Enzyme formation during solid-substrate fermentation in rotating vessels.
Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-15 rpm), and weight of solids (150 and 300 g) were varied. At low air flow rates (0.05 liter/kg solids/min), alpha-galactosidase production was minimal independent of the rotation rate. At 0.15 rpm and 0.2 liter/kg solids/min air flow rate, invertase production ceased after five days; whereas alpha-galactosidase production continued. The modified Rollacell can be a useful apparatus for studying solid-substrate cultures.
D000388:Air; D001230:Aspergillus / Q000201:enzymology; D005285:Fermentation; D005696:Galactosidases / Q000096:biosynthesis*; D012399:Rotation; D013393:Sucrase / Q000096:biosynthesis*; D000519:alpha-Galactosidase / Q000096:biosynthesis*
D005696:Galactosidases; D000519:alpha-Galactosidase; D013393:Sucrase