An improved method for the collection of serial cells for electron microscopic analysis of their immunocytochemical stain.
A technique to find serial views of cells stained for more than one hormone is described. As the number of stains employed is increased, there is less probability that matching views for all stains will be found. We have found a set of 6 series (including 2 sections per grid and 3 alternating stains) to be optimal for recognition of morphology and collection of a significant number of cells.
D000324:Adrenocorticotropic Hormone / Q000032:analysis; D003584:Cytological Techniques; D005640:Follicle Stimulating Hormone / Q000032:analysis; D007118:Immunoassay; D007986:Luteinizing Hormone / Q000032:analysis; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000032:analysis / Q000648:ultrastructure
D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone