Calcitonin and 1,25-dihydroxyvitamin D3 receptors in human breast cancer cell lines.
Five human breast cancer cell lines (MCF 7, T 47D, BT 20, MDA 157, and MDA 231) and a human breast epithelial cell line (HBL 100) have been found to contain specific high-affinity receptors for 1,25-dihydroxyvitamin D3, Kd values ranged from 0.6 to 2.0 X 10(-11) M and receptor concentration from 31 to 150 fmol/mg cytosol protein. Two of the breast cancer lines (MCF 7 and T 47D) contain specific high-affinity receptors for calcitonin and a calcitonin-responsive adenylate cyclase, which have been characterized with the aid of salmon, eel, and human calcitonins and in several substituted analogues of human calcitonin. The 1,25-dihydroxyvitamin D3 receptor may reflect a normal property of the breast cell. Breast cancer cell lines provide a useful source of 1,25-dihydroxyvitamin D3 receptors. Their coexistence with a calcitonin receptor and biological response in some breast cancers offers the opportunity to investigate new aspects of breast cancer endocrinology.
D000262:Adenylyl Cyclases / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D002116:Calcitonin / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D004100:Dihydroxycholecalciferols / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006801:Humans; D006887:Hydroxycholecalciferols / Q000378:metabolism*; D018003:Receptors, Calcitonin; D018167:Receptors, Calcitriol; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013329:Structure-Activity Relationship
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D018003:Receptors, Calcitonin; D018167:Receptors, Calcitriol; D011956:Receptors, Cell Surface; D011987:Receptors, Steroid; D002116:Calcitonin; D000262:Adenylyl Cyclases