Solubilization of active opiate receptors.
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with a new type of detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, which is a zwitterionic derivative of cholic acid. The solubilized receptor complexes behave as large molecules with a Stokes radius of 70 A and contain protein as an essential constituent.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D003902:Detergents; D005033:Ethylmaleimide / Q000494:pharmacology; D009474:Neurons / Q000032:analysis*; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects / Q000302:isolation & purification*; D012995:Solubility; D014357:Trypsin / Q000378:metabolism
D003902:Detergents; D011957:Receptors, Opioid; D014357:Trypsin; D005033:Ethylmaleimide