[A thermosensitive inhibitor of the replication of polyomavirus, an extract from BHK2I cells transformed by the Tsa mutant of this virus].
BHK21 cells transformed by wild type or Ts3 mutant polyoma virus contain an inhibitor of polyoma virus replication when grown at permissive (36 degrees C) as well as non-permissive temperature (39 +/- 0.5 degrees C). Cells transformed by the Tsa mutant contain the inhibitor at the permissive but not at the non-permissive temperature. The inhibitor reappears in the latter cells however, upon shift from the non-permissive to the permissive temperature. If a reversible protein inhibitor (methionyl-adenylate, reversible inhibitor of the aminoacyl-t-RNA synthetase) is applied during the temperature shift experiments, the inhibitor does not reappear indicating that new protein synthesis is required for the recovery of its activity.
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology*; D002472:Cell Transformation, Viral; D006224:Cricetinae; D008647:Mesocricetus; D051379:Mice; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics*; D013696:Temperature; D014020:Tissue Extracts / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D002457:Cell Extracts; D014020:Tissue Extracts
Un inhibiteur thermosensible de la réplication du virus polyome, extrait de cellules BHK21 transformées par le mutant Tsa de ce virus.