L-Ethionine as an inducer of differentiation in human promyelocytic leukemia cells (HL-60).
The methionine analog, L-ethionine, induces morphological and biochemical changes in cultured HL-60 cells which are indicative of myeloid maturation. After 3 to 5 days of growth in the presence of L-ethionine, the majority of cells have enhanced phagocytic ability. The percentage of cells in the culture which bear complement receptors and which can respond to 12-O-tetradecanoylphorbol-13-acetate with respiratory burst activity increases more than 3-fold. Since the cells fail to become adherent and lose nonspecific esterase activity, we conclude that L-ethionine, like dimethyl sulfoxide, induces granulocytic differentiation of HL-60 cells.
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004950:Esterases / Q000032:analysis; D005001:Ethionine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006098:Granulocytes / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects; D006600:Hexosephosphates / Q000378:metabolism; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology; D007951:Leukemia, Myeloid / Q000473:pathology; D009195:Peroxidase / Q000032:analysis; D010587:Phagocytosis; D010703:Phorbol Esters / Q000494:pharmacology; D011289:Preleukemia / Q000473:pathology*; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D013997:Time Factors
D006600:Hexosephosphates; D010703:Phorbol Esters; D011951:Receptors, Complement; D011961:Receptors, Fc; D009195:Peroxidase; D004950:Esterases; D005947:Glucose; D005001:Ethionine; D004121:Dimethyl Sulfoxide