Potentiation of neutralization of Varicella-Zoster virus to antibody to immunoglobulin.
Antibody to varicella-zoster virus was measured in Vero cell cultures by a conventional and an enhanced plaque neutralization test. In the latter, heterologous antibody to human immunoglobulin was added to the virus-serum mixture to potentiate the neutralization reaction. The antibody to human immunoglobulin enhanced the sensitivity of the neutralization test seven- to 107-fold as compared with the conventional procedure. The test was highly reproducible in that individual serum titers fluctuated in a twofold range on repeated testing. A prozone phenomenon was observed in the enhanced neutralization test in low-titered sera, but not with high-titered sera or with specimens of cerebrospinal fluid. The specificity of the test was confirmed by the detection of seroconversion in recipients of live varicella virus vaccine.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000032:analysis* / Q000134:cerebrospinal fluid; D002460:Cell Line; D002644:Chickenpox / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D014645:Herpesvirus 3, Human / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007223:Infant; D009500:Neutralization Tests; D014611:Vaccination; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines