Effects of some mycotoxins on mitogen-induced blastogenesis and SCE frequency in human lymphocytes.
The effects of T-2 toxin, diacetoxyscirpenol, ochratoxin A and zearalenone on DNA synthesis in phytohaemagglutinin-stimulated human peripheral blood lymphocytes were studied by assaying the incorporation of [3H]thymidine. Total inhibition was obtained with 8 ng T-2 toxin/ml, 8 ng diacetoxyscirpenol/ml or 30 micrograms zearalenone/ml, and with 20 micrograms ochratoxin A/ml inhibition was almost complete; 50% inhibition was produced by 1.5 ng T-2 toxin/ml, 2.7 ng diacetoxyscirpenol/ml, 14 micrograms zearalenone/ml or 14 micrograms ochratoxin A/ml. The toxicity of the trichothecenes to the lymphocytes was slightly reduced when rat liver cells were present whereas the toxicity of ochratoxin A and zearalenone was unaltered. Low concentrations of trichothecenes did not produce any inhibition of DNA synthesis when the cultures were washed and placed in fresh media containing only phytohaemagglutinin 4 hr after the addition of the test compounds. Sister chromatid exchange frequency was not elevated by T-2 toxin, diacetoxyscirpenol or ochratoxin A. Zearalenone had a weak enhancing effect on the frequency of sister chromatid exchanges.
D000818:Animals; D001711:Biotransformation; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D009183:Mycotoxins / Q000378:metabolism / Q000633:toxicity*; D009793:Ochratoxins / Q000633:toxicity; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013605:T-2 Toxin / Q000633:toxicity; D014255:Trichothecenes / Q000633:toxicity; D015025:Zearalenone / Q000633:toxicity
D009183:Mycotoxins; D009793:Ochratoxins; D010835:Phytohemagglutinins; D014255:Trichothecenes; C025589:ochratoxin A; D015025:Zearalenone; D004247:DNA; D013605:T-2 Toxin; C001809:diacetoxyscirpenol