Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum.
The transposon A sequence Tn1 containing the ampicillin resistance determinants was transposed from RP4 to a plasmid of the marine fish pathogen Vibrio anguillarum. Curing experiments in which plasmid loss was determined by analysis of the segregation of the ampicillin resistance phenotype showed the association of virulence with the specific V. anguillarum plasmid class.
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D004251:DNA Transposable Elements; D005393:Fish Diseases / Q000382:microbiology*; D010957:Plasmids; D012474:Salmon / Q000382:microbiology*; D014733:Vibrio / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*
D004251:DNA Transposable Elements; D000667:Ampicillin