title: Variation in accessory cell requirements in human mixed lymphocyte response to leukaemic cell lines.
abstract: Human leukaemia and lymphoma cell lines were investigated as stimulating cells in an allogeneic mixed lymphocyte response. Purified T cells and unfractionated mononuclear cells from normal donors were used as responders. The cell lines fell into three groups: (i) those which stimulated allogeneic responder T cells in the presence or absence of accessory (non-T) cells; (ii) those which stimulated T cells only in the presence of accessory cells; and (iii) those which failed to stimulate in either case. The accessory function was provided by adherent cells and non-adherent, non-T cells. There was no correlation between the stimulatory capacity of these cell lines and the presence of serologically defined HLA-DR determinants. These results are discussed in the context of the current two-signal hypothesis for T-cell activation.
mesh_terms: D000328:Adult; D002460:Cell Line; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007938:Leukemia / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007213:Indomethacin