title: Adenosine 3',5'-monophosphate (cAMP)-binding proteins and cAMP-dependent protein kinases in bovine adrenal cortical cell plasma membrane.
abstract: A cAMP-binding protein and cAMP-dependent protein kinase have been identified and partially characterized in bovine adrenocortical plasma membranes. [3H]cAMP binding to the plasma membrane preparation demonstrates both high (Ka = 4.2 X 10(9) M-1) and low (Ka = 1.3 X 10(8) M-1) affinity binding sites. cAMP-dependent protein kinase was demonstrated using both bovine histone and endogenous substrates. The detergent-dependent solubilization characteristics of this cAMP-dependent protein kinase indicate that it is an integral membrane protein.
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D006656:Protamine Kinase / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D011494:Protein Kinases; D006656:Protamine Kinase