Solubilization of the glycine receptor from rat spinal cord.
Glycine receptors, as detected by glycine-displaceable [3H]strychnine binding, were solubilized from a membrane fraction of rat spinal cord by the non-ionic detergent Triton X-100. The solubilized material retained its high affinity for [3H]strychnine and exhibited the typical pharmacological properties of the membrane-bound glycine receptor. On sucrose density gradients, the solubilized receptor had a sedimentation coefficient of 8.3 +/- 0.4 S. Gel exclusion chromatography on Sepharose 6 B in the presence of phosphatidylcholine gave a Stokes radius of 7.3 +/- 0.3 nm.
D000818:Animals; D005998:Glycine / Q000378:metabolism*; D008526:Medulla Oblongata / Q000378:metabolism; D011149:Pons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012995:Solubility; D013116:Spinal Cord / Q000378:metabolism*; D013331:Strychnine / Q000378:metabolism
D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D013331:Strychnine; D005998:Glycine