Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.
A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translated in vivo in Xenopus laevis oocytes and in vitro in reticulocyte and in wheat germ cell-free protein synthesizing systems.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005260:Female; D005796:Genes; D009865:Oocytes / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000138:chemical synthesis*; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic; D014908:Triticum / Q000378:metabolism; D014981:Xenopus
D012315:RNA Caps; D004262:DNA Restriction Enzymes