[Structural requirements of mitochondrial D-beta-hydroxybutyrate dehydrogenase with respect to its coenzyme].
The purpose of this work was to test structural analogs of NAD+ in order to know enzyme requirements of chemical structure of coenzyme to get catalytic activity and, in a other hand to see which chemical parts of the coenzyme were involved in the coenzyme binding to the active site. The binding of the coenzyme analog at the catalytic site requires an adenosine diphosphoribose structure without any additional phosphate group on the ribose linked to adenine.
D000818:Animals; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009243:NAD / Q000031:analogs & derivatives / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D009243:NAD; D006884:Hydroxybutyrate Dehydrogenase
Exigences structurales de la D-beta-hydroxybutyrate déshydrogénase de mitochondries vis-à-vis du coenzyme.