The effect of benzyl beta-D-xyloside on keratan sulphate synthesis in ox articular cartilage.
Cartilage from adult bovine hock joints was incubated with [3H]galactose or [35S]sulphate in the presence of benzyl beta-D-xyloside. Radioisotope incorporation into proteoglycan was inhibited by the xyloside; the magnitude of this inhibition depended on the concentration of xyloside used. With 0.2mM xyloside radioisotope incorporation into keratan sulphate was not altered but inhibition was observed at xyloside concentrations of 1.0mM or higher. The decrease in radioisotope incorporation into keratan sulphate in the presence of 1.0mM benzyl beta-xyloside was directly related to a reduction in the average length of the keratan sulphate chains. This effect of beta-xyloside on keratan sulphate biosynthesis was markedly different from its effect on chondroitin sulphate biosynthesis.
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D005690:Galactose / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D006027:Glycosides / Q000494:pharmacology*; D007632:Keratan Sulfate / Q000096:biosynthesis* / Q000302:isolation & purification; D011509:Proteoglycans / Q000302:isolation & purification; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium
D006025:Glycosaminoglycans; D006027:Glycosides; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; C010807:xylosides; D014316:Tritium; D007632:Keratan Sulfate; D005690:Galactose