title: Progesterone production by dispersed monkey (Macaca mulatta) luteal cells after exposure to trypsin.
abstract: In an attempt to justify use of trypsin to achieve more thorough dispersion of luteal cell clumps in vitro, progesterone (P) production by collagenase dispersed monkey luteal cells from the mid-luteal phase corpus luteum (CL) was examined in vitro either after 10 min, or continuous (3h) exposure to trypsin (TR). In the first experiment, cells were pre-incubated in TR, then incubated at 37 degrees C for 3h with human chorionic gonadotropin (hCG) after the addition of soybean-trypsin inhibitor (STI). Pre-incubation of luteal cells with TR had no effect on the level of P production under basal conditions. Cells that were preincubated with TR responded to hCG stimulation with increased progesterone secretion (P less than 0.01) in a fashion similar to untreated cells. P production in response to hCG was independent of TR concentration over the range of 0.05% to 0.2% during the pre-incubation period. However, continuous exposure (3h) of cells to TR significantly depressed (P less than 0.01) basal P secretion and inhibited the response to hCG. We conclude that TR had no effect on the biopotency of hCG per se, but probably the over-exposure to TR had an adverse effect on the LH/hCG receptors. Addition of STI after a 10 min pre-incubation with TR, prevented these deliterious effects, thereby permitting the use of TR to improve the completeness of luteal cell dissociation.
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D000882:Haplorhini; D066298:In Vitro Techniques; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism; D008253:Macaca mulatta; D011374:Progesterone / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D006063:Chorionic Gonadotropin; D011956:Receptors, Cell Surface; D011374:Progesterone; D007986:Luteinizing Hormone; D014357:Trypsin