title: [Isolation and purification of restriction endonuclease BpeI from Bordetella pertussis].
abstract: New restriction endonuclease has been isolated from Bordetella pertussis vaccine strain 305 and purified in 1 stage on Sepharose covalently bound with blue dextran. The isolated restrictase has been found capable of breaking down lambda-phag DNA into 7 fragments. According to its specificity, Bpe I is the isoschizomer of Hind III obtained from Haemophilus influenzae strain Rd.
mesh_terms: D001886:Bordetella pertussis / Q000201:enzymology* / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000302:isolation & purification*; D015247:Deoxyribonuclease HindIII; D005818:Genetic Engineering; D007536:Isomerism
chemical_list: D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII
vernacular_title: Vydelenie i ochistka restriktsionnoĭ ĕndonukleazy Bpel iz Bordetella pertussis.