Effects of extracts of rat brain on the digitalis receptor.
There is a possibility that an endogenous substance exists which interacts with a ouabain binding site on Na+, K+-ATPase. Recently, several reports have appeared suggesting the presence of an endogenous digitalis-like substance in acid-acetone extracts of brain. We have demonstrated that in preparing an acid-acetone extract, peroxidized lipids and lysophospholipids are produced, both of which inhibit Na+, K+-ATPase, thereby complicating interpretation. Preliminary evidence suggests, however, that when rat brains are extracted with an aqueous-acetone mixture under nitrogen, a principle is obtained which specifically inhibits Na+, K+-ATPase.
D000818:Animals; D001923:Brain Chemistry; D005260:Female; D008054:Lipid Peroxides / Q000032:analysis; D008297:Male; D010042:Ouabain / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D014020:Tissue Extracts / Q000494:pharmacology
D008054:Lipid Peroxides; D010743:Phospholipids; D011955:Receptors, Drug; D014020:Tissue Extracts; C027082:digitalis receptor; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase