Virus-induced thymidine kinases as markers for typing herpes simplex viruses and for drug sensitivity assays.
A rapid, reproducible and objective new method for typing herpes simplex viruses type 1 (HSV-1) and type 2 (HSV-2) based on the effects of virus-induced thymidine kinases on various antiviral drugs has been developed. When several laboratory strains and clinical isolates were typed by this method and compared to the results obtained by the immunofluorescence antibody typing method, agreement was found for all the viruses. The new technique has the added advantage of determining the sensitivity of HSV strains to antiviral drugs.
D001973:Bromodeoxyuridine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D003851:Deoxyribonucleases / Q000378:metabolism; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000494:pharmacology; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007065:Idoxuridine / Q000031:analogs & derivatives / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D018139:Simplexvirus / Q000145:classification*; D013937:Thymidine Kinase / Q000378:metabolism*
C035991:5-iodouridine; C024068:5-propyl-2'-deoxyuridine; C020235:brivudine; D013937:Thymidine Kinase; D003851:Deoxyribonucleases; D001973:Bromodeoxyuridine; D007065:Idoxuridine; D003857:Deoxyuridine