Solubilization of chromatin by means of DNase I.
The paper describes a technique for obtaining solubilized rat liver chromatin by means of mild cleaving with DNase I. The procedure gives a high yield of macromolecular fragments which can be further fractionated by gel filtration. Separated fractions differ in the rate of incorporation of chromosomal RNA precursors applied in vivo.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000187:drug effects*; D002843:Chromatin / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D004247:DNA / Q000032:analysis; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000494:pharmacology*; D004720:Endonucleases / Q000494:pharmacology*; D008099:Liver / Q000648:ultrastructure*; D051381:Rats; D012995:Solubility
D002843:Chromatin; D004247:DNA; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I