title: Levels of filamentous and globular actin in Chinese hamster ovary cells throughout the cell cycle.
abstract: Synchronous Chinese hamster ovary (CHO) cells were obtained by mitotic selection and the levels of globular (G) actin, filamentous (F) actin, and cytoskeletal-associated F-actin were determined as cells progressed through the cell cycle. Total actin levels remained quite constant when expressed as a percent of the total protein. An increase in F-actin occurred upon plating the mitotic cells, but this increase was shown to be a result of attachment to the substratum, since cells which remained attached during the second mitosis failed to show these changes. No large variation in the levels of either F-actin or cytoskeletal-associated F-actin occurred throughout the cell cycle. Therefore, changes in the morphology of the CHO cells which are accompanied by a reorganization of actin-containing microfilaments during the cell cycle are not accompanied by significant changes in the size of the monomeric actin pool.
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors; D005260:Female; D010053:Ovary / Q000032:analysis / Q000166:cytology*
chemical_list: D000199:Actins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I