Human T cell hybridomas specific for Epstein Barr virus-infected B lymphocytes.
A mutant of the Jurkat human T lymphoblastoid cell line deficient in hypoxanthine phosphoribosyltransferase, and resistant to ouabain, was fused with peripheral blood T lymphocytes primed in vitro with Epstein Barr virus- (EBV) transformed autologous B lymphocytes. After selection of somatic cell hybrids and cloning, hybridoma cell lines were obtained that reacted with autologous EBV-infected B lymphocytes, as detected by the release of interleukin 2 into the culture medium. The hybridomas did not react with i) EBV-uninfected autologous or allogeneic B lymphocytes, ii) three out of four allogeneic EBV-transformed cell lines, or iii) two established EBV-negative B cell lines. These functional hybridomas may ultimately prove useful in dissecting the means by which human T lymphocytes recognize and regulate EBV infection in vivo.
D001402:B-Lymphocytes / Q000276:immunology*; D002459:Cell Fusion; D002460:Cell Line; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D007376:Interleukin-2