[Detection of a paramagnetic product developing during oxidative phosphorylation in mitochondria].
It was shown by ESR technique using flow system combined with ESR-spectrometer that paramagnetic product appearing in the course of oxidative phosphorylation was directly associated with mitochondrial ATPase operation. A decrease of ESR signal intensity and the changes of its form observed on mitochondria uncoupled by 2,4-dinitrophenol as compared with those inhibited by olygomicin suggest that in the case of olygomycin block a free radical ATPase linked intermediate can be recorded, the ESR signal of which seems to be partly due to flavin semiquinone of ATPsynthetase itself.
D025181:ATP Synthetase Complexes; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*
D004140:Dinitrophenols; D005609:Free Radicals; D009097:Multienzyme Complexes; D009840:Oligomycins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D025181:ATP Synthetase Complexes
Obnaruzhenie paramagnitnogo produkta, voznikaiushchego pri okislitel'nom fosforilirovanii v mitokhondriiakh.