Characterization of polyoma viral DNA sequences in polyoma-induced hamster tumor cell lines.
The polyoma (PY) viral DNA sequences present in a series of hamster tumor cell lines were evaluated using the blotting technique of Southern ((1975) J. Mol. Biol. 98, 503-517). Remarkably, no cell line contained an intact distal portion of the early gene region which encodes a portion of the large T antigen. All cell lines examined contained the PY DNA Bum I fragment which contains most of the genetic information encoding PY small and middle T antigens, as well as the origin of viral DNA replication. These results provide an explanation for our previous observation that PY virus-induced hamster tumors do not contain the large species of T antigen.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D005814:Genes, Viral; D009374:Neoplasms, Experimental; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000032:analysis
D004279:DNA, Viral; D004262:DNA Restriction Enzymes