Alteration in enzyme activities of de novo phosphatidylcholine biosynthesis in rat liver by treatment with typical inducers of microsomal drug-metabolizing system.
1. The activities of choline kinase, ethanolamine kinase, cholinephosphate cytidylytransferase(s) and cholinephosphotransferase were compared in the liver subcellular fractions after the treatment of rats for two successive days with phenobarbital, 3-methylcholanthrene and polychlorinated biphenyls. 2. The administration of phenobarbital resulted in a significant decrease in choline kinase activity while not affecting ethanolamine kinase activity. Both 3-methylcholanthrene and polychlorinated biphenyls caused considerable enhancement of choline kinase activity concomitantly with ethanolamine kinase activity. 3. The activity of cytosolic cytidylytransferase was not affected by any of the inducers while the microsomal activity was significantly depressed by the administration of either phenobarbital or polychlorinated biphenyls. 4. The activity of microsomal cholinephosphotransferase decreased significantly after the treatment with both 3-methylcholanthrene and polychlorinated biphenyls and increased slightly after phenobarbital administration. 5. The observed opposite effects of phenobarbital and 3-methylcholanthrene on the enzymes in de novo phosphatidylcholine synthesis indicate that there exist a possible relation between induction of microsomal drug-metabolizing system and modulation of phosphatidylcholine biosynthesis in animal liver.
D000818:Animals; D002797:Choline Kinase / Q000096:biosynthesis; D019916:Choline-Phosphate Cytidylyltransferase; D003566:Cytidine Diphosphate Choline / Q000096:biosynthesis; D002798:Diacylglycerol Cholinephosphotransferase / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D004983:Ethanolamines / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology; D009713:Nucleotidyltransferases / Q000096:biosynthesis; D010634:Phenobarbital / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000096:biosynthesis; D017853:Phosphotransferases (Alcohol Group Acceptor); D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D051381:Rats
D004983:Ethanolamines; D010713:Phosphatidylcholines; D003566:Cytidine Diphosphate Choline; D008748:Methylcholanthrene; D011078:Polychlorinated Biphenyls; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D002797:Choline Kinase; C020125:ethanolamine kinase; D009713:Nucleotidyltransferases; D019916:Choline-Phosphate Cytidylyltransferase; D002798:Diacylglycerol Cholinephosphotransferase; D010634:Phenobarbital