An improved solid-phase immunoassay for anti-GBM antibodies.
A solid-phase immunoassay has been developed for the detection of anti-glomerular basement membrane (GBM) antibodies in the circulation of patients with Goodpasture's syndrome and one form of rapidly progressive glomerulonephritis. The procedure involves the formation of a covalent linkage between the antigen and a solid support disc. Antibodies bound to the antigen are then detected on a fluorometer using fluorescein-labeled antisera to human immunoglobulins. The assay is easy to perform, fast, inexpensive and has been optimized with respect to sensitivity, range and reproducibility.
D000906:Antibodies / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology*; D006801:Humans; D007105:Immune Complex Diseases / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007678:Kidney Glomerulus / Q000648:ultrastructure
D000906:Antibodies