Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells.
We show that extracts of rat islets of Langerhans and of cloned hamster beta-cells (HIT-T15 cells) contain Ca2+-phospholipid-dependent protein kinase and endogenous protein substrates for the kinase. We purified Ca2+-phospholipid-dependent protein kinase from HIT-T15 beta-cells and report here its physical and kinetic properties.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002850:Chromatography, Gel; D006224:Cricetinae; D007515:Islets of Langerhans / Q000201:enzymology*; D007700:Kinetics; D010743:Phospholipids / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C; D011494:Protein Kinases / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains
D010743:Phospholipids; D011494:Protein Kinases; D011493:Protein Kinase C; D002118:Calcium