Transmitter sensitivity of neurons assayed by autoradiography.
Ionic conductance channels that are opened by activating nicotinic acetylcholine receptors at synapses of sympathetic neurons are permeable to small organic amines. Uptake of a tritium-labeled amine through these channels can be measured by autoradiography. This provides a simple and direct way to assess the sensitivity of individual neurons to acetylcholine without using microelectrodes.
D000109:Acetylcholine / Q000494:pharmacology; D000376:Agmatine; D000818:Animals; D001345:Autoradiography / Q000379:methods; D002463:Cell Membrane Permeability / Q000187:drug effects; D006146:Guanidines; D007473:Ion Channels / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology; D010277:Parasympathomimetics / Q000494:pharmacology*; D011898:Ranidae; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism*
D006146:Guanidines; D007473:Ion Channels; D010277:Parasympathomimetics; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D009538:Nicotine; D000376:Agmatine; D000109:Acetylcholine