title: Daunorubicin reduction mediated by aldehyde and ketone reductases.
abstract: 1. The cancer chemotherapeutic antibiotic, daunorubicin, is metabolized in rabbit liver by two distinct cytoplasmic reductases, with pH optima at 6.0 and 8.5. 2. A comparison of inhibition and activation of these two enzymes provides evidence of biochemical and kinetic differences, and suggests that the enzymes belong to different classes of carbonyl reductases; the pH 8.5 optimum reductase activity is classified as an aldehyde reductase, whereas the pH 6.0 optimum reductase is a ketone reductase.
mesh_terms: D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000818:Animals; D003630:Daunorubicin / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008278:Magnesium Sulfate / Q000494:pharmacology; D011817:Rabbits
chemical_list: D002731:4-Chloromercuribenzenesulfonate; D008278:Magnesium Sulfate; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D003630:Daunorubicin