Extracellular matrix proteins characterize human tumor cell lines.
Extracellular matrix proteins synthesized and secreted by adherent human tumor cell lines were analyzed using metabolic labelling with glycine and proline in the presence of ascorbate, polypeptide analysis and polyacrylamide gel electrophoresis, affinity chromatography, collagenase digestion, and immunofluorescence staining. The results showed a characteristic pattern of matrix proteins for each tumor cell type. Tumor cell lines of mesenchymal origin produced mostly interstitial types (I and II) of collagen and fibronectin. Carcinoma cell lines secreted only basement membrane proteins, type IV collagen, laminin and fibronectin, but not interstitial collagen. A melanoma and a rhabdomyosarcoma cell line produced type V of procollagen that has not previously been described in cell culture. Neuroblastoma cells were shown to be phenotypically heterogeneous also with respect to matrix protein production. We propose that the analysis of extracellular matrix proteins may serve as an adjunct in the classification of human tumors.
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D003012:Microbial Collagenase / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000032:analysis
D009363:Neoplasm Proteins; D003012:Microbial Collagenase