[Ca2+ inhibition of adenyl cyclase of rabbit jejunal mucosa].
Ca2+ ions at a concentration of 10-7--10-3 M inhibit adenylate cyclase of the rabbit jejunum mucosa (K0.5= =10-4 M). This effect is not modified after the membrane extraction by 10-3 M EDTA solution with a high or low ionic strength or after removal from the membrane preparation of Ca2+-dependent thermostable regulatory protein. The inhibition by Ca2+ions was discovered also in the presence of adenylate cyclase irreversible activators (guanylylimidodiphosphate and NaF), which points to the spontaneous interaction of Ca2+ ions with the catalytic subunit of the adenylate cyclase complex.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000032:analysis / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology; D011817:Rabbits
D000067956:Adenylyl Cyclase Inhibitors; D011506:Proteins; D006165:Guanylyl Imidodiphosphate; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002118:Calcium
Ingibirovanie ionami Ca2+ adenilattsiklazy slizistoĭ obolochki toshcheĭ kishki krolika.