Construction of a dominant selective marker useful for gene transfer studies in animal cells.
Biochemically transformed clones of murine, simian and human cells were obtained after transfection with a new dominant selective marker. This marker is a hybrid gene which was constructed with a bacterial neomycin-kanamycin resistance gene coding region and the transcription signals of the herpes simplex virus thymidine kinase gene.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D005796:Genes; D005799:Genes, Dominant; D005818:Genetic Engineering; D007739:L Cells / Q000502:physiology; D051379:Mice; D010957:Plasmids; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D013937:Thymidine Kinase