Reduced Na+, K+ -ATPase activity in intact red cells and isolated membranes from obese man.
Na+, K+ -ATPase activity was measured in red blood cells from 20 nondiabetic euthyroid male Pima Indians with varying degrees of obesity; their body mass indices ranged from 22-60 kg/m2. The na+, K+ -ATPase, measured both by 86Rb uptake in intact cells and ATP hydrolysis by purified membranes, was inversely correlated with body mass index (r = -0.62; P less than 0.005 and r = -0.75; P less than 0.0001, respectively). These results confirm that obesity is associated with decreased Na+, K+ -ATPase in intact red blood cells, and provide the first demonstration of a reduced sodium pump in isolated red cell membrane preparations from obese men.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D001130:Arizona; D001823:Body Composition; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007198:Indians, North American; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D008875:Middle Aged; D009765:Obesity / Q000201:enzymology*; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D008565:Membrane Proteins; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium