Mitogenic activity in human embryonic fibroblasts early after infection by human cytomegalovirus.
We have characterized the nonspecific lymphocyte stimulation by extracts of human cytomegalovirus-infected human embryonic fibroblasts. Cell extracts prepared at 5 h postinfection (early extract) and 72 h postinfection (late extract) were both highly mitogenic in lymphocyte preparations from adult blood, cord blood, and rabbit blood. Maximum stimulation of the lymphocytes was observed on day 3 after the addition of early or late extract under optimal conditions. Early extract stimulated both the E-rosetting and the EAC-rosetting subpopulations of human lymphocytes. The mitogenic activity appeared before 5 h postinfection and was fairly stable at 30 degrees C for 5 h.
D000818:Animals; D000956:Antigens, Viral; D002457:Cell Extracts / Q000032:analysis* / Q000494:pharmacology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development* / Q000276:immunology; D004622:Embryo, Mammalian; D005312:Fetal Blood; D005347:Fibroblasts; D006801:Humans; D008213:Lymphocyte Activation; D008934:Mitogens / Q000032:analysis*; D011817:Rabbits; D013997:Time Factors; D014020:Tissue Extracts / Q000032:analysis*
D000956:Antigens, Viral; D002457:Cell Extracts; D008934:Mitogens; D014020:Tissue Extracts