Water radiolysis products and nucleotide damage in gamma-irradiated DNA.
The radiation chemistry of nucleotide damage introduction into DNA gamma-irradiated in dilute aqueous solution has been studied with a damage-specific DNA binding protein. Irradiation in air, N2 or N2O in the presence and absence of free radical scavengers revealed that protein-recognizable lesions were introduced both by the hydrated electron and by the combination of the hydroxyl radical and superoxide anion radical, but not by the hydroxyl radical alone. In addition, nucleotide damage was introduced into DNA by an enzymatic superoxide-generating system.
D002352:Carrier Proteins / Q000494:pharmacology; D004247:DNA / Q000494:pharmacology; D004260:DNA Repair; D004279:DNA, Viral / Q000528:radiation effects*; D004268:DNA-Binding Proteins; D005609:Free Radicals; D005720:Gamma Rays; D006801:Humans; D006878:Hydroxides; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D009247:NADH, NADPH Oxidoreductases / Q000494:pharmacology; D019255:NADPH Oxidases; D009711:Nucleotides / Q000528:radiation effects*; D013481:Superoxides / Q000494:pharmacology; D013604:T-Phages; D014867:Water / Q000528:radiation effects*
D002352:Carrier Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D005609:Free Radicals; D006878:Hydroxides; D009711:Nucleotides; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; D004247:DNA; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; C022709:superoxide-forming enzyme