Studies on the in vitro incorporation of radioactivity from 1-14C-labeled D-hexosamines in the mucopolysaccharide-peptide complexes of avian tissues.
The incorporation of D-[1-14C]-labeled glucosamine (GlcN) and D-[1-14C]-labeled galactosamine (GalN) into mucopolysaccharide-peptide complex(es) (MPS-P) and the rate of 14CO2 production by tissue slices of skin, comb, liver, kidney, shell gland, and magnum from laying hens were studied during a 12 hr period. The D-[1-14C] GlcN was metabolized at a faster rate than D-[1-14C] GalN. No 14CO2 was produced by skin and comb tissues incubated with D-[1-14C]GalN for 12 hr. The amount of 14C associated with the acetone extract of the tissues, acetone-extracted tissues, and MPS-P of the tissue increased with increasing incubation time, but generally the increase was highest in the MPS-P. A comparison among the tissues indicated that the radioactivity present in CO2 and MPS-P was highest in the shell gland and lowest in comb tissue slices. The rates of incorporation of 14C-hexosamine (HexN) into MPS-P by tissue slices appeared to be in general agreement to those of intact animals.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D003129:Comb and Wattles / Q000378:metabolism; D046508:Culture Techniques; D005260:Female; D005688:Galactosamine / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006595:Hexosamines / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010057:Oviducts / Q000378:metabolism; D010452:Peptide Biosynthesis; D012867:Skin / Q000378:metabolism
D006025:Glycosaminoglycans; D006595:Hexosamines; D002245:Carbon Dioxide; D005688:Galactosamine; D005944:Glucosamine