title: Growth and infectivity titration of virulent infectious bursal disease virus in established cell lines from lymphoid leukosis tumors.
abstract: Lymphoblastoid cell lines were investigated for susceptibility to virulent (wild) infectious bursal disease virus (IBDV) and attenuated IBDV by detection of fluorescent antigen in inoculated cells. LSCC-BK3 and LSCC-CU10 cells were susceptible to both types of IBDV; LSCC-1104-B-1 line was susceptible to only the attenuated IBDV. However, 2 lymphoid leukosis and 2 Marek's disease cell lines were refractory to growth of any type of IBDV. When the J1 strain of virulent IBDV was inoculated into LSCC-BK3 cells, intracellular and extracellular viruses began to increase logarithmically between 4 and 8 hr postinoculation and reached 10(6.3) and 10(7.3) TCID50/0.1 ml, respectively, at 72 hr. Infectivity titration and serum-neutralization tests of virulent IBDV were possible with LSCC-BK3 cells.
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius; D002460:Cell Line; D002645:Chickens; D007243:Infectious bursal disease virus / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D008223:Lymphoma / Q000382:microbiology / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology; D012087:Reoviridae / Q000254:growth & development*; D013160:Splenic Neoplasms / Q000382:microbiology / Q000662:veterinary; D014774:Virulence; D014779:Virus Replication