Respiration in rat cerebral astrocytes from primary culture.
Respiration was measured polarographically in astrocytes from dissociated neonatal rat cerebra grown in primary culture. Cells grown in a modified Eagle's minimal essential medium containing 20% fetal calf serum for 14 to 21 days respired at a rate of 34.23 n at. equiv 0 mg protein-1 min-1. Cells which were grown in this medium for 14 days and the grown in serum-free medium containing 0.25 mM dibutyrylcyclic-AMP (dbcAMP) for three to six days, had respiratory rates 20% higher than in cells from untreated control cultures (P = 0.005). Maximal inhibition by oligomycin required 200 pmole per mg protein for dbcAMP-treated cells and 80 pmole  per mg protein for untreated cells. Oligomycin inhibited the respiration of both dbcAMP-treated and untreated cells by about 70%. At a concentration of 175 nmole per mg protein, 2,4-dinitrophenol (DNP) produced a maximal stimulation of respiration, which was 191% of the resting rate in dbcAMP-treated cells and 218% of the resting rate in untreated cells. The maximal DNP-stimulated respiratory rates were the same in dbcAMP-treated and untreated cells.
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004140:Dinitrophenols / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats
D004140:Dinitrophenols; D009840:Oligomycins; D003994:Bucladesine