Characterization of responding cells in oxidative mitogen stimulation. I. Ia+ cells and Ly-1+2+ cells are required for the proliferative response.
Addition of anti-Ia sera to cultures of mouse spleen cells stimulated with oxidative mitogens, neuraminidase/galactose oxidase (NaGO) or sodium periodate (NaIO4), inhibits the subsequent proliferative response 30 to 70%. Anti-H-2K or D sera were not specifically inhibitory. Similar inhibition was seen when cells were pretreated with anti-Ia sera and washed before exposure to the mitogenic enzymes. Treatment with anti-Ia serum and complement depletes greater than 89% of the NaGO and the NaIO4 responses but 2% or less of the phytohemagglutinin (PHA) response. The response to NaGO was sensitive to depletion with anti-Thy-1 serum, rabbit anti-mouse brain serum, anti-Ly-1, or anti-Ly-2 serum. Mixtures of Ly-1 and Ly-2-depleted populations did not restore responsiveness. Thus both an Ia+ cell and an Ly-1+2+ T cell are required for [3H]TdR incorporation in response to NaGO treatment.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D005692:Galactose Oxidase / Q000494:pharmacology; D006649:Histocompatibility Antigens; D007106:Immune Sera / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology*; D009439:Neuraminidase / Q000494:pharmacology; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens; D007106:Immune Sera; D008934:Mitogens; D010504:Periodic Acid; D005692:Galactose Oxidase; D009439:Neuraminidase