A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2.
In this paper we describe the synthesis and cloning of a short segment of DNA complementary to the region immediately adjacent to the EcoRI insertion site in the single-stranded bacteriophage vector M13mp2. This segment is useful as a "universal" primer for DNA sequencing by the dideoxynucleotide chain termination method; the template can be any DNA species cloned in M13mp2 or its derivatives. The primer has been cloned into the tetracycline resistance gene of plasmid pBR322 as one strand of a 26 bp EcoRI/BamHI fragment. This fragment may be readily prepared from an EcoRI + BamHI restriction digest of the parent plasmid (designated pSP14) by a simple size fractionation.
D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D010957:Plasmids
D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes