title: Thyroglobulin interactions with thyroid membranes. Relationship between receptor recognition of N-acetylglucosamine residues and the iodine content of thyroglobulin preparations.
abstract: Bovine thyroglobulin has been subjected to sequential glycohydrolase treatment in order to define further the components of the carbohydrate chain which are important in binding of the glycoprotein to bovine thyroid membranes. Preparations of asialoagalactothyroglobulin exhibit the best binding, suggesting that exposed N-acetylglucosamine residues on the B carbohydrate chain of thyroglobulin play an important role in the interaction of thyroglobulin with the thyroid membranes. Enhanced binding of asialoagalactothyroglobulin to microsomal, lysosomal, and Golgi membranes, as well as to thyroid cells in culture, was also observed. Isopycnic rubidium chloride gradient centrifugation, a procedure used in the isolation of thyroglobulin molecules with a low iodine content, also isolates thyroglobulin molecules with a low sialic acid content and with an increased ability to interact with wheat germ agglutinin, a lectin which recognizes exposed N-acetylglucosamine residues. The studies further indicate that there is a correlation between iodine content, exposed N-acetylglucosamine residues, and the binding of thyroglobulin to thyroid membranes.
mesh_terms: D000117:Acetylglucosamine; D000818:Animals; D001667:Binding, Competitive; D002240:Carbohydrate Sequence; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D005944:Glucosamine / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D007454:Iodides / Q000032:analysis*; D007457:Iodine Radioisotopes; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013954:Thyroglobulin / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism*; D000519:alpha-Galactosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D007454:Iodides; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; C032198:thyroglobulin receptor; D013954:Thyroglobulin; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D005944:Glucosamine; D000117:Acetylglucosamine