Immunofluorescent detection of double-stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus.
Urea treatment of ethanol-fixed virus-infected cells exposed nucleic acid antigens for immunofluorescence. Three double-stranded (ds) RNA-containing viruses showed bright fluorescence using antibodies against dsRNA. Three single-stranded RNA-containing viruses showed less intense fluorescence with anti-dsRNA. Four out of five cell lines persistently infected with various RNA-containing viruses showed no dsRNA detectable by immunofluorescence.
D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007243:Infectious bursal disease virus / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000276:immunology; D012367:RNA, Viral / Q000032:analysis* / Q000276:immunology; D012087:Reoviridae / Q000032:analysis* / Q000254:growth & development / Q000276:immunology
D012330:RNA, Double-Stranded; D012367:RNA, Viral