title: [Devitalization and haemostasis by thermal destruction--results of enzyme-histochemical and histological examination of oviduct specimens, following tissue coagulation by means of endocoagulation or high-frequency current (author's transl)].
abstract: Semm's technique of endocoagulation has been used by the authors for haemostasis in endoscopic abdominal surgery at the Department of Gynecology and Obstetrics, University of Kiel since 1973. --Enzyme-histochemical and histological tests, all based on endocoagulation or high-frequency current procedures, were applied to 100 human fallopian tubes. Fifteen of them remained uncoagulated and were used for comparison. --Membrane ATPase, 5-nucleotidase, cytochrome-C-oxydase, and other essential enzymes in cellular metabolism were inactivated by temperatures in excess of 57 degrees C. These enzymes, consequently, were no longer detectable from enzyme-histochemical preparations, whereas active tissue regions, those which still contained vital cells, were stained black to brown. --Negative enzyme reactions occurred in response to a coagulation forceps temperature of 120 degrees C, when applied not less than 20 seconds. Residual enzyme activities were still recordable from certain tubal areas, when forceps temperatures below 120 degrees C had been used. In such cases coagulation was insufficient, particularly in the inner layers of tubes (epithelium, mucosa). Temperatures above 130 degrees C and coagulation lengths of more than 20 seconds proved to be unnecessary, since no improvement in results was thus achievable. Temperatures of 140 degrees C and more have changed coagulated tissue into "adhesive" and make it stick to the coagulation forceps. Instrument withdrawal can in such cases cause bleeding rather than the desired haemostatic effect.
mesh_terms: D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000378:metabolism; D004564:Electrocoagulation; D003576:Electron Transport Complex IV / Q000378:metabolism; D005187:Fallopian Tubes / Q000201:enzymology*; D005260:Female; D006489:Hemostatic Techniques; D006358:Hot Temperature; D006801:Humans; D007044:Hysterectomy / Q000379:methods; D009708:Nucleotidases / Q000378:metabolism; D014022:Tissue Survival
chemical_list: D003576:Electron Transport Complex IV; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases
vernacular_title: Devitalisierung und Hämostase durch destruktive Wärme.