title: Respiration of the rumen ciliate Dasytricha ruminantium Schuberg.
abstract: The endogenous respiration of the rumen ciliate Dasytricha ruminantium maintained under an O2 tension of 2kPa (approximately 0.02 atm) was partially inhibited by KCN (40% inhibition) and NaN3 (58% inhibition). The organisms lack cytochromes, and sensitivity of respiration to KCN, NaN3, chloroquine and quercetin suggest that the operation of flavoprotein-iron-sulphur-mediated electron transport. As in Tritrichomonas foetus, hydrogenosomal respiration can be stimulated by the addition of CoA in the presence of 0.025% Triton X-100; stimulation by ADP was not detected. Stimulation of pyruvate-supported O2 uptake by Pi suggests that acetate is produced via acetyl phosphate.
mesh_terms: D000084:Acetate Kinase / Q000378:metabolism; D002457:Cell Extracts; D016798:Ciliophora / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003065:Coenzyme A / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D010709:Phosphate Acetyltransferase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor)
chemical_list: D002457:Cell Extracts; D010709:Phosphate Acetyltransferase; D010770:Phosphotransferases; D017851:Phosphotransferases (Carboxyl Group Acceptor); D000084:Acetate Kinase; C019285:butyrate kinase; D003065:Coenzyme A