Monoclonal anti-(T,G)-A--L antibodies: characterization of fine specificity and idiotype expression.
The technique of polyethylene glycol mediated cell fusion was used to establish 22 monoclonal cell lines secreting anti-(T,G)-A--L antibody. Cell lines were derived from C3H.SW and B10 mice and produced antibody with kappa light chains and predominantly gamma 1, heavy chains. Fine-specificity analysis demonstrated that 15 cell lines made antibodies that also recognize a determinant present on GAT, GT (9:1) and GT (1:1), whereas little, if any, serum antibody demonstrates this cross-reaction. Fourteen antibodies, derived from both B10 and C3H.SW mice, bear idiotypic determinants defined by Lewis anti-[B10 anti-(T,G)-A--L], but only two, both from C3H.SW mice, react with Lewis anti-[C3H.SW anti-(T,G)-A--L]. Adsorption studies indicate that no hybridoma tested bore the complete set of idiotypic determinants defined by either serum.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007163:Immunosorbent Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010455:Peptides; C021759:(T,G)-A-L