Test protocol of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus.
In this communication the test procedure is described for an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus (BLV). Test sera are incubated in polystyrene microtiter plates sensitized with a partly-purified preparation of BLV. Bovine antibodies are detected with anti-species immunoglobulin conjugated to the enzyme horseradish peroxidase, followed by the addition of the enzyme substrate.
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*
D000914:Antibodies, Viral