Loose binding of testicular mitochondrial ATPase to the inner membrane.
Rat testis mitochondrial ATPase was not inhibited by oligomycin at pH 7.5. It was inhibited only at higher alkaline pH's, and showed a lower sensitivity both to oligomycin and N,N'-dicyclohexylcarbodiimide and a higher one to efrapeptin. In submitochondrial particles, testis ATPase was only slightly inhibited by oligomycin, ossamycin, and efrapeptin. The possibility of a loose binding of F1 to the membrane was supported by its recovery from the supernatant of the submitochondrial particles. Furthermore, by electron microscopy, after hypoosmotic shock and negative staining of the mitochondrial preparations, most of the inner mitochondrial membranes showed only a few "knobs" or none at all. The capacity of the testis mitochondrial preparation to produce ATP was tested and compared to that from liver. ATP synthetase/ATPase activity ratio was 30/1 in liver mitochondria, whereas in the testis it was 3/1. In spite of this large difference, at least part of the testis ATPase must be firmly bound to the membrane, since it is able to form ATP. The rest seems to be loosely bound and its functional significance is still unknown.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology; D018942:Macrolides; D008274:Magnesium / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology; D009840:Oligomycins / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000201:enzymology; D013737:Testis / Q000201:enzymology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D018942:Macrolides; D009840:Oligomycins; D010455:Peptides; C008717:ossamycin; D004024:Dicyclohexylcarbodiimide; C012953:efrapeptin; D000251:Adenosine Triphosphatases; D008274:Magnesium