Maternal inheritance of mitochondrial DNA polymorphisms in cultured human fibroblasts.
We have isolated the total cellular DNA from the cultured diploid fibroblasts of a six-member, three-generation human family. Using a specific radioactive probe for mitochondrial (mt) sequences we have identified new polymorphic variants in this family for the Hhal restriction endonuclease cleavage pattern of the mtDNA. The inheritance of these cleavage patterns verifies the maternal inheritance of mtDNA through all three generations.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002648:Child; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D005260:Female; D006801:Humans; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D012867:Skin / Q000166:cytology; D013379:Substrate Specificity
D004272:DNA, Mitochondrial; D004247:DNA; D004262:DNA Restriction Enzymes