HMG 14/17 binding affinities and DNAase I sensitivities of nucleoprotein particles.
We show that ordinary (bulk) chicken erythrocyte nucleosomes are digested more rapidly by DNAase I when they are associated with high mobility group (HMG) proteins 14/17. Digestion of HMG 14/17-nucleosome complexes, under conditions where the DNA in control nucleosomes is digested to 10 to 20% acid solubility, results in a particular depletion of single-strand DNA fragments greater than 80 nucleotides in length, relative to the DNA fragments produced from control nucleosomes. Additionally, we show that staphylococcal nuclease digests of H1/H5-depleted chromatin contain an abundant subclass of nucleosomes that are not present in appreciable amounts in digests of native chromatin. These nucleosomes contain longer lengths of DNA and have lower electrophoretic mobilities than core particles. HMG 14/17 associates highly preferentially with these nucleosomes and renders them sensitive to DNAase I, similar to what has been found for active nucleosomes.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000097:blood*; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D006609:High Mobility Group Proteins; D007700:Kinetics; D009698:Nucleoproteins / Q000097:blood*; D009707:Nucleosomes / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004277:DNA, Single-Stranded; D006609:High Mobility Group Proteins; D009698:Nucleoproteins; D009707:Nucleosomes; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I