[Liver transplantation. Experimental aspects].
Following an overview of the data published in the specialized literature in connection with liver transplantation the author presents original experimental studies in this field. One of the first aspects considered is the stage of obtaining the graft of liver tissue. The selective perfusion was used, in situ, of the dog liver, and conditions were achieved which were similar to those obtained in other methods of graft preparation. Two washing solutions were used: a simple one, currently used in the practice, and another one which was enriched with various substrates. Biochemical parameters of tissue sampled by bioptic puncture have demonstrated that the first solution induces a lowering of the glycogen contents of hepatocytes, and this alters the biological qualities or the graft. The fact is stressed that the obtention of the hepatic tissue for grafting should be considered in fact as an in situ conservation. With regard to the liver transplantation proper it is shown that the author has performed the sector heterotopic procedure in the rat. Vascular anastomoses have been made with histoacryl-N-blau by the method of prosthesis with lost tubing. Problems raised by the vascular re-connection of the auxilliary hepatic tissue, and those related to the space where this tissue should be placed are also discussed in detail. The survival time was not longer than 30 hours.
D000818:Animals; D004285:Dogs; D016031:Liver Transplantation; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous
Transplantul hepatic - aspecte experimentale.