title: Detection and quantitation of cannabinoids in biological fluids: specificity and kinetics after smoking.
abstract: A procedure for the quantitation and detection of cannabinoids in biological fluids (plasma and urine) is described. This method is based on isotopic derivatization and double labeling. delta 9-Tetrahydrocannabinol and two of its metabolites (11-hydroxy-delta 9-THC and 8-beta-hydroxy-delta 9-THC) can be detected and measured in plasma or urine. The use of TLC enables specific separation of cannabinoids. The sensitivity of detection (4 ng/mL) is compatible with cannabinoid kinetics. The procedure can be applied to routine measurements and pharmacokinetic studies.
mesh_terms: D000328:Adult; D002186:Cannabinoids / Q000032:analysis* / Q000097:blood / Q000652:urine; D002855:Chromatography, Thin Layer / Q000379:methods; D013759:Dronabinol / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D006801:Humans
chemical_list: D002186:Cannabinoids; C001427:11-hydroxy-delta(9)-tetrahydrocannabinol; D013759:Dronabinol