[Surface ultrastructural study on the human endometrial interstitium using digestion method (author's transl)].
A scanning electron microscopic study on the human endometrium, using Evan's method and modifications, revealed the following findings. a) 8N . HCl-Collagenase method: High-speed digestion of the specimen made the determination of the adequate digestion time difficult, due to conc. HCl and high processing temperature. b) 1N . HCl-Collagenase method: Long digestion time produced many artifacts as shrinkage of the cells or formation of small holes on them. c) Tripsin-Collagenase method: Tissue breaking often resulted before the adequate digestion. d) Hyaluronidase-Collagenase method: This is most satisfactory in classifying glands and vessels, and digestion time is the shortest of all methods.
D004717:Endometrium / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D003012:Microbial Collagenase; D008855:Microscopy, Electron, Scanning; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase