[Effect of MEA on DNA degradation in permeable irradiated Bac. stearothermophilus cells].
It was shown that DNA-degrading activity of permeable, intact and gamma-irradiated cells of Bac. stearothermophilus decreased under the effect of beta-mercaptoethylamine (MEA). MEA decreased also a DNAase activity, in a crude acellular extract of Bac. stearothermophilus, and activities of S1-nuclease and DNAase I. The data obtained prompt an assumption that MEA has an inhibitory action on the activity of endonucleases irrespective of their substrate specificity.
D002463:Cell Membrane Permeability; D004269:DNA, Bacterial / Q000378:metabolism* / Q000528:radiation effects; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D001411:Geobacillus stearothermophilus / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008624:Mercaptoethylamines / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation / Q000187:drug effects* / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D004269:DNA, Bacterial; D008624:Mercaptoethylamines; D011837:Radiation-Protective Agents; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I
Vliianie MEA na degradatsiiu DNK v pronitsaemykh obluchennykh kletkakh Bac. stearothermophilus.