title: Regulation of neuron-specific enolase in NG108-15 hybrid cells and C6BU-1 glioma cells.
abstract: Distribution of three isoenzymes of brain enolase (2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11) (alpha alpha, alpha gamma and gamma gamma forms) in clonal cell lines of neuroblastoma (NS20Y and N18TG-2), glioma (C6BU-1), and hybrid cells NG108-15, NCB20, Nbr10A, Nbr20A, N4G-B-a and N4G-C-a) was examined with a sensitive enzyme immunoassay system, that uses a rabbit antibody to rat brain enolase alpha alpha or gamma gamma. All cell lines tested were found to possess the enolase which contains gamma subunit (a neuron-specific protein), although the alpha alpha enolase (non-neuronal enolase) was the dominant from in these cells. A clonal rat glioma (C6BU-1) cell contained about 40, 1 and 0.07 microgram/mg protein of alpha alpha, alpha gamma and gamma gamma enolases, respectively, at the confluent stage. Inclusion of 1 mM dibutyryl cyclic AMP or 10 micrometers prostaglandin E1 plus 1 mM theophylline in the culture medium of a hybrid cell (NG108-15, mouse neuroblastoma x rat glioma) resulted in a more than 2-fold increase in the concentrations of alpha gamma and gamma gamma in the cell within a few days, with little change in the alpha alpha enolase concentration. A similar increase in the concentration of gamma subunit by the nucleotide (but not by prostaglandin E1 plus theophylline) was also observed in the glioma cell (C6BU-1) line. The results suggest that the gamma subunit or the neuron-specific protein can be regulated in NG108-15 and C6BU-1 cells in a cyclic AMP-dependent fashion.
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002999:Clone Cells; D006224:Cricetinae; D004790:Enzyme Induction; D005910:Glioma / Q000201:enzymology; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D046911:Macromolecular Substances; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology; D009474:Neurons / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D013806:Theophylline / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D011458:Prostaglandins E; D003994:Bucladesine; D013806:Theophylline; D010751:Phosphopyruvate Hydratase