title: A radioimmunoassay for cyclic AMP (cAMP) obtained by acetylation of both unlabeled and labeled (3H-cAMP) ligand, or of unlabeled ligand only.
abstract: A sensitive radioimmunoassay (limit of detection 7 +/- 1 fmol per tube) for cyclic AMP (cAMP) based on acetylation of both 3H-cAMP and unlabeled ligand was developed. Rabbit anti-cAMP antibodies had an apparent Ka for the acetylated ligand of 2 x 10(10) l/mol. When the unlabeled ligand only was acetylated an increased sensitivity was obtained without loss of specificity.
mesh_terms: D000107:Acetylation; D000242:Cyclic AMP / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D014316:Tritium
chemical_list: D014316:Tritium; D000242:Cyclic AMP