title: A lysosomal enzyme involved in diphosphatidylglycerol degradation.
abstract: A soluble lysosomal phosphodiesterase in rat liver that hydrolyzes monoacylglycerophosphorylglycerophosphorylglycerol (AGPGPGase) was shown to be distinct from a lysosomal acid phosphodiesterase IV (PDase IV) which catalyzes the hydrolysis of bis(p-nitrophenyl) phosphate. The criteria used to distinguish lysosomal AGPGPGase from PDase IV were: separation on ion exchange celluloses, dissimilar inhibition patterns and different rates of inactivation on concentration. The lysosomal PDase IV activity was competitively inhibited by inorganic phosphate with a Ki value of 0.33 mM phosphate and was inhibited by a number of organophosphoryl compounds including AGPGPG, phosphatidylcholine, phosphatidylinositol, ATP and 4-methylumbelliferylpyrophosphate.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000818:Animals; D002308:Cardiolipins / Q000378:metabolism*; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D009596:Nitrophenols / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
chemical_list: D002308:Cardiolipins; D009596:Nitrophenols; D010726:Phosphodiesterase Inhibitors; C002887:bis(4-nitrophenyl)phosphate; D010727:Phosphoric Diester Hydrolases; C030411:monoacylglycerophosphorylglycerophosphorylglycerol phosphodiesterase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4