Restriction and modification in Bacillus subtilis: gene coding for a BsuR-specific modification methyltransferase in the temperate bacteriophage phi 3T.
The resistance of Phi3T DNA to degradation by the restriction enzyme BsuR or its isoschizomer HaeIII is due to obligatory modification of such DNA. Biochemical and genetical experiments indicate that Phi3T codes for a methyltransferase, which methylates Phi3T DNA itself or heterologous DNA at target sites 5'-GG(*)CC.
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000235:genetics* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D008780:Methyltransferases / Q000378:metabolism*
D004279:DNA, Viral; C028464:DNA modification methylase BsuR; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases