Rapid solid-phase enzyme immunoassay for antibodies to viruses and other microbes: effects of polyethylene glycol.
A rapid and sensitive solid-phase enzyme immunoassay (EIA) was developed for determination of serum antibodies, including addition of 4% (w/v) polyethylene glycol (PEG) to the diluent buffers. This modification accelerated the antigen-antibody reactions and made it possible to complete the assay within 2 h and to incubate at room temperature only. The enhancing effect of the polymer was particularly prominent in the second solid-phase immune reaction, the interaction between the antigen-bound antibody and the enzyme-labeled anti-immunoglobulin. The PEG-EIA procedure was successfully applied in the assay of antibodies to rubella, influenza A, herpes simplex and cytomegalo viruses, to Mycoplasma pneumoniae and Toxoplasma gondii.
D000906:Antibodies / Q000032:analysis; D000907:Antibodies, Bacterial / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology; D011019:Pneumonia, Mycoplasma / Q000276:immunology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012412:Rubella virus / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology
D000906:Antibodies; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D011092:Polyethylene Glycols