title: DNA fork displacement rates in human cells.
abstract: DNA fork displacement rates were measured in 20 human cell lines by a bromodeoxyuridine-313 nm photolysis technique. Cell lines included representatives of normal diploid, Fanconi's anemia, ataxia telangiectases, xeroderma pigmentosum, trisomy-21 and several transformed lines. The average value for all the cell lines was 0.53  0.08 mum/min. The average value for individual cell lines, however, displayed a 30% variation. Less than 10% of variation in the fork displacement rate appears to be due to the experimental technique; the remainder is probably due to true variation among the cell types and to culture conditions.
mesh_terms: D001260:Ataxia Telangiectasia / Q000378:metabolism; D001973:Bromodeoxyuridine; D002460:Cell Line; D002472:Cell Transformation, Viral; D004261:DNA Replication; D005199:Fanconi Anemia / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D010782:Photolysis; D013539:Simian virus 40; D014983:Xeroderma Pigmentosum / Q000378:metabolism
chemical_list: D001973:Bromodeoxyuridine