The effect of tris(hydroxymethyl) aminomethane on membrane bound 5'nucleotidase from swine aortic smooth muscle.
5'-Nucleotidase (5'-ribonucleotide phosphohydrolase, EC 3.1.3.5) activity was stimulated by increasing the concentration of Tris over the range of 1--100 mM. Significant differences were found when the kinetic parameters of the enzyme determined in Tris were compared to those determined in glycine. These data suggest that Tris interacts with 5'-nucleotidase and is therefore unsuitable for use in the assay of the enzyme.
D015720:5'-Nucleotidase; D000818:Animals; D001011:Aorta / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D009708:Nucleotidases / Q000378:metabolism*; D014325:Tromethamine / Q000494:pharmacology*
D014325:Tromethamine; D009708:Nucleotidases; D015720:5'-Nucleotidase