Inhibitor studies of phage T4 wild-type and mutant DNA polymerases. V. A. summary of kinetic and inhibitor data.
The DNA polymerases of phage T4 wild-type, the mutator mutant L98 and the antimutator mutant CB121 were purified about 100-fold free of foreign enzyme activities interfering with the polymerase assay. The enzymes were characterized as to thermostability, exonuclease activity and kinetic data with DNA template primer, deoxythymidine 5' -triphosphate, and a mixture of all four deoxynucleoside 5' -triphosphates. The effects of eight inhibitors of DNA synthesis on the three enzymes were determined (Schroeder and Jantschak 1978 and 1980, Jantschak and Schroeder 1980) and are compared here. The most selective inhibitor, pyridoxal 5' -phosphate, interacts with the active site of the polymerases while the two least discriminating drugs, distamycin A and actinomycin D, do not directly interact with the polymerases at all. The study was intended to test whether specific enzyme inhibitors elicit a differential response in temperature-sensitive structural variants of this enzyme and whether, in principle, structural variants of virus enzymes or the respective ts- mutants in vivo are suitable as a screening system for selective antiviral agents.
D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D043211:Exodeoxyribonuclease V; D005092:Exonucleases / Q000378:metabolism; D009154:Mutation; D019384:Nucleic Acid Synthesis Inhibitors; D013604:T-Phages / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D013942:Thymine Nucleotides / Q000378:metabolism
D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D005092:Exonucleases; D043211:Exodeoxyribonuclease V; C024157:thymidine 5'-triphosphate