A cytochemical method for the demonstration of 5'-nucleotidase in mouse peritoneal macrophages, with cerium ions used as trapping agent.
A method was developed for the demonstration of 5'-nucleotidase in murine peritoneal resident macrophages. The cells are incubated cytochemically without agitation and cerium chloride is used as a trapping agent. Under these conditions, the great majority of the macrophages in the unstimulated peritoneal cavity show enzyme activity in the plasma membrane. In the presence of AMP-S (an AMP analogue inhibiting 5'-nucleotidase, as shown biochemically) there was a decrease in both the number of positive macrophages and the amount of reaction product on the plasma membranes. This indicates that the enzyme activity detected by our cytochemical procedure is attributable to 5'-nucleotidase.
D015720:5'-Nucleotidase; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002462:Cell Membrane / Q000201:enzymology; D002563:Cerium; D006651:Histocytochemistry / Q000379:methods; D008264:Macrophages / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism
D002563:Cerium; D000249:Adenosine Monophosphate; D010544:Peroxidases; D009708:Nucleotidases; D015720:5'-Nucleotidase; C026690:cerous chloride