title: Proteolytic activation of Ca2+-dependent cyclic nucleotide phosphodiesterase from Escherichia coli.
abstract: Ca2+ -dependent cyclic nucleotide phosphodiesterase from Escherichia coli was activated by limited proteolysis. The proteolytically activated enzyme lost the sensitivity to Ca2+ but its kinetic properties were not altered.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000302:isolation & purification / Q000378:metabolism*; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000302:isolation & purification / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology
chemical_list: D010664:Phenylmethylsulfonyl Fluoride; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D002118:Calcium