title: Rapid miniaturized chromatography for Tc-99m IDA agents: comparison with gel chromatography.
abstract: Miniaturized chromatography systems for determining free pertechnetate and hydrolyzed reduced 99mTc levels in commercial 99mTc-labeled iminodiacetate (IDA) hepatobiliary radiopharmaceuticals were evaluated and the results compared with gel chromatography column scanning (GCS). Commercial IDA agents were evaluated including 2,6-dimethyl IDA, p-isopropyl IDA, p-butyl IDA, and 2,6-diisopropyl IDA. Of all the chromatography systems evaluated, only Gelman ITLC-SA with 20% NaCl and Gelman ITLC-SG with distilled water correlated with GCS in evaluating free pertechnetate and hydrolyzed reduced 99mTc levels for all IDA radiopharmaceuticals. The miniaturized chromatography procedure, as outlined, is rapid, taking less than 4 min, and can easily be incorporated into the daily quality control program in any nuclear medicine facility.
mesh_terms: D002845:Chromatography / Q000379:methods*; D002850:Chromatography, Gel; D007098:Imino Acids / Q000032:analysis*; D015609:Organotechnetium Compounds; D013670:Sodium Pertechnetate Tc 99m; D013667:Technetium / Q000032:analysis*; D019785:Technetium Tc 99m Disofenin; D019780:Technetium Tc 99m Lidofenin
chemical_list: D007098:Imino Acids; D015609:Organotechnetium Compounds; C024340:technetium Tc 99m (para-butyl)iminodiacetic acid; D019780:Technetium Tc 99m Lidofenin; D013667:Technetium; D013670:Sodium Pertechnetate Tc 99m; C003693:technetium Tc 99m PIPIDA; D019785:Technetium Tc 99m Disofenin