Effects of beta adrenergic agents and prostaglandin E1 on erythroid colony (CFU-E) growth and cyclic AMP formation in Friend erythroleukemic cells.
The formation of erythroid colonies from bone marrow and spleen cells infected with the polycythemic strain of the Friend virus (FV-P) was characterized in an in vitro methyl cellulose colony-forming system in response to prostaglandin E1 and the beta-2 adrenergic agonist, albuterol. Both drugs markedly inhibited the formation of CFU-E colonies of FV-P-infected bone marrow and spleen in the absence or presence of erythropoietin. The albuterol-mediated inhibition of CFU-E colonies (FV-P-infected) was selectively blocked by butoxamine, a beta-2 antagonist. Adenylate cyclase (AC) activity was also determined in FV-P spleen membrane preparations in response to albuterol and PGE1. Both agents stimulated enzyme activity, and butoxamine blocked the stimulation seen with albuterol. The ability of albuterol and PGE1 to stimulate AC activity in the FV-P-infected cells suggests that the effects of these agents on CFU-E formation may be mediated by specific beta-2 adrenergic and PG receptors through the adenylate cyclase-cyclic AMP system.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D004920:Erythropoiesis / Q000187:drug effects*; D005260:Female; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D011458:Prostaglandins E / Q000494:pharmacology*; D013154:Spleen / Q000473:pathology
D011458:Prostaglandins E; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000420:Albuterol