[Kinetic characteristics of microsomal NAD-glycohydrolase natural and solubilized with a non-ionic surface-active substance].
Microsomal rat spleen NAD-glycohydrolase was solubilized by Nonidet P40. The solubilized enzyme shows Nicotinamide inhibition and pH dependence at the same extent as unsolubilized microsomal one. It differs from the latter in having a higher affinity for NAD and NADP, and in showing two peaks, instead of one, on electrofocusing: the former with a pH 5 pI without any activity, the latter with a pH 4, 1 pI with a high NAD-ase activity.
D000818:Animals; D007525:Isoelectric Focusing; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D009536:Niacinamide / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D013154:Spleen / Q000201:enzymology*
D009243:NAD; D009536:Niacinamide; D011092:Polyethylene Glycols; D009249:NADP; D017830:Octoxynol; C010615:Nonidet P-40; D009244:NAD+ Nucleosidase
Caratteristiche cinetiche della NAD-glicoidrolasi microsomiale nativa e solubilizzata con un tensioattivo non ionico.