Inhibition of macrophage C3b-mediated ingestion by syphilitic hamster T cell-enriched fractions.
Macrophages are important for host defense against syphilitic infection. Our results show that C3b-mediated ingestion (C3bMI), a characteristic of activated macrophages, was inhibited in vitro by nonadherent cells from hamsters infected with Treponema pallidum subspecies endemicum. When macrophage target cells from normal, syphilitic, or lipopolysaccharide-treated animals were co-cultured with nonadherent cells derived from normal or syphilitic hamsters, noticeable differences were detected. Nonadherent syphilitic cells significantly suppressed macrophage C3bMI, whereas normal nonadherent cells displayed little or no suppressive activity. In general, macrophage C3bMI was reduced by nonadherent cells obtained throughout the course of syphilitic infection, although it eventually began to recover. The syphilitic nonadherent cells with maximum suppressive effect were those obtained from hamsters infected for 3 to 6 wk. The addition of treponemal antigens additionally inhibited C3bMI. The inhibitory effect of syphilitic nonadherent cells on either lipopolysaccharide-treated or syphilitic macrophages was sustained even when the nonadherent cells were removed from the cultures, and the effect continued for at least 72 hr thereafter. Fractionation of syphilitic nonadherent cell populations by two independent methods produced T cell-enriched preparations with significantly more suppressive activity than non-T cell-enriched preparations. These observations may account for the chronicity of syphilitic infection.
D000328:Adult; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003179:Complement C3b / Q000502:physiology*; D006224:Cricetinae; D006801:Humans; D007111:Immunity, Cellular; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000502:physiology; D008297:Male; D013587:Syphilis / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D003179:Complement C3b