Do endogenous prostaglandins modulate noradrenergic transmission in the rat isolated perfused vas deferens?
The effects of the prostaglandin synthetase inhibitors aspirin, indomethacin and meclofenamic acid have been studied on the response of the rat isolated perfused vas deferens. None of these drugs, up to a concentration of 5 x 10(-5) M affected either phase of the biphasic constrictor response to 30 s periods of field stimulation. In the same preparations an inhibition of the response to field stimulation was seen in the presence of prostaglandin E1 at concentrations of 1 to 5 ng ml-1. All three prostaglandin synthetase inhibitors, at 5 x 10(-5) M, caused significant reduction of prostaglandin biosynthesis by homogenates of rat vas deferens. It is, therefore, suggested that stimuli which activate directly the noradrenergic nerves in the rat vas do not activate simultaneously a release of endogenous prostaglandins.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors; D004558:Electric Stimulation; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008469:Meclofenamic Acid / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism; D010477:Perfusion; D011458:Prostaglandins E / Q000502:physiology*; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects*; D014649:Vas Deferens / Q000187:drug effects*
D016861:Cyclooxygenase Inhibitors; D011458:Prostaglandins E; D008469:Meclofenamic Acid; D001241:Aspirin; D009638:Norepinephrine; D007213:Indomethacin