title: DNase II in bull and ram sperm tail and mitochondria.
abstract: Extracts of bull and ram sperm tails were prepared by DTT and CTAB treatment. Such extracts contained deoxyribonuclease II, degrading only native double-stranded DNA at acid pH (3.9--4.5). At least three distinguishable DNase II activities were found in these extracts as judged by their sensitivity to thiol compounds and various anions and cations. The deoxyribonuclease II activity is apparently located in or in association with the mitochondria.
mesh_terms: D000818:Animals; D002417:Cattle; D003851:Deoxyribonucleases / Q000032:analysis*; D004706:Endodeoxyribonucleases; D004720:Endonucleases / Q000032:analysis*; D005407:Flagella / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D012756:Sheep; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C019329:deoxyribonuclease II