Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
D000096:Acetone; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D008832:Microchemistry; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology; D011506:Proteins / Q000302:isolation & purification*; D012808:Sigma Factor / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D014157:Transcription Factors / Q000302:isolation & purification*
D011506:Proteins; D012808:Sigma Factor; D014157:Transcription Factors; D000096:Acetone; D012967:Sodium Dodecyl Sulfate; D012321:DNA-Directed RNA Polymerases; D004264:DNA Topoisomerases, Type I