Increased superoxide generation by normal granulocytes incubated in sera from patients with psoriasis.
Sera from patients with untreated psoriasis were found to induce increased superoxide anion (O-2) generation when incubated with normal granulocytes (PMNs) and zymosan. Sera from patients receiving systemic chemotherapy induced O-2 generation which was similar to that of normal sera and significantly lower than sera from the untreated patients. O-2 production was measured by superoxide dismutase inhibitable ferricytochrome C reduction and was dependent on the presence of both zymosan and a heat labile serum factor. Serum C3c and C5 levels were elevated in both treated and untreated groups of psoriasis patients while C4 was elevated only in untreated patients. serum ceruloplasmin, a O-2 scavenger, was not decreased in patients with psoriasis, and consequently does not account for the increased O-2 generation. These data suggest that sera from patients with psoriasis have an increased capacity to activate PMNs. Activation of PMNs in cutaneous and joint lesions may play a pathogenic role in psoriasis.
D002570:Ceruloplasmin / Q000032:analysis; D003167:Complement Activation; D003165:Complement System Proteins / Q000032:analysis; D003574:Cytochrome c Group / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000096:biosynthesis*; D011565:Psoriasis / Q000097:blood* / Q000209:etiology; D013481:Superoxides / Q000096:biosynthesis*; D015054:Zymosan / Q000494:pharmacology
D003574:Cytochrome c Group; D013481:Superoxides; D003165:Complement System Proteins; D015054:Zymosan; D002570:Ceruloplasmin; D010100:Oxygen