Combined effect of acyclovir and amphotericin B on the replication of pseudorabies virus in BHK-21 cells.
Acyclovir, known as an antiherpetic agent, showed an inhibitory effect on the propagation of pseudorabies virus in BHK-21 cells. The antiviral effect of acyclovir was observed by plaque reduction, as well as by the inhibition of the virus-stimulated uptake of thymidine by BHK-21 cells. Amphotericin B potentiated the antiviral activity of acyclovir. The optimal concentrations of polyene antibiotic expressing the potentiating effect were lower than required for the induction of K+ leakage from the cells. There was no evident amphotericin B-induced stimulation of thymidine incorporation into infected BHK-21 cells. The model presented may be useful to study the potentiation phenomenon of polyene macrolide antibiotics.
D000212:Acyclovir / Q000494:pharmacology*; D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D006224:Cricetinae; D004357:Drug Synergism; D011558:Herpesvirus 1, Suid / Q000187:drug effects*; D007668:Kidney / Q000382:microbiology; D013936:Thymidine / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D000666:Amphotericin B; D013936:Thymidine; D000212:Acyclovir