In vivo kinetics and nature of rat IgE-bearing lymphocytes after IgE stimulation.
Surface IgE-bearing (sIgE+) cells were studied in BN and rnu/rnu athymic rats after Nippostrongylus brasiliensis infection or i.p. injection of unpurified or purified IgE from plasmacytoma ascitic fluid. The number of sIgE+ cells increased markedly after a serum IgE increase without change in the proportion of sIgM+ and sIgD+ cells. A high percentage of the sIgE+ cells bore cytophilic IgE. Receptors for IgE were induced with a 2.56-micrograms IgE injection/100 g body weight and reached a maximum with 1.6 mg IgE/100 g body weight. They appeared less than 4 hr after a single injection of purified IgE. The number of IgE receptor-bearing cells reached a maximum plateau at 24 hr to day 3 after injection and declined thereafter, to reach the control level on day 9 or 11 after injection. Nearly all the sIgE+ cells of BN rats also bore sIgD, but the number of triple sIgE-sIgM-sIgD+ cells varied in a wide range. Maximum 4.5% of the sIgE+ cells of euthymic rats were T cells. More than 98% of the sIgE+ cells of nude rats were triple sIgM-sIgD-sIgE+ cells, and the majority were cytophilic IgE+. For the most part, the sIgM-sIgD-sIgE+ cells are probably not cells that can differentiate, as generally accepted, in IgE-producing cells. New interpretations of the role of these triple sIgM-sIgD-sIgE+ cells in IgE immune responses are necessary.
D000818:Animals; D001854:Bone Marrow Cells; D003593:Cytoplasm / Q000276:immunology; D005260:Female; D006725:Hookworm Infections / Q000276:immunology; D007073:Immunoglobulin E / Q000008:administration & dosage / Q000378:metabolism* / Q000502:physiology; D007700:Kinetics; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011922:Rats, Mutant Strains; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis; D013154:Spleen / Q000166:cytology
D011947:Receptors, Antigen, B-Cell; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D007073:Immunoglobulin E