Human neutrophils transform prostaglandins by a myeloperoxidase-dependent mechanism.
Intact human neutrophils, incubated with the soluble stimulant phorbol myristate acetate, discharge lysosomal components, generate oxygen metabolites, and transform exogenous 6-keto-prostaglandin F1 alpha, prostaglandin E2, and prostaglandin F2 alpha as assessed by thin layer radiochromatography. Neutrophils alone were incapable of transforming the prostaglandins. The addition of catalase or the myeloperoxidase inhibitor, azide, protected all three prostaglandins from the phorbol-stimulated neutrophils. Neither superoxide dismutase, heat-inactivated catalase, nor albumin had any inhibitory effect in this system. A model system consisting of glucose-glucose oxidase, as a source of H2O2, purified myeloperoxidase, and chloride was also able to transform the prostaglandins in an identical fashion. Neither glucose-glucose oxidase alone nor glucose-glucose oxidase and myeloperoxidase under chloride-free conditions were able to mediate this transformation. Thus, it appears that intact human neutrophils can transform prostaglandins by a mechanism dependent on H2O2, the lysosomal enzyme myeloperoxidase, and chloride. Given the importance of prostaglandins in regulating immune function, neutrophil-dependent prostaglandin transformation could play a novel role in modulating the inflammatory response.
D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D009504:Neutrophils / Q000201:enzymology* / Q000378:metabolism; D009195:Peroxidase / Q000097:blood*; D010544:Peroxidases / Q000097:blood*; D011453:Prostaglandins / Q000097:blood*; D013482:Superoxide Dismutase / Q000494:pharmacology
D001386:Azides; D011453:Prostaglandins; D010544:Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase