BK virus DNA sequence coding for the t and T antigens and evaluation of methods for determining sequence homology.
The DNA sequence of the early region of the human papovavirus BK (MM strain) was determined. A potential initiation signal for translation is located at nucleotides 3,047 to 3,045 or map position 0.614. Extending counterclockwise from this AUG signal there is only one open reading frame, which can code for a putative t antigen of 100 amino acids in length. If the early mRNA of BKV is spliced, then the regions between nucleotides 3,047 to 2,808 and 2,725 to 884 can code for a T antigen 694 amino acids in length. The sequences of the deduced T antigens in BK virus share 71% amino acid homology with those in simian virus 40, whereas the coding sequences of the two viruses share 70% DNA homology. Comparison of DNA sequences and evaluation of homology measurements between these two viruses are discussed.
D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics*; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics*; D001739:BK Virus / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003062:Codon; D004279:DNA, Viral / Q000235:genetics*; D005815:Genetic Code; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000235:genetics*; D013539:Simian virus 40 / Q000032:analysis
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D003062:Codon; D004279:DNA, Viral