Sarcoplasmic reticulum from flounder muscle having improved lipid peroxidative activity.
A procedure for the isolation of sarcoplasmic reticulum from winter flounder (Pseudopleuronectes americanus) resulted in a fraction with a specific activity of lipid peroxidation two to three times that of previous preparations. In addition, good stability of the NADH-dependent lipid peroxidative activity was achieved. There appeared to be minimal contamination of the preparation with lysosomes and mitochondria. The flounder sarcoplasmic reticulum was highly active with respect to ATPase and calcium uptake. The membrane fraction contained 43% lipid and 57% protein; 60% of the lipids were phospholipids. Phosphatidylcholine was the major phospholipid present.
D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002458:Cell Fractionation; D005399:Fishes; D008054:Lipid Peroxides / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D009132:Muscles / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
D008054:Lipid Peroxides; D008055:Lipids; D011506:Proteins; D009243:NAD; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases; D002118:Calcium