title: 23,24,25-Trihydroxyvitamin D3, 24,25,26-trihydroxyvitamin D3, 24-keto-25-hydroxyvitamin D3, and 23-dehydro-25-hydroxyvitamin D3: new in vivo metabolites of vitamin D3.
abstract: Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions, the metabolites were identified as 23,24,25-trihydroxyvitamin D3, 24,25,26-trihydroxyvitamin D3, 24-keto-25-hydroxyvitamin D3 and 23-dehydro-25-hydroxyvitamin D3.
mesh_terms: D000818:Animals; D002112:Calcifediol / Q000031:analogs & derivatives*; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002762:Cholecalciferol / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006887:Hydroxycholecalciferols / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006887:Hydroxycholecalciferols; D002762:Cholecalciferol; C026582:25-hydroxy-24-oxocholecalciferol; C033630:24,25,26-trihydroxyvitamin D3; C033632:23-dehydro-25-hydroxyvitamin D3; C033629:23,24,25-trihydroxyvitamin D3; D002112:Calcifediol