A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa.
A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa has been developed. The procedure is sensitive enough to detect Escherichia coli plasmid pBR322 DNA integrated into chromosomal DNA in a Neurospora transformant. Thus, it should facilitate the isolation of nuclear genes by plasmid-rescue procedures.
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004271:DNA, Fungal / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D005374:Filtration; D006824:Hybridization, Genetic; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids
D004269:DNA, Bacterial; D004271:DNA, Fungal