High-performance liquid chromatography of fat-soluble vitamins: separation and identification of vitamins D2 and D3 and their isomers in food samples in the presence of vitamin A, vitamin E and carotene.
Vitamins D2 and D3 and their corresponding previtamins and provitamins were resolved by reversed-phase high-performance liquid chromatography using a ternary solvent system (acetonitrile-methanol-water) pumped according to a gradient elution programme. The D vitamins were also resolved in the presence of other lipid-soluble vitamins (A, E and K1) and carotene. The peaks were monitored with a UV-visible variable-wavelength detector and were detected at their maximum absorbance, resulting in maximum sensitivity. Lipid-soluble vitamins and carotene were resolved in extracts obtained from oils and butter, thus permitting their identification in a single chromatographic run.
D002079:Butter / Q000032:analysis*; D002338:Carotenoids; D002762:Cholecalciferol / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004872:Ergocalciferols / Q000031:analogs & derivatives / Q000302:isolation & purification*; D005504:Food Analysis; D009821:Oils / Q000032:analysis*; D014801:Vitamin A; D014810:Vitamin E; D014812:Vitamin K
D004872:Ergocalciferols; D009821:Oils; D014801:Vitamin A; D014812:Vitamin K; D014810:Vitamin E; D002762:Cholecalciferol; D002338:Carotenoids; D002079:Butter