In vivo collaboration between precursor T cells and helper T cells in the development of delayed-type hypersensitivity reaction to influenza virus in mice.
Nude, athymic mice do not mount a delayed-type hypersensitivity (DTH) response to influenza A virus. A single injection of T helper cells (gamma-irradiated, 2-day immune spleen cells) or three injections over 3 days of a concanavalin-A-activated spleen cell supernatant to virus-sensitized nude mice resulted in a 'normal' DTH response when the mice were challenged with the virus. It was previously shown that the cells responsible for the reaction were T cells and required I-region compatibility. Injection of T helper cells into normal mice did not affect the level of the subsequent DTH response. However, injection of such cells into mice pretreated with anti-thymocyte serum (ATS) restored the ability of the mice to mount a DTH response. The results show that (1) nude mice contain precursor T cells for influenza virus antigen; (2) an I region-restricted response can be generated in the absence of a thymus; and (3) in vivo collaboration between DTH T-cell precursors and helper T cells can be shown to occur in congenitally nude mice and ATS-treated mice.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive; D009980:Influenza A virus / Q000276:immunology; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009976:Orthomyxoviridae Infections / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000528:radiation effects; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000961:Antilymphocyte Serum