Production of human IgM anti-D in tissue culture by EB-virus-transformed lymphocytes.
Lymphocytes obtained from two donors who were producing anti-D were transformed with Epstein-Barr virus and grown in tissue culture. The lymphoblasts from both donors produced anti-D antibody, which agglutinated Rh-positive cells in saline. Both antibodies belonged to the IgM class; the concentration of anti-D in the culture fluid from one of the donors was approximately 250--500 ng/ml and bound firmly to Rh-positive cells with a functional affinity constant  of approximately 1 x 10(9) M-1.
D000017:ABO Blood-Group System; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008214:Lymphocytes; D012204:Rh-Hr Blood-Group System; D012397:Rosette Formation
D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012204:Rh-Hr Blood-Group System