Colorimetric determination of serum 5'-nucleotidase without deproteinization.
We present a sensitive colorimetric determination of 5'-nucleotidase based on the measurement of liberated phosphate by reaction with stannous chloride/molybdenum blue. No protein precipitation is required. The method is simple, sensitive, economical, and easy to use, and involves relatively stable reagents.
D015720:5'-Nucleotidase; D000469:Alkaline Phosphatase / Q000097:blood; D003124:Colorimetry; D006801:Humans; D009532:Nickel / Q000494:pharmacology; D009708:Nucleotidases / Q000097:blood*; D010710:Phosphates / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D010710:Phosphates; D012967:Sodium Dodecyl Sulfate; D009532:Nickel; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase