title: Neocarzinostatin abstracts a hydrogen during formation of nucleotide 5'-aldehyde on DNA.
abstract: The oxidative reaction of polydeoxyadenylic-deoxythymidylic acid [poly(dA-dT)] with neocarzinostatin that produces 5'-thymidine aldehyde esterified to the 5'-end of strand breaks proceeds with hydrogen abstraction. The abstracted hydrogen is covalently bound to the non-protein component of neocarzinostatin; only a small amount (5%) is washed out into solvent. These data rule out a peroxyl radical as the primary DNA damaging species involved in the production of the 5'-aldehyde group. In contrast to earlier reports, it is demonstrated that alpha-tocopherol is not an inhibitor of the reaction.
mesh_terms: D000447:Aldehydes; D000903:Antibiotics, Antineoplastic; D055598:Chemical Phenomena; D002621:Chemistry; D007700:Kinetics; D011067:Poly dA-dT / Q000138:chemical synthesis; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D013936:Thymidine; D014316:Tritium; D009353:Zinostatin
chemical_list: D000447:Aldehydes; D000903:Antibiotics, Antineoplastic; D011089:Polydeoxyribonucleotides; D014316:Tritium; D011067:Poly dA-dT; D009353:Zinostatin; D013936:Thymidine