[Effect of nuclease S1 on DNA of the highly oncogenic simian adenovirus SA7(C8)].
The effect at specific nuclease S1 on DNA and the complex viral DNA-terminal protein of the highly oncogenic simian adenovirus SA7(C8) was studied. It was shown that nuclease S1 did not digest the bound between DNA and terminal protein in the complex but residual amino acid(s) was cleaved out after digestion with pronase. The DNA obtained after nuclease S1 action could be ligated and its 5'-ends were phosphorylated by polynucleotide kinase.
D000256:Adenoviridae / Q000032:analysis*; D000261:Adenoviruses, Simian / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000032:analysis; D004720:Endonucleases; D011402:Pronase; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases; D011402:Pronase
Izuchenie deĭstviia nukleazy S1 na DNK vysokoonkogennogo adenovirusa obez'ian SA7(C8).