Expression of cloned beta-endorphin gene sequences by Escherichia coli.
DNA coding for the opiate peptide beta-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid beta-galactosidase/beta-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active beta-endorphin.
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D004723:Endorphins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D007763:Lac Operon; D051379:Mice; D010957:Plasmids; D011957:Receptors, Opioid / Q000378:metabolism; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D004723:Endorphins; D011957:Receptors, Opioid; D001616:beta-Galactosidase; D014357:Trypsin