Simple assay method for susceptibility of human lymphocytes to Epstein-Barr virus infection.
On the basis of the sequential early events induced by Epstein-Barr virus (EBV), the infectivity of human lymphocytes to EBV was quantified as a plateau value of the maximum nuclear antigen (EBNA) synthesis preceding cellular DNA synthesis. The frequency of EBV-infectible cells was an average of 7.6% of unfractionated umbilical cord lymphocytes and was 4.9% in the counterparts from human adults. In the B-cell-enriched populations, the average plateau values were 29.2% in umbilical cord and 15.5% in adult samples. This method is simple and applicable for quantitative assay of the sensitivity to EBV infection of a given lymphocyte preparation.
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000382:microbiology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D019309:Epstein-Barr Virus Nuclear Antigens; D005312:Fetal Blood; D004854:Herpesvirus 4, Human / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D008828:Microbiological Techniques
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D004247:DNA