Effect of in vitro preincubation of polymorphonuclear leukocytes on formylmethionyl-leucyl-phenylalanine-induced chemiluminescence.
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence. The chemotactic peptide formylmethionyl-leucyl-phenylalanine induced a two-peak chemiluminescence response in PMNL. The response was modified, both in magnitude and in the time course of the response, when PMNL were incubated at 22 degrees C for 15 to 120 min before the addition of stimulus. The cellular response to formylmethionyl-leucyl-phenylalanine was also changed by the addition of cell-free supernatants from stored PMNL suspensions to freshly prepared PMNL before the addition of formylmethionyl-leucyl-phenylalanine. This indicated that PMNL during storage released substances that changed the chemiluminescence response. A similar change could be obtained by addition of purified myeloperoxidase to freshly prepared PMNL before the addition of stimulus, indicating that the myeloperoxidase-hydrogen peroxide system can function as a modulator of the cellular response to chemotactic factors.
D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D008715:Methionine / Q000031:analogs & derivatives*; D009239:N-Formylmethionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D001386:Azides; D009842:Oligopeptides; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase