The control of glucose 1,6-bisphosphatase by Ca2+ and calmodulin.
1. The control of glucose 1,6-bisphosphatase activity, the enzyme that degrades glucose 1,6-bisphosphate, a metabolite that regulates hexose phosphate metabolism, has been examined in a rat muscle extract. 2. The enzyme has been found to be activated by physiological Ca2+ concentrations. Ca2+ can be replaced by Sr2+ and Mn2+, but the effects are not so pronounced. 3. The Ca2+ effect is inhibited by EGTA. Trifluoperazine also inhibits enzyme activity. This effect can be reversed by adding exogenous calmodulin.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology*; D002413:Cations, Divalent / Q000494:pharmacology; D066298:In Vitro Techniques; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D002413:Cations, Divalent; C014818:glucose 1,6-diphosphate phosphatase; D010744:Phosphoric Monoester Hydrolases; D002118:Calcium