[Cell functions of cultured human choriocarcinoma following the administration of methotrexate (author's transl)].
Treatment of choriocarcinoma should be eradicated of trophoblastic cells which undergo destruction in both of primary and metastatic sites and Methotrexate (MTX) has a most effective value. While MTX is being administration to the patients, there is no determination of clear relationship between proliferation of trophoblastic cells and production of human chorionic gonadotropin (hCG). In this report, we attempted to clarify the changes of the dividing compartments in cell cycle of human choriocarcinoma in vitro in the administration of MTX (concentration in 5 x 10(-6) M and 5 x 10(-7) M) to the experimental cells. Cell samples were stained by propidium iodide (PI) and fluorescein isothiocyanate (FITC), then were measured nucleic acid and protein content by means of flow microfluorometry (FMF). MTX was remarkably increased the intensity of FITC associated with protein production, in dose that is not only killing effect to the cells but inhibition of DNA synthesis. Moreover, the most difference in both subjects were as follows; Ever when MTX in low concentration was removed after 48 hours, 50% of experimental cells recovered to the level of controls in contrast to the persistence of remarkable inhibition of DNA synthesis in high concentration.
D002453:Cell Cycle; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000648:ultrastructure*; D006063:Chorionic Gonadotropin / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D011247:Pregnancy; D013861:Thiocyanates; D014328:Trophoblastic Neoplasms / Q000648:ultrastructure; D014594:Uterine Neoplasms / Q000648:ultrastructure*
D006063:Chorionic Gonadotropin; D004273:DNA, Neoplasm; D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; D008727:Methotrexate