The production and characterisation of products of the lipoxygenase enzyme system released by rat peritoneal macrophages.
Rat peritoneal monocytes and macrophages when exposed to the ionophore A23187 release products of the lipoxygenase pathway of arachidonic acid metabolism which cause the aggregation and chemokinesis of polymorphonuclear leucocytes suspensions. The major biologically active compound released was leukotriene B which accounted for greater than 80% of the activity. The remaining biological activity was due to the release of a more polar as yet unidentified compound. In addition rat macrophages release 5, 12 and 15-HETE but these mono-HETEs do not significantly contribute to the biological activity.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001202:Ascitic Fluid / Q000166:cytology; D000001:Calcimycin / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D007975:Leukotriene B4; D008084:Lipoxygenase / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism; D051381:Rats
D000900:Anti-Bacterial Agents; D001095:Arachidonic Acids; D007975:Leukotriene B4; D000001:Calcimycin; D008084:Lipoxygenase