Transformation of rat fibroblast cells with early mutants of polyoma (tsa) and simian virus 40 (tsA30): occurrence of either A or N transformants depends on the multiplicity of infection.
Infection of rat fibroblasts with early mutants of polyoma virus (tsa) or simian virus 40 (tsA30) leads to the establishment of either temperature-independent A transformants or N transformants temperature-sensitive for the expression of the transformed phenotype. The choice between the A- and N-transformed states is not only dependent, as we reported previously (Rassoulzadegan et al., j. Virol., 28:421-426, 1978), on the growth conditions after infection, but is also a function of the multiplicity of infection (MOI); high MOI led to the predominant occurrence of A derivatives, and lower MOI led to that of N transformants.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D005347:Fibroblasts; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*; D013696:Temperature