Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.
We describe defective Mu phage Mu dX (Mu d1 Bx::Tn9 [lac Apr Cmr]) which is useful for insertion mutagenesis and for construction of lac operon fusions in vivo. Mu dX retains the insertion properties of Mu d1 but produces temperature-resistant lysogens and transposes at a reduced frequency. A method is described to convert existing Mu d1 insertions to Mu dX.
D010583:Bacteriophage mu / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D007763:Lac Operon; D008242:Lysogeny; D009154:Mutation; D013045:Species Specificity; D013696:Temperature; D014161:Transduction, Genetic