The Vibratome-Ralph knife combination: a useful tool for immunohistochemistry.
A modified immunohistochemical technique is described for the improved detection of antigens. The method involves the use of the Vibratome combined with Ralph knives, which are easily manufactured with an LKB 2078 Histoknifemaker. The sectioning procedure was used with the peroxidase-antiperoxidase method of Sternberger and his collaborators for demonstrating growth hormone release-inhibiting hormone (somatostatin) and noradrenaline N-methyltransferase in neurones in the central nervous system of the rat. The morphological preservation of the tissue was good and cytological details were easily seen, especially in the thin sections (5-10 microns thick).
D000818:Animals; D001921:Brain / Q000166:cytology / Q000276:immunology; D006651:Histocytochemistry / Q000295:instrumentation* / Q000379:methods; D007120:Immunochemistry / Q000295:instrumentation* / Q000379:methods; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008297:Male; D009474:Neurons / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013047:Specific Pathogen-Free Organisms