Effects of pesticides on activities of enzymes and microorganisms in a clay soil.
Laboratory experiments were conducted to determine the effect of 32 pesticides applied at 2 levels on populations of microorganisms, activities of urease, dehydrogenase, phosphatase and nitrogenase in a clay loam incubated for 1 week. Results indicated that a decrease in bacterial number was observed with thiram for 2 days and stimulation with chlorpyrifos after 7 days. Some fungicides and fumigants inhibited fungal numbers for 2 days. The recovery was rapid and stimulatory effects on microbial numbers were evident in many samples. None of the pesticides inhibited soil urease drastically. Formazan formation was not suppressed vigorously by the treatments. With the exception of DD and Vorlex at a high level, none of the treatments inhibited phosphatase in the hydrolysis of p-nitrophenyl disodium orthophosphate. A temporary decrease in nitrogenase activity in acetylene (C2H2) reduction was observed with many pesticides. The low amount of pesticides applied to the clay loam is unlikely to have detrimental effects on soil microbes and the enzymes important to soil fertility.
D000114:Acetylene / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects*; D005658:Fungi / Q000187:drug effects*; D010088:Oxidoreductases / Q000032:analysis; D010575:Pesticides / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D014510:Urease / Q000032:analysis
D010575:Pesticides; D012987:Soil; D010088:Oxidoreductases; D010744:Phosphoric Monoester Hydrolases; D014510:Urease; D000114:Acetylene