Calcium transport, ATPase activity and lipid composition in sarcoplasmic reticulum isolated from isogenic lines of normal and dystrophic chickens.
Two new lines of chickens with near identical genotypes (greater than 90% isogeneity), one demonstrating avian dystrophy, were used for isolation of sarcoplasmic reticulum vesicles. Vesicles from line 433 (dystrophic) displayed reduced Ca2+-ATPase activity, phosphoenzyme formation and steady-state calcium transport capabilities in comparison with vesicles from line 03 (normal). Lipid analyses show that dystrophic vesicles have greater amounts of cholesterol and lesser amounts of phosphatidylcholine. The results support the use of isogenic chickens in further studies of avian dystrophy. However, the results also suggest that current sarcoplasmic reticulum vesicle purification procedures dependent on differential calcium accumulation may not fully achieve the intended purpose.
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002645:Chickens; D002784:Cholesterol / Q000378:metabolism; D005838:Genotype; D008563:Membrane Lipids / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D000252:Calcium-Transporting ATPases; D002118:Calcium