title: An extensive deletion causing overproduction of yeast iso-2-cytochrome c.
abstract: CYC7-H3 is a cis-dominant regulatory mutation that causes a 20-fold overproduction of yeast iso-2-cytochrome c. The CYC7-H3 mutation is an approximately 5 kb deletion with one breakpoint located in the 5' noncoding region of the CYC7 gene, approximately 200 base from the ATG initiation codon. The deletion apparently fuses a new regulatory region to the structural portion of the CYC7 locus. The CYC7-H3 deletion encompasses the RAD23 locus, which controls UV sensitivity and the ANP1 locus, which controls osmotic sensitivity. The gene cluster CYC7-RAD23-ANP1 displays striking similarity to the gene cluster CYC1-OSM1-RAD7, which controls, respectively, iso-1-cytochrome c, osmotic sensitivity and UV sensitivity. We suggest that these gene clusters are related by an ancient transpositional event.
mesh_terms: D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000031:analogs & derivatives* / Q000096:biosynthesis; D045304:Cytochromes c; D005808:Genes, Recessive; D005809:Genes, Regulator; D008040:Genetic Linkage; D009154:Mutation; D009996:Osmotic Fragility; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D014466:Ultraviolet Rays
chemical_list: D003574:Cytochrome c Group; C032139:iso-2-cytochrome C; D045304:Cytochromes c