Restriction and modification in Bacillus subtilis: identification of a gene in the temperate phage SP beta coding for a BsuR specific modification methyltransferase.
A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperature Bacillus subtilis phage SP beta. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence of active methyltransferase in induced cells leads to modification of BsuR recognition sites in SP beta DNA as well as in heterologous DNA.
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D008242:Lysogeny; D008780:Methyltransferases / Q000235:genetics*; D009154:Mutation; D014466:Ultraviolet Rays; D014775:Virus Activation
D004279:DNA, Viral; C028464:DNA modification methylase BsuR; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C025629:GCAGC-specific type II deoxyribonucleases