Synthesis of active firefly luciferase by in vitro translation of RNA obtained from adult lanterns.
Poly (A)+ RNA was isolated from the lanterns of adult fireflies, Photinus pyralis. The Poly (A)+ RNA was translated in a cell-free translation mixture from rabbit reticulocytes and the synthesis of enzymatically active firefly luciferase was demonstrated. The translation products were immunoprecipitated with anti-luciferase and then subjected to SDS gel electrophoresis. It was shown that a newly synthesized polypeptide exhibited an identical electrophoretic mobility as the purified enzyme.
D000818:Animals; D002474:Cell-Free System; D001517:Coleoptera / Q000201:enzymology*; D007700:Kinetics; D008156:Luciferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D008156:Luciferases