[Expression of hepatitis B virus (HBV) genome in the oocytes of two species of amphibians].
Xenopus laevis and Pleurodeles waltlii oocyte nuclei are injected with a recombinant plasmid pCP 10, carrying two hepatitis B virus (HBV) genomes in a tandem arrangement. Transcription occurs in oocytes of both species and production of viral RNAs is demonstrated; better quantitative results are obtained with P. waltlii oocytes. Two viral proteins are detected: Hepatitis B surface antigen (Ag HBs) and Hepatitis B core antigen (Ag HBc). These proteins can be shown only in oocyte homogenates, and not in the incubation media. Another viral marker (Ag HBe) was not detected.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism; D005260:Female; D005796:Genes; D005814:Genes, Viral; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism*; D010957:Plasmids; D010999:Pleurodeles; D013045:Species Specificity; D014158:Transcription, Genetic; D014981:Xenopus
D004274:DNA, Recombinant; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens
Expression du génome du virus de l'hépatite B (HBV) dans les ovocytes de deux espèces d'amphibiens.