Infectious poxvirus vectors have capacity for at least 25 000 base pairs of foreign DNA.
To test the capacity of poxviruses for added foreign DNA, a recombinant was constructed that contains 24 700 bp of bacteriophage lambda DNA inserted within the vaccinia virus thymidine kinase (TK) gene. The recombinant is stable, infectious and replicates in tissue culture at the same rate and to the same titer as standard vaccinia virus. This size flexibility of the poxvirus genome and the lack of stringent packaging requirements are useful features for an infectious eukaryotic cloning vector.
D010582:Bacteriophage lambda / Q000235:genetics; D001482:Base Composition; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D011212:Poxviridae / Q000235:genetics*; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics; D010948:Viral Plaque Assay
D004279:DNA, Viral; D013937:Thymidine Kinase