Vanadate uptake in Neurospora crassa occurs via phosphate transport system II.
Vanadate, a potent inhibitor of plasma membrane ATPases, is taken up by Neurospora crassa only when cells are growing in alkaline medium and starving for phosphate. The appearance of a vanadate uptake system (Km = 8.2 microM; Vmax = 0.15 mmol/min per liter of cell water) occurs under the same conditions required for derepression of a high-affinity phosphate transport system. Phosphate is a competitive inhibitor of vanadate uptake, and vanadate is a competitive inhibitor of phosphate uptake. Furthermore, mutant strains which are either partially constitutive or non-derepressible for the high-affinity phosphate transport system are also partially constitutive or non-derepressible for vanadate uptake. These data indicate that vanadate enters the cell via phosphate transport system II.
D001692:Biological Transport; D003470:Culture Media; D004983:Ethanolamines / Q000494:pharmacology; D005809:Genes, Regulator; D007700:Kinetics; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D014638:Vanadates; D014639:Vanadium / Q000378:metabolism*
D003470:Culture Media; D004983:Ethanolamines; D010710:Phosphates; D014639:Vanadium; D014638:Vanadates; C005448:phosphorylethanolamine