Characterization of the nuclear ribosomal DNA of Euglena gracilis.
A phage lambda recombinant library containing Euglena gracilis genomic DNA was screened for nuclear rDNA sequences. A recombinant phage was isolated that contained an 11.5-kb nuclear rDNA sequence. The 11.5-kb insert was mapped with restriction endonucleases and was shown to represent a complete rDNA repeat unit that carried the genes for the 19S, 25S, 5.8 S and 5 S cytoplasmic rRNAs. The 2000 rDNA repeat units per haploid genome are organized in the form of identical tandem repeats.
D001482:Base Composition; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005056:Euglena gracilis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D012335:RNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes