Antibodies to chromosome 21 coded cell surface components block binding of human alpha interferon but not gamma interferon to human cells.
Antisera raised against a human X mouse hybrid cell line containing human chromosome 21 as its only human chromosome, block induction of an antiviral state by human alpha interferon (IFN-alpha), block induction of (2'-5')oligoisoadenylate synthetase [2'-5')A synthetase), and block binding of 125I-labeled and 35S-labeled recombinant, human IFN-alpha A, but not 125I-labeled IFN-gamma, to cell surface receptors. The data presented clearly demonstrate that the cell surface receptors for IFN-alpha and IFN-gamma are different, and provide independent evidence of the role of a chromosome 21 coded cell surface molecule in the pathway to the generation of the antiviral state.
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D000936:Antigen-Antibody Complex; D002904:Chromosomes, Human, 21-22 and Y; D004790:Enzyme Induction; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007106:Immune Sera; D007370:Interferon Type I / Q000378:metabolism* / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007370:Interferon Type I; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase