[Isolation from a pregnant woman of a serum protein responsible for inhibiting an in vitro cytotoxicity reaction].
A non specific immunosupressive factor able to block an in vitro cytotoxicity reaction is demonstrated in the serum of pregnant women. A solution containing this blocking factor is obtained by gel filtration and precipitation of plasma by polyethylene glycol 4 000. Then after immunisation of rabbits the immune serum can be used for affinity chromatography. An alpha 2 glycoprotein has been separated which inhibits the in vitro cytotoxicity reaction and whose molecular weight determined by gel filtration and polyacrylamide gel is about 200 000 daltons.
D001798:Blood Proteins / Q000302:isolation & purification / Q000502:physiology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification* / Q000502:physiology
D001798:Blood Proteins; D007166:Immunosuppressive Agents; D011257:Pregnancy Proteins
Isolement chez la femme enceinte d'une protéine sérique responsable de l'inhibition d'une réaction de cytotoxicité in vitro.