Analysis of transcription during type C viral induction using cell permeabilization techniques.
The reversible cell permeabilization procedure of Castellot et al. has been applied to chemical induction of endogenous type C virus from mouse cells. This procedure has provided a direct demonstration of the alpha-amanitin sensitivity of viral transcription in intact cells at concentrations known to inhibit RNA polymerase II.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D008722:Methods; D051379:Mice; D010539:Permeability; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors; D012190:Retroviridae / Q000254:growth & development*; D014158:Transcription, Genetic; D014775:Virus Activation / Q000187:drug effects
D000546:Amanitins; D012319:RNA Polymerase II