[Regulation of hydroxylation in position 21 of steroids in adrenal cortex cells of newborn rats, in culture. Utilization of aminoglutethimide and of exogenous steroids].
The use of aminoglutethimide at 5.10(-4) mol/l, inhibitor of endogenous steroidogenesis, and of exogenous progesterone (20 micrograms/ml) as substrate allowed the control of the steroid 21-hydroxylating reaction in cultured newborn rat adrenocortical cells. This method has been employed to show that the 21-hydroxylation by these cells is strongly induced by the adrenocorticotropic hormone (20 mU/ml).
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000616:Aminoglutethimide / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D006900:Hydroxylation; D007700:Kinetics; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D013255:Steroid 21-Hydroxylase / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D000616:Aminoglutethimide; D011374:Progesterone; D013250:Steroid Hydroxylases; D013255:Steroid 21-Hydroxylase
Etude de la régulation de l'hydroxylation en position 21 des stéroïdes dans des cellules corticosurrénaliennes de rats nouveau-nés, en culture. Utilisation de l'aminoglutéthimide et de stéroïdes exogènes.