[Comparative study of normal and psoriatic skin using a micromethod for detection of enzymatic activity].
We used a semiquantitative micromethod for detection of enzymatic activity (APIZYM) which allows a fast and simultaneous study of 67 hydrolase and dehydrogenase activities on dermo-epidermic skin samples. This wide investigated field led to the determination of the enzymatic profile (called zymogram) of the samples under study. This method was applied to normal skin and to psoriatic and uninvolved psoriatic skin; our results exhibited the good reproductibility of the method and the homogeneity of the studied samples. The enzymatic activities of the psoriatic skin are, in mean, twice those of normal skin. The uninvolved psoriatic skin showed reactions close to those of the normal skin. These observed variations of those different activities are perfectly similar to literature datas. The APIZYM method, thanks to its easy utilization and its fiability seems extremely interesting in the study of zymogram of cutaneous samples. The use of APIZYM in observations on naevus and malignant melanoma is to be exposed in a following publication.
D000328:Adult; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010088:Oxidoreductases / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011565:Psoriasis / Q000201:enzymology*; D012867:Skin / Q000201:enzymology*
D010088:Oxidoreductases; D006867:Hydrolases; D010744:Phosphoric Monoester Hydrolases; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases
Etude comparée de la peau saine et psoriasique par une microméthode de détection d'activités enzymatiques.