[Modification of the agarose drop micromethod for studying mouse macrophage migration inhibition factor].
A modification of the drop agarose micromethod for the study of the factor inhibiting the migration of mouse macrophages is proposed. The syngeneic system was used instead of type RPMI-1640 complex culture media and calf embryonal serum, commonly used in the work with mouse macrophages. The production of the factor inhibiting the migration of macrophages by sensitized lymphocytes from mice of different strains has been shown to occur in vitro under the influence of pertussis corpuscular antigen. The method of obtaining supernatant containing macrophage migration inhibiting factor, from immune mouse lymphocyte culture is proposed. This supernatant may be used for, the analysis of the above-mentioned factor and other lymphokins.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D006824:Hybridization, Genetic; D008214:Lymphocytes / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis*; D008722:Methods; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012685:Sepharose; D013045:Species Specificity
D000942:Antigens, Bacterial; D008263:Macrophage Migration-Inhibitory Factors; D012685:Sepharose
Modifikatsiia kapel'nogo agaroznogo mikrometoda izucheniia faktora tormozheniia migratsii myshinykh makrofagov.