The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.
An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments.
D010582:Bacteriophage lambda; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology*; D004279:DNA, Viral; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000302:isolation & purification*; D009838:Oligodeoxyribonucleotides / Q000032:analysis
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004720:Endonucleases