Comparison of allogeneic and xenogeneic determinants on the H-2Kk molecule.
Two monoclonal rat anti-H-2 antibodies were derived by cell fusion from a rat immunized with mouse CH-1 lymphoma cells. Both antibodies recognize polymorphic H-2 determinants expressed on most haplotypes. Competitive binding studies on H-2Kk show that one rat antibody, R1-9.6, detects a determinant that is distant from the allodeterminants defined by 12 different mouse monoclonal anti H-2 antibodies. In contrast, the rat antibody R1-21.2 inhibits the binding of most mouse monoclonal anti H-2 antibodies to H-2k cells, possibly by causing a conformational change in the H-2 molecule. Both antibodies bind strongly to H-2 molecules and can be used for structural and biochemical studies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D007518:Isoantibodies / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D006183:H-2 Antigens; D007518:Isoantibodies