Cytochemical demonstration of adenylate cyclase with strontium chloride in the rat pancreas.
A cytochemical procedure for the localization of adenylate cyclase with Sr2+ as the capture ion and adenylyl imidodiphosphate as the specific substrate was evaluated in the rat pancreas. Incubation medium was unaffected by the addition of 5 mM strontium ions but became turbid in the presence of lead or strontium plus 10 mM NaF. Tissues were prefixed in 2% formaldehyde/0.5% glutaraldehyde and incubated, and the cytochemical precipitate was converted to the Pb2+ salt. Enzymatic activity was demonstrated on the plasma membrane of pancreatic acinar cells and responded to stimulation by secretin. Controls frequently contained Pb2+ sequestered in mitochondria, but otherwise only a few randomly distributed grains were observed. The controls were 1) omission of substrate from the medium; 2) incubation of tissue for 1 min in complete medium; and 3) tissue previously inactivated by microwave irradiation and incubated for 30 min in complete medium including secretin.
D000262:Adenylyl Cyclases / Q000032:analysis*; D000266:Adenylyl Imidodiphosphate; D000818:Animals; D006651:Histocytochemistry / Q000379:methods; D007854:Lead; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D013194:Staining and Labeling; D013324:Strontium
D000266:Adenylyl Imidodiphosphate; D007854:Lead; D000262:Adenylyl Cyclases; C025700:strontium chloride; D013324:Strontium