Anchorage-independent colony formation of SV40 transformed BALB/c-3T3 cells in serum-free medium: role of cell- and serum-derived factors.
The colony-forming response of SV40 transformed BALB/c-3T3 cells in agarose suspension culture was studied in a serum-free medium (with insulin, transferrin and serum albumin as the only macromolecular supplements) that was optimized for colony formation of fibronectin-attached monolayer cultures. In this serum-free medium, the SV3T3 cells fail to form colonies in agarose suspension. However, they can be induced to anchorage-independent colony formation by the growth factors that are additionally required by their untransformed counterparts for proliferation in monolayer culture. The SV3T3 cells are also rendered anchorage-independent for colony formation in serum-free medium by conditioned medium from dense monolayer serum-free SV3T3 cultures. These experiments suggest that it is the cell-substrate interaction that is responsible for the growth factor autonomy of fibronectin-attached transformed cells.
D000818:Animals; D001769:Blood; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division; D002472:Cell Transformation, Viral; D003470:Culture Media; D005260:Female; D005353:Fibronectins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013539:Simian virus 40
D003470:Culture Media; D005353:Fibronectins