Distribution of prostaglandin E2 and prostaglandin F2 alpha receptors in human myometrium.
Human myometrium was studied for specific binding of PGE2 and PGF2 alpha. PGF2 alpha-binding was almost undetectable, but specific binding sites for PGE2 with high affinity (KD = 2.7 +/- 0.4 x 10(-9) M were demonstrated. The binding capacity for PGE2 exhibited a topically different distribution pattern with the highest values in the central parts and low to undetectable levels in the cervical region. Binding characteristics were analyzed by receptor kinetics, revealing a homogeneous receptor population. Binding capacity in uteri obtained from post-menopausal women was of the order of 900-940 fmol/mg protein. Oestrogen pre-treatment and pregnancy were associated with a 3-fold reduction of the PGE2-binding capacity.
D002462:Cell Membrane / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D006801:Humans; D008593:Menopause; D009215:Myometrium / Q000378:metabolism*; D011247:Pregnancy; D011458:Prostaglandins E / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects; D011982:Receptors, Prostaglandin / Q000187:drug effects; D014599:Uterus / Q000378:metabolism*
D011458:Prostaglandins E; D011460:Prostaglandins F; D011956:Receptors, Cell Surface; D011982:Receptors, Prostaglandin; D004958:Estradiol