Atrial natriuretic factor in rat hypothalamus, atria and plasma: determination by specific radioimmunoassay.
A rapid and reproducible radioimmunoassay method was developed for rat atrial natriuretic factor (ANF)-IV. The method is also applicable to human atrial peptide. ANF was detected in rat hypothalamus (5.03 pmoles/g tissue), right (86.8 pmoles/mg tissue) and left atria (52.5 pmoles/mg tissue), and plasma (156 fmoles/ml). After high salt intake immunoreactive ANF in atria and plasma increased significantly, while a significant decrease was observed in hypothalamus. Gel chromatography revealed high and low molecular weight ANF in atria and hypothalamus while only a low molecular weight form was found in plasma.
D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex; D009320:Atrial Natriuretic Factor; D001798:Blood Proteins; D002850:Chromatography, Gel; D006325:Heart Atria / Q000032:analysis; D006801:Humans; D007031:Hypothalamus / Q000032:analysis*; D007106:Immune Sera; D008297:Male; D009124:Muscle Proteins / Q000032:analysis* / Q000097:blood; D009206:Myocardium / Q000032:analysis*; D009318:Natriuresis; D045283:Natriuretic Agents; D009928:Organ Specificity; D011506:Proteins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D000936:Antigen-Antibody Complex; D001798:Blood Proteins; D007106:Immune Sera; D009124:Muscle Proteins; D045283:Natriuretic Agents; D011506:Proteins; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor