Apoprotein B of lipoprotein(a) of human plasma.
Lipoprotein(a) was purified by agarose-gel chromatography from human plasma from which lipoproteins of Sf greater than 0 had been removed either by sequential or by density-gradient ultracentrifugation. After delipidation, the apoprotein B of this lipoprotein was analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. It could not be distinguished from the apoprotein B of low-density lipoproteins (rho 1.019-1.063 g/ml). A significant increase in the concentration of apoprotein B in plasma from which the Sf greater than 0 lipoproteins had been removed was observed in six subjects 4 h after a fatty meal.
D000328:Adult; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B; D002847:Chromatography, Agarose; D004041:Dietary Fats / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged
D001053:Apolipoproteins; D001055:Apolipoproteins B; D004041:Dietary Fats; D017270:Lipoprotein(a); D008074:Lipoproteins