Analysis of Fc gamma receptors on human peripheral blood leukocytes by flow microfluorometry. I. Receptor distributions on monocytes, T gamma cells and cells labeled with the 3Al anti-T cell monoclonal antibody.
A dual parameter flow microfluorometric technique for accurately measuring Fc gamma receptor (FcR) expression on defined subsets of cells within a heterogeneous cell sample was developed. The FcR distribution of human peripheral blood mononuclear cells consists of three distinct peaks. By analyzing cells fluorescently labeled with the 3Al, an anti-T cell hybridoma antibody (using a green-emitting fluorophore) and for FcR (with a red-emitting fluorophore), and by using cell isolation procedures, it was shown that the cells lying within the peak with intermediate FcR density are mainly monocytes, while cells lying within the peaks with highest and lowest (i.e. negative) FcR densities are predominantly T cells. The FcR+ T cells (T gamma cells) express higher levels of the 3Al antigen than other T cells, thus demonstrating the utility of the 3Al hybridoma antibody as a marker for T gamma cells.
D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005434:Flow Cytometry; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D007962:Leukocytes / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D011961:Receptors, Fc / Q000032:analysis*; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000032:analysis*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007519:Isoantigens; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic