Regulation of naturally occurring autoantibody secretion by a radiosensitive lymphocyte: initial characterization and ontogeny.
Low level irradiation (400-500 R) of normal mice or of murine spleen cells resulted in the detection of an enhanced number of plaque-forming cells against bromelain-treated autologous red cells (Br MRBC) 1 day later. The mechanism responsible for the increased numbers of plaques is apparently the elimination of a suppressor T cell since the addition of thymocytes or of Lyt 1+2+ splenic cells to cultures of irradiated cells reversed the radiation-induced increase. Studies on the ontogeny of the phenomenon indicate that anti-Br MRBC plaques can be formed by spleen cells taken from mice shortly after birth although adult levels are not reached until after 3 weeks of age. Radiation-induced increases in the number of plaques were not seen until 3 weeks of age, thus, suggesting a temporal developmental sequence of the ability to produce autoantibodies and to regulate such production.
D000375:Aging; D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D001323:Autoantibodies / Q000096:biosynthesis*; D001963:Bromelains / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006462:Hemolytic Plaque Technique; D051379:Mice; D008809:Mice, Inbred C3H; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects
D000950:Antigens, Ly; D001323:Autoantibodies; D001963:Bromelains