Simple adaptation of a method measuring the binding of radiolabelled ligands to receptors.
This paper describes a method for monitoring the binding of radiolabelled ligands to their receptors which avoids the necessity of washing cell or membrane pellets to remove the unbound radioactivity. It is an adaptation which allows the use of a gamma counter fitted with a well crystal. By the use of a shield to screen from the gamma-counter the free ligand above the pellet, it allows the amount of ligand bound to be assessed by calculation.
D000818:Animals; D007413:Intestinal Mucosa / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D011869:Radioligand Assay / Q000295:instrumentation*; D051381:Rats; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000032:analysis
D017452:Receptors, IgG; D011971:Receptors, Immunologic