Sensitivity of preincubated lymphocytes to suppressor regulation.
DNA synthesis of human peripheral blood lymphocytes increases if Con A is added to the culture after 24 h preincubation at 37 degrees C. During preincubation the mitogen reactive lymphocytes lose their sensitivity to the suppressive effect of autologous mitomycin-treated mononuclear leukocytes, and of supernatants of autologous preincubated cells. The reactive lymphocytes preincubated at 4 degrees C retain their sensitivity to the suppressive effect of regulatory cells and their supernatants. It is assumed that the enhancement of mitogen response after preincubation at 37 degrees C is caused by a decrease of suppressor regulation of human lymphocytes. Prostaglandins may be regarded as one of the mediators of the suppression.
D000527:Alprostadil; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D015232:Dinoprostone; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
D008937:Mitomycins; D011458:Prostaglandins E; D003208:Concanavalin A; D016685:Mitomycin; D004247:DNA; D000527:Alprostadil; D015232:Dinoprostone