title: Nucleotide sequence of a Euglena gracilis chloroplast gene coding for the 16S rRNA: homologies to E. coli and Zea mays chloroplast 16S rRNA.
abstract: The nucleotide sequence of 16S rDNA from Euglena gracilis chloroplasts has been determined representing the first complete sequence of an algal chloroplast rRNA gene. The structural part of the 16S rRNA gene has 1491 nucleotides according to a comparative analysis of our sequencing results with the published 5'- and 3'-terminal "T1-oligonucleotides" from 16S rRNA from E. gracilis. Alignment with 16S rDNA from Zea mays chloroplasts and E. coli reveals 80 to 72% sequence homology, respectively. Two deletions of 9 and 23 nucleotides are found which are identical in size and position with deletions observed in 16S rDNA of maize and tobacco chloroplasts and which seem to be characteristic for all chloroplast rRNA species. We also find insertions and deletions in E. gracilis not seen in 16S rDNA of higher plant chloroplasts. The 16S rRNA sequence of E. gracilis chloroplasts can be folded by base pairing according to the general 16S rRNA secondary structure model.
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005056:Euglena gracilis / Q000235:genetics*; D005796:Genes; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010944:Plants / Q000032:analysis*; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity; D003313:Zea mays / Q000032:analysis
chemical_list: D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes