title: A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.
abstract: We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that a T to A transcription at the same nucleotide also drastically decreases transcription, suggesting that these mutations influence transcription mainly by altering nucleotide recognition rather than helix stability. Small deletions of 2, 3 and 4 nucleotides in the "TATA" box also drastically decrease specific initiation of transcription.
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003207:Conalbumin / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004527:Egg Proteins / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D012319:RNA Polymerase II / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D004527:Egg Proteins; D003207:Conalbumin; D012319:RNA Polymerase II; D004262:DNA Restriction Enzymes