[Nuclease S1 for DNA molecular weight determination in multiplasmid Escherichia coli strains].
DNase treatment used to convert supercoiled DNAs to the open form is suitable for molecular weight determination by electron microscopy or agarose gel electrophoresis. The comparative experiments showed that S1-nuclease has advantages over DNase for molecular weight determination in preparations containing many species of plasmids of significantly differing sizes, isolated from multiplasmid E. coli strains.
D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000032:analysis*; D008854:Microscopy, Electron; D008970:Molecular Weight; D010957:Plasmids; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013045:Species Specificity
D004269:DNA, Bacterial; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases
Primenenie nukleazy S1 dlia opredeleniia molekuliarnoĭ massy DNK v mul'tiplazmidnykh shtammakh Escherhicia coli.