Subcloning of the histone DNA sequences of phage lambda Sam 7 h 22 in plasmid pBR 322.
The histone DNA sequences of the Hind III cluster of the sea urchin Psammechinus miliaris which are carried by the chimeric phage DNA of lambda Sam 7 h 22 have been subcloned in plasmid p BR 322. Due to this procedure single segments of the cluster have been separated from each other and are available as gene specific hybridization probes.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005796:Genes; D006657:Histones / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012617:Sea Urchins / Q000235:genetics
D004274:DNA, Recombinant; D006657:Histones; D004262:DNA Restriction Enzymes