In vitro stimulation of rabbit T lymphocytes by cells expressing herpes simplex antigens.
Lymphocyte stimulation responses to herpes antigens were studied using virus-infected X-irradiated cells. Rabbits were immunized with herpes simplex virus type 1 (strain HFEM) grown in RK 13 cells. For in vitro stimulation assay BHK21 cells were X-irradiated (15 000 rad) and infected with a high m.o.i. of a temperature-sensitive (ts) mutant (N102) of HFEM strain at the non-permissive temperature (38.5 degrees C) of virus. Virus antigens were expressed on the infected cells and there was no leakage of infectious virus into the medium at 38.5 degrees C. T lymphocytes from rabbits immunized with herpes simplex virus were specifically activated by herpesvirus-infected X-irradiated cells; lymph node cells from rabbits immunized with RK13 cells and from non-immune rabbits showed no proliferative response.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D008213:Lymphocyte Activation; D009154:Mutation; D011817:Rabbits; D018139:Simplexvirus / Q000254:growth & development / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013696:Temperature
D000956:Antigens, Viral