Liposomes as vehicles for cellular incorporation of biologically active macromolecules.
Lipid vesicles (liposomes) have recently been shown to be a useful vehicle for the delivery of a variety of compounds to cultured cells. Using large unilamellar vesicles composed of phosphatidylserine [LUV(PS)] we were able to encapsulate poliovirus and purified poliovirus ribonucleic acid (RNA) and show that it can be delivered efficiently to cells in an infectious form. LUV-entrapped poliovirus RNA produced infectious titers 100-fold higher than comparable RNA preparations delivered to cells by other techniques. We have made a quantitative analysis of the uptake and infectivity of the vesicle-encapsulated RNA by using various ratios of RNA copies per vesicle and by determining the percentage uptake of labelled lipid and RNA by HeLa cells.
D002478:Cells, Cultured / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000378:metabolism / Q000502:physiology*; D046911:Macromolecular Substances / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism; D017955:Poliovirus / Q000302:isolation & purification / Q000472:pathogenicity; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism*
D008081:Liposomes; D046911:Macromolecular Substances; D010718:Phosphatidylserines; D012367:RNA, Viral