Immunological relationship between the EDTA-extractable proteins from calf lens fiber membranes.
An antiserum has been prepared against the EDTA-extractable proteins (EEP) from calf lens fiber membranes. It was shown to be highly specific for EEP. Using this anti-EEP antiserum in (crossed-line) immunoelectrophoresis and (crossed) immunoelectrofocusing experiments, evidence was obtained that all of the EDTA-extractable proteins are immunologically related. They have at least one of six different antigenic determinants in common, while some of the proteins with pI values above 4.8 probably have a second common determinant. The acidic proteins of EEP comprise specific determinants with a low immunogenicity. Furthermore, it was demonstrated that no crystallin-like determinant was present on the EEP molecules.
D000818:Animals; D000918:Antibody Specificity; D001345:Autoradiography; D002417:Cattle; D003459:Crystallins / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005136:Eye Proteins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing
D003459:Crystallins; C030327:EDTA-extractable protein (lens membrane); D000939:Epitopes; D005136:Eye Proteins; D007106:Immune Sera