Separation of modulator-dependent protein kinase (type I) from cyclic GMP-dependent protein kinase in mouse testes.
A new type of enzyme, modulator-dependent protein kinase (type I) (M-PKI), was successfully isolated from the cytosol fraction of mouse testes. It was eluted slightly after the peak of cyclic GMP-dependent protein kinase (G-PK) by Sephadex G-200 gel filtration. Unlike either cyclic AMP-dependent protein Kinase (A-PK) or G-PK, its maximal activity depended exclusively on the presence of crude protein kinase modulators (PKM) or partially purified stimulatory modulator (PKMs).
D000818:Animals; D002850:Chromatography, Gel; D006152:Cyclic GMP / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011494:Protein Kinases / Q000302:isolation & purification*; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology*
D011494:Protein Kinases; D006152:Cyclic GMP