title: Estimation of molecular weights of membrane proteins in the presence of SDS by low-angle laser light scattering combined with high-performance porous silica gel chromatography. Confirmation of the trimer structure of porin of the E. coli outer membrane.
abstract: An assessment study was carried out to evaluate the performance of the low-angle laser light-scattering technique combined with high-performance porous silica gel chromatography in the presence of sodium dodecyl sulfate and precision differential refractometry. It was found that the combined technique is highly promising as a reliable method for determining the molecular weight of a membrane protein solubilized by the surfactant. As a test, molecular weights of porin forming the permeability channel of the outer membrane of E. coli B in an oligomeric form were measured before and after heat treatment, which is known to cause dissociation. The results obtained indicate that the porin oligomer is a trimer with stoichiometric composition.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000032:analysis*; D008027:Light; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D018272:Porins; D012542:Scattering, Radiation; D012967:Sodium Dodecyl Sulfate
chemical_list: D008565:Membrane Proteins; D018272:Porins; D012967:Sodium Dodecyl Sulfate