Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element.
The cellular oncogene c-mos is rearranged in a mouse myeloma and the tumour mRNA contains transcripts hybridizing with a v-mos probe. The rearranged gene (rc-mos) was cloned in lambda phage and shown to transform mouse fibroblasts in transfection assays, rc-mos differs from its progenitor, c-mos, only at the 5' end of the gene, where c-mos sequences have been substituted by a novel cellular DNA fragment. This fragment contains a 159-base pair (bp) insertion sequence (IS)-like element localized immediately 5' to the junction with c-mos. This is the first demonstration in a non-virally-induced tumour of activation of a cellular oncogene by a mechanism possibly involving DNA transposition.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D004273:DNA, Neoplasm / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D051379:Mice; D009194:Myeloma Proteins / Q000235:genetics*; D009374:Neoplasms, Experimental / Q000235:genetics; D009857:Oncogenes; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D004273:DNA, Neoplasm; D009194:Myeloma Proteins