Enrichment of epidermal Langerhans cells: studies with a monolayer technique and flow cytometry sorting.
Langerhans cells were enriched from epidermal cell suspensions by a monolayer technique based on the association of Langerhans cells with solid matrix-bound anti-Ia antibodies or by flow cytometry sorting of fluorescein-isothiocyanate labeled anti-Ia reactive cells. The monolayer technique gave a moderate enrichment (15-37%) whereas considerably higher purity (73-87%) was obtained by flow cytometry sorting. The identity of the enriched anti-Ia reactive cells as Langerhans cells was established by histochemical techniques or electron microscopy. The monolayer-enriched Langerhans cells could function as stimulating cells in the mixed leukocyte culture reaction and as antigen-presenting cells.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D011817:Rabbits
D000949:Histocompatibility Antigens Class II