Electrophoretic elution of nucleic acids from acrylamide and agarose gels.
A simple method for electrophoretic elution of nucleic acids from gel slices is described. The procedure utilizes a standard tube gel system and can be completed in as little as one hour. Nucleic acids are recovered in a small volume with almost 100% efficiency. The procedure is applicable equally to acrylamide and agarose gels, and small as well as large RNA and DNA molecules. The eluted nucleic acids are essentially undegraded and are suitable for a variety of structural and biological analyses.
D004247:DNA / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D012313:RNA / Q000032:analysis
D009696:Nucleic Acids; D012313:RNA; D004247:DNA