title: Effect of fractions of Ethiopian And Norwegian colostrum on rotavirus and Escherichia coli heat-labile enterotoxin.
abstract: Samples of colostrum from both Ethiopian and Norwegian women contained antirotavirus activities of immunoglobulin and non-immunoglobulin nature. No significant differences in rotavirus immunoglobulin A or in rotavirus-inhibiting activity were found between samples from the two countries. The non-immunoglobulin inhibitory activity was trypsin sensitive and heat stable (100 degrees C for 10 min). Escherichia coli heat-labile enterotoxin antibodies were measured in the colostrum samples by enzyme-linked immunosorbent assay. No E. coli enterotoxin-specific immunoglobulin A was detected, possibly due to the high background caused by the nonspecific adsorption of immunoglobulin A to the enzyme-linked immunosorbent assay plates in the absence of toxin. A total of 5 of 15 Ethiopian colostrum samples and 0 of 11 Norwegian colostrum samples neutralized the effect of E. coli heat-labile enterotoxin on YI adrenal cells. Both the Ethiopian and the Norwegian colostrum samples contained a non-immunoglobulin enterotoxin-inhibitory activity when the toxin was measured by enzyme-linked immunosorbent assay. This inhibitory activity was not trypsin sensitive, and extraction by chloroform-methanol indicated that the inhibitor was of a lipid nature.
mesh_terms: D000992:Antitoxins / Q000032:analysis; D000998:Antiviral Agents / Q000032:analysis*; D001427:Bacterial Toxins; D003126:Colostrum / Q000032:analysis* / Q000276:immunology; D004768:Enterotoxins / Q000037:antagonists & inhibitors* / Q000276:immunology; D029968:Escherichia coli Proteins; D005002:Ethiopia; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D009664:Norway; D012087:Reoviridae / Q000254:growth & development*; D012401:Rotavirus / Q000254:growth & development* / Q000276:immunology
chemical_list: D000992:Antitoxins; D000998:Antiviral Agents; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; C027538:heat-labile enterotoxin, E coli