A technique to detect the reaction between poliovirus structural polypeptides and neutralizing anti-polio IgG.
Electrophoretically separated poliovirus capsid proteins were blotted on nitrocellulose membranes. A method for the immunological identification of the blotted polypeptides was developed. No reaction could be detected by this method between individualized poliovirus structural polypeptides and neutralizing anti-polio antibodies.
D000818:Animals; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G; D010455:Peptides / Q000032:analysis; D017955:Poliovirus / Q000032:analysis*; D011817:Rabbits; D014764:Viral Proteins; D014771:Virion / Q000648:ultrastructure*
D007074:Immunoglobulin G; D010455:Peptides; D014764:Viral Proteins