title: Phylogeny of interleukins: growth factors produced by leucocytes of the cyprinid fish, Cyprinus carpio L.
abstract: Supernatants of phytohaemagglutinin (PHA)-activated pronephric leucocytes from carp (Cyprinus carpio L.) contain a lymphocyte growth factor which can induce a proliferative response of purified lymphoblasts but not freshly isolated leucocytes. The growth-promoting activity can be reduced by absorbing the supernatant with mitogen-activated blasts. In addition, increased incorporation of tritiated thymidine into PHA-activated blast cells is also induced by supernatants from two-way mixed leucocyte cultures. The data show that even at the evolutionary level of teleost fish, amplifying/regulatory leucocyte products exist. It is suggested that these factors play as important a role in the regulation of the immune response in fish as they do in mammals.
mesh_terms: D000818:Animals; D002347:Carps / Q000276:immunology*; D002478:Cells, Cultured; D003470:Culture Media; D003530:Cyprinidae / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D010802:Phylogeny; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007376:Interleukin-2; D010835:Phytohemagglutinins