title: Extracellular matrix synthesis in the chick embryo lateral plate prior to and during limb outgrowth.
abstract: Little is known at the present time about the molecular basis and mechanisms of morphogenesis. The present study is an attempt to determine what influence the extracellular matrix has on the initial outgrowth of the limb bud. Stage -12 to -18 chick embryo lateral plates were examined in relation to proline and sulfate incorporation into collagen and proteoglycan. The flank and limbs incorporated the same amount of labeled proline and sulfate before stage 16. At stage 16 the flank began to incorporate more of both isotopes until at stage 18 there was twice as much incorporation into the flank as into the limbs. The flank and limbs contained the same type of collagen during the period examined. The limbs contained both large and small proteoglycans but the flank contained only small proteoglycans. These data suggest that the extracellular matrix in the flank and limb regions may play a role in limb outgrowth and that the limb buds at these stages may be more inclined toward cartilage development.
mesh_terms: D000367:Age Factors; D000818:Animals; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis; D005110:Extracellular Space / Q000378:metabolism*; D005121:Extremities / Q000196:embryology* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism; D009024:Morphogenesis; D011392:Proline / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis; D013431:Sulfates / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D003094:Collagen; D011392:Proline; D003012:Microbial Collagenase