[Comparison of physico-chemical properties of two forms of dopamine-beta-hydroxylase from chromaffin granules].
The purification procedures of both soluble and membrane-bound forms of dopamine-beta-hydroxylase have been developed. The membrane-bound form was solubilized by a detergent. Both preparations have been obtained in electrophoretically homogeneous form. The yield of soluble and membrane-bound enzyme forms by a described procedure was 22 mg and 15 mg, correspondingly, from 100 g of chromaffin granules paste. A comparative analysis of the main physico-chemical properties of the two enzyme forms has shown their identity. The effects of pH, ionic strength, oxidants and reducers on the EPR spectra of the two forms of dopamine-beta-hydroxylase have been investigated. A comparison of the EPR spectra of the two forms of the enzyme suggests that the copper environment of soluble and membrane-bound dopamine-beta-hydroxylases is practically identical.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002837:Chromaffin Granules / Q000201:enzymology*; D002838:Chromaffin System / Q000201:enzymology*; D003300:Copper / Q000032:analysis; D004299:Dopamine beta-Hydroxylase / Q000302:isolation & purification / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation
D007527:Isoenzymes; D003300:Copper; D004299:Dopamine beta-Hydroxylase
Sravnitel'noe izuchenie fiziko-khimicheskikh svoĩstv dvukh form dofamin-beta-gidroksilazy iz khromaffinykh granul.