Steady-state kinetics of mouse DNA polymerase alpha.
Steady-state kinetic studies of DNA polymerase alpha purified from mouse myeloma MOPC104E cells have been carried out. The results of initial velocity analysis with or without sodium pyrophosphate, a product inhibitor, indicated that the reaction mechanism of this enzyme can be categorized as an ordered Bi Bi type where the concentration of the ternary complex is very low.
D000818:Animals; D002460:Cell Line; D004257:DNA Polymerase II / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D011756:Diphosphates / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010954:Plasmacytoma
D011756:Diphosphates; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase