Heat-sensitive radiation damage in single-stranded DNA irradiated in a bacterial extract.
The effect of heat treatment (45 degrees C) on gamma-irradiated biologically active single-stranded phi X174 DNA, dissolved in a bacterial extract, was studied. The results show that under these circumstances heat-sensitive damage is found, which is absent after irradiation in pure buffer. The damage is non-lethal and probably becomes an apurinic/apyrimidinic site, which is lethal, upon the post-irradiation heat treatment. Prolonged heating converts it into a break. The amount of the initial damage depends on the conditions of irradiation.
D010584:Bacteriophage phi X 174; D003037:Cobalt Radioisotopes; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D004926:Escherichia coli; D005720:Gamma Rays; D006358:Hot Temperature; D013997:Time Factors; D014020:Tissue Extracts
D003037:Cobalt Radioisotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014020:Tissue Extracts