An enzyme-linked immunosorbent assay for detection of antibodies to avian adenovirus and avian adenovirus-associated virus in chickens.
An enzyme-linked immunosorbent assay system (ELISA) was adapted for the detection of antibodies to avian adenovirus (AV) and avian adenovirus-associated virus (A-AV). Both before and after exposure, sera from chickens undergoing natural and experimental infections were assayed by ELISA, virus neutralization (VN), and immunodiffusion (ID) for antibody to both CELO virus and A-AV. The ELISA system was found to be comparable to VN for determining antibody concentrations to CELO virus and A-AV. In many cases, ELISA was found to be more sensitive than ID.
D000256:Adenoviridae / Q000276:immunology*; D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000259:Aviadenovirus / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D000229:Dependovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000276:immunology*
D000914:Antibodies, Viral