title: DNA cloned from the ayw subtype of hepatitis B virus.
abstract: DNA from the ayw subtype of hepatitis B virus (HBV) was ligated into the EcorI site of DNA from plasmid pBR322 and propagated in E coli chi 776. A plasmid with a 3200 base pair insert (pHBV-1) was isolated and the cloned HBV DNA was mapped with restriction endonucleases. Differences were found in restriction endonuclease cleavage sites for DNAs from HBV of subtype ayw and adr.
mesh_terms: D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000032:analysis* / Q000235:genetics; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D004262:DNA Restriction Enzymes