Echinocandin inhibition of 1,3-beta-D-glucan synthase from Candida albicans.
The cyclic peptide antibiotic echinocandin was found to inhibit 1,3-beta-D-glucan synthase activity present in a mixed membrane fraction from Candida albicans. Addition of antibiotic reduced the Vmax of the enzyme, but the Km was unaffected. GTP stimulated enzyme activity approximately 4-fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with alpha-amylase and beta-glucanase confirmed that the polymer synthesised was 1,3-beta-D-glucan, not glycogen.
D000900:Anti-Bacterial Agents; D002176:Candida albicans / Q000201:enzymology*; D054714:Echinocandins; D005656:Fungal Proteins; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007700:Kinetics; D008565:Membrane Proteins; D010455:Peptides / Q000494:pharmacology; D010456:Peptides, Cyclic; D029702:Schizosaccharomyces pombe Proteins
D000900:Anti-Bacterial Agents; D054714:Echinocandins; D005656:Fungal Proteins; D008565:Membrane Proteins; D010455:Peptides; D010456:Peptides, Cyclic; D029702:Schizosaccharomyces pombe Proteins; D006160:Guanosine Triphosphate; C007572:echinocandin B; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase