Increased content of Type V Collagen in desmoplasia of human breast carcinoma.
Type V (AB) collagen is present in increased amounts in desmoplasia of human breast carcinoma. Type V collagen occurs as linear deposits in the interstitium (desmoplastic stroma surrounding the tumor), as demonstrated by immunofluorescence and immunoperoxidase techniques utilizing antibodies to Type V collagen. In contrast, no demonstratable Type V occurs in the interstitium of fibroadenoma, fibrocystic disease, or normal breast tissue. As identified and quantitated by pepsin extraction, salt fractionation, and polyacrylamide gel electrophoresis, Type V constitutes 10% +/- 5% of all interstitial collagens (Types I, III, and V) in desmoplasia, compared with 1.5% +/- 0.5% in fibroadenoma and less than 0.1% in all types of fibrocystic disease, including sclerosing adenosis, and less than 0.1% in normal breast tissue. For infiltrating ductal carcinoma, Type V collagen is not secreted by the invasive carcinoma cells, nor is it present in an extracellular basement membrane location. By immunohistologic methods, Type V collagen can be seen exclusively in the desmoplastic stroma. The authors propose that desmoplasia of human breast carcinoma manifests a characteristic collagen profile and that the increased Type V may be produced by specialized cells such as myofibroblasts in the interstitium, which are recruited in response to invasive carcinoma.
D002293:Adenocarcinoma, Scirrhous / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D000232:Adenofibroma / Q000473:pathology*; D000818:Animals; D001365:Axilla; D001941:Breast Diseases / Q000473:pathology*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis* / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005348:Fibrocystic Breast Disease / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D011817:Rabbits
D003094:Collagen