title: Induction of granulocyte histaminase release by particle-bound complement C3 cleavage products (C3b, C3bi) and IgG.
abstract: The interaction of opsonized particles with human granulocytes promotes a number of important biologic functions, including phagocytosis, superoxide generation, and release of a variety of enzymes, including histaminase. We have previously determined that histaminase release occurs via a C3-dependent process. Although fluid-phase C3b dimers can mediate release, the relative effects of particle-bound C3b and C3bi and of IgG have not been examined. In this report we demonstrate that particle-bound C3 deposited on activators of the alternative C pathway effected histaminase release in the absence of IgG. Particle-bound C3bi and C3b were both effective as mediators of histaminase release. The extent of release varied as a function of the activating surface on which C3 was deposited (zymosan C3b was considerably more potent than C3b bound to rabbit erythrocytes, which was slightly more potent than C3b bound to neuraminidase-treated sheep erythrocytes). In contrast, C3b or C3bi deposited on nonactivating surfaces (such as sheep erythrocytes) at inputs of up to 2,000,000 molecules per granulocyte failed to induce histaminase release unless IgG was also present. The ability of C3b bound to particles that serve as activators of the alternative pathway to induce histaminase release is apparently not the result of decreased susceptibility of C3b to proteolysis or to an increased binding affinity to the C3b receptor, but may relate to the interaction of other surface structures on activating particles with the PMN membrane.
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D003176:Complement C3 / Q000276:immunology; D003179:Complement C3b / Q000032:analysis / Q000502:physiology*; D003180:Complement C3b Inactivator Proteins / Q000097:blood / Q000502:physiology*; D015932:Complement C3c; D003170:Complement Pathway, Alternative; D004910:Erythrocyte Membrane / Q000378:metabolism; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D051379:Mice; D010447:Peptide Hydrolases / Q000097:blood; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D007074:Immunoglobulin G; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D003179:Complement C3b; D015932:Complement C3c; D015054:Zymosan; D006631:Amine Oxidase (Copper-Containing); D010447:Peptide Hydrolases