Binding and incorporation of lecithin-cholesterol vesicles to lymphocytes: a spin-label study.
When lecithin-cholesterol vesicles, containing the membrane-bound spin probe 3-doxyl-cholestane, were set in contact with mouse lymphocytes, the vesicles adsorbed to the cell and vesicle-membrane components were transferred to it. The spin probe was enzymatically reduced at the inside of the cell membrane. The spin-label method provided a means to determine quantitatively the extent of vesicles adsorption and vesicle-cell fusion by measuring the transfer of vesicles membrane material to the cell. This method, together with the reduction of spin label by the cell, allowed also a quantitative estimate to the extent of endocytosis during cell-liposome interaction.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D007700:Kinetics; D008081:Liposomes; D008214:Lymphocytes / Q000378:metabolism*; D008433:Mathematics; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism*; D013113:Spin Labels
D008081:Liposomes; D010713:Phosphatidylcholines; D013113:Spin Labels; D002784:Cholesterol