Formation of a lambda (Tn10) tyrR+ specialized transducing bacteriophage from Escherichia coli K-12.
The transposon Tn10, coding for resistance to tetracycline, was inserted close to the tyrR+ gene at min 28 on the Escherichia coli chromosome. The homology between this transposon and a lambda (Tn10) phage was employed to direct integration of lambda close to tyrR+ with subsequent isolation of a lambda (Tn10) tyrR+ transducing phage. Results of restriction endonuclease analysis of the transducing phage are presented.
D010582:Bacteriophage lambda / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics*; D005809:Genes, Regulator; D005814:Genes, Viral; D013752:Tetracycline / Q000494:pharmacology; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D013752:Tetracycline