[Effects of basic proteins of low molecular weight on the phosphohydrolase and phosphotransferase activities of microsomal glucose-6-phosphatase in adult monkey hepatocytes (author's transl)].
The effects of histone 2A and some polycations on microsomal carbamylphosphate:D-glucose phosphotransferase and glucose-6-phosphate phosphohydrolase activities (D-glucose-6-phosphate phosphohydrolase, EC 3.1.3.9), have been investigated. 1. Histone 2A and polycations activate the two enzymic activities. At a constant cation concentration, this activation increases with the number of cationic groups per molecule. 2. Activation by histone 2A is related to its fixation on microsomal membranes. This fixation varies with quantities of histones and pH. 3. The nature of the interactions between histones and microsomal membranes is shown to be electrostatic, probably between the cationic groups of histones and the anionic group of membranous lipids. 4. Kinetic analysis reveal that histone 2A increases the maximal reaction velocity but does not affect the apparent Michaelis constant values for the substrates. 5. The role played by the cationic groups of histone 2A on the microsomal glucose 6-phosphatase, is discussed.
D000818:Animals; D002412:Cations; D004789:Enzyme Activation / Q000187:drug effects; D004901:Erythrocebus patas; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006657:Histones / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*
D002412:Cations; D006657:Histones; D010770:Phosphotransferases; C026823:carbamylphosphate-D-glucose phosphotransferase; D005952:Glucose-6-Phosphatase
Effet de petites protéines basiques sur les activitiés phosphohydrolase et phosphotransférase de la glucose-6-phosphatase microsomique des hépatocytes de singe.