Effect of exogenous adenosine 3', 5'-cyclic monophosphate (cAMP) on the ultrastructure of neonatal rat hepatocytes in primary tissue culture. A stereological investigation.
Daily administration of exogenous cAMP to neonatal rat liver primary cultures caused a decrease of the total cellular volume, membrane space and SER coupled with a marked increase in the complement of RER, free ribosomes and polysomes and in the volume of nuclei after 3 days in hepatocytes; moreover the Golgi apparatus appeared to have undergone hypertrophy and the microtubules to have formed noticeable bundles. The present results support the view that the functioning of the RNA and protein synthesizing machinery of neonatal rat hepatocytes is stimulated by exogenous cAMP.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors
D008055:Lipids; D000242:Cyclic AMP