title: [Specific activity and molecular forms of NAD-kinase in Neurospora crassa ontogeny].
abstract: The specific activity and molecular forms of NAD-kinase during ontogenesis of Neurospora crassa were investigated. The specific activity of the enzyme increased drastically at critical stages of fungal development, i.e. during conidia germination and during transition from the logarithmic to stationary growth stage, reaching 85 nmole NADP/hr/mg protein. By polyacrylamide gel electrophoresis four forms of NAD-kinase were revealed that had the following molecular masses: I-338,000, II-306,000, III-229,000, and IV-203,000. The vegetative mycelium contained predominantly form III, and conidia showed a high content of high-molecular-weight forms I and II.
mesh_terms: D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000254:growth & development; D010770:Phosphotransferases / Q000032:analysis / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor)
chemical_list: D007527:Isoenzymes; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase
vernacular_title: Udel'naia aktivnost' i molekuliarnye formy NAD-kinazy v ontogeneze Neurospora crassa.