Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture.
The propagation of feline infectious peritonitis virus (NW1-FIPV strain) in cell culture is described. Tissue culture-propagated virus was used to inoculate specific-pathogen-free kittens intraperitoneally, intratracheally, or orally. Intraperitoneal inoculation caused seroconversion and effusive peritonitis in 100% of the kittens. Intratracheal inoculation produced disease in 60% of the kittens, and oral inoculation in only 20%. Seroconversions without production of disease occurred in 10% of the kittens inoculated by either the intratracheal or the oral route. The remainder of the kittens inoculated by the intratracheal (30%) and oral (70%) routes did not develop serum antibodies or disease.
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002371:Cat Diseases / Q000209:etiology* / Q000276:immunology; D002415:Cats; D002478:Cells, Cultured; D003332:Coronaviridae / Q000254:growth & development* / Q000276:immunology; D003333:Coronaviridae Infections / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D007267:Injections; D007274:Injections, Intraperitoneal; D010538:Peritonitis / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D014132:Trachea
D000914:Antibodies, Viral