Condensation of activated diguanylates on a poly(C) template.
We have studied the metal-ion catalysis of a number of reactions of the isomers of ImpGpG on a poly(C) template. In the absence of a catalytic metal ion, oligomers at least up to (pG)20 are obtained from the ImpGpG isomers in a 1-methylimidazole buffer. The Pb2+ ion improves the yield of longer oligomers and changes substantially the distribution of linkage isomers. The Pb2+ ion greatly improves the yield of longer oligomers obtained from G and ImpGpG on a poly(C) template. The self-condensation of ImpGpG in a 2, 6-lutidine buffer is much less efficient than in a 1-methylimidazole buffer. The Zn2+ greatly increases the yield of products from the [3'-5']-linked dimer, but fails to catalyze the formation of long oligomers from the [2'-5']-linked dimer. The bonds formed in the Zn2+-catalyzed self-condensation of ImpG3pG on poly(C) are mainly [3'-5']-linked.
D006150:Guanine Nucleotides; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D007536:Isomerism; D007700:Kinetics; D007854:Lead; D011066:Poly C; D011131:Polyribonucleotides; D013698:Templates, Genetic; D015032:Zinc
D006150:Guanine Nucleotides; D007202:Indicators and Reagents; D011131:Polyribonucleotides; C024662:GpGp; D007854:Lead; D011066:Poly C; D006157:Guanosine Monophosphate; D015032:Zinc