Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high-molecular-weight RNA.
A detailed characterization of the genome of an endogenous, ecotropic type C virus, the Akv virus, is presented. Approximately 100 RNase T1-resistant oligonucleotides characteristic of the Akv genome were identified by two-dimensional gel electrophoresis, and the complete nucleotide sequence is presented for 75 of these oligonucleotides. A correspondence between the sequence of some of these oligonucleotides and the amino acid sequence of some virus-coded gag gene proteins is reported. For this study we developed methods suitable for the analysis of high-molecular-weight RNA species in nanogram quantities. The in vitro labeling procedures used here led to uniform labeling of the unique oligonucleotides.
D000406:AKR murine leukemia virus / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000032:analysis*; D008970:Molecular Weight; D009711:Nucleotides / Q000032:analysis; D009841:Oligonucleotides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012190:Retroviridae / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism
D009711:Nucleotides; D009841:Oligonucleotides; D012367:RNA, Viral; D012260:Ribonucleases; D000469:Alkaline Phosphatase