Immunochemical identification of products of the mitochondrial cytochromoxidase gene expressed in E. coli cells.
Bacterial clones containing hybrid plasmids with mitochondrial DNA insertions were obtained. Among them, a clone synthesizing immunoreactive protein with molecular weight 45,000 was discovered by means of antibodies against mitochondrial cytochromoxidase. The synthesis of the protein depended on orientation of the mitochondrial DNA insertion in the hybrid plasmid. The size of the protein was in excess of the coding capacity of the insertion, which speaks of chimeric nature of the protein. Absorption of the protein by the immunoglobulin fraction containing antibodies against cytochromoxidase resulted in a loss of antibody binding to cytochromoxidase subunit III.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004272:DNA, Mitochondrial / Q000235:genetics*; D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D051381:Rats
D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D003576:Electron Transport Complex IV; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI