P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12.
The trg locus, which had been located at min 31 in the cotransduction gap in the terminus region of the chromosome of Escherichia coli, has been mapped by transduction with bacteriophage P1. This locus exhibited no cotransduction with fnr when rac+ strains were used. If rac strains were used, which removed approximately 27 kilobase pairs of DNA, trg and fnr exhibited 8.2% cotransduction. Although this mapping of trg at min 31.1 considerably reduces the size of the cotransduction gap, trg exhibited no cotransduction with a Tn10 insertion located on the other side of the gap at min 34.2.
D002633:Chemotaxis; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005690:Galactose / Q000494:pharmacology; D005798:Genes, Bacterial; D011995:Recombination, Genetic; D012266:Ribose / Q000494:pharmacology; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D012266:Ribose; D005690:Galactose