title: Receptors for multiplication-stimulating activity on human arterial and venous endothelial cells.
abstract: Primary cultures of endothelial cells were prepared from the arteries and veins of human umbilical cords. Both arterial and venous endothelial cells demonstrated specific receptors for the insulin-like growth factor MSA (Multiplication-Stimulating Activity). Insulin, at concentrations up to 10(-7) M, had little effect on 125I-MSA binding whereas MSA was congruent to 1% as potent as insulin in competing with 125I-insulin binding. Serum containing anti-insulin receptor antibodies blocked binding of 125I-insulin to the endothelial cells but had little effect on 125I-MSA binding. We conclude that human endothelial cells have specific and distinct receptors for both MSA and insulin.
mesh_terms: D001667:Binding, Competitive; D004727:Endothelium / Q000378:metabolism; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007335:Insulin-Like Growth Factor II; D007700:Kinetics; D008934:Mitogens / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017451:Receptors, Somatomedin; D014469:Umbilical Arteries / Q000378:metabolism*; D014471:Umbilical Veins / Q000378:metabolism*
chemical_list: D007328:Insulin; D008934:Mitogens; D010455:Peptides; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D007335:Insulin-Like Growth Factor II