Idiotype-specific T lymphocytes. II. capability of generation of idiotype-specific T lymphocytes is determined by corresponding VH gene.
MOPC-104E (M104E) idiotype-specific and major histocompatibility complex-restricted T lymphocyte activities were investigated in BALB/c (Igh-1a) and its Igh-1-congenic strains of mice, such as C.AL-20 (Igh-1d), BAB-14 (Igh-1b), and CB-20 (Igh-1b). Idiotype-specific T lymphocytes could be induced in BALB/c and BAB-14 by immunization of viable M104E tumor cells followed by surgical removal and in vitro restimulation with the homologous tumor cells. On the other hand, C.AL-20 and CB-20 mice did not show the idiotype-recognizing capacity even though they could mount comparable cytotoxic T lymphocyte activities against M104E to BALB/c and BAB-14. The results strongly suggest that the inducibility of M104E cross-reactive idiotypy closely paralleled the producibility of corresponding idiotype-specific T lymphocytes. Genetically defined VH gene products might act as internal images that construct idiotype network systems.
D000818:Animals; D000917:Antibody Formation; D001666:Binding Sites, Antibody / Q000235:genetics*; D003429:Cross Reactions; D003911:Dextrans / Q000276:immunology; D005260:Female; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000235:genetics* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*
D003911:Dextrans; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region