A study of the heat-shock response in Neurospora crassa.
1. Neurospora crassa was grown at 28 degrees C for 12 hr and transferred to higher temperatures for 2 hr. 2. Cultures labelled with [35S]methionine showed the synthesis of several new proteins in response to heat-shock at 46 to 48 degrees C. 3. Major polypeptides of approximate Mr 105,000, 99,000, 78,000, 43,000 and 23,000 were detectable in one-dimensional SDS-polyacrylamide slab gel electropherograms. 4. 2-D analysis using isoelectric-focussing in the first dimension and electrophoresis in SDS-polyacrylamide gels in the second led to the resolution of some of the heat-induced polypeptide into multiple spots differing in pI values. 5. mRNA from heat-shocked cells was translated poorly in Wheat Germ extract and rabbit reticulocyte lysate in vitro translation systems.
D000222:Adaptation, Physiological; D005656:Fungal Proteins / Q000096:biosynthesis; D006358:Hot Temperature; D009491:Neurospora / Q000502:physiology*; D009492:Neurospora crassa / Q000502:physiology*; D010455:Peptides / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D005656:Fungal Proteins; D010455:Peptides; D012331:RNA, Fungal; D012333:RNA, Messenger