[Reconstruction of the properties of smooth-muscle cell sarcolemma on planar lipid membranes].
A method is described for reconstruction of certain sarcolemma characteristics of smooth muscle cells in the small intestine of a rabbit on the planar lipid membranes (PLM). The method is based on the use of fusogenic properties of certain lipid preparations. The ultrasound dispergates of azolectin and egg lecithin in combined incubation with sarcolemma vesicles of smooth-muscle cells promote a 1.4-1.8-fold increase of the total ATPase activity of the sarcolemma. Cholesterol, dipalmithoil lecithin, total brain phospholipids, inhibit the ATPase activity. Sarcolemma vesicles preincubated with azolectin lyposomes in the ratio which induces maximum ATPase activation (sarcolemma protein: azolectin-1:6) interact intensively with PLM from azolectin. PLM modified in such a way is channel-conductive, sensitive to tetraethylammonium and sign of the applied voltage.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation; D007421:Intestine, Small / Q000502:physiology; D008081:Liposomes; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology*; D011817:Rabbits; D012508:Sarcolemma / Q000187:drug effects / Q000502:physiology*
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; C024990:asolectin; D000251:Adenosine Triphosphatases
Rekonstruktsiia svoĭstv sarkolemmy gladkomyshechnykh kletok na ploskikh lipidnykh membranakh.