Collagenase activity in rabbit carcinoma: cell source and cell interactions.
Fibroblast-like cells (F-cells) and epithelial-like (E-cells) derived from cultures of rabbit VX-2 carcinoma released collagenase in both active and latent forms in serum-free medium at a level higher than that of normal rabbit fibroblast cultures. The enhanced capacity of the F-cells to release the enzyme, however, continued only for a few passages and then decreased significantly to a low level similar to that of the normal fibroblasts. The enzyme-release by the E-cells continued for a few more passages at a relatively moderate level, higher than that of normal fibroblasts. The release of collagenase in cultures of F-cells was enhanced by the presence of E-cells in mixed cultures as well as by medium conditioned by the E-cells type. Addition of cytochalasin B at 2 micrograms/ml did not significantly effect the enzyme activity released in the cultures. Serum from tumor-bearing rabbits appeared to stimulate the release of enzyme activity in cultures of either cell type.
D000818:Animals; D002277:Carcinoma / Q000201:enzymology*; D002450:Cell Communication; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D007700:Kinetics; D003012:Microbial Collagenase / Q000032:analysis*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology; D011817:Rabbits
D003571:Cytochalasin B; D003012:Microbial Collagenase