Differences among subfractions of H1 histone in retention of linear and superhelical DNA on filters.
Four kinds of rabbit thymus H1 histone differ among themselves in their ability to retain DNA on nitrocellulose filters. This is true for linear, or superhelical DNA, but the order of effectiveness of the different H1 histones depends on the physical conformation of the DNA. For linear DNA the binding efficiencies of the H1 histones are: RTL2 = RTL3 greater than RTL4 greater than RTL1. This order of effectiveness parallels the effectiveness of the H1 histones previously found for the condensation of linear DNA as observed by circular dichroism and viscosity. The binding efficiencies of the various histones toward superhelical DNA were: RTL4 greater than RTL3 greater than RTL1 greater than RTL2. The variation in amino acid sequence between different rabbit thymus H1 histones might thus introduce structural variations in nucleohistone fibers and perhaps in chromatin.
D000818:Animals; D004278:DNA, Superhelical; D004279:DNA, Viral; D006657:Histones; D007700:Kinetics; D011485:Protein Binding; D011817:Rabbits; D013539:Simian virus 40; D013950:Thymus Gland
D004278:DNA, Superhelical; D004279:DNA, Viral; D006657:Histones