Effects of indomethacin on lymphocyte proliferation, suppressor cell function, and leukocyte migration inhibitory factor (lmif) production.
The in vitro effect of indomethacin (IND) on PHA- and Con-A-induced immune responses was studied. The results showed that the enhancement of both PHA and Con A responses by IND does not significantly depend on the concentrations of mitogens or IND. A significant negative correlation was found between the initial PHA response and augmentation index, whereas there was a positive correlation between the initial Con A response and the enhancement of the Con A responses. Indomethacin significantly increased the Con-A-induced suppressor cell activity and PHA-induced leukocyte migration inhibitory factor production (LMIF), whereas the effects on the PHA-induced suppressor cell activity and Con-A-induced LMIF production were not significant. The findings indicate the indomethacin has a dual effect on PHA- and Con-A-induced immune responses probably reflecting intrinsic differences among T cells, for example, distinct lymphokines produced by different T-cell subpopulations.
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008222:Lymphokines / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010835:Phytohemagglutinins; D003208:Concanavalin A; D007213:Indomethacin