title: Leukotriene A4: preparation and enzymatic conversion in a cell-free system to leukotriene B4.
abstract: Treatment of leukotriene A4 (LTA4) methyl ester with sodium hydroxide in aqueous methanol at 4 degrees C afforded LTA4, the presence of which was inferred from the UV spectrum of the compound, its rate of reaction with water, and the identity of the hydration products obtained. The half-life of LTA4 in water (pH 7.4, room temperature) was increased from 14 to 500 s by 1 mg/ml of bovine serum albumin. This stabilized (chiral) LTA4 was converted to LTB4 by an epoxide hydrolase activity in the 100,000 x g supernatant fraction from sonified rat basophilic leukemia cells. Neither the ester of LTA4 nor the biologically incorrect enantiomer of LTA4 was metabolized to LTB4 under these conditions.
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis / Q000378:metabolism*; D001491:Basophils / Q000201:enzymology; D002633:Chemotaxis; D004355:Drug Stability; D004851:Epoxide Hydrolases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007942:Leukemia, Experimental / Q000201:enzymology*; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000096:biosynthesis*; D009504:Neutrophils / Q000502:physiology; D051381:Rats; D012710:Serum Albumin, Bovine
chemical_list: D001095:Arachidonic Acids; D017572:Leukotriene A4; D007975:Leukotriene B4; D012710:Serum Albumin, Bovine; D004851:Epoxide Hydrolases