title: EXAFS investigation of the binuclear cupric site in met T2D Rhus laccase and its azide bound derivative.
abstract: EXAFS analysis of met T2D Rhus laccase and its azide bound derivative indicates an average of 0.33 S at 2.09 A and 3-4 N (or O) atoms at 2.00 A per copper atom for the three copper centers. Using the plastocyanin Cu(II) EXAFS spectrum to model the type 1 site in laccase, a difference EXAFS spectrum for the type 3 site is generated; this spectrum enables assignment of the one S ligand in met T2D to the type 1 site and indicates no evidence of a detectable copper scatterer for the coupled binuclear copper site. Implications regarding type 3 optical features and related studies on the hemocyanins are also discussed.
mesh_terms: D001386:Azides; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper / Q000302:isolation & purification*; D006433:Hemocyanins; D042845:Laccase; D008715:Methionine / Q000378:metabolism; D010088:Oxidoreductases; D010947:Plants, Toxic; D013057:Spectrum Analysis; D012251:Toxicodendron / Q000201:enzymology*; D014965:X-Rays
chemical_list: D001386:Azides; D003300:Copper; D006433:Hemocyanins; D008715:Methionine; D010088:Oxidoreductases; D042845:Laccase