Studies on the carbohydrate-protein linkage region in bovine corneal keratan sulfate. III. Evidence for a biantennary structure.
Peptidokeratan sulfates were retained by a concanavalin A-Sepharose column and readily eluted with 20 mM methyl alpha-D-mannoside from the column. Linkage region-enriched glycopeptides, prepared by endo-beta-galactosidase digestion of the peptidokeratan sulfates, behaved similarly to the peptidokeratan sulfates on the same affinity chromatography. Further, behaviors of both the peptidokeratan sulfates and the linkage region-enriched glycopeptides on pea lectin-Agarose columns were consistent with the results of the chromatography on concanavalin A-Sepharose. These findings confirmed the typical biantennary structure previously suggested for the linkage region on the basis of structural studies of the linkage region-enriched glycopeptides.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D003315:Cornea / Q000032:analysis*; D005696:Galactosidases / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis; D006025:Glycosaminoglycans; D007632:Keratan Sulfate; D008970:Molecular Weight; D011485:Protein Binding
D002241:Carbohydrates; D006020:Glycopeptides; D006025:Glycosaminoglycans; D007632:Keratan Sulfate; D005696:Galactosidases