title: Diagnosis of eosinophilic granuloma of bone by cytology, histology, and electron microscopy of transcutaneous bone-aspiration biopsy.
abstract: The cytological, histological, and ultrastructural morphology of transcutaneous bone aspirates from five patients with eosinophilic granuloma of bone were studied. Use of this combined approach enabled us to make a definitive diagnosis of eosinophilic granuloma from a small quantity of tissue without having to resort to open biopsy. By light microscopy, eosinophilic granuloma is composed of a polymorphous infiltrate of characteristic lobulated histiocytes, eosinophils, polymorphonuclear leukocytes, and giant cells. Each specimen examined ultrastructurally demonstrated the characteristic Langerhans granule-containing histiocyte. Eosinophilic granuloma is a benign lesion which is readily diagnosed by this technique, and more aggressive diagnostic procedures usually are not warranted.
mesh_terms: D000328:Adult; D001707:Biopsy, Needle; D001842:Bone and Bones / Q000648:ultrastructure*; D002648:Child; D002675:Child, Preschool; D004803:Eosinophilic Granuloma / Q000175:diagnosis / Q000473:pathology*; D005260:Female; D006644:Histiocytes / Q000648:ultrastructure; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008297:Male; D008853:Microscopy; D008854:Microscopy, Electron