Isolation of a gene from Drosophila by complementation in yeast.
Transformation of mutant yeast cells by cloned genomic DNA from a higher eukaryote has made it possible to isolate a Drosophila DNA sequence that complements a yeast adenine-8-mutation. A 0.8-kilobase poly(A)-containing RNA is transcribed from the cloned Drosophila segment in transformed yeast cells and can account for functional expression of the gene.
D000225:Adenine; D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004262:DNA Restriction Enzymes; D000225:Adenine