Generation of pyrophosphate from extracellular ATP at the surface of HeLa cells.
On incubation of intact HeLa cells with micromolar concentrations of [gamma 32 P]ATP under physiological conditions, the generation of inorganic pyrophophate in the supernatant has been observed. The reaction depends on incubation time and cell number employed; the enzymatic activity is not shed into the supernatant. Isotope dilution data support that the responsible enzyme(s) is located at the cell surface.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D010761:Phosphorus Radioisotopes; D011494:Protein Kinases / Q000378:metabolism
D011756:Diphosphates; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D000251:Adenosine Triphosphatases