title: Stimulation of herpes simplex type I infection of C6 cells by trypsin-EDTA.
abstract: The nature of the refractoriness of C6 glioblastoma cells to herpes simplex virus type I (HSV-I) infection has been studied. The cells were restricted in susceptibility to HSV-I since only a small proportion of the cells could be infected by HSV-I and the virus yield per cell was low. The susceptibility to infection was increased by treating the cells with trypsin-EDTA prior to infection. The cells so treated recovered resistance to the virus when incubated at 37 degrees C, their resistance being restored to the initial level in 2 days. This restoration was inhibited by addition of cycloheximide or puromycin. Trypsin-EDTA treatment of C6 cells increased the efficiency of adsorption of HSV-I and the formation of stable cell-virus complexes from which the virus could not be dissociated by heparin.
mesh_terms: D000327:Adsorption; D000375:Aging; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D004492:Edetic Acid / Q000494:pharmacology*; D005910:Glioma; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D014176:Protein Biosynthesis; D051381:Rats; D018139:Simplexvirus / Q000254:growth & development* / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D006493:Heparin; D004492:Edetic Acid; D014357:Trypsin