Mitogenic response of C3H/HeJ mouse lymphocytes to polyanionic polysaccharides obtained from Bordetella pertussis endotoxin and from other bacterial species.
Lipopolysaccharide extracted from Bordetella pertussis was mitogenic for spleen cells of endotoxin-resistant C3H/HeJ mice. Although endotoxic lipid A was inactive, mitogenic activity of lipopolysaccharide was exhibited by purified preparations of polysaccharides I and II, which constitute the carbohydrate moiety of the macromolecule. These low-molecular-weight (2,800 and 3,600) polysaccharides, containing carboxyl groups, were not mitogenic for thymocytes and splenic T-cells of C3H/HeJ mice, but did show mitogenic activity for splenic B-cells of C3H/HeJ mice and for spleen cells of C57BL/6 athymic nude mice. The mitogenic activities of polysaccharides I and II were also compared with those of other polyanionic polysaccharides, and the results indicate that high molecular weight is not necessary, and negative charges are not sufficient, for mitogenicity.
D000818:Animals; D000838:Anions; D001402:B-Lymphocytes / Q000166:cytology; D001886:Bordetella pertussis; D004731:Endotoxins / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008934:Mitogens; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology
D000838:Anions; D004731:Endotoxins; D008934:Mitogens; D011135:Polysaccharides, Bacterial