title: Lack of Thy 1.2 antigen expression on the surfaces of mouse mammary epithelial cells.
abstract: Monoclonal antisera against mouse Thy 1.2 antigen were used to assess the expression of Thy 1.2 antigen on BALB/c and BALB/cfC3H normal mouse mammary gland epithelial cells (MMEC) and on spontaneous and transplanted BALB/cfC3H mammary adenocarcinoma cells. Immunofluorescence (IF) assays with the monoclonal anti-Thy 1.2 sera demonstrated that Thy 1.2 antigen was not expressed on the surface membranes of MMEC (both virus-free BALB/c and virus-infected BALB/cfC3H) and tumor (BALB/cfC3H) mammary epithelial cells. A specific antiserum against MMEC was used to establish the expression of this normal antigen(s) on the surfaces of both MMEC and tumor mammary epithelial cells obtained from virus-free and virus-infected mice. In addition, the expression of the mammary tumor virus envelope glycoprotein gp52 was determined by indirect IF on the surfaces of virus-infected mammary epithelial cells, both MMEC and tumor cells.
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005260:Female; D007519:Isoantigens / Q000032:analysis; D008321:Mammary Glands, Animal / Q000276:immunology*; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D007519:Isoantigens; D014764:Viral Proteins