title: Use of a continuous feline cell line for virologic and serologic investigations of bovine leukemia virus infections.
abstract: Simple, practical, reproductible methods for the detection of bovine leukemia virus (BLV) in peripheral blood leukocytes and BLV-neutralizing antibodies in bovine sera are described. Virus detection is based on syncytium formation in transformed feline (F-81) cells. The use of multiwell culture plates provides practical benefits in testing large numbers of samples. Results obtained in 2 farm herds show that the agreement of serologic and virologic test results may vary considerably from herd to herd.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002415:Cats; D002417:Cattle / Q000276:immunology; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D002460:Cell Line; D007938:Leukemia / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000254:growth & development* / Q000276:immunology; D008214:Lymphocytes / Q000382:microbiology; D009500:Neutralization Tests; D012190:Retroviridae / Q000254:growth & development*
chemical_list: D000914:Antibodies, Viral