title: Inhibitory effects of T cells on in vitro granulopoiesis, erythropoiesis, and immunoglobulin production in patients with aplastic anaemia.
abstract: Coculture with mononuclear cells from aplastic anaemia (AA) patients and normal bone marrow cells produced different responses among 10 patients studied. In 4 cases, CFU-E growth was inhibited while in another 2 cases CFU-C growth was inhibited. To determine the suppressive effects of mononuclear cells from 6 AA patients, mononuclear cells were separated into T cells, B cells, and monocytes. It was found that T cells from 6 AA patients exhibited suppressive effects on normal CFU-C or CFU-E growth, whereas T cells from normal subjects enhanced the colony formation of CFU-C and CFU-E. On the other hand, monocytes fom 6 AA patients and normal subjects strongly suppressed normal CFU-C and CFU-E growth, although no significant difference was observed in both groups. The immune system in 10 AA patients was evaluated, 3 AA patients (Cases No. 1, 6, 8) exhibited an increase in Tr cells, impairment of PHA responsiveness, and low level of immunoglobulin. T cells from these patients showed a significant suppressive effect on PWM-induced differentiation of allogenic normal B cells. Thus, T cells from 3 out of 10 AA patients exhibited suppressor activities on both haematopoietic cell differentiation and B cell differentiation into plasma cells in vitro.
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000097:blood* / Q000276:immunology; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D011961:Receptors, Fc; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D007136:Immunoglobulins; D011961:Receptors, Fc