title: Cloning of hepatitis A virus genome.
abstract: Hepatitis A virus (HAV) was highly purified from faeces. The genomic RNA was transcribed to cDNA and this DNA was then cloned into plasmid pBR 322 at the Pst I site, and clones were selected in presence of tetracycline. Most clones contained inserts which hybridized to HAV-specific RNA isolated from HAV-infected cell cultures derived from a human hepatocellular carcinoma. Two clones expressed low amounts of viral antigens.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D006507:Hepatovirus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D004247:DNA