Partial purification and characterization of a triacylglycerol-transfer protein from rabbit serum.
Rabbit lipoprotein-free serum was found to contain a protein fraction that mediates the transfer of [3H]triacylglycerol from low-density lipoprotein to high-density lipoprotein. Fractionation of rabbit lipoprotein-free serum by DEAE-Sephadex chromatography, ammonium sulphate precipitation, concanavalin-A-Sepharose chromatography, Sephadex G-200 gel filtration, phenyl-Sepharose chromatography and Sephadex G-100 gel filtration, yielded a preparation that had a 500-fold increase in transfer activity compared to that of the starting sample. The transfer activity appeared to reside in a glycoprotein of molecular weight in the range 100 000-155 000 and an isoelectric point at pH 9.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D011817:Rabbits; D014280:Triglycerides / Q000097:blood*
D002352:Carrier Proteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides; D002731:4-Chloromercuribenzenesulfonate