ELISA for the detection and quantification of C-type viral glycoprotein (gp70) using antibodies that recognize the protein moieties of the glycoproteins.
The enzyme-linked immunosorbent assay (ELISA) technique was applied to the detection and quantification of simian sarcoma-associated virus (SiSV)-glycoprotein gp70 in virus preparations and cells and compared to the RIA competition technique. By detecting 0.1 ng of retroviral glycoprotein, its sensitivity is comparable to that of the RIA technique. It was further shown that the antibodies used recognize the protein and not the sugar components of the glycoprotein.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002143:Callitrichinae; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D051379:Mice; D012367:RNA, Viral / Q000032:analysis*; D011863:Radioimmunoassay; D012190:Retroviridae / Q000032:analysis* / Q000276:immunology; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000032:analysis*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins