title: A gene chimaera of SV40 and mouse beta-globin is transcribed and properly spliced.
abstract: A gene chimaera of the first exon from simian virus 40 (SV40) sequences coding for T antigen placed upstream from the third exon from mouse beta-globin, and separated by the resultant new chimaeric intron, was cloned into a bacterial plasmid. When transfected into monkey cells, the gene chimaera was transcribed, polyadenylated and spliced using the donor splice site from SV40 and the acceptor splice site from mouse beta-globin. This result suggests that a donor site from one gene can be spliced to an acceptor site from another gene.
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D000956:Antigens, Viral / Q000235:genetics*; D000957:Antigens, Viral, Tumor; D001483:Base Sequence; D002678:Chimera; D004274:DNA, Recombinant; D005914:Globins / Q000235:genetics*; D009694:Nucleic Acid Precursors / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D011061:Poly A; D005914:Globins