[Cytoskeletal reorganization in hepatocytes of the regenerating mouse liver].
The intracellular pattern of prekeratin and actin filaments has been studied on sections of mouse livers regenerating after CCl4 injury. Monoclonal antibodies against one of liver prekeratins and monospecific polyclonal actin antibodies were used in the indirect immunofluorescent test. The presence of alpha-fetoprotein and bile canaliculi antigen was also monitored during regeneration. In control livers, prekeratin and actin filaments formed thick bundles adjacent to plasma membranes. The cytoplasmic prekeratin network was unmarked. In contrast to the latter, the bright well developed intracytoplasmic prekeratin network and intracytoplasmic actin fibers were identified in the perinecrotic hepatocytes by the 3d-4th day of regeneration. This rearrangement of the cytoskeleton coincided in time with the appearance of alpha-fetoprotein and the loss of the bile canaliculi antigen in the perinecrotic hepatocytes.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism / Q000473:pathology; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000509:alpha-Fetoproteins
Perestroĭka tsitoskeleta v gepatotsitakh regeneriruiushcheĭ pecheni mysheĭ.