Variables that may influence the alkaline sedimentation pattern of herpes simplex virus DNA.
Herpes simplex virus (HSV) DNA sediments homogeneously on a neutral sucrose gradient but heterogeneously on an alkaline sucrose gradient. Several factors that may influence the alkaline sedimentation pattern of HSV DNA were examined: e.g., the host cell, cell density at time of infection, multiplicity of infection, and the starting material for HSV DNA purification (whether HSV-infected cells or cell-free virus). Based on alkaline sedimentation analysis, these factors appear to play little or no role in the amount of intact single-stranded HSV DNA observed.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002499:Centrifugation, Density Gradient; D002522:Chlorocebus aethiops; D004277:DNA, Single-Stranded / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D018139:Simplexvirus / Q000032:analysis*; D013395:Sucrose; D014776:Virus Cultivation; D014779:Virus Replication
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D013395:Sucrose