title: The in vitro effects of Newcastle disease virus on the metabolic and antibacterial functions of human neutrophils.
abstract: Live Newcastle disease virus (NDV) was used to investigate the in vitro effects of a viral infection on phagocytosis, chemiluminescence generation, superoxide production, oxygen consumption, NADPH-oxidase activity, and intracellular killing of bacteria by Ficoll-Hypaque separated human neutrophils. Phagocytosis of oil red O particles by NDV-treated PMN was inhibited by 50%. Chemiluminescence by PMN was inhibited 79% after zymosan stimulation and 86% after tetradeconyl phorbol acetate stimulation. Superoxide generation was inhibited by 68%. Oxygen consumption was inhibited in the presence of NDV by 37% after stimulation with phorbol myristate acetate, while membrane-associated NADPH-enzyme activity was decreased by 19%. The percent of surviving intracellular S. aureus was significantly elevated in NDV-treated PMN after 60 and 120 min of incubation. Purified bacterial neuraminidase markedly suppressed chemiluminescence, while neuraminic acid blocked the effects of the virus. These observations suggest that infections with myxoviruses may suppress a number of vital neutrophil functions. It appears that the effects may be partly mediated by the interaction of viral neuraminidase with the external neutrophil membrane.
mesh_terms: D000328:Adult; D000818:Animals; D001717:Birds; D001770:Blood Bactericidal Activity; D006801:Humans; D008163:Luminescent Measurements; D008875:Middle Aged; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism* / Q000382:microbiology; D009521:Newcastle Disease / Q000378:metabolism* / Q000382:microbiology; D009522:Newcastle disease virus / Q000528:radiation effects; D009821:Oils; D010101:Oxygen Consumption; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D009821:Oils; D013481:Superoxides; D015054:Zymosan; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D009439:Neuraminidase; D013755:Tetradecanoylphorbol Acetate