Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11.
Poly(A)-containing mRNA from mouse myeloma MPC11 was transcribed into cDNA which was cloned in the PstI site of the plasmid pBR322. The transformants were screened by hybridization with a cDNA fragment, derived from plasmid p gamma(11)7, corresponding to the 5' portion of the constant region of MPC11 heavy chain. Several positive transformants were found to contain various lengths of the variable region of the heavy chain. We describe the structure and sequence of one of these clones, pV(11)2, which contains cDNA corresponding to the entire variable region of MPC11 heavy chain and extends to codon 248 in the constant region. The protein sequence deduced from the DNA sequence indicates that the variable region of MPC11 heavy chain contains 121 amino acids and belongs to subgroup II of mouse heavy chains. Comparison of this sequence with other heavy chain sequences suggests a J (joining) segment of 16 residues which overlaps five residues of the third hypervariable region. The cDNA sequence shows that there is no discontinuity between the end of the variable region and the beginning of the constant region.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005815:Genetic Code; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010954:Plasmacytoma; D011061:Poly A / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger; D011061:Poly A; D004262:DNA Restriction Enzymes