Chemosensitivity tests by a miniclone technique.
A modified miniclone method has been adopted to tissue culture testing of sensitivity to cytostatic drugs. The technique permits detailed microscopic clonal analysis of multiplication of single cells under conditions of 100% plating efficiency of certain selected established tumor lines or normal cells. Three different response parameters were distinguished--cytolysis, cytostasis and replicative capacity of cell fraction capable of multiplication. Notable differences were found among the five drugs tested--adriamycin, alkeran, BCNU, platinol and streptozocin.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002330:Carmustine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D008558:Melphalan / Q000494:pharmacology; D008722:Methods; D013311:Streptozocin / Q000494:pharmacology; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents; D013311:Streptozocin; D004317:Doxorubicin; D002945:Cisplatin; D008558:Melphalan; D002330:Carmustine