The inflammatory effects of hydroperoxy and hydroxy acid products of arachidonate lipoxygenase in rabbit skin.
1. The inflammatory effects of hydroperoxy (HPETE) and hydroxy (HETE) acids, synthesized by arachidonic acid lipoxygenases, have been investigated in rabbit skin. 2. High doses (10-20 micrograms) of 5-, 12- or 15-HPETE or the 5,12-di-hydroxy acid, leukotriene B4 (0.1-1 micrograms), caused small but significant increases in plasma exudation following intra-dermal injection. 3. Leukotriene B4 was equipotent with prostaglandin E2 and prostacyclin in potentiating bradykinin-induced plasma exudation, and was 100 times more active in this property than any other lipoxygenase product tested. 4. Leukotriene B4-induced plasma exudation was enhanced by prostaglandin E2. 5. The mono-HETEs were relatively inactive in causing or enhancing plasma exudation. 6. Leukotriene B4 (0.1 microgram) or prostaglandin E1 (1.0 micrograms) significantly elevated leukocyte accumulation in rabbit skin, whereas PGE2, 5-HPETE, 5-HETE, 12-HPETE or 12-HETE were inactive at doses up to 1 microgram.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D001920:Bradykinin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000494:pharmacology; D005122:Exudates and Transudates / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced*; D007962:Leukocytes / Q000187:drug effects; D007975:Leukotriene B4; D015289:Leukotrienes; D008054:Lipid Peroxides; D011458:Prostaglandins E / Q000494:pharmacology; D011817:Rabbits; D012867:Skin / Q000187:drug effects*
D001095:Arachidonic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; D011458:Prostaglandins E; D007975:Leukotriene B4; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D011464:Epoprostenol; D001920:Bradykinin