Long-term preservation of transfecting activity of avian sarcoma proviral DNA.
The integrated proviral DNA of avian sarcoma virus (ASV) in host cell chromosomes has been isolated and stored in saline sodium citrate (SSC) solution or in 70% ethanol at 4 degrees C in a refrigerator over 4 years. This DNA was assayed by transfection of chick embryo cells(CEC). The biological activity of cellular transformation by the stored DNA was compared with that of a fresh isolate of the proviral DNA. The efficiency of the transfection by each DNA was almost the same.
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D051379:Mice; D011309:Preservation, Biological; D051381:Rats; D012034:Refrigeration; D013997:Time Factors; D014162:Transfection
D004279:DNA, Viral