An efficient exchange method for alkali light chain in myosin subfragment.
The alkali light chain, A2, in subfragment-1 (S-1) was exchanged with A1 added externally in NH4 + -NH3 buffer (pH 9.9). The exchange yield was higher than 80% using only 2-fold molar excess of A1 over S-1 containing A2. The ATPase activities of the exchanged S-1 (A1) were the same as those of untreated S-1 (A1). The method was also applicable to exchanging the alkali light chains in myosin.
D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009132:Muscles / Q000378:metabolism; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits
D000199:Actins; D015879:Myosin Subfragments; D010446:Peptide Fragments; D000251:Adenosine Triphosphatases; D009218:Myosins