Irreversible attachment of immunoglobulins and F(ab')2 fragments to their specific cell membrane antigens.
Antiserum was raised in sheep against rat kidney glomerular basement membrane, and the antibodies and their F(ab')2 fragments were prepared. These were reacted with one arm of the bifunctional reagent toluene diisocyanate at pH 7.5 and injected into the tail vein of rats. Kidney sections were taken from the rats and, after washing, incubated at pH 8.5 to permit the other arm of the toluene diisocyanate to become reactive. This procedure resulted in the specific irreversible attachment of the antibodies and their F(ab')2 fragments to their corresponding glomerular basement membrane antigens.
D000818:Animals; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D001666:Binding Sites, Antibody; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology; D051381:Rats; D012756:Sheep; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology
D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D014051:Toluene 2,4-Diisocyanate