title: Increase of benzodiazepine binding to the membranes isolated in the presence of diazepam.
abstract: The preparation of brain cells in the presence of diazepam leads to a 60-70% increase in benzodiazepine receptor density. Diazepam may promote the dissociation of endogenous inhibitors (modulators) and/or retard the dissociation of benzodiazepin receptors from the membrane to the medium. Routinely used procedures for tissue preparation reveal only part of the benzodiazepine receptors originally present in the tissue.
mesh_terms: D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D003975:Diazepam / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D008297:Male; D051381:Rats; D011955:Receptors, Drug / Q000032:analysis / Q000187:drug effects*; D011963:Receptors, GABA-A
chemical_list: D011955:Receptors, Drug; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D005445:Flunitrazepam; D003975:Diazepam