[Isolation of nonlysogenic bacteria from lysogenic ones using a phage carrying resistance to antibiotics].
The method for obtaining nonlysogenic bacteria from lysogenic ones by means of a phage carrying resistance to antibiotics is proposed. Solitary nonlysogenic cells in a lysogenic culture are lysogenized after the infection of the culture with a labeled phage and then harvested on a selective medium: under special conditions the phage is eliminated from the cells.
D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors*; D001431:Bacteriological Techniques; D010582:Bacteriophage lambda / Q000187:drug effects / Q000302:isolation & purification*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification*; D008242:Lysogeny; D014775:Virus Activation
D000900:Anti-Bacterial Agents
Poluchenie nelizogennykh bakteriĭ iz lizogennykh pri pomoshchi faga, nesushchego ustoĭchivost' k antibiotikam.