title: DNA of bovine herpesvirus type 1 in the trigeminal ganglia of latently infected calves.
abstract: Twelve calves infected with bovine herpesvirus type 1 (BHV-1) were killed when in a latent state of infection. Latency was verified 30 days after virus inoculation of the calves by seroconversion, absence of virus shedding, and in 2 calves, by recrudescence of the infection after they were treated with dexamethasone. By in situ hybridization techniques and autoradiography, DNA of BHV-1 was detected in 13 of 23 trigeminal ganglia of latently infected calves. Viral DNA was restricted to the nucleus of nerve cells. Single neurons harboring BHV-1 DNA were observed in 4.9% of the sections (n = 325) of the trigeminal ganglia. The results obtained correspond to those known from herpes simplex virus infections in mice. The implications for the virus-host relationship are discussed.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis*; D007242:Herpesvirus 1, Bovine / Q000032:analysis* / Q000276:immunology; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012668:Trigeminal Ganglion / Q000032:analysis*; D014276:Trigeminal Nerve / Q000032:analysis*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral