Histocompatibility antigens on astrocytoma cells.
Biopsies tumour cells from astrocytoma-bearing patients were grown in primary culture for 3-5 days. Both low and high grade tumours were represented in the study. The cultured cells could be shown to express the HLA-A and -B antigens using a multispecific allo-antiserum and a rabbit anti-beta-2 microglobulin antibody. The tumour cells were negative for the HLA-DR determinants when tested with either rabbit anti-Ia-like antisera or specific anti-HLA-DR allo-antisera. They also failed to stimulate allogeneic lymphocytes in primary mixed lymphocyte-tumour cell cultures but stimulated lymphocytes primed to tumour cells in vitro. The tumour cells were also capable of stimulating autologous lymphocytes from the tumour-bearing patient in most of the combinations tested.
D001254:Astrocytoma / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005909:Glioblastoma / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II