Inhibition by alpha-amanitin of development of tetrodotoxin-sensitive spike induced by brain extract in cultured chick skeletal muscle cells.
The enhancing effect of brain extract on the development of tetrodotoxin-sensitive sodium channels in cultured chick skeletel muscle cells is blocked by treatment of culture with alpha-amanitin, and RNA polymerase inhibitor. The result is interpreted as indicating that the trophic substance in the brain extract exerts its effect on the development of sodium channels through regulation of gene transcription.
D000200:Action Potentials / Q000187:drug effects; D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D012964:Sodium / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology*; D014020:Tissue Extracts / Q000494:pharmacology*
D000546:Amanitins; D007473:Ion Channels; D014020:Tissue Extracts; D013779:Tetrodotoxin; D012964:Sodium