Macrophage cell cycling: influence on Fc receptors and antibody-dependent phagocytosis.
The influence of cell cycling on the density and binding properties of IgG2a Fc receptors and their associated antibody-dependent phagocytic activity was investigated with the P388D1 murine macrophage cell line. Unseparated macrophages and subpopulations of elutriated macrophages, enriched for cells in G1, S, and G2 + M phases were compared to detect possible differences in IgG2a-dependent phagocytosis. Suspensions of G2 + M phase cells were appreciably enhanced in phagocytic activity over G1-phase cells, which were less phagocytic than unseparated macrophage populations. An analysis of the binding of 125I-IgG2a myeloma protein disclosed that the IgG2a Fc receptor avidity remained essentially unchanged during cell cycle traverse, whereas the number of IgG2a Fc receptors more than doubled as cells cycled from G1 to G2 + M (1.5 X 10(5) vs 3.4 X 10(5) receptors per cell). With their increased size relative to G1 cells, and the resultant increase in receptor number, G2 phase cells should have more productive collisions with the antibody-coated target cells and greater phagocytic capacity.
D000818:Animals; D002453:Cell Cycle; D002469:Cell Separation; D005434:Flow Cytometry; D007074:Immunoglobulin G / Q000378:metabolism; D007399:Interphase; D007518:Isoantibodies / Q000276:immunology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010587:Phagocytosis; D011961:Receptors, Fc / Q000032:analysis*; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000032:analysis
D007074:Immunoglobulin G; D007518:Isoantibodies; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic