Physical properties of the complementary T4 RNA.
The complementary transcribed T4 RNA after self-annealing and RNAase treatment was isolated by gel chromatography and then used for further studies. From salt-dependent RNAase resistance and melting studies it is evident that this RNA represents a genuine double-stranded structure. The base content of the isolated double-stranded RNA was found to be the same as total T4 mRNA. Sucrose gradient analysis and hydroxyapatite chromatography of T4 RNA, annealed early and late RNA, and of the isolated double-stranded RNA, gave results indicating that the complementary RNA is part of a RNA molecule and further that the size of the complementary regions are independent of the RNA molecules. Partial digestion of pulse-labelled late RNA with phosphodiesterase I prior to annealing with unlabelled early RNA, showed that the complementary regions on the mRNA are not located to the 5'- or 3'-end but randomly distributed along the T4 RNA molecules.
D004926:Escherichia coli / Q000032:analysis; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D010727:Phosphoric Diester Hydrolases; D012367:RNA, Viral / Q000302:isolation & purification; D012260:Ribonucleases; D013604:T-Phages / Q000032:analysis*
D012367:RNA, Viral; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases