New method for determining the extent of proline hydroxylation by measuring changes in the ratio of [4-3H]:[14C]proline in collagenase digests.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis; D002250:Carbon Radioisotopes / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism; D006168:Guinea Pigs; D006900:Hydroxylation; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism; D011392:Proline / Q000032:analysis / Q000378:metabolism*; D014316:Tritium / Q000032:analysis*
D002250:Carbon Radioisotopes; D014316:Tritium; D003094:Collagen; D011392:Proline; D003012:Microbial Collagenase