Preliminary results on the use of human diploid fibroblasts for inactivated polio virus vaccine production.
Human diploid fibroblast strains WI-38 and MRC 5 can be routinely grown in Eagles medium to densities between 2x10(5) and 1x10(5) cells/cm2. Polio virus type 1 grew to higher titers in WI-38 than MRC 5; types 2 and 3 grew equally well in both strains. After inactivation with 5 mM formaldehyde at 30 degrees C immunogenicity values were obtained which compared favourably with those obtained from monkey kidney preparations.
D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D005347:Fibroblasts; D005557:Formaldehyde / Q000494:pharmacology; D006801:Humans; D017955:Poliovirus / Q000254:growth & development* / Q000276:immunology; D011054:Poliovirus Vaccine, Inactivated; D014776:Virus Cultivation / Q000379:methods*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003470:Culture Media; D011054:Poliovirus Vaccine, Inactivated; D005557:Formaldehyde