The toxicity of fluorodeoxyuridine when used to increase the uptake of 125I-iododeoxyuridine into tissue culture cells in vitro.
The use of fluorodeoxyuridine (FUdR) to increase the uptake of 125I-iododeoxyuridine (125IUdR) into tissue culture cells was investigated. FUdR was found to be chemically toxic in itself to 2 canine tissue culture lines at doses used in the literature to increase 125IUdR uptake. FUdR had a cytocidal effect on one cell line, but a cytostatic effect on the other, so that measurement of only cell viability or death in the latter would not have shown a toxic effect. FUdR was shown to mask the radiotoxic effects of 125IUdR and so its use in investigations of the toxicity of 125IUdR may have produced misleading results.
D000818:Animals; D002460:Cell Line; D002477:Cells / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D004285:Dogs; D005467:Floxuridine / Q000633:toxicity*; D007065:Idoxuridine / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism*; D008545:Melanoma / Q000473:pathology; D012516:Osteosarcoma / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology
D007457:Iodine Radioisotopes; D005467:Floxuridine; D007065:Idoxuridine