Effects of stimulation on the steroid profile formed by rat adrenal capsule tissue incubated in vitro.
A characteristic of the response of non-dispersed rat adrenal capsule tissue (mainly zona glomerulosa) to ACTH stimulation is that corticosterone and aldosterone production is increased whereas 18-hydroxy-deoxycorticosterone (18-OH-DOC) is not. The effects of potential second messengers on the steroid profile were compared with those of ACTH and K+ ions in adrenal capsule incubations. ACTH stimulated corticosterone, 18-hydroxycorticosterone and aldosterone, but not 18-OH-DOC. In contrast, K+ (5.9 mM) stimulated 18-OH-DOC as well as the other capsule products. Compared with controls incubated with EDTA, the addition of Ca2+ and Mg2+ ions invariably stimulated aldosterone and 18-hydroxycorticosterone, while the effects on corticosterone were variable, and 18-OH-DOC production was unaltered. Addition of dibutyryl cyclic AMP (1 mM and 0.3 mM) or cyclic GMP (1mM) stimulated all products. The results show that Ca2+ ions and dibutyryl cAMP may have slightly different effects in that the cyclic nucleotides can stimulate all products whereas Ca2+ and Mg2+ ions preferentially support aldosterone and 18-hydroxycorticosterone. However, none of the potential intracellular stimulants studied fully reproduce the characteristic response of non-dispersed tissue to ACTH in which the secretion of corticosterone and 18-hydroxy-DOC is disassociated.
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D006152:Cyclic GMP / Q000031:analogs & derivatives*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000305:Adrenal Cortex Hormones; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D000324:Adrenocorticotropic Hormone; D006152:Cyclic GMP; D008274:Magnesium; D002118:Calcium