Incorporation of spin-labeled ganglioside analogues into cell and liposomal membranes.
Ganglioside analogues (gangliosides) with an electron spin resonance label in a long aliphatic hydrocarbon chain were used to investigate the possible insertion of the sialoglycolipid into the plasma membrane of cells. Three types of ESR signals observed in the labeled glycolipids were distinguished. They characteristically indicate an isotropic tumbling motion of spin label in solution, the micellar state of the glycolipid, and an anisotropic motion in a lipid bilayer. Below CMC, gangliosidoide carrying one aliphatic hydrocarbon chain showed an isotropic tumbling motion. After the gangliosidoide had been incubated with liposomes or blood cells, there was an immediate change to an ESR signal showing an anisotropic motion. The signal was typical of the spin-label in liposomes prepared in the presence of spin-labeled sialoglycolipid. It can be concluded that the gangliosidoide was inserted into the lipid phase of liposomal or cellular membranes from the incubation medium. The overall splitting (2A parallel) of 5SL-gangliosidoides in membranes was larger than those of 5SL-galactosylceramide, 5SL-phosphatidylcholine, and 5SL-stearic acid, though the 2A parallel of 12SL-gangliosidoide was almost the same as those of other lipids having a nitroxide group in the 12-position of an acyl chain. This indicates that the head group movement is restricted in gangliosidoide molecules.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002645:Chickens; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D012756:Sheep; D013113:Spin Labels / Q000138:chemical synthesis
D005732:Gangliosides; D008081:Liposomes; D008563:Membrane Lipids; D013113:Spin Labels