title: The amount of white pulp in the spleen; a morphometrical study done in methacrylate-embedded splenectomy specimens.
abstract: This report deals with a morphometrical study on 92 surgically removed human spleens, to investigate the composition of spleens which are considered to be normal, i.e. spleens which had ruptured in traffic accidents or which had been incidentally removed during abdominal operations. A comparison was made with 16 spleens with hypersequestration of platelets and to 11 with hyper-sequestration of erythrocytes. Methyl-methacrylate embedding was used because of the superiority of this technique over conventional paraffin embedding. Significant differences were found between both 'normal' groups as to the absolute and relative amount of white pulp as well as the perifollicular red pulp zone. Based also on the few morphometrical reported studies in the literature, spleens removed during abdominal surgery form the best control group. Traumatic rupture of the spleen in traffic accidents might specifically occur in spleens which already contained a stimulated lymphatic compartment. A probably non-specific increase of white pulp was found in splenomegaly of varied aetiology. An expected influence of age on weight and composition of the spleen was not found in our study. The spleen changes in weight and composition only up to 5 years of age. Significant involution at older age was not found in ours nor in other reported larger series.
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000741:Anemia, Aplastic / Q000473:pathology; D006801:Humans; D008768:Methylmethacrylates; D008875:Middle Aged; D009929:Organ Size; D012016:Reference Values; D013103:Spherocytosis, Hereditary / Q000473:pathology; D013154:Spleen / Q000033:anatomy & histology* / Q000293:injuries; D013156:Splenectomy; D013161:Splenic Rupture / Q000473:pathology; D013194:Staining and Labeling
chemical_list: D008768:Methylmethacrylates