title: The araIc mutation in Escherichia coli B/r.
abstract: The araIc allele is a cis-acting mutation which has been used to define the araBAD promoter in Escherichia coli B/r. Nineteen araIc mutants were originally isolated by Englesberg and co-workers as Ara+ "revertants" of an araC deletion mutant (Englesberg et al. J. Mol. Biol. 43:281-298, 1969). The mutants constitutively expressed araBAD gene products in the absence of functional araC activator protein. Eight of the araIc mutations have been cloned by in vivo recombination onto pBR322-ara hybrid plasmids. Restriction and DNA sequence analysis of these araIc mutations showed that they result from a single base-pair change located at -35 in the araBAD promoter.
mesh_terms: D019747:Aldose-Ketose Isomerases; D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase