Selective blotting of restriction DNA fragments on nitrocellulose membranes at low salt concentrations.
The pattern of restriction DNA fragments transferred from agarose gel to nitrocellulose membranes (Southern's technique) can be affected by the salt concentration of the transfer solvent.
D000327:Adsorption; D002951:Citrates; D003101:Collodion; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D010957:Plasmids; D012441:Saccharomyces cerevisiae; D012965:Sodium Chloride; D012997:Solvents
D002951:Citrates; D004274:DNA, Recombinant; D012997:Solvents; D012965:Sodium Chloride; D003101:Collodion; D004262:DNA Restriction Enzymes