Monoclonal antibodies against subunits of yeast mitochondrial H+-ATPase.
Fourteen stable lines of myeloma-spleen cell hybrids producing antibodies against the mitochondrial H+-ATPase have been isolated. One reacted with the alpha-subunit of the enzyme complex (Mr 56000), nine with the beta-subunit (Mr 54000), and four with a 25 kDa subunit which has not been previously characterized. These antibodies are inhibitory or stimulatory or have no effect upon the enzyme activity. Two of the monoclonal anti-beta-subunit antibodies were found to be particularly effective in immunoprecipitating intact H+-ATPase complex.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D010954:Plasmacytoma / Q000276:immunology; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D046911:Macromolecular Substances; D006180:Proton-Translocating ATPases