title: The effect of bilirubin on the function of hamster small intestine.
abstract: Jaundice phototherapy is associated with a significant incidence of watery diarrhea. We have postulated that acute intestinal secretion, rather than malabsorption of dietary carbohydrate, is an effect of a photoproduct of bilirubin upon the intestinal mucosa. Because of major effect of phototherapy is the hepatic excretion of nonconjugated bilirubin, we investigated the effect of bilirubin on small intestinal function in the hamster in vivo. The entire small intestine was luminally perfused in vivo with solutions containing bilirubin (0.125 to 0.75 mmole/liter) and net water and sodium fluxes were measured. Control animals absorbed both water (J H2O(net) = 58.9 microliter/min/g) and sodium (J Na(net) = 4.55 microEq/min/g), but animals perfused with bilirubin (greater than or equal to 0.25 mmole/liter) exhibited secretion of water (J H2O(net) = -39.0--85.9) and sodium (J Na(net)=-9.91--18.24). The rate of water secretion was positively related to the concentration of bilirubin in the infusate (r=0.749; p less than 0.001). The concentration of bilirubin in ultrafiltrates of perfusate was likewise positively related to its concentration in the infusate (r = 0.844; p less than 0.001), indicating the potential importance of soluble forms of bilirubin in inducing secretion. Possible epithelial injury was studied by measuring the concentration of DNA in the perfusate and the activity of disaccharidases in postperfusion mucosa, and the possible role of cyclic adenosine monophosphate as a mediator of the secretory process was investigated by determining its concentration in postperfusion mucosa. Perfusion with 0.5 mM bilirubin, which produced significant secretion, did not cause loss of DNA (0.284 versus 0.244 mg/liter) or mucosal lactase activity (56 versus 53 units/g) or enhancement of cyclic adenosine monophosphate concentration (14.9 versus 14.12 pmoles/mg protein).
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000494:pharmacology*; D001834:Body Water / Q000378:metabolism; D006224:Cricetinae; D003576:Electron Transport Complex IV / Q000032:analysis*; D004817:Epidermis / Q000201:enzymology; D007413:Intestinal Mucosa / Q000378:metabolism; D007419:Intestinal Secretions / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D010477:Perfusion; D010789:Phototherapy / Q000009:adverse effects; D012964:Sodium / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000032:analysis*
chemical_list: D012964:Sodium; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D001663:Bilirubin