Subcellular location of two enzymes involved in the synthesis of phosphorylated recognition markers in lysosomal enzymes.
Phosphorylated recognition markers in lysosomal enzyme appear to be synthesized by transfer of alpha-N-acetylglucosamine 1-phosphate groups to C6 hydroxyl of mannose residues in glycosylated enzyme precursors and a subsequent hydrolysis from the diester groups of the N-acetylglucosamine residues. The transfer and the diesterase activities were studied in subcellular fractions of rat liver. Both activities fractionated like the Golgi marker galactosyltransferase.
D000818:Animals; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D017351:N-Acetylglucosaminyltransferases; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D006023:Glycoproteins; D014537:Uridine Diphosphate N-Acetylglucosamine; D005700:Galactosyltransferases; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C023224:alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; D005952:Glucose-6-Phosphatase; D010727:Phosphoric Diester Hydrolases; C026645:N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase