Suppression of the mixed lymphocyte reaction by alloantigen-primed and -stimulated human spleen lymphocytes.
This communication examines the ability of T cell-enriched preparations from human spleen to suppress lymphocyte proliferation following activation in the mixed lymphocyte reaction (MLR). To enable the generation of a subsequent responding MLR between identical partners to those employed in the initial (stimulating) MLR, spleen T cells were frozen at the time of the first culture. Spleen T cells, stimulated in this way, showed a marked ability to suppress human MLR responses, but HLA-D identity was required for the full expression of suppressor activity. The characteristics of the spleen MLR-induced suppressor T cell resemble closely those described for MLR-generated suppressor T cells in human peripheral blood.
D000328:Adult; D000368:Aged; D005260:Female; D005615:Freezing; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D013154:Spleen / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000949:Histocompatibility Antigens Class II