title: Iron-sulphur cluster composition and redox properties of two ferredoxins from Desulfovibrio desulfuricans Norway strain.
abstract: Two ferredoxins from Desulfovibrio desulfuricans, Norway Strain, were investigated by EPR spectroscopy. Ferredoxin I appears to be a conventional [4Fe-4S]2+;1+ ferredoxin, with a midpoint reduction potential of -374 mV at pH 8. Ferredoxin II when reduced, at first showed a more complex spectrum, indicating an interaction between two [4Fe-4S] clusters, and probably, has two clusters per protein subunit. Upon reductive titration ferredoxin II changed to give a spectrum in which no intercluster interaction was seen. The midpoint potentials of the native and modified ferredoxin at pH 8 were estimated to be -500 and -440 mV, respectively.
mesh_terms: D003901:Desulfovibrio / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins; D007501:Iron; D010084:Oxidation-Reduction; D013455:Sulfur
chemical_list: D005288:Ferredoxins; D013455:Sulfur; D007501:Iron