Properties of mouse embryo fibroblasts transformed in vitro by infectious bovine rhinotracheitis virus.
Infection of CD-1 mouse embryo fibroblast(s) (MEF) with infectious bovine rhinotracheitis virus (IBRV) strain HMC resulted in persistent infection and subsequent transformation of these cells. IBRV-transformed MEF cultures consisted of short fibroblastoid cells, and IBRV-specific membrane and intracellular antigens were detectable in early in vitro passages by indirect imunofluorescence (IF) techniques. The presence of IBRV genetic information was confirmed in IF-positive and IF-negative cells by in situ hybridization. IBRV-transformed MEF induced fibrosarcomas in athymic nude mice given sc transplants. Infectious virus could not be rescued from the transformed cells or from tumor cells by cocultivation with rabbit kidney cells, by treatment with 5-iodo-2'-deoxyuridine, or by UV irradiation. Nontransformed control cells did not survive more than 10 in vitro passages and did not induce tumors when transplanted to athymic nude mice. These observations represent new data concerning the mouse cell-transforming potential of IBRV and confirm the presence of at least part of the virus genome in the transformants.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000473:pathology* / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005354:Fibrosarcoma / Q000473:pathology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000382:microbiology / Q000473:pathology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral