Serological evaluation of human brain tumors using a microradioisotopic staphylococcal protein A assay.
A microradioisotopic staphylococcal protein A assay was applied to the serological evaluation of preoperative sera from brain tumor patients. The assay was performed in Terasaki plates, and a stainless steel punch and multiholed die facilitated sample prearation. Using allogeneic astrocytoma target cells, 15/19 of the brain tumor patients' sera were reactive, compared to 1/21 controls 0/7 nonmalignant brain disease patients' sera were positive, and 5/20 sera from patients with epithelial malignancies. The brain tumor sera were not reactive against a rectal cancer cell line.
D001932:Brain Neoplasms / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D005909:Glioblastoma / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D013205:Staphylococcal Protein A / Q000276:immunology*
D007202:Indicators and Reagents; D013205:Staphylococcal Protein A