The differences in purine metabolism between T and B lymphocytes.
In this study enzyme activities involved in purine metabolism were measured in T and B lymphocytes separated by E and EAC rosetting methods. Adenosine deaminase, purine nucleoside phosphorylase and HGPRTase activities were significantly elevated in T cells, compared to the activities in B cells. There were no significant differences in adenosine kinase and APRTase activities between T and B lymphocytes. In contrast, PRPPsynthetase activities were higher in B cells than in T cells. The uptake of various radiolabeled precursors by mitogen stimulated lymphocytes was studied. The uptake of 14C-formate by the mitogen stimulated lymphocytes was markedly lower, compared to that of 14C-adenosine and or 14C-purine bases. The uptake of 14C-adenosine by PHA stimulated lymphocytes was considerably higher than that of Con A or PWM stimulated lymphocytes. However, the uptake of 14C-hypoxanthine into lymphocytes stimulated with PWM was increased by comparison with unstimulated lymphocytes. From these results it seems that adenosine plays a central role in the metabolism of T cells, and that purine bases are preferentially utilized in B cells.
D000243:Adenosine Deaminase / Q000378:metabolism; D001402:B-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007042:Hypoxanthines / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012268:Ribose-Phosphate Pyrophosphokinase / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D007042:Hypoxanthines; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D011687:Purines; D003208:Concanavalin A; D011683:Purine-Nucleoside Phosphorylase; D012268:Ribose-Phosphate Pyrophosphokinase; D000243:Adenosine Deaminase; D013936:Thymidine