Augmented immunogenicity of Lewis lung carcinoma by infection with herpes simplex virus type 2.
In vitro, LLT cells sustain HSV-2 replication without evidence of lysis. Simultaneously, multiplication of the cells is stimulated. These xenogenized cells were tested for their immunopotentiating capacity: three-step immunization with xenogenized viable cells conferred significantly augmented transplantation resistance to a challenge graft with 4 x 10(4) intact LLT cells. Latency periods preceding tumor formation were increased and 15% of the mice failed to form primary tumors. Metastasis was likewise decreased and 25% of the mice had healthy lungs. Immunopotentiation, however, did not suffice to significantly protect against a challenge with 6 x 10(4) intact cells. The presence of virus-specific neoantigens on HSV-2-infected viable cells was demonstrated by the progressively increasing number of rejections of 4 x 10(4) xenogenized cells during the successive immunization steps. Immunization with non-viable LLT cells did not augment resistance to challenge.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D005260:Female; D007115:Immunization Schedule; D008175:Lung Neoplasms / Q000276:immunology* / Q000556:secondary; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology* / Q000502:physiology; D014181:Transplantation Immunology; D014779:Virus Replication
D000912:Antibodies, Neoplasm