Nephelometric determination of plasminogen and plasmin inhibitors in human plasma using fibrin suspension as a substrate.
A nephelometric method is described for determination of plasminogen and two types of plasmin inhibitors in human plasma having different affinity toward plasmin. This method is based on the kinetic analysis of effects of whole plasma and plasmin inhibitor fraction obtained from plasma on the activity of exogenously added plasminogen which was determined by measuring the decrease of light scattering of fibrin suspension. With this method we have determined the activity of plasminogen and two types of inhibitors in the plasma of normal subjects and patients with high fibrinogen degradation product values. They include patients with various malignant tumors with DIC, chronic renal failure, sepsis, vascular diseases, and liver cirrhosis with hepatoma.
D005260:Female; D005337:Fibrin; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005341:Fibrinolysin / Q000037:antagonists & inhibitors*; D006801:Humans; D008297:Male; D009391:Nephelometry and Turbidimetry; D010958:Plasminogen / Q000032:analysis*; D000979:alpha-2-Antiplasmin / Q000032:analysis*
D005338:Fibrin Fibrinogen Degradation Products; D000979:alpha-2-Antiplasmin; D005337:Fibrin; D010958:Plasminogen; D005341:Fibrinolysin