title: Mutagenesis resulting from depurination is an SOS process.
abstract: When bacteriophage phi X174 am3 DNA depurinated in vitro is transfected into E. coli spheroplasts prepared from bacteria previously exposed to UV light, a strong mutagenic response is observed. This mutagenic response does not occur in spheroplasts derived from pre-irradiated bacteria carrying defective recA, recF or umuC genes. These findings indicate that mutagenesis at apurinic sites is an SOS-dependent process. The mutagenic response is not dependent on the multiplicity of transfection. This suggests that mutagenesis is not mediated by recombination.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D005814:Genes, Viral; D009154:Mutation; D011687:Purines / Q000378:metabolism*; D011995:Recombination, Genetic; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D011687:Purines; D004247:DNA