On the structure of active chromatin. A flow linear dichroism study of chromatin fractionated by nuclease digestion.
Nuclei from Ehrlich ascites cells were treated with micrococcal nuclease or DNase I and extracted with 1 mM EDTA. The chromatin fraction released by this procedure showed positive flow linear dichroism (LD) at low salt (2 mM NaCl) while the non-released fraction had negative LD. Furthermore, the chromatin structure responsible for the positive LD was found to be labile: The LD was reduced by heat (37 degrees C) or RNase treatment and inverted to a negative LD by electric fields (10 kV/cm) and by the presence of DNA binding dyes.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D003850:Deoxyribonuclease I; D004492:Edetic Acid; D004558:Electric Stimulation; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology; D008739:Methyl Green / Q000494:pharmacology; D008836:Micrococcal Nuclease / Q000378:metabolism; D009994:Osmolar Concentration; D012260:Ribonucleases / Q000378:metabolism; D013057:Spectrum Analysis
D002843:Chromatin; D008739:Methyl Green; D004492:Edetic Acid; D004706:Endodeoxyribonucleases; D012260:Ribonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D004996:Ethidium