[The PWM model of immunoglobulin synthesis capability of human peripheral blood lymphocytes. I. Fluorescence optical detection of cytoplasmic immunoglobulins].
We present an uncomplicated method for valuing the immunoglobulin synthesis of human peripheral blood lymphocytes using the PWM model. The detection of cytoplasmic immunoglobulins was performed by immunofluorescence. We found the following values at normal persons (n = 36): 23,3 +/- 2,8% [cIg]+-cells after PWM stimulation, 5,2 +/- 2,4% after cultivation for 7 days without PWM stimulation and less than 1% without cultivation and mitogenic challenge. Possibilities of application were discussed.
D002478:Cells, Cultured; D003593:Cytoplasm / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology*
D007136:Immunoglobulins; D011043:Pokeweed Mitogens
Das PWM-Modell des Immunglobulin-Synthese-Vermögens menschlicher peripherer Blutlymphozyten. I. Fluoreszenzoptischer Nachweis zytoplasmatischer Immunglobuline.