Preparative isoelectric focusing of poliovirus polypeptides in urea-sucrose gradients.
Isoelectric focusing in urea using small density gradient columns for dissociated poliovirus resulted in a complete separation of the four virus polypeptides. Identification and purity of VP1, 2, 3 and 4 was shown by SDS-polyacrylamide gel electrophoresis. The isoelectric points were determined and compared with the values obtained in gels. The recovery of the individual polypeptides was about 80%. Up to 500 microgram of poliovirus per tube could thus be separated in a one-step procedure giving pure and SDS-free poliovirus polypeptides.
D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D017955:Poliovirus / Q000032:analysis*; D013395:Sucrose; D014508:Urea; D014764:Viral Proteins / Q000302:isolation & purification*
D014764:Viral Proteins; D013395:Sucrose; D014508:Urea