Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus.
A temperature-sensitive (ts) mutant, GA 907/7, was isolated after mutagen treatment of avian myeloblastosis virus. When bone marrow cells or secondary yolk sac macrophages were infected with GA 907/7, the expression of transformation was greatly reduced at 41 degrees C. The results of temperature-shift experiments suggest that in GA 907/7 the putative v-myb gene product is functional only at 35.5 degrees C. Moreover, when ts-induced transformed cells were shifted to 41 degrees C, a partial morphological conversion to macrophage-like cells was obtained, while the majority of the cells underwent senescence and lysis. No leukemia was obtained when GA 907/7 was injected in 1-day-old chickens. Finally, a continuous cell line releasing genetically stable mutant virus was obtained after transformation of secondary yolk sac cells.
D000818:Animals; D001354:Avian Leukosis Virus / Q000302:isolation & purification*; D009189:Avian Myeloblastosis Virus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002645:Chickens; D009154:Mutation; D010641:Phenotype; D013696:Temperature; D014779:Virus Replication