A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels.
1. A low-pH lithium dodecyl sulphate/polyacrylamide-gradient slab-gel system, suitable for electrophoresis, is described, and the migration properties of standard proteins are compared on this and conventional high-pH gels. 2. Cytochrome oxidase may be partially resolved into its component polypeptides. The order of migration of these is, however, dependent on the pH of the gel system.
D003576:Electron Transport Complex IV / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005233:Fatty Alcohols; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011506:Proteins; D012967:Sodium Dodecyl Sulfate
D005233:Fatty Alcohols; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate; D003576:Electron Transport Complex IV