Stimulation by serotonin of 40 kDa and 20 kDa protein phosphorylation in human platelets.
In human platelets, serotonin is known to induce a shape change followed by (reversible) aggregation. Recently, it was found that the amine triggers the elevation of cytosolic free calcium and activates phospholipase C. On stimulation of human platelets with serotonin we found an immediate increase in protein kinase C activity, phosphorylating its 40 kDa substrate protein. A 20 kDa protein, most likely the myosin light chain, was phosphorylated to the same extent. Ketanserin, a highly selective serotonin-S2 antagonist inhibited both phosphorylation processes at subnanomolar concentrations.
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D007650:Ketanserin; D008970:Molecular Weight; D009219:Myosin-Light-Chain Kinase; D010712:Phosphatidic Acids / Q000097:blood; D010766:Phosphorylation; D010880:Piperidines / Q000494:pharmacology; D011493:Protein Kinase C; D011494:Protein Kinases / Q000097:blood; D011985:Receptors, Serotonin / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology*
D001798:Blood Proteins; D010712:Phosphatidic Acids; D010880:Piperidines; D011985:Receptors, Serotonin; D012701:Serotonin; D007650:Ketanserin; D011494:Protein Kinases; D011493:Protein Kinase C; D009219:Myosin-Light-Chain Kinase