title: The lambda phage att site: functional limits and interaction with Int protein.
abstract: Site specific integrative recombination of bacteriophage lambda involves unequal partners. The minimal phage att site is composed of approximately 240-base pairs and four distinct binding sites for Int protein, at least three of which are crucial for function. This 'donor site' recombines efficiently with a smaller 'recipient site' that lacks the extensive interactions with Int protein.
mesh_terms: D001287:Attachment Sites, Microbiological; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004265:DNA Helicases / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D019426:Integrases; D008242:Lysogeny; D010957:Plasmids; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004279:DNA, Viral; D014764:Viral Proteins; D019426:Integrases; D004265:DNA Helicases