Identification of the tetracycline resistance promoter and repressor in transposon Tn10.
The structural and regulatory functions encoding tetracycline resistance in transposon Tn10 lie within a 2,700-base pair region. Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, we located the promoter controlling the expression of tetracycline resistance. These various sequences conferred altered levels of tetracycline resistance. Plasmids containing deletions of a 695-base pair HincII fragment were constitutive and showed the loss of a 23,000-dalton tetracycline-inducible polypeptide, thus identifying the repressor and the location of its gene.
D001426:Bacterial Proteins / Q000235:genetics; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012097:Repressor Proteins; D013752:Tetracycline