Introduction of rat growth hormone gene into mouse fibroblasts via a retroviral DNA vector: expression and regulation.
We have introduced the rat growth hormone gene into mouse fibroblasts via a retroviral DNA vector. The ability of the viral DNA to induce foci in the recipient cells was used as a dominant selection marker. Several copies of rat growth hormone DNA were integrated in the mouse cells. The transformed mouse cells expressed rat growth hormone-specific mRNA and secreted mature rat growth hormone. In rat cells, the expression of this gene is regulated by glucocorticoids. We demonstrate that hormone-dependent regulation transfers with the clone and thus appears to be an intrinsic property of the gene or its RNA products.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D005796:Genes; D005809:Genes, Regulator; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D051379:Mice; D010641:Phenotype; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D014170:Transformation, Genetic
D013006:Growth Hormone