Characterization of the 5'-terminal cap structures of early simian virus 40 mRNA.
Early simian virus 40-specific mRNA was isolated from lytically infected and stably transformed cells and analyzed with respect to the 5'-terminal cap content. An analogous diversity of capped structures was found in both RNA preparations, indicating that 5'-terminal heterogeneity is not limited to late simian virus 40 RNA transcription but concerns a more general feature. Correlation of our data with previously published results of characterization of early mRNA's by reverse transcription allowed a tentative localization of the major 5' termini on the viral genome.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D012315:RNA Caps / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D013539:Simian virus 40 / Q000032:analysis* / Q000502:physiology
D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral