Application of chemiluminescent probes in investigating lysosomal sensitivity to superoxide versus suspected radical scavengers.
The role of the superoxide anion radical (O2(-).) relative to catalytic/inhibitory substances in lysosomes is poorly understood. Cultured glial cells sequestered endocytotically two probes that were site-specific to the lysosome vacuome and sensitive to radical activities. The Sepharose-4B-isoluminol probe emitted chemiluminescent light in proportion to externally injected O2(-). and was inhibited or catalysed by various radical scavengers, transition metals and other substances which may affect lysosomal metabolism and lipofuscin formation. We conclude that lysosomal radical activities may be inhibited by butylated hydroxytoluene, hydrocortisone, ACF, RNA, alpha-tocopherol, and, in special circumstances, with fully metabolized iron. Choline and oxidized copper and iron cations in overload concentrations in incubated freshly in lysosomes may catalyse radical activities, and they may be important factors in lipofuscin formation and its role in aging.
D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D002794:Choline / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004705:Endocytosis; D005609:Free Radicals; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007501:Iron / Q000494:pharmacology; D008062:Lipofuscin / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives; D008247:Lysosomes / Q000187:drug effects* / Q000502:physiology; D009457:Neuroglia / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology*; D011134:Polysaccharides; D011724:Pyridazines; D012685:Sepharose / Q000031:analogs & derivatives; D013481:Superoxides / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D007202:Indicators and Reagents; D008062:Lipofuscin; D011134:Polysaccharides; D011724:Pyridazines; D013481:Superoxides; D008165:Luminol; D003300:Copper; C027239:sepharose-4B-polyisoluminol; D012685:Sepharose; D007501:Iron; D005966:Glucuronidase; D002794:Choline; D010100:Oxygen