title: Multiple innervation of Purkinje cells by climbing fibers in the cerebellum of the adult staggerer mutant mouse.
abstract: Intracellular recordings from Purkinje cells (PC) in the cerebellum of adult staggerer mutant mice revealed that the orthodromic response of PCs to juxtafastigial (JF) stimulation closely resembled a climbing fiber response (CFR). However, for most of the PCs studied, these responses were graded in a stepwise manner when the stimulus strength was increased. The underlying excitatory synaptic potentials (EPSPs) had the typical shape of EPSPs mediated through climbing fibers (CFs), but their size fluctuated in discrete steps, the highest one reaching the firing level. In the same PCs, the size of the spontaneous EPSPs fluctuated in a similar fashion and the frequency of each step was in the range of CF-mediated EPSPs. These results strongly suggest that in staggerer mice several CFs synapse with each PC instead of a single CF as in normal adults. Furthermore, the activation through some of these CFs does not reach the firing level of the corresponding PC.
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000502:physiology*; D004558:Electric Stimulation; D008564:Membrane Potentials; D051379:Mice; D008818:Mice, Neurologic Mutants; D011689:Purkinje Cells / Q000502:physiology; D011930:Reaction Time / Q000502:physiology; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission