Effect of chemical modification of a histidine and a lysine residue of pea seed nucleoside diphosphate kinase.
Chemical modification of a histidine and lysine residue inactivates pea seed nucleoside diphosphate kinase (NDP kinase). Thus there seems to be a reactive lysine residue, at the active site of pea seed NDP kinase, in addition to the histidine residue phosphorylated by the substrate ATP as a consequence of the enzyme reaction. The presence of a reactive lysine at the active site of the enzyme could explain why a small amount of N-epsilon-phospholysine, as well as 1-phosphohistidine and 3-phosphohistidine, is formed on alkaline hydrolysis of the enzyme.
D007887:Fabaceae / Q000201:enzymology*; D006639:Histidine; D008239:Lysine; D009701:Nucleoside-Diphosphate Kinase; D010770:Phosphotransferases; D010946:Plants, Medicinal
D006639:Histidine; D010770:Phosphotransferases; D009701:Nucleoside-Diphosphate Kinase; D008239:Lysine