Reactivation and mutagenesis of UV irradiated lambda phage in bacteria treated with platinum (II) compounds.
Treatment of wild type Escherichia coli with cis -Pt(NH3)2Cl2 increased the survival and frequency of clear plaques formation of lambda phage damaged by UV radiation. The reactivation process was present in an uvrA mutant and abolished in a lexA host. Trans-Pt(NH3)2Cl2 and [Pt(dien) Cl]Cl (dien = 2HN-CH2-CH2NH-CH2-CH2-NH2) which, inhibited DNA synthesis less than the cis isomer or not at all, respectively, induced only a slight increase in survival of UV irradiated phage while mutagenesis was not affected. A relation exists between the reactivation of UV damaged phage in bacteria treated with these three compounds and their recently reported abilities to inhibit DNA synthesis and induce recA protein.
D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D002945:Cisplatin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D009154:Mutation; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay
C032799:chlorodiethylenetriamine platinum; C034697:transplatin; D002945:Cisplatin