The use of particle counting immunoassay (PACIA) for the titration of specific antibodies. Application to sera from honeybee venom-desensitized patients.
Particle counting immunoassay (PACIA) is shown to be a suitable nonradioisotopic method for the titration of anti-phospholipase A2 antibodies in the sera from honeybee venom-desensitized patients. The sensitivity of the method is equal to the RAST-IgG with a correlation coefficient between the two methods of r = 0.94. Within-day and between-day precisions were 2.5 and 9.2%, respectively. The main advantages of PACIA are: (i) the use of easily prepared non-radioactive reagents having a shelf-life of at least 1 year at -20 degrees C: (ii) partial automation; (iii) calibration with antisera of animal origin, and (iv) the possibility of allergen standardization of honeybee venom extracts.
D000371:Agglutination; D000485:Allergens / Q000592:standards; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001514:Bee Venoms / Q000008:administration & dosage; D001667:Binding, Competitive; D003888:Desensitization, Immunologic; D000939:Epitopes; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007231:Infant, Newborn; D007299:Insect Bites and Stings / Q000628:therapy*; D008863:Microspheres; D010740:Phospholipases / Q000276:immunology*; D010741:Phospholipases A / Q000276:immunology*; D011817:Rabbits; D011852:Radioallergosorbent Test
D000485:Allergens; D000906:Antibodies; D001514:Bee Venoms; D000939:Epitopes; D010740:Phospholipases; D010741:Phospholipases A