Removal of degradation products from calf thymus high mobility group non-histone chromatin proteins by chromatography on immobilized double-stranded DNA.
The substantial protease activity in calf thymus chromatin inevitably produces some degradation of high mobility group (HMG) non-histone proteins in NaCl extracts of calf thymus chromatin. We have found that proteins considered to be degradation products can be conveniently and cleanly separated from intact high mobility group proteins 1 and 2 by chromatography on double-stranded DNA-cellulose in 0.2 M NaCl/1 mM Tris-HCl (pH 7.5). Under those conditions, only the presumptive degradation products are retained by the column.
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004247:DNA; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D010446:Peptide Fragments / Q000302:isolation & purification*; D013950:Thymus Gland / Q000032:analysis
D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D010446:Peptide Fragments; D004247:DNA