title: Molecular cloning of the Harvey sarcoma virus circular DNA intermediates. II. Further structural analyses.
abstract: Three species of unintegrated supercoiled Harvey sarcoma virus DNA (6.6, 6.0, and 5.4 kilobase pairs) have been molecularly cloned from Harvey sarcoma virus-infected cells. On the basis of restriction enzyme analyses, the 6.6- and 6.0-kilobase pair viral DNAs contain two and one copies, respectively, of a 650-base pair DNA segment which contains sequences present at the 3' and 5' termini of the viral genome. R-loop structures formed between Moloney leukemia virus RNA and the cloned Harvey sarcoma virus DNA indicated that about 500 base pairs of the 650-base pair repeating segment was complementary to the 3' end of the viral RNA. During amplification in the Escherichia coli host, some recombinants containing the 6.6- or the 6.0-kilobase pair Harvey sarcoma virus DNA insert acquired or lost the complete 650-base pair DNA segment. These changes occurred in both recA+ and recA- E. coli.
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics*; D004274:DNA, Recombinant / Q000032:analysis*; D004278:DNA, Superhelical / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D051379:Mice; D008854:Microscopy, Electron; D008979:Moloney murine leukemia virus / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral; D009053:Sarcoma Viruses, Murine / Q000235:genetics*
chemical_list: D004270:DNA, Circular; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D004279:DNA, Viral; D012367:RNA, Viral; D004262:DNA Restriction Enzymes