Molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type 1a DNA.
The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. The integrity and the homogeneity of the viral DNA thus obtained was confirmed by restriction endonucleases analysis. Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D008970:Molecular Weight; D027383:Papillomaviridae / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids
D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes