Immune responsiveness against the human placenta. II. Response with xenogeneic lymphocytes in culture.
Human placental plasma membrane vesicles were cultured for up to 5 days in the presence of spleen cells from (BALB/c X C57BL/6By) F1 hybrid mice. The membrane preparations either inhibited the uptake of [3H]-thymidine ([3H]-TdR) by destroying the viability of the T-cell population or stimulated weak lymphocyte division which was primarily an expansion of the T-cell population. This differential effect was dependent on the membrane concentration in culture and the length of time the membrane preparation had been stored. Membrane preparations that inhibited [3H]-TdR uptake could be converted into stimulatory-type membranes by preincubating them at 37 degrees for several days. This conversion coincided with a change in status concerning membrane susceptibility to disruption by cytotoxic non-T-cells present in the spleen of unimmunized animals. The conversion from stimulating-type membrane into inhibitory-type was never observed. Throughout these cultures the generation of cell-mediated cytolysis could not be detected.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008871:Microvilli / Q000276:immunology; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D003208:Concanavalin A