Multiple forms of alkaline phosphatases in human liver tissue.
Alkaline phosphatases (AP) extracted in the presence of n-butanol from human liver are separated by affinity chromatography on phenylsepharose Cl-4B into two fractions named APII and APIIII. By repeated chromatography, APII was purified to a single enzyme entity with a specific activity of 1,684 kU/g protein. APIIII was purified to a specific activity of 535 kU/g protein. It consisted of only APIIII enzyme activity, but still contained gamma-glutamyltransferase activity. These two forms of AP are different in chromatographic and electrophoretic behaviour, APIIII being a larger molecule than APII. APII and APIIII are very similar in enzyme kinetic behavior, such as substrate activity, thermolability and sensitivity to different inhibitors. It is concluded from these experiments that multiple forms of AP in liver bear identical active centres, the difference is due to a modification of protein residue. It is possible that both are modified forms of one enzyme. Both are different from the AP isoenzyme that appears in serum in cholestatic patients.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D002779:Cholestasis / Q000201:enzymology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003545:Cysteine / Q000494:pharmacology; D004588:Electrophoresis, Cellulose Acetate; D006709:Homoarginine / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009439:Neuraminidase; D010649:Phenylalanine / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D007527:Isoenzymes; D006709:Homoarginine; D010649:Phenylalanine; D014508:Urea; D000469:Alkaline Phosphatase; D009439:Neuraminidase; D003545:Cysteine