Induction of endogenous murine type C virus by ultraviolet-irradiated herpes simplex virus: effect of metabolic inhibitors.
Ultraviolet-irradiated herpes simplex virus (u.v.-HSV) induced endogenous xenotropic type C virus from AI--2 cells, derived from the BALB/c mouse, as determined by infectious centre focus-forming assay on permissive normal rat kidney (NRK) cells. The number of cells induced to release type C virus by irradiated HSV was dependent on the level of u.v. exposure received by the HSV. Optimal induction occurred when cells were infected with irradiated HSV during their exponential growth phase. Virus induction decreased under conditions of simultaneous cellular exposure to hydroxyurea or actinomycin D, inhibitors of DNA and RNA synthesis, respectively, with actinomycin D having a greater inhibitory effect. This suggests that both DNA and RNA synthesis are required for irradiated HSV induction of murine xenotropic virus. Hydroxyurea decreased induction in the first few hours after infection of A1--2 cells with irradiated HSV, suggesting that the biological events involving DNA synthesis which are required for induction by u.v.-HSV occur shortly after infection.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology*; D051379:Mice; D012334:RNA, Neoplasm / Q000096:biosynthesis; D012190:Retroviridae / Q000187:drug effects / Q000254:growth & development*; D009053:Sarcoma Viruses, Murine; D018139:Simplexvirus / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D003609:Dactinomycin; D006918:Hydroxyurea