title: [Action of dicaine on the efficiency of plasmid transformation and transfection in Escherichia coli K12].
abstract: The action of tetracaine hydrochloride, a local anesthetic, on the effectiveness of plasmid transformation and transfection in variants of E. coli K-12 has been studied. The concentrations of tetracaine hydrochloride used in the experiment (0.002 M) affect the level of the synthesis of most proteins in the outer membrane of these bacteria. This seems to be one of the causes of changes in the effectiveness of plasmid transformation and transfection in E. coli K-12.
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics; D002462:Cell Membrane / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D009154:Mutation; D010957:Plasmids / Q000187:drug effects*; D013748:Tetracaine / Q000494:pharmacology*; D014162:Transfection / Q000187:drug effects*; D014169:Transformation, Bacterial / Q000187:drug effects*
chemical_list: D000779:Anesthetics, Local; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D013748:Tetracaine
vernacular_title: Deĭstvie dikaina na éffektivnost' plazmidnoĭ transformatsii i transfektsii Escherichia coli K12.