Electron microscopy of phospholipid vesicles reconstituted with purified renal Na,K-ATPase.
Purified Na,K-ATPase after reconstitution into phospholipid vesicles catalyzed an active coupled transport with a ratio close to 3Na/2K. A uniform population of closed vesicles with average diameters close to 900 A are observed after freeze-fracture and thin sectioning. After freeze-fracture intramembranous particles with diameters of 80-100 A are observed. The data suggest that these particles correspond to Na,K-ATPase molecules.
D000818:Animals; D001693:Biological Transport, Active; D005614:Freeze Fracturing; D007668:Kidney / Q000201:enzymology; D008081:Liposomes; D008854:Microscopy, Electron; D010743:Phospholipids; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013552:Swine
D008081:Liposomes; D010743:Phospholipids; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium