title: Plasmid cloning vectors resistant to ampicillin and tetracycline which can replicate in both E. coli and Haemophilus cells.
abstract: We have constructed two plasmid vectors, pHCV5 and pHVTl, which will replicate both in Haemophilus and in Escherichia coli. Both contain the ampicillin-resistance gene and the replication origin from a Haemophilus plasmid, pRSF0885. Both also contain the pBR322 origin and therefore can be amplified in E. coli by chloramphenicol treatment. The plasmid pHCV5 contains the tetracycline-resistance gene of pBR322, and pHVT1 contains the analogous region from the transposon Tn10.
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006190:Haemophilus / Q000235:genetics*; D010403:Penicillin Resistance; D010957:Plasmids; D011815:R Factors; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D013752:Tetracycline