In vitro life span and "unscheduled DNA synthesis" in subconfluent cultures and clones of trisomic and normal diploid fibroblasts.
"Unscheduled DNA synthesis" as measured autoradiographically after exposure to ultraviolet light irradiation was determined in subconfluent fibroblast culture in different clones from two patients with Down's syndrome and in normal diploid human fibroblasts. The trisomic fibroblast cultures exhibited a marked reduction and clonal heterogeneity in repair capacities as well as reduced in vitro life spans when compared to the diploid control cells.
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D002999:Clone Cells / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis* / Q000528:radiation effects; D004260:DNA Repair; D004314:Down Syndrome / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D014466:Ultraviolet Rays
D004247:DNA