Different biosynthetic pathways of the pyrimidine moiety of thiamin in procaryotes and eucaryotes.
[14C]Formate is incorporated into the C-2 of the pyrimidine moiety of thiamin by Escherichia coli and Salmonella typhimurium. In Saccharomyces cerevisiae, it is incorporated into C-4. Radioactive carbons of [1-14C]glycine and [2-14C]glycine are incorporated by S. typhimurium into the C-4 and C-6 of the pyrimidine, respectively, but not by S. cerevisiae. These facts suggest that procaryotes and eucaryotes have different biosynthetic pathways for pyrimidine. In this study, the procaryotes tested incorporated [14C]formate into the C-2 and the eucaryotes incorporated it into the C-4 of the pyrimidine.
D001233:Aspergillus nidulans / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004754:Enterobacter / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D005658:Fungi / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D007700:Kinetics; D009089:Mucor / Q000378:metabolism; D009492:Neurospora crassa / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism; D011743:Pyrimidines / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism; D013045:Species Specificity; D013831:Thiamine / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D005561:Formates; D011743:Pyrimidines; C030544:formic acid; D005998:Glycine; D013831:Thiamine