Methylcellulose media for plaque assay of murine leukemia virus.
When ecotropic murine leukemia virus was assayed by a methylcellulose-XC cell procedure, plaque titers showed less test-to-test variation, more uniform dose-response curves, and larger plaque sizes, as compared with results of the conventional liquid overlay-XC cell test system. This assay therefore seems to be reliable and useful for the titration of ecotropic murine leukemia virus.
D000818:Animals; D003470:Culture Media; D009052:Leukemia Virus, Murine / Q000254:growth & development* / Q000302:isolation & purification; D008747:Methylcellulose / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010948:Viral Plaque Assay / Q000379:methods*
D003470:Culture Media; D008747:Methylcellulose