title: Purification and characterization of a murine basement membrane collagen-degrading enzyme secreted by metastatic tumor cells.
abstract: A type IV collagen-degrading enzyme activity secreted by a highly metastatic mouse tumor was purified by concanavalin A- and type IV collagen-agarose affinity chromatographies followed by gel filtration on Bio-Gel A-0.5 m. The apparent molecular weight of the enzyme was 160,000 but about 70,000 when Triton X-100 was added to the column buffer. The purified enzyme protein was resolved on sodium dodecyl sulfate-polyacrylamide gel electrophoresis into two polypeptide chains of about 68,000 and 62,000 daltons. The enzyme activity could be increased by preincubation with trypsin and it is possible that the two chains represent latent and active enzyme forms. The enzyme activity was not reduced in the presence of dithiothreitol, it had a pH optimum of 7.6 and was inhibited by EDTA but not N-ethylmaleimide, phenylmethylsulfonyl fluoride, or Trasylol. The inhibition with EDTA was reversible. The pro-alpha 1(IV) and pro-alpha 2(IV) chains of the type IV procollagen substrate were both degraded at a similar rate to form two pairs of degradation fragments corresponding in molecular weights to about 70 and 30% of the original size chains. The presence of Triton X-100 increased slightly the activity of the enzyme and diminished the reduction of its activity upon freezing, indicating that the enzyme is a hydrophobic protein.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000201:enzymology*; D002460:Cell Line; D002846:Chromatography, Affinity; D051379:Mice; D008810:Mice, Inbred C57BL; D003012:Microbial Collagenase / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D012513:Sarcoma, Experimental / Q000201:enzymology*
chemical_list: D003012:Microbial Collagenase