Two human colon tumor cell lines with similar nuclease sensitivities have different ethidium bromide binding characteristics.
Chromatin from two human colon adenocarcinoma cell lines (HT-29 and LoVo) showed similar digestion kinetics when sensitivities to DNase I and micrococcal nuclease were examined. Chromatin conformations were probed by examining the binding of ethidium bromide. A Scatchard plot revealed that both chromatins bound the same amount of ethidium bromide per mole of DNA, but the DNA from LoVo cells was more accessible to the intercalator. The results indicate that differences in chromatin conformation are not necessarily accompanied by different nuclease sensitivities.
D000230:Adenocarcinoma / Q000201:enzymology / Q000378:metabolism*; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004996:Ethidium / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation
D002843:Chromatin; D009363:Neoplasm Proteins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D004996:Ethidium