Simultaneous induction of three catabolic enzymes in Escherichia coli.
During a simultaneous induction of three enzymes which are subject to catabolite repression (beta-galactosidase, tryptophanase and amylomaltase, or beta-galactosidase, tryptophanase and D-serine deaminase) in a batch culture, the rates of synthesis of beta-galactosidase and tryptophanase decreases, while the rates of synthesis of amylomaltase and D-serine deaminase remain unaffected. The addition of cAMP brings about a considerable increase of the rate of synthesis of D-serine deaminase and a partial synthesis rate increase of beta-galactosidase whihle the synthesis rate of tryptophanase remains lowered and the synthesis rate of amylomaltase remains unaffected. In a continuous culture beta-galactosidase, tryptophanase and D-serine deaminase are synthesized simultaneously at a maximum rate without mutual influence. The addition of cAMP increases the rate of synthesis of all three enzymes.
D000242:Cyclic AMP / Q000494:pharmacology; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000096:biosynthesis*; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000096:biosynthesis*; D006004:Glycogen Debranching Enzyme System; D007700:Kinetics; D012695:L-Serine Dehydratase / Q000096:biosynthesis*; D008190:Lyases / Q000096:biosynthesis*; D014368:Tryptophanase / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis*
D005936:Glucans; D006004:Glycogen Debranching Enzyme System; D000242:Cyclic AMP; D005964:Glucosyltransferases; C020569:4 alpha-glucanotransferase; D005696:Galactosidases; D001616:beta-Galactosidase; D008190:Lyases; D014368:Tryptophanase; D012695:L-Serine Dehydratase