Metabolic requirements for rabbit polymorphonuclear leucocyte lipoxygenase activity.
Lipoxygenase product formation by rabbit PMNs on exposure to the divalent ionophore A23187 was dependent upon pH, metabolic energy and substrate availability. Formation of 5-HETE and 5,12-diHETE (the major arachidonate oxygenation products) was inhibited by calcium depletion, by the metabolic inhibitors iodoacetate and 2-deoxyglucose, and by the substrate inhibitor TYA. The reaction had a pH optimum of 7.0-7.5. Colchicine, cytochalasin B and isoprenaline had no significant effects on HETE formation.
D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis; D000001:Calcimycin / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids; D007545:Isoproterenol / Q000494:pharmacology; D007975:Leukotriene B4; D008084:Lipoxygenase / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D011817:Rabbits; D013997:Time Factors
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D000001:Calcimycin; D003571:Cytochalasin B; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D008084:Lipoxygenase; D007545:Isoproterenol; D003078:Colchicine