Chemical characterization of adrenocorticotropin and a novel peptide biosynthesized from a pituitary adenoma of a patient with Cushing's disease.
ACTH and another unidentified peptide have been biosynthesized in vitro and purified from a pituitary adenoma of a patient with Cushing's disease. The new peptide had an apparent molecular weightof 4000 daltons and was more acidic than ACTH as analyzed by sodium dodecyl sulfate/urea and acidic (pH 4.5) polyacrylamide gel electrophoreses, respectively. Sequence studies revealed the presence of [35S]methionine at position 3 of the new peptide. High performance liquid chromatographic analysis of [35S]methionine-labeled tryptic fragments of the peptide revealed that this peptide contained gamma MSH- (1-7) within its sequence. Our observations raise the possibility that this peptide contains at its N-terminus gamma MSH, a putative peptide proposed by Nakanishi et al.
D000236:Adenoma / Q000150:complications / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D003480:Cushing Syndrome / Q000150:complications / Q000378:metabolism*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D009074:Melanocyte-Stimulating Hormones / Q000096:biosynthesis*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010446:Peptide Fragments / Q000032:analysis; D010911:Pituitary Neoplasms / Q000150:complications / Q000378:metabolism*
D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D006854:Hydrocortisone