A bacterial test in liquid culture for the detection of mutagenic activity of antibacterial compounds: studies with cephalosporine HR 756.
A detailed procedure for the short term evaluation of mutagenic activity in the Salmonella assay in liquid cultures is given for Cephalosporine HR 756. The essential steps of the test are: a) determination of absence of interference of the pKM101 plasmid present in the bacterial tester strains, b) determination of the doses of the antibiotic required to obtain a limited killing, c) introduction of appropriate controls with known mutagenic compounds active in the presence and in the absence of metabolic activation, d) segregation of mutants. Following this procedure we have determined the absence of any mutagenic activity in the liquid assay of Cephalosporine HR 756.
D000818:Animals; D002439:Cefotaxime; D002511:Cephalosporins / Q000494:pharmacology / Q000633:toxicity*; D003470:Culture Media; D066298:In Vitro Techniques; D009152:Mutagenicity Tests / Q000379:methods*; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D002511:Cephalosporins; D003470:Culture Media; D002439:Cefotaxime