Uninfected cell polymerase efficiently transcribes early but not late herpes simplex virus type 1 mRNA.
The sequences of the DNAs encoding the 5' ends of one early and one late herpes simplex virus type 1 mRNA were analyzed, and the 5' ends of these mRNA species were precisely located. Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstream from their respective 5' ends. The early mRNA was efficiently transcribed by a commercially available uninfected cell lysate system, but the late mRNA was not. This difference between early and late mRNAs appears to be general in this virus.
D001483:Base Sequence; D002472:Cell Transformation, Viral; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D012333:RNA, Messenger / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes