Isolation and fast purification of neocarzinostatin by FPLC-ion exchange chromatography.
Neocarzinostatin, a highly toxic antitumor protein containing an essential nonprotein chromophore, can be isolated and purified from culture filtrates of Streptomyces carcinostaticus. Usually a lengthy procedure of up to 60 h is necessary for the isolation, including several chromatographic steps partly under conditions which favour inactivation of the drug by release of chromophore. We describe a new method yielding practically clinical grade Neocarzinostatin from crude extracts in 20 min. This very fast and reproducible method was made possible by using a Mono Q anion exchange column filled with monodisperse gel material which has been recently developed.
D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification*; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013171:Spores, Bacterial / Q000187:drug effects; D013302:Streptomyces / Q000378:metabolism; D009353:Zinostatin / Q000032:analysis / Q000302:isolation & purification*
D000903:Antibiotics, Antineoplastic; D009353:Zinostatin