TCGF production for cloning and growth of functional human T lymphocytes.
In an effort to increase the potency of T cell growth factor (TCGF), several variables were examined for their effects on the production of TCGF. The following manipulations enhanced the potency of TCGF: first, the removal of adherent cells and addition of indomethacin to the producing cultures; second, irradiation with 1000 rads of the cells used to produce TCGF; and, third, the addition of Epstein-Barr virus transformed lymphoblastoid (LCL) cells. It was also noted that the addition of irradiated feeder cells increased the efficiency of limiting dilution cloning.
D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects; D013601:T-Lymphocytes / Q000166:cytology*
D007376:Interleukin-2; D008222:Lymphokines; D007213:Indomethacin