Analysis of lymphokine producing T helper cells at the clonal level.
The quantitative representation of alloreactive helper T lymphocytes (HTL) within murine Lyt 1+2- cells has been analysed by the limiting dilution approach. Using three different experimental protocols evidence is presented that the HTL limiting the overall generation of both T help and IL-2 production may be distinct from the actual HTL providing T help or producing IL-2. While the former most likely represents the T inducer cell occurring at a frequency of about 1/2000 within Lyt 1/2- cells, the frequency of the latter is estimated to be about 1/200-1/500.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000950:Antigens, Ly; D007376:Interleukin-2; D007519:Isoantigens; D008222:Lymphokines; D003208:Concanavalin A