Hairy cell leukemia: establishment of a cell line and its characteristics.
A hairy cell leukemia (HCL) line, ZK-H, was established from peripheral blood of a 69-year-old male patient. The ZK-H cells and the patient's original hairy cells shared the same surface properties; both possessed membrane-bound IgG with kappa light chains and villous surface structures. The ZK-H line carried Epstein-Barr virus (EBV)-determined nuclear antigen, but the patient's fresh leukemic cells lacked this antigen. Morphologically, the ZK-H cells appeared lymphoblastoid and more primitive than the preculture cells. The ZK-H line had a hyperdiploid chromosome constitution of 47 and trisomy no. 2. The presence of membrane-bound immunoglobulin and of B-cell tropic EBV in this cell line provides further evidence for the B-cell nature of HCL in this patient
D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007621:Karyotyping; D007943:Leukemia, Hairy Cell / Q000276:immunology / Q000473:pathology*; D008297:Male; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D000956:Antigens, Viral; D011947:Receptors, Antigen, B-Cell