Indirect immunofluorescent antibody test in chicken leucocytozoonosis.
The indirect immunofluorescent antibody technique was applied to detection of antigens of different developmental stages of Leucocytozoon caulleryi and antibodies in the sera of chickens infected with L. caulleryi by using second generation merozoite and gametocyte antigens. Zygotes, ookinetes and sporozoites in midges, and second generation merozoites and gametocytes in chickens indicated specific fluorescence. Indirect immunofluorescent antibody titers were higher than agar gel precipitation antibody titers. So the detection of the antibody by the indirect immunofluorescent antibody technique was possible in the sera in which the antibody could not be detected by the agal gel precipitation test. Therefore, the indirect immunofluorescent antibody technique was applicable to chicken leucocytozoonosis as a highly sensitive serological diagnostic method.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000032:analysis; D016782:Apicomplexa / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis*; D011528:Protozoan Infections / Q000175:diagnosis; D011529:Protozoan Infections, Animal; D013047:Specific Pathogen-Free Organisms
D000906:Antibodies; D000941:Antigens