Measurement of 5'-nucleotidase in mouse peritoneal macrophages in listeriosis.
5'-Nucleotidase activity in peritoneal macrophages of mice was measured both before and after infection with Listeria monocytogenes in hosts which possessed high or low anti-listerial resistance either due to a genetically determined trait or as a result of splenectomy. Reduction in enzyme activity was directly related to the degree of infection that developed in the hosts and hence was inversely related to the level of anti-listerial resistance observed. Basal 5'-nucleotidase activity was significantly lower in noninfected hosts that possessed high anti-listerial resistance, possibly reflecting a higher level of native macrophage activation in these hosts.
D015720:5'-Nucleotidase; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D007111:Immunity, Cellular; D008088:Listeriosis / Q000201:enzymology* / Q000276:immunology; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D009708:Nucleotidases / Q000378:metabolism*; D013045:Species Specificity; D013154:Spleen / Q000502:physiology
D009708:Nucleotidases; D015720:5'-Nucleotidase