A new technique for the preparation of ready-to-use macroaggregated albumin (MAA) kits to be labelled with 99mTc for lung scanning.
Kits were developed for the sterile labelling of macroaggregated albumin (human) with 99mTc. 0.4 mL of 20% HSA, 1 mL of SnCl2 X 2H2O containing 5 mg and 1.1 mL of 0.9% NaCl were sonified for 10 min. The pH was adjusted with phosphate buffer to 5.5 after the addition of 1 mL of 5% Tween-80. The solution was heated with stirring for 10 min at 80 degrees C for aggregation and 1.0 mL portions were placed in 10 mL vials for lyophilization. The contents of single reaction vials were reacted with 99mTc and the radiochemical yield determined (higher than 98%). The MAA kits were stable and the stability of [99mTc]-MAA was followed for 4.5 h. Lung uptake in mice was determined to be about 98.5%. The preparation of [99mTc]-MAA was performed in a single step process and excellent human lung scans were obtained.
D000818:Animals; D004355:Drug Stability; D006801:Humans; D008168:Lung / Q000000981:diagnostic imaging*; D051379:Mice; D010316:Particle Size; D011877:Radionuclide Imaging; D012709:Serum Albumin / Q000378:metabolism; D013667:Technetium / Q000378:metabolism; D013668:Technetium Tc 99m Aggregated Albumin; D014018:Tissue Distribution
D012709:Serum Albumin; D013668:Technetium Tc 99m Aggregated Albumin; D013667:Technetium