Endogenously formed PGE2 mediates the increase in cAMP accumulation in rabbit splenic fibroblasts following alpha-receptor stimulation.
Prostaglandin E2 synthesis in rabbit splenic fibroblasts was stimulated by noradrenaline but not by isoprenaline. Noradrenaline, isoprenaline and prostaglandin E2 increased intracellular cAMP accumulation. Noradrenaline-, but not isoprenaline-evoked cAMP accumulation was reduced by indomethacin; only combinations of phentolamine or indomethacin with propranolol abolished the noradrenaline effect. We conclude that the noradrenaline-induced increase in cAMP levels of rabbit splenic fibroblasts was due to stimulation of both alpha- and beta-adrenoceptors. The response to alpha-receptor stimulation was possibly mediated by prostaglandin E2.
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011458:Prostaglandins E / Q000378:metabolism* / Q000502:physiology; D011817:Rabbits; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism* / Q000502:physiology; D013154:Spleen / Q000378:metabolism
D011458:Prostaglandins E; D011941:Receptors, Adrenergic; D011942:Receptors, Adrenergic, alpha; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; D009638:Norepinephrine; D007213:Indomethacin; D010646:Phentolamine