Construction of recombinant plasmids containing rat thyroglobulin mRNA sequences.
Two plasmids containing rat thyroglobulin cDNA sequences have been constructed and characterized. A plasmid with a 500-bp insert (pRT6) was isolated and identified as thyroglobulin-specific on the basis of the tissue specificity of the inserted sequence and of its ability to retain thyroglobulin mRNA on a nitrocellulose filter. The cDNA insert in pRT6 was subsequently used to screen a rat thyroid cDNA library constructed with large cDNA. A plasmid was found containing a 1700-bp insert. The polarity and the fidelity of the insert is demonstrated by S1 mapping.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D008297:Male; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013954:Thyroglobulin / Q000235:genetics*; D013961:Thyroid Gland / Q000378:metabolism
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D012333:RNA, Messenger; D004247:DNA; D013954:Thyroglobulin; D004262:DNA Restriction Enzymes