An actin-depolymerizing protein (depactin) from starfish oocytes: properties and interaction with actin.
Physico-chemical properties and interaction with actin of an actin-depolymerizing protein from mature starfish oocytes were studied. This protein, which is called depactin, exists in a monomeric form under physiological conditions. Its molecular weight is approximately 20,000 for the native protein and approximately 17,000 for denatured protein. The Glu + Asp/Lys + Arg molar ratio of this protein is 1.55. The apparent pl of the denatured depactin is approximately 6. The extent of actin polymerization is reduced by the presence of depactin; however, the rate of polymerization seems to be accelerated as measured spectrophotometrically at 238nm. This effect is interpreted to indicate that depactin cut the newly formed filaments into small fragments, thereby increasing the number of the filament ends to which monomers are added. The apparent critical concentration of actin for polymerization, as determined by viscometry or flow birefringence measurement, is increased by the presence of depactin in a concentration-dependent manner. Raising the pH of the solution does not reverse the action of depactin. The molar ratio of actin and depactin, which interact with each other, is estimated to be 1:1 by means of a cross-linking experiment using a water-soluble carbodiimide. Depactin binds to a DNase I-Sepharose column via actin and is selectively eluted with 0.6 M KCl or 0.6 M Kl. The association constant between actin and depactin is estimated, using the column, to be 2-3 X 10(6) M-1. The content of depactin in the high-speed supernatant of the oocyte extract is determined to be 1%; this can act upon approximately 63% of the actin in the supernatant.
D051339:Actin Depolymerizing Factors; D000199:Actins / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002499:Centrifugation, Density Gradient; D003285:Contractile Proteins; D003850:Deoxyribonuclease I; D051345:Destrin; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors; D005260:Female; D008840:Microfilament Proteins; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis*; D051304:Profilins; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013215:Starfish; D014783:Viscosity
D051339:Actin Depolymerizing Factors; D000199:Actins; D000596:Amino Acids; D003285:Contractile Proteins; D051345:Destrin; D008840:Microfilament Proteins; D051304:Profilins; D011506:Proteins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I