Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry.
n-Octyl beta-D-thioglucopyranoside, a new non-ionic detergent, was synthesized. Properties, and applicability to membrane proteins, of this detergent were investigated. The detergent was easily removed by dialysis. The solubilizing power of this detergent for Escherichia coli membrane proteins was similar to that of n-octyl beta-D-glucopyranoside, which has been widely used in membrane biochemistry. No inactivation of proteins was observed after the solubilization. n-Octyl beta-D-thioglucopyranoside was superior to n-octyl beta-D-glucopyranoside in that it was much more stable and could be synthesized at much lower cost.
D002462:Cell Membrane / Q000201:enzymology; D003902:Detergents; D003956:Dialysis; D005959:Glucosidases / Q000378:metabolism; D005960:Glucosides; D008565:Membrane Proteins / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D013501:Surface-Active Agents; D013863:Thioglucosides / Q000378:metabolism*; D013865:Thioglycosides / Q000378:metabolism*
D003902:Detergents; D005960:Glucosides; D008565:Membrane Proteins; D013501:Surface-Active Agents; D013863:Thioglucosides; D013865:Thioglycosides; C018619:octyl-beta-D-glucoside; C042989:n-octyl-beta-D-thioglucopyranoside; D005959:Glucosidases; D006180:Proton-Translocating ATPases