Detection of serum antibody to herpes simplex virus type I by enzyme-linked immunosorbent assay (ELISA).
For setting up an Enzyme-Linked Immunosorbent Assay (ELISA) to detect antibodies to herpes simplex virus type 1 (HSV-1), the virus was propagated in Vero cells and partially purified by sonification and ultracentrifugation on 30% sucrose solution. DEAE ion-exchange column chromatography was used for purification of goat anti-human IgG serum. The anti-human IgG immune serum and alkaline phosphatase were conjugated by glutaraldehyde method. ELISA test was performed by reacting HSV-1 antigen coated in polystyrene tubes with serum specimens and enzyme-IgG conjugates. The color produced by enzyme-substrate reaction was measured on a spectrophotometer. The results obtained by the ELISA had a good agreement with those obtained by a standard neutralization procedure on 119 serum specimens tested for antibody to HSV-1.
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral