Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.
A mouse hybrid cell line secreted monoclonal antibody which reacted specifically with Streptococcus mutans 6715 (serotype g) glucosyltransferase (GTase)-synthesizing water-insoluble glucan and inhibited with enzyme reaction. The antibody was cross-reactive with GTase of serotype d but not with GTase of other serotypes of S. mutans when an enzyme-linked immunosorbent assay was used.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D003429:Cross Reactions; D005964:Glucosyltransferases / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping; D013295:Streptococcus mutans / Q000201:enzymology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D005964:Glucosyltransferases