Mapping the topography of DNA wrapped around gyrase by nucleolytic and chemical probing of complexes of unique DNA sequences.
Complexes between DNA gyrase and DNA fragments of unique sequences were used to probe the topography of the DNA with nucleases and dimethyl sulfate. The results indicate that the flanking regions, each 50 bp in size, of a 145--155 bp DNA segment resistant to staphylococcal nuclease contain groups of pancreatic DNAase I-susceptible sites that are spaced 10--11 nucleotides apart. Pairs of adjacent DNAase I-sensitive sites on complementary strands are typically staggered by 2--4 bp. The binding of DNA to gyrase confers no protection against alkylation of the DNA by dimethyl sulfate. These properties of the gyrase-DNA complex are reminiscent of those of the nucleosome, and the common underlying structural feature appears to be wrapping of the DNA around a protein core. The gyrase-DNA complex differs from the nucleosome, however, in that it must possess features necessary for the catalysis of DNA chain breakage and the modulation of the DNA-enzyme interaction by ATP. We present evidence that the breakage and rejoining of the DNA by gyrase occur within a central region of the staphylococcal nuclease-resistant DNA segment. The relation of this observation to the mechanism of DNA supercoiling by gyrase is discussed. Addition of ATP or its beta, gamma-imido analog has essentially no effect on the patterns of susceptibilities to DNAase I, implying that the DNA-enzyme contacts mapped by the nuclease ae little affected by ATP-induced conformational changes.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001665:Binding Sites; D004250:DNA Topoisomerases, Type II; D004278:DNA, Superhelical; D003851:Deoxyribonucleases / Q000378:metabolism; D008745:Methylation; D008837:Micrococcus / Q000201:enzymology; D008970:Molecular Weight; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects
D004278:DNA, Superhelical; D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D004250:DNA Topoisomerases, Type II