[Complex nature of the inhibitory effect of the cytosol on transcription in isolated nuclei of rat liver].
It was shown that rat liver cytosol contains a factor inhibiting [3H]UTP incorporation into RNA of isolated nuclei. Fractionation of cytosol by Sephadex G-100 chromatography, step-wise increase in ammonium sulfate concentration and electrophoresis in polyacrylamide gel revealed a complex nature of cytosol inhibitory activity. It was suggested that this complex nature can be due to the presence of either non-specific inhibitors of transcription (i.e. phosphatase, RNAase) or a set of virtually specific inhibitors of transcription in the cytosol.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000502:physiology; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic
D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases
O slozhnoĭ prirode ingibiruiushchego deistviĭa tsitozola na transkriptsiiu v izolirovannykh iadrakh pecheni krys.