Inhibitory effect of 1-beta-D-arabinofuranosylthymine on synthesis of Epstein-Barr virus.
The effect of 1-beta-D-arabinofuranosylthymine (ara-T) on cell growth and synthesis of Epstein-Barr virus (EBV) in human lymphoblastoid cell lines was determined. The growth of P3HR-1 cells was not inhibited by 1 microgram of the drug per ml; however, infectious virus production was strongly inhibited and was accompanied by decreased expression of early antigen (EA) and viral capsid antigen (VCA). The ability of 12-O-tetradecanoylphorbol-13-acetate or n-butyric acid to induce synthesis of VCA, but not EA, in P3HR-1 cells was inhibited by ara-T. Similarly, VCA synthesis but not EA synthesis was inhibited by ara-T in Jijoye cells superinfected with the P3HR-1 strain of EBV. The results suggest that ara-T has a specific inhibitory action against EBV replication.
D000956:Antigens, Viral; D000998:Antiviral Agents / Q000494:pharmacology*; D001087:Arabinonucleosides / Q000494:pharmacology*; D036022:Capsid Proteins; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D006801:Humans; D013936:Thymidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D000998:Antiviral Agents; D001087:Arabinonucleosides; D036022:Capsid Proteins; C010032:thymine arabinoside; D013936:Thymidine