Immunofluorescence technique for detection of human cytomegalovirus (HCMV) antibodies against HCMV-induced late antigens with elimination of immunoglobulin G-receptor staining.
An immunofluorescence technique is described which permits the elimination of nonspecific cytoplasmic staining from late antigen preparations by using in situ a buffer containing Nonidet P-40.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006531:HEPES / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000187:drug effects / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011971:Receptors, Immunologic; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D006531:HEPES