title: Localization of pyruvate carboxylase in the cells of Neurospora crassa.
abstract: The cell wall of Neurospora crassa was digested enzymatically and the cytosolic and the mitochondrial fractions were separated. The activity of pyruvate carboxylase (EC 6.4.1.1) was detected entirely in the cytosolic fraction. This indicates that the location of pyruvate carboxylase of N. crassa is in the cytosol, but is not in the mitochondria; this is different from the situation in animal tissues.
mesh_terms: D000105:Acetyl Coenzyme A / Q000494:pharmacology; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology*; D005649:Fumarate Hydratase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000648:ultrastructure; D011766:Pyruvate Carboxylase / Q000378:metabolism*
chemical_list: D000105:Acetyl Coenzyme A; D002950:Citrate (si)-Synthase; D005649:Fumarate Hydratase; D011766:Pyruvate Carboxylase