[Meiotic maturation of the ovocyte of Xenopus laevis : a 4-step mechanism].
Microinjection of the pure inhibitor (1) of cAMP dependent protein kinases (1.5 muM) into Xenopus oocytes triggered 100% germinal vesicle break down (GVBD), faster than progesterone and slower than the maturation promoting factor (MPF). Cholera toxin does not block (I) induced maturation; however cycloheximide inhibits (I) but not MPF induced GVBD.
D000818:Animals; D000242:Cyclic AMP; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D016200:Maturation-Promoting Factor; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology*; D010063:Ovum / Q000166:cytology*; D011374:Progesterone / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D014982:Xenopus laevis
D006133:Growth Substances; D047428:Protein Kinase Inhibitors; D011374:Progesterone; D003513:Cycloheximide; D000242:Cyclic AMP; D011494:Protein Kinases; D016200:Maturation-Promoting Factor
La maturation méiotique de l'ovocyte de Xenopus laevis : un mécanisme en quatre étapes.