Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin.
Apurinic sites were excised from phi X174 RF DNA with two enzymes isolated from rat liver chromatin: an apurinic/apyrimidinic endodeoxyribonuclease and a 5'-3'-exonuclease; the resulting gap was filled with DNA polymerase beta also prepared from rat liver chromatin and the repair was fully terminated with T4 ligase.
D000818:Animals; D001080:Apurinic Acid / Q000378:metabolism; D010584:Bacteriophage phi X 174 / Q000737:chemistry; D001665:Binding Sites; D002843:Chromatin / Q000201:enzymology*; D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D051381:Rats
D002843:Chromatin; D004279:DNA, Viral; D001080:Apurinic Acid; D004247:DNA; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase