Chromosomal mapping of the simian sarcoma virus onc gene analogue in human cells.
The primate cell-derived transforming gene (v-sis) of simian sarcoma virus (SSV) is represented as a single copy marker within cellular DNAs of mammalian species including human. The human analogue of v-sis can be distinguished from its rodent counterparts by Southern blotting analysis of EcoRI-restricted DNAs. By testing for the presence of the human v-sis-related fragment, c-sis (human), in somatic cell hybrids possessing varying numbers of human chromosome, as well as in segregants of such hybrids, it was possible to assign c-sis to human chromosome 22.
D002472:Cell Transformation, Viral; D002874:Chromosome Mapping; D002904:Chromosomes, Human, 21-22 and Y; D005814:Genes, Viral; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D012190:Retroviridae / Q000235:genetics*; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics*