Neutral glycosphingolipids of murine myeloma cells and helper, cytolytic, and suppressor T lymphocytes.
Functionally defined clones and lines of murine lymphocytes including myelomas, helper, suppressor and cytolytic T lymphocytes were analyzed for their glycosphingolipids (GSLs). GSLs were characterized by thin-layer chromatography and by high-performance liquid chromatography. Lymphocytes with different functions displayed, besides a number of common GSLs, some characteristic GSLs that may be regarded as markers. Globotriaosylceramide was found on myelomas and B blasts, whereas globotetraosylceramide was confined to helper T cells. All T cells including cytolytic T lymphocytes displayed gangliotetraosylceramide (asialo-GM1) as a major GSL, which was further characterized by sequential degradation with exoglycosidases.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000032:analysis; D005677:G(M1) Ganglioside; D005915:Globosides / Q000032:analysis; D006028:Glycosphingolipids / Q000032:analysis*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009101:Multiple Myeloma / Q000032:analysis*; D013602:T-Lymphocytes, Cytotoxic / Q000032:analysis*; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis*; D050378:T-Lymphocytes, Regulatory / Q000032:analysis*; D014281:Trihexosylceramides
D005915:Globosides; D006028:Glycosphingolipids; D014281:Trihexosylceramides; C024032:globotetraosylceramide; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; C018549:globotriaosylceramide