Catecholamine-induced stimulation of adenylate cyclase in parallel with a change in tubulin assembly in Ehrlich ascites tumor cells.
In vitro incubation of Ehrlich ascites tumor cells in the presence of norepinephrine induced desensitization of adenylate cyclase to the later norepinephrine stimulation. Such a desensitization was not accompanied by a decrease in the number of receptor sites. Formation of actin filaments from actin monomers was not changed in the desensitized cells, whereas polymerization of tubulin was significantly increased. The increase in the polymerizaton was dependent on the concentration of norepinephrine.
D000199:Actins / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002478:Cells, Cultured; D009638:Norepinephrine / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D014404:Tubulin / Q000378:metabolism*
D000199:Actins; D011943:Receptors, Adrenergic, beta; D014404:Tubulin; D000262:Adenylyl Cyclases; D009638:Norepinephrine