The ratio between intrinsic 115 kDa and 30 kDa peptides as a marker of fibre type-specific sarcoplasmic reticulum in mammalian muscles.
Sarcoplasmic reticulum displays characteristic differences in Ca2+-uptake, Ca2+-ATPase and the pattern of membrane proteins in type I, IIA and IIB fibres. The ratio between the 115 kDa Ca2+-ATPase and a 30 kDa protein is of characteristic magnitude in the sarcoplasmic reticulum of the three fibre types in rat muscles. The slow-to-fast fibre type transformation observed in rabbits during chronic nerve stimulation is accompanied by predictable changes of this ratio.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D010455:Peptides; D000252:Calcium-Transporting ATPases; D002118:Calcium