Immunocytochemical localization of (Na+, K+)-ATPase in the goldfish optic nerve.
Antiserum to the catalytic subunit of goldfish brain (Na+, K+)-ATPase has been employed at the electron microscopic level by means of the peroxidase-antiperoxidase immunohistochemical method. In optic nerve, antigenic sites are restricted to the nodes of Ranvier. No reaction product is detected in underlying internodal neurolemma. Outgrowing neurites for cultured retinal explants devoid of glial ensheathment exhibit a continuous distribution of the enzyme subunit. Antibodies against eel electroplax (Na+, K+)-ATPase cross-react with the goldfish brain enzyme and show a similar immunocytochemical distribution pattern.
D000818:Animals; D003429:Cross Reactions; D046508:Culture Techniques; D006054:Goldfish; D006651:Histocytochemistry; D007074:Immunoglobulin G / Q000276:immunology; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D009900:Optic Nerve / Q000201:enzymology* / Q000648:ultrastructure; D012160:Retina / Q000201:enzymology / Q000276:immunology; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000276:immunology
D007074:Immunoglobulin G; D000254:Sodium-Potassium-Exchanging ATPase