title: Localization of the thymidine kinase gene of herpes simplex virus type 2 (333).
abstract: The thymidine kinase (tk) gene of herpes simplex virus type 2 (HSV-2) has been identified on purified restriction endonuclease fragments of HSV-2 DNA. These fragments were localized on the physical map of HSV-2 DNA. A detailed map of the HSV-2 tk gene region has been constructed which locates the tk gene between 0.285 and 0.310 map units [3.8 kilobase pairs (kb)], whereby tk gene sequences are at least present between 0.299 and 0.303 map units (0.6 kb). Mouse tk- cells that were biochemically transformed contain HSV-2 tk gene DNA sequences that are integrated in the cellular genome.
mesh_terms: D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes