Expression of the marker (X) (q28) in lymphoblastoid cell lines.
The marker(X)(q28) chromosome associated with one type of X-linked mental retardation has been demonstrated in lymphoblastoid cell lines established from affected individuals. The mar(x) can reliably and repeatedly be seen by the addition of FUdR to the cultures for 24 hrs prior to harvest. This simple technique provides an excellent in vitro experimental test system for investigation of the mar(X).
D002460:Cell Line; D003470:Culture Media; D005260:Female; D005467:Floxuridine; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D066298:In Vitro Techniques; D008607:Intellectual Disability / Q000235:genetics*; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D012730:Sex Chromosomes; D014960:X Chromosome
D003470:Culture Media; D005819:Genetic Markers; D005467:Floxuridine