Intercellular NOR-Ag variability in man. I. Technical improvements and marker acrocentric chromosomes.
A new modification of the Ag I technique has been developed using human cultured blood lymphocytes, which involves ultra-violet irradiation of chromosome preparations during incubation in AgNO3. This technique enables detection in a short incubation time all NORs capable of being stained with silver. A peculiar morphological change in Ag-stainable NORs during the incubation is described, which can be used as a criterion of the completion of Ag staining. With the refined Ag-staining procedure, acrocentric marker chromosomes were studied which showed one or two satellite stalks within the same individual. Ag staining was highly coincident with this variability.
D002901:Chromosomes, Human, 13-15; D002904:Chromosomes, Human, 21-22 and Y; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012835:Silver Nitrate; D013194:Staining and Labeling
D005819:Genetic Markers; D012835:Silver Nitrate