New approach to metabolism of 5'-deoxy-5-fluorouridine in humans with fluorine-19 NMR.
The metabolism of 5'-deoxy-5-fluorouridine (5'dFUrd), an antitumor fluoropyrimidine, has been investigated in human biofluids (blood, plasma, urine) using a new method: fluorine-19 NMR spectrometry. This method allows direct study of the biological sample and simultaneous identification of all the fluorinated metabolites. In the blood of a patient treated with 5'dFUrd during a 6-h continuous perfusion, we observed unmetabolized 5'dFUrd, 5-fluorouracil, 5,6-dihydrofluorouracil, and another metabolite which has not previously been reported alpha-fluoro-beta-alanine. The two major metabolites in urine are unmetabolized 5'dFUrd and alpha-fluoro-beta-alanine.
D000970:Antineoplastic Agents / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005260:Female; D005467:Floxuridine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005461:Fluorine; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000188:drug therapy / Q000556:secondary; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010477:Perfusion; D014594:Uterine Neoplasms / Q000188:drug therapy / Q000378:metabolism
D000970:Antineoplastic Agents; D005467:Floxuridine; D005461:Fluorine; C025034:doxifluridine