Reversible ultrastructural changes in human fibroblasts grown in hepes buffered MCDB-104 supplemented with human serum.
Prolonged maintenance of human dermal fibroblasts in MCDB-104 medium supplemented with pooled human serum or platelet factor deficient preparations of human serum led to appearance of a large number of membrane bound inclusions, resembling lysosomes, and proliferation of small, Golgi associated vesicles. These inclusions did not appear if the cells were grown in Dulbecco-Vogt's modification of Eagle's minimal essential medium or in minimal essential medium supplemented with the same human serum fractions. Cells that acquired inclusions during a 10 d incubation in MCDB-104 subsequently lost these inclusions when transferred to Dulbecco'Vogt's medium for 4 d. Similar reversal of effects of MCDB-104 was also produced by MCDB-104 buffered with bicarbonate instead of HEPES.
D000328:Adult; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D003593:Cytoplasm / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure*; D006056:Golgi Apparatus / Q000648:ultrastructure; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008074:Lipoproteins / Q000097:blood* / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000648:ultrastructure; D010455:Peptides / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor
D003470:Culture Media; D006133:Growth Substances; D008074:Lipoproteins; D010455:Peptides; D010982:Platelet-Derived Growth Factor