title: Adaptation of an automated microbiology system for the growth of anaerobes and performance of antimicrobial susceptibility tests.
abstract: The authors examined the feasibility of growing anaerobes in an automated microbiology system (MS-2) and using the system for antimicrobial susceptibility testing. A total of 78 of 100 clinical anaerobic isolates grew in the MS-2 if 100 microL of an undiluted overnight suspension of organisms was inoculated into at least 2.5 mL of freshly prepared Wilkins-Chalgren broth supplemented with 0.07% agar. Susceptibility test results were determined with 50 anaerobes tested against seven antibiotics in the MS-2 system and were compared with results determined with a qualitative reference susceptibility test method. of the 350 tests, 88% of the MS-2 results agreed with the qualitative reference results. False-susceptible and false-resistant results were reported for 8.6 and 3.4%, respectively, of the MS-2 results.
mesh_terms: D000190:Actinomyces / Q000254:growth & development; D000693:Anaerobiosis; D001431:Bacteriological Techniques; D001441:Bacteroides fragilis / Q000254:growth & development*; D003016:Clostridium perfringens / Q000254:growth & development*; D003201:Computers; D003470:Culture Media; D006801:Humans; D007185:Incubators; D008826:Microbial Sensitivity Tests / Q000379:methods; D010460:Peptococcus / Q000254:growth & development*
chemical_list: D003470:Culture Media