Casein kinase in cytosol of rat and mouse mammary epithelial cells isolated from histone kinase by MgCl2 treatment and influence of pregnancy and lactation on the enzyme activity.
Rat mammary glands contain cyclic AMP-independent casein kinase and cyclic AMP-dependent histone kinase. The former was easily isolated from cyclic AMP-dependent histone kinase by MgCl2 treatment. Mammary casein kinase was not activated by cyclic nucleotides, and Mg++ and ATP were required for activation. The specific activity of casein kinase in cytosol of rat mammary epithelial cells increased 2 to 3-fold during pregnancy and lactation. Cytosol of mouse mammary epithelial cells also contained cyclic AMP-independent casein kinase, and the activity of this enzyme was about three times that of the Golgi fraction.
D000818:Animals; D047388:Casein Kinases; D003600:Cytosol / Q000201:enzymology*; D004586:Electrophoresis; D004847:Epithelial Cells; D005260:Female; D066298:In Vitro Techniques; D007774:Lactation; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D010766:Phosphorylation; D011247:Pregnancy; D011270:Pregnancy, Animal; D006656:Protamine Kinase / Q000378:metabolism*; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D015636:Magnesium Chloride; D011494:Protein Kinases; D047388:Casein Kinases; D006656:Protamine Kinase; D008274:Magnesium