Adenovirus late sequences linked to herpes simplex virus thymidine kinase may be introduced into eukaryotic cells and transcribed.
LTK-cells have been transformed to the TK+ phenotype by treatment with size-defined concatamers of HSV-1 TK DNA and Ad2 Bam H1 C fragment (42.0 - 59.5 map units). All TK+ transformants contained Ad2 DNA as well as HSV-1 TK sequences. In most cases several inserts of virus DNA were present, many in high copy numbers. Although no Ad2 transcription promoter was present in the transforming DNA, Ad2-specific sequences were detected in polyadenylated cytoplasmic RNA species from several cell lines.
D000260:Adenoviruses, Human / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics*; D007739:L Cells / Q000201:enzymology; D051379:Mice; D009693:Nucleic Acid Hybridization; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D013937:Thymidine Kinase