Phylogenetic distribution of a 24,000 dalton human leukemia-associated antigen on platelets and kidney cells.
The distribution of a 24,000-dalton human leukemia-associated antigen, p24, was examined using the BA-2 and DU-ALL-1 monoclonal antibodies. BA-2 and DU-ALL-1 bound to human, gorilla, orangutan, macaque, and rabbit platelets but did not bind to mouse, rat, guinea pig, dog, horse, sheep, or goat platelets. Orangutan platelets demonstrated a decreased level of binding with BA-2 and DU-ALL-1. In addition, BA-2, but not DU-ALL-1, bound to chimpanzee platelets suggesting that the chimpanzee has lost the epitope of p24 detected by DU-ALL-1. Immunoperoxidase analysis of kidney tissue with BA-2 and DU-ALL-1 revealed staining of distal tubules and glomeruli, which occurred in a similar phylogenetic distribution to that of p24 on platelets. A monoclonal antibody to the high molecular weight common ALL antigen, J-5, reacted with glomeruli and proximal tubules from human, chimpanzee, orangutan, mangaby , rhesus, and rabbit kidneys but failed to react with rat or mouse kidney.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D001188:Artiodactyla / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D006736:Horses / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology*; D015260:Neprilysin; D010802:Phylogeny; D011323:Primates / Q000276:immunology; D012377:Rodentia / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015260:Neprilysin