Synthesis of thymidine kinase (TK) in Epstein-Barr virus-superinfected Raji TK-negative cells.
Epstein-Barr virus (EBV) from P3HR-1 cells, but not from B95-8 cells, can induce the synthesis of thymidine kinase (TK) in TK-negative Raji cells. The synthesis of TK was slightly reduced, but not inhibited, when cells were cultivated in the presence of cytosine arabinoside (ara-C). On the other hand, the synthesis of TK in ordinary Raji cells was enhanced in the presence of the drug. Thymidine-beta-D-arabinofuranoside (ara-T) was capable of reducing the conversion rate of thymidine to TdR nucleotides by extracts prepared from superinfected Raji TK-cells, but had no influence on TK activity in cell extracts from ordinary Raji cells and EBV-negative Ramos cells. This suggested a broader substrate specificity of the virally induced enzyme.
D001087:Arabinonucleosides / Q000494:pharmacology; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology; D004790:Enzyme Induction; D004854:Herpesvirus 4, Human / Q000201:enzymology* / Q000254:growth & development; D006801:Humans; D013379:Substrate Specificity; D013936:Thymidine / Q000031:analogs & derivatives / Q000494:pharmacology; D013937:Thymidine Kinase / Q000096:biosynthesis*
D001087:Arabinonucleosides; D003561:Cytarabine; D013937:Thymidine Kinase; C010032:thymine arabinoside; D013936:Thymidine