The potential of recombination for the production of foot-and-mouth disease virus vaccine strains.
Biological proof of recombination in foot-and-mouth disease virus has been provided by studies on a large suite of conditional lethal mutants and a recombination map of approximately 70 mutations has been produced. However, recombination is a highly variable phenomenon and this has cast doubt on its validity and certainly on its applicability to such practical purposes as the production of good vaccine strains. Evidence is presented to demonstrate (1) that the genetic map has a good correlation with the biochemical map of polypeptide coding sequence for the genome and (2) that recombinants can be isolated bearing biochemical and biological markers from both parents. Finally, the analysis of 36 recombinants from a cross involving a cattle-attenuated and a cattle-virulent virus is described. These results have enabled us to identify the location of biological properties such as a mouse virulence and good growth in BHK cells on the genome of these two parent viruses.
D000818:Animals; D005537:Aphthovirus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D005814:Genes, Viral; D051379:Mice; D009154:Mutation; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines
D014764:Viral Proteins; D014765:Viral Vaccines