Depletion of T-lymphocyte subsets using monoclonal antibody and complement: effect on T-colony formation.
Using monoclonal antibody and fresh rabbit serum, mononuclear cells from 11 healthy individuals were depleted of either helper or suppressor T-lymphocytes by complement-mediated lysis. The effect on T-lymphocyte colony formation was studied to determine the nature of the T-colony-forming cell. While depletion of either subset of T-lymphocytes significantly reduced T-colony formation compared to controls (P less than 0.01), colony formation did not differ significantly between helper or suppressor T-cell-depleted cells. Thus T-colony formation appears to be a property of both helper and suppressor T-lymphocytes, and both subsets are necessary for optimal colony formation.
D000911:Antibodies, Monoclonal; D002999:Clone Cells / Q000166:cytology; D003165:Complement System Proteins; D006801:Humans; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology
D000911:Antibodies, Monoclonal; D003165:Complement System Proteins