Molecular cloning and characterization of DNA sequences encoding rat and human atrial natriuretic factors.
A cDNA copy of the message encoding rat atrial natriuretic factor (ANF) has been cloned in Escherichia coli, and its nucleotide sequence was determined. ANF appears to be synthesized as a larger precursor, atrial pronatriodilatin. The cDNA has an open reading frame potentially encoding a protein of 152 amino acids, of which the first 24 amino acids strongly resemble a signal sequence. This is followed by a sequence with 80% homology to a second vasoactive protein, porcine cardiodilatin. The ANF peptide is contained in the COOH-terminal portion of the protein. The DNA sequence corresponding to human ANF is also presented and displays a high degree of homology to its rat counterpart. These data provide further evidence for the expression in cardiac atria of a multifactor system that may contribute to the regulation of blood pressure and extracellular fluid volume.
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics*; D009318:Natriuresis; D045283:Natriuretic Agents; D011498:Protein Precursors / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D009124:Muscle Proteins; D045283:Natriuretic Agents; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D004247:DNA