A rho-independent termination caused by the cloned inverted nut L site of phage lambda.
For studying the termination activity of inverted nutL site of bacteriophage lambda, we have constructed a plasmid carrying the nutL fragment oriented reversely with respect to cloned lambda promoter p'R-directed transcription. The results of in vitro transcription on this plasmid template and S1 mapping assay reveal that the termination of p'R-promoted transcription at inverted nutL site is a rho-independent event. This nutL terminator shares several features with the other known sites of transcription termination, including (i) a uridine-rich 3' terminal RNA sequences,--UUAAUUUUU-OH, (ii) a GC-rich region in the DNA immediately preceding the site of termination, (iii) a region of dyad symmetry in the DNA which, in transcript, is capable of forming a stable hairpin containing four GC base pairs and one AU base pair in its stem.
D010582:Bacteriophage lambda / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012234:Rho Factor / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004279:DNA, Viral; D012333:RNA, Messenger; D012234:Rho Factor; D014157:Transcription Factors