title: Reconstitution of the hepatic asialoglycoprotein receptor with phospholipid vesicles.
abstract: A solubilized detergent-free preparation of the hepatic binding protein specific for asialoglycoproteins associates spontaneously with small unilamellar lipid vesicles. This process is independent of the phase transition of the lipid and effectively restores the specific binding activity of the receptor protein. The insensitivity of the resulting lipid-protein complex to ionic strength provides evidence for a hydrophobic interaction. There is a perturbation of the lipid phase transition concomitant with addition of the protein. Circular dichroism studies indicate that the protein undergoes a conformational change on association with lipid. Binding of specific ligand produces further physical changes in the receptor as indicated by alterations in the tryptophan fluorescence quenching pattern.
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D055592:Biophysical Phenomena; D001703:Biophysics; D002499:Centrifugation, Density Gradient; D002942:Circular Dichroism; D005454:Fluorescence Polarization; D006023:Glycoproteins / Q000378:metabolism*; D008024:Ligands; D008081:Liposomes; D011485:Protein Binding; D011487:Protein Conformation; D011663:Pulmonary Surfactants; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D006023:Glycoproteins; D008024:Ligands; D008081:Liposomes; D011663:Pulmonary Surfactants; D011956:Receptors, Cell Surface