Inhibition of 2',3'-cyclic nucleotide 3'-phosphodiesterase activity from bovine, human and guinea pig brain.
The purified oligodendrocyte enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) obtained from bovine, human and guinea pig brain was inhibited by theophylline, caffeine, cupric chloride and organomercurials using a spectrophotometric assay and 1 mM 2',3'- cyclic cytidylate as the substrate. The observed inhibition by theophylline, caffeine and organomercurials was reversible.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000037:antagonists & inhibitors*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002110:Caffeine / Q000494:pharmacology; D002417:Cattle; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D009836:Oligodendroglia / Q000201:enzymology; D009941:Organomercury Compounds / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors; D010727:Phosphoric Diester Hydrolases; D013806:Theophylline / Q000494:pharmacology
D009941:Organomercury Compounds; D010726:Phosphodiesterase Inhibitors; D002110:Caffeine; D013806:Theophylline; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases