Characterization and determination of neuropeptides by high-performance liquid chromatography and radioimmunoassay.
A method is described for the separation and analysis of a variety of neuropeptides using reversed-phase high-performance liquid chromatography coupled with radioimmunoassay. The solvent system (an acetonitrile gradient containing 0.08% trifluoroacetic acid) allows UV detection at 206 nm, gives good resolution and, by being volatile, is readily compatible with radioimmunoassay. Three applications of the method are described: (a) thyrotropin releasing hormone immunoreactivity in the rat brain has been characterized; (b) ACTH immunoreactivity in the rat pituitary pars intermedia has been resolved into its component peptides; (c) degradation of luteinizing hormone releasing hormone in vitro has been followed.
D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004723:Endorphins / Q000032:analysis; D004745:Enkephalins / Q000032:analysis; D006728:Hormones / Q000032:analysis*; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D008083:beta-Lipotropin / Q000032:analysis
D004723:Endorphins; D004745:Enkephalins; D006728:Hormones; D010455:Peptides; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D008083:beta-Lipotropin