[Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies (author's transl)].
A direct solid-phase radioimmunoassay (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150 000-fold more sensitive than NT without complement. By means of dRIA ADV antibodies could be detected in swine sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV--specific binding capacity to 125I-labelled ADV antigen. The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D009500:Neutralization Tests; D011557:Pseudorabies / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*
D000914:Antibodies, Viral
Direkter Festphasen-Radioimmunoassay zum Nachweis von Aujeszkyvirus-Antikörpern.