[5-Methylcytosine content in erythroleukemic cells of rats after induction with dimethylsulfoxide].
Erythroleucemic cells of rats were induced by DMSO for the terminal erythroid differentiation. The base composition and the 5 MC content of the DNA were analyzed by HPLC and a significant difference between non-induced and induced cells was not found. The analysis of labelled DNA by the restriction enzymes Hha I, Hpa II, and Msp I did not result in a different pattern before and after the induction. These results suggest that the 5 MC content of the DNA does not change during the induced terminal erythroid differentiation.
D044503:5-Methylcytosine; D000818:Animals; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004121:Dimethyl Sulfoxide; D007942:Leukemia, Experimental / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats
D004273:DNA, Neoplasm; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes; D004121:Dimethyl Sulfoxide
5-Methylcytosin-Gehalt in Rattenerythroleukämie-Zellen nach Induktion mit Dimethylsulfoxid.