title: The expression and functional involvement of nuclease- specific idiotype on nuclease-primed helper T cells.
abstract: The expression and functional significance of idiotypic determinants on antigen-specific helper T (Th) cell populations for responses to Staphylococcal nuclease (Nase) were evaluated in an in vitro antibody response system. Trinitrophenyl (TNP)- specific plaque-forming cell responses to TNP-conjugates of Nase (TNP-Nase) were shown to require the cooperation of Nase-primed Th cells as well as unprimed B and accessory cells. The expression on these antigen-primed Th cells of idiotypic determinants cross-reactive with those on anti-Nase antibodies was demonstrated by the specific elimination of Th cells for TNP-Nase by treatment with affinity-purified anti-idiotypic antibodies plus complement. The susceptibility of Nase-primed Th cells to elimination by such treatment was specific in that anti-idiotypic antibodies affected Th cells only from strains normally expressing the same (or a cross-reactive) idiotype on anti-Nase antibodies. A functional role of the idiotypes expressed on Nase-primed Th cells was suggested by the fact that anti-idiotypic antibody present throughout the period of culture, in the absence of complement, suppressed responses to TNP-Nase in an antigen- and strain-specific manner. It was further shown, by cell mixing experiments, that this inhibition appeared to occur at the level of the Th cells and was not dependent on the strain of origin of the B cells. Thus, antigen-specific Nase-primed Th cells express strain-specific idiotypic determinants cross-reactive with, or identical to, those of anti-Nase antibodies. These cell surface idiotypic determinants appear to be functionally involved in the activity of Th cells for the induction of antibody responses to TNP-Nase in vitro.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008211:Lymphocyte Cooperation; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009708:Nucleotidases / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013211:Staphylococcus aureus / Q000201:enzymology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007130:Immunoglobulin Idiotypes; D011947:Receptors, Antigen, B-Cell; D009708:Nucleotidases