Activating proteins for ganglioside GM2 degradation by beta-hexosaminidase isoenzymes in tissue extracts from different species.
The existence of activator proteins that stimulate hydrolysis of ganglioside GM2 by beta-hexosaminidase was demonstrated in kidney extracts from four species (rat, mouse, cattle and pig). The extent to which these preparations, as well as their human counterpart, promote ganglioside GM2 catabolism by autologous and heterologous hexosaminidase isoenzymes was compared. It was found that these activators can replace each other functionally, although the animal activator proteins do not cross-react immunochemically with an antiserum against the human protein. All preparations examined catalysed the transfer of ganglioside GM2 between liposomal membranes, indicating that the animal activator proteins act by a mechanism similar to the human GM2 activator.
D000818:Animals; D002417:Cattle; D004789:Enzyme Activation; D005678:G(M2) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006023:Glycoproteins; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007122:Immunoelectrophoresis; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D050356:Lipid Metabolism; D051379:Mice; D010447:Peptide Hydrolases; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D049231:Saposins; D013045:Species Specificity; D049128:Sphingolipid Activator Proteins; D013552:Swine; D014020:Tissue Extracts / Q000378:metabolism*; D001619:beta-N-Acetylhexosaminidases
D005732:Gangliosides; D006023:Glycoproteins; D007527:Isoenzymes; C483345:PSAP protein, human; D011506:Proteins; C483344:Psap protein, mouse; C483347:Psap protein, rat; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D014020:Tissue Extracts; D005678:G(M2) Ganglioside; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases; D010447:Peptide Hydrolases