Glutamate dehydrogenase from wild type Neurospora crassa; inactivation by photo-oxidation.
The NADP dependent glutamate dehydrogenase from wild type Neurospora crassa is inactivated by exposure to light in the presence of the dye, Methylene Blue. Photo-oxidation appears to disturb the conformational equilibrium which controls the activity of this enzyme. Data obtained suggests that the modified group is the same as that reactive to the histidine reagent, diethylpyrocarbonate.
D001665:Binding Sites; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000528:radiation effects; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D010084:Oxidation-Reduction
D005969:Glutamate Dehydrogenase; D008751:Methylene Blue