A solid-phase radioimmunoassay for quantitative measurement of class-specific antibodies against tick-borne encephalitis virus.
Antigen-coated polystyrene spheres are used for a solid-phase RIA for IgM and IgG antibodies against tick-borne encephalitis virus (TBE). The use of highly purified anti-mu and anti-gamma antibodies permitted the construction of standard curves from which quantitative values for TBE-specific IgG and IgM could be obtained. An antibody-blocking test identifies non-specific results.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D005189:False Positive Reactions; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008863:Microspheres; D011137:Polystyrenes; D011863:Radioimmunoassay / Q000379:methods
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011137:Polystyrenes