title: Interrelationships in trace-element metabolism in metal toxicities in nickel-resistant strains of Neurospora crassa.
abstract: Three different Ni2+-resistant strains of Neurospora crassa (NiR1, NiR2 and NiR3) have been isolated. All are stable mutants and are fourfold more resistant to Ni2+ than the parent wild-type strain. NiR1 and NiR2 are also sixfold more resistant to Co2+, whereas NiR3 is only twice as resistant to Co2+; the former two are also twofold more resistant to Zn2+, but NiR3 is not. These three strains also differ in sensitivity to Cu2+. Toxicities and concomitant accumulation patterns of Ni2+, Co2+ and Cu2+ have been examined in these strains. NiR1 and NiR2, despite quantitative individual differences, generally accumulate very high amounts of Ni2+ and Co2+, and Mg2+ reverses the toxicities of these two ions by different mechanisms; Ni2+ uptake is suppressed, but not that of Co2+. In NiR3, Mg2+ controls uptake of both Ni2+ and Co2+. Studies indicate that two kinds of Ni2+-resistant strains of N. crassa exist; one kind is resistant because it can tolerate high intracellular concentrations of heavy-metal ions, whereas the other is resistant because it can control metal-ion accumulation.
mesh_terms: D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004351:Drug Resistance; D008274:Magnesium / Q000494:pharmacology; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000187:drug effects / Q000378:metabolism*; D009532:Nickel / Q000378:metabolism* / Q000494:pharmacology; D014131:Trace Elements / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
chemical_list: D014131:Trace Elements; D003035:Cobalt; D003300:Copper; D009532:Nickel; D008274:Magnesium; D015032:Zinc