Effect of tunicamycin on receptors for tumor promoters.
A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.
D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002273:Carcinogens / Q000378:metabolism*; D002352:Carrier Proteins; D004815:Epidermal Growth Factor / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D008358:Mannose / Q000378:metabolism; D051379:Mice; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism*; D010704:Phorbols / Q000378:metabolism*; D011493:Protein Kinase C; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011955:Receptors, Drug; D012867:Skin / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology*
D029742:Caenorhabditis elegans Proteins; D002273:Carcinogens; D002352:Carrier Proteins; D006023:Glycoproteins; D010703:Phorbol Esters; D010704:Phorbols; D011956:Receptors, Cell Surface; D011955:Receptors, Drug; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D014415:Tunicamycin; D015240:Phorbol 12,13-Dibutyrate; D004815:Epidermal Growth Factor; D011493:Protein Kinase C; D005944:Glucosamine; D008358:Mannose