Localization of viral structural proteins in the cytoplasm and nucleus of Rous-associated virus-2-infected chicken embryo fibroblasts.
The cellular location of viral structural proteins was carried out by immunohistochemistry and by cell fractionation. Antibody against the structural protein p27 was used in immunohistochemical reactions to demonstrate the presence of viral proteins in the cytoplasm and nucleus of Rous-associated virus 2-infected chicken cells. Localization in the nucleus was found over heterochromatic regions; in the cytoplasm it was found in discrete particulate structures. These observations were extended in cell fractionation studies in which cytoplasmic and nuclear fractions were immunoprecipitated with antibody against the viral structural proteins.
D000818:Animals; D001354:Avian Leukosis Virus / Q000032:analysis* / Q000254:growth & development; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo; D003593:Cytoplasm / Q000032:analysis*; D005347:Fibroblasts; D006570:Heterochromatin / Q000032:analysis; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D014764:Viral Proteins / Q000032:analysis*
D006570:Heterochromatin; D014764:Viral Proteins