[Effect of active immunization against 17 beta-estradiol on cytosolic estrogen receptors in the rat uterus].
Following active immunization of female rats against estradiol-17 beta, the amount of specific binding sites for estrogen decreased in uterine cytosol as a function of antiserum titres. They were undetected when antibodies titres were higher than 1/2000. Moreover, a binding protein specific for estradiol-17 beta appeared. Estradiol binding was not displaced with an excess of unlabeled DES nor precipitated with protamine sulfate. The sedimentation coefficient of the hormone-protein complex (7-8 S) was not modified in medium of high ionic strength (0.4 M KCl). That protein represented antibodies to Estradiol-17 beta which could be precipitated with antiserum to rat IgG.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D004958:Estradiol / Q000276:immunology*; D005260:Female; D007114:Immunization; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000032:analysis*; D014599:Uterus / Q000032:analysis*
D000906:Antibodies; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D004958:Estradiol
Effets de l'immunisation active contre le 17 beta-oestradiol sur les récepteurs cytosoliques des oestrogènes dans l'utérus de ratte.