[Cytochemical demonstration of cyclic nucleotide phosphodiesterases in the taste buds of Testudo horsefieldi turtles upon exposure to flavored substances].
Phosphodiesterase of cyclic nucleotides was found cytochemically on the plasmat membrane of the apical part and microvillar projections of the receptor cells and, to a lower extent on the membranes of microvilli of the supporting cells as well as on the mucose granules of the taste buds of the tortoise Testudo horsfieldi. Administration of 1/250 M quinine chloride to the surface of the tongue results in the disappearance of the products of the enzymic reaction from the surface of the mentioned membranes. After application of 0.25 M surcrose, the reaction products are found within the apical part of the receptor cells on the membranes of the plain endoplasmic reticulum. Possible role of cyclic nucleotides in taste reception is discussed.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006651:Histocytochemistry; D008854:Microscopy, Electron; D008871:Microvilli / Q000201:enzymology; D009093:Mucus / Q000201:enzymology; D013649:Taste / Q000502:physiology*; D013650:Taste Buds / Q000201:enzymology* / Q000648:ultrastructure; D014426:Turtles / Q000378:metabolism*
D015105:3',5'-Cyclic-AMP Phosphodiesterases
Tsitokhimicheskoe vydelenie fosfodiésterazy tsiklicheskikh nucleotidov vo vkusovykh lukovitsakh cherepakhi Testudo horsfieldi pri vozdeĭstvii vkusovymi veshchestvami.