Elicitation of anti-viral cytotoxic T lymphocytes with purified viral and H-2 antigens.
The minimal molecular requirements for elicitation of secondary anti-Sendai virus CTL were investigated. The hemagglutinin-neuraminidase (HN) glycoprotein of Sendai virus and the H-2Kk glycoprotein of YAC tumor cells were purified and incorporated into phospholipid vesicles. These unilamellar liposomes were then tested for the ability to elicit H-2 restricted secondary anti-Sendai virus CTL. The results indicate that these well-defined vesicles were capable of eliciting secondary anti-Sendai virus CTL which lysed only target cells possessing the H-2Kk haplotype and modified with inactivated Sendai virus.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification*; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins; D006183:H-2 Antigens / Q000302:isolation & purification; D006388:Hemagglutinins; D008081:Liposomes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D006183:H-2 Antigens; D006388:Hemagglutinins; D008081:Liposomes; D009439:Neuraminidase