Adenosine 3' :5' monophosphate receptor sites in Xenopus laevis oocytes.
Adenosine 3':5' cyclic monophosphate (cyclic AMP) binds to proteins present in the 100,000 g supernatant fractions of full-grown oocytes of Xenopus laevis. Optimal pH for the binding is 4.0. Two receptor binding sites have been characterized by density gradient centrifugation as peaks with sedimentation constants of 4.6 S and 5.9 S. The apparent dissociation constants for the two cyclic AMP binding sites are 7 nM and 40 nM. The total cyclic AMP binding capacity of oocyte cytosol is 15.8 +/- 2.2 fmoles/oocyte and remains constant during meiotic maturation induced by progesterone.
D000818:Animals; D002499:Centrifugation, Density Gradient; D000242:Cyclic AMP / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008540:Meiosis; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011953:Receptors, Cyclic AMP / Q000302:isolation & purification / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism
D011953:Receptors, Cyclic AMP; D011374:Progesterone; D000242:Cyclic AMP