Rotavirus concentration from cell culture harvests: trypsin treatment followed by hydroextraction.
An 80- to 150-ml amount of calf or simian rotavirus-containing cell culture harvests of MA-104 cells were treated with 50 microgram of trypsin per ml and hydroextracted overnight (4 degrees C) with polyethylene glycol 6,000. The concentrate was resuspended in 8 to 10 ml of tryptose phosphate broth and plaque assayed. Between 85 and 97% of the input virus could be recovered with a concentration of up to 15-fold.
D002460:Cell Line; D011092:Polyethylene Glycols; D012087:Reoviridae / Q000302:isolation & purification*; D012401:Rotavirus / Q000254:growth & development / Q000302:isolation & purification*; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay; D014776:Virus Cultivation / Q000379:methods*
D011092:Polyethylene Glycols; D014357:Trypsin