The roles of individual polyoma virus early proteins in oncogenic transformation.
The expression in normal rat cells of modified polyoma virus genomes, separately encoding large T, middle T or small T antigens, has allowed the investigation of the roles of these proteins in oncogenic transformation. Middle T is sufficient to transform cells of established lines but the transformants are serum dependent. Large T lacks intrinsic oncogenic potential but can relieve the serum dependence of normal and transformed cells. Middle T alone cannot transform primary rat embryo fibroblasts.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D000957:Antigens, Viral, Tumor; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003470:Culture Media; D004279:DNA, Viral / Q000235:genetics; D009857:Oncogenes; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology; D051381:Rats; D014764:Viral Proteins / Q000502:physiology*
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D003470:Culture Media; D004279:DNA, Viral; D014764:Viral Proteins