title: Correlation between growth rate, cell density, and intracellular concentrations of cyclic nucleotides in chemostat cultures of mouse L1210 cells.
abstract: Constant intracellular concentrations of both adenosine 3',5' cyclic monophosphate (cyclic AMP) and guanosine 3',5' cyclic monophosphate (cyclic GMP) were obtained when mouse leukemia L1210 cells were cultivated under steady-state conditions in the chemostat. When L1210 cells were maintained at a constant growth rate in the chemostat, the mean steady-state intracellular concentration of cyclic AMP decreased with increasing cell density, while the concentration of cyclic GMP remained unchanged. When cell growth rate was increased by three-fold, independently of cell density, in the chemostat, then the mean steady-state intracellular concentration of cyclic GMP increased by 3.6-fold, whereas that of cyclic AMP increased by only 22%. The ratio of cyclic AMP to cyclic GMP was found to decrease with increasing cell growth rate in the chemostat. Our results show that a close correlation exists between the steady-state intracellular concentrations of cyclic nucleotides and changes in both cell growth rate and cell density in chemostat cultures of L1210 tumour cells.
mesh_terms: D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D007700:Kinetics; D007939:Leukemia L1210; D051379:Mice
chemical_list: D000242:Cyclic AMP; D006152:Cyclic GMP