The state of actin in activated human platelets.
The state of platelet actin was determined using a fluorescent method of DNAse I inhibition. Activation of human platelets resulted in mobilization of DNAse-available actin. When platelets were activated with ADP the change in the state of actin was gradual and preceded the secretion. When thrombin was used as an activator, a sharp and rapid decrease in the DNAse-available actin was observed which paralleled the secretion. Inhibition of ADP-induced aggregation (and secretion) by EDTA resulted in a decrease in the rate of change on actin. Inhibition of the thrombin-induced aggregation (but not secretion) by EDTA did not affect the change in the state of actin.
D000199:Actins / Q000097:blood*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004720:Endonucleases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010974:Platelet Aggregation / Q000187:drug effects; D013917:Thrombin / Q000378:metabolism
D000199:Actins; D000244:Adenosine Diphosphate; D004492:Edetic Acid; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D013917:Thrombin