EDTA separation and ATPase Langerhans cell staining in the mouse epidermis.
Sheets of ethylenediaminetetraacetic acid (EDTA)-separated epidermis were examined using scanning electron, transmission electron, and light microscopy; sheets were also examined after staining for adenosine triphosphatase (ATPase) activity. Staining was improved by longer incubation with EDTA and by elimination of Trismal buffer as a tissue rinse. EDTA-separated epidermis showed better retention of ultrastructural integrity when washed with phosphate-buffered saline. The ATPase staining procedures described in this present study are ultrastructurally specific for the Langerhans cell.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D006651:Histocytochemistry; D007801:Langerhans Cells / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
D000251:Adenosine Triphosphatases