[Immunoenzyme assay of human cartilaginous proteoglycans and their antibodies].
An ELISA has been developed to measure human cartilaginous proteoglycans (PG) and their auto-antibodies. The assay is described step by step: successively the nature of the microtiter wells, the concentration of the PG to be coated, the optimal dilution of the antiserum, the length of various incubations and their respective temperature, are described. Standard curve obtained with purified PG is linear in a logit-log representation. The sensitivity of the assay is 2 ng/tube. Finally, biological fluids-sérum and synovial fluid-show a good parallelism with PG.
D001323:Autoantibodies / Q000032:analysis*; D002358:Cartilage, Articular / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011509:Proteoglycans / Q000032:analysis* / Q000276:immunology
D001323:Autoantibodies; D011509:Proteoglycans
Dosage enzymo-immunologique des protéoglycanes cartilagineux humains et de leurs anticorps.