In vitro enzymatic recognition of DNA modified by O,O'-diacetyl or O-acetyl derivatives of the carcinogen 4-hydroxyaminoquinoline-1-oxide.
Purified DNA was modified in vitro by 3H-labelled O-acetyl or O,O'-diacetyl-4-hydroxyaminoquinoline-1-oxide (Ac4HAQO or di Ac-4HAQO). It was then subjected to the action of the single-stranded DNA specific nuclease S1 and the digested fractions were analysed. For both types of modified DNA, the release of non-modified nucleotides was faster than the release of modified nucleotides. This result is at variance with that obtained with acetoxy-acetylaminofluorene-modified DNA: in the latter case, the modified nucleotides were preferentially released. The results suggest that the S1 endonuclease can recognize different conformational changes in DNA, which depend on the carcinogen used. The enzymatic activity (or activities) present in Micrococcus luteus cell extracts released ethanol-soluble products from Ac-4HAQO modified DNA.
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000494:pharmacology; D000634:Aminoquinolines / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D004247:DNA; D004720:Endonucleases / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D008837:Micrococcus / Q000201:enzymology
D000634:Aminoquinolines; D002273:Carcinogens; C027915:O,O-diacetyl-4-hydroxyaminoquinoline 1-oxide; D015108:4-Hydroxyaminoquinoline-1-oxide; C032982:4-acetoxyaminoquinoline 1-oxide; D004247:DNA; D004720:Endonucleases