[Use of isofocusing for isolating and characterizing phosphorus metabolism enzymes].
A modified procedure is described for colorimetric estimation of phosphate in a sample containing ampholines; the procedure enabled to simplify the detection of some enzymes of phosphorus metabolism in the fractions obtained after isoelectric focusing of the initial protein mixture. The analyses did nor require an isotope technique and dialysis and were carried out within a relatively short period.
D000665:Ampholyte Mixtures / Q000494:pharmacology; D000818:Animals; D007525:Isoelectric Focusing / Q000379:methods*; D009206:Myocardium / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000302:isolation & purification*; D010758:Phosphorus / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats
D000665:Ampholyte Mixtures; D010758:Phosphorus; D010749:Phosphoprotein Phosphatases
Ispol'zovanie izofokusirovaniia dlia vydeleniia i kharakteristiki fermentov fosfornogo obmena.