Polyacrylamide gel electrophoresis of avian rotavirus RNA.
The RNAs of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. One of these isolates was made from chickens, the other three were from turkeys. The RNAs were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 X 10(6) to 2.09 X 10(6). In contrast to calf rotavirus, segments 10 and 11 of avian rotavirus RNA had very similar or identical electrophoretic mobilities. This may be a unique feature of avian rotavirus RNA. Co-electrophoresis experiments showed that the RNAs of 2 of the turkey isolates were electrophoretically identical. However, differences existed between these 2 isolates, the chicken isolate and the remaining turkey isolate with respect to segments 5, 6, the 7, 8, 9 complex and 10. Segment 5 of the chicken isolate was sometimes resolved into 2 components, and was further anomalous in that it was absent in some (analyses).
D000818:Animals; D002645:Chickens / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008970:Molecular Weight; D012328:RNA Viruses / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis; D012260:Ribonucleases / Q000494:pharmacology; D012401:Rotavirus / Q000032:analysis* / Q000235:genetics; D014422:Turkeys / Q000382:microbiology
D012367:RNA, Viral; D012260:Ribonucleases