Scanning electron microscopy of frozen-cracked, dry-cracked and enzyme-digested tissue of human malignant choroidal melanomas.
By applying cracking and enzyme-digestion methods to tissue of human malignant choroidal melanomas, SEM was used to visualize the interior of the cells, their membranes, nuclei and organelles. Single cells were depicted to their full extent. Apart from a three-dimensional impression of the structures and their surfaces, the methods also made it much easier to measure cells and cell components than by conventional methods.
D002830:Choroid Neoplasms / Q000648:ultrastructure*; D005615:Freezing; D006801:Humans; D006821:Hyaluronoglucosaminidase; D006868:Hydrolysis; D008545:Melanoma / Q000648:ultrastructure*; D003012:Microbial Collagenase; D008855:Microscopy, Electron, Scanning / Q000379:methods
D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase