Two-dimensional electrophoresis of plasma membranes, showing differences among wild-type and abnormal ascospore mutant strains of Neurospora crassa.
Plasma membranes isolated from vegetative cultures of wild-type Neurospora crassa were analyzed by two-dimensional electrophoresis, followed by staining with silver nitrate to visualize proteins and fluorescein-labeled concanavalin A to visualize glycosylated subunits. Mycelial plasma membranes from strains carrying mutations affecting ascospores were also analyzed. Two of the mutant strains were shown to have aberrant two-dimensional membrane subunit patterns. The correlation of these abnormalities with the known electron microscopic evidence for aberrations of their ascospore-delimiting membrane during ascospore genesis is discussed.
D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D009154:Mutation; D009491:Neurospora / Q000032:analysis*; D009492:Neurospora crassa / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D013172:Spores, Fungal
D005656:Fungal Proteins; D006023:Glycoproteins; D008565:Membrane Proteins