Interferon gamma-induced Ca-dependent protein kinase in mouse L cells.
Treatment of mouse L cells with mouse IFN gamma induced a cytoplasmic Ca-dependent protein kinase, which highly phosphorylated cellular enzymes such as phosphodiesterase and RNase in vitro. The kinase partially purified from IFN gamma-treated cells (100 units/ml, 12 h at 37 degrees C) was different from IFN-induced dsRNA-dependent protein kinase since it was dsRNA independent. The kinase may have played an important role in mediating IFN-induced biological effects, since cellular enzymes were found to alter enzyme activity after phosphorylation by the kinase in vitro.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007739:L Cells / Q000201:enzymology*; D051379:Mice; D011494:Protein Kinases / Q000378:metabolism*; D012313:RNA / Q000378:metabolism
D003609:Dactinomycin; D012313:RNA; D007371:Interferon-gamma; D011494:Protein Kinases; D002118:Calcium