Polyacrylamide microbeads with entrapped protein A from S. aureus for cell separation.
A technique was developed for the identification and separation of cells according to their surface antigenic markers, based on immobilization of protein A by entrapment in polyacrylamide microbeads. This method is further development of the previous techniques with SpA-containing S. aureus bacteria, but has the advantage of having less unspecific binding to the cells. We show that this technique might be a useful tool for the separation of cells, exemplified by separation of cells on Petri dishes coated with a monolayer of SpA-microbeads. The viability of separated cells was unaffected and adherent cells were easily removed by trypsin.
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008863:Microspheres; D011817:Rabbits; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism; D000099166:Polyacrylamides
D000180:Acrylic Resins; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D013205:Staphylococcal Protein A; D000099166:Polyacrylamides