Differential ability of mitogen-stimulated human leukocyte-conditioned media to induce Fc receptors in human leukemia cells.
The ability of mitogen-stimulated human leukocyte-conditioned media (M-CM) to induce the in vitro differentiation of various human leukemic cell lines was evaluated by measuring the appearance of Fc receptors (FcR) through their ability to form EA rosettes. Only cells of myeloid lineage were induced by M-CM to express FcR; T-, and B-, and non-T/non-B cells failed to respond. As determined with ML-1, a line of human myeloblastic leukemia cells, pokeweed mitogen-conditioned medium, at concentrations of 1-10%, stimulated the expression of FcR in 70-98% of the cells within 1 day after treatment. Phytohemagglutinin-, concanavalin A-, and lipopolysaccharide-conditioned media were less active. The FcR-inducing activity was partially separated from M-CM by chromatography on Sephadex G-75. It was stable between pH 4 and 10, and lost activity at temperatures above 40 degrees C.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000032:analysis*; D003470:Culture Media; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007938:Leukemia / Q000032:analysis*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology; D007951:Leukemia, Myeloid / Q000032:analysis; D015470:Leukemia, Myeloid, Acute / Q000032:analysis; D007959:Lymphocyte Culture Test, Mixed; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*
D003470:Culture Media; C034102:FcRI protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011043:Pokeweed Mitogens; D011506:Proteins