Generation of BALB-MuSV and Ha-MuSC by type C virus transduction of homologous transforming genes from different species.
The nature of the cell-derived (bas) sequences of BALB-MuSV, a spontaneous mouse sarcoma virus isolate, was determined. Molecularly cloned bas sequences demonstrated no detectable homology with the onc genes of other mouse transforming viruses, but exhibited a high degree of sequence homology with the ras gene of the rat-derived Harvey murine sarcoma virus (Ha-MuSV) genome. The Ha-MuSV cell-derived sequence (ras) shared a colinear 750 bp region of homology with bas. Moreover, BALB-MuSV transformation was associated with the expression of high levels of a 21,000 dalton protein, immunologically related to the ras gene products, p21. Thus bas and ras represent retroviral transforming gene homologs that were independently transduced by mouse type C viruses from the genomes of different species.
D000011:Abelson murine leukemia virus / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D051379:Mice; D008807:Mice, Inbred BALB C / Q000382:microbiology; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012190:Retroviridae / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins