Serum 5'-nucleotidase of a breast cancer patient.
Serum derived from the breast cancer patient included in this study was found to be a rich source of 5'-nucleotidase. In addition, it also contains nonspecific alkaline phosphatase. The properties of 5'-nucleotidase were studied by eliminating the interference of serum non-specific alkaline phosphatase by the preliminary incubation of serum in glycine-NaOH buffer containing ethylenediamine tetraacetate and magnesium. The enzyme has a pH optimum at 9.5. It is remarkably stable when held at 50 degrees C at pH 7.5, but it readily lost activity in the acid medium at pH 4.2. It hydrolyzes both the ribo- and deoxyribo-nucleoside 5'-phosphates. It has the highest preference for cytidine 5'-phosphate. Adenosine 2'- or 3'- phosphates are refractory to its action.
D015720:5'-Nucleotidase; D000249:Adenosine Monophosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D001943:Breast Neoplasms / Q000201:enzymology*; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008875:Middle Aged; D009708:Nucleotidases / Q000097:blood*; D013379:Substrate Specificity
D000249:Adenosine Monophosphate; D004492:Edetic Acid; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D008274:Magnesium