title: Immunofluorescent staining of nuclear antigen in lymphoid cells transformed by Herpesvirus papio (HVP).
abstract: An improved fixation method for antigen detection in lymphoblastoid cells is described. Herpesvirus papio nuclear antigen (HUPNA) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (ACIF). Antibody to HUPNA was detected in many human sera containing antibodies to Epstein-Barr virus capsid and nuclear antigen (EBNA). Rheumatoid arthritis sera showed a high incidence of both anti-EBNA and anti-HUPNA antibodies.
mesh_terms: D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral / Q000096:biosynthesis; D000941:Antigens; D002467:Cell Nucleus / Q000276:immunology*; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation
chemical_list: D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral; D000941:Antigens; D005404:Fixatives