Isolation of identification of 24(R)-hydroxyvitamin D3 from chicks give large doses of vitamin D3.
A new metabolite of vitamin D was isolated from the blood plasma of chicks given large doses of vitamin D3. The isolation involved methanol-chloroform extraction and four column chromatographic steps. The metabolite was identified by high- and low-resolution mass spectroscopy, chemical derivatization, an comigration with authentic standard as 3 beta, 24(R)-dihydroxy-9,10-seco-5,7,10(19)-cholestatriene [24(R)-hydroxyvitamin D3]. No detectable 24-(R)-hydroxyvitamin D3 was recovered from 16 L of plasma from chicks receiving physiologic levels of vitamin D3.
D015650:24,25-Dihydroxyvitamin D 3; D000818:Animals; D002645:Chickens; D002762:Cholecalciferol / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006887:Hydroxycholecalciferols / Q000097:blood / Q000302:isolation & purification*; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D006887:Hydroxycholecalciferols; C030711:24-hydroxyvitamin D3; D002762:Cholecalciferol; D015650:24,25-Dihydroxyvitamin D 3