Influence of hormones on N-(4-hydroxyphenyl) retinamide inhibition of 7,12-dimethylbenz[alpha]anthracene transformation of mammary cells in organ culture.
Influence of estrogen and progesterone on the inhibitory action of N-(4-hydroxyphenyl) retinamide (4-HPR) was examined during the promotional stage of 7,12-dimethylbenz[alpha]anthracene (DMBA) transformation of the epithelial cells in culture of the whole mammary organs of BALB/c mice. In medium containing insulin, prolactin, hydrocortisone, and aldosterone, 4-HPR caused 68% inhibition of transformation as determined by the presence of nodule-like alveolar structures in the glands exposed to DMBA in vitro. Addition of estrogen and progesterone to the medium reduced this pronounced inhibitory action of 4-HPR to only 15%. While the medium containing insulin, prolactin, growth hormone, estrogen and progesterone was highly conducive to DMBA transformation, 4-HPR inhibition of transformation was limited to only 21%. The antagonistic action of the ovarian steroid hormones was present also at the level of frequency of nodule-like alveolar lesions (NLAL) per gland. Although both ovarian hormones reduced the inhibitory action of 4-HPR, on mammary cell transformation, the antagonistic action of estrogen was noticeably more pronounced.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D002369:Castration; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D017313:Fenretinide; D006728:Hormones / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques; D011374:Progesterone / Q000494:pharmacology; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology
D001551:Benz(a)Anthracenes; D004967:Estrogens; D006728:Hormones; D017313:Fenretinide; D011374:Progesterone; D014212:Tretinoin; D015127:9,10-Dimethyl-1,2-benzanthracene