title: Cyclic AMP receptor proteins interacts with lactose operator DNA.
abstract: The wildtype lac promoter-operator region of E. coli was reinvestigated for cyclic AMP receptor protein (CRP) binding sites using the 'footprinting' method. Two distinct interaction sites were found, one in the -60 base pair region, which has previously been described, and a second one covering the lac operator. These two sites are similar in extent, orientation and the characteristic pattern of CRP-induced DNaseI enhanced cutting sites.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007763:Lac Operon; D010957:Plasmids; D011485:Protein Binding; D011953:Receptors, Cyclic AMP / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D011953:Receptors, Cyclic AMP; D004262:DNA Restriction Enzymes