Effect of mitochondrial functions on synthesis of yeast cytochrome c.
The effects of the mitochondrial protein synthesis inhibitor chloramphenicol and the mitochondrial F0 adenosine triphosphatase inhibitor oligomycin on the synthesis of nucleus-encoded cytochrome c protein were studied. Both inhibitors stimulated cytochrome c protein synthesis in the derepressed state (growth in media containing 2% raffinose) but had no effect on the synthesis of the cytochrome c protein in the repressed state (growth in media containing 5% glucose). Oligomycin uncoupled the synthesis of the apoprotein from its processing into the hemoprotein. Neither antibiotic had a significant effect on the rate of glucose repression of cytochrome protein synthesis. The kinetics of cytochrome c derepression and the effects of these two antibiotics on these kinetics were also studied. Cells were derepressed by transfer from glucose- to faffinose-containing media, and the rate of cytochrome c synthesis increased from the repressed to the derepressed level during the second hour of derepression. Chloramphenicol delayed this derepression, but after 5 h the rate of cytochrome c protein synthesis increased to twice the rate of synthesis in uninhibited cells. On the other hand, oligomycin inhibited derepression of cytochrome c. These results are discussed with respect to the effects of mitochondrial function in the derepressed and repressed states and during the processes of repression and derepression of cytochrome c.
D002701:Chloramphenicol / Q000494:pharmacology; D003574:Cytochrome c Group / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000502:physiology*; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption; D011887:Raffinose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
D003574:Cytochrome c Group; D005656:Fungal Proteins; D009840:Oligomycins; D002701:Chloramphenicol; D005947:Glucose; D011887:Raffinose