Characterization of the carbohydrate moiety in a purified allergen preparation from the mite Dermatophagoides farinae and its importance for allergenic activity as tested by rast-inhibition method.
The monosaccharide composition of a purified mite allergen preparation was mainly found to be: mannose, galactose, glucose, N-acetylglucosamine and N-acetylgalactosamine quantitated to 15% of dry material by means of methanolysis and GLC of trimethylsilylated  methyl glycosides. Protein content was found to be 65%, determined by the Lowry method. Methylation analysis and periodate oxidation indicated the main polysaccharide material to be built of 1,6-linked hexosamine, with side chains having mainly galactose end groups. Attempts to obtain separate carbohydrate fractions by ion exchange chromatography of the extract on CM-Sephadex and DEAE-Sephadex columns were not successful. The RAST-inhibition test showed a lower IgE binding capacity for periodate-treated mite preparation, where sugars containing vicinal diols have been oxidized, compared to an untreated mite preparation.
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight; D009005:Monosaccharides / Q000276:immunology*; D010504:Periodic Acid / Q000494:pharmacology; D011852:Radioallergosorbent Test
D000485:Allergens; D009005:Monosaccharides; D010504:Periodic Acid