Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus.
Fastidious human rotaviruses that did not undergo productive infection in tissue culture were rescued by genetic reassortment during mixed infection with a temperature-sensitive (ts) mutant of a cultivatable bovine rotavirus. In this manner, the genes of the fastidious rotavirus that restricted growth in vitro were replaced by the corresponding genes from a tissue culture-adapted rotavirus. We recovered genetically reassorted viruses that grew to high titer and were neutralized specifically by hyperimmune guinea pig type 1 or type 2 human rotavirus antiserum. Preliminary RNA analysis of these clones disclosed that they were indeed viruses with reassorted genes.
D000818:Animals; D002522:Chlorocebus aethiops; D007668:Kidney; D009154:Mutation; D009500:Neutralization Tests; D011995:Recombination, Genetic; D012087:Reoviridae / Q000254:growth & development*; D012401:Rotavirus / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D013696:Temperature; D014776:Virus Cultivation