Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells in culture.
1. Pig aortic endothelial and smooth-muscle cells in culture rapidly catabolize exogenous ATP, ADP or AMP. 2. In both cell types catabolism is due to Mg2+-stimulated ectoenzymes. 3. Inhibition and substrate-specificity studies suggest that both cell types possess three distinct ectonucleotidases, namely nucleoside triphosphatase (EC 3.6.1.15), nucleoside diphosphatase (EC 3.6.1.6) and 5'-nucleotidase (EC 3.1.3.5), as well as nucleoside diphosphate kinase (EC 2.7.4.6). 4. These ectonucleotidase systems could be of importance in the regulation of neurotransmission, blood platelet function and vasodilation.
D017766:Acid Anhydride Hydrolases; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002478:Cells, Cultured; D004727:Endothelium / Q000201:enzymology; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase; D009708:Nucleotidases / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
D000227:Adenine Nucleotides; D009711:Nucleotides; D010770:Phosphotransferases; D009701:Nucleoside-Diphosphate Kinase; D009708:Nucleotidases; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; D043583:Nucleoside-Triphosphatase; C023837:nucleoside-diphosphatase