title: Organization of kappa light chain genes in germ-line and somatic tissue.
abstract: We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.
mesh_terms: D000483:Alleles; D000818:Animals; D000916:Antibody Diversity; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D009194:Myeloma Proteins / Q000235:genetics*; D010957:Plasmids; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D004274:DNA, Recombinant; D007127:Immunoglobulin Constant Regions; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins; D004262:DNA Restriction Enzymes