The effect of theophylline on macrophage elastase secretion.
Theophylline increased the elastolytic activity in conditioned media from murine peritoneal macrophages maintained in a monolayer system. Continuing accumulation of enzyme activity with time required the continuing presence of the drug. Theophylline was active in the presence of a maximally stimulatory concentration of colchicine, a known enhancer of elastase secretion. The effect of theophylline was not due to interference with any elastase inhibitory activity in macrophage conditioned medium. The elastolytic activity from theophylline-stimulated cells exhibited an inhibitor profile similar to the profiles of colchicine-stimulated and unstimulated cells. The results demonstrate that theophylline regulates the amount of extracellular elastolytic activity from murine peritoneal macrophages differently from granulocytic elastase.
D000818:Animals; D002478:Cells, Cultured; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D012967:Sodium Dodecyl Sulfate; D013806:Theophylline; D010196:Pancreatic Elastase; D014357:Trypsin