On the activation of fructose-1,6-bisphosphatase of spinach chloroplasts and the regulation of the Calvin cycle.
Activation by light of spinach fructose-1,6-bisphosphatase is mimicked by dithiothreitol. This process of activation by dithiothreitol implies the specific reduction of two disulfide bridges and a conformation change of the enzyme that makes eight sulfhydryl groups available. The activated enzyme has an apparent allosteric kinetic behavior with respect to both fructose 1,6-bisphosphate and magnesium.
D001665:Binding Sites; D002245:Carbon Dioxide / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010777:Photochemistry; D011487:Protein Conformation / Q000187:drug effects; D014675:Vegetables
D002245:Carbon Dioxide; D006597:Fructose-Bisphosphatase; D004229:Dithiothreitol