Carbon tetrachloride-induced loss of microsomal glucose 6-phosphatase and cytochrome P-450 in vitro.
Rat liver microsomes were incubated in the presence of NADPH and CCl4 under various conditions, and losses of glucose 6-phosphatase (G-6-Pase) and cytochrome P-450 were examined in terms of lipid peroxidation and CCl4 metabolism. Loss of G-6-Pase activity correlated well with the enhancement of lipid peroxidation. Loss of cytochrome P-450 was also dependent on the lipid peroxidation, under aerobic conditions. However, the cytochrome was destroyed under anaerobic conditions in which lipid peroxidation and loss of G-6-Pase were greatly suppressed. This anaerobic loss of cytochrome P-450 may be linked with the metabolism of CCl4 by this hemoprotein, as evidenced by the observation that CCl4 metabolism occurred only under anaerobic conditions and was inhibited by carbon monoxide accompanied by the suppression of the loss of cytochrome P-450.
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000378:metabolism / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats
D008054:Lipid Peroxides; D008315:Malondialdehyde; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D005952:Glucose-6-Phosphatase