Allergen- and anti-IgE-induced histamine release from whole blood.
The release of histamine by allergen and anti-IgE from whole blood was observed in 34 asthmatic subjects with a positive skin test to house dust. The time course of histamine release showed that the release by allergen and anti-IgE peaked after 15 min incubation. There was no significant difference in the time course of the release from whole blood between allergen and anti-IgE. Anti-IgE-induced histamine release correlated to a certain extent with the serum IgE level. Histamine release by house dust, on the other hand, correlated with the radioallergosorbent test score. A striking difference was present in the dose-response slope between allergen and anti-IgE. The maximum percent release correlated with the dose-response slope by allergen, but not by anti-IgE. The amount of histamine release induced by anti-IgE paralleled the amount of the release by house dust in the cases sensitive to the allergen; less sensitive basophils to anti-IgE were less sensitive to house dust.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000918:Antibody Specificity; D001491:Basophils / Q000276:immunology; D001769:Blood; D004391:Dust; D005260:Female; D006636:Histamine Release; D006798:Housing; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D007073:Immunoglobulin E / Q000276:immunology*; D007700:Kinetics; D008297:Male; D008875:Middle Aged
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D004391:Dust; C026408:anti-IgE antibodies; D007073:Immunoglobulin E