Studies on the site in the regulatory subunit of type I cAMP-dependent protein kinase phosphorylated by cGMP-dependent protein kinase.
The amino acid sequence around the site of the regulatory subunit of type I cAMP-dependent protein kinase (RI) that is phosphorylated by cGMP-dependent protein kinase has been determined. This site was found to be located near  the site on RI previously shown to be very sensitive to hydrolysis by trypsin (Potter, R. L., and Taylor, S. S. (1979) J. Biol. Chem. 254, 2413-2418). The primary sequence surrounding the site is as follows: -Lys-Ala-Gly-Ser-Arg-Ala-Asp-Ser-Arg-Glu-Asp-Glu-Ile-Ser-Pro-Pro-Pro-Pro-Asn-Pro-Val-Val-Lys-Gly-Arg-Arg-Arg-Arg-Gly-Ala-Ile-Ser(P)-Ala-Glu-Val-Tyr-Thr-Glu-Glu-Asp-Ala-Ala-Ser-Tyr-Val-Arg-Lys-Val-Ile-Pro-Lys-Asp-Tyr-Lys-Thr-. As described previously (Geahlen, R. L., and Krebs, E. G. (1980) J. Biol. Chem. 255, 1164-1169), this site is specific for cGMP-dependent protein kinase and is not phosphorylated by the catalytic subunit of cAMP-dependent protein kinase.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D004789:Enzyme Activation; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D010748:Phosphopeptides / Q000032:analysis; D011494:Protein Kinases / Q000378:metabolism*
D046911:Macromolecular Substances; D010446:Peptide Fragments; D010748:Phosphopeptides; D000242:Cyclic AMP; D011494:Protein Kinases; D010447:Peptide Hydrolases; D006152:Cyclic GMP