[Changes in prostaglandin systhetase activity in mouse tissues as affected by S-[N-(3-aminopropyl)-2-aminoethyl] thiophosphoric acid].
Effect of various concentrations of a radioprotector S-[N-(3 aminopropyl)-2-aminoethyl] thiophosphoric acid on the activity of prostaglandine synthetase was studied in mouse liver microsomes as well as in the soluble fractions of testicules and brain in vitro. The activity of prostaglandine synthetase was estimated by monitoring the formation of labelled PGF2 alpha and PGE2 from I-14C-arachidonic acid. The radioprotector at concentration 1.66 mg/ml stimulated formation of PGF2 alpha in all the tissues studied. At the lower concentrations of the radioprotector only slight stimulation of the biosynthesis of prostaglandines in testicules was noted. No effect on their synthesis in the brain soluble fraction could be observed while in the liver microsomes it was inhibited. The radioprotective substance studied apparently affected the cyclooxygenase activity, which is a key enzyme in the prostaglandine-synthesizing system.
D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008862:Microsomes, Liver / Q000201:enzymology; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013737:Testis / Q000201:enzymology
D009946:Organothiophosphorus Compounds; D011837:Radiation-Protective Agents; D011451:Prostaglandin-Endoperoxide Synthases; D004999:Amifostine
Izmenenie aktivnosti prostaglandinsintetazy v tkaniakh mysheĭ pod vliianiem S-[N-(3-aminopropil)-2-aminoétil] tiofosfornoĭ kisloty.