Increase of basal cell membrane area of the retinal pigment epithelium in experimental diabetes.
Stereological analysis of electron micrographs of the pigment epithelium of rats with drug-induced diabetes demonstrated an increase of plasma membrane surface area at the basal aspect of the cells. In none of the diabetic animals examined was there any evidence of breakdown of the blood-retinal barrier to the protein tracer, horseradish peroxidase. Statistically significant increases in basal plasma membrane length and surface density (surface area per unit cell volume) were measured in both streptozotocin and alloxan-injected rats after four weeks of diabetes. When hyperglycemia in streptozotocin-injected rats was promptly reversed by transplantation of normal pancreatic islets, the increase of membrane surface area did not occur. We conclude, therefore, that increased basal surface area of pigment epithelial cells is related to the diabetic condition rather than to a toxic action of the diabetogenic agents. Furthermore, increased membrane surface area was present in streptozotocin-diabetic rats killed after six months of diabetes indicating that the structural change is relatively stable. Relation of basal membrane alteration in the pigment epithelium to any functional disturbance of the barrier cell layer or of the retina in diabetes remains to be established.
D000496:Alloxan; D000818:Animals; D001692:Biological Transport; D001769:Blood; D002462:Cell Membrane / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D006735:Horseradish Peroxidase / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008297:Male; D010857:Pigment Epithelium of Eye / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin; D013997:Time Factors
D013311:Streptozocin; D000496:Alloxan; D006735:Horseradish Peroxidase