[Non-phosphorylative transglycosylation in extracts from white and red muscles of carp (Cyprinus carpio L.)].
The enzymatic, nonphosphorylative reaction of transglycosylation in extracts from white and red muscles of the carp was studied. Amylomaltase (EC 2.4.1.25) activity was assayed using p-nitrophenyl-N-maltoside as substrate and the amount of p-nitrophenyl-N-maltotrioside was assumed as a measure of the enzyme activity. The pH and temperature optimum of the reaction was examined. In both types of muscles significant differences in transglycosylative activity and its decrease immediately after prolonged transport of carps were found.
D000818:Animals; D050260:Carbohydrate Metabolism; D002347:Carps / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D006004:Glycogen Debranching Enzyme System; D006031:Glycosylation; D066298:In Vitro Techniques; D009132:Muscles / Q000378:metabolism*; D014020:Tissue Extracts / Q000378:metabolism
D006004:Glycogen Debranching Enzyme System; D014020:Tissue Extracts; D005964:Glucosyltransferases; C020569:4 alpha-glucanotransferase
Niefosforylacyjna transglikozylacja w wyciagach z mieśni białych i czerwonych karpia (Cyprinus carpio l.).