Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector.
We have constructed a simian virus 40 recombinant carrying a fragment of DNA from hepatitis B virus. Cultured monkey kidney cells infected with this recombinant produce hepatitis B surface antigen. The antigen is excreted into the culture medium as 22-nm particles with the same physical properties, antigenic composition, and constituent polypeptides as those found in the sera of patients with type B hepatitis.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D000956:Antigens, Viral / Q000235:genetics*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004274:DNA, Recombinant; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D006515:Hepatitis B virus / Q000276:immunology*; D007158:Immunologic Techniques; D013539:Simian virus 40 / Q000235:genetics
D000954:Antigens, Surface; D000956:Antigens, Viral; D004274:DNA, Recombinant