Mitochondrial heterogeneity in Aspergillus nidulans: in vivo protein biosynthetic activities of the mitochondrial populations.
Two mitochondrial populations with cytochrome c oxidase activity, and densities of 1.07 and 1.08 g/ml have been separated by means of a dextran-sucrose gradient. The highest enzymatic specific activity appeared in the 1.07 g/ml band. The mitochondria assayed by their in vivo cycloheximide resistant leucine incorporation appeared at the 1.08 g/ml density band of the gradient. Some amount of activity of cytochrome c oxidase, and leucine incorporation appeared at the bottom of the gradient. The cytochrome spectra in liquid nitrogen of each band showed different proportion of cytochrome(s) a, b and c. The highest ratio of cytochrome a and cytochrome b in respect to cytochrome c appeared in the 1.08 g/ml band.
D001233:Aspergillus nidulans / Q000166:cytology*; D001693:Biological Transport, Active; D003513:Cycloheximide / Q000494:pharmacology; D003580:Cytochromes / Q000032:analysis; D003576:Electron Transport Complex IV / Q000032:analysis; D007930:Leucine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014176:Protein Biosynthesis
D003580:Cytochromes; D003513:Cycloheximide; D003576:Electron Transport Complex IV; D007930:Leucine