Production of hepatitis B e antibody in Epstein-Barr virus-induced B lymphocyte cell lines.
B lymphocytes separated from anti-HBe-positive donors were established as lymphoblastoid cell lines by infection with EBV, but anti-HBe in the culture supernatant from such lymphoblastoid cell lines could not be detected. The lymphoblastoid cell lines were rosetted with HBe antigen-coupled SRBC to prepare cells for the production of specific anti-HBe. Antibody activity in the culture supernatant against rosette forming cells was detected by RIA, ID, and R-PHI tests during the first 1 to 4 wk, but not after 5 wk. The activity in the supernatant was not destroyed by treatment with 2-mercaptoethanol, indicating that the antibody might be IgG.
D000914:Antibodies, Viral / Q000096:biosynthesis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006511:Hepatitis B Antigens / Q000276:immunology*; D006513:Hepatitis B e Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008623:Mercaptoethanol / Q000494:pharmacology; D012397:Rosette Formation
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D008623:Mercaptoethanol