Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B.
Clostridium perfringens strain ATCC 3626B was cured of caseinase activity at a high frequency after treatment with acriflavine dye (2.5%) or elevated temperature growth (9.1%). Caseinase-negative isolates retained the larger (9.4 megadaltons) pHB102 cryptic plasmid, but were missing the smaller (2.1 megadaltons) pHB101 plasmid present in the caseinase-positive wild-type strain. Dye-buoyant density-gradient centrifugation at 4 or 15 degrees C revealed that the pHB101 and pHB102 plasmids are temperature labile and easily converted into the nicked non-supercoiled or linear state.
D000167:Acriflavine / Q000494:pharmacology; D003016:Clostridium perfringens / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial; D004270:DNA, Circular; D008666:Metalloendopeptidases; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids
D004269:DNA, Bacterial; D004270:DNA, Circular; D000167:Acriflavine; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases; C030895:caseinase