Suppression of mixed lymphocyte culture by uremic adherent spleen cells and peritoneal macrophages is dependent on a cyclophosphamide-sensitive cell.
A model of experimentally induced uremia in the rat has been used to study the effect of uremia on the response of spleen cells to alloantigens. The proliferative ability of uremic spleen cells in mixed lymphocyte culture is significantly suppressed when compared to that of cells from control animals. This suppression appears to be due to both adherent suppressor cells which can be eliminated by adherence to rayon wool and to the inability of uremic T cells to respond to alloantigens. In addition, unstimulated peritoneal macrophages ( PMO ) obtained from uremic rats were also shown to be suppressive to the response of control spleen cells to alloantigens. The suppression by uremic adherent spleen cells and PMO is regulated by cyclophosphamide-sensitive cells.
D000818:Animals; D002448:Cell Adhesion; D003520:Cyclophosphamide / Q000494:pharmacology; D007108:Immune Tolerance; D007111:Immunity, Cellular / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014511:Uremia / Q000276:immunology*
D003520:Cyclophosphamide