title: Distance from cohesive end site cos determines the replication requirement for recombination in phage lambda.
abstract: Previous work showed that crossing-over in the middle of the chromosome of phage lambda requires more DNA replication than does crossing-over near the termini. Relocation of cos, the sequence that determines the lambda termini, alters the requirements for replication in a given marked interval, demonstrating that distance from cos determines the amount of DNA replication that is required for genetic exchange. This result supports a break and copy mechanism for recombination mediated by the red system of phage lambda.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D010582:Bacteriophage lambda / Q000235:genetics*; D002874:Chromosome Mapping; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D011938:Rec A Recombinases; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D011938:Rec A Recombinases