Evaluation of the parameters of the ELISA procedure for feline type C retroviral antigens: assay of FeLV antigens in chronically infected or nonproducer transformed cells.
The successful application of an improved enzyme-linked immunosorbent assay (ELISA) to the specific detection and quantitation of feline leukemia virus (FeLV) is presented. The methods described can detect FeLV antigens at the picogram/milliliter level using a fluorometric assay for peroxidase-linked antibody. The use of a fluorogenic substrate resulted in at least a 9-fold increase in sensitivity of the peroxidase assay. FeLV antigens could be specifically detected in chronically infected cells as well as in feline sarcoma virus-transformed nonproducer mink cells which only partially express viral proteins.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D005256:Leukemia Virus, Feline / Q000276:immunology* / Q000502:physiology; D008907:Mink; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D007074:Immunoglobulin G; D014764:Viral Proteins