ABA-specific helper T cells in A/J mice bear the major cross-reactive idiotype.
Attempts were made to induce azobenzenearsonate (ABA)-specific helper cell responses in A/J mice. These were measured by an increase in TNP plaque-forming cells following administration of the double hapten conjugate ABA-bovine serum albumin-TNP. Immunization with ABA coupled homologous immunoglobulin or spleen cells produced ABA-specific help only when the same carrier was used to boost. Hapten-specific help was achieved by two injections of ABA-N-acetyltyrosine in complete Freund's adjuvant 5 weeks apart. This help was passively transferable by T cells as shown by its elimination with anti-Thy 1.2 serum and complement treatment. The presence of the major ABA cross-reactive idiotype (CRI) on these T helper cells could be similarly shown by the elimination of help when the cells were treated with rabbit anti-CRI antibody and complement prior to passive transfer. The same treatment did not effect ABA-specific helper activity of CBA/J mice.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006241:Haptens / Q000276:immunology*; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008808:Mice, Inbred CBA; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D014443:Tyrosine; D010132:p-Azobenzenearsonate; C025787:N-acetyltyrosine