Lysosomal enzyme changes in macrophages from mice given myocrisin and infected with avirulent Semliki Forest virus.
Myocrisin given to mice i.p. causes depression of lysosomal enzyme activity (acid phosphatase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase) in peritoneal macrophages. If avirulent Semliki Forest virus (SFV) is given i.p. 3 h after the Myocrisin, further depression of lysosomal enzyme activity occurs, a very high titre of virus is produced in these macrophages and the virus becomes lethal, causing 100% mortality. The possible interrelationships between depressed lysosomal activity, high virus titres and the production of a lethal virus infection are discussed.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002472:Cell Transformation, Viral; D005966:Glucuronidase / Q000378:metabolism; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D012672:Semliki forest virus / Q000187:drug effects / Q000472:pathogenicity; D014036:Togaviridae Infections / Q000201:enzymology*; D014774:Virulence; D014779:Virus Replication / Q000187:drug effects
D006052:Gold Sodium Thiomalate; D000135:Acid Phosphatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase