title: Cat satellite DNA. Isolation using netropsin with CsCl gradients.
abstract: The absence of centromeric bands in the karyotype of Felis catus is confirmed. It is also confirmed that no satellite band is visible in CsCl density gradients. However, a satellite is observed both by recentrifuging the fraction of the DNA that bands at high density in CsCl and by using netropsin to enhance the resolution of a CsCl gradient containing total F. catus DNA. The satellite, about 0.5% of total DNA, was isolated by repeated centrifugation in CsCl alone and in CsCl with netropsin. Netropsin was removed and a pure satellite DNA obtained. The reassociation kinetics (C0t1/2 less than 10(-3) M . s) show that the satellite is of the simple sequence type and hence a candidate for centromeric heterochromatin. Its cytological localisation awaits in situ hybridisation experiments.
mesh_terms: D000818:Animals; D002415:Cats / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D002503:Centromere; D004276:DNA, Satellite / Q000302:isolation & purification*; D006146:Guanidines; D007621:Karyotyping; D009429:Netropsin
chemical_list: D004276:DNA, Satellite; D006146:Guanidines; D009429:Netropsin