title: Evaluation of urea-acid system as medium of extraction for the B-group vitamins. Part II. Simplified semi-automated chemical analysis for niacin and niacinamide in cereal products.
abstract: Efficiency of a urea-acid system was evaluated as a medium of extraction for niacin and niacinamide from cereals and other selected food products. Samples were treated with 1N HCl in the presence of urea and autoclaved 30 min at 15 psi. Extracts were diluted to a known volume, filtered, and analyzed. The analytical results obtained for niacin were compared with values obtained by AOAC method 43.044. In general, the urea-acid extraction system was simple and straightforward with an efficiency comparable to the AOAC method. The analytical manifold designed uses fewer modules, gives net absorbance readings from sample and blank, and is capable of analyzing 50 samples per hour.
mesh_terms: D001322:Autoanalysis; D003124:Colorimetry; D002523:Edible Grain / Q000032:analysis*; D006851:Hydrochloric Acid; D009525:Niacin / Q000032:analysis*; D009536:Niacinamide / Q000032:analysis*; D014508:Urea
chemical_list: D009536:Niacinamide; D009525:Niacin; D014508:Urea; D006851:Hydrochloric Acid