The localization of converting enzyme in kidney vessels of the rat.
An antibody against pure rabbit lung converting enzyme (CE) showing cross-reaction with CE from other species was used for immunocytochemical studies in the kidney of rats. Using the indirect labelling PAP-technique, specific immunostaining was found in the endothelial layer of all arteries and arterioles of kidney cortex and in some descending vasa recta. CE-positive reactions were also seen in most glomeruli, the reaction product being confined to only a few capillary loops in connection with the glomerular stalk. A few immunostained capillaries in the cortical labyrinth were suspected to belong to the first ramifications of the efferent arteriole. The bulk of all other of the glomerular and peritubula capillaries as well as all veins of the kidney showed no obvious immunostaining. The functional significance of this specific localization pattern of CE in the endothelium of kidney vessels is discussed with respect to the actions of the systemic and the local, intrarenal renin-angiotensin-system on kidney functions.
D000818:Animals; D006651:Histocytochemistry / Q000379:methods; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000098:blood supply / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D007703:Peptidyl-Dipeptidase A