Sequence analysis of fowl plague virus mutant ts47 reveals a nonsense mutation in the NS1 gene.
A mutant of fowl plague virus, ts47, induces the synthesis in infected cells of a truncated NS1 polypeptide at both permissive and restrictive temperatures. Nucleotide sequence analysis of the segment coding for the NS1 polypeptide, segment 8, indicates that this aberration is due to a nonsense mutation. This mutation occurs in the region of the NS1 gene which overlaps with the NS2 gene and there is a corresponding amino acid substitution in the NS2 polypeptide. While it is not clear which polypeptide is responsible for the thermal instability of ts47, the loss of the COOH-terminal 28 amino acid residues from the NS1 polypeptide does not affect replication of the virus at permissive temperatures.
D001483:Base Sequence; D003062:Codon; D005814:Genes, Viral; D009980:Influenza A virus / Q000235:genetics*; D009154:Mutation; D012333:RNA, Messenger; D012367:RNA, Viral / Q000235:genetics*; D013696:Temperature; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000235:genetics*
D003062:Codon; D012333:RNA, Messenger; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins