Isolation and characterization of two rubredoxins from Clostridium thermoaceticum.
Two rubredoxins with similar molecular weights (about 6000) have been purified from Clostridium thermoaceticum, a thermophile and strict anaerobe. They exhibit minor differences in several properties like elution pattern from DEAE-cellulose column, isoelectric point, amino acid composition, absorption and EPR spectra and redox potential. Their chemical and physical properties are similar to those of other rubredoxins from anaerobic microorganisms.
D000596:Amino Acids / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D003013:Clostridium / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000032:analysis*; D007501:Iron / Q000032:analysis; D007526:Isoelectric Point; D008970:Molecular Weight; D010084:Oxidation-Reduction; D012416:Rubredoxins / Q000032:analysis* / Q000302:isolation & purification; D013057:Spectrum Analysis
D000596:Amino Acids; D005288:Ferredoxins; D012416:Rubredoxins; D007501:Iron