Early nuclear antigen as DNA-binding protein in cytomegalovirus-infected cells.
Early nuclear complement-fixing antigen in cytomegalovirus (CMV) (strain Ad 169)-infected human embryonic fibroblasts is described. The nuclear antigen was solubilized from CMV-infected cells by high salt treatment. DNA-binding properties were studied by DNA-cellulose chromatography. The purified antigen, eluted from double-stranded DNA-cellulose columns, was added to acid-fixed nuclear preparations from human embryonic fibroblasts and then exposed to human sera containing antibodies to CMV. Positive staining was obtained by anti-complement immunofluorescence. These data identify the CMV-determined nuclear antigen as a DNA-binding protein. In this respect, it is similar to the Epstein-Barr virus nuclear antigen.
D000956:Antigens, Viral / Q000302:isolation & purification; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology*; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins; D006801:Humans; D014764:Viral Proteins / Q000378:metabolism*
D000956:Antigens, Viral; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D004247:DNA