Immunological and chemical characterization of rat liver cytochrome c oxidase.
Rat liver cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase; EC 1.9.3.1) was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis into 12 different polypeptide chains. Specific antisera against the holoenzyme and against purified subunits IV and VIII were used to characterize the enzyme complex. The antiserum against subunit IV precipitates from sodium dodecyl sulfate-dissociated mitochondria only subunit IV and from Triton X-100-dissolved mitochondria all 12 polypeptide chains, indicating their integral location within the enzyme complex. Different antisera against the holoenzyme only precipitate subunits IV, V and VIb from sodium dodecyl sulfate-dissociated mitochondria, suggesting the location of these subunits on the surface layer of the complex. Subunit VIII is thought to be located within the complex, since a specific antiserum does not precipitate the complex. The amino acid composition of all 12 protein subunits is different, thus excluding their origin from proteolytic degradation. The proteolytic degradation of subunit IV into IV during isolation of the enzyme was corroborated by the very similar amino acid composition of both proteins.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000936:Antigen-Antibody Complex; D003576:Electron Transport Complex IV / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D005779:Immunodiffusion; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008930:Mitochondria, Liver / Q000201:enzymology; D010455:Peptides / Q000302:isolation & purification; D051381:Rats
D000596:Amino Acids; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D046911:Macromolecular Substances; D010455:Peptides; D003576:Electron Transport Complex IV