Serological identification and separation of canine lymphocyte subpopulations.
In vitro treatment of bone marrow grafts with absorbed rabbit-antidog thymocyte globulin (ATG) prevented graft-versus-host disease in a substantial number of the dogs differing by one DLA haplotype. Absorbed ATG has now been used for serological identification of canine lymphocyte populations. Specific labeling of canine T-lymphocytes by absorbed ATG could be demonstrated by (a) a distribution of ATG-positive cells in suspensions of canine lymphoid organs similar to that of T cells observed in other species and by specific staining of paracortical thymus-dependent lymph node areas in immunohistochemistry, (b) complementary labeling of nylon-wool-separated cells by ATG and antiimmunoglobulin sera, and (c) correlation of ATG surface labeling with responder activities in mixed lymphocyte cultures.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology*; D001854:Bone Marrow Cells; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D004285:Dogs; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D008198:Lymph Nodes / Q000166:cytology; D007959:Lymphocyte Culture Test, Mixed; D008938:Mitosis; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000961:Antilymphocyte Serum; D000939:Epitopes; D010835:Phytohemagglutinins; D003208:Concanavalin A