The uptake and release of benzo[a]pyrene and benzo[e]pyrene in vitro by Syrian hamster embryo cells as a function of serum concentration.
A quantitative study on the in vitro uptake of benzo[a]pyrene (B[a]P) and benzo[e]pyrene (B[e]P) by Syrian hamster embryo cells and the induction of sister chromatid exchange (SCE) has been carried out. The amounts of B[a]P and B[e]P taken up by the cells decreases as does the induction of SCEs by B[a]P when the concentration of serum in the culture medium increases. It appears that serum prevents (B[a]P or B[e]P uptake. We have observed no significant differences between the two hydrocarbons regarding uptake by cells; chromatographic results show however that B[a]P is metabolized by these cells, while B[e]P is not. Our results suggest that serum inhibits B[a]P and B[e]P uptake and hence decreases the number of SCEs.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000097:blood / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae; D004622:Embryo, Mammalian; D005260:Female; D066298:In Vitro Techniques; D008647:Mesocricetus; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013997:Time Factors
D001580:Benzopyrenes; D001564:Benzo(a)pyrene; C026487:benzo(e)pyrene