Poly(adenosine diphosphate ribose) polymerase activity in neuronal and glial nuclei from bovine cerebrum.
Two different preparations isolated from beef cerebrum have been used to compare the polyadenosine diphosphate ribose (polyADPR) polymerase activities in neuronal and glial nuclei: (1) nuclear suspensions (with or without DNase I treatment), and (2) 1 M NaCl nuclear extracts (soluble enzyme). The DNAse I treatment of nuclei and the solubilization of polyADPR polymerase by 1 M NaCl enhances the polyADPR polymerase activity. The polyADPR polymerase activity is similar in neuronal and glial nuclear suspensions, while the neuronal soluble enzyme activity is significantly higher than that of the glial soluble enzyme. Evidence is presented that the difference in soluble enzyme activities is not due to the effects of DNA or degrading enzymes. Some activating factor(s) seem to be present in neuronal soluble extracts, while both inhibiting and activating factor(s) seem to be present in glial soluble extracts.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009457:Neuroglia / Q000187:drug effects / Q000201:enzymology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D012965:Sodium Chloride; D011065:Poly(ADP-ribose) Polymerases; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D009244:NAD+ Nucleosidase