A rapid method for the separation of rat pancreatic islets from collagenase-digested pancreas using Percoll.
The isolation of pancreatic islets from collagenase-digested Wistar rat pancreas was shown by the sedimentation method at a unit gravity using Percoll solution with a density of 1.041 g/ml. The density of digested exocrine tissues was in the range of 1.013-1.041 g/ml, while that of purely isolated islets was in the narrow range of 1.066-1.075 g/ml. More than a hundred islets were obtained freely from each rat pancreas without any gross contamination of digested exocrine tissues. A significant increase was observed in glucose-stimulated insulin release from islet isolated with Percoll in the same pattern as that without Percoll. In addition to the well preserved morphology and function of pancreatic islets isolated by Percoll, the simplicity of the technique strongly commends the usefullness of this method.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism*; D011205:Povidone; D051381:Rats; D011919:Rats, Inbred Strains; D012822:Silicon Dioxide
D007328:Insulin; C016039:Percoll; D012822:Silicon Dioxide; D003012:Microbial Collagenase; D011205:Povidone; D005947:Glucose