Functional reactivities of lymphocytes primed in murine graft-versus-host reactions.
Secondary mixed leukocyte culture (MLC) responses of T lymphocytes effecting graft-versus-host (GVH) reactivity have been compared with the response patterns of T lymphocytes primed in vitro. GVH reactions were initiated in donor-recipient combinations selected to involve genetic differences at the entire H-2 complex or individual K/D and I regions. GVH-primed lymphocytes, whether derived from spleen, liver, or lymph nodes, exhibited unexpectedly specific proliferative reactivity against I region-encoded gene products. In combinations involving both K/D plus I region differences, cytotoxic T lymphocytes were generated against the K/D antigens. These response patterns contrasted to those exhibited by MLC-primed T cells. MLC-primed cells elicited proliferative responses against both K/D and I region antigens and revealed a high cross-reactivity among I region gene products of various haplotypes. The different response patterns for in vivo versus in vitro primed cells do not appear to be artifactual but a result of the priming environment.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006087:Graft vs Host Reaction; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens