Ascorbic acid inhibition of Na,K-adenosine triphosphatase of rat forebrain without peroxidation of membrane lipids.
Using reagents and membrane preparations from which contamination had been carefully removed, we found that ascorbic acid inhibited rat brain Na,K-ATPase without causing lipid peroxidation, unlike the conventional belief; the inhibition was prevented by catecholamines and EGTA. Ascorbic acid radicals, instead of active oxygen, may play a role in the inhibition.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D003600:Cytosol / Q000187:drug effects; D004298:Dopamine / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013574:Synaptosomes / Q000187:drug effects
D008054:Lipid Peroxides; D008563:Membrane Lipids; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D001205:Ascorbic Acid; D004298:Dopamine