Isolation and preliminary characterization of temperature-sensitive mutants of poliovirus type 1.
We isolated six temperature-sensitive mutants of poliovirus type 1 (Mahoney) by hydroxylamine mutagenesis and replica plating at 31, 33 (permissive), and 39 degrees C (restrictive). One of these mutants, designated tsB9, was chosen for more detailed examination. tsB9 accumulated 25% of the wild-type amount of virus-specific RNA at the restrictive temperature. We found that tsB9 was not able to synthesize mature, 35S single-stranded RNA at the restrictive temperature. In spite of the absence of significant RNA synthesis, tsB9 retained the ability to inhibit host protein synthesis during infection at 39 degrees C at about the same rate as wild-type virus.
D019811:Hydroxylamine; D006898:Hydroxylamines; D009154:Mutation; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D014779:Virus Replication
D006898:Hydroxylamines; D012367:RNA, Viral; D019811:Hydroxylamine