A new approach for assaying LH by using a target cell response.
Replicate cultures of 50000 mouse Leydig cells were used to obtain linear log dose-response curves to LH. This hormone augmented the activity of an endogenous alkaline phosphatase, which was subsequently allowed to act on p-nitrophenyl phosphate, the coloured end-point being measured with a spectrophotometer. The linear portion of the curve extended from 0.2 to 3.2 mi.u. LH/ml.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002478:Cells, Cultured; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology; D007986:Luteinizing Hormone / Q000032:analysis* / Q000494:pharmacology; D008297:Male; D051379:Mice
D007986:Luteinizing Hormone; D000469:Alkaline Phosphatase; D009597:4-Nitrophenylphosphatase