Iron-sulphur clusters in fumarate reductase from Vibrio succinogenes.
(1) The fumarate reductase complex from Vibrio succinogenes contains one FAD molecule, one [4Fe-4S]3+(3+,2+) and one [2Fe-2S]2+(2+,1+) cluster per enzyme molecule. Both clusters can be partly reduced by succinate. In the presence of excess Na2S2O4 and fumarate, the [2Fe-2S] cluster is completely oxidized, whereas the other cluster is largely reduced. (2) The [2Fe-2S] cluster is localized in the Mr, 31,000 subunit. The EPR spectrum of the reduced cluster in the isolated subunit differs slightly in line width, but not in g-value, from the spectrum of reduced, intact enzyme complex. The demonstrates that the immediate environment of th cluster is little perturbed by dissociating this subunit from the FAD-containing Mr 79,000 subunit. The temperature dependence of the power-saturation behaviour has, however, greatly decreased in the isolated subunit, the saturation at 11 K of the paramagnetic cluster being much less than in the enzyme complex. Moreover, the temperature dependence of th power-saturation behaviour of this cluster in the enzyme is greater with succinate as reducing agent, than with dithionite. (3) The [4Fe-4S] cluster is located on the Mr 79,000 subunit. This cluster is unstable in air when the subunit has been dissociated from the enzyme complex.
D055598:Chemical Phenomena; D002621:Chemistry; D004227:Dithionite; D004578:Electron Spin Resonance Spectroscopy; D005182:Flavin-Adenine Dinucleotide; D005650:Fumarates / Q000494:pharmacology; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D010084:Oxidation-Reduction; D012964:Sodium / Q000494:pharmacology; D013385:Succinate Dehydrogenase; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D013431:Sulfates / Q000494:pharmacology; D013696:Temperature; D014733:Vibrio / Q000201:enzymology*
D005650:Fumarates; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D013386:Succinates; D013431:Sulfates; C012036:sodium sulfate; D005182:Flavin-Adenine Dinucleotide; D004227:Dithionite; D012964:Sodium; D019802:Succinic Acid; D013385:Succinate Dehydrogenase