title: A basement membrane-associated glycoprotein from skeletal muscle.
abstract: We have isolated a major glycoprotein that appears to be associated with rat skeletal muscle basement membrane. We determined that the glycoprotein was part of the muscle cell surface complex when we found it to be enriched in preparations of muscle ghosts. We isolate the glycoprotein from homogenized muscle preextracted with 4 M and 8 M urea. It elutes as a major component in the presence of 8 M urea/50 mM 2-mercaptoethanol. Its apparent molecular weight on sodium dodecyl sulfate gels is 130,000. Amino acid analysis indicates that it is not a collagen but that it does contain small amounts of hydroxyproline and hydroxylysine. There may be collagenous domains in the glycoprotein molecule, for it is cleaved into three fragments by purified bacterial collagenase. Immunoperoxidase staining confirms that the 130,000-dalton protein is localized at the surface of adult skeletal muscle cells. It is probably a general basement membrane-associated glycoprotein because we found material immunologically cross-reactive with the muscle glycoprotein in basement membrane regions of kidney, liver, brain, and small intestine. We have shown the glycoprotein to be distinct from fibronectin, laminin, and types I, III, IV, and V collagens in enzyme-linked immunosorbent assays.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D005110:Extracellular Space / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D003012:Microbial Collagenase / Q000378:metabolism; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats
chemical_list: D006023:Glycoproteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D003012:Microbial Collagenase