Investigation of immunoperoxidase-labelled rotavirus in tissue culture by light and electron microscopy.
A tissue culture-adapted strain of bovine rotavirus, grown in calf kidney monolayers, has been examined by light and electron microscopy after immunoperoxidase labelling. Some of the characteristic problems associated with pre-embedding methods have been demonstrated. Preparative techniques involving pretreatment of infected cells with a detergent followed by a detergent/fixative combination have enabled virus antigen to be labelled while maintaining satisfactory ultrastructural preservation.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D002478:Cells, Cultured; D003593:Cytoplasm / Q000276:immunology; D003902:Detergents / Q000494:pharmacology; D005404:Fixatives; D007124:Immunoenzyme Techniques; D008828:Microbiological Techniques; D012087:Reoviridae / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology* / Q000648:ultrastructure
D000956:Antigens, Viral; D003902:Detergents; D005404:Fixatives