Acyl-enzymes as thrombolytic agents in dog models of venous thrombosis and pulmonary embolism.
A quantitative model of venous thrombosis in the beagle dog is described. The model was adapted to permit ageing of isolated experimental clots in vivo. A model of acute pulmonary embolism in this species is also described. In the venous thrombosis model, infusion of streptokinase (SK) or SK-activated human plasmin gave significant lysis but bolus doses of SK. plasmin complex were ineffective. Active site anisoylated derivatives of SK. plasminogen complex, SK-activated plasmin and activator-free plasmin were all active when given as bolus doses in both models. At lytic doses, the acyl-enzymes caused fewer side-effects attributable to plasminaemia than the corresponding unmodified enzymes.
D000818:Animals; D016255:Anistreplase; D004285:Dogs; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005341:Fibrinolysin / Q000627:therapeutic use*; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D008297:Male; D010958:Plasminogen / Q000627:therapeutic use*; D011655:Pulmonary Embolism / Q000097:blood / Q000188:drug therapy*; D013300:Streptokinase / Q000627:therapeutic use*; D013924:Thrombophlebitis / Q000097:blood / Q000188:drug therapy*
D005338:Fibrin Fibrinogen Degradation Products; D005343:Fibrinolytic Agents; D016255:Anistreplase; C041947:4-anisoyl activator-free plasmin; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin