Determination of adenosine kinase activity by high-pressure liquid chromatography.
High-pressure liquid chromatography (reverse-phase mode) was used to assay adenosine kinase in cell and tissue extracts. The method is optimized for a rapid and selective analysis using 6-methylthiopurine riboside as substrate, isocratic elution and detection at 300 nm. A complete separation of substrate and product is achieved in about 3 min with no interference by other UV-absorbing compounds; the limit of detection is 20 pmoles.
D000248:Adenosine Kinase / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008778:Methylthioinosine / Q000378:metabolism; D008832:Microchemistry / Q000379:methods; D010770:Phosphotransferases / Q000032:analysis*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D008778:Methylthioinosine; D010770:Phosphotransferases; D000248:Adenosine Kinase