title: Feline one-way mixed leukocyte reaction.
abstract: The feline one-way mixed leukocyte reaction (MLR) was accomplished by a microtechnique assay using gradient purified mononuclear leukocytes from the peripheral blood of specific-pathogen-free (SPF) cats. An eight day assay was required with an appropriate serum supplement of rabbit or cat serum and obtainable cell concentrations of 1 X 10(5) mononuclear cells per well of each responder and stimulator population. This assay was devised as an in vitro correlate of cell-mediated immunity and to demonstrate that feline histocompatibility differences can be detected in culture.
mesh_terms: D000818:Animals; D002415:Cats / Q000235:genetics / Q000276:immunology*; D006648:Histocompatibility; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*
chemical_list: D006649:Histocompatibility Antigens