The in situ determination of melting-solidification points of lipid inclusions in fixed cultured cells.
Melting-solidification points of microscopic lipid inclusions in fetal human kidney cultures were determined visually by gradual heating of formol-fixed cell monolayers while immersed in dilute aqueous solutions of Nile blue A. Intracellular lipid inclusions stained red when their melting point was reached and reverted to blue color at the solidification temperature. The melting-solidification points of intracellular lipid inclusions in kidney cells were determined to fall within 19-25, 40-50, and 60-65 degrees C range.
D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005333:Fetus; D006358:Hot Temperature; D006801:Humans; D002479:Inclusion Bodies / Q000032:analysis*; D007668:Kidney; D050356:Lipid Metabolism