Improved coating and fixation methods for scanning electron microscope autoradiography.
A simple apparatus for emulsion coating is described. The apparatus is inexpensive and easily assembled in a standard glass shop. Emulsion coating for scanning electron microscope autoradiography with this apparatus consistently yields uniform layers. When used in conjunction with newly described fixation methods, this new approach produces reliable autoradiographs of undamaged specimens.
D001120:Arginine / Q000378:metabolism; D001345:Autoradiography / Q000379:methods*; D002696:Chlamydomonas / Q000648:ultrastructure; D004655:Emulsions; D005976:Glutaral; D047348:Hydrolyzable Tannins; D007202:Indicators and Reagents; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D009993:Osmium Tetroxide; D014316:Tritium
D004655:Emulsions; D047348:Hydrolyzable Tannins; D007202:Indicators and Reagents; D014316:Tritium; D001120:Arginine; D009993:Osmium Tetroxide; D005976:Glutaral