A generalized technique for deletion of specific genes in large genomes: alpha gene 22 of herpes simplex virus 1 is not essential for growth.
We describe a general method for inactivation and deletion of genes at specific sites in large DNA genomes. In the first step of the procedure, the herpes simplex virus thymidine kinase is inserted into the genome at a specific site. In the second step, the thymidine kinase gene is desired sequences flanking the insertion site are deleted. Both steps involve recombination of the genomes with cloned chimeric fragments and utilize the available selection for or against thymidine kinase to select the desired genomes. We have applied the procedure to inactivate and to delete portions of an alpha gene of herpes simplex virus 1 specifying protein 22. The recombinant virus carrying the thymidine kinase inserted into the gene 22 and viruses exhibiting 0.1 kb and 0.7 kb deletions in the gene 22 specify new alpha polypeptides with molecular weights approximately 30% of the wild-type gene 22 product and grown normally in Vero cell cultures.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004274:DNA, Recombinant; D005814:Genes, Viral; D005821:Genetic Techniques; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis
D004274:DNA, Recombinant; D014764:Viral Proteins; D013937:Thymidine Kinase