title: T4 DNA ligase can seal a nick in double-stranded DNA limited by a 5'-phosphorylated base-free deoxyribose residue.
abstract: The 5' AP endodeoxyribonucleases hydrolyze the phosphodiester bond 5' to AP (apurinic or apyrimidinic) sites in double-stranded DNA leaving 3'-OH and 5'-phosphate ends. These nicks are sealed by T4 DNA ligase although the 5'-phosphate end belongs to a base-free deoxyribose.
mesh_terms: D000818:Animals; D011088:DNA Ligases / Q000378:metabolism*; D004279:DNA, Viral; D003850:Deoxyribonuclease I; D003855:Deoxyribose; D004706:Endodeoxyribonucleases / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010766:Phosphorylation; D011118:Polynucleotide Ligases / Q000378:metabolism*; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology*
chemical_list: D004279:DNA, Viral; D003855:Deoxyribose; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D011088:DNA Ligases; D011118:Polynucleotide Ligases