Noncovalent bonding of penicillin and cefazolin to dacron.
Dacron grafts treated with the surfactant, benzalkonium chloride or TDMAC bind significant quantities of penicillin-14C or cefazolin-14C. The treated grafts showed strong antibacterial activity which indicated that bound radioactivity corresponds to the reversible adsorption of biologically active molecules. Bound penicillin-14C or cefazolin-14C slowly dissociates when the grafts are placed in a surgically prepared muscle pouch in the rat. This slow release of antibiotic produces therapeutic levels of antibiotic in the adjacent tissue. Binding can also be achieved by in situ irrigation of surfactant treated grafts with antibiotic or by injection of the antibiotic.
D000327:Adsorption; D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D001807:Blood Vessel Prosthesis; D002437:Cefazolin / Q000494:pharmacology*; D009132:Muscles / Q000187:drug effects; D010400:Penicillin G / Q000494:pharmacology*; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011854:Radiochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013211:Staphylococcus aureus / Q000187:drug effects
D001548:Benzalkonium Compounds; D011093:Polyethylene Terephthalates; D000644:Quaternary Ammonium Compounds; C009543:tridodecylmethylammonium; D002437:Cefazolin; D010400:Penicillin G