Distribution of membrane-associated actin in sheep lymphocytes. Functional implications.
Using the DNAase I inhibition assay, we titrated two forms of actin, monomeric (G) and total (G + F) actin of various populations of lymphocytes in adult and fetal sheep. Lymphocytes leaving a lymph node always showed higher actin activities than lymphocytes resident in the lymph node at the time of examination. T cells had significantly higher actin activities than B cells. A subpopulation of T cells which is present in the blood but not in the lymph showed much lower activities than total T cell populations in the blood or efferent lymph. Thymocytes showed the lowest actin activities among the lymphoid cell populations examined. A proposal is made that DNAase I inhibitions per cell can be considered as an intrinsic parameter of lymphocyte recirculating abilities.
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology; D002352:Carrier Proteins / Q000032:analysis*; D002469:Cell Separation; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors; D005260:Female; D018260:Gelsolin; D008196:Lymph / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008840:Microfilament Proteins; D012756:Sheep; D013499:Surface Properties; D013601:T-Lymphocytes / Q000145:classification
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D018260:Gelsolin; D008565:Membrane Proteins; D008840:Microfilament Proteins; C016953:brevin; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I