[Electron-acceptor (acid) properties of the contents of blood platelet intracellular granules and their significance for the reactions of these cells].
The fluorescent marker method and measurements of aggregation were utilized to study the significance of the acidic intragranular space in blood platelets. Acridine orange is liberated from 5-HT granules after alkalinization by the ammonia ion which inhibits the induction of platelet aggregation by ADP and causes the platelet disaggregation. At low doses, ammonia ion enhances ADP-induced aggregation. The transformation kinetics of 5-HT granules was investigated in the course of clot formation with a new instrument that simultaneously monitors the light transmittance (aggregation, clot formation) and fluorescence intensity (accumulation and liberation of fluorescent marker). These experiments provide the evidence that processes of fibrin filament sedimentation and fibrin-blood platelet interaction were followed by a liberation of fluorescent marker from 5-HT granules.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D002479:Inclusion Bodies / Q000378:metabolism*; D009940:Organoids / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism
D012701:Serotonin
Elektronno-aktseptornye (kislotnye) svoĭstva soderzimogo vnutrikletochnykh granul krovianykh plastinok i ikh znachenie dlia nekotorykh reaktsiĭ étikh kletok.