Isopycnic separation of Escherichia coli cultures possessing colonization factor antigen I.
A culture of Escherichia coli possessing colonization factor antigen I was subjected to isopycnic separation on Percoll gradients. The results demonstrated successful division of the culture into two populations: (i) bacteria which cause mannose-resistant hemagglutination and (ii) bacteria which lack the ability to hemagglutinate in the presence of mannose.
D000942:Antigens, Bacterial / Q000032:analysis*; D002500:Centrifugation, Isopycnic; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification*; D036781:Fimbriae Proteins; D011205:Povidone; D012822:Silicon Dioxide
D000942:Antigens, Bacterial; C022924:colonization factor antigens; D036781:Fimbriae Proteins; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone