title: Insulin and dexamethasone effects on liver fructose bisphosphatase.
abstract: Insuling perfusion of isolated diabetic rabbit liver and glucocorticoid perfusion of isolated normal rabbit liver resulted in an increased accumulation of immunoreactive fructose bisphosphatase (nmol/g). The insulin effect was maximal at 30 min with concentration (nmol/g) and specific activity (units/nmol) returning to normal within 2 h after insulin removal. Similarly, dexamethasone perfusion increased enzyme concentration and decreased its specific activity; however, the effects were maximal at 60-120 min. The results parallel those previously demonstrated in whole animals and establish that both hormones influence the enzyme by direct effects on the liver.
mesh_terms: D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D010477:Perfusion; D011817:Rabbits; D013997:Time Factors
chemical_list: D007328:Insulin; D003907:Dexamethasone; D006597:Fructose-Bisphosphatase