Crystallization patterns of membrane-bound (Na+ +K+)-ATPase.
Extensive formation of two-dimensional crystals of the proteins of the pure membrane-bound (Na+ +K+)-ATPase is induced during prolonged incubation with vanadate and magnesium. Some membrane crystals are formed in medium containing magnesium and phosphate. Computer-averaged images of the two-dimensional crystals show that the unit cell in vanadate-induced crystals contains a protomeric alpha beta-unit of the enzyme protein. In phosphate-induced crystals an (alpha beta) 2-unit occupies one unit cell suggesting the interactions between alpha beta-units can be of importance in the function of the Na+, K+ pump.
D000818:Animals; D003460:Crystallization; D007668:Kidney / Q000201:enzymology; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D046911:Macromolecular Substances; D008565:Membrane Proteins; D010710:Phosphates; D014639:Vanadium; D014638:Vanadates; D000254:Sodium-Potassium-Exchanging ATPase