Effect of indomethacin on Ca2+-stimulated adenosine triphosphatase in the synaptic vesicles of rat brain in vitro.
1. Indomethacin inhibits calcium-stimulated adenosine triphosphatase (Ca2+-ATPase), calcium, magnesium-stimulated adenosine triphosphatase (Ca2+,Mg2+-ATPase) and magnesium-stimulated adenosine triphosphatase (Mg2+-ATPase) activities in rat brain synaptic vesicles in vitro. 2. The Ca2+-ATPase activity is most strongly affected by this drug all of the activities of ATPases tested. 3. The decrease of Ca2+-ATPase activity by addition of indomethacin is due to a decrease of Vmax. 4. The Ki values for this drug for ATP and Ca2+ in Ca2+-ATPase were 1.13 mM and 0.68 mM, respectively.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008297:Male; D051381:Rats; D013572:Synaptic Vesicles / Q000201:enzymology
D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D002118:Calcium; D007213:Indomethacin