Subsets of blood, spleen and recirculating lymphocytes in man.
Lymphocyte subpopulations were characterized in human blood and spleens. In addition the spleens were perfused by a closed extracorporeal perfusion system under almost physiological conditions. Lymphocytes released from the spleen during perfusion were taken to be representative of recirculating lymphocytes. B lymphocytes were classified by their surface immunoglobulin, T lymphocytes and T lymphocyte subsets by cytochemistry, sheep red blood cell rosette formation and in some experiments by monoclonal antibodies. In the blood 71 +/- 4.3% of the lymphocytes were rosette forming cells and 23.3 +/- 3.8% B lymphocytes. In the spleen 49.8 +/- 3.6% were T and 53.3 +/- 2.1% were B lymphocytes. In three spleens the mean number of OKT3+ lymphocytes were 27.6 +/- 7.0% OKT4+ 8.6 +/- 1.4% and OKT8+ 13.7 +/- 2.2%. The ratio of T helper to T suppressor lymphocytes was 0.67 for the spleen and 1.7 for the blood. The lymphocytes released from the perfused spleen showed a similar distribution pattern of surface markers to that of the splenic subpopulations.
D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000145:classification*; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000032:analysis; D010477:Perfusion; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012397:Rosette Formation; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000145:classification; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory
D000911:Antibodies, Monoclonal; D011947:Receptors, Antigen, B-Cell; D009283:Naphthol AS D Esterase