Isolation of zymogen granules from rat pancreas and characterization of their membrane proteins.
A zymogen granule fraction has been isolated from rat pancreas, and its purity has been assessed by biochemical and morphological criteria. Specific activities of two marker enzymes, amylase and chymotrypsin, are increased by 4.6 and 5.4-fold, respectively, as compared to the homogenate. The purified fraction is devoid of detectable RNA, DNA and 5'-nucleotidase, glucose-6-phosphatase, and cytochrome c oxidase activities. Electron micrographs confirm the absence of mitochondria, lysosomes, and rough endoplasmic reticulum fragments. Zymogen granule membranes were isolated from this fraction on a sucrose gradient following lysis in alkaline buffer. Secretory contaminants were efficiently removed from the membranes as indicated by experiments in which labeled secretory proteins were added during the isolation procedure and secondly by measuring residual levels of amylase and chymotrypsin. Three enzyme activities were found in the membranes: thiamine pyrophosphatase, ATP-diphosphohydrolase, and low levels of acid phosphatase. Membrane proteins were solubilized by urea-Triton X-100 and separated in double-dimension (isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Isoelectric point and molecular weight of each protein band were determined.
D000818:Animals; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000032:analysis* / Q000648:ultrastructure; D004792:Enzyme Precursors; D007425:Intracellular Membranes / Q000032:analysis*; D007526:Isoelectric Point; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D010179:Pancreas / Q000648:ultrastructure*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004792:Enzyme Precursors; D008565:Membrane Proteins; D010744:Phosphoric Monoester Hydrolases