Poly(A) polymerase activity in human rotavirus.
A poly(A) polymerase enzymic activity was found in partially purified preparations of a human rotavirus. The activity was demonstrated using conditions similar to those utilized for the detection of the poly(A) polymerase previously described in reovirus (incubation at 43 degrees C in 70 mM-tris buffer pH 7.5 containing 12 mM-Mn2+). The enzymic activity was associated only with complete, double-shelled particles. Characterization of the poly(A)-containing product of the in vitro reaction by gel filtration on Sephadex G-100, followed by chromatography on DEAE-cellulose in the presence of 7 M-urea, showed that it is composed of oligonucleotides of a chain length similar to, or slightly larger than, those observed in reovirus.
D000255:Adenosine Triphosphate / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006801:Humans; D009713:Nucleotidyltransferases / Q000378:metabolism*; D009841:Oligonucleotides / Q000032:analysis; D011061:Poly A / Q000096:biosynthesis; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D012087:Reoviridae / Q000201:enzymology*; D012401:Rotavirus / Q000201:enzymology*; D014771:Virion / Q000201:enzymology
D009841:Oligonucleotides; D011061:Poly A; D000255:Adenosine Triphosphate; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase; D012321:DNA-Directed RNA Polymerases