Effects of ACTH and alprenolol treatments on muscle and brown fat enzyme activities and weights in the rat.
Chronic ACTH treatment (3 IU/kg daily for 8 days) caused an increase in wet weight of the interscapular brown adipose tissue (ISBAT) in the rat without affecting the enzyme activities of the cardiac or skeletal muscles or of ISBAT. On the other hand, chronic beta-blockade (alprenolol 10 mg/kg daily for 8 days) induced elevated activities in the oxidative enzymes of the ISBAT but not of the muscle tissues measured. Combined ACTH and alprenolol treatment also increased significantly the citrate synthase and malate dehydrogenase activities and protein concentration, but decreased the weight of ISBAT to normal. The results suggest that, although a direct antagonism between the beta-blockers and ACTH has not been demonstrated, beta-blockers can abolish the ACTH-induced weight gain of the ISBAT.
D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000526:Alprenolol / Q000494:pharmacology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002950:Citrate (si)-Synthase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000526:Alprenolol; D000324:Adrenocorticotropic Hormone; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D002950:Citrate (si)-Synthase