Helix pomatia induced conversion of some 3 beta-hydroxysteroids.
After incubating a dehydroepiandrosterone solution with an enzymatic preparation from Helix pomatia, the steroid recovery was markedly decreased. The transforming activity resulted specific for some 3 beta-hydroxysteroids and it appeared to lower after storing the preparation at 4 degrees C for some days. The presence of 4-androsten-3,17-dione within the incubation products suggests that the enzymatic preparation contains at least 3 beta-hydroxysteroid: NAD oxydoreductase and 3-oxosteroid-5-4-ene isomerase. The use of large amounts of freshly dissolved Helix pomatia juice solution may lead to unreliable results in steroid analyses.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003687:Dehydroepiandrosterone / Q000378:metabolism; D006372:Helix, Snails / Q000378:metabolism*; D006914:Hydroxysteroids / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D014316:Tritium
D006914:Hydroxysteroids; D014316:Tritium; D003687:Dehydroepiandrosterone