The bovine genes for phosphoglycerate kinase, glucose-6-phosphate dehydrogenase, alpha-galactosidase, and hypoxanthine phosphoribosyltransferase are linked to the X chromosome in cattle-mouse cell hybrids.
Bovine embryonic trachea cells were hybridized with mouse A9 cells deficient in hypoxanthine phosphoribosyltransferase, and cattle-mouse hybrid cells clones were isolated after HAT/ouabain selection. In these interspecific cell hybrids, bovine glucose-6-phosphate dehydrogenase, alpha-galactosidase, and phosphoglycerate kinase were expressed concordantly with bovine HPRT. Their expression depended on the presence of bovine X chromosome. These data indicated that the genes for G6PD, PGK, and HPRT are linked and can be assigned to the bovine X chromosome.
D000818:Animals; D002417:Cattle / Q000235:genetics*; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D008040:Genetic Linkage; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006822:Hybrid Cells / Q000648:ultrastructure; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012730:Sex Chromosomes / Q000648:ultrastructure; D014960:X Chromosome / Q000648:ultrastructure; D000519:alpha-Galactosidase / Q000235:genetics*
D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase; D005696:Galactosidases; D000519:alpha-Galactosidase