[Method of obtaining an erythrocyte diagnosticum for detecting Cl. perfringens type A enterotoxin].
Erythrocytic immunoglobulin diagnostic reagent for detecting Cl. perfringens enterotoxin in the indirect hemagglutination test has been obtained. The diagnostic reagent has been prepared on the basis of antibodies of Cl. perfringens enterotoxin with the use of acroleinized tanned sheep red blood cells. The indirect hemagglutination test with this reagent is characterized by high specificity and sensitivity (the method permits to detect enterotoxin protein at a concentration of up to 0.005 microgram/ml). The preparation thus obtained is stable and ensures highly reproducible results.
D000818:Animals; D003016:Clostridium perfringens; D004768:Enterotoxins / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006386:Hemagglutination Tests / Q000379:methods; D007114:Immunization; D011817:Rabbits
D004768:Enterotoxins
Metod polucheniia éritrotsitarnogo diagnostikuma dlia obnaruzheniia énterotoksina Cl. perfringens tipa A.