title: Effect of levamisole on lysosomal enzyme release from polymorphonuclear leukocytes and intracellular levels of cAMP and cGMP after phagocytosis of monosodium urate crystals.
abstract: In order to study the influence of levamisole on lysosomal enzyme release, human polymorphonuclear (PMN) leukocytes were incubated with 0.5 mg/ml monosodium urate crystals. The release of neutral proteinase, beta glucuronidase, myeloperoxidase and lactic dehydrogenase as cytoplasmatic marker was measured. Preincubation of PMN with various concentrations of levamisole lasting 1 h resulted in a decrease of all enzymes released. Intracellular levels of both cyclic nucleotides--cAMP and cGMP--rose in PMN preincubated with levamisole, in comparison with untreated cells.
mesh_terms: D000242:Cyclic AMP / Q000097:blood; D006152:Cyclic GMP / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007978:Levamisole / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D010587:Phagocytosis / Q000187:drug effects*; D014527:Uric Acid / Q000494:pharmacology*
chemical_list: D014527:Uric Acid; D007978:Levamisole; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase; D006152:Cyclic GMP