In vivo regulation of the cytolytic T cell response to hapten-altered self: suppressor T cells induced in the regional lymph nodes by exposure to syngeneic spleen cells.
During the course of examining the in vivo development of the cytotoxic T lymphocyte (CTL) response to hapten-modified self antigens in mice, we have observed that it can be abrogated through prior exposure of the host's regional lymph nodes (LN) to normal syngeneic spleen cells (NSC). Suppression appeared to be antigen nonspecific and was best seen when animals were injected in the footpads with NSC at least 72 h before sensitization for CTL. The ability to prevent the development of the CTL response was restricted primarily to syngeneic splenic T and B cells since syngeneic thymocytes stimulated only partial suppression and syngeneic LN cells, as well as hapten-coupled syngeneic spleen cells, did not at all. Suppression appeared to be a local phenomenon in that the NSC induced transferable suppressor T cells to appear in the popliteal LN draining the footpads but not in spleens. In addition, animals splenectomized prior to injections of NSC showed abrogated CTL responses equivalent to sham-splenectomized animals indicating that the spleen does not contribute to the mechanism of suppression. Finally, when only one footpad was injected with NSC, suppression was seen in the draining popliteal node and not in the contralateral node. Taken together, the evidence suggests that the source of the suppression and the suppressor T cells may be attributed to an in vivo syngeneic mixed lymphocyte reaction occurring between responder cells of the draining LN and injected stimulator spleen cells.
D000818:Animals; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D005260:Female; D006241:Haptens; D007165:Immunosuppression Therapy; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000637:transplantation; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D006241:Haptens