Physical mapping of adenovirus type 2 temperature-sensitive mutations by restriction endonuclease analysis of interserotypic recombinants.
The genome structures of about 100 interserotypic ts recombinants produced in crosses between human adenovirus type 2 (H2) and 5 (H5) temperature-sensitive mutants were analyzed by cleavage with restriction endonucleases to determine the map coordinates of the following temperature-sensitive mutants: penton base plus fiber-defective H2 ts103, -104, and -136, assembly-defective H2 ts112, fiber-defective H2 ts125, hexon-defective H2 ts118 and -121, and DNA-negative H2 ts111. H5 ts1 (100 K defective), H5 ts36 (DNA negative), H5 ts125 (mutated in the early 72,000-dalton protein), H5 ts22 (fiber defective), H5 ts58 (IIIa defective), and H5 ts18 and -19 were used as one of the parents. The physical locations of the H2 temperature-sensitive mutations thus defined are discussed in relation to the genetic map, the biological function altered, and the positions of the structural genes on the genome.
D000260:Adenoviruses, Human / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D013696:Temperature
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D004262:DNA Restriction Enzymes