Chromosomal proteins of mouse teratocarcinoma cells.
We have analyzed chromosomal proteins extracted from murine teratocarcinoma-derived stem cell lines (F9 and 12-1) and from their differentiated derivatives (12-1a) because of the differential sensitivity to DNase I digestion of these two cell types. The chromosomal DNA of stem cells is more sensitive to DNase I digestion than that of differentiated cells. Stem cell core histones are more highly acetylated than their differentiated counterparts, and certain high-mobility group (HMG) proteins from stem cells (HMG 1 and HMG 2) are found in greater amounts than in the differentiated cells though others (HMG 14 and HMG 17) occur in similar amounts. We have also identified a new HMG protein (HMG 9) that is present in stem cells and is lost following differentiation.
D000107:Acetylation; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000494:pharmacology; D006609:High Mobility Group Proteins; D006657:Histones / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D006657:Histones; D003851:Deoxyribonucleases