[Characteristics of the pKMR plasmids found in Shigella flexneri].
The clinical isolate of Sh. flexneri 1b, resistant to 5 antibiotics and sulfonamides, has been studied by the method of conjugation and found to have a group of transfer-suppressed pKMR-plasmids: pKMR 204-1 (Ap Sm Tc Cm Km Su), pKMR 204-2 (Sm Km Su), pKMR 204-5 (Km Su) and pKMR 204-7 (Sm Tc Cm Km Su), whose molecular weight was 99, 71.2, 73.8 and 59.5 Md respectively. The treatment of the plasmids with restriction endonuclease BamHI has revealed that plasmids pKMR 204-2 and pKMR 204-5 are definitely related to plasmid KMR 204-1, being its deletion mutants. At the same time plasmids pKMR 204-1 and pKMR 204-7 differ in their sensitivity to endonuclease BamHI and stably coexist within the same cell, thus seeming to belong to different compatibility groups.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003227:Conjugation, Genetic / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D008970:Molecular Weight; D010957:Plasmids / Q000187:drug effects; D011815:R Factors / Q000187:drug effects; D012763:Shigella flexneri / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Kharakteristika pKMR-plazmid, vyiavlennykh u Shigella flexneri.