Demonstration of Fc and C3b receptors on rat perikarya.
Minced rat brain deprived of cerebellum was dissociated by trituration through stainless steel screen and nylon meshes, then by velocity sedimentation technique free-floating perikarya were separated from cell syncytia and cellular debris. The presence of Fc-receptor and C3b-receptor activities, as well as the absence of membrane-bound immunoglobulin and receptor for sheep blood cells were demonstrated on separated perikarya of the rat brain.
D000818:Animals; D001921:Brain / Q000166:cytology; D002458:Cell Fractionation; D066298:In Vitro Techniques; D009474:Neurons / Q000032:analysis*; D051381:Rats / Q000502:physiology*; D011919:Rats, Inbred Strains; D011951:Receptors, Complement / Q000032:analysis*; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000032:analysis*; D012397:Rosette Formation
D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc