[Isolation of a membranous fraction of toad gastric mucosa].
In the present work the isolation and some characteristics of a membrane fraction from toad gastric mucosa are described. By combining differential centrifugation in a discontinuous gradient of sucrose it was possible to obtain a band at the interphase of 22% sucrose 37%, whose enzymatic and electron microscopic analysis indicated to be a fraction enriched of luminal plasma membranes from oxyntic cells, with very poor contamination by other subcellular organelles. This is a suitable membrane preparation that can be used to perform studied on the mechanisms of acid secretion in toads.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002024:Bufo marinus; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D005753:Gastric Mucosa / Q000201:enzymology / Q000648:ultrastructure*; D009708:Nucleotidases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase; D009708:Nucleotidases; D000251:Adenosine Triphosphatases
Aislamiento de una fracción membranosa de mucosa gástrica de sapo.