Cooperation of cytochalasin D and anti-microtubular agents in stimulating superoxide anion production in polymorphonuclear leukocytes.
Superoxide anion production in polymorphonuclear leukocytes obtained from guinea pig peritoneal cavities was stimulated by treatment with 5 microgram/ml of cytochalasin D in vitro. The stimulation was further enhanced by the addition of either 10(-5)-10(-7) M vinblastine or 10(-3)-10(-8) M colchicine to cytochalasin D. Higher concentrations of these anti-microtubular agents were inhibitory to the stimulating effect of cytochalasin D on the superoxide anion production, while lower concentrations had almost no effect. In the resting leukocytes, i.e. without cytochalasin D, the anti-microtubular agents by themselves had no effect on the superoxide anion production. The cooperation of cytochalasin D and the anti-microtubular agents was also observed in the stimulation of NADPH oxidase activity and bactericidal function of the leukocytes.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D004926:Escherichia coli; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000097:blood*; D010587:Phagocytosis; D013481:Superoxides / Q000097:blood*; D014747:Vinblastine / Q000494:pharmacology*
D003572:Cytochalasins; D013481:Superoxides; D015638:Cytochalasin D; D014747:Vinblastine; D010100:Oxygen; D003078:Colchicine