Improved method for virological analysis of food.
A single, simple method for recovering enteroviruses from several different kinds of food, such as ground beef, fish, oysters, and mussels, has been improved. First, sample contamination technique was studied. It appears that virus adsorption occurs at food pH and varies according to the kind of food and the food-virus exchange surface. Second, virus recovery was evaluated. According to the experimental results obtained, we propose the following method. Samples are submerged in 100 ml of demineralized water adjusted to pH 9 with a conductivity of 8,000 mg of NaCl per liter. Then, viruses are concentrated by ultrafiltration or by ultracentrifugation. This method was efficient for virus recovery from the four kinds of food, even in cases of very low contamination.
D000818:Animals; D049872:Bivalvia; D002417:Cattle; D004553:Electric Conductivity; D005399:Fishes; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008460:Meat; D008828:Microbiological Techniques; D010125:Ostreidae; D017955:Poliovirus / Q000302:isolation & purification*; D014461:Ultracentrifugation; D014462:Ultrafiltration