Heavy and light particles of adeno-associated virus.
KB cells coinfected with adenovirus and adeno-associated virus (AAV) yielded two kinds of infectious AAV particles that banded in CsCl at densities of 1.45 and 1.41 g/cm(2), respectively. The 1.45 band was found to be composed of a heterogeneous group of viral particles that could be subfractionated by velocity sedimentation. The main component from this band had a smaller S value (109) than the main component from the 1.41 band (111S), although both had the same DNA/protein ratio and the same density in metrizamide gradients. Continuous-label experiments showed that early after infection, both components (1.45 and 1.41) were generated in the same amounts, but this was followed by a relative increase in the proportion of the 1.41 component over the 1.45 particles. Pulse-chase analysis failed to demonstrate a precursor-product relationship between these two bands. The slower-sedimenting components from the 1.45 band were unstable in CsCl and were present in a greater proportion early after infection. These particles contained DNA that was enriched for the terminal sequences of the AAV genomes and was accessible to digestion with micrococcal nuclease.
D001483:Base Sequence; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis*; D000229:Dependovirus / Q000032:analysis* / Q000254:growth & development; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D014771:Virion / Q000032:analysis
D004279:DNA, Viral; D004262:DNA Restriction Enzymes