Fluorescent-light-induced lethality and DNA repair in normal and xeroderma pigmentosum fibroblasts.
Cell survival and induction of endonuclease-sensitive sites in DNA were measured in human fibroblast cells exposed to fluorescent light or germicidal ultraviolet light. Cells from a xeroderma pigmentosum patient were hypersensitive to cell killing by fluorescent light, although less so than for germicidal ultraviolet light. Xeroderma pigmentosum cells were deficient in the removal of fluorescent light-induced endonuclease sites that are probably pyrimidine dimers, and both the xeroderma pigmentosum and normal cells removed these sites with kinetics indistinguishable from those for ultraviolet light-induced sites. A comparison of fluorescent with ultraviolet light data demonstrates that there are markedly fewer pyrimidine dimers per lethal event for fluorescent than for ultraviolet light, suggesting a major role for non-dimer damage in fluorescent light lethality.
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004260:DNA Repair / Q000528:radiation effects*; D004720:Endonucleases / Q000494:pharmacology; D005347:Fibroblasts / Q000528:radiation effects*; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D011740:Pyrimidine Dimers / Q000528:radiation effects; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000503:physiopathology*
D011740:Pyrimidine Dimers; D004720:Endonucleases