The 5'-nucleotidase activity in normal human serum. Electrophoretic patterns and substrate specificity.
Both the electrophoretic patterns and substrate specificity of the total 5'-nucleotidase activity at pH 7.4 in serum from 10 normal adults (19-49 years) were identical using various purine and pyrimidine mononucleotides as substrates. Different purified alkaline phosphatases were studied at the same time in the same manner. The serum enzymes showed substrate specificity qualitatively similar to that of the bovine liver enzyme. The electrophoretic study showed two fractions for both the liver enzyme and the serum enzymes with the different substrates, indicating that each of these are dephosphorylated by the same two, mutually different, enzyme molecules. Each of the other enzymes showed only a single fraction, which was identical for each enzyme with the various substrates.
D015720:5'-Nucleotidase; D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009708:Nucleotidases / Q000097:blood* / Q000302:isolation & purification; D013379:Substrate Specificity
D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase