Human B-like lymphoblastoid cell lines obtained by long-term culture of normal spleen leukocytes.
B-like lymphoblastoid cell lines were obtained by long-term culture of human spleen leukocytes in RPMI 1640 medium containing human plasma fraction instead of whole foetal calf serum. These cell lines, which did not form E-rosettes had membrane immunoglobulins, and expressed Epstein-Barr virus antigens. Most synthesized intracytoplasmic immunoglobulins and were shown to be diploid and to remain so after subcultures. All produced interferon upon induction with Sendai virus.
D001402:B-Lymphocytes / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D013154:Spleen / Q000502:physiology