[Studies on influence of synthetic LH-RH and dbcAMP on normal chorionic villi and BeWo cells (author's transl)].
The influence of synthetic LH-RH and dbcAMP on human chorionic villi and BeWo cells was studied through measurement of cAMP, hCG and estradiol. The results obtained were as follow: 1) There was a rapid increase of cAMP in chorionic villi after the 7th week of gestation, marking a maximum value at the 9th week, and gradually decreasing thereafter. 2) When dbcAMP was added to chorionic villi, there was a significant increase estradiol production in chorionic villi and hCG secretion in media. 3) The stimulation of cAMP in chorionic villi and hCG secretion into medial resulting from the addition of synthetic LH-RH to chorionic villi showed significant increases at 5 minutes and 30 minutes, respectively. 4) When dbcAMP or synthetic LH-RH was added to BeWo cells, increased secretion of hCG and estradiol into media was seen. It therefore is concluded that there is a relationship between the activity of synthetic LH-RH in which cAMP participates and a mechanism involving hCG secretion and estradiol production originating from human chorionic villi and BeWo cells. This is turn suggest the possibility of the existence in chorionic villi of a LH-RH like substance which plays a role in the production or secretion of hormone in the placenta.
D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism* / Q000473:pathology; D006063:Chorionic Gonadotropin / Q000378:metabolism; D002824:Chorionic Villi / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
D006063:Chorionic Gonadotropin; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D003994:Bucladesine; D000242:Cyclic AMP