Isolation of spermatozoa in vaginal contents by centrifugation in a colloidal silica gradient.
Human spermatozoa were separated from vaginal contents with epithelial cells by density gradient centrifugation in colloidal silica coated with polyvinylpyrrolidone. In this procedure spermatozoa were collected at a density range of 1.130 - 1.142 g/ml by centrifugation at 35 000 g for 1 h.
D002499:Centrifugation, Density Gradient / Q000379:methods*; D003102:Colloids; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D012822:Silicon Dioxide; D013077:Sperm Head; D013094:Spermatozoa; D014621:Vagina
D003102:Colloids; D012822:Silicon Dioxide