The effect of glucose on the activity of phosphofructokinase in the mucosa of rat small intestine.
In common with other phosphofructokinase isoenzymes, phosphofructokinase in the epithelial cells of rat small-intestinal mucosa is activated by fructose 2,6-bisphosphate. However, fructose 2,6-bisphosphate was found not to be present in mucosa as judged by three criteria: (1) chromatography on Sephadex G-25 of crude mucosal extracts from fed rats did not result in a decrease, or indeed any change, in the activity of phosphofructokinase under suboptimal conditions at pH7; (2) ultrafiltrates of mucosal extracts did not possess any acid-labile activating activity when tested against chromatographed liver phosphofructokinase; (3) phosphofructokinase-2 activity was not detectable in mucosal extracts. Furthermore, the perfusion in vitro of isolated loops of jejunum or the incubation of mucosal scrapings from either fed rats or rats starved for 48 h showed that the activity of mucosal phosphofructokinase is not subject to short-term regulation by glucose. These observations are consistent with the view that phosphofructokinase is the rate-limiting enzyme of glycolysis in intestinal mucosa and account for the fact that the rate of glucose utilization by rat small intestine is not very responsive to changes in the concentration of glucose in the lumen.
D000818:Animals; D002850:Chromatography, Gel; D004848:Epithelium / Q000201:enzymology; D005636:Fructosephosphates / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D051381:Rats; D013217:Starvation / Q000201:enzymology
D005636:Fructosephosphates; C027618:fructose-6-phosphate; D010732:Phosphofructokinase-1; D005947:Glucose