A method to increase the volume of sample applied to isoelectric focusing gels.
Isoelectric focusing of extracts from diluted or aged bloodstains may be more successfully accomplished with larger sample volumes applied to the gel. A technique is described using teflon tubing to apply larger sample volumes (up to 100 microliters) to isoelectric focusing (IEF) gels. This method is reproducible and easy to perform.
D001801:Blood Stains; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D010733:Phosphoglucomutase / Q000032:analysis* / Q000235:genetics
D010733:Phosphoglucomutase