[Reconstitution of the function of the coupling complex of chloroplasts on phospholipid vesicles].
The function of H+-ATPase (CF1-CF0) complex from spinach chloroplasts was reconstituted on azolectin liposomes. Using these proteoliposomes, the 32P-ATP exchange reaction sensitive to dicyclohexylcarbodiimide and uncouplers was established. Combined incorporation of H+-ATPase and bacteriorhodopsin as a delta mu H+ inducer results in photophosphorylation. The optimal conditions for production of liposomes with a low permeability of membranes for ions were elaborated with a view towards improving the existing reconstitution methods.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001436:Bacteriorhodopsins / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology*; D007700:Kinetics; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D010785:Photophosphorylation; D010944:Plants / Q000201:enzymology; D011510:Proteolipids; D006180:Proton-Translocating ATPases / Q000378:metabolism*
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011510:Proteolipids; C020485:proteoliposomes; D001436:Bacteriorhodopsins; C024990:asolectin; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases
O rekonstruktsii funktsii sopriagaiushchego kompleksa khloroplastov na fosfolipidnykh vesikulakh.