title: [The comparative mapping of virion and cloned DNA of the hepatitis B virus].
abstract: The entire hepatitis B virus (HBV) genome and its fragments have been cloned into the BamHI site of the plasmid pBR322 vector. The identity of physical maps of cloned and authentic virion DNAs was demonstrated by restriction enzyme analysis. The location of restriction sites is suggestive of a certain similarity between the studied HBV DNA and HBV DNA, subtype ayw (Galibert et al., 1979). From the restriction enzyme analysis of virion DNA repaired and 32P-labeled by the endogenous DNA-polymerase reaction, the new information concerning the location and maximal length (approximately 1500 nucleotides) of the single-stranded region of HBV DNA has been established.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D010957:Plasmids; D014771:Virion / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes
vernacular_title: Sravnitel'noe kartirovanie virionnoĭ i klonirovannoĭ DNK virusa gepatita B.