Human gingival fibroblast production of interferon.
Three human gingival fibroblast cell lines were used to determine whether they could be induced by a synthetic RNA and superinduced by metabolic inhibitors to produce interferon (IFN-beta). When established procedures were followed for human fetal or newborn skin fibroblast cell lines, the adult gingival fibroblasts produced comparable amounts of IFN-beta. It was shown that the superinducers alone would not cause an IFN-beta production response, and that the absence of serum in the production medium also inhibited the production of IFN-beta. The effect of IFN-beta on cell growth was carried out in T-flasks seeded with 10(5) HEp-2 cells. After one and two wk, the cells of triplicate control flasks and triplicate flasks containing various dilutions of the production media were enumerated to determine a cell multiplication inhibition (CMI) value. A correlation between the IFN-beta content and the CMI effect, however, was not obtained, and it was concluded that other CMI agents, possibly more potent than the IFN-beta, were being produced by the stimulated human gingival fibroblasts. Cell protein assays which gave a high ng/cell protein content correlated with TEM micrographs which showed clusters of complex lysosomes, primarily in cells cultured in the IFN-beta-containing nutrient. However, since commercial IFN-beta initiated no such lysosomal response, it was further concluded that the complex lysosomes were due to CMI agent(s) other than IFN-beta.
D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000648:ultrastructure; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis* / Q000494:pharmacology; D009062:Mouth Neoplasms / Q000473:pathology / Q000648:ultrastructure
D003470:Culture Media; D007369:Interferon Inducers; D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide