Inactivation of the sarcoplasmic reticulum calcium-transport-ATPase by Lasolocid in combination with Triton X-100.
The calcium-transport-ATPase of the sarcoplasmic reticulum membranes is irreversibly inactivated by the combined action of Lasolocid and Triton X-100 at concentrations which separately do not interfere with the enzyme's activity. In the presence of Lasolocid the enzyme is most susceptible to inactivation when the Triton X-100 concentration just exceeds its critical micellar concentration, approximately 0.2 mg . ml-1. Lasolocid becomes effective at a concentration of 10 microM and produces rapid inactivation at 100 microM. The enzyme is more rapidly inactivated in the active than in the inactive state.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D003902:Detergents / Q000494:pharmacology*; D007700:Kinetics; D007832:Lasalocid / Q000494:pharmacology*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; D000252:Calcium-Transporting ATPases; D007832:Lasalocid