[DNA immobilization on modified inorganic sorbents].
Fragments of denaturated DNA having the length of about 500 nucleotides were immobilized, using silica carriers modified by organic polymers. It was shown that the organic coating allows to obtain sorbents, which practically exclude non-specific sorption with respect to DNA. The immobilization was carried out on hydroxyl-containing sorbents and on their phrosphorylated derivatives by means of water-soluble carbodiimide. The resulting preparations contained up to 60 units at A260 of DNA per 1 g of carrier. The effects of endonuclease A236, exonuclease A5 and pancreatic DNAse on the immobilized RNA were studied.
D000327:Adsorption; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D004262:DNA Restriction Enzymes; D003851:Deoxyribonucleases; D004720:Endonucleases; D007527:Isoenzymes; D009691:Nucleic Acid Denaturation; D010179:Pancreas / Q000201:enzymology; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011108:Polymers; D013329:Structure-Activity Relationship
D007527:Isoenzymes; D011108:Polymers; D004247:DNA; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; C027271:endonuclease A236; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I
Immobilizatsiia DNK na modifitsirovannykh neorganicheskikh sorbentakh.