Freezing and thawing of myosin with no alteration in ATPase activity.
Myosin can be frozen in liquid nitrogen (-70 degrees C) and stored at this temperature for 5 months with no loss in K+, Ca2+, or actin + Mg2+ -stimulated ATPase activities. Furthermore, myosin can be refrozen and thawed in this manner for at least 5 consecutive times with no alteration in ATPase activity.
D000199:Actins / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004355:Drug Stability; D005615:Freezing; D008274:Magnesium / Q000494:pharmacology; D009218:Myosins / Q000378:metabolism*; D011817:Rabbits; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors
D000199:Actins; D000251:Adenosine Triphosphatases; D009218:Myosins; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium