Cytological events in allo-stimulated lymphocytes triggered by exposure to alloantigens. V. Response to disrupted target cells.
H-2b lymphocytes sensitized against H-2d alloantigens or H-2k alloantigens were triggered by incubation with disrupted or intact H-2d target cells. The triggering of the lymphoid cells was stopped by fixation with glutaraldehyde. Changes in cell size, in numerical density of the endoplasmic reticulum (ER), and the Golgi apparatus (GA) as well as in the areal density of the lysosomes and the mitochondria in the lymphoblasts during the first three hours of triggering were measured with a Hewlett Packard digitizer. No significant changes in cell size or in the density of GA, lysosomes and mitochondria were observed after triggering with disrupted target cells, but a significant increase in the density of ER occurred in the lymphoblasts previously stimulated against the target cell. After triggering with intact target cells, cell size of the lymphoblasts increased, as well as the density of GA, mitochondria and lysosomes while the density of ER exhibited a decrease followed by an increase. The disrupted target cells were shown to inhibit the release of 51Cr during lysis of 51Cr-labelled target cells.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D012515:Mast-Cell Sarcoma / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008928:Mitochondria / Q000648:ultrastructure
D007519:Isoantigens