Terminal strand-switching of E. coli RNA polymerase transcribing a truncated DNA fragment.
When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of Escherichia coli, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D008854:Microscopy, Electron; D008970:Molecular Weight; D009876:Operon; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes