[DNA from cells infected with herpes simplex virus and from human tumors as an inducer of synthesis of a new nonvirion antigen].
DNA from herpes simplex virus type II (HSV-II)-infected cells isolated by using SDS, phenol, and pronase as well as by a new method of Pignatti et al. from triton X-100-NaCl-supernatant was shown to be capable to induce production of an early nonvirion (new) antigen in sensitive systems of Vero, RS-537 cells and others. It was also established that as a result of transfection of DNA recovered from 13 speciment of cancer tumors of the cervix and ovaries (9 tumors contained the new antigen and 4 were free from it), the new antigen associated with HSV-II was synthesized in 8 cases in the sensitive cells as early as 6 hours postinoculation whereas no viral antigens could be detected by this time. No viral antigens could be detected within 24 hours either, with the exception of 3 cases where CPE also developed by 24 hours indicating the appearance of the virus. DNA recovered from normal tissues of the cervix was incapable of inducing the new antigen.
D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000276:immunology*; D004279:DNA, Viral / Q000276:immunology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010051:Ovarian Neoplasms / Q000276:immunology*; D018139:Simplexvirus / Q000254:growth & development / Q000276:immunology*; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000276:immunology*; D014779:Virus Replication
D000941:Antigens; D004273:DNA, Neoplasm; D004279:DNA, Viral
DNK iz infitsirovannykh virusom prostogo gerpesa kletok i opukholeĭ cheloveka kak induktor sinteza novogo nervirionnogo antigena.