[Metabolism of cholesterol in normal hamster fibroblasts and those transformed by the SV 40 virus. Effect of low density lipoproteins].
The amount of cholesterol and the percentage of esterified cholesterol were increased in transformed cells. The cholesterol synthesis from [14C] sodium acetate was reduced and cholesteryl oleate uptake increased by 3 fold in transformed cells. The activity of acyl coenzyme A-cholesterol-acyltransferase, measured in situ was also increased in transformed cells. Studies with 125I-LDL pointed out an increase of binding, and especially of internalization of LDL by transformed cells. Finally, long term culture in a lipoprotein-deficient medium showed that transformed cells exhibited a higher ability (tested by growth rate and cholesterol synthesis) to adapt themselves to lipid depletion.
D000818:Animals; D002472:Cell Transformation, Viral; D002784:Cholesterol / Q000096:biosynthesis / Q000378:metabolism*; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D013539:Simian virus 40; D002785:Sterol O-Acyltransferase / Q000378:metabolism
D008077:Lipoproteins, LDL; D002784:Cholesterol; D002785:Sterol O-Acyltransferase
Métabolisme du cholestérol dans les fibroblastes de hamsters normaux et transformés par le virus SV 40. Effet des lipoprotéines de basse densité.