Nonenzymatic separation of myocardial cell nuclei from whole heart tissue.
A simple and rapid nonenzymatic method has been developed to isolate myocardial cell nuclei from whole heart tissue. This method consists of a controlled disruption of cells followed by isopycnic gradient centrifugation. We have reviewed and compared our method to others more lengthy and laborious. By using a number of criteria, such as morphometric measurements, chemical composition, functional studies, specific nuclear protein markers, and mathematical analysis, we show that the nonenzymatic digestion method provides a most useful technique for the study of the biochemistry of the myocardial cell nucleus.
D000375:Aging; D000818:Animals; D001011:Aorta / Q000502:physiology; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D002500:Centrifugation, Isopycnic; D003250:Constriction; D004247:DNA / Q000096:biosynthesis; D004492:Edetic Acid; D006657:Histones / Q000378:metabolism; D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase; D009206:Myocardium / Q000648:ultrastructure*; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014357:Trypsin
D006657:Histones; D010761:Phosphorus Radioisotopes; D004247:DNA; D004492:Edetic Acid; D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D003012:Microbial Collagenase; D013936:Thymidine