Cell-free translation of avian erythroblastosis virus RNA.
Avian erythroblastosis virus (AEV) RNA rescued from nonproducer cells by superinfection with a helper virus is translated into three polypeptides in the messenger-dependent rabbit reticulocyte lysate. A 75,000 molecular weight polypeptide (P75AEV) is synthesized from 28S RNA and is encoded by the 5' section of the AEV RNA, including gag-related and AEV-specific sequences. The P75AEV synthesized in infected cells and the P75AEV synthesized in the cell-free system are electrophoretically identical. A 44,000 molecular weight polypeptide (P44AEV) is synthesized from 20-24S RNA, apparently from the 3' section of the AEV-specific RNA sequence. A minor 37,000 molecular weight polypeptide (P37AEV) is synthesized from 20S AEV RNA. A comparison is drawn between the cell-free products of MC29 and AEV RNAs.
D001355:Alpharetrovirus / Q000235:genetics*; D001354:Avian Leukosis Virus / Q000235:genetics*; D002474:Cell-Free System; D005814:Genes, Viral; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
D012367:RNA, Viral; D014764:Viral Proteins