Synthesis and physicochemical properties of two analogs of poly(dA): poly(2-aminopurine-9-beta-D-deoxyribonucleotide) and poly 2-amino-deoxyadenylic acid.
Polymerization of chemically synthesized dn2h6ATP and dn2ATP by deoxynucleotidyl transferase from calf thymus furnished poly(dn2h6A) and poly(dn2A) respectively. The synthetic polynucleotides were characterized by spectroscopic, ultracentrifugation and enzymatic methods. In polynucleotide-polynucleotide interaction, poly(dn2h6A) and poly(dn2A) behaved like analogs of poly(dnA).
D000818:Animals; D002417:Cattle; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000138:chemical synthesis; D007202:Indicators and Reagents; D008722:Methods; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011061:Poly A / Q000031:analogs & derivatives*; D011071:Poly T; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis*; D011131:Polyribonucleotides; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013950:Thymus Gland / Q000201:enzymology
D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D011061:Poly A; D011071:Poly T; D004253:DNA Nucleotidylexotransferase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I