Streptozotocin, but not alloxan, induces DNA repair synthesis in mouse pancreatic islets in vitro.
In the present investigation, the abilities of streptozotocin and alloxan to induce DNA repair synthesis in isolated mouse pancreatic islets have been compared using an autoradiographic technique. Streptozotocin exposure in vitro induced a dose-dependent DNA repair synthesis, whereas no such effect was observed after alloxan treatment. The hydroxyl radical scavenger dimethyl urea and the poly(ADP-ribose) synthetase inhibitors nicotinamide and theophylline reduced the streptozotocin-induced DNA repair. The results suggest that the initial events in streptozotocin-induced B cell injury are DNA damage and repair and that alloxan exerts its major cytotoxic effect by a different mechanism.
D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008782:Methylurea Compounds / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009536:Niacinamide / Q000494:pharmacology; D013311:Streptozocin / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology
D008782:Methylurea Compounds; D009536:Niacinamide; D013311:Streptozocin; D000496:Alloxan; D013806:Theophylline