[Glucoamylase immobilization on silochrome using gossypol].
The paper is concerned with conditions of glucoamylase binding with silanized silochrome using gossypol, dialdehyde isolated from cotton-plant. Kinetic properties of the immobilized enzyme are studied. The enzyme pH optimum does not change with immobilization and the temperature optimum is shifted from 50 degrees to 60 degrees C; a certain increase of the seeming Km is also observed. A high yield of the enzyme activity in immobilization evidences for the possibility of using gossypol as a binding agent in glucoamylase immobilization.
D002384:Catalysis; D004800:Enzymes, Immobilized; D005782:Gels; D005087:Glucan 1,4-alpha-Glucosidase; D005959:Glucosidases; D006072:Gossypol; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058428:Silica Gel; D012822:Silicon Dioxide; D013696:Temperature
D004800:Enzymes, Immobilized; D005782:Gels; D058428:Silica Gel; D012822:Silicon Dioxide; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase; D006072:Gossypol
Immobilizatsiia gliukoamilazy na silokhrome s ispol'zovaniem gossipola.