Characterization of X-tropic endogenous retrovirus-specific RNA uninfected tissues and lymphosarcoma of BALB/c mice.
Analysis of endogenous X-tropic BALB/c retrovirus-specific RNAs in a BALB/c mouse tissue containing mostly non-driving cells (liver), in two normal tissues having significant proportions of dividing cells (20-h regenerating liver and 12-day embryo) and in cells of a lymphosarcoma of BALB/c mice were carried out by determining the extent to which the RNAs from these tissues hybridized to the virus 3H-cDNA probe and by their relative sedimentation values in a sucrose gradient. RNAs from 20-h regenerating liver, 12-day embryo and lymphosarcoma, each containing a significant proportion of proliferating cells, showed 8 to 21 % higher hybridization values than normal liver RNA. Differences in the exact size classes of virus-specific RNAs and, in their relative proportions were found to exist in the four different tissue types examined and no correlation between a specific RNA size-profile and the proliferating activity of a tissue could be detected.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004622:Embryo, Mammalian / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification*; D012190:Retroviridae; D012513:Sarcoma, Experimental / Q000382:microbiology*
D012367:RNA, Viral