The effect of ascorbic acid on the interaction of adrenaline and neostigmine on neuromuscular transmission.
Ascorbic acid is used in the laboratory as a stabilizing agent to delay the oxidation of adrenaline solutions. The rat isolated phrenic nerve-diaphragm preparation was used to study the interaction of ascorbic acid, adrenaline and neostigmine on neuromuscular transmission. While ascorbic acid itself did not affect the response of the preparation to phrenic nerve stimulation, it significantly reduced the response of the preparation to neostigmine and the augmentation of this response by adrenaline. The results emphasize the need to consider the consequences of including preservatives or stabilizing agents in drug solutions when quantitative comparisons are made.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D003964:Diaphragm / Q000187:drug effects; D004347:Drug Interactions; D004837:Epinephrine / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009388:Neostigmine / Q000494:pharmacology*; D009469:Neuromuscular Junction / Q000187:drug effects*; D010791:Phrenic Nerve / Q000187:drug effects; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects*
D009388:Neostigmine; D001205:Ascorbic Acid; D004837:Epinephrine