The role of platelet surface proteins reacting with heterologous antibodies.
Platelets were labeled with surface-labelling agents which identified up to six platelet membrane proteins. Three of these labelled proteins were recognised by a specific anti-platelet membrane serum by immunoprecipitation. Affinity fractionation of platelets with insolubilised IgG obtained from the anti-platelet membrane serum indicated a greater reaction between the antiserum and the platelet surface. Fab fragments from the antiserum inhibited ristocetin-induced aggregation of platelets and partially inhibited collagen-induced aggregation but did not affect ADP aggregation. Crossed immunoelectrophoresis of platelets using the anti-platelet membrane serum showed that the antiserum was specific for platelet membranes.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001791:Blood Platelet Disorders / Q000175:diagnosis; D001792:Blood Platelets / Q000378:metabolism; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D003979:Diazonium Compounds / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007123:Immunoelectrophoresis, Two-Dimensional; D007457:Iodine Radioisotopes; D008565:Membrane Proteins / Q000276:immunology*; D008970:Molecular Weight; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins; D011817:Rabbits; D013425:Sulfanilic Acids / Q000031:analogs & derivatives / Q000378:metabolism
D000906:Antibodies; D003979:Diazonium Compounds; D006023:Glycoproteins; D007106:Immune Sera; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D013425:Sulfanilic Acids; C000469:diazobenzenesulfonic acid