Endogenous synthesis of prostaglandins E1 and I2 in 3T3 fibroblasts transformed by polyoma virus. Effects on adenosine 3':5'-monophosphate production.
Prostaglandins (PG)E1, E2 and I2 were produced by polyoma virus transformed (py) 3T3 fibroblasts. The levels of PGE1, PGE2 and 6-keto-PGF1 alpha (degradation product of PGI2) were 22.7, 225 and 33.2 ng/ml medium, respectively, 72 h after medium change. The stimulatory potencies of exogenous PGE1, PGE2 and PGI2 on adenosine 3':5'-monophosphate (cyclic AMP) formation were similar. Therefore, the prostaglandin mediated increase in cyclic AMP levels observed during growth of these cells (Claesson, H.-E., Lindgren, J.A. and Hammarström, S. (1977) Eur. J. Biochem. 74, 13) is largely (greater than 80%) mediated by PGE2 and to lesser extents by PGE1 and PGI2.
D015126:8,11,14-Eicosatrienoic Acid / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D002472:Cell Transformation, Viral; D000242:Cyclic AMP / Q000096:biosynthesis*; D011464:Epoprostenol / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D011120:Polyomavirus; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis*
D001095:Arachidonic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D011464:Epoprostenol; D000242:Cyclic AMP; D015126:8,11,14-Eicosatrienoic Acid