Transformations of 5,8,11,14,17-eicosapentaenoic acid in human platelets.
5,8,11,14,17-[1-14C]Eicosapentaenoic acid was prepared biosynthetically from [1-14C]arachidonic acid using the fungus, Saprolegnia parasitica. Incubation of 5,8,11,14,17-[1-14C]eicosapentaenoic acid with suspensions of human platelets led to the formation of three labeled compounds which were identified as thromboxane B3 (2-5% yield), 12-hydroxy-5,8,10,14-heptadecatetraenoic acid (2-5% yield), and 12-hydroxy-5,8,10,14,17-eicosapentaenoic acid (7-59% yield).
D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D000242:Cyclic AMP / Q000378:metabolism*; D004537:Eicosanoic Acids / Q000096:biosynthesis / Q000097:blood* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000097:blood* / Q000378:metabolism; D006801:Humans; D009868:Oomycetes / Q000378:metabolism; D011463:Prostaglandins H / Q000494:pharmacology
D001095:Arachidonic Acids; D004537:Eicosanoic Acids; D005231:Fatty Acids, Unsaturated; D011463:Prostaglandins H; C026222:12-hydroxy-5,8,10,14-heptadectetraenoic acid; C026221:12-hydroxy-5,8,10,14,17-eicospentaenoic acid; D015118:Eicosapentaenoic Acid; D000242:Cyclic AMP