title: Modification of avian sarcoma proviral DNA sequences in nonpermissive XC cells but not in permissive chicken cells.
abstract: For the first time, we present evidence with restriction enzymes HpaII and MspI which indicates that the proviral DNA sequence of avian sarcoma virus is modified by methylation in a nonpermissive rat cell line but not in permissive chicken cells. Some of the endogenous viral sequences in the permissive cells were also methylated. No 5-methylcytosine could be detected in the unintegrated viral DNA.
mesh_terms: D044503:5-Methylcytosine; D001355:Alpharetrovirus / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D008745:Methylation; D051381:Rats; D011995:Recombination, Genetic
chemical_list: D004279:DNA, Viral; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes