Isolation of an enzyme system in rat lung cytosol which converts arachidonic acid into 8,11,12-trihydroxyeicosatrienoic acid.
An enzyme system in rat lung is described which transforms arachidonic acid into 8,11,12-trihydroxyeicosatrienoic acid. The enzyme system is present in the high-speed supernatant fraction of a rat lung homogenate and can be precipitated with ammonium sulfate (30 to 50% saturation) and concentrated. Preliminary experiments indicate that the enzyme activity can be eluted from Sephadex G-200 and Sepharose 6B gel chromatography, whereas all activity is lost on DEAE cellulose chromatography. Enzyme activity is heat-liable, being lost after 10 min at 50 degrees C.
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D007536:Isomerism; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; C019121:8,11,12-trihydroxy-5,9,14-eicosatrienoic acid; D015126:8,11,14-Eicosatrienoic Acid