23,24,25-Trihydroxyvitamin D3, 24,25,26-trihydroxyvitamin D3, 24-keto-25-hydroxyvitamin D3, and 23-dehydro-25-hydroxyvitamin D3: new in vivo metabolites of vitamin D3.
Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions, the metabolites were identified as 23,24,25-trihydroxyvitamin D3, 24,25,26-trihydroxyvitamin D3, 24-keto-25-hydroxyvitamin D3 and 23-dehydro-25-hydroxyvitamin D3.
D000818:Animals; D002112:Calcifediol / Q000031:analogs & derivatives*; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002762:Cholecalciferol / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006887:Hydroxycholecalciferols / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet
D006887:Hydroxycholecalciferols; D002762:Cholecalciferol; C026582:25-hydroxy-24-oxocholecalciferol; C033630:24,25,26-trihydroxyvitamin D3; C033632:23-dehydro-25-hydroxyvitamin D3; C033629:23,24,25-trihydroxyvitamin D3; D002112:Calcifediol