Effect of Bordetella pertussis on alpha 1 and beta-adrenergic and cholinergic muscarinic receptors in guinea pig lung membranes.
After intraperitoneal injection of Bordetella pertussis vaccine to guinea pigs, the alpha 1- and beta-adrenergic and cholinergic muscarinic receptors of the whole lung were measured by binding assays with the radioisotope-labeled antagonists, 3H-prazosin, 1-(3)H-dihydroalprenolol and 1-(3)H-quinuclidinyl benzilate, respectively. Injection of guinea pigs with pertussis vaccine resulted in an increase in the maximum binding (Bmax) of 3H-prazosin, while there was about a 30% reduction in 1-(3)H-dihydroalprenolol binding to beta-adrenergic receptors. No difference was observed in the dissociation constant (KD) for binding of each ligand to alpha 1- and beta-adrenergic receptors. 1-(3)H-quinuclidinyl benzilate binding indicated that the Bmax and KD for cholinergic muscarinic receptor in pertussis-sensitized guinea pigs were not significantly different from those normal control animals.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D006168:Guinea Pigs; D008168:Lung / Q000032:analysis / Q000294:innervation*; D008297:Male; D010567:Pertussis Vaccine / Q000494:pharmacology*; D011941:Receptors, Adrenergic / Q000032:analysis*; D011942:Receptors, Adrenergic, alpha / Q000032:analysis*; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000032:analysis*; D011976:Receptors, Muscarinic / Q000032:analysis*
D010567:Pertussis Vaccine; D011941:Receptors, Adrenergic; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic