Changes in prostaglandin levels in cultures of SV40 -transformed macrophage cell lines in relation to their phenotypic expression.
A radioimmunoassay was performed to determine the total amounts of the A, B, and E series prostaglandins (prostaglandins) in culture fluids of simian virus 40 (SV40)-transformed mouse clonal macrophages, line 28-12, and its subline, 28-12 (Ara). When the proportion of phagocytic cells in confluent 28-12 cell cultures increased, the prostaglandin levels in the culture fluids decreased. On the other hand, stably phagocytic 28-12 (Ara) cells, which were derived from 28-12 cells and which had a reduced growth rate, did not release prostaglandins under the usual culture conditions; however, when they were treated with lipopolysaccharide or streptococcal preparation OK-432, large amounts of prostaglandins were released. In contrast, nonphagocytic cell populations in the cultures of 28-12 cells were not responsive to the drug treatment. These results suggest that there is a correlation between the phenotypic change from the nonphagocytic to the phagocytic state and a decrease in prostaglandin levels in culture fluids, and indicate that phagocytic cells are responsive to prostaglandin inducers, whereas nonphagocytic cells are not.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis; D010641:Phenotype; D010844:Picibanil / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D011863:Radioimmunoassay; D013539:Simian virus 40
D008070:Lipopolysaccharides; D011453:Prostaglandins; D010844:Picibanil