Receptor remodeling and regulation in the action of epidermal growth factor.
Epidermal growth factor (EGF) initiates a wide variety of events when added to responsive cultured cells. These range from early events requiring only brief exposure to EGF, e.g., stimulation of transport of amino acids or ions, to later events such as commitment of cells to a round of DNA synthesis, a process requiring 6 h or more of continuous exposure to hormone. EGF binding is followed first by phosphorylation of EGF receptors, which can be detected in purified membranes and permeabilized cells, and then by internalization and proteolytic processing of receptors in lysosomes. Native 160,000-dalton EGF receptors contain a site that is not exposed on the cell surface and is highly sensitive to cleavage by an endogenous protease, which yields a 145,000-dalton receptor fragment that retains phosphate acceptor activity. Cleavage of receptor at a trypsin-sensitive site, also not exposed to the cell surface, yields a 115,000-dalton fragment that binds EGF, but contains no phosphorylated species. The data indicate that the phosphate acceptor sites on EGF receptors are localized on a 45,000-dalton cytosolic region.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004261:DNA Replication; D004815:Epidermal Growth Factor / Q000502:physiology*; D066246:ErbB Receptors; D046911:Macromolecular Substances; D051379:Mice; D008954:Models, Biological; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D011956:Receptors, Cell Surface / Q000502:physiology*; D013997:Time Factors
D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D010447:Peptide Hydrolases