A comparative study on the u.v. resistance of double-stranded and single-stranded encephalomyocarditis virus RNAs: evaluation of the possible contribution of host-mediated repair.
To reveal previously suggested host-mediated repair of u.v.-induced lesions in dsRNA of encephalomyocarditis (EMC) virus, two sets of experiments have been carried out: (i) samples of dsRNA of EMC virus were irradiated with different doses of u.v. light and their infectivity was assayed in krebs II cells, before and after conversion of dsRNA into a ss form: (ii) samples of ssRNA of EMS virus were similarly irradiated and their infectivity was assayed before and after conversion of ssRNA into a ds form. No evidence for a significant host-mediated repair of dsRNA in this virus-cell system has been obtained.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004686:Encephalomyocarditis virus / Q000235:genetics*; D051379:Mice; D012330:RNA, Double-Stranded / Q000378:metabolism / Q000528:radiation effects; D012367:RNA, Viral / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014779:Virus Replication / Q000528:radiation effects
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D002110:Caffeine