Maturation of the envelope glycoproteins of Newcastle disease virus on cellular membranes.
Based on subcellular fractionation data, the following maturation pathways were proposed for the Newcastle disease virus glycoproteins. During or shortly after synthesis in rough endoplasmic reticulum, hemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins underwent dolichol pyrophosphate-mediated glycosylation, and HN assumed a partially trypsin-resistant conformation. HN began to associate into disulfide-linked dimers in rough endoplasmic reticulum, and at least one of its oligosaccharide side chains was processed to a complex form en route to the cell surface. During migration in intracellular membranes, F0 was proteolytically cleaved to F1.2. Neither HN nor F1,2 required oligosaccharide side chains for migration to plasma membranes, and cleavage of F0 also occurred without glycosylation. Virion- and plasma membrane-associated HN contained both complex and high-mannose oligosaccharide chains on the same molecule, and F1,2 contained at least high-mannose forms. Several of the properties of HN were notable for a viral glycoprotein. The oligosaccharide side chains of HN were modified very slowly in chick cells, whereas those of the G glycoprotein of vesicular stomatitis virus were rapidly processed to a complex form. Therefore, their different rates of migration and carbohydrate processing were intrinsic properties of these glycoproteins. Consistent with its slow maturation, the HN glycopolypeptide accumulated to high levels in intracellular membranes as well as in plasma membranes. Intracellular HN contained immature oligosaccharide side chains, suggesting that it accumulated in the pre-Golgi/Golgi segment of the maturation pathway. The major site of accumulation of mature HN with neuraminidase activity was the plasma membrane.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D006389:Hemagglutinins, Viral / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism*; D009522:Newcastle disease virus / Q000254:growth & development*; D009844:Oligosaccharides / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000378:metabolism*
D006389:Hemagglutinins, Viral; D009844:Oligosaccharides; D011498:Protein Precursors; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D009439:Neuraminidase