An apparent lack of HLA restriction in the stimulation of granulocyte-macrophage colony formation from normal human null cells by helper T lymphocytes.
Haemopoietic progenitor cells capable of producing granulocyte-macrophage (GM) colonies have been demonstrated in the 'null' lymphocyte population of normal peripheral blood. The helper and suppressor roles of different T cell subpopulations have been implicated in the regulation of granulopoiesis in disease as well as in normal individuals. However, it is not certain whether such interactions between T cells and progenitor cells are HLA-restricted. We undertook further investigation of the effect of T cell subpopulations (TG and TnonG) on GM colony formation in vitro. In particular, we studied the possibility of HLA restriction in this process. Our results demonstrate that the enhancement of GM colony growth by T lymphocytes is not restricted by HLA compatibility between T cells and null cells, that such stimulation is radio-sensitive and that it is provided by the TnonG cell subpopulation.
D000328:Adult; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000502:physiology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008215:Lymphocytes, Null / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008937:Mitomycins / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects
D006680:HLA Antigens; D008937:Mitomycins