Cryopreservation of platelets isolated with the IBM 2997 blood cell separator: a rapid and simplified approach.
The equivalent of 5-7 units of platelets, isolated from a single donor with the IBM Blood Cell Processor 2997 using a dual stage separation chamber, was frozen with the cryoprotectant dimethylsulfoxide (DMSO). The DMSO-saline solution was added directly to the platelets, and the platelets were frozen in a polyvinyl chloride plastic bag by storage in a -80 degrees C mechanical freezer. Washing the thawed platelets with a phosphate-buffered sodium chloride-dextrose solution, pH of 5.0, removed about 95% of the DMSO. In vitro freeze-thaw-wash recovery was 80%, and in vivo 51Cr platelet recovery was 31%. Platelet dense body granules were well maintained after freezing, thawing, and washing. This is a safe and effective method of platelet cryopreservation which can be performed in less time than other currently used methods.
D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004051:Diethylhexyl Phthalate / Q000097:blood; D004121:Dimethyl Sulfoxide / Q000032:analysis / Q000494:pharmacology; D005615:Freezing; D006801:Humans; D002479:Inclusion Bodies / Q000502:physiology / Q000648:ultrastructure; D008875:Middle Aged; D010976:Platelet Count
D003451:Cryoprotective Agents; D004051:Diethylhexyl Phthalate; D004121:Dimethyl Sulfoxide