Persistent infection of human lymphoid cells with poliovirus and development of temperature-sensitive mutants.
Exposure of several spontaneously transformed human lymphoid cell lines to attenuated and virulent types 1 and 2 poliovirus strains led to an initial sharp increase in virus titer followed by a persistent infection. Virus was present as late as 80 passages after infection. The morphology, cell count and viability of virus- and mock-infected cells were similar. Approximately 1% of the cells scored as infectious centers, and the cultures could be cured by specific antiserum. Virus produced during persistent infections initiated with a virus that readily forms plaques at 40 degrees, Mahoney virus, showed an increase in temperature sensitivity and a decrease in plaque size.
D002455:Cell Division; D002460:Cell Line; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008214:Lymphocytes / Q000382:microbiology*; D009154:Mutation; D017955:Poliovirus / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D013696:Temperature; D010948:Viral Plaque Assay
D007106:Immune Sera