Persistence of genomes of both herpesvirus of turkeys and Marek's disease virus in a chicken T-lymphoblastoid cell line.
A cell line tentatively designated as MDCC-BO1(T), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (HVT). BO1(T) cells were T lymphoblastoid cells and the more than 95% of them had Marek's disease (MD) tumor-associated surface antigen (MATSA). However, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-HVT and -MD virus (MDV) sera. The virus could be rescued from BO1(T) cells by co-cultivation with chick embryo fibroblasts (CEF). The DNA of the rescued virus was characterized as HVT DNA by its sedimentation profile in a neutral glycerol gradient and its endonuclease Hind III cleavage-pattern. Ultrastructural studies on CEF infected with the rescued virus revealed the presence of HVT-like virions. However, DNA-DNA reassociation kinetics showed that the BO1(T) cells contained a few copies of NVT and also MDV genomes.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002645:Chickens; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000382:microbiology*; D014422:Turkeys
D000956:Antigens, Viral