Cytochemistry of human T lymphocyte subpopulations in peripheral blood.
With sequential investigation of immunological and cytochemical markers on single cells, T lymphocyte subsets defined by monoclonal antibodies were examined in the peripheral blood of healthy adults. Leu-3a+ T cells (helper/inducer) were 80.0 +/- 6.6% dot positive with unspecific acid alpha-naphthyl acetate esterase (ANAE) and 52.2 +/- 15.8% dot positive with diaminopeptidase IV (DAP IV) staining, as compared to 52.6 +/- 7.6% ANAE and 29.1 +/- 10.9% DAP IV dot positivity in Leu-2a+ T cells (suppressor/cytotoxic). Although these differences were significant (P less than 0.001 and P = 0.002, respectively), the rather high % of dot positivity in Leu-2a+ cells precludes ANAE or DAP IV staining as a simple method for the determination of T lymphocyte subsets. In acid phosphatase staining, no significant difference in focal positivity between Leu3a+ and Leu-2a+ T cell subpopulations was detected (63.3 +/- 10.9% and 52.3 +/- 10.0%, respectively; P greater than 0.1).
D000135:Acid Phosphatase; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D008875:Middle Aged; D009283:Naphthol AS D Esterase; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification* / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000911:Antibodies, Monoclonal; D009283:Naphthol AS D Esterase; D000135:Acid Phosphatase