Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells.
Using a sensitive and precise radioimmunoassay for human beta-endorphin, we have demonstrated the sustained secretion of opioid peptides from human pituitary tumour cells. Pituitary tumour tissue obtained from a patient with Nelson's syndrome was maintained in continuous monolayer culture and secreted both beta-lipotropin and beta-endorphin, with predominance of the latter. This is compatible with the idea that the beta-endorphin in normal human serum is secreted as such despite the predominance of beta-lipotropin compared with beta-endorphin in the anterior pituitary.
D002478:Cells, Cultured; D002850:Chromatography, Gel; D004723:Endorphins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008297:Male; D009347:Nelson Syndrome / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors; D001615:beta-Endorphin; D008083:beta-Lipotropin / Q000032:analysis / Q000378:metabolism
D004723:Endorphins; D001615:beta-Endorphin; D008083:beta-Lipotropin