[Effects of some protein kinases, cyclic nucleotides and specific inhibitors of phosphorylation on the mitotic cycle of Physarum polycephalum].
The true slime mould Physarum polycephalum was treated with various agents by spraying them upon the cell surface 4 hrs before the second synchronous mitosis. The onset of mitosis was considerably approximated after the plasmodium treatment with protein kinases from rat hepatoma or Ph. polycephalum at the late G2 phase. The catalytic and regulatory subunits of cAMP-dependent pig brain protein kinase caused retardation of mitosis, while the holoenzyme, casein kinase and alkaline phosphatase did not affect the timing of mitosis. The cyclic nucleotides and inhibitors of their metabolic enzymes were used to investigate the role of phosphorylation processes in the mitotic cycle.
D000818:Animals; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D008938:Mitosis / Q000187:drug effects*; D010804:Physarum / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP
Vliianie nekotorykh proteinkinaz, tsiklicheskikh nukleotidov i spetsificheskikh ingibitorov reaktsiĭ fosforilirovaniia na vremia nastupleniia mitoza u miksomitseta Physarum polycephalum.