Protein-linked RNA of poliovirus is competent to form an initiation complex of translation in vitro.
Poliovirus RNA that had been labelled with 125I in the 5'-terminal protein (VPg) was found competent to form an initiation complex of translation in a cell-free reticulocyte lysate. In conditions of ribosome binding, no cleavage occurred between VPg and RNA. We conclude that removal of VPg from poliovirus RNA is not a prerequisite for this RNA to initiate translation in vitro.
D002474:Cell-Free System; D009698:Nucleoproteins / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational; D017955:Poliovirus / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D009698:Nucleoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins