Effect of N-terminal portion of pro-opiomelanocortin on aldosterone release by human adrenal adenoma in vitro.
An adrenal cortex adenoma, surgically removed from a female patient with primary aldosteronism, was used to examine the effect of ACTH, angiotensin II, gamma 3-MSH, and the N-terminal fragment of pro-opiomelanocortin purified from porcine anterior pituitaries on aldosterone release in vitro. Primary cultures of tumor cells were incubated as a monolayer in a 96-well microtitration plate and the aldosterone release was measured in the incubation medium after 2 h of incubation in the presence of absence of different concentrations of the peptides. On a molar basis, the N-terminal portion of pro-opiomelanocortin seems to have the highest activity of all of the peptides assayed.
D000236:Adenoma / Q000378:metabolism*; D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D008875:Middle Aged; D010446:Peptide Fragments / Q000494:pharmacology*; D010908:Pituitary Hormones, Anterior / Q000494:pharmacology*; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors / Q000494:pharmacology*; D013552:Swine
D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D000804:Angiotensin II; D000450:Aldosterone; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones