title: Restriction endonuclease BamHI cleaves bacteriophage Mu DNA within cistrons E and F.
abstract: We have cleaved phage Mu DNA with restriction endonucleases EcoRI and BamHI and have cloned three specific DNA fragments from the middle of the Mu genome into vector plasmid pBR322. By marker rescue experiments, we have determined that the two BamHI cleavage sites in Mu DNA occur within cistrons E and F.
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes