title: Base distribution in the coding and noncoding regions in the rDNA of Lytechinus variegatus.
abstract: The EcoRI restriction endonuclease cleaves rDNA repeat units of the sea urchin Lytechinus variegatus into four fragments. The G + C contents of all four cloned restriction fragments were determined by ispycnic analysis. Electron microscopic denaturation mapping of one of the fragments allowed alignment of the denaturation pattern with the restriction map and correlation with previously reported transcriptional data. From these results the base distribution in the spacer region and regions coding for rRNAs was derived.
mesh_terms: D001482:Base Composition; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D010957:Plasmids; D012335:RNA, Ribosomal / Q000235:genetics*; D012617:Sea Urchins / Q000235:genetics*
chemical_list: D004276:DNA, Satellite; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes