The adult fast isozyme of myosin is present in a nerve-muscle tissue culture system.
Organotypic nerve-muscle cultures were prepared from foetal mouse spinal cord and adult mouse muscle fibres. In this system, the adult fibres degenerate and new myotubes form. The regenerated muscle fibres become innervated, develop cross-striations, and survive for several months. We have investigated the isozymes of myosin present in these muscle fibres using histochemistry and immunocytochemistry with antibodies to rat embryonic, neonatal, and adult fast myosins. We demonstrate that some of the regenerated fibres contain adult fast but not embryonic or neonatal myosin. This is the first demonstration of the production of adult myosin heavy chain in tissue culture. This system therefore offers the possibility for further study of the development of adult myosin isoforms in vitro.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D009132:Muscles / Q000033:anatomy & histology / Q000502:physiology*; D009218:Myosins / Q000378:metabolism*; D009924:Organ Culture Techniques; D012038:Regeneration; D013116:Spinal Cord / Q000196:embryology* / Q000502:physiology
D007527:Isoenzymes; D000251:Adenosine Triphosphatases; D009218:Myosins