Chemical quality control of [99mTc]plasmin by affinityradiochromatography.
To establish a method for radiochemical quality control of [99mTc]plasmin based on the known affinity of plasmin for lysine residues, human [113Sn]plasmin and [99mTc]plasmin formed by different methods were analyzed in an affinityradiochromatographic system of lysine coupled to CNBr-activated sepharosis. From the observed immobilizations of the radioactivity when the plasmin was bound to the lysine-sepharosis, radiochemical purities of radiolabelled plasmin could be calculated. The effects of presence of Sn(II) and tartaric and gentisic acid and the remobilizations induced by 6-aminohexanoic acid were studied.
D002846:Chromatography, Affinity / Q000379:methods; D005341:Fibrinolysin; D006801:Humans; D015609:Organotechnetium Compounds; D011786:Quality Control; D013667:Technetium
D015609:Organotechnetium Compounds; C034360:technetium Tc 99m plasmin; D013667:Technetium; D005341:Fibrinolysin