title: The distal nephron in the chick embryo as a target tissue for 1-alpha-25-dihydroxycholecalciferol.
abstract: A histological and ultrastructural study as well as an autoradiographic analysis after injection of tritiated 1,25-dihydroxycholecalciferol (1,25-DHCC) were conducted on the distal convoluted tubules of chick embryos. Distal tubules in the embryo were shown to have the same spatial distribution as described for the adult kidney; they presented a convoluted portion located in the vicinity of the central intralobular veins and straight portions irradiating from this region towards the periphery. The epithelium in these tubules was well differentiated; its cells had numerous interdigitating folds in their lateral boundaries which were especially numerous at the basal ends. This device greatly increased the membrane surface available for interchange and was interpreted as an expression of active water and/or mineral transport. Nuclear concentration of radioactivity was found 2 h after injection of tritiated 1,25-DHCC in both the pars convoluta and the pars recta of the distal tubules. This concentration could be blocked by the previous administration of large amounts of nonradioactive 1,25-DHCC. These facts were interpreted as indicating that distal convoluted tubules in the chick embryo are functionally differentiated and contain target cells for 1,25-DHCC.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002117:Calcitriol / Q000502:physiology*; D002642:Chick Embryo / Q000502:physiology*; D007684:Kidney Tubules / Q000196:embryology*; D007686:Kidney Tubules, Distal / Q000196:embryology* / Q000502:physiology / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000502:physiology
chemical_list: D011956:Receptors, Cell Surface; D002117:Calcitriol