A microassay for quantitatively detecting the Epstein-Barr virus receptor on single cells utilizing flow cytometry.
A quantitative microassay for detecting and analyzing the Epstein-Barr virus receptor (EBVR) utilizing fluorescein-conjugated virions is presented. The test is virus substrain-specific. Both the B95-8 and P3HR-1 strains were labelled and adsorbed to a variety of targets. Relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. Cell size and cellular DNA content were measured simultaneously with virus binding, thus enabling us to calculate EBVR density and to correlate receptor synthesis and cell cycle stage.
D002460:Cell Line; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D007399:Interphase; D008722:Methods; D008938:Mitosis; D011991:Receptors, Virus / Q000032:analysis* / Q000378:metabolism
D011991:Receptors, Virus