Effect of chromatin decondensation on the intranuclear matrix.
We have studied the effect of chromatin condensation on the morphology of the residual structures isolated from rat liver nuclei. DNAse I digestion followed by high salt extraction of nuclei in the presence of Mg++ yields residual structures consisting of a dense peripheral layer surrounding an internal network, similar to those described by Berezney and Coffey [6]. These structures are stable at low ionic strength in the presence of EDTA. When nuclei swollen in EDTA are digested with DNAse II in the presence of EDTA, structures devoid of internal network are obtained even without subsequent treatment with high salt. When swollen nuclei are exposed to Mg++ a specific recondensation of chromatin takes place. The residual structures from recondensed nuclei are similar to those isolated from control nuclei in the presence of Mg++. The results suggest that the integrity and stability of the intranuclear matrix are acquired in the course of the isolation procedure and this is favoured by chromatin condensation.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000648:ultrastructure*; D003850:Deoxyribonuclease I; D004492:Edetic Acid / Q000494:pharmacology; D004706:Endodeoxyribonucleases / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051381:Rats
D002843:Chromatin; D004492:Edetic Acid; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; C019329:deoxyribonuclease II; D008274:Magnesium