Human monoclonal autoimmune antibody produced in vitro: rheumatoid factor generated by Epstein-Barr virus-transformed cell line.
The infection of selected lymphocytes from a rheumatoid arthritis patient with Epstein-Barr virus resulted in an immortalized cell line that secretes a monoclonal rheumatoid factor (RF). The cloned line has been growing for more than 24 months, and constantly produces a monoclonal IgM, lambda, 19S, RF (1-2 micrograms/ml/106 cells). The RF agglutinates human and rabbit IgG (but not IgM) and also protein A-coated erythrocytes, but fails to do so to mouse, goat and swine IgG-coated erythrocytes. When bound to immune complexes, this monoclonal RF dose not bind complement. In the cell supernatant RF is the only immunoglobulin and it comprises approximately 5% of the total proteins. The affinity of RF to aggregated human IgG, as detected in inhibition experiments, is higher than that of Fc receptors found on human non-T lymphocytes, K562 and Daudi cell lines.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001172:Arthritis, Rheumatoid; D002460:Cell Line; D002472:Cell Transformation, Viral; D002499:Centrifugation, Density Gradient; D004854:Herpesvirus 4, Human; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008214:Lymphocytes; D012217:Rheumatoid Factor / Q000096:biosynthesis*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D012217:Rheumatoid Factor