Aging of the erythrocyte. IV. Spin-label studies of membrane lipids, proteins and permeability.
Spin-label studies demonstrated age-related alterations of the erythrocyte membrane concerning both lipid and protein components. Decrease in fluidity of membrane lipids correlated with decreased membrane permeability to a hydrophobic spin label TEMPO, permeability to a more hydrophilic TEMPOL being less affected. The rigidification of membrane lipids was much more pronounced in whole membranes than in liposomes composed of membrane lipids, suggesting changes in lipid-protein interactions as an important factor in the decrease of lipid fluidity in aged red cells. ESR spectra of membrane-bound maleimide spin label evidenced alterations in the state of membrane proteins during cell aging in vivo.
D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D008563:Membrane Lipids / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D008563:Membrane Lipids; D008565:Membrane Proteins; D013113:Spin Labels; C001803:tempol; C003959:TEMPO