title: Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.
abstract: The chemical synthesis of four new deoxyribooligonucleotides to be used as adaptors in molecular cloning of DNA for expression studies is described. These are (i) start-adaptor, (ii) stop-adaptor, (iii) conversion adaptor to insert ribosomal binding site, and (iv) retrieving adaptor. We have also synthesized a 19-bases-long primer, 5'-TTGTAAAACGACGGCCAGT-3' to increase the speed of DNA sequence analysis and also demonstrated its application using single-stranded M13 DNa vector.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D006801:Humans; D007328:Insulin / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*
chemical_list: D004274:DNA, Recombinant; D007328:Insulin; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes