Interactions between human fibroblasts and HeLa cells in vitro.
Affinity toward each other was demonstrated in co-cultures between HeLa cells and fibroblasts originating from human tumor stromal or normal tissues. Both cell types in the mixed cultures (ratio 1:1, 1:2, 2:1) proliferated normally as shown by 3H-thymidine labeling index estimation for up to 48 hr of co-culture. At ratios of fibroblasts: HeLa lower than 1:10, fibroblasts were eventually eliminated after serial passaging. It was shown that 3H-nucleotides could be transferred between heterologous cells in either direction. Contact of cells was essential for this phenomenon. Transfer of the label from HeLa to fibroblasts required a longer interaction time and was evidently lower than the transfer from fibroblasts to HeLa. 3H-thymidine incorporated into the DNA of either cell type could not be transferred from one cell to another. The model provides a means for studying neoplastic X normal (or tumour stromal) cell interactions in vitro.
D001345:Autoradiography; D001943:Breast Neoplasms / Q000503:physiopathology; D002450:Cell Communication; D002460:Cell Line; D004261:DNA Replication; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007700:Kinetics; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine