Plasmid vectors containing the tryptophan operon promoter suitable for efficient regulated expression of foreign genes.
Derivatives of plasmid pBR322 that are suitable for high-level expression of foreign genes have been constructed. The vectors contain the Escherichia coli tryptophan promoter, the trpE gene, and about 15% of the trpD gene. To obtain expression, foreign genes are fused to the trpD gene fragment. After induction of the trp operon with 3 beta-indolylacrylic acid, trp gene products increase at least 50-fold, to account for 55% of the newly synthesised protein and 30% of total protein in the cell.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002642:Chick Embryo; D004269:DNA, Bacterial / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D009876:Operon; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic; D013752:Tetracycline / Q000494:pharmacology; D014169:Transformation, Bacterial; D014364:Tryptophan / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014364:Tryptophan; D013752:Tetracycline