Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins.
Cells nonproductively transformed by the Snyder-Theilen, Gardner-Arnstein, and McDonough strains of feline sarcoma virus synthesize gag-x polyproteins of 78,000, 100,000, and 180,000 daltons, respectively. These feline sarcoma virus-coded products were precipitated by antisera to polypeptides encoded by the gag gene of feline leukemia virus and by rat antisera raised to feline sarcoma virus-transformed rat tumor cells. Precipitation with rat antisera absorbed with feline leukemia virus showed that the x-portions of the three gag-x proteins were each antigenically distinct, suggesting that the src genes of the three independent isolates are not identical. Anti-x sera did not precipitate products from radiolabeled cat lymphoid tumor cells (FL74) and therefore lacked reactivity to the feline leukemia virus-induced tumor-specific antigen, FOCMA.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002415:Cats; D002472:Cell Transformation, Viral; D015683:Gene Products, gag; D005814:Genes, Viral; D006378:Helper Viruses; D007122:Immunoelectrophoresis; D051381:Rats; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics*; D005253:Sarcoma Viruses, Feline / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral; D015683:Gene Products, gag; D014764:Viral Proteins