[Autologous and allogeneic mixed lymphocyte-epidermal cell cultures in man. In vitro approach to lymphoepidermal interactions].
The ability of human normal skin epidermal cells (EC) to induce the proliferation of allogeneic and autologous lymphocytes was investigated in vitro in mixed skin cell-lymphocyte culture reaction (MSLR) as an in vitro approach of the lympho-epidermal interactions that are suspected to be involved in the pathogenesis of cutaneous inflammatory skin diseases and T cell lymphomas in man. Normal human EC were able to stimulate either allogeneic or autologous lymphocytes in MSLR in culture conditions similar to that previously reported in animals. The stimulation was higher in allogeneic than in autologous MSLR. MSLR did not appear to be affected by the trypsin treatment used to obtain EC in suspensions. Lymphocytes proliferation was maximal with a lymphocytes/EC ratio of 1:1. This suggests that autologous and allogeneic MSLR in man depends upon in vitro conditions similar to that studied in animals, and that MSLR may provide an useful tool for the study and the understanding of lympho-epidermal interactions in man.
D000328:Adult; D000818:Animals; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D012867:Skin / Q000276:immunology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D013936:Thymidine
Cultures mixtes lymphocytes-cellules épidermiques (CMLE) autologues et allogéniques chez l'homme. Approche in vitro des interactions lympho-épidermiques.