Development of [3H]muscimol binding to subcellular particles of a culture of mouse brain.
"Specific" binding of [3H]muscimol (11.5 nM) related to GABA receptors, occurred in subcellular particles prepared from 4-, 8-, and 12-day cultures of embryonic (14- to 15-day-old) mouse cerebrum, but not to particles prepared from 20- to 45-day cultures. Phase-contrast microscopy revealed that the presence of neuronal elements (neuroblasts, neurites) paralleled the binding of [3H]muscimol. Further evidence is thus provided for using high-affinity [3H]muscimol binding as a "neuronal marker."
D000818:Animals; D001369:Axons / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009118:Muscimol / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010080:Oxazoles / Q000378:metabolism*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism; D011963:Receptors, GABA-A; D013347:Subcellular Fractions / Q000378:metabolism
D010080:Oxazoles; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D009118:Muscimol