title: Sulfite suppresses the mutagenic property of coffee.
abstract: The mutagenicity of instant and freshly brewed coffee on Salmonella typhimurium TA100 and TA98 without S9 mix was inactivated by sodium sulfite. Sulfite ion at a dose of 200 ppm almost completely inactivated the mutagenicity of coffee made in the ordinary way (5-15 mg dry weight/ml). Sodium bisulfite and potassium metabisulfite had similar effects. On the contrary, L-ascorbic acid enhanced the mutagenicity of coffee. Sodium sulfite also inactivated the phage-inducing activity of coffee in inductest III. Sodium sulfite completely suppressed the mutagenicities of 1,2-dicarbonyls, namely diacetyl and glyoxal. Diacetyl is present in coffee, beer, butter and other foods and drinks. Because sodium sulfite, sodium bisulfite and potassium metabisulfite are widely used as food additives, they should be useful in reducing the levels of mutagens in foods.
mesh_terms: D010582:Bacteriophage lambda / Q000187:drug effects; D003069:Coffee / Q000032:analysis*; D004926:Escherichia coli / Q000187:drug effects; D006358:Hot Temperature; D009152:Mutagenicity Tests; D009153:Mutagens / Q000302:isolation & purification / Q000494:pharmacology*; D009154:Mutation; D013447:Sulfites / Q000494:pharmacology*
chemical_list: D003069:Coffee; D009153:Mutagens; D013447:Sulfites; C025026:sodium sulfite