Microinjection of nucleic acids into cultured mammalian cells by electrophoresis.
Simian virus 40 (SV40) DNA was microinjected into cultured mammalian cells by means of electrophoresis (iontophoresis). Successful transfer of DNA into cells was confirmed by detecting SV40 T antigen using the indirect immunofluorescent technique.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics*; D004586:Electrophoresis; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology / Q000276:immunology; D008845:Microinjections / Q000379:methods; D013539:Simian virus 40 / Q000235:genetics*
D000956:Antigens, Viral; D004279:DNA, Viral