title: Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli.
abstract: 1. Neurospora crassa catabolic dehydroquinase has been purified from N. crassa and Escherichia coli. 2. Protein-sequence and gel-electrophoretic data show that apparently pure, homogeneous native dehydroquinase is a mixture of intact and proteinase-cleaved enzyme monomers. 3. Protein-sequence data and steady-state kinetics show that the catabolic dehydroquinase gene of N. crassa is expressed with fidelity in E. coli.
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000201:enzymology*; D006836:Hydro-Lyases / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*
chemical_list: D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase