Esterification of cholesterol and lipid-soluble vitamins by human pancreatic carboxyl ester hydrolase.
Human pancreatic carboxyl ester hydrolase is shown to catalyse the esterification of cholesterol and lipid-soluble vitamins A, E and D3 with oleic acid. The acitivity requires the presence of bile salts, and the trihydroxylated or the 3 alpha, 7 alpha dihydroxylated bile salts are better activators than the 3 alpha, 12 alpha dihydroxylated bile salts. The hydrolyzing and synthetizing activities of human pancreatic carboxyl ester hydrolase are separated by a large pH range since the synthesis of cholesterol esters is optimal at pH 5.25 and the hydrolysis of cholesterol and vitamin E esters is optimal at pH 8.0. From the comparison of the catalytic constants determined for the hydrolyzing and synthetizing activities and from the pH dependence of the two activities, it appears that human carboxyl ester hydrolase plays an important part in the intestinal lumen. The role of the enzyme in the esterification of cholesterol and lipid-soluble vitamins is questionable.
D001647:Bile Acids and Salts / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002762:Cholecalciferol / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D009829:Oleic Acids; D010179:Pancreas / Q000201:enzymology*; D014801:Vitamin A / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism
D001647:Bile Acids and Salts; D002788:Cholesterol Esters; D009829:Oleic Acids; D014801:Vitamin A; D014810:Vitamin E; D002762:Cholecalciferol; D002784:Cholesterol; D002265:Carboxylic Ester Hydrolases