title: Analysis of the genomes of bluetongue virus serotype 10 vaccines and a recent BTV-10 isolate from Washington.
abstract: The 10 double-stranded RNA gene segments of 2 vaccinal strains of bluetongue virus (BTV) serotype 10 that are used in the United States (BTV CA8 California and BT-8 Colorado), and a BTV-10 isolate recently obtained from infected sheep in Washington (state) were characterized by oligonucleotide fingerprint analyses. It was determined that although the 2 BTV-10 vaccinal strains are genotypically distinct, they are closely related both to each other and to the United States prototype BTV-10 virus. The BTV-10 field isolate appears to be a naturally occurring reassortment virus with genome segments derived from both United States prototype BTV-10 and BTV-11 viruses. However, one RNA segment of the isolate was totally unlike the corresponding segments of United States prototype BTV-10, -11, -13 and -17 viruses.
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000382:microbiology*; D001820:Bluetongue virus / Q000032:analysis* / Q000145:classification / Q000302:isolation & purification; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000032:analysis*; D012756:Sheep; D014765:Viral Vaccines; D014861:Washington
chemical_list: D009841:Oligonucleotides; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014765:Viral Vaccines