[Functional properties of a lymphocyte subpopulation responding to low suboptimal doses of concanavalin A].
Incubation of human peripheral blood lymphocytes with concanavalin A (Con A), in a low suboptimal dose (0.5 microgram/ml), results in formation of the cells that inhibit proliferation of autologous cells in cultures activated with optimal but not with suboptimal dose of the mitogen. Nevertheless, 50 micrograms/ml Con A-activated cells efficiently suppress proliferation everywhere. Cell preincubation during 18 h before Con A activation leads to a reduction of lymphocyte responses to the mitogen in cultures reactivated with 5 micrograms/ml Con A in a mixture with autologous lymphocytes, containing no mitogen. Activation of T-T helper cells providing suppressor T cells differentiation seems to take place in the presence of a low suboptimal dose of Con A. Besides, 0.5 microgram/ml Con A prevents the preincubation-induced elimination of some lymphocytes responding to an optimal dose of Con A and autologous lymphocytes.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects
D003208:Concanavalin A
Funktsional'nye svoĭstva subpopuliatsii limfotsitov, otvechaiushchikh na nizkie suboptimal'nye dozy konkanavalina A.