Determination of monoamines in brain nuclei by high performance liquid chromatography with electrochemical detection: young vs. middle aged rats.
A previously described high performance liquid chromatographic method for simultaneously measuring norepinephrine, dopamine, serotonin and 5-hydroxyindole acetic acid has been modified to permit analysis of the monoamines in microdissected brain nuclei from a single rat. Results of measurements in 12 nuclei of young and middle aged male rats are presented.
D000375:Aging; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis
D001679:Biogenic Amines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D009638:Norepinephrine