Two-dimensional NMR spectroscopy of siomycin A. Proton--carbon-13 chemical shift correlation.
A trial application of a recent two-dimensional nuclear magnetic resonance experiment to the polypeptide antibiotic siomycin A is described. Proton--carbon-13 chemical shift correlation measures the proton and carbon-13 chemical shift for each directly bonded CH group in a molecule, in a single experiment. The resultant map of correlated chemical shifts enables the carbon-13 spectrum to be assigned directly from the known proton shifts, and allows individual proton signals to be identified without problems of overlap. The signal-to-noise ratio available from such techniques should enable their application to aqueous protein solutions using currently available high-field spectrometers.
D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000032:analysis; D011522:Protons
D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D010455:Peptides; D011522:Protons; C024711:siomycin A