The use of markers in immune electron microscopy.
Immune electron microscopy (IEM) cannot be used successfully for structures that do not have recognisable morphology. However, at least some of these structures or components are related antigenically to recognisable antigens or viruses. We have therefore mixed unknown antigens with known markers and looked for the presence of mixed aggregates. The present study examined a low molecular weight subunit of rotavirus and a micellar form of hepatitis B surface antigen. In both cases mixed immune aggregates were found showing that the unknown components had antigens in common with the established virus or antigen.
D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis*; D002213:Capsid / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D008854:Microscopy, Electron; D012087:Reoviridae / Q000276:immunology*; D012401:Rotavirus / Q000032:analysis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D006514:Hepatitis B Surface Antigens