The nature of the DNA associated with incomplete particles of adenovirus type 2.
The nature of the DNA in incomplete particles (IP) synthesized by adenovirus type 2 and the ts4 mutant which accumulates such particles were analysed by agarose gel electrophoresis, restriction endonuclease cleavage and blot hybridization techniques. IP DNA consisted of a heterogeneous population of subgenomic-size DNA (IPSD1) and smaller molecules ranging from about 1000 base pairs to 200 base pairs (IPSD2). IPSD1 from ts4 was more heterogeneous than that from wild-type (wt), but both contained sequences from all parts of the viral genome. IPSD2 contained heterogeneous cellular sequences and viral sequences from the left 4.4% of the genome. An endonuclease activity associated with IP and virions was capable of digesting viral or cellular DNA to IPSD2-like fragments suggesting a possible origin for these molecules.
D000260:Adenoviruses, Human / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D003673:Defective Viruses / Q000201:enzymology / Q000235:genetics*; D003851:Deoxyribonucleases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism; D005814:Genes, Viral; D012367:RNA, Viral
D004279:DNA, Viral; D012367:RNA, Viral; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes