title: Construction of yeast strains containing genetically marked transposons.
abstract: Haploid yeast cells contain approximately 35 Ty (transposon yeast) elements. To facilitate the study of these elements, we have constructed yeast strains in which one of these transposons carries a genetic marker. The elements that we have modified are Ty912 and Ty917, elements originally detected at the HIS4 locus in spontaneously occurring his4- mutants. The strain constructions took place in three stages: 1) cloning of the mutant HIS4 genes containing the Ty elements; 2) introduction of a HindIII restriction fragment containing the yeast URA3 gene into the cloned transposons; and 3) replacement of the chromosomal HIS4 gene with the modified genes constructed in vitro. These strains will be extremely useful in the study of Ty transposition and other Ty-promoted DNA rearrangements.
mesh_terms: D002875:Chromosomes; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant; D005796:Genes; D010957:Plasmids; D015003:Yeasts / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes