A high-pressure-liquid-chromatography method for the determination of corticosterone in rat plasma.
A high-pressure-liquid-chromatography (HPLC), isotope dilution method for the determination of corticosterone in rat plasma is reported. Main characteristics of the technique are: efficient separation of the steroid from 18-HO-DOC and 11-deoxycortisol; two easy pre-purification steps consisting of an acetone extraction-deproteinization and fractionation on Sephadex LH-20; a sensitivity of 5 ng per injection and the possibility of determining 25 ng corticosterone in approximately one milliliter rat plasma.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000097:blood*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D003345:Corticosterone