Removal of superoxide dismutase activity from cytochrome C.
Commercially available cytochrome c contains sufficient superoxide dismutase activity to reduce its sensitivity in superoxide anion detection. A single passage through a column of Sephadex G-50 removes the superoxide dismutase, and appreciably increased the ability to cytochrome c to detect superoxide.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D003574:Cytochrome c Group / Q000302:isolation & purification*; D006736:Horses; D009206:Myocardium; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000302:isolation & purification*; D013481:Superoxides / Q000032:analysis
D003574:Cytochrome c Group; D013481:Superoxides; D013482:Superoxide Dismutase