[Isolation and properties of an alpha-galactosidase preparation from Cephalosporium sp. 237].
Different methods for preparing alpha-galactosidase from the culture fluid of the micromycete Cephalosporium sp. 237 were tested. They included precipitation with ethanol, isopropanol, and acetone. Precipitation with two volumes of acetone gave the best results with respect to specific activity (12 units) and yield of the enzyme (78%). Properties of alpha-galactosidase (optimum pH at 5.5, temperature optimum at 40 degrees C. thermolability, etc), when different substrates were used, were examined.
D000164:Acremonium / Q000201:enzymology*; D005696:Galactosidases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008722:Methods; D013696:Temperature; D000519:alpha-Galactosidase / Q000302:isolation & purification* / Q000378:metabolism
D005696:Galactosidases; D000519:alpha-Galactosidase
Poluchenie i svoĭstva preparata al'fa-galaktozidazy iz Cephalosporium sp. 237.