[Independence of the insulin effect from the guanosine-3',5'-monophosphate level in muscle tissue].
The role of cyclic GMP in the insulin effect was investigated using isolated frog sartorii. A study was made of the effect of exogenous cyclic GMP, dibutyryl cyclic GMP, 8-bromo-cyclic GMP on xylose transport, glycogen synthesis and muscle respiration. Only dibutyryl cyclic GMP (1.10(-6) - 10(-4) M) alone was observed to have a stimulating effect on glycogen synthesis and respiration. The xylose transport was but slightly accelerated only following a 20 hours incubation of muscles in the cyclic GMP solution. Cyclic GMP was shown to penetrate the muscle fibres. The cyclic GMP content in muscles was equal to 22.7 +/- 2.0 pM per gram of wet weight. Insulin exerted no effect on cyclic GMP concentration in muscles. The data obtained do not allow to conclude that cyclic GMP may serve as a mediator in realization of the insulin effect on membrane and intracellular processes.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D050260:Carbohydrate Metabolism; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D006003:Glycogen / Q000096:biosynthesis; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D011896:Rana temporaria
D007328:Insulin; C016276:8-bromocyclic GMP; D003995:Dibutyryl Cyclic GMP; D006003:Glycogen; D006152:Cyclic GMP
Nezavisimost' éffekta insulina v myshechnoĭ tkani ot urovnia guanozin-3',5'-monofosfata.