title: Effects of pressure and temperature on the reactions of horseradish peroxidase with hydrogen cyanide and hydrogen peroxide.
abstract: Reactions of ferric horseradish peroxidase with hydrogen cyanide and hydrogen peroxide were studied as a function of pressure. Activation volumes are small and differ in sign (delta V = 1.7 +/- 0.5 ml/mol for peroxidase + HCN and -1.5 +/- 0.5 ml/mol for peroxidase + H2O2). The temperature dependence of cyanide binding to horseradish peroxidase was also determined. A comparison is made of relevant parameters for cyanide binding and compound I formation.
mesh_terms: D004789:Enzyme Activation; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006856:Hydrogen Cyanide / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism*; D011312:Pressure; D013696:Temperature; D013816:Thermodynamics
chemical_list: D006856:Hydrogen Cyanide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases