Possible role of acetylcholinesterase in regulation of postsynaptic receptor efficacy at a central inhibitory synapse of Aplysia.
Most of the effects of acetylcholinesterase (AChE) on synaptic transmission are considered to be related to its acetylcholine (ACh) hydrolysing properties. This is clearly apparent from changes which occur in the characteristics of the miniature endplate potential and of the endplate potential at neuromuscular junctions when AChE is inhibited1-4 and during the development of enzymatic AChE activity at maturing synapses5. However, we report here that after inhibiting AChE in a cholinergic synapse in Aplysia, we found an increase not only in postsynaptic responses to presynaptic stimulation and to ionophoretic application of ACh on postsynaptic receptors, but also to ionophoretic application of carbachol. This could not be explained by the inhibition of the ACh hydrolysing function of the enzyme, as carbachol is not hydrolysed by AChE. A possible explanation of these observations is that inhibition of the enzyme affects a property of the ACh receptor (AChR) itself.
D000110:Acetylcholinesterase / Q000502:physiology*; D000818:Animals; D001048:Aplysia / Q000502:physiology*; D002712:Chlorides / Q000502:physiology; D002800:Cholinesterase Inhibitors; D005724:Ganglia / Q000502:physiology; D007473:Ion Channels / Q000502:physiology; D009433:Neural Inhibition; D011950:Receptors, Cholinergic / Q000187:drug effects; D017981:Receptors, Neurotransmitter / Q000502:physiology*
D002712:Chlorides; D002800:Cholinesterase Inhibitors; D007473:Ion Channels; D011950:Receptors, Cholinergic; D017981:Receptors, Neurotransmitter; D000110:Acetylcholinesterase