In vitro production of gamma interferon is dependent on the mouse genotype.
C57B1/6 and BALB/c mice were compared for their in vitro capacity to produce gamma interferon (IFN-gamma) in response to specific antigen (i.e., Purified Protein Derivative in bacillus Calmette-Guérin sensitized animals) and T-cell mitogen (Concanavalin A). In both cases C57B1/6 spleen cells produced five to ten times more IFN than identically treated BALB/c spleen cells. More detailed analysis of in vitro induced BCG-PPD IFN indicates that the low IFN level in BALB/c cultures is neither caused by classical splenic suppressor cells such as macrophages or T-cells, nor by inactivation of secreted IFN by inhibitory substances.
D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology; D002448:Cell Adhesion; D003208:Concanavalin A / Q000494:pharmacology; D005838:Genotype; D007158:Immunologic Techniques; D007369:Interferon Inducers / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008810:Mice, Inbred C57BL / Q000235:genetics*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014373:Tuberculin / Q000276:immunology
D001500:BCG Vaccine; D007369:Interferon Inducers; D014373:Tuberculin; D003208:Concanavalin A; D007371:Interferon-gamma