title: Separation between modulator-dependent protein kinases I and II.
abstract: The separation of modulator-dependent protein kinase I from modulator-dependent protein kinase II obtained from the lungs of sexually premature male mice was accomplished by Sephadex G-200 gel filtration. After preincubation of a mouse lung cytosol fraction with arginine-rich histone, theophylline, cyclic GMP and crude protein kinase modulator a cyclic GMP-dependent protein kinase activity peak present in a non-preincubated sample completely disappeared and was replaced by a late-eluted modulator-dependent protein kinase II peak. There was a difference in substrate specificity between modulator-dependent protein kinase I and modulator-dependent protein kinase II despite their similar dependence on crude protein kinase modulator or partially purified stimulatory protein kinase modulator for their maximal activities.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008168:Lung / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D011479:Protamines; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D002352:Carrier Proteins; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D011479:Protamines; C015482:protein kinase modulator; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP