title: Deoxyribonucleases in herpes simplex virus type 1 and 2 infected primary rabbit kidney cells.
abstract: In primary rabbit kidney cells infected with herpes simplex virus four different neutral deoxyribonuclease activities can be detected by means of the deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. The method is suitable to follow independently the change in each activity of the different enzymes using only about 5 X 10(5) cells for each assay during the time-course of infection. Under these conditions one enzyme activity is constant, two disappear while the activity of a fourth one present only in infected cells, increases.
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003851:Deoxyribonucleases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D011817:Rabbits; D018139:Simplexvirus / Q000201:enzymology*
chemical_list: D003851:Deoxyribonucleases