Rapid isolation of functionally intact pancreatic islets from mice and rats by percollTM gradient centrifucation.
A discontinuous gradient of Percoll was used to effectively separate islets of Langerhans from collagenase digested pancreas from mice and rats. Percoll isolated islets and islets isolated by direct hand-picking from the pancreatic digest were maintained in tissue culture. Percoll isolation of islets did not affect glucose-stimulated insulin release from islets cultured for one week nor the ability of islets from newborn rats to incorporate 3H-thymidine into DNA. Centrifugation in Percoll is an efficient method to isolate functionally intact pancreatic islets.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000379:methods; D003102:Colloids; D004247:DNA / Q000096:biosynthesis; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D011205:Povidone; D051381:Rats; D012822:Silicon Dioxide; D013936:Thymidine / Q000378:metabolism
D003102:Colloids; D007328:Insulin; C016039:Percoll; D012822:Silicon Dioxide; D004247:DNA; D011205:Povidone; D013936:Thymidine