Bromobimane crosslinking studies in oligomycin-sensitive ATPase from beef heart mitochondria. Mr 31 000 protein crosslinked.
Using a bromobimane fluorescent label the Mr 31 000 protein band oligomycin-sensitive (OS)-ATPase from beef heart mitochondria is shown to become much intensified by 2-mercaptopropionylglycine. In the presence of 3.5 nmol/mg protein of the thiol reagent ATP-Pi exchange activity is increased by 90%. With the fluorescent crosslinking reagent dibromobimane (DB) we show that a new fluorescent peak appears between Mr 50 000 and 60 000. ATP-Pi exchange is very much decreased by DB. The results suggest that for regulation of ATP-synthetase activity sulfhydryl groups in the region of the Mr 31 000 protein(s) play an important role.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D002417:Cattle; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D008929:Mitochondria, Heart / Q000201:enzymology*; D008970:Molecular Weight; D009840:Oligomycins / Q000494:pharmacology
D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D009840:Oligomycins; C037094:dibromobimane; D000251:Adenosine Triphosphatases; C028381:monobromobimane