[Effect of hydrocortisone on alpha-glycerophosphate dehydrogenase activity in KOX-1 and mouse glioblastoma EPNT-5 cells].
A comparative cytochemical investigation for alpha-glycerophosphate dehydrogenase (alpha-GPhD) activity in transplanted cellular line of mouse glioblastoma (EPNT-5) and KOX-1 was performed under normal conditions and under hydrocortisone effect. In glioblastoma EPNT-5 cells a high alpha-GPhD activity was revealed. The enzymatic activity was found to correspond to the dynamics of cell multiplication. Under the effect of hydrocortisone, alpha-GPhD activity demonstrated a two-fold increase, while alpha-GPhD activity in KOX-1 cells was very low, and hydrocortisone did not induce its increase. The data obtained supported the view on the glial nature of the transplanted ćpHT-5 line and demonstrated that the glial elements preserved their specific peculiarities at a prolonged cultivation.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002460:Cell Line; D004790:Enzyme Induction; D005910:Glioma / Q000139:chemically induced; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D006854:Hydrocortisone / Q000494:pharmacology*; D051379:Mice; D008938:Mitosis; D009053:Sarcoma Viruses, Murine; D012513:Sarcoma, Experimental
D015127:9,10-Dimethyl-1,2-benzanthracene; D005993:Glycerolphosphate Dehydrogenase; D006854:Hydrocortisone
Vliianie gidrokortizona na aktivnost' alpha-glitserofosfatdegidrogenazy v kletkakh KOX-1 i glioblastomy myshi EPNT-5.