Hapten-specific help mediated by an anti-ABA T cell line.
Long-term cultures of functional T cells were grown from mice injected with azobenzene arsonate (ABA) coupled spleen cells or mice painted with the diazonium salt of p-phenylarsonate. All cell lines produced delayed hypersensitivity to ABA. When tested for helper function in an antibody-forming cell assay only one line, AA3, helped plaque-forming cell formation. It was able to help anti-FLU response of FLU-HGG primed B cells to ABA-KLH-FLU. The help required linkage of the ABA and FLU determinants because cultures stimulated with a mixture of ABA-KLH and TNP-KLH-FLU did not show increased PFC. These experiments demonstrate hapten-specific help in a system which eliminates involvement of passively absorbed antibody in hapten recognition.
D000818:Animals; D000917:Antibody Formation; D001391:Azo Compounds / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007114:Immunization; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D006241:Haptens; D010132:p-Azobenzenearsonate; D006433:Hemocyanins