title: Human type I procollagen genes are located on different chromosomes.
abstract: A recombinant plasmid containing sequences complementary to human pro-alpha l(I) collagen mRNA was used for the chromosomal assignment of the pro-alpha l(I) collagen gene. Restriction endonuclease analysis of DNA from mouse-human and Chinese hamster-human somatic cell hybrids revealed cosegregation with human chromosome 17. Hybrids containing derivative chromosomes with a t(2;17)(q14;q21) translocation showed cosegregation of the pro-alpha l(I) gene with the segment 17q21 leads to qter. In situ hybridization on human metaphasic chromosomes confirmed this conclusion.
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D002902:Chromosomes, Human, 16-18; D002906:Chromosomes, Human, 6-12 and X; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D011347:Procollagen / Q000235:genetics*
chemical_list: D011347:Procollagen; D004262:DNA Restriction Enzymes