title: Constructions of gene libraries of Rana nigromaculata and Mus musculus with cosmid pHC79.
abstract: Gene libraries of Rana nigromaculata and Mus musculus (C57J6) have been constructed by the cosmid system developed by Collins using BamHl and Pstl as cloning restriction enzymes. A simplified formula, which is a modification of Clarke and Carbon derived from approximation, was used for calculating the number of colonies or recombinants necessary for the construction of a gene library. Pro+ and leu+ complementary to the recipient cells (HB101) with the genetic constitution of pro- and leu- have been selected from the BamH1 gene libraries of both Rana nigromaculata and the mouse in the present work.
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D005816:Genetic Complementation Test; D007930:Leucine / Q000235:genetics; D008433:Mathematics; D051379:Mice / Q000235:genetics*; D011392:Proline / Q000235:genetics; D011898:Ranidae / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D004274:DNA, Recombinant; D004247:DNA; D011392:Proline; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D007930:Leucine