[Ultrastructure of Candida utilis during pulsed carbon feeding and in the presence of different initial concentrations of nitrogen].
The yeast Candida utilis IBPM-Y-405 was cultivated in a chemically defined medium in the conditions of pulse addition of a carbon source. The ultrastructure of the yeast cells was studied at different initial concentrations of a nitrogen source (0.152, 0.088 and 0.044 g of NH4+ per litre of the medium). Morphologically different mitochondria (dense and transparent) were found in one and the same cell at an initial concentration of NH4+ equal to 0.088 g per litre of the medium. When this concentration was equal to 0.044 g per litre of the medium, the number of transparent mitochondria increased, the mitochondria shrank, giant vacuoles comprising regions of the cytoplasm appeared in the cells, as well as large lipid inclusions, the cytoplasm became less thick and contained crystal-like inclusions. All of the aforementioned ultrastructural changes are quite reversible if the initial concentration of the nitrogen source is increased.
D002175:Candida / Q000648:ultrastructure*; D002244:Carbon / Q000378:metabolism*; D002479:Inclusion Bodies / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009584:Nitrogen / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D000644:Quaternary Ammonium Compounds; D002244:Carbon; D009584:Nitrogen
Ul'trastruktura kletok Candida utilis pri impul'snom uglerodnom pitanii i razlichnykh iskhodnykh kontsentratsiiakh azota.