Binding of actinomycin D to DNA revealed by DNase I footprinting.
We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X not equal to G and Y not equal to C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D003609:Dactinomycin; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D010957:Plasmids; D013379:Substrate Specificity
D003609:Dactinomycin; D004247:DNA; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I