Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).
A solid-phase approach using trinucleotide building blocks and functionalized cellulose as solid support was used to synthesize the 22 oligodeoxynucleotides that constitute a totally synthetic gene coding for a 43 amino acid peptide. Gastric inhibitory Polypeptide. The overall strategy used for the design and synthesis of the oligomers is described.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002482:Cellulose; D003062:Codon; D004262:DNA Restriction Enzymes; D005749:Gastric Inhibitory Polypeptide / Q000235:genetics*; D005768:Gastrointestinal Hormones / Q000235:genetics*; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*
D003062:Codon; D005768:Gastrointestinal Hormones; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D005749:Gastric Inhibitory Polypeptide; D002482:Cellulose; D004262:DNA Restriction Enzymes