[Quaternary structure of NAD-kinase from rabbit liver].
A procedure for isolation of NAD-kinase from rabbit liver resulting in 4000-fold purification and the activity yield of 50-60% is described. The molecular weight of the NAD-kinase subunit determined by SDS electrophoresis is 30 000. The purified enzyme is a dimer. Partially purified preparations of NAD kinase contain multiple forms with mol. Weights ranging from 650 000 to 180 000 and have complex kinetic behaviour. A thermostable activator of NAD-kinase which, when added to the homogeneous enzyme preparation, destroys the linear dependence of the enzyme specific activity on concentration, was detected. The nature of multiple forms of NAD-kinase and the possible role of the activator in their formation is discussed.
D000818:Animals; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011487:Protein Conformation; D011817:Rabbits
D046911:Macromolecular Substances; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase
Chetvertichnaia struktura NAD-kinazy iz pecheni krolika.