Identification by nucleotide sequence analysis of a goat pseudoglobin gene.
We have recently prepared a recombinant library of goat genomic DNA and have isolated clones containing th known goat globin genes. These include the alpha, gamma, beta C and beta A genes. In addition to these, another beta-like sequence has been observed. In this communication we report the complete nucleotide sequence of this gene, excluding a portion of the large intervening sequence. Several features suggest that this is a non-functional or pseudoglobin gene. The alterations include a frameshift mutation, substitution of the heme-binding histidines, a mutated termination codon, a change in the GT/AG excision sequence of the 5' end of the first intervening sequence, an AT rich sequence in the 3' untranslated region, and a mutated Hogness-Goldberg box. We conclude that this gene cannot function in the synthesis of globin.
D000818:Animals; D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D005914:Globins / Q000096:biosynthesis*; D006041:Goats; D006418:Heme; D006639:Histidine; D009154:Mutation; D012712:Serum Globulins
D003062:Codon; D004274:DNA, Recombinant; D012712:Serum Globulins; D006418:Heme; D006639:Histidine; D005914:Globins; D004262:DNA Restriction Enzymes