Concanavalin A induces an intraluminal alkalinization of thymocyte membrane vesicles.
Weak acid distribution methods demonstrate that mitogenic levels of concanavalin A induce an intravesicular alkalinization of isolated thymocyte membrane vesicles. Experiments with chemical reagents that crosslink the high affinity concanavalin A receptor and extensive correlation with known cellular events suggest that a "membrane Bohr effect" may participate in the initiation of mitogenesis.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003208:Concanavalin A / Q000378:metabolism / Q000494:pharmacology*; D004114:Dimethadione / Q000378:metabolism; D005976:Glutaral / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008744:Methylamines / Q000378:metabolism; D010504:Periodic Acid / Q000494:pharmacology; D011817:Rabbits; D013601:T-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure
D008744:Methylamines; D010504:Periodic Acid; D003208:Concanavalin A; D004114:Dimethadione; D005976:Glutaral