A link between transport and plasma membrane redox system(s) in carrot cells.
Carrot (Daucus carota L.) cells grown in suspension culture oxidized exogeneous NADH. The NADH oxidation was able to stimulate K+ (86Rb+) transport into cells, but it did not affect sucrose transport. N,N'-Dicyclohexyl-carbodiimide, diethylstilbestrol, and oligomycin, which only partially inhibited NADH oxidation, almost completely collapsed the K+ (86Rb+) transport. Vanadate, which is less effective as an ion transport inhibitor, was less effective in inhibiting the NADH-driven transport of K+ (86Rb+). p-Fluormethoxycarbonylcyanide phenylhydrazone inhibits the K+ transport over 90% including that induced by NADH. The results are interpreted as evidence that a plasma membrane redox system in root cells is closely associated with the ATPase which can drive K+ transport. Because of the inhibitor effects, it appears that membrane components common to the redox system and ATPase function in the transport of K+.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D009243:NAD / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D010944:Plants / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D009243:NAD; D009249:NADP; D013395:Sucrose; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D000251:Adenosine Triphosphatases; D011188:Potassium