Phorbol ester-induced changes in human leukemic pre-B lines.
The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin mu chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin mu chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant mu chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged mu chain genes.
D000951:Antigens, Neoplasm / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D007938:Leukemia / Q000235:genetics / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology; D015260:Neprilysin; D010704:Phorbols / Q000494:pharmacology*; D013234:Stem Cells / Q000276:immunology / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000951:Antigens, Neoplasm; D007148:Immunoglobulin mu-Chains; D010704:Phorbols; D015260:Neprilysin; D013755:Tetradecanoylphorbol Acetate