Water soluble receptors for human growth hormone from rabbit liver.
Soluble receptors that bind human growth hormone have been prepared by incubation of liver membranes form pregnant female rabbits in 1 mM Tris buffer (ph 7.5 or 9.0) at 4 degrees C. Up to 29% of the growth hormone binding sites could be solubilized within 48 hours. The kinetics of binding of human growth hormone to the soluble receptor, the hormonal specificity and the binding parameters calculated by Scatchard analysis (Ka 2.2 x 10(9) M-1, capacity 409 fmole/mg) were essentially unchanged compared with those for the parent membrane-associated (particulate) receptor. Gel filtration on Ultrogel AcA22 indicated that the major binding peak eluted at a molecular weight of 300,000 daltons. Specificity studies showed that the soluble binding sites had a moderately high affinity for ovine prolactin (Ka integral of 1 x 10(8) M-1), but negligible affinity for insulin. Although aqueous extraction gives a lower yield of binding sites for human growth hormone than detergent extraction, it nevertheless avoids some of the problems associated with use of detergents and should facilitate the subsequent purification of the receptor in a relatively unaltered state. It may also have applicability  for solubilization of other hormone receptor systems.
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008862:Microsomes, Liver / Q000032:analysis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011247:Pregnancy; D011388:Prolactin / Q000378:metabolism; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D011986:Receptors, Somatotropin; D012756:Sheep; D012995:Solubility; D014867:Water
D011956:Receptors, Cell Surface; D011986:Receptors, Somatotropin; D014867:Water; D011092:Polyethylene Glycols; D011388:Prolactin; D013006:Growth Hormone; D017830:Octoxynol