title: [Determination of protein secondary structure from circular dichroism spectra. III. Protein-derived base spectra of circular dichroism for antiparallel and parallel beta-structures].
abstract: Protein-derived basic CD spectra for alpha-helix, antiparallel and parallel beta-structures, beta-bends and irregular form of proteins have been determined from the experimental CD spectra of six (myoglobin, lysozyme, ribonuclease A, papain, lactate dehydrogenase, subtilisin BPN') or seven (glyceraldehyde-3-phosphate dehydrogenase added) reference proteins and the analysis of the X-ray data. The secondary structures of thirteen proteins (seven reference and six additional ones) have been analysed using the basic CD spectra thus obtained. The data obtained have been compared with the results of the X-ray data analysis. It is shown that the accuracy of determination of the beta-structure and beta-bends contents using our basic CD spectra is about 2-3 times better than using the basic spectra reported by Chang et al. (Analyt. Biochem. 91, 13-31, 1978).
mesh_terms: D002942:Circular Dichroism / Q000379:methods; D004720:Endonucleases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007770:L-Lactate Dehydrogenase; D009113:Muramidase; D009211:Myoglobin; D010206:Papain; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases; D013381:Subtilisins
chemical_list: D009211:Myoglobin; D007770:L-Lactate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D004720:Endonucleases; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic; D009113:Muramidase; D013381:Subtilisins; D010206:Papain
vernacular_title: opredelenie vtorichnoĭ struktury belkov iz spektrov krugovogo dikhroizma. III. Belkovye repernye spektry dlia antiparallel'noĭ i parallel'noĭ beta-struktur.