Localization of membrane proteins by the use of a photoreactive fatty acid incorporated in vivo into vesicular stomatitis virus.
Vesicular stomatitis virus grown in the presence of omega-[9-3H]diazirinophenoxy nonanoate resulted in the biosynthetic incorporation of this photoreactive fatty acid into viral phospholipids as well as into the membrane anchoring domain of the viral G glycoprotein. Photolysis of the isolated virus at 360 nm resulted in extensive labeling of the G protein but none of the other proteins by the viral phospholipids. In addition, a new product was obtained and was identified as a G-G dimer by its molecular weight and its reaction with anti-G antibody. These results demonstrate the use of photoreactive fatty acid to identify integral membrane proteins and to make photoaffinity probes of fatty acylated membrane proteins.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism*; D007739:L Cells; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D051379:Mice; D010782:Photolysis; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D005227:Fatty Acids; D007202:Indicators and Reagents; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014316:Tritium; C037203:omega-(diazirinophenoxy)nonanoic acid