Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel.
Ready-for-use reversed-phase high-performance thin-layer chromatographic plates were used for the separation of Dns-amino acids. The development was performed by using methanol-2% acetic acid (75.25) or methanol-0.01 M Na2HPO4 (75:25). Reversed-phase plates were used also for adsorption chromatography using a non-aqueous solvent system such as benzene-chloroform-acetic acid (50:48:2) or n-heptane-ethyl acetate-acetic acid (65:33:2). Good separations were achieved by using both principles in the two-dimensional arrangement.
D000085:Acetates; D000327:Adsorption; D000596:Amino Acids / Q000032:analysis*; D001554:Benzene; D002725:Chloroform; D002855:Chromatography, Thin Layer / Q000379:methods*; D003619:Dansyl Compounds / Q000032:analysis*; D005782:Gels; D006536:Heptanes; D007202:Indicators and Reagents; D000432:Methanol; D010710:Phosphates; D012822:Silicon Dioxide; D012964:Sodium
D000085:Acetates; D000596:Amino Acids; D003619:Dansyl Compounds; D005782:Gels; D006536:Heptanes; D007202:Indicators and Reagents; D010710:Phosphates; D012822:Silicon Dioxide; D002725:Chloroform; D012964:Sodium; D001554:Benzene; D000432:Methanol