Anti-VPg antibody inhibition of the poliovirus replicase reaction and production of covalent complexes of VPg-related proteins and RNA.
Anti-VPg antibodies inhibited host-factor-dependent RNA synthesis by the poliovirus replicase but not oligo(U)-primed synthesis, implicating VPg in the de novo initiation of replicase products. Complexes of VPg-related polypeptide and newly made RNA could be immunoprecipitated by anti-VPg antibody from the host-factor-stimulated products of the replicase reaction. The complexes appeared to be covalently linked and involved 50 to 150 nucleotide chains of RNA that were RNAase-T1-resistant and could be largely poly(U).
D017955:Poliovirus / Q000235:genetics* / Q000276:immunology; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012367:RNA, Viral / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase / Q000276:immunology / Q000378:metabolism*; D014764:Viral Proteins / Q000276:immunology / Q000502:physiology*; D015678:Viral Structural Proteins; D014771:Virion / Q000502:physiology; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase