title: Rosette inhibitory factor: T-lymphocyte subpopulation specificity and potential immunoregulatory role in hepatitis B virus infection.
abstract: We have observed the disappearance of rosette inhibitory factor (RIF) from the serum of 19 patients with acute hepatitis B virus infection. This occurred at a time coinciding with the detection of anti-HBs. In addition, levels of RIF activity were significantly greater (P less than 0.001) in 35 HBsAg carriers who lacked anti-HBs when compared to 15 carriers who regularly demonstrated this antibody. In all instances, RIF effect was partial affecting some, but not all, T-lymphocytes from healthy donors were separated into TM (helper), TG (suppressor), and T0 (null) subpopulations by an immunoglobulin-ox-cell rosette depletion method. The effect of RIF on erythrocyte rosette formation and Fc-receptor expression in these subpopulations was assessed. TG-lymphocytes were found to be refractory to RIF-mediated suppression of erythrocyte rosette formation while TM-lymphocytes demonstrated an enhanced sensitivity to RIF. Incubation of TG-lymphocytes, potential TM-percursor cells, with RIF resulted in a decreased expression of new IgM-Fc receptors. In order to determine if any functional significance could be derived from these findings, the effect of RIF on in vitro immunoglobulin secretion was tested. Using pokeweed mitogen-stimulated mononuclear cell cultures, purified RIF-low density lipoprotein was shown to suppress IgM, IgG, and IgA secretion by 75.3, 74.3, and 59.3%, respectively. These data are consistent with the hypothesis that RIF is a potential immunoregulatory protein which could contribute to the lack of anti-HBs noted during the acute phase of hepatitis B and in the majority of HBsAg carriers.
mesh_terms: D001798:Blood Proteins; D006509:Hepatitis B / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D008077:Lipoproteins, LDL; D008215:Lymphocytes, Null / Q000276:immunology; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000378:metabolism; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D001798:Blood Proteins; D007136:Immunoglobulins; D008077:Lipoproteins, LDL; D017452:Receptors, IgG; D011971:Receptors, Immunologic; C022060:rosette-inhibiting factor