Use of the airfuge for analysis and preparation of receptors incorporated into liposomes: studies with the receptor for immunoglobulin E.
A Beckman Airfuge has been employed for studying the interaction between lipids and the receptor for immunoglobulin E (IgE). For analytic experiments, samples were applied underneath a discontinuous sucrose gradient. After a 30-min centrifugation in a fixed-angle rotor, liposomes floated toward the top of the gradient whereas unincorporated receptor-IgE complexes remained at the bottom of the tube. Liposomes with incorporated receptors were also efficiently separated in the ACR-90 preparative rotor. These methods of "Airfuge flotation" can provide useful adjuncts to more traditional methods for density-gradient centrifugation especially when rapid analysis of small samples is desired.
D000818:Animals; D002499:Centrifugation, Density Gradient; D008055:Lipids / Q000032:analysis*; D008081:Liposomes / Q000032:analysis*; D051381:Rats; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000302:isolation & purification*
D008055:Lipids; D008081:Liposomes; D017455:Receptors, IgE; D011971:Receptors, Immunologic