Mass spectographic analysis of stimulated release of endogenous amino acids from rat hippocampal slices.
Amino acids released from in vitro rat hippocampal slices following stimulation of the Schaffer's collateral pathway, were collected by micro-perfusion and analysed by chemical ionization mass spectrometry, with isotope ratiodetermination as the quantitative technique, through the use of stable, isotopically labeled internal standards. Stimulation at 10 pulses/sec resulted in a doubling of the release of aspartic acid over the resting level and about a 60% increase in glutamate release, which is in essential agreement with studies utilizing K+ depolarization-evoked release from slices.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D046508:Culture Techniques; D004292:Dominance, Cerebral / Q000502:physiology; D005971:Glutamates / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D013058:Mass Spectrometry; D009434:Neural Pathways / Q000378:metabolism; D051381:Rats; D009435:Synaptic Transmission
D000596:Amino Acids; D005971:Glutamates; D001224:Aspartic Acid