Further studies on the application of the micromethod of phagocytosis using cadmium microcrystals as phagocytosis particles.
The use of Cd microcrystals together with a monolayer technique using small quantities of whole blood offers several advantages for routine studies of phagocytosis. The original method by Miler was especially minimized with regard to the necessary quantity of serum. Homologous serum was replaced by heterologous guinea-pig serum and the method was simplified by the omission of opsonization. A comparison of the stimulatory effect of inactivated and native serum as well as the inhibition of phagocytosis by trypan blue allows us to characterize Cd-phagocytosis as complement-dependent.
D002104:Cadmium; D003460:Crystallization; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D008832:Microchemistry; D009895:Opsonin Proteins; D010587:Phagocytosis
D009895:Opsonin Proteins; D002104:Cadmium