Glucose-induced accumulation of fructose-2,6-bisphosphate in pancreatic islets.
Rat islets contain the acid-labile activator of phosphofructokinase, fructose-2,6-bisphosphate. The islet content in activator is higher in islets exposed to glucose (16.7 mM) than in islets deprived of glucose. The islets display fructose-6-phosphate, 2-kinase activity with a Km for fructose-6-phosphate close to 0.08 mM. Glucose fails to affect the activity of this enzyme. It is proposed that the effect of glucose to increase the islet content of fructose-2,6-bisphosphate is attributable, in part at least, to the glucose-induced increase in the concentration of fructose-6-phosphate in the islet cells.
D000818:Animals; D004789:Enzyme Activation; D005635:Fructosediphosphates / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000378:metabolism*; D007515:Islets of Langerhans / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000502:physiology; D051381:Rats
D005635:Fructosediphosphates; D005636:Fructosephosphates; D006598:Hexosediphosphates; C027618:fructose-6-phosphate; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1; D005947:Glucose