In vitro synthesis of the full-length complement of the negative-strand genome RNA of vesicular stomatitis virus.
Under the normal conditions of in vitro RNA synthesis, the virion-associated RNA polymerase of vesicular stomatitis virus synthesizes five monocristronic mRNAs and a 48-nucleotide-long leader RNA that represents the exact 3'-terminal region of the genome RNA [Colonno, R. J. & Banerjee, A. K. (1978) Cell, 15, 93-101]. When the transcribing core was preincubated with ATP and CTP, reisolated, and then incubated in the presence of the beta, gamma imido analogue of ATP (AdoPP[NH]P) and the three normal ribonucleoside triphosphates, the full-length complementary strand of the genome RNA was synthesized in vitro. The results suggest that specific phosphorylated states of regulatory proteins may control transcription in vitro to generate the full-length plus strands.
D000255:Adenosine Triphosphate / Q000378:metabolism; D003570:Cytidine Triphosphate / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000494:pharmacology; D005814:Genes, Viral; D008970:Molecular Weight; D010750:Phosphoproteins / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*; D014779:Virus Replication
D003838:Deoxyadenine Nucleotides; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D003570:Cytidine Triphosphate; D000255:Adenosine Triphosphate