Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin.
A Bacillus subtilis strain, DSM704, was characterized by genetic means, and its production of a human intestinal sucrase inhibitor, 1-deoxynojirimycin, was described. Synthesis of this compound is detected concomitant with the detection of heat-resistant spores. The amount of 1-deoxynojirimycin produced is highly dependent on the carbon source, with growth on substrates metabolized via glycolysis giving the greatest amount of production (up to 1 mg/ml). 1-Deoxynojirimycin appears to be nonmetabolizable by the producing strain in that it cannot serve as a sole carbon or nitrogen source.
D017485:1-Deoxynojirimycin; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development*; D001435:Bacteriophages / Q000235:genetics; D003470:Culture Media; D005944:Glucosamine / Q000031:analogs & derivatives / Q000096:biosynthesis; D007700:Kinetics; D013393:Sucrase / Q000037:antagonists & inhibitors; D014161:Transduction, Genetic
D000900:Anti-Bacterial Agents; D003470:Culture Media; D017485:1-Deoxynojirimycin; D013393:Sucrase; D005944:Glucosamine