Molecular weight of detergent-solubilized prostaglandin-F2 alpha receptor from bovine corpora lutea.
A prostaglandin F2 alpha receptor localized in plasma membranes of bovine corpus luteum cells was solubilized by treatment with Triton X-100. Sepharose chromatographies of ([3H]prostaglandin F2 alpha)-receptor complex gave a Stokes' radius of 630 nm. In the absence of detergent, aggregated forms of the receptor appeared. Sedimentation experiments of solubilized receptor in sucrose/H2O and sucrose/2H2O density gradients gave the following values: sedimentation coefficient (S20, w) 4.6 S; partial specific volume (VB) 0.78 cm3/g and frictional ratio (f/fo) 1.6. Based on the sedimentation coefficient and the Stokes' radius and assuming that the receptor is a non-glycosylated protein the molar mass of the receptor-(Triton X-100) complex was 144000 g/mol. The VB value indicated that ca. 26% of the weight represented bound detergent and that the molecular weight of the prostaglandin F2 alpha receptor is approximately 107000.
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D003338:Corpus Luteum / Q000032:analysis*; D005260:Female; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D011460:Prostaglandins F / Q000378:metabolism; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000302:isolation & purification; D011982:Receptors, Prostaglandin / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility
D011460:Prostaglandins F; D011956:Receptors, Cell Surface; D011982:Receptors, Prostaglandin; D011092:Polyethylene Glycols; D017830:Octoxynol