HgCl2 inhibition of D-glucose transport in jejunal tissue from 2 day and 21 day chicks.
Chick jejunal slices that were exposed to HgCl2 exhibited markedly reduced uptakes of D-glucose. The carrier-mediated (phloridzin inhibitable), but not diffusive, component of uptake was affected by Hg. Within each age group, I50 values (concentration of HgCl2 causing 50% inhibition of mediated transport) for slices (mucosal plus serosal exposure) and sacs (mucosal exposure only) were nearly identical; values for brush border membrane vesicles were slightly lower (increased sensitivity). For each preparation, I50 values for 2 day chicks were consistently lower than corresponding values for 21 day chicks. Taken together, these data indicate that the jejunal brush border membrane is a site of HgCl2 action and that membrane sites in tissue from 2 day chicks are more sensitive to Hg than similar sites in tissue from 21 day chicks.
D000367:Age Factors; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D008628:Mercury / Q000378:metabolism / Q000494:pharmacology*; D010695:Phlorhizin / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013997:Time Factors
D012964:Sodium; D010695:Phlorhizin; D000254:Sodium-Potassium-Exchanging ATPase; D008628:Mercury; D005947:Glucose