Replicative potentials of various fusion products between WI-38 and SV40 transformed WI-38 cells and their components.
Hybrid cells derived from whole-cell fusions of replicating phase-II normal fibroblast cells (WI-38s) with SV40 transformed WI-38 fibroblast cells (CL-1s) demonstrated that the majority of the hybrid experimental cells still maintained a finite life-span. Approximately 2% demonstrated sustained and possibly indefinite replication. Experimental binucleate cells and subsequent hybrid synkaryons were also formed by fusing CL-1 karyoplasts into phase-II WI-38 replicating normal fibroblasts. In addition, viable cells were constructed from WI-38  fibroblast cytoplasts with CL-1 karyoplasts. Sustained replication was not observed in these crosses.
D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D008168:Lung; D013539:Simian virus 40; D013997:Time Factors