The noninvasive biochemical diagnosis of gastrointestinal disease, with special reference to children.
Invasive tests to diagnose patients with gastrointestinal disease are rapidly being replaced by procedures which enable organ function to be assessed by monitoring the product of a metabolic reaction in readily available materials such as breath, blood, and urine. Examples of these approaches that will be assessed in this review include the hydrogen breath test for lactase deficiency, radioactive carbon dioxide breath measurements to test for fat digestion and absorption, and tests of pancreatic function based upon synthetic substrates from which fluorescein or para-aminobenzoic acid can be liberated by pancreas-specific enzymes. Significant advances have been made in improving the organ sensitivity of enzyme determinations. The determination of amylase isoenzymes has been less useful than the measurement of immunoreactive trypsin; this latter enzyme is greatly elevated in the blood of neonates with cystic fibrosis, whereas serum levels are greatly depressed in cystic fibrosis patients with pancreatic insufficiency as well as in most patients with steatorrhea due to chronic pancreatitis. Many of these tests are now becoming standard procedures in the investigation of infants with gastrointestinal disease.
D000681:Amylases / Q000032:analysis; D001647:Bile Acids and Salts / Q000032:analysis; D001944:Breath Tests; D002241:Carbohydrates / Q000032:analysis; D002248:Carbon Monoxide / Q000032:analysis; D002250:Carbon Radioisotopes; D002648:Child; D002675:Child, Preschool; D005227:Fatty Acids / Q000032:analysis; D005767:Gastrointestinal Diseases / Q000175:diagnosis*; D006801:Humans; D006859:Hydrogen / Q000032:analysis; D007223:Infant; D007231:Infant, Newborn; D010186:Pancreatic Function Tests; D014357:Trypsin / Q000032:analysis
D001647:Bile Acids and Salts; D002241:Carbohydrates; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D002248:Carbon Monoxide; D006859:Hydrogen; D000681:Amylases; D014357:Trypsin