Identification of a purine (P2) receptor linked to ion transport in a cultured renal (MDCK) epithelium.
1 Exogenous adenosine triphosphate (ATP) stimulates the short circuit current (SCC) in a cultured renal-derived epithelium (MDCK). Half-maximal stimulation is achieved at 1.91 X 10(-5) M ATP. 2 It is suggested that ATP interacts with a P2 purine receptor upon the basis of (a) agonist potency (ATP greater than adenosine diphosphate much greater than adenosine monophosphate, adenosine; ATP greater than uridine triphosphate greater than inosine triphosphate much greater than cytosine triphosphate, guanosine triphosphate); (b) the inhibition of the ATP response by quinidine (1 X 10(-3) M) but not by theophylline (1 X 10(-3) M). 3 Indomethacin (1 X 10(-5) M) inhibits the response of the cultured epithelium to ATP. 4 Prostaglandin E1 (PGE1) stimulates SCC but potentiates the effect of ATP on SCC. The divalent cationic ionophore A23187 (1 X 10(-6) M) transiently stimulates SCC itself and abolishes ATP-induced stimulation of the SCC.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002412:Cations / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004347:Drug Interactions; D004573:Electrolytes / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D011983:Receptors, Purinergic
D002412:Cations; D004573:Electrolytes; D011955:Receptors, Drug; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D000242:Cyclic AMP