ATP prevents both hydroperoxide-induced hydrolysis of pyridine nucleotides and release of calcium in rat liver mitochondria.
The mechanism of the hydroperoxide-induced release of calcium from rat liver mitochondria was investigated. In calcium-loaded mitochondria both the hydroperoxide-induced hydrolysis of pyridine nucleotides and the release of calcium is inhibited by intramitochondrial ATP. In submitochondrial particles, in the absence of ATP a hydrolytic product of NAD+, probably ADP-ribose, binds covalently to a protein of the inner mitochondrial membrane. It is proposed that a compound deriving from NAD(P)+ is an important factor in the calcium release mechanism in rat liver mitochondria.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006868:Hydrolysis; D008565:Membrane Proteins; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009244:NAD+ Nucleosidase / Q000037:antagonists & inhibitors; D009249:NADP / Q000378:metabolism*; D010545:Peroxides / Q000494:pharmacology*; D051381:Rats; D013367:Submitochondrial Particles / Q000201:enzymology; D020122:tert-Butylhydroperoxide
D008565:Membrane Proteins; D010545:Peroxides; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D020122:tert-Butylhydroperoxide; D009244:NAD+ Nucleosidase; D002118:Calcium