[Studies on the activity of the aryl hydrocarbon hydroxylase and metabolism of benzo(a)pyrene in cell strains and cell lines used for testing cytotoxic and transforming ability of foreign compounds (author's transl)].
The activity of the AHH and the formation of polar metabolites from BaP was examined in several cell strains and lines, which are used for testing cytotoxic and transforming activity of environmental agents, the highest activity of the AHH and the highest capacity to metabolize BaP was found in hamster kidney cells. Much lower values were measured in kidney cells from Cercopithecus aethiops, BHK21/C13 cells, and SV-40 transformed hamster kidney cells (14-1b). The activity of AHH was inducible by BA in all these cells. However no correlation could be established between the increase in AHH activity and the formation of polar metabolites. In cells derived from a human bronchial carcinoma (E-14), a very low BaP metabolism was detectable which increased 3-fold after exposure (o BA. No BaP metabolism could be detected in HeLa cells.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001580:Benzopyrenes / Q000378:metabolism*; D001984:Bronchial Neoplasms; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D006224:Cricetinae; D046508:Culture Techniques; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D013539:Simian virus 40
D001580:Benzopyrenes; D001189:Aryl Hydrocarbon Hydroxylases
Untersuchungen über die Aktivität der Aryl-hydrokarbon-Hydroxylase und den Benzo(a)pyrenabbau in Zellstämmen und Zellinien, die für Zytotoxizitäts- und Transformationstests verwendet werden.