Immunocytochemical labeling of Anaplasma marginale Theiler in Dermacentor andersoni Stiles with peroxidase- antiperoxidase technique.
Colonies of Anaplasma marginale in midgut epithelial cells of adult ticks that had been infected as nymphs were specifically labeled, using the unlabeled antibody peroxidase-antiperoxidase method of immunocytochemistry. Visual comparison of infected and control tissue sections with the electron microscope demonstrated deposition of ring-like peroxidase-antiperoxidase complexes over organisms within the colonies. The intensity of labeling differed among organisms within a single colony, possibly as a result of varying antigenicity. The labeling observed on organisms in the colonies was similar to that seen on anaplasmal initial bodies in inclusions of infected bovine erythrocytes examined concurrently.
D000709:Anaplasma / Q000302:isolation & purification* / Q000648:ultrastructure; D000818:Animals; D002417:Cattle; D003870:Dermacentor / Q000382:microbiology*; D004064:Digestive System / Q000382:microbiology; D004848:Epithelium / Q000382:microbiology; D004912:Erythrocytes / Q000382:microbiology; D007124:Immunoenzyme Techniques; D002479:Inclusion Bodies / Q000382:microbiology; D013987:Ticks / Q000382:microbiology*