Cyclic AMP increase by histamine and its analogues in guinea-pig submandibular gland.
The effect of histamine and its analogues on the cyclic AMP level in chopped guinea-pig submandibular glands was investigated. These agonists increased the cyclic AMP level dose-dependently. The ED50 values for histamine and 4-methylhistamine were approximately 1.3 x 10(-5) M and 2.2 x 10(-5) M, respectively. The efficacy of 4-methylhistamine was higher than that of histamine. 2-(2-Pyridyl)-ethylamine was far less effective compared with histamine or 4-methylhistamine. Metiamide markedly antagonized the cyclic AMP response to histamine and 4-methylhistamine, but mepyramine was ineffective. A combination of aminoguanidine and quinacrine had no influence on the effect of histamine on the cyclic AMP level. These results indicate the H2-type histamine receptors which mediate the cyclic AMP increase are present in the guinea-pig submandibular gland.
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008761:Methylhistamines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects; D011970:Receptors, Histamine H2 / Q000187:drug effects; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*
D008761:Methylhistamines; D011725:Pyridines; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; C025566:4-methylhistamine; D006632:Histamine; C006183:2-(2-aminoethyl)pyridine; D000242:Cyclic AMP