Anti-idiotypes against anti-H-2 antibodies VI. Detection of shared idiotypes among monoclonal anti-H-2 antibodies.
Anti-idiotypes produced against monoclonal anti-H-2 antibodies have been used to examine idiotope sharing among a panel of anti-major histocompatibility complex (MHC) antibodies detecting the same and different specificities. Both xenogeneic anti-100-30 and anti-3-83, which did not react with predominant idiotypes in conventional alloantisera, detected idiotopes on 3-83 and 100-30 as well as on 2 other monoclonal anti-H-2Kk antibodies. All 4 monoclonal antibodies recognized the same epitope cluster on the Kk molecule and detected the same serological specificity. H-2.5. In contrast, syngeneic and allogeneic anti-idiotype produced against the same monoclonal antibodies did not detect these cross-reactive determinants. In several instances, xenogeneic anti-idiotype reacted with anti-MHC antibodies which recognized distinct H-2 determinants, suggesting that anti-MHC antibodies detecting the same or different specificities may share idiotypic determinants. These reagents may be useful probes of the anti-MHC immune repertoire.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007518:Isoantibodies / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011817:Rabbits; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies; C030036:anti-H-2 antibodies