Plaque-forming cells in man. II. Evidence of the existence of active suppressor cells in peripheral blood of normal plaque-forming cell non-responders.
When the immunoglobulin secretion of 172 normal healthy individuals was investigated with the protein-A plaque assay, 12 persons (7%) did not develop any plaque-forming cells (PFC) in cultures of pokeweed mitogen (PWM)-activated unfractionated peripheral blood lymphocytes (PBL), incubated for 6 days. The effect of irradiation on normal PFC responders and non-responders was also investigated; 2000-rad-irradiated non-responder T lymphocytes co-cultured with autologous untreated B lymphocytes restored the PFC response to normal levels. The evidence of a high level of suppressor activity in non-responder T lymphocytes was further demonstrated by the decreased PFC response of normal B lymphocytes co-cultured with untreated non-responder T lymphocytes.
D000328:Adult; D000921:Antibody-Producing Cells / Q000276:immunology* / Q000528:radiation effects; D001402:B-Lymphocytes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006462:Hemolytic Plaque Technique; D006801:Humans; D007108:Immune Tolerance; D007136:Immunoglobulins / Q000096:biosynthesis; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects
D007136:Immunoglobulins