Actin behaviour in doxorubicin treatment.
The inhibition of neural tube closure in early chick embryos explanted in vitro by New's technique was used as a model to study the possible involvement of actin polymerization during doxorubicin treatment. The investigation was performed with indirect immunofluorescence staining, fluorescence staining with NBD-Ph, and DNase I inhibition activity. 8 x 10(-6) M doxorubicin induces alteration in the distribution of actin fluorescence on neural tube and in CEF cell cytoskeleton, and an appreciable increase in the critical concentration of monomeric actin as measured in cell extracts.
D000199:Actins / Q000032:analysis*; D000818:Animals; D002457:Cell Extracts / Q000032:analysis; D002478:Cells, Cultured; D002490:Central Nervous System / Q000196:embryology* / Q000648:ultrastructure; D002642:Chick Embryo; D046508:Culture Techniques; D003599:Cytoskeleton / Q000032:analysis / Q000187:drug effects*; D003850:Deoxyribonuclease I; D004317:Doxorubicin / Q000494:pharmacology*; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique
D000199:Actins; D002457:Cell Extracts; D004317:Doxorubicin; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I