Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates commercial preparations of various nucleotides and disposable glass test tubes, has been identified as Al3+. In the presence of 10 mM Mg2+ and 5 mM F-, AlCl3 causes activation of G/F with an apparent activation constant of approximately 1-5 muM. The requirement for Al3+ is highly specific; of 28 other metals tested, only Be2+ promoted activation of G/F by F-.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D005459:Fluorides / Q000494:pharmacology*; D019204:GTP-Binding Proteins; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism; D013329:Structure-Activity Relationship
D001798:Blood Proteins; D011956:Receptors, Cell Surface; D000255:Adenosine Triphosphate; D000535:Aluminum; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D005459:Fluorides