Immunospecificity of antisera against nonhistone nuclear proteins of human lymphoid cells grown in culture.
Rabbit antibodies were obtained to nonhistone protein--DNA complexes (dehistonized chromatin) prepared from two human lymphoblastoid cell lines: the Conception line from an American Burkitt lymphoma and NC-37 from a nonmalignant source. Both antisera showed a high degree of specificity for nuclear proteins of their respective cell lines. This specificity was evident in the reactivity of both whole chromatin and dehistonized chromatin using a quantitative micro-complement fixation assay. The results presented here suggest that DNA present in the antigen is necessary for maintaining the structure of the antigenic site.
D000974:Antibodies, Antinuclear; D000912:Antibodies, Neoplasm; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002051:Burkitt Lymphoma / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans
D000974:Antibodies, Antinuclear; D000912:Antibodies, Neoplasm; D000956:Antigens, Viral; D002868:Chromosomal Proteins, Non-Histone; D019309:Epstein-Barr Virus Nuclear Antigens