Demonstration by immunofluorescence that the same cells from chick embryo aortas synthesize elastin and collagen types I and III.
Cells were isolated from the aortas of 17-day old chick embryos and they were stained with fluorescent antibodies specific for Type I collagen, Type I procollagen, Type III collagen, elastin and prolyl hydroxylase. The results indicated that the same cells simultaneously synthesize Type I procollagen, Type III procollagen and elastin. The synthesis of procollagens, and the presence of prolyl hydroxylase, in the same cells which synthesize elastin may well explain why elastin contains hydroxy-proline.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D004549:Elastin / Q000096:biosynthesis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011347:Procollagen / Q000096:biosynthesis*; D011393:Procollagen-Proline Dioxygenase / Q000096:biosynthesis; D011863:Radioimmunoassay
D011347:Procollagen; D003094:Collagen; D004549:Elastin; D011393:Procollagen-Proline Dioxygenase