title: A second site specific endonuclease from Thermus thermophilus 111,Tth111II.
abstract: A second site specific endonuclease with a novel specificity has been isolated from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80 degrees C and requires Mg2+ or Mn2+ for activity. Tth111II cleaves phi X174RFDNA into 11 fragments. From the analysis of 5' terminal sequences of the phi X174RFDNA fragments produced by Tth111II action, it was concluded that Tth111II recognized the DNA sequence (See formula in text) and cleaved the sites as indicated by arrows.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D013379:Substrate Specificity; D013824:Thermus / Q000201:enzymology*
chemical_list: D008345:Manganese; D004262:DNA Restriction Enzymes; C027716:endodeoxyribonuclease Tth111II; D015252:Deoxyribonucleases, Type II Site-Specific; D008274:Magnesium