[Comparative evaluation of fibrinogen and fibrin hydrolysis with plasmin].
A high-sensitive method is developed for determining the degree of plasmin-catalyzed fibrinogen hydrolysis by the released amino groups stained with trinitrobenzene sulphoacid. The method permits determining 0.02-0.08 casein units of plasmin. The method made it possible to establish that after streptokinase activation plasmin hydrolyzes equally fibrinogen and fibrin in solution and as gel. When a tissue activator is used, fibrin intensifies significantly the plasminogen activation. Inhibition of plasmin by an inhibitor produced from soya is considerably slowed down in fibrin gel.
D000818:Animals; D002417:Cattle; D005337:Fibrin / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis*; D005341:Fibrinolysin / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D010960:Plasminogen Activators / Q000032:analysis*; D013300:Streptokinase / Q000494:pharmacology; D013379:Substrate Specificity
D005337:Fibrin; D005340:Fibrinogen; D013300:Streptokinase; D010960:Plasminogen Activators; D005341:Fibrinolysin
Sravnitel'noe izuchenie gidroliza fibrinogena i fibrina plazminom.