Dopamine and 5-HT uptake by synaptosomes after pretreatment with p-chlorophenylalanine.
5-Hydroxytryptamine (5-HT) and dopamine uptake were studied in rat hypothalamic and striatal synaptosomes, respectively, after a single-dose or repeated doses of p-chlorophenylalanine (PCPA), and after withdrawal of the drug. 5-HT uptake did not change 24 h after a single large dose of PCPA (300 mg/kg). Uptake of neither amine changed 12 h or 7 days after treatment with 300 mg/kg PCPA every third day, for 2 weeks. In all cases, the kinetic parameters remained identical. These results indicate that depletion of 5-HT levels by PCPA and the resulting decrease in serotoninergic activity do not induce changes in 5-HT or dopamine uptake. Hence, reported changes in transmitter uptake due to clinical or experimental manipulations must not be directly related to synaptic activity.
D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D010134:Fenclonine / Q000494:pharmacology*; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D012701:Serotonin; D010134:Fenclonine; D004298:Dopamine