[Effects of insulin, somatomedin--a multiplication stimulating activity (MSA)--and transferrin, on the lipolysis of rat adipocytes in vitro].
Antilipolytic effect was researched when insulin (0.1 and 1 mIU/ml), MSA (200 and 500 ng/ml) and transferrin (2 and 5 micrograms/ml) were added to a suspension of freshly isolated rat adipocytes in vitro. Lipolysis was measured as glycerol secretion in the medium: micromoles/90 minutes/100 mg total lipids. Insulin (1 mIU/ml) reduced adrenalinic stimulation of lipolysis: A 1 microgram/ml (P less than 0.05). MSA 200 ng/ml had no effect. MSA 500 ng/ml reduced basal lipolysis and adrenalinic stimulation (P less than 0.05), and increased insulin-induced antilipolysis (P less than 0.05). Transferrin was active, only when insulin is present: antilipolysis increased (P less than 0.05).
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005990:Glycerol / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II; D008066:Lipolysis / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000494:pharmacology*; D014168:Transferrin / Q000494:pharmacology*
D007328:Insulin; D010455:Peptides; D013002:Somatomedins; D014168:Transferrin; D007335:Insulin-Like Growth Factor II; D005990:Glycerol; D004837:Epinephrine
Effects de l'insuline, d'une somatomédine--facteur de multiplication cellulaire (MSA)--et de la transferrine, sur la lipolyse de l'adipocyte de rat in vitro.