title: The development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses.
abstract: Two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of MRC-5 and Vero cells, and Herpes simplex type 2 virus. Cell growth was similar in both systems giving yields of 14 X 10(5)/cm2 in microcarrier and 18 X 10(5)/cm2 in glass sphere cultures with yields of Vero cells being marginally better than MRC-5 cells. Virus yields were only slightly lower in these cultures than in small scale stationary cultures and confirmed the fact that MRC-5 cells produce twice as much HSV-2 as Vero cells, thus neutralising the growth advantage of these cells. Techniques for harvesting the virus in small volumes need to be improved in order to get a high titre suspension from the high capacity cultures.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002522:Chlorocebus aethiops; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005898:Glass; D006801:Humans; D007668:Kidney; D008863:Microspheres; D018139:Simplexvirus / Q000502:physiology*; D014779:Virus Replication