Porcine rotaviral infection of cell culture: effects of certain enzymes.
Porcine rotaviral infectivity for continuous porcine kidney (PK-15) cells was enhanced by incorporation of pancreatic endopeptidases into the cell culture maintenance medium. Marked enhancement of infectivity was induced by trypsin, whereas elestase and alpha-chymotrypsin enhanced infectivity to a lesser extent. Bacterial protease also induced some enhancement of porcine rotaviral infectivity. A synergistic enhancement of porcine rotaviral infectivity was noticed with trypsin and alpha-chymotrypsin combined. Porcine rotaviral infectivity was not affected by incorporation of alpha-amylase, alkaline phosphatase, beta-galactosidase, carboxypeptidase-A, deoxyribonuclease, enterokinase, lipase, or ribonuclease into the maintenance medium.
D000818:Animals; D002478:Cells, Cultured; D002918:Chymotrypsin / Q000494:pharmacology; D005696:Galactosidases / Q000494:pharmacology; D006867:Hydrolases / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000494:pharmacology; D012328:RNA Viruses / Q000254:growth & development*; D012401:Rotavirus / Q000187:drug effects / Q000254:growth & development*; D013552:Swine / Q000382:microbiology
D006867:Hydrolases; D005696:Galactosidases; D002918:Chymotrypsin; D010196:Pancreatic Elastase