title: Use of a cellular polyadenylation signal by viral transcripts in polyoma virus transformed cells.
abstract: The DNA sequences at and around the junctions between viral and cellular DNA in the polyoma virus transformed mouse cell line, TS-A-3T3, have been determined. No common sequence specificity or structural features at the joins have been observed. The sequence indicates that the 94K truncated large T antigen found in TS-A-3T3 cells is a hybrid protein in which the carboxy-terminal 19 amino acids are encoded by adjacent host sequences. Moreover, the three early region transcripts initiated in viral sequences are also hybrid in nature and appear to utilize a host polyadenylation signal associated with the hexanucleotide, AATAAA, found 100 bp beyond a viral-host join.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D000957:Antigens, Viral, Tumor; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D051379:Mice; D011061:Poly A / Q000235:genetics*; D011120:Polyomavirus / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D011061:Poly A; D004262:DNA Restriction Enzymes