Determination of the oligomeric structure of the Ca2+ pump protein in canine cardiac sarcoplasmic reticulum membranes using radiation inactivation analysis.
The size of the Ca2+ pump protein in canine cardiac sarcoplasmic reticulum (SR) membranes was determined using target theory analysis of radiation-inactivation data. Samples of cardiac SR were irradiated in the frozen state with increasing doses of high energy electrons from a Van de Graaff accelerator. The loss of Ca2+-dependent ATPase activity and of ATP-dependent, oxalate-facilitated Ca2+ uptake (loading) with increasing irradiation dosage paralleled one another. Also, the loss of staining intensity of the predominant polypeptide (Mr = 110,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis correlated with the loss of ATPase and loading activities. The target size by all three measurements varied between 213,000 and 229,000 Da. We conclude that the Ca2+ pump protein in canine cardiac SR is a dimer.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects*; D004285:Dogs; D004307:Dose-Response Relationship, Radiation; D046911:Macromolecular Substances; D008722:Methods; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology* / Q000528:radiation effects
D046911:Macromolecular Substances; D000252:Calcium-Transporting ATPases