[Accumulation of one of the histone H1 subfractions and increase in chromatin resistance to the nuclease effect in the course of carp and grass carp spermatogenesis].
Chromatin of immature testes and sperma of grass carp and carp was hydrolyzed by micrococcal nuclease and DNA I. The hydrolysis kinetics was studied. Chromatin of sperm maintains the nucleosomal repeat typical to somatic cells and is more resistant to the nuclease effect than chromatin of testes. Histones H1 and H2B of grass carp differ from the respective histones of the calf thymus. The core histones of sperm testes and liver chromatin in grass carp and carp have similar electrophoretic mobility. However the amount of slowly moving subfraction of histone H1 increases in the course of spermatogenesis. The amino acid composition of histone H1 from the carp sperm is characterized by a high lysine (34.6%) and low glycine (4.5%) content. A histone H1 molecule contains one tyrosine. The lysine/arginine ratio is 21.6 which is considerably higher than in H1-like histones of sperma in other species (for instance of Echinodermata).
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine / Q000032:analysis; D002347:Carps / Q000502:physiology*; D002843:Chromatin / Q000502:physiology*; D003530:Cyprinidae / Q000502:physiology*; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000032:analysis; D008297:Male; D008836:Micrococcal Nuclease; D013045:Species Specificity; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology
D000596:Amino Acids; D002843:Chromatin; D006657:Histones; D001120:Arginine; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D008239:Lysine
Nakoplenie odnoĭ iz subfraktsiĭ gistona H1 i uvelichenie ustoĭchivosti khromatina k deĭstviiu nukleaz v khode spermatogeneza karpa i belogo amura.