title: Probing cII and himA action at the integrase promoter pi of bacteriophage lambda.
abstract: Plasmids were constructed to supply cII-coded protein for activation of the phage promoter pI. Using a fusion which expresses lacZ from pI. We can accurately follow activation of pI without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of pI is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type pI. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for cII-stimulated transcription at pI.
mesh_terms: D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005838:Genotype; D019426:Integrases; D009876:Operon; D010957:Plasmids; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D019426:Integrases; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase; D004265:DNA Helicases