Distribution of high mobility group and other acid-soluble proteins in fractionated chromatin.
Chromatin was fractionated by digestion with deoxyribonuclease II and precipitation with MgCl2. The Mg2+-soluble fraction, known to be enriched in transcribed DNA sequences, was enriched also in high mobility group proteins 1 and 2 and contained almost all other acid-soluble nonhistone proteins.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002843:Chromatin / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D003851:Deoxyribonucleases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases; D004720:Endonucleases / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D015636:Magnesium Chloride; D012995:Solubility
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D015636:Magnesium Chloride; D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C019329:deoxyribonuclease II; D008274:Magnesium