Establishment and characterization of permanent cell lines from patients with acute and hairy cell leukemia.
Four cell lines were established from peripheral blood of patients with leukemia. All lines express the Epstein--Barr virus nuclear antigen (EBNA) and therefore should be classified as lymphoblastoid cell lines. However, one of the lines UHKT-5 established from a patient with acute myelomonocytic leukemia has some features not typical for lymphoblastoid cell lines. The cells resemble to macrophages with the phagocytic ability for yeasts, strong alpha-naphthylacetate esterase positivity in phagocytizing cells and unusually long villi. Another line UHKT-7 established from a patient with hairy cell leukemia expresses the same isotype of membrane immunoglobulins as the original hairy cells.
D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001772:Blood Cell Count; D002460:Cell Line; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000097:blood / Q000648:ultrastructure*; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000648:ultrastructure*; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000378:metabolism; D010587:Phagocytosis; D011951:Receptors, Complement / Q000032:analysis
D000956:Antigens, Viral; D011951:Receptors, Complement; D009283:Naphthol AS D Esterase