[Ca-ATPase self-fluorescence in the sarcoplasmic reticulum of the rabbit skeletal muscle].
The quenching of the intrinsic protein fluorescence of sarcoplasmic reticulum Ca-ATPase from the rabbit skeletal muscles by hydrophylic (NaI, CsCl) or hydrophobic (pyrene, fluorescamine) substances has been studied. CsCl (up to 1 M) has been shown not to affect the intrinsic protein fluorescence while NaI (250 mM) quenches it at 15%, pyrene (8 mkM) decreases the intrinsic fluorescence of Ca-ATPase at 35% and fluorescamine (up to 40 mkM)--at 80%. Possible mechanisms of the interaction of the quenchers with the intrinsic fluorescence of sarcoplasmic reticulum Ca-ATPase are being discussed.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000502:physiology*; D002586:Cesium / Q000494:pharmacology; D002712:Chlorides; D004305:Dose-Response Relationship, Drug; D005450:Fluorescamine / Q000494:pharmacology; D005453:Fluorescence; D011487:Protein Conformation; D011721:Pyrenes / Q000494:pharmacology; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology*; D012974:Sodium Iodide / Q000494:pharmacology
D002712:Chlorides; D011721:Pyrenes; D002586:Cesium; D005450:Fluorescamine; C030984:pyrene; D000252:Calcium-Transporting ATPases; D012974:Sodium Iodide; C028019:cesium chloride
Issledovanie sobstvennoĭ fluorestsentsii Ca-ATFazy sarkoplazmaticheskogo retikulma skeletnykh myshts krolika.