Double immunofluorescence studies of immunoglobulins, complement components and their control proteins in patients with IgA nephropathy.
A study on double immunofluorescent staining of immunoglobulins, complement components, and their control proteins in renal tissues from patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy. These biopsy specimens were stained with anti sera to human C1q, C4-binding protein, and beta 1H globulin by indirect immunofluorescent staining. These samples were then stained with rhodamine-conjugated anti human IgG, IgM, and IgA. Although C1q and C4-binding protein do not combine with IgA, they ubiquitously combine with IgG and/or IgM. beta 1H globulin also combine with IgG, IgM and/or IgA. It was demonstrated that the complement system activated in IgA nephropathy was via both alternative and classical pathways. The presence of C4-binding protein in glomeruli appeared to be a more sensitive indicator of classical pathway activation than the presence of C4.
D002352:Carrier Proteins / Q000276:immunology; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D003176:Complement C3 / Q000276:immunology; D003180:Complement C3b Inactivator Proteins / Q000276:immunology; D017242:Complement Factor H; D003165:Complement System Proteins / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D016167:Integrin alphaXbeta2; D007674:Kidney Diseases / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology*
C493495:CFH protein, human; D002352:Carrier Proteins; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D016167:Integrin alphaXbeta2; D015922:Complement C1q; D017242:Complement Factor H; D003165:Complement System Proteins; D003166:Complement Activating Enzymes