Characterization by immunoperoxidase of the lymphocytes with bundle-shaped tubular (BST) inclusions in human normal peripheral blood.
In six normal subjects, no surface immunoglobulins were detected on blood lymphocytes containing bundle-shaped tubular (BST) inclusions, after incubation with anti-human IgM peroxidase-labeled Fab'2 fragments. These Fab'2 fragments from pepsin-digested sheep anti-human immunoglobulins revealed human mu kappa, and lambda chains. These results suggest that the BST inclusions do not belong to B lymphocytes but belong to T cells and/or a third lymphocyte population, including K cells and precursors of T and B cells.
D000818:Animals; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D002479:Inclusion Bodies / Q000648:ultrastructure*; D007945:Leukemia, Lymphoid / Q000276:immunology; D008214:Lymphocytes / Q000648:ultrastructure*; D011947:Receptors, Antigen, B-Cell; D012751:Sezary Syndrome / Q000276:immunology; D012756:Sheep
D007140:Immunoglobulin Fab Fragments; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D011947:Receptors, Antigen, B-Cell