Primary T-cell responses to minor alloantigens. II. Analysis of accessory cell requirements for the development of cytotoxic T lymphocytes.
The accessory cell requirements of cytotoxic T-lymphocyte (CTL) responses directed at multiple minor alloantigens are examined using a short-term, combined in-vivo and in-vitro protocol. The development of cytotoxic activity in vitro from T cells sensitized in vivo requires low-density accessory cells derived from the immunizing strain which have to be H-2 compatible with the responder population. The accessory activity of these cells can be by-passed by interleukin-2 (IL-2)-containing supernatant. Since IL-2 is a product of helper T (Th) cells secreted upon activation by antigen recognized in context of self H-2 and is known to stimulate antigen-activated cytotoxic T-lymphocyte precursors (CTL-P) non-specifically to effector CTL function, the results indicate that accessory cells interact in an H-2 restricted fashion with helper rather than cytotoxic T-lymphocyte precursors. The low-density accessory cells active in this system do not express Fc receptors (FcR) and are present in both the adherent and non-adherent fractions of spleen or lymph nodes.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D007519:Isoantigens / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011961:Receptors, Fc / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D006183:H-2 Antigens; D007376:Interleukin-2; D007519:Isoantigens; D011961:Receptors, Fc