An oligomycin-resistant Mg2+-dependent ATPase in rabbit bone marrow mitochondria.
Rabbit bone marrow mitochondria isolated by differential centrifugation showed typical oxypolarographic tracings with glutamate oxidation with ADP:O ratio of 2.9. Similar results were obtained with liver mitochondria of the same animal. When marrow mitochondria were oxydizing a substrate such as glutamate, added MgCl2 markedly stimulated state-4 respiration giving a respiratory rate identical to that of state-3. In contrast, no Mg2+-stimulation was observed with liver mitochondria. Oligomycin completely blocked the stimulation by Mg2+ but further addition of 2,4-dinitrophenol reactivated the oxygen consumption by uncoupling. Further purification of marrow mitochondria by density gradient centrifugation in Percoll provided identical oxypolarographic results. Moreover, when marrow mitochondria were incubated without Mg2+, they showed a low ATPase activity that was stimulated by 2,4-dinitrophenol and blocked by oligomycin. The presence of Mg2+ in the incubation medium uncovered an additional ATPase activity which was resistant to oligomycin and apparently unaffected by 2,4-dinitrophenol. It is concluded that bone marrow mitochondria possess two types of ATPase activity distinguished on the basis of their reactivity with oligomycin, 2,4-dinitrophenol and Mg2+.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D002642:Chick Embryo; D004140:Dinitrophenols / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011817:Rabbits
D004140:Dinitrophenols; D009840:Oligomycins; D000251:Adenosine Triphosphatases; D008274:Magnesium