[Purification of epidermal G2-chalone using immunoaffinity chromatography].
Epidermal G2-chalone was purified from rat skin extract by immunoaffinity chromatography with the use of mono-specific antibodies coupled to CNBr-activated sepharose 4B. During SDS PAAG electrophoresis, it produced a single band with a molecular weight of 13000 dalton. G2-chalone purified in this way had a 60% antimitotic inhibitory effect on external ear epidermocytes in mice in a dose of 2 micrograms/g bw but had no effect on the mitosis in the sebaceous glands.
D000818:Animals; D050150:Chalones; D002846:Chromatography, Affinity / Q000379:methods; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009930:Organic Chemicals; D051381:Rats; D012867:Skin / Q000032:analysis*
D050150:Chalones; C025912:G(2) chalone; D006131:Growth Inhibitors; D009930:Organic Chemicals
Ochistka épidermal'nogo G2-keĭlona s pomoshch'iu immunoaffinnoĭ khromatografii.