New polyenic antibiotics active against gram-positive and -negative bacteria. II. Screening of antibiotic producers and taxonomical properties of Gluconobacter sp. W-315.
Antibiotic producing bacteria were selected using a new screening method. Eight strains of antibiotic producing bacteria, which required a spent medium of fungi for antibiotic production, were isolated. One of them, a potent producer of antibacterial antibiotic, designated strain W-315, had following taxonomical characteristics; aerobic, Gram-negative, rod shaped and polar flagellated. Furthermore, the organism could grow under acidic conditions (pH 4.5) and had a GC content of 64.4 mole per cent. We concluded that the strain W-315 belonged to Gluconobacter sp. When this bacterium was inoculated into Czapek-Dox medium, bacterial growth and antibiotic production did not occur. The antibiotic production was also not observed even when poor growth was observed in Czapek-Dox medium supplemented with ammonium sulfate. The nutritional requirements for the antibiotic production were also discussed.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents; D001230:Aspergillus / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D002606:Charcoal; D004269:DNA, Bacterial / Q000032:analysis; D004353:Drug Evaluation, Preclinical; D009492:Neurospora crassa / Q000378:metabolism; D011548:Pseudomonadaceae / Q000145:classification / Q000378:metabolism*; D012233:Rhizopus / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004269:DNA, Bacterial; D002606:Charcoal