Hormone-sensitive lipase of rat adipose tissue: correlation of activity with a protein of molecular weight 84 000.
Hormone-sensitive lipase of rat adipose tissue was partially purified. The enzyme retained its capacity to be activated by cyclic AMP-dependent protein kinase throughout purification. When the partially purified 32P-labeled preparation was subjected to two-dimensional gel electrophoresis, the enzyme activity was found to be associated with a 32P-labeled protein of molecular weight 84 000. The result suggests that this 32P-labeled protein represents hormone-sensitive lipase or the catalytic subunit of the enzyme.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004586:Electrophoresis; D008970:Molecular Weight; D051381:Rats; D002787:Sterol Esterase / Q000302:isolation & purification*
D000242:Cyclic AMP; D002787:Sterol Esterase