cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.
A recombinant plasmid with a cDNA sequence transcribed from mouse skeletal muscle RNA is shown to hybridize with mRNAs for myosin light chains LC1F and LC3F. The inserted fragment corresponds exclusively to the 3'-noncoding region of the mRNA. It hybridizes almost exclusively with the two light chain messengers from fast skeletal muscle RNA of adult mouse. Slight hybridization is seen with RNA from heart muscle and embryonic skeletal muscle. The implications of the conservation of the 3'-noncoding regions between the two mRNAs are discussed.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D051379:Mice; D009132:Muscles / Q000378:metabolism; D015879:Myosin Subfragments; D009218:Myosins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*
D015879:Myosin Subfragments; D010446:Peptide Fragments; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D009218:Myosins