Identification of an essential lysine residue in the beta subunit of the F1-ATPase from the thermophilic bacterium, PS3, using 7-chloro-4-nitro[14C]benzofurazan.
When the F1-ATPase from the thermophilic bacterium, PS3, was inactivated by 90% with 7-chloro-4-nitro[14C]benzofurazan ([14C]Nbf-Cl) at pH 7.3 and then gel-filtered, 1.25 mols of [14C]Nbf-O-Tyr and less than 0.1 mol of Nbf-N-Lys were formed per mol of enzyme. After adjusting the pH of the gel-filtered, modified enzyme to 9.0 and incubating it for 14 hrs. at 23 degrees C to promote O----N migration, 0.68 mol of Nbf-N-Lys were formed per mol of enzyme while about 16% of the original activity reappeared. Isolation of the subunits after the O----N migration showed that 90% of the incorporated 14C was present in the beta subunit, which contained 0.21 mols of [14C]Nbf-N-Lys per mol. A tryptic peptide which contained the majority of the 14C incorporated into the beta subunit was isolated and subjected to automatic amino acid sequence analysis contained 38 residues. The amino acid sequence immediately around the lysine residue labeled with [14C]Nbf-, K*, was found to be: ...I-G-L-F-G-G-A-G-V-G-K*-T-V-L-I-G... .
D009327:4-Chloro-7-nitrobenzofurazan / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D002250:Carbon Radioisotopes; D006358:Hot Temperature; D008239:Lysine / Q000032:analysis*; D046911:Macromolecular Substances; D010069:Oxadiazoles / Q000494:pharmacology*; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D014357:Trypsin
D002250:Carbon Radioisotopes; D046911:Macromolecular Substances; D010069:Oxadiazoles; D010446:Peptide Fragments; D014357:Trypsin; D006180:Proton-Translocating ATPases; D009327:4-Chloro-7-nitrobenzofurazan; D008239:Lysine