title: Tumorigenicity of tsA and wild-type simian virus 40 transformed cells inoculated onto the chicken chorioallantoic membrane.
abstract: This study compares the tumorigenicity of SV40 primary tumor cell lines, tsA and wild-type SV40-transformed Chinese hamster cells, at two temperatures, 37 degrees C and 40.5 degrees C, inoculated onto the chorioallantoic membrane of the chicken egg. The SV40 primary tumor cell lines varied in their efficiency of takes at 37 degrees C from 78% for the H65-90B tumor line, 73% for the H80-7A and 25% for the H80-4 line. At 40.5 degrees C the H80-4 was unable to form tumors; however, the H80-7A and H80-4 produced 70% and 20% tumors respectively. Histologically, all CAM tumors were fibrosarcomas identical to the transplanted tumors, however, the tumor(s) at 40.5 degrees C were smaller. Chinese hamster wild-type SV40-transformed cells were equally efficient (32%) at tumor production at both temperatures. The tsA-SV40-transformed Chinese hamster cells (A58 and A58-2) induced 34% tumors at 37 degrees C and 9% tumors at 40.5 degrees C. At 37 degrees C these tumors were typical fibrosarcomas; however, the 40.5 degrees C tumors were smaller and less cellular, resembling a more differentiated fibrosarcoma. Therefore, the tsA Chinese hamster transformed cells were less efficient at tumor induction at the non-permissive temperature; however, the primary tumor lines also demonstrated a variability in tumorigenicity (H65-90B and H80-4). Possibly factors other than the temperature-sensitive viral protein (big "T" antigen) may be involved in establishing a tumor on the chicken CAM.
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D006224:Cricetinae; D003412:Cricetulus; D005321:Extraembryonic Membranes; D009154:Mutation; D009374:Neoplasms, Experimental / Q000209:etiology*; D013539:Simian virus 40 / Q000472:pathogenicity*; D013696:Temperature