Radioiodination of p-hydroxyphenylisopropyladenosine: development of a new ligand for adenosine receptors.
dl-p-Hydroxyphenylisopropyladenosine has been iodinated with 125I and tested as a ligand of adenosine receptors in membranes from rat brain using a filtration assay. Binding studies using l-[3H]phenylisopropyladenosine as a ligand were carried out in parallel, and the binding of both ligands could be displaced by dl-p-hydroxyphenylisopropyladenosine and l-phenylisopropyladenosine in a similar fashion. These data establish the feasibility of using radioiodinated derivatives of adenosine as ligands of suitable types of adenosine receptors.
D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008024:Ligands; D008566:Membranes / Q000378:metabolism; D010660:Phenylisopropyladenosine / Q000031:analogs & derivatives; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011983:Receptors, Purinergic
D007457:Iodine Radioisotopes; D008024:Ligands; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D010660:Phenylisopropyladenosine; C036265:N-(4-hydroxyphenyl)-1-isopropyladenosine; D000243:Adenosine Deaminase