title: The semi-quantitative distribution of T4 and T6 surface antigens on human Langerhans cells.
abstract: We have used indirect immunogold electron microscopy to compare the respective density of cell membrane determinants revealed by OKT6 and OKT4 monoclonal antibodies on normal human Langerhans cells (LC): 12.9 +/- 3.5 gold granules were noted per cell section on OKT4-positive LC whereas 236.8 +/- 23.5 granules were counted per cell section on OKT6-reactive cells. These results confirm that human LC react with OKT4 antibody and they demonstrate a marked quantitative difference on LC surface between the antigenic determinants recognized by OKT6 and OKT4 antibodies.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D000939:Epitopes / Q000032:analysis; D006046:Gold; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D006046:Gold