Cat satellite DNA. Isolation using netropsin with CsCl gradients.
The absence of centromeric bands in the karyotype of Felis catus is confirmed. It is also confirmed that no satellite band is visible in CsCl density gradients. However, a satellite is observed both by recentrifuging the fraction of the DNA that bands at high density in CsCl and by using netropsin to enhance the resolution of a CsCl gradient containing total F. catus DNA. The satellite, about 0.5% of total DNA, was isolated by repeated centrifugation in CsCl alone and in CsCl with netropsin. Netropsin was removed and a pure satellite DNA obtained. The reassociation kinetics (C0t1/2 less than 10(-3) M . s) show that the satellite is of the simple sequence type and hence a candidate for centromeric heterochromatin. Its cytological localisation awaits in situ hybridisation experiments.
D000818:Animals; D002415:Cats / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D002503:Centromere; D004276:DNA, Satellite / Q000302:isolation & purification*; D006146:Guanidines; D007621:Karyotyping; D009429:Netropsin
D004276:DNA, Satellite; D006146:Guanidines; D009429:Netropsin