The production of 5-HETE and leukotriene B in rat neutrophils from carrageenan pleural exudates.
Rat neutrophils isolated from three-hour carrageenan pleural exudates actively metabolize arachidonic acid into three major metabolites, HHT, 11-HETE and 15-HETE. However, in the presence of the calcium ionophore, A23187, or the non-ionic detergent, BRIJ 56, these cells also produce 5-HETE and LTB. The production of these lipoxygenase products is calcium dependent. While non-steroidal anti-inflammatory drugs do not affect 5-HETE or LTB production, BW 755C and ETYA inhibit formation of these metabolites from exogenously added arachidonic acid.
D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis* / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002351:Carrageenan / Q000494:pharmacology; D002592:Cetomacrogol / Q000494:pharmacology; D005122:Exudates and Transudates / Q000166:cytology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D006880:Hydroxy Acids / Q000096:biosynthesis; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D008084:Lipoxygenase / Q000097:blood; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010994:Pleura / Q000187:drug effects; D051381:Rats
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D006880:Hydroxy Acids; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D000001:Calcimycin; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D002351:Carrageenan; D002592:Cetomacrogol; D008084:Lipoxygenase