[Effect of ethylenediamine derivatives on Na+, K+-ATPase and K+-p-nitrophenyl phosphatase activity of brain enzyme preparations].
The activity of Na+, K+-ATPase preparations was studied as affected by ethonium and its analogs. Ethonium is found to be able of producing a strong inhibitory effect on the Na+, K+-ATPase and K+-n-nitrophenyl phospnatase activities (I50 = 2.5.10(-5) and 6.5.10(-5) M, respectively). The inhibition is irreversible under experimental conditions and is reduced when the concentration of protein and sodium ions in the incubation medium rises. A dependence is found between the inhibitory effect of the analogs and the length of their hydrocarbon radical (the inhibition maximum corresponds to bisquarternary ammonium compound with the hydrocarbon radical consisting of 10 links) and some kinetic characteristics of this effect are studied. It is supposed that the mechanism of the inhibitory effect is due to the ethonim binding by the hydrocarbon radical in the preparation hydrophobic zone, the electrostatic interaction being not excluded.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D005029:Ethylenediamines / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013329:Structure-Activity Relationship
D005029:Ethylenediamines; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase
O vliianii riada proizvodnykh etilendiamina na Na+, K+-ATP-aznuiu i K+-n-nitrofenilfosfataznuiu aktivnosti fermentnykh preparatov mozga.