A method for the purification of rotaviruses and adenoviruses from faeces.
Rotaviruses and non-cultivable enteric adenoviruses were purified from large volumes of faecal extracts using polyethylene glycol precipitation, centrifugation through 45% w/v sucrose and segregation by density in a cesium chloride density gradient. Yields were high and the preparations were pure enough to use in the production of type-specific antisera and in viral polypeptide analysis. Human rotavirus particles of density 1.38 g/ml were shown to be non-infectious for LLC-MK2 tissue culture cells.
D000260:Adenoviruses, Human / Q000302:isolation & purification* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002648:Child; D005243:Feces / Q000382:microbiology*; D006801:Humans; D011092:Polyethylene Glycols; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis
D012367:RNA, Viral; D014764:Viral Proteins; D011092:Polyethylene Glycols