The effect of diamide (azodicarboxylic acid-bis-dimethylamide) on sulfhydryl group content, proteins, and the location of phosphatidylethanolamine in human blood platelets.
Treatment of human blood platelets with the thiol-oxidizing agent, diamide, causes rapid oxidation of glutathione and alterations in aggregation and release reaction. Moreover, cross-linking of proteins was observed. Three high molecular weight bands of 200 - 240 X 10(3) daltons and a band of 66 X 10(3) daltons were involved in this process. After reduction with dithiotreitol the normal pattern was received. In contrast to the erythrocyte, the cross-linking of platelet proteins was not accompanied by a reorientation of phosphatidylethanolamine in the membrane. Also a considerably smaller effect of diamide on platelet protein sulfhydryl group content was measured.
D001391:Azo Compounds / Q000494:pharmacology*; D001792:Blood Platelets / Q000032:analysis / Q000187:drug effects*; D001798:Blood Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003958:Diamide / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000097:blood*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D010084:Oxidation-Reduction; D010714:Phosphatidylethanolamines / Q000097:blood*; D010980:Platelet Membrane Glycoproteins
D001391:Azo Compounds; D001798:Blood Proteins; D006023:Glycoproteins; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D010980:Platelet Membrane Glycoproteins; D003958:Diamide; D005978:Glutathione; D004229:Dithiothreitol