Purification of the bacteriophage lambda late gene regulator encoded by gene Q.
The product of bacteriophage lambda gene Q is a transcription antiterminator that activates phage late gene expression. We report a method to purify the lambda Q protein to near homogeneity. We have followed during purification both activity of Q protein to provoke in vitro synthesis of the lambda late protein endolysin in the DNA-dependent protein-synthesizing system, and radioactivity in a polypeptide that we show by genetic criteria to be lambda Q. These co-purify throughout the procedure.
D010582:Bacteriophage lambda / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000096:biosynthesis; D005814:Genes, Viral; D014764:Viral Proteins / Q000302:isolation & purification*
D014764:Viral Proteins; D010450:Endopeptidases; C001143:endolysin