A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.
A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.
D000107:Acetylation; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D006593:Hexokinase; D007553:Isotope Labeling / Q000379:methods; D010733:Phosphoglucomutase; D010761:Phosphorus Radioisotopes; D011817:Rabbits; D012441:Saccharomyces cerevisiae; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000302:isolation & purification*; D014539:Uridine Diphosphate Sugars / Q000302:isolation & purification*
D010761:Phosphorus Radioisotopes; D014539:Uridine Diphosphate Sugars; D014537:Uridine Diphosphate N-Acetylglucosamine; D006593:Hexokinase; D010733:Phosphoglucomutase