Gradation of the magnitude of the electrochemical proton gradient in Mycoplasma cells.
The results presented show that in Mycoplasma mycoides var. Capri, regulation of glucose uptake by its non-metabolizable analogue methyl alpha-D-glucoside, can be used to control intracellular ATP content. This in turn leads to a control of the rate of proton extrusion catalysed by the Mg2+-dependent ATPase (phi (cHxN)2C H+) and the respective amplitudes of the components of delta mu H+. When Mycoplasma cells are incubated with 10 mM methyl alpha-D-glucoside, the amplitude of phi (cHxN)2C H+, of the electrical potential delta psi and of the chemical gradient delta pH become continuous functions of external glucose concentration within the limits of the non-energized and fully energized states. Analysis of the relationships between graduated amplitudes of delta psi, delta pH and phi (cHxN) 2C H+ show that the primary form of energy stored by a delta  mu H+ generator is the electrical potential.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001693:Biological Transport, Active; D004563:Electrochemistry; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000187:drug effects; D008757:Methylglucosides / Q000494:pharmacology; D009176:Mycoplasma mycoides / Q000378:metabolism*; D011522:Protons
D008757:Methylglucosides; D011522:Protons; C027020:methylglucoside; D000255:Adenosine Triphosphate; D005947:Glucose