Characterization of a factor inducing differentiation of mouse myeloid leukemic cells purified from conditioned medium of mouse Ehrlich ascites tumor cells.
A factor inducing differentiation of mouse myeloid leukemic cells (MI) into macrophages was purified to apparent homogeneity from 168 1 of CM of Ehrlich ascites tumor cells. The purified factor was half-maximally active at 2 X 10(-11) M. The factor was analyzed by radioiodination, SDS-polyacrylamide gel electrophoresis and autoradiography. Its Mr was 40 000-50 000. On reduction, the factor lost activity, but showed no subunit structure. Treatment of the factor with endo-beta-N-acetylglucosaminidase F, but not endo-beta-N-acetylglucosaminidase H, gave rise to a molecule of Mr 20 000-28 000. The activity of the factor from Ehrlich cells was completely neutralized by antiserum to the factor of Mr 50 000-70 000 from mouse fibroblast L929 cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006131:Growth Inhibitors; D007106:Immune Sera / Q000276:immunology; D015850:Interleukin-6; D007739:L Cells / Q000032:analysis; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid / Q000473:pathology*; D008222:Lymphokines; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight
D000939:Epitopes; D006023:Glycoproteins; D006131:Growth Inhibitors; D007106:Immune Sera; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D006026:Glycoside Hydrolases