Differential effect of nucleoside analog triphosphates on ribonucleotide reductases from uninfected and herpes simplex virus-infected HeLa cells.
Effects of the triphosphates of eight pyrimidine nucleoside analogs (5-substituted, 2'-fluoroara-, and acyclonucleosides) and acycloguanosine were examined on the ribonucleotide reductases prepared from uninfected and herpes simplex virus (types 1 and 2)-infected HeLa cells. Of the analogs tested, E-5-propenyl- and E-5-(2-bromovinyl)-dUTP were more potent inhibitors than dTTP of the enzymes from virus-infected cells, whereas only the former compound showed this effect on the uninfected HeLa enzyme.
D000212:Acyclovir; D003856:Deoxyuracil Nucleotides / Q000494:pharmacology; D006147:Guanine / Q000031:analogs & derivatives / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D009711:Nucleotides / Q000494:pharmacology*; D012264:Ribonucleotide Reductases / Q000037:antagonists & inhibitors*; D018139:Simplexvirus / Q000201:enzymology* / Q000254:growth & development; D013329:Structure-Activity Relationship; D013942:Thymine Nucleotides / Q000494:pharmacology
D003856:Deoxyuracil Nucleotides; D009711:Nucleotides; D013942:Thymine Nucleotides; D006147:Guanine; C033642:5-(2-bromovinyl)-2'-deoxyuridine-5'-triphosphate; C035128:5-propenyl-dUTP; D012264:Ribonucleotide Reductases; C024157:thymidine 5'-triphosphate; D000212:Acyclovir