Mass spectral identification of the blocked N-terminal tryptic peptide of the ATPase inhibitor from beef heart mitochondria.
The presence of a formyl blocking group at the N-terminus of the ATPase inhibitor has been identified and the partial sequence of the N-terminal peptide has been determined by fast atom bombardment and field desorption coupled to mass spectrometry. Minor discrepancies in amino acid sequence of the inhibitor between the present and published data [(1981) Proc. Natl. Acad. Sci. USA 78, 7403-7407] are reported and its relationships with other inhibitors are briefly discussed.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D010450:Endopeptidases; D013058:Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013045:Species Specificity; D014357:Trypsin; D000097795:ATPase Inhibitory Protein
D010450:Endopeptidases; D010446:Peptide Fragments; D011506:Proteins; D006180:Proton-Translocating ATPases; D014357:Trypsin; D000097795:ATPase Inhibitory Protein