Bioassay procedures for norfloxacin.
A disk-diffusion bioassay procedure for the quantitation of norfloxacin in serum, tissue and urine employing Klebsiella pneumoniae ATCC 10031 was developed. When incubated at 28 degrees C, the assay has a sensitivity of 0.2 microgram/ml. Zones of inhibition on assay plates may be measured by hand (ruler, vernier caliper) or by automated image analysis. Urine samples require an initial 1:2 dilution in 0.1N HCl before assay to assure complete solution of the compound. Bioassay values in urine are comparable to those obtained by high performance liquid chromatography.
D000900:Anti-Bacterial Agents / Q000032:analysis*; D001681:Biological Assay; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D009268:Nalidixic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000633:toxicity; D009643:Norfloxacin
D000900:Anti-Bacterial Agents; D009268:Nalidixic Acid; D009643:Norfloxacin