Biological activity of proviral DNA isolated from cells infected with MAV-2(0) virus.
The ability of DNA isolated from avian myeloblastosis-associated virus MAV-2(0)-infected fibroblasts to induce the synthesis of virus upon transfection of susceptible but not resistant cells was demonstrated. The virus obtained, when inoculated into 12-day-old embryos, led to the manifestation of osteopetrosis after prolonged incubation and to the induction of nephroblastomas in some cases. Positive transfection with DNA from the non-virus producing myeloblast cell line was not detected on bone marrow cells and fibroblasts preinfected with tdB77-C virus. After transfection with DNA from virus-producing myeloblasts, the reproduction of non-transforming virus was observed. This virus did not induce myeloblastosis upon infection of chickens but induced nephroblastomas on rare occasions.
D000818:Animals; D001354:Avian Leukosis Virus / Q000254:growth & development*; D009189:Avian Myeloblastosis Virus / Q000254:growth & development* / Q000472:pathogenicity; D001853:Bone Marrow / Q000382:microbiology; D002642:Chick Embryo; D002645:Chickens; D004279:DNA, Viral / Q000378:metabolism*; D005347:Fibroblasts / Q000382:microbiology; D010022:Osteopetrosis / Q000209:etiology; D014775:Virus Activation; D014779:Virus Replication
D004279:DNA, Viral