title: Inhibitory effect on the formation of chlamydial inclusions in McCoy cells by seminal fluid and some of its components.
abstract: Our purpose was to determine the effect of semen and some of its components upon chlamydial inclusion (CI) count and cytopathogenic effect in cycloheximide-treated McCoy cells. Semen and spermine caused a dose-related decrease in the number of CI, using a standardized infectious dose of Chlamydia trachomatis, immunotype F. Lysozyme in certain concentrations stimulated CI formation, whereas the cations Cu++ and Zn++ produced a cytopathogenic effect and a dose-related reduction of the CI count, EDTA neutralized the effects of the cations and its addition to seminal fluid experimentally infected with C. trachomatis resulted in a greater number of CI than found in control cultures containing only semen.
mesh_terms: D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000187:drug effects*; D003300:Copper / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D002479:Inclusion Bodies / Q000187:drug effects*; D008297:Male; D009113:Muramidase / Q000494:pharmacology; D010088:Oxidoreductases / Q000494:pharmacology; D012661:Semen / Q000032:analysis / Q000502:physiology*; D012756:Sheep; D015032:Zinc / Q000494:pharmacology
chemical_list: D003300:Copper; D004492:Edetic Acid; D010088:Oxidoreductases; D009113:Muramidase; D015032:Zinc