title: Transcriptional control signals of a eukaryotic protein-coding gene.
abstract: Transcriptional control signals of a model eukaryotic protein-coding gene have been identified by a new procedure of in vitro mutagenesis. This method allows small clusters of nucleotide residues to be substituted in a site-directed manner without causing the addition or deletion of other sequences. Transcription assays of a systematic series of these clustered point mutants have led to the identification of three distinct control signals located within the 105-nucleotide residues immediately upstream from the point where transcription begins.
mesh_terms: D001483:Base Sequence; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D009154:Mutation; D012333:RNA, Messenger / Q000032:analysis; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D012333:RNA, Messenger; D013937:Thymidine Kinase