title: The nucleotide sequence of myosin light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue.
abstract: The nucleotide sequence of a cDNA clone (pML10) for chicken cardiac myosin light chain is described. The cDNA insert contains 613 nucleotides representing the entire coding sequence, with the exception of nine NH2-terminal amino acids, and the full 3'-non-coding region of 146 nucleotides. The missing 5' terminus of the mRNA, not represented in the clone pML10, was obtained by extension of the cDNA using a 43 nucleotide long internal EcoR1 fragment as a primer. The non-coding region contains several direct and inverted repeated sequences and the polyadenylation signal sequence AATAAA. The coding portion exhibits non-random usage of synonymous codons with a strong bias for codons ending in G and C.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002642:Chick Embryo; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D009218:Myosins