Method for recovering viruses from river water solids.
Small numbers of virions (poliovirus 1) that had been adsorbed to river water solids were eluted by mixing the solids for 30 min with a 10% solution of beef extract that contained sufficient Na2HPO4 to bring the molarity of the salt to 0.05 and sufficient citric acid to bring the pH to 7. The virions were recovered by inoculating the beef extract onto cell cultures. With this method, 39 to 63% of the poliovirions that had been adsorbed onto the river water solids were recovered.
D000327:Adsorption; D000818:Animals; D002021:Buffers; D002460:Cell Line; D003470:Culture Media; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques; D017955:Poliovirus / Q000254:growth & development / Q000302:isolation & purification*; D014771:Virion / Q000302:isolation & purification; D014871:Water Microbiology
D002021:Buffers; D003470:Culture Media