title: Galactosamine is an inducer of Gal genes in Saccharomyces cerevisiae.
abstract: In yeast cells galactosamine in concentrations of 0.1 1M partially inhibits the synthesis of RNA but has little effect on the protein synthesis. In vivo and in vitro studies show that galactosamine is metabolized in yeast to UDP-N-acetylhexosamines but at a reduced rate, compared to the metabolism of galactose. The addition of galactosamine to growing yeast cells leads to the induction of the galactose pathway enzymes. Studies using different mutants in the galactose genes provide evidence that galactosamine is an inducer of the galactose structural genes in yeast. The same degree of induction of galactokinase and galactotransferase, found when galactose or galactosamine were used as inducers, supports the model of coordinated regulation in the expression of the structural genes for the galactose pathway enzymes in yeast.
mesh_terms: D002238:Carbohydrate Epimerases / Q000096:biosynthesis*; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis; D005686:Galactokinase / Q000096:biosynthesis* / Q000235:genetics; D005688:Galactosamine / Q000494:pharmacology*; D005690:Galactose / Q000494:pharmacology; D005796:Genes / Q000187:drug effects*; D009713:Nucleotidyltransferases / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014534:UDPglucose 4-Epimerase / Q000096:biosynthesis* / Q000235:genetics; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D012313:RNA; D005688:Galactosamine; D005686:Galactokinase; D009713:Nucleotidyltransferases; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase; D002238:Carbohydrate Epimerases; D014534:UDPglucose 4-Epimerase; D005690:Galactose