Increases in cyclic AMP potentiate competence formation in BALB/c-3T3 cells.
The ability of platelet-derived growth factor and fibroblast growth factor to stimulate the initiation of DNA synthesis in quiescent BALB/c-3T3 cells was enhanced by cholera toxin. However, the addition of cholera toxin to unsupplemented medium was not mitogenic, nor did cholera toxin increase the mitogenic potential of medium supplemented with platelet-poor plasma. The enhancement of serum-induced DNA synthesis by cholera toxin was due to a specific effect on competence formation and not plasma-controlled progression. Cholera toxin increased the rate of competence formation during a transient exposure of quiescent cells to platelet-derived growth factor; this rate was further increased by the addition of isobutylmethylxanthine, a cyclic nucleotide phosphodiesterase inhibitor. Intracellular cyclic AMP concentrations in quiescent BALB/c-3T3 cells were increased 2- to 3-fold after the addition of cholera toxin., The addition of cholera toxin plus 30 microM isobutylmethylxanthine caused an even greater (7- to 8-fold) increase in the cellular levels of cyclic AMP. That these increases in cyclic AMP concentrations mediated at least part of the increased sensitivity of quiescent cells to competence factors was substantiated by the observation that 0.01 to 1 mM monobutrylcyclic AMP or 8-bromocyclic AMP also caused a concentration-dependent potentiation of competence formation in quiescent cells during a transient exposure to platelet-derived growth factor.
D015124:8-Bromo Cyclic Adenosine Monophosphate; D000818:Animals; D003994:Bucladesine / Q000031:analogs & derivatives / Q000494:pharmacology; D002453:Cell Cycle; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D003260:Contact Inhibition; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000494:pharmacology; D007399:Interphase; D007700:Kinetics; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor
D006133:Growth Substances; D010455:Peptides; D010982:Platelet-Derived Growth Factor; C002373:monobutyryl cyclic AMP; D015124:8-Bromo Cyclic Adenosine Monophosphate; D005346:Fibroblast Growth Factors; D003994:Bucladesine; D004247:DNA; D002772:Cholera Toxin; D000242:Cyclic AMP