Repair and mutagenesis of herpes simplex virus in UV-irradiated monkey cells.
Mutagenic repair in mammalian cells was investigated by determining the mutagenesis of UV-irradiated or unirradiated herpes simplex virus in UV-irradiated CV-1 monkey kidney cells. These results were compared with the results for UV-enhanced virus reactivation (UVER) in the same experimental situation. High and low multiplicities of infection were used to determine the effects of multiplicity reactivation (MR). UVER and MR were readily demonstrable and were approximately equal in amount in an infectious center assay. For this study, a forward-mutation assay was developed to detect virus mutants resistant to iododeoxycytidine (ICdR), probably an indication of the mutant virus being defective at its thymidine kinase locus. ICpR-resistant mutants did not have a growth advantage over wild-type virus in irraidated or unirradiated cells. Thus, higher fractions of mutant virus indicated greater mutagenesis during virus repair and/or replication. The data showed that: (1) unirradiated virus was mutated in unirradiated cells, providing a background level of mutagenesis; (2) unirradiated virus was mutated about 40% more in irradiated cells, indicating that virus replication (DNA synthesis?) became mutagenic as a result of cell irradiation; (3) irradiated virus was mutated much more (about 6-fold) than unirradiated virus, even in unirradiated cells; (4) cell irradiation did not change the mutagenesis of irradiated virus except at high multiplicity of infection. High multiplicity of infection did not lead to higher mutagenesis in unirradiated cells. Thus the data did not demonstrate UVER or MR alone to be either error-free or error-prone. When the two processes were present simultaneously, they were mutagenic.
D000818:Animals; D002460:Cell Line; D004260:DNA Repair / Q000528:radiation effects; D004279:DNA, Viral / Q000528:radiation effects*; D004352:Drug Resistance, Microbial; D005814:Genes, Viral; D000882:Haplorhini; D007065:Idoxuridine / Q000494:pharmacology; D007668:Kidney; D010641:Phenotype; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000528:radiation effects
D004279:DNA, Viral; D013937:Thymidine Kinase; D007065:Idoxuridine