Use of specific lysine modifications to identify the site of reaction between cytochrome c and ferricyanide.
The site of the reaction between horse heart ferrocytochrome c and ferricyanide was investigated by measuring the reaction rate of cytochrome c derivatives specifically modified at single lysine residues to form trifluoroacetyl or trifluoromethylphenylcarbamyl amino groups. Cytochrome c derivatives singly modified at lysines 8, 13, 25, 27, 72, 79, and 87 surrounding the heme crevice had rate constants decreased from that of native cytochrome c by factors of 1.29, 2.03, 1.12, 1.35, 1.46, 1.29, and 1.19, respectively. Modification of a given lysine with the bulky trifluoromethylphenylcarbamyl group caused nearly the same decrease in reaction rate as modification with the trifluoroacetyl group, indicating that the effect was due to removal of an electrostatic interaction between the protonated lysine amino group and ferricyanide. Modification of lysines 22, 55, 99, and 100 at the right side, bottom, and back of cytochrome c had no effect on the reaction rate. These results indicate that the reaction site is located at the exposed edge of the heme and that the electrostatic interaction between ferricyanide and cytochrome c is dominated by the lysine amino groups surrounding the heme crevice, which include lysine 86, in addition to the ones listed above. We have used the specific lysine modification results to estimate the contribution of each lysine amino group to the electrostatic interaction and have developed a semiempirical relation for the total electrostatic interaction.
D000818:Animals; D001665:Binding Sites; D003574:Cytochrome c Group / Q000378:metabolism*; D005292:Ferricyanides; D006736:Horses; D007700:Kinetics; D008239:Lysine; D008958:Models, Molecular; D009206:Myocardium / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D014269:Trifluoroacetic Acid / Q000494:pharmacology
D003574:Cytochrome c Group; D005292:Ferricyanides; D014269:Trifluoroacetic Acid; D008239:Lysine