title: Comparative study on the immunosuppressive and lympholytic activity of optical isomers of cyclophosphamide.
abstract: The influence of chirality of cyclophosphamide (Cy) on the reactivity of lymphoid cells in vivo was studied in Balb/c mice. It has been shown that (+) Cy inhibits the formation of direct PFC in Balb/c mice more effectively than (-) Cy. The augmentation of DTH--reaction against HuRBC in mice, measured by the foot-pad test and the elongation of graft survival time were noted when racemic or (-) Cy were applied. Investigated forms of Cy decreased the number of nucleated cells in mice spleens, but lympholytic effect of (+) Cy was lower than that of racemic and (-) Cy. The possible consequences of stereo-differentiated immunosuppressive activity of enantiomeric forms of cyclophosphamide in the experimental procedures and the therapy of "autoimmune" and malignant diseases are considered.
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D006968:Hypersensitivity, Delayed; D007166:Immunosuppressive Agents; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013237:Stereoisomerism; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects
chemical_list: D007166:Immunosuppressive Agents; D003520:Cyclophosphamide