Immunization of inbred guinea pigs with varicella-zoster virus grown in a syngeneic transformed embryo cell line.
Varicella-zoster virus was serially propagated in a chemically transformed and cloned line of embryo cells (designated "104 C1") derived from the inbred strain 2 guinea pig. When strain 2 guinea pigs were immunized with varicella-zoster virus subcultivated in the syngeneic cell line, they produced high-titer virus-specific antiserum which lacked antibody against cellular determinants. This immunization procedure offers both practical and theoretical advantages over prior methods which involved inoculation of outbred laboratory animals with varicella-zoster virus grown in allogeneic or xenogeneic cell cultures.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D014645:Herpesvirus 3, Human / Q000254:growth & development / Q000276:immunology*; D007114:Immunization; D008297:Male; D009500:Neutralization Tests; D014776:Virus Cultivation / Q000379:methods*
D000914:Antibodies, Viral