Effect of DNA sequences adjacent to the termini of Tn3 on sequential translocation.
Insertion of Tn3 generates a five base pair repeat of a nucleotide sequence indigenous to the recipient genome. Tn3 promoted deletions extend precisely from the Tn3 terminus and remove one of the 5 base pair repeats while not affecting the ability of Tn3 to subsequently undergo translocation. A direct repeat of a 10 bp sequence located in the Tn3 termini occurs internally within Tn3 and may affect the orientation of insertion.
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002872:Chromosome Deletion; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D011995:Recombination, Genetic; D014178:Translocation, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes