A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus.
The 3'-terminal labelling procedure for analyzing the genome segment profile of field isolates of rotavirus (Clarke and McCrae, 1981, J. Virol. Methods 2, 203) has been further developed to produce a rapid and sensitive method for generating diagnostic fingerprints from individual species of double-stranded RNA. The fingerprints were obtained by a one-dimensional resolution of overlapping terminally labelled oligonucleotides produced by partial nuclease digestion with a base-specific nuclease. This fingerprinting method should be of great value in characterizing the molecular details of genome segment variation and will facilitate detailed molecular epidemiological studies of this important virus group.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000235:genetics*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary; D006163:Ribonuclease T1; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D006163:Ribonuclease T1