title: Exercise and the cAMP system in rat adipose tissue. I. Lipid mobilization.
abstract: Protein kinase (PK) and hormone-sensitive lipase (HSL) were measured at rest in adipose tissue of untrained male rats and in that of animals subjected to a strenuous program of treadmill running. Total amounts of PK activity (decreased from 860 +/- 104 to 474 +/- 53 pmol.min-1. (10(6) cells)-1 (P less than 0.01) as a result of exercise training. At the same time, binding capacity for adenosine 3',5'-cyclic monophosphate (cAMP) was elevated in the runners. These data suggest a functional loss in catalytic activity without a loss in binding capacity. In addition, the results provide evidence that the capacity of PK to activate HSL is reduced in adipocytes of physically trained rats. HSL activity was measured in both adipose tissue slices and isolated adipocytes. The results show that the levels of activity of HSL did not increase as a result of the running program. These results provide evidence that the lipolytic capacity of adipocytes of normal untrained male rats is sufficiently large to meet the increased demand for free fatty acids imposed by the exercise program without the need for an adaptive increase in HSL activity.
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006801:Humans; D008049:Lipase / Q000378:metabolism; D008053:Lipid Mobilization / Q000187:drug effects; D005082:Physical Exertion / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats
chemical_list: D005230:Fatty Acids, Nonesterified; D000242:Cyclic AMP; D011494:Protein Kinases; D008049:Lipase; D004837:Epinephrine