Two-dimensional analysis of proteins sedimenting with simian virus 40 chromosomes.
The nonhistone proteins sedimenting in low-salt glycerol gradients with simian virus 40 chromosomes were analyzed by two-dimensional gel electrophoresis, utilizing nonequilibrium pH gradients as the first dimension and sodium dodecyl sulfate-gel electrophoresis as the second dimension. By densitometric quantitation of the radiolabeled proteins present in each fraction of the gradients, it was possible to identify sedimenting with all or a fraction of the simian virus 40 chromosomes. VP-1 sedimented with simian virus 40 chromosomes; additional evidence for its binding to chromosomes was obtained by immunochemical techniques. Four proteins (Mr 25,000, pI 6.0; Mr 32,000, pI 7.2; Mr 35,000, pI 8.5; and Mr 80,000, pI 7.2) sedimented with specific subsets of chromosomes.
D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D004586:Electrophoresis; D008970:Molecular Weight; D012093:Replicon; D013539:Simian virus 40 / Q000032:analysis* / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis*
D002843:Chromatin; D014764:Viral Proteins