title: The 4-methylumbelliferone sulphate sulphatases of human tears.
abstract: The properties of human tear 4-methylumbelliferone sulphate sulphatase (EC 3.1.6.1) have been investigated. More than 80% of the enzyme activity behaved as the acidic A isoenzyme on isoelectric focusing and DEAE-cellulose ion exchange chromatography. The distribution of enzyme activity in a normal population has been investigated. The least interindividual variation was seen when enzyme activities were calculated as units per mg tears. The alpha-galactosidase, beta-N-acetylglucosaminidase and sulphatase enzyme activities in human tears varied independently of each other. The use of tear sulphatases for the detection of metachromatic leukodystrophy (McKusick 24980) is discussed.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D001192:Arylsulfatases / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000378:metabolism; D007966:Leukodystrophy, Metachromatic / Q000378:metabolism; D013429:Sulfatases / Q000032:analysis / Q000378:metabolism*; D013666:Tears / Q000201:enzymology*; D000519:alpha-Galactosidase / Q000378:metabolism
chemical_list: D006923:Hymecromone; D013429:Sulfatases; C021789:methylumbelliferone sulfatase; D001192:Arylsulfatases; D000519:alpha-Galactosidase; D000118:Acetylglucosaminidase