title: Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon.
abstract: The nucleotide sequence of the structural gene coding for the respiratory NADH dehydrogenase of Escherichia coli has been determined by the chain-termination method. The reading frame for the protein starts with the unusual initiation codon UUG and predicts an amino acid sequence of 434 residues (Mr = 47 304). The reading frame was confirmed by protein chemical studies including determination of the N-terminal sequence of the protein. The product made in vivo was found to have threonine as its N-terminal residue, indicating that the initiating N-formylmethionine had been removed by post-translational processing.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D003579:Cytochrome Reductases / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D005815:Genetic Code; D009245:NADH Dehydrogenase / Q000235:genetics*; D012333:RNA, Messenger
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D012333:RNA, Messenger; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D004262:DNA Restriction Enzymes