Hydrolysis of Cefotaxime by a beta-lactamase from Bacteroides fragilis.
A beta-lactamase isolated from a strain of Bacteroides fragilis subsp. fragilis possessed hydrolytic activity toward cefotaxime. This antibiotic was degraded to a lower extent than was cephalothin, cephaloridine, and cefamandole, whereas cefoxitin remained unaffected by the enzyme. Kinetic parameters Vmax and Km for cefotaxime were calculated at 0.172 mumol/min and 1.1 X 10(-2) mM, respectively.
D001441:Bacteroides fragilis / Q000201:enzymology*; D002439:Cefotaxime; D002511:Cephalosporins / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000494:pharmacology*
D002511:Cephalosporins; D001618:beta-Lactamases; D002439:Cefotaxime