title: Glucose oxidation in the ventromedial hypothalamus is not affected by insulin or ouabain but depressed by alloxan treatment.
abstract: Insulin is apparently not required for VMH glucose oxidation in vitro. Ouabain, an inhibitor of the Na-K pump ATPase, does not prevent VMH glucose oxidation in vitro. These data suggest (a) the VMH does not exhibit a cotransport phenomenon of glucose with the Na-K pump mechanism, and (b) glucose oxidation in the VMH is not insulin dependent. Alloxan-diabetes was induced to increase tissue insulin sensitivity. A comparison of glucose oxidation rates in alloxan-diabetic VMH tissue and normal VMH tissue, supplemented only with saline, indicated a highly significant (p < 0.001) depression of glucose oxidation in the alloxan-treated tissue. Cell membranes in the VMH are perhaps altered by alloxan.
mesh_terms: D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000187:drug effects*; D007033:Hypothalamus, Middle / Q000187:drug effects* / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects; D051379:Mice; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D007328:Insulin; D007473:Ion Channels; D010042:Ouabain; D000496:Alloxan; D012964:Sodium; D011188:Potassium