Sarcoplasmic reticulum vesicles from trembler mice: a comparison with material obtained from normal animals.
Sarcoplasmic reticulum (SR) vesicles were purified from trembler and normal mice. Comparison of their chemical composition showed that the only differences lay in their phospholipid content, the most obvious modification being the presence of lysophosphatidylcholine in preparations obtained from trembler animals. A significant increase of the Ca2+-stimulated ATPase activity promoted a drastic loss in the efficiency of the calcium pumping system of the membrane purified from trembler mice. It is suggested that the modification of the phospholipid content might explain this effect.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D051379:Mice; D008818:Mice, Neurologic Mutants; D009124:Muscle Proteins / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
D009124:Muscle Proteins; D010743:Phospholipids; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D002118:Calcium