Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein.
The polypeptide product of the lon (capR) gene was identified and partially purified from bacterial strains homozygous for the capR(+) or capR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR(+) and capR9 proteins bind DNA.
D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D008970:Molecular Weight; D009154:Mutation
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D004247:DNA