Cellular interactions in the human T-T autologous mixed lymphocyte reaction.
Human peripheral blood T cells and T cell subsets were studied for the proliferative response in the autologous mixed lymphocyte reaction (AMLR) upon stimulation with mitogen- or AMLR-activated T cells and T cell subsets. T cells exhibited a vigorous proliferative response when stimulated with autologous activated T cells but not with unactivated T cells. The stimulatory capacity of activated T cells in the T-T AMLR was inhibited by prior treatment with 7.2 anti-DR monoclonal antibody in the absence of complement A BUdR and light experiment clearly demonstrated that T cells responding to activated T cells in the AMLR were distinct from those responding in allogeneic MLR. Further analysis with OKT4+ and OKT8+ T cell subsets demonstrated that OKT8+ T cells were the major responder upon stimulation with PHA-activated T cells. In contrast, OKT4+ T cells were the major responder in the T-T AMLR, when AMLR-activated T-cells were used as stimulators. T cells activated in T-T AMLR (PHA-activated T cells as stimulators) demonstrated suppressor activity against the proliferative response of autologous responder T cells. This study demonstrates that distinct subsets of T cells respond in the T-T AMLR and express suppressor activity.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D007959:Lymphocyte Culture Test, Mixed; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D007519:Isoantigens; D010835:Phytohemagglutinins