The isolation and characterization of a clonally related series of murine retrovirus-infected mouse cells.
Starting with cloned NIH 3T3 mouse cells we have isolated a series of related lines infected with the Kirsten murine sarcoma/leukaemia (MSV/MLV) virus complex. These lines exhibit all three possible infected cell phenotopes: (i) transformed MSV/MLV producers; (ii) non-transformed MLV producers; (iii) transformed non-producers. We have also selected non-transformed revertants from one of the non-producer clones. This series of lines allows the study of the expression of the virus genome against a constant background of cellular gene expression. We have further characterized the lines with regard to anchorage dependence of growth, tumorigenicity and the presence of a rescuable sarcoma genome. The non-producer clones are uniform in their transformed properties. The revertants contain rescuable sarcoma virus, biologically indistinguishable from the original transforming virus, implying that the reversion is due to a change in cellular rather than viurs genetic information.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000382:microbiology; D005347:Fibroblasts; D007708:Kirsten murine sarcoma virus / Q000502:physiology*; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D051379:Mice; D009053:Sarcoma Viruses, Murine / Q000254:growth & development / Q000502:physiology*