DNA translocation by the restriction enzyme from E. coli K.
The restriction endonuclease Eco K binds to a host specificity site and then proceeds to cleave the DNA at sites that may to several thousand bases away. It does this by translocating the DNA past the enzyme in an ATP-dependent reaction that results in the formation of highly twisted loop intermediates. DNA cleavage can occur on either side of the host specificity site.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D010957:Plasmids; D011485:Protein Binding; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004270:DNA, Circular; D000255:Adenosine Triphosphate; D004262:DNA Restriction Enzymes