title: Insulin permits parathyroid hormone stimulation of 1,25-dihydroxyvitamin D3 production in cultured kidney cells.
abstract: Primary cultures of chick kidney cells in serum free medium respond to PTH with increased production of 1,25(OH)2D3 only when exposed to insulin. The response of 1,25(OH)2D3 is maximal at 5 ng bPTH (1-34) per ml and decreases at higher hormone concentrations. Increased 1,25(OH)2D3 synthesis is not evident after 30 minutes exposure to bPTH and is maximal at 4-6 hours of treatment. Insulin does not increase the cyclic AMP response to PTH suggesting that whatever permissive role it is playing occurs beyond the generation of cyclic AMP.
mesh_terms: D000818:Animals; D002117:Calcitriol; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000096:biosynthesis*; D004359:Drug Therapy, Combination; D006887:Hydroxycholecalciferols / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology*
chemical_list: D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D007328:Insulin; D010281:Parathyroid Hormone; D000242:Cyclic AMP; D002117:Calcitriol