Induction of melanized cells from a goldfish erythrophoroma: isolation of pigment translocation variants.
Melanization was induced in some cells of a goldfish tumor cell line (GEM-81) by cultivating the cells in autologous serum. The melanized cells continued to proliferate in vitro and several clones were isolated that differed with respect to cell morphology and intracellular distribution of pigment. Some of the clones consisted of cells able to translocate their melanosomes in response to epinephrine, melatonin, or adenosine 3', 5'-monophosphate.
D000818:Animals; D002454:Cell Differentiation; D002999:Clone Cells / Q000166:cytology; D000242:Cyclic AMP / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006054:Goldfish; D008543:Melanins / Q000096:biosynthesis*; D008547:Melanophores / Q000187:drug effects / Q000648:ultrastructure*; D008550:Melatonin / Q000494:pharmacology; D009068:Movement / Q000187:drug effects; D009374:Neoplasms, Experimental / Q000473:pathology*
D008543:Melanins; D000242:Cyclic AMP; D008550:Melatonin; D004837:Epinephrine