title: Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus.
abstract: The transforming protein of Kirsten murine sarcoma virus (Ki-MuSV) is a virally encoded 21-kilodalton protein called p21 kis. The sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned Ki-MuSV DNA to induce focal transformation of mouse cells. Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D007708:Kirsten murine sarcoma virus / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D015689:Oncogene Protein p21(ras); D012367:RNA, Viral / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D015689:Oncogene Protein p21(ras)