title: DNase I hypersensitive sites in Drosophila chromatin occur at the 5' ends of regions of transcription.
abstract: By using a map of the unique region of DNA encoding the fur small heat-shock proteins of Drosophila melanogaster (hsp 22, hsp 23, hsp 26, and hsp 28), and a simple mapping technique, the positions of the DNase I hypersensitive sites of chromatin in the vicinity of these genes have now been determined. The major chromatin-specific sites occur at the 5' ends of each of the four heat-shock protein genes in embryo nuclei. These genes are not active in the nuclei analyzed but can be quickly induced in these cells by the heat-shock stimulus. The chromatin structure indicated by DNase I hypersensitivity may be a necessary factor in the general mechanism of gene activation.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D003851:Deoxyribonucleases / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D011506:Proteins; D004247:DNA; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes