Freeze-drying of hemoglobin solutions without adjuvant and in presence of glucose, tris, and beta-alanine: a study by electron spin resonance of the oxidized compounds produced.
Hemoglobin cannot be freeze-dried without the presence of protective compounds. Carbohydrates are a well-known example of such compounds, but we have shown that some amine buffer and amino acids are also very effective. The mechanism of action of all these molecules is unknown. We report here experimental data showing that the protective effect is not the result of a direct bond between iron and the protective compound added.
D000042:Absorption; D000409:Alanine; D004578:Electron Spin Resonance Spectroscopy; D005612:Freeze Drying; D005947:Glucose; D006454:Hemoglobins; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism; D012996:Solutions; D014325:Tromethamine; D015091:beta-Alanine
D006454:Hemoglobins; D012996:Solutions; D014325:Tromethamine; D015091:beta-Alanine; D008706:Methemoglobin; D005947:Glucose; D000409:Alanine