Biological and immunological characterization of iodinated bovine growth hormone.
Iodinated bovine growth hormone, containing no more than 1 g-atom of iodine per mole of hormone is generally used as a tracer in studies related to the action and metabolism of the hormone. This derivative was tested in different biological and immunological systems in which the hormone is known to be active. The iodinated derivative was almost indistinguishable in its properties from the native hormone when it was examined by the following criteria: body growth promoting activity, rat liver uptake in vivo, binding to rabbit liver microsomes and primary antigen-antibody interactions. Micro-complement fixation experiments suggested that the iodination produces minor alterations in the affinity of some antigenic determinants.
D000818:Animals; D001681:Biological Assay; D001692:Biological Transport; D001835:Body Weight / Q000187:drug effects; D002417:Cattle; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000187:drug effects; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000378:metabolism; D006384:Hemagglutination; D007016:Hypophysectomy; D007457:Iodine Radioisotopes; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011986:Receptors, Somatotropin
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011986:Receptors, Somatotropin; C035648:somatotropin, iodinated; D013006:Growth Hormone