Biosynthesis of proteokeratan sulfate in the bovine cornea. 1) Isolation and characterization of a keratan sulfotransferase and the role of sulfation for the chain termination.
D000818:Animals; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis*; D002848:Chromatography, DEAE-Cellulose; D003315:Cornea / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007632:Keratan Sulfate / Q000096:biosynthesis*; D007700:Kinetics; D000071234:Lumican; D008861:Microsomes / Q000201:enzymology; D010443:Peptide Chain Termination, Translational; D011509:Proteoglycans / Q000096:biosynthesis*; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000302:isolation & purification*; D013696:Temperature; D000097811:Carbohydrate Sulfotransferases
D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D007632:Keratan Sulfate; D000071234:Lumican; D011509:Proteoglycans; D015238:Sulfotransferases; D013466:Sulfurtransferases; D000097811:Carbohydrate Sulfotransferases; C019893:chondroitin 4-sulfotransferase