In vitro construction of a B-tropic virus by recombination: B-tropism is a cryptic phenotype of xenotropic murine retroviruses.
A B-tropic virus was isolated in vitro from the progeny of mouse cells doubly infected with N-tropic and xenotropic murine leukemia viruses. Biological and structural evidence is presented suggesting that the phenotypically silent structural marker for B-tropism, expressed by the xenotropic virus p30, was transferred to an N-ecotropic virus via recombination, thus resulting in the expression of a B-ecotropic murine leukemia virus.
D000818:Animals; D002478:Cells, Cultured; D006023:Glycoproteins / Q000502:physiology; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000254:growth & development*; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000502:physiology; D014779:Virus Replication
D006023:Glycoproteins; D010446:Peptide Fragments; D014764:Viral Proteins