title: A comparison of the genomes of polioviruses by cDNA:RNA hybridization.
abstract: RNAs from 11 strains of the three serotypes of poliovirus were isolated from infected HeLa cells and hybridized with representative, radioactive complementary DNA (cDNA) from either poliovirus type 1 or type 2. Hybridization and thermal denaturation were analysed by hydroxyapatite chromatography, cDNA to either poliovirus type 1 or 2 is competent to detect about 70 to 80% of the sequences in RNAs from heterotypic strains and more than 02% of the RNA sequences in homotypic strains. The three polio serotype genomes appear to have diverged from each other by about 20%. Both poliovirus type 1 and type 2 representative cDNA can be used for the detection of poliovirus genomic sequences in RNA isolated from tissue.
mesh_terms: D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D017955:Poliovirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA