T-lymphocyte and B-lymphocyte subpopulations infiltrating human mammary carcinomas.
Both T- and B-lymphocytes were found in human primary mammary carcinomas and were distributed in widely varying amounts, but in most tumors, T-lymphocytes predominated. A small percentage of the T-lymphocytes expressed receptors for the Fc portion of IgG (Fc gamma R), but very few had receptors for C3 (C3+) (comparable to the findings in blood). A prominent subset of lymphocytes had Fc gamma R and were C3+, and most of these were surface immunoglobulin (slg)-bearing cells. The majority of lymphocytes from this subset were Fc+ C3+, and only a small percentage were Fc+ C3- (in contrast to the findings in blood). IgD and IgM were the predominant classes of findings in blood). IgD and IgM were the predominant classes of immunoglobulin found on the B-lymphocytes. The different preparative techniques did not result in a selective loss of lymphocyte subsets, but collagenase digestion did lead to a loss of expression of the C3 receptor on the lymphocyte surface, which was recoverable when lymphocytes were reincubated at 37 degrees C. No evidence was found for blocking of the C3 receptor by immune complexes with activated complement.
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000032:analysis / Q000166:cytology*; D001943:Breast Neoplasms / Q000276:immunology*; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D016177:Macrophage-1 Antigen; D003012:Microbial Collagenase / Q000378:metabolism; D008875:Middle Aged; D011951:Receptors, Complement / Q000032:analysis; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000032:analysis / Q000166:cytology*
D003176:Complement C3; D003181:Complement C4; D007136:Immunoglobulins; D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D003012:Microbial Collagenase