title: Virus-mediated abrogation of chicken lymphocyte responsiveness to mitogenic stimulus.
abstract: We have found that various sorts of virus particles, including avian leukosis and sarcoma viruses, Sendai virus, Friend leukemia virus and murine mammary tumor virus, are able, upon coincubation with chicken peripheral lymphocytes and either Concanavalin A (Con A) or phytohemagglutinin (PHA), to inhibit the mitogenic responses that normally follow. Such inhibition is not dependent on the use of infectious virus, and can be documented by using particles whose infectivity has been abolished by irradiation with ultraviolet light. Lymphoid cells that had been preincubated with viruses at concentrations approximating 10 particles per cell and for only 2 hours were inhibited by as much as 83% in ability to respond to the lectins employed. These results appear to be mediated, at least in part, by a virus-induced factor with the capacity to abrogate the responsiveness of freshly obtained lymphocytes to mitogenic stimulus. This factor is devoid of interferon activity, as tested in a vesicular-stomatitis-virus plaque reduction assay.
mesh_terms: D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012328:RNA Viruses / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology
chemical_list: D010835:Phytohemagglutinins; D003208:Concanavalin A