[Directed modification of the damaged DNA region in inducing structural chromosomal mutations].
The method of directed modification of damaged DNA regions elaborated recently in our laboratory made it possible to conduct a direct experimental study of mutagenicity of two single-stranded breaks located within the isolocus of sister chromatids, in strands of the same polarity. The efficiency of induced mutagenesis was shown to be very high when the experimental technique was employed allowing single-stranded DNA breaks to occur in this position with high probability. It should be noted that the fraction of interstitial deletions and rearrangements of chromosomes of the exchange type were predominant in the spectrum of induced mutations. It is concluded that the coincidence of two breaks in the strands of the same polarity in the isolocus of sister chromatids is realised into chromosome aberrations with high probability.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D005467:Floxuridine / Q000494:pharmacology; D066298:In Vitro Techniques; D009154:Mutation; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D005467:Floxuridine; D001973:Bromodeoxyuridine
Napravlennaia modifikatsiia povrezhdennogo uchastka DNK v induktsii strukturnykh mutatsiĭ khromosom.