High-performance liquid chromatography of pefloxacin and its main active metabolites in biological fluids.
We describe a high-performance liquid chromatographic (HPLC) method for the analysis of pefloxacin, a new antibacterial agent, in plasma and urine following administration of a therapeutic dose in humans. HPLC assay of pefloxacin and its two main active metabolites in urine is also described. The applicability of the methods to pharmacokinetic studies of pefloxacin in humans is demonstrated.
D000890:Anti-Infective Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009268:Nalidixic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D009643:Norfloxacin / Q000031:analogs & derivatives; D015366:Pefloxacin; D013997:Time Factors
D000890:Anti-Infective Agents; D015366:Pefloxacin; D009268:Nalidixic Acid; C037271:oxonorfloxacin; D009643:Norfloxacin