Cerulenin blocks fatty acid acylation of glycoproteins and inhibits vesicular stomatitis and Sindbis virus particle formation.
Cerulenin, an antibiotic that inhibits de novo fatty acid and cholesterol biosynthesis, effectively inhibited the formation and release of virus particles from chicken embryo fibroblasts infected with Sindbis or vesicular stomatitis virus (VSV). When added for 1 h at 3 h postinfection, the antibiotic blocked VSV particle production by 80 to 90% and inhibited incorporation of [3H]palmitic acid into the VSV glycoprotein by an equivalent amount. The effect of this antibiotic on virus protein and RNA biosynthesis was significantly less than that on fatty acid acylation. Nonacylated virus glycoproteins accumulated inside and on the surface of cerulenin-treated cells. These data indicate that fatty acid acylation is not essential for intracellular transport of these membrane proteins, but it may have an important role in the interaction of glycoproteins with membranes during virus assembly and budding.
D000085:Acetates / Q000378:metabolism*; D000215:Acylation; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology*; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D012845:Sindbis Virus / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D000085:Acetates; D000935:Antifungal Agents; D004279:DNA, Viral; D006023:Glycoproteins; D010169:Palmitic Acids; D014764:Viral Proteins; D002569:Cerulenin; D019308:Palmitic Acid; D003513:Cycloheximide