Pyrimidine metabolism of Bdellovibrio bacteriovorus grown intraperiplasmically and axenically.
Bdellovibrio bacteriovorus grown axenically or intraperiplasmically on Escherichia coli has pathways for the interconversion of pyrimidines and the synthesis of pyrimidine nucleoside 5'-triphosphates similar to those found in the enteric bacteria. Minimal differences in enzyme activities were observed for axenically and intraperiplasmically grown cells. As might be expected for an organism which takes up deoxyribonucleoside 5'-monophosphates per se, high levels of enzymes which catalyze the generation of deoxyribonucleoside triphosphates from monophosphates were found. In addition, all enzymes of the thymine salvage pathway, except for thymidine kinase, were directly demonstrated in wild-type strains. It was possible to demonstrate this activity only indirectly owing to an inhibitor in wild-type extracts. Investigations with inhibitors of pyrimidine interconversion reactions showed that essentially all B. bacteriovorus deoxyribonucleic acid not synthesized from units derived from E. coli deoxyribonucleic acid is made from components of the substrate organism's ribonucleic acid. Evidence for de novo pyrimidine synthesis from the amino acid level was not found for B. bacteriovorus grown on E. coli that had a high protein/deoxyribonucleic acid ratio or on normal E. coli. The potential for de novo pyrimidine synthesis by intraperiplasmically grown B. bacteriovorus, however, cannot be totally ruled out on the basis of these investigations.
D001501:Bdellovibrio / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D005467:Floxuridine / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism
D011743:Pyrimidines; D005467:Floxuridine; D010770:Phosphotransferases; D013937:Thymidine Kinase; D008727:Methotrexate