Autologous mixed lymphocyte reaction in man: further characterization of responding cells.
Experiments were performed to determine the type of cells undergoing thymidine incorporation in 7-day autologous mixed lymphocyte reactions (AMLR). Peripheral blood mononuclear cells (PBM) were separated into B cells, T cells, B + Null cells and T + Null cell-enriched populations. Cells were cultured in various combinations. Monocytes were removed to determine their influence on AMLR. The main thymidine-incorporating cells in cultures were shown to be Null cells and to a lesser extent T cells. Monocytes were found to have a more pronounced suppressor effect on stimulation of T cells by non-T cell populations in younger individuals than in the elderly. Whether Null cells undergo a spontaneous blastogenesis in culture or are stimulated by T or B cells, could not be answered by the present investigation.
D000328:Adult; D000368:Aged; D000375:Aging; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D008215:Lymphocytes, Null / Q000276:immunology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D013936:Thymidine