[Cloning of the promoter of the RNA polymerase operon located before the structural gene of the beta-subunit].
An EcoRI-fragment of the rpoBC operon carrying the end of the rplL gene, the intercistron region and the beginning of the rpoB gene cloned on the pBRH4 plasmid makes it tetracyclin-resistant, i. e. possesses the properties of a promoter. There is no appreciable difference in the degree of antibiotic resistance of recombinant plasmids carrying the main PJ promoter and the additional promoter.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D046911:Macromolecular Substances; D009876:Operon; D010957:Plasmids
D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI
Klonirovanie promotora RNK-polimeraznogo operona, raspolozhennogo pered strukturnym genom beta-sub"edinitsy.