The proton-translocating ATPase of Candida tropicalis plasma membrane.
Proton translocation activity of Candida tropicalis plasma membrane ATPase has been demonstrated using a fluorescent delta pH probe (ACMA) and by direct pH measurements. Modifications in fluorescence intensity and H+ transport are highly specific for Mg2+ and ATP, and are sensitive to the well-known inhibitors of the plasma membrane ATPase, vanadate and DCCD. A H+/ATP ratio of 0.54 is found.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000609:Aminoacridines; D002175:Candida / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D006180:Proton-Translocating ATPases
D000609:Aminoacridines; C010847:9-amino-6-chloro-2-methoxyacridine; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases