An interchangeable ELISA for cytomegalovirus antigen and antibody.
An enzyme-linked immunosorbent assay (ELISA) was developed to demonstrate cytomegalovirus (CMV) antibodies and antigen. A nuclear antigen from CMV-infected cells was used for detection of IgG and IgM antibodies. Significant rises of CMV-IgG and significant levels of CMV-IgM were found in sera from patients with acute CMV only, and not with other herpesvirus infections. CMV antigens were demonstrated in cells and in culture medium by a sandwich or by an inhibition ELISA technique. In the sandwich technique, the plates were coated with monkey anti-CMV IgG and rabbit anti-CMV IgG was used as the second antibody. Both early and late CMV antigens were identified with this method. Treatment of CMV containing samples using freeze--thawing or detergent reduced the infectivity, but the antigenicity was not affected. There was no cross-reactivity with herpes simplex or varicella-zoster virus.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M