title: Effects of a viral laryngotracheitis on the epithelial barrier of chicken airways.
abstract: The effects of a virus infection on the barrier function of tracheal epithelium were compared to the effects of a chemical agent (methacholine) which selectively increases membrane permeability, and both were compared to controls. The disruption of the airway epithelium induced by the virus infection caused an increased permeation of horseradish peroxidase (HRP) through this barrier. Methacholine enhanced HRP uptake from the airway lumen to the blood as compared to controls. Visualization of HRP in the tracheal epithelium by transmission electron microscopy correlated with the radioimmunoassay measurements in the blood. Serial anti-HRP antibody titers were measured by a competitive binding technique. The antigen permeation induced by methacholine was associated with an enhanced anti-HRP antibody production. The larger increase in antigen permeation seen with the viral infection was associated with depressed anti-HRP titers. It was concluded that viral disruption of the airway epithelial barrier may contribute to an increased uptake of orally inhaled antigens. The relationship, however, between the increased antigen penetration consequent to the viral infection and the development of allergy remains unclear.
mesh_terms: D000818:Animals; D002645:Chickens; D004848:Epithelium / Q000502:physiology; D006564:Herpesviridae; D006566:Herpesviridae Infections / Q000503:physiopathology*; D001352:Herpesvirus 1, Gallid; D006735:Horseradish Peroxidase; D008688:Methacholine Compounds / Q000494:pharmacology; D010539:Permeability; D012137:Respiratory System / Q000503:physiopathology*; D012141:Respiratory Tract Infections / Q000503:physiopathology*
chemical_list: D008688:Methacholine Compounds; D006735:Horseradish Peroxidase