Early steroid metabolism by chick blastoderm in vitro.
Yolk free blastoderms of chick embryo were incubated 3 or 22 hours with labeled pregnenolone, progesterone, 17-hydroxyprogesterone, dehydroepiandrosterone, androstenedione, testosterone and estradiol-17 beta. Metabolites and unconverted substrates were found both in the incubation medium and in the cells. Enzymes responsible for identified conversions were: 17 alpha-hydroxylase, 17-20-desmolase, delta 5 3 beta- and 3 alpha-hydroxysteroid dehydrogenase, 17 beta-hydroxysteroid dehydrogenase and 5 alpha- and 5 beta-reductase. The results suggest that the steroid metabolizing enzyme activities found may reflect a more general ability of early embryonic cells.
D019326:17-alpha-Hydroxyprogesterone; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001756:Blastoderm / Q000378:metabolism*; D002642:Chick Embryo; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D006908:Hydroxyprogesterones / Q000378:metabolism; D007700:Kinetics; D011284:Pregnenolone / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism
D006908:Hydroxyprogesterones; D013256:Steroids; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol; D019326:17-alpha-Hydroxyprogesterone; D011284:Pregnenolone