title: Early events after infection of Escherichia coli by bacteriophage T5. III. Inhibition of uracil-DNA glycosylase activity.
abstract: The activity of uracil-DNA glycosylase in Escherichia coli decreases dramatically to less than 10% of its original level after infection of the cells by phage T5. Phage-induced protein synthesis is required for this inhibition to occur, and the inhibition is induced by a mutant capable of injecting only the first 8% of its DNA. The inhibitor activity in extracts of infected cells is heat labile and nondialyzable, and will inhibit enzyme activity present in extracts of uninfected cells.
mesh_terms: D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D045647:DNA Glycosylases; D003854:Deoxyribonucleotides / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor); D013604:T-Phages / Q000201:enzymology / Q000254:growth & development*; D051981:Uracil-DNA Glycosidase; D014779:Virus Replication
chemical_list: D003854:Deoxyribonucleotides; D002701:Chloramphenicol; D010770:Phosphotransferases; D017856:Phosphotransferases (Phosphate Group Acceptor); C020015:(deoxy)nucleoside-phosphate kinase; D009708:Nucleotidases; C020014:deoxynucleoside-5'-monophosphatase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase