Complement activation by synthetic vascular prostheses.
Morphologic evaluation of synthetic grafts (both seeded and unseeded) harvested within 2 weeks of implantation has revealed heavy infiltration with polymorphonuclear leukocytes (PMNs). Since complement-activated PMNs are known to damage endothelial cells, we hypothesized that complement activation might prove a barrier to optimal endothelial cell seeding of prosthetic grafts. To determine whether synthetic vascular prostheses activate complement and whether the type of graft material influences the degree of activation, we assayed the plasma of 10 healthy donors for complement activity following incubation with short segments of knitted Dacron and polytetrafluoroethylene (PTFE) graft material. C5a generation was measured by radioimmunoassay and granulocyte aggregometry. Standard hemolytic assays were used to determine depletion of functional classical pathway (CH50 and C4) alternative pathway (APH50) activity. Results indicated substantial complement activation by Dacron and virtually none by PTFE. Activation by Dacron appeared to occur via both complement pathways. Such complement "reactivity" may have important implications for the performance of prosthetic materials as small-caliber vascular grafts, whether seeded with endothelial cells or not.
D001807:Blood Vessel Prosthesis / Q000009:adverse effects / Q000379:methods; D002478:Cells, Cultured; D003167:Complement Activation; D003181:Complement C4 / Q000032:analysis; D003182:Complement C5 / Q000032:analysis; D015936:Complement C5a; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D004727:Endothelium / Q000166:cytology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000276:immunology; D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene; D011863:Radioimmunoassay
D003181:Complement C4; D003182:Complement C5; D011093:Polyethylene Terephthalates; D015936:Complement C5a; D011138:Polytetrafluoroethylene