Evidence against insulin synthesis BY IM-9 lymphocytes.
Insulin concentrated from acid/ethanol extracts of IMg lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.
D002457:Cell Extracts / Q000032:analysis; D002460:Cell Line; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000096:biosynthesis*; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism*; D011863:Radioimmunoassay; D012822:Silicon Dioxide
D002457:Cell Extracts; D007328:Insulin; C026679:octadecylsilica; D012822:Silicon Dioxide