Separation of cytotoxic leukocyte populations of human peripheral blood and colostrum of PVP-silica (Percoll) density gradients.
PVP-silica (Percoll) did not inhibit antibody-dependent cellular cytotoxicity in an 18 h 51Cr-release assay. By the use of a simple discontinuous density gradient centrifugation technique, leukocytes from peripheral blood and colostrum were separated into morphologic and cytotoxic functional populations. The major cytotoxic effector cells in colostrum are lipid-free macrophages and probably a small lymphocyte subpopulation.
D000920:Antibody-Dependent Cell Cytotoxicity; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D003102:Colloids; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D011205:Povidone; D012822:Silicon Dioxide
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone