[Isolation and characteristics of actin-like proteins in liver mitochondria].
Using affinity chromatography on DNAase I-Sepharose, an actin-like protein was isolated from rat liver mitochondria and purified 60-fold. SDS electrophoresis in polyacrylamide gel revealed that the protein migrated with muscle actin and thus had the molecular weight of 42 000 Da. Evidence for the actin-like nature of the mitochondrial protein could be obtained from the fact that the protein inhibited the activity of pancreatic DNAase I which, similar to the smooth muscle protein, was less conspicuous than that of its muscle counterpart. Unlike striated muscle actin but similar to the smooth muscle protein, the mitochondrial actin weakly stimulated the Mg-ATPase activity of rabbit skeletal muscle myosin. After manyfold washing of the mitochondria with isotonic isolation media, the content of the actin-like protein remained unchanged, which indirectly points to the presence of insignificant cytoplasmic actin contaminations. During isoelectrofocusing, the mitochondrial actin-like protein yielded two forms, i. e., beta- and gamma-isoactins, whose ratio was 8:1. The pI values for the beta- and gamma-isoforms were 5.52 and 5.59, respectively. The identical position of the absorption spectra (260 nm) and fluorescence excitation spectra (around 280 nm) maxima of the actin-like protein and smooth and skeletal muscle actins testify to their homology.
D000199:Actins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors; D007525:Isoelectric Focusing; D008930:Mitochondria, Liver / Q000378:metabolism*; D009130:Muscle, Smooth / Q000201:enzymology / Q000378:metabolism; D009132:Muscles / Q000201:enzymology / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D011817:Rabbits; D051381:Rats
D000199:Actins; D000255:Adenosine Triphosphate; D003850:Deoxyribonuclease I; D000251:Adenosine Triphosphatases; D009218:Myosins
Vydelenie i kharakteristika aktinopodobnogo belka mitokhondriĭ pecheni.