[DNA-methylase from Arthrobacter luteus screens DNA from the action of site-specific endonuclease Alu I].
DNA-methylase was isolated from a cell extract of A. luteus and partially purified by chromatography on phosphocellulose. The purified enzyme methylates DNA of phage lambda and plasmids pBr322, thus making them resistant to a subsequent action of endonuclease Alu I. It has been shown that cytosine is the object of methylation within DNA. This modification does not screen DNA from the action of site-specific endonucleases Sal I, Bam HI, EcoR I, EcoR II, Xho I and Xho II. It has been experimentally demonstrated that the isolated methylase is site-specific and identifies in the DNA the nucleotide sequence 5'-AGCT-3', by methylating cytosine in the DNA.
D001173:Arthrobacter / Q000201:enzymology*; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases / Q000378:metabolism*; D005095:Exoribonucleases; D007700:Kinetics; D008780:Methyltransferases / Q000378:metabolism*; D010957:Plasmids; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
D004247:DNA; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D005092:Exonucleases; D005095:Exoribonucleases; D012260:Ribonucleases; C022535:exoribonuclease II
DNK-metilaza iz Arthrobacter luteus zashchishchaet DNK ot deĭstviia saĭtspetsificheskoĭ éndonukleazy Alu I.