Heparan sulfate--rich anionic sites in the human glomerular basement membrane. Decreased concentration in congenital nephrotic syndrome.
Recent work suggests that the normal barrier to penetration of the renal glomerular basement membrane by anionic plasma proteins may depend in part on the existence of negatively charged sites within the membrane. We describe an in vitro cytochemical method for the quantitative demonstration of anionic sites in the normal human glomerular basement membrane. In five normal subjects, ranging in age from 10 days to 57 years, the sites were distributed at regular intervals in the lamina rara externa, with a frequency of 23.8 +/- 6.8 sites per 1000-nm length of membrane. A similar distribution was observed in the basement membranes from three normal human fetuses. Ex vivo perfusion of one cadaver kidney revealed a similar distribution of anionic sites. The number of anionic sites in the glomerular basement membranes of five patients with the congenital nephrotic syndrome was reduced to 8.9 +/- 3.7 (P less than 0.001). Prior incubation of sections of normal kidney in purified heparinase resulted in a marked reduction in the number of anionic sites. We conclude that congenital nephrosis results from failure of heparan sulfate--rich anionic sites to develop in the lamina rara externa of the glomerular basement membrane.
D000838:Anions; D001485:Basement Membrane / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D002675:Child, Preschool; D005333:Fetus / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D019763:Heparin Lyase; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007678:Kidney Glomerulus / Q000032:analysis / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D009404:Nephrotic Syndrome / Q000151:congenital* / Q000378:metabolism; D010477:Perfusion; D011094:Polyethyleneimine / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000494:pharmacology; D011507:Proteinuria / Q000209:etiology
D000838:Anions; D006025:Glycosaminoglycans; D019763:Heparin Lyase; D006497:Heparan Sulfate; D011094:Polyethyleneimine; D011133:Polysaccharide-Lyases