The ultrastructure of cat fusimotor endings and their relationship to foci of sarcomere convergence in intrafusal fibres.
1. Six muscle spindle poles, five from experiments in which foci of sarcomere convergence had been observed during stimulation of fusimotor axons, were serially sectioned for light and electron microscopy. Every somatic motor terminal was studied in ultrathin sections at several levels.2. In all six poles static gamma axons, or presumed static gamma axons, supplying the static bag(2) fibre and/or chain fibres had no terminations on the dynamic bag(1) fibre. In five poles, the dynamic bag(1) fibre was selectively innervated by dynamic gamma or beta axons save in one case where a dynamic gamma axon also innervated one chain fibre.3. Seventy-seven motor endings were of four distinct ultrastructural types: ;m(a) plates' lay superficially on the surface of static bag(2) or chain fibres; ;m(b) plates' were deeply indented into dynamic bag(1) fibres; in ;m(c) plates', found on chain fibres only, the muscle surface was thrown into projecting fingers between which the axon terminals were embedded; one type ;m(d) plate' was found, fully indented into a long chain fibre. A few plates of intermediate form (m(ab)) were variants of m(a) and m(b) plates.4. The muscle membrane beneath both m(a) and m(b) plates was smooth, or had a few wide, shallow folds; m(c) plates usually had wide, shallow subjunctional folds; numerous deep, narrow folds were characteristic of the m(d) plate. The length of unmyelinated pre-terminal axon or the number of sole plate nuclei were not useful diagnostic features.5. Obvious foci of sarcomere convergence in the capsular sleeve region of dynamic bag(1) and static bag(2) fibres coincided with the location of motor plates. Additional contraction foci were observed in the extracapsular region of dynamic bag(1) fibres where there was no motor innervation; contraction occurs principally in the outer half of these fibres. No foci of contraction or motor plates were observed in the extracapsular region of static bag(2) fibres; contraction in these fibres is typically mid-polar.6. In some poles local contraction of chain fibres centred on the location of m(c) plates. In others, very localized contraction occurred distal to the sites of m(a) plates. Both m(a) and m(c) plates were never found on the same pole of a chain fibre.7. Dynamic gamma or beta axons end in m(b) plates, probably equivalent to p(2) plates. The concept of distinctly different p(1) and p(2) plates on dynamic bag(1) fibres, supplied by dynamic beta and gamma axons, respectively, is not supported by ultrastructural evidence.8. Some static gamma axons end in multiple m(a) plates which correspond with ;trail endings', or in single large m(a) plates, on static bag(2) or chain fibres. The m(c) plates are the terminations of other static gamma, or occasionally dynamic gamma, axons on chain fibres. Static beta axons probably end in m(d) plates on long chain fibres which may correspond with p(1) plates.9. It is proposed that there are two types of static gamma motoneurone, one terminating in m(a) plates and the other in m(c) plates, possibly directed preferentially towards static bag(2) fibres and chain fibres, respectively.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002415:Cats; D003599:Cytoskeleton / Q000648:ultrastructure; D008854:Microscopy, Electron; D009045:Motor Endplate / Q000648:ultrastructure*; D009046:Motor Neurons / Q000648:ultrastructure*; D009047:Motor Neurons, Gamma / Q000648:ultrastructure*; D009470:Muscle Spindles / Q000648:ultrastructure; D009132:Muscles / Q000648:ultrastructure; D009210:Myofibrils / Q000648:ultrastructure*; D009469:Neuromuscular Junction / Q000648:ultrastructure*; D013570:Synaptic Membranes / Q000648:ultrastructure