Alpha-1-antitrypsin in intracellular inclusions of diethylnitrosamine induced hepatomas of C57BLxC3H F1 mice.
Six large hepatic nodules obtained 46 to 74 weeks after a single dose of diethylnitrosamine (0.625 or 1.25 micrograms/gm) in infant C57BLxC3H F1 male mice were studied to elucidate the nature of intracytoplasmic inclusions in the hepatic cells of these nodules. By light microscopy, the nodules constituted a spectrum of lesions ranging from some which were well differentiated adenomas to others which were frank carcinomas. Round or oval eosinophilic intracytoplasmic inclusions were consistently seen in the well differentiated adenomatous lesions. Some of these inclusions were PAS positive and resisted diastase digestion while others were either negative or only weakly PAS positive. By electron microscopy, they were most commonly crystalline, contained a reticulated electron dense material and were located in the distended rough endoplasmic reticulum. On immunofluorescence the inclusions showed marked specific reactivity with antisera against human alpha-1-antitrypsin. The presence of alpha-1-antitrypsin in these inclusions was further supported by the resistance of these inclusions to digestion by trypsin or papain but not by the pepsin or pronase.
D000818:Animals; D004052:Diethylnitrosamine / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D002479:Inclusion Bodies / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis* / Q000139:chemically induced / Q000473:pathology; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009602:Nitrosamines / Q000494:pharmacology*; D010447:Peptide Hydrolases; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D009602:Nitrosamines; D000515:alpha 1-Antitrypsin; D004052:Diethylnitrosamine; D010447:Peptide Hydrolases