Loss of high frequency of sister chromatid exchanges in Epstein-Barr virus-established lymphoblastoid cell lines from two patients with Bloom's syndrome.
Seven lymphoblastoid cell lines were established through transformation by Epstein-Barr virus of peripheral blood lymphocytes from two patients with Bloom's syndrome (BS), the parents of a patient, and normal controls. High baseline levels of sister chromatid exchanges (SCEs) in peripheral blood lymphocytes of BS were reduced to about 10% of their initial value in BS lymphoblastoid cell lines, and the elevation of SCE frequencies induced by ethylmethanesulfonate was the same as in controls.
D000293:Adolescent; D001816:Bloom Syndrome / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002648:Child; D003434:Crossing Over, Genetic; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D012854:Sister Chromatid Exchange