title: Periodate-induced transformation of human peripheral blood lymphocytes and the resultant oxidation of membrane sialyl, galactosyl and fucosyl residues.
abstract: Oxidation of human peripheral mononuclear cells with sodium periodate results in lymphocyte activation. Period-date, at optimal mitogenic concentrations, oxidizes membrane sialyl residues (NeuNAc) essentially into the 7 carbon analogue (C7-NeuNAc). Fucosyl and galactosyl residues are also oxidized by periodate, since propane 1,2-diol and glycerol are isolated in acid hydrolysates of lymphocytes oxidized by periodate and reduced by tritiated borohydride. The neuraminidase pretreatment of lymphocytes induces a 40-50% decrease of their response to periodate. Neuraminidase treatment of 108 human peripheral lymphocytes liberated 9.6 microgram NeuNAc (31 nmol), representing 68.5% of the total content. The neuraminidase treatment dramatically enhances the recovery of glycerol in hydrolysates of lymphocytes treated successively with periodate and tritiated borohydride.
mesh_terms: D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects; D008566:Membranes / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology*; D012794:Sialic Acids / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
chemical_list: D006027:Glycosides; D012794:Sialic Acids; D010504:Periodic Acid; D005643:Fucose; C009288:metaperiodate; D009439:Neuraminidase; D013936:Thymidine; D005690:Galactose