Ca-activated neutral protease and its inhibitors: in vitro effect on intact myofibrils.
SDS gel electrophoresis of troponin and myosin extracted from chicken myofibrils pretreated with Ca-activated neutral protease showed a pattern similar to that observed in the muscles of patients with Duchenne muscular dystrophy, that is, a decrease in troponin-I and troponin-C with relative preservation of troponin-T and degradation of the heavy chain of myosin. Also, alpha-actinin and troponin-C were released from myofibrils. Two inhibitors of Ca-activated neutral protease, leupeptin and E-64, inhibited the degradative action of Ca-activated neutral protease on intact myofibrils in vitro.
D000376:Agmatine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002154:Calpain; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology; D009210:Myofibrils / Q000187:drug effects*; D009218:Myosins / Q000032:analysis; D010447:Peptide Hydrolases / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D014336:Troponin / Q000032:analysis
D007976:Leupeptins; D011480:Protease Inhibitors; D014336:Troponin; D000376:Agmatine; D010450:Endopeptidases; D010447:Peptide Hydrolases; D002154:Calpain; D009218:Myosins; D007930:Leucine; C024974:E 64; D002118:Calcium