[Accumulation of exogenous monoamines in the notochord of lampreys: fluorescence microscopy and radiography study].
Storage of biogenic amines was studied in the notochord of the lamprey (Lampetra planeri) after injection of cold or tritiated amines or their precursors. After injection of dopamine, noradrenaline or 5-hydroxytryptophane, an intense formaldehyde-induced fluorescence was observed in the cytosol of chordal cells and in the perichordal sheat. A similar location occurred after injection of the same tritiated products. No qualitative or significant quantitative differences could be distinguished between the larval and the metamorphosing forms. RO4-4602, a decarboxylase inhibitor, and Nialamide, a monoamine-oxydase inhibitor, did not modify significantly the incorporation of 3H-5-hydroxytryptophan. Using histochemical technique a monoamine oxydase activity could be detected in the nervous system and in the muscle but was not shown in the notochord.
D000818:Animals; D001345:Autoradiography; D001679:Biogenic Amines / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D007798:Lampreys / Q000033:anatomy & histology / Q000378:metabolism*; D008856:Microscopy, Fluorescence
D001679:Biogenic Amines
Accumulation de monoamines exogènes dans la corde de la lamproie de Planer: étude en microscopie de fluorescence et en radioautographie.