Membrane-bound and water-soluble cytochrome c1 from Neurospora mitochondria.
Cytochrome c1 is a subunit of ubiquinol--cytochrome c reductase (EC 1.10.2.2). In Neurospora crassa wild type 74A grown in the presence of chloramphenicol, the subunit is inserted only into the bilayer of the mitochondrial inner membranes without associating with other proteins. From these modified membranes a monodisperse (cytochrome c1)-Triton complex was isolated by subjecting the Triton-solubilized membranes to affinity chromatography on immobilized cytochrome c. A water-soluble pentamer of cytochrome c1 was prepared from the (cytochrome c1)-Triton complex by removing the detergent. By limited proteolytic digestion of the cytochrome c1-Triton complex with chymotrypsin, a water-soluble monomeric cytochrome c1 was prepared which has a molecular weight of only 24 000 as compared to 31 000 of the membrane-bound cytochrome c1. The 24 000-Mr cytochrome c1 and the 31 000-Mr cytochrome c1 have same light absorption spectra and cytochrome-c-binding properties. These results are used to propose the following model. Cytochrome c1 consists of a large hydrophilic part and a small hydrophobic part. The hydrophilic part extends from the mitochondrial inner membrane into the intermembrane space. This part carries the heme and interacts with cytochrome c. The hydrophobic part anchors the cytochrome c1 to the bilayer.
D000596:Amino Acids / Q000032:analysis; D003574:Cytochrome c Group / Q000031:analogs & derivatives*; D003575:Cytochromes c1 / Q000302:isolation & purification* / Q000378:metabolism; D003902:Detergents; D007425:Intracellular Membranes / Q000378:metabolism; D008051:Lipid Bilayers; D008823:Micelles; D008928:Mitochondria / Q000378:metabolism*; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D017830:Octoxynol; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols; D013053:Spectrophotometry
D000596:Amino Acids; D003574:Cytochrome c Group; D003902:Detergents; D008051:Lipid Bilayers; D008823:Micelles; D011092:Polyethylene Glycols; D017830:Octoxynol; D003575:Cytochromes c1