Involvement of a high-molecular-weight polyprotein translational product of Snyder-Theilen Feline sarcoma virus in malignant transformation.
The previously described high-molecular-weight polyprotein major translational product of the Snyder-Theilen strain of feline sarcoma virus (FeSV) was shown to possess protein kinase activity with specificity for tyrosine acceptor sites. Cells transformed by Snyder-Theilen FeSV exhibited constitutively elevated levels of phosphotyrosine and a concomitant reduction in epidermal growth factor (EGF) binding sites. By endpoint cloning in microtiter plates, a number of transformation-defective (tf) mutants of the Snyder-Theilen strain of FeSV were isolated. Mink cells nonproductively infected by such mutants were morphologically nontransformed, failed to grow in soft agar, bound EGF as efficiently as control mink cells, and lacked rescuable transforming virus. Although the level of expression of the major viral polyprotein translational product in td mutant-infected clones was comparable to that of wild-type (wt) transformants, the polyprotein in mutant clones lacked detectable protein kinase activity and total cellular phosphotyrosine levels were not elevated significantly above control values. Of a large number of wt Snyder-Theilen FeSV-transformed mink cell clones isolated, the majority were found to revert to a nontransformed morphology upon continuous passage in cell culture. Such nontransformed variants, as well as a Gardner FeSV-transformed mink cell revertant, lacked detectable polyprotein expression and exhibited levels of phosphotyrosine and EGF binding similar to those of control mink cells. These findings provide strong evidence favoring the involvement of the Snyder-Theilen FeSV-encoded high-molecular-weight polyprotein and its associated tyrosine-specific protein kinase activity in transformation.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003673:Defective Viruses / Q000378:metabolism; D004622:Embryo, Mammalian; D066246:ErbB Receptors; D007668:Kidney; D008907:Mink; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000502:physiology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D012190:Retroviridae / Q000378:metabolism*; D015682:Retroviridae Proteins, Oncogenic; D005253:Sarcoma Viruses, Feline / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
D011956:Receptors, Cell Surface; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; C029554:high-molecular-weight polyprotein, feline sarcoma virus; D014443:Tyrosine; D011494:Protein Kinases; D066246:ErbB Receptors