title: Rapid responses of lymphocytes in an optimized mixed lymphocyte culture.
abstract: When adherent mononuclear cells are removed from whole blood, the separated lymphocytes respond rapidly by proliferation to foreign cocultured lymphocytes. The reaction of responding cells against a pool of foreign stimulating cells can be detected in this rapid mixed lymphocyte culture (r-MLC) within 28 h and antigenic disparity between family members, differing for only one HLA haplotype, can be measured within 36 h. These early responses show genetic specificity corresponding to those observed in a standard MLC at 96 h. The involvement of similar cell populations in the regulation of early responses to allogeneic cells and increased responses to autologous cells in r-MLC, as well as accelerated responses in secondary MLC, is discussed.
mesh_terms: D002455:Cell Division; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007104:Immune Adherence Reaction; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D006680:HLA Antigens