Characterization of two suppressor cells that together prevent in vivo development of cytolytic T cells to hapten-altered self.
Two suppressor cell populations that interact to down-regulate in vivo development of the cytolytic T-cell (CTL) response to trinitrophenyl-modified syngeneic spleen cells (TNP-SC) have been further characterized. Suppressor cells induced by the iv injection of trinitrophenyl-modified syngeneic spleen cells possess Thy 1.2 antigen. Their precursors are insensitive to pretreatment of host animals with cyclophosphamide (CY). Suppressor cells that arise after dermal sensitization with trinitrochlorobenzene are also Thy 1.2 antigen positive but their precursors are sensitive to pretreatment with CY. These characteristics of the two suppressor T cells (Ts) are identical to those of the two Ts that are generated by similar methodologies and that together suppress contact sensitivity (CS) to picryl chloride. Neither the CS nor CTL response was suppressed when host animals possessed only one set of Ts. In contrast to suppression of CS at the efferent phase, development of CTL was suppressed only when the two Ts were present early during sensitization (afferent phase). Since the results point to several similarities between the two sets of Ts that are active in the down-regulation of the CS and CTL responses, it is suggested that the two dissimilar immune responses directed to the same hapten, namely CS and CTL, may be controlled by the same suppressor cells. Since it appears that the two sets of Ts interact to affect different phases of the CS and CTL responses, down-regulation of each must be accomplished through different mechanisms.
D000818:Animals; D002450:Cell Communication; D003602:Cytotoxicity, Immunologic; D003877:Dermatitis, Contact / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D007108:Immune Tolerance; D007116:Immunization, Passive; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D006241:Haptens; D014301:Trinitrobenzenes