Expression of a bacterial gene in mammalian cells.
Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D004926:Escherichia coli; D005796:Genes; D000882:Haplorhini; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007926:Lesch-Nyhan Syndrome / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014161:Transduction, Genetic; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase