title: Ligation of restriction endonuclease-generated DNA fragments using immobilized T4 DNA ligase.
mesh_terms: D010582:Bacteriophage lambda / Q000032:analysis; D003080:Cold Temperature; D011088:DNA Ligases / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D010957:Plasmids; D011118:Polynucleotide Ligases / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology*
chemical_list: D004279:DNA, Viral; D004800:Enzymes, Immobilized; D004262:DNA Restriction Enzymes; D011088:DNA Ligases; D011118:Polynucleotide Ligases