Localization of Na+, K+ -ATPase to the inside of the basolateral cell membranes of epithelial cells of proximal and distal tubules in rabbit kidney.
Cysteine-sensitive alkaline phosphatase and/or ouabain-sensitive Na+, K+ -ATPase were studied by ultrastructure cytochemistry in epithelial cells of proximal and distal kidney tubules. Alkaline phosphatase reactivity was confined to the surface of the microvillous luminal cell membrane of proximal tubule cells, whereas distal tubules and collecting ducts were unreactive. The Na+, K+- ATPase reactivity was localized evenly along the cytoplasmic side of the basolateral cell membrane of cells of proximal and distal tubules and in collecting ducts. In the proximal tubules, where the activity was strongest, the Na+, K+- ATPase deposits were also found in the 10--50 nm gap between the cell membrane and the cisternae of tubulo-cisternal endoplasmic reticulum (TER) underlying a major part of the basolateral cell membrane. The restriction of NA+, K+ -ATPase sites, which are involved in extrusion of Na+ from the cell, to a narrow cytoplasmic compartment located between the cell membrane and the cisternae of TER, is consistent with a transport role for the TER.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D007684:Kidney Tubules / Q000201:enzymology*; D007685:Kidney Tubules, Collecting / Q000201:enzymology; D007686:Kidney Tubules, Distal / Q000201:enzymology* / Q000648:ultrastructure; D007687:Kidney Tubules, Proximal / Q000201:enzymology* / Q000648:ultrastructure; D008871:Microvilli / Q000201:enzymology; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D000254:Sodium-Potassium-Exchanging ATPase