title: Structure of nucleosome core particles containing uH2A (A24).
abstract: We have purified uH2A (A24) and reconstituted it, in place of H2A, into high molecular weight nucleohistone containing the core histones and DNA. uH2A-containing core particles were then prepared by nuclease digestion and studies on these particles were carried out. We show that two uH2A molecules can be accommodated within a core particle. We also show that the presence of two uH2A molecules in a core particle does not alter significantly either the pattern or the rate of DNase I digestion as compared to both reconstituted and native core particles. Finally, we show that HMG proteins 14 and 17 can bind to uH2A-containing core particles. We conclude that uH2A has little influence on structure at the level of individual nucleosomes.
mesh_terms: D000818:Animals; D002417:Cattle; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases; D004720:Endonucleases; D006609:High Mobility Group Proteins; D006657:Histones / Q000032:analysis*; D008836:Micrococcal Nuclease; D008970:Molecular Weight; D009707:Nucleosomes / Q000648:ultrastructure*; D013950:Thymus Gland / Q000032:analysis
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D006657:Histones; D009707:Nucleosomes; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease