Increased activity of polynucleotide ligase in 5-iodo-2'-deoxyuridine and mitomycin C-pretreated simian virus 40 (SV40)-infected monkey kidney cells.
The DNA ligase activity has been studied in 5-iodo-2'-deoxyuridine (IUdR), mitomycin C (MMC) and IUdR + MMC-treated SV40-infected or mock-infected CV1C11 monkey kidney cells. The results have shown that : 1. The level of enzyme activity is about two or three times higher in IUdR or MMC-treated non-infected cells; 2. SV40 infection doubles the level of ligase activity in the untreated cells; 3. There is an additional increase of ligase activity in IUdR or MMC-treated SV40-infected cells; 4. When infected or mock-infected cells are treated with IUdR + MMC, there is no modification of the results obtained with each drug alone; 5. Two peaks of different S values are detected in a partial purification of the drug(s) or viral induced enzyme(s). The increase in the ligase activity in drug-treated cells is independent of semi-conservative DNA synthesis. The drug(s) and viral-induced enzyme(s) is the consequence of a "de novo" synthesis of proteins, as shown by cycloheximide treatment.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D011088:DNA Ligases / Q000302:isolation & purification / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D007065:Idoxuridine / Q000494:pharmacology*; D007668:Kidney; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D011118:Polynucleotide Ligases / Q000302:isolation & purification / Q000378:metabolism*; D013539:Simian virus 40 / Q000201:enzymology*
D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D016685:Mitomycin; D011088:DNA Ligases; D011118:Polynucleotide Ligases; D007065:Idoxuridine