Increase of MLC sensitivity by elimination of a non-adherent responder cell subpopulation with anti-macrophage serum.
A specific rabbit anti-rat macrophage antiserum (SAM) was prepared with a cytotoxic reactivity pattern complementary to that of a specific anti-lymphocyte serum. This was used to characterise adherent and non-adherent spleen cell subpopulations in mixed lymphocyte cultures. Adherent SAM+ cells reacted as accessory cells whereas non-adherent SAM+ cells were suppressors. Selective elimination thus achieved resulted in a highly significant increase of MLC reactivity in certain strain combinations and in conversion from non-reactivity to reactivity in others.
D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D007106:Immune Sera / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology
D007106:Immune Sera; C029764:antimacrophage globulin