title: Two different intrachain cAMP binding sites of cAMP-dependent protein kinases.
abstract: The regulatory subunits of both isozymes of cAMP-dependent protein kinase bind 2 mol of cAMP/mol of monomer. cAMP dissociation studies indicate similar cAMP binding behavior for each isozyme. Each has two different intrachain cAMP binding components present in approximately equal amounts and the rate of cAMP dissociation is 5- to 10-fold slower from one site (Site 1) than from the other (Site 2). Equilibrium [3H]cAMP binding is inhibited by several competing cyclic nucleotides. Following equilibrium binding using saturating [3H]cAMP in the presence of competing nucleotide, the pattern of release of [3H]cAMP, monitored in the presence of an excess of nonradioactive cAMP, suggests site-specific selectivity of some of the cyclic nucleotides. As compared with cAMP, cIMP prefers Site 2 for both regulatory subunits, whereas N6, O2-dibutyryl-cAMP shows a similar preference only with isozyme II regulatory subunit. 8-Bromo-cAMP, 8-bromo-cGMP, and 8-azido-cAMP prefer Site 1 of both proteins. The results indicate that for each isozyme the two intrachain binding sites have different analogue specificities and cAMP dissociation rates. Site 1 or Site 2 of one isozyme has a similar but not identical cyclic nucleotide specificity and cAMP dissociation rate to the corresponding site of the other isozyme.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D000242:Cyclic AMP; D007527:Isoenzymes / Q000378:metabolism; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D000242:Cyclic AMP; D011494:Protein Kinases