[Quantity of exogenous linear DNA absorbed by E. coli cells treated with Ca2+ cations].
The amount of linear chromosomal DNA entering Ca2+-treated Escherichia coli K 12 cells was estimated from the degree of degradation of exogenous DNA by intracellular nucleases. At least 40% of DNA adsorbed by the cells in the nucleaso-resistant form (45 MD/cell) was discovered to be as intracellular. No significant difference was found between single- and double-stranded DNA.
D002118:Calcium / Q000494:pharmacology*; D002384:Catalysis; D002463:Cell Membrane Permeability / Q000187:drug effects; D004269:DNA, Bacterial / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D043211:Exodeoxyribonuclease V; D005092:Exonucleases / Q000378:metabolism; D013104:Spheroplasts; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D003851:Deoxyribonucleases; D005092:Exonucleases; D043211:Exodeoxyribonuclease V; D002118:Calcium
Kolichestvo ĕkzogennoĭ lineĭnoĭ DNK, poglashchaemoĭ kletkami E. coli, obrabotannymi kationami Ca2+.