[Purification of alpha-1,4 leads to 1,4-glucosyltransferase from horse blood serum].
The purification of alpha-1,4-1,4-glucosyltransferase from the equine serum is presented. Ion-exchange chromatography on DE-11, DE-32 and CM-32 celluloses was applied in the successive steps of isolation. Gel-filtration on Bio-Gel P-200 was the last step of purification; it gave the protein which was homogeneous on disc polyacrylamide gel electrophoresis. The purification degree was of the order 2100 at about 40% yield.
D015061:1,4-alpha-Glucan Branching Enzyme / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D005964:Glucosyltransferases / Q000097:blood*; D006736:Horses / Q000097:blood*
D005964:Glucosyltransferases; D015061:1,4-alpha-Glucan Branching Enzyme
Oczyszczanie alfa-1,4 leads to 1,4-glukozylotransferazy z surowicy krwi konia.