T-cell-dependent hybrid resistance against a natural killer-resistant Moloney virus-induced lymphoma (YWA): in vitro generation of cytotoxic lymphocytes.
A Moloney leukemia virus-induced lymphoma of the A.SW strain, YWA, was used to generate cytotoxic cells in vitro. Cocultivation of spleen cells from in vivo primed syngeneic and semisyngeneic mice with X-irradiated YWA tumor cells for 5 days resulted in a strong killing activity against YWA. The cytotoxicity was H-2 restricted and mediated by Thy-1.2-positive lymphocytes. F1 hybrids with variable degrees of natural resistance to the YWA tumor in vivo all generated cytotoxic cells after secondary stimulation in vitro but showed differences in optimal responder:stimulator requirements.
D000818:Animals; D002472:Cell Transformation, Viral; D003433:Crosses, Genetic; D005260:Female; D005838:Genotype; D006084:Graft Rejection; D006824:Hybridization, Genetic; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008223:Lymphoma / Q000276:immunology*; D008297:Male; D051379:Mice; D008979:Moloney murine leukemia virus; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*