SV40-transformed simian cells support the replication of early SV40 mutants.
CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.
D002460:Cell Line; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D003673:Defective Viruses / Q000235:genetics; D005814:Genes, Viral; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics*; D013696:Temperature; D014779:Virus Replication