Cloned poliovirus complementary DNA is infectious in mammalian cells.
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D004279:DNA, Viral; D012367:RNA, Viral; D004262:DNA Restriction Enzymes