The modified nucleotide constituents of human prostatic cancer cell (MA-160) poly(A)-containing RNA.
Cytoplasmic poly A(+) RNA from human prostatic cancer cells grown in the presence of 32P was isolated by affinity chromatography on columns of oligo(dT)-cellulose. The RNA was digested with RNAase T2 and the products of digestion were fractionated by two-dimensional electrophoresis. The resulting autoradiograms revealed the presence of many different cap groups as well as two internal modified nucleotide components. 19 different type 1 and type 2 'cap' groups were identified. The internal modified nucleotides were N6-methyl adenosine and a 2'-O-methyl nucleotide possessing an unusual modified base.
D002460:Cell Line; D002855:Chromatography, Thin Layer; D003593:Cytoplasm / Q000032:analysis; D004720:Endonucleases; D004722:Endoribonucleases; D006801:Humans; D008297:Male; D010727:Phosphoric Diester Hydrolases; D011061:Poly A / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000032:analysis*; D012315:RNA Caps / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis*
D012315:RNA Caps; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012265:Ribonucleotides; D011061:Poly A; D004720:Endonucleases; D004722:Endoribonucleases; D012260:Ribonucleases; C022543:ribonuclease T(2); D010727:Phosphoric Diester Hydrolases