The effect of dimethyl sulfoxide on gray matter injury in experimental spinal cord trauma.
The possible effect of dimethyl sulfoxide upon the development of lesions in the gray matter after experimental spinal cord trauma has been investigated with the use of cytochrome oxidase assay and quantitative histologic measurement of total liquefaction necrosis. Observations were made in 17 unconditioned dogs receiving an impact trauma of 400 gm cm force. Experimental animals were given 2.5 gm/kg of dimethyl sulfoxide in 40% solution intravenously one hour prior to trauma, and control animals received a similar volume of saline. No reduction could be found in the degree of loss of cytochrome oxidase at one hour after trauma, nor in the extent of acute necrosis. A slight but non-significant increase in the amount of hemorrhage was noted in gray matter at the trauma site following treatment with dimethyl sulfoxide. The agent resulted in an increase in cytochrome oxidase activities in nontraumatized control gray matter.
D000818:Animals; D001929:Brain Edema / Q000188:drug therapy; D046508:Culture Techniques; D004121:Dimethyl Sulfoxide / Q000494:pharmacology* / Q000627:therapeutic use; D004285:Dogs; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D008297:Male; D013116:Spinal Cord / Q000187:drug effects* / Q000201:enzymology; D013119:Spinal Cord Injuries / Q000201:enzymology / Q000517:prevention & control*
D003576:Electron Transport Complex IV; D004121:Dimethyl Sulfoxide