Selective protein transport: identity of the solubilized phosvitin receptor from chicken oocytes.
By two independent methods, the solubilized receptor for phosvitin (PV) has a subunit MW of 116K. Affinity chromatography, showed that only 2 of the more than 25 proteins present in the total detergent solubilized oocyte membrane extract were retained on a PV-agarose column. These proteins of MW of 116K and 100K could be eluted from PV-agarose with free PV. By gel exclusion chromatography, the receptor-125I-PV complexes elute in the void volume of a Biogel A-1.5 column. When these void fractions were assayed gy SDS-PAGE only a single protein of MW of 116K was observed in addition to 125I-PV.
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000032:analysis; D002645:Chickens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004527:Egg Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D017830:Octoxynol; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D011092:Polyethylene Glycols; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D012995:Solubility
D004527:Egg Proteins; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor; D011092:Polyethylene Glycols; D017830:Octoxynol