Biochemical evidence for a cytoplasmic 1 alpha,25-dihydroxyvitamin d3 receptor-like protein in rat yolk sac.
The yolk sac in rats is an organ of exchanges between the mother and fetus. A vitamin D-dependent calcium-binding protein (CaBP) has been recently described in this organ. This led us to investigate the presence of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) receptor-like proteins in the yolk sac cytosol. For this purpose we have utilized sucrose gradient centrifugation, Scatchard analysis, and DNA-cellulose chromatography. Our results show that cytosol prepared from rat yolk sacs contains a 3.3 S binding protein for 1,25-(OH)2D3. The binding is a highly specific, saturable process with high affinity(2 X 10(-10) M at 25 degrees C). The sterol-protein complex binds to DNA-cellulose. The 1,25-(OH)2D3 binding protein is present in the yolk sac from at least the 15th day until the 21st day of gestation. In contrast, such a binding protein is not found in the amnion, the other component of fetal membranes. The biochemical parameters of the 1,25-(OH)2D3 binding protein in the yolk sac are similar to those of 1,25-(OH)2D3 cytosolic receptors in vitamin D target organs. This strongly suggests that the 3.3 S protein in the yolk sac may function as a specific receptor, indicating that this organ may be a new target organ for vitamin D.
D000818:Animals; D001667:Binding, Competitive; D002117:Calcitriol; D002846:Chromatography, Affinity; D003600:Cytosol / Q000378:metabolism; D004247:DNA; D004100:Dihydroxycholecalciferols / Q000378:metabolism*; D005260:Female; D006887:Hydroxycholecalciferols / Q000378:metabolism*; D007700:Kinetics; D011247:Pregnancy; D051381:Rats; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000302:isolation & purification / Q000378:metabolism*; D015017:Yolk Sac / Q000378:metabolism*
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D004247:DNA; D002117:Calcitriol