Actin polymerization induced by chemotactic peptide and concanavalin A in rat neutrophils.
Changes in the state of actin in rat neutrophils were studied after chemotactic peptide and concanavalin A stimulation by using the DNase I inhibition assay. Actin polymerization occurred within seconds after stimulation with F-Met-Leu-Phe and concanavalin A. Pretreatment of cells with cytochalasin D prevented chemotactic peptide-induced actin polymerization. The addition of F-Met-Leu-Phe to lysed cells did not produce any change in actin state. These data offer strong evidence for receptor-induced actin polymerization and support the models implicating actin microfilament formation as a crucial event in cell activation. The observations on platelets, lymphocytes, neutrophils, and islets of Langerhans from different species suggest that actin polymerization might  be a universal intracellular event accompanying cell surface receptor perturbation in eukaryotic cells.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology*; D003599:Cytoskeleton / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives*; D009239:N-Formylmethionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000648:ultrastructure*; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects; D044042:Receptors, Formyl Peptide
D000199:Actins; D009842:Oligopeptides; D011956:Receptors, Cell Surface; D044042:Receptors, Formyl Peptide; D003208:Concanavalin A; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine