Differentiative and proliferative effects of (But)2cAMP, n-butyric acid and prednisolone on the malignant melanoma cell line (TM-1) in vitro and in vivo.
Both (But)2cAMP and n-butyric acid suppressed the proliferation of TM-1 cells and induced the formation and elongation of the cellular processes in vitro. Prednisolone stimulated the proliferation of TM-1 cells and increased the number of polygonal cells in vitro. These results indicated that (But)2cAMP and n-butyric acid induce the differentiation of TM 1 cells, whereas prednisolone brings the cells in an undifferentiated state. The growth of TM-1 cell tumor transplanted into nude mice was suppressed by 5 mg of (But)2cAMP and 1 mg of theophylline. Remarkable histological changes were seen in the tumors treated with these agents as compared with untreated tumors. This suggests that (But)2cAMP suppresses the proliferation of TM-1 cells in vivo.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology* / Q000627:therapeutic use; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy; D011239:Prednisolone / Q000494:pharmacology*; D013806:Theophylline / Q000627:therapeutic use
D002087:Butyrates; D003994:Bucladesine; D011239:Prednisolone; D013806:Theophylline