title: The 5'-nucleotidase complex in sera from patients with gall stones--electrophoretic patterns and substrate specificity.
abstract: Various purine and pyrimidine mononucleotides seem to be dephosphorylated by a 5'-nucleotidase complex composed of four fractions in sera from both patients with gall stones and from normal subjects. Agarose gel electrophoresis, with the addition of Triton X-100, demonstrated that the faster (F-) fraction of the enzyme complex and the slower (S-) fraction both consisted of two sub-fractions (termed alpha and beta). The enzyme complex showed a uniform substrate specificity pattern, which was independent of the absolute enzyme activity.
mesh_terms: D015720:5'-Nucleotidase; D000328:Adult; D000368:Aged; D002769:Cholelithiasis / Q000201:enzymology*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009708:Nucleotidases / Q000097:blood*; D017830:Octoxynol; D011092:Polyethylene Glycols; D013379:Substrate Specificity
chemical_list: D011092:Polyethylene Glycols; D017830:Octoxynol; D009708:Nucleotidases; D015720:5'-Nucleotidase