Roles of thymidylate synthetase activity in herpes simplex virus-infected HeLa cells.
Thymidylate synthetase was not essential for herpes simplex virus replication. However, it appeared to contribute to the formation of a portion of thymidine nucleotides for DNA synthesis in virus-infected cells. Therefore, it is possible that the enzyme plays an important role in determining the potency of several selective antiviral thymidine analogs. Since synergistic effects of 5-fluoro-2'-deoxyuridine with other analogs on virus reproduction were observed, it is suggested that the effects of these analogs depend to a certain degree on the abilities of the monophosphate derivatives to inhibit thymidylate synthetase.
D004357:Drug Synergism; D005467:Floxuridine / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000201:enzymology; D006561:Herpes Simplex / Q000201:enzymology*; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism*; D009705:Nucleosides / Q000494:pharmacology; D013940:Thymidylate Synthase / Q000378:metabolism*
D009705:Nucleosides; D005467:Floxuridine; D008780:Methyltransferases; D013940:Thymidylate Synthase