Quantitative microautoradiography of arteries: comparison of radioactivity to silver.
The local concentration of silver in developed aortic transmural microautoradiographs was compared to the corresponding 125I-labeled albumin radioactivity concentration [ci(x)] in the subjacent tissue. Silver [s(x)] was measured by electron probe X-ray microanalysis (EPA) and the corresponding ci(x) by direct gamma-ray counting. The results show 1) that the relationship between volume-averaged values of radioactivity (ci) and EPA signal (s) is adequately described by ci = Ks, where K is a proportionality constant, and 2) that ci(x) measured by EPA [i.e., Ks(x)] agrees closely with ci(s) measured direcly from en face microtomy slices of corresponding unfixed tissue specimens.
D000818:Animals; D001158:Arteries / Q000378:metabolism*; D001345:Autoradiography; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy; D006651:Histocytochemistry; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D012834:Silver
D007457:Iodine Radioisotopes; D012709:Serum Albumin; D012834:Silver