The liver-protective enzymes against reduced forms of oxygen in phenobarbital-treated rats.
It is well known that phenobarbital treatment increases the production of superoxide anion by liver microsomes. Since the toxicity of superoxide anion and of its product, hydrogen peroxide, is well documented we measured the activities of the liver enzymes which protect the cell against the reduced forms of oxygen and the microsomal content of lipoperoxides in phenobarbital-treated rats. It was found that no lipoperoxides accumulate in the microsomes and that glutathione peroxidase, glutathione reductase activities, and the peroxidative activity of cytochrome P-450 were increased while catalase and superoxide dismutase activities were not modified.
D000818:Animals; D002374:Catalase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010100:Oxygen / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D008054:Lipid Peroxides; D013481:Superoxides; D003577:Cytochrome P-450 Enzyme System; D010544:Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D010100:Oxygen; D010634:Phenobarbital