Effect of oxidizing agents and sulfhydryl group reagents on beta toxin from Clostridium perfringens type C.
Purified beta toxin from Clostridium perfringens type C was inactivated by the oxidizing agents o-iodosobenzoate (OIBA), oxidized glutathione, and ferricyanide, and by the sulfhydryl group regents 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide, iodoacetamide, and iodoacetic acid, causing loss of activity in various degrees depending on the concentration used. The activity of the toxin was not influenced by exposure to 1.0 mM of p-chloromercuribenzoate. The toxin treated by OIBA or DTNB was reactivated by incubation with 2-mercaptoethanol and dithiothreitol. The data suggest that beta toxin contains thiol groups which are essential for the activity.
D001427:Bacterial Toxins / Q000037:antagonists & inhibitors*; D055598:Chemical Phenomena; D002621:Chemistry; D003016:Clostridium perfringens; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005292:Ferricyanides / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D007463:Iodobenzoates / Q000494:pharmacology*; D008623:Mercaptoethanol / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D001427:Bacterial Toxins; D005292:Ferricyanides; D007463:Iodobenzoates; D013439:Sulfhydryl Reagents; D008623:Mercaptoethanol; D004228:Dithionitrobenzoic Acid; D005978:Glutathione; D005033:Ethylmaleimide; D004229:Dithiothreitol