Inhibition of lymphoid cell DNA synthesis by metal allergens at various concentrations. Effect on short-time cultured nonadherent cells compared to nonseparated cells.
Metal allergen concentrations inhibiting lymphoid cell DNA synthesis were tested on short-time cultured nonadherent and nonseparated thymic and splenic cells. With all tested metal allergens, the DNA-synthesis-inhibiting effect was more marked for nonadherent compared to nonseparated splenic cells. In thymocytes, a stronger inhibition of nonadherent cells was seen after addition of mercuric chloride. A protective or modifying role of adherent cells towards inhibiting or toxic effects of metal allergens in vitro is postulated.
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D003035:Cobalt / Q000276:immunology; D004247:DNA / Q000096:biosynthesis*; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008627:Mercuric Chloride; D008628:Mercury / Q000276:immunology; D009532:Nickel / Q000276:immunology; D011192:Potassium Dichromate / Q000276:immunology
D000485:Allergens; D003035:Cobalt; C029938:nickel sulfate; D008627:Mercuric Chloride; D009532:Nickel; D004247:DNA; C018021:cobaltous chloride; D008628:Mercury; D011192:Potassium Dichromate