title: In vitro infection of fractionated chicken lymphocytes by infectious bursal disease virus.
abstract: Lymphocytes from bursa of Fabricius and thymus of chickens were purified and separated into the three cell subsets--T, B, and null cells--by the techniques of nylon fiber columns and cytotoxicity tests. The in vitro susceptibility of the fractionated lymphocytes to a virulent strain of infectious bursal disease virus (IBDV) was studied by using immunofluorescence as the infection criterion. B cells were highly susceptible. By contrast, T cells and null cells were insusceptible to infection by IBDV. The relationship between the target cells for virus infection and those B cells that possessed surface immunoglobulin (SIg) was tested. B cells were further divided into SIg(M)- and SIg(G)-bearing cells by immunoadsorbent columns employing anti-immunoglobulin M(IgM) (mu-specific) or anti-IgG (gamma-specific) sera coated with Sephadex. The SIg(M)-bearing cells were highly susceptible. These results suggest strongly that SIg(M)-bearing B cells were the target cells for infection by IBDV.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000382:microbiology*; D002060:Bursa of Fabricius / Q000166:cytology; D002478:Cells, Cultured; D002645:Chickens; D005455:Fluorescent Antibody Technique; D007243:Infectious bursal disease virus / Q000254:growth & development* / Q000276:immunology; D008215:Lymphocytes, Null / Q000382:microbiology*; D012087:Reoviridae / Q000254:growth & development*; D013601:T-Lymphocytes / Q000382:microbiology*; D013950:Thymus Gland / Q000166:cytology; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral