title: Metabolic requirements for rabbit polymorphonuclear leucocyte lipoxygenase activity.
abstract: Lipoxygenase product formation by rabbit PMNs on exposure to the divalent ionophore A23187 was dependent upon pH, metabolic energy and substrate availability. Formation of 5-HETE and 5,12-diHETE (the major arachidonate oxygenation products) was inhibited by calcium depletion, by the metabolic inhibitors iodoacetate and 2-deoxyglucose, and by the substrate inhibitor TYA. The reaction had a pH optimum of 7.0-7.5. Colchicine, cytochalasin B and isoprenaline had no significant effects on HETE formation.
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis; D000001:Calcimycin / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids; D007545:Isoproterenol / Q000494:pharmacology; D007975:Leukotriene B4; D008084:Lipoxygenase / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D011817:Rabbits; D013997:Time Factors
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D000001:Calcimycin; D003571:Cytochalasin B; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D008084:Lipoxygenase; D007545:Isoproterenol; D003078:Colchicine