title: Apurinic endonuclease from Drosophila melanogaster: reduced enzymatic activity in excision-deficient mutants of the mei-9 and mus(2)201 Loci.
abstract: An endonuclease activity, which is specific for partially depurinated PM2 DNA, has been assayed in extracts prepared from cultured cells and larval brain ganglia of Drosophila melanogaster. Activity detected in repair-proficient cells is stimulated by Mg++ and is inhibited by EDTA. Extracts prepared from established cell cultures of the excision-deficient strain mei-9a and from larval brain ganglia derived from y mei-9a, mei-9L1, sn3 mei-9D2 and sn3 mei-9D4 are partially deficient in this activity. At least one allele of a second excision-deficient strain, cn mus (2)201D1, also shows reduced AP-endonuclease activity in ganglia extracts.
mesh_terms: D000818:Animals; D001080:Apurinic Acid / Q000235:genetics; D002478:Cells, Cultured; D046508:Culture Techniques; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D003851:Deoxyribonucleases / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005724:Ganglia; D009154:Mutation
chemical_list: D001080:Apurinic Acid; D003851:Deoxyribonucleases; D004720:Endonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase