delta 9-Tetrahydrocannabinol by GLC-MS validated radioimmunoassays of hemolyzed blood or serum.
A direct radioimmunoassay (RIA) of delta 9-tetrahydrocannabinol (delta 9-THC) in unextracted hemolyzed blood or serum is described. The dose-response curve in the range of 5-50 ng/mL (serum or blood) was linear on log-logit transformation and iterative weighted regression. Validation studies included testing for precision, accuracy and antibody specificity as well as confirmation of RIA results (marijuana smoker samples) by gas liquid chromatography-mass spectroscopy (GLC-MS). This routine method will greatly augment cannabinoid investigative programs.
D000818:Animals; D000918:Antibody Specificity; D002186:Cannabinoids / Q000097:blood; D013759:Dronabinol / Q000097:blood* / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006041:Goats / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D002186:Cannabinoids; D013759:Dronabinol