Modulation of the mitotic action of ethylene dibromide.
Refeeding rats treated with a single high dose of ethylene dibromide (1,2-dibromoethane, EDB) induced liver DNA synthesis. The peak of DNA synthesis, as measured by [methyl-3H]thymidine incorporation was attained after 24 h in refed rats and at 48 h in fasted ones. Fasting enhances the EDB action leading to liver cell necrosis, as shown by elevation of serum enzymes' activities, glutamic pyruvic transaminase (GPT) and sorbital dehydrogenase (SDH). A low dose of EDB administered during 2 and 3 weeks slightly enhanced the liver DNA synthesis and elevated the activity of serum enzymes. Phenobarbitone (PB) treatment of rats together with low dose of EDB during 2 weeks prevented the enzyme activity elevation and attenuated the DNA synthesis. Diethyldithiocarbamate (DDC) pretreatment potentiated the DNA synthesis in fed rats after both a small dose of EDB for 2 weeks and after a single high-dose treatment. In DDC pretreated rats, the high single dose of EDB caused biochemical perturbations in serum and liver representative of liver cell necrosis; changes in serum enzymes' activities also were noticed as early as 2 h after EDB toxication. The possible function of modulators on the mitogenic or the necrogenic action of EDB is discussed.
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004050:Ditiocarb / Q000494:pharmacology*; D015946:Ethylene Dibromide / Q000008:administration & dosage / Q000494:pharmacology*; D005215:Fasting; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008938:Mitosis / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013859:Thiocarbamates / Q000494:pharmacology*
D006842:Hydrocarbons, Brominated; D013859:Thiocarbamates; D015946:Ethylene Dibromide; D004050:Ditiocarb; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D002118:Calcium; D010634:Phenobarbital