High-performance liquid chromatographic determination of piperacillin in plasma.
A high-performance liquid chromatographic method has been developed for the quantitative determination of piperacillin in plasma. The compound is first extracted from acid-treated plasma into chloroform: 1-pentanol (3:1) and reextracted into a small volume of an aqueous phase at pH 7, which is injected in the chromatographic column (microgram-Bondapack C18; 10 micrometers). The mobile phase is a mixture of 0.01 M acetate buffer (pH 4.8) and methanol. The method is accurate and reproducible with a sensitivity of about 50 ng of piperacillin per ml of serum.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood; D002511:Cephalosporins / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D010406:Penicillins / Q000097:blood*; D010878:Piperacillin
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D010406:Penicillins; D010878:Piperacillin