TNP-enzyme conjugates for the detection of anti-TNP antibody producing cells in vivo.
After injection of TNP-KLH in mice and TNP-BGG-PEN in rabbits, anti-TNP antibody-forming cells were observed in the spleen. When cryostat sections of the stimulated spleen were incubated with TNP-HRP conjugate, and then treated for HRP cytochemistry, the cytoplasm of anti-TNP-forming cells was stained red. When similar sections were incubated with TNP-AP conjugate, and then treated for AP cytochemistry, the cytoplasm of anti-TNP-forming cells was stained blue. After simultaneous incubation with TNP-AP conjugate and PEN-HSA-HRP conjugate, followed by treatment for HRP cytochemistry and AP cytochemistry, anti-TNP-forming cells with a blue cytoplasm and anti-PEN-forming cells with a red cytoplasm could be distinguished in the same spleen section.
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000921:Antibody-Producing Cells / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D010397:Penicillanic Acid / Q000276:immunology; D010544:Peroxidases / Q000276:immunology*; D011817:Rabbits; D013154:Spleen / Q000166:cytology; D014301:Trinitrobenzenes / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D005719:gamma-Globulins; D010397:Penicillanic Acid; D006735:Horseradish Peroxidase; D010544:Peroxidases; D000469:Alkaline Phosphatase