Effect of seven inhibitors on invertases in homogenates of human dental plaque.
Seven known alpha-glucosidase/beta-fructosidase inhibitors were examined for inhibitory effect against invertases in pooled human dental plaque. The inhibitors used were acarbose, Trestatin, nojirimycin, 1-deoxynojirimycin, glucono-delta-lactam, conduritol-B-epoxide, and Hoe 467A. Plaque homogenates were incubated with 14C-labelled sucrose and invertase activity was assayed by determination of radio-labelled monosaccharide after paper chromatography. Conduritol-B-epoxide, acarbose, and Trestatin reduced the invertase activity an average of 62%, 51%, and 35% respectively. The other inhibitors affected the enzyme activity negligibly. By combining conduritol-B-epoxide with acarbose or Trestatin in other plaque homogenates the inhibition of invertases increased from 35% to 61%. This observation supported the concept that the invertases in dental plaque consist of both alpha-glucosidases and beta-fructosidases.
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives; D020909:Acarbose; D003773:Dental Plaque / Q000201:enzymology*; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005959:Glucosidases / Q000037:antagonists & inhibitors*; D065089:Glycoside Hydrolase Inhibitors; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D006801:Humans; D007294:Inositol / Q000031:analogs & derivatives / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D010881:Piperidones / Q000494:pharmacology; D014312:Trisaccharides / Q000494:pharmacology; D043324:beta-Fructofuranosidase
D065089:Glycoside Hydrolase Inhibitors; D010455:Peptides; D010881:Piperidones; D014312:Trisaccharides; C038504:5-amino-5-deoxygluconic acid delta-lactam; D017485:1-Deoxynojirimycin; D007294:Inositol; C038505:trestatin; D005959:Glucosidases; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005944:Glucosamine; C011423:conduritol epoxide; D020909:Acarbose; C013626:nojirimycin; C030526:tendamistate