Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay.
A new physical method was developed to assay genetic recombination of phage T7 in vivo. The assay utilized T7 mutants that carry unique restriction sites and was based on the detection of a new restriction fragment generated by recombination. Using this assay, we reexamined the genetic requirements for recombination of T7 DNA. Our results were in total agreement with previous findings in that recombination required the products of genes 3 (endonuclease), 4 (primase), 5 (DNA polymerase), and 6 (exonuclease). Recombination was found to be independent of DNA ligase and DNA packaging and maturation functions.
D019915:DNA Primase; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D005092:Exonucleases / Q000378:metabolism; D005814:Genes, Viral; D008828:Microbiological Techniques; D009268:Nalidixic Acid / Q000494:pharmacology; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D011995:Recombination, Genetic; D013604:T-Phages / Q000235:genetics*
D004279:DNA, Viral; D009268:Nalidixic Acid; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004259:DNA-Directed DNA Polymerase; D004720:Endonucleases; D005092:Exonucleases; D004262:DNA Restriction Enzymes