Adenosine deaminase activity and superoxide formation during phagocytosis and membrane perturbation of macrophages.
During phagocytosis and membrane perturbation, mouse macrophages generate superoxide in direct proportion to their intracellular adenosine deaminase activity. It is proposed that since adenosine deaminase controls the amount of substrate available to xanthine oxidase, and the latter produces superoxide during turnover of its substrates, the purine salvage pathway is an important contributor to the superoxide requirement of macrophages. It is further proposed that this may be the basis for the mechanism of the association of adenosine deaminase deficiency with immunodeficiency.
D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D007153:Immunologic Deficiency Syndromes / Q000201:enzymology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009700:Nucleoside Deaminases / Q000378:metabolism*; D010100:Oxygen / Q000096:biosynthesis*; D010587:Phagocytosis; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000096:biosynthesis*; D014969:Xanthine Oxidase / Q000378:metabolism
D013481:Superoxides; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D010100:Oxygen