Packaging of plasmid DNA containing the cohesive ends of coliphage lambda.
High yields of ColE1::Tn3-cos lambda plasmid genomes packaged in phage lambda virions (2.10(9) per ml) are produced by thermal induction of E. coli W3350 (lambda cI1857S7) lysogens carrying the plasmid DNA. The plasmid DNA is packaged in the linear form, with the right m' terminus of lambda being associated with the lambda tail.
D010582:Bacteriophage lambda / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010957:Plasmids
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral