title: Epstein-Barr virus in nontumorigenic and tumorigenic nasopharyngeal carcinoma (NPC) somatic cell hybrids.
abstract: Somatic cell hybrids between mouse fibroblasts and human cells derived from nasopharyngeal carcinoma (NPC) biopsies or NPC tumors propagated in nude mice were examined for the expression of the Epstein-Barr nuclear antigen (EBNA), retention of Epstein-Barr viral (EBV) DNA, and tumorigenicity in nude mice. In all hybrids the expression of EBNA correlated with the detection of EBV-DNA. After more than 2 years in culture, the hybrids examined retained similar amounts of EBV-DNA when compared to previously published data. Retention of EBV-DNA did not correlate with the presence of any particular human chromosome. Use of either rodent cell lines, clone 1D or IT-22, did not affect the retention nor loss of EBV-DNA. For tumorigenicity studies, NPC cells were fused with IT-22 cells and injected into nude mice. Tumor formation did not depend on the presence or absence of EBNA and detectable EBV-DNA sequences; tumorigenicity in these studies could not be correlated with the presence of any particular human chromosome or the origin of the NPC biopsy.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002277:Carcinoma / Q000382:microbiology*; D002460:Cell Line; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000032:analysis*; D006822:Hybrid Cells / Q000382:microbiology*; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology*; D009369:Neoplasms / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral