title: Mass spectographic analysis of stimulated release of endogenous amino acids from rat hippocampal slices.
abstract: Amino acids released from in vitro rat hippocampal slices following stimulation of the Schaffer's collateral pathway, were collected by micro-perfusion and analysed by chemical ionization mass spectrometry, with isotope ratiodetermination as the quantitative technique, through the use of stable, isotopically labeled internal standards. Stimulation at 10 pulses/sec resulted in a doubling of the release of aspartic acid over the resting level and about a 60% increase in glutamate release, which is in essential agreement with studies utilizing K+ depolarization-evoked release from slices.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D046508:Culture Techniques; D004292:Dominance, Cerebral / Q000502:physiology; D005971:Glutamates / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D013058:Mass Spectrometry; D009434:Neural Pathways / Q000378:metabolism; D051381:Rats; D009435:Synaptic Transmission
chemical_list: D000596:Amino Acids; D005971:Glutamates; D001224:Aspartic Acid