[Reduction of ferricytochrome P-450 with eosin photoradicals].
Photoreduction of ferricytochrome P-450 with eosine radicals was studied by flash-photolysis. Reactivity of reduced protein was estimated by CO binding. It has been stated that both, at direct reduction of cytochrome P-450 and at its recombination with CO after its photodissociation of carboxycomplex the reaction consists of three stages with similar rate constants but different weight ratio of corresponding stages. This result well agrees with the supposition about non-equilibrium (in the course of reduction) distribution of cytochrome P-450 conformers.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004801:Eosine Yellowish-(YS) / Q000378:metabolism*; D005609:Free Radicals; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D010777:Photochemistry; D051381:Rats
D005609:Free Radicals; D003577:Cytochrome P-450 Enzyme System; D004801:Eosine Yellowish-(YS)
Vosstanovlenie ferritsitokhroma P-450 fotoradikalami éozina.