title: Ultrastructural development and persistence of guinea pig cytomegalovirus in duet cells of guinea pig submaxillary gland.
abstract: Salivary glands from Hartley guinea pigs were experimentally infected with guinea pig cytomegalovirus (GPCMV) and examined by light and electron microscopy at different time intervals. Characteristic intranuclear and intracytoplasmic viral inclusions were observed in duct cells of infected animals. Viral inclusion counts and infectivity titers in the salivary gland reached maximum levels by 3 to 4 weeks after infection; infectivity persisted, though at reduced levels, for at least 30 weeks. Electron microscopic examination of viral inclusions revealed several developmental events including nucleocapsid assembly, envelopment of nucleocapsids at the inner nuclear membrane and their enclosure by a thin vacuolar membrane. While contained within cytoplasmic vacuoles, enveloped virions acquired surface spikes. Cytoplasmic vacuoles containing virions subsequently coalesced and discharged mature virions at the cell surface into the lumen of the salivary gland duct. The data indicate that the ultrastructural development of GPCMV in the guinea pig salivary gland shows many similarities to that of human cytomegalovirus in humans. The salivary gland may provide a primary locus for virus shedding and horizontal transmission of cytomegalovirus.
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism; D002467:Cell Nucleus / Q000382:microbiology; D003587:Cytomegalovirus / Q000254:growth & development* / Q000648:ultrastructure; D003586:Cytomegalovirus Infections / Q000382:microbiology* / Q000473:pathology; D003593:Cytoplasm / Q000382:microbiology; D006168:Guinea Pigs; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D008854:Microscopy, Electron; D013363:Submandibular Gland / Q000382:microbiology* / Q000648:ultrastructure; D014771:Virion / Q000254:growth & development