[Effect of cyclic adenosine-3',5'-monophosphate, chloramphenicol and actinomycin D on Gluconobacter oxydans biosynthesis of extracellular levansaccharase].
The biosynthesis of levansucrase by Gluconobacter oxydans was shown to be induced in media containing sorbitol or fructose. An addition of glucose at a concentration of 0.1% to the culture growing in a medium with sorbitol stimulated the biosynthesis of levansucrase, whereas glucose at a concentration of 0.5-0.6% inhibited the enzyme synthesis by 20-30%. Gluconic acid, a product of glucose metabolism, also repressed the synthesis of levansucrase, but to a lesser degree than glucose. Cyclic adenosine-3',5-'monophosphate eliminated the repression by glucose added to a medium with sorbitol. An addition of chloramphenicol to the culture growing in a medium with sorbitol showed that the induction of levansucrase required de novo protein synthesis. The inhibiting action of chloramphenicol increased with its concentration. An increase in the concentration of actinomycin D from 5 to 200 micrograms/ml inhibited the bacterial growth by 50%, but stimulated the biosynthesis of levansucrase by 40%.
D002701:Chloramphenicol / Q000494:pharmacology*; D003470:Culture Media / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006602:Hexosyltransferases / Q000096:biosynthesis*; D011548:Pseudomonadaceae / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors
D003470:Culture Media; D003609:Dactinomycin; D002701:Chloramphenicol; D000242:Cyclic AMP; D006602:Hexosyltransferases; C021513:levansucrase; D005947:Glucose
Vliianie tsiklicheskogo adenozin-3',5'-monofosfata, khloramfenikola i aktinomitsina D na biosintez vnekletochnoĭ levansakharazy Gluconobacter oxydans.