title: Intestinal phospholipase A and triglyceride lipase: localization and effect of fasting.
abstract: We investigated the distribution of phospholipase A and triglyceride lipase in the rat small intestine and the effects of heparin and hormones on enzyme release. Phospholipase A activity was 10 times higher in the ileum than in the jejunum; triglyceride lipase activity was threefold higher in the jejunum than in the ileum. Activities of both enzymes were much greater in villus than in crypt cells. The specific activity of phospholipase A was highest in microsomes and least in cytosol. The crude nuclei and brush-border fraction contained 40.5% of total phospholipase A activity; mitochondria contained 33.8%; and microsomes, 17.4%. Phospholipase A activity increased significantly in the distal intestinal mucosa in fasted rats compared with controls. Heparin did not increase the release of phospholipase A by isolated intestinal cells or perfused intestinal vasculature. Thus, the small intestine probably does not contribute significantly to the phospholipase A activity of postheparin plasma. Hormones and cAMP, which inhibit the secretion of phospholipase A and triglyceride lipase from isolated hepatocytes, had no effect on the release of either enzyme from intestinal cells.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005215:Fasting; D005934:Glucagon / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D008049:Lipase / Q000378:metabolism*; D008297:Male; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D051381:Rats
chemical_list: D003994:Bucladesine; D006493:Heparin; D005934:Glucagon; D010740:Phospholipases; D008049:Lipase; D010741:Phospholipases A; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase; D004837:Epinephrine