Nicotinamide and other inhibitors of ADP-ribosylation block deoxyglucose uptake in cultured cells.
Nicotinamide was shown to inhibit deoxyglucose uptake in three diverse differentiated cell lines. In 3T3-L1 fat cells, nicotinamide equally inhibited basal and insulin stimulated deoxyglucose uptake. Inhibition by nicotinamide was non-competitive. A variety of inhibitors of ADP-ribosylation blocked deoxyglucose uptake while some analogs with no activity against ADP-ribose synthetase also had little effect on deoxyglucose uptake. These findings should be taken into account when inhibitors of ADP-ribosylation are used with intact cells.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001842:Bone and Bones / Q000378:metabolism; D002460:Cell Line; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D051379:Mice; D009525:Niacin / Q000494:pharmacology; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011736:Pyridoxine / Q000494:pharmacology; D011972:Receptor, Insulin / Q000276:immunology
D000906:Antibodies; D003837:Deoxy Sugars; D007328:Insulin; D009702:Nucleoside Diphosphate Sugars; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; D009525:Niacin; D003847:Deoxyglucose; D011972:Receptor, Insulin; D011736:Pyridoxine; C024058:N(1)-methylnicotinamide; C008472:N-methylnicotinamide