The relationship between the vitro activity of 3 beta-hydroxysteroid dehydrogenase delta 4-5-isomerase in human sebaceous glands and their secretory activity in vivo.
3 beta-hydroxysteroid dehydrogenase delta 4-5-isomerase (delta 5-3 beta-HSD) catalyzes an early step in the synthesis of testosterone from dehydroepiandrosterone (DHA). We compared enzyme activity in back skin biopsies with sebum excretion rate (SER) in 14 individuals. The rate of conversion of [7 alpha-3H]DHA into [3H]-4-androstene-3,17-dione was measured in cryostat sections of skin and compared with the sebaceous gland content of the same biopsies. Reaction rate was proportional to the volume of sebaceous gland tissue in the sections. Enzyme activity was absent from sections without histologically identifiable sebaceous gland tissue. This suggests that the delta 5-3 beta-HSD is localized in sebaceous glands. SER, measured by a modified photometric technique at the biopsy site, correlated highly with sebaceous gland volume and with the rate of conversion of DHA into androstenedione in the biopsy. For each biopsy, specific activity of delta 5-3 beta-HSD in sebaceous glands was calculated by dividing the rate of formation of [3H]-4-androstene-3,17-dione by sebaceous gland volume. Specific activity of delta 5-3 beta-HSD did not correlate significantly with SER, suggesting that variations in concentration of delta 5-3 beta-HSD in sebaceous glands probably do not underlie variations in sebaceous gland activity.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000735:Androstenedione / Q000378:metabolism; D001706:Biopsy; D003687:Dehydroepiandrosterone / Q000378:metabolism; D005260:Female; D006801:Humans; D007535:Isomerases / Q000378:metabolism*; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism*; D011375:Progesterone Reductase; D012627:Sebaceous Glands / Q000201:enzymology* / Q000378:metabolism; D012629:Sebum / Q000378:metabolism; D013251:Steroid Isomerases / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis
C021425:3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase; D009097:Multienzyme Complexes; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D015096:3-Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase; D007535:Isomerases; D013251:Steroid Isomerases