Conservative integration of bacteriophage Mu DNA into pBR322 plasmid.
In order to clarify the first step in Mu integrative recombination, we have infected a bacterial strain harboring the plasmid pBR322 and isolated Mu DNA in a supercoiled form associated with this plasmid. These structures show an association of Mu with PBR322 without any preliminary replication.
D010583:Bacteriophage mu / Q000235:genetics*; D002500:Centrifugation, Isopycnic; D004274:DNA, Recombinant / Q000032:analysis; D004278:DNA, Superhelical / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D008242:Lysogeny; D010957:Plasmids; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D004278:DNA, Superhelical; D004279:DNA, Viral