title: Possible mechanisms of stimulus-induced vacuolation in serous cells of tracheal secretory glands.
abstract: Several factors affecting the stimulus-induced vacuolation in cat tracheal submucosal glands described in a companion paper [Am. J. Physiol. 241 (Cell Physiol. 10): C18-C24, 1981] were examined. Stimulation with predominantly alpha-adrenergic, beta-adrenergic, and cholinergic agonists at various concentrations and in the presence of appropriate blockers showed that alpha-adrenergic or cholinergic stimulation is more effective in inducing vacuolation than beta-adrenergic stimulation. In addition, Ca- or HCO3- deficient incubation medium or low incubation temperature either blocked or significantly decreased the vacuolation response. Stimulation in the presence of the Na-K-ATPase inhibitor ouabain prevented vacuolation. The known roles of these factors in fluid transport are consistent with the possibility that stimulus-induced vacuolation possibly arises in response to disturbances in cytoplasmic fluid, which are induced in the secretory cell when the fluid component of secretion is intensely stimulated. A model is presented that possibly relates the observed responses to intracellular event and suggests the possibility of ion pumps in the apical membrane.
mesh_terms: D008729:Methoxamine / Q000494:pharmacology; D009092:Mucous Membrane / Q000502:physiology*; D009940:Organoids / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D012627:Sebaceous Glands / Q000187:drug effects / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D014132:Trachea / Q000502:physiology*; D014617:Vacuoles / Q000187:drug effects / Q000502:physiology*
chemical_list: D010042:Ouabain; D011433:Propranolol; D000254:Sodium-Potassium-Exchanging ATPase; D008729:Methoxamine; D010646:Phentolamine