Fractionation and isolation of heparan sulfates using poly-L-lysine-Sepharose.
A simple procedure for the isolation of heparan sulfates from pig lung using a poly-L-lysine-Sepharose column is described. Glycosaminoglycans are absorbed on poly-L-lysine-Sepharose at pH 7.5 and eluted with an NaCl linear gradient in the following order: hyaluronic acid (0.32 M NaCl), chondroitin (0.36 M NaCl), keratan sulfate (0.80 M NaCl), chondroitin 4-sulfate (0.86 M NaCl), chondroitin 6-sulfate (0.95 M NaCl), dermatan sulfate (0.91 M NaCl), heparan sulfate (1.2 M NaCl), and heparin (1.35 M NaCl). Based on these observations, isolation of heparan sulfate from pig lung crude heparan sulfate fractions which contain chondroitin sulfates and dermatan sulfate was attempted, using this chromatographic technique.
D000818:Animals; D002807:Chondroitin / Q000302:isolation & purification; D002847:Chromatography, Agarose / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D006493:Heparin / Q000302:isolation & purification; D006497:Heparan Sulfate / Q000302:isolation & purification*; D006820:Hyaluronic Acid / Q000302:isolation & purification; D007632:Keratan Sulfate / Q000302:isolation & purification; D008168:Lung / Q000032:analysis; D012685:Sepharose / Q000031:analogs & derivatives*; D013053:Spectrophotometry; D013431:Sulfates / Q000302:isolation & purification*; D013552:Swine
D002807:Chondroitin; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D007632:Keratan Sulfate; D012685:Sepharose; D013431:Sulfates; C030675:lysine-sepharose