Density distribution profiles of T cells: TM, TG and TA cells and response patterns in autologous versus allogeneic MLRs.
Discontinuous Percoll gradients have been used to obtain selected human peripheral blood T lymphocytes without having to resort to interactions with immune complexes in the fractionation of Tm, Tg and Ta cells. Here, we could show that Ta cells represent a heterogeneous population with no distinct density profile, in contrast to light (Tg) and heavy (Tm) cells. Enriched, heavy Tm cells could be shown to be excellent responders in allogeneic MLR while failing to react in autologous MLR. In contrast, T cells of light density preferentially respond in autologous compared with allogeneic MLR.
D000818:Animals; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D011961:Receptors, Fc; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000378:metabolism
D011961:Receptors, Fc