Primary culture of normal rat adrenocortical cells. I. Culture conditions for optimal growth and function.
Rat adrenocortical cells retained their differentiated characteristics over 2 wk in culture without a specific requirement for additives other than inorganic salts, amino acids, vitamins, and fetal bovine serum. The cells were maintained free from fibroblast overgrowth by substitution of D-value in place of L-valine in the medium. Corticotropin (ACTH) inhibited the growth of adrenocortical cells in this medium and the effect was reversible. The adrenocortical cells had a limited capacity for growth as reflected by total cell counts and [3H]thymidine uptake with cells from young animals demonstrated a greater potential for DNA synthesis than cells obtained from mature animals. A very sensitive assay for ACTH using a small number of cells in primary culture also is described.
D000302:Adrenal Cortex / Q000166:cytology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D046508:Culture Techniques / Q000379:methods*; D008297:Male; D051381:Rats; D012492:Salts / Q000032:analysis; D014815:Vitamins / Q000032:analysis
D000596:Amino Acids; D003470:Culture Media; D012492:Salts; D014815:Vitamins; D000324:Adrenocorticotropic Hormone