["Pharaoh" line culture of Japanese quail cells as a leukosis-free system for virus reproduction].
Contamination of Japanese quail, strain Pharaoh, cell culture with oncogenous and infectious avian viruses was studied. The susceptibility of the embryonal cell cultures of the Japanese quail, strain Pharaoh, to measles, parotitis and fixed rabies viruses was also determined. It was found that the sera of pubertal quails had no antibody to Rous sarcoma virus (RSV), strains Brian, RSV (RAV-1), Schmidt-Ruppin, Carr-Zilber, as well as to Marek's disease and Newcastle disease viruses. No reverse transcriptase activity was detected in the embryonal alantoic fluid of this avian species. The quails were less susceptible, as compared to the chicken, to Schmidt-Ruppin and Carr-Zilber strains of RSV. Measles, parotitis and fixed rabies viruses reproduced actively in the Japanese quail, Pharaoh strain, embryonal cell cultures. It is suggested that the embryonal cell cultures of this avian species can be used as a leukemia-free substrate for experimental studies and manufacturing of viral vaccines.
D001355:Alpharetrovirus / Q000302:isolation & purification*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000472:pathogenicity; D002478:Cells, Cultured; D002645:Chickens; D003370:Coturnix / Q000196:embryology / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology; D008459:Measles virus / Q000254:growth & development; D009109:Mumps virus / Q000254:growth & development; D011820:Rabies virus / Q000254:growth & development; D013045:Species Specificity; D014776:Virus Cultivation
Kul'tura kletok iaponskikh perepelov linii faraon kak bezleĭkoznaia sistema dlia reproduktsii virusov.