Synthesis of a model promoter for gene expression in Escherichia coli.
We have synthesized a 67-membered double-stranded DNA containing putative initiation sites for transcription and translation in E. coli. Among the potential applications of this DNA are construction of artificial genes, cloning of foreign genes in E. coli, and study of structure - activity relations in promoter and ribosome binding sites.
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000138:chemical synthesis* / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D007202:Indicators and Reagents; D008722:Methods; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D007202:Indicators and Reagents; D004262:DNA Restriction Enzymes