Histochemical and biochemical characteristics of the transient hypertrophy of the denervated rat hemidiaphragm.
A systematic study of the different fiber types of rat diaphragm muscle in the first 10 days after unilateral denervation showed approximately a 10% decrease in diameter of the white fibers, 25% increase in that of intermediate fibers, and 35% increase in that of red fibers together with diminished differential staining properties both for myosin ATPase and Sudan black. There was also a doubling of the amount of connective tissue. A reduction in total lipid concentration of the tissue included decreases in both triacylglycerol and phospholipid, but not cholesterol. Magnesium concentration in the tissue also declined, as did activity of Ca2+-activated, though not Mg2+-activated, myosin ATPase.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003964:Diaphragm / Q000294:innervation / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006984:Hypertrophy; D050356:Lipid Metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D009121:Muscle Denervation; D051381:Rats; D011919:Rats, Inbred Strains
D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium