Cellular uptake of retinoic acid in vitro.
Incubation of chick embryo skin and mouse colon tumour 26 with [3H]retinoic acid resulted in the formation of a complex of retinoic acid and its cellular binding protein both in cytosol and in nuclei. Formation of the ligand--protein complex was temperature-dependent and increased with increases in retinoic acid concentration in the incubation medium. About 3--8% of the ligand present in the cytosol was associated with the nuclei.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D003110:Colonic Neoplasms / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D051379:Mice; D018168:Receptors, Retinoic Acid; D012867:Skin / Q000166:cytology / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism*
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin