title: The proton translocating ATPase responsible for urinary acidification.
abstract: H+ secretion by the turtle urinary bladder is produced by a proton pump located in the luminal membrane. We show that a microsomal fraction of these cells contains an electrogenic proton translocating ATPase that is inhibited by dicyclohexylcarbodiimide but not oligomycin or vanadate. On a sucrose density gradient, this ATPase co-migrated with luminal membranes labeled with concanavalin A, but was separate from a lysosomal marker. This enzyme is therefore the H+ ATPase that causes urinary acidification.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004563:Electrochemistry; D008861:Microsomes / Q000201:enzymology; D006180:Proton-Translocating ATPases; D011522:Protons; D014426:Turtles / Q000378:metabolism*; D001743:Urinary Bladder / Q000201:enzymology*
chemical_list: D011522:Protons; D004024:Dicyclohexylcarbodiimide; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases