[Effects of sulfomethylation on the mechanism of action of colimycin towards "Escherichia coli" B (author's transl)].
Colimycin is sulfomethylated in order to inactivate it temporarily in vivo. In vitro, this process may have unexpected results because the experiment is carried out within a closed system. The time required to activate the antibiotic varies according to incubation conditions. The freed sulfomethyl groups are probably eliminated in vivo. In vitro, these residues may interact with the outer membrane of Escherichia coli B which is charged with badly positioned molecules of colimycin, thus creating a barrier for the active molecules. As the expected mechanism of polymyxin B or E action include first electrostatic interactions, it is not surprising that a modification of the ionic balance of the medium should be of such importance.
D002462:Cell Membrane / Q000187:drug effects; D003091:Colistin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000187:drug effects; D008698:Mesylates; D008928:Mitochondria / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D008698:Mesylates; D003091:Colistin
Conséquences de la sulfométhylation sur le mode d'action de la colimycine chez Escherichia coli B.