title: [Demonstration of the biosynthesis of prostaglandins from arachidonic acid by rat adrenal cortex cells in culture].
abstract: The biosynthesis of prostaglandins of the E pathway was shown in new-born rat adrenocortical cells in long-term primary culture, by direct incubation of labelled arachidonic acid or by incubation with prelabelled cells. A rapid method of extraction was developed using silanised silica cartridges.
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D007700:Kinetics; D011453:Prostaglandins / Q000096:biosynthesis*; D011454:Prostaglandins A / Q000096:biosynthesis; D011456:Prostaglandins B / Q000096:biosynthesis; D011458:Prostaglandins E / Q000096:biosynthesis; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D011453:Prostaglandins; D011454:Prostaglandins A; D011456:Prostaglandins B; D011458:Prostaglandins E; D016718:Arachidonic Acid; C042026:prostaglandin B2; C100008:prostaglandin A2
vernacular_title: Mise en évidence de la biosynthèse de prostaglandines à partir d'acide arachidonique par les cellules corticosurrénaliennes de rat en culture.