[Analysis of methods for optimizing lysozyme determination].
In order to optimize the detection of lysozyme in the clinic, studies on photometric and agar diffusion methods were performed with different preparations of micrococcus luteus (lysodeikticus). Living or living lyophilized preparations of micrococcus were suitable. The influence of temperature, test period and lysozyme concentration on the result was demonstrated. The lysoplate technique has several advantages in clinical routine tests, however an exact adjustment of the reagents and the test is necessary (living micrococcus, 3 hours incubation, 50 degrees C test temperature). The lysozyme measurements of different papers are transferable not without reserve and require a careful interpretation in respect of reproducibility and clinical relevance.
D000818:Animals; D005612:Freeze Drying; D006801:Humans; D005779:Immunodiffusion; D008837:Micrococcus / Q000187:drug effects; D009113:Muramidase / Q000032:analysis* / Q000494:pharmacology; D009391:Nephelometry and Turbidimetry; D010783:Photometry; D012756:Sheep
D009113:Muramidase
Methodische Untersuchungen zur Optimierung der Lysozymbestimmung.