[Sedimentation and electron microscopic characteristics of RNA isolated from a highly concentrated suspension of Sendai virus].
Sedimentation analysis in a lineal (5%--30%) sucrose concentration gradient of an RNA preparation isolated from a highly concentrated Sendai virus suspension revealed an additional peak with sedimentation coefficient 69S apart from previously known peaks of 46S (genome RNA), 30S and 18S (incomplete virus RNA). Electron microscopy methods showed the single-stranded molecules of 46S RNA to have a marked hairpin structure. Molecules of double-stranded 69S RNA have a specific structure: lineal parts alternate with areas in the form of rosettes. It is suggested that molecules of of 69S RNA are a product of incomplete hybridization between "plus" and "minus" complementary chains of virion RNA.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D008854:Microscopy, Electron; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D012367:RNA, Viral / Q000302:isolation & purification*
D012367:RNA, Viral
Sedimentatsionnaia i élektro-mikroskopicheskaia kharakteriztiki RNK, vydelennoĭ is vysokokontsentrirovannoĭ suspenzii virusa Sendaĭ.