[Nature of the changes in the activity of water-soluble enzymes as affected by noxious agents acting on the muscles. I. A study of the extractability of phosphofructokinase from intact and altered muscles].
Binding of phosphofructokinase (PPK) in intact and thermally altered (15 minutes at 38 degrees C) skeletal muscles of frogs (Rana temporaria) the the extractability of PPK from muscles was studied. PPK activity in actomyosin was also studied. Inhibiting effect of electrolytes (KCl, NaCl, CaCl2, MgCl2) on PPK in muscle extract does not allow to use them for the decision of the question of the interprotein interactions of PPK. 5 mM Na2-EDTA extracts additional PPK from homogenates of intact and altered muscles in comparison with PPK extracted in the media without Na2-EDTA (for intact muscles and altered muscles--509 and 729%). Under alteration of muscle, the binding of PPK increases. Among the structural components of muscle which bind PPK, proteins of actomyosin complex have been found.
D000205:Actomyosin / Q000378:metabolism; D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011896:Rana temporaria; D012995:Solubility; D013696:Temperature
D002712:Chlorides; D000205:Actomyosin; D010732:Phosphofructokinase-1
K voprosu o prirode izmeneniĭ aktivnosti vodorastvorimyk fermentov pri deĭstvii na myshtsy povrezhdaiushchikh agentov. I. Uzuchenie izvlekaemosti fosfofruktokinazy iz intaktnykh i alt'terirovannykh myshts.