A reproducible method for the optimum infection of human mononuclear cells and lymphoid cell lines by herpes simplex virus type 1.
Parameters for the infection of human mononuclear cells (MNC) with herpes simplex virus type 1 (HSV-1) were investigated and a procedure was established which resulted in a reproducible optimum number of cells expressing virus-specific cell surface antigens. The number of cells infected was independent of sex of the donor and independent of whether the donor was HSV-seropositive or -seronegative. On the average 18 +/- 6% of HSV-infected MNC from any given donor expressed HSV-specific cell surface antigens. When the standard procedure was applied to a variety of lymphoid cell lines, a high percentage of cells of both B and non-T/non-B lines expressed HSV-specific cell surface antigens, whereas T-cell lines appeared resistant to HSV infection.
D000914:Antibodies, Viral / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D012737:Sex Factors; D018139:Simplexvirus / Q000276:immunology / Q000502:physiology*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral