The use of highly concentrated purified (by a large scale method) and long term liquid nitrogen stored foot-and-mouth disease viruses for the preparation of vaccines: physico-chemical quality controls and potency tests after storage.
In 1974 a new industrial technique for concentration and purification of FMD virus was presented at the OIE Conference. The bulk inactivated virus from this technique was stored in liquid nitrogen vapour until required for vaccine formulation. In 1981, having applied this technique regularly for seven years, we now describe the results obtained and the advantages gained in the field of trivalent O, A, C bovine vaccine production. Vaccines prepared from such bulk virus stocks after several years storage give good protection against virulent virus challenge.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D005537:Aphthovirus / Q000302:isolation & purification / Q000502:physiology*; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D004355:Drug Stability; D005615:Freezing; D007668:Kidney; D009584:Nitrogen; D011786:Quality Control; D014612:Vaccines / Q000592:standards*
D000956:Antigens, Viral; D014612:Vaccines; D009584:Nitrogen