Tricyclic antidepressant desipramine induces stereospecific opiate binding and lipid modifications in rat glioma C6 cells.
Incubation for 48 hours of C6 glioma cell cultures with 10(-4)M tricyclic antidepressant desipramine gave rise to a quantitative increase of total lipids and to qualitative modifications of glycosphinegolipids involving detection by thin-layer chromatography of spots migrating according to cerebroside and sulfatide and presence of an abnormal ganglioside pattern. These lipid modifications were associated with the appearance of stereospecific binding of opiates (dihydromorphine) with a dissociation constant of 30-60 nM. These results favor an important role of lipids in opioid receptor function.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism; D003891:Desipramine / Q000494:pharmacology*; D004090:Dihydromorphine / Q000378:metabolism; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism; D050356:Lipid Metabolism; D009447:Neuroblastoma / Q000188:drug therapy; D051381:Rats; D011957:Receptors, Opioid; D013237:Stereoisomerism
D011957:Receptors, Opioid; D004090:Dihydromorphine; D003891:Desipramine