[Cartilage and vitamin D in vitro (author's transl)].
Interactions between cartilage and vitamin D metabolism were studied in vitro. Chondrocytes were isolated from rabbit growth plate cartilage and cultured. These cells are able to transform 25-hydroxycholecalciferol in 24, 25-dihydroxycholecalciferol. Metabolic transformation is modulated by 1, 25-dihydroxycholecalciferol but not by parathyroid hormone neither by extracellular calcium concentrations. 24, 25-(OH) 2D3 and 1, 25-(OH) 2D3 are active on the cellular metabolism of cultured chondrocytes in a different way : 24, 25-(OH) 2D3 stimulates the proteoglycan synthesis in "mature" chondrocytes 1, 25-(OH) 2D3 increases DNA polymerase activities in chondrocytes during the logarithmic phase of division. Finally, specific sites of nuclear receptors of 24, 25-(OH) 2D3 are present in chondrocytes.
D000818:Animals; D002112:Calcifediol; D002117:Calcitriol / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism*; D002478:Cells, Cultured; D004100:Dihydroxycholecalciferols / Q000378:metabolism; D006887:Hydroxycholecalciferols / Q000378:metabolism; D011817:Rabbits; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism; D014807:Vitamin D / Q000378:metabolism*
C023892:25-hydroxycholecalciferol receptor; D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D014807:Vitamin D; D002117:Calcitriol; D002112:Calcifediol
Cartilage et vitamine D in vitro.