Purification of phi X174 gene C protein.
The product of gene C of bacteriophage phi X174 is required for the replication of phiX174 single-stranded DNA in Escherichia coli cells infected with phi X174. The protein has been purified to homogeneity using an in vitro complementation system. The protein exhibits a molecular weight of 5800 under denaturing conditions. The NH2-terminal amino acid sequence of the protein is (NH2)-Met-Arg-Lys, which is consistent with the sequence predicted from the nucleotide sequence of gene C. The protein has an affinity for single-stranded DNA but less for double-stranded DNA.
D010584:Bacteriophage phi X 174 / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli; D014764:Viral Proteins / Q000302:isolation & purification*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins