Fusion of the promoter region of rRNA operon rrnB to lac Z gene.
A Lambda phage was constructed in which the structural gene for beta galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon beta galactosidase synthesis in vitro is repressed by ppGpp. Repression of beta galactosidase synthesis by cAMP is reported.
D010582:Bacteriophage lambda / Q000187:drug effects / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005696:Galactosidases / Q000096:biosynthesis*; D005796:Genes / Q000187:drug effects; D006159:Guanosine Tetraphosphate / Q000494:pharmacology; D008854:Microscopy, Electron; D009876:Operon; D001616:beta-Galactosidase / Q000096:biosynthesis*
D004279:DNA, Viral; D006159:Guanosine Tetraphosphate; D004262:DNA Restriction Enzymes; D005696:Galactosidases; D001616:beta-Galactosidase