title: Herpes simplex virus types 1 and 2 induce shutoff of host protein synthesis by different mechanisms in Friend erythroleukemia cells.
abstract: Herpes simplex virus type 1 (HSV-1) and HSV-2 disrupt host protein synthesis after viral infection. We have treated both viral types with agents which prevent transcription of the viral genome and used these treated viruses to infect induced Friend erythroleukemia cells. By measuring the changes in globin synthesis after infection, we have determined whether expression of the viral genome precedes the shutoff of host protein synthesis or whether the inhibitor molecule enters the cells as part of the virion. HSV-2-induced shutoff of host protein synthesis was insensitive to the effects of shortwave (254-nm) UV light and actinomycin D. Both of the treatments inhibited HSV-1-induced host protein shutoff. Likewise, treatment of HSV-1 with the cross-linking agent 4,5',8-trimethylpsoralen and longwave (360-nm) UV light prevented HSV-1 from inhibiting cellular protein synthesis. Treatment of HSV-2 with 4,5',8-trimethylpsoralen did not affect the ability of the virus to interfere with host protein synthesis, except at the highest doses of longwave UV light. It was determined that the highest longwave UV dosage damaged the HSV-2 virion as well as cross-linking the viral DNA. The results suggest that HSV-2 uses a virion-associated component to inhibit host protein synthesis and that HSV-1 requires the expression of the viral genome to cause cellular protein synthesis shutoff.
mesh_terms: D000818:Animals; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D005814:Genes, Viral; D005914:Globins / Q000096:biosynthesis*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D018139:Simplexvirus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014307:Trioxsalen / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D003609:Dactinomycin; D005914:Globins; D014307:Trioxsalen