Presence of corticotropin releasing factor-like immunoreactivity in hypophysial portal blood.
Hypophysial protal blood was collected from pentobarbital anesthetized male rats, and the content of corticotropin releasing factor-like immunoreactivity (CRF-LI) was measured using an RIA developed for synthetic ovine CRF. The concentration of CRF-LI in portal plasma was 104.9 +/- 9.7 pM. This concentration is in the range which has been shown to stimulate ACTH and beta-endorphin secretion in vitro. These data support the hypothesis that a molecule with the same or similar structure as synthetic ovine CRF is a physiologically significant hypothalamic releasing factor.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000097:blood* / Q000494:pharmacology; D004723:Endorphins / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism; D011168:Portal System; D011863:Radioimmunoassay; D051381:Rats; D001615:beta-Endorphin
D004723:Endorphins; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone