Glycoproteins and sialyl transferase of human B lymphoblastoid cell lines.
We used two radiolabeling methods to study glycoproteins on the surface of lymphoblastoid cells. One of the methods affects tritiation of residues which are oxidized with galactose oxidase and the other causes tritiation of neuraminic acid residues. This approach was shown to allow a better resolution of cell surface glycoproteins than if either method were used alone. Glycoproteins of B1--19 cells which harbor the Epstein-Barr virus genomes were compared with those of its parental cell line, BJAB, which does not harbor the viral genomes. These studies did not reveal a unique viral protein. A 28,000 mol. wt. glycoprotein was found to be the most prominent neuraminic acid-labeled product of B1--19 cells and also of the two other cell lines, Raji and Ly38, which harbor the EBV genomes. A similar molecular weight species from BJAB cells identified by galactose oxidase labeling might be deficient in neuraminic acid residues as it was poorly labeled by the periodate oxidation method. The neuraminic acid content and level of sialyl transferase of BJAB cells were found to be lower than those of the other cell lines studied.
D001402:B-Lymphocytes / Q000032:analysis*; D002051:Burkitt Lymphoma; D002460:Cell Line; D005692:Galactose Oxidase / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D009438:Neuraminic Acids / Q000032:analysis; D009439:Neuraminidase / Q000378:metabolism; D010504:Periodic Acid / Q000378:metabolism; D012799:Sialyltransferases / Q000032:analysis*; D014166:Transferases / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
D006023:Glycoproteins; D009363:Neoplasm Proteins; D009438:Neuraminic Acids; D014764:Viral Proteins; D010504:Periodic Acid; D005692:Galactose Oxidase; D014166:Transferases; D012799:Sialyltransferases; D009439:Neuraminidase