Prolyl and lysyl hydroxylase activities of human skin fibroblasts: effect of donor age and ascorbate.
Prolyl and lysyl hydroxylase activities in cultures of human skin fibroblasts from fetal to 94-yr-old donors were measured. In contrast to earlier studies with whole skin, neither prolyl nor lysyl hydroxylase activity was found related to donor age. Prolyl hydroxylase activity increased 3- to 6-fold when cell extracts were incubated with ascorbate and other hydroxylation cofactors before assay. A similar increase in prolyl hydroxylase activity occurred when cells were incubated with ascorbate. Lysyl hydroxylase activity remained unaltered under these conditions.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D001205:Ascorbic Acid / Q000494:pharmacology*; D001206:Ascorbic Acid Deficiency / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006900:Hydroxylation; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011347:Procollagen / Q000096:biosynthesis; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000378:metabolism*; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*
D011347:Procollagen; D003094:Collagen; D006899:Mixed Function Oxygenases; D011393:Procollagen-Proline Dioxygenase; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D001205:Ascorbic Acid