title: Enzymes related to fructose utilization in Pseudomonas cepacia.
abstract: Growth of Pseudomonas cepacia on fructose, mannitol, or sorbitol depended on formation of an inducible fructokinase (forming fructose-6-phosphate) and the presence of enzymes of the Entner-Doudoroff pathway. Mutants deficient in any of these enzymes failed to utilize the aforementioned carbohydrates. Fructokinase deficiency did not affect growth of the bacteria on glucose. Fructose was accumulated intracellularly by active transport. Mutants blocked in transport of fructose grew normally on mannitol or sorbitol despite their inability to utilize fructose. Growth on either of these hexitols or on galactitol was accompanied by induction of two hexitol dehydrogenases, one active primarily with mannitol and the other active with sorbitol and galactitol. As expected,  a mutant deficient in mannitol dehydrogenase failed to utilize mannitol as a carbon and energy source but grew normally on sorbitol and galactitol. Extracts of bacteria grown on fructose, mannitol, or sorbitol and higher levels of phosphoglucose isomerase than extracts of bacteria grown on alternate carbon sources such as citrate or phthalate. The higher levels were due to appearance of a second phosphoglucose isomerase species not present in cells with the lower activity. The results indicate that the initial steps in fructose utilization by P. cepacia differ from those of most other pseudomonads, which transport fructose by phosphoenolpyruvate-dependent translocation, forming fructose-1-phosphate, and suggest that degradation of fructose, mannitol, and sorbitol occurs primarily via the Entner-Doudoroff pathway.
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D004790:Enzyme Induction; D005631:Fructokinases / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism*; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013012:Sorbitol / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: D005942:Gluconates; D013403:Sugar Phosphates; D005632:Fructose; D008353:Mannitol; D013012:Sorbitol; D008354:Mannitol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase; D010770:Phosphotransferases; D005631:Fructokinases; C021470:2-keto-3-deoxygluconate aldolase; D000446:Aldehyde-Lyases; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase; D005956:Glucose-6-Phosphate Isomerase