title: Mobilization of vacuolar arginine in Neurospora crassa. Mechanism and role of glutamine.
abstract: Nitrogen starvation has been shown to increase the cytosolic arginine concentration and to accelerate protein turnover in mycelia of Neurospora crassa. The cytosolic arginine is derived from a metabolically inactive vacuolar pool. Redistribution of arginine between cytosolic and vacuolar compartments is the result of mobilization of this metabolite in response to nitrogen starvation. Mobilization of arginine (and purines) also occurred in response to glutamine limitation, but arginine accumulated upon proline starvation. These observations indicate that mobilization is a consequence of glutamine limitation rather than a general response to amino acid starvation (or limitation). Analysis of the amino acid pools in mycelia subjected to starvation or limitation suggests that glutamine (or a metabolite derived from glutamine) provides a signal which determines the metabolic fate of vacuolar arginine. The results are consistent with the hypothesis that vacuolar compartmentation provides a readily available store of nitrogen-rich compounds to be utilized during differentiation or under conditions of nutritional stress.
mesh_terms: D001120:Arginine / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D009940:Organoids / Q000378:metabolism*; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D005973:Glutamine; D001120:Arginine; D009584:Nitrogen