Investigations concerning the cultivation of myxo- and paramyxoviruses on chorioallantoic membrane fragments. Note I. Data on the multiplication of several myxo- and paramyxoviruses.
Influenza viruses A(H1N1) and A(H3N2) and parainfluenza viruses (Sendai mumps) were cultivated in chorioallantoic membrane (CAM) fragments maintained in media with different formulae, with or without daily medium changes, in roller or stationary tubes. Inoculation was performed either directly on CAM fragments in Petri dishes or by dilution of the virus-containing material in the medium. Infectant titers obtained in CAM fragments were similar to those recorded in embryonated eggs at 48 hours post inoculation (p.i.) in the case of influenza virus A(H1N1) and at 72 hours p.i. in that of Sendai and influenza A(H3N2) viruses; at 96 hours p.i. all the three viruses had titers superior to those found in the egg.
D000818:Animals; D002642:Chick Embryo; D003470:Culture Media; D005321:Extraembryonic Membranes; D009975:Orthomyxoviridae / Q000254:growth & development*; D010252:Paramyxoviridae / Q000254:growth & development*; D018108:Respirovirus; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
D003470:Culture Media