Conditions for sliding of nucleosomes along DNA: SV 40 minichromosomes.
'Sliding' of nucleosomes along DNA under nearly physiological conditions was studied using treatment of SV 40 minichromosomes with the single-cut restriction endonucleases EcoRI and BamHI. Each enzyme can convert no more than 20-25% of the circular DNA molecules of minichromosomes into the linear form irrespective of the presence of histone H1. This suggests absence of the nucleosomes lateral migration (sliding) along DNa at least in the vicinity of the restriction endonucleases cleavage sites during several hours of incubation. The sites available for EcoRI and BamHI in minichromosomes seem to be located predominantly in the spacer DNA regions of nucleosomes. Introduction of only one double-strand (but not single-strand) break into the DNA of minichromosomes stripped of histone H1 is sufficient to induce redistribution of the nucleosome core particles due to their sliding along DNA. Thus, sliding of the nucleosome core particles can be induced under physiological conditions by rather low energy expenditures.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D007668:Kidney; D008854:Microscopy, Electron; D009707:Nucleosomes / Q000378:metabolism* / Q000648:ultrastructure; D013539:Simian virus 40 / Q000235:genetics*
D004270:DNA, Circular; D004279:DNA, Viral; D009707:Nucleosomes; D004262:DNA Restriction Enzymes