A hybridoma cell line secreting antibody to poliovirus type 3 D-antigen: detection in virus harvest of two D-antigen populations.
A mouse hybridoma cell line (Mo56) secreting IgG antibody to poliovirus type 3 D-antigen was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with Saukett virus. The monoclonal antibody was specific for Saukett virus strains in virus-neutralization and single-radial-diffusion tests. In immunoprecipitation tests the monoclonal antibody reacted with intact infectious virus particles (155S) and with a previously undescribed 70S poliovirus particle with D-antigenic reactivity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D017955:Poliovirus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007074:Immunoglobulin G