Studies of calcium channels in rat clonal pituitary cells with patch electrode voltage clamp.
1. The properties of the Ca channel in tissue cultured clonal cells (GH(3)) isolated from a rat anterior pituitary tumour were studied with the patch electrode voltage-clamp technique.2. To isolate the current through the Ca channel, the currents through the Na channel, the delayed K channel and the Ca(2+) induced K channel were minimized by replacing the external Na(+) with TEA(+) and adding EGTA to the K-free solution inside the patch electrode.3. The selectivity ratios through the Ca channel with different cations were 2.7 (Ba(2+)):1.6 (Sr(2+)):1.0 (Ca(2+)) and the m(2) form of the activation kinetics and the relationships between the time constant and the membrane potential were common to the three divalent cations.4. The amplitude of the Ba(2+) current increased linearly with [Ba(2+)](o) up to 25 mM and thereafter tended to show saturation.5. The current-voltage relation showed a positive shift along the voltage axis as [Ba(2+)](o) increased, probably due to the screening effect of Ba(2+) on the negative surface charges.6. The time constant of activation as a function of the membrane potential showed a parallel shift as [Ba(2+)](o) was increased, suggesting that the activation kinetics were independent of the permeant ion concentration.7. The time constant of the tail current was consistent with m(2) kinetics for opening and closing of the Ca channel.8. The extrapolated ;instantaneous' tail current rapidly increased as the activating membrane potential became more positive and reached an apparent saturation at membrane potentials substantially more positive than the potential that gave the maximum peak inward current, and suggested that the single channel has a sigmoidal current-voltage relationship.9. The power density spectrum obtained during the steady-state inward Ba(2+) current had a cut-off frequency which was nearly voltage independent; this is expected if the fluctuation of the current originates from m(2) activation kinetics.10. The results of noise analysis suggest that the amplitude of the single Ca channel current was about 0.2 pA at 25 mM-Ba(2+) and 0.7 pA at 100 mM-Ba(2+) for membrane potentials in the vicinity of the maximum inward current.
D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002999:Clone Cells / Q000378:metabolism; D004553:Electric Conductivity; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008564:Membrane Potentials; D008839:Microelectrodes; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism
D007473:Ion Channels; D001464:Barium; D012964:Sodium; D011188:Potassium; D002118:Calcium