Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
The DNA sequence of a 1.45 kb EcoRI fragment from the yeast (Saccharomyces cerevisiae) TRP1 region has been determined. The fragment contains the TRP1 gene and a yeast chromosomal replicator. The TRP1 gene has been located on the fragment by analysis of potential initiation and termination codons in the DNA sequence. This location has been confirmed by subcloning portions of the fragment. Both the 5' and 3' noncoding regions of the TRP1 gene contain sequence homologies with analogous areas surrounding other yeast genes. The yeast replicator has been localized in a region near the 3' end of the TRP1 gene. The DNA sequence in this region contains several structural features which may be involved in the initiation of DNA replication.
D019747:Aldose-Ketose Isomerases; D001483:Base Sequence; D002238:Carbohydrate Epimerases; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D007535:Isomerases; D009876:Operon; D010957:Plasmids; D012093:Replicon; D012267:Ribosemonophosphates; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic; D014364:Tryptophan / Q000235:genetics*; D062367:ortho-Aminobenzoates
D012267:Ribosemonophosphates; D062367:ortho-Aminobenzoates; D014364:Tryptophan; D004262:DNA Restriction Enzymes; D007535:Isomerases; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C029865:phosphoribosylanthranilate isomerase