Preparation of two dinor-PGI2 metabolites from 6-keto-PGF1 alpha by Mycobacterium rhodochrous.
The transformation of 6-keto-PGF1 alpha to two prostacyctin metabolites, 2,3-dinor-6-keto-PGF1 alpha (I) and 2,3-dinor-6,15-diketo-13,14-dihydro-PGF1 alpha (II) by Mycobacterium rhodochrous UC-6176 is described. The finding that the bacterium oxidized 6-keto-PGF1 alpha to the 6,15-diketo metabolite II shows that it contains 15-hydroxy prostaglandin dehydrogenase and delta 13 reductase enzyme systems.
D011452:15-Oxoprostaglandin 13-Reductase / Q000378:metabolism; D015121:6-Ketoprostaglandin F1 alpha / Q000031:analogs & derivatives; D000818:Animals; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D009161:Mycobacterium / Q000378:metabolism*; D010084:Oxidation-Reduction; D011460:Prostaglandins F / Q000378:metabolism*; D011461:Prostaglandins F, Synthetic / Q000378:metabolism*
D011460:Prostaglandins F; D011461:Prostaglandins F, Synthetic; D015121:6-Ketoprostaglandin F1 alpha; C028290:2,3-dinor-6-ketoprostaglandin F1alpha; C028291:2,3-dinor-6,15-diketo-13,14-dihydroprostaglandin F1alpha; D006911:Hydroxyprostaglandin Dehydrogenases; D011452:15-Oxoprostaglandin 13-Reductase