Aldosterone in vitro restores nephron Na-K-ATPase of distal segments from adrenalectomized rabbits.
Aldosterone, when added in vitro to defined nephron segments, dissected from adrenalectomized rabbits, restores the depressed Na+-K+-ATPase activity in the thick ascending limb of the loop of Henle and in the cortical collecting tubule within 1 h of incubation. The direct effect is specific and, as demonstrated for the cortical collecting tubule, dose-dependent. The time course of aldosterone-mediated Na+-K+-ATPase activation is compatible with the conjecture of changes in the enzyme lipid environment.
D000315:Adrenalectomy; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D007684:Kidney Tubules / Q000201:enzymology*; D007685:Kidney Tubules, Collecting / Q000201:enzymology; D007686:Kidney Tubules, Distal / Q000201:enzymology*; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000450:Aldosterone; D000254:Sodium-Potassium-Exchanging ATPase