Experimental latent and recrudescent bovine herpesvirus-1 infections in calves.
Latent bovine herpesvirus-1 (BHV-1) infection was established in 6 calves and was demonstrated by reinduction of virus shedding after administration of corticosteroids. Latently infected calves failed to transmit BHV-1 during 4 weeks' contact with sentinel calves. Infected calves were killed and necropsied during latency or induced recrudescence. The BHV-1 DNA was demonstrated intranuclearly in trigeminal ganglion neurons by in situ hybridization. The BHV-1 antigen was demonstrated by immunofluorescence in trigeminal ganglion neurons during recrudescence. By electron microscopy, changes in the appearance of the Nissl bodies and a high frequency of nuclear bodies were observed in trigeminal ganglion neurons.
D000818:Animals; D002417:Cattle; D003907:Dexamethasone / Q000008:administration & dosage; D005455:Fluorescent Antibody Technique; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification*; D007241:Infectious Bovine Rhinotracheitis / Q000382:microbiology*; D008854:Microscopy, Electron; D009474:Neurons / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012668:Trigeminal Ganglion / Q000382:microbiology / Q000648:ultrastructure
D003907:Dexamethasone