Isolation of a trisaccharide containing 2-amino-2-deoxy-D-galacturonic acid from the Bordetella pertussis endotoxin.
4-O-(2-Amino-2-deoxy-alpha-D-glucopyranosyl-6-O-(2-amino-2-deoxy-alpha-D-galactopyranuronyl)-D-glucopyranose, a branched-chain trisaccharide, was isolated after hydrolysis of Bordetella pertussis endotoxin with 4 M HCl for 1 h at 100 degrees C. The trisaccharide was present in both polysaccharide moieties of the two constituent lipopolysaccharides of this endotoxin. Its structure was established by analysis of the 400-MHz nuclear magnetic resonance spectrum and by chemical and enzymatic degradation.
D001886:Bordetella pertussis / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004731:Endotoxins / Q000032:analysis*; D006603:Hexuronic Acids / Q000032:analysis*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification*; D014312:Trisaccharides / Q000032:analysis / Q000302:isolation & purification*; D014574:Uronic Acids / Q000032:analysis*
D004731:Endotoxins; D006603:Hexuronic Acids; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D014312:Trisaccharides; D014574:Uronic Acids; C011399:2-amino-2-deoxygalacturonic acid