title: A cDNA clone from Zea mays endosperm sucrose synthetase mRNA.
abstract: A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of approximately 20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment.
mesh_terms: D001482:Base Composition; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005632:Fructose / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D003313:Zea mays
chemical_list: D012333:RNA, Messenger; D005632:Fructose; D004247:DNA; D005964:Glucosyltransferases; C022699:sucrose synthase; D004262:DNA Restriction Enzymes