Repair of psoralen-induced crosslinks in cells multiply infected with SV40.
Experiments were conducted to study the relationship between the production of interstrand crosslinks by 4,5',8-trimethylpsoralen (psoralen) in simian virus 40 DNA and the ability of psoralen to inactivate the virus. Under conditions where only single viral particles enter a given host cell, approximately one crosslink was lethal to the virus and could not be repaired. In contrast, when multiple viral genomes infected a host cell, psoralen-induced crosslinks were repaired (multiplicity reactivation). A model is proposed for multiplicity reactivation which involves genetic recombination between damaged viral genomes.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002522:Chlorocebus aethiops; D003432:Cross-Linking Reagents; D004260:DNA Repair; D004279:DNA, Viral / Q000378:metabolism*; D011564:Furocoumarins / Q000494:pharmacology*; D005814:Genes, Viral; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014307:Trioxsalen / Q000494:pharmacology*
D003432:Cross-Linking Reagents; D004279:DNA, Viral; D011564:Furocoumarins; D014307:Trioxsalen