title: Restriction endonuclease analysis of varicella-zoster vaccine virus and wild-type DNAs.
abstract: The DNA from several clinical isolates of varicella-zoster virus (VZV) were compared with the DNA from the vaccine strain VZV using three restriction endonucleases: BamHI, BgII, and HpaI. When electrophoresed through an agarose gel, the vaccine DNA digestion pattern was significantly different from the digestion patterns of the wild-type DNAs. Variations in the digestion pattern of the separate clinical isolates were also observed.
mesh_terms: D001426:Bacterial Proteins; D002213:Capsid / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004622:Embryo, Mammalian; D005347:Fibroblasts; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000276:immunology; D006801:Humans; D014765:Viral Vaccines / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C024406:endodeoxyribonuclease HpaI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific