Isolation and characterization of the complete chicken beta-globin gene region: frequent deletion of the adult beta-globin genes in lambda.
A library of bacteriophage lambda clones containing chicken chromosomal DNA was screened, using the adult beta-globin cDNA plasmid pHb 1001 as a probe. Sixteen overlapping clones were isolated containing 35 kilobase pairs (kbp) of chicken DNA. Characterization of these clones revealed four beta-like globin genes, some genomically repeated sequences, but no pseudo-genes. The four beta-like genes have an average intergenic distance of less than half of that found for the mammalian beta-like globin gene clusters so far characterized. The overall features of the map were confirmed by genomic Southern analysis. Frequent deletions were shown to occur between the various beta-like globin genes during phage propagation. The presumptive hatching gene in particular was always associated with abnormal lambda clones although we were able to find one such clone that did contain a normal copy of the hatching gene itself. Probably such deletions explain the failure to recover this gene in previous attempts.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D002645:Chickens; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D005914:Globins / Q000235:genetics*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004274:DNA, Recombinant; D005914:Globins; D004262:DNA Restriction Enzymes