[Comparison between 2 density gradients for separation of CFU].
We separated 40 bone marrow samples by centrifugation on two density gradients, ficoll-metrizoate sodium (lymphoprep) and percoll. There is no difference between the two methods when expressing the colony forming cells (CFU-C) per 2.10(5) bone marrow cells plated. But the absolute number of recovered CFU-C is significantly greater with percoll than with ficoll metrizoate sodium.
D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D003114:Colony-Forming Units Assay; D005362:Ficoll; D006801:Humans; D007463:Iodobenzoates; D008794:Metrizoic Acid; D011134:Polysaccharides; D011205:Povidone; D012822:Silicon Dioxide
D007463:Iodobenzoates; D011134:Polysaccharides; D005362:Ficoll; C016039:Percoll; D012822:Silicon Dioxide; D008794:Metrizoic Acid; D011205:Povidone
Comparaison entre deux gradients de densité pour séparer les CFC.