Transcriptional initiation and 5' termini of yeast mitochondrial RNA.
We have used vaccinia virus guanylyltransferase to label polyphosphate-terminated yeast mitochondrial RNAs in vitro with [alpha-32P]GTP. Hybridization of RNA labeled in vitro indicates the presence of multiple transcriptional initiation sites in both grande and petite mitochondrial genomes. Agarose/urea gel electrophoresis of capped RNA suggests the existence of a precursor to the small (14 S) rRNA. In contrast, direct examination of the large (21 S) rRNA by partial ribonuclease T1 digestion reveals a complete lack of processing of the 5' end of the primary transcript of this RNA.
D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D006157:Guanosine Monophosphate / Q000032:analysis; D006160:Guanosine Triphosphate / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009713:Nucleotidyltransferases / Q000378:metabolism; D012315:RNA Caps / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000201:enzymology
D004272:DNA, Mitochondrial; D012315:RNA Caps; D006157:Guanosine Monophosphate; D006160:Guanosine Triphosphate; D009713:Nucleotidyltransferases; C020842:guanylyltransferase; D004262:DNA Restriction Enzymes