A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacterophage T4.
Mutations in the v gene of bacteriophage T4 are associated with a marked increase in sensitivity to killing by UV radiation at 254 nm, but not to a variety of other forms of base damage to DNA. Early studies from this laboratory provided evidence for a role of the v gene in the excision of pyrimidine dimers (PD) from DNA. Specifically, it was shown that extracts of T4v+-infected Escherichia coli catalyse the formation of single-strand breaks (nicks) and/or alkali-labile sites in UIV-irradiated duplex DNA. Comparable hydrolysis of phosphodiester bonds is not observed with extracts of E. coli infected with the mutant T4v1 (ref. 5). The product of the v gene has been extensively purified in a number of laboratories; however, convincing evidence of purification to physical homogeneity has not yet been presented.
D004247:DNA / Q000235:genetics; D004260:DNA Repair; D004279:DNA, Viral / Q000528:radiation effects; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014466:Ultraviolet Rays
D004279:DNA, Viral; D011740:Pyrimidine Dimers; D004247:DNA; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)