The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.
We have determined the nucleotide sequence of a 5.5-kilobase segment of cloned mouse DNA which includes regions encoding two parts of the mouse kappa immunoglobulin gene: the J regions (amino acids 96-108 of the kappa chain) and the C region (residues 109-214). This sequence allows us to rule out interruptions in the germline constant region coding segment as well as the presence of additional functional J genes in the sequenced DNA segment, although two weak homologies to J regions have been found. The complete sequence also allows us to identify a single occurrence of the heptanucleotide palindrome thought to play a role in V/J joining. This palindrome, midway between J and C regions, is the site of aberrant joining in the plasmacytoma MPC11 and may be a target for such aberrant recombination of other kappa genes. In addition, computer analysis of the J sequences suggests that those closest to the C region arose by the most recent duplication event.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000276:immunology*; D005796:Genes; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight
D004274:DNA, Recombinant; D007127:Immunoglobulin Constant Regions; D007144:Immunoglobulin J-Chains; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007136:Immunoglobulins; D004262:DNA Restriction Enzymes