Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.
The structural genes for translational initiation factor IF3, threonyl-tRNA synthetase (TRS), the two subunits of phenylalanyl-tRNA synthetase (PRS), and a 12 000 mol. wt. protein of unidentified function are carried by the lambda p2 transducing phage. The localization of these genes on a restriction map of the Escherichia coli DNA insert was achieved by deletion mapping. In addition a set of plasmids carrying fragments of the original phage was constructed and helped to confirm these assignments. One plasmid, containing a 3.3 kb PstI fragment inserted into pBR322, does not code for any of the synthetase genes but causes strains carrying it to overproduce IF3.
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D014176:Protein Biosynthesis; D012808:Sigma Factor / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D012808:Sigma Factor; D014157:Transcription Factors; D004262:DNA Restriction Enzymes