Influence of environmental factors upon the survival of Aujeszky's disease virus.
The survival of Aujeszky's disease (pseudorabies) virus outside the living host was found to be dependent on pH level and temperature. Virus inactivation occurred at a rate varying from 0.04 log10 per day at 4 degrees C to 0.6 log10 per day at 37 degrees C at the optimum pH levels of 6 to 8. Fluctuation of the temperature between 4 degrees C and 37 degrees C had no apparent effect upon the titre of the virus. At a steady temperature of -13 degrees C, the virus was rapidly inactivated at all tested pH levels. For long-term storage it is recommended that the pH should be adjusted to between 6 and 7, that suspensions should be kept at -90 degrees C and that freezing and thawing should be rapid so as to pass through the just subzero temperature zone as quickly as possible. Drying, both on glass and on gelatin, caused inactivation of the virus at all pH levels, as did exposure to ultraviolet light.
D003470:Culture Media; D011558:Herpesvirus 1, Suid / Q000254:growth & development* / Q000528:radiation effects; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014466:Ultraviolet Rays
D003470:Culture Media