title: An erythropoietic stimulating factor similar to erythropoietin released by macrophages after treatment with silica.
abstract: An erythropoietic stimulating factor (ESF) can be detected in the supernatant from fetal liver and adult bone marrow and spleen cells when preincubated with the macrophage-specific cytotoxic agent, silica. Stimulation is observed in 12-day fetal liver CFU-E cultures in the absence of added erythropoietin (Ep). The concentration of ESF in the supernatant added to CFU-E cultures is dependent on the preincubated cell dose and the volume added. The stimulating activity is abolished when mice are hypertransfused and increased above normal values when mice are bled. A concentrated silica-treated spleen supernatant was able to stimulate erythropoiesis in the polycythemic mouse bioassay. It is concluded that the ESF is similar, if not identical, to Ep.
mesh_terms: D000740:Anemia / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D004921:Erythropoietin / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D011086:Polycythemia / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology*; D013154:Spleen / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D004921:Erythropoietin; D012822:Silicon Dioxide