Seeding of Dacron vascular prostheses with endothelium of aortic origin.
Seeding of autologous venous endothelium on Dacron vascular prostheses in dogs results in endothelial coverage of the prosthetic flow surface 4-6 weeks after implantation. Canine aortic endothelium, in contrast, usually fails to completely cover an unseeded prosthesis by pannus ingrowth even over much longer periods. To see if the success of endothelial seeding stems from a difference in the ability of venous and aortic endothelium to grow on prosthetic surfaces, we seeded freshly harvested autologous aortic endothelium on Dacron velour infrarenal aortic prostheses in dogs. Six weeks after surgery these prostheses showed the features reported to be typical of seeded prostheses. Scanning electron micrographs showed a luminal lining of flat polygonal cells without fibrin or adherent formed blood elements, and light microscopy showed an underlying layer containing aligned spindle-shaped cells with elongated nuclei and cell-lined subluminal channels. Control prostheses were covered with fibrin and platelet-rich thrombi everywhere except for limited pannus ingrowth at anastomotic sites. The results suggest that the success of autologous endothelial seeding cannot be ascribed to inherent differences in properties such as mitotic capacity or fibrinolysis between venous and aortic endothelium. The formation of complete endothelial linings by seeding must instead result from a more favorable condition for endothelial cell growth created by the cell harvesting or seeding process itself.
D000818:Animals; D001012:Aorta, Abdominal / Q000033:anatomy & histology / Q000601:surgery*; D001807:Blood Vessel Prosthesis; D004285:Dogs; D004727:Endothelium / Q000473:pathology; D005260:Female; D008855:Microscopy, Electron, Scanning; D011093:Polyethylene Terephthalates; D013997:Time Factors
D011093:Polyethylene Terephthalates