The replacement of serum by SiO2 (aerosil) and 2-mercaptoethanol or diethanoldisulfide in the immunization and stimulation of spleen cell cultures.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002478:Cells, Cultured; D004220:Disulfides / Q000494:pharmacology*; D005260:Female; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D012822:Silicon Dioxide / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
D004220:Disulfides; D008623:Mercaptoethanol; D012822:Silicon Dioxide