Spermatozoal hapten gained via autolysis.
Using autolysis different spermatozoa surface peptides were solubilized. The resulting low molecular weight compounds were further separated by gel-chromatography on Biogel P-4, thin-layer chromatography on Cel 400 and thin-layer-electrophoresis. After the conjugation of the compound with cytochrome C a conformation-independent determinant was detected with immuno-electrophoresis. Out of the 17 fractions tested only fraction 3, with a molecular weight of 1800 daltons revealed a precipitation reaction with human sperm-immobilizing sera and rabbit antihuman sperm-serum. All 17 fractions showed no precipitation reaction against human sperm-agglutinating sera.
D001329:Autolysis; D002462:Cell Membrane / Q000276:immunology*; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003574:Cytochrome c Group; D004586:Electrophoresis; D006241:Haptens / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D013078:Sperm Immobilizing Agents / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
D003574:Cytochrome c Group; D006241:Haptens; D013078:Sperm Immobilizing Agents