Identification of collagenase in cultured blood mononuclear cells.
Mononuclear cells isolated from peripheral whole blood were fractionated by erythrocyte-rosetting into T cells and non-T cell subpopulations which were tested for collagenase-producing capacity. Mononuclear cells and purified T lymphocytes, as well as non-T (B) lymphocytes, produced collagenase during serum-free culture. Removal of the macrophages from the non-T cells did not affect collagenase production by lymphocytes.
D001402:B-Lymphocytes / Q000201:enzymology; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology; D003012:Microbial Collagenase / Q000097:blood* / Q000378:metabolism; D009000:Monocytes / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology; D014783:Viscosity
D010447:Peptide Hydrolases; D003012:Microbial Collagenase