title: Influence of gonadotrophins on cAMP formation in theca cells isolated from pre-ovulatory rat follicles.
abstract: Pre-ovulatory follicles were obtained from immature PMSG treated rats at various times on the day of pro-oestrus. From these follicles the theca capsules were mechanically isolated and subsequently incubated in the presence or absence of LH and/or FSH. The accumulation of cyclic adenosine 3'5'-monophosphate (cAMP) in tissue plus medium was determined. In certain experiments the rats were injected with LH, FSH or saline 2 h prior to sacrifice. When the theca cells were isolated from rats sacrificed before the endogenous gonadotrophin surge, FSH added in vitro did not significantly stimulate cAMP formation, while LH had a small but significant stimulatory effect. When isolated 2-5 h after the endogenous gonadotrophin surge the theca cells responded with a marked increase in cAMP formation when exposed to LH in vitro, whereas FSH in vitro still had no stimulatory effect. Theca cells, isolated before the endogenous gonadotrophin surge, from rats pre-treated with a single ip injection of FSH 2 h prior to sacrifice, responded to a subsequent in vitro exposure to LH with a marked increase in cAMP levels. The results indicate that the FSH component of the endogenous gonadotrophin surge increases the sensitivity of the theca cells to LH - i.e. in vivo exposure to FSH seems to be essential for the development of responsiveness of the cAMP system to LH in the theca cells from pre-ovulatory rat follicles.
mesh_terms: D000818:Animals; D002469:Cell Separation; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011247:Pregnancy; D011359:Proestrus; D051381:Rats; D013799:Theca Cells / Q000378:metabolism*; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP