Border disease: tissue culture studies of the virus in sheep.
A California strain of border disease (BD) virus was isolated from the CNS and spleen of an infected lamb and was propagated without cytopathic effect on 2 tissue culture systems. Viral antigen was detected in the cytoplasm of infected cells by immunofluorescence. The growth curve of this BD virus in primary sheep choroid plexus cells revealed a latent period of 12 hours and peak viral production by 36 hours after inoculation. The growth curve in porcine kidney cells (PK-15) demonstrated a latent period of 8 hours and peak viral production by 54 hours after inoculation. The primary sheep choroid plexus cell tissue culture isolate of BD virus was then used to develop hyperimmune sera in 2 adult sheep. Neutralizing antibody was not detected until 43 days after primary inoculation when a slow antibody increase occurred in both sheep.
D000818:Animals; D002831:Choroid Plexus; D007668:Kidney; D010576:Pestivirus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D013552:Swine; D014036:Togaviridae Infections / Q000382:microbiology / Q000662:veterinary*; D014776:Virus Cultivation; D014779:Virus Replication