Release of lactate dehydrogenase during isolation of adult rat heart cells.
In order to determine the least injurious method of cell isolation, effluent perfusates from isolated rat hearts were examined for lactate dehydrogenase activity during cellular isolation procedures. Correlation of these results with the perfusates, with modifications in the perfusates, and with the yield of intact cells isolated, indicated that perfusion of isolated hearts with solutions containing bovine serum albumin and/or collagenase can result in severe cellular injury. These effects were significantly modified by the presence of calcium ion. These results indicated that the concentration of calcium ion during isolation is critical to successful isolation of calcium tolerant, functional adult heart cells.
D000818:Animals; D002122:Calcium Chloride; D002469:Cell Separation / Q000379:methods*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D003012:Microbial Collagenase; D009206:Myocardium / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase; D003012:Microbial Collagenase; D002122:Calcium Chloride