The isolation of Salmonella from jejunal and caecal lymph nodes of slaughtered animals.
One jejunal and one caecal lymph node were sampled from each of 50 cows, 40 yearling cattle, 25 sheep, 20 lambs and 45 pigs after slaughter. Salmonella, Clostridium perfringens and Staphylococcus aureus, all organisms which cause food poisoning in man, were sought by direct plating methods. The samples were also enriched and cultured for Salmonella. Organisms were cultured from 208 (58%) of the 360 lymph nodes; aerobic plate counts yielded up to 25,000 organisms per gram of tissue, although from most infected samples less than 1000 organisms per gram were cultured. Salmonella was isolated directly from 5% of samples, with counts up to 1,500 per gram. After enrichment Salmonella was isolated from nodes taken from 15 cows, 2 yearling cattle, one sheep and 8 pigs. Cl. perfringens was isolated from the caecal nodes of 2 yearling cattle and 2 pigs; S. aureus was not isolated from any sample. It was concluded that mesenteric lymph nodes may be a significant reservoir of Salmonella for transfer to meat and meat products.
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002432:Cecum; D003016:Clostridium perfringens / Q000302:isolation & purification; D007583:Jejunum; D008198:Lymph Nodes / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*; D012756:Sheep / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*