Uniparental propagation of mitochondrial DNA in mouse-human cell hybrids.
The retention of the two parental mitochondrial DNAs has been investigated in a large number of mouse-human cell hybrids segregating either mouse or human chromosomes, by using a highly sensitive and specific method for detection of the DNA; the results have been correlated with the karyotype and isozyme marker pattern in the same hybrid lines. In all the hybrids examined, a consistent pattern was observed for the type of mitochondrial DNA retained: the mitochondrial DNA of the parent whose chromosomes were segregated from the nucleus was undetectable or present in marginal amounts. This was true also of hybrids containing a complete set of the segregating chromosomes in the total or a large fraction of the cell population.
D000818:Animals; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis*; D007527:Isoenzymes / Q000235:genetics; D007621:Karyotyping; D051379:Mice; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D004272:DNA, Mitochondrial; D005819:Genetic Markers; D007527:Isoenzymes; D004262:DNA Restriction Enzymes