title: The amino acid sequence of cytochrome c oxidase subunit VI from Saccharomyces cerevisiae.
abstract: The complete amino acid sequence of the nuclearly coded cytochrome c oxidase subunit VI was determined for a genetically defined haploid strain of Saccharomyces cerevisiae. The subunit contains 108 amino acids, has Mr = 12,627, is acidic (net charge of -9.7 at pH 7) and is quite polar (polarity index, 50.9%). Distribution of charges within the polypeptide chain is highly non-random. The NH2- and COOH-terminal regions are predominantly acidic whereas an apolar and a basic region are found in the interior, Subunit VI shows between 28 and 40% sequence homology (depending on the method of alignment) with subunit V of bovine cytochrome c oxidase; since the yeast subunit VI lacks methionine and contains only a single histidine residue very close to the NH2 terminus, it is unlikely that either of the two subunits carries heme alpha in the native enzyme.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000302:isolation & purification; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013045:Species Specificity; D014357:Trypsin
chemical_list: D046911:Macromolecular Substances; D010446:Peptide Fragments; D003576:Electron Transport Complex IV; D014357:Trypsin