Neutrophil migration and orientation under agarose: findings in patients with the immotile cilia syndrome and effects of cytochalasin B and vinblastine.
This study has shown that the agarose technique is well suited for determination of whether impaired PMN migration is due to defective direction finding of deficient locomotive ability. By assessments of the degree of orientation of neutrophils together with measures of migration distances, we demonstrated that vinblastine treated normal neutrophils and PMNs from patients with the immotile cilia syndrome mainly move in a chemokinetic manner, whereas cytochalasin B hampers locomotive ability, but leaves direction finding intact. Hence, information whether chemotaxis or chemokinesis predominates is easily obtained in one single assay system.
D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002923:Cilia / Q000502:physiology; D003470:Culture Media; D003571:Cytochalasin B / Q000494:pharmacology*; D004398:Dyneins / Q000502:physiology; D006801:Humans; D008870:Microtubules / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D012685:Sepharose; D014747:Vinblastine / Q000494:pharmacology*
D003470:Culture Media; D003571:Cytochalasin B; D014747:Vinblastine; D012685:Sepharose; D004398:Dyneins