Endogenous calmodulin and Ca2+-ATPase activity of human erythrocyte membranes.
Treatment of calmodulin-depleted erythrocyte membranes with oleic acid solubilizes only a minor fraction of membrane proteins which, however, contains more than 90% of the total endogenous calmodulin. Concomitantly, Ca2+-ATPase is completely and irreversibly inactivated. By treatment with oleic acid in the presence of Ca.ATP the inactivation of Ca2+-ATPase is abolished to a great extent, the loss of endogenous calmodulin, however, is not affected. Thus, a major participation of endogenous calmodulin in the regulation of Ca2+-ATPase of erythrocyte membranes seems to be improbable.
D000252:Calcium-Transporting ATPases / Q000097:blood*; D002147:Calmodulin / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology
D002147:Calmodulin; D009829:Oleic Acids; D019301:Oleic Acid; D000252:Calcium-Transporting ATPases