Androgen dependence of protein N-glycosylation in rat epididymis.
The rat epididymis is known to produce and secrete glycoproteins which interact with spermatozoa during the maturation process. The synthesis of the protein core of these compounds is dependent on androgenic stimulation. As a consequence, we studied the possible androgenic control of the N-glycosylation process dependent on the dolichol (Dol) pathway. Glucosyl and mannosyl transferase activities in rat epididymal microsomes decreased by approximately 76% after only 2 days of castration with respect to intact controls. Depleted mannosyl transferase activity could be restored to control values by administration of 100 micrograms/day testosterone propionate (TP) for 4 days. The effect of 20 micrograms/day TP was blocked by the simultaneous administration of 500 micrograms/day of the antiandrogen cyproterone acetate. The addition of excess dolichyl phosphate (12 times the Michaelis-Menten constant (Km) value) to the incubation mixture did not eliminate the difference in mannosyltransferase activity between epididymal microsomes from castrated rats and these from control or testosterone-treated animals. Moreover, the endogenous pool of dolichyl phosphate was found unchanged in the different hormonal situations. Finally, the incorporation of [14C]mannose into lipid-bound oligosaccharides and into glycoproteins was decreased by approximately 60% as a result of castration and reinduced to control values by treatment with TP (50 micrograms/day for 4 days). The results demonstrate the androgen dependence of the initial steps of N-glycosylation in the rat epididymis and suggest that the hormonal regulation is exerted at the level of Dol-nucleotide sugar transferases, rather than upon the size of the endogenous Dol phosphate pool.
D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D002369:Castration; D003534:Cyproterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D017373:Cyproterone Acetate; D004822:Epididymis / Q000187:drug effects / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008364:Mannosyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology*
D000726:Androgen Antagonists; D006023:Glycoproteins; D013739:Testosterone; D017373:Cyproterone Acetate; D003534:Cyproterone; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D008364:Mannosyltransferases; C035777:chitobiosyldiphosphodolichol beta-mannosyltransferase