The latex particle adherence assay: inhibitory effects of protein preparations.
The latex particle adherence to peripheral chicken lymphocytes in the presence of different concentrations of various protein preparations was examined. Non-specific inhibition of adherence was observed at and above concentrations of 500 microgram/ml, whereas protein concentrations at and below 250 microgram/ml gave no significant adherence inhibition in control chicken lymphocytes. The protein concentration of 250 microgram/ml of antigens prepared from MDV-infected chicken skin and HPRS-1 cells specifically inhibited the LPA to lymphocytes of MDV-infected chickens.
D000818:Animals; D000941:Antigens / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002448:Cell Adhesion; D002645:Chickens; D008381:Herpesvirus 2, Gallid / Q000276:immunology; D007840:Latex; D008214:Lymphocytes / Q000276:immunology; D008380:Marek Disease / Q000276:immunology*; D008863:Microspheres; D009363:Neoplasm Proteins / Q000276:immunology*; D011506:Proteins / Q000276:immunology*
D000941:Antigens; D000956:Antigens, Viral; D007840:Latex; D009363:Neoplasm Proteins; D011506:Proteins