A promoter of pBR322 activated by cAMP receptor protein.
We have demonstrated in vitro the existence on the plasmid pBR322 of a promoter signal that is strictly dependent on cAMP and its receptor protein CRP. Transcription initiates with pppG at nucleotide 2270 and proceeds counterclockwise on the standard pBR322 map. DNase protection studies show that CRP selectively binds to the -35 region of the promoter. This region exhibits strong structural homologies to the binding sites of other CRP-dependent promoters.
D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology*; D002373:Cyclic AMP Receptor Protein; D004262:DNA Restriction Enzymes; D003851:Deoxyribonucleases; D009876:Operon; D010957:Plasmids / Q000187:drug effects; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*
D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes