title: Photoaffinity labeling of luteinizing hormone releasing hormone receptor of rat pituitary membrane preparations.
abstract: Specific LHRH receptor proteins of plasma membrane preparation from pituitary glands of the rat were identified using an 125I-labeled photoreactive LHRH derivative, [D-Lys6-N epsilon-azidobenzoyl]LHRH. This analog retained high binding affinity (apparent Kd value of 0.1 nM) to a single class of receptors. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated the specific photolabeling of two proteins, a major band with an apparent molecular weight of 60,000 daltons and a minor band of 48,000 daltons. The latter is probably a degradation product of the receptor.
mesh_terms: D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001386:Azides; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D011966:Receptors, LHRH
chemical_list: D000345:Affinity Labels; D001386:Azides; D011956:Receptors, Cell Surface; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; C031737:LHRH, N-epsilon-azidobenzoyl-Lys(6)-