Stabilization of the amplification convertase of complement by monoclonal antibodies directed against human factor B.
Three IgM mouse monoclonal antibodies (MoAb), 5B5-A, 2D2-B, and 5B12-A, were prepared by fusion of spleen cells from mice immunized with human B with the SP 2/0 myeloma cell line. They were assessed for their effect on cell-bound and fluid-phase amplification convertases of complement (C) with purified proteins in vitro. 5B5-A and 2D2-B were similar in their effects on cell-bound preformed C3bBb in that they bound to cell-bound C3bBb, stabilized the C3bBb convertase, and rendered the C3bBb convertase relatively resistant to the plasma protein H. These two MoAb were also able to enhance C3 consumption in vitro in reaction mixtures containing C3b, C3, B, and D. At the same time, they presumably stabilized the C3 convertase and caused relative sparing of B hemolytic activity in the reaction mixtures. In contrast, 5B12-A, which also bound to Bb and C3bBb, did not induce any stabilization, but rather caused accelerated decay of cell-bound C3bBb. These results indicate that MoAb against B can have C3NeF-like activity. On the other hand, not all MoAb against B have stabilizing activity on the C3bBb convertase.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001666:Binding Sites, Antibody; D003166:Complement Activating Enzymes / Q000378:metabolism*; D003167:Complement Activation; D003178:Complement C3 Nephritic Factor / Q000378:metabolism; D050577:Complement C3-C5 Convertases / Q000378:metabolism*; D003180:Complement C3b Inactivator Proteins / Q000502:physiology; D011415:Complement Factor B / Q000276:immunology*; D017242:Complement Factor H; D003170:Complement Pathway, Alternative; D004792:Enzyme Precursors / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism; D006041:Goats; D006801:Humans; D051379:Mice; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b
D000911:Antibodies, Monoclonal; C493495:CFH protein, human; D003178:Complement C3 Nephritic Factor; D003180:Complement C3b Inactivator Proteins; D004792:Enzyme Precursors; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D017242:Complement Factor H; D003166:Complement Activating Enzymes; D050577:Complement C3-C5 Convertases; D011415:Complement Factor B