Specific adsorbents for affinity chromatography of benzodiazepine binding proteins.
The use of affinity chromatography as means of isolating/purifying proteins which have an affinity for benzodiazepine is described. Three such drugs are employed: chlorazepate, clonazepam and delorazepam. The results presented in this paper indicate that the proposed technique only works for chlorazepate and delorazepam. In fact these benzodiazepine-Sepharose derivatives are able to retain specifically proteins from human serum and rat kidney, lung, skeletal muscle and brain.
D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D001923:Brain Chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000032:analysis; D008168:Lung / Q000032:analysis; D008565:Membrane Proteins / Q000302:isolation & purification; D009124:Muscle Proteins / Q000302:isolation & purification; D011485:Protein Binding; D051381:Rats; D011955:Receptors, Drug / Q000302:isolation & purification*; D011963:Receptors, GABA-A
D001798:Blood Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D011955:Receptors, Drug; D011963:Receptors, GABA-A; D001569:Benzodiazepines