Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll).
A method for isolation of eosinophils from human peripheral blood using isosmolar solutions of polyvinylpyrrolidone-coated silica gel (Percoll) is described. The purity ranged from 86 to 99% eosinophils in the final preparation and the recovery was 38-56%. The separation technique did not affect the viability or the metabolic capacities of the cells.
D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D004804:Eosinophils / Q000378:metabolism; D006801:Humans; D010101:Oxygen Consumption; D011205:Povidone; D012822:Silicon Dioxide
D012822:Silicon Dioxide; D011205:Povidone