Nucleotide sequence of a cloned fragment of rat mitochondrial DNA containing the replication origin.
The nucleotide sequence was determined for the 717 bp HapII subfragment (HapEcoA5) of the EcoRI-A fragment of rat mitochondrial DNA, which contains the heavy-strand replication origin. Analysis of the heavy-strand initiation segments released from the D-loop molecules has revealed that some 5'-ends of these initiation segments are linked to ribonucleotide(s) and are heterogeneous. Sequence analysis of the 5'-end portion of the initiation segment indicated that one of the start points of the deoxyribonucleotide polymerization corresponds to the 425th bp on the HapEcoA5. Two-fold rotational symmetry and palindrome structures, and a G-cluster sequence around the start point have been discussed in connection with unidirectional replication.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005796:Genes; D008099:Liver / Q000648:ultrastructure; D008928:Mitochondria / Q000378:metabolism; D051381:Rats
D004272:DNA, Mitochondrial; D004247:DNA; D004262:DNA Restriction Enzymes