Evidence for FSH-like material in ACTH granules of certain corticotropic cells in the pituitary of the pig.
The ultrastructural immunocytochemical peroxidase-antiperoxidase technique used on serial sections reveals that certain corticotropic cells contain both ACTH and FSH beta, but not LH beta (ACTH/FSH cells). To determine the specificity of the anti-FSH beta staining in these cells, immunocytochemical absorption experiments were performed. The results indicate that (1) anti-FSH beta and -ACTH antisera are bound to different antigens in the corticotropic cell, and (2) anti-FSH beta staining is specific for a FSH-like antigen. In the ACTH/FSH cells both hormones are stored in the same secretory granules, distributed among other granules that contain only ACTH.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D003594:Cytoplasmic Granules / Q000032:analysis*; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000648:ultrastructure; D013552:Swine / Q000378:metabolism*
D000324:Adrenocorticotropic Hormone; D005640:Follicle Stimulating Hormone