title: Effect of cholesterol on phospholipid, RNA, and protein synthesis in Mycoplasma capricolum.
abstract: The regulatory role of cholesterol on phospholipid, RNA and protein synthesis was investigated in Mycoplasma capricolum. The addition of 2 micrograms/ml of cholesterol to cells growing slowly on lanosterol (10 micrograms/ml) caused an early stimulation of unsaturated phospholipid synthesis followed by a later stimulation of RNA and protein synthesis. Epicoprostanol, a cholesterol antagonist, sequentially inhibited these three processes. Phospholipid served as the acyl donor for the acylation of membrane proteins. Membrane-bound cholesterol correlated with a decrease in the amount of [3H]amino acids and an increase in the amount of [3H]oleate found in two membrane proteins (MW 78 kDa and 26 kDa). Taken together, the results suggest that a small amount of membrane-associated cholesterol serves as a signal for membrane biogenesis and, in turn, macromolecular synthesis and cell growth.
mesh_terms: D000215:Acylation; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002784:Cholesterol / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D007700:Kinetics; D008074:Lipoproteins / Q000096:biosynthesis; D008563:Membrane Lipids / Q000096:biosynthesis; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009174:Mycoplasma / Q000187:drug effects* / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D012329:RNA, Bacterial / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D008074:Lipoproteins; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D012329:RNA, Bacterial; D002784:Cholesterol