Insertion of nonhomologous DNA into the yeast genome mediated by homologous recombination with a cotransforming plasmid.
Bacterial plasmids containing no detectable homology with yeast DNA sequences were inserted into the yeast genome by cotransforming with a plasmid containing a yeast gene. Analysis of the yeast transformants confirmed that recombination events occurred between the prokaryotic sequences shared by the two plasmids and between the yeast sequences common to the cotransforming plasmid and to the genome. Multiple copies of the two plasmids, in both tandem and interspersed arrays, are inserted by this method. Populations of cells grown from individual transformants are heterogeneous for the number of integrated sequences. The number of integrated bacterial sequences is greatly reduced after 100 generations of growth in the populations that initially contained large numbers of sequences, while it is stable in those populations that initially contained either a single or a small number of copies.
D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004271:DNA, Fungal; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes