Abrogation of the in vitro generation of the cytotoxic T-cell response to a murine tumor: the role of suppressor cells.
A reproducible in vitro assay for the effect of suppressor T cells on the generation of an in vitro cytotoxic response to a metastatic murine tumor is described. Suppression in this system is maximal. The model uses splenic T cells from DBA/2 mice bearing the MDAY-D2 metastatic tumor as suppressors of the in vitro generation of a cell mediated cytotoxic response by syngeneic tumor-bearer (i.e., primed) peripheral lymph node cells stimulated with mitomycin C-treated MDAY-D2 tumor cells. The effect of the cell mediated cytotoxic response is profound, specific and mediated by splenic T cells. The suppressor T cells appear to impair precursor rather than mature cytotoxic cells. A highly immunogenic variant of MDAY-D2, namely MDWI, does not generate a suppressor response. Suppressor T cells from an MDAY-D2 tumor-bearer cannot suppress the in vitro response to MDWI. This model of potent suppressor T-cell action is highly reproducible and offers an opportunity for the analysis of why some tumors generate an effective cell mediated cytotoxic response which others stimulate dramatic suppressor T-cell response.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007700:Kinetics; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D003208:Concanavalin A