Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid:deoxyribonucleic acid hybridization.
A method for typing clinical isolates of herpes simplex virus was developed. It utilizes hybridization between unlabeled deoxyribonucleic acid from infected cultures and tritium-labeled virus deoxyribonucleic acid, and it can be completed within a day using a single roller-tube culture of the clinical isolated. The data obtained are inherently quantitative, and the method yields unequivocal identification and typing. Thirty-nine coded clinical isolates were all correctly typed by this method.
D004279:DNA, Viral / Q000032:analysis*; D006561:Herpes Simplex / Q000175:diagnosis; D006801:Humans; D008722:Methods; D009500:Neutralization Tests; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D018139:Simplexvirus / Q000145:classification*; D013010:Sonication
D004279:DNA, Viral