Physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue.
The physical mapping of six ts mutations of herpes simplex virus type 2 (HSV-2) is presented. The results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 HSV-1/HSV-2 intertypic recombinants. The order of these mutations on the physical map of HSV-2 is unambiguous and correlates almost exactly with the previously published genetic map of Timbury & Calder (1976). One of the mutants studied (HSV-2 ts12) has apparently two distinct conditionally lethal ts mutations, one in the long and the other in the short region of the HSV genome.
D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D005819:Genetic Markers; D009154:Mutation; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development; D013696:Temperature
D004279:DNA, Viral; D005819:Genetic Markers; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific