Carminic acid, a non-competitive inhibitor of kidney UDP-glucose:galactosylhydroxylysine-collagen glucosyltransferase.
1. UDP-glucose:galactosylhydroxylsine-collagen glucosyltransferase was purified 12-fold from rat kidney. 2. An assay using calf-skin gelatin as substrate showed time- and enzyme-dependent incorporation; KmS for UDP-glucose and gelatin were 16-7 microM and 4.5 mg/ml, respectively. 3. Column chromatography of the alkaline hydrolysate of reaction product on Dowex 50W-4X(H+) showed that 84% of the radioactivity was in the glycosylgalactosylhydroxylsine peak. 4. Carminic acid inhibited collagen glycosyltransferase; a dose-dependent study showed a two-stage inhibition and kinetic analysis by double-reciprocal plots at varying UDP-glucose concentrations revealed a non-competitive mode of inhibition.
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D002329:Carmine / Q000031:analogs & derivatives / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D007668:Kidney / Q000201:enzymology; D008297:Male; D051381:Rats
D000880:Anthraquinones; D003094:Collagen; D002329:Carmine; D005964:Glucosyltransferases; C023247:UDP glucose-collagen glucosyltransferase