title: The gene for polypeptide IX of human adenovirus type 7.
abstract: This paper describes the nucleotide sequence of subgroup B human adenovirus type 7 (Ad7) between positions 3351-4010, and of cloned cDNA derived from mRNAs encoded by this segment. One of these (mRNA VII) is shown to be unspliced, and has its 5'- and 3'-ends encoded by positions 3460 (determined by the nuclease S1 technique) and 3939, respectively. The mRNA sequence contains a single open reading frame for protein biosynthesis between the first available initiation triplet AUG at position 3481 to the stop codon UAA at position 3895. It can specify a polypeptide of 138 amino acids (14 098 daltons) which must be polypeptide IX of Ad7, as is evident from its mapping position, and from a comparison with the sequence of the protein IX gene of subgroup C adenovirus type 5.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D014764:Viral Proteins; D011061:Poly A; D004247:DNA; D004262:DNA Restriction Enzymes