Monoclonal antibodies recognizing structural components of murine retroviruses including an FMR antigen on protein p12.
Monoclonal antibodies were prepared from mice and rats immunized with Friend leukaemia virus and BALB/c xenotropic virus. By immunoprecipitation of 125I-labelled and [35S]methionine-labelled viruses and by protein blotting, ten antibodies were found to react with the viral components p12, p15, p30, gp70 and p15E/p12E. A dot-immunobinding assay was found to be a reliable method to type the antibody reactivity with different murine leukaemia viruses (MuLVs). When tested on a panel of ecotropic and xenotropic MuLVs the antibodies revealed the following antigenic specificities: ecotrop-specific on p15E/p12E; xenotrop-specific on p15E; group-specific on p30 and p15E; FM-specific on gp70; FR-specific on gp70 and p15. Of particular interest is a cytotoxic antibody recognizing an FMR determinant localized on p12.
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005622:Friend murine leukemia virus / Q000276:immunology*; D015683:Gene Products, gag; D006825:Hybridomas / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; C030663:FMR antigen; D015683:Gene Products, gag; D014764:Viral Proteins