Synchronization of cell division in microorganisms by percoll gradients.
We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.
D001412:Bacillus subtilis / Q000166:cytology; D002455:Cell Division; D002499:Centrifugation, Density Gradient; D003102:Colloids; D003584:Cytological Techniques; D004926:Escherichia coli / Q000166:cytology; D008828:Microbiological Techniques; D011205:Povidone; D012568:Schizosaccharomyces / Q000166:cytology; D012822:Silicon Dioxide
D003102:Colloids; D012822:Silicon Dioxide; D011205:Povidone