title: Macrophage and lymphocyte-depleted thymus reticuloepithelial cell cultures: establishment and functional influence on T-lymphocyte maturation, C-type virus expression and lymphomatous transformation in vitro.
abstract: A method of cultivation has been developed that yields thymus reticuloepithelial (TRE) cell monolayers composed almost entirely of epithelial-like cells, with no detectable macrophages or lymphocytes. These cultures show the capacity to induce responsiveness to lectin mitogens in immature thymocytes. Such monolayers induce Thy-1 expression in nude mouse spleen and bone marrow cells. The functional macrophage-lymphocyte depleted TRE monolayers can be cryptically infected by radiation leukemia virus (RadLV). However, RadLV-infected TRE cells lack the capacity to induce neoplastic transformation in normal weanling and newborn thymocytes. Cultures prepared from C57BL/Ka mice treated with a leukemogenic course of irradiation produce a B-fibrotropic nonleukemogenic virus, and normal thymocytes cocultivated with these monolayers do not undergo in vitro transformation. It is concluded that TRE monolayers depleted of macrophages and lymphocytes: (1) exhibit the functional capacity to induce some of the properties of mature T cells; (2) are infectable by RadLV; (3) are one of the sites for expression of ecotropic virus after leukemogenic fractionated irradiation; and (4) are not capable of transforming normal thymocytes in vitro.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004847:Epithelial Cells; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000494:pharmacology; D012190:Retroviridae; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D010835:Phytohemagglutinins; D003208:Concanavalin A