title: Structure and genomic organization of the mouse dihydrofolate reductase gene.
abstract: The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5' untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003062:Codon; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
chemical_list: D003062:Codon; D012333:RNA, Messenger; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes; D008727:Methotrexate