Minimal molecular requirements for reactivity of tumor cells with T cells.
We have investigated the minimal molecular requirements for T cell recognition of a previously described myeloma tumor (MOPC-315-EL), which reversibly alters its reactivity with T cells. Enucleation of MOPC-315 cells, either sensitive or resistant to reaction with T cells, did not alter the ability of the resulting cytoplasts to elicit or serve as targets for anti-H-2 or anti-Sendai viral cytotoxic T lymphocytes (CTL), despite the fact that there were no detectable differences in serologically defined H-2 or viral antigens on their surface. Likewise, when plasma membranes isolated from sensitive or resistant cells were tested for their ability to elicit anti-H-2 CTL, they retained the phenotype of the cells from which they were isolated. However, solubilized and partially purified H-2 antigens from both sensitive and resistant cells were able to elicit H-2-restricted anti-Sendai virus CTL when incorporated into liposomes with purified viral glycoproteins, and anti-H-2 CTL when incorporated alone into liposomes. These results suggest that a cellular surface component(s) exists that is probably not an H-2K or H-2D gene product and is responsible for the reversible variation in the reactivity of the tumor cells with T cells.
D000818:Animals; D000914:Antibodies, Viral; D002467:Cell Nucleus; D003593:Cytoplasm / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D008081:Liposomes / Q000494:pharmacology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000914:Antibodies, Viral; D006183:H-2 Antigens; D007106:Immune Sera; D008081:Liposomes