Insulin permits parathyroid hormone stimulation of 1,25-dihydroxyvitamin D3 production in cultured kidney cells.
Primary cultures of chick kidney cells in serum free medium respond to PTH with increased production of 1,25(OH)2D3 only when exposed to insulin. The response of 1,25(OH)2D3 is maximal at 5 ng bPTH (1-34) per ml and decreases at higher hormone concentrations. Increased 1,25(OH)2D3 synthesis is not evident after 30 minutes exposure to bPTH and is maximal at 4-6 hours of treatment. Insulin does not increase the cyclic AMP response to PTH suggesting that whatever permissive role it is playing occurs beyond the generation of cyclic AMP.
D000818:Animals; D002117:Calcitriol; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000096:biosynthesis*; D004359:Drug Therapy, Combination; D006887:Hydroxycholecalciferols / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology*
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D007328:Insulin; D010281:Parathyroid Hormone; D000242:Cyclic AMP; D002117:Calcitriol