Preparation and specificity of antibodies directed toward the ribose methylated nucleotide, 2'-O-methylguanosine 5'-monophosphate.
Antibodies have been produced that recognize the ribosome methylated nucleotide, 2'-O-methylguanosine 5'-monophosphate, pGm. Specificity was tested in a radioimmunoassay. Other ribose methylated nucleotides, deoxyguanosine, and guanosine 5'-monophosphate exhibited negligible cross-reactivity. Elements of antibody recognition, in descending order of importance, were the parent base, the methylated ribose moiety, and the phosphate group.
D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives; D011863:Radioimmunoassay
D000936:Antigen-Antibody Complex; C032212:2'-O-methylguanosine 5'-monophosphate; D006157:Guanosine Monophosphate