title: A transmissible chicken tumour associated with reticuloendotheliosis virus infection.
abstract: Histiocytic lymphosarcomas of the intestine, liver, spleen and sciatic nerve were found at necropsy in a 36-week-old laying hen that was culled from a flock of 1800 birds because of emaciation. Type C particles were observed in ultrathin sections of liver and spleen. The serum of the hen contained reticuloendotheliosis virus (REV) antigen, and antibody against REV, but lacked antibodies reactive with Marek's disease virus or subgroups A and B of Rous sarcoma virus. The tumour was transmitted to chickens using a suspension of the initial tumours. These experimental tumours were then transmitted to further chickens, using cultured spleen cells, viable spleen cells that had been stored frozen, and disrupted spleen cells. The tumours, which developed after incubation periods as short as 2 weeks, were histologically similar to those in the original hen. A few chickens also developed feather abnormalities. The chickens with experimentally transmitted tumours developed antibody against REV and REV antigen was demonstrated in cultured cells from these chickens. The chickens failed to develop antibody against Rous sarcoma virus and only 1 of 29 developed antibody against Marek's disease virus.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002645:Chickens; D005260:Female; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000635:transmission / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology / Q000635:transmission*; D001356:Reticuloendotheliosis virus / Q000276:immunology; D014412:Tumor Virus Infections / Q000276:immunology / Q000635:transmission / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral