title: Effect of Cibacron Blue F3GA on phosphoglycerate kinase of Lactobacillus plantarum and phosphoglycerate mutase of Leuconostoc dextranicum.
abstract: Blue dextran or Cibacron Blue F3GA has been shown to inhibit yeast phosphoglycerate kinase [EC 2.7.2.3] competitively with respect to ATP (Thompson et al. (1975) Proc. Natl. Acad. Sci. U.S. 72, 663--667; Beissner and Rudolph (1979) J. Biol. Chem. 254, 6273--6277). However, we have found that phosphoglycerate kinase of Lactobacillus plantarum was inhibited by Cibacron Blue F3GA, the blue chromophore of blue dextran, noncompetitively with respect to ATP, but competitively with respect to 3-phosphoglycerate. Further inhibition studies with Cibacron Blue F3GA suggest that one molecule of the dye was bound per molecule of phosphoglycerate kinase at a saturated level of either substrate, but two molecules of the dye were bound per molecule of the kinase with an unsaturated level of either substrate used as a fixed substrate. Furthermore, phosphoglycerate mutase [EC 2.7.5.3] of Leuconostoc dextranicum was also inhibited by Cibacron Blue F3GA competitively with respect to 3-phosphoglycerate and noncompetitively with respect to 2,3-bisphosphoglycerate. These results suggest that the 3-phosphoglycerate-binding site on both phosphoglycerate kinase and phosphoglycerate mutase can interact with Cibacron Blue F3GA.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000873:Anthracenes / Q000494:pharmacology*; D001665:Binding Sites; D004396:Coloring Agents / Q000494:pharmacology*; D005988:Glyceric Acids / Q000378:metabolism; D007778:Lactobacillus / Q000201:enzymology*; D007934:Leuconostoc / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000378:metabolism*; D010736:Phosphoglycerate Mutase / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011485:Protein Binding; D014227:Triazines
chemical_list: D000873:Anthracenes; D004396:Coloring Agents; D005988:Glyceric Acids; D009943:Organophosphorus Compounds; D014227:Triazines; C012842:Cibacron Blue F 3GA; C005156:3-phosphoglycerate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D010735:Phosphoglycerate Kinase; D010736:Phosphoglycerate Mutase