Separation of angiotensin receptor from cell membrane angiotensinase in rabbit aorta.
Deoxycholic acid (0.01%) diminished the tissue response to angiotensin in rabbit aortic strips. A lower maximal response was obtained, but the affinity of angiotensin receptors did not change. Specific angiotensin receptor binding was measured in cell membrane fraction, solubilised fraction and pellet fraction after solubilization. The solubilised fraction showed threefold increase in receptor binding and was devoid of angiotensinase activity. The binding to pellet fraction after solubilization was increased or unchanged and contained the same angiotensinase activity as before solubilization. We conclude that it is possible to separate angiotensin receptor from cell membrane angiotensinase.
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000032:analysis*; D002462:Cell Membrane / Q000201:enzymology; D003840:Deoxycholic Acid / Q000494:pharmacology; D010450:Endopeptidases / Q000302:isolation & purification*; D005260:Female; D011817:Rabbits; D011945:Receptors, Angiotensin / Q000302:isolation & purification*; D011956:Receptors, Cell Surface / Q000302:isolation & purification*
D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D003840:Deoxycholic Acid; D000804:Angiotensin II; D010450:Endopeptidases