title: Plaque formation and purification of BK virus in cultured human urinary cells.
abstract: Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.
mesh_terms: D001739:BK Virus / Q000032:analysis / Q000254:growth & development* / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D011120:Polyomavirus / Q000254:growth & development*; D014556:Urine / Q000166:cytology* / Q000382:microbiology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral