Keratan sulfate proteoglycan isolated from the estrogen-induced medullary bone in Japanese quail.
1. Two proteoglycans isolated from the femurs of quail actively producing medullary bone were separated using DEAE Bio-Gel A. 2. The first to elute in the gradient was a keratan sulfate proteoglycan with an average buoyant density of 1.53 g/ml and a Kav = 0.57 on Sepharose CL-4B. 3. The second proteoglycan to elute contained chondroitin 4-sulfate. 4. Apparently only the keratan sulfate proteoglycan is associated with the new medullary bone matrix.
D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D003370:Coturnix; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005269:Femur / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D007632:Keratan Sulfate / Q000096:biosynthesis* / Q000302:isolation & purification; D000071234:Lumican; D008297:Male; D011509:Proteoglycans / Q000096:biosynthesis*
D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D000071234:Lumican; D011509:Proteoglycans; D004958:Estradiol; D007632:Keratan Sulfate