Mast cell restoration. A study of the rat peritoneal mast cells after depletion with polymyxin B.
The recovery of mast cells in adult rats after depletion induced by polymyxin-B has been studied. At days 17-18 after depletion the number of identifiable mast cells in the peritoneal fluid reached 3 X 10(6) cells/rat. Mast cell granule constituents such as histamine, heparin, mast cell protease and beta-glucuronidase increased steadily during the observation period, whereas the amount of N-acetyl-beta-glucosaminidase decreased during the first 2 weeks and was thereafter constant. At 2 weeks after depletion the peritoneal mast cells were biochemically restored. From 14 to 34 days (end of experimental period) cell growth occurred with a simultaneous accumulation of the various mast cell granule constituents.
D000118:Acetylglucosaminidase; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002452:Cell Count; D002469:Cell Separation; D005966:Glucuronidase; D006493:Heparin; D006632:Histamine; D008297:Male; D008407:Mast Cells / Q000648:ultrastructure; D010447:Peptide Hydrolases; D011112:Polymyxin B / Q000494:pharmacology*; D011113:Polymyxins / Q000494:pharmacology*; D051381:Rats
D011113:Polymyxins; D006632:Histamine; D006493:Heparin; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D010447:Peptide Hydrolases; D011112:Polymyxin B