[Influence of copper in combination with other trace elements on the activity of trypsin (author's transl)].
The activity of trypsin was determined by using N alpha-Benzoyl-L-arginin-p-nitroanilid (L-BAPA) as a substrate, and adding either the elements Cu, Zn, Fe, Ni, and Co alone or in combination with copper in the concentration range of 0.9 x 10(-7) to 0.9 x 10(-4) moles Men+/l. Cu and Zn don't elevate the activity of trypsin but if they are added separately in very small concentrations. They lower the activity at higher concentrations. The other trace elements elevate the trypsin activity over the whole concentration range investigated. When copper is used in combination with the other trace elements there is no additional effect on trypsin activity. Here activities are somewhere in between the values when elements are added separately. A competing effect for the bounding sites of the trypsin is supposed. In this way it is possible to explain the non-linearity of the turnover rate curves of the experiments with the combined trace elements.
D001586:Benzoylarginine Nitroanilide / Q000378:metabolism; D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D009532:Nickel / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D014361:Trypsin Inhibitors; D003035:Cobalt; D003300:Copper; D009532:Nickel; D001586:Benzoylarginine Nitroanilide; D007501:Iron; D014357:Trypsin; D015032:Zinc
Beeinflussung der Trypsinaktivität durch Kupfer in Kombination mit anderen Spurenelementen.