Human papilloma virus type I purified from human genital warts.
Human wart virus (HPV) was isolated from a pool of genital warts. The electrophoretic mobility of virion proteins was studied by SDS polyacrylamide gel electrophoresis and showed the same pattern as that obtained with HPV-1. The analysis of DNA after restriction enzyme digestion with the endonucleases Hind III and Hae III, and nucleic acid hybridization did not show any difference with HPV-1. The viral particles were agglutinated by anti-HPV-1 serum, as shown by electron microscopic particle agglutination test. Furthermore, the immunological properties of this virus were investigated with guinea pig antiserum. Serologically, no antigenic cross-reaction between common and genital wart viruses were shown by immunodiffusion and immunofluorescence tests, whereas cross-reactions were detected between plantar and genital wart viruses. These results allow to think that HPV-1 can induce plantar warts as well as genital warts.
D000818:Animals; D003218:Condylomata Acuminata / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D005833:Genital Neoplasms, Female / Q000382:microbiology*; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification; D010502:Perineum / Q000382:microbiology; D014412:Tumor Virus Infections / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis; D014846:Vulvar Neoplasms / Q000382:microbiology
D004279:DNA, Viral; D014764:Viral Proteins