title: A DNA-mediated transformation system for Dictyostelium discoideum.
abstract: We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10(-6) and 10(-4) of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004023:Dictyostelium / Q000235:genetics*; D004351:Drug Resistance; D005822:Genetic Vectors; D009355:Neomycin / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D000617:Aminoglycosides; D004251:DNA Transposable Elements; D004247:DNA; D009355:Neomycin