The effect of GTP on benzodiazepine receptors.
GTP decreases the specific binding of [3H]flunitrazepam to cerebral cortical membrane sites. Scatchard analysis data show that GTP does not alter the maximum binding sites (Bmax), but increases the dissociation constant (KD) for [3H]flunitrazepam. GTP also inhibits the specific binding of the benzodiazepine antagonist [3H]CGS-8216. The specific binding of [3H]CGS-8216 displaceable by clonazepam is inhibited to a greater extent by GTP than that displaceable by the benzodiazepine antagonists, CGS-8216 or beta-CCE.
D000818:Animals; D001569:Benzodiazepines / Q000037:antagonists & inhibitors; D001667:Binding, Competitive / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D005445:Flunitrazepam / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D066298:In Vitro Techniques; D011720:Pyrazoles / Q000378:metabolism; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism; D011963:Receptors, GABA-A
D011720:Pyrazoles; D011955:Receptors, Drug; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D005445:Flunitrazepam; C033830:2-phenylpyrazolo(4,3-c)quinolin-3(5H)-one; D006160:Guanosine Triphosphate