title: Ribosomal genes of Neurospora crassa: constancy of gene number in the conidial and mycelial phases, and homogeneity in length and restriction enzyme cleavage sites within strains.
abstract: We report the results of experiments which, while not specifically designed to study the possibility of rDNA amplification during different developmental stages in the N. crassa life cycle, clearly indicate a relative constancy in the rDNA content of conidia (asexual spores) and mycelial cells. We also report the results of restriction enzyme studies which indicate that the Neurospora rDNA repeat units are homogeneous in length and restriction site pattern within any given Neurospora strain. These results directly contradict the recent report of Dutta et al. (1983), in which the authors concluded that the rDNA of germinating conidia is amplified, relative to mycelia, and that up to 10% of the rDNA units are heterogeneous.
mesh_terms: D004247:DNA / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal; D005784:Gene Amplification; D005800:Genes, Fungal; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D004247:DNA