A quenched-flow apparatus which allows the measurement of the kinetics of a reaction in one stroke.
A chemical quenched-flow apparatus is described which measures, in a unique stroke, enough data points (8-11) for establishing the kinetics curve of a reaction. Only very small volumes of reaction solutions (2 X 500 microliters) are required. The time intervals between which the kinetic data may be measured range from 5 to 37 ms and from 120 to 450 ms with the corresponding mixing times of 0.6 and 5 ms, respectively. This apparatus was used to investigate the pre-steady-state domain of the aminoacylation reaction of tRNAVal by valyl-tRNA synthetase from yeast.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D007700:Kinetics; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012212:Rheology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014637:Valine-tRNA Ligase / Q000378:metabolism*
D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases; D014637:Valine-tRNA Ligase