title: The effects of alpha- and beta-neurotoxins from the venoms of various snakes on transmission in autonomic ganglia.
abstract: We have previously shown that certain commercially available lots of alpha-bungarotoxin block transmission in ciliary and choroid neurons of both pigeon and chicken ciliary ganglia at a concentration of 10 microgram/ml (1.2 microM). The blockade is antagonized by pre-incubation with 100 microM tubocurarine. Further evidence that this blockade is produced by a postsynaptic action, as one would expect of an alpha-neurotoxin, are our findings that: (a) exposure to the toxin prevents the depolarization of ganglion cells normally seen in response to the cholinergic agonist, carbachol; and (b) the blocking activity of the toxin is removed by treatment with membranes purified from Torpedo electric organ containing an excess of alpha-neurotoxin binding sites. A high affinity binding site for [125I]alpha-bungarotoxin was characterized in the chicken ciliary ganglion. However, since it is labelled equally well by lots of alpha-bungarotoxin which block transmission and those that do not, this site does not appear to be involved in the blockade of transmission. alpha-Cobratoxin (from Naja naja siamensis), the alpha-neurotoxin L.s. III (from Laticauda semifasciata) and certain lots of alpha-bungarotoxin produce a partial blockade of transmission in ciliary neurons of the pigeon ciliary ganglion at a concentration of 10 microgram/ml (1.2 microM), but have no effect on transmission in choroid neurons. Two other alpha-neurotoxins from Laticauda semifasciata, erabutoxin a and erabutoxin b, have no effect on transmission in either cell population at this concentration. None of the alpha-neurotoxins tested had any effect on transmission in either the rat superior cervical ganglion or the rat pelvic ganglion at concentrations up to 100 microgram/ml (12 microM). Collagenase treatment of these ganglia, in an attempt to increase access of the toxins to ganglion cells, did not alter these negative results. beta-Bungarotoxin (0.5 microgram/ml, 0.02 microM) produces a complex blockade of transmission in both avian ciliary ganglia and rat superior cervical ganglia. Unlike the action of alpha-bungarotoxin, the blockade of ciliary ganglion transmission by beta-bungarotoxin is irreversible and is not prevented by pretreatment with tubocurarine.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002038:Bungarotoxins / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens; D005725:Ganglia, Autonomic / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009498:Neurotoxins / Q000494:pharmacology*; D051381:Rats; D012910:Snake Venoms / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D002038:Bungarotoxins; D009498:Neurotoxins; D012910:Snake Venoms