The inactivation of rat adipocyte Mg2+-dependent phosphatidate phosphohydrolase by noradrenaline.
The inactivation of rat adipocyte Mg2+-dependent phosphatidate phosphohydrolase by noradrenaline [Cheng & Saggerson (1978) FEBS Lett. 87, 65--68; Cheng & Saggerson (1978) FEBS Lett. 93, 120--124] persists for at least 40 min in crude defatted homogenates kept at 0 degrees C or 20 degrees C, but is diminished at 37 degrees C. The effect of noradrenaline persists through the isolation of post-105000 g supernatants and is then stable in these preparations at 0 degrees C and 37 degrees C. Inclusion of albumin (10--20 mg/ml) in homogenization buffers abolishes the effect of noradrenaline. The effect of noradrenaline is not removed by dialysis of extracts or by raising the concentrations of Mg2+ or phosphatidate in assays.
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002498:Centrifugation; D008274:Magnesium / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology*; D010711:Phosphatidate Phosphatase / Q000037:antagonists & inhibitors*; D010712:Phosphatidic Acids / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*; D051381:Rats; D013696:Temperature
D000418:Albumins; D010712:Phosphatidic Acids; D010744:Phosphoric Monoester Hydrolases; D010711:Phosphatidate Phosphatase; D008274:Magnesium; D009638:Norepinephrine