Differential reactivity of human lymphocytes allosensitized in vitro in hormonally defined medium or medium supplemented with plasma.
We compared the in vitro allogeneic response of human lymphocytes cultured in hormonally defined medium (HDM) with that of those cultured in plasma-containing medium (PCM). Differences occurred only in secondary MLR or PLT and in cell-mediated cytotoxicity in terms of specificity, which was either greater or modified when HDM was used.
D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D003470:Culture Media; D008222:Lymphokines