[Expression of the restriction fragment of phage T7 DNA in a cell-free transcription-translation system from E. coli].
The matrix activity of phage T7 DNA and the restriction fragment of phage T7 DNA containing gene 0,3 with a promoter was studied with respect to RNA and protein syntheses in a cell-free transcription-translation system from E. coli. A comparison of the protein products of cell-free synthesis was carried out, using polyacrylamide gel electrophoresis and a subsequent radiofluorography. Data from PAAG suggest that the restriction fragment codes the synthesis of a polypeptide, whose size is identical to that of a polypeptide synthesized on a corresponding gene in phage T7 DNA. A possible use of the cell-free system of coupled transcription-translation for the functional mapping of the genome DNA is discussed.
D002474:Cell-Free System; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D014176:Protein Biosynthesis; D013604:T-Phages / Q000378:metabolism*; D014158:Transcription, Genetic
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Ekspressiia restriktsionnogo fragmenta DNK faga T7 v beskletochnoĭ sisteme transkriptsii-transliatsii iz E. coli.