Phage inactivation and DNA strand scission activities of 7-N-(p-hydroxyphenyl)mitomycin C.
A new derivative of mitomycin C (MMC), 7-N-(p-hydroxyphenyl)mitomycin C (M-83), had higher phage inactivation activity against phages phiX174 and PM2 than MMC, and also higher DNA strand scission activity against their single- and double-stranded DNAs. M-83, at one third to one sixth concentration of MMC, showed the same level of phage inactivation and DNA strand scission activities. The mechanism of phage inactivation and DNA strand scission by M-83 were similar to those of MMC: (1) Reduction of M-83 was required for its action. (2) Oxygen radicals were involved in DNA strand scission, and metal ions possibly participated in the generation of oxygen radicals. (3) DNA strand scission was single strand scission, and dependent on temperature. The high DNA strand scission activity of M-83 is considered to reflect the rapid conversion to the active form.
D010584:Bacteriophage phi X 174 / Q000187:drug effects; D001435:Bacteriophages / Q000187:drug effects*; D004279:DNA, Viral / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D011549:Pseudomonas
D004279:DNA, Viral; D008937:Mitomycins; D016685:Mitomycin; C027281:7-N-(4-hydroxyphenyl)mitomycin C