Benzodiazepine receptors on primary cultures of mouse astrocytes.
Benzodiazepines bind to glial membranes on a single type of site, with a high affinity (KD = 5 x 10(-9) M) on about 100 fmol of sites per mg protein. The number of binding sites is increased when the membranes are treated with Triton X-100. Antiepileptic drugs such as clonazepam and phenobarbital and hypnotic drugs such as Ro-11-3128 and Ro-11-6896 are able in pharmacological concentrations to displace [3H]flunitrazepam from its glial binding sites.
D000818:Animals; D000927:Anticonvulsants; D001253:Astrocytes / Q000378:metabolism*; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003975:Diazepam; D005445:Flunitrazepam; D051379:Mice; D010634:Phenobarbital; D011955:Receptors, Drug / Q000378:metabolism*; D011963:Receptors, GABA-A
D000927:Anticonvulsants; D011955:Receptors, Drug; D011963:Receptors, GABA-A; D005445:Flunitrazepam; D003975:Diazepam; D010634:Phenobarbital