Direct demonstration of genetic alterations at the dihydrofolate reductase locus after gamma irradiation.
Gamma ray-induced mutants of Chinese hamster ovary cells lacking dihydrofolate reductase activity were screened for DNA sequence changes at the locus specifying this activity by using a cloned cDNA probe. Two of nine mutants screened displayed an altered restriction fragment pattern suggesting the occurrence of DNA deletions or rearrangements.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes; D005720:Gamma Rays; D009693:Nucleic Acid Hybridization; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes