The formation of sphingomyelin from phosphatidylcholine in plasma membrane preparations from mouse fibroblasts.
The enzymatic formation of radioactive sphingomyelin from [14C]choline-labeled phosphatidylcholine was demonstrated to reside exclusively in the plasma membrane fraction of mouse fibroblasts. This activity has several properties in common with the phosphatidylcholine ceramide phosphocholine transferase of mouse liver microsomes. The enzyme has little if any phospholipase C activity and isotope dilution experiments suggest that phosphatidylcholine is the substrate rather than it is converted to CDP choline, phosphocholine, free choline or glycerophosphocholine prior to the transfer reaction. The activity is stimulated by the addition of bovine serum albumin and MnCl2 to the incubation mixtures. The plasma membrane localization of the enzyme suggests that it may have a central role in the biosynthetic pathways for sphingomyelin in mouse fibroblasts.
D000818:Animals; D002413:Cations, Divalent; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002794:Choline / Q000494:pharmacology; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D013109:Sphingomyelins / Q000096:biosynthesis*
D002413:Cations, Divalent; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D002798:Diacylglycerol Cholinephosphotransferase; D002794:Choline