Poly(ADP-ribose) synthesis in human cervical cancer cell-diagnostic cytological usefulness.
Poly(ADP-ribose) synthetase activity in human cervical cancer cell nuclei was found to be approximately twice that of normal cervical cell nuclei. Using antipoly(ADP-ribose) antibody, frozen section of normal tissue were stained by indirect-immunofluorescence and it was demonstrated that the fluorescence of nuclei in squamous cells decreased during maturation. Correspondingly, superficial cells appearing in smear specimens had less nuclear fluorescence compared with parabasal cells in the same smear. However, cancer cells in cervical smears had marked nuclear fluorescence using immunostaining and were easily distinguished from normal cells.
D002467:Cell Nucleus / Q000201:enzymology; D004796:Clinical Enzyme Tests; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D009244:NAD+ Nucleosidase / Q000032:analysis*; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology; D014626:Vaginal Smears
D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase