Recombination between two IS/s flanking the r-determinant of R100-1: involvement of dor and recA gene functions in Salmonella typhimurium.
Drug resistance genes of the r-determinant component of a composite R plasmid R100-1 were frequently lost in Salmonella typhimurium. Various deletion mutants were analyzed by restriction endonuclease cleavage, Southern blotting, and hybridization techniques. The loss of the r-determinant was found to be the result of a reciprocal recombination between the two IS/s flanking the r-determinant. This recombination depended upon both dor and recA gene functions.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D015246:Deoxyribonuclease EcoRI; D005798:Genes, Bacterial; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011815:R Factors; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI