Characterization of responding cells in oxidative mitogen stimulation. III. Presence of I-A- and I-J, E, C-subregion gene products on the surface of required cells.
Ia antigens coded by genes of the murine major histocompatibility complex are expressed on the surface of a population of cells critical to the proliferative response of murine spleen cells to the oxidative mitogen neuraminidase/galactose oxidase. By selective depletion with antiserum and complement, Ia antigens coded (or determined) by the I-A and I-J, E, C subregions of the Ir region can be detected on the surface of cells required for the response. In addition, I-A-subregion products have a functional significance in cellular activation which can be demonstrated by blocking experiments with anti-Ia serum in the absence of complement.
D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface; D001667:Binding, Competitive; D002874:Chromosome Mapping; D003165:Complement System Proteins; D005260:Female; D005692:Galactose Oxidase / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008934:Mitogens / Q000494:pharmacology*; D010504:Periodic Acid / Q000494:pharmacology; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D013154:Spleen / Q000378:metabolism*
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D008934:Mitogens; D010504:Periodic Acid; D003165:Complement System Proteins; D005692:Galactose Oxidase