Large-scale isolation of human erythrocyte Ca2+-transport ATPase.
A rapid procedure for preparing large quantities of purified erythrocyte Ca2+-transport ATPase is presented. The method involves: (1) fast preparation of calmodulin-deficient, essentially haemoglobin-free, erythrocyte membranes by molecular filtration using Pellicon filters; (2) solubilization of membrane proteins by deoxycholate; and (3) a batch procedure using calmodulin-Sepharose 4B gel for purification of Ca2+-transport ATPase.
D000252:Calcium-Transporting ATPases / Q000097:blood / Q000302:isolation & purification*; D002458:Cell Fractionation; D002498:Centrifugation; D002850:Chromatography, Gel; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008722:Methods; D014462:Ultrafiltration
D000252:Calcium-Transporting ATPases