Increased synthesis of the phosphorylated form of the myosin light chains in cardiac hypertrophy in the rat.
The incorporation rate of [3H]leucine into cardiac myosin subunits was studied in rat hearts undergoing hypertrophy secondary to constriction of the ascending aorta. Cardiac myosin was prepared by a modified Shiverick's method on the second and fourth day after constriction. Myosin light chains were separated by urea and subjected to two-dimensional electrophoresis. Incorporation of [3H]leucine was determined in electrophoretically separated heavy and light chains by the method of Martin et al. (11). It was found that the incorporation rate of [3H]leucine into the phosphorylated form of the myosin light chain 2 is significantly increased in hypertrophic heart as compared to sham animals.
D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism*; D003250:Constriction; D005260:Female; D007930:Leucine / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D009218:Myosins; D007930:Leucine