Thiamphenicol as an inhibitor of early red cell differentiation.
The effects of an in vivo treatment of mice with thiamphenicol on stem cells are shown. Thiamphenicol causes a drastic depletion of erythroid precursors in the marrow. Mitochondrial protein synthesis is inhibited resulting in a severely reduced cell proliferation. The number of pluripotent stem cells in the marrow did not decrease. In the spleens of thiamphenicol-treated mice a strong reduction of stem cells was found. The sedimentation behaviour of stem cells from anemic and thiamphenicol-treated mice was similar. The pluripotent stem cells from thiamphenicol-treated mice were in a low cycling state, despite a very high erythropoietin level. Under these circumstances a partial commitment of the pluripotent stem cells into the erythroid direction was observed.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004261:DNA Replication / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D013154:Spleen / Q000502:physiology; D013839:Thiamphenicol / Q000494:pharmacology*
D003576:Electron Transport Complex IV; D013839:Thiamphenicol