An improved method for the preparation of human fetal and adult hepatocytes.
A method to prepare isolated hepatocytes from the small amounts of liver or liver fragments obtained from the human fetus following therapeutic abortion in early pregnancy is described. The hepatocytes have been characterized by electron microscopy and by their ability to dealkylate the benzodiazepine drug prazepam. The preparation of hepatocytes from a liver sample obtained from a kidney transplant donor is described and discussed.
D003584:Cytological Techniques; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D003012:Microbial Collagenase / Q000378:metabolism; D011247:Pregnancy
D004533:Egtazic Acid; D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase