Phosphatidylinositol-hydrolysing enzymes in blowfly salivary glands.
The salivary glands of adult blowflies (Calliphora erythrocephala) contain enzymes that hydrolyse phosphatidylinositol, predominantly by a Ca2+-independent deacylation, though a Ca2+-dependent phosphodiesterase (phospholipase C) activity could be detected. The deacylating enzymes could also hydrolyse phosphatidylcholine and phosphatidylethanolamine, and were secreted in the saliva. Homogenization of salivary glands prelabelled with [3H]inositol resulted in a rapid deacylation of the endogenous 3H-labelled phosphatidylinositol; this hydrolysis was unaffected by addition of 5-hydroxytryptamine to the homogenate.
D000818:Animals; D004175:Diptera / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism; D008245:Lysophospholipase / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012469:Salivary Glands / Q000187:drug effects / Q000201:enzymology; D012701:Serotonin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D010716:Phosphatidylinositols; D012701:Serotonin; D007294:Inositol; D010741:Phospholipases A; D008245:Lysophospholipase; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase