Studies and observations on the use of isoelectric focusing in ultra-thin polyacrylamide gels as a method of typing human red cell phosphoglucomutase.
The technique of isoelectric focusing in ultra-thin polyacrylamide gels as a method of typing human red cell phosphoglucomutase (PGM1) has been studied. Typing was possible without the samples attaining true equilibrium focusing conditions. The isozyme patterns so obtained were clearly defined and free from distortion. The importance of assessing relative band intensities when interpreting the isozyme patterns is discussed. Our experience of using the technique to analyse casework material is described.
D000180:Acrylic Resins; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D005782:Gels; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000097:blood / Q000235:genetics; D010733:Phosphoglucomutase / Q000097:blood* / Q000235:genetics
D000180:Acrylic Resins; D005782:Gels; D007527:Isoenzymes; C016680:polyacrylamide gels; D010733:Phosphoglucomutase