Suppression of B-cell and T-cell responses by the prostaglandin-induced T-cell-derived suppressor (PITS). II. Resolution of multiple PITS beta factors.
It has been shown that T cells cultured with prostaglandin E2 are induced to release at least two peptide-containing lymphokines (PITS). Both the high-molecular-weight (35,000; PITS alpha) and the low-molecular-weight (2000 to 5000; PITS beta) factors were shown to be potent inhibitors of both T-cell- and B-cell-dependent mitogen responses. Data are reported here which show that the PITS beta factor may be reproducibly resolved into seven components by high-pressure liquid chromatography. Although each of these components is capable of suppressing the phytohemagglutinin-induced blastogenic response, not all of these factors will suppress the in vitro antibody response to sheep erythrocytes, or a mixed lymphocyte reaction. Nevertheless, the broad-range suppressive effects previously reported for PITS beta now seem to be mediated by multiple low-molecular-weight PITS factors.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011458:Prostaglandins E / Q000276:immunology; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D008222:Lymphokines; D011458:Prostaglandins E; D013491:Suppressor Factors, Immunologic