[Effect of surface-active agents on Na, K-ATPase from guinea pig kidneys].
The effect of surface active agents on the activity of Na, K-ATPase and on the direct medium 18O exchange in the membrane preparations of the guinea pig kidney has been studied. The medium 18O exchange was considered as a character of the K+-dependent stage in ATPase reaction. Low concentration of all the surface active agents were shown to stimulate, and high concentrations -- to inhibit both ATPase and medium 18O exchange. The relation between medium 18O exchange and Na,K-ATPase activity was found to be equal to 1.5 +/- 0.1. The treatment of preparations by activating amounts of the surface active agents resulted in lowering this relation up to 1.0 +/- 0.09 and 1.3 +/- 0.04 for DOC and triton X-100, correspondently, due to a stronger stimulation of ATPase activity than of medium 18O exchange. With the inhibiting amounts of triton X-100 and histone H2a, this relation did not change, but it decreased in the presence of equal amounts of DOC.
D000818:Animals; D003840:Deoxycholic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D006657:Histones / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D008565:Membrane Proteins / Q000378:metabolism; D010103:Oxygen Isotopes; D011092:Polyethylene Glycols / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D006657:Histones; D008565:Membrane Proteins; D010103:Oxygen Isotopes; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D000254:Sodium-Potassium-Exchanging ATPase
Vliianie poverkhnostno-aktivnykh veshchestv na Na, K-ATFazu iz pochek morskoĭ svinki.