Further studies on the interaction between thrombin and GP Ib using crossed immunoelectrophoresis. Effect of thrombin inhibitors.
The platelet surface protein GP Ib (glycocalicin-related protein) has been shown to be retarded by thrombin-Sepharose 4B in a crossed immunoelectrophoresis system. The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D001974:Bromosuccinimide / Q000494:pharmacology; D002417:Cattle; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008565:Membrane Proteins / Q000097:blood*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D019038:Platelet Glycoprotein GPIb-IX Complex; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D006023:Glycoproteins; D008565:Membrane Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; C023592:glycocalicin; D014107:Tosyllysine Chloromethyl Ketone; D010664:Phenylmethylsulfonyl Fluoride; D013917:Thrombin; D001974:Bromosuccinimide