title: Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver.
abstract: (14)CO(2) production by perfused rat livers was simulated by infusing NaH(14)CO(3) into the perfusate. Recovery of label as (14)CO(2) gas + perfusate bicarbonate was 45-85%. Rates of (14)CO(2) exchange in the liver are 3-70 times greater than net rates of CO(2) production. Therefore (14)CO(2) reincorporation can lead to significant underestimations of rates of oxidation of (14)C-labelled substrates in liver.
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002250:Carbon Radioisotopes; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008722:Methods; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D017693:Sodium Bicarbonate
chemical_list: D001639:Bicarbonates; D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D017693:Sodium Bicarbonate