[The lysozyme liberation test--a method for the demonstration of stimulation of human neutrophil granulocytes and monocytes].
A method for proof of induced secretion of lysozyme as a marker of lysosomal enzymes is described. Secretory cells are neutrophil granulocytes and monocytes. The stimulation of the cells is affected by soluble inductors. We used phytohemagglutinin (PHA), Concanavalin A (Con A), purified protein derivates (PPD) and lipopolysaccharide (LPS). Myelin basic protein has a direct stabilizing action on the membrane of lysosomes and induces an increased secretory reaction of cells after irritation. That is why it is recommended to add this substance as a supplement to the culture medium of the test. The optimal incubation parameters for the lysozyme release assay are ascertained. The method is recommended to apply in the fundamental investigation, for testing of stimulating or antiphlogistic agents and in the clinical functional diagnosis.
D000293:Adolescent; D000328:Adult; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014373:Tuberculin / Q000494:pharmacology
D008070:Lipopolysaccharides; D004676:Myelin Basic Protein; D010835:Phytohemagglutinins; D014373:Tuberculin; D003208:Concanavalin A; D006493:Heparin; D007770:L-Lactate Dehydrogenase; D009113:Muramidase
Der Lysozymfreisetzungstest--eine Methode zum Nachweis der Stimulierung neutrophiler Granulozyten und Monozyten des Menschen.