Kinetics of nuclease digestion of Physarum polycephalum nuclei at different stages of the cell cycle.
The kinetics of nuclease digestion of Physarum polycephalum nuclei by staphylococcal nuclease and DNase I has been studied at different stages of the cell cycle. Significant differences in the digestion behaviour of nuclei from metaphase and interphase have been detected with DNase I but not with staphylococcal nuclease. Furthermore the structure of newly replicated DNA in S phase differs from the bulk in that it is more easily degraded to acid-soluble products by either staphylococcal nuclease or by DNAase I. At least four types of chromatin structure can be distinguished by our digestion kinetics experiments.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism / Q000502:physiology*; D002843:Chromatin / Q000648:ultrastructure; D004261:DNA Replication; D004271:DNA, Fungal / Q000032:analysis*; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D007700:Kinetics; D008836:Micrococcal Nuclease / Q000378:metabolism*; D008854:Microscopy, Electron; D009707:Nucleosomes / Q000648:ultrastructure; D010804:Physarum / Q000254:growth & development* / Q000378:metabolism
D002843:Chromatin; D004271:DNA, Fungal; D009707:Nucleosomes; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease