Dehydroepiandrosterone is a substrate for estradiol 17 beta-dehydrogenase from human placenta.
The enzyme estradiol 17 beta-dehydrogenase (17 beta-ED) [E.C.1.1.1.62] from human placenta was purified to homogeneity by the initial steps of a published procedure, followed by an affinity chromatography step in Reactive Blue 2-Sepharose, eluting with NADP. The pure enzyme is not specific for estrogenic substrates, it also catalyzes the oxidation-reduction of several androgens and progesterone (i.e. dehydroepiandrosterone, androstenedione, 5 alpha-dihydrotestosterone, and 20 alpha-dihydroprogesterone). The comparison of the kinetic parameters for these substrates, shows that dehydroepiandrosterone could be a physiological ligand of the enzyme, and consequently involved in the control of its function in estrogen metabolism.
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000728:Androgens / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D013379:Substrate Specificity
D000728:Androgens; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases