Appearance of beta-lactamase activity in animal cells upon liposome-mediated gene transfer.
A restriction fragment, 875 bp, which encodes for a beta-lactamase activity, was isolated from the Escherichia coli plasmid pBR322 DNA and entrapped in liposomes. The incubation of the DNA-liposomes with avian, murine, and human cultured cells results in the uptake of the DNA with the efficiency of around 2000 molecules per cell. Extracts of the recipient cells show a beta-lactamase activity as demonstrated by spectroscopic and microbiological methods. These results indicate the expression of a prokaryotic gene in eukaryotic cells.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D008081:Liposomes; D010957:Plasmids; D013053:Spectrophotometry; D014170:Transformation, Genetic; D001618:beta-Lactamases / Q000378:metabolism*
D008081:Liposomes; D004262:DNA Restriction Enzymes; D001618:beta-Lactamases