Changes in polypeptide pattern in ASV-transformed rat cells are correlated with the degree of morphological transformation.
Normal rat kidney cells (NRK) have been transformed with an avian sarcoma virus (ASV) mutant that induces a temperature-sensitive transformed phenotype. When compared in vitro different ASV-transformed clones showed different degrees of morphological transformation at the permissive temperature, while they all reverted to normal at 39 degrees C. Comparisons of the polypeptide patterns of the different cells showed two things. First, a group of about 30 polypeptides, amounting to 5% of all the polypeptides analyzed in normal cells, were affected in all ASV-transformed clones and also in two papovavirus-transformed clones and also in two papovavirus-transformed NRK clones. Second, the patterns were found to differ between the clones in that varying numbers of polypeptides were found to be affected depending on the clone; the number of polypeptide changes showed a good correlation with the degree of morphological transformation. At the nonpermissive temperature the polypeptide patterns of ts ASV-transformed cells were indistinguishable from that of the untransformed cells, which is in agreement with the morphological data.
D000818:Animals; D001358:Avian Sarcoma Viruses; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D007526:Isoelectric Point; D007668:Kidney; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D027383:Papillomaviridae; D027382:Polyomaviridae; D051381:Rats
D008565:Membrane Proteins; D009363:Neoplasm Proteins