Inhibition of mengovirus RNA-dependent RNA polymerase by an isatinisothiosemicarbazone and a piperidine-thiocarbonyl-hydrazone derivative in a cell-free system.
The inhibitory action of two antiviral compounds used at the maximum tolerated dosis in a cellular system led to a complete suppression of the infectious mengovirus yield and to a 100% plaque reduction. The products of the RNA polymerase reaction catalyzed by the microsomal-mitochondrial fraction of mengovirus-infected FL cells were analyzed by linear sucrose gradient centrifugation and polyacrylamid gel electrophoresis. The results showed that the inhibitors cause a general reduction of the synthesis of single-stranded viral RNA. The influence on double-stranded viral RNA was more obvious in the case of the isatinisothiosemicarbazone derivative.
D000998:Antiviral Agents / Q000494:pharmacology; D006835:Hydrazones / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000031:analogs & derivatives / Q000494:pharmacology*; D008574:Mengovirus / Q000187:drug effects* / Q000201:enzymology; D010880:Piperidines / Q000494:pharmacology*; D012316:RNA Nucleotidyltransferases / Q000032:analysis*; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis*; D013859:Thiocarbamates / Q000494:pharmacology*; D013882:Thiosemicarbazones / Q000494:pharmacology*
D000998:Antiviral Agents; D006835:Hydrazones; D007211:Indoles; D010880:Piperidines; D013859:Thiocarbamates; D013882:Thiosemicarbazones; D007510:Isatin; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase