A simple method for the isolation of basolateral plasma membrane vesicles from rat kidney cortex. Enzyme activities and some properties of glucose transport.
A procedure for preparing basolateral membrane vesicles from rat renal cortex was developed by differential centrifugation and Percoll density gradient centrifugation, and the uptake of D-[3H] glucose into these vesicles was studied by a rapid filtration technique. (Na+ + K+)-ATPase, the marker enzyme for basolateral membranes, was enriched 22-fold compared with that found in the homogenate. The rate of D-glucose uptake was almost unaffected by Na+ gradient (no overshoot).
D000469:Alkaline Phosphatase / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000201:enzymology*; D002499:Centrifugation, Density Gradient / Q000379:methods; D005947:Glucose / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000469:Alkaline Phosphatase; D000626:Aminopeptidases; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose