Occurrence of ribitol-containing lipoteichoic acid in Staphylococcus aureus H and its glycosylation.
A ribitol-containing lipoteichoic acid was obtained from the 20,000 x g supernatant fraction of Staphylococcus aureus H by extraction with Triton X-100 followed by fractionation on Sepharose 6B and DEAE-cellulose columns. The purified lipoteichoic acid was composed of phosphate, glycerol, glucose, glucosamine, ribitol, and fatty acids in a molar ratio of 1 : 0.9 : 0.06 : 0.03 : 0.09 : 0.07. Based on the structural analysis of fragments from alkali and HF hydrolysis, the lipoteichoic acid appears to consist of three moieties, namely a ribitol phosphate oligomer, poly(glycerol phosphate) which has about 30 glycerol phosphate units, and beta-glucosyl-beta-glucosyl(1 leads to 1)diacylglycerol. N-Acetylglucosamine was linked to the ribitol residues. The lipoteichoic acid serves as an acceptor of glycosyl moieties from UDP-glucose and UDP-N-acetylglucosamine in the enzyme reaction catalyzed by the membrane preparation. The rate of enzymatic glycosylation was increased by prior treatment of the lipoteichoic acid with N-acetyl-beta-D-glucosaminidase. The glycosylation seems to occur at the ribitol residues of the lipoteichoic acid.
D000118:Acetylglucosaminidase; D055598:Chemical Phenomena; D002621:Chemistry; D005964:Glucosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008070:Lipopolysaccharides; D010504:Periodic Acid; D010710:Phosphates / Q000032:analysis; D010712:Phosphatidic Acids / Q000032:analysis* / Q000378:metabolism; D012255:Ribitol / Q000032:analysis*; D013211:Staphylococcus aureus / Q000032:analysis*; D013402:Sugar Alcohols / Q000032:analysis*; D013682:Teichoic Acids / Q000032:analysis* / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D008070:Lipopolysaccharides; D010710:Phosphates; D010712:Phosphatidic Acids; D013402:Sugar Alcohols; D013682:Teichoic Acids; D010504:Periodic Acid; D012255:Ribitol; D014537:Uridine Diphosphate N-Acetylglucosamine; C009900:lipoteichoic acid; D005964:Glucosyltransferases; D000118:Acetylglucosaminidase; D014532:Uridine Diphosphate Glucose