Localization of thymulin (FTS-Zn) in mouse thymus. Comparative data using monoclonal antibodies following different plastic embedding procedures.
The distribution of thymulin (FTS-Zn) was studied in thymuses from normal mice (OF1) or autoimmune mice (NZB). Thymulin localization was investigated using immunocytochemical techniques on sections of GMA and epon-embedded mouse thymuses. Two monoclonal antibodies were used: anti-synthetic thymulin and anti-intracellular thymulin. In the immunofluorescence assay, GMA sections allowed a more subtle localization of thymulin in the cytoplasm of epithelial cells (with a vacuolar pattern) compared to the epon sections (with a homogeneous fluorescence in the cytoplasm). In both cases, the number of labeled cells was greater in the medullary region than in the cortex of the thymus. At the electron microscopic level, immunolabeling of epon ultrathin sections showed ferritin distributed in some of the epithelial cell vacuoles. The two monoclonal antibodies revealed similar distributions of thymulin in the thymus. The results obtained in this study confirm that the amount of thymulin is greater in the epithelial cells of normal compared to autoimmune thymuses.
D000818:Animals; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D051379:Mice; D008814:Mice, Inbred NZB; D008854:Microscopy, Electron; D013935:Thymic Factor, Circulating / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism*; D013951:Thymus Hormones / Q000276:immunology / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
D000911:Antibodies, Monoclonal; D013951:Thymus Hormones; D013935:Thymic Factor, Circulating