Analytical and functional studies on the T cells of untreated and immunologically tolerant diabetes-prone BB rats.
Lymphocyte profiles of untreated diabetes-prone BB rats (BBDP) and their normal marrow-inoculated tolerant littermates were determined with the aid of a fluorescence-activated cell sorter by using monoclonal antibodies specific for rat T cell (W3/13) and T cell subsets (W3/25, T helper; OX8, T cytotoxic/suppressor). In contrast to non-diabetes-prone rats and to their marrow-inoculated littermates, untreated BBDP rats were found to be T lymphocytopenic with reduced W3/25+ cells and with a major loss of OX8+ cells. Moreover, their lymphocytes displayed depressed alloreactivity that was not restored by addition of exogenous IL 2.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D016026:Bone Marrow Transplantation; D003921:Diabetes Mellitus, Experimental / Q000276:immunology* / Q000628:therapy; D005260:Female; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000502:physiology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D010641:Phenotype; D051381:Rats; D011912:Rats, Inbred ACI; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D011920:Rats, Inbred WF; D013601:T-Lymphocytes / Q000276:immunology*; D013897:Thoracic Duct / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2