Differential susceptibility of fibroblastic and non-fibroblastic surface fibronectins to periodate-borohydride labelling and enzymic treatment.
Surface labelling of liver epithelioid cells permits the detection of a 230,000 dalton component reactive with antifibronectin serum, when tritiated borohydride labelling is preceded by oxidation of sugar residues with galactose oxidase but not with sodium periodate. Similar experiments with liver fibroblasts lead to the labelling of a similar 230,000 dalton component both when sugar oxidation is carried out with galactose oxidase or periodate. Neuraminidase treatment prior to surface labelling lead to an increased labelling in both cell types, although a decreased labelling in the fibronectin region was apparent in the epithelioid cells.
D000818:Animals; D001894:Borohydrides; D002460:Cell Line; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005347:Fibroblasts / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis*; D005692:Galactose Oxidase / Q000494:pharmacology; D008970:Molecular Weight; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D051381:Rats; D014316:Tritium
D001894:Borohydrides; D005353:Fibronectins; D014316:Tritium; D010504:Periodic Acid; D005692:Galactose Oxidase; D009439:Neuraminidase