title: Production of human monoclonal antibody to X31 influenza virus nucleoprotein.
abstract: In vitro stimulation of human peripheral blood mononuclear cells with X31 influenza virus antigen has been used to enrich for specific anti-X31 antibody-producing cells. Following Epstein-Barr virus transformation of these stimulated cells, a cell line which produces human antibody to X31 virus was derived and subsequently cloned. The cloned cells secrete and IgGl kappa antibody which is directed against the nucleoprotein of A type influenza virus. Culture supernatants contain 10 to 20 micrograms/ml of specific antibody which is now used as a standard for the ELISA assay used in our laboratory to detect antibodies to influenza virus.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D008213:Lymphocyte Activation; D009698:Nucleoproteins / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D014764:Viral Proteins