[Evidence, in vitro, of estrone and estradiol-17 beta 3-sulfoconjugate hydrolysis in the uterus of pregnant guinea pigs].
The metabolism, in vitro, of [6,7-3H]-oestrone-3-sulfate and [6,7-3H]-oestradiol-17beta-3-sulfate by the uteri of pregnant female Guinea-Pigs has been studied using 900 g supernatants. A hydrolysis of the sulfate moiety was observed: 35% of the radioactivity was recovered in the unconjugated fractions after 1 hr. of incubation. Gluruconide oestrogens were also recovered but the rate of synthesis was very low (3.6% after 1 hr.). OEstrone and oestradiol-17beta have been identified in unconjugated, glucuro and sulfo-conjugated fractions after incubation with the tritiated oestrogen sulfates.
D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000378:metabolism; D004966:Estrogens, Conjugated (USP) / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D006868:Hydrolysis; D007700:Kinetics; D011247:Pregnancy; D011270:Pregnancy, Animal; D014316:Tritium; D014599:Uterus / Q000378:metabolism*
D004966:Estrogens, Conjugated (USP); D014316:Tritium; D004970:Estrone; C028628:estradiol-3-sulfate; D004958:Estradiol; C017296:estrone sulfate
Mise en évidence, in vitro, de l'hydrolyse de l'oestrone et de l'oestradiol-17beta 3-sulfoconjugue's par l'utérus gravide de Cobaye.