title: Synthesis and transport of the precursor for the beta-subunit of rat liver F1-ATPase.
abstract: The synthesis and intracellular transport of the beta-subunit of rat liver F1-ATPase was studied in a cell-free system, using free polysomal mRNA from rat liver and isolated rat hepatocytes. The beta-subunit of rat liver F1-ATPase is synthesized as a larger precursor form in rabbit reticulocyte lysate and then transported into isolated mitochondria in the absence of protein synthesis. In pulse experiments at 37 degrees C, the precursor of the beta-subunit reached a plateau 30 min after the pulse. The labeled mature beta-subunit appeared in the particulate fraction (containing mitochondria) after a time lag and increased almost linearly with time up to 40 min.
mesh_terms: D000818:Animals; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000378:metabolism*; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004792:Enzyme Precursors; D006180:Proton-Translocating ATPases