Isotype specificity of helper T cell clones. Peyer's patch Th cells preferentially collaborate with mature IgA B cells for IgA responses.
The nature of the IgA B cell precursors that receive preferential help from selected clones of T helper cells from mouse Peyer's patches (PP Th A) were studied. Activation of the PP Th A clones required the presence of antigen, sheep erythrocytes (SRBC), in a culture system supporting development of antibody-secreting plasma cells. Two types of PP Th A cells were used. Both gave vigorous IgA responses; the first also supported low IgM, and the second low IgM and IgG subclass antibody responses. Removal of sIgA+ B cells from either splenic or PP B cell cultures selectively depleted precursors of IgA antibody producers. Cultures of purified sIgA+ B cells, cloned PP Th A cells and SRBC, selectively yielded IgA antibody producers. Finally, PP Th A cells did not support IgA responses in B cell cultures derived from spleens of young mice (days 1-25), and full IgA responses were not seen until the donor mice were 6-7 wk of age. These results suggest that cloned T helper cells can recognize and collaborate with mature, IgA committed B cells.
D000818:Animals; D000921:Antibody-Producing Cells / Q000145:classification / Q000166:cytology / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D002999:Clone Cells / Q000276:immunology; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D008211:Lymphocyte Cooperation; D051379:Mice; D008809:Mice, Inbred C3H; D010581:Peyer's Patches / Q000166:cytology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D007070:Immunoglobulin A; D007126:Immunoglobulin Allotypes; D011947:Receptors, Antigen, B-Cell