beta-Endorphin: radioreceptor binding assay. Relative potency of synthetic analogs with various chain lengths.
An assay system is described to measure the specific binding of beta-endorphin to opiate sites (receptors) in rat brain membrane preparations using the tritiated hormone as the primary ligand. By this assay procedure, the radioreceptor activity of beta-endorphin and synthetic analogs with various chain lengths has been determined. The results suggest that both NH2- and COOH-terminal sequences of the molecule are involved in the interaction of beta-endorphin with opiate receptors.
D000818:Animals; D001923:Brain Chemistry; D002462:Cell Membrane; D004723:Endorphins / Q000032:analysis* / Q000138:chemical synthesis / Q000502:physiology; D008297:Male; D010446:Peptide Fragments / Q000032:analysis; D011869:Radioligand Assay; D051381:Rats; D011957:Receptors, Opioid / Q000032:analysis; D001615:beta-Endorphin
D004723:Endorphins; D010446:Peptide Fragments; D011957:Receptors, Opioid; D001615:beta-Endorphin