Organization of viral genome in a T antigen-negative hamster tumor induced by human papovavirus BK.
We analyzed by blot hybridization the state and structure of the viral DNA in an exceptional BK virus-induced hamster tumor (choroid plexus papilloma Vn-324) that contains about one copy of the BK virus genome per cell, but no intranuclear T antigen as assayed by indirect immunofluorescence. The BK viral DNA was found to be integrated into cellular DNA at a site in the middle of the early region of the viral genome (between 0.32 and 0.41 map units). The structure of the inserted viral DNA shows that it cannot encode a full-size large T antigen, but may encode small T antigen and an N-terminal portion of large T antigen.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D000957:Antigens, Viral, Tumor; D001739:BK Virus / Q000235:genetics*; D001483:Base Sequence; D002551:Cerebral Ventricle Neoplasms / Q000382:microbiology; D002831:Choroid Plexus; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000235:genetics*; D011995:Recombination, Genetic; D014412:Tumor Virus Infections / Q000382:microbiology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral