Fine structure mapping of an avian tumor virus RNA by immunoelectron microscopy.
The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5'-phosphate (anti-pm 7G), 81% of 106 molecules examined were found to have antibody at one terminus, in agreement with the presence of a pm 7G cap in ASV-RNA. Hapten inhibition by pm 7G could be demonstrated. Experiments with anti-A and with anti-poly A gave results consistent with the known structure of ASV-RNA, in particular the presence of a 3' poly A tail. These studies illustrate the feasibility of using anti-nucleotide antibodies in a combined immunochemical and electron microscopic study of the fine structure of nucleic acids.
D001355:Alpharetrovirus / Q000032:analysis*; D000906:Antibodies; D006241:Haptens; D008854:Microscopy, Electron / Q000379:methods; D008970:Molecular Weight; D012367:RNA, Viral; D011863:Radioimmunoassay
D000906:Antibodies; D006241:Haptens; D012367:RNA, Viral