title: Multiple components in restriction enzyme digests of mammalian (insectivore), avian and reptilian genomic DNA hybridize with murine immunoglobulin VH probes.
abstract: High molecular weight genomic DNAs isolated from an insectivore, Tupaia, and a representative reptilian, Caiman, and avian, Gallus, were digested with restriction endonucleases transferred to nitrocellulose and hybridized with nick-translated probes of murine VH genes. The derivations of the probes designated S107V (1) and mu 107V (2,3) have been described previously. Under conditions of reduced stringency, multiple hybridizing components were observed with Tupaia and Caiman; only mu mu 107V exhibited significant hybridization with the separated fragments of Gallus DNA. The nick-translated S107V probe was digested with Fnu4H1 and subinserts corresponding to the 5' and 3' regions both detected multiple hybridizing components in Tupaia and Caiman DNA. A 5' probe lacking the leader sequence identified the same components as the intact 5' probe, suggesting that VH coding regions distant as the reptilians may possess multiple genetic components which exhibit significant homology with murine immunoglobulin in VH regions.
mesh_terms: D000489:Alligators and Crocodiles / Q000276:immunology*; D000818:Animals; D001666:Binding Sites, Antibody / Q000235:genetics*; D002645:Chickens / Q000276:immunology*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008099:Liver / Q000276:immunology; D051379:Mice; D009693:Nucleic Acid Hybridization; D012104:Reptiles / Q000276:immunology*; D013045:Species Specificity; D014417:Tupaia / Q000276:immunology*; D014418:Tupaiidae / Q000276:immunology*
chemical_list: D007135:Immunoglobulin Variable Region; D004247:DNA; D004262:DNA Restriction Enzymes