DNA synthesis and multinucleation of mouse cells infected with SV40 in the presence of cytochalasin B.
DNA synthesis and nuclear division of mouse cells, BALB/3T3, infected with SV40 were studied and were compared with those of SV40-transformed mouse cells, mKS-A TU-7. Both SV40-infected and SV40-transformed cells behaved similarly in the presence of cytochalasin B and differently from normal noninfected cells, BALB/3T3. The chemical inhibited cytokinesis of all the cell groups but the nuclear division was inhibited only in the case of non-infected BALB/3T3. With SV40-infected BALB/3T3, multinucleation occurred with the increase of input m.o.i. by SV40. SV40-infected BALB/3T3 could enter the second S phase after release from double thymidine block in the presence of cytochalasin B, while BALB/3T3 could not enter the second S phase. In bi- or multinucleated cells of SV40-infected BALB/3T3, asynchrony of DNA synthesis among nuclei in a cell was evident, as was the case with mKS-A TU-7.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003571:Cytochalasin B / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D007399:Interphase; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D013539:Simian virus 40 / Q000254:growth & development*
D003571:Cytochalasin B; D004247:DNA