Epizootic hemorrhagic disease virus in Montana: isolation and serologic survey.
Epizootic hemorrhagic disease virus (EHDV) was isolated in Vero cell culture from the spleen and whole blood of a white-tailed deer (Odocoileus virginianus). A 10% spleen suspension caused acute hemorrhagic disease (HD) when inoculated into an experimental white-tailed deer and resulted in the recovery of EHDV from the blood of the experimental animal at 5 days after inoculation. The virus was identified as EHDV serotype 2 through indirect fluorescent antibody tests, electron microscopy, and reciprocal cross-neutralization tests. Approximately 73% (36/49) of the mule deer, 5% (2/42) of the white-tailed deer, and 79% (249/314) of the cattle samples tested from areas where HD had been reported were EHDV seropositive. Although none of the white-tailed deer was bluetongue virus seropositive, 29% of the mule deer and 3% of the cattle tested from "active" HD areas possessed bluetongue virus precipitating antibody.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001769:Blood / Q000382:microbiology; D002417:Cattle / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003670:Deer / Q000276:immunology; D007668:Kidney; D009009:Montana; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D014776:Virus Cultivation
D000914:Antibodies, Viral