Biocompatibility screening of poly (vinyl chloride) implants.
It was proposed to test the biocompatibility of a polymeric implant by assaying enzyme activity in the tissue homogenate. Medical grade poly(vinyl chloride) was implanted in rat gluteal muscle and muscle specimens taken from the implanted and non-implanted sites after 7, 14 and 21 days. In the muscle homogenate taken in suitable solvents, the activities of alkaline phosphatase, acid phosphatase, Ca2+ ATPase, leucine aminopeptidase (LAP) and succinic dehydrogenase (SDH) were tested and compared at implanted and non-implanted sites. Increased activity of acid phosphatase, alkaline phosphatase, LAP and Ca2+ ATPase were seen at the implanted site but the SDH activity was reduced. The enzyme activity at both sites was maximum around 14 days and the highest increase in activity at the implant site compared to the control site was observed for alkaline phosphatase and acid phosphatase. After 21 days, the activity decreased gradually. In case of SDH, the inhibition was more after 14 days and gradually decreased after 21 days.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D011143:Polyvinyl Chloride; D011145:Polyvinyls; D019736:Prostheses and Implants; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
D001672:Biocompatible Materials; D011145:Polyvinyls; D011143:Polyvinyl Chloride; D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase; D000252:Calcium-Transporting ATPases