title: Recognition sequence of bacteriophage phi X174 gene A protein--an initiator of DNA replication.
abstract: Gene A protein, the initiator protein of bacteriophage phi X174 DNA replication, cleaves synthetic single-stranded oligodeoxyribonucleotides at the same site as the corresponding sequence at the phi X origin. The results identify the recognition sequence within the decamer CAACTTGATA which is cleaved next to the G residue. Further requirements for cleavage of double-stranded DNA by the gene A protein are supercoiling and an A + T-rich domain adjacent to the recognition sequence.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism; D005814:Genes, Viral; D013379:Substrate Specificity; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004720:Endonucleases