Characterization of JC papovavirus adapted to growth in human embryonic kidney cells.
Human papovavirus JC virus was adapted to growth in human embryonic kidney (HEK) cells. After eight passages, the HEK-adapted JC virus produced high virus yields and was capable of forming plaques in HEK monolayer cultures. Eleven plaque-purified stocks were prepared and characterized. Biologically, the plaque-purified virus induced tumor and viral antigens in HEK cells earlier and in a higher percentage of cells than uncloned virus. Cytopathic changes were also evident sooner and were more extensive. The DNA from uncloned as well as plaque-purified isolates was analyzed by restriction endonuclease cleavage followed by gel electrophoresis. The DNA from uncloned HEK-adapted virus was heterogeneous. Plaque-purified virus isolates yielded DNA which, although much less heterogeneous than the uncloned stock, still consisted of two or more species of viral DNA.
D002478:Cells, Cultured; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004622:Embryo, Mammalian; D006801:Humans; D007668:Kidney / Q000166:cytology*; D011120:Polyomavirus / Q000254:growth & development*; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
D004279:DNA, Viral; D004262:DNA Restriction Enzymes