title: Instability of the viral M protein in BHK-21 cells persistently infected with Sendai virus.
abstract: The study of viral protein expression in BHK cells persistently infected with Sendai virus showed that the viral M protein was greatly reduced in amount or absent in these cells. Pulse-chase experiments demonstrated that the M protein was synthesized at a normal rate, but was unstable compared to the other viral proteins. The M protein instability was independent of temperature and could account for part of the reduction in viral production by persistently infected cells. When a virus stock was grown in embryonated chicken eggs from viruses produced by persistently infected BHK cells, the M protein of this stock presented a restored stability in BHK cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003673:Defective Viruses / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D018184:Paramyxoviridae Infections / Q000378:metabolism*; D013696:Temperature; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014763:Viral Matrix Proteins; D014764:Viral Proteins