Phylogeny and ontogeny of the phosphoglycerate mutases.--V. Inactivation of phosphoglycerate mutase isozymes by histidine-specific reagents.
1. The three isozymes of glycerate-2,3-P2 dependent phosphoglycerate mutase present in tissues of mammals and reptiles were inactivated by both treatment with diethylpyrocarbonate and photooxidation with rose bengal. 2. Inactivation of type M isozyme purified from rabbit muscle was complete when two histidine residues per enzyme subunit were carboethoxylated. Hydroxylamine removed the carboethoxy groups, with partial recovery of the enzymatic activity. The cofactor protected the enzyme against inactivation. 3. The inactivation of rabbit muscle phosphoglycerate mutase by photooxidation with methylene blue and rose bengal was sharply pH dependent. The pH profile of enzyme inactivation followed the titration curve of histidine, suggesting that this amino acid was critical for enzyme activity. Glycerate-2,3-P2 did not protect phosphoglycerate mutase against photoinactivation.
D000818:Animals; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D005561:Formates / Q000494:pharmacology*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors*; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D011817:Rabbits; D051381:Rats; D012395:Rose Bengal / Q000494:pharmacology; D013045:Species Specificity; D014020:Tissue Extracts / Q000378:metabolism; D014426:Turtles
D005561:Formates; D007527:Isoenzymes; D014020:Tissue Extracts; D012395:Rose Bengal; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase; D004047:Diethyl Pyrocarbonate; D008751:Methylene Blue