Electron paramagnetic resonance crystallography of 17O-enriched oxycobaltomyoglobin: stereoelectronic structure of the cobalt dioxygen system.
An electron paramagnetic resonance crystallographic study was made on oxycobaltomyoglobin with the dioxygen ligand enriched to 19.1% in (17)O. There are two spectroscopically distinct cobalt dioxygen species. The less abundant species, II (40%), has nonequivalent oxygen atoms with superhyperfine tensors (O)A(alpha) = (5, -67.5, 22.4)G and (O)A(beta) = (5.4, -83.3, 30.3)G. Together with the previously reported (59)Co hyperfine tensor [Chien, J. C. W. & Dickinson, L. C. (1972) Proc. Natl. Acad. Sci. USA 69, 2783-2787], the orbital spin densities are found to be O(alpha)(p(eta)) = 0.48, O(alpha)(p(zeta)) = -0.11, O(beta)(p(eta)) = 0.74, O(beta)(p(zeta)) = -0.16, Co(d(xz)) = -0.01, Co(d(yz)) = 0.06 for a total electron density of 1.01. The O-O axis is directed toward His-E7, suggesting a possible hydrogen bonding interaction which may contribute to the nonequivalency of the oxygen atoms; its projection approximately bisects N(1)-Fe-N(2). The z axis of the (Co)A tensor is tilted at an angle of 28 degrees from the heme normal, resulting in a Co-O-O angle of 120 degrees . The more abundant species, I (60%), has equivalent oxygen atoms with (O)A(gamma) = (12, -72.5, 20)G and orbital spin densities of O(gamma)(p(eta)) = 0.54, O(gamma)(p(zeta)) = -0.05, Co(d(xz)) = -0.02, Co(d(yz)) = 0.09 for a total spin density of 1.10. Although the direction cosines for this molecule cannot be precisely determined, the projection of its O-O axis approximately bisects N(2)-Fe-N(3) and is parallel to the imidazole ring of His-F8. Increase of temperature changes g, (Co)A, and (O)A values, with the largest effect seen with (O)A. This temperature dependence indicates averaging of the two bond structures which are stabilized at 77 K.
D000818:Animals; D003035:Cobalt; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme; D009211:Myoglobin; D010103:Oxygen Isotopes; D011487:Protein Conformation; D013696:Temperature; D014907:Whales
D009211:Myoglobin; D010103:Oxygen Isotopes; D003035:Cobalt; D006418:Heme