title: Stimulation by thyrotropin, cholera toxin and dibutyryl cyclic AMP of the multiplication of differentiated thyroid cells in vitro.
abstract: Primary cultures of dog thyroid cells have been established. The cells originated from follicles and displayed differentiation characteristics of such cells: iodide trapping and organification, responsiveness of iodide organification and cyclic AMP accumulation to thyrotropin (TSH), induction of a two-dimensional follicular structure by TSH. TSH also stimulated the multiplication of these cells. The effect of TSH was detected with concentrations as low as 100 muU/ml and was reproduced with purified TSH. It was reproduced by cholera toxin (10 ng/ml) and dibutyryl cyclic AMP (10(-5) M). The data show that TSH, which stimulates the function of thyroid tissue, in vivo and in vitro, activates the multiplication of differentiated dog-thyroid follicular cells in primary culture, which suggests that this trophic effect is, partly at least, mediated by cyclic AMP.
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002449:Cell Aggregation; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology*; D004285:Dogs; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects*; D013972:Thyrotropin / Q000494:pharmacology*
chemical_list: D003994:Bucladesine; D013972:Thyrotropin; D002772:Cholera Toxin