Production of exoenzyme S by clinical isolates of Pseudomonas aeruginosa.
Exoenzyme S differs from toxin A and diphtheria toxin in that it does not adenosine diphosphate (ADP)-ribosylate elongation factor-2, but rather catalyzes the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide to a number of different proteins in extracts of eucaryotic cells. Polyoma-transformed BHK-21 cells were isolated which were resistant to diphtheria toxin and toxin A. Extracts from these cells are ADP-ribosylated by exoenzyme S but not toxin A or diphtheria toxin, providing an assay which distinguishes between S and A activities. A total of 124 clinical isolates of P. aeruginosa were analyzed for production of toxin A and exoenzyme S. Exoenzyme S production was detected in 38% of the strains, whereas 80% of the strains produced toxin A.
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001427:Bacterial Toxins; D001681:Biological Assay; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D005098:Exotoxins / Q000096:biosynthesis; D009713:Nucleotidyltransferases / Q000096:biosynthesis* / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases; D011120:Polyomavirus; D011506:Proteins / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose; D001427:Bacterial Toxins; D005098:Exotoxins; D009713:Nucleotidyltransferases; D011065:Poly(ADP-ribose) Polymerases; D011506:Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C444820:exoenzyme S