Cyclic AMP binding proteins in the rat thyroid cytosol. Effects of suppression and stimulation of thyroid activity.
Suppression of thyroid activity by treatment of rats with thyroxine in vivo significantly decreases the relative concentration of protein kinase regulatory subunits in the thyroid cytosol. Stimulation by thyroid-stimulating hormone (TSH) in rats previously treated by thyroxine for 5 days significantly increases the relative concentration of regulatory subunits. Two species of regulatory subunits of protein kinases were separated by ion exchange chromatography. The observed modifications after thyroxine and TSH treatment affect both species of regulatory subunits to the same extent, whereas chronic stimulation by propylthiouracil for 30 days preferentially increases the type II regulatory subunit.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D000242:Cyclic AMP / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011441:Propylthiouracil / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology; D013974:Thyroxine / Q000494:pharmacology
D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D011441:Propylthiouracil; D013972:Thyrotropin; D000242:Cyclic AMP; D011494:Protein Kinases; D013974:Thyroxine