title: Phagocytosis by rat peritoneal mast cells: independence of IgG Fc-mediated and C3-mediated signals.
abstract: Phagocytosis of sheep erythrocytes (E) bearing rabbit immunoglobulin and rat C3 by rat peritoneal mast cells was quantitated by using 51Cr-labeled E and was confirmed by electron microscopy. The relative importance of IgG Fc receptor interaction in C3-mediated phagocytosis was assessed. Removal of any traces of IgG antibody by absorption of IgM antibody with protein A-Sepharose and absorption of the other reagents with sheep E had no effect on the phagocytosis of C3b- and C3bi-coated cells. IgG antibody enhanced the phagocytosis of intermediates prepared with IgM, purified components and rat C3 (EaIgMClgp4hu oxy2hu3brat) with a graded dose response, but was only additive and not synergistic with C3. The independence of the C3- and Fc-mediated signals was confirmed by using chemically produced polymers of rabbit IgG to inhibit phagocytosis. These polymers, especially tetramers and higher aggregates, completely blocked ingestion of EAIgG, but not that of EAIgMC1423b or -3bi. When IgG was substituted for IgM in the C3b intermediate, the IgG polymers inhibited about 50% of the phagocytosis. Cumulatively, the data demonstrated that in the case of rat mast cells, the stimuli to phagocytosis induced by C3 and IgG are independent; either is sufficient by itself.
mesh_terms: D000818:Animals; D003176:Complement C3 / Q000378:metabolism*; D003179:Complement C3b / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008407:Mast Cells / Q000276:immunology* / Q000648:ultrastructure; D010587:Phagocytosis; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D011951:Receptors, Complement; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D012756:Sheep
chemical_list: D003176:Complement C3; D007074:Immunoglobulin G; D011951:Receptors, Complement; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D003179:Complement C3b