Characterization of the beta-lactamase promoter of pBR322.
The beta-lactamase promoter of pBR322, derived from Tn3, has been characterized using several techniques. The transcription initiation site is located 35 base pairs from the translation initiation codon of beta-lactamase. The mRNA produced in vitro has a 5' pppGpA terminus. RNA polymerase bound at this start site protects a region from about -50 to +20 from DNase I cleavage using the footprinting technique. RNA polymerase binds rapidly to the beta-lactamase promoter. The half-time of association is less than one-half minute. The half-time of dissociation is approximately 6 hr. A study of the binding of RNA polymerase at different temperatures showed a large change between 11 degrees and 15 degrees C. Comparison of these parameters with those reported for other promoters is discussed.
D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases; D004720:Endonucleases; D004926:Escherichia coli / Q000201:enzymology; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics*
D003062:Codon; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D001618:beta-Lactamases