Isolation and characterization of cDNA clones for human apolipoprotein A-I.
We have isolated cDNA clones encoding human apolipoprotein (apo) A-I. Twenty putative apo A-I cDNA clones were selected by screening 10,000 clones of an adult human liver cDNA library with an oligonucleotide probe. The probe was a mixture of synthetic 14-base-long DNA oligomers constructed to correspond to the codons for apo A-I amino acids 105-109. Four of these clones were examined further and showed 600- to 800-base-pair (bp) inserts. Preliminary restriction mapping and partial DNA sequence analysis indicated that the shorter inserts were a subset of the longer DNA inserts. DNA sequence analysis of the clone with an insert of approximately equal to 600 bp, designated pAI-113, revealed that it contained a DNA sequence corresponding to apo A-I amino acids 94-243. The DNA base sequence of this clone also contained a standard termination codon, polyadenylylation signal, and poly(A) tail. Partial DNA sequence of a second clone that contained an 800-bp insert, designated pAI-107, showed that it corresponded to apo A-I amino acids 18-243 and also included the 3' untranslated region. Isolation of these cDNA clones will facilitate molecular analyses of apolipoproteins in normal and disease states.
D000595:Amino Acid Sequence; D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005796:Genes; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D004251:DNA Transposable Elements; D008075:Lipoproteins, HDL; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes