[Phenomenon of intestinal microfloral increase after stopping the use of poorly adsorbed antibiotics].
The results of studying intestine microflora in conventional mice (CBA X C57 BL/F1) during enteral treatment and after discontinuation of low-adsorbing antibiotics (gentamicin, restomycin and nystatin) are presented. The average daily doses of the antibiotics were 2.4, 3 mg and 110000 units respectively. For determination of the intestine microflora composition various media were used. It was found that a 7-day exposure of the mice to the above antibiotic combination resulted in total decontamination of their intestine. After discontinuation of the antibiotic there was a marked increase in the number of Coli bacteria, i. e. 10(7) times higher than the initial level. The number of other microorganisms returned to normal without any significant increase. It was shown that when the normal Coli microflore of the mice contained equal numbers of lactose-positive and lactose-negative strains, the increase after discontinuation of the antibiotic was mainly due to the lactose-positive organisms. Reduction of the initial quantitative and qualitative composition of the Coli flora was observed 7--8 days after discontinuation of the antibiotic. The analysis of the experimental and literature data demonstrated that the marked increase in the number of the Coli forms in the intestine microflora resulted from discontinuation of antibiotic. Its universal character, connection with antagonistic relations of some microorganisms, the role of exogenic contamination and means for its decreasing are discussed.
D000818:Animals; D005243:Feces / Q000382:microbiology; D005839:Gentamicins / Q000008:administration & dosage*; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009761:Nystatin / Q000008:administration & dosage*; D012310:Ristocetin / Q000008:administration & dosage*; D013997:Time Factors
D005839:Gentamicins; D009761:Nystatin; D012310:Ristocetin
Fenomen vozrastaniia mikroflory kishechnika posle otmeny maloadsorbiruemykh antibiotikov.