title: Morphological and functional differentiation of Kirsten murine sarcoma virus-transformed rat adrenocortical cell lines.
abstract: This study was undertaken to examine relationships of the phenotype of malignant cells to target cell properties and to events early in the transformation process. Eighteen transformed lines were obtained by Kirsten murine sarcoma virus infection of cells from adrenal glands of rats ages 4 to 30 weeks, at first or second passages in culture. They were grown either as fibroblastic adrenocortical stem cells or as more differentiated epithelial cells, depending on culture conditions. Of 14 lines examined for their capacity to synthesize corticosteroids, 11 converted [14C]pregnenolone to progesterone, and one converted to deoxycorticosterone. In vivo, seven lines produced tumors resembling pleomorphic carcinomas, six lines grew as sarcomas, four grew as mixed tumors, and one line produced anaplastic tumors. Distinguishing features in culture of the carcinoma-producing lines were early onset and rapid progression of morphological transformation, a noncohesive epithelial cell form in some lines, lack of extracellular matrix, and, possibly, and origin in older animals. In contrast, sarcoma-producing cells were fibroblastic and cohesive, produced extracellular matrix, and transformed morphologically after longer and less well-defined periods in culture. The variation in histopathology was unrelated to the differentiation of the target cells and to the capacity of the transformed cells to synthesize corticosteroids. The results show that adrenocortical cells, transformed by Kirsten murine sarcoma virus after short-term culture, usually retain some functional differentiation and sometimes resemble human adrenocortical carcinomas histologically. The susceptibility of adrenocortical cells to Kirsten murine sarcoma virus raises the possibility that mesodermally derived epithelia in general may be target tissues for C-type sarcoma viruses.
mesh_terms: D000302:Adrenal Cortex / Q000382:microbiology* / Q000473:pathology; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis; D000306:Adrenal Cortex Neoplasms / Q000382:microbiology; D000818:Animals; D002454:Cell Differentiation; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004848:Epithelium / Q000473:pathology; D005110:Extracellular Space; D007708:Kirsten murine sarcoma virus; D008297:Male; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D011284:Pregnenolone / Q000378:metabolism; D051381:Rats; D009053:Sarcoma Viruses, Murine
chemical_list: D000305:Adrenal Cortex Hormones; D011284:Pregnenolone