Use of imprints for monoclonal antibody studies: suitability of air-dried preparations from lymphoid tissues with an immunohistochemical method.
Air-dried imprint preparations are conveniently produced from human lymphoid samples without the special methods required for snap-freezing tissues or rendering them into suspensions. T-cells, T-cell subsets (helper and suppressor), and HLA-DR-positive cells (B-lymphocytes, monocytic-histiocytic cells) can be identified in such imprints by the use of commercially obtained mouse hybridoma antibodies with a simple two-step immunoperoxidase method. Direct nuclear morphologic correlation with surface determinants is achieved by this method. Immunoreactivity is retained only eight to 10 days in such air-dried preparations, and attempts to prolong reactivity have been unsuccessful so far.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000032:analysis
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II