Fluoride, pyrophosphate, and base release from 2'-deoxy-2'-fluoronucleoside 5'-diphosphates by ribonucleoside-diphosphate reductase.
Ribonucleoside-diphsophate reductase from Escherichia coli catalyzes release of fluoride, inorganic pyrophosphate, and base from 2'-deoxy-2'-fluoronucleoside diphosphates. This reaction is accompanied by inactivation of the enzyme and an increase in absorbance at 314 nm of the inactivated protein. 2'-Deoxy-2'-fluoroadenosine 5'-diphosphate requires two turnovers per inactivation, whereas 2'-deoxy-2'-fluorocytidine 5'-diphosphate requires 100 turnovers per inactivation.
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives*; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D011756:Diphosphates / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology*; D005459:Fluorides / Q000032:analysis; D007700:Kinetics; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism*; D012264:Ribonucleotide Reductases / Q000378:metabolism*
C025354:2'deoxy-2'-fluorocytidine 5'-diphosphate; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D011756:Diphosphates; D000244:Adenosine Diphosphate; C025353:2'-deoxy-2'-fluoroadenosine 5'-diphosphate; D012264:Ribonucleotide Reductases; D012262:Ribonucleoside Diphosphate Reductase; D005459:Fluorides