Isolation and electron microscopic observations of intracytoplasmic inclusions containing Chlamydia psittaci.
Intracytoplasmic inclusions containing Chlamydia psittaci were isolated by a newly established method. Infected L-cells at 20 h after infection were suspended in 0.25 M sucrose-tris(hydroxymethyl)aminomethane buffer containing ethylene-diaminetetraacetic acid, homogenized in a Dounce tissue grinder, and filtered through a 2,000-mesh screen. Isolated inclusions were stabilized in 5% bovine serum albumin in 10 mM tris(hydroxymethyl)aminomethane buffer. Electron microscopic observations revealed the presence of surface projections on the vegetative, reticulate bodies and a direct connection between the reticulate bodies and the inclusion membrane by means of projections.
D000818:Animals; D002458:Cell Fractionation; D002691:Chlamydophila psittaci / Q000648:ultrastructure*; D002479:Inclusion Bodies / Q000382:microbiology* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000648:ultrastructure; D007739:L Cells; D051379:Mice; D008854:Microscopy, Electron