Changes in the expression of two Epstein-Barr virus-associated antigens, EBNA and RANA, during the cell cycle of transformed human B lymphoblasts.
Two methods of cell sychronization, density-dependent arrest and double thymidine block, were used to assign two Epstein-Barr virus-associated antigens to different parts of the growth cycle of the human B lymphblastoid cell lines, WI-L2 and Raji. The Epstein-Barr nuclear antigen (EBNA), as detected by anti-complement immunofluorescence, was maximally expressed during early S phase, decreased during the G2 and M phases, and was absent in early G1. In contrast, the rheumatoid arthritis nuclear antigen (RANA), as detected by anti-immunoglobulin immunofluorescence with a prototype serum form a patient with rheumatoid arthritis, was maximally expressed during early G1 phase, progressively decreased during S and early G2, and reappeared during late G2/M.
D000956:Antigens, Viral / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001324:Autoantigens; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans
D000956:Antigens, Viral; D001324:Autoantigens; D019309:Epstein-Barr Virus Nuclear Antigens; C030352:rheumatoid arthritis nuclear antigen