Elevated NAD(P) glycohydrolase activity: a possible enzymatic marker for malignancy in Burkitt's lymphoma cells.
A comparative analysis of enzymatic activities has been performed on 47 human continuous lymphoid lines: 22 tumors derived from Burkitt's lymphoma lines, 6 other lymphomatous long-term cultures, and 19 nonmalignant ties determined on the cell extracts. 4 showed no significant differences between the various lines. They included adenosine diphosphoribose incorporation, glucose-6-phosphate dehydrogenase, cyclic-AMP phosphodiesterase, and glutathione reductase. However, striking differences of activity were found for the enzyme, NAD(P) glycohydrolase (EC 3.2.2.6). Activity levels were, as a mean, four times higher in Burkitt's lymphoma-derived cell lines than in nonmalignant control lines, and the difference was highly significant (p less than 0.02). All Burkitt cell lines containing translocations of chromosome 8 with either chromosomes 2, 14 or 22 showed an increased activity. The specificity and significance of this possible enzymatic marker of Burkitt's lymphoma cells is discussed.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000201:enzymology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009244:NAD+ Nucleosidase
D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase