Cloning of rabbit genomic fragments containing the uteroglobin gene.
Hybrid phages containing the uteroglobin gene were isolated from a library of Charon 4A lambda phages carrying fragments of rabbit genome. Two phages which contain overlapping rabbit DNA covering approximately 35 kilobase pairs (kb) on both sides of the uteroglobin gene have been analyzed. One of the phages contains the complete gene. Digestion of rabbit liver DNA by restriction endonucleases and Southern hybridization showed a pattern identical with that obtained with the DNA of recombinant phages suggesting that no rearrangement has taken place during cloning. These experiments also showed the presence of a single gene for uteroglobin in the rabbit genome. Isolation of this gene is of interest for the study of the mechanism of action of progesterone.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D006023:Glycoproteins / Q000235:genetics*; D008099:Liver / Q000032:analysis; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D014598:Uteroglobin / Q000235:genetics*
D006023:Glycoproteins; D004247:DNA; D014598:Uteroglobin; D004262:DNA Restriction Enzymes