A high affinity Ca2+-ATPase in enriched nerve-ending plasma membranes.
A preparation of enriched synaptosomal plasma membrane (SPM) from mouse brain was found to contain a Mg2+-independent high affinity Ca2+-activated ATPase. The preparation readily accumulated Ca2+ at micromolar concentration from the medium in the presence or absence of Mg2+ and released previously accumulated calcium upon the addition of ATP. It is concluded that SPM Ca2+-ATPase may have a functional role in removal of cytosol Ca2+.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D013574:Synaptosomes / Q000201:enzymology
D000252:Calcium-Transporting ATPases; D008274:Magnesium