Action of streptothricin F on ribosomal functions.
The effect of streptothricin F on elongation factor-dependent and on elongation factor-free translation systems was studied. Streptothricin F inhibits factor-dependent as well as factor-free polypeptide synthesis. The results suggest that streptothricin F inhibits polypeptide synthesis via interaction with the ribosome. In partial reactions streptothricin F impairs EF-G-dependent translocation and to a lesser extent EF-Tu-dependent binding of aa-RNA to the ribosome, while it does not affect peptide bond formation significantly.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D010649:Phenylalanine / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis; D012270:Ribosomes / Q000187:drug effects*; D013309:Streptothricins / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; D010649:Phenylalanine; D013309:Streptothricins