[Metabolism of dihydrotestosterone in cultured skin fibroblasts: reduction to 5 alpha-androstane-3 alpha, 17 beta-diol].
Reduction of dihydrotestosterone into 5 alpha-androstan-3 alpha, 17 beta diol by 3 alpha hydroxysteroid dehydrogenase was studied in human cultured skin fibroblasts. Characterization of the 3 alpha diol was performed by gas-liquid chromatography coupled with mass spectrometry. Sex skin fibroblasts enzyme has the same Km (9.10 X 10(-6) M) as that of non sex skin fibroblasts (8.75 X 10(-6) M). On the other hand, reduction of DHT is 3 times higher in sex skin fibroblasts (Vmax = 223.9) than in non sex skin fibroblasts (Vmax = 87.9). Thus, human culturel fibroblasts appear again to be an useful tool for the study of a key-enzyme of androgen metabolism in target cells.
D015113:Androstane-3,17-diol / Q000378:metabolism*; D000732:Androstanols / Q000378:metabolism*; D002478:Cells, Cultured; D002849:Chromatography, Gas; D013196:Dihydrotestosterone / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005837:Genitalia, Male; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D012867:Skin / Q000166:cytology
D000732:Androstanols; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol
Métabolisme de la dihydrotestostérone dans le fibroblaste cutané en culture: réduction en 5 alpha-androstane 3 alpha, 17 beta diol.