Characterization and localization of atriopeptin in rat atrium.
An antiserum specific for atriopeptin was used to characterize and localize atriopeptin-like immunoreactive material in rat atrium by radioimmunoassay and immunohistochemical techniques. The antiserum recognizes atriopeptin I, atriopeptin III, and alpha-human atrial natriuretic polypeptide, but does not recognize met-enkephalin, cholecystokinin, dynorphin A, bradykinin, substance P, or beta-endorphin. A high content of atriopeptin was found in crude extracts of rat atria, as compared to ventricles, and the atriopeptin-like immunoreactive material was found to be located exclusively in granules within atrial cardiocytes. Fractionation of the immunoreactive material by gel filtration and reverse-phase HPLC revealed the presence of multiple atriopeptins.
D000818:Animals; D000918:Antibody Specificity; D009320:Atrial Natriuretic Factor; D006325:Heart Atria / Q000378:metabolism*; D006651:Histocytochemistry; D007120:Immunochemistry; D009124:Muscle Proteins / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D009124:Muscle Proteins; D009320:Atrial Natriuretic Factor