Quantitation of urinary niacin metabolites by reversed-phase liquid chromatography.
The niacin metabolites N1-methyl-2-pyridone-5-carboxamide (2-PYR) and N1-methylnicotinamide (N-MN) have been quantified in human urine using isocratic reversed-phase high-performance liquid chromatography with ultraviolet detection after a simple anion-exchange clean-up procedure. The coefficients of variation (%) of the method for 2-PYR and N-MN were 6.3 and 6.8, respectively, and the limits of detection (mg/1 urine) were 0.05 for 2-PYR and 0.20 for N-MN. Results by this method compared favorably with those obtained by established techniques. It is a straight forward, rapid, and sensitive procedure for the assessment of niacin status.
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D009525:Niacin / Q000378:metabolism*; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000652:urine
D009536:Niacinamide; D009525:Niacin; C005211:N'-methyl-2-pyridone-5-carboxamide; C024058:N(1)-methylnicotinamide