Protein synthesis in Escherichia coli during recovery from exposure to low levels of Cd2+.
Exposure of Escherichia coli to 3 microM Cd2+ results in 84 to 95% of the cells losing their ability to form colonies on plates of nutrient agar. Transfer of the cells to Cd2+-free liquid medium results in a recovery of colony-forming ability without significant synthesis of DNA. As an early event in recovery, the cells exhibit a rapid uptake of [3H]leucine. Recovery and this incorporation are inhibited by chloramphenicol or rifampin. Sodium dodecyl sulfate-gel electrophoresis of proteins from recovering cells labeled with [3H]leucine for 1 min indicated the synthesis of at least two classes of proteins with apparent molecular weights of 55,000 to 65,000. One class bound Cd2+ and was absent in untreated cultures. The other class of proteins, which did not bind Cd2+, was synthesized at a rapid rate in recovering cells and may be a normal cellular protein.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D004269:DNA, Bacterial / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008628:Mercury / Q000378:metabolism / Q000494:pharmacology; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D012329:RNA, Bacterial / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D002104:Cadmium; D012967:Sodium Dodecyl Sulfate; D002701:Chloramphenicol; D008628:Mercury; D012293:Rifampin