Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.
A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.
D003902:Detergents; D005233:Fatty Alcohols / Q000494:pharmacology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008828:Microbiological Techniques; D012087:Reoviridae / Q000302:isolation & purification*; D012401:Rotavirus / Q000145:classification / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate
D003902:Detergents; D005233:Fatty Alcohols; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate