title: Comparison of proliferation and cytopathogenicity of swine vesicular virus and coxsackievirus B5.
abstract: Sequential appearance of both swine vesicular disease virus and Coxsackievirus B5 antigens in a pig kidney cell line was studied by immunofluorescence and electron microscopy. The replication cycle of each virus was approximately 3-4 h. Viral antigens were demonstrable in the cytoplasm 2 h after inoculation. A compact mass of fluorescence was seen when cells showed cytopathogenic effect at 5.5 h. After 3 h, a few viral particles, seen by electron microscopy, were in the cytoplasm. Morphological changes of cells occurred at the same time. Cytoplasmic crystalline arrays of virus were first detected at 7 h.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000472:pathogenicity*; D029822:Enterovirus B, Human / Q000254:growth & development / Q000472:pathogenicity*; D004769:Enterovirus Infections / Q000662:veterinary*; D004771:Enteroviruses, Porcine / Q000254:growth & development / Q000472:pathogenicity*; D007668:Kidney; D013552:Swine; D013555:Swine Vesicular Disease / Q000382:microbiology*; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral