Dynamic light scattering studies of ribonuclease.
Dynamic light scattering has been used to measure the translational diffusion coefficients of bovine pancreatic ribonuclease A as functions of temperature and concentration in the presence of 1 M Guanidine-HCl. Data was collected throughout a temperature range including the folding-unfolding transitions. Evidence of a pretransition "swelling" of the protein was observed. Entropy and enthalpy changes upon unfolding were obtained using a two-state model.
D000818:Animals; D002417:Cattle; D004720:Endonucleases; D008027:Light; D008433:Mathematics; D010179:Pancreas / Q000201:enzymology; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases; D012542:Scattering, Radiation; D013816:Thermodynamics
D004720:Endonucleases; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic