Subcellular distribution and some properties of N-ethylmaleimide-sensitive and-insensitive forms of glycerol phosphate acyltransferase in rat adipocytes.
1. Glycerol phosphate acyltransferase (GPAT) activities were measured in subcellular fractions obtained from rat epididymal adipocytes. These contained both N-ethylmaleimide-sensitive and N-ethylmaleimide-insensitive forms of the enzyme. 2. As shown by parallel measurements of marker enzymes, N-ethylmaleimide-insensitive GPAT is most probably a mitochondrial activity, whereas N-ethylmaleimide-sensitive GPAT is the microsomal enzyme. 3. Subcellular distributions are also reported for dihydroxyacetone phosphate acyltransferase (DHAPAT) (assayed with and without N-ethylmaleimide), monoacylglycerol phosphate acyltransferase (MGPAT) and Mg2+-dependent and Mg2+-independent forms of phosphatidate phosphohydrolase (PPH).
D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D000217:Acyltransferases / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009713:Nucleotidyltransferases / Q000378:metabolism; D010711:Phosphatidate Phosphatase / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D003567:Cytidine Diphosphate Diglycerides; D005994:Glycerophosphates; D004099:Dihydroxyacetone Phosphate; D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; C020051:glycerone-phosphate O-acyltransferase; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D009713:Nucleotidyltransferases; C022348:phosphatidate cytidylyltransferase; D010711:Phosphatidate Phosphatase; D005033:Ethylmaleimide