[Rapid method for the determination of choline kinase activity. Choline kinase in marine invertebrates].
A quick sensitive test is suggested to determine choline kinase (EC 2.7.1.32). The method includes incubation of a substrate with enzyme in microvolume and separation of the formed phosphorylcholine from the initial choline by high-performance thin-layer silica gel chromatography. The procedure of determination is simple, reproducible and takes no more than 30 min. The method reveals high activity of choline kinase in some marine invertebrates. Certain features of the enzyme from the intestine of strongylocentrotus intermedius are described.
D000818:Animals; D002797:Choline Kinase / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007448:Invertebrates / Q000201:enzymology*; D008832:Microchemistry; D010770:Phosphotransferases / Q000032:analysis*; D012617:Sea Urchins / Q000201:enzymology*; D012623:Seawater
D010770:Phosphotransferases; D002797:Choline Kinase
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