Introduction of active enzymes into intact Escherichia coli cells by means of liposomes. Phenotypic suppression of uvr A and pol A mutants.
Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons).
D002463:Cell Membrane Permeability; D004256:DNA Polymerase I / Q000008:administration & dosage*; D004259:DNA-Directed DNA Polymerase / Q000008:administration & dosage*; D004706:Endodeoxyribonucleases; D004720:Endonucleases / Q000008:administration & dosage*; D004926:Escherichia coli / Q000201:enzymology*; D008081:Liposomes; D009154:Mutation; D010641:Phenotype
D008081:Liposomes; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C026044:deoxyribonuclease V