Scanning and transmission electron microscopy of cloned rat astrocytoma cells treated with dibutyryl cyclic AMP in vitro.
Two cloned rat astrocytoma cell lines, 36 B-10 and 40 A-2, maintained in vitro were treated with 1 mM dibutyryl cyclic AMP. This treatment induced arborization of cellular processes and rounding-up of cell bodies in both cell lines and was associated with increased microvillous development in 40 A-2. There were not detectable concomitant changes in either (a) the quantity or organization of microtubules or 80-100 nm microfilaments, or (b) the intensity of glial fibrillary acidic protein indirect immunofluorescence staining.
D000818:Animals; D001254:Astrocytoma / Q000648:ultrastructure*; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D002999:Clone Cells / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D051381:Rats
D003994:Bucladesine