Effects of antigastrin drugs on the interaction of 125I-human gastrin with rat gastric mucosa membranes.
A method for studying the interaction of 125I-human gastrin with a rat gastric mucosal membrane fraction is described, and the saturability, high affinity and reversibility of the preparations, as well as the correlation of pharmacological responses to antigastrin-drugs with the observed binding are discussed. Proglumide and other antigastrin drugs inhibit gastrin binding in a dose-dependent way, and their activities in such a system are well correlated with the "in vivo" antisecretory activities of these drugs.
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001549:Benzamides / Q000494:pharmacology; D001667:Binding, Competitive / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D006801:Humans; D011377:Proglumide / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects; D013853:Thioacetamide / Q000031:analogs & derivatives / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D001549:Benzamides; D005755:Gastrins; D011725:Pyridines; D011956:Receptors, Cell Surface; D013853:Thioacetamide; C004326:antigastrin; D011377:Proglumide; C013841:benzotript