title: Simian virus 40 transcriptional complexes incorporate mercurated nucleotides into RNA in vitro.
abstract: Simian virus 40 Sarkosyl transcriptional complexes incorporated mercurated nucleotide precursors into preinitiated RNA chains. Synthesis with mercurated precursors was three- to fivefold slower than with nonmercurated nucleotides (12 to 20 pmol per 10(6) cells per h at 25 degrees C), and 50 to 70% of the RNA product bound specifically to sulfhydryl-agarose. The amount of mercurated CMP incorporated into RNA was measured by specific binding of [35S]cysteine to the mercury residues. More than 90% of the mercurated RNA hybridized to simian virus 40 DNA.
mesh_terms: D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D002846:Chromatography, Affinity; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D007668:Kidney; D007700:Kinetics; D008628:Mercury; D012367:RNA, Viral / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000378:metabolism; D013539:Simian virus 40 / Q000378:metabolism*; D013347:Subcellular Fractions; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D012265:Ribonucleotides; D003570:Cytidine Triphosphate; D008628:Mercury