title: Calcium transport and fluorescence polarization of 1,6-diphenyl-1, 3,5-hexatriene in sarcoplasmic reticulum from normal and ethanol-tolerant rats.
abstract: Sarcoplasmic reticulum (SR) of high purity and functional integrity was isolated from skeletal muscle of normal and ethanol-tolerant rats. Ethanol at low concentrations (0.1 to 0.2 M), added in vitro to isolated SR, resulted in slight inhibition of both calcium loading and calcium-stimulated ATPase rates. Higher concentrations of ethanol resulted in further inhibition of calcium loading, but not of calcium-stimulated ATPase. Fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) in isolated SR membranes showed a small decrease in anisotropy with the addition of ethanol in vitro. Sarcoplasmic reticulum from normal and ethanol-tolerant rats did not differ in calcium pump function or in fluorescence polarization of DPH, in the presence or absence of ethanol.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D004161:Diphenylhexatriene / Q000378:metabolism*; D004361:Drug Tolerance; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D011090:Polyenes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D011090:Polyenes; D004161:Diphenylhexatriene; D000431:Ethanol; D000251:Adenosine Triphosphatases; D002118:Calcium