Separation of cytochromes c by reversed-phase high-performance liquid chromatography.
Six kinds of cytochrome c of different origin, i.e., bovine, chicken, dog, horse, rabbit and tuna, were subjected to separation by reversed-phase high-performance liquid chromatography on three commercial packing materials; octadecyl-, octyl- and cyanoalkyl-silicas. The effects of reversed-phase material, mobile phase and temperature on the separation of cytochromes c were examined. The parameters of the mobile phase were the organic modifier, the pH, the salt concentration and additives. Under optimal conditions, five of the six cytochromes c were resolved in 10 min. The relative retention values cannot be explained in terms of the relative lipophilicities of the side-chains of the amino acid residues.
D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003574:Cytochrome c Group / Q000302:isolation & purification*; D004285:Dogs; D006736:Horses; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011817:Rabbits; D013045:Species Specificity; D013696:Temperature; D014413:Tuna
D003574:Cytochrome c Group