Effect of polyamines on purified poly(ADP-ribose) synthetase from rat liver nuclei.
Using the purified enzyme system, we investigated the spermine-induced stimulation of poly(ADP-ribose) formation. In the presence of 2 microgram DNA and 10 mM Mg2+ in a reaction mixture of 0.2 ml, the optimal concentrations of spermine, spermidine and putrescine were 10, 12.5 and 35 mM, respectively. With the omission of Mg2+, there was an increase in optimal concentrations of polyamines without changes in the rate of poly(ADP-ribose) formation. The concentrations of spermine required for maximum synthesis of poly(ADP-ribose) were increased in proportion to the increase in the amount of DNA. Kinetic analysis of poly(ADP-ribose) formation showed a decrease in Km values for NAD with the addition of spermine. The possible relationship of polyions with regard to DNA, spermine and histone in the purified enzyme system was discussed.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011073:Polyamines / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D051381:Rats; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D006657:Histones; D011073:Polyamines; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D013096:Spermine; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase; D008274:Magnesium; D013095:Spermidine; D011700:Putrescine