title: Effect of surface modifiers on an ectoenzyme: granulocyte 5'-nucleotidase.
abstract: Several agents that react with plasma membranes, namely the native lectins concanavalin A, Ricinus communis agglutinin, and wheat germ agglutinin, the modified lectin succinyl concanavalin A, and sodium meta-periodate, inhibited the ecto-5'-nucleotidase of intact guinea pig granulocytes. Stimulation of the enzyme was not observed at any lectin concentration. Inhibition by native lectins could be blocked or reversed by appropriate competing hapten sugars. In the case of concanavalin A, reversal could be achieved at 37 degrees C, but not at 5 degrees C. When lectins were used in combination with each other, the effects were found to be largely independent. However, when concanavalin A and R. communis agglutinin were applied together, complications arose because the former lectin binds to the latter as well as to the cell surface. To avoid some of the complexities inherent in studying intact cell 5'-nucleotidase and to gain additional information about the system, two broken cell enzyme preparations were also examined. The enzyme of plasma membrane-enriched fractions was inhibited by all five agents mentioned above. 5'-Nucleotidase solubilized in sodium deoxycholate was inhibited by the four lectins but stimulated by periodate. The effects of the surface modifiers on kinetic data for all three enzyme preparations are consistent with the hypothesis that direct interactions with the enzyme molecule give rise to changes in Vmax; interactions at membrane sites other than 5'-nucleotidase itself could cause increases in apparent Km values. Effects of interactions of ectoenzymes with plant lectins may serve as models for phenomena that result from cell-cell interactions or from interactions of animal cells with lectin-like components of the cellular environment.
mesh_terms: D015720:5'-Nucleotidase; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A / Q000031:analogs & derivatives / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects*; D006168:Guinea Pigs; D006241:Haptens / Q000494:pharmacology; D007700:Kinetics; D037102:Lectins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009708:Nucleotidases / Q000378:metabolism*; D010504:Periodic Acid / Q000494:pharmacology; D037121:Plant Lectins; D010940:Plant Proteins / Q000494:pharmacology; D014909:Wheat Germ Agglutinins
chemical_list: D006241:Haptens; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins; D010504:Periodic Acid; D003208:Concanavalin A; C019558:succinylconcanavalin A; C009288:metaperiodate; D009708:Nucleotidases; D015720:5'-Nucleotidase