Significance of the delta 5 and delta 4 steroidogenic pathways in the hamster preovulatory follicle.
Isolated hamster granulosa cells and theca from preovulatory follicles were incubated in vitro for 2 and 6 h in the absence/or presence of LH and steroid substrates. The purpose of the experiments was to determine, in theca, the relative activities of the delta 5 and delta 4 pathways under controlled conditions, and to compare the ability of granulosa cells and theca to form progesterone from exogenous pregnenolone. The results of the experiments show that the delta 5 pathway in theca predominates before and up to 2 h after LH stimulation. The delayed effect of LH after 2 h is a switch from delta 5 to delta 4 as the major metabolic pathway. Progesterone formation from exogenous pregnenolone is 7 to 10 times greater in unstimulated granulosa cells than in theca. Acute effects of LH lead to increased conversion of exogenous pregnenolone to progesterone in granulosa cells but not theca. LH does, however, acutely stimulate the thecal conversion of DHEA to androstenedione. The longer term effect of LH in both cell types is to increase pregnenolone conversion to progesterone.
D000735:Androstenedione / Q000096:biosynthesis; D000818:Animals; D006224:Cricetinae; D003687:Dehydroepiandrosterone / Q000096:biosynthesis; D013196:Dihydrotestosterone / Q000096:biosynthesis; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology; D008647:Mesocricetus; D006080:Ovarian Follicle / Q000378:metabolism*; D010060:Ovulation; D011284:Pregnenolone / Q000096:biosynthesis / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D013739:Testosterone / Q000096:biosynthesis; D013799:Theca Cells / Q000378:metabolism; D013997:Time Factors
D013196:Dihydrotestosterone; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D011374:Progesterone; D011284:Pregnenolone; D007986:Luteinizing Hormone