The semi-quantitative distribution of T4 and T6 surface antigens on human Langerhans cells.
We have used indirect immunogold electron microscopy to compare the respective density of cell membrane determinants revealed by OKT6 and OKT4 monoclonal antibodies on normal human Langerhans cells (LC): 12.9 +/- 3.5 gold granules were noted per cell section on OKT4-positive LC whereas 236.8 +/- 23.5 granules were counted per cell section on OKT6-reactive cells. These results confirm that human LC react with OKT4 antibody and they demonstrate a marked quantitative difference on LC surface between the antigenic determinants recognized by OKT6 and OKT4 antibodies.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D000939:Epitopes / Q000032:analysis; D006046:Gold; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D006046:Gold