Isolation of nonlysogenic bacteria from lambda-lysogenized strains using antibiotic-resistant phage.
A simple and efficient method for obtaining nonlysogenic bacteria from lambda-lysogenized strains using phage with an inserted antibiotic-resistant plasmid is described. When the lysogenic culture is infected with antibiotic-resistant phage, single non-lysogenic cells are lysogenized, isolated on selective medium, and cured of phage during incubation at 37 degrees C.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000302:isolation & purification*; D010582:Bacteriophage lambda / Q000187:drug effects*; D004352:Drug Resistance, Microbial; D008242:Lysogeny
D000900:Anti-Bacterial Agents