A transforming gene present in human sarcoma cell lines.
Morphological transformation of NIH/3T3 cells by transfection with DNA has been used to identify transforming sequences in human tumours. Transforming activity has been reported for DNAs isolated from bladder, mammary, colon and lung carcinomas, neuroblastoma, lymphoid and myeloid tumours. Each of these tissues seems to contain different transforming sequences except for the colon and lung tumours where the same sequence seems to be involved. We now report that in two different human sarcoma cell lines, a fibrosarcoma and an embryonal rhabdomyosarcoma, the DNAs have transforming activity. The transforming gene is the same in both sarcomas but differs from the activated sequences detected in other tumours. We have also found that the transforming gene has no detectable homology to eight retrovirus oncogenes tested.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004273:DNA, Neoplasm; D005354:Fibrosarcoma / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics; D012208:Rhabdomyosarcoma / Q000235:genetics*; D014162:Transfection
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes