Inhibitory effect of prostaglandin A2 on Na+-K+-ATPase activity in synaptic plasma membrane of rat brain in vitro.
1. The Na+-K+-ATPase activity in the synaptic plasma membrane of rat brain was inhibited by PGA2 in vitro. 2. PGA2 concentration required for 50% inhibition was 8.91 x 10(-5) M. 3. Inhibition mode of PGA2 for both ATP and Mg2+ was uncompetitive type. 4. PGA2 was competitive and noncompetitive with Na+ and K+, respectively. 5. The Ki values for PGA2 inhibition for ATP. Na+, K+ and Mg2+ were 6.0 x 10(-5), 2.7 x 10(-5), 10.8 x 10(-5) and 0.6 x 10(-5) M, respectively.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011454:Prostaglandins A / Q000494:pharmacology*; D051381:Rats; D012974:Sodium Iodide / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013570:Synaptic Membranes / Q000201:enzymology*
D011454:Prostaglandins A; D000254:Sodium-Potassium-Exchanging ATPase; D012974:Sodium Iodide