[3H]-2-deoxyglucose autoradiography in a molluscan nervous system.
We have used [3H]2-deoxyglucose autoradiography to correlate the labeling of individual neurons with electrical activity within the central nervous system of a terrestrial mollusc, Limax maximus. In an electrically quiescent control preparation where a single neuron is impaled with a glass microelectrode but not stimulated, several somata are uniformly labeled at 3-5 times background. In preparations where a single cell is impaled and stimulated, one or more somata are heavily labeled with [3H]2-deoxyglucose at 10-50 times tissue background. This technique may be useful for surveying metabolically active neurons during spontaneous and driven electrical activity.
D000818:Animals; D001345:Autoradiography; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004558:Electric Stimulation; D005724:Ganglia / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D008974:Mollusca; D009474:Neurons / Q000502:physiology; D010525:Peripheral Nerves / Q000502:physiology; D009435:Synaptic Transmission; D014316:Tritium
D003837:Deoxy Sugars; D014316:Tritium; D003847:Deoxyglucose; D005947:Glucose