Flow cytometric analysis of DNA content of mouse liver cells following in vivo infection by human adenovirus type 5.
Human adenovirus type 5 caused acute hepatocellular damage when injected intravenously into C57B1/6 mice. Early protein (P) and late virus protein (V) antigen staining by immunofluorescence was located principally, if not exclusively, in hepatocytes. Autoradiography following incorporation of tritiated thymidine into Ad5-infected liver cells in vivo indicated that DNA synthesis was initiated in hepatocytes. Flow cytometry showed that cells with a 4n DNA content prior to infection were highly susceptible to infection, with 75% being lost from the liver cell population by 5 days post-infection. This observation was correlated with the observed pathology during the course of the infection.
D000257:Adenoviridae Infections / Q000378:metabolism* / Q000473:pathology; D000260:Adenoviruses, Human / Q000254:growth & development; D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D005434:Flow Cytometry; D008099:Liver / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins; D004247:DNA