Effects of cyclic nucleotides on ornithine decarboxylase activity in mammary gland explants from mid-pregnant mice.
Dibutyryl cAMP and prolactin stimulated ornithine decarboxylase activity in mouse mammary gland explants which had been preincubated with insulin and cortisol for 1 day; maximally stimulatory concentrations of dibutyryl cAMP and prolactin produced a response which was greater than the sum of the responses of prolactin and dibutyryl cAMP when tested alone. 8-Bromo-cGMP inhibited ornithine decarboxylase activity whereas other derivatives of cyclic nucleotides were without effect. Cortisol concentrations were found to be important for optimizing the dibutyryl cAMP and prolactin responses. Optimal prolactin responses were obtained with cortisol concentrations greater than 10(-7) M, whereas optimal dibutyryl cAMP responses were observed with cortisol concentrations less than 10(-7) M. Despite the differing optimal cortisol concentrations for the prolactin and dibutyryl cAMP responses, it is concluded that prolactin and dibutyryl cAMP probably stimulate ornithine decarboxylase activity in the mammary gland via the same mechanism.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000378:metabolism*; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D009712:Nucleotides, Cyclic / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal; D011388:Prolactin / Q000494:pharmacology
D009712:Nucleotides, Cyclic; D003994:Bucladesine; D011388:Prolactin; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D006854:Hydrocortisone