Use of transposons in cloning poorly selectable genes of Escherichia coli: cloning of uvrA and adjacent genes.
A transposon was introduced close to a poorly selectable gene. This gene could be cloned by using selection for the antibiotic resistance marker of the transposon.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods*; D004260:DNA Repair; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010957:Plasmids
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements