Primary sequence of two regions of mouse pro-adrenocorticotropin/endorphin.
The amino acid sequences of two previously uncharacterized regions of the mouse anterior pituitary common precursor to adrenocorticotropin (ACTH) and beta-endorphin (pro-ACTH/endorphin) were determined. Portions of the NH2-terminal region of pro-ACTH/endorphin (called the 16K fragment) and the region between ACTH and beta-endorphin (called gamma-lipotropin) were sequenced by Edman degradations of biosynthetically labeled immunoprecipitated proteins and by Edman degradations of purified 16K fragment and beta-lipotropin. With a combination of these two approaches, 29 of the first 34 residues at the NH2-terminal end of the mouse 16K fragment were determined. The NH2-terminal region of the mouse 16K fragment was found to be nearly identical with the homologous porcine and bovine molecules. The complete amino acid sequence of the NH2-terminal region of gamma-lipotropin was determined. In contrast to the highly conserved nature of the 16K fragment, mouse gamma-lipotropin was found to differ substantially from the gamma-lipotropins of other species. Although the NH2-terminal and beta-melanotropin-like regions of the mouse gamma-lipotropin are similar to the corresponding regions of other gamma-lipotropins, the intervening region of mouse gamma-lipotropin is substantially shorter than it is in other gamma-lipotropins. In addition, mouse gamma-lipotropin lacks the pair of basic amino acids that normally mark the proteolytic cleavage site used to produce beta-melanotropin from gamma-lipotropin.
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002460:Cell Line; D004723:Endorphins / Q000096:biosynthesis; D006801:Humans; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D010908:Pituitary Hormones, Anterior / Q000096:biosynthesis; D010911:Pituitary Neoplasms; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors / Q000096:biosynthesis; D011865:Radioisotope Dilution Technique; D013045:Species Specificity; D013462:Sulfur Radioisotopes; D014316:Tritium; D014357:Trypsin
D000596:Amino Acids; D002250:Carbon Radioisotopes; D004723:Endorphins; D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D013462:Sulfur Radioisotopes; D014316:Tritium; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D014357:Trypsin