title: Purification of tonin by affinity chromatography.
abstract: Tonin has been purified from rat submaxillary glands. The purification procedure included affinity chromatography on Sepharose 4B coupled to antitonin followed by DEAE chromatography and gel filtration on Sephadex G-100. Homogeneity of the purified enzyme was confirmed by Sephadex G-100 gel filtration, disc electrophoresis, and isoelectric focusing on polyacrylamide gel, immunodiffusion, and immunoelectrophoresis. The tonin was purified 11.5-fold, with 35% recovery. The purified tonin has full enzymatic or immunological activity.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D005779:Immunodiffusion; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013363:Submandibular Gland / Q000032:analysis
chemical_list: D007106:Immune Sera; D007703:Peptidyl-Dipeptidase A