Assessment of chemotactic activity by means of implanted sponges. With 4 figures.
In order to estimate chemotactic activity in vivo, a model of acute inflammation, the subdermal implantation of inert sponges was used. In vitro produced chemotactic active lymphokine preparations had no stimulating effect on the migration of leucocytes into the sponges. But PPD (12.5 micrograms/sponge) and PHA (40 microliters/sponge) induced in sensitized and non-sensitized mice, respectively, locally chemotactic active mediators, which increased the migration into the sponges. Phenylbutazone, dexamethasone and cyclophosphamide prevented the PPD- and PHA-induced elevated migration of leucocytes into the sponges.
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D007158:Immunologic Techniques; D007249:Inflammation / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis / Q000494:pharmacology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009504:Neutrophils; D010653:Phenylbutazone / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D014373:Tuberculin / Q000276:immunology
D008222:Lymphokines; D010835:Phytohemagglutinins; D014373:Tuberculin; D003520:Cyclophosphamide; D010653:Phenylbutazone