Fragments of the simian virus 40 transforming gene facilitate transformation of rat embryo cells.
Segments of the simian virus 40 (SV40) genome that encode only fragments of large tumor antigen can facilitate immortalization of secondary rat embryo cells. The phenotypes of the immortalized cells range from nearly "normal" to fully transformed. All of the cell lines contain SV40 sequences and express unstable NH2-terminal fragments of large tumor antigen. SV40 small tumor antigen does not appear to be essential for either immortalization or transformation.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004622:Embryo, Mammalian; D005814:Genes, Viral; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes