Development of an anticomplement immunoenzyme test for detection of EB virus nuclear antigen (EBNA) and antibody to EBNA.
An anticomplement immunoenzyme test was developed by conjugating anti-human C3 antibody with horseradish peroxidase. EBNA could be detected by this test in all cell lines related to EB virus, as well as in nasopharyngeal carcinoma cells, but not in unrelated cell lines. Antibody to EBNA could also be detected. The test is sensitive and does not require a fluorescence microscope which makes it particularly suitable for mass survey.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002277:Carcinoma / Q000276:immunology; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D009303:Nasopharyngeal Neoplasms / Q000276:immunology; D011817:Rabbits
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003165:Complement System Proteins