Role of subunit interactions in the self-assembly of oligomeric proteins.
In oligomeric proteins, the native conformation and its functional properties depend on the interactions which exist between the different chains. The role of these subunit interactions can be studied using either the unfolded state or the native state as a starting point. During the folding process, the properties which appear following a bimolecular reaction are related to the formation of an association area. Similarly, the properties which are lost upon partial dissociation of the native state are related to the association area which is disrupted. Four examples are presented in this article: phosphofructokinase and aspartokinase-homoserine dehydrogenase from E. coli are studied through their folding process, and fatty acid synthetase from B. ammoniagenes and reptilian ovomacroglobulin are studied through their dissociated forms. In all cases, the function of the protein is a sensitive index of the formation of the subunit interactions, and can be more conveniently measured than other size/shape parameters. The extrapolation from the folding of small proteins to the assembly of large and complex structures can be reasonably achieved by admitting that subunit interactions are coupled to the subtle adjustments required by the protein to exert its biological function.
D000818:Animals; D001225:Aspartokinase Homoserine Dehydrogenase / Q000378:metabolism; D001717:Birds; D001951:Brevibacterium / Q000201:enzymology; D004529:Egg White; D004926:Escherichia coli / Q000201:enzymology; D064429:Fatty Acid Synthases / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008259:Macroglobulins / Q000378:metabolism; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011487:Protein Conformation; D011506:Proteins / Q000378:metabolism*; D012104:Reptiles; D013816:Thermodynamics; D000511:alpha-Macroglobulins
D008259:Macroglobulins; D046911:Macromolecular Substances; D011506:Proteins; D000511:alpha-Macroglobulins; C036828:ovomacroglobulin; D064429:Fatty Acid Synthases; D010732:Phosphofructokinase-1; D001225:Aspartokinase Homoserine Dehydrogenase