Complexing of glycolipids and their transfer between membranes by the activator protein for degradation of lysosomal ganglioside GM2.
The lysosomal degradation of ganglioside GM2 by hexosaminidase A depends on the presence of the specific activator protein which mediates the interaction between micellar or membrane-bound ganglioside and water-soluble hydrolase. The mechanism and the glycolipid specificity of this activator were studied in more detail. 1. It could be shown with three different techniques (isoelectric focusing, centrifugation and electrophoresis) that the activator protein extracts glycolipid monomers from micelles or liposomes to give water-soluble complexes with a stoichiometry of 1 mol of glycolipid/mol of activator protein. Liposome-bound ganglioside GM2 is considerably more stable against extraction and degradation than micellar ganglioside. 2. In the absence of enzyme the activator acts in vitro as glycolipid transfer protein, transporting glycolipids from donor to acceptor membranes. 3. The activator protein is rather specific for ganglioside GM2. Other glycolipids (GM3 GM1, GD1a and GA2) form less stable complexes with the activator and are transferred at a slower rate (except for ganglioside GM1) than ganglioside GM2.
D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005678:G(M2) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism; D006023:Glycoproteins; D054818:Hexosaminidase A; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D008081:Liposomes; D008247:Lysosomes / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism*; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D014461:Ultracentrifugation; D001619:beta-N-Acetylhexosaminidases
D005732:Gangliosides; D006017:Glycolipids; D006023:Glycoproteins; D008081:Liposomes; C483345:PSAP protein, human; D011506:Proteins; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D005678:G(M2) Ganglioside; D006596:Hexosaminidases; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases