title: [The type of covalent bond in a natural complex of the bacteriophage phi X 174 protein A* and nucleotides].
abstract: The 32P-labelled A* protein has been isolated from E. coli cells infected by phage phi X174 in the presence of [32P]orthophosphate. The snake venom phosphodiesterase treatment of the [32P]peptides obtained by the pronase digestion of the protein has revealed a phosphodiester bond between the protein and a nucleotide material of A, G base composition. The hydrolysis of nucleotide-peptides with a mixture of concentrated HCl and CF3COOH has yielded 4'O-phosphotyrosine.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000032:analysis / Q000235:genetics*; D002848:Chromatography, DEAE-Cellulose; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D009711:Nucleotides / Q000032:analysis* / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D004279:DNA, Viral; D009711:Nucleotides; D014764:Viral Proteins
vernacular_title: Izuchenie tipa kovalentnoĭ sviazi v prirodnom soedineniĭ belka A* bakteriofaga phi X 174 s nukleotidami.