Neuronal (Na+,K+)-ATPase and the release of purines from mouse and rat cerebral cortex.
Slices of mouse or rat cerebral cortex were incubated with [3H]adenine or [3H]adenosine, and [14C]GABA. Purines and GABA could subsequently be released by ouabain. The release of purines previously shown to occur on restoring elevated K+ levels to normal was not mimicked by noradrenaline at concentrations which activate (Na+,K+)-ATPase. Potassium-free solutions evoked no release of purine during the test period, but resulted in a large release when K+ was restored to normal. K+-free solutions evoked an immediate release of GABA. It is concluded that (Na+,K+)-ATPase is not involved in purine release.
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011687:Purines / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D011687:Purines; D005680:gamma-Aminobutyric Acid; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium