Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei.
The Lactobacillus casei gene for dihydrofolate reductase has been cloned in Escherichia coli using the multicopy vector pBR322. A restriction map of the cloned DNA has been prepared. The cloned DNA directs the synthesis of L. casei dihydrofolate reductase in E. coli and confers trimethoprim and methotrexate resistance.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D007780:Lacticaseibacillus casei / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D004269:DNA, Bacterial; D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes