title: Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
abstract: We have identified the single phosphorylated tyrosine in p60src, the transforming protein of Rous sarcoma virus, as part of the sequence. NH2-Arg-Leu-Ile-Glu-Asp-Asn-Glu-Tyr(P)-Thr-Ala-Arg-COOH. Therefore, this is a sequence that is recognized efficiently by a tyrosine protein kinase in vivo. Phosphorylation of tyrosine in cellular proteins appears to play a role in malignant transformation by four classes of genetically distinct RNA tumor viruses. Phosphorylated tyrosines in several other proteins resemble of the tyrosine in p60src in that they are located 7 residues to the COOH-terminal side of a basic amino acid and either 4 residues to the COOH-terminal side of, or in close proximity to, a glutamic acid residue. Therefore it is possible that these features play a role in the selection of sites of phosphorylation by some tyrosine protein kinases. However, several clear exceptions to this rule exist.
mesh_terms: D000011:Abelson murine leukemia virus; D001355:Alpharetrovirus; D000595:Amino Acid Sequence; D001358:Avian Sarcoma Viruses; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D007143:Immunoglobulin Heavy Chains; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D015291:Transforming Growth Factors; D014443:Tyrosine / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D007143:Immunoglobulin Heavy Chains; D010455:Peptides; D014764:Viral Proteins; D014443:Tyrosine; D015291:Transforming Growth Factors; D011494:Protein Kinases