Prostacyclin prolongs viability of washed human platelets.
The functional viability of stored human platelets, washed in the presence and absence of prostacyclin, was examined over a 96 h period. Platelet counts, aggregation responses and cyclic AMP levels were monitored as well as the spontaneous generation of thromboxane B2 and the liberation of labelled oleate from cellular phosphatides. In suspensions prepared without prostacyclin in the medium, platelet counts declined rapidly as did the sensitivity to aggregating agents. In addition, substantial amounts of thromboxane B2 were generated during preparation and storage and oleate liberation occurred at a rapid rate. In contrast, in prostacyclin-washed platelets, aggregation was maintained throughout the study period and there was little generation of thromboxane B2. Moreover, only a gradual decrease in platelet count and a slow increase in the rate of oleate liberation was observed when compared with controls. However, cyclic AMP levels rapidly declined when platelets were resuspended in prostacyclin-free medium.
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D001800:Blood Specimen Collection; D002470:Cell Survival / Q000187:drug effects; D000242:Cyclic AMP / Q000097:blood; D011464:Epoprostenol / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D019301:Oleic Acid; D009829:Oleic Acids / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000097:blood
D009829:Oleic Acids; D011453:Prostaglandins; D019301:Oleic Acid; D013929:Thromboxane B2; D011464:Epoprostenol; D000242:Cyclic AMP