A direct, rapid, radioassay for adenosine diphosphatase.
Radiolabelled adenosine diphosphate [beta 32Pi]ADP was synthesized from radioactive orthophosphate and adenosine-5'-monophosphate with dicyclohexylcarbodiimide. The purified radioactive adenosine diphosphate was used as the substrate in a rapid, direct, one-step assay for adenosine diphosphatase in human neutrophilic leukocyte homogenates. Certain kinetic properties of the enzyme activity have been determined.
D001081:Apyrase / Q000097:blood*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D009504:Neutrophils / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000097:blood*; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D010744:Phosphoric Monoester Hydrolases; D001081:Apyrase