title: The SV40 early region TATA box is required for accurate in vitro initiation of transcription.
abstract: The SV40 early region TATA box functions in vitro to fix initiation of transcription within a narrow region identical to that in vivo. In the absence of the TATA box region initiation occurs at multiple specific sites, as in vivo. However, certain sequences far upstream, crucial for efficient in vivo expression, are not essential in vitro.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002474:Cell-Free System; D004279:DNA, Viral / Q000235:genetics; D009876:Operon; D012319:RNA Polymerase II / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012319:RNA Polymerase II