Identification of deoxyribonucleic acid restriction fragments of beta-converting corynebacteriophages that carry the gene for diphtheria toxin.
Deoxyribonucleic acid fragments bearing the gene for diphtheria toxin have been identified in restriction enzyme digests of deoxyribonucleic acids from beta-converting and gamma-nonconverting corynebacteriophages. A combination of physical and genetic evidence has established that the Bam HI band C fragment of beta phage deoxyribonucleic acid, which carries the specific phage attachment site (Buck and Groman, J. Bacteriol. 148:131-142, 1981), also carries most, and probably all, of the gene for diphtheria toxin. A detailed restriction map of this tox-bearing Bam HI fragment has been developed, and the locations and orientation of the tox gene and the attP site within this fragment have been established.
D001287:Attachment Sites, Microbiological; D001435:Bacteriophages / Q000235:genetics*; D003353:Corynebacterium diphtheriae; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004167:Diphtheria Toxin / Q000235:genetics*; D005814:Genes, Viral
D004279:DNA, Viral; D004167:Diphtheria Toxin; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific