The growth requirements of SV40 virus transformed Balb/c-3T3 cells in serum-free monolayer culture.
The growth requirements of SV40 transformed Balb/c-3T3 cells have been studied in the absence of serum. For growth in serum-free medium, the cells require (i) insulin, (ii) transferrin, and (iii) cis-unsaturated fatty acids added in combination with fatty acid free bovine serum albumin. The growth rate, saturation density, and morphology of cells grown in this serum-free medium are the same as those of cells grown in serum supplemented medium. This mixture also supports the growth of SV40 transformed Swiss-3T3 cells and SV40 transformed primary mouse embryo cells, but does not support the growth of untransformed Balb/c-3T3 cells. The addition of fibronectin to this mixture allows routine subculture, repeated passage, and indefinite propagation of SV40 transformed Balb/c-3T3 cells. Cells grown in this medium for a period of two months retain their ability to induce tumors when injected into athymic nude mice.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D005227:Fatty Acids / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013539:Simian virus 40; D013997:Time Factors; D014168:Transferrin / Q000494:pharmacology
D005227:Fatty Acids; D005353:Fibronectins; D007328:Insulin; D014168:Transferrin; D012710:Serum Albumin, Bovine