Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the beta subunit gene of the rifampicin resistant rpoB255 mutant.
The transducing phage lambda dsupM814 and the plasmid pIB1830 containing the wild-type rpoB gene have been constructed and the primary structure of the gene's central fragment has been established. In contrast with the wild-type, the gene of the rpoB255 mutant, whose primary structure has been published, was found to contain an A.T. leads to T.A. transversion entailing the substitution of a valine residue for the aspartic acid residue (516) of the wild-type beta subunit.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005796:Genes / Q000187:drug effects; D009154:Mutation; D010957:Plasmids; D012293:Rifampin / Q000494:pharmacology*; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D012293:Rifampin