Effects of detergents, proteins, and lipids on 2':3'-cyclic-nucleotide 3'-phosphodiesterase activity.
The myelin marker 2':3'-cyclic-nucleotide 34'-phosphodiesterase (CNPase) was isolated to a lipid- and phosphate-free stage. The effects of exogenously added lipids were tested on this preparation and compared to the known stimulation of the enzyme by detergents and proteins. CNPase could be stimulated 2-3 fold by these various agents which appeared to be additive in their effect. Enzyme-protein and enzyme-lipid interactions and possible medical use of the improved assay conditions for CNPase employed in the study are discussed.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003902:Detergents / Q000494:pharmacology*; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000201:enzymology*; D010743:Phospholipids / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D008055:Lipids; D010743:Phospholipids; D011506:Proteins; D013501:Surface-Active Agents; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases