Effect of papulacandin B on the cell wall and growth of Geotrichum lactis.
Addition of the antifungal antibiotic papulacandin B to an exponential culture of Geotrichum lactis inhibited incorporation of glucose into the alkali-insoluble and alkali-soluble glucan fractions of the hyphal wall, although the rate of growth was practically unaltered. Synthesis of other cell wall components (i.e. galactomannan and chitin) was not affected. Papulacandin B also induced the proliferation of branches along the hyphae which continued to branch dichotomously resulting in a 'colonial' pattern of growth. Aculeacin A, another antifungal antibiotic that inhibited beta-glucan synthesis also caused morphological alterations similar to those described for papulacandin B. Inhibition of beta-glucan synthesis and the altered growth pattern persisted for several hours after removal of the antibiotic. Recovery of beta-glucan synthesis and restoration of the normal pattern of growth occurred simultaneously. Growth of G. lactis in L-sorbose medium also led to inhibition of beta-glucan synthesis and dichotomous branching.
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002687:Chitin Synthase / Q000378:metabolism; D005848:Geotrichum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005936:Glucans / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology; D008565:Membrane Proteins; D003904:Mitosporic Fungi / Q000187:drug effects*; D010456:Peptides, Cyclic; D029702:Schizosaccharomyces pombe Proteins
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005936:Glucans; D006027:Glycosides; D008565:Membrane Proteins; D010456:Peptides, Cyclic; D029702:Schizosaccharomyces pombe Proteins; C003045:paramylon; C011634:aculeacin A; C020789:papulacandin B; D005964:Glucosyltransferases; D002687:Chitin Synthase; C020570:1,3-beta-glucan synthase; D005947:Glucose