Suppression of superprecipitation of contractile proteins from chicken gizzard muscle by preincubation in the presence of adenosine triphosphate without Ca2+.
Superprecipitation of reconstituted actomyosin composed of smooth muscle myosin, skeletal muscle actin and smooth muscle native tropomyosin was studied. When the actomyosin solution was preincubated in the presence of ATP and the absence of Ca2+, or in the relaxed state, superprecipitation was markedly suppressed. The extent of suppression was correlated with the inhibition of the phosphorylation of the 20,000-dalton light chain of smooth muscle myosin. This is consistent with the theory that the interaction of smooth muscle actomyosin is regulated by the phosphorylation of myosin light chain through a system of myosin light chain kinase and phosphatase. However, further studies showed that the myosin light chain kinase and phosphatase system could not explain the present suppression of superprecipitation, even if a cyclic AMP-dependent protein kinase system was also involved. A new regulatory factor should be taken into account in the regulation of smooth muscle actomyosin interaction.
D000199:Actins / Q000378:metabolism*; D000205:Actomyosin / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D011232:Chemical Precipitation; D002645:Chickens; D000242:Cyclic AMP / Q000494:pharmacology; D005895:Gizzard, Avian / Q000032:analysis; D009130:Muscle, Smooth / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits; D014335:Tropomyosin / Q000378:metabolism*
D000199:Actins; D014335:Tropomyosin; D000255:Adenosine Triphosphate; D000205:Actomyosin; D000242:Cyclic AMP; D009218:Myosins; D002122:Calcium Chloride