A DNA-nicking activity associated with the nucleocapsid of human cytomegalovirus.
Purified human cytomegalovirus nucleocapsids are able to transform supercoiled plasmid DNA into nicked circular DNA. The activity exhibits a ionic strength optimum of 0.15 M; has a broad pH and temperature dependence and is enhanced after pre-incubation of the nucleocapsids with NP40.
D002213:Capsid / Q000378:metabolism*; D003587:Cytomegalovirus / Q000201:enzymology*; D004270:DNA, Circular / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013696:Temperature; D014764:Viral Proteins / Q000378:metabolism*
D004270:DNA, Circular; D004278:DNA, Superhelical; D014764:Viral Proteins