title: Cytoplasmic RNA from normal and malignant human cells shows homology to the DNAs of Epstein-Barr virus and human adenoviruses.
abstract: Cytoplasmic RNA prepared from several human cell lines and tissues was hybridised to DNA from Epstein-Barr virus, human adenovirus types 2, 3 and 12 and human papovaviruses BK and JC. RNA from all the cells, regardless of whether or not they were virally infected, hybridised to specific regions of the Epstein-Barr virus or adenovirus genomes but not to papovavirus DNA. The cellular cross-hybridising species appear to be repetitive sequences which are conserved in higher eukaryotes. Mismatch estimations indicate a high degree of homology between the viral and host sequences. Detailed analysis of selected regions of viral DNA failed to reveal any primary-structural peculiarities.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D001402:B-Lymphocytes / Q000032:analysis; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000032:analysis; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D004279:DNA, Viral; D012334:RNA, Neoplasm; D012313:RNA; D004262:DNA Restriction Enzymes