title: Lyt phenotype of H-2b CTL effectors and precursors specific for the SV40 transplantation rejection antigen.
abstract: The immune effector cells mediating the in vitro immune response to the SV40 transplantation rejection antigen were characterized by using monoclonal antibody directed against lymphocyte differentiation (Lyt) antigens. Two distinct T lymphocyte populations were found to be responsible for the in vitro lysis of SV40-transformed cells, and Lyt 1+,2+ cytotoxic T lymphocyte (CTL) that is present in the spleens of SV40 immunized mice 9 days postimmunization and an Lyt 1-,2+ CTL that is generated by secondary in vitro stimulation of in vivo primed spleen cells. During secondary in vitro stimulation of SV40 immune spleen cells obtained 9 days postimmunization, a shift in the Lyt phenotype of the CTL from Lyt 1+,2+ to Lyt 1-,2+ is observed. Although the Lyt 1-,2+ CTL can be derived from Lyt 1-,2+ noncytotoxic memory cells, it is not known whether the Lyt 1+,2+ CTL differentiates into an Lyt 1-,2+ CTL during in vitro stimulation.
mesh_terms: D000950:Antigens, Ly / Q000032:analysis*; D000956:Antigens, Viral; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007156:Immunologic Memory; D013539:Simian virus 40 / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000950:Antigens, Ly; D000956:Antigens, Viral