Two-hit kinetics of focus formation in cells transfected with Rous sarcoma provirus.
DNA was purified from an established line of SR-E-transformed quail cells and was assayed for transfection in turkey cells. Transfection events were determined from foci of transformed cells growth under agar overlay which was used to prevent the spread of the progeny virus. It was found that transfection with Rous sarcoma proviruses follows two-hit kinetics, indicating that at least two (unlinked) provirus are required to give rise to a focus of transformed cells.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D007700:Kinetics; D011784:Quail; D014162:Transfection; D014422:Turkeys
D004279:DNA, Viral