Influence of coenzyme A on the in vitro binding of benzo(a)pyrene metabolites to protein.
Incubation of 20,000 xg supernatants of liver homogenates from female Wistar rats with benzo(a)pyrene (BP) and other cofactors in the presence of coenzyme A (CoA) produced metabolites that required extensive hydrolysis successively in 2N NaOH, 2N HCl, 2N NaOH and 6N HCl for their release. It is suggested that coenzyme A activated benzpyrenyl-amino acid formed via epoxide(s). The activated amino acid(s) became bound to thiol protein.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D003065:Coenzyme A / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D013058:Mass Spectrometry; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D001580:Benzopyrenes; D001564:Benzo(a)pyrene; D003065:Coenzyme A