De novo methylation and expression of retroviral genomes during mouse embryogenesis.
Retrovirus genomes introduced into mouse zygotes by microinjection of cloned DNA, or into morula stage pre-implantation mouse embryos by infection with Moloney murine leukaemia virus (M-MuLV), became de novo methylated and were blocked in expression. No restriction of virus expression and no de novo methylation were observed when post-implantation mouse embryos were infected with virus. Efficient de novo methylation activity may be an important characteristic of gene regulation in early mouse embryos.
D000818:Animals; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics; D047108:Embryonic Development; D005260:Female; D005786:Gene Expression Regulation; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics; D008745:Methylation; D051379:Mice / Q000196:embryology*; D011247:Pregnancy; D012190:Retroviridae / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral