Limited autolysis reduces the Ca2+ requirement of a smooth muscle Ca2+-activated protease.
Chicken gizzard smooth muscle contains large amounts of Ca2+-activated protease activity. Approximately 15 mg of purified enzyme can be obtained from 1 kg of fresh muscle. The enzyme consists of two subunits (Mr = 80,000 and 30,000) present in a 1:1 molar ratio. In the presence of CaCl2, the 80,000/30,000-dalton heterodimer (form I) is rapidly converted by limited autolysis to a 76,000/18,000-dalton species (form II). Both the 80,000- and 30,000-dalton subunits are degraded simultaneously. Moreover, the Ca2+ dependence for autolysis (K0.5 = 300 microM) is identical for both subunits. Neither the time course nor the Ca2+ dependence of the autolytic conversion reaction is altered by 10- and 20-fold molar excesses of substrate. Limited autolysis markedly reduces the Ca2+ requirement for substrate degradation. Using N-[ethyl-2-3H]maleimide-labeled 27,000-dalton cardiac myosin light chains as substrate, the Ca2+ requirement of form I was found to be quite high (K0.5 = 150 microM). Under similar conditions, the Ca2+ requirement of form II was 30-fold lower (K0.5 = 5 microM). Limited autolysis did not alter the specific activity of the enzyme. Our results demonstrate that smooth muscle contains an abundant amount of Ca2+-activated protease. Moreover, autolysis of this enzyme may play an important regulatory role by converting the native form to a species that is fully active at physiological levels of intracellular calcium ion.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002154:Calpain; D002645:Chickens; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D005895:Gizzard, Avian / Q000201:enzymology; D007700:Kinetics; D046911:Macromolecular Substances; D009130:Muscle, Smooth / Q000201:enzymology*; D013997:Time Factors
D046911:Macromolecular Substances; D010450:Endopeptidases; D002154:Calpain; D002118:Calcium