[Role of microviscosity of the lipid phase of microsomal membranes in regulation of enzymatic activity of glucose-6-phosphatase under microsomal modifications in vivo and in vitro].
The changes in microviscosity of the lipid phase of microsomal membranes under microsomal modification in vivo and in vitro were studied. It was shown that in intact microsome lipids there occur five thermo-induced structural transitions within the temperature range of 5--50 degrees. Delipidation of microsomes results in a shift in structural transitions temperature. Based on the literary and own data it was assumed that the breaks on the Arrhenius plots for glucose-6-phosphatase (EC 3.1.3.9) activity are due to phase-structural changes of microsomal lipids.
D000818:Animals; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000502:physiology*; D008563:Membrane Lipids / Q000502:physiology*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity
D008563:Membrane Lipids; D005952:Glucose-6-Phosphatase
Rol' nikroviazkosti lipidnoĭ fazy mikrosomal'nykh membran v reguliatsii fermentativnoĭ aktivnosti gliukozo-6-fosfatazy pri modifikatsii mikrosom in vivo i in vitro.