Determination of polypeptide amino acid sequences from the carboxyl terminus using angiotensin I converting enzyme.
A method for sequence analysis of polypeptides starting at the carboxyl terminus is described that utilizes degradation of the polypeptide into dipeptides with angiotensin I converting enzyme. Dipeptides were identified by gas chromatography-mass spectroscopy. Dipeptide alignment was achieved by replicate digestion of the polypeptide after modification at the carboxyl terminus either by chemical or enzymatic removal of one residue or by addition of a single residue. The addition reaction involved coupling of L-alpha-aminobutyric acid under conditions described herein which yielded essentially complete conversions. Unlike sequence determination methods that commence from the polypeptide amino terminus, this procedure does not require that a polypeptide have a free amino terminus for successful application. A number of polypeptides with varying chain lengths (up to 49 residues), containing among them most of the common amino acids, have been successfully analyzed in amounts as low as 5 nmol.
D000595:Amino Acid Sequence; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D010455:Peptides / Q000032:analysis*; D007703:Peptidyl-Dipeptidase A
D007202:Indicators and Reagents; D010455:Peptides; D007703:Peptidyl-Dipeptidase A