A radioisotope method for assays of amylomaltase and D-enzyme.
A method has been developed for the assay of amylomaltase based on the incorporation of a [14C]glucose moiety of uniformly 14C-labeled maltose into a maltodextrin fraction insoluble in aqueous ethanol. The presence of dextrin at a high concentration greatly enhances [14C]glucose incorporation and serves to minimize interference with the assay by contaminating enzymes that hydrolyze substrates and products in the assay mixture. Since a number of other enzymes are capable of forming glucose in the assay mixture, the 14C incorporation is a more specific method of enzyme assay than are previously reported assays based on glucose release.
D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000032:analysis*; D006004:Glycogen Debranching Enzyme System; D007700:Kinetics; D008320:Maltose / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013295:Streptococcus mutans / Q000201:enzymology
D002250:Carbon Radioisotopes; D006004:Glycogen Debranching Enzyme System; D011134:Polysaccharides; D008320:Maltose; C008315:maltodextrin; D005964:Glucosyltransferases; C020569:4 alpha-glucanotransferase; D005947:Glucose