title: DNA gyrase inhibitors block development of Bacillus subtilis bacteriophage SP01.
abstract: SP01 development was inhibited by nalidixic acid and novobiocin in the sensitive host Bacillus subtilis 168M. Inhibition by novobiocin was prevented by a Novr mutation in the cellular DNA gyrase gene. Nalidixic acid inhibition persisted in hosts carrying a Nalr gyrase, but could be overcome by phage mutation. We conclude that SP01 requires for its development subunit B of the host DNA gyrase, but replaces or modifies subunit A.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001435:Bacteriophages / Q000254:growth & development*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D009268:Nalidixic Acid / Q000494:pharmacology*; D009675:Novobiocin / Q000494:pharmacology*
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D009675:Novobiocin; D009268:Nalidixic Acid; D004250:DNA Topoisomerases, Type II