title: Inverted repeats of Tn5 are transposable elements.
abstract: Experiments presented here show that each of the 1.5-kilobase inverted repeats of the kanamycin-resistance transposon Tn5 is transposable; we designate them IS50-L (left) and IS50-R (right). By DNA sequence analyses, IS50 is 1533 base pairs (bp) long and generates 9-bp direct repeats of target sequences. The ends of IS50 comprise a hyphenated 8-of-9-bp inverted repeat and are not used with equal efficiency; the outside ends are more active than the inside ends, suggesting that a strong transposase recognition site at the outside ends, suggesting that a strong transposase recognition site at the outside end extends beyond the 8 bp common to both ends.
mesh_terms: D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005838:Genotype; D007612:Kanamycin / Q000494:pharmacology; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D007612:Kanamycin; D004262:DNA Restriction Enzymes