Macromolecular synthesis is required for stimulation of estrogen synthetase activity by dibutyryl cyclic AMP plus theophylline in choriocarcinoma cell culture.
Estrogen secretion and estrogen synthetase (aromatase) activity are stimulated in human trophoblast cells (JAr line) after addition of 1 mM dibutyryl cyclic AMP plus 1 mM theophylline (dbT) to the growth medium. The data given here show that (a) the aromatase specific activity in homogenized cells increases linearly over a 96 hr incubation period after addition of dbT; (b) addition of inhibitors of macromolecular synthesis, cycloheximide or actinomycin D, to the culture medium at the time of addition of dbT abolishes the stimulation of aromatase activity; (c) mixing dbT-grown cells, containing increased aromatase activity, with control cells does not result in an aromatase specific activity higher than the expected average, suggesting that dbT-grown cells do not contain a factor present in excess which serves to stimulate aromatase in control cells; and (d) NADPH, included in vitro in the aromatase assay or incubated with the cells for 48 hr as well as being present in the aromatase assay, has no stimulatory effect on aromatase specific activity in homogenized cells.
D001141:Aromatase / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000201:enzymology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006801:Humans; D046911:Macromolecular Substances / Q000096:biosynthesis*; D009249:NADP / Q000502:physiology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D011247:Pregnancy; D013806:Theophylline / Q000494:pharmacology*; D014594:Uterine Neoplasms / Q000201:enzymology*
D004967:Estrogens; D046911:Macromolecular Substances; D003609:Dactinomycin; D009249:NADP; D003994:Bucladesine; D003513:Cycloheximide; D013806:Theophylline; D010088:Oxidoreductases; D001141:Aromatase; D009251:NADPH-Ferrihemoprotein Reductase