Enzyme-linked immunoassay in the diagnosis of leptospirosis in domestic animals using peroxidase-conjugated protein-A.
The ELISA test for detection of antibodies to Leptospirosis in domestic animals was performed using Staphylococcal protein-A coupled to peroxidase in place of antisera to IgG. Genus- and type-specific antigens were extracted with SDS technique from four pathogenic serotypes and two non-pathogenic ones, and they were identified with the aid of ELISA using specific rabbit antisera. Micro-agglutination (MA) and ELISA were compared using a total of 48 positive swine sera and a 100% agreement was obtained, since with sera from 16 dogs clinically suspected of Leptospirosis the ELISA resulted highly more sensitive and precocious than MA in detecting specific antibodies.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007527:Isoenzymes; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D009195:Peroxidase; D010544:Peroxidases; D011817:Rabbits; D013205:Staphylococcal Protein A; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D007527:Isoenzymes; D013205:Staphylococcal Protein A; D010544:Peroxidases; D009195:Peroxidase