Separation of viral DNA molecules in horizontal agarose slab gels using a new type of wick.
A new type of rayon fiber wick was used to establish uniform electrical paths between agarose slab gels and the electrodes of a horizontal electrophoresis apparatus. Separation of viral DNA molecules, either digested with restriction enzymes or relaxed with eukaryotic topoisomerases, was achieved easily at both low and relatively high voltage.
D010582:Bacteriophage lambda / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004264:DNA Topoisomerases, Type I / Q000494:pharmacology; D004279:DNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D004264:DNA Topoisomerases, Type I