[Chromatin structure with no nucleosomes: dissociated SV40 virus].
The mild dissociation of SV40 virus particles at pH 7.5 yields a nucleoprotein complex containing all the capsid proteins and histones of the intact virion. Electron microscopic observations show a bead-on-a-string structure comprising about 70 particles 7 nm in diameter along the viral DNA. No particle of nucleosome size was visible. The compaction of DNA in this complex is lower than 2 and is likely to be close to unity.
D000818:Animals; D002213:Capsid / Q000032:analysis; D002460:Cell Line; D002521:Cercopithecus; D002875:Chromosomes / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D007668:Kidney; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000032:analysis; D009707:Nucleosomes / Q000648:ultrastructure; D013539:Simian virus 40 / Q000648:ultrastructure*; D014764:Viral Proteins / Q000032:analysis
D006657:Histones; D009698:Nucleoproteins; D009707:Nucleosomes; D014764:Viral Proteins
Une structure chromatinienne dépourvue de nucléosomes: le virion dissocié de SV40.