Lymphocyte specificity to protein antigens. III. Capacity of low responder mice to beef cytochrome c to respond to a peptide fragment of the molecule.
Lymph node cells derived from A.TH or A.TL mice primed with beef cytochrome c show striking patterns of reactivity when assayed in vitro for antigen-induced T cell proliferation. Whereas cells from A.TH mice respond specifically to beef cytochrome c or peptides composed of amino acids 1-65 and 81-104, cells from A.TL mice respond neither to beef cytochrome c nor to peptide 1-65, but proliferate following exposure to either peptide 81-104 or to a cytochrome c hybrid molecule in which the N-terminal peptide of beef (1-65) was substituted by a similar peptide obtained from rabbit cytochrome c. Thus, T cells from mice phenotypically unresponsive to beef cytochrome may, in fact, contain populations of lymphocytes capable of responding to a unique peptide, the response to which is totally inhibited when the same fragment is presented in the sequence of the intact protein.
D000818:Animals; D000941:Antigens; D002417:Cattle; D003574:Cytochrome c Group / Q000276:immunology / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D006822:Hybrid Cells / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D010455:Peptides / Q000276:immunology; D011487:Protein Conformation; D011506:Proteins / Q000276:immunology*
D000941:Antigens; D003574:Cytochrome c Group; D010455:Peptides; D011506:Proteins