A method for detecting the coding DNA for a protein of known sequence in a collection of chimeric plasmids constructed with cDNAs.
A rapid screening method for recognising plasmids containing copies of cDNA corresponding to proteins of known amino acid sequences, is described. The method is based on the computer prediction of the possible restriction sites in the cloned DNAs. It was tested on a series of proteins with known coding DNA sequences and a series of plasmid cloned cDNAs made from pure chicken globin mRNA.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism
D004274:DNA, Recombinant; D012333:RNA, Messenger; D005914:Globins; D004262:DNA Restriction Enzymes