[Localization of metallo-acceptor centers specifically binding divalent cations in myosin molecules].
The effects of DTNB and 4 M LiCl on ESR spectra saturation curves of myosin, spin-labelled at S1-SH groups in the presence of low concentrations of Mg2+ and Mn2+ ions have been studied. It has been shown that both DTNB and 4 M LiCl have taken off the influence of Mn2+ bound to high affinity metal binding sites of myosin on the character of saturation curves of spin-labelled protein. The results lead to a conclusion that high affinity metal binding sites of myosin are located in the LC2.light chains.
D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D002712:Chlorides; D004228:Dithionitrobenzoic Acid; D004578:Electron Spin Resonance Spectroscopy; D008094:Lithium; D018021:Lithium Chloride; D008274:Magnesium; D008345:Manganese; D009218:Myosins
D002712:Chlorides; D008345:Manganese; D004228:Dithionitrobenzoic Acid; D008094:Lithium; D009218:Myosins; D018021:Lithium Chloride; D008274:Magnesium
Issledovanie lokalizatsii metalloaktseptornykh tsentrov, spetsificheski sviazyvaiushchikh dvukhvalentnye kationy v molekule miozina.