Effects of cyclic AMP on S-100 protein level in C-6 glioma cells.
Dibutyryl cyclic AMP (dbcAMP) markedly elevated S-100 protein level in C-6 glioma cells in vitro. Quantitative analysis by two-dimensional polyacrylamide gel electrophoresis revealed that the elevation was caused by a combination of increased synthesis and reduced degradation of S-100 protein in C-6 cells exposed to dbcAMP. These results suggest that dbcAMP affects both the synthesis and the degradation of S-100 protein in C-6 cells.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002453:Cell Cycle; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000378:metabolism*; D007700:Kinetics; D009374:Neoplasms, Experimental / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D009418:S100 Proteins / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D003994:Bucladesine; D000242:Cyclic AMP