Human schwann cells in tissue culture: histochemical and ultrastructural studies.
Schwann cells cultures were established from 40 human peripheral nerves that underwent biopsy according to an explant-reexplantation technique that markedly reduces non-Schwann cells and achieves cultures greatly enriched in Schwann cells suitable for a wide range of studies. Described are the growth characteristics and histochemical, ultrastructural, and ultrastructural-cytochemical patterns of normal human cultured Schwann cells from 12 patients with demonstrable peripheral nerve abnormality. These findings serve as a basis of comparison when seeking abnormalities of Schwann cells grown from peripheral nerves of patients with putative dysschwannian neuropathies.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D010523:Peripheral Nervous System Diseases / Q000378:metabolism / Q000473:pathology*; D012583:Schwann Cells / Q000378:metabolism / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure*