title: Vanadate-resistant mutants of Neurospora crassa are deficient in a high-affinity phosphate transport system.
abstract: Mutant strains of Neurospora crassa have been selected which grow on media containing vanadate, an inhibitor of the plasma membrane ATPase. The mutations all map to a single region (designated van) on the left arm of linkage group VII. The van mutants are unable to take up vanadate from the medium and are also deficient in the uptake of phosphate via a derepressible, high-affinity phosphate transport system. In the van mutants, the K(m) for phosphate transport is elevated as much as 35-fold, indicating that the van locus may code for a structural component of the high-affinity phosphate transport system.
mesh_terms: D001692:Biological Transport; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D005796:Genes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics*; D010710:Phosphates / Q000378:metabolism*; D014638:Vanadates; D014639:Vanadium / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D010710:Phosphates; D014639:Vanadium; D014638:Vanadates