title: On the fidelity of DNA replication. Lack of primer position effect on the fidelity of mammalian DNA polymerases.
abstract: Mechanisms for the fidelity of DNA replication in eucaryotes are not adequately understood. Certain hypotheses can be tested by examining whether the first nucleotide inserted is incorporated with a significantly higher error rate than subsequent nucleotides. Using synthetic oligodeoxynucleotides, we have measured the effect of primer position on single-base misinsertion frequencies at an amber site in phi X174 DNA. Our results show a lack of position effect, indicating that processivity and the most direct "energy relay" proofreading mechanisms are not important determinants in eucaryotic replication fidelity.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics; D004256:DNA Polymerase I / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D008345:Manganese / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D008345:Manganese; D004256:DNA Polymerase I; D004257:DNA Polymerase II