Promoter sequences of eukaryotic protein-coding genes.
In vitro genetic techniques were used to study the sequence requirements for the initiation of specific transcription. Deletion mutants were constructed around the putative promoter of the adenovirus-2 major late and chicken conalbumin genes. Specific transcription in vitro by RNA polymerase B together with a HeLa cell cytoplasmic extract was used as the test for promoter function. With this approach sequences which are essential for the initiation of specific transcription in vitro, were shown to be located between 12 and 32 base pairs upstream from the 5' end of these genes.
D001483:Base Sequence; D001665:Binding Sites; D002468:Cell Physiological Phenomena; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000502:physiology*; D009876:Operon; D012319:RNA Polymerase II / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D004247:DNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes