A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2-blood-group chains.
A monoclonal antibody C14/1/46/10 showing preferential binding to membranes of human colorectal carcinomas over normal colon mucosae was obtained by immunization of mice with extra-nuclear membranes of a human colonic adenoma. Binding and inhibition of binding assays using blood cells or glycoproteins with known blood-group activities indicated that the antibody recognizes a carbohydrate antigen co-existing with the blood-group-H determinant: Fuc alpha 1 leads to 2 Gal. Inhibition assays with structurally defined oligosaccharides showed that the antigenic determinant involves difucosylated Type-2-blood-group chains with the structure: (formula; see text)
D000236:Adenoma / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000912:Antibodies, Neoplasm; D001666:Binding Sites, Antibody; D001789:Blood Group Antigens / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology*; D004285:Dogs; D000939:Epitopes; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D001789:Blood Group Antigens; D000939:Epitopes