[Binding of testosterone to plasma proteins in the viviparous male lizard].
Testosterone binding to plasma proteins has been analyzed in the viviparous lizard by electrophoresis at steady state conditions and by equilibrium dialysis. Two binding systems are involved. The first system (S1) binds estradiol and testosterone, it is Sex Binding Protein like. The second one binds testosterone and dihydrotestosterone; the mains competitors are C21 steroids: progesterone and cortisone; estradiol doesn't perturb the equilibrium; this system is Corticosteroid Binding Globulin like. Androstenedione doesn't seem to be bound by these two systems. The high affinity (KA 4 degrees C = 1.28 X 10(8) M-1) and the high capacity (N = 1,18 X 10(-5) mole/litre) suggest that it is the second system that supports the main transport, buffer, reservoir role in the blood of viviparous lizard.
D000818:Animals; D001667:Binding, Competitive; D007700:Kinetics; D008116:Lizards / Q000097:blood*; D008297:Male; D008970:Molecular Weight; D012738:Sex Hormone-Binding Globulin / Q000302:isolation & purification / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*
D012738:Sex Hormone-Binding Globulin; D013739:Testosterone
Liaison de la testostérone aux protéines plasmatiques chez le lézard vivipare mâle.