Regional localization of the genes for human HEXB. PGK, GALA. HPRT, G6PD by somatic cell hybridization.
Twenty independent man-mouse (Cl1D,LA/TK-, HPRT-) and man-hamster (CH,HPRT-) hybrids using female human cells with balanced reciprocal translocation XX,t(X;5)(q21;q11) were analyzed for human genes localized on chromosome 5 (HEXB), on chromosome X (PGK, GALA, HPRT, G6PD) and for the different chromosomes in relation with the balanced reciprocal translocation (chr.5, chr.5q-, chr.Xq+, chr.X). The different results obtained indicate that the genes for human markers HEXB, PGK are on Xq+, and that the genes for human markers GALA, G6PD are on 5q-. These data implicate finally the following localizations: HEXB on 5q11 leads to 5qter; PGK on Xq21 leads to Xpter; GALA, HPRT, G6PD on Xq21 leads to Xqter.
D000818:Animals; D002874:Chromosome Mapping; D002905:Chromosomes, Human, 4-5 / Q000648:ultrastructure*; D005260:Female; D005796:Genes; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006596:Hexosaminidases / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000235:genetics; D012730:Sex Chromosomes / Q000648:ultrastructure*; D014178:Translocation, Genetic; D014960:X Chromosome / Q000648:ultrastructure*; D000519:alpha-Galactosidase / Q000235:genetics
D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase; D006596:Hexosaminidases; D000519:alpha-Galactosidase