title: Sulfated galactosaminoglycans of bovine periodontal ligament. Evidence for the presence of two major types of hybrids but no chondroitin sulfate.
abstract: Sulfated galactosaminoglycans of mature bovine periodontal ligament were separated into four fractions by ethanol precipitation. Fractions I and II were dermatan sulfates with high contents of L-iduronate, but only small amounts of this hexuronic acid were present in fractions III and IV. Effects of digestion with testicular hyaluronidase or a periodate-alkali treatment showed that most if not all of the glycans in fractions I, II and III were hybrid chains containing both L-iduronate and D-glucuronate. The composition of fraction IV was less certain, but the chains strongly resembled fraction III hybrids in electrophoretic characteristics, not chondroitin sulfate. The total amount of the D-glucuronate-rich fractions III and IV in the ligament was similar to that of I plus II. In contrast, almost all of the sulfated galactosaminoglycans of mature skin were rich in L-iduronate. The more varied composition of the ligament glycosaminoglycans may be related to the mixed population of cells in this tissue.
mesh_terms: D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000032:analysis; D003871:Dermatan Sulfate / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007067:Iduronic Acid / Q000032:analysis; D010504:Periodic Acid / Q000494:pharmacology; D010513:Periodontal Ligament / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D005965:Glucuronates; D011134:Polysaccharides; D010504:Periodic Acid; D003871:Dermatan Sulfate; D007067:Iduronic Acid; D002809:Chondroitin Sulfates; C007815:galactosaminoglycan; D006821:Hyaluronoglucosaminidase