A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases.
A DNA sequence consisting of the 5-mer AGAGG repeated tandemly 32 times has been detected in a chicken genomic clone and found to be present in about 2000 copies per chicken genome. This sequence was highly susceptible to single-strand specific endonucleases isolated from Aspergillus oryzae (S1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from Neurospora crassa occurred only at a pH below 5.5. Endonucleolytic cutting of the AGAGG sequence by the single-strand specific enzymes required a supercoiled substrate and was independent of ionic strength.
D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA; D004277:DNA, Single-Stranded; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000201:enzymology; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D004247:DNA; D004706:Endodeoxyribonucleases