Inhibition of T and B lymphoblastic response by mithramycin, dacarbazine, prospidium chloride and peptichemio.
Four different cytostatic compounds (prospidium chloride, peptichemio, dacarbazine and mithramycin) have been assayed for their effect on lymphoblastic transformation of spleen cells from mice. The drugs did not affect cell viability at concentrations lower than 0.1-0.2 micrograms/ml (peptichemio and mithramycin) or than 10-20 micrometers/ml (prospidium chloride and dacarbazine). Except for peptichemio, which did not show any marked effect, these cytostatics acted more actively on B cells than on T cells at concentrations of drug not affecting lymphocyte viability. Mithramycin was the most active inhibitor of the mitogen-induced DNA synthesis in the cell. Concentrations of this drug to inhibit the blastogenic response to 50% (IC50) were lower than 0.1 microgram/ml. Inhibition of mitogenesis was less pronounced in the case of dacarbazine (IC50 = 50 and 10 micrograms/ml for T and B cells, respectively), prospidium chloride (IC50 greater than 50 and 50 micrograms/ml for T and B cells) and peptichemio (IC50 = 0.25 and 0.5 microgram/ml for T and B cells, respectively.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002470:Cell Survival; D002478:Cells, Cultured; D003606:Dacarbazine / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008558:Melphalan / Q000031:analogs & derivatives*; D051379:Mice; D008807:Mice, Inbred BALB C; D010440:Peptichemio / Q000008:administration & dosage / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D008926:Plicamycin / Q000008:administration & dosage / Q000494:pharmacology*; D011447:Prospidium / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects*
D010879:Piperazines; D003606:Dacarbazine; D011447:Prospidium; D010440:Peptichemio; D008926:Plicamycin; D008558:Melphalan