[Analysis of the serodiagnostic reactions of the bacterium Clostridium butyricum strain CNRZ 528 using passive hemagglutination].
For serologic tumour diagnostics a specific proof of antibodies against rods of Clostridium butyricum CNRZ 528 by passive hemagglutination has been described. We were able to prepare an antigen from the cell extract (crude-antigen) of rods without flagella by gel filtration, that after fixation on the surface of human-erythrocytes, stabilized by tannin or glutaraldehyde, agglutinates specifically with rod-antibodies of rabbit-hyperimmunserum. Simultaneously however, there was no cross-reactivity with spore-antibodies of rabbit-hyperimmunserum. Sensitivity and specificity of this test are dependent on an optimal dose of antigen bound to the surface of the erythrocytes. The optimization of the assay has to be achieved by the testing of patients sera.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001666:Binding Sites, Antibody; D003013:Clostridium / Q000235:genetics / Q000276:immunology; D003015:Clostridium Infections / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D005779:Immunodiffusion; D009369:Neoplasms / Q000175:diagnosis; D011817:Rabbits
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Untersuchungen zur Serodiagnostik von Stäbchen des Stammes Clostridium butyricum CNRZ 528 mit Hilfe der passiven Hämagglutination.