title: A comparison of composition and fluidity of multiple sclerosis and normal myelin.
abstract: Myelin was isolated from normal-appearing white matter from the brains of 11 multiple sclerosis (MS) patients, 11 normal individuals, and 1 patient with subacute sclerosing panencephalitis (SSPE). Ratios of protein, cholesterol, and phospholipid content, total fatty acid content, and total amino acid content were determined. The lipid bilayer fluidity of the myelin was measured using electron spin resonance and fluorescence polarization spectroscopy. The protein-to-phospholipid ratio was higher than normal, the cholesterol-to-protein ratio was lower than normal while the cholesterol-to-phospholipid ratio was normal in MS myelin. This suggested a relative increase of protein to lipid content in MS myelin. Of the fatty acid content of a total lipid extract, MS myelin had relatively more 16:0, less 18:3, and less 22:3, 24:1 than normal. The total amino acid content of MS myelin was altered in a way which suggested a decreased percentage of basic protein and an increased percentage of proteolipids. SSPE myelin had similar changes in amino acid content but not in protein-to-cholesterol or -phospholipid ratios or fatty acid content as MS myelin. There was no significant difference in myelin fluidity, however, between MS and normal myelin using fatty acid spin labels or fluorescent probes. A correlation was found between the fluidity and the cholesterol-to-phospholipid ratio but the MS and normal samples varied over a similar range. Although these alterations in lipid and protein composition had no effect on fluidity, they may nevertheless have serious consequences for myelin structure.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000032:analysis; D001921:Brain / Q000503:physiopathology; D001923:Brain Chemistry; D002784:Cholesterol / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008560:Membrane Fluidity; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000503:physiopathology*; D009185:Myelin Proteins / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis / Q000502:physiology*; D010743:Phospholipids / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D009185:Myelin Proteins; D010743:Phospholipids; D002784:Cholesterol