title: Purification from rat sarcolemma of the saxitoxin-binding component of the excitable membrane sodium channel.
abstract: The saxitoxin-binding component (SBC) of the excitable membrane sodium channel has been solubilized and purified from rat skeletal muscle sarcolemma. Phospholipid was required in mixed micelles with detergent for stability of the mammalian SBC. Even at optimal detergent-to-phospholipid ratio, the solubilized SBC showed significant temperature-dependent loss of specific toxin binding with time, necessitating maintenance of low temperatures during purification. Characteristics of saxitoxin binding to the solubilized material closely resembled those seen in intact membranes. A weak anion-exchange column was synthesized; it provided rapid 10- to 20-fold purification of the solubilized SBC. Additional necessary purification was obtained by chromatography on immobilized wheat germ agglutinin. Specific saxitoxin-binding activity of the purified material averaged approximately 1500 pmol of saxitoxin bound per mg of protein. Three bands were present in this material on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The purified material sedimented on a sucrose gradient with an apparent s20,w of 9.9 S.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D003902:Detergents; D006023:Glycoproteins / Q000302:isolation & purification*; D007473:Ion Channels / Q000378:metabolism / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification; D051381:Rats; D012508:Sarcolemma / Q000032:analysis*; D012530:Saxitoxin / Q000378:metabolism*; D012995:Solubility
chemical_list: D003902:Detergents; D006023:Glycoproteins; D007473:Ion Channels; D008565:Membrane Proteins; D009124:Muscle Proteins; D012530:Saxitoxin