title: The use of synthetic gamma-chain peptides in the localization of the binding site(s) on human IgG1 for the Fc receptors of homologous monocytes and heterologous mouse macrophages.
abstract: In an attempt to locate precisely the sites on human IgG responsible for binding to monocytes and macrophages, synthetic peptides representative of sequences of the human gamma-chain have been used as potential inhibitors of human [125I]IgG1 binding to human monocytes and mouse macrophages. One peptide, comprising the sequence of Tyr407--Arg416 of the C gamma 3 domain, showed the same maximum inhibition of [125I]IgG binding to mouse macrophages as unlabelled IgG. Two peptides derived from sequences in the C gamma 2 domain were shown to exhibit limited inhibition of Igg binding to homologous human monocytes.
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D006801:Humans; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D014405:Tuftsin / Q000494:pharmacology
chemical_list: D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D010455:Peptides; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D014405:Tuftsin