Diagnosis of infectious bronchitis (IB) by examination of tracheal mucus for IB-precipitating antigens.
The use of tracheal exudates for detecting precipitating infectious bronchitis (IB) antigen by the agar-gel precipitin (AGP) technique is described. Of 47 experimentally infected tracheas, 24 gave positive precipitin lines between the second and 12th day postinfection; 9 out of 18 tracheas from birds from natural outbreaks of IB were also positive. Tests can be read within 18-24 hr. For best results, several antisera or an antiserum at various concentrations should be used.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001991:Bronchitis / Q000175:diagnosis / Q000662:veterinary*; D002645:Chickens; D003332:Coronaviridae / Q000276:immunology*; D003333:Coronaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D001351:Infectious bronchitis virus / Q000276:immunology*; D008297:Male; D009093:Mucus / Q000276:immunology*; D011201:Poultry Diseases / Q000175:diagnosis*; D011233:Precipitin Tests / Q000662:veterinary; D014132:Trachea
D000956:Antigens, Viral