Detection of infectious centers in C57BL/Ka lymphoid cell populations infected in vitro by the radiation leukemia virus.
The cocultivation of nonproducer lymphoma cells derived from a radiation-induced lymphoma of the C57BL/Ka mouse with cultures of lymphoid cell populations from the thymus, spleen, and marrow of the same strain 48 hr after their infection by the C57BL/Ka leukemia viruses permits the detection of infectious centers in these cultures. A quantitative assay is described which allows the estimation in lymphoid cell subpopulations of the numbers of target cells susceptible to productive infection by the thymotropic and leukemogenic viruses of C57BL/Ka mice in vitro. This assay should greatly facilitate the identification and characterization of such target cells.
D000818:Animals; D001854:Bone Marrow Cells; D002470:Cell Survival; D002478:Cells, Cultured; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D007942:Leukemia, Experimental / Q000382:microbiology*; D008214:Lymphocytes / Q000382:microbiology*; D008223:Lymphoma / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000382:microbiology*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology / Q000382:microbiology