Functional characterization of human alloreactive T cell clones.
We have investigated the functional activity of alloreactive T cell clones, generated by limiting dilutions of MLC blasts. Clones displaying helper, suppressor, or cytotoxic functions (against Class I or Class II HLA antigens) were identified, using proliferative, 51Cr-release, and hemolytique-plaque induction assays. To determine whether functionally distinct clones share determinants that trigger the AMLC reaction we have tested the capacity of selected clones to cross-stimulate each other in secondary AMLC. Our data suggest that AMLC stimulatory determinants are related to common structure antigens of the T cell receptor for alloantigen, since helper clones, for example, (TLC #19) can induce memory responses to suppressor (TLC #209) as well as cytotoxic (TLC #207) clones and vice versa. The AMLC system seems well suited for studying network regulations within the T cell compartment.
D001402:B-Lymphocytes / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000949:Histocompatibility Antigens Class II