Dissociation of the DNAse-I . actin complex by formamide.
Rabbit skeletal muscle actin labeled with 125 iodine by an enzymic method is shown to be capable of polymerization and to bind to matrix-bound pancreatic DNAse I like unlabeled G-actin. It was used to demonstrate that actin can be released from DNAse-I-agarose by 35--40% formamide. Actin which was only shortly exposed to this solvent was able to bind again to DNAse I and to form filaments indicating that it has been recovered functionally intact from the affinity matrix.
D000199:Actins / Q000302:isolation & purification; D000818:Animals; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases; D004720:Endonucleases; D005559:Formamides; D007457:Iodine Radioisotopes; D007700:Kinetics; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009132:Muscles / Q000032:analysis; D011485:Protein Binding; D011817:Rabbits; D012685:Sepharose
D000199:Actins; D005559:Formamides; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D012685:Sepharose; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I