title: Chain assembly intermediate in the biosynthesis of type III procollagen in chick embryo blood vessels.
abstract: A general mechanism for the assembly of procollagens is proposed from a biosynthetic study of procollagen III. This was shown to proceed by a stepwise process punctuated by disulfide bond formation and an assembly intermediate was recovered. The biosynthesis of type III procollagen in excised chick embryo blood vessels was studied by radioactive labeling for 30 min. Velocity sedimentation under denaturing conditions and purified antibodies specific against bovine amino propeptide III were used to identify and characterize monomeric pro alpha 1 III chains and a type III procollagen intermediate which is interchain disulfide-linked only at the carboxyl end but not at the amino end. The monomeric chains presumably have intrachain disulfide bonds within the propeptides. The monomeric pro alpha 1 III chains were also found when alpha, alpha'-dipyridyl was present during incubation. Pulse-chase experiments show that the monomeric chains and the intermediate are biosynthetic precursors of type III procollagen. Furthermore, it is shown that monomeric pro alpha 1 chains are not triple helical when extracted under nondenaturing conditions. The results indicate that the assembly of pro alpha 1 III chains into type III procollagen starts with the association of the folded carboxyl propeptides and is followed by formation of disulfide bonds between carboxyl propeptides, folding of the triple helix, and formation of disulfide bonds between amino propeptides. All procollagens may follow a similar assembly sequence.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D046911:Macromolecular Substances; D003012:Microbial Collagenase; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010434:Pepsin A; D011347:Procollagen / Q000096:biosynthesis*
chemical_list: D046911:Macromolecular Substances; D011347:Procollagen; D010434:Pepsin A; D003012:Microbial Collagenase