Characteristics of arterial plasma membrane in renovascular hypertension in rats.
Protein content, enzymatic activites and Ca2+ accumulation capacities were studied in plasma membrane fractions isolated from mesenteric arteries of rats made hypertensive by renal artery stenosis with and without contralateral nephrectomy, i.e., one-kidney, one clip (1-KHR) and two-kidney, one clip (2-KHR) hypertension, respectively. Both types of renovascular hypertension showed similar vascular plasma membrane abnormalities which included increased total protein contents, enhanced alkaline phosphatase activities and reduced ATP-dependent Ca2+ accumulation compared to control values. The altered alkaline phosphatase activity and ATP-dependent Ca2+ accumulation appeared to be associated with blood pressure elevation in both types of hypertension and may be related to the elevation of blood pressure insensitive to captopril (SQ 14,225) in 1-KHR and 2-KHR. These results are consistent with the current concept of biochemical abnormalities of arterial smooth muscle in the development ostem.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology; D002462:Cell Membrane / Q000503:physiopathology; D006977:Hypertension, Renal / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D008638:Mesenteric Arteries / Q000503:physiopathology*; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats; D012078:Renal Artery Obstruction / Q000150:complications
D000001:Calcimycin; D002216:Captopril; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D002118:Calcium