title: Anomalous reduction of cytochrome b in highly purified complex III from baker's yeast.
abstract: In highly purified bc1-complex from baker's yeast, the reduction of cyt c1 and partial reduction of cyt b is obtained by catalytic amount of succinate dehydrogenase and succinate in the presence of 7 microM antimycin. After the addition of ferricyanide the c1 is re-oxidized and a increase in the reduction of b is observed. Using stopped-flow we established that the oxidation of c1 by ferricyanide proceeds as a pseudo-first order reaction and the reduction of b is faster and with two phases. Our observation suggests that these two processes are not directly interconnected and that other component than c1 must be the "control factor" in the anomalous reduction of cyt b. This component must be, by exclusion, the iron-sulfur protein.
mesh_terms: D003573:Cytochrome b Group; D003580:Cytochromes / Q000302:isolation & purification / Q000378:metabolism*; D003575:Cytochromes c1 / Q000378:metabolism; D014450:Electron Transport Complex III; D007700:Kinetics; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D003573:Cytochrome b Group; D003580:Cytochromes; D009097:Multienzyme Complexes; D003575:Cytochromes c1; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; D014450:Electron Transport Complex III