Prostacyclin-dependent activation of adenylate cyclase in a neuronal somatic cell hybrid: prostanoid structure-activity relationships.
1 Prostacyclin activates adenylate cyclase of the NCB-20 neuronal hybrid cell line. 2 There is a guanosine 5'-triphosphate requirement for the activation of adenylate cyclase by 5,6 beta-dihydroprostacyclin (a stable analogue of prostacyclin). 3 Steady-state kinetic analysis of the activation of adenylate cyclase by 5,6 beta-dihydroprostacyclin suggests a simple non-cooperative bimolecular interaction between the ligand and single receptor population. 4 Structure-activity relationships of selected prostanoids elucidated certain functional requirements for activation of adenylate cyclase.
D011452:15-Oxoprostaglandin 13-Reductase / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D011464:Epoprostenol / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006822:Hybrid Cells / Q000201:enzymology*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000201:enzymology*; D011453:Prostaglandins / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013997:Time Factors
D011453:Prostaglandins; D006160:Guanosine Triphosphate; D011464:Epoprostenol; D006911:Hydroxyprostaglandin Dehydrogenases; D011452:15-Oxoprostaglandin 13-Reductase; D000262:Adenylyl Cyclases