An in vitro assay for quantifying the virus of avian encephalomyelitis.
Chicken embryo brain (CEB) cell cultures support the replication of embryo-adapted strains, vaccine strains, and field isolates of avian encephalomyelitis virus. A centrifugal force of 1,500 X g was applied during virus adsorption. Viral antigen was detected in the infected cells by using the indirect fluorescent-antibody technique (IFAT). Combining the infectivity of the virus in CEB cell culture with the ability to detect viral antigen by the IFAT resulted in the development of a virus-titration method. This in vitro assay proved to be more sensitive than the standard embryo-inoculation assay. It was concluded that the in vitro assay provides a satisfactory alternative to the embryo-inoculation assay.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain; D002642:Chick Embryo / Q000382:microbiology; D046508:Culture Techniques; D001350:Encephalomyelitis Virus, Avian / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D004770:Enterovirus / Q000254:growth & development*; D005455:Fluorescent Antibody Technique; D008828:Microbiological Techniques; D014779:Virus Replication
D000956:Antigens, Viral