Effect of 3-O-methyl-D-glucose on the production of glycosidases by Cryptococcus laurentii and Saccharomyces cerevisiae.
3-O-Methyl-D-glucose (3-O-MeGlc) or a mixture of 3-O-MeGlc and glucose stimulate invertase, beta-glucosidase, alpha-glucosidase, and alpha-galactosidase production by Cryptococcus laurentii. They also increase invertase and alpha-glucosidase production by Saccharomyces cerevisiae. The stimulatory effect of 3-O-MeGlc is not caused by competition with glucose for transport nor by a direct action on glycosidases. It is proposed that 3-O-MeGlc acts as a structural rather than as a functional analogue of glucose displacing it from regulatory sites to relieve catabolite repression. Evidence is presented suggesting that intracellular cAMP levels may be related to the effect of 3-O-MeGlc.
D019325:3-O-Methylglucose; D003454:Cryptococcus / Q000201:enzymology*; D000242:Cyclic AMP / Q000502:physiology; D004790:Enzyme Induction / Q000187:drug effects; D005959:Glucosidases / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D008757:Methylglucosides / Q000494:pharmacology*; D008759:Methylglycosides / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013393:Sucrase / Q000096:biosynthesis; D000519:alpha-Galactosidase / Q000096:biosynthesis
D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose; D000242:Cyclic AMP; D005959:Glucosidases; D006026:Glycoside Hydrolases; D000519:alpha-Galactosidase; D013393:Sucrase