Murine leukemia virus-mediated transformation of a mouse epithelial cell line MMC-E.
Mouse embryo epithelial cells, MMC-E, were malignantly transformed in culture with MuLV derived from C3H/MCA 5 cells chemically induced by 5-iododeoxyuridine. The frequency of transformation was significantly enhanced if infection was followed by treatment with 100 ng of 12-O-tetradecanoylphorbol-13-acetate per ml. The virus-transformed cells retained their high plating efficiency in soft agar in the absence of 12-O-tetradecanoylphorbol-13-acetate and formed neoplasms in nude mice.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004622:Embryo, Mammalian; D004848:Epithelium; D007065:Idoxuridine / Q000494:pharmacology; D009052:Leukemia Virus, Murine / Q000235:genetics*; D007942:Leukemia, Experimental / Q000382:microbiology; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D012513:Sarcoma, Experimental / Q000382:microbiology
D007065:Idoxuridine