Two new restriction endonucleases from Proteus vulgaris.
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D003851:Deoxyribonucleases; D010179:Pancreas / Q000201:enzymology; D011514:Proteus vulgaris / Q000201:enzymology*; D013379:Substrate Specificity
D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes