In vitro effects of 4-hydroperoxycyclophosphamide on concanavalin A-induced human suppressor T cells.
Treatment in vitro of human peripheral blood lymphocytes (PBL) with ConA induced the generation of suppressor cells which inhibited T cell blastogenic response to ConA and of allogeneic response in the mixed lymphocyte reaction (MLR). Treatment of PBL with 4-hydroperoxycyclophosphamide (4-HPCy) before incubation with ConA markedly decreased the generation of suppressor cells by ConA. The effect of 4-HPCy on generation of suppressor cells was more pronounced in the test of ConA stimulation than in the MLR. Treatment with 4-HPCy had no effect on suppressor cells already induced as shown by incubation of PBL with 4-HPCy after incubation with ConA.
D002478:Cells, Cultured; D003208:Concanavalin A; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
D003208:Concanavalin A; D003520:Cyclophosphamide; C011272:perfosfamide