Potentiation by spiperone and other butyrophenones of fluid secretion by isolated salivary glands of ixodid ticks.
Isolated salivary glands from the ixodid tick, Amblyomma hebraeum Koch are stimulated to secrete fluid when exposed to dopamine (DA), the maximum response occurring at 10(-6) M. Spiperone, and a number of other butyrophenone derivatives, although lacking intrinsic activity, are able to potentiate the secretion elicited by supramaximal concentrations of DA; this potentiation by spiperone is evident at concentrations in the femtomolar range. Tranylcypromine, a potent, competitive inhibitor of monoamine oxidase (MAO) in tick salivary gland homogenates, has both intrinsic activity and potentiates DA-induced salivation. The fact that spiperone potentiates ergometrine-induced salivation that the prime mechanism of the butyrophenone effect is not by inhibiting catecholamine catabolism. The results also suggest that the receptor for DA and that for butyrophenones are distinct sites. Droperidol, benperidol and bromperidol, all potent neuroleptic drugs, failed (at 10(-9) M) to potentiate salivation. By contrast, R951, R27275 and R1187 (all at 10(-9) M) were very effective potentiators on the salivary gland system, despite the fact that they lack the basic structural requirements for neuroleptic activity. These results suggest that the butyrophenone site in tick salivary glands is different from butyrophenone binding sites in mammalian CNS.
D000818:Animals; D001665:Binding Sites; D002090:Butyrophenones / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D004357:Drug Synergism; D004874:Ergonovine / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D011954:Receptors, Dopamine / Q000187:drug effects; D012472:Salivation / Q000187:drug effects; D013134:Spiperone / Q000494:pharmacology*; D013987:Ticks / Q000502:physiology*
D002090:Butyrophenones; D008996:Monoamine Oxidase Inhibitors; D011954:Receptors, Dopamine; D013134:Spiperone; D000242:Cyclic AMP; D004298:Dopamine; D004874:Ergonovine