Comparison of radioimmunoassay for internal protein of bovine leukemia virus with neutralization test employing VSV-BLV pseudotype.
Two methods for the detection of antibodies to the bovine leukemia virus (BLV) in infected animals were compared for their suitability for the early diagnosis of bovine leukemia - pseudotype neutralization test (PNT) employing vesicular stomatitis virus - bovine leukemia virus pseudotypes (VSV-BLV), and radioimmunoassay test (RIA) for major internal viral protein p24 of the BLV. The comparison was made using more than 300 sera from cows of the herds with high incidence of bovine leukemia. In infected animals the presence of antibodies against virus envelope glycoprotein detected by PNT and antibodies against major structural viral protein p24 detected by RIA were found always coincidentally. Both methods were found highly comparable and suitable for early detection of bovine leukemia virus infected animals.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D001909:Leukemia Virus, Bovine / Q000032:analysis* / Q000276:immunology; D009500:Neutralization Tests; D011863:Radioimmunoassay; D012190:Retroviridae / Q000032:analysis*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis*
D000914:Antibodies, Viral; D014764:Viral Proteins