Tissue-specific exposure of chromatin structure at the 5' terminus of the rat preproinsulin II gene.
The 5' terminus of the rat preproinsulin II gene exhibits a tissue-dependent DNase I sensitivity. Only in the chromatin froma pancreatic beta-cell tumor, but not in liver, spleen, kidney, or brain chromatin, is a region at and before the 5' end of the gene exposed to cleavage. The region of exposure extends 250-300 base pairs upstream from the 5' terminus of the preproinsulin mRNA. Such a region may allow control sequences special access to regulatory proteins.
D007516:Adenoma, Islet Cell / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000378:metabolism* / Q000648:ultrastructure; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases; D004720:Endonucleases; D005796:Genes; D007328:Insulin; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000378:metabolism; D009928:Organ Specificity; D010190:Pancreatic Neoplasms / Q000378:metabolism; D011384:Proinsulin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats
D002843:Chromatin; D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D004247:DNA; D011384:Proinsulin; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I