Negative feedback regulation of contact sensitivity to DNFB by autoanti-idiotypic antibody.
Contact sensitivity to 2,4-dinitrofluorobenzene is maximal six days after sensitization but declines rapidly, due to autoanti-idiotypic antibodies produced by the host. The studies presented here indicate that this down regulation by anti-Id is a C-independent active process involving a subset of Ia+ T cells in the immune lymph node cell population. Depleting immune LN cells of Ia+ T cells renders them insensitive to inhibition by anti-Id alone, although the same population is inhibited by treatment anti-Id plus C. This cell population is rendered sensitive to inhibition by anit-Id alone by adding untreated DNFB-sensitized LN cells but not by adding normal LN cells. Further studies showed that suppression by anti-Id-activated Ia+ T cells occurs locally at the skin test site and is antigen nonspecific. These data indicate that the natural regulation of CS to DNFB by autoanti-Id antibodies involves a negative feedback regulatory loop.
D000818:Animals; D000961:Antilymphocyte Serum / Q000008:administration & dosage; D001323:Autoantibodies / Q000096:biosynthesis* / Q000276:immunology; D003165:Complement System Proteins / Q000378:metabolism; D003877:Dermatitis, Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology*; D005246:Feedback; D005802:Genes, MHC Class II; D007108:Immune Tolerance; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009578:Nitrobenzenes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000961:Antilymphocyte Serum; D001323:Autoantibodies; D007130:Immunoglobulin Idiotypes; D009578:Nitrobenzenes; D003165:Complement System Proteins; D004139:Dinitrofluorobenzene