title: Lipoxygenation of arachidonic acid as a source of polymorphonuclear leukocyte chemotactic factors in synovial fluid and tissue in rheumatoid arthritis and spondyloarthritis.
abstract: The predominant lipoxygenase products of arachidonic acid were extracted and purified from synovial fluid and sonicates of synovial tissue of patients with rheumatoid arthritis (RA), spondyloarthritis (SA), or a noninflammatory arthropathy (NIA). The concentration of 5(S),12(R)-dihydroxy-6,8,10-(trans/trans/cis)-14-cis-eicosatetraenoic acid (leukotriene B4) in synovial fluid was elevated significantly in patients with RA and a positive latex test for rheumatoid factor (P < 0.05, n = 14) and in patients with SA (P < 0.05, n = 10), compared with that of subjects with NIA (n = 9). The content of 5(S)-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), but not of leukotriene B4, was elevated significantly in synovial tissue of seven patients with RA in comparison with that of four subjects with NIA (P < 0.05). A single intra-articular injection of corticosteroid significantly lowered the synovial fluid level of leukotriene B4 in six patients with RA. These data suggest an involvement of the potent chemotactic factors 5-HETE and leukotriene B4 in human inflammatory disease.
mesh_terms: D001095:Arachidonic Acids / Q000032:analysis* / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000097:blood*; D002630:Chemotactic Factors / Q000097:blood*; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D008084:Lipoxygenase / Q000378:metabolism; D009504:Neutrophils / Q000503:physiopathology*; D013167:Spondylitis, Ankylosing / Q000097:blood*; D013582:Synovial Fluid / Q000032:analysis
chemical_list: D001095:Arachidonic Acids; D002630:Chemotactic Factors; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D008084:Lipoxygenase