Generation of hydrogen peroxide during the oxidation of L-phenylalanine by Proteus mirabilis isolated membranes.
In the process of L-phenylalanine oxidation by Proteus mirabilis cytoplasmic membrane, hydrogen peroxide was produced at a rate corresponding to 1-3 per cent of the total electron flow (30-110 nmoles min-1mg-1). Peroxide was estimated using a fluorimetric assay with horseradish peroxidase, or by anodic oxidation on a platinum electrode. When using the former method, superoxide dismutase decreased the apparent yield of peroxide, a fact suggesting that H2O2 was in part the dismutation product of superoxide radicals. However the superoxide dismutase effect could be an artefact due to the generation of some superoxide during the peroxidatic reaction in the assay. Adrenaline was the reagent used for the detection of superoxide. There was no significant emergence of superoxide as the result of phenylalanine oxidation by the membrane (specific activity lower than 1-2 nmoles min-1mg-1). Thus it seemed that superoxide was not an intermediate for the bulk of H2O2 formed in this system. According to the results, peroxide was probably formed at a stage of electron transport earlier than the cytochrome level. The membrane phenylalanine dehydrogenase could be a site where peroxide was evolved in these experiments.
D000596:Amino Acids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017830:Octoxynol; D010084:Oxidation-Reduction; D010649:Phenylalanine / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011513:Proteus mirabilis / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D000596:Amino Acids; D013481:Superoxides; D011092:Polyethylene Glycols; D010649:Phenylalanine; D017830:Octoxynol; D006861:Hydrogen Peroxide; D002374:Catalase