The filamentous phage (Ff) as vectors for recombinant DNA--a review.
Derivatives of filamentous phage, f1, fd, and M13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.
D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D013045:Species Specificity
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes