Lysosomal enzyme activities and low density lipoprotein receptors in circulating mononuclear cells. Effect of insulin therapy in diabetic patients.
Methods for the measurement of the lysosomal enzymes acid cholesterol-ester hydrolase and N-acetyl-beta-glucosaminidase were adapted for use with freshly isolated circulating mononuclear cells. Activities of both these enzymes increased (to 259 and 147% of control values respectively) after 7 days of insulin therapy in diabetic subjects. Low density lipoprotein degradation by freshly isolated mononuclear cells increased simultaneously by 67% (p less than 0.05). The findings in this pilot study suggest [1] that circulating mononuclear cells can be used to evaluate the effect of metabolic changes in vivo on lysosomal enzymes and low density lipoprotein metabolism, and [2] that insulin can stimulate cellular lysosomal enzyme activity.
D000118:Acetylglucosaminidase / Q000097:blood*; D000328:Adult; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood* / Q000188:drug therapy; D005260:Female; D006596:Hexosaminidases / Q000097:blood*; D006801:Humans; D007328:Insulin / Q000627:therapeutic use*; D008077:Lipoproteins, LDL / Q000097:blood*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000097:blood*; D011973:Receptors, LDL; D002787:Sterol Esterase / Q000097:blood*
D007328:Insulin; D008077:Lipoproteins, LDL; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase