[Total and contractile protein synthesis during experimental cardiac hypertrophy].
The fractional turnover rate of the total protein extracted from cardiac rat ventricles has been measured by the continuous infusion flow technique in unanesthesized animals using 3H-lysine as a marker. This turnover rate approximated 12 p. 100 per day, doubled after aortic stenosis or aortic incompetence, and reached a peak within a week after stenosis and 2 weeks after incompetence. The fractional turnover rate of actin and myosin heavy-chains has been measured by the same technique using 14C-tyrosine as a marker and preparative gel electrophoresis to isolate the protein. This turnover rate approximated 22 and 9 p. 100 per day for myosin and actin, respectively, and doubled 4 days after aortic stenosis. It is suggested that a drop in contraction efficiency triggered protein synthesis in cardiac overloading.
D000818:Animals; D001022:Aortic Valve Insufficiency / Q000378:metabolism; D001024:Aortic Valve Stenosis / Q000378:metabolism; D006332:Cardiomegaly / Q000378:metabolism*; D003285:Contractile Proteins / Q000096:biosynthesis*; D006352:Heart Ventricles / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003285:Contractile Proteins
Etude de la synthèse des protéines totales et des protéines contractiles dans l'hypertrophie cardiaque expérimentale.