Control of glycosaminoglycan metabolism by ACTH in bovine adrenocortical cells in primary culture.
Bovine adrenocortical cells in primary culture actively incorporated [35S]sodium sulphate and [3H]glucosamine into glycosaminoglycans (GAGs). Most of the synthesized GAGs were found associated with the pericellular material and secreted in the culture medium, while less than 15% remained associated with the cell pellet. In all these fractions the major product was identified by its molecular properties and selective degradation procedures as a heparan sulphate structure. Exposure of the cells to ACTH induces a shift of the synthesized GAGs from the cellular to the pericellular compartment, an increase in the heparan sulphate labelling and the occurrence of an additional product characterized as a hyaluronate. These data suggest that GAG metabolism of the adrenocortical cell may be under hormonal control and open the possibility of a modulation of cell activity through modifications of its GAG components, especially those involved in the cell matrix architecture.
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006497:Heparan Sulfate / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
D000324:Adrenocorticotropic Hormone; D005944:Glucosamine; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D013431:Sulfates; C012036:sodium sulfate