[Isolation of tissue-specific inhibitors of DNA synthesis (chalones) from rat liver].
Two fractions of non-cytotoxic and tissue-specific inhibitors of 3H-thymidine incorporation into DNA of rat liver regenerating after partial hepatectomy in vitro were obtained by gel filtration on a Sephadex-75 column of the ethanol precipitated aqueous extract. One of these fractions (Ve/Vo = = 2.0; Kav = 0.4) was found to be heterogenous and enriched with proteins with a molecular weight of 17 000-30 000 D. It was biologically active at concentrations exceeding 0.25 mg/ml. The biological activity of the other fraction (Ve/Vo = 3.0; Kav = 1) occurred at concentrations under 0.005 mg/ml. The main component of this fraction was discovered to be a protein with a molecular weight of 17 000 D.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000032:analysis*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000378:metabolism
D006131:Growth Inhibitors; D004247:DNA
Vydelenie tkanespetsificheskikh ingibitorov sinteza DNK (keĭlonov) iz pecheni krys.