A terminal labelling assay using 3H-uridine to measure cell-mediated cytotoxic responses against parainfluenza type 3 infected target cells.
The standardisation of a terminal labelling cytotoxicity assay which used the incorporation of 3H-uridine to measure target cell survival of the effector phase is described. This assay was applied to a preliminary study of the cell-mediated responses of sheep following intravenous inoculation of Pi-3 virus. The assay provided a simple, accurate, and reproducible method to measure cytotoxic responses against Pi-3 infected target cells. The experimental data did not require mathematical corrections for non-specific effects as in the 51Cr-release assay. When cytotoxicity could be detected in uninfected target cell cultures it was low and statistically insignificant.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development* / Q000276:immunology; D018108:Respirovirus / Q000254:growth & development*; D012756:Sheep; D014529:Uridine / Q000378:metabolism
D014529:Uridine