title: Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.
abstract: DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the gyrA protein of Escherichia coli. No complementation was detected between the A subunit and the E. coli gyrB protein.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000302:isolation & purification* / Q000378:metabolism; D004278:DNA, Superhelical; D005816:Genetic Complementation Test; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight
chemical_list: D004278:DNA, Superhelical; D046911:Macromolecular Substances; D004250:DNA Topoisomerases, Type II