title: [3H]Diazepam displacing activity in human cerebrospinal fluid.
abstract: Pooled human cerebrospinal fluid was separated by Sephadex G-50 chromatography. The presence of three peaks, A, B and C, was demonstrated by monitoring absorbance at 254 and 280 nm. All peaks showed [3H]diazepam displacing activity in the membrane receptor test. Peak B was further separated on Bio-Gel P-4. At least two major fractions free of salt and GABA in the molecular weight range of approximately 700--3600 were shown to displace [3H]diazepam in the receptor test. This activity was enhanced by a factor of 3 in the presence of 10 microM-GABA.
mesh_terms: D000818:Animals; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002555:Cerebrospinal Fluid / Q000502:physiology*; D003975:Diazepam / Q000378:metabolism*; D006801:Humans; D008297:Male; D051381:Rats; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism; D011963:Receptors, GABA-A; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D001688:Biological Products; D011955:Receptors, Drug; D011963:Receptors, GABA-A; D014020:Tissue Extracts; D003975:Diazepam