title: Considerations on the value of freeze-etching technique in studying the ultrastructure of some anaerobic bacteria.
abstract: Samples from periodic cultures of Clostridium perfringens, Clostridium oedematiens, Clostridium tetani and Bifidobacterium bifidum grown in liquid nutrient media were studied after freeze-fracturing and etching without pretreatment by means of chemical agents including cryo-protectants. Subunits in the cell all of Cl. tetani and differences in the distribution of globular particles in the plasma membrane of the investigated bacteria are revealed.
mesh_terms: D000191:Actinomycetaceae / Q000648:ultrastructure*; D002462:Cell Membrane / Q000648:ultrastructure; D003013:Clostridium / Q000648:ultrastructure*; D003016:Clostridium perfringens / Q000648:ultrastructure; D003017:Clostridium tetani / Q000648:ultrastructure; D003451:Cryoprotective Agents; D005613:Freeze Etching / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods
chemical_list: D003451:Cryoprotective Agents