[The inhibition of the electrophoretic-mobility-test: an in vitro assay for the estimation of activities in anti-lymphokine and anti-lymphocyte sera (author's transl)].
Human purified lymphocytes from peripheral blood were stimulated by Con A and the concentrated supernatants were used to induce antibodies in rabbits. These anti-lymphokine immune sera were able to inhibit the electrophoretic-mobility-test (EMT) which was performed using supernatants of lymphocytes stimulated by Con A and tanned sheep red blood cells as indicator particles. Since the inhibition is strongly dose dependent the test system appears to demonstrate and compare anti-lymphokine activities in different sera. When anti-human lymphocyte globulin (Dessau, DDR) was tested in the same test system these sera had the capacity to inhibit the EMT completely.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D004586:Electrophoresis; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D011817:Rabbits
D000961:Antilymphocyte Serum; D008222:Lymphokines
Inhibition des Elektrophorese-Mobilitäts-Tests: Eine In-vitro-Methode zur Bestimmung von Aktivitäten in Anti-Lymphokin- und Anti-Lymphozyten-Seren.