A combined assay of three lysosomal marker enzymes: acid phosphatase, beta-D-glucuronidase, and beta-N-acetyl-D-hexosaminidase.
A simplified and rapid method for simultaneous activity measurements of three lysosomal marker enzymes, acid phosphatase, beta-glucuronidase, and beta-N-acetyl-D-hexosaminidase is described. The incubation is carried out in a single test tube and stopped by adding an alkaline sodium dodecyl sulfate solution, thus avoiding centrifugations and allowing for higher Triton X-100 concentrations in the incubation media. Two products of the beta-glycosidases (phenolphthalein and 2-nitrophenolate) are measured spectrophotometrically at the respective wavelengths (555 and 420 nm), and one of the acid phosphatase products is quantitatively determined by measuring inorganic phosphate.
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D005966:Glucuronidase / Q000032:analysis*; D006596:Hexosaminidases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D001619:beta-N-Acetylhexosaminidases
D000135:Acid Phosphatase; D006596:Hexosaminidases; D005966:Glucuronidase; D001619:beta-N-Acetylhexosaminidases