The relationship of lytic effects against autologous and allogeneic PHA blasts induced in human mixed lymphocyte cultures.
In addition to the cytotoxic T lymphocytes (CTL) killer cells which lyse autologous PHA lymphoblasts are also generated in mixed lymphocyte cultures (MLC). By modifying various tissue culture conditions, we demonstrate that the generation of allospecific effectors can be reduced but not the lytic activity against autologous PHA lymphoblasts. Cold target inhibition experiments showed a cross reaction between autologous and allogeneic PHA lymphoblasts. No inhibition was seen with fresh lymphocytes. It seems, therefore, that the measured lytic activity of specific CTL generated in a MLC is in fact partially mediated by cytotoxic cells which kill also autologous PHA lymphoblasts.
D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins