title: 31P nuclear magnetic resonance spectroscopy of lipopolysaccharides from Pseudomonas aeruginosa.
abstract: Intact lipopolysaccharide antigens isolated from seven different immunotypes of Pseudomonas aeruginosa have been examined by 31P-NMR spectroscopy. These macromolecular complexes contain phosphorus covalently attached to the carbohydrate residues present in the lipid A moiety and the 'core' oligosaccharide region. The spectral signals for various ortho- and pyrophosphoric esters were observed. All phosphate groups appeared to be monoesterified. Certain shifts characteristic for phosphate diester groups, observed in lipopolysaccharide complexes from other Gram-negative bacteria, were absent. Furthermore, no evidence was found to indicate that phosphate groups are involved in the covalent linkage of individual lipopolysaccharide complexes to form dimers or trimers.
mesh_terms: D011756:Diphosphates / Q000032:analysis; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013045:Species Specificity
chemical_list: D011756:Diphosphates; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D009943:Organophosphorus Compounds