Neoplastic growths in chickens treated with cell and cell-free material from transplantable hepatoma induced by virus strain MC-29.
Series of experiments were carried out with chickens (line 15-I and Canadian white leghorn) for establishing the oncogenic properties of Mc-29 virus strain after its multiple replication in cells transformed by it (hepatoma cells). It was established that: 1. After s. c. and i. m. inoculation of intact hepatoma cells local hepatomas of varying size developed after 8--15 days accompanied with general viremia; after i. p. or i. v. injection multiple hepatoma growths occurred in the visceral organs and bone marrow. 2. The i. v. or i. p. inoculation of cell-free material of hepatoma or blood plasma of hepatoma bearing chicken lead after 48--80 days to the formation of hemocytoblastic and myelocytoma growths in the visceral organs and in the bone marrow and exclusively rare--of primary liver and kidney carcinomas. After s. c. injection of cell-free material the result is negative. A conclusion could be drawn that strain Mc-29 virus though replicated in the transformed hepatoma cells preserved its basic leukemogenic potentialities to induce myelocytomatosis and hemocytoblastosis and very rarely--liver and kidney carcinomas.
D000367:Age Factors; D000818:Animals; D001353:Avian Leukosis / Q000473:pathology; D001354:Avian Leukosis Virus; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002645:Chickens; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D013997:Time Factors; D014184:Transplantation, Homologous