Oxidation of tyrosine to dopachrome by peroxidase isolated from murine melanoma.
Peroxidase, isolated from B16 mouse melanoma, converted tyrosine to dopachrome in the presence of either dopa or dihydroxyfumarate co-factor. A suspended homogenate of cloned, cultured B16 mouse melanoma cells also showed peroxidatic conversion of tyrosine to dopachrome in the presence of dihydroxyfumarate co-factor. The findings confirm previous histochemical, autoradiographic-histochemical, and EM-histochemical studies showing that melanoma peroxidase can convert tyrosine to melanin.
D000818:Animals; D002999:Clone Cells / Q000378:metabolism; D066298:In Vitro Techniques; D045563:Indolequinones; D007211:Indoles / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000378:metabolism; D009195:Peroxidase; D010544:Peroxidases / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D045563:Indolequinones; D007211:Indoles; D007527:Isoenzymes; D008543:Melanins; D011809:Quinones; C001123:dopachrome; D014443:Tyrosine; D010544:Peroxidases; D009195:Peroxidase