ESR spin label studies of the nucleosome core particle and histone core.
An imidazole spin label has been used to study the accessibility and conformational state of tyrosines in both the nucleosome core particles and histone core extracted from chicken erythrocytes. About 40% of the tyrosyl residues in the histone core can be labeled under nondenaturing conditions. However, less than 15% of the tryosyls in the nucleosome core particle can be labeled even at 200- to 300-fold M excess of label. The effect of urea on the conformational state of the spin-labeled tyrosyls in both the nuclesome core particles and the histone core has been studied. Ionic effects on the spin-labeled nucleosome core have been investigated. Several conformational transitions are observed in the range of 1 mM NaCl to 2.5 M NaCl. Three major transitions are found at 0.1 M to 0.6 M, 0.7 M to 1.8 M and 2 M to 2.5 M NaCl, respectively. The observed changes can be interpreted as swelling and conformational change of the inner histone core, gradual separation of DNA from the histone core, and tightening of the histone core.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000032:analysis*; D006657:Histones; D009690:Nucleic Acid Conformation; D009707:Nucleosomes; D011487:Protein Conformation; D012965:Sodium Chloride; D014443:Tyrosine; D014508:Urea / Q000494:pharmacology*
D006657:Histones; D009707:Nucleosomes; D014443:Tyrosine; D012965:Sodium Chloride; D014508:Urea