Coordinate production by a T cell hybridoma of gamma interferon and three other lymphokine activities: multiple activities of a single lymphokine?
The T cell hybridoma FS7-20, produced by the fusion of normal B10.BR T cells to the AKR thymoma BW5147, was found when stimulated with concanavalin A (Con A) to produce the lymphokines: interleukin 2 (IL 2), interferon-gamma (IFN gamma), macrophage-activating factor (MAF), Ia induction factor IaIF), and the B cell helper factor interleukin X (IL X). The clones and subclones of FS7-20 varied dramatically in their ability to produce these lymphokines, presumably because of karyotypic variations. The ability to produce IL 2 segregated independently from the ability to produce the four other lymphokine activities; however, production of the latter activities showed a strong correlation. This coordinate production of IFN gamma, MAF, IaIF, and IL X was also observed with a cloned normal cytotoxic T cell line, cr15. These results suggest either that IFN gamma, MAF, IaIF, and IL X are all manifestations of a single molecular species or that, although these activities are different structurally, their production is controlled by a common genetic mechanism. In support of the first possibility, the IFN gamma, MAF, IaIF, and IL X activity produced by FS7-20 were all found to be equally sensitive to inactivation at pH 2. These results illustrate the usefulness of using T cell hybridomas for the study of lymphokines.
D000818:Animals; D000949:Histocompatibility Antigens Class II; D006825:Hybridomas / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000096:biosynthesis; D008222:Lymphokines / Q000096:biosynthesis*; D016215:Macrophage-Activating Factors; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes; D006377:T-Lymphocytes, Helper-Inducer
D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007371:Interferon-gamma