Enhanced chondrocytic differentiation in chick limb bud cell cultures by inhibitors of poly(ADP-ribose) synthetase.
Inhibitors of poly(ADP-ribose) synthetase, namely nicotinamide, benzamide, m-methoxybenzamide and 3-aminobenzamide, augmented chondrocytic differentiation chick embryo limb bud mesenchymal cells, in culture. These inhibitors stimulated early appearance and massive formation of cartilage nodules in micromass cultures stage 23-24 chick embryos. They also induced nodule formation in micromass and cartilage colonies at micromass plating densities from stage 18-19 embryo Benzamide, however, did not prevent differentiated chondrocytes from undergoing a pleiotypic change in cell type. These results are compatible with the putative regulatory function of poly(ADP-ribose) on cell differentiation.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology*; D009536:Niacinamide / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013997:Time Factors
D001549:Benzamides; D009536:Niacinamide; D011065:Poly(ADP-ribose) Polymerases