title: Lymphocytes from ponies experimentally infected with equine herpesvirus 1: subpopulation dynamics and their response to mitogens.
abstract: Six pony foals, free of detectable serum neutralization (SN) antibody against equine herpesvirus type 1 by the standard virus-neutralization (VN) test, were inoculated with equine herpesvirus type 1. The ponies showed typical clinical signs of respiratory tract disease and developed a transient leukopenia, involving lymphocytes as well as neutrophils. The leukopenia reached its lowest point on postinoculation days (PID) 3 to 5 and then returned to base-line values by PID 8 to 10. On quantitation of lymphocyte subpopulations, T and B lymphocytes were decreased during the onset of leukopenia and then recouped during the recovery from leukopenia. However, the proportions of the T and B lymphocytes remained constant during the lymphopenia, ranging from 70% to 80% and 20% to 30%, respectively. The lymphocyte blastogenic response to mitogens increased to peak by PID 2 to 5 and then decreased to base line values or below by PID 7. Mitogen responses of T lymphocyte and mixed lymphocyte preparations were nearly similar. However, the responses of 2 ponies wee somewhat different from the responses of others in that there was an increase in the B lymphocytes in the range of 40% to 50% during the recovery phase of lymphopenia. Also, the 2 ponies' mixed lymphocyte response to mitogens was considerably higher and the T lymphocyte response to mitogen was lower as compared with that of mixed lymphocyte preparation. The importance of these 2 types of responses is discussed.
mesh_terms: D000208:Acute Disease; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses; D007958:Leukocyte Count / Q000662:veterinary; D008231:Lymphopenia / Q000276:immunology / Q000662:veterinary*; D009500:Neutralization Tests; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012141:Respiratory Tract Infections / Q000276:immunology / Q000662:veterinary*; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D000914:Antibodies, Viral; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A