S gene product: identification and membrane localization of a lysis control protein.
The product of the bacteriophage S gene has been previously shown to be required for an essential step in triggering host cell lysis. By using two different protein labeling systems, maxicells and UV-irradiated infected cells, we identified the S gene product as an 8,500-molecular-weight polypeptide associated with the cell envelope. The apparent molecular weight is significantly less than the 11,500 predicted from the S gene sequence. We were unable to confirm two previous identifications of S gene products, an acidic 15,000-molecular-weight polypeptide found by two-dimensional gel electrophoresis of infected cells and a 5,500-molecular-weight polypeptide in purified phage particles.
D001433:Bacteriolysis; D010582:Bacteriophage lambda / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005814:Genes, Viral; D008970:Molecular Weight; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D014764:Viral Proteins