title: A method to increase the volume of sample applied to isoelectric focusing gels.
abstract: Isoelectric focusing of extracts from diluted or aged bloodstains may be more successfully accomplished with larger sample volumes applied to the gel. A technique is described using teflon tubing to apply larger sample volumes (up to 100 microliters) to isoelectric focusing (IEF) gels. This method is reproducible and easy to perform.
mesh_terms: D001801:Blood Stains; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D010733:Phosphoglucomutase / Q000032:analysis* / Q000235:genetics
chemical_list: D010733:Phosphoglucomutase