The effect of PS-K, a protein bound polysaccharide, on immune responses against allogeneic antigens.
A single dose of PS-K administered to C57B1/6J mice after immunization augmented both the humoral and cellular allogeneic responses against P815 tumor cells. PS-K addition to primary alloantigen sensitization cultures (C57B1/6J spleen cells against X-irradiated P815 cells) resulted in augmented 3H-thymidine uptake and cell mediated cytolytic activity. Ten daily administrations of PS-K to DBA/2J mice after implantation of a mammary tumor of DBA/2HaDD origin results in increased tumor regression and prolonged survival of the mice. PS-K addition to human mixed lymphocyte cultures caused an augmentation of both cell mediated cytolytic activity and 3H thymidine uptake. The dose dependence of the PS-K effects were described by a bell shaped curve and PS-K did not appear to affect the day of peak development of the various responses. The effects were consistently greater if PS-K was administered at the same time or after antigen presentation. Thus, the immunoaugmenting effects induced by PS-K were similar in each model system tested.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000917:Antibody Formation / Q000187:drug effects; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011509:Proteoglycans / Q000008:administration & dosage* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D011509:Proteoglycans; C010770:polysaccharide-K