Identification and mapping of a second proline permease Salmonella typhimurium.
In this paper we demonstrate the existence of a second proline permease, gene proP, in Salmonella typhimurium. Uptake assays demonstrate that this second proline permease has 5 to 10% the uptake rate of the putP permease, the cell's major proline permease, when assayed at 20 microM proline. Genetic mapping by Hfr and P22-mediated genetic crosses placed the second proline permease gene at 92 min on the S. typhimurium genetic map, near the genes for melibiose utilization. F'-mediated complementation tests indicated that Escherichia coli also has the proP gene.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D005796:Genes; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins / Q000235:genetics*; D011392:Proline / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D026901:Membrane Transport Proteins; D011392:Proline