Cloning of the replication gene O of E. coli bacteriophage lambda and its expression under the control of the lac promoter.
The expression of the replication gene O of bacteriophage lambda was put under the control of the lac promoter-operator region integrated into the pBR322 cloning vehicle. The new plasmid pKK104 was introduced into minicells and the O gene induced by isopropyl-beta-thiogalactoside (IPTG). The O protein could be identified as a major component in extracts from these cells, in association with the cell membrane fractions. The molecular weight of the O protein in SDS gels is about 33 000, and it is metabolically unstable but apparently stable upon isolation as a membrane-associated fraction.
D010582:Bacteriophage lambda / Q000235:genetics*; D002462:Cell Membrane / Q000032:analysis; D003001:Cloning, Molecular; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
C027812:O protein, Bacteriophage lambda; D014764:Viral Proteins