[Properties of mouse spleen residual condensed chromatin associated with the nuclear matrix].
Properties of condensed residual chromatin of mouse spleen, a component of residual nuclear structures, were studied. Extraction of the structures with buffers of different NaCl concentrations showed that the condensed chromatin consists of condensed nucleosomal chains. On increasing the ionic strength the complexes gradually fell apart into separate nucleosomal chains. DNA of condensed chromatin was accessible to staphylococcal nuclease and DNAase I, but digestion of this DNA was not accompanied by solubilization of the residual chromatin. Besides the essentially decreased total content of nonhistone chromosomal proteins the condensed chromatin practically did not contain HMG proteins. The nucleosome repeat length of this chromatin was shorter than that of chromatin solubilized by staphylococcal nuclease.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002843:Chromatin / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000302:isolation & purification; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases; D051379:Mice; D008836:Micrococcal Nuclease; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification; D009707:Nucleosomes / Q000032:analysis; D009994:Osmolar Concentration; D013154:Spleen / Q000032:analysis*
D002843:Chromatin; D009698:Nucleoproteins; D009707:Nucleosomes; D004247:DNA; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease
Svoĭstva ostatochnogo kondensirovannogo khromatina selezenki myshi, assotsiirovannogo s iadernym matriksom.