Evaluation of the drug-induced morphological differentiation of rat glioma cells (C-6) from the aspects of S-100 protein level and con A binding pattern.
The intracellular content of the nervous system specific protein S-100 began to increase with 4 days latency following the morphological differentiation of cultured rat glioma cells (C-6) with 1 mM dibutyryl cyclic AMP (dbcAMP), rising to approximately 10-fold over the control level at 15 days after the treatment. The concanavalin A (Con A) binding pattern on the external cell surface of C-6 cells exposed to dbcAMP appeared as a smooth layer of 40-60 nm thickness whereas that of control cells was irregularly thick and patchy. The correlation between morphological and biochemical changes of C-6 cells after dbcAMP treatment is discussed in relation to the mechanism controlling the differentiation of glioma cells.
D000818:Animals; D001665:Binding Sites; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D003208:Concanavalin A / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005910:Glioma / Q000032:analysis / Q000473:pathology* / Q000648:ultrastructure; D007700:Kinetics; D009374:Neoplasms, Experimental / Q000032:analysis / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D009418:S100 Proteins / Q000032:analysis*
D004273:DNA, Neoplasm; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D003208:Concanavalin A; D003994:Bucladesine