Enzymatic removal of formalin-fixed tissues from polyester arteries, and its effect on physical properties of fibers.
A method is presented that can be utilized for the removal of formalin-fixed tissues from arterial grafts without affecting fiber material properties. Such a method could prove highly useful for studying the changes in the structure and properties of graft fiber as functions of important clinical variables. Information on what actually happens to the arterial graft material in situ with passage of time is greatly lacking in the current graft literature. The method proposed involves treatment of the formalin-fixed fiber-tissue complex with an enzymatic preparation containing enzymes derived from the porcine pancreas (pancreatin) in solution with tris(hydroxymethyl) aminomethane buffer at pH 7.6. Studies with two different grafts--a Microknit Dacron Bifurcation from a clinical patient 10 1/4 years after implantation and stored in formalin for 11 months, and a Sauvage Filamentous Velour of Dacron from an animal 24 h after implantation and stored in formalin for nine months--showed that the treatment was highly effective in completely removing the tissues at concentrations of pancreatin as low as 2.5%. The same treatment given to the virgin yarns of grafts revealed no significant effects on tensile, dimensional, and morphological properties.
D000818:Animals; D001158:Arteries / Q000378:metabolism; D001807:Blood Vessel Prosthesis; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010194:Pancreatin / Q000494:pharmacology; D011093:Polyethylene Terephthalates / Q000032:analysis*; D012972:Sodium Hydroxide; D012997:Solvents; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors
D005404:Fixatives; D011093:Polyethylene Terephthalates; D012997:Solvents; D005557:Formaldehyde; D012972:Sodium Hydroxide; D010194:Pancreatin