Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without affecting the amino acid, glutamine, encoded by the overlapping gene D. The phi X174rb(E)557 mutant does not lyse infected Escherichia coli C and therefore results in the accumulation of a large number intracellular mature phage particles. Thus, the mutation inactivates production of the gene E lytic product, presumably by blocking translation of gene E, without affecting other phage functions.
D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D012333:RNA, Messenger; D014764:Viral Proteins