title: Macrophages and the synthesis of connective tissue components.
abstract: 1. Macrophage homogenate, added to the incubation medium of granulation tissue slices, decreases the incorporation of labelled proline to collagen and other proteins, to a greater extent in the presence of certain prostaglandins and in oxygen atmosphere, but to a lesser degree if serotonin and cyclic AMP are added. 2. Rheumatoid synovial fluid, connective tissue active peptide (CTAP) of Castor and an extract from CCl4-damaged liver stimulate collagen synthesis in the presence of macrophages. 3. Preparations from non-treated macrophages stimulate the incorporation of glucosamine to acid mucopolysaccharides, mainly hyaluronate. 4. In a developing rat granuloma, the formation of collagen was stimulated by homogenized macrophages and by macrophage culture medium, but especially by whole macrophages in the presence of SiO2.
mesh_terms: D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D003238:Connective Tissue; D000242:Cyclic AMP / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006099:Granuloma / Q000378:metabolism*; D008110:Liver Extracts / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D013582:Synovial Fluid
chemical_list: D006025:Glycosaminoglycans; D008110:Liver Extracts; D010455:Peptides; D011453:Prostaglandins; D012701:Serotonin; D003094:Collagen; D000242:Cyclic AMP; D010100:Oxygen