Ribonuclease H levels in herpes simplex virus-infected cells.
Two forms of ribonuclease H (RNase H) have been identified both in uninfected and Herpes Simplex virus (HSV-)infected BHK cells. Identical RNase H species were detected in control- as well as in infected cells. RNase H I and II have not been found to be associated both with host cell DNA polymerase alpha and beta and HSV-induced DNA polymerase. Infection of BHK cells with HSV type 1 does not lead to a pronounced alteration of RNase H II activity but to an increase (3-fold) of the extractable RNase H I activity. RNase H I activity increases to a maximum between 8-10 hours p.i.; the bulk of HSV-DNA synthesis occurs between 6-8 hours p.i. From these experiments we draw the preliminary conclusion that RNase H I is involved in the degradation of the RNA primer which is covalently linked to newly synthesized HSV-DNA strands.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral / Q000096:biosynthesis; D005033:Ethylmaleimide / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney; D008970:Molecular Weight; D011119:Polynucleotides / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D018139:Simplexvirus / Q000254:growth & development* / Q000378:metabolism; D013379:Substrate Specificity
D004279:DNA, Viral; D007527:Isoenzymes; D011119:Polynucleotides; D012260:Ribonucleases; D005033:Ethylmaleimide