[Isolation and characteristics of highly purified S1-nuclease from amylorizin P10X].
S1-nuclease was purified from the Soviet commercial enzyme amylorizin P10x prepared from the surface culture of Aspergillus oryzae. The enzyme yield was 33% of total activity. The molecular weight of the enzyme measured by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate was equal to 30,000. The enzyme showed high specificity to single-stranded DNA.
D001230:Aspergillus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D002384:Catalysis; D002848:Chromatography, DEAE-Cellulose; D004277:DNA, Single-Stranded; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000302:isolation & purification*; D008970:Molecular Weight; D011489:Protein Denaturation; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases
Vydelenie i kharakteristika vysokoochishchennoĭ S1-nukleazy iz amilorizina P10X.