Radioimmunoassay of atrial natriuretic factor (ANF) in rat atria.
We describe a solid phase radioimmunoassay for atrial natriuretic factor (ANF) and its application for measurement of this peptide in homogenates of rat atria. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Sample (or standard) are incubated with the rabbit anti-8-33 ANF antiserum in peptide (8-33 ANF)-coated wells. Then an excess of I125 goat anti-rabbit IgG is added. The radioactivity bound is directly proportional to the amount of ANF present. The concentration of immunoreactive ANF has been found to be about 4 times higher in the right atrium than in the left atrium of the rat.
D000818:Animals; D009320:Atrial Natriuretic Factor; D006325:Heart Atria / Q000032:analysis*; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D009318:Natriuresis; D011863:Radioimmunoassay; D051381:Rats
D009124:Muscle Proteins; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor