Restoration of guanine nucleotide- and fluoride-stimulated activity to an adenylate cyclase-deficient cell line with affinity-purified guanine nucleotide regulatory protein.
A guanine nucleotide-binding protein purified from turkey erythrocytes by affinity chromatography confers both F-- and guanine nucleotide-stimulation of adenylate cyclase to membranes from CYC- cells, a mutant cell line deficient in these responses. Interaction of turkey erythrocyte membranes with beta-adrenergic agonists before affinity chromatography, which is essential for binding of the guanine nucleotide regulatory protein to the affinity matrix, was also required for recovery of F--stimulation restoring activity in the affinity eluate.
D000262:Adenylyl Cyclases / Q000172:deficiency / Q000378:metabolism*; D002352:Carrier Proteins / Q000494:pharmacology*; D002460:Cell Line; D002846:Chromatography, Affinity; D004789:Enzyme Activation / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000201:enzymology; D005459:Fluorides / Q000494:pharmacology*; D019204:GTP-Binding Proteins; D007545:Isoproterenol / Q000494:pharmacology; D008223:Lymphoma / Q000473:pathology; D011956:Receptors, Cell Surface / Q000494:pharmacology*
D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D005459:Fluorides