A rapid method for preparing avian tracheal rings for organ culture.
A simple, rapid method for preparing rings from chicken tracheas is described. Using a polyacrylamide disc gel slicer, up to 100 uniform tracheal rings were obtained from two tracheas with a single cut and without injury to the ciliated tracheal epithelium. The responses of rings cut with a disc gel slicer and those cut conventionally with a scalpel blade were compared by virus titration, virus neutralization, and viability of uninfected 18-day-old-chick embryo rings over a nine-day incubation period. Rings cut with a disc gel slicer did not differ significantly from those cut conventionally with respect to virus titer, neutralization indices, or viability. A disc gel slicer expedites the preparation of functional rings and offers an alternative to the present method of cutting tracheal rings with a scalpel blade.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D009924:Organ Culture Techniques / Q000379:methods*; D013047:Specific Pathogen-Free Organisms; D014132:Trachea; D014776:Virus Cultivation