Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.
pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D010957:Plasmids; D011995:Recombination, Genetic