title: Characteristics of histamine release evoked by acetylcholine in isolated rat mast cells.
abstract: 1. Histamine secretion from rat mast cells occurs in the presence of nanomolar concentrations of acetylcholine. 2. Intact glycolytic and oxidative metabolism is required for the acetylcholine-induced histamine secretion. Removal of extracellular glucose, hypoxia, cyanide and monoiodoacetate almost completely inhibit the secretion. 3. The secretion of histamine is dependent on the extracellular H ion concentration and is blocked when the cells are exposed to Na-deficient media. 4. The order of potency of cholinrgic agonists in evoking the secretion of histamine is oxotremorine > acetylcholine > choline > carbamycholine > nicotine. 5. Atropine competitively blocks the acetylcholine-induced histamine secretion, indicating the presence of cholinergic muscarinic receptors on mast cells. 6. Dibutyryl cyclic AMP and adrenaline inhibit the acetylcholine-induced histamine secretion, indicating a regulatory function afforded by cyclic nucleotides in the cholinergic histamine release.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006636:Histamine Release; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D010095:Oxotremorine / Q000494:pharmacology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D012964:Sodium / Q000494:pharmacology
chemical_list: D010095:Oxotremorine; D003994:Bucladesine; D001285:Atropine; D002217:Carbachol; D012964:Sodium; D002794:Choline; D000109:Acetylcholine; D004837:Epinephrine