title: Interactions of dicyclohexylcarbodiimide with myelin proteolipid.
abstract: Dicyclohexylcarbodiimide (DCCD) is known to bind preferentially to a proteolipid subunit of proton-translocating systems and thereby to inhibit proton transport. In the present study we show that, in an aqueous medium, DCCD binds to the bovine white matter proteolipid apoprotein, the major protein of central nervous system myelin. The binding is dependent on time, temperature, and concentration and is not inhibited by the hydrophilic carbodiimide 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. By contrast, when the incubation is carried out in chloroform/methanol no labeling by DCCD is demonstrable. In isolated rat myelin, DCCD binds specifically to the proteolipid and not to the myelin basic proteins. Liposomes reconstituted with the myelin proteolipid apoprotein transport protons, as assayed by quenching of the fluorescence of 9-aminoacridine. Preincubation of proteolipid-containing liposomes with DCCD results in an inhibition of transport. These studies have important implications for a possible ionophoric function of the myelin proteolipid and for the occurrence of transport processes within myelin.
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002234:Carbodiimides / Q000494:pharmacology*; D002417:Cattle; D004024:Dicyclohexylcarbodiimide / Q000378:metabolism / Q000494:pharmacology*; D008074:Lipoproteins / Q000502:physiology*; D009185:Myelin Proteins / Q000502:physiology*; D009186:Myelin Sheath / Q000187:drug effects*; D011522:Protons
chemical_list: D001059:Apoproteins; D002234:Carbodiimides; D008074:Lipoproteins; D009185:Myelin Proteins; D011522:Protons; D004024:Dicyclohexylcarbodiimide