NADPH-dependent reduction of cytochrome P-450 in liver microsomes from vitamin C-deficient guinea pigs: effect of benzphetamine.
The effect of dietary vitamin C on the reduction of cytochrome c and cytochrome P-450 by NADPH-cytochrome P-450 reductase was determined in guinea pig liver microsomes. Acute vitamin C deficiency decreased hepatic microsomal cytochrome P-450 content 21% and the rate of cytochrome P-450 reduction 66% without affecting cytochrome c reduction. However, the vitamin status of the animals did not affect the enhancement in cytochrome P-450 reduction produced by the addition of a type I substrate to the reaction mixture. The results suggest that vitamin C deficiency selectivity affects the transfer of electron from NADPH to cytochrome P-450 and that this effect does not result from a change in the spin state of cytochrome P-450.
D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use; D001206:Ascorbic Acid Deficiency / Q000188:drug therapy / Q000378:metabolism*; D001589:Benzphetamine / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010627:Phenethylamines / Q000494:pharmacology*
D003574:Cytochrome c Group; D010627:Phenethylamines; D001589:Benzphetamine; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D001205:Ascorbic Acid