Immunogenetic analysis of the HLA complex with alloreactive T cell clones.
Recently developed technologies to clone alloreactive human lymphocytes have provided a powerful tool for the immunogenetic analysis of the HLA region. Alloreactive T cell clones can be used as specific reagents for HLA encoded cellular determinants detected in secondary proliferation assays such as the primed lymphocyte test (PLT) and, in cell-mediated lympholysis (CML). Lymphocyte clones can also be used for functional assays (e.g., for suppressor or helper activity) to determine the influence of HLA specific restriction.
D002999:Clone Cells; D005802:Genes, MHC Class II; D006680:HLA Antigens / Q000145:classification / Q000235:genetics*; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000145:classification; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology*
D006680:HLA Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II