title: Construction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.
abstract: Bacteriophage Mu d1 (lac Aprr) was used to obtain strains of Escherichia coli K-12 in which the lac genes are expressed from the promoter of pheA, the structural gene for the enzyme chorismate mutase P-prephenate-dehydratase. A derivative of bacteriophage lambda which carries the pheA-lac fusion was prepared; the method used is generally applicable for the construction, from Mu dl lysogens, of specialized transducing lambda phage carrying the promoter-lac fusions. A restriction enzyme cleavage map of lambda ppheA-lac for the enzymes HindIII and PstI is presented.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005796:Genes; D006836:Hydro-Lyases / Q000235:genetics*; D007763:Lac Operon; D008242:Lysogeny; D009876:Operon; D011302:Prephenate Dehydratase / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D006836:Hydro-Lyases; D011302:Prephenate Dehydratase