title: [Inactivation and mutagenesis of lambda phage by O-methylhydroxylamine and O-delta-aminooxybutylhydroxylamine].
abstract: The inactivation and the mutagenesis of lambda phage Cl 857 virR by O-methylhydroxylamine (OMHA) and O-delta-aminooxybuthylhydroxylamine (delta-HA) were studied. The inactivation of OMHA-treated phage was shown to be stronger in E. coli polA cells defective in DNA-polymerase I as compared to wild-type host E. coli W3350. In contrast delta-HA caused similar phage inactivation in these two strains. Wave-type kinetics of the inactivation and the mutagenesis of phage by OMHA and delta-HA was observed. delta-HA appeared to be a more effective mutagen than OMHA: it induced higher mutant yield at a given level of inactivation.
mesh_terms: D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006898:Hydroxylamines / Q000494:pharmacology*; D007700:Kinetics; D009154:Mutation; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects
chemical_list: D006898:Hydroxylamines; C035006:O-delta-aminooxybutylhydroxylamine; C005214:methoxyamine
vernacular_title: Inaktivatsiia i mutagenez faga lambda pod deĭstviem O-metilgidroksilamina i O-del'ta-aminooksibutilgidroksilamina.