5-Hydroxytryptamine releasing activity in culture supernatants of guinea pig lymphocytes.
5-Hydroxytryptamine (5-HT) is not only involved in anaphylactic reactions but also in delayed-type hypersensitivity (DTH) reactions. Supernatants of cultivated guinea pig spleen lymphocytes were investigated for possessing 5-HT releasing activity. Both fractionated supernatants of mixed lymphocytes unspecifically stimulated by Concanavalin A (Con A) and fractionated supernatants of non-stimulated mixed lymphocytes decrease the 5-HT content of rabbit thrombocytes. Fractions of a molecular weight smaller than 12,500 daltons are more effective than fractions of a higher molecular weight range. The 5-HT releasing activity of the supernatants is discussed in comparison to a cell migration stimulating activity.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle; D002464:Cell Migration Inhibition; D003208:Concanavalin A / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism*
D003208:Concanavalin A; D012701:Serotonin