[Properties of phosphodiesterase from Vipera lebetina venom immobilized on Agarose].
Preparations of phosphodiesterase from Vipera lebetina venom immobilized on agarose were obtained. The kinetic properties for the hydrolyses of various substrates of soluble and immobilized phosphodiesterase, e. g. effects of pH, temperature, substrate concentrations, etc., were compared. The values of Km, V and activation energy for the substrate hydrolyses were determined.
D000818:Animals; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012685:Sepharose; D013816:Thermodynamics; D014757:Viper Venoms / Q000378:metabolism*
D004800:Enzymes, Immobilized; D014757:Viper Venoms; D012685:Sepharose; D010727:Phosphoric Diester Hydrolases
Izuchenie svoĭstv fosfodiesterazy iada giurzy, immobilizovannoĭ na agaroze.