A new solvent system for the separation of neutral glycosphingolipids.
A solvent system and a column for high performance liquid chromatography for the separation of glycosphingolipids without derivatization is described. A column pakced with porous silica gel (latrobeads) and eluted with a mixture of isopropanol-hexane-water with increasing water content and decreasing hexane content was used. Glycosphingolipids with mono- to dodeca- or tetrakaidecasaccharides were separated within 60 min and the separation pattern was highly reproducible. The method was applied for preparative separation of highly complex glycolipids with blood group activity.
D000433:1-Propanol; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D004910:Erythrocyte Membrane / Q000032:analysis; D006028:Glycosphingolipids / Q000302:isolation & purification*; D006586:Hexanes; D006801:Humans; D012822:Silicon Dioxide; D012997:Solvents
D006028:Glycosphingolipids; D006586:Hexanes; D012997:Solvents; D012822:Silicon Dioxide; D000433:1-Propanol