Induction of prophage and mutagenic effects by alkyl alkylaminosulfonates, ethyl aminosulfonate and alkyl methanesulfonates.
Prophage induction and mutation by alkylaminosulfonates, ethyl aminosulfonate and alkyl methanesulfonates were examined comparatively. Prophage induction was carried out with a lysozyme lysis technique on the lysogenic strain Micrococcus lysodeikticus 53-40 (N5). The sulfonic ester derivatives show a slight lysogenic induction. At higher concentrations their toxicity seems to mask phage detection. Only methyl isopropylaminosulfonate and ethyl aminosulfonate exhibit no or negligible toxic effects, and with these compounds at higher concentrations a strong prophage induction is found. Alkyl sulfonate derivatives induce mutations in the tester strain of Salmonella typhimurium TA1535. Methyl methylaminosulfonate and ethyl N-methyl-N-2-chloroethyl aminosulfonate show a mutagenicity comparable to that of the well-known methyl methanesulfonate or ethyl methanesulfonate. With ethyl aminosulfonate, however, which does not show inactivation, no significant mutagenic effect was observed. DNA alterations were found in the polymerase-deficient strain E. coli P3478. The results of prophage induction and mutagenicity are compared and discussed.
D000476:Alkanesulfonates / Q000494:pharmacology*; D000818:Animals; D004256:DNA Polymerase I / Q000172:deficiency; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D008242:Lysogeny; D008837:Micrococcus / Q000187:drug effects* / Q000235:genetics; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014775:Virus Activation / Q000187:drug effects*
D000476:Alkanesulfonates; D009153:Mutagens; D004256:DNA Polymerase I