Synthesis of a collagenase inhibitor by gingival fibroblasts in culture.
Human collagenase was inhibited by test solutions of human gingival fibroblast culture media. The fibroblast-derived collagenase inhibitor was only slightly affected by 10 micrograms trypsin but was inactivated with 100 micrograms trypsin. The chaotropic agent KSCN (3 M) completely inactivated the inhibitor, whereas the thiol-blocking reagent, p-aminophenylmercuric acetate, partially inactivated the inhibitor. Inhibitory activity was retained at 60 degrees C but was abolished at 100 degrees C. Following ammonium sulfate fractionation, the fibroblast inhibitor was recovered in the supernatant at concentrations of at least 70% saturation. It is suggested that collagenase latency in soft connective tissues may derive from a collagenase-inhibitor complex formed by interaction of collagenase and a fibroblast-derived inhibitor.
D002460:Cell Line; D002850:Chromatography, Gel; D005347:Fibroblasts / Q000378:metabolism; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors*; D008970:Molecular Weight
D003012:Microbial Collagenase