A simple method for purification of retroviruses using polyacrylnitrile.
A simple method has been developed that allows the concentration and partial purification of retroviruses from both small and large volumes of culture fluid in a comparatively short period of time. The method is based on the property of polyacrylnitrile (PAN) to adsorb preferentially nonviral proteins. Retroviruses purified by this procedure exhibit rather high specific reverse transcriptase activities and a good appearance in electron micrographs and protein patterns.
D000180:Acrylic Resins; D000327:Adsorption; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D011506:Proteins; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure
D000180:Acrylic Resins; D011506:Proteins; C010504:polyacrylonitrile; D012194:RNA-Directed DNA Polymerase