Influence of DL-ethionine on the NAD content and on the activity of the poly(ADPR)synthetase in the rat liver.
1. Ethionine, an analogue of methionine, is known to cause a number of serious changes. In this study, its influence on the NAD + NADH2 content and on the activity of the poly(ADPR)synthetase in rat liver has been investigated. 2. DL-Ethionine decreases the content of NAD + NADH2 in adrenalectomized, but not in normal animals. 3. After injection of nicotinamide the NAD + NADH2 content in the liver of adrenalectomized rats is much higher than in that of normal ones. 4. DL-Ethionine diminishes the NAD synthesis caused by nicotinamide in adrenalectomized animals and increases it in normal ones. DL-Tryptophan induces only a slight NAD synthesis, which is reduced in adrenalectomized animals by thionine. 5. 1-Methylnicotinamide slightly decreases the NAD concentration in normal animals, but increases it considerably in adrenalectomized ones. DL-ethionine reduces the NAD + NADH content in adrenalectomized animals in the presence of 1-methylnicotinamide. 6. The activity of poly(ADPR)synthetase of in-vitro tested nuclei is slightly increased after treatment with DL-ethionine.
D000315:Adrenalectomy; D000818:Animals; D005001:Ethionine / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000378:metabolism*; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D009243:NAD; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase; D005001:Ethionine