Two Fe2+ atoms are present in activated prolyl 4-hydroxylase.
A linear relationship was found between the activity of prolyl 4-hydroxylase (EC 1.14.11.2) and the amount of Fe2+ bound per mol enzyme. At maximal activity (2.1 mumol X min-1 per mg protein) the enzyme contains 2.1 mol Fe2+ specifically bound per mol enzyme tetramer, indicating two Fe2+-binding sites on prolyl 4-hydroxylase. The half-maximal concentration of added Fe2+ for enzyme activity depends on the nature of sulphydryl compounds present in the reaction medium.
D000818:Animals; D001665:Binding Sites; D002642:Chick Embryo; D007501:Iron / Q000032:analysis*; D007700:Kinetics; D046911:Macromolecular Substances; D010101:Oxygen Consumption; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D011485:Protein Binding
D046911:Macromolecular Substances; D007501:Iron; D011393:Procollagen-Proline Dioxygenase