title: The K+-and HCO3-stimulated phosphatase activities in renal microsomes of rats.
abstract: The K+-stimulated phosphatase activity of microsomes from rat kidney was not inhibited by L-phenylalanine, but the HCO3-stimulated phosphatase activity was markedly inhibited by L-phenylalanine. Valinomycin enhanced the HCO3-stimulated phosphatase activity, but did not enhance the K+-stimulated phosphatase activity. Ouabain did not inhibit the HCO3-stimulated phosphatase activity, but inhibited the K+-stimulated phosphatase activity. The renal K+-stimulated phosphatase activity was suppressed to 40% of the control values by adrenalectomy, but the renal HCO3-stimulated phosphatase activity was little suppressed by adrenalectomy. The renal K+-stimulated phosphatase activity in intact and adrenalectomized rats was found to be significantly elevated, in a manner similar to the elevation of the renal (Na+ + K+)-ATPase activity by aldosterone treatment (P less than 0.02).
mesh_terms: D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000315:Adrenalectomy; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D002414:Cations, Monovalent / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D008168:Lung / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D001639:Bicarbonates; D002414:Cations, Monovalent; D014634:Valinomycin; D010649:Phenylalanine; D010042:Ouabain; D012964:Sodium; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D008274:Magnesium; D011188:Potassium