Incorporation of 14C-adenine to cyclic 3',5'-AMP of cardiac muscle in isoprenaline-induced experimental cardiomyopathy.
Experimental cardiomyopathy was produced by isoprenaline (2 x 80 mg/kg sc daily for 2 days). The degree of myocardial damage was evaluated histopathologically. In the damaged cardiac muscle the incorporation of 14C-adenine was impaired, the adenylate cyclase activity was diminished and the total contents of cAMP and 14C-cAMP formed from intracellular 14C-adenine metabolism were reduced. No significant changes in the activity of phosphodiesterase were found. This indicates that cAMP synthesis is impaired as a result of decreased utilization of the ATP pool formed from exogenous adenine in the cardiac muscle damaged by isoprenaline.
D000225:Adenine / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D009202:Cardiomyopathies / Q000139:chemically induced / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis*; D007545:Isoproterenol; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051381:Rats
D002250:Carbon Radioisotopes; D000242:Cyclic AMP; D000225:Adenine; D007545:Isoproterenol