Lipoprotein apolipoprotein synthesis by human hepatoma cells in culture.
Lipoprotein synthesis was demonstrated by double diffusion with low density lipoprotein antibody, and by 3H-labeled amino acid incorporation into proteins of the d less than 1.063 g/ml centrifugally isolated lipoprotein fraction. Radioactive label was incorporated predominantly into apolipoprotein B (60%), apolipoprotein A-I (20%) and apolipoprotein C (12%), as determined by Sepharose column chromatography and polyacrylamide gel electrophoresis. Incorporation of radioactive label into apolipoprotein B was inhibited by the presence of albumin in the medium, and was restored to control levels with the addition of 1 mM oleic acid, indicating that cell synthesis of apolipoproteins could be modified by culture conditions. The human hepatoma cell line, Hep G2, provides a potential in vitro model for the study of regulation of human hepatic lipoprotein and apolipoprotein synthesis.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001053:Apolipoproteins / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008113:Liver Neoplasms; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000596:Amino Acids; D001053:Apolipoproteins; D009829:Oleic Acids; D012710:Serum Albumin, Bovine; D019301:Oleic Acid