Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine.
A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus.
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D007111:Immunity, Cellular; D014176:Protein Biosynthesis; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014158:Transcription, Genetic; D014612:Vaccines; D014764:Viral Proteins / Q000235:genetics / Q000627:therapeutic use*
D004274:DNA, Recombinant; D014612:Vaccines; D014764:Viral Proteins; D004262:DNA Restriction Enzymes