A distinct beta-hexosaminidase isoenzyme separated from human leukemic lymphocytes and myelocytes.
The beta-hexosaminidase (EC 3.2.1.30) isoenzymes were separated on the basis of their carbohydrate moieties by an affinity chromatography using immobilized phenylboronate. Normal lymphocytes and granulocytes contain two major forms of beta-hexosaminidase, acute lymphoblastic, acute myeloblastic, chronic lymphocytic and chronic myelocytic leukemic cells contain an extra, distinct isoenzyme of beta-hexosaminidase. This extra isoenzyme may be a marker for the leukemic conversion of hematopoietic tissue.
D001853:Bone Marrow / Q000201:enzymology; D006596:Hexosaminidases / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D007938:Leukemia / Q000201:enzymology*; D007945:Leukemia, Lymphoid / Q000201:enzymology; D007951:Leukemia, Myeloid / Q000201:enzymology; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D009504:Neutrophils / Q000201:enzymology*; D012016:Reference Values; D001619:beta-N-Acetylhexosaminidases
D007527:Isoenzymes; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases