Involvement of mu-receptors in the opioid-induced generation of bursting discharges in hippocampal pyramidal cells.
Cultured hippocampal pyramidal cells responded to field stimulation with a short latency excitation followed by a long-lasting inhibition. This sequence was transformed into a bursting response by bath application of 10(-8) M FK 33-824, 10(-6) M (D-Ala)2(D-Leu)5-enkephalin and 10(-5) M bremazocine. Bremazocine and ethylketocyclazocine stereospecifically blocked the effects of FK 33-824. The results indicate that the excitatory responses were predominantly mediated by mu-receptors.
D000818:Animals; D001575:Benzomorphans / Q000031:analogs & derivatives / Q000494:pharmacology*; D046508:Culture Techniques; D003496:Cyclazocine / Q000031:analogs & derivatives / Q000494:pharmacology; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D004723:Endorphins / Q000494:pharmacology*; D016308:Enkephalin, Leucine-2-Alanine; D004745:Enkephalins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016314:Ethylketocyclazocine; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009019:Morphinans / Q000494:pharmacology*; D051381:Rats; D011957:Receptors, Opioid / Q000502:physiology*; D017450:Receptors, Opioid, mu
D001575:Benzomorphans; D004723:Endorphins; D004745:Enkephalins; D009019:Morphinans; D011957:Receptors, Opioid; D017450:Receptors, Opioid, mu; D016314:Ethylketocyclazocine; D016308:Enkephalin, Leucine-2-Alanine; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin; C027248:bremazocine; D003496:Cyclazocine