Biological properties of a calcium-modulator protein from Phycomyces blakesleeanus.
1. A modulator protein having properties similar to calmodulin-like proteins could be detected in Phycomyces the first day of growth. 2. The modulator protein from Phycomyces was partially purified to a specific activity of 9,000 Units/mg protein. 3. The modulator protein was similar to calmodulin with respect to: (a) the ability to stimulate a calcium-plus-calmodulin-dependent cAMP phosphodiesterase from bovine brain; (b) the inhibitory effect of trifluoperazine in this process; (c) the stimulation of the [Ca2+ + Mg2+)-ATPase from human erythrocyte membranes. 4. Maximum effect of modulator protein in the above mentioned reactions was achieved at micromolar calcium concentrations.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000097:blood; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology; D005658:Fungi / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010800:Phycomyces / Q000378:metabolism*; D014268:Trifluoperazine / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D014268:Trifluoperazine; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000251:Adenosine Triphosphatases; D002118:Calcium