Improved method for purification of enterotoxin from Clostridium perfringens type A.
The purification procedure of Clostridium perfringens type A enterotoxin has been improved. The cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. The two precipitations were followed by gel filtration on Sephadex G-100. The enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis after this three-step purification procedure, with a recovery of 56% and a 12.3-fold purification. The solubility properties at different pH values, temperatures, and ammonium sulfate concentrations are also given as basis for the purification procedure.
D000645:Ammonium Sulfate; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000032:analysis*; D004768:Enterotoxins / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D012995:Solubility
D004768:Enterotoxins; D000645:Ammonium Sulfate