Characterization of the third component of pig complement and its utilization in a C3b receptor study.
The third component of the pig complement system (C3) was isolated in hemolytically active form and characterized. The C3 component is a beta-globulin with the molecular weight of 191,000 and is composed of 2 non-identical polypeptide chains of Mr 112,000 and 74,000. The isolated C3 can be used for the detection of the C3b receptor on the membranes of heterologous peritoneal macrophages.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002462:Cell Membrane / Q000032:analysis; D003176:Complement C3 / Q000276:immunology / Q000302:isolation & purification*; D007122:Immunoelectrophoresis; D008264:Macrophages / Q000032:analysis; D008970:Molecular Weight; D011951:Receptors, Complement / Q000032:analysis*; D017463:Receptors, Complement 3b; D013552:Swine
D000906:Antibodies; D003176:Complement C3; D011951:Receptors, Complement; D017463:Receptors, Complement 3b