[Trial of purifying tumor degenerating factor of human fibroblasts].
Tumor-degenerating factor (TDF) with the specific activity of 2.9 units/mg of protein was produced and purified by several chromatographies. The specific activity was increased to 302 units/mg of protein by DEAE-Sephadex A-50 chromatography, repeated twice. Then, this preparation was purified to the specific activity of 2,040 units/mg of protein with recovery rate of 16.6% by Con A-Sepharose and CM-Sephadex C-50 chromatographies. Finally, the specific activity was increased to 9,010 units/mg of protein with the final recovery rate of 14.6% by Blue Sepharose CL-6B chromatography.
D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002845:Chromatography; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins
Essai de purification du facteur de dégénérescence tumorale des fibroblastes humains.