Requirements for growth of neurites from embryonic chick cerebellum in culture.
Embryonic chick cerebella were cut into pieces and cultured under various conditions. Neurites were extended actively to collagen gel for a week or so and then degenerated. The neurite growth for the first two days was similar, also in the serum-free medium. Among 15 substances tested, insulin and cyclic AMP facilitated the growth.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002531:Cerebellum / Q000166:cytology*; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D000242:Cyclic AMP / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology; D005728:Ganglia, Sympathetic / Q000166:cytology; D007328:Insulin / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects; D009474:Neurons / Q000166:cytology
D003470:Culture Media; D007328:Insulin; D009414:Nerve Growth Factors; D000242:Cyclic AMP