Net sodium and glucose transport in the jejunum, ileum and colon of anaesthetized rats in response to intraluminal theophylline and anionic surfactants.
Osmotically balanced solutions of sodium chloride and glucose (5-150 mmol/l) were instilled into rats prepared with two tied intestinal loops (jej-col or il-col). Luminal accumulation or disappearance of Na and glucose after 15 min. was determined, and the parameters of the linear regression lines of net Na flux (y) with initial Na concentration (x) calculated. Control cation and glucose transport were changed by dioctylsulphosuccinate and dodecylsulphate in the way described by Sund & Matheson (1978). Theophylline (10-25 mmol/l) on the other hand did not alter glucose disappearance, and had a distinctly dissimilar effect on net Na transport compared to the surfactants. This could be described as a parallel displacement of the control regression lines to the right, without improvement in correlation. This effect of theophylline was greatest in the ileum, where mean luminal Na concentration corresponding to zero net transport was raised from about 70 mmol/l under control condition to values above 200 mmol/l. The results are consistent with the view that theophylline mainly affects transcellular and the surfactants mainly paracellular sodium transport, and do not support the theory that the effect of dioctylsulphosuccinate on intestinal transport is related to an activation of the mucosal adenylate cyclase/cAMP system, at least not in short term experiments.
D000818:Animals; D000838:Anions; D001692:Biological Transport / Q000187:drug effects; D001834:Body Water / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology*
D000838:Anions; D013501:Surface-Active Agents; D012964:Sodium; D013806:Theophylline; D000242:Cyclic AMP; D005947:Glucose