The association of tetrodotoxin-sensitive, sodium-selective ionophore of brain membranes with liposomes.
The tetrodotoxin-sensitive, sodsium-selective ionophore of nerve membranes has been associated with liposomes by adding the solubilized brain microsomal fraction to a cholate/phospholipid dispersion and subsequently removing the detergent from suspension by using gel chromatography. A stimulation of the efflux of sodium from the vesicles was observed in the presence of veratrine. Tetrodotoxin itself did not effect the sodium permeability, but inhibited the veratrine-induced increment. The activation was absent in the liposomes prepared without soluble membrane proteins. The effects demonstrated for tetrodotoxin and veratrine were specific for the Na+ movement. It was possible to precipitate the tetrodotoxin-sensitive ionophore by use of (NH4)2SO4.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D012979:Sodium Radioisotopes; D013779:Tetrodotoxin / Q000494:pharmacology*; D014702:Veratrine / Q000494:pharmacology
D007473:Ion Channels; D008081:Liposomes; D012979:Sodium Radioisotopes; D013779:Tetrodotoxin; D012964:Sodium; D014702:Veratrine; D012413:Rubidium