Syntheses of some fluorescent ribonucleotides.
Synthesis of guanosine 3'-phosphodiesters of 1, N6-ethenoadenosine-2-sulfonate or 1, N6-etheno-2-azaadenosine can be done readily with ribonuclease N1. There is a 10-fold difference in quantum yield between the phosphodiesters and the starting fluorescent nucleosides. This difference provides the basis for the development of a fluorescent assay for nuclease.
D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D002852:Chromatography, Ion Exchange; D005453:Fluorescence; D006150:Guanine Nucleotides / Q000096:biosynthesis*; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000096:biosynthesis*; D006868:Hydrolysis; D006163:Ribonuclease T1 / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D013057:Spectrum Analysis
D006150:Guanine Nucleotides; C027905:1,N(6)-etheno-2-azaadenosine guanosine 3'-phosphodiester; C027904:1,N(6)-ethenoadenosine-2-sulfonate guanosine 3'-phosphodiester; D006157:Guanosine Monophosphate; D012260:Ribonucleases; D006163:Ribonuclease T1; D000241:Adenosine