[Slide test for immunofluorescent microscopy and immunoenzyme detection of cell-membrane antigens].
Poly-dimethyl-diallyl-ammoniumchloride - coated glass slides cause a strong adherence of living cells in consequence of electrostatic interactions. Immunological reactions of adherent cells - we used immunofluorescence and immunoperoxidase techniques to demonstrate membrane antigens - are not impaired. The slide test is suitable for routine quantitation of B and T cells in the peripheral blood and for the selection of hybridoma antibodies against cellular antigens. The slide test is time saving and needs minimal volumes of cells and antisera.
D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000379:methods*
D000954:Antigens, Surface
Objektträgertest zur immunfluoreszenzmikroskopischen und enzymimmunologischen Erfassung von Zellmembranantigenen.