Enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus-specific IgM antibodies.
Herpes simplex virus (HSV)-specific IgM in human serum could be detected by a microplate enzyme-linked immunosorbent assay, using extracts of HSV-infected cells as antigen. Peroxidase-conjugated anti-human IgM was used to detect human IgM bound to viral antigen. Pretreatment of sera with protein A-bearing staphylococcus or with aggregated human IgG was necessary to eliminate false-positive results caused by the presence of rheumatoid factor. Specificity controls included sera of patients with other herpes group virus infections.
D000914:Antibodies, Viral / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006561:Herpes Simplex / Q000276:immunology*; D006564:Herpesviridae / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D012217:Rheumatoid Factor; D018139:Simplexvirus / Q000276:immunology*; D013205:Staphylococcal Protein A
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A; D012217:Rheumatoid Factor