Acrylic microspheres in vivo V: Immunological properties of immobilized asparaginase in microparticles.
L-Asparaginase was immobilized in microparticles of polyacrylamide. Such particles were then injected by intramuscular/subcutaneous, intraperitoneal, or intravenous routes into mice to investigate the immunological consequences of the immobilization. Entrapment of L-asparaginase in microparticles did not prevent the formation of antibodies in intensively treated animals. Intraperitoneal and intravenous injections of particles produced significantly higher antibody levels than soluble L-asparaginase. Antigen administered intramuscularly/subcutaneously in microparticles elicited, however, a weak immune response. Dependent on the route of administration, the particles may thus function as an adjuvant. A modified Arthus reaction in the foot pads of immunized mice indicated that antigenicity decreased when L-asparaginase was immobilized in microparticles. Injection of free L-asparaginase, intramuscularly/subcutaneously (2 x 20 IU) in the preimmunized mice produced no effects on the serum level of L-asparagine, whereas intramuscular/subcutaneous injection of L-asparaginase in microparticles produced a depression of the serum concentration. It is concluded that the intramuscular/subcutaneous injection of L-asparaginase in microparticles is the choice route of administration with respect to duration and the immunological reaction.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000187:drug effects; D001183:Arthus Reaction / Q000276:immunology; D001215:Asparaginase / Q000008:administration & dosage / Q000276:immunology*; D001216:Asparagine / Q000097:blood; D001345:Autoradiography; D004800:Enzymes, Immobilized / Q000008:administration & dosage / Q000276:immunology*; D008297:Male; D051379:Mice; D008863:Microspheres; D014018:Tissue Distribution
D000906:Antibodies; D004800:Enzymes, Immobilized; D001216:Asparagine; D001215:Asparaginase