Ecto-5'-nucleotidase activity in lymphoblastoid cell lines derived from heterozygotes for congenital X-linked agammaglobulinemia.
Lymphoblastoid cell lines were established from female relatives of patients with congenital X-linked-agammaglobulinemia by Epstein-Barr virus transformation of their peripheral B lymphocytes. Cell lines derived from presumed carriers were characterized by low ecto-5'-nucleotidase activity and a reduced percentage of surface immunoglobulin-bearing cells. Measurement of ecto-5'-nucleotidase activity in newly established lymphoblastoid cell lines may provide a means for the identification of heterozygotes for congenital X-linked agammaglobulinemia.
D015720:5'-Nucleotidase; D000361:Agammaglobulinemia / Q000151:congenital*; D002460:Cell Line; D005260:Female; D006579:Heterozygote; D006801:Humans; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D010375:Pedigree; D011947:Receptors, Antigen, B-Cell; D012730:Sex Chromosomes / Q000276:immunology*; D014960:X Chromosome / Q000276:immunology*
D011947:Receptors, Antigen, B-Cell; D009708:Nucleotidases; D015720:5'-Nucleotidase