Separation of steroidal estrogens and their major unconjugated metabolites by high performance liquid chromatography.
A high performance liquid chromatographic method is described for the rapid, non-destructive separation of a number of physiologically important steroidal estrogens, including the labile catechol estrogens. This procedures uses a "Diol" column and gradient elution to separate in a single run, estrogens ranging from 2-methoxy estrone, one of the least polar C18 steroids, to estriol, one of the most polar. Simpler, isocratic conditions, are provided for the separation of estrogens of similar polarity. A semi-preparative column of similar composition was used for the purification of samples containing 25 to 50 mg of individual steroids.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004958:Estradiol / Q000031:analogs & derivatives / Q000302:isolation & purification; D004967:Estrogens / Q000302:isolation & purification* / Q000378:metabolism; D002393:Estrogens, Catechol / Q000302:isolation & purification*; D006894:Hydroxyestrones / Q000302:isolation & purification
D004967:Estrogens; D002393:Estrogens, Catechol; D006894:Hydroxyestrones; C026418:4-hydroxyestrone; D004958:Estradiol; C001390:2-hydroxyestradiol; C014036:4-hydroxyestradiol; C024980:2-hydroxyestrone