Reaction of bovine pancreatic ribonuclease A with 6-chloropurine riboside 5'-monophosphate. Nuclear magnetic resonance studies of the corresponding S-peptide.
The n.m.r. spectra of native S-peptide and of S-peptide II, a derivative obtained after reaction of bovine pancreatic ribonuclease A with 6-chloropurine riboside 5'-monophosphate, both in D2O and in urea-d4, were obtained with a 270 MHz Fourier transform spectrometer. From these spectra it was possible to assign most of the proton resonances of the peptide and the position of the labelling group, the alpha-NH2 of Lys-1, was also deduced.
D000818:Animals; D002417:Cattle; D003903:Deuterium; D004720:Endonucleases; D005583:Fourier Analysis; D007291:Inosine Monophosphate / Q000031:analogs & derivatives*; D009682:Magnetic Resonance Spectroscopy; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments; D011485:Protein Binding; D011487:Protein Conformation; D011685:Purine Nucleotides; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases
D010446:Peptide Fragments; D011685:Purine Nucleotides; C023560:ribonuclease S-peptide; D007291:Inosine Monophosphate; C018488:6-chloropurine 9-beta-D-ribofuranosyl 5'-monophosphate; D003903:Deuterium; D004720:Endonucleases; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic