EBV-transformation of surface IgA-positive human lymphocytes.
Surface IgA-positive human lymphocytes were selected, separated, and immortalized with Epstein-Barr virus (EBV). Selection was carried out by rosetting the cells with ox erythrocytes, coated with rabbit anti-human IgA immunoglobulin, followed by separation on Ficoll-Isopaque. Stable monoclonal lines were established by cloning in agarose. This shows that EBV-transformation is not restricted to the IgM-positive B cell category but can be extended to minority classes of B lymphocytes. The method provides the means to establish other minority categories as continuous lines, depending on the efficiency of the preselection procedure.
D000818:Animals; D002417:Cattle; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D008214:Lymphocytes / Q000276:immunology*; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D011947:Receptors, Antigen, B-Cell