[Transformation and transduction in Bacillus subtilis strains with the BsuR restriction-modification system by modified and unmodified pUB110 plasmid DNA].
Plasmid pUB110 DNA is restricted and modified during transformation of Bacillus subtilis cells possessing the BsuR system of restriction-modification. Restriction has comparatively little influence on the frequency of plasmid transformation only reducing it 20 times. The frequency of transduction of nonmodified plasmid DNA into modifying recipient cells, using phage Ar9 is also reduced a little. The frequency of transduction of chromosomal markers is much more lowered under the same experimental conditions.
D001412:Bacillus subtilis / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005819:Genetic Markers; D010957:Plasmids; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005819:Genetic Markers; D004262:DNA Restriction Enzymes
Transformatsiia i transduktsiia u shtammov Bacillus subtilis s sistemoĭ restriktsii-modifikatsii BsuR posredstvom modifitsirŏvannoĭ nemodifitsirovannoĭ DNK plazmidy pUB110.