Calcium-activated, phospholipid-dependent protein kinase in pig epidermis.
We investigated calcium-activated, phospholipid-dependent protein kinase in pig epidermis. Pig epidermal homogenates were centrifuged at 30,000 g for 30 min, and the supernatant was applied on a DEAE-cellulose column for purification. The partially purified enzyme was stimulated by simultaneous addition of Ca2+ and phospholipid. Successive addition of small amounts of diolein further activated the enzyme activity. The calcium-activated phospholipid-dependent protein kinase preferentially phosphorylated serine residues and its endogenous substrate protein in the pig epidermis has a molecular weight of about 97,000.
D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D010743:Phospholipids / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D012867:Skin / Q000201:enzymology*; D013552:Swine
D010743:Phospholipids; D011494:Protein Kinases; D011493:Protein Kinase C; D002118:Calcium