title: Detection of herpes simplex virus type 2 glycoproteins expressed in virus-transformed rat cells.
abstract: Rat embryo fibroblasts transformed by herpes simplex virus type 2 (HSV-2) were assayed for the expression of certain virus-specific glycoproteins on the surface membranes. Monospecific antisera to HSV-2-specific glycoproteins, designated gAgB, gC, and gX, were used in membrane immunofluorescence studies with HSV-2-transformed cell lines tREF-G-1, tREF-G-2, and a tumor-derived rat fibrosarcoma cells line produced in syngeneic rats inoculated with tREF-G-1 cells. Analysis of the three HSV-2-transformed cell lines showed that antisera to the gAgB and gX glycoproteins were reactive with these cells. In contrast, no significant reactivity was observed when anti-gC serum was reacted with the HSV-2-transformed cell lines. All three antiglycoprotein sera reacted positively with rat cells productively infected with HSV-2. Additionally, the HSV-2-transformed and tumor-derived cell lines showed positive internal immunofluorescence after reaction with antiserum to an early, nonstructural viral protein designated VP143 (molecular weight, 143,000). Infectivity of HSV-2 in standard plaque assays was neutralized by hyperimmune rat antisera to tREF-G-2 or rat fibrosarcoma cells and to HSV-2 virions and by sera from rats bearing the fibrosarcoma. Adsorption of rat-anti-HSV-2 serum with tREF-G-2 or rat fibrosarcoma cells reduced neutralizing activity to 10 and 12%, respectively, compared with 90% neutralization by antiserum adsorbed with nontransformed rat embryo fibroblast cells and 100% neutralization with unadsorbed antiserum. In summary, HSV-2-transformed rat cells retained and expressed genetic information necessary for the production of HSV-2 glycoproteins and a nonstructural protein after high passage in tissue culture or in the syngeneic host.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000276:immunology; D005354:Fibrosarcoma / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D051381:Rats; D011915:Rats, Inbred BUF; D018139:Simplexvirus / Q000276:immunology*; D014764:Viral Proteins
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins