DNA-polymerase induced by Herpesvirus papio (HVP) in cells of lymphoblastoid cultures derived from lymphomatous baboons. Report V.
A new DNA-polymerase was found in the cells of suspension lymphoblastoid cultures which produce lymphotropic baboon herpesvirus (HVP). This enzyme was isolated in a partially purified form. Some of its properties vary from those of other cellular DNA-polymerases. HVP-induced DNA-polymerase has a molecule weight of 160,000 and sedimentation coefficient of about 8 S. The enzyme is resistant to high salt concentration and N-ethylmaleimide, but it is very sensitive to phosphonoacetate. It effectively copies "activated" DNA and synthetic deoxyribohomopolymers. Attempts to reveal the DNA-polymerase activity in HVP virions were unsuccessful.
D000818:Animals; D002478:Cells, Cultured; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000302:isolation & purification; D004790:Enzyme Induction; D006564:Herpesviridae / Q000502:physiology*; D006566:Herpesviridae Infections / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008223:Lymphoma / Q000201:enzymology; D010215:Papio
D004259:DNA-Directed DNA Polymerase