Enrichment of IL-2-producer T cells from mouse spleen by use of Bauhinia purpurea lectin.
Bauhinia purpurea agglutinin (BpA) was used to enrich the interleukin 2 (IL 2)-producing T-cell subset from mouse spleen. IL 2 was found to be produced by the BpA-non-agglutinated (BpA-) T cells of mouse spleen on stimulation with concanavalin A (Con A). The degree of IL 2 production by BpA-agglutinated (BpA+) T cells was significantly low. The proliferative response to Con A of BpA- T cells was not influenced by the addition of exogenous IL 2, whereas BpA+ T cells were partially dependent on the exogenous addition of IL 2 in the proliferative response.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis*; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010641:Phenotype; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D011975:Receptors, Mitogen; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
C039340:Bauhinia purpurea lectin; D007376:Interleukin-2; D037102:Lectins; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell; D011975:Receptors, Mitogen; D003208:Concanavalin A