Varicella-zoster virus vaccine DNA differs from the parental virus DNA.
The DNAs of a varicella-zoster virus vaccine and its parental virus were compared by CsCl buoyant density centrifugation and restriction enzyme cleavage analysis. The varicella-zoster virus vaccine DNA showed a heterogeneous buoyant profile and altered restriction enzyme cleavage patterns. These changed properties are probably the result of the accumulation of virus containing defective varicella-zoster virus DNA during extensive cell culture passage of the vaccine virus.
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000032:analysis*
D004279:DNA, Viral; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes