Studies on the resolution of cytochrome oxidase.
Cytochrome oxidase has been resolved in acetic acid and high salt/detergent media. In 0.5% acetic acid, the smaller subunits of the enzyme are selectively extracted with retention of an insoluble protein fraction containing subunits I-IV, VII. This fraction retains all the heme and copper of the original enzyme in a spectrally unaltered state, and possesses enzymic activity comparable to the unresolved enzyme. The further removal of subunit IV from this fraction results in migration of heme and copper and modification of their spectral characteristics. Resolution of the enzyme in a high salt/detergent medium extracts smaller subunits (V-VII) together with subunit IV and some heme and copper. The heme associated with this enzymically active extract has spectral characteristics that are partially suggestive of heme a3. It is suggested that the fraction of subunits I-IV,VII, resolved in dilute acetic acid, may represent the limit of resolution of the cytochrome oxidase complex that remains actively and spectrally indistinguishable from the original enzyme.
D000085:Acetates; D000818:Animals; D002417:Cattle; D003300:Copper / Q000032:analysis; D003902:Detergents; D003576:Electron Transport Complex IV / Q000378:metabolism; D006418:Heme / Q000032:analysis; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology; D017830:Octoxynol; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000032:analysis; D011092:Polyethylene Glycols; D012492:Salts; D013053:Spectrophotometry
D000085:Acetates; D003902:Detergents; D046911:Macromolecular Substances; D010743:Phospholipids; D012492:Salts; D011092:Polyethylene Glycols; D006418:Heme; D003300:Copper; D017830:Octoxynol; D003576:Electron Transport Complex IV