Karyological and isoenzyme characterization of established human sarcoma cell lines.
Six established human sarcoma cell lines (giant cell tumor of bone B-5GT, fibrosarcoma, B-6FS, cystosarcoma phylloides B-19CS, synovial sarcoma U-4SS and two osteogenic sarcomas U-20S and U-393OS) have been studied and compared to the normal B-41FB fibroblastic cells and the HeLa cells. For cytogenetic and isoenzyme characterization both conventional and G banding techniques as well as the mobility of lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PD) were employed. All six sarcoma cell lines had karyotype and LDH patterns of human cells. A study of the chromosome counts distribution revealed a large variability from one sarcoma cell line to the other. There was no evidence of any cross-contamination between the lines or by HeLa cells. This conclusion is based on the detection of G-6-PD with B phenotype, the lack of HeLa marker chromosomes in all sarcoma lines and the presence of Y chromosome in U-4SS and U-393OS derived from male donors. In addition, each sarcoma cell line revealed a group of distinctive marker chromosomes which can serve to identify them and help to control cell line specificity during in vitro culturing.
D002460:Cell Line; D002875:Chromosomes; D005260:Female; D005952:Glucose-6-Phosphatase / Q000235:genetics; D006367:HeLa Cells / Q000473:pathology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D010641:Phenotype; D012509:Sarcoma / Q000201:enzymology / Q000473:pathology*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005952:Glucose-6-Phosphatase