Immunohistochemical and biochemical studies on collagen types in angiofibromas and shagreen patches from patients with tuberous sclerosis.
The distribution of collagen types in angiofibroma and shagreen patch from patients with tuberous sclerosis was examined biochemically and immunohistochemically by indirect immunofluorescence microscopy using type-specific anti-collagen antibodies. Types I and III collagens were distributed in the interstitium of whole tumor tissues of angiofibriomas and a shagreen patch, showing relatively fine fibers at the periphery and large bundles in the center. No significant difference in the distribution of type I and III collagens was observed. Densitometric analysis of gel electrophoresis patterns of collagens solubilized from the skin by limited pepsin digestion revealed that both type I and III collagens were the main constituents of the diseased regions of the skin with tuberous sclerosis, and the ratio of type I collagen/type III collagen was relatively constant, which was not contradictory to the results of immunofluorescence microscopy. The presence of an unidentified component, probably type V collagen, was observed in the angiofibroma tissue from one patient with tuberous sclerosis.
D000293:Adolescent; D000328:Adult; D003094:Collagen / Q000378:metabolism*; D003238:Connective Tissue / Q000473:pathology; D005153:Facial Neoplasms / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D018219:Histiocytoma, Benign Fibrous / Q000473:pathology*; D006801:Humans; D009506:Nevus / Q000473:pathology*; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology*; D014402:Tuberous Sclerosis / Q000473:pathology*
D003094:Collagen