Morphochemical investigations of the liver and biochemical studies of the blood of guinea pigs in anaphylactic shock.
The experimental investigations have been carried out on 116 guinea pigs divided in two groups: the experimental and control group. The animals of the experimental group were sensitized with a 25% egg white suspension in 0.86% conc. of NaCl applied subcutaneously. After 21 days the same animals were exposed to the action of the same antigen in aerosol according to the method of Gerszanowicz, [16]. It has been shown, that in anaphylactic shock (acute and chronic) the damage of the lysosomal membranes in hepatocytes appeared which may be the cause of liberation among others also of acid phosphatase from the liver into the blood. Histochemically it was found a low phosphorylase and glucose-6-phosphatase activity, which was the basis of the assumption, that in anaphylactic shock we have to do with an enzymatic block--phosphorylase kinase--phosphorylase and inhibition of the enzymatic activity of glucose-6-phosphatase in the liver of guinea pigs. The comparison of the histochemical and biochemical results concerned with the amount of lipids, glycogen and nucleic acids in the liver revealed that the increasing amount of lipids is paralleled by decrease of glycogen. Among nucleic acids a growing level of ribonucleic acid was found while the level of the desoxyribonucleic acid remained stable.
D000135:Acid Phosphatase / Q000032:analysis; D000707:Anaphylaxis / Q000201:enzymology / Q000473:pathology*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006168:Guinea Pigs; D006651:Histocytochemistry; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008112:Liver Glycogen / Q000032:analysis; D008297:Male; D009696:Nucleic Acids / Q000032:analysis
D001798:Blood Proteins; D008055:Lipids; D008112:Liver Glycogen; D009696:Nucleic Acids; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase