Identification of the dnaA and dnaN gene products of Escherichia coli.
A specialized transducing lambda phage carrying the dnaN genes of Escherichia coli specifies two proteins of about 41 and 48 kilodaltons (kd). The temperature-sensitive mutations, dnaN59 and dnaA167, were found to result in altered isoelectric points of the 41 and 48 kd proteins, respectively. Thus the dnaN gene product was identified as a weakly acidic 41 and 48 kd protein. The synthesis of the dnaN gene product is greatly reduced by insertion of a transposon Tn3 in the dnaA gene and by deletion in the gene at the distal end to the dnaN gene. Temperature-sensitive dnaA mutations, on the dnaN gene product. These results indicate that the synthesis of the dnaN gene product is dependent on the structural integrity of the dnaA gene.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D004261:DNA Replication; D004251:DNA Transposable Elements; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009154:Mutation; D013696:Temperature; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D004251:DNA Transposable Elements