title: Probable precursors of [Leu]enkephalin and [Met]enkephalin in adrenal medulla: peptides of 3-5 kilodaltons.
abstract: Adrenal chromaffin granules contain at least 10 peptides, ranging in size from 3 to 5 kilodaltons, that yield, upon digestion with trypsin, peptides that show specific binding to opiate receptors. All are distinctly different from beta-endorphin. Two of these peptides have been purified to homogeneity and subjected to chemical analysis. One is apparently a [Met]enkephalin precursor containing two copies of the [Met]enkephalin sequence. The other peptide contains both [Leu]enkephalin and [Met]enkephalin sequences and is presumably a common precursor of the two forms of enkephalin.
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D004723:Endorphins / Q000096:biosynthesis*; D004745:Enkephalins / Q000096:biosynthesis* / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments; D011498:Protein Precursors / Q000378:metabolism*; D011863:Radioimmunoassay; D011869:Radioligand Assay; D011957:Receptors, Opioid / Q000378:metabolism; D014357:Trypsin
chemical_list: D000596:Amino Acids; D004723:Endorphins; D004745:Enkephalins; D010446:Peptide Fragments; D011498:Protein Precursors; D011957:Receptors, Opioid; D014357:Trypsin