Overlapping genes at the DNA primase locus of the large plasmid ColI.
The sog gene of the large plasmid ColIdrd-1 has previously been shown to encode a DNA primase and a smaller antigenically related polypeptide. Genesis of these two products has been examined using Sog+ recombinant plasmids. Effects of amber mutations, isolated after in vitro mutagenesis, and deletions into or within sog suggest that the smaller polypeptide is a separate translation product which is encoded by DNA specifying the C-terminal region of the larger protein. Under control of the lac promotor, synthesis of both polypeptides is reduced when transcription is repressed. These findings imply that transcription of sog yields a single transcript which is translated from two initiation sites.
D000123:Acetyltransferases / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase; D019915:DNA Primase; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014176:Protein Biosynthesis; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*
D004274:DNA, Recombinant; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004262:DNA Restriction Enzymes