Production of a hybridoma T suppressor cell and suppressor factor.
Two hybridoma T-cell lines were prepared by fusion of burro erythrocyte (BRBC)--specific T suppressor cells with the BW 5147 thymoma line. Culture supernatants from these T cell hybrids specifically suppressed the in vitro IgG antibody response to BRBC. Clones P3E8 and P4B10 were both removed via affinity columns bearing antibodies to BRBC and antibodies to I-Jk subregion determinants. These results indicated that the hybridoma factors were I-Jk determinant bearing and anti-idiotype determinant bearing. P3E8 supernatant was major histocompatibility complex (MHC) restricted, suppressing only H-2k haplotype spleen cells. Clone P4B10 supernatant was not MHC restricted, suppressing k, s, b, and d haplotype spleen cells. The properties of these two hybridoma factors would indicate they are derived from Ts2 cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation / Q000187:drug effects; D002460:Cell Line; D004912:Erythrocytes / Q000276:immunology; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007165:Immunosuppression Therapy; D009115:Muridae; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013945:Thymoma / Q000276:immunology
D000911:Antibodies, Monoclonal