alpha-Adrenergic stimulation of glycolysis and Na+, K+-transport in perfused rat liver.
Interactions between alpha-adrenergic-induced lactate production, respiration and K+ uptake of perfused rat livers were investigated. Inhibition of mitochondrial electron transport by cyanide had no effect on K+ uptake, decreased phenylephrine-induced lactate production by 60% and suppressed extra O2 consumption by more than 90%. Ouabain did not affect alpha-adrenergic-induced respiration but inhibited K+ uptake and extra lactate production. Effects of ouabain and K+-free medium on lactate production were additive and independent of cyanide, indicating that they were mediated by extramitochondrial mechanisms. Extra ATP utilized for K+ uptake in response to phenylephrine was apparently of glycolytic rather than mitochondrial origin. We propose that alpha-adrenergic-induced lactate production is secondary to an enhanced operation of the plasma membrane (Na+,K+)-ATPase.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003486:Cyanides / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010656:Phenylephrine / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D003486:Cyanides; D007773:Lactates; D010656:Phenylephrine; D019344:Lactic Acid; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium