Structure and variation of human ribosomal DNA: the external transcribed spacer and adjacent regions.
A group of human ribosomal DNA (rDNA) recombinants that include the probable site for initiation of transcription have been examined for sequence polymorphism. A detailed restriction map of one rDNA insert was constructed using plasmid subclones and end-labeled segments. Comparison of 16 similar rDNA inserts by restriction and heteroduplex analysis demonstrated striking conservation of the external transcribed spacer and 18S gene regions, but defined a region where restriction sites for the enzymes Sma I, Hpa II, and Hha I become frequent or variable. This region extends for about 400--800 base pairs (bp) at the left end of the rDNA insert and is postulated to contain nontranscribed spacer sequences. The use of cloned rDNA segments as probes for the restriction analysis of genomic rDNA has demonstrated certain fixed sites in the nontranscribed spacer that do not vary significantly among different individuals or tumor cell lines. In contrast, restriction with the enzyme Sal I reveals several variable fragments, one of which has been found only in a retinoblastoma cell line.
D001482:Base Composition; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis*; D004275:DNA, Ribosomal; D015253:Deoxyribonucleases, Type I Site-Specific; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D011110:Polymorphism, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D004262:DNA Restriction Enzymes; C030431:endodeoxyribonuclease EcoBI; D015253:Deoxyribonucleases, Type I Site-Specific