Ultrastructure of GABA-neurons in cultures of rat neostriatum.
The subcellular distribution of glutamate decarboxylase (GAD) was revealed by electron microscopic immunocytochemistry in primary cultures of the rat neostriatum. Immunoreactivity was restricted to a small nerve cell type with rich amounts of free ribosomes and an indented nucleus. Glial cells were non-reactive. The results indicate that GABA-synthesizing neurons are viable in culture conditions and suggest that neostriatal GABA-neurons are small cells with simple ultrastructure since larger cells did not possess immunoreactivity.
D000818:Animals; D003342:Corpus Striatum / Q000033:anatomy & histology* / Q000201:enzymology; D046508:Culture Techniques; D005968:Glutamate Decarboxylase / Q000378:metabolism; D008854:Microscopy, Electron; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase