Enzymatic transfer of ATP gamma S thiophosphate onto the 5'-hydroxyl of an oligonucleotide as a route to reactive oligonucleotide derivatives.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001483:Base Sequence; D003090:Coliphages / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism*; D013873:Thionucleotides / Q000378:metabolism*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D000469:Alkaline Phosphatase