Effects of insulin on lipolysis and lipogenesis in hamster white adipocytes with high sensitivity to hormones.
A modified procedure for preparation of hamster adipocytes by collagenase digestion under carefully controlled conditions has been developed. The adipocytes were 4- to 8-fold more sensitive to catecholamine stimulation of lipolysis than cells prepared by a commonly used method (Hittelman, K.J., Wu, C.F. and Butcher, R.W. (1973) Biochim. Biophys. Acta 304, 188-196) and also more sensitive to the anti-lipolytic action of insulin. The effects of insulin on lipogenesis, measured as [3H]glucose conversion to cell lipids, and on catecholamine-stimulated lipolysis were compared under identical conditions with the same cell batch. Isoprenaline-stimulated lipolysis was found to be half-maximally inhibited by an insulin concentration 8-fold lower than that stimulating lipogenesis to a corresponding extent (half-maximal effects at insulin concentrations of 40 vs. 300 pM). A similar difference was found when cells had been stimulated with adrenaline instead of isoprenaline.
D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D008647:Mesocricetus; D003012:Microbial Collagenase / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology
D007328:Insulin; D008055:Lipids; D003012:Microbial Collagenase; D005947:Glucose; D007545:Isoproterenol; D009638:Norepinephrine