title: Ultrastructural localization of glucose-6-phosphatase and alkaline phosphatase in the vaginal epithelium of rat.
abstract: The effect of ovarian hormones on the activities of glucose-6-phosphatase and alkaline phosphatase in the vaginal epithelium was studied in immature and ovariectomized rats, using ultracytochemical techniques. Comparative studies were done on normal rats at the luteal phase and on day 14 of pregnancy. Various vaginal cells show different degrees of response to progesterone and diethylstilbestrol (DES) with regard to glucose-6-phosphatase activity. Intense glucose-6-phosphatase activity was observed in the cisternae of granular endoplasmic reticulum (rER), Golgi saccules and vesicles, and nuclear envelope of both basal cells and stromal cells of progesterone treated rats, whereas in the basal cells and stromal cells of DES-treated and control animals the enzyme was totally lacking. Detectable glucose-6-phosphatase activity was also observed, however, in the rER cisternae and Golgi complex of keratohyalin-secreting squamous intermediate cells of the vaginal epithelium of DES-treated rats. Alkaline phosphatase was also found on the limiting membranes of secretory granules of mucocytes in animals at the luteal phase and during pregnancy. DES and progesterone in the doses used did not affect alkaline phosphatase activity in the rat vagina. Overall, progesterone enhances glucose-6-phosphatase activity in basal cells of the rat vagina prior to completion of mucification. Alkaline phosphatase was found in all cells involved in mucin secretion.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D004054:Diethylstilbestrol / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006651:Histocytochemistry; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014621:Vagina / Q000187:drug effects / Q000201:enzymology*
chemical_list: D011374:Progesterone; D004054:Diethylstilbestrol; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase