The effect of glucagon and prostaglandin E1 on cyclic AMP levels in liver of heat exposed hamsters.
Cyclic AMP levels in liver slices of hamsters exposed to 35 degrees C for 21 days and controls maintained at 22 degrees C was found to be similar in basal conditions. Glucagon (10 microgram/ml) caused 3.5 times elevation of cyclic AMP levels in control hamsters and 9 times elevation in 35 degrees C exposed hamsters, thus a difference of 150% of the nucleotide concentration was found between the two experimental groups. When 10(-2)M theophylline was added, the cyclic AMP levels were 80% higher in 35 degrees C exposed hamsters both in the presence and absence of 10 microgram/ml glucagon. The difference between controls and heat exposed animals was found to be the same when various concentrations of both glucagon or prostaglandin E1 were added to the liver slices. Adenylate cyclase activity was similar in both experimental groups, while low Km phosphodiesterase was significantly less active in the liver of 35 degrees C exposed animals when compared to the controls.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism*; D005934:Glucagon / Q000494:pharmacology*; D006358:Hot Temperature; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology
D011458:Prostaglandins E; D005934:Glucagon; D013806:Theophylline; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases