[Effect of periodate oxidation on properties of antibodies].
The subject of the present research is the investigation of the influence of sodium periodate on the properties of immunoglobulin G molecules. It is shown that 100 and 300 M of periodate cause a slight enhancement of the sedimentation coefficient which is a result of the higher protein density. However high concentrations (2000 M) of periodate decrease sedimentation coefficient considerably and disturb the protein structure homogeneity. Studies of the immunologic activity in the periodate-treated antibodies by the reaction of passive hemagglutination showed that in low concentrations it did not decrease significantly the activity but with an increase in the concentration up to 2000 M the activity lowered. The conjugation of antibodies with the enzyme markers was fulfilled due to periodate oxidation. The conjugates obtained were successfully used for improving sensitivity of the precipitation line in immunologic tests.
D000818:Animals; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011817:Rabbits / Q000276:immunology; D051381:Rats / Q000276:immunology
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D010504:Periodic Acid
Vliianie perĭodatnogo okisleniia na svoĭstva antitel.