Difference infrared spectroscopy of aqueous model and biological membranes using an infrared data station.
A Perkin-Elmer infrared Data Station associated with a simple IR spectrometer (model 298) is shown to give excellent results with aqueous model and biomembrane systems. Examples are presented of difference spectra obtained with lipid--water systems, reconstituted lipid--protein systems and a natural biomembrane. The spectra of the lipid after water subtraction and of the intrinsic protein after lipid subtraction from a model reconstituted Ca2+-ATPase membrane system are shown. The potential for studying intrinsic protein conformations is emphasised.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis; D007425:Intracellular Membranes / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008566:Membranes / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000032:analysis; D013055:Spectrophotometry, Infrared / Q000379:methods*; D014867:Water
D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D014867:Water; D000252:Calcium-Transporting ATPases