title: Characterization of yeast iso-1-cytochrome c mRNA.
abstract: The iso-1-cytochrome c mRNA has been identified by hybridization of a 32P probe prepared from a plasmid containing the iso-1-cytochrome c gene to RNA size-fractionated on agarose gels and transferred to paper. A hybridization band was visible with RNA prepared from wild type cells, but not with RNA prepared from an iso-1-cytochrome c deletion mutant. RNA prepared from cells containing a nonsense mutation in the iso-1-cytochrome c gene showed reduced levels of hybridization. The RNA that hybridized to the probe was 700 +/- 50 nucleotides in length and was polyadenylated. The cellular levels of this RNA were repressed by glucose, and this repression was achieved within 5 min after glucose addition to a derepressed culture. No precursors of this RNA were detected in wild type cells or in an RNA1 mutant, temperature-sensitive for RNA metabolism. The length of the 3' noncoding region of this RNA was determined to be 200 +/- 25 nucleotides (excluding the poly(A) tail) and the 5' noncoding region was estimated to be about 120 nucleotides in length.
mesh_terms: D001483:Base Sequence; D003574:Cytochrome c Group / Q000031:analogs & derivatives* / Q000096:biosynthesis; D045304:Cytochromes c; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D005947:Glucose / Q000378:metabolism; D008970:Molecular Weight; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic
chemical_list: C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D004274:DNA, Recombinant; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D011061:Poly A; D045304:Cytochromes c; D005947:Glucose