A modified enzymatic technique for production of cell suspensions of a murine fibrosarcoma.
Collagenase, elastase, and mild mechanical procedures were used to obtain numerous viable single cells in suspension from a murine fibrosarcoma of C57BL/6 origin. This technique produced suspensions with minimal debris. The suspensions were more than 90% viable and yielded over 1.25 x 10(8) cells/g of solid tumor.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D005354:Fibrosarcoma / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D003012:Microbial Collagenase; D009374:Neoplasms, Experimental / Q000473:pathology; D010196:Pancreatic Elastase; D013048:Specimen Handling
D010196:Pancreatic Elastase; D003012:Microbial Collagenase