Rapid turnover of the histone-ubiquitin conjugate, protein A24.
The specific activity of protein A24 was found to exceed that of the core histones by 2-3 fold following a brief labeling period. Accordingly, the A24 protein was found to be unstable, with a decay half-life of 90 minutes. When decay of the ubiquitin moiety was measured, it was found to turn over more extensively than the H2A moiety.
D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D014452:Ubiquitins
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D009698:Nucleoproteins; D014452:Ubiquitins; C021645:chromatin conjugate protein A24; D008715:Methionine; D008239:Lysine