Identifying human cells capable of metabolizing various classes of carcinogens.
Human cells that appear capable of metabolizing various classes of carcinogens have been identified using one of two methods: metabolism of tritiated benzo(a)pyrene to aqueous-acetone soluble forms or inhibition of cellular DNA synthesis. Each of the assay systems was optimized and the results on 15 human epithelial cell lines were compared. One or more cell lines were found to activate each of four classes of carcinogens examined: polycyclic hydrocarbons, aromatic amines, heterocyclic hydrocarbons, and nitrosamines. Cells that appeared capable of metabolizing polycyclic hydrocarbons or aromatic amines by these methods were also found to produce metabolites which were cytotoxic to cocultivated human xeroderma pigmentosum fibroblasts after a 48-hr exposure to the carcinogen.
D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004848:Epithelium; D006801:Humans; D006838:Hydrocarbons / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009369:Neoplasms / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D014983:Xeroderma Pigmentosum / Q000378:metabolism
D001580:Benzopyrenes; D002273:Carcinogens; D006838:Hydrocarbons; D001564:Benzo(a)pyrene