Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
The position of the yeast phosphoglycerate kinase (PGK) gene has been mapped on a 2.95kb Hind III fragment. We have determined the nucleotide sequence of the 5' flanking region and compared this sequence with those from 16 other yeast genes. PGK, like all other yeast genes has an adenine residue at position -3. It has two possible TATA boxes at positions -114 and -152 and a CAAT box at -129. In addition we have defined a structure at position -63 to -39 that is common to all yeast genes that encode an abundant RNA. This structure is a CT-rich block followed, about 10 nucleotides later, by the sequence CAAG.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics*; D005796:Genes; D009693:Nucleic Acid Hybridization; D009876:Operon; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013045:Species Specificity; D014158:Transcription, Genetic
D004271:DNA, Fungal; D010735:Phosphoglycerate Kinase; D004262:DNA Restriction Enzymes