[Biochemical and ultrastructural research on the antagonism between vitamins A and D at the leve of the erythrocyte membrane].
The reason for the antagonistic action between retinol and cholecalciferol on cellular membranes was investigated "in vitro" on human erythrocytes, by testing their lipoperoxide production, the amount of membrane-bound retinol and the morphology of these cells by scanning electron microscope. Lipoperoxides were assayed by TBA-test; the membrane-bound retinol was tested by the Karr-Price method on lipids extracted from red blood cells by the Folch method and vitamin D-deprived by TCL chromatography; the morphological changes of these cells treated with vitamin A, or D, or A+D, were tested by scanning electron microscope. In our experimental system both retinol and cholecalciferol increase the lipoperoxide production from the erythrocytes; nevertheless no synergism of action can be demonstrated between the two vitamins. Cholecalciferol decreases the amount of membrane-bo und retinol as well as the changes produced by vitamin A in red blood cell surface. These results are in good agreement with our previous observations obtained by nuclear magnetic resonance, using the same experimental system.
D001667:Binding, Competitive; D002762:Cholecalciferol / Q000097:blood*; D004347:Drug Interactions; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D008855:Microscopy, Electron, Scanning; D014801:Vitamin A / Q000037:antagonists & inhibitors / Q000097:blood*
D008054:Lipid Peroxides; D014801:Vitamin A; D002762:Cholecalciferol
Ricerche biochimiche ed ultrastrutturali sull'antagonismo tra le vitamine A e D a livello della membrana degli eritrociti.