title: A comparison of the in vitro cytotoxicity of four restorative materials assessed by changes in enzyme levels in two cell types.
abstract: A system is described for assessing the toxicity of freshly mixed restorative materials in vitro by measuring changes in the levels of beta-galactosidase and lactic dehydrogenase in both cultured cells and supernatants. The toxic effects of a zinc phosphate and silicate cement, a composite, and zinc oxide/eugenol were studied on two cell types, macrophages and fibroblasts, after 24 h exposure. Zinc oxide/eugenol, Silicap, and zinc phosphate were toxic to macrophages, in that order; Concise appeared to be nontoxic. Only zinc oxide/eugenol exerted significant effects on fibroblasts. Interposing dentine powder between the test cells and the material ameliorated the effects of all materials, possibly by the absorption of toxic components.
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate; D002478:Cells, Cultured; D003764:Dental Materials / Q000633:toxicity*; D003793:Dental Restoration, Permanent; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005696:Galactosidases / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D012823:Silicate Cement / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity; D015036:Zinc Phosphate Cement / Q000633:toxicity; D001616:beta-Galactosidase / Q000032:analysis*
chemical_list: D000180:Acrylic Resins; D003764:Dental Materials; D015035:Zinc Oxide-Eugenol Cement; D012823:Silicate Cement; D017438:Bisphenol A-Glycidyl Methacrylate; D015036:Zinc Phosphate Cement; D007770:L-Lactate Dehydrogenase; D005696:Galactosidases; D001616:beta-Galactosidase