Control of opiate receptor-adenylate cyclase interactions by calcium ions and guanosine-5'-triphosphate.
Adenylate cyclase of homogenates of NG108-15 neuroblastoma x glioma hybrid cells is activated by low concentrations of Ca2+ ions and is inhibited by higher (greater than 0.1 mM) concentrations of Ca2+ ions. Activation of either opiate receptors by 10 microM morphine or alpha-adrenergic receptors by 10 microM norepinephrine inhibits adenylate cyclase by 55% in the absence of Ca2+ ions, and inhibits the Ca2+-dependent activation of adenylate cyclase by more than 90%. Concentrations of Ca2+ ions greater than 0.1 mM inhibit adenylate cyclase and also reduce the extent inhibition of adenylate cyclase by morphine but not by norepinephrine. Guanosine-5'-triphosphate (0.1-1 microM) is required for inhibition of adenylate cyclase by morphine. The results show that morphine inhibits adenylate cyclase by a guanosine-5-triphosphate-dependent process and that the extent of inhibition of adenylate cyclase by morphine or norepinephrine is a function of the Ca2+ ion concentration and the proportion of adenylate cyclase molecules that are activated or inhibited by Ca2+ ions.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000201:enzymology; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D009020:Morphine / Q000494:pharmacology; D009447:Neuroblastoma / Q000201:enzymology; D009638:Norepinephrine / Q000494:pharmacology; D011957:Receptors, Opioid / Q000187:drug effects*
D000067956:Adenylyl Cyclase Inhibitors; D011957:Receptors, Opioid; D009020:Morphine; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases; D002118:Calcium; D009638:Norepinephrine