The simultaneous electrophoretic analysis of esterase D and phosphoglucomutase subtyping in fresh blood and in dried bloodstains.
Phosphoglucomutase1 (PGM) subtyping and esterase D phenotyping were simultaneously performed by electrophoresis of bloodstained fibers using agarose and a Tris-maleic acid buffer system , pH 5.4. This method reduces anodal gel shrinkage and shortens development time when compared to the conventional electrophoretic technique for PGM subtyping which is performed at pH 7.4 using an agarose-starch substrate.
D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D010733:Phosphoglucomutase / Q000097:blood*
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D010733:Phosphoglucomutase