title: Altered property of sarcoplasmic Ca-ATPase from vitamin E-deficient dystrophic rabbit is associated with the protein and not the lipid component.
abstract: The effects of temperature on reconstituted sarcoplasmic Ca-ATPase preparations from vitamin E-deficient dystrophic and control rabbits were studied. Delipidated Ca-ATPase from vitamin E-deficient sarcoplasmic reticulum (SR) reconstituted with lipid of control SR exhibited properties similar to preparations reconstituted with lipid of vitamin E-deficient SR, namely low Ca-ATPase activity and a linear Arrhenius plot of enzyme activity. On the other hand, delipidated control SR Ca-ATPase reconstituted with lipid of vitamin E-deficient SR showed a reduction in activity but retained the discontinuity in the Arrhenius plot. These results indicated that the altered property of sarcoplasmic Ca-ATPase from vitamin E-deficient dystrophic rabbit was associated with the protein and not the lipid component.
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis / Q000378:metabolism*; D004032:Diet; D007700:Kinetics; D050356:Lipid Metabolism; D009132:Muscles / Q000201:enzymology; D009137:Muscular Dystrophy, Animal / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014811:Vitamin E Deficiency / Q000201:enzymology*
chemical_list: D011506:Proteins; D000252:Calcium-Transporting ATPases