Biochemical characterization of simian foamy virus type i.
Simian syncitium-forming ("foamy") virus type I (SFV1) was characterized biochemically. RNA was extracted from purified virus either with 0.1 per cent SDS or by the standard phenol-chloroform method. By both techniques a main component of 65-70S was found. Denaturation of the 65-70S RNA by heat resulted in a shift of the sedimentation coefficient mainly to a 30-35S component. Electrophoresis on a composite polyacrylamide gel demonstrated the existence of three minor RNA's: 8S, 5S and 4S respectively. PAGE-SDS analysis of disrupted purified virions enabled the separate migration of five viral proteins and the identification of two main proteins: a 30 kd polypeptide and a 70 kd polypeptide.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002520:Cercopithecidae / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D012367:RNA, Viral / Q000378:metabolism*; D012190:Retroviridae / Q000378:metabolism*; D016092:Spumavirus / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
D012367:RNA, Viral; D014764:Viral Proteins