An evaluation of prepackaged columns for the separation of carcinoembryonic antigen from perchloric acid: concise communication.
Commerically available Sephadex buffer-exchange columns for the separation of carcinoembryonic antigen (CEA) from perchloric acid were evaluated. Elution patterns of individual columns were found to be variable, but separation of acid from CEA could be accomplished. Repeatability of replicate elutions and agreement with dialysis results were acceptable. Nevertheless, the method is excessively time-consuming and inefficient for processing large numbers of patient samples.
D002021:Buffers; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002850:Chromatography, Gel / Q000295:instrumentation*; D003956:Dialysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010472:Perchlorates / Q000032:analysis*
D002021:Buffers; D002272:Carcinoembryonic Antigen; D010472:Perchlorates