Induction of fructose 1,6-bisphosphatase and glucose 6-phosphatase in fetal mouse liver.
Fructose 1,6-bisphosphatase and glucose 6-phosphatase were induced in organ cultures of liver tissues from 15- and 19-day-old fetal mice, using a culture method that allowed the tissues to be maintained for 7 days in the absence of serum. In cultures from 15-day-old fetal liver, both enzyme activities increased significantly per milligram of DNA after a lag period of 1 to 3 days. In cultures from 19-day-old fetal liver only glucose 6-phosphatase increased in the absence of inducer. N6,O2'-Dibutyryladenosine 3',5'-monophosphate enhanced the rate of increase in fructose 1,6-bisphosphatase and glucose 6-phosphatase activities. The minimum effective concentration of the cyclic nucleotide was approximately 10(-6) M. Dexamethazone inhibited the increase in fructose 1,6-bisphosphatase during culture for 7 days. Glucose 6-phosphatase activity was enhanced by dexamethazone in cultures from 19-day-old fetal liver, but was without effect on glucose 6-phosphatase in cultures from 15-day-old fetal liver. The minimum inhibitory concentration of dexamethazone was less than 10(-8) M. The results suggest a complicated effect of the cyclic nucleotide on the two enzymes in fetal mouse liver as well as different mechanisms of action of dexamethazone on the induction of two enzymes.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005333:Fetus / Q000201:enzymology; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis*; D005952:Glucose-6-Phosphatase / Q000096:biosynthesis*; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D051379:Mice; D008813:Mice, Inbred ICR
D003994:Bucladesine; D003907:Dexamethasone; D004247:DNA; D006597:Fructose-Bisphosphatase; D005952:Glucose-6-Phosphatase