Purification of phytase from rat intestinal mucosa.
Phytase has been purified from intestinal mucosa of rats fed on a phytic acid-enriched diet. The purification consisted of four steps: homogenate preparation, ultrafiltration procedure, DEAE cellulose chromatography, and Sephadex G-100 chromatography. The enzyme was purified 828-fold, as can be seen from the polyacrylamide gel electrophoresis pattern.
D010832:6-Phytase / Q000302:isolation & purification*; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007413:Intestinal Mucosa / Q000201:enzymology*; D008297:Male; D051381:Rats; D014462:Ultrafiltration
D010832:6-Phytase