Different muscle-specific forms of rabbit skeletal muscle alpha-actinin.
The structures and functions of the two alpha-actinin isoforms [R. Kobayashi et al. (1983) Eur. J. Biochem. 133, 607-611] isolated from rabbit longissimus dorsi and psoas muscles were compared. One-dimensional and two-dimensional electrophoretic analyses showed that the two alpha-actinins were different from each other in their subunit chain weights and isoelectric points. The Stokes' radius of the longissimus dorsi and psoas alpha-actinins was 7.4 nm and 7.0 nm, respectively. The amino acid analyses showed that, although the two alpha-actinins are similar in their amino acid compositions, longissimus dorsi alpha-actinin contains more aspartic acid and isoleucine than psoas alpha-actinin but fewer glycine and valine residues. Analysis of the soluble tryptic peptides by two-dimensional mapping revealed that the two alpha-actinins had major differences. These data suggested that the two isoforms are the products of at least two different genes. Despite these differences, both alpha-actinins share a number of common properties. Both alpha-actinins contain a 55-kDa peptide resistant to trypsin. The two proteins show no differences in actomyosin turbidity assays. ATPase assays and F-actin binding assays of alpha-actinin activity. Immunological examination indicates that the two alpha-actinins share antigenic determinants in common.
D000185:Actinin / Q000032:analysis* / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D009218:Myosins / Q000378:metabolism; D009391:Nephelometry and Turbidimetry; D011817:Rabbits; D014357:Trypsin / Q000378:metabolism
D000199:Actins; D000596:Amino Acids; D009124:Muscle Proteins; D000185:Actinin; D000205:Actomyosin; D014357:Trypsin; D000251:Adenosine Triphosphatases; D009218:Myosins