title: Multienzyme complex for metabolic channeling in mammalian DNA replication.
abstract: In the DNA-synthesizing phase (S phase) of CHEF/18 Chinese hamster embryo fibroblast cells, six enzymes associated with DNA metabolism, including DNA polymerase (deoxynucleoside triphosphate:DNA deoxynucleotidyl-transferase, EC 2.7.7.7), were largely localized in the nuclear region (karyoplasts). By contrast, in quiescent and G1 phase cells these enzymatic activites were mainly absent from the nucleus and were recovered in the cytoplasmic portion (cytoplasts). These nuclear (but not cytoplasmic) enzymatic activities cosedimented rapidly on sucrose density gradients. Further, the rapidly sedimenting enzyme activities were unique to cells in S phase. An organized supramolecular structure that allows channeling of metabolites into DNA was demonstrated by kinetics of nucleotide incorporation. "Permeabilized" cells selectively channeled incorporation of ribonucleoside diphosphates into DNA in preference to deoxyribonucleoside triphosphates. Deoxyribonucleoside triphosphate incorporation occurred when ribonucleoside-diphosphate reductase (2'-deoxyribonucleoside-diphosphate: oxidized-thioredoxin 2'-oxidoreductase, EC 1.17.4.1) activity was abolished by hydroxyurea. Our interpretation is that during DNA replication, the nucleus contains a complex of DNA precursor-synthesizing enzymes juxtaposed with the "replication apparatus" comprising DNA polymerase, other enzymes, and structural proteins. Functional integrity of this structure is impaired when one of its essential components is inactivated. We propose the name "replitase" for this multienzyme complex for DNA replication and suggest that it incorporates precursors rapidly and efficiently. Possibly its assembly signals the initiation of the S phase of the cell cycle.
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D002453:Cell Cycle; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003568:Cytidine Monophosphate / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003853:Deoxyribonucleosides / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism; D012263:Ribonucleosides / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D013937:Thymidine Kinase / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism
chemical_list: D003853:Deoxyribonucleosides; D009097:Multienzyme Complexes; D012263:Ribonucleosides; D012262:Ribonucleoside Diphosphate Reductase; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; C019979:cytidylate kinase; D009703:Nucleoside-Phosphate Kinase; D009701:Nucleoside-Diphosphate Kinase; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; D003568:Cytidine Monophosphate