Freeze-dried erythrocytes for an indirect hemagglutination test for detection of cytomegalovirus antibodies.
An indirect hemagglutination test with lyophilized, fixed, tanned, and cytomegalovirus (CMV)-sensitized sheep erythrocytes for the detection of CMV antibodies is reported. To avoid nonspecific hemagglutination, cells were fixed with glutaraldehyde or Formalin directly in whole blood. The lyophilized, CMV-sensitized erythrocytes obtained by this technique were stable up to 9 months at 37 degrees C and retained the same reactivity at fresh, CMV-sensitized cells. Indirect hemagglutination performed with lyophilized, sensitized cells was highly efficient in detecting CMV-antibodies as compared with complement fixation and enzyme immunoassay.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005612:Freeze Drying; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D012756:Sheep / Q000276:immunology
D000914:Antibodies, Viral