title: Reexamination of the genome size of myxobacteria, including the use of a new method for genome size analysis.
abstract: The genome sizes of two myxobacteria, Myxococcus xanthus and Stigmatella aurantiaca, were measured by renaturation analysis and also by a new method involving the quantitation of individual restriction fragments. In contrast to several previous reports, which indicate that M. xanthus has a genome size which is three to four times that of Escherichia coli, the present measurements indicated that the M. xanthus genome is only about 24 to 53% larger than that of E. coli. S. aurantiaca had a genome size nearly identical to that of M. xanthus. Of possible significance is the fact that the renaturation curves of M. xanthus and S. aurantiaca deoxyribonucleic acid both exhibited significant fractions which renatured with rapid, unimolecular kinetics. However, we were unable to establish that these fractions represented inverted repeats of repetitive sequences.
mesh_terms: D002876:Chromosomes, Bacterial / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis; D009231:Myxococcales / Q000032:analysis*; D009695:Nucleic Acid Renaturation; D011550:Pseudomonas aeruginosa / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes