Induction of Epstein-Barr virus by an new tumor promoter, teleocidin, compared to induction by TPA.
The effect of teleocidin, a new, naturally occurring tumor promoter on induction of Epstein-Barr virus (EBV), was compared with that of a known tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Early antigen (EA) and/or capsid antigen (VCA) of EBV was induced in the EBV genome-carrying cell lines C-6 and P3HR-I cells by teleocidin, its effect being maximal at a concentration of 12.5 ng/ml. The production of infectious EBV from P3-HR-I cells was enhanced by teleocidin maximally at a concentration of 0.5 to 2.5 ng/ml. The outgrowth of EBV-transformed cells from peripheral lymphocytes of seropositive healthy donors was also enhanced by teleocidin at a concentration of 0.02 to 0.5 ng/ml. TPA tested simultaneously in all experiments exhibited the same activities as teleocidin, and was effective at similar concentrations. Teleocidin enhanced both EA and VCA synthesis in P3HR-I cells additively with n-butyrate, but not with TPA. This suggests that teleocidin and TPA have a common mechanism of action, although their chemical structures are different.
D000470:Alkaloids / Q000494:pharmacology*; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D004305:Dose-Response Relationship, Drug; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D008235:Lyngbya Toxins; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D014775:Virus Activation / Q000187:drug effects*
D000470:Alkaloids; D000956:Antigens, Viral; D008235:Lyngbya Toxins; D010704:Phorbols; C022176:teleocidins; D013755:Tetradecanoylphorbol Acetate