title: Identification and quantification of dehydroepiandrosterone sulphate in saliva.
abstract: 3 beta-Hydroxy-5-androsten-17-one (dehydroepiandrosterone) sulphate has been separated from an extract of human saliva by ion exchange gel chromatography and identified by high resolution gas chromatography-high resolution mass spectrometry of the tert-butyldimethylsilyl derivative of the neutral steroid obtained by enzymic hydrolysis. Quantitative analyses, employing 7,7-2H-dehydroepiandrosterone sulphate as the internal standard, have indicated concentrations in the saliva of young adult subjects to be generally in the range 0.9-5.7 nmol/l, though concentrations as high as 10.7 nmol/l have been observed.
mesh_terms: D000328:Adult; D002852:Chromatography, Ion Exchange; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000032:analysis; D019314:Dehydroepiandrosterone Sulfate; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D012463:Saliva / Q000032:analysis*
chemical_list: D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate