Persistent activation of steroidogenesis in adrenocortical cells by photoaffinity labeling of corticotropin receptors.
Photolysis of rat adrenocortical cells in the presence of the photoreactive derivative [(2-nitro-5-azidophenylsulfenyl)Trp9]-adrenocorticotropic hormone (2,5-NAPS-ACTH) at 24 degrees C resulted in persistent activation of corticosterone production. The basal rate of steroidogenesis became maximal when photolysis was performed at 24 degrees C but remained the same as that of control cells when irradiation was performed at 0 degrees C. No increase in basal rate was observed with dark controls or cells photolyzed with [(2,4-dinitrophenylsulfenyl)Trp9]ACTH, a photoresistant analog of the hormone. Prephotolyzed 2,5-NAPS-ACTH failed to induce persistent activation. Both ACTH and 2,4-(dinitrophenylsulfenyl)Trp9-ACTH blocked the photo-induced activation of steroidogenesis elicited by 2,5-NAPS-ACTH. Under photolysis conditions which caused the basal rate of steroidogenesis to become maximal, a 3-fold increase in the basal rate of cAMP formation was observed.
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000096:biosynthesis*; D007700:Kinetics; D010782:Photolysis; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018043:Receptors, Corticotropin; D013696:Temperature
D011956:Receptors, Cell Surface; D018043:Receptors, Corticotropin; C026459:ACTH, 2-nitro-5-azidophenylsulfenyl-; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone