Exchange transfusion with Fluosol 43: in vivo assessment of cerebral cytochrome c oxidase redox state.
Rats were serially exchange transfused to hematocrits less than 2% (50-cc exchange) with either Krebs-Henseleit-albumin (KHA) solution or Fluosol-43 (F-43). Reflectance spectrophotometry was employed to monitor cerebral cytochrome c oxidase redox states. Determination of mean arterial pressure (MAP), arterial and venous oxygen content difference (A-VO2), and cerebral cortical ATP and lactate concentrations were also made. As hemodilution progressed, neither solution provided normal quantities of oxygen as evaluated by the cytochrome c oxidase redox state and a decrease in A-VO2 difference, although F-43 was significantly (p less than 0.01) better than KHA in terms of cytochrome c oxidase redox state as well as maintenance of MAP. There was no significant difference between the two test solutions in terms of cortical ATP and lactate.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D001802:Blood Substitutes / Q000627:therapeutic use*; D001921:Brain / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005078:Exchange Transfusion, Whole Blood; D005466:Fluorocarbons / Q000627:therapeutic use*; D007773:Lactates / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010100:Oxygen / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains
D001802:Blood Substitutes; D003574:Cytochrome c Group; D005466:Fluorocarbons; D007773:Lactates; C008807:perfluorotributylamine; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D010100:Oxygen