Antiviral activity of antibiotic-producing marine bacteria.
The stability of poliovirus 1 in estuarine water and sediment was examined. The present data indicated that a 2 log reduction in virus titer at 15 degrees c occurred within 6-7 days in water samples taken from estuarine waters on both sides of the Atlantic Ocean. The antiviral effect decreased significantly when the seawater was subjected to autoclaving but not when it was filtered. That the antiviral activity activity of the seawater was related to the growth activities of microorganisms was corroborated by the isolation of antibiotic-producing marine bacteria that had marked activity against poliovirus (net inactivation greater than or equal to 2 logs within 6-8 days). These organisms retained this activity following repeated subcultivation on laboratory media. Since comparable inactivation rates were observed in cell-free filtrates from these marine strains, extracellular products appear to be involved in the virus-inactivation process. Other enteric viruses, Coxsackie B-5 and ECHO-6, were also inactivated by these marine bacteria. The addition of sediment to natural seawater increased the length of poliovirus survival more than three times over that in seawater alone. However, this was not found under sterile conditions, suggesting that the sediment can protect the viruses from inactivation by the marine microflora.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D018407:Bacterial Physiological Phenomena; D004458:Echovirus 6, Human / Q000254:growth & development; D029822:Enterovirus B, Human / Q000254:growth & development; D017955:Poliovirus / Q000254:growth & development*; D011549:Pseudomonas / Q000502:physiology; D012623:Seawater; D012987:Soil; D014733:Vibrio / Q000502:physiology; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D012987:Soil