title: In vitro models of viral-induced congenital deafness.
abstract: Cytomegalovirus (CMV) infects 1 to 2 percent of liveborn infants in the United States and causes varying degrees of perceptive hearing loss. There are eight reported pathologic studies of temporal bones in CMV-infected neonates. Viral replication occurs in nonsensory endolabyrinthine epithelium, but viral antigen is also found in the organ of Corti and spiral ganglion neurons, and CMV has been cultured from perilymph. Further clinicopathologic correlation is frustrated, since the inner ear cannot be biopsied during life, and the number of temporal bones available for study is limited, owing to the decrease in the number of autopsies being performed. Inoculation of CMV into newborn mice, and extracorporeal preparations of mouse and guinea pig fetal inner ears, either in organ culture or as grafts on chick chorioallantoic membranes, yields viral perilabyrinthitis. The different ultrastructural appearances of CMV replicating in epithelial and mesenchymal cells show that animal CMV replicates in mesenchymal cells and human CMV replicates in epithelial cells of the inner ear. These different ultrastructural patterns indicate that the chromophobe (transitional) cells of the stria vascularis of the guinea pig are of mesenchymal origin.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001485:Basement Membrane / Q000382:microbiology; D003587:Cytomegalovirus / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000382:microbiology; D003638:Deafness / Q000151:congenital*; D006168:Guinea Pigs; D007759:Labyrinth Diseases / Q000150:complications*; D007762:Labyrinthitis / Q000150:complications* / Q000382:microbiology; D051379:Mice; D008854:Microscopy, Electron; D010498:Perilymph / Q000382:microbiology; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral