[Role of cAMP-dependent phosphorylation of rat uterus nuclear proteins in the mechanism of estradiol action].
In experiments on ovariectomized rats cAMP-dependent phosphorylation of the uterine nuclear tissue proteins under the action of estradiol, histamine and cAMP was studied. It was shown, that phosphorylation of the uterine isolated nuclear proteins under the influence of endogenous proteinkinases increases 6 hours after estradiol, histamine and/or cAMP injection to the animals. Proteinkinase activity of the uterine tissue nuclei are higher, when exogenous histone H1 is used as a substrate. The ability of histone H1, isolated from the uterine tissue 6 hours after estradiol, histamine or cAMP injection, to be phosphorylated in vitro with exogenous cAMP-dependent proteinkinase is lowered, that is likely to be resultant of preceding phosphorylation of these proteins in vivo.
D000818:Animals; D002369:Castration; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006632:Histamine / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D006657:Histones; D009698:Nucleoproteins; D004958:Estradiol; D006632:Histamine; D000242:Cyclic AMP; D011494:Protein Kinases
Rol' tsAMF-zavisimogo fosforilirovaniia iadernykh belkov matki krys v mekhanizme deĭstviia éstradiola.