title: Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis.
abstract: The phosphodiesterase from calf spleen (EC 3.1.4.18) was immobilized on several supports. Some properties of the most suitable enzyme support system--calf spleen phosphodiesterase bound to agarose-Concanavalin A--were investigated, e.g., pH dependence, influence of ionic strength of the buffer medium, and Zn2+-ion inhibition. The immobilized spleen phosphodiesterase showed about 60% of the activity of the free enzyme; the activity toward several oligonucleotide test substrates was unchanged for two months.
mesh_terms: D000818:Animals; D002417:Cattle; D003208:Concanavalin A; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D009841:Oligonucleotides / Q000032:analysis*; D010727:Phosphoric Diester Hydrolases; D011487:Protein Conformation; D012685:Sepharose; D013154:Spleen / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D009841:Oligonucleotides; D003208:Concanavalin A; D012685:Sepharose; D010727:Phosphoric Diester Hydrolases