Assay of adenylate cyclase in rat brain homogenates and lysed turkey erythrocytes: two different methods give different results with brain but not with erythrocytes.
Experiments showed that the cyclic AMP (cAMP) fraction isolated by alumina or Dowex 50/alumina chromatography from brain adenylate cyclase reaction mixtures contained 32P radioactivity 10-12 times in excess of that which could be accounted for by determination of cAMP using binding assays. No such discrepancy was found when lysed turkey erythrocytes were assayed. This indicated that special precautions must be taken for the purification of 32PcAMP from brain adenylate cyclase assays due to the formation of 32P-labelled contaminants.
D000262:Adenylyl Cyclases / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D000242:Cyclic AMP / Q000032:analysis; D004912:Erythrocytes / Q000201:enzymology*; D010761:Phosphorus Radioisotopes; D051381:Rats; D013045:Species Specificity; D014422:Turkeys
D010761:Phosphorus Radioisotopes; D000242:Cyclic AMP; D000262:Adenylyl Cyclases