Interaction of cytomegalovirus immune complexes with host cells.
After adsorption to host cells of a mixture of cytomegalovirus (CMV) and immunoglobulin G (IgG)-anti CMV, at least 99% of the surviving fraction consisted of infectious virus-antibody complexes which could be neutralized by anti-human IgG antibody. The virus-antibody complexes penetrated into cells and were uncoated more slowly than native virus. When the virus-antibody complexes were treated with anti-human IgG antibody before adsorption was allowed to take place, anti-human IgG antibody immune complexes were adsorbed to host cells less readily and uncoated less efficiently than were untreated complexes. Furthermore, after adsorption, anti-human IgG antibody-IgG-CMV complexes were further retarded in their penetration and uncoating. These observations suggest that neutralization of CMV by IgG-anti-CMV resulted from interference with the normal mechanisms of penetration and uncoating. Anti-human IgG antibody enhanced the degree of neutralization by augmenting the inhibitory effect of antibody on these stages of virus-host interaction and also reduced adsorption of the complex.
D000327:Adsorption; D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000276:immunology*; D000936:Antigen-Antibody Complex; D002478:Cells, Cultured / Q000382:microbiology*; D003587:Cytomegalovirus / Q000276:immunology* / Q000502:physiology; D005347:Fibroblasts; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D009500:Neutralization Tests; D010948:Viral Plaque Assay
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G