Enhanced capacity of DNA repair in human cytomegalovirus-infected cells.
Plaque formation in Vero cells by UV-irradiated herpes simplex virus was enhanced by infection with human cytomegalovirus (HCMV), UV irradiation, or treatment with methylmethanesulfonate. Preinfection of Vero cells with HCMV enhanced reactivation of UV-irradiated herpes simplex virus more significantly than did treatment with UV or methylmethanesulfonate alone. A similar enhancement by HCMV was observed in human embryonic fibroblasts, but not in xeroderma pigmentosum (XP12BE) cells. It was also found that HCMV infection enhanced hydroxyurea-resistant DNA synthesis induced by UV light or methylmethanesulfonate. Alkaline sucrose gradient sedimentation analysis revealed an enhanced rate of synthesis of all size classes of DNA in UV-irradiated HCMV-infected Vero cells. However, HCMV infection did not induce repairable lesions in cellular DNA and did not significantly inhibit host cell DNA synthesis, unlike UV or methylmethanesulfonate. These results indicate that HCMV enhanced DNA repair capacity in the host cells without producing detectable lesions in cellular DNA and without inhibiting DNA synthesis. This repair appeared to be error proof for UV-damaged herpes simplex virus DNA when tested with herpes simplex virus thymidine kinase-negative mutants.
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003586:Cytomegalovirus Infections / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004279:DNA, Viral / Q000528:radiation effects; D006801:Humans; D018139:Simplexvirus / Q000235:genetics; D014466:Ultraviolet Rays; D014779:Virus Replication
D004279:DNA, Viral; D004247:DNA