Absence of IgG antibodies to TDI-HSA in a radioimmunological study.
Sera of ninety-one workers with respiratory symptoms related to occupational exposure to toluene diisocyanate (TDI) were tested by a solid phase radioimmunoassay using polyvinyl plates, in an attempt to demonstrate specific IgG antibodies to a conjugate of TDI with human serum albumin (TDI-HSA). Different conjugates were prepared. In our radioimmunoassay a TDI-HSA 1600 (1600 micrograms TDI/mg HSA), obtained at alkaline pH with borate buffer, was used; this conjugate was immunologically reactive and did not contain protein macro-aggregates. No difference in percent of binding of radiolabelled anti-human IgG to TDI-HSA coated on plastic plates was found between exposed workers (asthmatic and non-asthmatic) and fifteen normal subjects. This may suggest that either TDI-induced bronchial asthma is not mediated by IgG antibodies, or TDI-HSA conjugate is unsuitable to be detected by anti-isocyanate IgG.
D000818:Animals; D001249:Asthma / Q000276:immunology; D003485:Cyanates / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis*; D009784:Occupational Diseases / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D012130:Respiratory Hypersensitivity / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology*; D014051:Toluene 2,4-Diisocyanate / Q000276:immunology*
D003485:Cyanates; D007074:Immunoglobulin G; D012709:Serum Albumin; D014051:Toluene 2,4-Diisocyanate