Toxicity of oxygen radicals in cultured pulmonary endothelial cells.
Superoxide dismutase and catalase, which catalytically remove O-2 and H2O2, respectively, each separately protected cultured pulmonary artery endothelial cells from loss of membrane integrity after exposure to oxygen radicals generated either cellularly (polymorphonuclear leukocytes) or chemically (dihydroxyfumarate). Nicotinamide, a precursor of nicotinamide-adenine dinucleotide (NAD) and an inhibitor of ADP-ribose synthetase, also protected cultured endothelial cells from loss of membrane integrity in a concentration-dependent manner after exposure to DHF.
D000818:Animals; D002374:Catalase / Q000502:physiology*; D002417:Cattle; D004347:Drug Interactions; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005650:Fumarates / Q000633:toxicity*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008171:Lung Diseases / Q000139:chemically induced*; D009536:Niacinamide / Q000494:pharmacology; D011651:Pulmonary Artery / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000502:physiology*
D005650:Fumarates; C005998:dihydroxyfumarate; D009536:Niacinamide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase