A DNA-binding protein specific for the early replicated region of the chromosome obtained from Escherichia coli membrane fractions.
After extensive dialysis of Escherichia coli membranes treated with Triton X-100, three membrane proteins (A, B and B') with an affinity for DNA have been isolated and purified. They bind to either double-stranded or single-stranded DNA. A and B' proteins preferentially attach to DNA even in the presence of poly(uridylic acid). Only protein B' can recognize some base sequence of DNA because pulse-labelled DNA made at the initiation of replication in a synchronized dnaC mutant has been selectively retained by the protein.
D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004265:DNA Helicases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D017830:Octoxynol; D011072:Poly U / Q000494:pharmacology; D011092:Polyethylene Glycols; D012965:Sodium Chloride / Q000494:pharmacology
D011072:Poly U; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017830:Octoxynol; D004265:DNA Helicases; D008274:Magnesium; D002118:Calcium