Determination of cefoperazone concentration in serum and muscle tissue with a versatile high-pressure liquid chromatographic method.
A rapid, specific, and reproducible high-pressure liquid chromatographic method was developed for the determination of cefoperazone concentration in serum and tissue. The assay uses a simple methanol extraction, with cefoxitin as the internal standard. The limits of detection are 1 to 150 micrograms/ml; the maximum coefficient of variation is 7.4%. Using the same chromatography column, muBondapak phenyl, and mobile-phase 0.005 M tetrabutylammonium buffer-acetonitrile (80:20), the method can be easily adapted for the analysis of cefoxitin and moxalactam.
D002438:Cefoperazone; D002440:Cefoxitin / Q000032:analysis; D002511:Cephalosporins / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009132:Muscles / Q000032:analysis*
D002511:Cephalosporins; D002440:Cefoxitin; D002438:Cefoperazone