A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.
Lambda derivatives are described that can be used for cloning DNA fragments of about 20 kilobase pairs (kb) generated by restriction enzymes EcoRi, HindIII, BamHI, MboI and BglII. Recombinants can be selected by their Spi- phenotype and their propagation is facilitated by the presence of a chi site.
D010582:Bacteriophage lambda / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011995:Recombination, Genetic; D012641:Selection, Genetic; D014762:Viral Interference
D004262:DNA Restriction Enzymes