The effect of aging on beta-adrenoceptor-stimulated cyclic AMP formation in human lymphocytes.
1. Responsiveness of the beta-adrenoceptor adenylate cyclase system was measured in lymphocytes from healthy young and old subjects by incubating the cells with isoprenaline in the presence of a phosphodiesterase inhibitor and by measuring production of adenosine 3':5'-cyclic monophosphate (cyclic AMP) with a competitive binding assay. 2. The two groups did not differ significantly in the levels of cyclic AMP produced or in the concentration of isoprenaline required to give half-maximal stimulation of the cells (ED50).
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D013268:Stimulation, Chemical
D000242:Cyclic AMP; D007545:Isoproterenol