[Induction of transposon Tn1 translocation in UV-irradiated Escherichia coli cells].
Ampicillin transposon Tn1 translocates from plasmid RP4 into E. coli chromosome with a frequency of about 3.10(-4) per cell. Irradiation of bacteria with UV-light increases the frequency of translocation essentially. Mutation in lexA gene controlling the expression of cell UV-inducible functions blocks the induction of transposon translocation. Protein synthesis inhibitor--chloramphenicol and transcription inhibitor--rifampicin decrease the effect of UV-light on transposition process. A possible mechanism of inducible transposition is discussed.
D000667:Ampicillin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D009154:Mutation; D010957:Plasmids; D012293:Rifampin / Q000494:pharmacology; D014178:Translocation, Genetic; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D002701:Chloramphenicol; D000667:Ampicillin; D012293:Rifampin
Induktsiia translokatsii transpozona Tn1 v obluchennykh UF-svetom kletkakh Escherichia coli.