title: Glycoproteins of murine leukemia viruses. III. Glycosylation of env precursor glycoproteins.
abstract: We have compared the glycopeptides obtained after extensive pronase digestion of the env precursors (PrENV proteins) of ecotropic, xenotropic, and dual-tropic murine leukemia viruses. Two glycopeptide size classes, having molecular weights of approximately 2,200 and 1,500, were shown to be associated with the PrENV proteins of all murine leukemia viruses studied. Glycopeptides associated with the env precursors were totally susceptible to endo-beta-N-acetyglucosaminidase H. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of partial endo-beta-N-acetylglucosaminidase H digestion products of the env precursor of dual-tropic mink cell focus-forming virus (MCF 247) revealed the presence of seven bands, suggesting that six glycosylation sites were present on the precursor molecule. The MCF 247 PrENV protein had been previously shown to be accessible to lactoperoxidase-catalyzed radioiodination on the surface of infected cells. The cell surface PrENV molecules had the same electrophoretic mobility as pulse-labeled PrENV protein, and after endo-beta-N-acetylglucosaminidase H treatment a similar shift in electrophoretic mobility was observed for the cell surface PrENV protein and the pulse-labeled precursors, a finding which indicated that the PrENV protein located on the cell surface also possessed only mannose-rich oligosaccharides. These results indicated that the env precursor glycoproteins of dual-tropic viruses had the unusual property of migrating to the cell surface without undergoing the normal oligosaccharide processing and proteolytic cleavage events that had been observed for ecotropic and xenotropic murine leukemia virus glycoproteins.
mesh_terms: D000406:AKR murine leukemia virus / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis*; D009052:Leukemia Virus, Murine / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008907:Mink; D011498:Protein Precursors / Q000032:analysis* / Q000378:metabolism; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D006020:Glycopeptides; D011498:Protein Precursors; D014759:Viral Envelope Proteins; D014764:Viral Proteins