[Biochemical evidence for integration of prokaryotic DNA into mammalian cells (author's transl)].
Experiments were undertaken to test for the stable integration of defined prokaryotic genes in mammalian cells. Thus lambda dvgal 88 plasmid DNA were transferred together with the herpes simplex virus type 1 thymidine kinase (TK) gene as a selectable marker to TK- mouse cells. DNA restriction and hybridization analysis of TK+ mouse cell clone DNA revealed (i) the presence of prokaryotic lambda dvgal 88 sequences in the mouse DNA; (ii) the stable integration of the prokaryotic DNA in mouse cell DNA under selective conditions; and (iii) the presence of lambda dvgal fragments in several copies per cell and their integration at different loci within the cell DNA.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005796:Genes; D051379:Mice; D009693:Nucleic Acid Hybridization; D011387:Prokaryotic Cells / Q000378:metabolism; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000378:metabolism
D004247:DNA; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes
Biochemischer Nachweis für die Integration prokaryoter DNA in Sügerzellen.