Role of the Ek molecule in the generation of suppressor T cells in response to LDHB.
The role of the Ek (E alpha kE beta k) molecule in the generation of suppressor T (Ts) cells specific for lactate dehydrogenase B (LDHB) was studied using different approaches. First, lymph node cells from LDHB-primed B10.A(2R) (AkEk) nonresponder mice were shown to suppress the LDHB-specific and Ak-restricted proliferative response of T cells from the congenic responder strain B10.A(4R), which does not express E molecules (AkEo). Similarly, lymph node cells from primed CBA (AkEk) mice suppressed the anti-LDHB response of Lyt-1+Lyt-2-T cells (depleted of Lyt-2-bearing Ts cells) from the same mice. Second, in vitro priming of 2R (AkEk) T cells with LDHB-pulsed 4R (AkEo) antigen-presenting cells (APC) generated T-cell proliferation but not suppression. Third, nonresponder 2R mice were turned into responders by injecting them with LDHB-pulsed 4R APC or monoclonal Ia.m7 antibody that blocks the Ek molecule. The data demonstrate that expression of Ek molecules by the APC is necessary to generate LDHB-specific Ts cells, which in turn prevent the proliferation of Lyt-1+Lyt-2- (probably helper) cells recognizing the same antigen in the context of the Ak molecule.
D000818:Animals; D000917:Antibody Formation; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology; D000961:Antilymphocyte Serum / Q000008:administration & dosage / Q000494:pharmacology; D001667:Binding, Competitive; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013997:Time Factors
D000950:Antigens, Ly; D000961:Antilymphocyte Serum; D006183:H-2 Antigens; D007770:L-Lactate Dehydrogenase