S-Adenosyl-L-methionine is a carbon donor in the conversion of benzo[alpha]pyrene to 6-hydroxymethylbenzo[alpha]pyrene by rat liver S-9.
In earlier studies we reported that 6-hydroxymethylbenzo[alpha]pyrene is a metabolite of benzo[alpha]pyrene and of 6-methylbenzo[alpha]pyrene when these substrates are incubated with homogenates or microsomal preparations of liver and microsomal preparations of subcutaneous tissue from Sprague--Dawley rats. However, the origin of the carbon donor was not established. We now report that S-adenosyl-L-methionine (SAM) can serve as a carbon donor in the metabolic formation of 6-hydroxymethylbenzo[alpha]pyrene from benzo[alpha]pyrene by the S-9 fraction of rat liver.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D001711:Biotransformation; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000378:metabolism*
D001580:Benzopyrenes; C017409:6-(hydroxymethyl)benzo(a)pyrene; D001564:Benzo(a)pyrene; D012436:S-Adenosylmethionine