title: Functional organization of plasmid pKM101.
abstract: Tn5 insertion mutants and in vitro-generated deletion mutants of the mutagenesis-enhancing plasmid pKM101 have been used to identify several genetic regions on the pKM101 map. In clockwise order on the pKM101 map are: (i) the bla gene, coding for a beta-lactamase; (ii) the Slo region, responsible for retarding cell growth on minimal medium; (iii) the tra genes, enabling pKM101 to transfer conjugally; (iv) sensitivity to IKe phage (this function[s] maps within the tra region); (v) the muc gene(s), responsible for enhancing ultraviolet light and chemically induced mutagenesis in the cell; and (vi) the Rep region, essential for plasmid replication. The muc gene(s) and the Rep region are contained in a deoxyribonucleic acid region bounded by inverted repeated sequences.
mesh_terms: D003090:Coliphages / Q000254:growth & development; D003227:Conjugation, Genetic; D004260:DNA Repair; D004261:DNA Replication; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D001618:beta-Lactamases