title: Reversal of radiation-dependent heat sensitization of Clostridium perfringens spores.
abstract: The effect of solute concentration on the sensitization of Clostridium perfringens spores to heat by ionizing radiation was investigated. As we have shown previously, spores of C. perfringens treated with gamma radiation are now sensitive to subsequent heat treatments than are spores that receive no radiation treatment. When gamma-irradiated spores were heated in the presence of increasing concentrations of glycerol or sucrose, the heat sensitivity induced by irradiation was progressively decreased. The magnitude of the increase in heat resistance induced by extracellular solutes was greater in gamma-irradiated spores than in nonirradiated spores. Based on these observations, it is proposed that the induction of heat sensitivity in spores by radiation is related to the loss of osmoregulatory or dehydrating mechanisms in irradiated spores.
mesh_terms: D003016:Clostridium perfringens / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D005720:Gamma Rays; D005990:Glycerol / Q000494:pharmacology*; D006358:Hot Temperature; D009997:Osmotic Pressure; D012996:Solutions; D013171:Spores, Bacterial; D013395:Sucrose / Q000494:pharmacology*
chemical_list: D012996:Solutions; D013395:Sucrose; D005990:Glycerol