Idiotype-specific T-helper cells.
In the present study we investigated the induction and fine specificity of T-helper cells that recognize idiotypes. The data presented show that both low-dose priming with anti-T15 antiserum and priming with PC-Hy are effective in stimulating T15-specific T help. Phosphorylcholine-hemocyanin priming can generate these T cells in either PC-responding or nonresponding strains of mice. Furthermore, the PC-primed T-helper cells can also recognize another anti-PC myeloma, M167, that is idiotypically different from T15. The fine specificity of the anti-PC-idiotype recognizing T-helper cells was examined by studying the effect of in vitro inhibitors on the T-cell help. Both PC and PC-BSA as well as T15 and M167 had an inhibitory effect on the T help. Free T15 and M167 heavy chains also blocked the helper activity for T15; T15 and M167 light chains had no effect, however. Viewed collectively, these results show that PC-Hy priming induces T-helper cells that recognize idiotypic determinants common to both T15 and M167, and that the proteins' H chain is the major structural component of the determinant. Finally, the generation of these idiotype-recognizing T cells was found to occur by way of a T-T interaction loop, based on the finding that T-helper cells are induced by PC-Hy priming in animals that lack PC-responding B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009194:Myeloma Proteins / Q000276:immunology; D010767:Phosphorylcholine / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins; D014301:Trinitrobenzenes; D010767:Phosphorylcholine; D006433:Hemocyanins; D002794:Choline