DNA content of macrophages in pulmonary granuloma induced in rats by complete Freund's adjuvant.
Pulmonary granulomas were induced in rats with complete Freund's adjuvant. Histologic and cytologic alterations and Feulgen-DNA amounts determined by a cytophotometric method were studied. Fourteen (hours after injection, the pulmonary interstitia were thickened by cell infiltration. After 3 days; small granulomatous nodules developed containing macrophages, epithelioid and giant cells. The Feulgen-DNA amounts increased significantly in the course of the first day and reached peak values on the 3rd day. Then they showed a tendency to decrease after the 7th day. The present results confirm the previously obtained data, revealing the stimulating effect of the complete Freund's adjuvant on cell metabolism, DNA synthesis included.
D000818:Animals; D004247:DNA / Q000378:metabolism*; D005620:Freund's Adjuvant; D006099:Granuloma / Q000209:etiology / Q000473:pathology*; D008171:Lung Diseases / Q000209:etiology / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism*; D051381:Rats
D004247:DNA; D005620:Freund's Adjuvant