Specific binding of 1,25-dihydroxycholecalciferol in human medullary thyroid carcinoma.
A specific 1,25-dihydroxycholecalciferol-binding protein has been detected in high-salt cytosols prepared from human medullary thyroid carcinomas. The binding protein had the same equilibrium dissociation constant (Kd = 0.17 +/- 0.05 nM; n = 4) and sedimentation coefficient on sucrose gradients (3.7S) as than seen in established vitamin D target tissues. This protein was not detected in normal thyroid cytosols, which may reflect the low proportion of C-cells within the gland.
D002117:Calcitriol / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism*
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol