title: Active accumulation and exchange transport of chloride in astroglial cells in culture.
abstract: Chloride transport in primary cultures of astroglial cells from rat brain shows saturation kinetics, is inhibited by SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid), and exchanges with HCO-3. These properties are similar to those of the anion exchange system in erythrocytes. Estimated of intracellular C1- concentration([C1-]i) in the primary cultures give values in the range of 31 to 43 mM, which are 3- to 5-times greater than predicted from equilibration with an average measured membrane potential of -70 mV, suggesting that these cells also actively accumulate Cl-.
mesh_terms: D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D001693:Biological Transport, Active; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D008564:Membrane Potentials; D010042:Ouabain / Q000494:pharmacology; D051381:Rats
chemical_list: D001639:Bicarbonates; D002712:Chlorides; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D010042:Ouabain; D003994:Bucladesine