[(Na+, K+)-dependent adenosine triphosphatase in the rat brain, heart and kidney. Changes in the enzymatic metabolic quotient in purification with sodium deoxycholate].
The usage of detergents is included in most of the proposed methods for purification of a membrane bound enzyme (Na+, K+)-dependent adenosine triphosphatase (EK 3.6.1.3). The detergents influence the lipid envelope of an enzyme, which is considered as an integral part of the enzymic molecule, and extract nonactive proteins from the membrane. The aim of the work is measurement of the metabolic number of the enzyme after two-fold treatment with ionic detergent-sodium desoxycholate in brain, renal and heart tissue of a rat. He finds that the desoxycholate alters the metabolic number in the preparations from renal and brain tissue and does not change it in the heart tissue. These differences according to the author are due to differences in the membrane structures of the tissues. The usage of two-fold treatment of enzyme preparations with sodium desoxycholate is unsuitable for purification of (Na+, K+)-dependent adenosine triphosphatase in kidneys and brain.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification / Q000378:metabolism*
D008565:Membrane Proteins; D003840:Deoxycholic Acid; D000254:Sodium-Potassium-Exchanging ATPase
(Na+, K+)-zavisima adenozin trifosfataza ot mozŭk, sŭrtse i bbrek na plŭkh. Promeni v ŏbmennoto chislo na enzima pri prechistva ne s natriev dezoksikholat.