Evidence that the insulin secretagogue, beta-cell-tropin, is ACTH22-39.
The pituitary neurointermediate lobe of genetically obese (ob/ob) mice contains a hormone which stimulates insulin release and which cross-reacts with a -COOH-terminal ACTH antiserum, suggesting that it is related to corticotropin-like intermediate lobe peptide (CLIP), the 18-39 fragment of ACTH. The hormone, which we have called beta-cell-tropin, has been shown to be present in the plasma of the ob/ob mouse and to potentiate glucose induced insulin secretion. We have now shown that ACTH22-39 prepared by tryptic digestion of human synthetic CLIP behaves similarly on Biogel chromatography and on reverse-phase HPLC to the naturally occurring beta-cell-tropin. Furthermore, beta-cell-tropin and ACTH22-39 have indistinguishable antigenic and insulin releasing properties.
D000324:Adrenocorticotropic Hormone / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D053497:Corticotropin-Like Intermediate Lobe Peptide; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D051379:Mice; D008820:Mice, Obese; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D010902:Pituitary Gland / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism
D007328:Insulin; D010446:Peptide Fragments; D053497:Corticotropin-Like Intermediate Lobe Peptide; C026834:beta-cell tropin; D000324:Adrenocorticotropic Hormone; D014357:Trypsin