Inhibition of human B lymphocyte differentiation by a stable metabolite of cyclophosphamide (ASTA Z 7557, INN mafosfamide).
We have evaluated the immunosuppressive effect of a new stable derivative of cyclophosphamide (ASTA Z 7557) on human lymphocyte immunoglobulin biosynthesis in vitro. When graded amounts of the drug are added to lymphocyte cultures stimulated with the helper T cell-dependent activator (PWM), a marked inhibition of B cell proliferation and differentiation occurs. Lymph node cells are particularly sensitive to the drug, while splenocytes are relatively resistant. The agent exerts only a minor effect on immunoglobulin synthesis triggered by a direct B cell stimulant (S. paratyphi bacteria).
D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D007136:Immunoglobulins; D011043:Pokeweed Mitogens; C037933:Asta Z 7557; D003520:Cyclophosphamide