title: Casein kinase in cytosol of rat and mouse mammary epithelial cells isolated from histone kinase by MgCl2 treatment and influence of pregnancy and lactation on the enzyme activity.
abstract: Rat mammary glands contain cyclic AMP-independent casein kinase and cyclic AMP-dependent histone kinase. The former was easily isolated from cyclic AMP-dependent histone kinase by MgCl2 treatment. Mammary casein kinase was not activated by cyclic nucleotides, and Mg++ and ATP were required for activation. The specific activity of casein kinase in cytosol of rat mammary epithelial cells increased 2 to 3-fold during pregnancy and lactation. Cytosol of mouse mammary epithelial cells also contained cyclic AMP-independent casein kinase, and the activity of this enzyme was about three times that of the Golgi fraction.
mesh_terms: D000818:Animals; D047388:Casein Kinases; D003600:Cytosol / Q000201:enzymology*; D004586:Electrophoresis; D004847:Epithelial Cells; D005260:Female; D066298:In Vitro Techniques; D007774:Lactation; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D010766:Phosphorylation; D011247:Pregnancy; D011270:Pregnancy, Animal; D006656:Protamine Kinase / Q000378:metabolism*; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015636:Magnesium Chloride; D011494:Protein Kinases; D047388:Casein Kinases; D006656:Protamine Kinase; D008274:Magnesium