Unusually infrequent cleavage with several endonucleases and physical map construction of Bacillus subtilis bacteriophage phi 1 DNA.
HaeIII, BalI, StuI, BamHI, SlaI, and EcoRII did not cut the genome of Bacillus subtilis phage phi 1 at all, whereas ThaI, BglII, EcoRI, SalI, and Bsu1247I cut the genome once or twice. The physical map of the phi 1 genome was constructed with the latter restriction endonucleases.
D001412:Bacillus subtilis; D001426:Bacterial Proteins; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral
D001426:Bacterial Proteins; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C024404:BglII endonuclease; C039566:CGCG-specific type II deoxyribonucleases; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases