Dissociation and isolation of chromatin proteins in salt solutions by an aqueous two-phase system.
An aqueous two-phase system containing 7% Dextran T 500-5% polyethylene glycol (PEG) 6000 has been adopted for rapid selective stepwise extractions of high-mobility-group proteins and histones from both isolated chromatin and intact nuclei of calf thymus. After the dissociated proteins in the PEG phase were precipitated with 20% trichloroacetic acid at 4 degrees C, proteins were recovered from this phase by solubilization of PEG with acidified acetone at room temperature. This method allows preparation of nuclei depleted of histone H1.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000302:isolation & purification*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins; D006657:Histones / Q000302:isolation & purification; D009698:Nucleoproteins / Q000302:isolation & purification*; D013950:Thymus Gland / Q000032:analysis
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D006657:Histones; D009698:Nucleoproteins