Properties of adeno-associated virus (type 1) replicated in rodent cells by murine adenovirus.
We report for the first time the replication of infectious adeno-associated virus type 1 (AAV-1) in rodent cells [primary mouse kidney (PMK) and mouse L929 cells] using murine adenovirus (MAV) as a helper virus and also the production of AAV-I virus antigen by herpes simplex virus type I (HSV-I) with its temperature-sensitive mutant ts 200 in mouse neuroblastoma (NB) cells. The infectious AAV virions produced by MAV on L cells had a buoyant density of 1.41 2ml in caesium chloride gradients.
D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D000956:Antigens, Viral; D002478:Cells, Cultured; D000229:Dependovirus / Q000254:growth & development* / Q000276:immunology; D006378:Helper Viruses / Q000502:physiology*; D007739:L Cells; D051379:Mice; D018139:Simplexvirus / Q000502:physiology; D014779:Virus Replication
D000956:Antigens, Viral