Lack of spontaneous and inducible natural killer cell activity in human bone marrow: presence of adherent suppressor cells.
Human bone marrow cells collected from ribs of patients undergoing thoracotomy had low or no natural killer (NK) cell activity against K562 in a 4-hour chromium release assay. In vitro overnight treatment with interferon or interleukin 2 of bone marrow cells resulted in no induction or augmentation of NK cell activity. In the presence of adherent bone marrow cells interferon was unable to enhance NK cell activity of blood lymphocytes, although the baseline level of NK cell activity was not suppressed. These results suggest that adherent bone marrow cells regulate the development of active NK cells and that bone marrow components do not provide a favorable environment for the functional differentiation of NK cells.
D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007376:Interleukin-2 / Q000502:physiology; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000954:Antigens, Surface; D007370:Interferon Type I; D007376:Interleukin-2; D016169:Lymphocyte Function-Associated Antigen-1