Non-specific cyclic nucleotide binding in leukemic leukocytes.
Extracts of leukemic lymphocytes and leukemic myelomonocytic cells bind significantly more cAMP/mg protein than do normal cells. The binding of cAMP in leukemic cells is non-specific since the binders are unable to effectively discriminate between cAMP and cGMP in contrast to normal cells. When normal lymphocytes are activated in vitro with mitogens, their cAMP-binding capacity is increased, but the binding retains the specificity of normal cells. Thus in malignantly transformed leukocytes, cAMP-binding is characterized by a high degree of non-specificity, compared with the binding in resting or proliferating normal leukocytes.
D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D011485:Protein Binding
D000242:Cyclic AMP; D006152:Cyclic GMP