A new method that investigates superoxide versus respiration in vitro using bioluminescence and Sepharose-bound adenosine derivatives.
In vitro reactions between superoxide and phosphate derivatives of adenosine yielded quantitative amounts of stable ozonide-products. ADP-ozonide was formed in an optimized in vitro synthesis from ADP + O-(2) and affected by inhibitors and uncouplers in a similar manner to in vivo, oxidative-phosphorylation results. ADP-ozonide was further reacted with phosphoric acid to form ATP. Superoxide and ADP-ozonide may be important carrier-intermediates between respiration's electron-transport chain and nodule ATP formation in vivo.
D000241:Adenosine / Q000031:analogs & derivatives*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D008930:Mitochondria, Liver / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010126:Ozone / Q000378:metabolism; D051381:Rats; D012685:Sepharose; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D010126:Ozone; D012685:Sepharose; D000241:Adenosine; D010100:Oxygen