Improvement in the specificity of the silver staining technique for AgNOR-associated acidic proteins in paraffin sections.
Some recently developed silver staining methods allow selective staining of acidic nucleolar proteins. Pretreating deparaffinized sections with Schiff's reagent improves the specificity of Goodpasture and Bloom's AgNOR staining (as modified by Kodama et al.) after aldehyde fixation.
D000818:Animals; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D008099:Liver / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D010232:Paraffin; D011506:Proteins / Q000032:analysis*; D051381:Rats; D012834:Silver; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds
D004396:Coloring Agents; D011506:Proteins; D013438:Sulfhydryl Compounds; D012834:Silver; D010232:Paraffin; C005732:thioacetic acid; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin