Detection and quantification of type C viral proteins in tissues and sera with an enzyme immunoassay.
The detection and quantification of retroviral proteins in cells, tumors, and sera by an enzyme immunoassay technique (enzyme-linked immunosorbent assay [ELISA]) is described. The ELISA technique has been applied to three viral systems: murine leukemia virus, baboon endogenous virus, and simian sarcoma virus. By detecting 0.1 nf of retroviral antigen, the sensitivity of the ELISA is comparable to that of the competition radioimmunoassay technique.
D000042:Absorption; D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification / Q000592:standards; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010215:Papio; D012190:Retroviridae / Q000276:immunology*; D012513:Sarcoma, Experimental / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D007106:Immune Sera; D014764:Viral Proteins