[Study of muscular metabolism of ammonia in the posterior limbs of the intact rat].
In order to evaluate the ammonia, glutamine, and alanine muscular uptake and/or release, we have studied the effects of ammonium acetate perfusions, in intact anaesthetized rat hindquarters, by simultaneous measure of blood flow and arterio-venous differences of ammonia, glutamine and alanine concentrations. Increasing doses of ammonium acetate perfusions (1.23-2.46-3.69 nmol/mm/100 g b.w.) result in an increase of ammonia rat hindquarters uptake which is correlated with the amount of ammonium infused and the arterial ammonia levels but without significant release or uptake of glutamine or alanine. However these perfusions induce an arterial hyperglutaminemia and hypoalaninemia likely due to extramuscular metabolic changes in these two amino-acids.
D000085:Acetates / Q000494:pharmacology; D000409:Alanine / Q000097:blood; D000641:Ammonia / Q000097:blood / Q000378:metabolism*; D000818:Animals; D005121:Extremities; D005973:Glutamine / Q000097:blood; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D005973:Glutamine; D000641:Ammonia; D000409:Alanine; C018824:ammonium acetate
Etude du métabolisme musculaire de l'ammoniaque sur le train postérieur de rat intact.