Evidence of suppressor activity mediated by concanavalin-A stimulated cells towards aspecific LIF production.
Since the T-suppressor subpopulation is known to have a peculiar sensitivity to the mitogenic activity of Concanavalin-A we decided to verify whether in normal subjects it was possible to induce, by using the Con-A as a mitogenic factor, the proliferation of cells--possibly T-suppressor--which: a) had the property of adherence and b) show inhibitory activity toward LIF production. The statistical analysis of the results of our experimental patterns show that even if a difference between the suppression exerted on LIF production by Con-A stimulated versus not stimulated cells is present at any dilution, the level of significance rises from P less than 0.005 to P less than 0.0001 for the range of dilution 1:1/4 to 1:132, thus revealing that these experimental conditions are optimal for eliciting the blocking effect. The researches that are currently in progress in our laboratory seem to suggest that this experimental approach is of some value in characterizing the immunity pattern of laryngeal cancer patients and its evolution through the course of disease.
D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000187:drug effects*
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010835:Phytohemagglutinins; D003208:Concanavalin A