Structural differences between beef-lung heparan sulphates with specific self-associations.
Three, specifically self-associating variants of heparan sulphate (HS2-A, HS3-A, and HS4-A) from beef lung were subjected to (a) deaminative cleavage of bonds between 2-deoxy-2-sulphoaminoglucose and uronic acid and (b) periodate oxidation of glucuronic acid residues in fully N-acetylated block-regions. In addition, the periodate-oxidised and alkali-cleaved chains were re-oxidised with periodate to identify the glucuronic acid residues in the N-sulphate-containing regions. The results showed that HS2-A was distinguished by much longer (GlcA-GlcNAc)n-segments than HS3-A and HS4-A. The latter two species were characterised by the structure of the variously N-acetyl- and N-sulphate-containing regions. In HS3-A, there was a significant contribution from segments composed of both N-acetylated and N-sulphated 2-amino-2-deoxyglucose residues. The N-sulphate-rich regions contained chiefly iduronic acid. In contrast, HS4-A had mixed or alternating arrangements of the two epimeric uronic acids in the N-sulphate-rich regions. These differences may be the basis for specific self-associations between heparan sulphate chains.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D005347:Fibroblasts / Q000032:analysis; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D008168:Lung / Q000032:analysis*; D046911:Macromolecular Substances
D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D046911:Macromolecular Substances