the stability in various detergents of transferrin-transferrin receptor complexes from reticulocyte plasma membranes.
Transferrin-membrane protein complexes were solubilized either with 0.4% sodium dodecyl sulfate (SDS), 1% Triton X-100 or 0.5% sulfobetaine 3-14 from the plasma membranes of rabbit reticulocytes previously labeled with 125I and then incubated with 131-labeled transferrin. When the solubilized membranes were analyzed by gel filtration fractionation, marked variation in the preservation of transferrin-transferrin receptor interaction was noted between the three detergents. After SDS solubilization, more than 80% of the 131I-labeled transferrin remained associated with membrane proteins with apparent molecular weight of the transferrin-receptor complexes of 1400 000 and 240 000. In contrast, after Triton X-100 solubilization only 40% of the transferrin was still complexed to membrane proteins with an apparent molecular weight of the complex of 450 000. Dissociation of transferrin from its receptor was most marked following sulfobetaine solubilization, with less than 30% of the transferrin still complexed. Following gel filtration 131I-labeled transferrin-125I-labeled membrane protein complexes were immunoprecipitated with goat specific anti-rabbit transferrin antibodies. The immunoprecipitates were analyzed under stringent dissociating conditions by two SDS-polyacrylamide gel electrophoretic techniques. In a linear 5-25% polyacrylamide gradient the 125I-labeled receptor obtained after membrane solubilization with all three detergents had an apparent molecular weight of 80 000. In contrast, in a different system using 10% polyacrylamide gel two 125I-labeled receptor components were detected wih apparent molecular weights of 90 000 and 80 000. These results demonstrate that estimates of the molecular weight of the transferrin receptor depended on the conditions of electrophoresis and suggest that the transferrin receptor is partially modified, perhaps by glycosylation.
D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives; D002462:Cell Membrane / Q000737:chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003902:Detergents; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D011990:Receptors, Transferrin; D012156:Reticulocytes / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013501:Surface-Active Agents; D014168:Transferrin / Q000378:metabolism*
D003902:Detergents; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D013501:Surface-Active Agents; D014168:Transferrin; D012967:Sodium Dodecyl Sulfate; D001622:Betaine; D011092:Polyethylene Glycols; D017830:Octoxynol