Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.
An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be explained solely by the lack of exonuclease III activity, because other xth mutants tested, including a deletion mutant, repaired radiation-induced strand breaks at close to the normal rate.
D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism* / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D009154:Mutation; D014965:X-Rays
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D005092:Exonucleases