Interaction between iodothyronines and thyrotropin receptor in human cultured thyroid cells.
In order to verify the existence of a "short-loop" negative feedback between iodothyronines and adenylate cyclase system of human thyroid, we have studied the effect of preincubation with iodothyronines, iodotyrosines, iodothyronine analogues and iodide on TSH-induced cAMP cellular accumulation in normal human thyroid cells in primary culture. Iodide did not produce an inhibitory effect on TSH-dependent adenylate cyclase system both in normal human thyroid plasma membranes and cultured cells. Iodothyronines at a 30-40 microM concentration did not inhibit the TSH-dependent adenylate cyclase activity of human thyroid plasma membranes; however at a 1 microM concentration they were able to inhibit the TSH-dependent cAMP accumulation by cultured cells. Preincubation with iodotyrosines and iodothyronine analogues failed to inhibit the TSH-responsive cAMP accumulation in human thyroid cultured cells.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000302:Adrenal Cortex / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D002478:Cells, Cultured; D015232:Dinoprostone; D006801:Humans; D007454:Iodides / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011989:Receptors, Thyrotropin; D013961:Thyroid Gland / Q000378:metabolism*; D013963:Thyroid Hormones / Q000494:pharmacology*; D013972:Thyrotropin / Q000494:pharmacology
D007454:Iodides; D011458:Prostaglandins E; D011956:Receptors, Cell Surface; D011989:Receptors, Thyrotropin; D013963:Thyroid Hormones; D000324:Adrenocorticotropic Hormone; D013972:Thyrotropin; D000262:Adenylyl Cyclases; D015232:Dinoprostone