Subcellular localization of chicken kidney aryl acylamidase activity.
The intracellular localization of aryl acylamidase (aryl-acylamide amidohydrolase, EC 3.5.1.13) in chicken kidney was investigated. By separation on density gradients of the silica sol Ludox AM, the enzyme was localized in the mitochondrial fraction. This mitochondrial fraction was shown to be substantially free of lysosomal contamination. Subfractionation of the purified mitochondria indicates that the enzyme is located on the outer membrane, can be solubilized, and may be a suitable marker enzyme for kidney mitochondria.
D000581:Amidohydrolases / Q000302:isolation & purification / Q000378:metabolism*; D000813:Anilides / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002500:Centrifugation, Isopycnic; D002645:Chickens; D007668:Kidney / Q000201:enzymology*; D012822:Silicon Dioxide; D012995:Solubility; D013010:Sonication; D013347:Subcellular Fractions / Q000201:enzymology; D013367:Submitochondrial Particles / Q000201:enzymology; D014018:Tissue Distribution
D000813:Anilides; C024922:Ludox AM; D012822:Silicon Dioxide; D000581:Amidohydrolases; C019732:aryl acylamidase