Characterization of heavy particles of adeno-associated virus type 1.
The temperature-sensitive mutant ts4 of adenovirus type 2 (Ad-2) is capable of complementing adeno-associated virus type 1 (AAV-1) in HEp2, KB and HEK cells at 34 degrees C and 39 degrees C when used as a helper virus. Heavy non-infectious AAV-1 particles can be generated by using the mutant ts4 in HEp2 cells. When AAV-1 is grown in serial passages in HEp2 cells, both the wild-type Ad-2 and the mutant ts4 give rise to heavy, less infectious AAV-1 particles. The heavy AAV-1 particles generated by Ad-2 in advanced serial passages retain the property of having CF and IF antigens, but the AAV-1 generated by the mutant in advanced serial passages lose this property. There is no appreciable difference in the particle counts made by electron microscopy of AAV-1 preparations generated either by Ad-2 or the mutant ts4. Analysis by polyacrylamide gel electrophoresis of purified heavy AAV generated by ts4 indicates that in late passage an additional polypeptide of higher mol. wt. than the three structural polypeptides is detected.
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D000229:Dependovirus / Q000648:ultrastructure*; D005816:Genetic Complementation Test; D006378:Helper Viruses / Q000235:genetics; D006801:Humans; D009154:Mutation; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000648:ultrastructure
D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins