A new method for the purification of DNA-binding proteins with sequence specificity.
We describe a technique for a rapid and efficient isolation and purification of proteins binding to defined DNA sequences. Cloned double-stranded DNA was covalently coupled to m-aminobenzyloximethylcellulose in order to purify proteins which recognize and bind to specific sequences on the DNA. The purification of two DNA-binding proteins from Drosophila melanogaster is demonstrated using the respective cloned DNA sequences.
D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000201:enzymology; D005260:Female; D010063:Ovum / Q000201:enzymology; D010957:Plasmids; D013379:Substrate Specificity
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D004265:DNA Helicases