Alternative synthesis of corticotropin-like intermediate lobe peptide (human CLIP). A peptide corresponding to the sequence of human ACTH-(18--39).
A novel synthesis of human CLIP, a peptide corresponding to the sequence of human ACTH-(18-39) is described. The dodecapeptide chain was assembled by a combination of fragment condensation and stepwise synthesis while using N alpha-benzyloxycarbonyl and side chain tert.-butyl-derived protective groups combination. The final deprotection was performed by acidolysis in trifluoroacetic acid. The end product was purified by ion exchange chromatography on carboxymethyl cellulose.
D000324:Adrenocorticotropic Hormone / Q000138:chemical synthesis*; D000595:Amino Acid Sequence; D002855:Chromatography, Thin Layer; D053497:Corticotropin-Like Intermediate Lobe Peptide; D010446:Peptide Fragments / Q000138:chemical synthesis*
D010446:Peptide Fragments; D053497:Corticotropin-Like Intermediate Lobe Peptide; D000324:Adrenocorticotropic Hormone