Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10.
We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.
D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D009154:Mutation; D010957:Plasmids; D013045:Species Specificity
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes