title: Activation of the Sendai virus fusion protein (f) involves a conformational change with exposure of a new hydrophobic region.
abstract: The F protein of paramyxoviruses is actively involved in the induction of membrane fusion. This fusion may be between viral and cellular membranes, as in the initiation of infection or in virus-induced lysis of erythrocytes, or between the plasma membranes of different cells. The F protein is activated by proteolytic cleavage to yield two disulfide-linked polypeptides (F1 and F2); however, its mechanism of action is not clear. In the present study, the conformations of the inactive, uncleaved precursor of glycoprotein (F0), and the active, cleaved form (F1,2) have been compared. The UV circular dichroism spectra of the two forms of the F protein indicate that cleavage results in a conformational change. Detergent-binding studies by velocity sedimentation analysis of Triton X-100-protein complexes revealed an increase in exposed hydrophobic surface of the protein on cleavage. The inactive F0 bound an estimated 27 molecules of Triton X-100/F polypeptide; these molecules are presumably bound to the hydrophobic region of the glycoprotein that anchors the spike-like protein in the virus membrane and that is common to both forms of F. The active form, F1,2, bound 67 molecules of Triton X-100. This increase in the number of detergent binding sites upon F protein activation indicates the presence of a hydrophobic region that is peculiar to the active form, and that may be of functional significance in the membrane fusion reaction.
mesh_terms: D002499:Centrifugation, Density Gradient; D002942:Circular Dichroism; D008854:Microscopy, Electron; D017830:Octoxynol; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D011487:Protein Conformation; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D010713:Phosphatidylcholines; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol