Concentration and purification of poliovirus by immune adsorption on immobilized antibodies.
Antibodies to poliovirus type 1, 2 and 3 have been coupled to Sepharose 4 B. The immune sorbentia have high binding capacities for both live and inactivated virus. Bound antigen is eluted with a high concentration of ammoniumisothiocyanate. Immediately after elution the chaotropic salt is removed by gelfiltration. The antigen recovery with inactivated virus was low; however, with live virus a good recovery was obtained. Therefore, immune adsorption seems to be applicable for concentration and purification of large amounts of live poliovirus.
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007163:Immunosorbent Techniques; D008828:Microbiological Techniques; D017955:Poliovirus / Q000276:immunology / Q000302:isolation & purification*; D011054:Poliovirus Vaccine, Inactivated
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011054:Poliovirus Vaccine, Inactivated