Stability of C-banded and C-bandless microchromosomes in clonal sublines of the RVP3 mouse tumor grown serially in vivo.
Karylogic studies were performed on three monocellular clones derived from mouse RVP3 cells, which had been originally transformed with the Prague strain of Rous sarcoma virus. All clones that had originally contained a stable number of microchromosomes, continues to retain them after prolonged passage in vivo. Centromeric heterochromatin was absent in 32% of the microchromosomes as revealed by C-banding technique. The stability of microchromosomes either positive or negative for centromeric heterochromatin is discussed in relation to double-minute chromatin bodies found in early passages of RVP3 tumor cells.
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002999:Clone Cells; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D001357:Sarcoma, Avian / Q000235:genetics*