Affinity purification of newly phosphorylated protein molecules. Thiophosphorylation and recovery of histones H1, H2B, and H3 and the high mobility group protein HMG-1 using adenosine 5'-O-(3-thiotriphosphate) and cyclic AMP-dependent protein kinase.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D013873:Thionucleotides / Q000494:pharmacology*; D013950:Thymus Gland
D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases