Cytochemical localization of some hydrolases in the pollen and pollen tubes of Amaryllis vittata Ait.
Some hydrolases are localized cytochemically in the pollen and pollen tubes of Amaryllis vittata Ait. The function of different enzymes is discussed in relation to pollen tubes morphogenesis. Activity of most of the enzymes was confined to colpus region, pollen wall and general cytoplasm of pollen and pollen tube. The activity of hydrolytic enzymes like acid monophosphoesterase and lipase and was nil in the exine of both germinated and ungerminated pollen, whereas intense reaction for esterase was observed in exine. Enzyme activity increased after germination which suggest the hydrolysis of stored metabolites and synthesis of proteins and other metabolites for the active growth of pollen tube. Intense reaction for enzymes like alkaline phosphomonoesterase, ATP-ase, 5-nucleotidase etc. at the tip region of pollen tube suggest their role in physiological processes associated with exchange of materials through intercellular transport during tube wall polysaccharide biogenesis.
D015720:5'-Nucleotidase; D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D011058:Pollen / Q000201:enzymology*
D006867:Hydrolases; D008049:Lipase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases