title: Differential survival and chloramphenicol-insensitive error-prone repair of hydroxylamine-inactivated phi X174 bacteriophage mutants.
abstract: Features of inactivation, repair and concomitant mutagenesis of hydroxylamine-treated phi X174 bacteriophages are reported here. (1) For reasons unknown, the nonsense phage mutants tested here were far more sensitive to hydroxylamine than the wild-type phage. In contrast, the sensitivities of these same phi X174 mutants to UV-irradiation are indistinguishable. (2) Hydroxylamine-treated amber phages mutated to ochre but not to wild-type particles, i.e., G leads to A transition events were recovered. (3) The repair of phi X174 phages from hydroxylamine-induced damage was error-prone, but unlike UV damage, did not require protein synthesis de novo. Possible mechanisms of these novel features are discussed.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D002701:Chloramphenicol / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects; D006898:Hydroxylamines / Q000494:pharmacology*; D009154:Mutation; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D006898:Hydroxylamines; D002701:Chloramphenicol