Enzyme-linked immunosorbent assay for detection of poliovirus antigen and antibody.
A sensitive method is described to detect poliovirus antigen in diluted samples of semi-processed and processed Salk vaccine material using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The introduction of a simple conjugate-blocking step permits confirmation of antigen specificity and allows evaluation of neutralising antibody concentration in the sera employed as soon as four hours after sample addition.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011054:Poliovirus Vaccine, Inactivated