Sucrose-gap recordings of nerve-evoked potentials in mammalian parasympathetic ganglia.
The sucrose-gap recording technique was used to study mammalian parasympathetic ganglionic transmission. Both a fast nicotinic depolarization potential and a slow muscarinic hyperpolarizing potential were recorded in vesical pelvic ganglia (VPG). A long afterhyperpolarization caused by an electrical response of through-fibers was also recorded. However, a slow excitatory postsynaptic potential (S-EPSP) was not readily observed and may be masked by the long afterhyperpolarization. In addition, the slow inhibitory postsynaptic potential (S-IPSP) of the VPG was due to a direct effect of acetylcholine (ACh). Thus, sucrose-gap recordings of VPG potentials are similar to those obtained in sympathetic ganglia, but the mechanism for transmission of the S-IPSP may be different in the respective ganglia.
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D002415:Cats; D005071:Evoked Potentials; D005726:Ganglia, Parasympathetic / Q000502:physiology*; D009130:Muscle, Smooth / Q000502:physiology; D009412:Nerve Fibers / Q000502:physiology; D011976:Receptors, Muscarinic / Q000502:physiology; D011978:Receptors, Nicotinic / Q000502:physiology; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission; D001743:Urinary Bladder / Q000294:innervation
D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D000109:Acetylcholine