Ionic strength and pH effects in the kinetically controlled synthesis of benzylpenicillin by nucleophilic deacylation of free and immobilized phenyl-acetyl-penicillin amidase with 6-aminopenicillanic acid.
D000581:Amidohydrolases; D004800:Enzymes, Immobilized; D005998:Glycine / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009994:Osmolar Concentration; D010397:Penicillanic Acid / Q000031:analogs & derivatives; D010399:Penicillin Amidase; D010400:Penicillin G / Q000138:chemical synthesis*; D010648:Phenylacetates
D004800:Enzymes, Immobilized; D010648:Phenylacetates; C022050:phenylacetylglycine; D010397:Penicillanic Acid; D000581:Amidohydrolases; D010399:Penicillin Amidase; D010400:Penicillin G; C004408:aminopenicillanic acid; D005998:Glycine