title: Genetic analysis of murine arylsulfatase C and steroid sulfatase.
abstract: SWR/J mice possess two- to threefold higher 4-methylumbelliferyl sulfate (4MUS), dehydroepiandrosterone sulfate (DHEAS) and estrone sulfate (E1S) sulfatase activities in liver and kidney extracts than do A/J mice. These interstrain activity differences are maintained throughout the 6- to 45-day postnatal period. Characteristics of the hepatic activities of SWR/J mice suggest that all three activities reside in the same enzyme. Biochemical properties of the SWR/J and A/J enzyme were not significantly different. Expression of hepatic enzyme activity is subject to regulation by an autosomal locus possessing two alleles with additive effects. Postnuclear E1S- and DHEAS-sulfatase activities are primarily microsomal. Although postnuclear hepatic 4MUS-sulfatase activity is predominantly microsomal, renal activity is primarily nonmicrosomal. Only that portion of 4MUS-sulfatase occurring in cell membranes appears capable of hydrolyzing E1S and DHEAS. The hepatic- and renal-specific subcellular distributions of 4MUS-sulfatase activity may reflect tissue differences in enzyme processing. Renal 4MUS-sulfatase activity is also controlled by an autosomal gene with two alleles having additive effects. Positive correlation between hepatic and renal 4MUS-sulfatase activities indicates that both activities are most likely influenced by the same gene.
mesh_terms: D000483:Alleles; D000818:Animals; D001192:Arylsulfatases / Q000235:genetics*; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000378:metabolism; D019314:Dehydroepiandrosterone Sulfate; D004970:Estrone / Q000031:analogs & derivatives / Q000378:metabolism; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008862:Microsomes, Liver / Q000201:enzymology; D043266:Steryl-Sulfatase; D013379:Substrate Specificity; D013429:Sulfatases / Q000235:genetics*
chemical_list: C019492:4-methylumbelliferyl sulfate; D004970:Estrone; D006923:Hymecromone; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D013429:Sulfatases; D001192:Arylsulfatases; D043266:Steryl-Sulfatase; C017296:estrone sulfate