Molecular cloning of EcoRII endonuclease and methylase genes.
The genes for restriction-modification system EcoRII have been cloned from plasmid N3 DNA using RSF2124 as a vector plasmid. The hybrid plasmids designated pFK321 and pFK322 contained a 5.8 megadaltons EcoRI--fragment derived from N3 DNA including the genes for restriction-modification system EcoRII and a gene for resistance to sulfanilamide.
D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008780:Methyltransferases / Q000235:genetics*; D010957:Plasmids; D013424:Sulfanilamides / Q000494:pharmacology
D013424:Sulfanilamides; D008780:Methyltransferases; D004262:DNA Restriction Enzymes