title: Immunogenicity of guinea pig cells transformed in culture by chemical carcinogens.
abstract: The immunogenicity of inbred strain 2/N guinea pig fibroblasts transformed to the malignant state in vitro by chemical carcinogens was evaluated with the use of a variety of in vivo and in vitro methods including delayed-type hypersensitivity skin and tumor transplantation tests and analysis of antibody production by immunofluorescence, complement fixation, and staphylococcal protein A binding tests. Neoplastic transformation was induced by direct treatment of cells in culture with benzo[a]pyrene, 3-methylcholanthrene, or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or by the host-mediated method by which fetuses were exposed to diethylnitrosamine or MNNG in vivo prior to cell culture. Rabbits and syngeneic guinea pigs were inoculated with unirradiated and X-irradiated clonally derived cells. Delayed hypersensitivity skin reactions to immunizing or other cells were equivalent in immunized or control guinea pigs, and no protection to tumor outgrowth from a challenge inoculum of immunizing cells was observed. Antibody activity induced in the sera of immunized guinea pigs was cross-reactive and removed by absorption with nontumorigenic cells. Rabbit antisera after absorption with fetal guinea pig cells were nonreactive with the specific immunizing or other culture cells. Chemical carcinogen-induced neoplastic transformation of guinea pig cells can, therefore, occur without formation of detectable, individually distinct cell surface tumor-specific neoantigens.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000276:immunology*; D001564:Benzo(a)pyrene; D001580:Benzopyrenes; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002999:Clone Cells / Q000276:immunology; D003168:Complement Fixation Tests; D005347:Fibroblasts / Q000276:immunology* / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007111:Immunity, Cellular / Q000528:radiation effects; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008748:Methylcholanthrene; D008769:Methylnitronitrosoguanidine; D009368:Neoplasm Transplantation; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D001580:Benzopyrenes; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D008769:Methylnitronitrosoguanidine; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene