title: T cell regulation of the IgG2a response to TNP-Ficoll: evidence that allotype congenic mice contain both helper cells that preferentially enhance IgG2a synthesis and suppressor cells that specifically suppress IgG2 synthesis.
abstract: When unprimed C57BL T cells were transferred into C57BL nu/nu mice, a preferential enhancement of the IgG2a antibody response to TNP-Ficoll was observed. Unprimed splenic T cells from Igh allotype congenic (B.C8) mice were unable to enhance the IgG2a response. The failure of T cells from allotype congenic mice to augment IgG2a antibody production to TNP-Ficoll was due to the presence of a T cell that specifically suppressed IgG2a antibody synthesis. The suppressive activity could be demonstrated in nu/nu mice and nu/nu mice reconstituted with C57BL helper cells. The suppressive activity of the B.C8 T cell population could be abrogated by treatment with anti-Lyt-1 and anti-Lyt-2 antibodies and complement as well as by treatment of B.C8 T cells with anti-Lyt-2 alone. Removal of the T cells responsible for IgG2a suppression from B.C8 splenic T cell population unmasked a population of B.C8 T cells that could enhance IgG2a preferentially augment IgG2a antibody synthesis can be found in donor mice that differ in their Igh background from the responding B cells.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000888:Antibodies, Anti-Idiotypic; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology; D002469:Cell Separation; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000950:Antigens, Ly; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D009578:Nitrobenzenes; D011134:Polysaccharides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll