Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.
A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of the individual fragments from the paper with 1 M NaCl. DNA isolated from agarose gels in this way is susceptible to cleavage with several restriction endonucleases, and can be labeled in vitro with E coli DNA-polymerase I, T4 DNA-polymerase and T4 polynucleotide kinase. We have used the method to construct restriction endonuclease maps of adenovirus type 16 DNA.
D000256:Adenoviridae / Q000032:analysis*; D002854:Chromatography, Paper / Q000379:methods; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D008970:Molecular Weight
D004279:DNA, Viral; D004262:DNA Restriction Enzymes