Cortisone-induced small RNP tightly bound to chromatin.
The small nuclear RNP (alpha-RNP) tightly bound to chromatin has been isolated. alpha-RNP can be removed from chromatin together with the acid-soluble proteins. The RNA from this RNP has been isolated; its electrophoretic mobility is equal to that of 4 S RNA. The study of the resistance of alpha-RNA to RNases (A, T1 and S1) in salt solutions of various ionic strengths allows us to conclude that the alpha-RNA has a well-developed secondary structure. The alpha-RNA is tightly associated with the protein moiety of alpha-RNP and has developed secondary structure. The alpha-RNA is tightly associated with the protein moiety of alpha-RNP and has a high metabolic activity.
D000818:Animals; D002250:Carbon Radioisotopes; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism*; D003348:Cortisone / Q000031:analogs & derivatives* / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009963:Orotic Acid / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012261:Ribonucleoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D017411:Ribonucleoproteins, Small Nuclear; D014316:Tritium
D002250:Carbon Radioisotopes; D002843:Chromatin; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D014316:Tritium; D009963:Orotic Acid; D003348:Cortisone