Potassium-stimulated, calcium-dependent release of [3H]GABA from neuron- and glia-enriched cultures of cells dissociated from rat cerebellum.
The release of [3H]GABA from cultures of cells dissociated from the rat cerebellum was investigated. The culture system contained a population of neurons (stellate and basket cells) which were capable of accumulating [3H]GABA and releasing it in a calcium-dependent manner in response to 50 mM potassium. In addition, the release of [3H]GABA for cultured astrocytes was found to be insensitive to potassium depolarization.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D046508:Culture Techniques; D009457:Neuroglia / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D011188:Potassium; D002118:Calcium