title: Identification of the avian myeloblastosis virus genome. I. Identification of restriction endonuclease fragments associated with acute myeloblastic leukemia.
abstract: The proviral DNA of chicken peripheral blood leukemic myeloblasts was analyzed by restriction endonuclease digestion and Southern blotting. Two restriction endonuclease-generated fragments, an EcoRI 2.2-megadalton (Md) and a HindIII 2.6-Md fragment, were present upon enzyme cleavage of all leukemic myeloblast DNA preparations in addition to endogenous or helper-specific fragments. Neither of these fragments was derived from viral DNA of the two known myeloblastosis-associated viruses (MAV-1 and MAV-2). In contrast, DNA similarly treated from the erythrocytes of leukemic chickens showed only small amounts of the two avian myeloblastosis virus-specific fragments, whereas the helper virus-specific fragments were present in the amount seen in MAV-producing chicken embryo fibroblasts. The appearance of the EcoRI 2.2-Md and HindIII 2.6-Md specific fragments in all leukemic myeloblast DNA preparations indicates they are presumably part of the leukemogenic genome that must be present to induce acute myeloblastic leukemia.
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000097:blood / Q000382:microbiology*; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D012526:Satellite Viruses / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes