Microenzymatic assays for lysosomal enzymes in primary amniotic fluid cell cultures.
A study of three lysosomal enzymes (hexosaminidase, beta-galactosidase and alpha-galactosidase) in normal primary amniotic fluid cell cultures using a microenzymatic assay is presented. No difference in enzyme activity was found between primary and amniotic cell cultures in passage number one. A progressive change in the proportions of hexosaminidase A and hexosaminidase B with time was demonstrated in culture. The feasibility of this procedure for the early prenatal diagnosis of disorders due to lysosomal enzyme deficiency is discussed.
D000653:Amniotic Fluid / Q000166:cytology / Q000201:enzymology*; D002478:Cells, Cultured; D004796:Clinical Enzyme Tests / Q000379:methods*; D005260:Female; D005696:Galactosidases / Q000378:metabolism*; D005733:Gangliosidoses / Q000175:diagnosis; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis; D013997:Time Factors; D000519:alpha-Galactosidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
D005696:Galactosidases; D006596:Hexosaminidases; D000519:alpha-Galactosidase; D001616:beta-Galactosidase