GABA- and glycine-induced Cl- channels in cultured mouse spinal neurons require the same energy to close.
Fluctuation analysis has been used to study the influence of temperature on the conductance and mean open time of Cl- permeable ion channels induced by gamma-aminobutyric acid (GABA) and glycine in the membrane of tissue cultured mouse spinal neurons. The conductance of GABA- and glycine-activated channels increased slightly at higher temperatures (Q10 approximately 1.3). The mean open times of these channels decreased at higher temperatures (Q10 approximately 3). Arrhenius plots for the open times of GABA- and glycine-induced channels were parallel and showed no obvious transition behavior over the temperature range 16-40 degrees C.
D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D004622:Embryo, Mammalian; D005998:Glycine / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D002712:Chlorides; D007473:Ion Channels; D005680:gamma-Aminobutyric Acid; D005998:Glycine