Semliki Forest virus penetration from endosomes: a morphological study.
The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to synchronize endosomal acidification, and 20 degrees C incubation was employed to concentrate virus particles into the endosomal compartment.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D008854:Microscopy, Electron / Q000379:methods; D011991:Receptors, Virus / Q000502:physiology*; D012672:Semliki forest virus / Q000235:genetics* / Q000648:ultrastructure
D011991:Receptors, Virus