The relation between polyoma T-antigen and increased 5S RNA synthesis in cell-free extracts from polyoma-infected mouse kidney cell cultures.
In polyoma-infected mouse kidney cell cultures 5S RNA synthesis began to increase around 16 h, i.e. 7-9 h after the onset of polyoma T-antigen synthesis. The rate of polyoma-induced 5S RNA synthesis reached a maximum plateau around 25 h when it was 1.8-2.0 times higher than in mock-infected parallel cultures. Stimulation of 5S RNA synthesis in vivo thus coincided in time with the increase in total cellular RNA and protein. Cell-free extracts (S100) prepared at 15 h from mock-(S100-M) or polyoma-infected (S100-Py) mouse kidney cell cultures were indistinguishable with respect to protein concentration and 5S RNA synthesis, using a cloned somatic Xenopus borealis 5S gene as template. S100-Py extracted 25 h after infection contained 30% more protein and synthesized 1.5-2.0 times more 5S RNA than S100-M. Complete removal of the polyoma T-antigens from S100-Py by 3 cycles of immunoprecipitation with hamster anti-T serum remained without effect on stimulated 5S RNA synthesis. However, a linear relationship between 5S RNA synthesis and protein concentration of S100-M and S100-Py was observed.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D000957:Antigens, Viral, Tumor; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D011120:Polyomavirus / Q000276:immunology*; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D012335:RNA, Ribosomal