title: [Retrovirus activating potential of nuclear polyhedrosis viruses in mammalian cell cultures].
abstract: Activation of endogenous C-type retroviruses by nuclear polyhedrosis viruses (NPV) was investigated in "in vitro" cell culture systems of four mammalian species: mouse, rat, monkey and man. Cells were treated with NPV, (isolated from larvae and insect cell cultures), NPV-DNA, C-type virus-activating chemicals (halogenated pyrimidine analogue) and chemical insecticides alone and in combination. C-type retroviruses were activated only in mouse cells and transformed rat cells by the halogenated pyrimidine analogue. In NPV-treated cell cultures no C-type retrovirus activation was detectable. In simultaneous treatments of the cells with nuclear polyhedrosis viruses and chemicals no potentiating effects by the NPV could be detected. Virions of NPV in cell cultures showed unaltered morphology and upon reisolation were infectious in homologous insect cell cultures for a long time. No influence upon growth or morphology of the treated mammalian cells could be observed. From our experiments we conclude that the application of nuclear polyhedrosis viruses for pest control is uncritical with respect to their retrovirus activating potential in mammalian cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000882:Haplorhini; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D007304:Insect Viruses / Q000235:genetics / Q000502:physiology*; D007306:Insecticides / Q000494:pharmacology; D051379:Mice; D051381:Rats; D012190:Retroviridae / Q000254:growth & development*; D014162:Transfection; D014775:Virus Activation
chemical_list: D007306:Insecticides; D007065:Idoxuridine; D004121:Dimethyl Sulfoxide
vernacular_title: Untersuchungen über das Retrovirus aktivierende Potential von Kernpolyederviren in Säugetier-Zellkulturen.