In vivo identification of the transforming gene product of simian sarcoma virus.
Simian sarcoma virus (SSV) deletion mutants were constructed from a molecular clone containing the entire infectious provirus. Transfection analysis of these mutants localized the SSV transforming gene to a small region of the viral genome encompassing its cell-derived sequence (v-sis). Antiserum to a peptide synthesized on the basis of the predicted amino acid sequence of the SSV transforming gene detected a 28,000-dalton protein that was specifically expressed in SSV transformed cells and that corresponded in size to that predicted from the v-sis coding sequence. The v-sis gene product designated p28sis was not a phosphoprotein, nor did it possess detectable protein kinase activity. These findings distinguish p28sis from a number of other retroviral onc proteins.
D000818:Animals; D000914:Antibodies, Viral; D001483:Base Sequence; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D051379:Mice; D008970:Molecular Weight; D009857:Oncogenes; D010750:Phosphoproteins / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D010750:Phosphoproteins; D014764:Viral Proteins; D011494:Protein Kinases