Immunoglobulin secretion by human B lymphocytes exposed to herpes simplex type I antigen.
Herpes simplex type I (HSV I) antigen was studied for its capacity to induce immunoglobulin(Ig)-secreting cells in human peripheral-blood lymphocytes. Ig-secreting cells were detected using an indirect hemolytic plaque assay. These results were compared to those obtained by using pokeweed mitogen (T-dependent) and Epstein-Barr virus (T-independent) to induce Ig-secreting cells. The HSV I induction of Ig synthesis was T-dependent, similar to the pokeweed-mitogen system. Peripheral-blood mononuclear cells depleted of T cells did not produce immunoglobulin. In contrast, the same T-depleted cell populations stimulated with Epstein-Barr virus (another member of the Herpes group) produced Ig. Lymphocytes in the HSV-I-stimulated cultures were found to secrete immunoglobulins of the IgA, IgG, and IgM classes. It was the lymphocytes from seropositive individuals that made Ig after HSV I stimulation. Such responses suggest that this system may be antigen-specific. This T-dependent virally induced Ig synthesis system may be useful in studying immunodeficient and immunocompromised individuals.
D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008211:Lymphocyte Cooperation; D011043:Pokeweed Mitogens / Q000494:pharmacology; D018139:Simplexvirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000956:Antigens, Viral; D007136:Immunoglobulins; D011043:Pokeweed Mitogens