Modification of platelet function by radical species produced during irradiation of oxygenated water.
Gel-filtered platelets in neutral, oxygen-containing solutions irradiated to a dose of 10 krad with gamma-rays display a significant inhibition of ADP-induced aggregation. Though the superoxide anion radical (O2-.) and H2O2 are generated in water irradiated under these conditions, only catalase, but not superoxide dismutase (SOD), conveyed protection against this inhibition of platelet function. When the platelets are in the presence of 10(-3) M sodium formate, which converts the majority of the radical species formed to O2-., inhibition of aggregation was again observed. Under these circumstances, the addition of catalase significantly decreased this inhibition, whereas the addition of SOD was without effect. In order to separate the platelet-inhibition effects of the radical species generated in the medium from a direct effect of irradiation on platelet components, relatively stable quantities of O2-. were produced in alkaline solutions and reacted with gel-filtered platelets. Again, an inhibition of ADP-induced aggregation was observed. This inhibition was not significantly altered by the addition of SOD, but was abolished by the addition of catalase, either with or without SOD. Our observation would indicate that O2-. is without direct effect on platelet function, but serves as the precursor of H2O2, which can inhibit platelet reactions.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D005609:Free Radicals; D005720:Gamma Rays; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D010100:Oxygen; D010974:Platelet Aggregation / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D014867:Water / Q000528:radiation effects*
D005609:Free Radicals; D014867:Water; D013481:Superoxides; D000244:Adenosine Diphosphate; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen