Detection of cALLA positive cells in the human normal peripheral blood by microimmunofluorometry and their fate during cultivation in diffusion chambers.
A very sensitive measuring device for immunoflourescence and a new evaluation method were applied to detect the common acute lymphoblastic leukaemia antigen (cALLA) on individual cells from normal peripheral blood. Using a monoclonal and a polyclonal antiserum a small % (about 10%) of cells was shown to carry this antigen. In double-labelling experiments these cALLA positive cells were shown not to carry surface immunoglobulin, and very little T-antigen if any. During a 13-d cultivation of normal mononuclear blood cells in diffusion chambers, the cALLA positive cells disappeared but could be detected again on day 13 at a frequency comparable to that before culture. Proliferation is discussed as the mechanism accounting for the reappearance of the cALLA positive cells, perhaps due to the influence of the T-helper cells generated during culture.
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000032:analysis; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D015260:Neprilysin; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D015260:Neprilysin