Fidelity of replication of bacteriophage phi X174 DNA in vitro and in vivo.
Seven different revertants of bacteriophage phi X174am16 (AB5276G leads to T) have been isolated and the nature of the reversions determined by sequencing their DNA. The revertants each differ from am16 by just a single base substitution. These may be distinguished with varying degrees of ease by characteristic temperature sensitivities of growth. This has facilitated the determination of the frequency at which DNA polymerase III catalyses different types of substitution mutations in copying phi X174 DNA in vitro and in vivo. During the replicative form (RF) leads to single-stranded (SS) stage of replication in vitro, four different revertants may be readily produced according to well-defined rate laws on biasing the concentrations of dNTPs. Transversion mutations are found to be formed predominantly by purine x purine mismatching, whilst transitions are formed predominantly by G x T mismatching. The substitutions via G x T and G x A mismatches are estimated to occur at similar frequencies in vivo. The two most common revertants isolated in vivo, however, are not those readily produced during the RF leads to SS stage in vitro but are those produced on purine x purine mismatching in the SS leads to RF stage. The accuracy of the DNA polymerase in vitro appears to be similar to that in this stage in vivo. However, the overall accuracy of the RF leads to SS replication in vivo is more accurate than predicted from the measurements of the accuracy in vitro.
D000596:Amino Acids / Q000032:analysis; D010584:Bacteriophage phi X 174 / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D003854:Deoxyribonucleotides / Q000235:genetics; D009154:Mutation; D013696:Temperature; D014779:Virus Replication
D000596:Amino Acids; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003854:Deoxyribonucleotides