiglagerung. Reaktion auf hypotonen Schock und Serotoninaufnahme zur in vitro Vitalitätstestung.