Chloride-dependent stimulation of GABA and benzodiazepine receptor binding by pentobarbital.
Pentobarbital reversibly increased Na+-dependent GABA binding to bovine cerebral cortex membranes. This effect was chloride-dependent. Kinetic analysis of [3H]GABA binding revealed an increase of the apparent number of binding sites. Pretreatment of the membranes with Triton X-100 extremely diminished the effect of pentobarbital on both [3H]GABA and [3H]flunitrazepam binding.
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D007700:Kinetics; D010424:Pentobarbital / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002712:Chlorides; D011956:Receptors, Cell Surface; D011955:Receptors, Drug; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam; D010424:Pentobarbital