title: A reproducible method for the optimum infection of human mononuclear cells and lymphoid cell lines by herpes simplex virus type 1.
abstract: Parameters for the infection of human mononuclear cells (MNC) with herpes simplex virus type 1 (HSV-1) were investigated and a procedure was established which resulted in a reproducible optimum number of cells expressing virus-specific cell surface antigens. The number of cells infected was independent of sex of the donor and independent of whether the donor was HSV-seropositive or -seronegative. On the average 18 +/- 6% of HSV-infected MNC from any given donor expressed HSV-specific cell surface antigens. When the standard procedure was applied to a variety of lymphoid cell lines, a high percentage of cells of both B and non-T/non-B lines expressed HSV-specific cell surface antigens, whereas T-cell lines appeared resistant to HSV infection.
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D012737:Sex Factors; D018139:Simplexvirus / Q000276:immunology / Q000502:physiology*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral