Inhibition by heparin-modulated antithrombin III of amidolysis catalysed by m beta-acrosin.
Purified m beta-acrosin catalysed amidolysis of several p-nitroanilides with C-terminal arginine residues. Antithrombin III inhibited amidolysis catalysed by the enzyme. This effect of antithrombin III was potentiated by heparin, and to a modest extent by heparan sulphate, cellulose sulphate, dextran sulphate and xylan sulphate. De-N-sulphated heparin, de-N-sulphated N-acetylated heparin, heparin of low relative molecular mass, chondroitin 4-sulphate, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid were ineffective.
D000176:Acrosin / Q000037:antagonists & inhibitors*; D000990:Antithrombin III / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D011480:Protease Inhibitors
D006025:Glycosaminoglycans; D011480:Protease Inhibitors; D000990:Antithrombin III; D006493:Heparin; D000176:Acrosin