Analytical subcellular fractionation of rabbit alveolar macrophages with particular reference to the subcellular localisation of pyridine nucleotide-dependent superoxide-generating systems and superoxide dismutase.
Normal and Bacillus Calmette-Guerin (BCG) vaccine-induced rabbit alveolar macrophage homogenates were fractionated by isopycnic density gradient centrifugation. Superoxide dismutase-inhibitable NAD(P)H-dependent nitro-blue tetrazolium reductase was found localised to endoplasmic reticulum and mitochondria. The normal macrophages tended to contain more of this activity than the BCG-induced macrophages. Two superoxide dismutases were found: cyanide-sensitive superoxide dismutase was predominantly present in the cytosol, with a small proportion in mitochondria; cyanide-resistant superoxide dismutase was found confined to mitochondria. Neither differed in specific activity betw-en the normal and BCG-induced macrophages.
D000818:Animals; D002458:Cell Fractionation; D004072:Digitonin; D011756:Diphosphates; D008264:Macrophages / Q000201:enzymology* / Q000648:ultrastructure; D009163:Mycobacterium bovis; D009246:NADH Tetrazolium Reductase / Q000032:analysis*; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D010100:Oxygen / Q000378:metabolism*; D011817:Rabbits; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000032:analysis*; D013481:Superoxides / Q000378:metabolism*
D011756:Diphosphates; D013481:Superoxides; D013482:Superoxide Dismutase; D009246:NADH Tetrazolium Reductase; D009247:NADH, NADPH Oxidoreductases; D004072:Digitonin; D010100:Oxygen