Tissue-specificity overrides species-specificity in cytoplasmic cytochrome c oxidase polypeptides.
With a high-resolving dodecyl sulfate electrophoretic system rat liver cytochrome c oxidase was separated into 13 different polypeptides. An antiserum against rat liver holocytochrome c oxidase immunoreacted with all 13 polypeptides, as demonstrated by immunofluorescence after transfer of the separated Coomassie blue-stained bands on nitrocellulose and coupling with FITC-protein A ("western blot"). Polypeptide-specific antisera reacted only with their corresponding polypeptides indicating that the various protein bands are represented by individual polypeptides. From total proteins of rat liver, kidney, heart, spleen and skeletal muscle mitochondria, only the cytochrome c oxidase polypeptides showed immunofluorescence with an antiserum against the rat liver holoenzyme. In contrast to the polypeptide from liver, polypeptide VIa from heart and skeletal muscle showed little or no reactivity, indicating a tissue-specificity of this polypeptide. Mitochondrial proteins from pig, bovine and blackbird heart were incubated with an antiserum against the rat liver holoenzyme. Immunoreaction was found with most cytochrome c oxidase polypeptides but not with polypeptide VIa. This result demonstrates less immunological relationship between tissue-specific polypeptides (VIa, VIIa and VIII) of the same species than between tissue-unspecific polypeptides of different species.
D000818:Animals; D001717:Birds; D002417:Cattle; D003576:Electron Transport Complex IV / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000201:enzymology*; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D008929:Mitochondria, Heart / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009928:Organ Specificity; D010455:Peptides / Q000032:analysis; D051381:Rats; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology*; D013552:Swine
D046911:Macromolecular Substances; D010455:Peptides; D003576:Electron Transport Complex IV