Effects of vanadate on intracellular Ca2+ redistribution and hexose transport across plasma membrane in cultured mouse fibroblasts.
The effects of vanadate on intracellular Ca2+ sequestration and hexose transport were studied in Swiss 3T3 cells. Vanadate inhibited ATP-dependent Ca2+ uptake by saponin-permeabilized Swiss 3T3 cells at 10(-5) and 10(-7) M Ca2+ at which the Ca2+ uptake was sensitive and insensitive to oligomycin plus antimycin A, respectively. On the other hand, vanadate stimulated 2-deoxy-D-glucose (2DG) uptake in a dose- and time-dependent way. The stimulation of 2DG uptake by vanadate was inhibited by EGTA plus A23187 and the inhibition was reversed by Ca2+ restoration. These results suggest that an increase in cytosolic Ca2+ by inhibition of intracellular ATP-dependent Ca2+ sequestration by vanadate results in the stimulation of hexose transport in Swiss 3T3 cells.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009004:Monosaccharide Transport Proteins; D009840:Oligomycins / Q000494:pharmacology; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D002352:Carrier Proteins; D003837:Deoxy Sugars; D009004:Monosaccharide Transport Proteins; D009840:Oligomycins; D014639:Vanadium; D014638:Vanadates; D004533:Egtazic Acid; D004815:Epidermal Growth Factor; D000968:Antimycin A; D003847:Deoxyglucose; D000252:Calcium-Transporting ATPases; D002118:Calcium