Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.
Nuclei isolated from herpes simplex virus (HSV)-infected KB cells actively synthesize HSV RNA in vitro; the RNA can be hybridized with HSV DNA or nuclear RNA from HSV-infected cells. Nascent RNA molecules labeled in vivo with 32PO4 were elongated, utilizing the nuclear system to incorporate Hg-CTP at their 3' ends, and then isolated on an affinity column. Hybridization of isolated nascent RNA molecules showed that greater than 50% of them were HSV specific and that more than 25% were self-complementary.
D002277:Carcinoma; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D002846:Chromatography, Affinity; D006801:Humans; D009062:Mouth Neoplasms; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis*; D018139:Simplexvirus / Q000254:growth & development / Q000378:metabolism*; D014779:Virus Replication
D012367:RNA, Viral