The interaction between methanol dehydrogenase and the autoreducible cytochromes c of the facultative methylotroph Pseudomonas AM1.
Cytochromes cH and cL were autoreduced at high pH (pK greater than 10) and the autoreduced cytochromes reacted with CO. The autoreduction was first-order with respect to oxidized cytochrome c and was reversible by lowering the pH. Pure methanol dehydrogenase reduced cytochrome c (in the absence of methanol) by lowering the pK for autoreduction to less than 8.5. A mechanism is proposed for the autoreduction of cytochrome c and its involvement in the reaction with methanol dehydrogenase.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D007700:Kinetics; D000432:Methanol; D008954:Models, Biological; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology*
D003574:Cytochrome c Group; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D000432:Methanol