Isolation and sequence determination of two pyridoxal 5'-phosphate-labeled thermolysin peptides from pig muscle phosphoglucose isomerase.
Pig muscle phosphoglucose isomerase modified with pyridoxal 5'-phosphate under conditions that cause at least 90% inactivation of its catalytic activity was found to incorporate about 1.5 eq of pyridoxal 5'-phosphate per subunit. After digestion with thermolysin, two pyridoxal 5'-phosphate-containing peptides were isolated and their amino acid sequences were determined to be Leu-Gly-pyridoxyl-Lys-Gln and Ile-Ala-Ser-pyridoxyl-Lys-Thr.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D010733:Phosphoglucomutase / Q000302:isolation & purification*; D011485:Protein Binding; D011732:Pyridoxal Phosphate / Q000032:analysis*; D013552:Swine; D013820:Thermolysin
D000596:Amino Acids; D010446:Peptide Fragments; D011732:Pyridoxal Phosphate; D013820:Thermolysin; D010733:Phosphoglucomutase