In vitro effects of parathyroid hormone on kidney cortical slices: cAMP responses and concomitant inhibition of the Na+ gradient-dependent uptake of phosphate by brush border membrane vesicles isolated from the renal slices.
Mouse renal cortical slices were incubated with parathyroid hormone (30 U/ml) for 2 min. Brush border membrane vesicles isolated from the treated slices had a decreased Na+ gradient-dependent uptake of phosphate. Concomitantly, the hormone elicited the activation of adenylate cyclase, the increase in tissue level of cAMP, and the enhancement of cAMP-dependent protein kinase.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D007473:Ion Channels; D010281:Parathyroid Hormone; D010710:Phosphates; D012965:Sodium Chloride; D012964:Sodium; D000242:Cyclic AMP; D011494:Protein Kinases; D000262:Adenylyl Cyclases