title: Alteration of diabetes and interferon induction by the diabetogenic strain of encephalomyocarditis virus through cell passage.
abstract: The diabetogenic strain of encephalomyocarditis virus (D virus) was propagated in several continuous cell lines. Each virus stock was tested for its ability to produce diabetes in mice and induce L-cell interferon (IFN-beta). The effect of insulin on virus replication and IFN-beta induction was also determined. It was found that the severity of the diabetes and the amount of IFN-beta produced was dependent on the cell line used for virus propagation. Virus synthesis was augmented and IFN-beta production was altered in insulin-treated cell cultures. It is concluded that D virus either consists of more than one virus or that its diabetes and IFN-beta inducing characteristics are unstable.
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000382:microbiology*; D004686:Encephalomyocarditis virus / Q000502:physiology*; D004769:Enterovirus Infections / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
chemical_list: D007328:Insulin; D007370:Interferon Type I