In vitro production of granular T cells and mast cells by use of different conditioned media. Ultrastructural and functional analysis.
Normal mouse spleen cells were cultured in different conditioned media (CM). Mixed lymphocyte culture supernatant (MLC SN) was shown to promote the proliferation of cytotoxic, Thy-1+, Lyt-1+, Lyt-2+, asialo-GM-1+, weakly adherent cells with numerous vacuoles and lysosome-like cytoplasmic granules. In contrast, the Con A SN induced the proliferation of non-cytotoxic, Thy-1-, Lyt-1-, Lyt-2-, asialo-GM-1-, non-adherent cells with numerous cytoplasmic granules. The ultrastructural morphology of these cells and the cytochemical characteristics of their granules enable us to identify them as mast cells. The different effects of both CM could be related to their T-cell growth factor (TCGF) content. When the amount of TCGF of these two CM was determined (by assaying growth-stimulating activity for T-cell colonies), it appeared that the MLC SN contained larger amounts of TCGF than the Con A SN used in these experiments.
D000818:Animals; D002454:Cell Differentiation; D003208:Concanavalin A / Q000032:analysis / Q000494:pharmacology / Q000502:physiology; D003470:Culture Media; D003594:Cytoplasmic Granules / Q000032:analysis / Q000201:enzymology / Q000648:ultrastructure; D003602:Cytotoxicity, Immunologic; D005260:Female; D007376:Interleukin-2 / Q000032:analysis / Q000502:physiology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000648:ultrastructure
D003470:Culture Media; D007376:Interleukin-2; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A