[Biological method of blood plasma glucagon determination].
Biological method of glucagon determination in the blood plasma was worked out. The method consists in incubation of the rat liver membranes with glucagon which stimulates adenylcyclase, whose activity was determined by the accretion of cAMP content in the incubation medium. Membranes with increased sensitivity of glucagon were obtained; hormone concentrations in the plasma of healthy persons and of patients suffering from diabetes were determined. In healthy persons glucagon content varied from 54 to 175 pg/ml.
D000262:Adenylyl Cyclases / Q000096:biosynthesis; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D003920:Diabetes Mellitus / Q000097:blood; D005934:Glucagon / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology; D051381:Rats
D005934:Glucagon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases
Biologicheskiĭ metod opredeleniia gliukagona v plazme krovi.