Measurement of eicosapolyenoic acids in the serum by gas-liquid chromatography-chemical ionization mass spectrometry.
The use of octadeutero eicosatetraenoic acid as an internal standard for the reproducible measurement of serum eicosapolyenoic acids (C20:3, C20:4, and C20:5) by gas-liquid chromatography-chemical ionization mass spectrometry is described. The method has the following advantages. The physicochemical properties of the internal standard and the eicosapolyenoic acids are similar. The acids are easily separated from compounds of similar retention times by means of selected ion monitoring. The measurements can be made more rapidly, (10 min per sample) than with previous techniques.
D015126:8,11,14-Eicosatrienoic Acid / Q000032:analysis*; D001095:Arachidonic Acids / Q000032:analysis*; D015118:Eicosapentaenoic Acid; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D015118:Eicosapentaenoic Acid; D015126:8,11,14-Eicosatrienoic Acid