Genetic and physical mapping of recF in Escherichia coli K-12.
Two factor transductional crosses place recF at approximately 82 min on the E. coli chromosome; recF is highly cotransducible with dnaA and gyrB (cou). Transductional analysis with a series of lambda tna specialized transducing phages carrying chromosomal DNA from the tnaA region place recF between dnaA and gyrB. This analysis also indicates that a gene lying in the same region and producing an easily detectable protein (estimated MW of 45 kD) is dnaN and not recF.
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D004259:DNA-Directed DNA Polymerase; D004250:DNA Topoisomerases, Type II