title: Alignment of cloned amiE gene of Pseudomonas aeruginosa with the N-terminal sequence of amidase.
abstract: A restriction enzyme map was constructed for 5.1-kb fragment of Pseudomonas aeruginosa DNA inserted into plasmid pBR322. Restriction enzyme sites were matched to the N-terminal amino acid sequence of amidase to obtain alignment of the amiE gene within the cloned fragment.
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D010582:Bacteriophage lambda; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D005822:Genetic Vectors; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D000581:Amidohydrolases; C019665:amidase