[Effect of temperature on fluorescence of labels and probes of various localizations incorporated into sarcoplasmic reticulum samples].
Studies were carried out of temperature relationship of dansylchloride, N-3-pyrenylmaleinimide fluorescence, SR membranes, self-luminescence caused by Ca-ATPase tryptophane - provided fluorescence and of pyrene excimerization in membrane preparations of sarcoplasmic reticulum (SR) of rabbit skeletal muscles. Temperature relationship of fluorescence intensity of dansylchloride and N-3-pyrenylmaleimide in Arrhenius coordinates has bends at 15 and 35 degrees. Selffluorescence of protein samples linearly depends on temperature. Temperature relationship of the ratio between the intensities of exsimeric and monomeric forms of pyrene Fa/Fm in Arrhenius coordinates has the bend at 20-22 degrees. Hence only the latter relationship coincides with the shape of Arrhenius graph for enzymatic activity of SR Ca-ATPase.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis; D005456:Fluorescent Dyes; D009132:Muscles / Q000032:analysis* / Q000201:enzymology; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000032:analysis* / Q000201:enzymology; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics
D005456:Fluorescent Dyes; D000252:Calcium-Transporting ATPases
Vliianie temperatury na fluorestsentsiiu metok i zondov razlichnoĭ lokalizatsii, vstroennykh v preparaty sarkoplazmaticheskogo retikuluma.