High mobility group proteins HMG1 and HMG2 do not decrease the melting temperature of DNA.
High mobility group proteins 1 and 2 isolated in non-denaturing conditions cannot decrease the temperature of denaturation of DNA. When they are isolated or treated with tricloroacetic acid a hyperchromic peak below the melting temperature of free DNA appears in agreement with previous data ( Javaherian et al. (1979) Nucl . Acids Res. 6, 3569-3580). We show that this is due to light scattering of aggregated protein at submelting temperatures and not to melting of DNA.
D000818:Animals; D002417:Cattle; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA; D006609:High Mobility Group Proteins; D009691:Nucleic Acid Denaturation; D013816:Thermodynamics
D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D004247:DNA