A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli.
A new plasmid cloning vehicle (pDM2) was used to introduce a library of Haemophilus influenzae chromosomal fragments into H. influenzae. Transformants of the highly recombination-defective rec-1 mutant were more likely to contain exclusively recombinant plasmids after exposure to ligated DNA mixtures than was the wild type. pDM2 could replicate in Escherichia coli K-12.
D000667:Ampicillin / Q000494:pharmacology; D003001:Cloning, Molecular; D004261:DNA Replication; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006193:Haemophilus influenzae / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial
D004274:DNA, Recombinant; D000667:Ampicillin