The in vivo regulation of splenic T cell population by the prostaglandin-mediated system.
The effects of prostaglandins (PGs) on splenic T cell population, the generation of Con A-induced suppressor T cells, and antibody response to sheep erythrocytes were examined in mice in which the in vivo level of PG was regulated by indomethacin (INDO), an inhibitor of PG-synthesis, and exogenous PGE2. Inhibition of PG-synthesis enlarged the T cell population in the spleen and such an enlargement was due to an increase in Lyt-1+2+ and Lyt-1-2+ subsets. In this group, the induction of suppressor T cells was augmented, while the antibody response was suppressed. Elevation of the PG-level scarcely affected the size of the whole T cell population in the spleen but slightly increased a Lyt-1+2- subset. The production of suppressor T cells was disturbed, and the antibody response was augmented in this T cell population. Experiments using anti-Lyt antibody plus complement showed that Lyt-1+2+ subsets exhibited these distinctive activities between PG-decreased and PG-increased mice. A Lyt-1+2+ subset from mice with a high PG-level may be in an advanced stage of maturation compared with that from mice with a low PG-level under the promoting effect of PG on T cell differentiation. In vivo regulation of the PG-level appears to play an important role in the regulation of the T cell population and immune response.
D000818:Animals; D000917:Antibody Formation; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D015232:Dinoprostone; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000276:immunology; D010641:Phenotype; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000954:Antigens, Surface; D008934:Mitogens; D011453:Prostaglandins; D011458:Prostaglandins E; D015232:Dinoprostone; D007213:Indomethacin