An improved viscosimetric assay for vertebrate collagenase activity.
An improved viscosimetric assay for vertebrate collagenase acitivity is described. The assay is carried out at 35 degrees C in the presence of 1 M glucose to prevent fibril formation. The decrease in viscosity is linear with ime and proportional to enzyme concentration.
D000818:Animals; D003094:Collagen / Q000032:analysis; D006801:Humans; D003012:Microbial Collagenase / Q000032:analysis*; D011817:Rabbits; D012216:Rheumatic Diseases / Q000201:enzymology*; D012867:Skin / Q000378:metabolism; D013582:Synovial Fluid / Q000201:enzymology*; D014783:Viscosity
D003094:Collagen; D003012:Microbial Collagenase