Separation of human blood monocytes and lymphocytes on a continuous Percoll gradient.
A method for separation of human blood monocytes and lymphocytes is described. Mononuclear leukocytes are centrifuged on a continuous gradient of colloidal silica particles (Percoll) in phosphate-buffered saline. This leads to formation of 4 bands: a layer containing dead material (if present) which did not enter the gradient; a layer near the bottom of the tube containing granulocytes and red cells, and two other bands in between. The upper one is enriched for monocytes (av. 78%) and the lower one for lymphocytes (av. 98%). The final yields of these cell types are 73% and 79%, respectively, and their viability is greater than 95%. No functional impairments could be detected by different criteria including the ability of B lymphocytes to produce immunoglobulins when stimulated with pokeweed mitogen and the ability of monocytes to phagocytize opsonized red cells and latex particles.
D000921:Antibody-Producing Cells / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003102:Colloids; D006801:Humans; D008214:Lymphocytes; D008264:Macrophages; D009000:Monocytes; D012822:Silicon Dioxide
D003102:Colloids; D012822:Silicon Dioxide