Persistent infection of a cell line of mouse origin after cell fusion by u.v.-inactivated Sendai virus.
A cell line derived from Sendai virus-induced fusion of human adenocarcinoma and CBA mouse embryo cells had Sendai virus antigen (detected by immunofluorescence), together with bi-armed marker chromosomes, in 100% of the cells. After repeated passage, antigen-free cells carrying the same marker chromosomes appeared in the culture. Acrylamide gel analysis showed that all the Sendai virus antigens of antigen-positive cells were normal with the exception of the M protein. Antigen-negative cells contained no virus proteins and could be superinfected with wild-type virus, when all virus proteins appeared.
D000230:Adenocarcinoma; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D006801:Humans; D007621:Karyotyping; D051379:Mice; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development* / Q000276:immunology; D012004:Rectal Neoplasms; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
D000956:Antigens, Viral; D014764:Viral Proteins