title: The prosthetic groups in succinate dehydrogenase. Number and stoichiometry.
abstract: I. Succinate:Q oxidoreductase (EC 1.3.99.1) as present in beef-heart submitochondrial particles contains equal amounts of FAD, a [2Fe-2S] cluster and a [4Fe-4S] cluster. Both Fe-S clusters are reducible by succinate. 2. A second type of [2Fe-2S] cluster, called center S-2, that has been proposed to be present in purified preparations of succinate dehydrogenase and isolated Complex II (Ohnishi, T., Winter, D.B., Lim, J. and King, T.E. (1973) Biochem. Biophys. Res. Commun. 53, 231--237) is an artifact introduced by the purification procedure. 3. It is suggested that the 70 000 dalton subunit which is known to bind the flavin, accomodates also the [4Fe-4S] cluster whereas the 28 000 dalton subunit contains the [2Fe-2S] cluster.
mesh_terms: D000818:Animals; D002417:Cattle; D004227:Dithionite / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005182:Flavin-Adenine Dinucleotide / Q000032:analysis*; D007501:Iron / Q000032:analysis*; D007506:Iron-Sulfur Proteins / Q000032:analysis; D008929:Mitochondria, Heart / Q000201:enzymology*; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000494:pharmacology; D013367:Submitochondrial Particles / Q000201:enzymology; D013384:Succinate Cytochrome c Oxidoreductase / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis*
chemical_list: D007506:Iron-Sulfur Proteins; D005182:Flavin-Adenine Dinucleotide; D004227:Dithionite; D007501:Iron; D013384:Succinate Cytochrome c Oxidoreductase; D013385:Succinate Dehydrogenase; D011190:Potassium Cyanide