Purification, sequencing and synthesis of natriuretic and vasoactive rat atrial peptide.
Mammalian atria contain potent natriuretic and diuretic substances which exist in high- and low-molecular-weight forms and which appear to be associated with atrium-specific granules. The natriuretic effect of atrial extract is largely accountable for by its renal haemodynamic effects; atrial extracts also antagonize hormone- and non-hormone-induced contraction of the isolated rabbit aorta and isolated rat kidney vasculature. We have completely purified a low-molecular-weight natriuretic and vasoactive substance from rat atria and characterized it as a 24-amino acid peptide. Synthetic peptide, produced by solid-phase synthesis, mimics biological effects of crude atrial extract and purified peptide; its activity is enhanced by slow oxidation, suggesting a disulphide (Cys 4-Cys 20) configuration for the native peptide. If secreted into blood, this atrial natriuretic peptide (' auriculin B') could be a novel peptide hormone of considerable importance to renal and cardiovascular homeostasis.
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000187:drug effects; D016275:Atrial Function; D009320:Atrial Natriuretic Factor; D007668:Kidney / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology; D009318:Natriuresis / Q000187:drug effects; D045283:Natriuretic Agents; D011506:Proteins / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009124:Muscle Proteins; D045283:Natriuretic Agents; D011506:Proteins; D009320:Atrial Natriuretic Factor