Radioprotection of bovine erythrocytes to haemolysis.
The effect of several free-radical scavengers on the radiation-induced haemolysis of bovine erythrocytes (50 krad, 60Co, cell concentration: 0.05 v/v) was studied. A two-phase effect of the additives was found with a maximum of the protective action in the millimolar concentration range. A correlation was established between the degree of protection at a fixed concentration (5mM) and the rate constants of reactions of respective compounds with OH. A radiosensitizing effect of diethyldithiocarbamate, an inhibitor of superoxide dismutase (SOD) was not confirmed and no specific protective effect of SOD was seen.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002417:Cattle; D003037:Cobalt Radioisotopes; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004912:Erythrocytes / Q000528:radiation effects*; D005609:Free Radicals; D006461:Hemolysis; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013890:Thiourea / Q000494:pharmacology
D001386:Azides; D003037:Cobalt Radioisotopes; D005609:Free Radicals; D011837:Radiation-Protective Agents; D004050:Ditiocarb; D013890:Thiourea; D004121:Dimethyl Sulfoxide