[Establishment of persistent cytomegalovirus infection in primary cultured trophoblastic cells (author's transl)].
The primary cultured cells (Ch) derived from chorionic villi were infected with human cytomegalovirus (CMV). The main results obtained are as follows. 1) The primary cultured trophoblastic cell (Ch) infected with CMV have been maintained in the state of CMV persistent infection for over 6 months. These cells (Ch/CMV) were the CMV-carrier cells in a balance between the cytolysis by CMV-specific cytopathic effect (CPE) and the growth of uninfected cells, showing the CMV persistent infection at the cell population levels. 2) The Ch/CMV cells have persistently released infectious CMV with titers ranging from 1.6 X 10(2) to 1.2 X 10(4) PFU/ml into the culture fluid. CMV-specific antigens were detectable by immunofluorescent antibody staining of the virus releasing cells. 3) These Ch/CMV cells still maintained an ability to secret estrone and estradiol. When these abilities lowered in the successive cultures, the cells encountered the cytolysis by CMV release. 4) Infectious titer of CMV released from Ch/CMV cells reduced by the addition of estrogen in the culture media. In this case, however CMV-specific CPE occurred with a similar pattern as the control one lacked an addition of estrogen. 5) It was suggested that estrogen synthesis in the cells may play an important role in an establishment or a maintenance of CMV persistent infection.
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development* / Q000472:pathogenicity; D004967:Estrogens / Q000502:physiology; D005260:Female; D006801:Humans; D014327:Trophoblasts / Q000382:microbiology*; D014774:Virulence
D004967:Estrogens