Conformational changes of yeast tRNAPhe upon interaction with intercalators probed by nuclease digestion.
The conformational changes of yeast-tRNAPhe induced by various intercalators have been studied using limited digestions with nucleases S1 and T1. The results show an increased sensitivity to T1 in the D-loop, suggesting a weakening of the D-loop-T-loop interaction. Furthermore, the results are best explained by a non-intercalative binding of the eyes, probably in the D-loop-T-loop cavity.
D000585:Aminacrine / Q000494:pharmacology; D004720:Endonucleases; D004996:Ethidium / Q000494:pharmacology; D007364:Intercalating Agents / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D006163:Ribonuclease T1; D015719:Single-Strand Specific DNA and RNA Endonucleases
D007364:Intercalating Agents; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D000585:Aminacrine; D004720:Endonucleases; D006163:Ribonuclease T1; D015719:Single-Strand Specific DNA and RNA Endonucleases; D004996:Ethidium