Effect of aldosterone on the human erythrocyte sodium--potassium pump in vitro.
1. The effects of aldosterone in vitro on the Na+, K+-dependent ATPase activity of isolated human erythrocyte membranes and on rubidium (86Rb) uptake and [3H]ouabain binding of intact erythrocytes were studied. 2. ATPase activity was nearly doubled (0.061 +/- 0.006 to 0.110 +/- 0.01 mumol of Pi h-1 mg-1 of protein) by the addition of a physiological concentration of aldosterone (2.7 X 10(-10) mol/l). Higher concentrations had no greater effect. 3. Aldosterone had no significant effect on 86Rb uptake or [3H]ouabain binding. 4. Erythrocytes contain aldosterone at concentrations similar to that in plasma. The effect of aldosterone on ATPase is probably maximal.
D000328:Adult; D000368:Aged; D000450:Aldosterone / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010042:Ouabain / Q000097:blood; D012413:Rubidium / Q000097:blood; D012964:Sodium / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood
D000450:Aldosterone; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium