title: Effect of ethanol administration and withdrawal on benzodiazepine receptor binding in the rat brain.
abstract: Administration of single doses of ethanol or presence of ethanol in the incubation medium does not change 3H-flunitrazepam binding in rat cerebral cortex, hippocampus and cerebellum. In rats made dependent on ethanol by three daily ethanol administrations for six days and sacrifices 1 hr after the last ethanol dose 3H-flunitrazepam binding is decreased in the cerebral cortex and cerebellum. This decrease is present only in membranes preincubated with Triton X-100 and is due to decreased number of binding sites. In rats sacrificed 16 hours after the last chronic ethanol dose the 3H-flunitrazepam binding returns to control levels.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000009:adverse effects*; D005445:Flunitrazepam / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism; D011963:Receptors, GABA-A; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D011955:Receptors, Drug; D011963:Receptors, GABA-A; D000431:Ethanol; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam