Immunological reaction between sera from neutron-irradiated W/FU rats and mouse mammary tumor virus.
Sera from W/Fu rats from the presence of anti mouse mammary tumor virus (MMTV) antibodies were examined by the use of an immunofluorescence (IF) test and an immune adherence hemagglutination (IAHA) test. Sera from W/Fu rats subjected to neutron irradiation or neutron irradiation followed by grafting of pituitary tumor (MtT) as a source of prolactin gave a positive IF reaction with mouse mammary tumor cells (MMT) producing type-A and type-B virus particles. Nine out of 20 (45.0%) sera from rats given irradiation alone, 17 out of 25 (68.0%) sera from rats subjected to irradiation followed by grafting of MtT and 8 out of 10 (80%) sera from rats given only grafting of MtT gave a positive reaction with the cells, whereas 5 out of 19 (26.3%) sera from normal rats showed a positive reaction with the cells. The specificity of the reaction was confirmed by the distribution pattern of specific fluorescence in MMT cells and by absorption experiments with suitable materials. The results of the IAHA test also showed that some sera from W/Fu rats reacted specifically with purified MMTV, although the incidence of appearance of antibodies in rat sera as detected by IAhA test was low as compared with that detected by IF tests. The IAHA titers of positive rat sera ranged from 1:8 to 1:128. Furthermore, the above results suggest that MtT grafting into W/Fu rats, whether or not they are subjected to neutron irradiation, results in an increase in the frequency of rats carrying antibodies against MMTV.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001769:Blood / Q000528:radiation effects*; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice; D008892:Milk / Q000276:immunology; D009502:Neutrons; D051381:Rats; D011919:Rats, Inbred Strains
D000914:Antibodies, Viral