Effects of indomethacin on beta adrenoreceptor-stimulated renin release in the dog.
The hypothesis that prostaglandins participate in beta adrenoreceptor-stimulated renin release was tested by examining the effects of prostaglandin synthesis inhibition on renal nerve stimulation (0.5-5.0 Hz)-induced renin release. In phentolamine (5 micrograms/kg/min, intrarenal artery)-treated dogs (n = 6), renal nerve stimulation produced a frequency-related increase in renal renin secretion rate, without altering renal blood flow. In phentolamine plus propranolol (0.3 mg/kg i.v. bolus + 5 micrograms/kg/min infusion)-treated dogs (n = 6), renal nerve stimulation-induced renin release was inhibited by 86% (P less than .03), 75% (P less than .03) and 73% (P less than .02) at 1, 2 and 5 Hz, respectively. In contrast to propranolol, blockade of prostaglandin synthesis with indomethacin (8 mg/kg i.v. bolus) failed to alter the renin secretory response to renal nerve stimulation in phentolamine-treated animals. These results indicate that, in a situation of intrarenal alpha adrenoreceptor blockade, renal nerve stimulation induces renin secretion by a beta adrenoreceptor mechanism that is independent of prostaglandin biosynthesis.
D000818:Animals; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D008297:Male; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology*; D012079:Renal Circulation / Q000187:drug effects; D012083:Renin / Q000378:metabolism*
D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D012083:Renin; D007213:Indomethacin; D010646:Phentolamine