A modified absorption-reduction method to detect virus-specific hemagglutination inhibiting and neutralizing IgM antibodies.
IgG antibodies are preferentially absorbed by protein A-coupled sepharose beads while most of the IgM and IgA antibodies remain in solution. Even relatively low amounts of virus-specific IgM antibodies can then be detected unequivocally by a decrease of the antibody titer following the treatment with reducing agents. Ethandithiol proved superior to 2-mercaptoethanol in combination with neutralization assays.
D000042:Absorption; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D004770:Enterovirus / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis*; D007158:Immunologic Techniques; D008623:Mercaptoethanol / Q000031:analogs & derivatives / Q000494:pharmacology; D009500:Neutralization Tests; D012412:Rubella virus / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A; D008623:Mercaptoethanol; C031854:1,2-ethanedithiol