Resolution, reconstitution and kinetics of the primary action of a hormone receptor.
The beta-adrenergic receptor (R) and the GTP binding protein (G) with which the receptor interacts were obtained in separate soluble fractions. Their recombination produced a hormone responsive system. In the presence of hormone the encounter of R with G in the reconstituted membrane occurs much faster than the subsequent R-induced activation of G.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D004910:Erythrocyte Membrane / Q000378:metabolism; D019204:GTP-Binding Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012995:Solubility; D014422:Turkeys
D002352:Carrier Proteins; D008565:Membrane Proteins; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D006165:Guanylyl Imidodiphosphate; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases