Gel filtration in 6 M guanidine hydrochloride of the alpha-subunit (and its fragments) of the receptor for immunoglobulin E.
The mol. wts of the alpha-chain of the receptor for immunoglobulin E and several of its enzyme-cleaved fragments have been evaluated by gel filtration on Sepharose 6B in 6 M guanidine HCl. The mol. wt of alpha-chains treated with endoglycosidase was 30% less than that of untreated alpha-chains. alpha-Chains digested with papain eluted in a single peak with a mol. wt approximately one-half of that of undigested alpha-chains. The results support the proposal that papain cleaves alpha-chains into two fragments of similar size [Goetze et al. (1981) Biochemistry 20, 6341-6349].
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D019791:Guanidine; D006146:Guanidines; D006596:Hexosaminidases; D007073:Immunoglobulin E; D008970:Molecular Weight; D010206:Papain; D010446:Peptide Fragments / Q000302:isolation & purification*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000302:isolation & purification*; D048809:alpha-N-Acetylgalactosaminidase
D006146:Guanidines; D010446:Peptide Fragments; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D007073:Immunoglobulin E; D006596:Hexosaminidases; D048809:alpha-N-Acetylgalactosaminidase; D010206:Papain; D019791:Guanidine