Tissue specificity and organisation of CpG methylation in calf satellite DNA I.
Examination of bovine satellite DNA I methylation within CpG dinucleotides has been made by restriction analysis. It is shown that variations in the methylation patterns occur between different tissues (brain, liver, thymus and sperm) . Some of the 8 Hpa II sites present per repeat are clearly undermethylated in sperm as compared to other tissues. Methylation is considered therefore, as a highly specific event. It is also shown that there is a spatial specificity in the methylation pattern of the 3 Hha I sites in all tissues. These results are discussed in the light of methylation and satellite DNA functions.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D003568:Cytidine Monophosphate / Q000031:analogs & derivatives / Q000032:analysis*; D003597:Cytosine Nucleotides / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000302:isolation & purification; D015226:Dinucleoside Phosphates; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D008745:Methylation; D012091:Repetitive Sequences, Nucleic Acid; D013094:Spermatozoa / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D003568:Cytidine Monophosphate; D003597:Cytosine Nucleotides; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite; D015226:Dinucleoside Phosphates; D006151:Guanosine; C015772:CpG dinucleotide