Characterization of avian myeloblastosis-associated virus DNA intermediates.
The major species of unintegrated linear viral DNA identified in chicken embryonic fibroblasts infected with either the avian myeloblastosis-associated viruses (MAV-1, MAV-2) or the standard avian myeloblastosis virus complex (AMV-S) has a mass of 5.3 X 10(6) daltons. An additional minor DNA component observed only in AMV-S-infected cells has a mass of 4.9 X 10(6) daltons. The unintegrated linear viral DNAs and integrated proviruses of MAV-1 and MAV-2 have been analyzed by digestion with the restriction endonucleases EcoRI and HindIII. MAV-2 lacks a HindIII site present in MAV-1. These fragments have been compared to those generated by EcoRI and HindIII digestion of linear viral DNAs of AMV-S. Restriction enzyme digestion of AMV-S viral DNA produced unique fragments not found with either MAV-1 or MAV-2 viral DNAs. The major viral component present in AMV-S stocks has the HindIII restriction pattern of MAV-1. Restriction enzyme analysis of the 5.3 X 10(6)-dalton unintegrated MAV viral DNAs and their integrated proviruses suggests that the DNAs have a direct terminal redundancy of approximately 0.3 megadaltons and integrate colinearly with respect to the unintegrated linear DNA.
D001354:Avian Leukosis Virus / Q000032:analysis*; D009189:Avian Myeloblastosis Virus / Q000032:analysis* / Q000235:genetics; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D012526:Satellite Viruses / Q000032:analysis* / Q000235:genetics
D004279:DNA, Viral; D004262:DNA Restriction Enzymes