The isolation and characterization of escherichia coli dnaB::Tn10 insertion mutations.
Exploitation of the ability of the ban protein encoded by phage P1 to compensate for dnaB-defective host mutations, allowed the isolation of dnaB::Tn10 insertion mutations. The presence of P1bac prophage was required for survival of dnaB::Tn10 mutants, and such lysogens were cryosensitive. The insertions were shown to map in dnaB by transduction and this was confirmed by complementation analysis. The dnaB::Tn10 (P1bac) strains were non-permissive for lambda growth but did support the growth of lambda-dnaB+ specialized transducing phage. No antigenically active dnaB product could be detected by immunologic assays using either of two methods. In addition, it was shown that the observe cryosensitivity of P1bac suppression was a direct result of reversible inactivation of the ban protein at low temperature.
D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003080:Cold Temperature; D003090:Coliphages / Q000235:genetics*; D004265:DNA Helicases; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D009154:Mutation; D011995:Recombination, Genetic; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D014764:Viral Proteins; D004265:DNA Helicases; C017048:ban protein, Enterobacteria phage P1