Influence of nonhistone chromatin protein HMG-1 on the enzymatic digestion of purified DNA.
The effect of chicken erythrocyte High Mobility Group protein 1 (HMG-1) on the enzymatic hydrolysis of purified double-stranded and single-stranded bacteriophage lambda DNA was studied. HMG-1 was found to inhibit the digestion of single- and double-stranded DNA by S1 nuclease and DNase I, respectively. HMG-I increased the rate of hydrolysis of double-stranded DNA by micrococcal nuclease, particularly at low HMG-1/DNA ratios, and had little effect on the hydrolysis of single-stranded DNA by micrococcal nucleases, even at high HMG-1 DNA ratios. We also present a semi-quantitative estimate that HMG-1 and HMG-2 occur in chromatin from rapidly dividing, cultured rat hepatoma cells at about 8 times the level that they occur in adult rat liver chromatin.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification; D004247:DNA; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D004912:Erythrocytes / Q000032:analysis; D006609:High Mobility Group Proteins; D006868:Hydrolysis; D007700:Kinetics; D008099:Liver / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D051381:Rats; D015719:Single-Strand Specific DNA and RNA Endonucleases
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006609:High Mobility Group Proteins; D004247:DNA; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D015719:Single-Strand Specific DNA and RNA Endonucleases