Interaction between fluorescent-labeled ACTH1-24, isolated fat cells, and serum albumin.
Part of all of the difference in the ability of ACTH1-24, and a fluorescent-labeled ACTH1-24 to activate lipolysis in fat cells can be accounted for by label-related binding to albumin present in the assay medium.
D000273:Adipose Tissue / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D003619:Dansyl Compounds / Q000378:metabolism*; D008053:Lipid Mobilization / Q000187:drug effects; D008297:Male; D010446:Peptide Fragments; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003619:Dansyl Compounds; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D000324:Adrenocorticotropic Hormone