Calcium flux of erythrocytes in Duchenne muscular dystrophy.
The calcium flux of erythrocytes from patients with Duchenne muscular dystrophy (DMD) was measured. Passive influx was determined using erythrocytes incubated with lanthanum chloride as a specific inhibitor to calcium transport. There was no significant difference in calcium influx into cytosol and into membrane fractions between DMD and control age-matched boys. Influx of calcium was also determined using ATP-depleted erythrocytes incubated without the inhibitor. Again there was no significant difference between DMD and control values. Efflux of calcium was measured using erythrocytes loaded with calcium ionophore A23187. Calcium efflux speed was related to intracellular calcium concentration and the efflux reached a plateau at about 1 mM. There was no significant difference in calcium efflux rate as a function of intracellular calcium concentration between DMD and control.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D002118:Calcium / Q000097:blood*; D002648:Child; D003600:Cytosol / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D009136:Muscular Dystrophies / Q000378:metabolism*
D007473:Ion Channels; D000255:Adenosine Triphosphate; D002118:Calcium