title: Distribution of actin content in human B and T lymphocytes by DNAse 1 inhibition test.
abstract: The DNAse inhibition assay, which allows for the titration of two forms of soluble actin in cell extracts, has been applied to normal human lymphocytes from peripheral blood. Actin-like activities associated with the membrane-rich fraction of extracts from thymus dependent (T) lymphocytes are significantly higher than those from bone marrow derived (B) lymphocytes, on a per cell basis, provided care is paid to the elimination of monocytic and polymorphonuclear cells which produce significantly higher DNAse 1 inhibition. The apparent discrepancy with a recent report that normal T and B lymphocytes do not differ in their total actin content is discussed with respect to the functional uniqueness of membrane-associated DNAse 1 inhibitions and the importance of using purified lymphocyte populations.
mesh_terms: D000199:Actins / Q000097:blood*; D001402:B-Lymphocytes / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors*; D006801:Humans; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000199:Actins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I