title: Plasmid DNA in the erythromycin producing microorganism, Streptomyces erythreus NRRL 2338.
abstract: Streptomyces erythreus NRRL 2338, the erythromycin producing microorganism, contains extrachromosomal (plasmid) DNA. Four different plasmids, pSE1, pSE2, pSE4 and pSE6 present in the wild-type strain have characteristic mobilities on agarose gel electrophoresis, molecular weight and restriction endonuclease digestion patterns. Treatment of the wild-type strain with ethidium bromide or acridine orange gave two variants, one that could not convert erythronolide B to 3 (alpha)-mycarosylerythronolide B and another than produced 2 approximately 3 times more erythromycin A than the parental strain. Although the plasmid DNA profile of these two variants is different from the wild-type strain, it is not possible to conclude that any of the structural genes for erythromycin biosynthesis are located on the plasmids of S. erythreus NRRL 2338.
mesh_terms: D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004917:Erythromycin / Q000096:biosynthesis*; D006868:Hydrolysis; D008970:Molecular Weight; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D004269:DNA, Bacterial; D004917:Erythromycin; D004262:DNA Restriction Enzymes