Aclacinomycin A-inhibition of phage phi X174 DNA synthesis in vitro.
Aclacinomycin A inhibited the in vitro conversion of phage phi X174 single-stranded DNA to the replicative form DNA. DNA synthesis was inhibited by 50% in the presence of 15 microM aclacinomycin A. The inhibition was competitive with respect to template DNA (Ki = 13 microM) and was reversed by addition of Escherichia coli cell extracts. Short complementary strands approximately one-third of unit length molecule were synthesized in the presence of 15 microM aclacinomycin A. The data suggest that aclacinomycin A may inhibit the process of phi X174 DNA chain elongation by a direct interaction with the E. coli host enzymes.
D015250:Aclarubicin; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D010584:Bacteriophage phi X 174 / Q000187:drug effects / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D009279:Naphthacenes / Q000494:pharmacology; D013698:Templates, Genetic; D014779:Virus Replication / Q000187:drug effects
D000900:Anti-Bacterial Agents; D004277:DNA, Single-Stranded; D009279:Naphthacenes; D015250:Aclarubicin