title: Integration specificity of an artificial kanamycin transposon constructed by the in vitro insertion of an internal Tn5 fragment into IS2.
abstract: IS2 has been marked genetically by the in vitro insertion into its HindIII site of a 3.3 Kb HindIII fragment of Tn5 conferring resistance to kanamycin. The transposition of the IS2::Km, thus obtained, to lambda has been found and insertion sites were characterised. Each of ten independent IS2::Km insertions were found at the same site at 61.2% of the lambda map, always in the same orientation (orientation II relative to the xis gene). The integration sites of IS2::Km in five of the kanamycin-transducing phages were determined by DNA sequence analysis, and were found to be identical at the nucleotide level. Further transposition of IS2::Km from lambda to the bacterial chromosome was demonstrated.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005819:Genetic Markers; D007612:Kanamycin / Q000494:pharmacology*; D011995:Recombination, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004279:DNA, Viral; D005819:Genetic Markers; D007612:Kanamycin