[The C3 activating capacity in human serum--measured as monocyte mediated chemiluminescence].
Phagocytosing monocytes form activated oxygen species which can be detected by the luminol-mediated chemiluminescence (CL). C3b-coated particles yield enhanced CL-signals due to their binding to the monocytes' C3b-receptor. Thus, the zymosan-induced monocyte-CL in a medium containing fresh or heat-inactivated serum provides a means of measuring in sera the capacity to activate C3.
D001681:Biological Assay; D003167:Complement Activation / Q000187:drug effects; D003176:Complement C3 / Q000276:immunology*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D010587:Phagocytosis; D011951:Receptors, Complement / Q000276:immunology; D017463:Receptors, Complement 3b; D015054:Zymosan / Q000494:pharmacology
D003176:Complement C3; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D008165:Luminol; D015054:Zymosan
C3-Aktivierbarkeit in humanem Serum--gemessen als Monozyten-vermittelte Chemilumineszenz.