Effect of alkylation on the secondary structure of DNA.
S1 nuclease hydrolysis and bezoylated naphthoylated DEAE-cellulose (BND-cellulose) chromatography have been used to demonstrate that alkylation of DNA by dimethyl sulfate at neutral pH leads to the production of partially denatured molecules under conditions where no significant depurination occurs. DNA was alkylated with increasing concentrations of the alkylating agent, and subjected to enzymatic degradation and binding to BND cellulose. An increasing degree of DNA hydrolysis and adherence to BND cellulose was seen. On hydroxyapatite chromatography the alkylated DNA still eluted at the position of double-stranded molecules suggesting the presence of partially denatured regions. The presence of salt had a preventive effect on such denaturation.
D000478:Alkylation; D002848:Chromatography, DEAE-Cellulose; D004247:DNA; D004720:Endonucleases; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013463:Sulfuric Acid Esters
D013463:Sulfuric Acid Esters; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; C007482:dimethyl sulfate