Synthesis of alpha 1-microglobulin by guinea-pig liver.
Explants from perfused guinea-pig livers were found to release alpha 1-microglobulin into the culture medium. Tritiated leucine in the medium was incorporated into the protein, suggesting a de novo synthesis of alpha 1-microglobulin by the liver tissue. The size of the protein could not be distinguished from that of purified urinary alpha 1-microglobulin when tested with sodium dodecyl sulfate/polyacrylamide gel electrophoresis. After intravenous injections of tritiated leucine into guinea-pigs, the 105 000 X g pellet of homogenized liver rapidly increased its content of radioactive alpha 1-microglobulin, with a maximum after 20 min. 3H-Labelled alpha 1-microglobulin appeared in serum after a lag phase of 20 min, and by comparing the rate of accumulation with albumin, the synthesis of guinea-pig alpha 1-microglobulin could be estimated to 20 micrograms g liver-1 h-1.
D000510:Alpha-Globulins / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D006168:Guinea Pigs; D007120:Immunochemistry; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism*
D000510:Alpha-Globulins; D003470:Culture Media; C016001:alpha-1-microglobulin; D007930:Leucine