title: Immunochemical studies of adrenocorticotropin using tritium labeled hormone.
abstract: A radioimmunoassay using specifically tritiated corticotropin (3H-ACTH) of high specific radioactivity (46 Ci/mmol) and full biological activity has been developed. The assay is sensitive to ACTH in the range 200 pg-10 ng. 50% displacement of 3H-ACTH is produced by less than a twofold excess of unlabeled hormone. Immunochemical studies with various ACTH analogs showed that the antiserum recognized the intact molecule better than any of other fragments.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D010455:Peptides / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013552:Swine
chemical_list: D010455:Peptides; D000324:Adrenocorticotropic Hormone