Cytotoxicity and mutagenicity of cis platin assayed in neurospora.
A Neurospora spheroplast assay was used to study the cytotoxicity and mutagenicity of cis platin (cis dichlorodiammine platinum II) in a eukaryote. Mutagenicity was measured by using reversion of several alleles of the am (NH3 assimilatory glutamate dehydrogenase) gene. A mutation (uvs-2) that disables a DNA repair pathway in Neurospora resulted in reduced sensitivity to and elimination of mutability by cis platin. The implications for other eukaryotic systems (including human cells) are discussed.
D000483:Alleles; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009491:Neurospora / Q000187:drug effects*; D009492:Neurospora crassa / Q000187:drug effects* / Q000235:genetics
D002945:Cisplatin