Effects of some clinically significant mycotoxins on the incorporation of DNA, RNA and protein precursors in cultured mammalian cells.
The effects of zearalenone (F-2), ochratoxin A and T-2 toxins on the synthesis of DNA, RNA and proteins in mouse L cells were studied. F-2 toxin inhibited protein synthesis to a lesser extent than DNA and RNA synthesis, whereas ochratoxin A inhibited the synthesis of each equally. Exposure to the toxins for 24 hours relatively reduced the synthetic ability of the cells. T-2 toxin inhibited protein and DNA synthesis in parallel but RNA synthesis to a lesser extent. Enhanced incorporation of tritiated thymidine was found when L cells were exposed to 0.4 to 0.016 ng of T-2 toxin ml-1 for 24 hours.
D000818:Animals; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D007822:Laryngeal Neoplasms; D007930:Leucine / Q000378:metabolism*; D051379:Mice; D009183:Mycotoxins / Q000494:pharmacology*; D009793:Ochratoxins / Q000494:pharmacology; D013605:T-2 Toxin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism*; D015025:Zearalenone / Q000494:pharmacology
D009183:Mycotoxins; D009793:Ochratoxins; D014316:Tritium; C025589:ochratoxin A; D015025:Zearalenone; D007930:Leucine; D013605:T-2 Toxin; D013936:Thymidine; D014529:Uridine