title: Rearrangement of repeated DNA sequences during development of macronucleus in Tetrahymena thermophila.
abstract: Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive sequences had the same organization in micro- and macronuclear DNAs as revealed by blot hybridization. On the other hand, the remaining three clones with repetitive sequences had apparently different organization in the two nuclear DNAs. All these repetitive sequences showed a smear on the blot in addition to a number of discrete bands when micronuclear DNA was digested with EcoR I. In macronuclear DNAs, these sequences invariably became one or two bands and the smear disappeared. We conclude that, when a macronucleus develops from a micronucleus, the non-repetitive sequences amplify by more than 20 times with relatively few rearrangement, whereas some selected portions of repeated and/or repeat-contiguous sequences are amplified with rather extensive reorganization.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000502:physiology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013768:Tetrahymena / Q000502:physiology*
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes