Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli.
Double-stranded DNA copies of the single-stranded genomic RNA of foot and mouth disease virus have been cloned into the Escherichia coli plasmid pBR322. A restriction map of the viral genome was established and aligned with the biochemical map of foot and mouth disease virus. The coding sequence for structural protein VP1, the major antigen of the virus, was identified and inserted into a plasmid vector where the expression of this sequence is under control of the phage lambda PL promoter. In an appropriate host the synthesis of antigenic polypeptide can be demonstrated by radioimmunoassay.
D000956:Antigens, Viral / Q000235:genetics*; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D000956:Antigens, Viral; D012333:RNA, Messenger; D014764:Viral Proteins