lambda DNA-fragmenting actions of ascorbic acid and triose reductone in the presence of Cu2+.
Lambda DNA-fragmenting actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ were studied. The mixture of AsA or TR and Cu2+ caused a marked fragmentation of lambda DNA (3.2 X 10(7) daltons) within the first 1 min of reaction. Further incubation resulted in accumulation of the most abundant species of fragmented lambda DNA having a molecular weight of 1.3 X 10(5) daltons. The mixture of AsA or TR and Cu2+ fragmented calf thymus DNA to produce the fragmented DNA of which 5'-OH terminal groups have a mixture of free OH groups and phosphodiester linkage. The mixture of AsA or TR and Cu2+ was also found to fragment lambda DNA to produce dCMP predominantly as 5'-OH terminal nucleotides.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D010582:Bacteriophage lambda / Q000378:metabolism; D001482:Base Composition; D003300:Copper / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D008314:Malonates; D008315:Malondialdehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D008970:Molecular Weight; D009711:Nucleotides / Q000032:analysis
D004279:DNA, Viral; D008314:Malonates; D009711:Nucleotides; C010778:reductone; D008315:Malondialdehyde; D003300:Copper; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid