[Changes in some properties of 3':5'-AMP-dependent protein kinase from rat skeletal muscles during physical exercise].
During 6-week training of rats the activity of isoenzymes I and II of soluble 3':5'-AMP-dependent protein kinase increases by 22 and 33%, respectively. A long-term physical load does not cause any significant changes in the activity of both isoenzymes. The maximal activity of the isoenzymes from skeletal muscles of the control and experimental rats is observed at the same concentrations of 3':5'-AMP and pH of 6,0-6,5. During training and under physical load the apparent Km values for ATP of both isoenzymes of 3':5'-AMP-dependent protein kinase do not change significantly, whereas that of V shows an increase. The apparent Km and V values for the histone increase for isoenzyme I obtained from skeletal muscles of trained rats both at rest and under physical load. In case of isoenzyme II the Km value for the histone decreases, while that of V remains unchanged. The changes in the properties of isoenzymes I and II of 3':5'-AMP-dependent protein kinase from skeletal muscles suggest the participation of the enzyme in adaptation to systematic muscular activity.
D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000201:enzymology*; D005082:Physical Exertion; D006656:Protamine Kinase / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
D007527:Isoenzymes; D000242:Cyclic AMP; D011494:Protein Kinases; D006656:Protamine Kinase
Izmenenie nekotorykh svoĭstv 3':5'-AMP-zavisimykh proteinkinaz skeletnykh myshts krys pri trenirovke fizicheskimi nagruzkami.