[Oncogen localization in the DNA composition of simian adenoviruses. II. The identification in SV20 virus DNA of a specific fragment possessing transforming activity].
Simian adenovirus 20 DNA was specifically cleavered by restriction endonucleases EcoRI, BamHI, XbaI and HindIII. The transformation activity of the DNA digest was investigated. BamHI, XbaI, and HindII DNA digests were able to transform the primary rat kidney cell culture (Wistar) as well as the native SV20 DNA. The transforming activity was revealed in a specific fragment of the viral DNA, obtained after the treatment of the DNA with BamHI (fragment B), with molecular weight 5.4 x 10(6) dalton. This fragment is located in the left end of the viral genome. The lack of cell transformation by the EcoRI-hydrolysate of viral DNA may serve a proof of the extremely left position of the oncogene in the viral genome, since of EcoRI-fragment chips off a fragment with molecular weight 3 x 10(5) dalton fr om the left side of DNA molecule.
D000256:Adenoviridae / Q000032:analysis*; D000261:Adenoviruses, Simian / Q000032:analysis* / Q000235:genetics; D000818:Animals; D002273:Carcinogens; D002472:Cell Transformation, Viral / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014776:Virus Cultivation
D002273:Carcinogens; D004279:DNA, Viral; D004262:DNA Restriction Enzymes
K voprosu o lokalizatsii onkogena v sostave DNK adnovirusov obez'ian. II. Identifikatsiia v DNK virusa SV20 spetsificheskogo fragmenta, obladaiushchego transformiruiushcheĭ aktivnost'iu.