ClaI. a new restriction endonuclease from Caryophanon latum L.
From Caryophanon latum L site specific restriction endonuclease (ClaI) has been purified, which recognises tha DNA hexanucleotide palindrome 5'-A-T-C-G-A-T-3'. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A CLaI map of bacteriophage lambda has been determined, which indicates cleavage inhibition due to adenine methylation at over lapping ClaI-GATC recognition sequences. Plasmid pBR322 is cut only once, in the tetracycline promoter region, and can, therefore, be used as a vector system for cloning fragments derived from ClaI digestions, and in addition for fragments generated by TaqI, HpaII, and several other enzymes.
D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D007777:Lactobacillaceae / Q000201:enzymology*; D010957:Plasmids; D013379:Substrate Specificity
D004262:DNA Restriction Enzymes; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific