Immunofluorescent staining of nuclear antigen in lymphoid cells transformed by Herpesvirus papio (HVP).
An improved fixation method for antigen detection in lymphoblastoid cells is described. Herpesvirus papio nuclear antigen (HUPNA) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (ACIF). Antibody to HUPNA was detected in many human sera containing antibodies to Epstein-Barr virus capsid and nuclear antigen (EBNA). Rheumatoid arthritis sera showed a high incidence of both anti-EBNA and anti-HUPNA antibodies.
D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral / Q000096:biosynthesis; D000941:Antigens; D002467:Cell Nucleus / Q000276:immunology*; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation
D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral; D000941:Antigens; D005404:Fixatives