[Synthesis of cyclic AMP from renal cortex in vitro (author's transl)].
The parathyroid-hormone sensitive adenylate-cyclase from renal rat cortex has been studied. Mg2+ acts as stimulator at a specific locus increasing the apparent Vmax slightly from 50 to 60 nmol of cAMP per gram of protein if the Mg2+ concentration increases from 3.25 to 16 mM, and decreases the apparent KM from 2 to 0.4 mM. Fluoride decreases the apparent KM from 5 mM in NaF concentrations of 0 to 2 mM in NaF concentrations 2 mM. Magnesium ion also decreases the apparent Ka for NaF.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000494:Allosteric Regulation; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology
D012969:Sodium Fluoride; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D008274:Magnesium
Síntesis de AMP cíclico por la corteza renal in vitro.