Selection of antibodies for the immunofluorescent localisation of cyclic GMP in the central nervous system.
Radioimmunoassay techniques have been used to determine why only a small number of antibodies against cyclic GMP are able to show immunofluorescent staining in the central nervous system. However, neither the titre, avidity nor specificity as determined in liquid-phase radioimmunoassay employing [3H]cyclic GMP, have been able to predict whether a given antibody will show specific staining, or account for the different sites of immunofluorescent localisation observed with these antibodies. These observations are discussed in terms of the populations of antibody molecules employed in the radioimmunoassay and immunofluorescent procedures.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002490:Central Nervous System / Q000032:analysis*; D006152:Cyclic GMP / Q000032:analysis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry / Q000379:methods*; D066298:In Vitro Techniques; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
D000906:Antibodies; D006152:Cyclic GMP