Colony growth in soft agar of human melanoma, sarcoma, and lung carcinoma cells disaggregated by mechanical and enzymatic methods.
The effect of mechanical and enzymatic disaggregation on human malignant melanoma, soft-tissue sarcoma and lung carcinoma colony growth in soft agar was studied. The enzymatic disaggregation was advantageous in most cases of melanoma and sarcoma, giving a larger number of colonies and increasing the probability of achieving growth in soft agar. Enzymatically treated pulmonary carcinoma cell populations had lower clonogeneic potential, especially in the case of anaplastic carcinomas. Morphological studies showed that the cells growing in soft-agar colonies had the same characteristics as those of the original tumor. A linear relationship was obtained between the number of enzymatically and mechanically treated tumor cells plated and the number of colonies. Delayed plating decreased the number of colonies.
D000362:Agar; D002277:Carcinoma / Q000473:pathology*; D002449:Cell Aggregation; D002999:Clone Cells; D003584:Cytological Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008545:Melanoma / Q000473:pathology*; D003012:Microbial Collagenase / Q000494:pharmacology; D012509:Sarcoma / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000473:pathology; D013997:Time Factors
D000362:Agar; D003012:Microbial Collagenase