Increased carbon tetrachloride-stimulated chemiluminescence in the in situ liver of barbital-treated mice.
Mice treated with barbital (0.1% in the drinking water) during 15 days showed a 63% increased endoplasmic reticulum mass. The carbon tetrachloride-stimulated chemiluminescence of the in situ liver was 51% increased after barbital treatment. Hydroperoxide-stimulated chemiluminescence of liver homogenates and microsomal suspensions were increased by 140 and 92%, respectively, in the barbital-treated mice. Spontaneous liver chemiluminescence (109 cps/cm2) was found unchanged after barbital treatment. Superoxide dismutase, catalase and glutathione peroxidase activities were 109, 61 and 103%, respectively, increased after barbital treatment. The results are consistent with a primary role of cytochrome P 450 in the biotransformation of CCl4, which initiates a radical chain reaction leading to the production of powerful oxidizing species. Apparently, superoxide dismutase, catalase and glutathione peroxidase are synthetized in a coincident manner with respect to cytochrome P 450.
D000818:Animals; D001462:Barbital / Q000627:therapeutic use*; D001463:Barbiturates / Q000627:therapeutic use*; D002251:Carbon Tetrachloride / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D004307:Dose-Response Relationship, Radiation; D004721:Endoplasmic Reticulum / Q000502:physiology*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000502:physiology*; D008163:Luminescent Measurements; D051379:Mice; D008862:Microsomes, Liver / Q000502:physiology; D009929:Organ Size; D013347:Subcellular Fractions; D013482:Superoxide Dismutase / Q000378:metabolism
D001463:Barbiturates; D001462:Barbital; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione