Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cells.
Treatment of intact 3T3 cells with biologically active phorbol esters causes a progressive decline in the total activity of Ca2+-sensitive, phospholipid-dependent protein kinase (protein kinase C) measured in cell-free, detergent-solubilized, extracts. The effect is dose-dependent and reversible; protein kinase C activity reappears when the phorbol esters are removed from the medium.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002460:Cell Line; D051379:Mice; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D010743:Phospholipids / Q000502:physiology*; D011493:Protein Kinase C; D047428:Protein Kinase Inhibitors; D013997:Time Factors
D010703:Phorbol Esters; D010704:Phorbols; D010743:Phospholipids; D047428:Protein Kinase Inhibitors; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D002118:Calcium