[Methods of isolating immune cells--critical assessment, artifacts].
This paper describes common used methods of immune cell separation such as separation by density-gradient, cell electrophoresis, cell adherence, affinity chromatography, isolation of cell populations by antisera and complement, by cell sorting, by monolayer and lectins. The authors give a critical evaluation in respect of yield and purity in relation to the extent of the experimental procedure and regard eventual artefacts.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002448:Cell Adhesion; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D051379:Mice; D051381:Rats; D011975:Receptors, Mitogen / Q000032:analysis; D012397:Rosette Formation
D000961:Antilymphocyte Serum; D011975:Receptors, Mitogen; D003165:Complement System Proteins
Methoden zur Isolierung von Zellen des Immunsystems--kritische Einschätzung, Artefakte. Mit 1 Abbildung.