L-usnate-urease interactions: binding sites for polymerization.
L-usnic acid inactivates urease through a process which implicates the blockade of--SH groups in parallel to the formation of inactive polymers. Both L-alanine and L-proline partially reverses the inactivation and effectively diminishes the amount of highly polymerized protein. The amino acids also prevent the linkage of L-usnic acid on the sites of low affinity for the ligand, being then related to the sites of polymerization.
D000409:Alanine / Q000494:pharmacology; D001572:Benzofurans; D007700:Kinetics; D011392:Proline / Q000494:pharmacology; D011485:Protein Binding; D014510:Urease / Q000378:metabolism*
D001572:Benzofurans; D011392:Proline; D014510:Urease; D000409:Alanine