DNA-DNA and DNA-protein crosslinking and repair in Neurospora crassa following exposure to nitrogen mustard.
A new method was developed for the analysis of DNA-protein crosslinks in Neurospora crassa. The formations of DNA-protein and DNA-DNA crosslinks were assayed following exposure of spheroplasts to HN2. Both types of crosslink were detected and were found to be repaired during recovery. Moreover a mutant sensitive to HN2 was defective in the removal of both types of crosslink.
D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics*; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D008466:Mechlorethamine / Q000494:pharmacology*; D008770:Methylnitrosourea / Q000494:pharmacology; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D003852:Deoxyribonucleoproteins; D008466:Mechlorethamine; D008770:Methylnitrosourea