Purification and partial characterization of an acidic fibroblast growth factor from bovine pituitary.
Isoelectric focusing has allowed us to fractionate pituitary extracts into basic (pI 8-9) and acidic (pI 4-5) fibroblast growth factor. The acidic fibroblast growth factor (a) is stable upon refocusing, (b) migrates as an acidic protein in urea-containing gel electrophoresis; (c) is not cell-specific, being active with fibroblasts, adrenal, and glial cells, and (d) is a heterogeneous protein fraction with active components of different pI values. The component of pI 4.7, purified to or near homogeneity by isoelectric focusing shows a single peak of activity (Mr = 12,000) in gel chromatography and a single protein band of apparent Mr = 15,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Maximal restimulation of DNA synthesis initiation on serum-deprived 3T3 fibroblasts is achieved at 1-2 ng/ml; activity with rat glial cells (C6-3D) is less pronounced than with 3T3 fibroblasts.
D000311:Adrenal Glands; D000818:Animals; D002417:Cattle; D002460:Cell Line; D005346:Fibroblast Growth Factors; D005347:Fibroblasts / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D051379:Mice; D008970:Molecular Weight; D009457:Neuroglia / Q000187:drug effects; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D010902:Pituitary Gland / Q000032:analysis*
D010455:Peptides; D005346:Fibroblast Growth Factors