[Cytochemical study of granulocyte enzymes in germ-free animals with adenovirus infections].
The activity of some dehydrogenases and hydrolases was studied by cytochemical methods in the peripheral blood neutrophils of germ-free guinea pigs infected with adenoviruses. The gnotobiotic animals were obtained by hysterotomy in an operation isolation room after which they were transferred into manipulation isolation room and infected with human adenovirus type 1. A depression of enzymes of alpha-glycerophosphate shunt and NADP-H2-diaphorase in neutrophils two days after infection and activation of lactate dehydrogenase and acid phosphatase at 4 days were demonstrated. The pattern of changes in the enzymatic status of intact and infected gnotobiotic animals allowed a diagnosis of adenovirus infection in most cases.
D000135:Acid Phosphatase / Q000378:metabolism; D000257:Adenoviridae Infections / Q000175:diagnosis*; D000818:Animals; D000831:Animals, Newborn; D004796:Clinical Enzyme Tests; D005856:Germ-Free Life; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006168:Guinea Pigs; D006651:Histocytochemistry; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology*
D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D009252:NADPH Dehydrogenase; D000135:Acid Phosphatase
Tsitokhimicheskoe izuchenie fermentov granulotsitov u gnotobiotikov pri adenovirusnoĭ infektsii.