Streptococcal extracellular NAD+ nucleosidase. Characterization of changes occurring during purification.
Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving primarily as its source. In its effect on the enzyme this component can be replaced with bovine albumin. It follows from the time relationship of the NAD decomposition that the mechanism of the studied enzymic reaction is not hydrolytic. In addition to nicotinamide, a product of polymeric character is formed.
D000818:Animals; D002417:Cattle; D003470:Culture Media; D006321:Heart; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000302:isolation & purification* / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000201:enzymology*; D013696:Temperature; D014020:Tissue Extracts / Q000494:pharmacology
D003470:Culture Media; D014020:Tissue Extracts; D009243:NAD; D009244:NAD+ Nucleosidase