Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine.
The Abelson murine leukaemia virus protein (P120) can become phosphorylated in vitro by [gamma-32P]ATP. The protein has been purified from cell membranes to the point that in specific conditions virtually all of the incorporated 32P is in P120. The reaction is stimulated by Mn2+ and Mg2+ but not Ca2+ and is very rapid even at 0 degrees C. The phosphate is linked to P120 at tyrosine, a linkage not previously reported for a phosphorylation reaction. Phosphorylation may be involved in the transforming activity of viruses that cause leukaemia as well as sarcomas.
D000011:Abelson murine leukemia virus / Q000378:metabolism*; D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses / Q000378:metabolism; D002472:Cell Transformation, Viral; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives; D014764:Viral Proteins / Q000378:metabolism*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D008565:Membrane Proteins; D014764:Viral Proteins; D014443:Tyrosine; D011494:Protein Kinases