title: Assembly of the mitochondrial membrane system. Sequence of the oxi 2 gene of yeast mitochondrial DNA.
abstract: The region of mitochondrial DNA (mtDNA) containing the oxi 2 locus has been sequenced in a rho- clone (DS40) derived from the respiratory competent strain D273-10B/A48 of Saccharomyces cerevisiae. The DS40 clone was established to have retained only genetic markers in the oxi 2 locus and to have a segment of mtDNA extending from 18.6 to 24.3 units of the wild type map. The mitochondrial genome of DS40 includes a sequence that has been tentatively identified as the structural gene of Subunit 3 of cytochrome oxidase. The coding sequence is 810 nucleotides long and generates a protein with a molecular weight of 30,340. The amino acid composition of the oxi 2 gene product deduced from the nucleotide sequence is in agreement with the composition of the purified Subunit 3 of yeast cytochrome oxidase. The orientation of the DS40 mtDNA segment relative to wild type mtDNA indicates that the oxi 2 gene is transcribed from the same DNA strand as the oxi 1 and several other mitochondrial genes.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis / Q000235:genetics; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D003576:Electron Transport Complex IV / Q000032:analysis / Q000235:genetics*; D005796:Genes; D008928:Mitochondria / Q000032:analysis*; D009154:Mutation; D009711:Nucleotides / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000201:enzymology / Q000235:genetics
chemical_list: D000596:Amino Acids; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D009711:Nucleotides; D003576:Electron Transport Complex IV; D004262:DNA Restriction Enzymes; D000251:Adenosine Triphosphatases