High-performance liquid chromatography for assaying NAD glycohydrolase from Neurospora crassa conidia.
A rapid and sensitive high-performance liquid chromatographic technique was developed to determinate NAD glycohydrolase (EC 3.2.2.5.) activity from Neurospora crassa conidia. The separation of the assay substrate and products was achieved by isocratic reverse-phase chromatography and the peaks were detected by the absorbance at 259 nm. Quantities of NAD+ and nicotinamide as small as 10 pmol could be measured.
D002851:Chromatography, High Pressure Liquid; D009699:N-Glycosyl Hydrolases / Q000032:analysis*; D009244:NAD+ Nucleosidase; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase