Transposition of Tn951 (Tnlac) and cointegrate formation are thermosensitive processes.
The frequency of transposition of Tnlac to pGC200, an IncFII R plasmid, increased during the storage of the host strain. This result is explained by the fact that the transpositional event is temperature-dependent: it occurred readily when the host strain was grown at 30 degrees C but it was nearly undetectable when the host strain was grown and kept at 37 degrees C. Fusions between two different plasmids carrying Tnlac with pGC200 were also thermosensitive, suggesting a relation between cointegrate formation and transposition. Lactose did not influence the frequency of transposition of Tnlac.
D004247:DNA; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D007763:Lac Operon; D009692:Nucleic Acid Heteroduplexes; D010957:Plasmids; D015007:Yersinia / Q000235:genetics
D004251:DNA Transposable Elements; D009692:Nucleic Acid Heteroduplexes; D004247:DNA