The crystallization of mitochondrial cytochrome oxidase-cytochrome c complex.
Conditions are described under which crystals are formed with equimolar complex of mitochondrial cytochrome oxidase and cytochrome c. Characteristic absorption bands of the solubilized crystals could be attributed to the cytochrome oxidase-cytochrome c complex with heme a:c ratio of 2:1. Activity of crystals shows more close heme-heme interaction between two cytochromes than that of the mixture.
D000818:Animals; D002417:Cattle; D003460:Crystallization; D003574:Cytochrome c Group / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000302:isolation & purification*; D008722:Methods; D008929:Mitochondria, Heart / Q000201:enzymology*; D013053:Spectrophotometry
D003574:Cytochrome c Group; D003576:Electron Transport Complex IV