title: Characterization of JC papovavirus adapted to growth in human embryonic kidney cells.
abstract: Human papovavirus JC virus was adapted to growth in human embryonic kidney (HEK) cells. After eight passages, the HEK-adapted JC virus produced high virus yields and was capable of forming plaques in HEK monolayer cultures. Eleven plaque-purified stocks were prepared and characterized. Biologically, the plaque-purified virus induced tumor and viral antigens in HEK cells earlier and in a higher percentage of cells than uncloned virus. Cytopathic changes were also evident sooner and were more extensive. The DNA from uncloned as well as plaque-purified isolates was analyzed by restriction endonuclease cleavage followed by gel electrophoresis. The DNA from uncloned HEK-adapted virus was heterogeneous. Plaque-purified virus isolates yielded DNA which, although much less heterogeneous than the uncloned stock, still consisted of two or more species of viral DNA.
mesh_terms: D002478:Cells, Cultured; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004622:Embryo, Mammalian; D006801:Humans; D007668:Kidney / Q000166:cytology*; D011120:Polyomavirus / Q000254:growth & development*; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes