[Sensitivity of intranuclear glucocorticoid complex from normal rat liver and Zajdela ascites hepatoma to ionic strength and nuclease treatment].
After 30 min of intraperitoneal injection of dexamethasone to adrenectomized rats about 15% of the label are incorporated into liver homogenate and only 1% of the cytosol-bound hormone is detected in the cell nuclei. The binding of the "in vitro" injected hormone by the nuclei does not obey the second-order reactions (the Scatchard plots). This is probably due to the existence of various ancillary mechanisms, which control the translocation of the hormone complex into cell nuclei at the level of cytoplasm and nuclear membranes. DNAase I, micrococcal nuclease and endogenous nucleolysis markedly increase the part of the nuclear hormone complex resistant to 0.4 M NaCl. In hepatocyte nuclei obtained by the collagenase method, the content of the 0.4 M NaCl-resistant receptor complex is also increased. The resistance of 0.4 M NaCl was also found in 80% of the glucocorticoid-insensitive nuclear complex from Zajdela ascite hepatoma cells. The changes in interaction of the hormone-receptor complex with nuclear acceptor sites eventually resulting in impaired sensitivity of host tissues to hormonal control can be due to the damage of chromatin structure induced by different influences and tumour growth.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000494:pharmacology; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D004720:Endonucleases / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008836:Micrococcal Nuclease / Q000494:pharmacology; D009994:Osmolar Concentration; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease
Chuvstvitel'nost' vnutriiadernogo gliukokortikoidnogo kompleksa iz normal'noĭ pecheni krys i astsitnoĭ gepatomy zaĭdela k razlichnoĭ innoĭ sile i nukleaznoĭ obrabotke.