In vitro transcription of two human rotaviruses.
The RNA polymerase activities of a cultivatable (Wa) and a noncultivatable (DS-1) strain of human rotavirus were studied. Under optimal conditions, transcription of all of their RNA segments occurred, as evidenced by the hybridization of labeled transcripts to genomic RNA. Cross-hybridization between the two viruses showed that none of their 11 genes were completely homologous. The transcription products could be translated in vitro, yielding proteins with an electrophoretic pattern resembling that obtained with proteins labeled in vivo during infection with the Wa virus.
D002474:Cell-Free System; D002648:Child; D006801:Humans; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D012401:Rotavirus / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase