Single-strand DNA binding protein from rat liver: interactions with supercoiled DNA.
As shown by competition experiments, the single-strand DNA binding protein from normal rat liver (S25) interacts preferentially with supercoiled DNA compared to relaxed DNA duplexes. When followed both by sedimentation analysis and by nitrocellulose filter assay, the binding of S25 to SV40 supercoiled DNA (FI) appears to be non-cooperative. Saturation is reached at a protein to DNA weight ratio of about 2. The S25-DNA complexes prefixed with glutaraldehyde appear as beaded structures having an average of 14 to 16 beads per SV40 DNA molecules. Cross-linking of S25 bound to SV40 DNA by dimethyl suberimidate allows to detect oligomeric structures containing a maximum of twenty monomers of S25. When complexes are treated by glutaraldehyde, 10% of the genome become resistant against micrococcal nuclease. Moreover, S25 affects the DNA helical structure. Superhelical forms are generated by the association of S25 with SV40 DNA, in the presence of nicking-closing enzyme.
D000818:Animals; D002352:Carrier Proteins; D004247:DNA; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins; D008099:Liver / Q000378:metabolism*; D008854:Microscopy, Electron; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D013539:Simian virus 40; D012965:Sodium Chloride / Q000494:pharmacology
D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012965:Sodium Chloride; D004247:DNA