Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): construction of his-lac gene fusions.
The bacteriophage Mu d1(Apr lac cts62 ) obtained from an Escherichia coli double lysogen carrying the defective Mu d1 phage and a Mu-P1 hybrid phage was utilized as a vector for phage mutagenesis in Erwinia carotovora subsp. carotovora. Among ampicillin-resistant transductants. 1.4% were auxotrophs. The synthesis of beta-galactosidase was derepressed upon starvation for histidine in two different his-lac fusion strains.
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis; D000667:Ampicillin / Q000494:pharmacology; D010583:Bacteriophage mu / Q000235:genetics*; D004274:DNA, Recombinant; D004790:Enzyme Induction; D004885:Erwinia / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D006639:Histidine / Q000096:biosynthesis*; D009154:Mutation; D009876:Operon; D010403:Penicillin Resistance; D014161:Transduction, Genetic
D004274:DNA, Recombinant; D006639:Histidine; D000667:Ampicillin; D000429:Alcohol Oxidoreductases; C021358:histidinol dehydrogenase