Visual reading of enzyme immunofluorescence assays for human cytomegalovirus antibodies.
Enzyme immunofluorescence assays for cytomegalovirus antibodies could be read satisfactorily using a light box with ultraviolet illumination. Higher antibody titers were obtained with a fluorogenic substrate than with a color-producing substrate.
D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D013050:Spectrometry, Fluorescence
D000914:Antibodies, Viral; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; C005359:4-methylumbelliferyl phosphate; D006923:Hymecromone