Use of an electroimmunodiffusion method on cellulose acetate films for the detection of antigenicity in human breast cancers that is related to the major core antigen of mouse mammary tumor virus (MuMTV).
A highly sensitive electroimmunodiffusion method on cellulose acetate films, developed by Abelev on the basis of isotachoelectrophoresis, was applied to search of MuMTV-related antigenicity in tumor tissue and sera of breast cancer patients. In 2 out of 5 breast cancer extracts, protein fractions corresponding to a molecular weight of about 50,000 daltons were seen to exhibit antigenicity related to the major core antigen of MuMTV (p27). This antigenicity could not be detected in the sera of the patients.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology*; D002482:Cellulose / Q000031:analogs & derivatives; D003429:Cross Reactions; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis; D014020:Tissue Extracts
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D009363:Neoplasm Proteins; D014020:Tissue Extracts; C005062:acetylcellulose; D002482:Cellulose