title: [Combined method (suspension-monolayer) of preparing an inactivated foot-and-mouth disease vaccine].
abstract: Semiproductional and productional experiments were carried out to cultivate BHK21C13 cells in 30- and 250-litre fermentors, to produce F.M.D. virus in suspension and in roller, and to produce a F.M.D. vaccine in 500-litre automatic mixing containers, employing a combined method 'suspension-monolayer'. The F.M.D. virus that was replicated in suspension had a 10(-6,0-6,5) titer, and the virus that was obtained in monolayer - a 10(-7,0-8,0) titer. It was demonstrated that the F.M.D. vaccines that were produced after the combined method were sterile, innocuous, and immunogenic.
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D007668:Kidney / Q000166:cytology; D013535:Suspensions; D014613:Vaccines, Attenuated / Q000302:isolation & purification; D014765:Viral Vaccines / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods
chemical_list: D013535:Suspensions; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
vernacular_title: Izgotviane na inaktivirana protivoshapna vaksina po kombiniran metod (suspenziia-monosloĭ).