Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli.
The denV gene of bacteriophage T4 has been cloned into Escherichia coli K-12 by inserting appropriate fragments of cytosine-containing T4 DNA into the Sal I site of the plasmid pBR322. The denV gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in DNA by UV. In uvrA recA mutants, deficient in an early step in excision repair, the cloned DNA results in enhanced UV resistance that is more pronounced in stationary- than in exponential-phase cultures. The expression of the cloned DNA also results in the enhanced survival of UV-irradiated phage lambda or of a denV mutant of phage T4 and in removal of dimers from the DNA of UV-irradiated cells.
D003001:Cloning, Molecular; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011740:Pyrimidine Dimers / Q000235:genetics; D013604:T-Phages / Q000235:genetics* / Q000528:radiation effects; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D004274:DNA, Recombinant; D011740:Pyrimidine Dimers; D004262:DNA Restriction Enzymes