Effects of pressure and temperature on the reactions of horseradish peroxidase with hydrogen cyanide and hydrogen peroxide.
Reactions of ferric horseradish peroxidase with hydrogen cyanide and hydrogen peroxide were studied as a function of pressure. Activation volumes are small and differ in sign (delta V = 1.7 +/- 0.5 ml/mol for peroxidase + HCN and -1.5 +/- 0.5 ml/mol for peroxidase + H2O2). The temperature dependence of cyanide binding to horseradish peroxidase was also determined. A comparison is made of relevant parameters for cyanide binding and compound I formation.
D004789:Enzyme Activation; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006856:Hydrogen Cyanide / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism*; D011312:Pressure; D013696:Temperature; D013816:Thermodynamics
D006856:Hydrogen Cyanide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases