title: Requirements for growth of neurites from embryonic chick cerebellum in culture.
abstract: Embryonic chick cerebella were cut into pieces and cultured under various conditions. Neurites were extended actively to collagen gel for a week or so and then degenerated. The neurite growth for the first two days was similar, also in the serum-free medium. Among 15 substances tested, insulin and cyclic AMP facilitated the growth.
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002531:Cerebellum / Q000166:cytology*; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D000242:Cyclic AMP / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology; D005728:Ganglia, Sympathetic / Q000166:cytology; D007328:Insulin / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects; D009474:Neurons / Q000166:cytology
chemical_list: D003470:Culture Media; D007328:Insulin; D009414:Nerve Growth Factors; D000242:Cyclic AMP