Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry.
Benzodiazepine receptors mounted tissue sections of brain were localized by autoradiography using a tritium-sensitive sheet film (3H-Ultrofilm, LKB). The use of the film had many advantages compared to a previous technique utilizing emulsion-coated coverslips. A major advantage is the adaptability of the film to microdensitometric techniques for measurement of receptors. The densitometry can be carried out by computerized, color-coded image analysis.
D000818:Animals; D001345:Autoradiography; D001569:Benzodiazepines / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004655:Emulsions; D005445:Flunitrazepam / Q000378:metabolism; D008722:Methods; D051381:Rats; D011955:Receptors, Drug / Q000378:metabolism*; D011963:Receptors, GABA-A; D014316:Tritium
D004655:Emulsions; D011955:Receptors, Drug; D011963:Receptors, GABA-A; D014316:Tritium; D001569:Benzodiazepines; D005445:Flunitrazepam