Evidence for production of an inactive collagenase by fibroblasts from phenytoin-enlarged human gingivae.
When measured by radioimmunoassay, fibroblasts derived from the overgrown gingivae of phenytoin-treated epileptic individuals synthesize and release elevated amounts of collagenase in vitro, as compared to similar-appearing fibroblasts from normal, non-phenytoin-treated persons. However, it appears that much of the immunoreactive enzyme is unable to degrade reconstituted collagen in culture. This preliminary finding in 9 different strains of cells indicates that reduced collagenase activity by a subpopulation of cells may contribute to the development of phenytoin-induced gingival overgrowth.
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D005881:Gingiva / Q000166:cytology*; D005885:Gingival Hyperplasia / Q000473:pathology; D005886:Gingival Hypertrophy / Q000139:chemically induced* / Q000473:pathology; D006801:Humans; D008297:Male; D003012:Microbial Collagenase / Q000032:analysis / Q000378:metabolism*; D010672:Phenytoin / Q000494:pharmacology*; D011863:Radioimmunoassay
D010672:Phenytoin; D003012:Microbial Collagenase