Regional chromosome mapping of the human skin type I procollagen gene using adenovirus 12-fragmentation of human-mouse somatic cell hybrids.
Prevous work, using human-mouse somatic cell hybrids, has localized the structural gene for human skin type I procollagen (COL 1) to chromosome 17. One of these hybrids contained only the long arm of human chromosome 17, translocated onto a mouse chromosome, as human chromosomal material. This hybrid was treated with adenovirus 12, and various clones were picked which contained different-sized fragments of human chromosome 17 that were still translocated onto a mouse chromosome. Measurements of these fragments, combined with assays for human COL 1 production and galactose kinase (GAK) activity (also localized on the long arm of human chromosome 17), has allowed us to regionally map the structural gene for human COL 1 to an area just distal to the thymidine kinase (TK) and GAK genes within bands q21 and q22 on human chromosome 17.
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D005690:Galactose; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D051379:Mice; D010770:Phosphotransferases / Q000235:genetics; D011347:Procollagen / Q000235:genetics*; D012867:Skin / Q000378:metabolism; D013937:Thymidine Kinase / Q000235:genetics
D011347:Procollagen; D010770:Phosphotransferases; D013937:Thymidine Kinase; D005690:Galactose