Purification of human platelet membrane glycoproteins IIb and IIIa using high-performance liquid chromatography gel filtration.
A method for purifying platelet membrane glycoproteins IIb and IIIa to homogeneity has been developed. The procedure involves high-pressure gel filtration chromatography using a TSK-4000SW column in the presence of sodium dodecyl sulfate. This technique is capable of rapidly preparing milligram quantities of each glycoprotein with greater than 90% recovery. The use of this technique should aid in defining the structural and functional properties of GPIIb and GPIIIa.
D001792:Blood Platelets / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification*; D010980:Platelet Membrane Glycoproteins; D011092:Polyethylene Glycols
D006023:Glycoproteins; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D011092:Polyethylene Glycols