Electron microscopic evidence for the transmembrane displacement of calcium ATPase.
Incubation of the Ca2+-ATPase in vanadate solutions leads to the formation of two-dimensional arrays in the sarcoplasmic reticulum membrane. Electron micrographic freeze fracture replicas show depressions on the inner leaflet for the first time. This indicates that the ATPase has moved perpendicular to the plane of the membrane. Our results also suggest that aggregation of the Ca2+-ATPase into the two-dimensional arrays occurs before they move into the membrane. These phenomena were observed as soon as 15 minutes after vanadate was added. The effects of vanadate appear to be completely reversible. When SR was incubated in the vanadate solutions and was then diluted into a buffer containing Ca2+ and ATP, the ATPase activity was normal for up to several hours of incubation and only somewhat reduced after 3 days.
D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005614:Freeze Fracturing; D006801:Humans; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D014639:Vanadium; D014638:Vanadates; D000252:Calcium-Transporting ATPases