Equine infectious anaemia: detection of antibodies using an immunofluorescence test.
An indirect immunofluorescence test for detecting antibodies to equine infectious anaemia virus is presented. Using monolayers of equine dermal cells within a defined period after infection, discrete fluorescent spots were observed in the cytoplasm of as many as 95 per cent of the cells. These inclusions appeared as ring-like structures when high titred sera were employed but became spots when the sera were diluted. Cells showing optimal antigen fluorescence were used immediately or after storage at -70 degrees C. The fluorescence test detected lower levels of antibody than the immunodiffusion test, and results were available in less than two hours. It should be useful as a confirmatory assay with the immunodiffusion test.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004859:Equine Infectious Anemia / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique; D006736:Horses; D005779:Immunodiffusion / Q000662:veterinary; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*
D000914:Antibodies, Viral