Electron microscopic identification and subsequent isolation of a rotavirus from a dog with fatal neonatal diarrhea.
Negative contrast electron microscopic examination of intestinal contents from a 3-day-old pup with fatal diarrhea exhibited numerous rotavirus-like particles. Rotavirus was subsequently recovered, using MDCK cells, and was carried through 11 sub-passages; rotavirus-like particles were identified in each passage by negative contrast electron microscopic examination. Experimental inoculation of two 6-month-old Beagle dogs with purified intestinal contents did not result in clinical signs or virus shedding.
D000818:Animals; D000831:Animals, Newborn; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D008854:Microscopy, Electron; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary*