Synthesis of molecular species of glycerophospholipids from diglyceride-labeled brain microsomes.
Selectivity of CDP-choline:diacylglycerol choline phosphotransferase and CDP-ethanolamine:diacylglycerol ethanolamine phosphotransferase for molecular species of diglyceride has been studied in rat brain microsomes in vitro. Diglyceride-labeled microsomes were prepared by incubation with labeled sn-glycerol-3-phosphate; the microsomes were then incubated with CDP-choline or CDP-ethanolamine for different time intervals. Experimental data extrapolated to zero-time incubation were taken into account for evaluating species specificity. A small selectivity for diglyceride species has been demonstrated for the choline phosphotransferase, but the ethanolamine phosphotransferase was found to convert hexaenoic diglyceride into phospholipid at the highest rate.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003565:Cytidine Diphosphate / Q000378:metabolism; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D004982:Ethanolaminephosphotransferase / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats
D004075:Diglycerides; D004983:Ethanolamines; D005989:Glycerides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D003566:Cytidine Diphosphate Choline; D003565:Cytidine Diphosphate; D010770:Phosphotransferases; D004982:Ethanolaminephosphotransferase; D002798:Diacylglycerol Cholinephosphotransferase