Na+/K+ ATPase and cell growth--III: Enzymatic activity in cultured and EGF stimulated HeLa cells.
1. The behaviour of Na+/K+ ATPase during the growth of ectodermal tumoral cells (HeLa) has been investigated. 2. Besides spontaneously growing cells, also samples stimulated with EGF have been tested. 3. The content of--SH groups in the homogenates of both the untreated and the EGF stimulated samples has been tested. 4. Results show a decrease of enzymatic activity during the culture of neoplastic cells and an enhancement of this behaviour in the EGF stimulated cells due to the action of the hormone on--SH groups.
D002455:Cell Division; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000032:analysis
D013438:Sulfhydryl Compounds; D004815:Epidermal Growth Factor; D000254:Sodium-Potassium-Exchanging ATPase