title: Antigens which detect IgE antibodies in workers sensitive to toluene diisocyanate.
abstract: Two antigens, both containing tolyl groups, were compared for ability to detect IgE antibodies in workers hypersensitive to toluene diisocyanate. One antigen, formed by reaction of p-tolyl isocyanate with human serum albumin, detected antibodies in each of ten hypersensitive workers. A second tolyl antigen, formed by reaction of p-toluoyl chloride and human serum albumin, and therefore lacking isocyanate linkages, detected antibodies in seven of the ten workers. In RAST assays, sera from toluene diisocyanate-sensitive workers demonstrated higher binding to human serum albumin-coated control discs than did sera from non-sensitive workers. The significance of this binding in calculating tolyl-reactive antibody titres is discussed.
mesh_terms: D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D003485:Cyanates / Q000009:adverse effects*; D006801:Humans; D007073:Immunoglobulin E; D011852:Radioallergosorbent Test; D012130:Respiratory Hypersensitivity / Q000139:chemically induced*; D012709:Serum Albumin / Q000276:immunology; D014050:Toluene / Q000031:analogs & derivatives / Q000276:immunology; D014051:Toluene 2,4-Diisocyanate / Q000009:adverse effects*
chemical_list: D003485:Cyanates; D012709:Serum Albumin; D014051:Toluene 2,4-Diisocyanate; D007073:Immunoglobulin E; D014050:Toluene