Effect of medroxyprogesterone acetate upon PHA, Con A and PWM stimulated lymphocytes and on E-rosette function.
We evaluated if Medroxyprogesterone acetate (MPA) had an immunosuppressive action as progesterone and other steroid hormones. Lymphocyte function was evaluated by the blastogenic response to mitogens and by the E-rosette test. MPA was tested at 7 ng/ml and 100 ng/ml concentrations. Cells were either preincubated for 30 min., 90 min., 180 min. and 12 hr or incubated with MPA for 72 hr, 18 hr and 16 hr. MPA showed a significative inhibitory effect only when used at the concentration of 100 ng/ml.
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008525:Medroxyprogesterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D017258:Medroxyprogesterone Acetate; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012397:Rosette Formation
D007166:Immunosuppressive Agents; D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D017258:Medroxyprogesterone Acetate; D008525:Medroxyprogesterone