Photodynamic effects of hematoporphyrin-derivative on transmembrane transport systems of murine L929 fibroblasts.
Photodynamic treatment of murine L929 fibroblasts with hematoporphyrin-derivative causes deterioration of various membrane functions. Most sensitive to photodynamic inactivation are the energy-coupled transport systems for aminoisobutyric acid and for Rb+. The facilitated diffusion system for 2-deoxy-D-glucose is slightly less sensitive. After longer illumination periods also the membrane barrier function is impaired, as reflected by K+ leakage and increased passive Rb+ uptake. After still longer illumination periods intermolecular protein crosslinking can be observed. This makes it unlikely that intermolecular protein crosslinking is causally involved in the deterioration of these membrane functions.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000494:pharmacology*; D006593:Hexokinase / Q000037:antagonists & inhibitors; D007700:Kinetics; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010777:Photochemistry; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative; D006593:Hexokinase