title: A human liposarcoma cell line producing hyaluronic acid.
abstract: A human liposarcoma cell line COLO 222, derived from a primary tumor in a 62-year-old male, elaborates hyaluronic acid. COLO 222 is characterized on the basis of histochemical, ultramorphological, and cytogenetic properties, along with isozyme phenotype and cell products. A chromosome mode of 53 predominates and unique Giemsa-banded marker chromosomes are identified. An autochthonous lymphoid cell line, COLO 143v, was established after the addition of exogenous Epstein-Barr virus. Cytogenetic analysis of Colo 143v is consistent with a normal male karyotype. COLO 143v possesses B-cell characteristics. This autochthonous system had been used for immunological studies and cytotoxicity assays.
mesh_terms: D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D003602:Cytotoxicity, Immunologic; D004854:Herpesvirus 4, Human; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007527:Isoenzymes / Q000378:metabolism; D008080:Liposarcoma / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged
chemical_list: D007527:Isoenzymes; D006820:Hyaluronic Acid