1H-NMR study of the lambda operator site OL1: assignment of the imino and adenine H2 resonances.
One- and two-dimensional proton NMR methods are being used to study the synthetic lambda operator site O-L1, a 17 base-pair DNA duplex recognized by lambda repressor and Cro protein. The complete assignment of the 17 imino protons, which participate in Watson-Crick hydrogen bonding, and of the eight adenine H2 protons, which lie in the minor groove of the double helix, is presented.
D000225:Adenine; D010582:Bacteriophage lambda / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009876:Operon
D004279:DNA, Viral; D000225:Adenine