title: Cyclic adenosine 3',5'-monophosphate-independent phosphorylation of externally oriented plasma membrane proteins in transformed and nontransformed mouse embryo cell lines.
abstract: Thirty transformed and nontransformed mouse embryo cell lines were analyzed to determine if increased plasma membrane phosphorylation is a characteristic of all transformed cell lines. Nontransformed, virally and chemically transformed, and smooth surface-transformed cell lines derived from Swiss, BALB/c, AKR, or C3H mice were studied. Cyclic AMP-independent phosphorylation of plasma membrane proteins was comparable in nontransformed and simian virus 40 (SV40)-transformed Balb/3T3 cells, in nontransformed and 3-methylcholanthrene (MCA)-transformed C3H/10T 1/2 cells, and in nontransformed and MCA-transformed AKR-2B cells. In contrast, increased plasma membrane phosphorylation was observed in MCA- and smoooth surface-transformed Balb/3T3 cells and in SV40-transformed Swiss 3T3 cells compared to the respective nontransformed parent cell lines. These observations suggest that increased cyclic AMP-independent phosphorylation of externally oriented plasma membrane proteins is not a universal marker for the transformed phenotype.
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D008564:Membrane Potentials; D051379:Mice; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D000242:Cyclic AMP; D011494:Protein Kinases