[Restoration of the primer activity of gamma-irradiated DNA by nucleases from rat liver chromatin].
gamma-Irradiation of DNA results in a several-fold decrease of its primer activity measured as one substrate synthesis catalyzed by DNA polymerase beta. However, the combined treatment of injured DNA with 3'----5' exonuclease and endonuclease I from rat liver chromatin almost normalizes primer activity of DNA. Therefore the above-mentioned nucleases are capable of excising the gamma-injured nucleotides from 3'-OH ends of DNA.
D000818:Animals; D002843:Chromatin / Q000201:enzymology*; D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000494:pharmacology*; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000494:pharmacology*; D005720:Gamma Rays; D008099:Liver / Q000201:enzymology*; D051381:Rats
D002843:Chromatin; D003037:Cobalt Radioisotopes; D004247:DNA; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; D003850:Deoxyribonuclease I
Vosstanovlenie zatravochnoĭ aktivnosti gamma-obluchennoĭ DNK nukleazami khromatina pecheni krys.