Inducibility of spleen focus-forming virus by BrdUrd is controlled by the differentiated state of the cell.
All Friend cells--except thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)-deficient mutants--are highly inducible for the release of biologically active spleen focus-forming virus (SFFV) after exposure to BrdUrd. We studied SFFV production in somatic cell hybrids made between Friend leukemia cells (FLC) and cells expressing various differentiation programs. High inducibility of SFFV and release of constitutive Friend virus (FV) and SFFV are eliminated in all hybrids in which the potential for erythroid differentiation is suppressed. FV release and its induction by BrdUrd are unchanged in hybrids that maintain the expression of erythroid differentiation.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002999:Clone Cells; D005622:Friend murine leukemia virus / Q000378:metabolism*; D006822:Hybrid Cells / Q000502:physiology; D007940:Leukemia L5178; D007942:Leukemia, Experimental; D051379:Mice; D051381:Rats; D012190:Retroviridae / Q000378:metabolism*; D013045:Species Specificity; D014779:Virus Replication / Q000187:drug effects
D001973:Bromodeoxyuridine