Rapid purification of deoxyribonuclease I using fast protein liquid chromatography.
Deoxyribonuclease I finds extensive application in the fields of both nucleic acid and cell motility research. This paper describes the use of the cationic exchange column MonoQ, marketed by Pharmacia as part of their Fast Protein Liquid Chromatography system, for further purification of the enzyme from commercially available material. Up to 7 mg DNase I of high purity can be obtained in a single separation step taking about 20 min to perform. The quality of the product is documented using 3 independent assay criteria.
D000818:Animals; D002411:Cation Exchange Resins; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000302:isolation & purification*
D002411:Cation Exchange Resins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I