Isolation, culture, and some morphohistochemical features of endothelial cells of the pulmonary microvasculature.
Taking into account the quantitative and qualitative importance of endothelial cells of the pulmonary microvasculature (40 per cent of all lung mesenchymal cells, 26 per cent of all lung cells) the authors attempted their isolation, culture in vitro and specification of some morphohistochemical characters, using the method of Habliston et al. (1979). There were used guinea pigs and rabbits and a 0.5 and 1 per cent trypsin solution in a retrograde perfusion for the detachment of endothelial cells from their rbasement membranes. The obtained cells were maintained in culture for5 7--9 days and morphologically characterized; their caveolar enzymes were evidenced (Mg-dependent ATP-ase, acid and alkaline phosphatases, a.o.).
D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D002469:Cell Separation; D046508:Culture Techniques; D004727:Endothelium / Q000166:cytology*; D006168:Guinea Pigs; D006651:Histocytochemistry; D008168:Lung / Q000098:blood supply*; D011817:Rabbits