[Interaction of a cAMP-deficient Yersinia pseudotuberculosis mutant with a macrophage culture in vitro].
The behavior of a virulent Y. pseudotuberculosis strain and its c-AMP-deficient mutant possessing lower virulence in their in vitro interaction with the culture of peritoneal macrophages of white mice, as well as a change in the level of c-AMP in macrophages in the process of the phagocytosis of the initial and mutant strains were studied. The c-AMP-deficient mutant was shown to retain its ability to infect macrophages and to multiply in them, while loosing its cytotoxic effect. Macrophages obtained from mice previously immunized with the mutant acquired the capacity of resisting the cytotoxic action of the initial virulent strain. The mutant proved to be unable to elevate the level of c-AMP in macrophages in the process of phagocytosis, while the initial strain caused a considerable increase in the level of c-AMP in macrophages.
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D007114:Immunization; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D010587:Phagocytosis; D014774:Virulence; D015007:Yersinia / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*
D000242:Cyclic AMP
Vzaimodeĭstvie tsAMP-defitsitnogo mutanta Yersinia pseudotuberculosis s kul'turoĭ makrofagov in vitro.