Insertion of a new gene of viral origin into bone marrow cells of mice.
DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk+ mouse L cells to a tk++ status in vitro using methotrexate as a selective agent. HSVtk DNA was also used to transform mouse bone marrow cells in vitro. Transformed marrow cells injected into irradiated and methotrexate-treated recipient mice gave rise to proliferating cells which in some cases dominated the marrow population and which contained HSVtk gene sequences.
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D016026:Bone Marrow Transplantation; D004279:DNA, Viral / Q000032:analysis; D004351:Drug Resistance; D005814:Genes, Viral; D007739:L Cells; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D013937:Thymidine Kinase / Q000235:genetics*; D014170:Transformation, Genetic
D004279:DNA, Viral; D013937:Thymidine Kinase; D008727:Methotrexate