title: Phosphate binding to liver alcohol dehydrogenase studied by the rate of alkylation with affinity labels.
abstract: In this work we report that phosphate anions interact with the anion binding site of alcohol dehydrogenase from horse liver. In protection experiments against the two affinity labels, iodoacetic acid and bromo-imidazolylpropionic acid, the dissociation constant for the enzyme-phosphate complex at pH 7.0 is, based on total phosphate, found to be 20 +/- 5 mM. The 1,4-piperazinediethanesulfonate anion has a lower affinity for the anion binding site, the dissociation at pH 7.0 being 130 +/- 20 mM. The anion-independent dissociation constants for the reversible enzyme-affinity label complexes are at pH 7.0, 1.35 +/- 0.2 mM for iodoacetic acid and 0.39 +/- 0.05 mM for bromo-imidazolylpropionic acid. These findings have important implications with respect to past and future work on this well known enzyme.
mesh_terms: D000345:Affinity Labels / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000476:Alkanesulfonates / Q000494:pharmacology; D017738:Alkanesulfonic Acids; D000478:Alkylation; D000818:Animals; D001665:Binding Sites; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010710:Phosphates / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology
chemical_list: D000345:Affinity Labels; D000476:Alkanesulfonates; D017738:Alkanesulfonic Acids; D007093:Imidazoles; D007461:Iodoacetates; D010710:Phosphates; D010879:Piperazines; C000314:alpha-bromo-beta-(5-imidazolyl)propionic acid; D000429:Alcohol Oxidoreductases; C008916:piperazine-N,N'-bis(2-ethanesulfonic acid)