title: A simple technique for preparation of chicken-embryo-skin cell cultures.
abstract: A simple, rapid technique was developed for preparing chicken-embryo-skin cell cultures utilizing trypsinization of the skin of intact 12-day-old chicken embryos. When cell cultures were inoculated with fowl pox virus, those that consisted of at least 80% epithelial cells yielded a higher virus titer than fibroblast cell cultures.
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques / Q000379:methods*; D003588:Cytopathogenic Effect, Viral; D004847:Epithelial Cells; D005587:Fowlpox virus / Q000254:growth & development; D012867:Skin; D014357:Trypsin / Q000378:metabolism; D014776:Virus Cultivation
chemical_list: D014357:Trypsin