Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase.
Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.
D000449:Aldehyde Reductase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D006600:Hexosephosphates / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism*; D007908:Lens, Crystalline / Q000201:enzymology*; D013058:Mass Spectrometry; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
D006600:Hexosephosphates; C035752:sorbitol 6-phosphate; D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C035751:sorbitol kinase