Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.
Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome. Southern hybridisation analysis of DNA isolated from transformants revealed the presence of pBR322 sequences which have integrated into the host genome along with the N. crassa DNA.
D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics*; D005798:Genes, Bacterial; D005815:Genetic Code; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D014170:Transformation, Genetic
D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase