NAD glycohydrolase activity in the liver of rats fed on excess leucine diet and low or high protein diet.
In rats fed for 2 weeks on a 10% casein diet supplemented with 5% leucine, the hepatic NAD content was lowered when comparing with the control rats pair-fed on the 10% casein diet. The hepatic NAD glycohydrolase [EC 3.2.2.5] activity increased by 25% in the leucine-supplemented diet-fed rats and by 40% in the nicotinic acid-free diet-fed rats. The hepatic NAD glycohydrolase activity in the rats fed on the nonprotein diet freely for 1 week was elevated by 60% compared to the rats fed on the 18% casein diet. The hepatic NAD content in the former animals was significantly lower than that in the latter. The 70% casein diet caused changes in neither hepatic NAD content nor NAD glycohydrolase activity. These results indicated an inverse relationship between hepatic NAD content and NAD glycohydrolase activity.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004044:Dietary Proteins / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D007930:Leucine / Q000009:adverse effects*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009525:Niacin; D009536:Niacinamide / Q000031:analogs & derivatives / Q000378:metabolism; D009539:Nicotinic Acids / Q000172:deficiency; D051381:Rats; D011919:Rats, Inbred Strains
D004044:Dietary Proteins; D009539:Nicotinic Acids; D009243:NAD; D009536:Niacinamide; D009525:Niacin; D009244:NAD+ Nucleosidase; D007930:Leucine; C024058:N(1)-methylnicotinamide