Histochemical studies of oxidoreductases in rat kidney regenerating after mercuric chloride injury.
The activities of succinic dehydrogenase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase and cytochrome oxidase, representatives of the tricarboxylic acid cycle, pentose phosphate pathway, glycolysis and terminal oxidation, respectively, were investigated after necrosis induced with mercuric chloride. 24 hours after the administration of mercuric chloride, the activities of all four enzymes were decreased in the necrotic proximal tubular segments. By the third day of regeneration the activities had decreased or disappeared in all three portions of the necrotic proximal tubules as compared to the 24-hour situation, but they remained constant in the distal tubules; the activity of G6PDH was rather increased. Between the 5th and 12th days the activities gradually increased in the regenerating proximal tubules, while the G6PDH activity of the distal tubules remained elevated, and by the 3rd to 4th week the activities were the same as those in the control animals. Our histochemical results complement the earlier biochemical results of analyses of tissue homogenates localizing the changes of enzyme activity. The histochemical studies suggest that the non-necrotic parts of the nephron contribute to the supplies of energy and substances to the regenerating cell.
D058186:Acute Kidney Injury / Q000139:chemically induced*; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D007668:Kidney / Q000201:enzymology* / Q000503:physiopathology; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008627:Mercuric Chloride; D008628:Mercury; D008630:Mercury Poisoning; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D012038:Regeneration; D013385:Succinate Dehydrogenase
D008627:Mercuric Chloride; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D008628:Mercury