Expression of transforming region of Moloney murine sarcoma virus in Escherichia coli as a fusion protein with small tumor antigen of polyoma virus.
Bacterial expression of the transforming region of Moloney murine sarcoma virus, designated mos, was obtained as a fusion protein with a portion of the small tumor antigen of polyoma virus. This was accomplished by fusing the entire mos open reading frame, encoding a 41,000-dalton protein, with a plasmid that expresses a beta-galactosidase-polyoma fusion protein under lac operon control. The resulting plasmid directed synthesis of the predicted polyoma antigen-sarcoma virus fusion protein of 59,000 daltons. This protein was immunoprecipitated by an anti-polyoma tumor antigen antiserum that recognized polyoma determinants at the NH2 terminus of the hybrid protein. This protein was also immunoprecipitated by an antiserum directed against a synthetic peptide containing the 12 COOH-terminal amino acids encoded by the mos open reading frame. This work confirms the existence of a long open reading frame in the mos gene and resolves a discrepancy between different nucleotide sequences for its COOH-terminal coding region.
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics; D000956:Antigens, Viral / Q000235:genetics; D000957:Antigens, Viral, Tumor; D002472:Cell Transformation, Viral; D004274:DNA, Recombinant; D003673:Defective Viruses / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D007763:Lac Operon; D051379:Mice; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000235:genetics*; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics; D014176:Protein Biosynthesis; D009053:Sarcoma Viruses, Murine / Q000235:genetics*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant