title: Separation of synaptic junctional complexes from thin layers of rabbit cerebral cortex.
abstract: Synaptic junctional fractions were separated from rabbit brain by procedures based on combining the methods of Cotman and Taylor [4], Orosz et al. [16, 17] and Lisman et al. [13]. Thin layers of cerebral cortices were homogenized to obtain a crude mitochondrial-synaptosomal fraction. The sedimentation rates of mitochondria and mitochondria containing synaptosomes were increased by raising the density of mitochondria with an insoluble dense formazan deposit inside mitochondria after iodo-nitrotetrazolium treatment. The synaptic plasma membrane fraction isolated by this method contained no mitochondrial contamination. After Triton X-100 treatment the insoluble residues of the detergent were centrifuged through discontinuous sucrose gradients. A great enrichment of morphologically identifiable intact synaptic junctions was observed in some of the obtained interface layers.
mesh_terms: D000818:Animals; D002500:Centrifugation, Isopycnic / Q000379:methods; D002540:Cerebral Cortex / Q000032:analysis* / Q000648:ultrastructure; D003902:Detergents; D008928:Mitochondria / Q000032:analysis* / Q000648:ultrastructure; D017830:Octoxynol; D011092:Polyethylene Glycols; D011817:Rabbits; D011869:Radioligand Assay; D013570:Synaptic Membranes / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D003902:Detergents; D011092:Polyethylene Glycols; D017830:Octoxynol