Integration pattern of hepatitis B virus DNA sequences in human hepatoma cell lines.
Four human hepatoma cell lines established from primary hepatocellular carcinomas were examined for the presence of hepatitis B virus DNA sequences. Reassociation kinetic analysis indicated that the cell lines HEp-3B 217, HEp-3B 14, HEp-3B F1, and PLC/PRF/5 contained two, one, one, and four genome equivalents per cell, respectively. Southern blot hybridization analysis demonstrated that hepatitis B virus DNA was integrated into the cellular DNAs of these cell lines. Further liquid hybridization studies with 32P-labeled HincII restriction fragments of hepatitis B virus DNA established that DNA sequences from all regions of the HBV genome were represented in the integrated viral sequences. Although the three HEp-3B cell lines were derived from the same tumor, they differed significantly in their patterns of integration of hepatitis B virus DNA, the number of copies of viral DNA per cell, and their ability to produce the virus-coded surface antigen.
D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D011995:Recombination, Genetic
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens