The effect of C4 on C1 binding and activation.
Cell-bound human C4 enhances the uptake of C1 by immunoglobulin-carrying cells. The effect is more pronounced with small amounts of IgG than with large amounts. Furthermore, the presence of C4 on the cell surface dramatically promotes the enzymatic effect of C1 on C2. The C1 binding is efficiently blocked, and the C1 activation is almost abolished by pretreating IgG-bearing cells with protein A from Staphylococcus aureus. In contrast, protein A has no effect on cells carrying C4 in addition to IgG. Protein A added in small amounts to IgG-coated sheep erythrocytes inhibits the haemolysis by subsequently added human serum, whereas even larger amounts of protein A has no effect when added to cells carrying C4 as well. Cells with and without C4 take up protein A equally well.
D000818:Animals; D003167:Complement Activation / Q000187:drug effects; D003172:Complement C1 / Q000378:metabolism*; D003181:Complement C4 / Q000378:metabolism*; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D011951:Receptors, Complement / Q000187:drug effects; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000187:drug effects; D013205:Staphylococcal Protein A / Q000378:metabolism / Q000494:pharmacology
D003172:Complement C1; D003181:Complement C4; D011951:Receptors, Complement; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D013205:Staphylococcal Protein A