Flow cytofluorometric study of lipoprotein interactions with cultured endothelial cells.
Accumulation of rhodamine B isothyocyanate-conjugated low density lipoproteins (R-LDL) in cultured endothelial cells from human umbilical cord was studied with a fluorescence activated cell sorter. R-LDL uptake was blocked at 0 degrees C, inhibited by addition of excess of nonlabeled low density lipoprotein, high density lipoprotein-2 or high density lipoprotein-3. High density lipoprotein-2 was about twice as effective in inhibition of R-LDL uptake as high density lipoprotein-3. Endothelial cells that formed a contact-inhibited monolayer lost the ability to incorporate R-LDL via a receptor-mediated pathway. Using R-LDL, it was possible to distinguish cells with different levels of R-LDL incorporation.
D002469:Cell Separation; D002478:Cells, Cultured; D003260:Contact Inhibition; D004727:Endothelium / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011973:Receptors, LDL; D012235:Rhodamines
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D012235:Rhodamines; C027755:rhodamine isothiocyanate