Molecular analysis of the unc-54 myosin heavy-chain gene of Caenorhabditis elegans.
The properties of a small internal deletion mutant, E675, have been exploited in the molecular cloning of the unc-54 gene. This mutation uniquely identifies unc-54 sequences as molecules of altered length in E675 and provides a genetic and physical marker for the active gene, its messenger RNA and its protein product.
D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005796:Genes; D009154:Mutation; D009218:Myosins / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes; D009218:Myosins