Identification of donor-derived antigen-specific suppressor cells in murine bone marrow chimeras prepared with total-lymphoid irradiation.
The suppressor activity of the spleen cells from bone marrow chimeras prepared with total-lymphoid irradiation was analyzed in vitro. The chimeric spleen cells lacked responsiveness to host-type, but not to third-party, antigens in the mixed-leukocyte reaction (MLR) as judged by (3H)thymidine incorporation and the generation of cytolytic cells. When the donor-type chimeric spleen cells were used as cocultured cells in the MLR, modest nonspecific suppression of (3H)thymidine incorporation and potent antigen-specific suppression of the generation of the cytolytic cells was observed. The donor-type suppressor cells may play an important role in preventing graft-versus-host disease in vivo.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D002860:Chromium Radioisotopes; D007959:Lymphocyte Culture Test, Mixed; D008221:Lymphoid Tissue / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014916:Whole-Body Irradiation
D002860:Chromium Radioisotopes