Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains.
A study of the ability of 49 strains of FMD virus to replicate in BHK-21 monolayer cells maintained under a standard agar overlay containing 5.2 mM guanidine hydrochloride and to withstand heat inactivation at 54 degrees C for 1 h showed that strains belonging to serotypes C, O and Asia 1 were generally more resistant to guanidine and heat stable than the SAT 1, 2 and 3 serotypes. The type A viruses as a whole occupied an intermediate position between these two groups. In vitro passage in BHK-21 cells influenced the guanidine sensitivity of 3(O, C and SAT 3) of the 7 FMD serotypes suggesting that this is not a stable genetic marker. Heat stability of the FMD viruses, however, did not change on passage, suggesting that this is a stable characteristic inherent in any homogeneous FMD virus population.
D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D006224:Cricetinae; D005819:Genetic Markers; D006146:Guanidines / Q000494:pharmacology*; D006358:Hot Temperature; D007668:Kidney; D008647:Mesocricetus; D012703:Serotyping; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D005819:Genetic Markers; D006146:Guanidines