T cell and T cell subset determination in normal peripheral blood: comparison of the indirect immunofluorescence and lymphocytotoxicity techniques.
We compared a simple complement-dependent lymphocytotoxicity test with a widely used indirect immunofluorescence procedure to enumerate total T, T helper, and T suppressor lymphocytes in normal blood samples. Results with the two techniques were closely similar.
D000328:Adult; D000911:Antibodies, Monoclonal; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology
D000911:Antibodies, Monoclonal