The CAMP test performed by using Staphylococcus aureus sphingomyelinase (beta-haemolysin) and Clostridium perfringens lecithinase.
The CAMP test was performed by employing Staphylococcus aureus beta-haemolysin (sphingomyelinase) and Clostridium perfringens alpha-toxin (lecithinase) for identification of group B streptococci on blood agar, using blood from different origin. Partial purification of Cl perfringens alpha-toxin was carried out by means of sheep erythrocytes. With the toxin preparations described positive CAMP reaction was obtained only on sheep blood agar. False positives with regard to group A streptococci could not be avoided by either of the methods.
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification; D001431:Bacteriological Techniques; D002417:Cattle; D003016:Clostridium perfringens / Q000201:enzymology; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D010740:Phospholipases; D012756:Sheep / Q000097:blood; D013108:Sphingomyelin Phosphodiesterase; D013211:Staphylococcus aureus / Q000201:enzymology; D013292:Streptococcus agalactiae / Q000302:isolation & purification*
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D010740:Phospholipases; D013108:Sphingomyelin Phosphodiesterase