Presence of double stranded regions of viral RNA in infected cells.
Ribonuclease treatment of rhinovirus-infected human embryo lung cells after cell disruption reveals that double stranded RNA is present in the preparation before nucleic acids are extracted with phenol. This shows that the hydrogen bonding between complementary molecules of viral RNA which occurs in infected cells is not a result of the extraction of RNA with phenol.
D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006860:Hydrogen Bonding; D008168:Lung / Q000196:embryology; D010636:Phenols; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012229:Rhinovirus / Q000502:physiology*; D012260:Ribonucleases
D010636:Phenols; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012260:Ribonucleases