title: A novel endonuclease specified by bacteriophage lambda. Purification and properties of the enzyme.
abstract: A DNA endonuclease whose expression is under the control of the b region of bacteriophage lambda has been partially purified from an induced lambda lysogen. In a reaction that requires single-stranded DNA, ATP, and Mg2+, the lambda-induced endonuclease makes one double strand break in pBR322 and other covalently closed circular DNA molecules, converting these substrates into unit-length linear forms. The double strand break in pBR322 DNA occurs at one of several preferred sites. Linear DNA appears not to be a good substrate for the enzyme.
mesh_terms: D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004278:DNA, Superhelical / Q000235:genetics; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005814:Genes, Viral; D007700:Kinetics; D008854:Microscopy, Electron; D008970:Molecular Weight; D013379:Substrate Specificity
chemical_list: D004278:DNA, Superhelical; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I