Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture.
After inoculation with JHM strain into DBT cell monolayers, a persistently infected DBT cell culture was established without producing typical cytopathic changes after about 15th passages. By immunofluorescence virus specific antigen was demonstrated in 10 to 15% DBT cells. This persistently infected culture (JHM-CC) was resistant to superinfection with parental JHM, but such resistance was not shown against vesicular stomatitis virus. JHM-CC virus produced small plaques on DBT cell monolayers. Temperature sensitive (TS) mutant, defective interfering (DI) particle or interferon was not detected in the JHM-CC. To intracerebral inoculation with JHM-CC virus, cortisone treated ICR mice survived without showing clinical signs, however, demyelinating lesions were produced in the brain and spinal cord of them.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003333:Coronaviridae Infections; D003588:Cytopathogenic Effect, Viral; D003711:Demyelinating Diseases / Q000209:etiology; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D006517:Murine hepatitis virus / Q000254:growth & development* / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014762:Viral Interference
D000956:Antigens, Viral