title: The gene-enzyme relationships of proline biosynthesis in Escherichia coli.
abstract: A simple chromatographic procedure has been devised to separate gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase, allowing the measurement of the former in crude Escherichia coli extracts. Analysis of a number of strains of E. coli has demonstrated that gene proA codes for gamma-glutamyl phosphate reductase and proB for gamma-glutamyl kinase. Introduction of a ColE1 hybrid plasmid containing the proA,B region into a strain with a chromosomal deletion of proA,B led to 3- and 17-fold increases in the specific activities of gamma-glutamyl kinase and gamma-glutamyl phosphate reductase, respectively.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005838:Genotype; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D009154:Mutation; D010770:Phosphotransferases / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor); D010957:Plasmids; D011392:Proline / Q000096:biosynthesis*; D011762:Pyrroline Carboxylate Reductases / Q000378:metabolism
chemical_list: D011392:Proline; D000445:Aldehyde Oxidoreductases; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D011762:Pyrroline Carboxylate Reductases; D010770:Phosphotransferases; D017851:Phosphotransferases (Carboxyl Group Acceptor); C020650:glutamate 5-kinase