[Activation of Na, K-ATPase from nerve cell microsomes by acetylcholine in a model system].
Microsomal Na, K-ATPase is activated by acetylcholine (5 x 10(-6)--10(-5) M) in a cell-free system including neuronal nuclei and the microsomal--cytoplasmic fraction. No enzyme activation by acetylcholine occurs in the presence of puromycin, actinomycin D and ribonuclease or upon removal of the nuclear or microsomal--cytoplasmic fraction from the system. After preincubation with acetylcholine the membranes reveal a better capacity for phosphorylation by [gamma-32P]ATP and dephosphorylation in the presence of ADP and Na+. The ATP binding by the membranes preincubated in a system with acetylcholine is also increased thereby. It was assumed that acetylcholine induces the synthesis of Na, K-ATPase or its protein activator.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation; D005260:Female; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology* / Q000648:ultrastructure; D008954:Models, Biological; D009474:Neurons / Q000201:enzymology*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D003609:Dactinomycin; D011691:Puromycin; D012260:Ribonucleases; D000254:Sodium-Potassium-Exchanging ATPase; D000109:Acetylcholine
Aktivatsiia Na,K-ATPazy mikrosom nervnykh kletok atsetilkholinom v model'noĭ sisteme.