title: Salient characteristics of bovine pituitary intraglandular colloid: a transport medium for determinants to pituitary hormones and immunoglobulins.
abstract: Bovine hypophyseal intraglandular colloid, the holocrine secretion of marginal intermediate lobe cells, contains high molecular weight (MW 45 000 to 158 000) protein fractions and low molecular weight (MW below 25 000) peptide fractions. The fractions display immunoreactive determinants similar to those in pituitary hormones, cerebrospinal fluid (CSF), serum IgG and albumin. Immunoelectrophoresis shows that components in high molecular weight fractions distribute themselves in three distinct bands (gamma, beta, alpha), while those in low molecular weight fractions distribute themselves in two bands (gamma and alpha). Physiochemical characteristics, i.e., sedimentation rate, percentage of hexose, total CHO and 12C as well as the content of cystine are presented. It is concluded that pituitary colloid should not be dismissed as a waste product of cellular degeneration, since there are strong suggestions that it serves as a transport medium for certain pituitary hormones.
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002417:Cattle; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D003102:Colloids / Q000378:metabolism; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008970:Molecular Weight; D010902:Pituitary Gland / Q000378:metabolism*; D010907:Pituitary Hormones / Q000378:metabolism*
chemical_list: D000418:Albumins; D003102:Colloids; D007074:Immunoglobulin G; D007136:Immunoglobulins; D010907:Pituitary Hormones