[Dansylated amino acid separation by polyacrylamide gel electrophoresis].
The paper describes a method for separation of dansylated amino acids by polyacrylamide gel electrophoresis. The methods allows a simultaneous analysis of 20-30 samples. The sensitivity of the method is 1 x 10(-9)-1 x 10(-10) M amino acid. The method permits separation of all amino acids formed during acid hydrolysis of proteins except for two pairs: Ile, Phe and Val, Asp.
D000596:Amino Acids / Q000302:isolation & purification*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens / Q000378:metabolism; D002918:Chymotrypsin; D003574:Cytochrome c Group; D003619:Dansyl Compounds / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006657:Histones; D008163:Luminescent Measurements; D014357:Trypsin
D000596:Amino Acids; D003574:Cytochrome c Group; D003619:Dansyl Compounds; D006657:Histones; D002918:Chymotrypsin; D014357:Trypsin
Razdelenie dansilirovannykh aminokislot metodom élektroforeza v poliakrilamidnom gele.