Human T cell differentiation antigens characterizing a cytotoxic/suppressor T cell subset.
Monoclonal antibodies were raised against the leukemic T cells from a patient with chronic lymphocytic leukemia. Two antibodies, termed T411 and T811, were obtained which were reactive by indirect immunofluorescence only with cells of the T cell lineage. The T411 antibody recognized a polypeptide chain of 100,000 dalton apparent molecular weight which was present on the surface of 94 +/- 4% of peripheral blood T lymphocytes, but only on 20 +/- 8% of thymus cells. The antibody T811 reacted with a surface molecule composed of 2 poly-peptide chains of 32,000 and 34,000 dalton apparent molecular weight, which was expressed only on 25 +/- 10% of blood T lymphocytes and on 90 +/- 4% of thymus cells. Functional analysis of the T811+ and T811- T cell subsets isolated by rosetting with anti-mouse Ig coated ox erythrocytes revealed that both subpopulations were able to mount a proliferative response to allo-antigens, whereas allo-antigen induced cytotoxic cells and their precursors were only found in the T811+ subset. The pokeweed mitogen induced in vitro differentiation of B lymphocytes into immunoglobulin secreting cells was dependent on the presence of the T811- subset, whereas the T811+ T cells efficiently suppressed this differentiation.
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D007945:Leukemia, Lymphoid; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D007519:Isoantigens; D011043:Pokeweed Mitogens