[Restriction and electron microscopic analyses of deletion derivatives thermosensitive with respect to maintaining plasmid pEG1].
Temperature-independent deletion mutants of temperature-sensitive in self-maintenance plasmid pEG-1, derived from R-factor RP4, are mapped using the restriction endonucleases PstI, SmaI, EcoRI, BamHI and the heteroduplex analysis. The pEG1 derivatives under study are found to have deletions in the area of ampicillin transposon Tn1 and nearby genes. The left and the right ends of these deletions boundaries are localized between 37.5 MD and 7.6 MD in the RP4 map. Thus far, the area of plasmid RP4 (37.5-7.6 MD) with Tn1 and, presumably, inc gene(s) in it does not have any genes needed for stable maintenance of R-factor. A conclusion is made that the gene RP4, which carries the mutation determining thermosensitive character of pEG1 maintenance and of inhibition of the cell growth, is localized between the EcoRI site and transposon Tn1 at a distance less than 1.0 MD from the latter.
D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D008854:Microscopy, Electron; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D011815:R Factors
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes
Restriktsionnyĭ i élektronno-mikroskopicheskiĭ analizy deletsionnykh proizvodnykh termochuvstvitel'noĭ po podderzhaniu plazmidy pEG1.