Quantitation of proinsulin mRNA sequences in hamster insulinoma cells in culture by molecular hybridization.
The content of proinsulin mRNA sequences was measured in a cultured cell line established from a transplantable hamster islet cell tumor, by hybridization with proinsulin cDNA. The cells cultured in vitro were found to contain a significant amount of proinsulin mRNA sequences, compared with non-insulin-producing hamster tissues, and seem to be a useful system for the study of insulin gene expression.
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D007700:Kinetics; D009374:Neoplasms, Experimental / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011384:Proinsulin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D011384:Proinsulin