Tissue distribution, uptake, and requirement for alpha-tocopherol of rainbow trout (Salmo gairdneri) fed diets with a minimal content of unsaturated fatty acids.
The metabolism of and requirements for alpha-tocopherol in rainbow trout fed diets containing 1% linolenic acid as sole source of unsaturated fat and graded levels of tocopherol (0.06-10 mg/100 g) were examined. Fish grew 5-fold over a 16-week period. In liver, tocopherol was concentrated in mitochondria with little in cytosol. Orally administered [3H]-tocopherol was rapidly taken up by plasma and liver but uptake into erythrocytes and white muscle was much slower; in most tissues radioactivity reached a plateau after about 3 days but in red muscle radioactivity increased over a 10-day period. Activities of enzymes that prevent free radical initiated tissue damage did not change in tocopherol deficiency. Tocopherol-deficient trout had no gross or subcellular pathologies even though liver and muscle were severely depleted of the vitamin. Ascorbic acid-stimulated lipid peroxidation in liver organelles indicated a tocopherol requirement of 2-3 mg/100 g diet; the molar ratios of polyunsaturated fatty acids to tocopherol in livers of trout fed diets lacking or supplemented with tocopherol (100 mg/100 g) were 980 and 170, respectively.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D008042:Linolenic Acids / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism; D009751:Nutritional Requirements; D012487:Salmonidae / Q000378:metabolism*; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*; D014810:Vitamin E / Q000008:administration & dosage / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism; D017962:alpha-Linolenic Acid
D005231:Fatty Acids, Unsaturated; D008042:Linolenic Acids; D017962:alpha-Linolenic Acid; D014810:Vitamin E; D001205:Ascorbic Acid