title: [Interrelationship of polyphosphate metabolism and levorin biosynthesis in Streptomyces levoris].
abstract: The effect of inorganic phosphate on biosynthesis of the polyene antibiotic levorin by Streptomyces levoris was studied. At phosphate concentration of 4.0 mM levorin biosynthesis is repressed by 90%, resulting in an increase of ATP and a condensed inorganic polyphosphates content in the producer cells. At phosphate concentration optimal for levorin production (0.04 mM) the level of intracellular ATP sharply falls by the beginning of the steady-state phase of the producer growth and that of polyphosphates decreases 3-6-fold. The inorganic phosphate exerts different effects on polyphosphate metabolism enzymes, such as polyphosphate: D-glucose-6-phosphotransferase, polyphosphate phosphohydrolase, tripolyphosphate phosphohydrolase, pyrophosphate phosphohydrolase, alkaline and acid phosphatase. The strongest effect of phosphate excess is observed in the case of polyphosphate: D-glucose-6-phosphotransferase, whose activity decreases 2-5-fold. The activity of this enzyme was shown to be correlated with the antibiotic accumulation. The data obtained are indicative of interrelationship between the polyphosphate metabolism and levorin biosynthesis.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000935:Antifungal Agents / Q000096:biosynthesis*; D002174:Candicidin / Q000096:biosynthesis*; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011122:Polyphosphates / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D010710:Phosphates; D011122:Polyphosphates; D002174:Candicidin; D000255:Adenosine Triphosphate; D010744:Phosphoric Monoester Hydrolases
vernacular_title: O vzaimosviazi polifosfatnogo obmena s biosintezom levorina i Streptomyces levoris.