Physical map of the conjugal plasmid of Neisseria gonorrhoeae.
The 24.5-megadalton plasmid of Neisseria gonorrhoeae is required for transfer of R-factors and possibly chromosomal markers during conjugal matings between gonococcal strains. We constructed a physical map of one such plasmid, pLE2451, using EcoRI, BglII, and HincII site-specific restriction endonucleases. The patterns of deoxyribonucleic acid digestion obtained with this plasmid were identical to those obtained with three other plasmids of similar size.
D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000032:analysis / Q000235:genetics*; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes