Regulatory mechanisms in cytotoxic T lymphocyte development. I. A suppressor T cell subset that regulates the proliferative stage of CTL development.
The alloantigen-induced suppressor function of cells from 3-day mixed leukocyte culture (MLC) was studied. These cells, when co-cultured with normal syngeneic lymphocytes and cells of the same haplotype as the original inducing alloantigen, inhibited the generation of cytotoxic T lymphocytes (CTL). Suppression was mediated by a radiation-resistant Lyt-2+ T cell. The suppressor T cells appeared to act by inhibiting the clonal expansion of CTL precursors in the responder cell population, determined by limiting dilution analysis. Levels of endogenous interleukin 2 (IL 2) in co-cultures with suppressor T cells were diminished, and the addition of exogenous IL 2 to co-cultures cancelled the suppressor T cell effects. The suppressor cell population was shown to be capable of absorbing IL 2 from lymphokine preparations, and in contrast to mitogen-induced suppressor T cells, after exposure to IL 2 the allostimulated suppressor T cell remains active. The results are discussed in terms of possible modes of action of the suppressor T cell.
D000042:Absorption; D000818:Animals; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism
D007376:Interleukin-2