ATPase activity and ATP-dependent proton translocation in plasma membrane vesicles of turtle bladder epithelial cells.
ATP-induced quenching of fluorescence of acridine orange (a pH probe) or Oxonol V (a potential difference probe) is evoked in turtle bladder membrane vesicles in suspending media of appropriate ionic composition and is insensitive to oligomycin, valinomycin, and ouabain. These effects are ascribed to a membrane-bound, ouabain-resistant ATPase which mediates an active electrogenic proton transport.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D005453:Fluorescence; D010042:Ouabain / Q000494:pharmacology; D006180:Proton-Translocating ATPases; D014426:Turtles; D001743:Urinary Bladder / Q000187:drug effects / Q000201:enzymology*
D010042:Ouabain; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases