The effect of bromodeoxyuridine and dibutyryl cyclic AMP on the induction of placental alkaline phosphate in human choriocarcinoma cells.
The effect of 5-bromo-2'-deoxyuridine (BrdUrd) and dibutyryl cyclic AMP (Bt2cAMP) on the expression of the placental isoenzyme of human alkaline phosphatase was examined in BeWo choriocarcinoma cells. By using a combination of specific immunoprecipitation and polyacrylamide-gel electrophoresis of cells labelled either metabolically with [35S]methionine or cell-surface-labelled with 125I, both BrdUrd (5 micrograms/ml) and 1 mM-Bt2cAMP were shown to result in the enhanced accumulation of a specific protein. This protein has immunochemical identity and co-electrophoreses with placental alkaline phosphatase in two-dimensional gels. These results clearly demonstrate that the induction of placental alkaline phosphatase activity in choriocarcinoma cells treated with these agents is a consequence of the accumulation of specific enzyme protein rather than of altered catalytic activity.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D002822:Choriocarcinoma / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000201:enzymology*
D007527:Isoenzymes; D003994:Bucladesine; D000469:Alkaline Phosphatase; D001973:Bromodeoxyuridine