Studies of enteric coronaviruses in a feline cell line.
Development is reported of a feline cell line which can support the growth of coronaviruses from canine (CCV), feline (FIPV) and porcine (TGEV) species. The cell culture has been serially transferred over 100 times and has retained its initial growth requirements, proliferative capacity and morphologic features. Each virus had specific growth characteristics in this cell culture although all produced a similar CPE and plaques under agar. Cross neutralization studies demonstrated a two-way relationship between TGEV and CCV and between TGEV and FIPV, whereas a one-way relationship was demonstrated between CCV and FIPV.
D000818:Animals; D002415:Cats / Q000196:embryology / Q000382:microbiology; D002460:Cell Line; D003332:Coronaviridae / Q000254:growth & development* / Q000276:immunology; D003429:Cross Reactions; D003588:Cytopathogenic Effect, Viral; D004285:Dogs / Q000382:microbiology; D006257:Head / Q000196:embryology; D007422:Intestines / Q000196:embryology; D009500:Neutralization Tests; D005760:Transmissible gastroenteritis virus / Q000254:growth & development / Q000276:immunology; D010948:Viral Plaque Assay; D014776:Virus Cultivation