The polypeptide composition of isolated surface projections of avian infectious bronchitis virus.
Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 M-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 M-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86,000 and 66,000 mol. wt. The larger polypeptide was glycosylated.
D003332:Coronaviridae / Q000032:analysis*; D001351:Infectious bronchitis virus / Q000032:analysis* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D010455:Peptides; D014764:Viral Proteins