Selective release of plasma-membrane enzymes from rat hepatocytes by a phosphatidylinositol-specific phospholipase C.
When isolated hepatocytes are incubated with phosphatidylinositol-specific phospholipase C, three cell-surface enzymes show markedly different behaviour. Most of the alkaline phosphatase is released at very low values of phosphatidylinositol hydrolysis, whereas further phosphatidylinositol hydrolysis releases only a maximum of about one-third of the 5'-nucleotidase. Alkaline phosphodiesterase I is not released. If cells containing phosphatidyl[3H]inositol are similarly treated, then the released [3H]inositol is in the form of inositol phosphate: no evidence has been obtained for any covalent association between released [3H]inositol and alkaline phosphatase.
D015720:5'-Nucleotidase; D000469:Alkaline Phosphatase; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D009708:Nucleotidases; D010716:Phosphatidylinositols / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology*; D051381:Rats; D010738:Type C Phospholipases / Q000494:pharmacology*
D010716:Phosphatidylinositols; D010740:Phospholipases; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D010738:Type C Phospholipases