Fibrinogen Bondy: a new case of dysfibrinogenemia. Isolation of the abnormal fibrinogen molecules.
In a 81 year old health woman, gross abnormalities of fibrin formation led to the discovery of an abnormal fibrinogen named fibrinogen Bondy. Clottability of purified fibrinogen Bondy was only 53% compared to 95-98% for normal fibrinogen. Functional studies revealed (i) delayed coagulation by thrombin and batroxobin (Reptilase), (ii) incomplete release of fibrino-peptides A and B, (iii) poor fibrin monomer aggregation, (iv) delayed fibrin proteolysis by plasmin. Electrophoretic mobility of fibrinogen Bondy, its three chains and the products of fibrin cross-linking, was normal. Fibrinogen NH2-terminal residues of fibrinogen Bondy were found to be normal. The presence of Ala, in addition to Gly and Tyr in the fibrin clot and its supernatant, showed that a part of fibrinogen molecules was not clotted, i.e. either copolymerised with fibrin or remaining in solutions. Gel filtration of the supernatant allowed the separation of both soluble complexes and fibrinogen. This fibrinogen population was shown to be unclottable by thrombin and to inhibit clotting of normal fibrinogen.
D000368:Aged; D001778:Blood Coagulation Disorders / Q000097:blood* / Q000175:diagnosis / Q000209:etiology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D015241:Fibrinogens, Abnormal; D005341:Fibrinolysin / Q000494:pharmacology; D005344:Fibrinopeptide A / Q000378:metabolism; D005345:Fibrinopeptide B / Q000378:metabolism; D006801:Humans; D013918:Thrombin Time
D003432:Cross-Linking Reagents; D005338:Fibrin Fibrinogen Degradation Products; D015241:Fibrinogens, Abnormal; C011468:fibrinmonomer; C036357:fibrinogen Bondy; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D005340:Fibrinogen; D005341:Fibrinolysin