Primary and secondary structure specificity of the cleavage of 'single-stranded' DNA by endonuclease Hinf I.
The interaction of endonuclease Hinf I with single-stranded fd DNA was examined. The sizes of the cleavage products indicate that the enzyme cuts this substrate at the same sequences as double-stranded DNA (GANTC). To determine whether or not the recognition sites in single-stranded DNA have to be present in double-stranded form in order to be cleaved, DNA fragments containing complementary or non-complementary Hinf I sequences were prepared and treated as substrates. The results suggest that completely base-paired recognition sites are necessary for cleavage. Sequences surrounding the Hinf I pentanucleotides significantly modulate the reaction rates.
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004277:DNA, Single-Stranded; D015252:Deoxyribonucleases, Type II Site-Specific; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases