Sensitivity of viruses to phosphorylated 9-(2-hydroxyethoxymethyl)guanine revealed in TK-transformed cells.
Vaccinia and pseudorabies viruses are resistant to ACV [Acyclovir or 9-(2-hydroxyethoxymethyl)guanine] in normal cells. However, both viruses are sensitive in thymidine kinase (TK)-transformed cells in which the resident HSV-specific TK is able to phosphorylate the drug. This demonstrates the sensitivity of these viruses to phosphorylated ACV and suggests a wider antiviral activity for the phosphorylated drug.
D000212:Acyclovir / Q000031:analogs & derivatives; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006147:Guanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D011558:Herpesvirus 1, Suid / Q000187:drug effects*; D051379:Mice; D010766:Phosphorylation; D013937:Thymidine Kinase / Q000378:metabolism*; D014616:Vaccinia virus / Q000187:drug effects*; D010948:Viral Plaque Assay
D006147:Guanine; C025114:acyclovir triphosphate; D013937:Thymidine Kinase; D000212:Acyclovir