Plaque formation and purification of BK virus in cultured human urinary cells.
Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.
D001739:BK Virus / Q000032:analysis / Q000254:growth & development* / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D011120:Polyomavirus / Q000254:growth & development*; D014556:Urine / Q000166:cytology* / Q000382:microbiology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D004279:DNA, Viral