Induction of angiotensin I-converting enzyme rat lung with Captopril (SQ 14225).
Angiotensin I-converting enzyme (ACE, EC 2.4.15.1.) was measured in serum and in pulmonary plasma membranes of 40 spontaneously hypertensive rats (SHR, Okamoto Aoki strain), divided into 4 groups, and treated with SQ 14225 (Captopril), 0.2 mg . ml-1 in drinking water, for 0-24 weeks. Serum ACE activity increased 2.5-3 fold after 12-24 weeks of SQ 14225 treatment, paralleled by an increase of ACE concentration in purified pulmonary plasma membranes (25-52%), and in ACE concentration upon solubilization with Triton X-100 from such plasma membranes (96-120%). We conclude that the ACE inhibitor, SQ 14225, causes marked induction of pulmonary ACE biosynthesis. High serum ACE activity probably reflects increased total biosynthesis of the enzyme.
D000818:Animals; D002216:Captopril / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000096:biosynthesis* / Q000097:blood; D011392:Proline / Q000031:analogs & derivatives*; D051381:Rats
D011392:Proline; D002216:Captopril; D007703:Peptidyl-Dipeptidase A