Effect of the carbon source and cyclic AMP on isocitrate dehydrogenase, succinate dehydrogenase, and malate dehydrogenase in Klebsiella pneumoniae C3.
When strain C3 of Klebsiella pneumoniae is grown on a minimal medium with excess glucose, isocitrate dehydrogenase, malate dehydrogenase, and succinate dehydrogenase specific activities increase in the last period of the exponential growth phase and in the beginning of the stationary phase. Glucose exhaustion does not alter the development of malate dehydrogenase and succinate dehydrogenase, but specific activities are higher than those obtained with excess glucose. In contrast, glucose exhaustion can be correlated with a decrease of isocitrate dehydrogenase specific activity in the stationary phase. Induction of strain C3 isocitrate dehydrogenase by glucose in complex medium and repression by cAMP in mineral medium were observed. Glucose induction and the NADP/NADPH ratio are suggested as regulatory mechanisms controlling isocitrate dehydrogenase synthesis in the Enterobacteriaceae, but the former appears to be restricted to some Klebsiella strains.
D002241:Carbohydrates / Q000494:pharmacology; D002264:Carboxylic Acids / Q000494:pharmacology; D002952:Citric Acid Cycle; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology*; D004794:Enzyme Repression; D005947:Glucose / Q000494:pharmacology; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000254:growth & development; D008291:Malate Dehydrogenase / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D002241:Carbohydrates; D002264:Carboxylic Acids; D003470:Culture Media; D000242:Cyclic AMP; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D005947:Glucose