Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells.
The binding and internalization of 125I-epidermal growth factor (125I-EGF) was studied in cultures of BALB 3T3 cells using a novel method that involved removal of cell-surface hormone by treatment with acetic acid under conditions that did not remove internalized hormone. In control cultures, 125I-EGF initially bound to its receptor on the plasma membrane and then was rapidly internalized. After 30 min, only 15% of the cell-bound hormone remained on the surface. In contrast, cultures treated with dansylcadaverine retained 82% of the cell-bound hormone on the cell surface. We propose that dansylcadaverine inhibits EGF internalization by preventing it from clustering in clathrin-coated pits.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002103:Cadaverine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003619:Dansyl Compounds / Q000494:pharmacology; D003959:Diamines / Q000031:analogs & derivatives*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism
D003619:Dansyl Compounds; D003959:Diamines; D010455:Peptides; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; C008542:monodansylcadaverine; D002103:Cadaverine