ADP-ribosylation of isolated rat islets of Langerhans.
Incubation of isolated rat islets of Langerhans with [adenine-2,8-3H]NAD+ results in rapid incorporation of 3H into acid-insoluble products. The major site of incorporation appears to be the cell membrane. The reaction is inhibited by nicotinamide, an ADP-ribosylation inhibitor, and stimulated by arginine, an ADP-ribose acceptor. The results demonstrate that islet membrane proteins can be ADP-ribosylated in the absence of exogenous ADP-ribosylating agents and suggest that ADP-ribosylation plays a role in pancreatic islet cell function.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009536:Niacinamide / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D013806:Theophylline / Q000494:pharmacology; D014316:Tritium
D009702:Nucleoside Diphosphate Sugars; D009243:NAD; D014316:Tritium; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; D001120:Arginine; D013806:Theophylline