Intracellular localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase in a neuronal cell line as examined by immunofluorescence and cell fractionation.
The enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase, EC 3.1.4.37) occurs not only in myelin fractions and glial cells, but can also be shown to be present in a CNS cell line of neuronal origin (B104). Direct immunofluorescence microscopy of B104 cells with fluorescein isothiocyanate-conjugated rabbit anti-CNPase antibodies shows a discrete and specific intracytoplasmic location of CNPase. Fractionation of the cells was performed by differential centrifugation of a cell homogenate and continuous sucrose density-gradient centrifugation. As monitored by marker enzyme activities, CNPase seems to be associated with endoplasmic reticulum membranes.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D001932:Brain Neoplasms; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D009474:Neurons / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D007106:Immune Sera; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases