[Investigation of radiologic lung fine structure by freezing of inflated specimens in liquid nitrogen (author's transl)].
Radiologic pathologic correlation of lung specimens requires a gas content identical to intravital conditions. Methods of air drying and formalin steam fixation are well known but time consuming. A new, quick method is presented: The lungs are inflated with compressed air, frozen in liquid nitrogen, and cut into slices. Radiographs taken in soft tissue technique will demonstrate structural details down to 200--300 mu. The excellent state of preservation enables unusual quality of microscopic slides, as well.
D000328:Adult; D018288:Carcinoma, Small Cell / Q000473:pathology; D005615:Freezing; D006652:Histological Techniques; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008168:Lung / Q000000981:diagnostic imaging / Q000473:pathology*; D008171:Lung Diseases / Q000473:pathology*; D055370:Lung Injury; D008175:Lung Neoplasms / Q000473:pathology; D009584:Nitrogen; D011859:Radiography; D013048:Specimen Handling; D014021:Tissue Preservation
D009584:Nitrogen
Die röntgenologische Feinstruktur der Lunge. II. Gefrierhärtung isolieter Lungen in flüssigem Stickstoff.