Factor-dependent in vitro growth of human normal bone marrow-derived basophil-like cells.
A factor(s) present in supernatants from lectin-stimulated peripheral blood mononuclear cells promoted the production of basophil-like cells in liquid cultures of normal human bone marrow cells. The cultured basophil-like cells had lobulated or round nuclei, and the cytoplasmic granules stained metachromatically with toluidine blue and azurophilic with Giemsa. 20% of the metachromatically staining cells were peroxidase positive but not positive for nonspecific esterase. The histamine content was 0.5-2 pg/cell. The basophil-like cells released histamine upon challenge with calcium ionophore A23187 but not with compound 48/80. They also released histamine with anti-IgE when passively sensitized with human myeloma IgE. The development of basophil-like cells was promoted in a dose-dependent fashion by a factor(s) in the conditioned medium. Blocking of cell proliferation with hydroxyurea or X irradiation inhibited the development of basophil-like cells. The production of the factor was dependent on the presence of T cells. The factor was different from interleukin 2 and its molecular weight was estimated to be 25,000-40,000 by gel filtration on a Sephacryl S-200 column. Thus, human basophil-like cells derived from normal bone marrow cells can grow and differentiate in vitro under the regulation of T cells.
D001491:Basophils / Q000166:cytology*; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D055598:Chemical Phenomena; D002621:Chemistry; D003208:Concanavalin A / Q000502:physiology*; D003470:Culture Media; D003594:Cytoplasmic Granules / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D008222:Lymphokines; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology
D003470:Culture Media; D007376:Interleukin-2; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A