Cloning the quinic acid (aq) gene cluster from Neurospora crassa: identification of recombinant plasmids containing both qa-2+ and qa-3+.
A 22.2-kb insert of Neurospora crassa DNA containing at least two of the genes from the inducible catabolic quinic acid pathway has been cloned into the cosmid vehicle pHC79 resulting in a recombinant plasmid, pMSK308. The qa-2+ locus (which encodes catabolic dehydroquinase) is functionally expressed in both Escherichia coli and qa-2 mutants of N. crassa transformed with pMSK308 plasmid DNA. Expression of the qa-3 gene (which encodes quinate dehydrogenase) is only detected upon reintroduction into N. crassa. Results were also obtained which suggested that the qa-4 gene, which maps between qa-2 and qa-3, may also be present on both pMSK308 and the previously described plasmid pVK88. Certain anomalies in the types of N. crassa transformants obtained with pMSK308 plasmid DNA were noted.
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000235:genetics*; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D010957:Plasmids; D011801:Quinic Acid / Q000378:metabolism*
D004274:DNA, Recombinant; D011801:Quinic Acid; D000429:Alcohol Oxidoreductases; C022511:quinate dehydrogenase; D004262:DNA Restriction Enzymes; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase