[Indices of partial oxygen deprivation in the culture medium of cardiac cells].
Three indexes of partial oxygen deprivation, i.e. hypoxanthine, alpha HBDH and CK, were investigated in rat heart cell cultures, 7 day-old. Enzyme release in the medium and hypoxanthine uptake by the cells pointed out both oxygen and glucose deprivation, which modelized ischemia. Conversely, hypoxanthine release pointed out oxygen deprivation, in the presence of glucose however, which modelized hypoxia, whereas there was no enzyme leakage in the latter condition.
D000693:Anaerobiosis; D000818:Animals; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism*; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism*; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007042:Hypoxanthines; D019271:Hypoxanthine; C035749:2-hydroxybutyrate dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D003402:Creatine Kinase
Etude de quelques indices de la privation partielle d'oxygène dans le milieu de culture de cellules cardiaques.