Uptake of plasmid deoxyribonucleic acid by Haemophilus.
The uptake of circular and linear plasmid RSF0885 deoxyribonucleic acids, (DNAs) obtained from Haemophilus parainfluenzae 14, in both homologous and heterologous recipients was studied and compared with that of chromosomal DNA. High concentrations of divalent cations stimulated the uptake of either circular or linear plasmid DNA in H. parainfluenzae 14 competent cells but did not affect the uptake of chromosomal DNA. The biological activity of linear plasmid DNA was similar to that of circular DNA, and the transforming efficiencies for ampicillin resistance of both molecular forms were stimulated by divalent ions. Plasmid DNA was taken up efficiently either with or without the addition of divalent ions but was not biologically active in the heterologous Haemophilus influenzae Rd recipient. Our results suggest that in H. parainfluenzae 14 some of the steps for chromosomal and plasmid DNA uptake are different.
D002122:Calcium Chloride / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000187:drug effects*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism*; D004274:DNA, Recombinant; D006190:Haemophilus / Q000235:genetics*; D008278:Magnesium Sulfate / Q000494:pharmacology; D010957:Plasmids / Q000187:drug effects*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D008278:Magnesium Sulfate; D004262:DNA Restriction Enzymes; D002122:Calcium Chloride