Analysis of chromatin of the brain of young and old rats by micrococcal nuclease and DNase I.
Micrococcal nuclease (MCN) and DNase I were used to study the conformational changes in chromatin of the brain of rats of different ages. Purified nuclei and chromatin were digested separately by MCN and DNase I. Kinetics of digestion of chromatin by MCN are similar for young, adult and old rats. Also agarose gel electrophoresis of DNA fragments do not show any differences. The kinetics of digestion with DNase I, on the other hand, are greater and faster for 20-week old rats than for 90-week old rats. High performance denaturing polyacrylamide gel electrophoresis reveals that a greater amount of smaller fragments of DNA are produced in the 20-week old rats than in the 90-week. These conformational changes occur in the chromatin during aging.
D000375:Aging; D000818:Animals; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000032:analysis*; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004706:Endodeoxyribonucleases; D005260:Female; D007700:Kinetics; D008836:Micrococcal Nuclease; D011487:Protein Conformation; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease