title: Sequence of the intercistronic region between the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit and coupling factor beta subunit gene.
abstract: The nucleotide sequence of the region between the genes for the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase and the beta subunit of coupling factor from tobacco chloroplast DNA has been determined. The 5' ends of their mRNAs have been located on the DNA sequence using s1 nuclease mapping. The promoter sequences of these chloroplast genes have features in common with those of prokaryotes. E. coli RNA polymerase can recognize these sequences and start transcription at authentic initiation sites.
mesh_terms: D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D002736:Chloroplasts / Q000201:enzymology; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004720:Endonucleases; D004926:Escherichia coli / Q000201:enzymology; D005796:Genes; D046911:Macromolecular Substances; D010944:Plants / Q000201:enzymology*; D010947:Plants, Toxic; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014026:Nicotiana / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase