Amplification of granulopoiesis by T cell subpopulations.
Regulation of granulopoiesis by activated splenic T lymphocyte subpopulations was investigated. The addition of syngeneic T cells activated by the mitogen concanavalin A or by mixed lymphocyte response (MLR) to normal bone marrow cultures stimulated granulopoiesis, while, in contrast, resting T cells did not. Functional analyses of Fluorescence Activated Cell Sorter (FACS) purified subpopulations of MLR activated T cells showed that both subsets enhanced granulocyte colony forming cells (CFUc) differentiation. Further studies using Ly 23+ T cells stimulated in vitro to suppress the primary generation of plaque forming cells (PFC) showed that these "suppressor cells" simultaneously enhanced CFUc differentiation. These results show that T cell "helper" function in hematopoietic regulation is not restricted to the inducer subset of T cells.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology
D000950:Antigens, Ly