Isolation and characterization of transverse tubule from normal and dystrophic mice.
I have recently reported the isolation and characterization of sarcoplasmic reticulum from normal and dystrophic mice. These sarcoplasmic reticulum fractions were similar in calcium pump function, calcium release properties, and lipid composition. In this report, I describe the isolation of mouse muscle transverse tubule membranes using a calcium phosphate-loading technique. When the relative purity of normal and dystrophic preparations was considered, transverse tubule from normal and dystrophic mice were similar in calcium-insensitive ATPase activity, cholesterol content, and membrane microviscosity (as estimated by fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene); transverse tubule yield from dystrophic muscle, however, was twice that from normal muscle, while sarcoplasmic reticulum yield from these same dystrophic muscles was only 60% that from normal muscle. This result may reflect a difference in the relative quantities of these membranes in situ.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium; D002458:Cell Fractionation / Q000379:methods; D005614:Freeze Fracturing; D008563:Membrane Lipids / Q000032:analysis; D051379:Mice; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009137:Muscular Dystrophy, Animal / Q000473:pathology*; D012508:Sarcolemma / Q000201:enzymology / Q000648:ultrastructure*
D008563:Membrane Lipids; D000251:Adenosine Triphosphatases; D002118:Calcium