title: Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts.
abstract: Lysates of barley chloroplasts release more radioactivity into acid soluble form from UV-irradiated and alkylated-depurinated E. coli [3H] DNA than from intact DNA. By means of affinity chromatography on depurinated DNA-cellulose and/or UV irradiated DNA-cellulose and by electrophoresis in polyacrylamide gels, four activities on depurinated DNA were separated. One of these contained activity against heavily UV-irradiated /270 J.m-2/ native DNA. In addition, two other nucleases specific towards UV-DNA were separated. One of them was active on native and heat denatured DNA irradiated with 10 J . m-2 UV, whereas the other was predominantly active on native UV-irradiated DNA.
mesh_terms: D002736:Chloroplasts / Q000378:metabolism*; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair; D002523:Edible Grain / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D001467:Hordeum / Q000378:metabolism*; D011687:Purines; D011740:Pyrimidine Dimers / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D011687:Purines; D011740:Pyrimidine Dimers; D004247:DNA; D004720:Endonucleases