title: The sequence of the nucleoprotein gene of human influenza A virus, strain A/NT/60/68.
abstract: The nucleotide sequence of the nucleoprotein gene of influenza A/NT/60/68 was established after using improved cloning methods to obtain full length cDNA clones in pBr322. The gene is 1565 residues long and codes for a basic protein of 498 amino acids. There are only 30 amino acid differences between it and the homologous sequence in A/PR/8/35, all occurring as point mutations. Assuming a common lineage, the evolutionary rate of divergence of the two strains is 0.18% amino acid per year. This confirms there is a slow but significant rate of evolution.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D003852:Deoxyribonucleoproteins; D005814:Genes, Viral; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D009154:Mutation; D009698:Nucleoproteins; D009975:Orthomyxoviridae / Q000235:genetics; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics
chemical_list: D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D014764:Viral Proteins; D004247:DNA; D004262:DNA Restriction Enzymes