[Chemical composition of calcium channels of characean algal cells].
The present study was performed to determine the chemical composition and molecular weight of channel-forming complex by fractioning the cell homogenate supernatant by different means (chromatography, gel-electrophoresis, using dissociating and denaturating agents). Availability of the channel-forming complexes in individual fractions is judged from reconstruction of channels in BLM. The Ca-channels reconstructed in BLM are formed by molecules of a protein nature (presumably, peptides). Their molecular weight is no more than 20000 (presumably, 5000). A high stability of spacial organization of channel forming molecules was observed. The channels--subunits aggregate without any change in properties of the selective filter. The thermodynamically preferred agagregates have conductivity of 200 pmho in 0.1 M KCl and seem to consist of 80 channels--subunits which are switched off and on all together.
D002118:Calcium / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D000460:Chlorophyta / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels / Q000032:analysis*; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000032:analysis*; D008954:Models, Biological; D008970:Molecular Weight
D007473:Ion Channels; D008051:Lipid Bilayers; D008565:Membrane Proteins; D002118:Calcium
O khimicheskom sostave kal'tsievykh kanalov kletok kharovoĭ vodorosli.