The alternative complement pathway control protein H binds to immune complexes and serves their detection.
During solubilization of immune complexes C3b becomes fixed to the immunoglobulin part and serves as a receptor for the alternative complement pathway control protein H. The H-C3b immune complex interaction can be made detectable using 4% polyethyleneglycol to separate free from bound 125I-H. Tetanus toxoid (Te)/anti-Te complexes kept soluble with fresh serum and containing 125 IU of specific antibody bound 18% of 125I-H; when fresh serum was chelated with 10 mM EDTA, 125I-H binding was only 5%. On sucrose density gradients, the H-binding material sedimented in the range of 12 to 30 S. In 36 serum samples from rheumatoid arthritis (RA) patients and in 12 serum samples from patients with systemic lupus erythematosus (SLE), 125I-H binding was significantly elevated to 9.5 +/- 4.7% (mean +/- 1 SD) and 13.3 +/- 5.6%, respectively, while 125I-H binding by 36 normal human sera was 4 +/- 2%. RA samples (17/36, 47%) and SLE samples (9/12, 75%) had H-binding values increased by more than 2 SD above the normal mean. The serum samples were also assessed for conglutinin- and C1q-binding activities; a significant correlation between H and C1q binding was observed (P less than 0.001); there was no correlation between H and conglutinin binding. Although binding to immune complexes through its interaction with C3b, H clearly detects a population of complexes other than conglutinin, thus expanding the possibilities of further characterizing pathological complexes.
D000936:Antigen-Antibody Complex / Q000032:analysis / Q000276:immunology*; D003167:Complement Activation; D003180:Complement C3b Inactivator Proteins / Q000276:immunology*; D017242:Complement Factor H; D003170:Complement Pathway, Alternative; D006801:Humans; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols / Q000276:immunology; D013743:Tetanus Antitoxin / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology
D000936:Antigen-Antibody Complex; C493495:CFH protein, human; D003180:Complement C3b Inactivator Proteins; D007457:Iodine Radioisotopes; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid; D011092:Polyethylene Glycols; D017242:Complement Factor H