Regulation of gonadotropin receptors and gonadotropin responses in a clonal strain of Leydig tumor cells by epidermal growth factor.
The MA-10 line is a clonal strain of Leydig tumor cells that has receptors for human choriogonadotropin (hCG) and mouse epidermal growth factor (mEGF). These cells respond to hCG, cholera toxin, and 8-Br-adenosine 3':5'-monophosphate with increased steroid production. It is reported herein that exposure of the MA-10 cells to mEGF results in a substantial (80 to 90%) reduction in the number of hCG receptors per cell. The loss of hCG receptors is accompanied by a corresponding reduction in the ability of hCG to stimulate steroidogenesis. The steroidogenic responses to cholera toxin and 8-Br-adenosine 3':5'-monophosphate, however, are not affected. Other results presented show that mEGF is not a mitogen for these cells.
D004092:20-alpha-Dihydroprogesterone / Q000096:biosynthesis; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000818:Animals; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D002999:Clone Cells; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007700:Kinetics; D007984:Leydig Cell Tumor / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011974:Receptors, LH
D006063:Chorionic Gonadotropin; D010455:Peptides; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D004092:20-alpha-Dihydroprogesterone; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011374:Progesterone; D004815:Epidermal Growth Factor; D002772:Cholera Toxin; D000242:Cyclic AMP