Induction of expression and phosphorylation of the human interleukin 2 receptor by a phorbol diester.
The receptor for interleukin 2 (IL 2) is induced on normal mature T cells activated by antigen or mitogen. Receptor-negative human T leukemic cell lines were induced to express the IL 2 receptor after incubation with 12-O-tetradecanoyl phorbol 13-acetate. The Mr of the IL 2 receptor expressed on the surface of four different T cell lines and normal peripheral blood lymphocytes varied. The source of the heterogeneity appeared to be due to differences in post-translational processing of the receptor. The precursor of the IL 2 receptor isolated from tunicamycin-treated HUT102B2 or 12-O-tetradecanoyl phorbol 13-acetate-induced CCRF-CEM cells had the same Mr and isoelectric point. Incubation of cells with 12-O-tetradecanoyl phorbol 13-acetate also induced the rapid phosphorylation of serine and threonine residues on the IL 2 receptor from HUT102B2 cells and activated peripheral blood lymphocytes. This phosphorylation may be mediated by the Ca2+ and phospholipid-dependent protein kinase C.
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005434:Flow Cytometry; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D010766:Phosphorylation; D011493:Protein Kinase C; D011494:Protein Kinases / Q000378:metabolism; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D015375:Receptors, Interleukin-2; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
D010704:Phorbols; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D014415:Tunicamycin; D011494:Protein Kinases; D011493:Protein Kinase C; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D014357:Trypsin; D013755:Tetradecanoylphorbol Acetate