Antigen-induced suppression in man: non-specific suppression mediated via OKT8+ cells.
We have developed a simple assay for human peripheral-blood suppressor cells induced by soluble protein antigens. Suppressor cells were obtained from 6-day cultures in the presence of tetanus toxoid or PPD and were assayed in co-cultures with fresh autologous PBLs. The proliferative response in this second culture showed significant suppression varying from 50% to 90%. The suppressor cells are OKT8-positive, resistant to X irradiation and non-specific in their function. They do not impede either the function or production of TCGF in the secondary culture but we are unable to detect a non-specific suppressor factor made by these cells.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000276:immunology; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007376:Interleukin-2; D013745:Tetanus Toxoid; D014373:Tuberculin