title: Angiotensin converting enzyme in cultured endothelial cells and growth medium. Relationships to enzyme from kidney and plasma.
abstract: We have previously reported that cultured cells from swine aorta possess angiotensin converting enzyme (peptidyldipeptide hydrolase, EC 3.4.15.1) and release it into serum-free culture medium. The present work compares enzyme from these two sources, and from swine kidney and serum, with respect to antibody and lectin binding. Purified enzyme from swine kidney, and the activity in swine serum, cultured endothelial cells and culture medium bind similarly to rabbit antibodies prepared against the kidney converting enzyme. Enzyme from each of these sources was allowed to bind to an immobilized lectin (Ricinus communis), which binds to terminal galactose residues of glycoproteins. Increasing concentrations of galactose were used to remove enzyme from the lectin column and the distribution of enzyme activity in the galactose eluates was determined. The elution pattern was similar for kidney and endothelial cell enzyme, and different from the pattern found for both serum and medium enzymes. Neuraminidase treatment of either serum or medium enzyme altered the distribution of activity eluted to that found for endothelial cell or kidney enzymes. The effects of neuraminidase suggest that the difference in lectin binding between cell and medium enzyme reflects differences in the number of terminal sialic acid residues that cover galactose residues.
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D000937:Antigen-Antibody Reactions; D001011:Aorta / Q000201:enzymology; D001808:Blood Vessels / Q000201:enzymology*; D002478:Cells, Cultured; D004727:Endothelium / Q000201:enzymology; D007668:Kidney / Q000201:enzymology*; D037102:Lectins / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D013552:Swine
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D037102:Lectins; D007703:Peptidyl-Dipeptidase A