Platelet-derived growth factor enhances granulosa cell luteinizing hormone receptor induction by follicle-stimulating hormone and serum.
Platelet-derived growth factor potentiated FSH-dependent LH receptor induction in serum-free and serum-containing cultures of rat granulosa cells. Levels of LH receptor binding comparable to pre-ovulatory levels could be achieved in vitro using a combination of FSH, platelet-derived growth factor, and serum. Receptor sites induced under these conditions were capable of mediating an hCG-stimulated increase in progestin secretion. Our results in vitro suggest a possible role for platelet-derived growth factor or related compounds in granulosa cell differentiation in vivo.
D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D011974:Receptors, LH
D003470:Culture Media; D006133:Growth Substances; D008934:Mitogens; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D004054:Diethylstilbestrol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone