Mutation caused by human phagocytes.
Histidine-requiring mutants of Salmonella typhimurium TA100 were incubated with human peripheral blood leukocytes. More of these bacteria reverted to histidine independence than controls not incubated with cells. Phagocyte-rich suspensions were mutagenic, while heat-killed cells, lymphocytes, or mixed blood leukocytes of a patient with chronic granulomatous disease were not. Production of reactive oxygen metabolites could explain the capacity of phagocytes to induce mutation.
D006105:Granulomatous Disease, Chronic / Q000503:physiopathology; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D009152:Mutagenicity Tests; D009154:Mutation; D010100:Oxygen / Q000009:adverse effects*; D010545:Peroxides / Q000009:adverse effects; D010586:Phagocytes / Q000502:physiology*; D012486:Salmonella typhimurium / Q000235:genetics; D013481:Superoxides / Q000009:adverse effects
D010545:Peroxides; D013481:Superoxides; D010100:Oxygen