Effects of D,L-alpha-aminoadipate on postsynaptic amino acid responses in cultured mouse spinal cord neurons.
The effects of the dicarboxylic amino acid, DL-alpha-aminoadipate (DLAA) on amino acid responses have been investigated using intracellular recordings from mouse spinal cord neurons grown in dissociated cell culture. DL-alpha-Aminoadipate markedly antagonized postsynaptic responses to iontophoretically appllied aspartate; antagonism of glutamate was much less prominent. DL-alpha-Aminoadipate altered the affinity of aspartate for its receptor while having no observed effects on aspartate-receptor cooperativity. No direct effects of DLAA on membrane potentials or passive membrane properties were seen at the currents used for antagonism. Responses to the inhibitory amino acids GABA and glycine were unaffected by DLAA.
D015074:2-Aminoadipic Acid / Q000494:pharmacology*; D000596:Amino Acids / Q000494:pharmacology*; D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology*; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D046508:Culture Techniques; D005071:Evoked Potentials / Q000187:drug effects; D005971:Glutamates / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D051379:Mice; D013116:Spinal Cord / Q000187:drug effects*; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D000596:Amino Acids; D000600:Amino Acids, Dicarboxylic; D005971:Glutamates; D015074:2-Aminoadipic Acid; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D005998:Glycine