title: A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence.
abstract: A 3200-dalton adrenal enkephalin-containing peptide, designated peptide E, that exhibits high opiate activity in the guinea pig ileum longitudinal muscle preparation was purified, and its structure was determined. It contains an amino-terminal [Met]enkephalin sequence and a [Leu]enkephalin sequence at the carboxyl terminus. Sequence analysis revealed that peptide E arises from a previously characterized 4900-dalton adrenal enkephalin-containing peptide. Peptide E was shown to be 30 times more potent than [Met]enkephalin in the guinea pig ileum assay, which suggests that the adrenal enkephalin-containing peptide may perform a unique biological function in vivo.
mesh_terms: D000313:Adrenal Medulla / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002460:Cell Line; D002837:Chromaffin Granules / Q000032:analysis*; D002838:Chromaffin System / Q000032:analysis*; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000145:classification / Q000378:metabolism*; D005910:Glioma; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D051379:Mice; D008970:Molecular Weight; D009119:Muscle Contraction / Q000187:drug effects; D009447:Neuroblastoma; D011957:Receptors, Opioid / Q000378:metabolism
chemical_list: D004723:Endorphins; D004745:Enkephalins; D011957:Receptors, Opioid; C030962:adrenal opioid peptide E