Adeno-associated virus replication. The effect of L-canavanine or a helper virus mutation on accumulation of viral capsids and progeny single-stranded DNA.
We have studied the relationship between adeno-associated virus (AAV) DNA replication and virus particle assembly. Formation of empty or full particles and accumulation of AAV capsid proteins was prevented in the presence of the arginine analogue, L-canavanine, or when a temperature-sensitive helper adenovirus was used at the nonpermissive temperature. In each case there was a concomitant inhibition of AAV single-stranded (progeny) DNA accumulation but little or no effect upon synthesis of AAV duplex, replicating form DNA. These results indicate that AAV protein, perhaps in the form of assembled capsids, is required for AAV single-stranded progeny DNA accumulation.
D002172:Canavanine / Q000494:pharmacology*; D002213:Capsid / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000096:biosynthesis*; D000229:Dependovirus / Q000378:metabolism*; D006378:Helper Viruses / Q000378:metabolism*; D008970:Molecular Weight; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects
D004277:DNA, Single-Stranded; D014764:Viral Proteins; D002172:Canavanine