Characterization and comparison of two plasma membrane reference enzymes with those of other organelles during mammalian embryogenesis.
1. Optimal assay conditions for two plasma membrane reference enzymes, alkaline phosphodiesterase 1 and 5'-nucleotidase, were determined in homogenates of rat embryos obtained on the 14th day of gestation and were found to be different than those reported for adult tissues. 2. Measurements of various organelle reference enzyme activities on the 14th and 15th days of gestation revealed a pattern of selective rates of subcellular organelle biogenesis during this period: lysosomes greater than mitochondria greater than endoplasmic reticulum = plasma membranes.
D000375:Aging; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005865:Gestational Age; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D009708:Nucleotidases / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D009708:Nucleotidases; D010727:Phosphoric Diester Hydrolases; D008274:Magnesium; D002118:Calcium