title: Estimation of genetic variation at the DNA level from restriction endonuclease data.
abstract: We consider the estimation of the genetic variation in a natural population when the data are obtained by the use of restriction endonucleases. Under the restriction endonuclease technique, a particular DNA segment is considered and cut wherever a recognition sequence appropriate to the endonuclease occurs. We consider data generated when a random sample of homologous DNA segments is treated in this way with one or a battery of restriction endonucleases. The numbers and sizes of the fragments that result indicate the locations and the frequencies of the recognition sequence (or, with a battery of restriction endonucleases, of each recognition sequence). These frequencies in the sample form the basis for an estimate of the amount of genetic variation in the population.
mesh_terms: D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D014644:Genetic Variation; D005914:Globins / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D008970:Molecular Weight; D011110:Polymorphism, Genetic
chemical_list: D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes