A quantitative comparison of T-cell subsets in Hodgkin's disease and reactive hyperplasia. Frozen section immunohistochemistry.
Cryostat sections of lymph nodes from patients with Hodgkin's disease were stained with monoclonal antibodies reactive with T-cells including T-cell subsets. Lymph nodes showing reactive hyperplasia were used as controls. Using an eyepiece grid, the stained cells were counted and subset ratios determined. The ratio of helper T-cells to cytotoxic/suppressor T-cells, i.e., (formula; see text) in Hodgkin's disease was found to be significantly different from ratios obtained from reactive nodes. This difference may be explained by a relative increase in cytotoxic/suppressor T-cells and/or a decrease in helper T-cells. This finding may relate to the previously reported increase in suppressor cell activity in patients with Hodgkin's disease and may also correlate with diminished delayed-type hypersensitivity in such patients.
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D005629:Frozen Sections; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D006965:Hyperplasia / Q000276:immunology; D007158:Immunologic Techniques; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal