Effect of the time interval between blood sampling and assay on serum angiotensin I-converting enzyme activity from captopril-treated rats.
Intra-arterial injection of captopril (1 mg/kg) effectively lowered arterial blood pressure in aorta-coarcted hypertensive rats along with an associated reduction of serum angiotensin I-converting enzyme (ACE) activity. ACE activity in serum samples from captopril-treated animals that were assayed within 60 min after collection was inhibited 93%. However, this inhibition progressively decreased as the interval between time of assay and blood collection increased. This information would appear to be of considerable value in planning experiments for the determination of serum ACE activity from captopril-treated animals.
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D001012:Aorta, Abdominal / Q000502:physiology; D001800:Blood Specimen Collection; D002216:Captopril / Q000494:pharmacology*; D006973:Hypertension / Q000378:metabolism; D008026:Ligation; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000097:blood; D011392:Proline / Q000031:analogs & derivatives*; D051381:Rats; D013997:Time Factors
D000806:Angiotensin-Converting Enzyme Inhibitors; D011392:Proline; D002216:Captopril; D007703:Peptidyl-Dipeptidase A