Organization of herpes simplex virus type 1 deoxyribonucleic acid during replication probed in living cells with 4,5',8-trimethylpsoralen.
The structure of herpes simplex virus type 1 (HSV-1) DNA in the nuclei of living infected cells was studied with the DNA photoaffinity probe 4,5',8-trimethylpsoralen. The rate of photobinding to HSV-1 DNA was compared to that of a suitable internal control at different times during infection. The rates of photobinding to DNA packaged in virions, capsids, and prereplicative and postreplicative DNA were characteristically different. By 4 h after infection, after the initiation of DNA replication, the rate of photobinding to HSV-1 DNA increased 4 times relative to the rate of binding to the host DNA. The enhanced rate of photobinding to HSV-1 DNA was maintained at all later times during infection and was not affected when frequent single-strand breaks were introduced in HSV-1 DNA by gamma irradiation of infected cells. The results suggest that the bulk of the replicating herpes DNA is free of torsional tension and that the differing rates of photobinding are attributable to changes in accessibility of the HSV-1 DNA. The results are compatible with previous proposals, based on in vitro studies, that intranuclear HSV-1 DNA is primarily free of nucleosomal organization and suggest that there are few, if any, unrestrained DNA supercoils averaged over the entire HSV-1 genome.
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D002213:Capsid / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism* / Q000528:radiation effects; D011564:Furocoumarins / Q000378:metabolism; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008027:Light; D018139:Simplexvirus; D014307:Trioxsalen / Q000378:metabolism / Q000528:radiation effects; D014771:Virion / Q000378:metabolism
D000345:Affinity Labels; D004279:DNA, Viral; D011564:Furocoumarins; D014307:Trioxsalen