Determination of niacin metabolites 1-methyl-5-carboxylamide-2-pyridone and N-1-methylnicotinamide in urine by high-performance liquid chromatography.
A test suitable for detecting latent niacin deficiency was developed. It measures the 24-h urinary output of the two major metabolites of niacin, 1-methyl-5-carboxylamide-2-pyridone and N-1-methylnicotinamide. The two metabolites were isolated from urine using separate ion-exchange extractions. They and their two internal standards were quantitated simultaneously by high-performance liquid chromatography using a reversed-phase ion pairing separation. Detection was by absorbance at 254 nm.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009525:Niacin / Q000378:metabolism*; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000652:urine; D012015:Reference Standards
D009536:Niacinamide; D009525:Niacin; C005211:N'-methyl-2-pyridone-5-carboxamide; C024058:N(1)-methylnicotinamide