Assessment of cell-mediated immunity against coxsackievirus B3-induced myocarditis in a primate model (Papio papio).
Delayed hypersensitivity and myocarditis were induced in five baboons (Papio papio) after inoculation with myocarditic murine coxsackievirus B3 (CVB3m). By means of the [3H]thymidine incorporation assay and the agarose droplet cell migration inhibition assay, specific cell-mediated immunity was detected in all five animals against viral antigens and/or KCl-extractable soluble antigens extracted from the heart of a CVB3m-inoculated baboon. KCl-extracted antigens from normal baboon heart tissues, as well as KCl-extracted antigens from spleen or liver tissues from a CVB3m-inoculated baboon, failed to stimulate baboon peripheral blood lymphocytes or inhibit the migration of baboon peritoneal exudate cells. The appearance of dissimilar antigen(s) in extracts of heart tissues from CVB3m-inoculated baboons which did not contain infectious CVB3m parallels the appearance of novel KCl-extractable antigen(s) induced by CVB3m in murine heart tissues (Paque et al., J. Immunol. 120:1672-1678). The animal model described represents the first systematic evaluation of cell-mediated immunity in CVB3m-induced myocarditis in primates and suggests that a similar phenomenon may occur in humans.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002464:Cell Migration Inhibition; D003384:Coxsackievirus Infections / Q000276:immunology*; D004195:Disease Models, Animal; D029822:Enterovirus B, Human / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D009205:Myocarditis / Q000209:etiology* / Q000276:immunology; D009500:Neutralization Tests; D010215:Papio
D000914:Antibodies, Viral