A cytotoxic mononuclear phagocytic cell line.
A human mononuclear phagocytic cell line (M22) was established from lymph node cells of a cancer patient. Little or no multiplication occurred in the absence of target cells such as primary or continuous cultures of human, monkey, or mouse. In response to a target stimulus, M22 altered from the resting thick-walled stage and in time, target cells were destroyed. M22 resembled a monocyte, possessing Fc and complement receptors, strong nonspecific esterase activity, pinocytosis, and lacking B- and T-cell markers but differed by lacking lysozyme. It possessed acid and alkaline phosphatases but lacked myeloperoxidase. Unlike other described monocyte lines, M22 was unique in its nonspecific cytotoxic activity.
D000328:Adult; D000818:Animals; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002143:Callitrichinae; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007231:Infant, Newborn; D008198:Lymph Nodes / Q000473:pathology; D016177:Macrophage-1 Antigen; D051379:Mice; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D010587:Phagocytosis; D011951:Receptors, Complement / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D017452:Receptors, IgG
D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D011961:Receptors, Fc; D017452:Receptors, IgG