Amino acid sequence determination of the ADP,ATP carrier from beef heart mitochondria. The sequence of the C-terminal acidolytic fragment.
The primary structure of the C-terminal region (94 residues) of the ADP,ATP carrier of beef heart mitochondria is described. CNBr cleavage results in a large peptide (CB1) with Mr 22 000 and several small peptides (CB2 to CB8). Peptide separation was achieved by gel chromatography with 80% formic acid or with an ethanol/formic acid mixture. The amino acid sequence of the small CNBr peptides was determined by solid-phase techniques. Hydrolysis in formic acid cleaves the carrier protein into an Mr 23 000 fragment (A1) with the blocked N-terminus and an Mr 10 000 fragment (A2) starting with proline. The alignment of two CNBr fragments was possible by degradation of A2 by solid-phase methods for 34 steps. The remaining CNBr fragments were arranged by sequencing the tryptic peptides of citraconylated A2.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D005561:Formates; D008929:Mitochondria, Heart / Q000032:analysis*; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases; D010446:Peptide Fragments / Q000302:isolation & purification; D014357:Trypsin
D005561:Formates; D010446:Peptide Fragments; C030544:formic acid; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases; D014357:Trypsin; D003488:Cyanogen Bromide