Identification and characterization of a DNase induced by Epstein-Barr virus.
The diterpene ester promoter of mouse skin tumors, 12-O-tetradecanoyl-phorbol-13-acetate, induced a DNase activity in the Epstein-Barr virus-producer cell line P3HR-1. The elution patterns of the enzyme from DEAE-cellulose, phosphocellulose, and DNA-cellulose columns were different from virus-associated DNA polymerase activity. The partially purified activity could be neutralized to the extent of 90% by sera of patients with nasopharyngeal carcinoma. Purified immunoglobulin G from sera of nasopharyngeal carcinoma patients inhibited this enzyme and that obtained from superinfected Raji cells to the same extent. The partially purified enzyme preferred native DNA as a substrate over denatured DNA and 3'-terminally labeled activated calf thymus DNA. The activity was inhibited by high ionic strength. Phosphonoformic acid did not have any effect on this enzyme activity.
D002051:Burkitt Lymphoma; D002460:Cell Line; D002472:Cell Transformation, Viral; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003851:Deoxyribonucleases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G; D007700:Kinetics; D009669:Nose Neoplasms / Q000097:blood; D010610:Pharyngeal Neoplasms / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007074:Immunoglobulin G; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D013755:Tetradecanoylphorbol Acetate