title: Restriction mapping of the DNA of the Streptomyces temperate phage phi C31 and its derivatives.
abstract: DNA of phi C31 propagated on Streptomyces lividans 66 contained no sites for the restriction enzymes BamHI, SalPI (=PstI) and XhoI; one for XbaI; three for HpaI; five for ClaI and KpnI; six for EcoRI; about 13 for HindIII; about 14 for BclI; and more than 15 for FspAI, HgiAI, SacI, SalGI and SmaI. A complete map of 20 sites (XbaI, HapI, ClaI, KpnI and EcoRI) was obtained using partial digestion and double digestion of DNA of the wild-type and deletion and insertion mutants. The total molecular size was estimated to be 41.2 kb.
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D013302:Streptomyces / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes