Inhibitory effect of deoxynivalenol, 3-acetyldeoxynivalenol and zearalenone on induction of rat and human lymphocyte proliferation.
Deoxynivalenol (DON), 3-acetyldeoxynivalenol (acetylDON) and zearalenone (Ze) were examined for their in vitro effect on mitogen-induced lymphocyte blastogenesis using rat or human peripheral blood lymphocytes (PBL). A dose-dependent reduction of lymphocyte proliferation was demonstrated for each mycotoxin. However, the inhibitory effect of DON was significantly higher than that of the acetylated compound. Concentrations of 90 ng/ml and 220 ng/ml inhibited rat and human lymphocyte blastogenesis by 50%, respectively, whereas 450 ng/ml and 1060 ng/ml acetylDON were required to produce the same effect. The amount of Ze necessary to inhibit blastogenesis by 50% was 250 times greater than required for DON. There was no evidence of cell death and combinations of DON and Ze did not alter the expected response.
D000818:Animals; D004357:Drug Synergism; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D012118:Resorcinols / Q000633:toxicity*; D012717:Sesquiterpenes / Q000633:toxicity*; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*; D015025:Zearalenone / Q000633:toxicity*
D010835:Phytohemagglutinins; D012118:Resorcinols; D012717:Sesquiterpenes; D014255:Trichothecenes; C043247:3-acetyldeoxynivalenol; D015025:Zearalenone; C007262:deoxynivalenol; D013936:Thymidine