Detergent effects of kinetic properties of (Na+ +K+)-ATPase from kidney membranes.
Studies on (Na+ +K+)-ATPase generally employ detergents such as SDS and deoxycholate. Under such conditions, the purified enzyme possesses high specific activity. The (Na+ +K+)-ATPase from kidney membranes was unmasked by deoxycholate and SDS as described by Jłrgensen and its kinetic properties were studied. The results suggest that these detergents induce some irreversible alterations in the kinetic properties of the native enzyme. Another detergent, saponin, unmasked the (Na+ +K+)-ATPase as effectively as did SDS, but it seems to affect the kinetic properties of the native enzyme to a lesser extent.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D007679:Kidney Medulla / Q000201:enzymology*; D007700:Kinetics; D010042:Ouabain / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D010710:Phosphates; D012503:Saponins; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium