Isolation and antigen analysis of surface tegument membranes from schistosomula of Schistosoma mansoni.
The outer tegument membranes of Schistosoma mansoni schistosomula have been removed by mechanical disruption in a hypotonic salt solution and partially purified by differential and sucrose density gradient centrifugation. Fractionation was monitored by measuring increase in specific activity of bound [125I]wheat-germ agglutinin (WGA), alkaline phosphatase and calcium-stimulated ATPase. Two-dimensional IEF/SDS polyacrylamide gel electrophoresis was used to analyse the peptide composition of the isolated membranes and to compare and contrast with lactoperoxidase/glucose oxidase surface labelled peptides. At least 35 surface-labelled peptides were resolved on the two-dimensional maps: all were also present in the membrane material recovered from the sucrose gradient. Western blot analysis demonstrated a marked heterogeneity in the antibody response of infected human patients to individual membrane antigens. The antigenic profile of membranes isolated from cercariae, 18 and 96 h schistosomula were compared using Western blots: some minor differences were observed between the three preparations.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D002499:Centrifugation, Density Gradient; D007457:Iodine Radioisotopes; D037102:Lectins / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D012550:Schistosoma mansoni / Q000276:immunology*; D014909:Wheat Germ Agglutinins
D000954:Antigens, Surface; D007457:Iodine Radioisotopes; D037102:Lectins; D008565:Membrane Proteins; D014909:Wheat Germ Agglutinins; D000251:Adenosine Triphosphatases