Function and structure of microvirid phage alpha 3 genome. II. Isolation and properties of various mutants of alpha 3.
Various mutants were isolated from a microvirid (isometric single-stranded DNA) phage alpha 3, by mutagenesis with hydroxylamine or nitrous acid. They were divided into eight complementation groups, and mainly by genetic crosses the gene alignment was determined as -A-B-C'-D-J'-F-G-H-. Except for groups C' and J', each defective gene product was clearly discerned in electropherograms of proteins extracted from the phage-infected suppressor-negative (Su-) Escherichia coli. Only gene A mutants abolished synthesis of the progeny replicative-form DNA (RF), whereas mutants belonging to groups B, C', D, E, F and J' affected RF replication at late stage, as well as synthesis of the single-stranded DNA (SS). Additional properties of several mutants are also discussed.
D010584:Bacteriophage phi X 174 / Q000235:genetics; D002118:Calcium / Q000494:pharmacology; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005816:Genetic Complementation Test; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D009154:Mutation; D009608:Nitrous Acid / Q000494:pharmacology; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006898:Hydroxylamines; D014764:Viral Proteins; D019811:Hydroxylamine; D002118:Calcium; D009608:Nitrous Acid