Epstein-Barr virus co-reconstituted with Sendai virus envelopes infects Epstein-Barr virus-receptor negative cells.
Epstein-Barr virus (EBV) was co-reconstituted with Sendai virus envelopes. The reconstituted "hybrid' virus could bind and penetrate into EBV-receptor negative cells. Using this approach, T-cell-derived human and mouse leukemia cells, human T-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of EBV-determined antigens and stimulation of DNA synthesis. The T-cell-derived human leukemia line Molt-4, that can absorb EBV but without virus penetration, could be also infected by the reconstituted EBV.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D006389:Hemagglutinins, Viral / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007938:Leukemia / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology; D051379:Mice; D008979:Moloney murine leukemia virus; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D011991:Receptors, Virus / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D011991:Receptors, Virus; D004247:DNA