Induction of transplantation tolerance in guinea pigs by spleen allografts. II. Responses in the mixed leukocyte reaction correlate with the tolerant state.
We have developed a model for the induction of transplantation tolerance in the guinea pig by vascularized spleen allografts. Spleen allografts from strain 13 to strain 2 hosts frequently survived in healthy recipients without clinical GVHD or induced clinical GVHD. (2 x 13)F1 to strain 2 spleen allografts survived indefinitely without inducing GVHD. In contrast, strain 2 spleen allografts were rejected by strain 13 hosts. An excellent correlation was observed between the clinical course and the degree of reactivity to donor strain stimulator cells in the MLR. Animals that had rejected their grafts had normal or enhanced proliferative responses in the MLR. Strain 2 hosts with long-term surviving strain 13 or (2 x 13)F1 grafts had markedly suppressed anti-13 responses. Animals with GVHD had a suppressed MLR toward donor strain stimulator cells with simultaneous reactivity to host strain stimulator cells. Cells capable of suppressing the response of normal host strain cells to donor strain stimulators were present in some long-term surviving animals and may be responsible in part for the maintenance of the tolerant state.
D000818:Animals; D003433:Crosses, Genetic; D006084:Graft Rejection; D006085:Graft Survival; D006087:Graft vs Host Reaction; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007108:Immune Tolerance; D007111:Immunity, Cellular; D007518:Isoantibodies / Q000096:biosynthesis; D007519:Isoantigens / Q000008:administration & dosage; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D013154:Spleen / Q000637:transplantation*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies; D007519:Isoantigens