Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus.
A cell line transformed by Abelson murine leukemia virus, called PD, is capable of carrying out kappa-gene rearrangement while growing in culture. Subclones of PD have diverse kappa-gene structures, and some derivatives show evidence of continued joining activity after as many as three subclonings. Analysis of PD sublineages has shown that a rearranged chromosome can undergo secondary kappa-gene rearrangements, producing either a new rearrangement or a deletion of C kappa. Although the PD line actively rearranges its kappa genes, its rearranged heavy-chain genes show little variation, and there is no rearrangement of lambda genes. In PD subclones, DNA fragments representing the reciprocal product of kappa-gene rearrangement are often evident, and they may undergo either further rearrangement or deletion. The implications of multiple rearrangements on a single chromosome and of the maintenance of reciprocal fragments are considered in the context of a model that postulates that the V kappa and J kappa segments are not all organized in the DNA in the same transcriptional direction, leading to inversions rather than deletions during joining.
D000011:Abelson murine leukemia virus / Q000235:genetics*; D000483:Alleles; D000818:Animals; D000916:Antibody Diversity; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D005796:Genes; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains