Renal localization of heparan sulfate proteoglycan by immunohistochemistry.
Glomerular localization of heparan sulfate proteoglycan (HS-proteoglycan) has been studied immunohistochemically with a highly purified antiserum to bovine aorta HS-proteoglycan core protein. The specificity of the antiserum was enhanced by consecutive fibronectin and chondroitin sulfate-dermatan sulfate proteoglycan (CS-DS proteoglycan) affinity chromatography. The affinity-purified HS-proteoglycan antibody lacked cross-reactivity by enzyme-linked immunosorbent assays (ELISA) with CS-DS proteoglycan, fibronectin, laminin, and Type IV collagen. Reactivity of the antiserum with HS-proteoglycan antigen by ELISA was inhibited by HS core protein derived from CsCl density gradient centrifugation after heparinase treatment of the HS-proteoglycan. Immunofluorescent reactivity of the HS-proteoglycan antiserum was observed with bovine glomerular basement membrane, renal interstitium, Bowman's capsule, renal arterioles, and bovine aorta. No staining was seen with rat, mouse, or human glomeruli.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001011:Aorta / Q000276:immunology; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000276:immunology*; D002809:Chondroitin Sulfates / Q000032:analysis; D003871:Dermatan Sulfate / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006025:Glycosaminoglycans / Q000276:immunology*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000276:immunology*; D006651:Histocytochemistry; D007120:Immunochemistry; D007678:Kidney Glomerulus / Q000032:analysis / Q000276:immunology*; D011509:Proteoglycans / Q000276:immunology*; D014020:Tissue Extracts
D000906:Antibodies; D011508:Chondroitin Sulfate Proteoglycans; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans; D014020:Tissue Extracts