An erythropoietic stimulating factor similar to erythropoietin released by macrophages after treatment with silica.
An erythropoietic stimulating factor (ESF) can be detected in the supernatant from fetal liver and adult bone marrow and spleen cells when preincubated with the macrophage-specific cytotoxic agent, silica. Stimulation is observed in 12-day fetal liver CFU-E cultures in the absence of added erythropoietin (Ep). The concentration of ESF in the supernatant added to CFU-E cultures is dependent on the preincubated cell dose and the volume added. The stimulating activity is abolished when mice are hypertransfused and increased above normal values when mice are bled. A concentrated silica-treated spleen supernatant was able to stimulate erythropoiesis in the polycythemic mouse bioassay. It is concluded that the ESF is similar, if not identical, to Ep.
D000740:Anemia / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D004921:Erythropoietin / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D011086:Polycythemia / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology*; D013154:Spleen / Q000378:metabolism; D013268:Stimulation, Chemical
D004921:Erythropoietin; D012822:Silicon Dioxide