Siderophore production by Proteus mirabilis.
Studies on the isolation and characterization of Proteus mirabilis siderophores provided no evidence that these bacteria synthesize catechol- or hydroxamate-type siderophores. However, gas chromatograph analysis in conjunction with mass spectroscopy revealed the presence of alpha-hydroxyisovaleric acid, a previously unknown metabolite. Additional substantiating evidence for the presence of alpha-hydroxyisovaleric acid in these bacteria was obtained from experiments involving the use of thin-layer chromatography and an ultraviolet absorption spectrum. This compound was found to be capable of removing iron from the synthetic chelator, ethylene-diamine-di-orthohydroxyphenyl acetic acid, and supplying that iron to the bacteria both in a solid agar medium and in a liquid medium. Proteus mirabilis was found to possess an enzyme capable of catalyzing the reaction by which alpha-hydroxyisovaleric acid is converted to alpha-ketoisovaleric acid, an intermediate in the valine biosynthetic pathway.
D000818:Animals; D001437:Bacteriuria / Q000382:microbiology; D004285:Dogs; D045782:Hemiterpenes; D006736:Horses; D006801:Humans; D007476:Ionophores / Q000302:isolation & purification* / Q000378:metabolism; D007502:Iron Chelating Agents / Q000302:isolation & purification* / Q000378:metabolism; D007651:Keto Acids / Q000302:isolation & purification / Q000378:metabolism; D011512:Proteus Infections / Q000382:microbiology; D011513:Proteus mirabilis / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores; D014631:Valerates / Q000302:isolation & purification / Q000378:metabolism*
D045782:Hemiterpenes; D007476:Ionophores; D007502:Iron Chelating Agents; D007651:Keto Acids; D017262:Siderophores; D014631:Valerates; C035864:2-hydroxyisovaleric acid; C001505:alpha-ketoisovalerate