Conductimetric study of the binding of Mn2+ to bovine pancreatic deoxyribonuclease.
The binding of Mn2+ on bovine pancreatic deoxyribonuclease has been studied by a conductimetric method. At low ionic strengths, a high-affinity single binding site is demonstrated. The association constant value (K = 1.2 x 10(5) M-1 at pH 8) is high enough to conclude that, in standard experimental conditions for DNA hydrolysis, the reacting species is the DNAase-cation complex. Competitive binding studies in presence of Mg++ and Ca++ show that these cations do not bind on the Mn++ site.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology*; D011485:Protein Binding
D008345:Manganese; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I