Improved methods for automatic monitoring of contracting heart cells in culture.
The present communication describes improved methods for isolating and plating beating heart cells from neonatal rats using collagenase and collagen-coated petri dishes. The amplitude and frequency of contraction are continuously and simultaneously measured under well defined conditions and during prolonged periods of time with a highly sensitive and thermostated instrument. Additions, e.g. drugs and toxic agents, are made through an accessory pump system that involves extensive dilution of the added compound with medium; aliquots of medium can be withdrawn for estimation of metabolites. The system described is reliable and relatively inexpensive and allows a more extensive use of isolated heart cells, especially in studies of heart functions where small changes in amplitude and frequency of beating during prolonged periods of time are important.
D000818:Animals; D000831:Animals, Newborn; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D006321:Heart / Q000502:physiology*; D008297:Male; D003012:Microbial Collagenase; D009200:Myocardial Contraction; D051381:Rats
D003012:Microbial Collagenase