Enhancement of benzo(a)pyrene-induced sister chromatid exchanges as a consequence of glutathione depletion in vivo.
Pretreatment of Chinese hamsters with phorone (diisopropylidene acetone) decreased hepatic glutathione (GSH) content to about 20% of the control level after 2 hours. The GSH S-transferase activities were not affected. As a consequence of reduced detoxication by GSH the potency of 3,4-benzo(a)pyrene to induce sister chromatid exchanges in vivo in bone marrow cells was significantly enhanced. Chemically induced distinct alterations in metabolism of mutagens/carcinogens are proposed as experimental models for pharmacogenetic and toxigenetic studies.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism / Q000633:toxicity*; D006224:Cricetinae; D003434:Crossing Over, Genetic / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D008658:Inactivation, Metabolic; D007659:Ketones / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008647:Mesocricetus; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001580:Benzopyrenes; D007659:Ketones; D001564:Benzo(a)pyrene; C018637:phorone; D005978:Glutathione