The structure of R1drd19: a revised physical map of the plasmid.
We have analyzed derivatives of the plasmid R1drd19 carrying the transposon Tn10 by electron microscopy following denaturation and renaturation of the molecules, and by digestion with various restriction enzymes, gel electrophoresis and Southern blotting. We show: 1) that the published restriction map of R1drd19 is inconsistent with our results. We present a modified map which is consistent with our data. 2) that R1drd19 carries a single resident copy of the element IS10 which is normally associated with Tn10 as an inverted repeat, and 3) that R1drd19 carries three copies of the insertion element IS1 in the resistance determinant region.
D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D011815:R Factors; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes