Complete in vitro DNA replication of SV40 chromatin in digitonin-treated permeable cells.
A permeable cell system has been developed by treatment with digitonin for studying in vitro DNA replication of chromatin. DNA replication of simian virus 40 nucleoprotein complexes (SV40 chromatin) in digitonin-treated permeable cells was analyzed by electrophoresis in agarose-gel. Autoradiography of the agarose-gel revealed that [32P]dCTP was incorporated in SV40 DNA I, II and replicating intermediates. The time course of the incorporation indicated the complete replication of SV40 DNA and chromatin with a full number of nucleosomes. The digitonin-treated permeable cell system will serve as a useful system for studying in vitro DNA replication of chromatin.
D000818:Animals; D002843:Chromatin / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects*; D004072:Digitonin / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D000882:Haplorhini; D010539:Permeability; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects*
D002843:Chromatin; D004072:Digitonin