Evidence for the presence of DNase-actin complex in L1210 leukemia cells.
L1210 leukemia cell cytosol was analysed for the presence of DNase I activity. No free activity was determined in crude cytosol. DNase I enzyme was found to occur in a latent form bound to cytoplasmic actin. DNase-actin complex was partially isolated by Sephadex filtration and DNase I-like activity was demonstrated after SDS gel electrophoresis of the complex and enzyme renaturation. The results were compared with those for synthetic complex of pancreatic bovine DNase I and chicken muscle actin.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice; D008811:Mice, Inbred DBA
D000199:Actins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I