title: Cyclic AMP phosphodiesterases of the rat ovary. Oestrous cycle dependent activity change of high affinity form.
abstract: In the rat ovary, from prenatal to adult stages, two electrophoretically distinct forms of cyclic AMP phosphodiesterase (E.C.: 3.1.4.17) occur. These forms were analysed for their affinity towards cAMP in both crude and partially purified preparations. Whereas the slow electrophoretic form, which has a low affinity towards cAMP, shows a linear Lineweaver-Burk plot, the fast electromorph exhibits anomalous kinetic behaviour in that cAMP affinity appears to increase with decreasing cAMP-concentration. This form shows dramatic alteration of maximum velocity during the oestrous cycle, attaining maximum activity at metoestrus. This rise of activity is sensitive to inhibition of protein synthesis.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004592:Electrophoresis, Starch Gel; D004971:Estrus; D005260:Female; D007700:Kinetics; D010053:Ovary / Q000196:embryology / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats
chemical_list: D003609:Dactinomycin; D003513:Cycloheximide; D015105:3',5'-Cyclic-AMP Phosphodiesterases