title: Functional properties of porcine-thyroid follicles in suspension.
abstract: Porcine thyroid follicle cells were isolated (about 10(7) cells per gram of tissue) and cultured in small aggregates in agarose-coated culture dishes. The aggregates became arranged into follicle-like structures capable of iodide uptake and organification. In the presence of TSH (0.2 mU/ml), the aggregation of follicles was enhanced, and iodide uptake as well as TSH-stimulated organification of iodide was increased compared with that in the control. In culture, the active iodide metabolism was gradually lost over a 7-day period. This was not due to a disappearance of the TSH-adenylate cyclase system, since cAMP production was retained and stimulated by TSH (half-maximal effect at about 1 mU/ml). Acutely TSH stimulated iodide efflux and iodide organification (half-maximal effect at about 20 microU/ml). The stimulatory effect on organification was transient: within an hour further organification proceeded as in the absence of hormone. The effects on efflux and organification were already maximal at low TSH concentrations, whereas cAMP production was stimulated with up to 50-fold higher TSH levels, i.e. the findings were typical of spare receptors. In the continued presence of epidermal growth factor, a potent mitogen for thyroid cells, the follicles increased in size and contained one single large lumen. Their capability to take up and organify iodide was reduced.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007454:Iodides / Q000378:metabolism*; D007700:Kinetics; D013552:Swine; D013961:Thyroid Gland / Q000033:anatomy & histology / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*
chemical_list: D007454:Iodides; D004815:Epidermal Growth Factor; D013972:Thyrotropin; D000242:Cyclic AMP