title: Complete nucleotide sequence of a cloned chicken alpha-globin cDNA.
abstract: Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type clones (pCG alpha-8) was fully sequenced and found to code for neither alpha A- nor alpha D-globin. Partial sequencing of the other alpha-cDNA clones indicates that they are all of the same type.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D011817:Rabbits
chemical_list: D004274:DNA, Recombinant; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes