title: Adenoassociated virus has a unique chromatin structure.
abstract: The organization of intranuclear adenoassociated virus DNA (AAV) was examined following micrococcal nuclease digestion of nuclei prepared from cells coinfected with AAV type 2 (AAV-2) and adenovirus type 2 (Ad2). Blot-hybridization analysis of the DNA with AAV-2, Ad2, and cellular DNA probes revealed that AAV-2 chromatin has a unique structure, which upon nuclease digestion gives rise to a smear of oligomeric DNA fragments from 600-2200 base pairs in length with only a very faint band about 160 base pairs and no discrete multimers. This structure was similar to, but distinguishable from, Ad2 chromatin and completely unrelated to eukaryotic chromatin.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008836:Micrococcal Nuclease / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology
chemical_list: D002843:Chromatin; D004279:DNA, Viral; D046911:Macromolecular Substances; D010664:Phenylmethylsulfonyl Fluoride; D004247:DNA; D008836:Micrococcal Nuclease