Rapid thin layer chromatographic determination of zearalenone in corn, sorghum, and wheat.
A rapid method is described for determining zearalenone in corn, sorghum, and wheat. The mycotoxin is extracted with a mixture of acetonitrile and 4% KCl in HCl. The extract is cleaned up with isooctane, evaporated, and redissolved in chloroform. Zearalenone is separated by thin layer chromatography; identity is confirmed with various developing solvents and spray reagents. Zearalenone is then quantitated by the limit detection method. The minimum detectable concentration is 140-160 micrograms/kg when aluminum chloride solution is used as spray reagent, and 85-110 micrograms/kg when Fast Violet B salt is used as spray reagent.
D002855:Chromatography, Thin Layer; D002523:Edible Grain / Q000032:analysis*; D012118:Resorcinols / Q000032:analysis*; D014908:Triticum / Q000032:analysis; D003313:Zea mays / Q000032:analysis; D015025:Zearalenone / Q000032:analysis*
D012118:Resorcinols; D015025:Zearalenone