Epitopes immunologically related to glycophorin A on human malignant and non-malignant cells in culture.
Nine monoclonal antibodies specific to different antigenic determinants on the cyanogen bromide fragments CNBr3 (1-8) and CNBr1 (9-81) of glycophorin A, the major sialoglycoprotein of human red blood cells, were used for the detection of similar or cross-reactive epitope(s) on nucleated somatic cells. A panel of human malignant and non-malignant cells consisting of erythroleukemia, melanoma and carcinomas of breast, cervix, larynx, nasopharynx and colon, as well as human fibroblasts and mammary cells, were tested using a sensitive enzyme-linked immunoassay. The presence of glycophorin-like epitopes were demonstrated, in varying concentrations, on all cells tested. These epitropes were found to be localized on the cell membranes by indirect immunofluorescence.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006021:Glycophorins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009369:Neoplasms / Q000276:immunology*; D011863:Radioimmunoassay; D012795:Sialoglycoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006021:Glycophorins; D012795:Sialoglycoproteins