[Channel-forming protein from a Na, K-ATPase preparation from porcine renal medulla].
Protein inducing formation of the monovalent cation selective channels on bilayer lipid membrane (BLM) was isolated by ethanol extraction from the great subunits of Na+, K+ ATPase or pork kidney microsomes. Conductance of the single channels in the presence of 2 microgram/ml of protein, 0.016 A KC1 and 0.08 M NaCl is equal to 40 p. The membrane potential of a ten-fold potassium ion gradient is close to a theoretical one. Ouabain (5 X 10(-5) M-1 X 10(4) M) inhibits the formation of the channels. KCl (0.03-0.08 M) eliminates the ouabain effect.
D000818:Animals; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology*; D007679:Kidney Medulla / Q000201:enzymology*; D010042:Ouabain / Q000378:metabolism / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011485:Protein Binding; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*; D013552:Swine
D007473:Ion Channels; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium
Kanaloobrazuiushchiĭ belok iz preparata Na, K-ATFazy mozgovogo sloia pochek svin'i.