Inhibition of rhinovirus replication in in organ culture by a potential antiviral drug.
The compound 2-amino-1-(isopropyl sulfonyl)-6-benzimidazole phenyl ketone oxime (LY122771-72) at a concentration of 0.2 microgram/ml completely inhibited rhinovirus replication in human embryonic nasal organ cultures, although in the absence of virus the compound did not inhibit ciliary activity when used at a concentration of 25 micrograms/ml. When added 26 hr after infection, the compound stopped rhinovirus production in organ cultures that had already started to release virus. Five rhinovirus types available for infection of volunteers and six recently obtained clinical isolates were shown to be more sensitive to LY122771-72 in tissue culture than the rhinovirus type 31 used in the organ culture experiments. These results suggest that this potential antiviral drgu should be evaluated in humans.
D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium; D006801:Humans; D008826:Microbial Sensitivity Tests; D009666:Nose / Q000196:embryology; D009924:Organ Culture Techniques; D010091:Oximes; D012229:Rhinovirus / Q000187:drug effects / Q000254:growth & development*; D013449:Sulfonamides; D014132:Trachea / Q000166:cytology / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D001562:Benzimidazoles; D010091:Oximes; D013449:Sulfonamides; C021595:viroxime