Sialic acids in bone demineralised in organic solvents.
The sialic acid content of bovine shin bone, demineralised by a new process in ethanol-trimetrylammonium EDTA, was measured and compared with that of the original whole bone. New variants of the direct Ehrlich procedure, as well as the periodate-thiobarbituric acid assay, were employed. About 75% of the sialic acid remains in the bone matrix from ethanolic demineralisation, and the PAS reaction is correspondingly much more intense, compared with matrix from aqueous EDTA demineralisation.
D000818:Animals; D001857:Bone Matrix / Q000032:analysis*; D002417:Cattle; D003648:Decalcification Technique; D004492:Edetic Acid; D000431:Ethanol; D010504:Periodic Acid; D010503:Periodic Acid-Schiff Reaction; D012794:Sialic Acids / Q000032:analysis*; D013858:Thiobarbiturates
D012794:Sialic Acids; D013858:Thiobarbiturates; D010504:Periodic Acid; D000431:Ethanol; D004492:Edetic Acid