The role of myelin lipids in experimental allergic encephalomyelitis. Part 1. Influence on disease production by non-encephalitogenic doses of myelin basic protein.
Hartley guinea pig central nervous system (CNS) myelin has been purified and fractionated into its protein and lipid components. Experimental allergic encephalomyelitis (EAE) was induced in juvenile strain 13 guinea pigs with both lyophilized and fresh 'wet' myelin. However, a larger dose of lyophilized myelin was required to induce chronic EAE. Total myelin lipids, galactocerebrosides, gangliosides, phospholipids or proteolipids were combined with a non-encephalitogenic dose of myelin basic protein (MBP) and injected in juvenile Hartley guinea pigs. No clinical or histological manifestations of disease were observed. Parameters of immune functions indicated that the total myelin lipids augmented cell-mediated immune responses as measured by in vitro lymphocyte transformation and by a significant decrease in the percentage of peripheral early T cells. Only the proteolipids elicited delayed hypersensitivity reactions. Animals that received the phospholipid-MBP combination showed no changes when compared to animals injected with MBP alone. The results suggest that although the myelin lipids did not act synergistically with a non-encephalitogenic dose of MBP to induce EAE, they induced immunological changes and potentiated the immune response to MBP.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002498:Centrifugation; D002855:Chromatography, Thin Layer; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced*; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000139:chemically induced; D008055:Lipids / Q000502:physiology; D008213:Lymphocyte Activation; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D009186:Myelin Sheath / Q000378:metabolism*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D000906:Antibodies; D008055:Lipids; D004676:Myelin Basic Protein