Recovery from impairment in feeding response to glucoprivic stimuli and in sensitivity to diabetogenic agents in riboflavin-repleted rats.
Male weanling rats, fed a riboflavin-deficient diet for 14 days showed impairments in reactivity to the hyperphagic action of either insulin or 2-deoxy-D-glucose (2DG) and in sensitivity to the diabetogenic action of streptozotocin or alloxan. The intraperitoneal injection of riboflavin (160 micrograms/rat) resulted in an immediate restoration in FAD-dependent activation of erythrocyte glutathione reductase and in the reactivity of food intake to insulin, whereas the reactivity of food intake to 2DG was restored after 3 days of riboflavin repletion. The sensitivity to diabetogenic agents was not restored solely by the riboflavin injection but required 3 hours of feeding as well. These findings indicate that the riboflavin deficiency caused some defects at specific glucosensitive sites localized in the pancreas and the brain and that some metabolic processes were necessary to restore the sensitivity.
D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology; D005247:Feeding Behavior / Q000187:drug effects*; D005980:Glutathione Reductase / Q000097:blood; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012256:Riboflavin / Q000008:administration & dosage*; D012257:Riboflavin Deficiency / Q000097:blood; D013311:Streptozocin / Q000494:pharmacology*; D013997:Time Factors
D001786:Blood Glucose; D003837:Deoxy Sugars; D007328:Insulin; D013311:Streptozocin; D000496:Alloxan; D003847:Deoxyglucose; D005980:Glutathione Reductase; D012256:Riboflavin