Kinetics of internalisation and degradation of surface-bound interferon in human lymphoblastoid cells.
The binding of iodinated human interferon-alpha 2 (IFN-alpha 2) was studied on the human T cell line, Molt 4. After its initial binding to cells, the IFN is transferred to a trypsin-resistant compartment before appearing in the medium as TCA-soluble material, while the total cell-associated IFN declines to one-third of its maximum value after 3 h incubation. The Na+/H+ ionophore monensin did not prevent intracellular accumulation of IFN but did completely inhibit its breakdown. We interpret our results as evidence for receptor-mediated internalisation of IFN followed by intracellular breakdown.
D002460:Cell Line; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D008223:Lymphoma; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D013601:T-Lymphocytes / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D007370:Interferon Type I; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D014357:Trypsin