Inhibitory effect on the formation of chlamydial inclusions in McCoy cells by seminal fluid and some of its components.
Our purpose was to determine the effect of semen and some of its components upon chlamydial inclusion (CI) count and cytopathogenic effect in cycloheximide-treated McCoy cells. Semen and spermine caused a dose-related decrease in the number of CI, using a standardized infectious dose of Chlamydia trachomatis, immunotype F. Lysozyme in certain concentrations stimulated CI formation, whereas the cations Cu++ and Zn++ produced a cytopathogenic effect and a dose-related reduction of the CI count, EDTA neutralized the effects of the cations and its addition to seminal fluid experimentally infected with C. trachomatis resulted in a greater number of CI than found in control cultures containing only semen.
D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000187:drug effects*; D003300:Copper / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D002479:Inclusion Bodies / Q000187:drug effects*; D008297:Male; D009113:Muramidase / Q000494:pharmacology; D010088:Oxidoreductases / Q000494:pharmacology; D012661:Semen / Q000032:analysis / Q000502:physiology*; D012756:Sheep; D015032:Zinc / Q000494:pharmacology
D003300:Copper; D004492:Edetic Acid; D010088:Oxidoreductases; D009113:Muramidase; D015032:Zinc