Aminoacyl fucosides as possible biochemical markers at tumorigenic and metastatic potential in herpes simplex virus type 2-transformed rat cells.
Two classes of aminoacyl fucosides termed FL3 and FL4 were studied as possible markers of tumorigenic and metastatic potential in herpes simplex virus type 2 transformed rat cells. In the present study, clonal cell lines of transformed highly tumorigenic and metastatic (t-REF-G-1.1), weakly tumorigenic and nonmetastatic (t-REF-G-2.1), nontumorigenic (t-REF-G-2.0), and secondary nontransformed rat embryo fibroblast cells were labeled with [3H]fucose, and cell extracts were analyzed for ratio of radioactivity incorporated into FL3 and FL4. Results indicated that, in extracts from t-REF-G-2.0 and nontransformed rat embryo fibroblast cells, the ratios of FL4/FL3 were 5.78 and 5.71, respectively. In contrast, t-REF-G-2.1 cells exhibited a FL4/FL3 ratio of 1.45, while t-REF-G-1.1 cells exhibited a FL4/FL3 ratio of 0.74. In subclonal cell lines isolated from TPA-treated and mock-treated t-REF-G-2.1 cells, the FL4/FL3 ratios correlated with the tumorigenic and metastatic potential of these subclones in newborn syngeneic White Buffalo rats. These data suggested that alterations in fucose-labeled components can be used to predict the tumorigenic and metastatic potential of herpes simplex virus type 2-transformed rat cells.
D000617:Aminoglycosides / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002855:Chromatography, Thin Layer; D004198:Disease Susceptibility; D005643:Fucose / Q000032:analysis / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism*; D051381:Rats; D018139:Simplexvirus; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000617:Aminoglycosides; D005643:Fucose; D013755:Tetradecanoylphorbol Acetate