title: Extracellular accumulation of L-glutamate in adenylyl cyclase deficient or cyclic AMP receptor protein deficient mutants of Escherichia coli.
abstract: An Escherichia coli cya mutant deficient in adenylyl cyclase and an E. coli crp mutant deficient in cyclic AMP receptor protein (CRP) accumulate substantial amounts of L-glutamate extracellularly when entering stationary phase of growth. The cya mutant grown in the presence of cyclic AMP accumulates little glutamate whereas the addition of cyclic AMP has no effect on glutamate accumulation in the crp mutant. It is proposed that an E. coli cell entering stationary phase requires a change in cell envelope structure which involes a cyclic AMP-CRP dependent process, and without this process the permeability of the cell membrane increases.
mesh_terms: D000262:Adenylyl Cyclases / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D009154:Mutation; D011953:Receptors, Cyclic AMP / Q000502:physiology*
chemical_list: D005971:Glutamates; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D000262:Adenylyl Cyclases