The effects of glutaraldehyde and osmium tetroxide on the erythrocyte membrane. A spin label study.
A nitroxide spin label probe technique was applied to study the interaction between glutaraldehyde or osmium tetroxide (OsO4) amd the membranes of horse erythrocytes, ghosts and liposomes prepared from erythrocyte lipids. Two major conclusions have been established: (1) Reaction of the fixation reagents with the membrane is selective. OsO4 reacts predominantly with lipids and glutaraldehyde with membrane proteins. (2) The lipid-protein interactions change after pretreatment by OsO4 or glutaraldehyde.
D000447:Aldehydes / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D009992:Osmium / Q000494:pharmacology*; D009993:Osmium Tetroxide / Q000494:pharmacology*
D000447:Aldehydes; D008081:Liposomes; D009992:Osmium; D009993:Osmium Tetroxide; D005976:Glutaral