Cyclic AMP surges during the dihydrotestosterone-induced growth-division cycle of rat seminal vesicle cells.
A single subcutaneous injection of the active androgen DHT (5 alpha-androstan-17 beta-ol-3-one or 5 alpha-dihydrotestosterone; 5 mg/Kg body weight) into rats 7 days after orchidectomy stimulated DNA synthesis in the seminal vesicle, which began between 30 and 35 hr after injection and peaked at 48 hr. Mitotic activity began between 35 and 45 hr after DHT injection and peaked around 55 hr. These proliferative responses appeared to be confined mainly to the epithelial cells of the seminal vesicles. The DHT injection did not rapidly (between 5 min and 2 hr) increase the cyclic AMP level, but it did result in two small later surges of the cyclic nucleotide; the first of which peaked between 18 and 28 hr, but well before the onset of DNA synthesis, and the second of which peaked between 48 and 60 hr, when both the DNA-synthetic and mitotic activities were near or at their peak values.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D005856:Germ-Free Life; D008297:Male; D051381:Rats; D012669:Seminal Vesicles / Q000166:cytology*; D013268:Stimulation, Chemical
D013196:Dihydrotestosterone; D000242:Cyclic AMP