Phosphate accumulation by the isolated renal tubule and the effects of parathyroid hormone and cyclic adenosine monophosphate.
The effects of metabolic and transport inhibitors and PTH, cAMP, db-cAMP, and theophylline on Pi uptake by an isolated rat renal tubular preparation has been investigated. Pi uptake by this preparation was a saturable process, was O2- and sodium-dependent, was inactivated by heat=treatment, and was inhibited by dinitrophenol. iodacetate, ouabain, and a decrease in temperature from 37 degrees to 4 degrees C. PTH and cAMP stimulated Pi uptake by energy-dependent and ouabain-inhibitable mechanisms.
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D004136:Dinitrobenzenes / Q000494:pharmacology; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009943:Organophosphorus Compounds / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors
D004136:Dinitrobenzenes; D009943:Organophosphorus Compounds; D010281:Parathyroid Hormone; D010710:Phosphates; D013806:Theophylline; D000242:Cyclic AMP