title: Hydrophobic residues involved in the interaction between protomers of the bovine growth hormone dimer. Methionine and tyrosine residues.
abstract: The bovine growth hormone dimeric form covalently stabilized by cross-linking with dimethyl suberimidate (DMS) and the hormone modified by DMS without forming covalent links with other hormone molecules (DMS-bGH) were oxidized with chloramine-T at molar-ratios of 2 and 50 with respect to methionine content. The extent of oxidation undergone by each methionine residue, estimated on the purified tryptic peptides, closely resembled that obtained for the native hormone, thus suggesting that methionine residues are not involved in the protomers interaction area. Evaluation of the reactivity of tyrosine residues toward tetranitromethane indicated that, in both the covalent dimer and DMS-bGH, tyrosine residues 35, 174 and 142 are the more susceptible to undergo reaction. Net charges can be induced in the iodotyrosine residues in the iodinated hormone, by setting the pH at 10.5. At this pH, dissociation of a fraction of uniformly iodinated hormone was observed in the derivatives containing 2 or more iodine atoms, indicating that tyrosine residues might integrate the contact area between protomers.
mesh_terms: D002700:Chloramines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004347:Drug Interactions; D013006:Growth Hormone / Q000378:metabolism*; D008697:Methane / Q000031:analogs & derivatives*; D013329:Structure-Activity Relationship; D013774:Tetranitromethane / Q000378:metabolism*; D014105:Tosyl Compounds; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D002700:Chloramines; D014105:Tosyl Compounds; C016300:chloramine-T; D014443:Tyrosine; D013006:Growth Hormone; D013774:Tetranitromethane; D008697:Methane