title: Actin, myosin and actin-binding protein in lymphoid cells from human normal and leukemic cell lines.
abstract: Actin, myosin and actin-binding protein have been identified in lymphoid cell lines of Null, B or T type of normal and malignant origin. The cells were fractionated into cytosol (Fraction 1), high KC1 extract (Fraction 2) and mainly membrane (Fraction 3). The distribution of the three contractile proteins and the ATPase activities of myosin and actomyosin in B lymphoblastoid normal and B-lymphoma malignant cell lines. Actomyosin-ATPase activity in the normal B line was much higher than it was in the Null B or T malignant lines examined. These results agree with the assumption that malignant transformation is accompanied by an impairment of the action of contractile proteins that leads to a decrease in motility.
mesh_terms: D000199:Actins / Q000097:blood*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D002460:Cell Line; D003285:Contractile Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D009218:Myosins / Q000097:blood*
chemical_list: D000199:Actins; D003285:Contractile Proteins; D000251:Adenosine Triphosphatases; D009218:Myosins