Cell substrate and risk in killed poliomyelitis vaccine.
By using 3H-T labeled HeLa cell DNA it was possible to evaluate the quantity and the state of substrate DNA present in crude and purified killed poliovaccine. The results showed that 1.7 x 10(5) pg/ml of DNA is present in crude vaccine while in purified vaccine this product can not be detected (the limit of the detection method was 1.1 x 10(2) pg/ml). The theoretical problems of the risk of the formation of poliovirus pseudotypes and the potential contamination of the vaccine with a type C retrovirus are examined.
D002460:Cell Line; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D011054:Poliovirus Vaccine, Inactivated / Q000302:isolation & purification*; D012190:Retroviridae / Q000302:isolation & purification; D012306:Risk
D004273:DNA, Neoplasm; D004279:DNA, Viral; D011054:Poliovirus Vaccine, Inactivated