Partial purification and characterization of a gelatin-specific protease from the culture media of human pulmonary alveolar macrophages.
A gelatin-specific protease from the culture media of human pulmonary alveolar macrophages has been partial purified by gel filtration and characterized. The macrophages were obtained by bronchopulmonary lavage from the lungs of disease-free smoking volunteers. The gelatin-specific protease initially requires trypsin activation. After chromatographing the culture media on a Sephadex G-200 column, trypsin is no longer required for activation. The gelatin-specific protease reported here shares many properties of previously reported gelatinases. It is inhibited by EDTA, cysteine, dithiothreitol and serum. It is unaffected by other protease inhibitors: phenylmethylsulfonyl fluoride, tosyllysine chloromethyl ketone and p-chloromercuribenzoate. Of all substrates tested activity was observed only with gelatin. It was inactive toward collagen, elastin and methemoglobin. This enzyme may have a role in the digestion of collagen which has been cleaved by a mammalian collagenase.
D000818:Animals; D002417:Cattle; D003470:Culture Media; D010450:Endopeptidases / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism*; D018093:Gelatinases; D006801:Humans; D008264:Macrophages / Q000201:enzymology*; D008666:Metalloendopeptidases; D010196:Pancreatic Elastase / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D013379:Substrate Specificity; D007507:Therapeutic Irrigation
D003470:Culture Media; D005780:Gelatin; D010450:Endopeptidases; D010447:Peptide Hydrolases; D007703:Peptidyl-Dipeptidase A; D010196:Pancreatic Elastase; D010434:Pepsin A; D018093:Gelatinases; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase