Culture of chondrocytes in medium supplemented with fetal calf serum or a serum substitute: Ultroser G.
Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage-like differentiation i.e. the presence of type II collagen. Proliferation was also compared with another serum substitute, Nu-Serum. Ultroser G is shown to be equivalent to fetal calf serum as far as chondrocyte proliferation is concerned, clonal growth is improved and biosynthesis of type II collagen is maintained in primary culture.
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D002356:Cartilage / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003470:Culture Media / Q000494:pharmacology*; D005434:Flow Cytometry; D006133:Growth Substances / Q000097:blood; D009930:Organic Chemicals; D011817:Rabbits
D001802:Blood Substitutes; D003470:Culture Media; D006133:Growth Substances; D009930:Organic Chemicals; C044609:Ultroser G; D003094:Collagen