[Specificity of cell-mediated cytotoxicity. II. Cytotoxic lymphocytes after in vitro allogeneic sensitization in the H-2 system].
Allogeneic intraperitoneal immunization in vivo leads to induction of cytotoxic lymphocytes. The cytotoxic reaction of these lymphocytes is specific in vitro against thymocytes as target cells and depends on identical private determinants of stimulator and target cell haplotype. Cross reactions are not observed in this model with H-2 incompatible haplotypes as target cells, also no autoreactivity is found. But if effector cells are used after stimulation in a mixed lymphocyte culture and mitogen stimulated spleen cells as target cells, cross reaction are generally found and autoreactivity too. Conditions of the trial determine the results. In vivo and in vitro induced effector cells destroy thymocytes as well as spleen cell blasts. The type of target cell does not influence the specificity of the cytotoxic reaction. Only in vivo induced effector cells react specifically.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology*; D007106:Immune Sera; D007114:Immunization; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D006183:H-2 Antigens; D007106:Immune Sera; D003208:Concanavalin A
Zur Spezifität der zellvermittelten Zytotoxizität. II. Zytotoxische Lymphozyten nach allogener Sensibilisierung in vitro im H-2-System.