Reversed-phase paired-ion high-performance liquid chromatographic method for the separation and quantification of multiple bleomycin congeners.
A rapid, linear gradient chromatographic technique for separating and quantifying copper(II)-chelated bleomycin congeners is described. This method is also capable of separating divalent from trivalent metal chelates; determining the purity of many chemically modified bleomycins; and assaying bleomycin hydrolase activity on complex mixtures. Quantification at 280 nm is sensitive to 50 pmol and is linear at least up to 10 nmol per injection.
D000818:Animals; D001761:Bleomycin / Q000032:analysis*; D002614:Chelating Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003300:Copper / Q000032:analysis; D003546:Cysteine Endopeptidases; D006026:Glycoside Hydrolases / Q000032:analysis; D008099:Liver / Q000201:enzymology; D051379:Mice; D010084:Oxidation-Reduction
D002614:Chelating Agents; D001761:Bleomycin; D003300:Copper; D006026:Glycoside Hydrolases; D003546:Cysteine Endopeptidases; C031262:bleomycin hydrolase