Activation of rat heart phosphofructokinase-2 by insulin in vivo.
Fifteen minutes after the intravenous injection of overnight starved, anaesthetized rats with insulin, the concentration of fructose 2,6-biphosphate was increased more than 2-fold in the hearts of these animals. Insulin injection also caused a 2-3 fold increase in the Vmax of phosphofructokinase-2 with no detectable change in Km values. The effect persisted after precipitation of the enzyme with polyethylene glycol or after gel filtration through Sephadex G-25.
D000818:Animals; D004789:Enzyme Activation; D005635:Fructosediphosphates / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D005635:Fructosediphosphates; D007328:Insulin; D007527:Isoenzymes; D011092:Polyethylene Glycols; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1