Opiate and opioid peptide binding in rat goldfish: further evidence for opiate receptor heterogeneity.
The binding of a series of 3H-labeled mu, kappa, sigma and delta opioid agonists and an antagonist has been examined in rat and goldfish brain membranes. A variety of treatments, including NaCl, N-ethylmaleimide, trypsin and chymotrypsin show dramatic differences both between the two species and between the different 3H-ligands.
D000818:Animals; D001921:Brain / Q000378:metabolism; D003496:Cyclazocine / Q000031:analogs & derivatives / Q000378:metabolism; D004090:Dihydromorphine / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine; D016314:Ethylketocyclazocine; D006054:Goldfish; D008297:Male; D009270:Naloxone / Q000378:metabolism; D009294:Narcotics / Q000378:metabolism*; D010620:Phenazocine / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism*; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D017480:Receptors, sigma
D009294:Narcotics; D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D017480:Receptors, sigma; D009270:Naloxone; D016314:Ethylketocyclazocine; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C012134:SK&F 10047; D004090:Dihydromorphine; D010620:Phenazocine; D003496:Cyclazocine