title: Comparison of the nucleotide sequence of cloned human and guinea-pig pre-alpha-lactalbumin cDNA with that of chick pre-lysozyme cDNA suggests evolution from a common ancestral gene.
abstract: Nucleotide sequence analyses of essentially full-length copies of human and guinea-pig pre-alpha-lactalbumin cDNAs contained within recombinant plasmids, (i) confirm the presence of 19 amino acid hydrophobic amino terminal peptide extensions encoded within each mRNA; and (ii) provides evidence for the existence of a minor variant of guinea-pig alpha-lactalbumin mRNA encoding a protein with a 36 residue carboxyl-terminal extension. Comparison of the nucleotide sequence within the coding region of the human, and the predominant guinea-pig pre-alpha-lactalbumin mRNAs, with the analogous region of hen pre-lysozyme mRNA provides compelling evidence that all have evolved from a common ancestral gene.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis; D004792:Enzyme Precursors / Q000235:genetics*; D005796:Genes; D006168:Guinea Pigs; D006801:Humans; D007768:Lactalbumin / Q000235:genetics*; D009113:Muramidase / Q000235:genetics*; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D013045:Species Specificity
chemical_list: D004274:DNA, Recombinant; D004792:Enzyme Precursors; D011498:Protein Precursors; D012333:RNA, Messenger; D012335:RNA, Ribosomal; C034435:pre-alpha-lactalbumin; D004247:DNA; D007768:Lactalbumin; D004262:DNA Restriction Enzymes; D009113:Muramidase