Odontoblast metabolism in rats deficient in vitamin D and calcium. IV. Lysosomal and energy metabolic enzymes.
Young rats, fed a low calcium and vitamin D deficient diet for 2 weeks, developed hypocalcemia, an increased activity of serum alkaline phosphatase and an increase in the serum concentration of immunoreactive parathyroid hormone. An increased activity of lactate dehydrogenase and cytochrome oxidase in odontoblasts was found. No shift in the general energy metabolic pathway was found as visualized in the lactate dehydrogenase iso-enzyme pattern. The dominating lactate dehydrogenase isoenzyme in odontoblasts from both the normal and the deficient rats was LDH 1 (H4, LD5), thus indicating primarily an aerobic energy-metabolism Also the activities of the lysosomal enzymes acid phosphatase, cathepsin D and hyaluronidase in the odontoblasts from the deficient animals were increased when compared to the normal animals. No significant change could be demonstrated for beta-glucuronidase and beta-N-acetylglucosaminidase. It was earlier found that this deficient diet caused an increase in odontoblast alkaline phosphatase activities and protein synthesis in vitro. In view of the present findings it might be concluded that the low calcium and vitamin D deficient diet causes a general increase in the odontoblast metabolism. It is not known whether this is due to the increase in parathyroid hormone or if it is a direct effect of the lowered serum calcium concentration.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D005966:Glucuronidase / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D006996:Hypocalcemia / Q000378:metabolism*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D009804:Odontoblasts / Q000378:metabolism*; D051381:Rats; D014808:Vitamin D Deficiency / Q000378:metabolism*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003576:Electron Transport Complex IV; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005966:Glucuronidase; D006821:Hyaluronoglucosaminidase; D000118:Acetylglucosaminidase; D002403:Cathepsins