Enzymatic impairment induced by biological aldehydes in intact rat liver cells.
The addition of lipid peroxidation end-products, 4-hydroxypentenal (HPE), 4-hydroxynonenal (HNE) or hexanal (HEX) to the incubation medium of rat hepatocytes caused a significant decrease of cytochrome P-450 content and inactivation of total cell glucose-6-phosphatase. In particular, the two hydroxyalkenals exerted their inhibitory action at micromolar concentration. These results on intact cells further support a possible damaging effect of aldehydes in pathological conditions in which a stimulation of lipid peroxidation occurs.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000447:Aldehydes; D008054:Lipid Peroxides; D003577:Cytochrome P-450 Enzyme System; C010463:n-hexanal; D005952:Glucose-6-Phosphatase; C027576:4-hydroxy-2-nonenal