Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.
A serological trapping technique for detecting rotaviruses is described which involves coating electron microscope grids with protein A and specific rotavirus antiserum. The presence of a layer of antibodies on the grid increases the number of rotavirus particles that can be visualized. Thirty-five crude fecal extracts from infants suffering from diarrhea were examined by the serological trapping technique and by standard electron-microscopy. When the specimens were deposited on antibody-coated girds, 71% of them were found to contain virus particles, compared with 20% on standard uncoated grids. The method is simple and rapid and does away with the need to concentrate the specimens.
D000327:Adsorption; D003968:Diarrhea, Infantile / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D008854:Microscopy, Electron; D012087:Reoviridae / Q000302:isolation & purification*; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*; D013205:Staphylococcal Protein A / Q000276:immunology
D013205:Staphylococcal Protein A