title: Cloning of restriction fragments of DNA from staphylococcal bacteriophage phi 11.
abstract: EcoRI fragments of Staphylococcus aureus bacteriophage phi 11 DNA were cloned in vector plasmid pSA2100 in S. aureus. The clones were analyzed in marker rescue experiments with suppressor- and temperature-sensitive mutants of phi 11 to correlate the genetic and physical map. Several mutants could be identified on the physical map, and a clone containing fragment EcoRI-B of phi 11 DNA expressed immunity to phage infection. In addition, it was found that recombinant plasmids containing phi 11 DNA sequences can be transferred by high-frequency transduction after phage phi 11 infection of host cells.
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics; D013204:Staphylococcus Phages / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics; D014161:Transduction, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D012097:Repressor Proteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes