Confirmation of a tetrahydrocannabinol metabolite in urine by gas chromatography.
A new method for confirming urinary 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid, the major metabolite of tetrahydrocannabinol, has been developed. The metabolite is extracted, derivatized to the methyl ester, methyl ether, and analyzed on a gas chromatograph equipped with a flame ionization detector. In spiked urine specimens, metabolite concentrations as low as 20 ng/mL have been detected by this procedure. In a random sampling of urines, greater than 95% correlation was obtained between confirmation by this method and confirmation by gas chromatography/mass spectrometry in those specimens producing an immunological response greater than 75 ng/mL.
D002849:Chromatography, Gas; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007124:Immunoenzyme Techniques
C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; D013759:Dronabinol