Characteristics of antisera against periodate-resistant membrane antigens from Neisseria gonorrhoeae.
Crude outer membrane (OM) was prepared by extraction of bacteria of the Neisseria gonorrhoeae strains 8551. V, and VII, with an EDTA-containing buffer. The preparations contained the lipopolysaccharide (LPS) and at least 10 proteins as shown by SDS-polyacrylamide gel electrophoresis. Immunization of rabbits with untreated OM resulted in production of antibodies against several antigens, including LPS. Antisera raised against periodate-treated OM did not contain antibodies against LPS. These latter antisera agglutinated heat-treated (100 degrees C, 60 min) gonoccal cells by means of antibodies to one or more common agglutinogens and against a strain-specific agglutinogen that was susceptible to digestion with proteolytic enzymes. Both side agglutination and a plate agglutination test could be used to detect antibodies against these agglutinogens.
D000371:Agglutination; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D007106:Immune Sera; D008070:Lipopolysaccharides / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D010504:Periodic Acid / Q000494:pharmacology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010504:Periodic Acid