Differentiation of pathogenic and saprophytic leptospira strains.
Comparative studies of 249 pathogenic and 80 saprophytic leptospira strains, including 2 strains of the illini type, using the 8-azaguanine test, growth at 13 degrees C and growth on trypticase soy broth revealed their good differentiating potency if the recommended conditions were carefully observed. The same results were obtained by a simple hemolytic test using sheep and rat blood cells, having the advantage of providing results within 24 h. This test is suggested to replace the 8-azaguanine and the growth test at 13 degrees C. In these investigations, the first European strain of the illini type was recognized.
D000818:Animals; D001375:Azaguanine; D002364:Caseins; D003470:Culture Media; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis; D007919:Leptospira / Q000145:classification* / Q000254:growth & development / Q000472:pathogenicity; D011492:Protein Hydrolysates; D051381:Rats / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013696:Temperature
D002364:Caseins; D003470:Culture Media; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D001375:Azaguanine