[Determination of alpha-1-fetoprotein in maternal serum dried on filter paper by an enzyme immunoassay].
The suitability of different carrier materials for absorption of serum was investigated with respect to a centralised screening for AFP serum levels in pregnant women, which needs a transmission of the samples. AFP was quantified in an indirect two-site binding enzyme immunoassay. AFP concentrations of native serum and of the eluate from the samples dried on filter paper were in a good agreement with a coefficient of correlation of 0.902. But storage of dried serum samples should not exceed one week because a two week storage diminished the coefficient of correlation to 0.766 and AFP concentrations of paper dried samples were overestimated.
D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins
Bestimmung von Alpha-1-Fetoprotein in auf Filterpapier angetrocknetem Schwangerenserum mit einem Enzymimmunoassay.