A zymodeme study of Entamoeba histolytica in a group of South African schoolchildren.
Using a biphasic culture medium, stocks of intestinal amoebae were isolated from a group of children attending school in Durban, South Africa. These were compared with stocks collected in other areas of the world already characterized using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM) L-malate: NADP+ oxido-reductase (oxalacetate-decarboxylating) (ME) and hexokinase (HK). 33% of 94 samples grew Entamoeba histolytica, only one of which gave a pattern indicative of a pathogenic stock. Entamoeba hartmanni, Dientamoeba fragilis and Entamoeba coli were also grown from some samples, increasing the total positive samples for all species isolated to 40%.
D000293:Adolescent; D000562:Amebiasis / Q000469:parasitology*; D002648:Child; D004029:Dientamoeba / Q000201:enzymology; D004747:Entamoeba / Q000201:enzymology; D004748:Entamoeba histolytica / Q000201:enzymology*; D004749:Entamoebiasis / Q000469:parasitology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*
D007527:Isoenzymes