Activation of phosphodiesterase in frog rod outer segment by an intermediate of rhodopsin photolysis I.
Guanosine 3',5'-cyclic monophosphate phosphodiesterase (EC 3.1.4.1) in frog rod outer segment prepared by a sucrose stepwise density gradient method was activated by light in the presence of GTP. Rhodopsin in rod outer segment was solubilized with sucrose laurylmonoester and then purified by concanavalin A-Sepharose column. Addition of photo-bleached preparation of the purified rhodopsin to the crude rod outer segment, which had been prepared by 43% (w/w) sucrose floatation, caused the activation of phosphodiesterase in the dark, while each component of the photo-product eluted from the column (all-trans retinal and opsin) did not. Regenerated rhodopsin prepared from 11-cis retinal and purified opsin activated phosphodiesterase when it was bleached. From these facts it is suggested that an intermediate or a process of photolysis of rhodopsin causes activation of phosphodiesterase.
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004789:Enzyme Activation; D017830:Octoxynol; D010775:Photic Stimulation; D010782:Photolysis; D010786:Photoreceptor Cells / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011892:Rana catesbeiana / Q000378:metabolism*; D012168:Retinal Pigments / Q000494:pharmacology*; D012243:Rhodopsin / Q000032:analysis / Q000494:pharmacology*; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D013395:Sucrose / Q000031:analogs & derivatives / Q000494:pharmacology
D012168:Retinal Pigments; C018152:sucrose monolaurate; D003208:Concanavalin A; D011092:Polyethylene Glycols; D013395:Sucrose; D017830:Octoxynol; D012243:Rhodopsin; D015106:3',5'-Cyclic-GMP Phosphodiesterases