Establishment of a human cell line after transformation by a plasmid containing the early region of the SV40 genome.
A human clonal cell line, designated TAH9, has been established from cells of adipose tissue by fusion with E. coli protoplasts containing a recombinant plasmid carrying the early genes of Simian virus 40. The establishment of the cell line is preceeded by a delayed growth crisis. The results indicate that, after cell transformation, another event(s) of low frequency is required for cell immortalization. Some biological properties of TAH9 cells are also described.
D000273:Adipose Tissue / Q000166:cytology; D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D002472:Cell Transformation, Viral; D006801:Humans; D007223:Infant; D008297:Male; D010957:Plasmids; D013539:Simian virus 40