Histamine release induced by dynorphin-(1-13) from rat mast cells.
Dynorphin is a potent opioid peptide. A synthetic dynorphin-(1-13) (Dyn) induced histamine release accompanied by degranulation from isolated rat mast cells in a dose-dependent manner over the concentration range 10(-7)-10(-5) M. Dynorphin-(1-13)-induced histamine release completed within 10 sec at 37 degrees C, and the release was not accompanied by the leakage of lactate dehydrogenase. Calcium (10(-5)-10(-3) M) enhanced the release, although higher concentration than 10(-3) M suppressed the release. The pH for the maximum release by Dyn was about 7.3. Disodium cromoglycate (5 X 10(-6)-10(-4) M) inhibited the histamine release by Dyn, but naloxone and leucine-enkephalin did not. These results indicate that Dyn-induced histamine release was not mediated by opioid receptors of the mast cells. Its mode of action appeared to that of anaphylactic histamine release.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D004399:Dynorphins / Q000494:pharmacology*; D004743:Enkephalin, Leucine / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D013997:Time Factors
D010446:Peptide Fragments; D009270:Naloxone; D004743:Enkephalin, Leucine; C026843:dynorphin (1-13); D004399:Dynorphins; D007770:L-Lactate Dehydrogenase; D002122:Calcium Chloride; D004205:Cromolyn Sodium