N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide(W-7), a calmodulin antagonist, also inhibits phospholipid-sensitive calcium-dependent protein kinase.
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide(W-7), commonly regarded as a calmodulin antagonist, inhibited phospholipid-sensitive Ca2+ -dependent protein kinase and to a lesser extent cyclic GMP- and cyclic AMP-dependent protein kinases. Kinetic studies of the inhibition of the homogeneous spleen phospholipid-sensitive Ca2+ -dependent protein kinase indicated that W-7 inhibited the enzyme activity competitively with respect to phospholipid (Ki = 60 microM). N-(6-Aminohexyl)-1-naphthalenesulfonamide (W-5) was found to be much less potent than W-7. The findings indicate that W-7 was able to inhibit a variety of protein kinases, in addition to those requiring calmodulin previously reported.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002417:Cattle; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology; D010718:Phosphatidylserines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology; D013449:Sulfonamides / Q000494:pharmacology*; D013552:Swine
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D010718:Phosphatidylserines; D010743:Phospholipids; D013449:Sulfonamides; C017967:W 7; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP; D002118:Calcium