Functional and chemical characterization of B-cell growth factor produced by normal cloned T helper cells.
Media conditioned by clones of normal helper T cells exposed to appropriate antigen-presenting cells contain growth-promoting activity for B-cell blasts induced either by lipopolysaccharide or on direct interaction with competent helper cells. This B-cell growth factor (TH-BGAPet) is recovered on sodium doecyl sulphate polyacrylamide gel electrophoresis corresponding to mol. wt of 15,000-20,000 displays no mitogenicity for small, non-induced B lymphocytes and is completely devoid of the ability to activate immunoglobulin secretion in proliferating B cells. These results are ascribed to the activity derived from normal T cells, with the same characteristics as BSF-p1 previously obtained from lymphomas and hybridomas. Since hybridization of these T helper cells results in the constitutive production of BSF-p1 in the absence of macrophages, these experiments demonstrate that BSF-p1 is a normal T-cell product.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002999:Clone Cells; D003470:Culture Media; D006133:Growth Substances; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines; D051379:Mice; D008970:Molecular Weight; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D003470:Culture Media; D006133:Growth Substances; D008222:Lymphokines; D015847:Interleukin-4