[Dependence of ADP-ribosylation of histones of chicken liver nuclei on the intranuclear content of NAD].
The dependence of ADP-ribosylation of chicken liver nuclear histones on NAD concentration in the nuclei was studied under conditions of stimulation of coenzyme synthesis by the nicotinamide and nicotinic acid as well as upon addition of various concentrations of the [Ado-U-14C]NAD nuclei to the incubation mixture. In the first case, the rate of [Ado-U-14C]NAD incorporation into the histones was decreased due to the dilution of the label by the de novo synthesized NAD. The amount of the latter formed under effects of nicotinic acid and nicotinamide increased, correspondingly, from 2,2 X 10(-5) mmol up to 4.1 X 10(-5) and 7.0 X 10(-5) mmol per mg of nuclear protein. The incorporation of [Ado-U-14C]NAD into the histones decreased from 12.0 X 10(-8) mmol after incubation of liver slides with nicotinic acid and nicotinamide down to 8.0 X 10(-8) and 7.0 X 10(-8) mmol, respectively. With a rise in the concentration of exogenous [Ado-U-14C]NAD, the level of ADP-ribosylation of nuclear histones increased, the plot [14C]NAD incorporation at the labeled coenzyme concentration of 25 X 10(-7) mM/mg of histone had a plateau. Changes in the labeled substrate concentration brought about corresponding changes in the average length of the histone-linked poly-(ADP-ribose) chain.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D006657:Histones / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D009243:NAD / Q000096:biosynthesis / Q000378:metabolism*; D009536:Niacinamide / Q000494:pharmacology*; D009539:Nicotinic Acids / Q000494:pharmacology*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*
D006657:Histones; D009539:Nicotinic Acids; D009702:Nucleoside Diphosphate Sugars; D009243:NAD; D009536:Niacinamide; D011064:Poly Adenosine Diphosphate Ribose
Zavisimost' ADP-ribozilirovaniia gistonov iader pecheni tsypliat ot vnutriiadernogo soderzhaniia NAD.