Hormonal control of phosphodiesterase activity in cultured rat Sertoli cells.
Phosphodiesterase activity was studied in cultures derived from 19-day-old rats and enriched with Sertoli cells. Pretreatment of such cultures with follicle-stimulating hormone or L-isoproterenol increased cAMP-phosphodiesterase activity 5.2 and 2.0 times, respectively. cGMP-phosphodiesterase activity was not affected. Similar effects were observed in freshly isolated cells. The stimulatory effect was enhanced by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine and was mimicked by cholera toxin and dbcAMP. Increased activity was observed after a latent period of 1 h. Stimulation was blocked by cycloheximide and actinomycin D. The enzyme had an apparent Km for cAMP of 1.4 micro M. Its activity was enhanced by Mg2+ but not by Ca2+. It is concluded that phosphodiesterases play an important role in the hormonal control of Sertoli-cell function and may contribute to the refractory state of these cells after stimulation with various agonists.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP; D006152:Cyclic GMP; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000201:enzymology*
D003609:Dactinomycin; D005640:Follicle Stimulating Hormone; D003513:Cycloheximide; D000242:Cyclic AMP; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D006152:Cyclic GMP; D008274:Magnesium; D007545:Isoproterenol; D002118:Calcium