Isolated fractions of the growth-promoting alpha-globulin stimulate differently the incorporation of exogenous DNA into L cells.
The growth-promoting alpha-globulin necessary for long-term cultivation of mammalian cells in serum-free medium is a complex of several proteins which also stimulates the incorporation of exogenous DNA into recipient L cells. Biological activity responsible for the stimulation of DNA incorporation into L cells can be concentrated because one of two major protein components, compared to the whole GPAG complex, increases incorporation into cell nuclei by 100%.
D000510:Alpha-Globulins / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D007700:Kinetics; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D014316:Tritium
D000510:Alpha-Globulins; D014316:Tritium; D004247:DNA