title: Hormonal regulation of glycogen synthase: insulin decreases protein kinase sensitivity to cyclic AMP.
abstract: Rat hemidiaphragms incubated with epinephrine exhibited increases in cyclic AMP content and protein kinase activity which were proportional to the logarithm of the hormone concentration from 0.1--2 microM. The fraction of glycogen synthase made independent of glucose-6-P for activity (%I) decreased concomitantly, but correlated only with epinephrine concentrations up to 0.2 microM. Insulin (0--100 mU/ml) increased glycogen synthase %I in a dose-dependent manner with no change in cyclic AMP concentration. Protein kinase activity increased slightly at the lowest insulin concentration, then decreased slightly as glycogen synthase %I increased. Insulin was without effect when administered with a supramaximal dose of epinephrine. In the presence of submaximal epinephrine, insulin produced a dose-dependent increase in glycogen synthase %I which correlated with a decrease in protein kinase activity, without changing cyclic AMP. Insulin had no effect on the increases in cyclic AMP produced by varying levels of epinephrine. However, the activation of protein kinase activity by endogenous cyclic AMP was inhibited in the presence of insulin. The glycogen synthase %I response to epinephrine also was less sensitive in the presence of insulin. Insulin antagonizes the activation of cyclic AMP-dependent protein kinase by epinephrine without altering cyclic AMP levels.
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D006006:Glycogen Synthase / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
chemical_list: D007328:Insulin; D000242:Cyclic AMP; D006006:Glycogen Synthase; D011494:Protein Kinases; D004837:Epinephrine