High-performance liquid chromatography of proteins: purification of alpha-fetoprotein from fetal calf serum.
High-performance liquid chromatography was utilized for the purification of bovine alpha-fetoprotein (BAFP) from fetal calf serum (FCS). An initial step in the purification involved absorption of charcoal delipidated FCS on Cibacron Blue F3GA gel. The Cibacron Blue pre-purified FCS was then chromatographed on a Polyanion SI weak anion-exchange column. The BAFP isolated had a purity of greater than 93% with an overall yield of 48% from FCS. The procedure was applicable for semi-preparative scale purification of BAFP.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D014227:Triazines; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D001798:Blood Proteins; D014227:Triazines; D000509:alpha-Fetoproteins; D012710:Serum Albumin, Bovine; C012842:Cibacron Blue F 3GA