Fluorescence histochemical methods for the study of peptide hormone-producing cells.
Fluorescence histochemical methods for the demonstration of specific residues in peptides and proteins are reviewed: Formaldehyde-ozone for NH2-terminal tryptophan, formaldehyde-HCl for tryptophan regardless of position in the peptide, OPT for NH2-terminal histidine, formaldehyde-fluorescamine for "protected" amino groups, nitroso-naphthol for tyrosine, and phenanthrenequinone for arginine residues. The methods are potent in demonstrating granule-stored material in peptide hormone-producing cells. Also quinacrine, the fluorescent anti-malaria agent, binds to granular components, as yet unidentified, in several endocrine cell types. In many cases the fluorescence histochemical methods seem to demonstrate peptides and proteins distinct from the known hormones.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000378:metabolism; D005557:Formaldehyde; D005755:Gastrins / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006728:Hormones / Q000378:metabolism*; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D010179:Pancreas / Q000033:anatomy & histology; D010455:Peptides / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000033:anatomy & histology; D051381:Rats; D014364:Tryptophan / Q000378:metabolism
D005755:Gastrins; D006728:Hormones; D010455:Peptides; D005557:Formaldehyde; D006639:Histidine; D014364:Tryptophan; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D005934:Glucagon; D001120:Arginine