Anti-idiotype regulation of the formation of IgE antibody to timothy grass pollen. II. In vitro induction of suppressor T cells in mini-Marbrook cultures.
A method is described for using mini-Marbrook chambers for culturing spleen cells together with anti-idiotype antibody (anti-Id) to induce the appearance of suppressor T cells (Ts). Spleen cells that have been cultured with affinity prepared anti-Id (IgG) but not those cultured with normal IgG, suppress a secondary IgE response to timothy grass pollen antigen B (AgB) when injected intravenously into AGB-primed and boosted syngeneic recipient mice. Suppressor T cells are not induced if the spleen cells cultured with anti-Id are depleted of B cells of if the cells are cultured with the F(ab)2 fragment of anti-Id: both of these results are compatible with Fc+ cells playing a role in the induction of Ts cells by anti-Id. Analysis of soluble suppressor factors in an ELISA test suggests that both TS1 and TS2 cells may be induced by anti-Id.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D011058:Pollen / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E