title: Mutant carrying deletions in the two simian virus 40 early genes.
abstract: We isolated a simian virus 40 mutant, dl2194, which carried deletions in both early genes. One deletion removed 234 base pairs in the 54/59 region within the small-t-antigen coding sequence and the large-T-antigen gene intron. The second deletion removed 57 base pairs at the C terminus of the large-T-antigen coding sequence (0.20 map unit). dl2194 was a viable mutant, it carried a normal helper function for adenovirus growth on monkey cells, and it displayed the transformation properties of a small-t-antigen-negative single mutant. Therefore, none of the known large-T-antigen functions seemed to be altered by the C-terminal deletion.
mesh_terms: D000260:Adenoviruses, Human / Q000254:growth & development; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D000956:Antigens, Viral / Q000235:genetics*; D000957:Antigens, Viral, Tumor; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral; D005814:Genes, Viral; D000882:Haplorhini; D006378:Helper Viruses / Q000502:physiology*; D006801:Humans; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology
chemical_list: D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral