title: Gel-chromatographic analysis of 99mTc-labeled human serum albumin prepared with Sn(II) as the reductant.
abstract: The labeling of human serum albumin (HSA) with 99mTc using Sn(II) as the reductant has been reinvestigated in view of the possibility of formation of Sn-Tc colloids. Preparations of 99mTc-HSA and mixtures of 99mTc-TcO-4 with Sn(II)Cl2 have been analyzed by means of gel chromatography using various gel matrices and eluents. No Sn-Tc colloids could be eluded from samples of 99mTc-HSA preparations. The percentage of labeling was about 90%. A comparative investigation of a number of gel-chromatographic systems for the detailed analysis of 99mTc-HSA has been made. It is found that a long column with Sephadex G-200 gives the best results.
mesh_terms: D002850:Chromatography, Gel; D007553:Isotope Labeling / Q000379:methods; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000032:analysis*; D013667:Technetium / Q000032:analysis*; D017556:Technetium Compounds; D013668:Technetium Tc 99m Aggregated Albumin; D014001:Tin / Q000032:analysis; D017971:Tin Compounds
chemical_list: D012709:Serum Albumin; D017556:Technetium Compounds; D013668:Technetium Tc 99m Aggregated Albumin; D017971:Tin Compounds; C033493:technetium Tc 99m tin colloid; D013667:Technetium; D014001:Tin