title: Control of phage lambda development by stability and synthesis of cII protein: role of the viral cIII and host hflA, himA and himD genes.
abstract: The cII protein of bacteriophage lambda has a decisive role in the regulatory switch between the lysogenic and lytic pathways of viral development. Recent work has indicated that cII may be the primary control function providing for the initial partition between the two pathways, with other host and viral regulatory genes acting to determine the levels of cII in an infected cell. We have studied the synthesis and stability of cII protein with two experimental systems, phage infection and a cII-producing plasmid. We have found that the stability of cII is controlled by the host hflA and viral cIII genes; hflA protein facilitates degradation of cII, whereas cIII protects cII. The synthesis of cII appears to be under the positive control of the host himA and himD genes. We conclude that posttranscriptional regulation of cII by host and viral genes is critical for the choice of a developmental pathway.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D004355:Drug Stability; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005809:Genes, Regulator; D005814:Genes, Viral; D007700:Kinetics; D008242:Lysogeny; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins