Transcription and in vitro processing of yeast 5 S rRNA.
A method is described for the isolation of a yeast chromatin fraction highly enriched in ribosomal DNA sequences. In the presence of exogenous yeast RNA polymerase III, this purified chromatin actively synthesizes a set of 5 S ribosomal RNAs all of which have 5'-sequences identical with mature 5 S RNA but which end with a variable number (up to 10) of additional residues at the 3'-terminus. These extra nucleotides are precisely removed by a processing nuclease found in the chromatin supernatant fraction.
D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005815:Genetic Code; D007700:Kinetics; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000032:analysis; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D006163:Ribonuclease T1; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D006163:Ribonuclease T1