Properties of two temperature-sensitive Rous sarcoma virus transformed cerebellar cell lines.
Cells from the cerebellum of 3-day-old BD-IX rats were obtained as permanent lines by transforming them with temperature-sensitive Rous sarcoma virus. The presence or absence of veratridine-stimulated Na+-uptake (voltage-dependent channels) was used to operationally classify them as neuronal or glial. When incubated at 34 degrees C, the permissive temperature for transformation, the cerebellar cells exhibit a transformed phenotype determined by anchorage independence, rounded morphology, high growth rate and absence of density-dependent inhibition of growth. In contrast, when the transformed cerebellar cell lines are kept at a temperature (38 degrees C) non-permissive for transformation, they exhibit a normal cellular phenotype with respect to the above properties. Moreover, changes toward neuronal morphology, increase in veratridine-stimulated Na+-uptake, decreased growth rate and the expression of the astrocyte specific protein, glial fibrillary acidic protein, suggest that a degree of differentiation is expressed at the non-permissive temperature.
D000818:Animals; D001253:Astrocytes / Q000382:microbiology; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002531:Cerebellum / Q000382:microbiology*; D009474:Neurons / Q000382:microbiology; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013696:Temperature; D014701:Veratridine / Q000494:pharmacology
D014701:Veratridine; D012964:Sodium