title: Interaction of selectively spin-labeled 70S ribosomes with tRNAPhe. An electron paramagnetic resonance study.
abstract: 70S ribosomes from Escherichia coli, selectively spin labeled on the SH groups of proteins S18, S12, S21, S17, and L27, were used to study the formation of the tertiary complex ribosome-poly(U)-tRNAPhe. Most of these ribosomal proteins are located in the region of binding of tRNA. The electron paramagnetic resonance observable structural change suggests a loosening of the ribosome structure upon binding of the tRNA molecule.
mesh_terms: D001205:Ascorbic Acid; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008968:Molecular Conformation; D011072:Poly U / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013113:Spin Labels
chemical_list: D012346:RNA, Transfer, Amino Acyl; D012269:Ribosomal Proteins; D013113:Spin Labels; D011072:Poly U; D001205:Ascorbic Acid