A "liver" antigen associated with avian erythroblastosis: binding by bentonite and precipitation with sodium dodecyl sulphate.
The properties of a complement fixing antigen, EbAg, extracted from erythroblastosis-affected chicken livers are described. The antigen in extracts freed of structural protein is strongly bound by bentonite, but not by barium sulphate. Strongly alkaline solutions of sodium dodecyl sulphate are required to release the antigen from bentonite. Acidification of the detergent solution precipitates the active solution precipitates the active protein. Extraction of heme from the acidified detergent precipitate by methyl-ethyl ketone further purifies the antigen. This acid detergent treatment eliminates the need to use bentonite as a purification step.
D000327:Adsorption; D001355:Alpharetrovirus / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification* / Q000378:metabolism; D001353:Avian Leukosis / Q000382:microbiology; D001354:Avian Leukosis Virus / Q000276:immunology*; D001466:Barium Sulfate / Q000378:metabolism; D001546:Bentonite / Q000378:metabolism*; D002645:Chickens / Q000382:microbiology; D008099:Liver / Q000276:immunology*; D011201:Poultry Diseases / Q000382:microbiology; D011485:Protein Binding; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism*
D000956:Antigens, Viral; D001546:Bentonite; D001466:Barium Sulfate; D012967:Sodium Dodecyl Sulfate