Cholesterol sulfotransferase of newborn mouse epidermis.
Recent studies of the epidermis in patients with recessive X-linked ichthyosis indicate that cholesterol sulfate is an important endogenous substrate for steroid sulfatase in the stratum corneum. We report here that cholesterol sulfotransferase, which converts cholesterol to cholesterol sulfate, is present in the lower living epidermis. Epidermal cytosol also sulfates phenols and some steroids, and such reactions may be important in defending against compounds absorbed percutaneously and in modulating the pharmacologic activity of topical medicaments. Sodium salicylate and sodium citrate inhibit the sulfotransferase activity noncompetitively, and inhibition of cholesterol sulfate formation may be important in the desquamative action of these topical "keratolytics."
D000818:Animals; D000831:Animals, Newborn; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003600:Cytosol / Q000201:enzymology; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects / Q000201:enzymology*; D066298:In Vitro Techniques; D007641:Keratolytic Agents / Q000494:pharmacology; D051379:Mice; D012980:Sodium Salicylate / Q000494:pharmacology; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism
D002788:Cholesterol Esters; D002951:Citrates; D007641:Keratolytic Agents; D019343:Citric Acid; D003687:Dehydroepiandrosterone; D002784:Cholesterol; D013466:Sulfurtransferases; D015238:Sulfotransferases; C043456:cholesterol sulfotransferase; C007045:cholesteryl sulfate; D012980:Sodium Salicylate