Energy production and utilization in contractile failure due to intracellular calcium overload.
Intracellular calcium overload was produced by perfusing the Ca2+- deprived rat hearts for 5 or 10 min with normal medium containing 1.25 mM Ca2+ for 10 min and changes in the myofibrillar and membrane ATPase, sarcolemmal adenylate cyclase, mitochondrial oxidative phosphorylation and high energy phosphate stores in failing hearts were examined. Myocardial creatine phosphate and ATP were decreased by the intracellular calcium overload whereas the myofibrillar, mitochondrial and microsomal ATPase activities were not altered. The intracellular calcium overload markedly depressed the mitochondrial oxidative phosphorylation as well as sarcolemmal Ca2+ ATPase, Mg2+ ATPase, Na+ - K+ ATPase and adenylate cyclase. These results suggest that abnormalities in the process of energy production rather than energy utilization may primarily account for the depressed energy state of hearts failing due to an intracellular calcium overload.
D000244:Adenosine Diphosphate / Q000032:analysis; D000249:Adenosine Monophosphate / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004734:Energy Metabolism; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010477:Perfusion; D010725:Phosphocreatine / Q000032:analysis; D051381:Rats; D012508:Sarcolemma / Q000201:enzymology
D010725:Phosphocreatine; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D002118:Calcium