A comparison of experimental and theoretical melting maps for replicative form of phi X174 DNA.
A previously elaborated technique for fixing a chosen partially melted state of DNA with glyoxal was used in a study of the melting process of the replicative form (RF III) of phi X174 DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoretical melting maps obtained in (4) and (6). This comparison clearly shows that only rigorous calculations (4) and not the ones proposed by Azbel (6,7) correctly predict the course of RF III melting.
D010584:Bacteriophage phi X 174 / Q000235:genetics*; D004261:DNA Replication; D008854:Microscopy, Electron; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D014779:Virus Replication