Multiplicity reactivation of alkylating agent damaged herpes simplex virus (type I) in human cells.
Herpes simplex virus type I (Strain KOS) is inactivated by treatment with MMS, MNNG and HN2 as determined by plaque assay on Vero cell monolayers, or by an infectious center assay with FS2 cells, human foreskin fibroblast line. At a given dose of MMS and MNNG, survival of the virus was significantly higher at a multiplicity of infection of 1.0 PFU/cell compared to 0.01 PFU/cell. These results indicate that HSV-1 infected human cells are capable of repairing chemically induced lesions by way of multiplicity reactivation. No evidence for multiplicity reactivation with HN2-treated virus could be obtained, however.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005347:Fibroblasts; D006801:Humans; D007668:Kidney; D008297:Male; D008466:Mechlorethamine / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000235:genetics; D012867:Skin
D008769:Methylnitronitrosoguanidine; D008466:Mechlorethamine; D008741:Methyl Methanesulfonate