Incorporation of killer functions from murine T-cytotoxic spleen cultures in somatic hybrids.
Fusions between the BW5147 thymoma and spleen cells with specific T-cytotoxic activity led to the recovery of a remarkably high percentage of non-specific, strictly lectin-dependent killer hybrids (70 to 95% of all hybrids tested, depending upon the particular fusion experiment). After one or several recloning steps, these hybrids behaved as very stable killers, growing in regular, interleukin-2-free growth medium, with some of them maintaining their lytic characteristics for over one year. Among these lectin-dependent killers, only two exhibited target specificity, but both lost this property within the first month after fusion. These results are discussed in light of other rare reports concerning incorporation of cytotoxic T-lymphocyte-specific functions in somatic hybrids.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000528:radiation effects; D006822:Hybrid Cells / Q000276:immunology*; D007621:Karyotyping; D037102:Lectins / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013154:Spleen / Q000166:cytology*; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D037102:Lectins