Resolution of RS-abscisic acid and the separation of abscisic acid metabolites from plant tissue by high-performance liquid chromatography.
Attempts to resolve the enantiomers of racemic abscisic acid (ABA) by high-performance liquid chromatography on a chiral stationary-phase column were unsuccessful. However, reduction of RS-methyl ABA (RS-Me-ABA) with sodium borohydride generates a new chiral centre and one of the two isomeric products, the RS-Me-1',4'-cis-diol of ABA, was separated into its enantiomers by high-performance liquid chromatography on an optically active Pirkle column. High-performance liquid chromatography on a mu Bondapak C18 column separated the metabolites and conjugates of [2-14C]ABA fed to tomato shoots. The resolution method was used to measure the relative proportions of R and S enantiomers in the free acid liberated from conjugates of ABA.
D000040:Abscisic Acid / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003509:Cyclohexanecarboxylic Acids / Q000302:isolation & purification*; D010937:Plant Growth Regulators / Q000302:isolation & purification*; D014675:Vegetables / Q000032:analysis
D003509:Cyclohexanecarboxylic Acids; D010937:Plant Growth Regulators; D000040:Abscisic Acid