title: Establishment and characterization of permanent cell lines from patients with acute and hairy cell leukemia.
abstract: Four cell lines were established from peripheral blood of patients with leukemia. All lines express the Epstein--Barr virus nuclear antigen (EBNA) and therefore should be classified as lymphoblastoid cell lines. However, one of the lines UHKT-5 established from a patient with acute myelomonocytic leukemia has some features not typical for lymphoblastoid cell lines. The cells resemble to macrophages with the phagocytic ability for yeasts, strong alpha-naphthylacetate esterase positivity in phagocytizing cells and unusually long villi. Another line UHKT-7 established from a patient with hairy cell leukemia expresses the same isotype of membrane immunoglobulins as the original hairy cells.
mesh_terms: D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001772:Blood Cell Count; D002460:Cell Line; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000097:blood / Q000648:ultrastructure*; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000648:ultrastructure*; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000378:metabolism; D010587:Phagocytosis; D011951:Receptors, Complement / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D011951:Receptors, Complement; D009283:Naphthol AS D Esterase