title: Stimulatory effect of pertussis toxin on tissue cyclic AMP levels in canine thyroid slices.
abstract: The in vitro effects of the protein purified from the culture medium of Bordetella pertussis (pertussis toxin) on cyclic AMP levels in canine thyroid slices were studied. The toxin caused a three-fold increase in tissue cyclic AMP levels after 18 h or longer incubation. Furthermore, when the slices were incubated with the toxin for 6 h and then incubated without the toxin for further 12 h, similar effects were observed. These results indicate that pertussis toxin elevates tissue cyclic AMP levels after a time lag in a manner different from other thyroid stimulators.
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001886:Bordetella pertussis; D000242:Cyclic AMP / Q000378:metabolism*; D004285:Dogs; D066298:In Vitro Techniques; D007700:Kinetics; D037342:Pertussis Toxin; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology; D010566:Virulence Factors, Bordetella
chemical_list: D001427:Bacterial Toxins; D010566:Virulence Factors, Bordetella; D013972:Thyrotropin; D000242:Cyclic AMP; D037342:Pertussis Toxin