Hybrid plasmids carrying part of the Rous sarcoma virus-specific leader sequence.
'Strong-stop' DNA, complementary to the 5'-terminal 101 nucleotides of Rous sarcoma virus genomic RNA and subgenomic mRNAs can be used to isolate RSV-specific mRNA from infected cells. To analyse leader sequences of these mRNAs and to study the mechanism of their translation, relatively large quantities of strong-stop DNA are required. The construction of four plasmids carrying useful strong-stop DNA sequences for mRNA isolation is described. One such hybrid plasmid carries a DNA insertion containing sequences derived from a region starting 42 nucleotides from the 5'-end of RSV RNA, and ending at the tRNATry primer attachment site. Since this plasmid lacks the 20-nucleotide terminal repeat sequence it can be used for the specific isolation of 5'-end fragments of RSV-specific mRNAs and of complementary DNA transcripts of these fragments.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D004262:DNA Restriction Enzymes