Isoenzyme patterns of pathogenic and non-pathogenic Naegleria spp. using agarose isoelectric focusing.
Using agarose isoelectric focusing, the isoenzyme patterns of 7 different enzymes were compared in 52 Naegleria strains. The pathogenic N. fowleri was found the most homogeneous species. N. lovaniensis seems to be constituted of different types which form nevertheless a cohesive group. Within N. gruberi, large interstrain band variations were found in almost all enzyme systems. A re-examination of the taxonomic position of this species may therefore be taken into consideration. High temperature strains from Australia were confirmed to be different from N. lovaniensis. Members of a new pathogenic Naegleria sp., N. australiensis, seem to occur in Europe. Large thermophilic strains with many large pores in the cysts show identical zymograms and may constitute a new species or genus.
D000135:Acid Phosphatase / Q000032:analysis; D000656:Amoeba / Q000145:classification / Q000201:enzymology*; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005969:Glutamate Dehydrogenase / Q000032:analysis; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D010733:Phosphoglucomutase / Q000032:analysis
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D002265:Carboxylic Ester Hydrolases; C034216:propionyl esterase; D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase