Modulation of CALLA and HLA-DR on a non T non B leukemic cell line by lipid treatment.
The relationship between membrane lipid fluidity and expression of HLA-DR and cALL (CALLA) antigens was studied in a human non T non B acute lymphoblastic leukemia cell line (Reh). The membrane fluidity was modulated by treatment with cholesteryl hemisuccinate or phospholipids (e.g. egg lecithin) and monitored by fluorescence polarization. HLA-DR and CALLA expression was measured in an indirect immunofluorescence test with a Fluorescence Activated Cell Sorter (FACS 440), on 24, 48, 72 and 96 hour-cultured cells. Significant antigenic modulation was obtained with cholesteryl hemisuccinate treatment on 48 hour-cells where a slight increase in HLA-DR and a marked decrease in CALLA were observed. In contrast no antigenic modification was observed on lecithin-treated cells.
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D002784:Cholesterol / Q000494:pharmacology*; D005454:Fluorescence Polarization; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007945:Leukemia, Lymphoid; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000502:physiology*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D002784:Cholesterol