Enhanced production of hydroxyl radicals by the xanthine-xanthine oxidase reaction in the presence of lactoferrin.
The generation of hydroxyl radicals by the xanthine-xanthine oxidase reaction (C. Beauchamp and I. Fridovich (1970) J. Biol. Chem. 245, 4641-1616) has been shown to be increased by iron-saturated lactoferrin isolated from pig neutrophils. Hydroxyl radical production, measured by EPR spin trapping and by ethylene production from alpha-keto-gamma-methiol butyric acid, has been demonstrated to be produced by a Fenton-type Haber-Weiss reaction catalysed by lactoferrin. The possibility that lactoferrin catalyses such a reaction in vivo is considered.
D000818:Animals; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D007700:Kinetics; D007781:Lactoferrin / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D008892:Milk / Q000201:enzymology; D013481:Superoxides; D013552:Swine; D014969:Xanthine Oxidase / Q000378:metabolism*; D014970:Xanthines / Q000378:metabolism*
D005609:Free Radicals; D006878:Hydroxides; D007782:Lactoglobulins; D014970:Xanthines; D013481:Superoxides; D017665:Hydroxyl Radical; D014969:Xanthine Oxidase; D007781:Lactoferrin