Purification of human liver fructose-1,6-bisphosphatase.
Human liver fructose-1,6-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) has been purified 1200-fold using a heat treatment step followed by absorption on phosphocellulose at pH 8 and specific elution with buffer containing the substrate (fructose 1,6-bisphosphate) and allosteric effector (AMP). The enzyme is homogeneous in electrophoresis in polyacrylamide gel, in the presence and absence of denaturing agent. It has a molecular weight of 144 000 and is composed of four identical or nearly identical subunits. Fluorescence spectra indicate that the enzyme does not contain tryptophan residues. The pH optimum is 7.5 and the Km is determined as 0.8 microM. The enzyme is inhibited by AMP in cooperative manner with a K0 x 5 of 6 microM.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D006597:Fructose-Bisphosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D014364:Tryptophan / Q000032:analysis
D000249:Adenosine Monophosphate; D014364:Tryptophan; D006597:Fructose-Bisphosphatase