Foot-and-mouth disease virus production in glass sphere based monolayer systems of 0.1 to 100 L medium volume.
The cell and virus productivity of a 3 mm glass sphere based unit process monolayer system was determined at scales of 0.1, 1, 10 and 100 litres medium capacity. Enhanced yields were obtained up to the 10L size when compared with control Roux bottle cultures. Modifications of the operating conditions of the 100L system should bring this into line with the smaller-scale units.
D000818:Animals; D005537:Aphthovirus / Q000254:growth & development*; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D010948:Viral Plaque Assay; D014776:Virus Cultivation / Q000295:instrumentation / Q000379:methods*