title: Studies on the induction of aryl hydrocarbon(benzo[a]pyrene) hydroxylase in Neurospora crassa, and its suppression by sodium selenite.
abstract: Six fungal species were grown in the presence of benzo[a]pyrene (BP); four showed benzo[a]pyrene hydroxylase (aryl hydrocarbon hydroxylase, AHH) activity. Penicillium sp. and Neurospora crassa metabolized BP to a limited extent. N. crassa AHH activity was induced by BP, the major product of metabolism being 3-hydroxy-BP. Both induction of AHH activity and metabolism of BP were suppressed by sodium selenite in the growth medium. Two polypeptides, unique to BP-grown cells, were revealed by two-dimensional electrophoretic separation of [35S]methionine-labelled proteins in N. crassa cell extracts. In selenium-grown cells the synthesis of BP-specific polypeptides was severely inhibited.
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000494:pharmacology; D001579:Benzopyrene Hydroxylase / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005656:Fungal Proteins / Q000096:biosynthesis; D005658:Fungi / Q000378:metabolism; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000187:drug effects / Q000378:metabolism*; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D005656:Fungal Proteins; D001564:Benzo(a)pyrene; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D020887:Selenious Acid; D012643:Selenium