Isolation and characterisation of two strains of Herpesvirus hominis type 1 from fallopian tubes.
Herpesvirus hominis type 1 (HSV-1) was isolated from fallopian-tube biopsies of two women who were using an intra-uterine contraceptive device; the strains were isolated on different dates and at different hospitals. Both the isolates were shown to be HSV-1 by restriction-enzyme analysis, polypeptide profiles and indirect immuno fluorescence; the two isolates could not be distinguished by these tests, but the tests distinguished them from HSV-1 strain 71-15 and HSV-2 strain 333. The viruses were again isolated from samples of fallopian-tube tissue stored in liquid nitrogen, and their polypeptide and restriction-enzyme profiles were the same as those in the initial isolates. Virus was not isolated from fallopian-tube samples obtained from other IUD patients undergoing surgery at the same two hospitals at the same time.
D001706:Biopsy; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005187:Fallopian Tubes / Q000382:microbiology*; D005260:Female; D006561:Herpes Simplex / Q000209:etiology; D006801:Humans; D007434:Intrauterine Devices / Q000009:adverse effects; D018139:Simplexvirus / Q000302:isolation & purification*
D004247:DNA; D004262:DNA Restriction Enzymes