Partial purification and characterization of an endogenous granulomonopoiesis inhibitor from calf spleen.
A factor which specifically inhibits the proliferation of murine bone-marrow-cell colonies in vitro was extracted from calf spleen and partially purified. No comparable inhibition in T-lymphocyte or erythrocyte assays was observed, suggesting a possible chalone nature of the factor. The formation of granulocyte and macrophage colonies is inhibited to equal degrees. The factor was found to be non-toxic: Bone-marrow cells exposed to the factor for 5 h proliferated at a normal rate thereafter. Solubility, molecular mass and ionic exchange data of the factor are similar to those found for chalone-like inhibitors derived from other sources. The present inhibitor however does not contain a thiol group, and has no stimulatory effect after oxidation as described previously for similar factors.
D000818:Animals; D001854:Bone Marrow Cells; D002417:Cattle; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000502:physiology*; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000502:physiology; D006410:Hematopoiesis; D011506:Proteins / Q000032:analysis; D013154:Spleen / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000032:analysis; D014462:Ultrafiltration
D006131:Growth Inhibitors; D011506:Proteins; D013438:Sulfhydryl Compounds