Setting up and running a microscope-based flow cytometer.
A noncommercial flow cytometry system was set up in a laboratory with no engineering staff attached. The system is based upon a conventional fluorescence microscope and a new flow principle reported by H. Steen and T. Lindmo (Science 204:403, 1979). A few modifications and additions have been introduced. The instrument has proven to be a high-resolution system, easy to adjust and control, giving coefficients of variation of cellular DNA down to 0.8% and of fluorescent polyethylene microspheres down to 0.9%.
D000818:Animals; D004247:DNA / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D008863:Microspheres; D051381:Rats; D012212:Rheology; D014337:Trout
D004247:DNA