title: XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genome.
abstract: Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. In both the XbaI and the BglII patterns, four 0.5 M fragments were identified. The arrangement of the fragments was determined by molecular hybridization techniques, and the terminal fragments were identified by lambda exonuclease digestion. The 0.5 M fragments, of which two were located at the same terminus of the genome, contained repeated sequences: one terminally and one inverted internally. These results were in agreement with the existence of two equimolar subpopulations of the varicella-zoster virus genome, differing in the relative orientation of a short region of unique sequences. This region was bounded by the repeated sequences. From the molecular weights of the submolar fragments, a maximal molecular weight of 5 x 10(6) for the repeated region and a minimal molecular weight of 3.5 x 10(6) for the short unique sequence could be calculated.
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C025580:endodeoxyribonuclease XBAI; C024404:BglII endonuclease; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific