Fluorescence study of secondary structure of DNA within bacteriophage lambda.
Bromoacetaldehyde (BAA) was used to study the secondary structure of DNA in lambda-phage particles. It was determined that about 1% of the adenines in the intraphage lambda-DNA reacts readily with BAA, thus, they are placed in DNA sites with disturbed complementary interactions. These adenines are close to the tryptophan residues of the phage protein. Fluorescence emission of epsilon A in the intraphage DNA is dramatically quenched. This, apparently, indicates the interaction between epsilon A and Trp- and/or Tyr- and/or Met-residues of phage protein.
D010582:Bacteriophage lambda / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004279:DNA, Viral / Q000302:isolation & purification*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence
D004279:DNA, Viral