Stimulation of encephalomyocarditis virus RNA translation in wheat embryo extracts by rabbit reticulocyte ribosomal wash.
Encephalomyocarditis virus RNA induced a low level of amino acid incorporation in wheat embryo extracts, but the incorporation did not result in the formation of any viral proteins. However, if wheat embryo extracts were supplemented with a ribosomal wash fraction from rabbit reticulocytes, a large stimulation of amino acid incorporation and the formation of encephalomyocarditis virus-specific proteins were obtained. Among the proteins synthesized were capsid precursor proteins which can be processed by an encephalomyocarditis virus-coded protease to produce the viral capsid proteins.
D000818:Animals; D002213:Capsid / Q000096:biosynthesis; D002474:Cell-Free System; D004686:Encephalomyocarditis virus / Q000235:genetics*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis; D012270:Ribosomes / Q000032:analysis; D014908:Triticum; D014764:Viral Proteins / Q000096:biosynthesis
D011498:Protein Precursors; D014764:Viral Proteins