Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
A rat monoclonal antibody against the murine transferrin receptor has been identified. The receptor is a 95,000 molecular weight species that exists in the cell membrane as a disulphide-bonded dimer. Whereas 29 of 29 murine hematopoietic tumor cell lines express detectable numbers of transferrin receptors, less than 1% of adult thymocytes or spleen cells and only 5% of bone marrow cells are positive. However, fetal liver and neonatal spleen contain substantial numbers of transferrin receptor-positive cells. Induction of Friend cells in vitro with dimethyl-sulphoxide leads to an overall increase in the expression of transferrin receptors on the cell surface. The anti-transferrin receptor antibody we have obtained partially blocks iron uptake from 59Fe-transferrin by a variety of murine cell lines and inhibits the growth of a murine myeloma cell line in vitro.
D000818:Animals; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000032:analysis; D002460:Cell Line; D007501:Iron / Q000378:metabolism; D007938:Leukemia / Q000032:analysis; D008099:Liver / Q000032:analysis; D008223:Lymphoma / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D011990:Receptors, Transferrin; D013154:Spleen / Q000032:analysis; D013601:T-Lymphocytes / Q000032:analysis; D014168:Transferrin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron