title: Metabolism and subsequent covalent binding of benzo[a]pyrene to macromolecules in gonads and liver of ripe english sole (Parophrys vetulus).
abstract: 1. Ripe English sole (Parophrys vetulus) force-fed [3H]benzo[a]pyrene, contained 1% of the dose in liver, 0.2% in ovary and 0.1% in testis, after 24 h. No significant change occurred in levels of radioactivity from 24 to 168 h. 2. Gonads and blood contained substantially larger proportions of unchanged benzo[a]pyrene (15-37% of tissue radioactivity) and organic solvent-soluble metabolites (6-35%) than did liver and bile. 3. T.l.c. revealed the presence of phenols, quinones, 7,8-dihydro-7, 8-dihydroxy- and 9,10-dihydro-9,10-dihydroxy-benzo[a]pyrene in liver and gonads. 4. A small proportion (less than 10%) of the radioactivity in liver and gonads was present as glucuronides and sulphates; bile contained a higher proportion (ca. 20%) of total radioactivity as glucuronides and sulphates. 5. Benzo[a]pyrene intermediates were covalently bound to liver proteins and DNA, and to a lesser extent to gonadal proteins (male and female fish) and gonadal DNA (confirmed for testis only).
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000008:administration & dosage / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006066:Gonads / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D001580:Benzopyrenes; D011506:Proteins; D001564:Benzo(a)pyrene; D004247:DNA