Conversion of mammalian cyclic GMP-dependent protein kinase into modulator-dependent protein kinase (type II) in vitro.
The spontaneous conversion of mammalian cyclic GMP-dependent protein kinase (G-PK) into modulator-dependent protein kinase (type II) (M-PKII) in the absence of cGMP or histone was observed in vitro. The findings, together with similarity in substrate protein specificity, suggest that M-PKII is the catalytic subunit of mammalian G-PK.
D000818:Animals; D002850:Chromatography, Gel; D006152:Cyclic GMP / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D011494:Protein Kinases / Q000378:metabolism*
D006657:Histones; D011494:Protein Kinases; D006152:Cyclic GMP