Potency test of killed polio vaccines. Statistical analysis of test on chicken and preliminary results in combination with ELISA D-antigen determinations.
The authors resume a several years' experience of potency testing inactivated polio vaccines with an in vivo-vitro assay based on inoculation of 3 week-old chicken and measure of neutralizing antibodies at 5 days on Hep 2 cells in a microplate system. Critical points, specificity and reproducibility are successively outlined. Preliminary combining results with determination of D-antigen by ELISA method and human immunization are submitted in view of a future standardization.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage / Q000592:standards*
D000956:Antigens, Viral; D011054:Poliovirus Vaccine, Inactivated