Secretion of alpha 1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids.
The human hepatoma cell line SK-HEP-1 has been shown by radioimmunoelectrophoresis to synthesize and secrete a protein which coprecipitates with human alpha 1-antitrypsin. This protein was indistinguishable from serum alpha 1-antitrypsin in terms of electrophoretic mobility, apparent subunit molecular weight (47,000), and binding to concanavalin-A. The protein identified as alpha 1-antitrypsin (alpha 1 AT) was secreted by seven clones derived from SK-HEP-1 and by twelve out of eighteen hybrid clones derived from the fusion of SK-HEP-1 with mouse RAG cells. There was no correlation between the expression of alpha 1 AT and that of human enzymes assigned to sixteen different autosomes. There was an imperfect correlation between the expression of alpha 1 AT and of the two chromosome 9 marker enzymes AK1 and AK3 (two discordant clones).
D000230:Adenocarcinoma; D000818:Animals; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002999:Clone Cells; D003208:Concanavalin A / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008113:Liver Neoplasms; D051379:Mice; D008970:Molecular Weight; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*
D000515:alpha 1-Antitrypsin; D003208:Concanavalin A