The redistribution of membrane surface immunoglobulin induces the rearrangement of some membrane integral proteins.
We investigated whether the redistribution of surface membrane receptors is associated with rearrangement of integral membrane proteins. Using a newly developed process, which combines histochemical analysis with an immunofluorescence or immuno-electron microscopy-staining technique, we studied the redistribution of two membrane-bound enzymes, 5'-nucleotidase and ATPase, on mouse splenic lymphocytes and B lymphoma cells induced by anti-mouse immunoglobulin antibodies. Labeling and capping of the membrane surface immunoglobulin induced a similar rearrangement of both 5'-nucleotidase and ATPase from uniform distribution at 4 degrees C into 'patches' and caps at 37 degrees C.
D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D007152:Immunologic Capping; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008223:Lymphoma / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology; D009708:Nucleotidases / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*
D008565:Membrane Proteins; D011947:Receptors, Antigen, B-Cell; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases