[Detection of stimulator-induced cytotoxicity of human mononuclear cells in short-term culture].
A new test modification for the detection of stimulant-induced cytotoxicity of human mononuclear blood cells is described. Papain-treated human erythrocytes were used as indicator cells. The effector cells were lymphocytes. The ratio of target cells to effector cells was 10/1. Haemoglobin as a marker of lysis of the erythrocytes, released in the supernatant, was measured quantitatively in form of its pseudoperoxidase-activity. PHA, ConA and tannic acid were ascertained and tested as stimulants of cytotoxicity. The reaction was inhibitable by anti-human-lymphocyte-globulin. The test conditions were optimized in regard to incubation time, -temperature, -vessels, culture medium and target cells. The technique is easy to manipulate, has only slight pretensions to the equipment of the laboratory and appears to be very effective. We recommend to apply this method of stimulant-induced cytotoxicity within the detection of the immune state, especially in the progress of immunopathological diseases and the analysis of efficiency of immunosuppressive therapy.
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013634:Tannins / Q000494:pharmacology*
D010835:Phytohemagglutinins; D013634:Tannins; D003208:Concanavalin A
Untersuchungen zum Nachweis der stimulatorinduzierten Zytotoxizität von mononuklearen Zellen des Menschen in der Kurzzeitkultur.