title: Sequencing of large double-stranded DNA using the dideoxy sequencing technique.
abstract: The dideoxy sequencing technique has been applied to the direct sequencing of large double-stranded DNA molecules with a small single-stranded primer. For instance, the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure proves useful in the sequence analysis of a large number of different mutations in a particular region and in the analysis of eukaryotic DNA cloned in plasmids, phages, and cosmids.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004247:DNA; D004279:DNA, Viral; D005814:Genes, Viral; D008722:Methods; D008970:Molecular Weight; D010761:Phosphorus Radioisotopes
chemical_list: D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D004247:DNA