Immunoreactivity of rhodopsin and opsin.
An examination by a radioimmunoassay of the relative affinity of opsin and rhodopsin for rabbit antibody raised against bovine rhodopsin revealed that opsin was the preferred antigen. About 10-fold greater amounts of rhodopsin than opsin were required to achieve 50% inhibition of binding of 125I-labeled ligand in the RIA. Opsin was more reactive when examined in the light or dark, compared to rhodopsin incubated in the dark. Mixtures of opsin and rhodopsin (prepared by partial bleaching of rhodopsin or synthetic mixtures) exhibited increased reactivity with increasing mole fraction of opsin. This response was nonlinear, with small increases in opsin producing relatively large increases in reactivity. A partial fractionation of the antibody into two groups showing differential reactivities toward opsin and rhodopsin was achieved by affinity chromatography on opsin-Sepharose. However, with both groups, opsin was still the preferred antigen. Scatchard analysis of 125I-labeled rhodopsin and opsin produced nonlinear plots, indicating the presence of multiple species of antibody. The affinities and binding capacities were similar for both labeled antigens. In competitive binding studies, the antibody showed a strong preference for either labeled ligand (rhodopsin or opsin) as compared to the unlabeled material. These latter observations indicate that altering rhodopsin either by bleaching or iodination produced changes in the relative immunoreactivity of the molecule.
D000818:Animals; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005136:Eye Proteins / Q000276:immunology*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007700:Kinetics; D011817:Rabbits; D011863:Radioimmunoassay; D012168:Retinal Pigments / Q000276:immunology*; D012243:Rhodopsin / Q000276:immunology*; D017299:Rod Opsins
D005136:Eye Proteins; D007457:Iodine Radioisotopes; D012168:Retinal Pigments; D017299:Rod Opsins; D012243:Rhodopsin