title: Presentation of viral antigens by human vascular endothelial cells in vitro.
abstract: Endothelial cells (EC) separated from the umbilical vein were shown to be free of contaminating monocytes. EC could replace peripheral blood-derived macrophages as antigen-presenting cells for in vivo sensitized T cells towards a variety of viral antigens. The T-cell--CE-antigen response was also specificity inhibited by anti-HLA-DR antisera. T cells primed by antigen together with autologous macrophages could be restimulated by antigen pulsed HLA-D/DR identical EC in an antigen specific secondary response, indicating a similar mechanism for antigen presentation by EC or macrophages.
mesh_terms: D000956:Antigens, Viral; D002450:Cell Communication; D002455:Cell Division; D004306:Dose-Response Relationship, Immunologic; D004727:Endothelium / Q000276:immunology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008264:Macrophages; D010544:Peroxidases / Q000494:pharmacology; D018139:Simplexvirus / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology; D014373:Tuberculin; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D000956:Antigens, Viral; D007106:Immune Sera; D014373:Tuberculin; D010544:Peroxidases