Rapid method for the simultaneous analysis of hydrocortisone and clioquinol in topical preparations by high-performance liquid chromatography.
Reversed-phase high performance liquid chromatographic methods for the analysis of ointments containing hydrocortisone and clioquinol have been investigated. A successful method using a C18 column and methanol--0.05 M phosphoric acid (80:20) as eluting solvent has been developed which allows both compounds to be determined simultaneously. The high-performance liquid chromatographic procedure is rapid and sensitive whereas the assay described in the 1980 BP involves a different method for the analysis of each component of the ointment. The method has also been further applied to the analysis of ointments containing hydrocortisone combined with other halogenated hydroxyquinolines.
D002851:Chromatography, High Pressure Liquid; D007464:Clioquinol / Q000032:analysis*; D004338:Drug Combinations; D006854:Hydrocortisone / Q000032:analysis*; D006912:Hydroxyquinolines / Q000032:analysis*; D009824:Ointments / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D004338:Drug Combinations; D006912:Hydroxyquinolines; D009824:Ointments; D007464:Clioquinol; D006854:Hydrocortisone