Cytochemical, functional, and proliferative characteristics of promonocytes and monocytes from patients with monocytic leukemia.
This article deals with a prospective study on the cytochemical, functional, and proliferative characteristics of promonocytes and bone marrow and peripheral blood monocytes of 20 patients with acute monocytic leukemia and 7 patients with chronic monocytic leukemia. The results show a wide variation in the peroxidase and esterase activities in these cells, whereas the percentages of mononuclear phagocytes with Fc gamma and C3b receptors did not differ appreciably from those in normal individuals. A discriminant analysis of these data and corresponding data from normal individuals showed that a below-normal peroxidase activity of circulating monocytes has predictive value for the presence of monocytic leukemia; a below-normal esterase activity has less, but nevertheless some, predictive value in this respect. An increase in the percentage of circulating monocytes, a decrease in the percentage of Fc gamma or C3b receptors, and a decline in the ability to phagocytose bacteria has no predictive value for the presence of monocytic leukemia. The mean percentage of patients' promonocytes that incorporated 3H-thymidine amounted to 80.9%, which is close to the control value in normal individuals. The mean values for the labeling indices of cultured bone marrow and peripheral blood monocytes are 1.0% and 0.74%, respectively; when 3H-thymidine was added to whole blood, the labeling index of the monocytes amounted to 3.6%. These percentages are only a little higher than those found for monocytes of normal individuals. These results indicate that the majority of the circulating monocytes in acute and chronic monocytic leukemia are not actively dividing or blast cells.
D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D004950:Esterases / Q000378:metabolism; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000097:blood*; D007951:Leukemia, Myeloid / Q000097:blood*; D009000:Monocytes / Q000201:enzymology / Q000276:immunology*; D010544:Peroxidases / Q000378:metabolism; D010587:Phagocytosis; D010873:Pinocytosis; D011446:Prospective Studies; D011951:Receptors, Complement / Q000378:metabolism; D011961:Receptors, Fc / Q000378:metabolism; D017452:Receptors, IgG; D013210:Staphylococcus / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D011951:Receptors, Complement; D011961:Receptors, Fc; D017452:Receptors, IgG; D010544:Peroxidases; D004950:Esterases; D013936:Thymidine