Preformed magnesium hydroxide precipitate for second-step concentration of enteroviruses from drinking and surface waters.
A method is described for the second-step concentration of viruses from large volumes of drinking and surface waters. Seeded viruses present in the first eluate, performed with 50 mM glycine buffer, pH 11.5, were adsorbed on a preformed magnesium hydroxide precipitate. After low-speed centrifugation they were desorbed and adjusted to pH 7 with McIlvaine citrate-phosphate buffer. In these experimental conditions 90% of the viruses present in the 300-mL first eluate were reconcentrated in a final volume of 40 mL. The recovery efficiency was independent of either virus concentration or water quality.
D011232:Chemical Precipitation; D004770:Enterovirus / Q000302:isolation & purification*; D005618:Fresh Water; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D008276:Magnesium Hydroxide; D008722:Methods; D017955:Poliovirus / Q000302:isolation & purification; D014871:Water Microbiology; D014881:Water Supply
D008274:Magnesium; D008276:Magnesium Hydroxide