In vitro enhancement of AA-degrading activity in human plasma with the plasminogen activator streptokinase.
Radiolabelled protein AA was coupled to cyanogen-bromide-activated Sepharose 6 MB and used as a substrate to determine the AA-degrading activity of enzymes in solution. The applicability of the substrate was tested with an elastase preparation known to have AA-degrading activity. The substrate was used to determine the AA-degrading activity in fractions of normal human serum in the presence and absence of the plasminogen activator streptokinase. The AA-degrading activity was increased in fractions in which streptokinase-induced plasminogen activation had occurred. The increase in activity could be inhibited with antibodies to plasminogen. AA-degrading activity could also be increased in whole human plasma by the addition of streptokinase.
D000682:Amyloid; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000937:Antigen-Antibody Reactions; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007700:Kinetics; D010958:Plasminogen / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D000685:Serum Amyloid A Protein; D013300:Streptokinase / Q000494:pharmacology*; D013379:Substrate Specificity
D000682:Amyloid; D000906:Antibodies; D007074:Immunoglobulin G; D000685:Serum Amyloid A Protein; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin