Construction of an SV40-derived cloning vector.
A new reiterated variant of simian virus 40 (SV40; dl1142) has been constructed. It should be useful for the purpose of cloning foreign pieces of DNA in SV40 virions. Up to 80% of the SV40 genome has been made available for substitution with foreign DNA and the vector contains a number of unique (single-cut) restriction sites which will facilitate cloning. The 5' and 3' regions of both the SV40 early and late messenger RNAs are included in the vector. Prokaryotic DNA has been successfully cloned in the early region of the vector. The transcriptional properties of the recombinant have been studied, and it was found that both the vector and insert DNA are transcribed, mainly as non-adenylated RNA.
D003001:Cloning, Molecular; D014644:Genetic Variation; D005822:Genetic Vectors; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic