[Method of detecting alkaline phosphatase and 5-nucleotidase in histological bone sections].
Modification of the methods of Gomory, Pearce, and Reis for the detection of alkaline phosphatase and 5-nucleotidase in the bone tissue is described. The authors used fixation of the bone tissue in acetone at a temperature of 0 to 4 degrees C for 6 to 12 hours, decalcification in Grip's fluid followed by cutting in a freezing microtome of incompletely decalcified bone material and its decalcification in sections. As a result, localization of the enzyme is detected by the distribution of black sediments of cobalt sulfide.
D015720:5'-Nucleotidase; D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D001842:Bone and Bones / Q000201:enzymology*; D006651:Histocytochemistry; D006652:Histological Techniques; D006801:Humans; D009708:Nucleotidases / Q000032:analysis*
D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase
K metodike vyiavleniia shchelochnoĭ fosfatazy i 5-nukleotidazy v gistologicheskikh srezakh kostnoĭ tkani.