Study on the single radial complement fixation (SRC-fix) test.
We examined whether the single radial complement fixation (SRC-fix) test is applicable to serological diagnosis of viruses. The viruses used for the examination were influenza A and herpes simplex viruses. SRC-fix test was shown to be a very simple method, that is, serum samples were added to agarose plate A containing complement and CF antigen, and agarose plate B, containing antibody-coated erythrocytes, was layered on top of the plate A to form zone areas of unlysed cells. CF titres of the samples were determined from the square of the zone diameter of unlysed cells. Thus SRC-fix test is suggested as a new method for diagnosis of viral diseases.
D000914:Antibodies, Viral / Q000032:analysis; D003168:Complement Fixation Tests / Q000379:methods*; D006561:Herpes Simplex / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology
D000914:Antibodies, Viral