title: [A new human hepatoma cell line: establishment and characterization].
abstract: We have grown a human hepatoma cell line, designated as HA22T/VGH, from a 52-yr-old male hepatoma patient since July 1, 1980. This cell line has been subcultured more than 100 passages. The chromosome analysis of HA22T/VGH indicated that the chromosome numbers varied from 70 to 146, with the mode of 73. Methylcellulose soft agar assay showed that approximately 40% of the HA22T/VGH cells formed colonies. The HA22T/VGH produced tumors in nude mice. Histopathological studies of the tumor revealed the arrangement of hepatoma. Detected by the complement fixation method HA22T/VGH cells secreted ceruloplasmin, Factor B, C3, C4, Gc-globulin and alpha 1-acid-glycoprotein. These cells contained the liver associated enzymes: alanine amino transferase, tyrosine amino transferase and gamma-glutamyl transferase. HBsAg and alpha-fetoprotein were not detectable in the HA22T/VGH culture media or cell lysates by the radioimmunoassay.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000032:analysis / Q000473:pathology*; D002460:Cell Line; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis / Q000473:pathology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009368:Neoplasm Transplantation
chemical_list: D001798:Blood Proteins