Use of the periodate oxidation coupling method for the detection of antibody and antibody-producing cells specific for staphylococcal lipoteichoic acid.
The periodate oxidation and chromium chloride coupling methods were compared for their ability to sensitize indicator erythrocytes with staphylococcal lipoteichoic acid (LTA) for the detection of specific antibody. Erythrocytes, sensitized with periodiate-activated lipoteichoic acid, were found to be superior for use in both passive immune hemagglutination and hemolysis tests as well as in the technique of localized hemolysis-in-gel for the detection of specific antibody-producing cells against LTA.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000921:Antibody-Producing Cells / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D008070:Lipopolysaccharides; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010712:Phosphatidic Acids / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology*
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D010712:Phosphatidic Acids; D013682:Teichoic Acids; D010504:Periodic Acid; C009900:lipoteichoic acid