Virus RNA species in kirsten murine sarcoma virus-transformed mink cells.
The nuclear and cytoplasmic RNAs from Kirsten murine sarcoma virus (KiMuSV)-transformed non-producer mink cells were studied for the species of virus-specific RNA by fractionation in agarose gels, transfer to diazotized aminophenylthioether paper and hybridization to complementary DNA probe. In both nuclei and cytoplasm, only genome-length KiMuSV-specific RNA was detected. No subgenomic virus RNA species was detected in poly(A+) or poly(A-) RNA fractions. The same observations were made in KiMuSV-transformed mink cells superinfected with feline leukaemia viruses. The significance of these findings is discussed.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002472:Cell Transformation, Viral; D003593:Cytoplasm / Q000032:analysis; D005814:Genes, Viral; D007708:Kirsten murine sarcoma virus / Q000032:analysis / Q000254:growth & development / Q000502:physiology*; D008907:Mink; D012367:RNA, Viral / Q000032:analysis*; D009053:Sarcoma Viruses, Murine / Q000502:physiology*
D012367:RNA, Viral