The measurement of enzyme activities in endomyocardial biopsies from patients with congestive (dilated) cardiomyopathy and specific heart muscle disease.
50 patients with heart muscle disease have been investigated using routine invasive and non-invasive techniques, including endomyocardial biopsy. Enzyme activities in myocardial tissue obtained by biopsy have been measured in dilated cardiomyopathy and heart muscle disease and compared. Significant differences in enzyme activity have been found between dilated cardiomyopathy and alcoholic heart muscle disease when the groups are defined by detailed drinking histories. CPK (p less than 0.002), MDH (p less than 0.001), LDH (p less than 0.001) and alpha HBD (p less than 0.001). The changes in the enzyme levels may be an adaptive response to alcohol although serial study did not show a fall in enzyme activities after 3-6 months abstinence. Preliminary data furthermore indicates an association of the alpha HBD/LDH and LDH/CPK enzyme activity ratios and ejection fraction. This data supports the diagnosis of a specific heart muscle disease in response to alcohol excess.
D000328:Adult; D000437:Alcoholism / Q000150:complications; D001219:Aspartate Aminotransferases / Q000032:analysis; D006332:Cardiomegaly / Q000150:complications / Q000201:enzymology* / Q000473:pathology; D009202:Cardiomyopathies / Q000150:complications / Q000201:enzymology* / Q000235:genetics; D002310:Cardiomyopathy, Alcoholic / Q000201:enzymology* / Q000473:pathology; D002312:Cardiomyopathy, Hypertrophic / Q000201:enzymology / Q000473:pathology; D003402:Creatine Kinase / Q000032:analysis; D004699:Endocardium / Q000201:enzymology*; D005260:Female; D006349:Heart Valve Diseases / Q000201:enzymology / Q000473:pathology; D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology
D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase