Temperature-dependent conformational changes in isolated oligomycin-sensitive ATPase.
Isolated oligomycin-sensitive ATPase undergoes a kinetic change at 20-25 degrees C with a higher activation energy and a lower Km for ATP below this temperature range. This observation has been correlated with temperature-dependent structural changes detected by circular dichroism in the UV region in the isolated enzyme. The negative ellipticities in the 208-225 nm region, which are proportional to the alpha-helix content, increase with rise in temperature to a maximum above 25 degrees C.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology; D009840:Oligomycins / Q000494:pharmacology; D011487:Protein Conformation; D013696:Temperature
D009840:Oligomycins; D000251:Adenosine Triphosphatases