HeLa ribosomal protein S6. Insulin and dibutyryl cyclic AMP affect different phosphopeptides.
Tryptic phosphopeptides from HeLa ribosomal protein S6 have been separated by chromatography, followed by electrophoresis. Three phosphopeptides were found in S6 from cells treated in vivo with dibutyryl cAMP and at least four different phosphopeptides were found from cells treated with insulin plus the essential amino acids. Only phosphoserine was found in S6 isolated from insulin-treated cells.
D000596:Amino Acids / Q000032:analysis; D003994:Bucladesine / Q000494:pharmacology*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D010446:Peptide Fragments / Q000032:analysis; D010748:Phosphopeptides / Q000032:analysis; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014357:Trypsin
D000596:Amino Acids; D007328:Insulin; D010446:Peptide Fragments; D010748:Phosphopeptides; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D003994:Bucladesine; D014357:Trypsin