RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.
A new type II sequence-specific endonuclease, RsaI, has been identified from Rhodopseudomonas sphaeroides strain 28/5. An RsaI purification scheme that yields enzyme which is free of contaminating exonuclease and phosphatase activities after a single column fractionation has been developed. The enzyme recognized the tetranucleotide sequence 5'-GTAC-3' and cleaved between the T and A, thereby generating flush ends. RsaI should be extremely useful in deoxyribonucleic acid sequencing experiments.
D001483:Base Sequence; D003090:Coliphages; D004269:DNA, Bacterial / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D010957:Plasmids; D012242:Rhodobacter sphaeroides / Q000201:enzymology*; D013539:Simian virus 40
D004269:DNA, Bacterial; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004720:Endonucleases