Reconstitution of stellacyanin as a case of direct Cu(I) transfer between yeast copper thionein and 'blue' copper apoprotein.
It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu-thionein, without any detectable unspecific Cu(II) intermediate, both aerobically and anaerobically.
D001059:Apoproteins / Q000378:metabolism*; D002414:Cations, Monovalent; D003300:Copper / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism; D009492:Neurospora crassa / Q000378:metabolism; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D001059:Apoproteins; D002414:Cations, Monovalent; D005656:Fungal Proteins; D008667:Metalloproteins; D010940:Plant Proteins; C003343:stellacyanin protein, plant; D003300:Copper; D008668:Metallothionein