title: Specificity of the T cells that mediate and suppress adoptive immunotherapy of established tumors.
abstract: This study was designed to investigate the specificity of the T cells that express and suppress antitumor immunity in a model of adoptive immunization against established tumors. Results obtained with the P815 mastocytoma, L5178Y lymphoma, and P388 lymphoma showed, in agreement with previous findings from this laboratory, that an intravenous infusion of splenic T cells from immunized mice can cause the regression of a tumor growing in T-cell-deficient mice. So far as specificity of adoptive immunity is concerned, reciprocal passive transfer experiments with these three tumors revealed that T cells from donor mice immunized against the P815 tumor are not capable of causing regression of the P388 tumor or L5178Y tumor, even if both the P815 and L5178Y tumors are growing in the same host. Similarly splenic T cells from mice immunized against the P388 tumor or L5178Y tumor had no effect on growth of the P815 tumor. Suppression of adoptive immunity was also specific, in that passively transferred suppressor T cells from mice bearing a progressive P815 tumor were capable of suppressing adoptive T-cell-mediated regression of the P815 tumor, but not the P388 tumor growing in T-cell-deficient recipients. Reciprocally, P388 suppressor spleen cells from mice bearing a progressive P388 tumor prevented adoptive T-cell-mediated regression of the P388 tumor, but not the P815 tumor. The results indicate, therefore, that the T cells from immunized mice that mediate adoptive antitumor immunity and the T cells from tumor-bearing mice that suppress the expression of this immunity are specific for the tumor that evokes their generation.
mesh_terms: D000818:Animals; D002460:Cell Line; D007116:Immunization, Passive; D007167:Immunotherapy; D007940:Leukemia L5178 / Q000276:immunology* / Q000628:therapy*; D007941:Leukemia P388 / Q000276:immunology* / Q000628:therapy*; D007942:Leukemia, Experimental / Q000276:immunology* / Q000628:therapy*; D012515:Mast-Cell Sarcoma / Q000276:immunology* / Q000628:therapy*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology