Immunoelectrophoretic evidence that the human syncytiotrophoblast transferrin receptor is identical to a major plasma membrane antigen present throughout pregnancy.
Crossed immunoelectrophoresis of solubilized syncytiotrophoblast microvillar proteins has been performed through intermediate gels, containing a monospecific antiserum to transferrin, into gels containing an antiserum raised to purified microvilli from term placentae. This technique resolved the microvillar transferrin-receptor complex into its component proteins. Crossed hydrophobic interaction immunoelectrophoresis confirmed that the transferrin receptor is an amphiphilic integral membrane protein. It is also a major antigen of the microvillar membrane and is present throughout pregnancy.
D000941:Antigens / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008871:Microvilli / Q000276:immunology; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D011990:Receptors, Transferrin; D014327:Trophoblasts / Q000032:analysis*
D000941:Antigens; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin