[Use of immunofluorescence technic for the diagnosis of rotavirus infection in the calf].
A methodical account is given of possible applications of the immuno-fluorescence technique in the diagnosis of rotavirus infection of calf. The technique has proved suitable for routine checks for both its low input in terms of method and hardware and its potential of diagnostic information. The two latter methods are best applicable under routine conditions to testing of faeces-inoculated cell cultures as well as to the detection of rotavirus from faecal smears and frozen intestinal sections. In investigations on the dynamics of rotavirus development in cell cultures, the first freshly formed virus protein was detected six hours from inoculation. The time of one replication cycle was found to be between 16 and 18 hours, with several replication cycles running consecutively, when it comes to virus strains which have become adapted to the cell cultures concerned.
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D046508:Culture Techniques; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique / Q000662:veterinary*; D007422:Intestines / Q000382:microbiology; D007668:Kidney; D012087:Reoviridae / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D012401:Rotavirus / Q000302:isolation & purification*; D014776:Virus Cultivation
Der Einsatz der Immunfluoreszenztechnik für die Diagnostik der Rotavirusinfektion des Kalbes.