Mutagenesis in Chinese hamster cells by cyclopenta(a)phenanthrenes activated by a human hepatoma cell line.
The cyclopenta(a)phenanthrene, 15,16-dihydro-11-methyl-cyclopenta(a)phenanthren-17-one, had potent mutagenic activity in cell-mediated mutation assays with V79 Chinese hamster cells as targets, and cells of the human hepatoma line HepG2 as mediators of activation. The compound was inactive when low-passage hamster embryo cells were used as activators. When the mutagenic activity of a series of cyclopenta(a)phenanthrenes was compared in mutation assays with HepG2 cells as activators, there was a good correlation between mutagenic activity in this system and carcinogenic activity in mouse skin in vivo. One exception was a noncarcinogenic compound, which is mutagenic in the Ames' test, and was also mutagenic in the mammalian cell assay.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008113:Liver Neoplasms; D008168:Lung / Q000187:drug effects*; D009152:Mutagenicity Tests / Q000379:methods; D010616:Phenanthrenes / Q000494:pharmacology*
D010616:Phenanthrenes