A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase.
A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase is described. The assay involves separation of the substrate and product by ion-exchange chromatography followed by treatment of pyridoxal with potassium cyanide under slightly alkaline conditions to form 4-pyridoxolactone, a highly fluorescent compound. Certain kinetic properties of the enzyme activities in human neutrophils are described.
D002852:Chromatography, Ion Exchange; D010084:Oxidation-Reduction; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
C027138:pyridoxine phosphate phosphatase; D010744:Phosphoric Monoester Hydrolases