Influence of hormone concentration and time factor on TSH-FSH imprinting and overlap in CHO cells.
Exposure of Chinese hamster ovarian cell cultures (cell line CHO) to TSH of FSH gave rise to hormonal imprinting. In earlier studies re-exposure after 48 h displayed a considerable increase in hormone binding. In the present experiments similar increase was demonstrated with an interval of five days. After 14 days, the increment was of lesser degree or even a decrease was noted in hormone-binding capacity. Although the CHO line originates from the target cells of gonadotropin, long-term positive imprinting was greater for TSH than for FSH, imprinting for FSH being negative rather than positive. The experimental results suggest that even very low concentrations (10(-13) mol) of hormone induce imprinting after an exposure as short as 60 min.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D005640:Follicle Stimulating Hormone; D013972:Thyrotropin