Major histocompatibility complex-restricted self-recognition in responses to trinitrophenyl-Ficoll. A novel cell interaction pathway requiring self-recognition of accessory cell H-2 determinants by both T cells and B cells.
In vitro primary antibody responses to limiting concentrations of trinitrophenyl (TNP)-Ficoll were shown to be T cell dependent, requiring the cooperation of T helper (TH) cells, B cells, and accessory cells. Under these conditions, TH cells derived from long-term radiation bone marrow chimeras were major histocompatibility complex (MHC) restricted in their ability to cooperate with accessory cells expressing host-type MHC determinants. The requirement for MHC-restricted self-recognition by TNP-Ficoll-reactive B cells was assessed under these T-dependent conditions. In the presence of competent TH cells, chimeric B cells were found to be MHC restricted, cooperating only with accessory cells that expressed host-type MHC products. In contrast, the soluble products of certain monoclonal T cell lines were able to directly activate B cells in response to TNP-Ficoll, bypassing any requirement for MHC-restricted self-recognition. These findings demonstrate the existence of a novel cell interaction pathway in which B cells as well as TH cells are each required to recognize self-MHC determinants on accessory cells, but are not required to recognize each other. They further demonstrate that the requirement for self-recognition by B cells may be bypassed in certain T-dependent activation pathways.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000955:Antigens, T-Independent / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology*; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D006462:Hemolytic Plaque Technique; D008285:Major Histocompatibility Complex; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*
D000955:Antigens, T-Independent; D006183:H-2 Antigens; D009578:Nitrobenzenes; D011134:Polysaccharides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll