[Solubilization of cerebral binding sites of S-adenosyl-L-homocysteine].
We describe the solubilization of S-adenosyl-L-homocysteine binding sites from rat brain membranes; Triton X100 could solubilize near 50% of the sites. The solubilized extract exhibited the same pH dependence as the membrane extract and had the same dissociation constant and the same sensibility to S-adenosyl-L-methionine and adenosine. The solubilized extract exhibited a methylase activity which accepted phosphatidylethanolamine as substrate.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003902:Detergents; D006710:Homocysteine / Q000031:analogs & derivatives*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008780:Methyltransferases / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D017830:Octoxynol; D050918:Phosphatidylethanolamine N-Methyltransferase; D011092:Polyethylene Glycols; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000378:metabolism*; D012995:Solubility
D003902:Detergents; D006710:Homocysteine; D011092:Polyethylene Glycols; D017830:Octoxynol; D012435:S-Adenosylhomocysteine; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase
Solubilisation des sites de fixation cérébraux de la S-adénosyl-L-homocystéine.