Effect of leishmanial excreted factor on the activities of adenylate cyclase from hamster liver and Leishmania tropica.
The carbohydrate segment of the excreted factor of culture cells from L. tropica and L. donovani was shown to interact with the adenylate cyclase from hamster liver. At a concentration of 1 mg/ml the excreted factor inhibited the stimulated activity of the mammalian enzyme by about 50%, independent of the activation by glucagon, 5'-guanylylimidodiphosphate and forskolin, respectively. In contrast to the adenylate cyclase system from hamster liver the enzyme from L. tropica-culture cells was not stimulated by addition of glucagon, 5'-guanylylimidodiphosphate and forskolin. In addition, the activity of the adenylate cyclase from L. tropica was not affected by addition of its endogenous excreted factor.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D007891:Leishmania / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology
D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D000242:Cyclic AMP; D000262:Adenylyl Cyclases