Modification of the poliovirus capsid by ultraviolet light.
Ultraviolet (UV) irradiation of type I poliovirus resulted in a modified (M) particle that had lost infectivity, lacked ability to adsorb to HeLa cells, lacked VP4, and reduced in S value. Additional irradiation resulted in the loss of VP2, further reduction in S value, and permeability of the capsid to RNAse, This particle (C) as well as M contain the genome. Acid pH (5.5-65) and sulfhydryl-reducing substances (dithiothreitol. reduced glutathione, and L-cysteine) inhibited UV-induced modification of the capsid. UV irradiation at alkaline pH (7.5-8.5) resulted in more extensive modification of the capsid than irradiation at neutral pH. Ionic compounds were found to inhibit the modifying reaction.
D000327:Adsorption; D002122:Calcium Chloride / Q000494:pharmacology; D002213:Capsid / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006863:Hydrogen-Ion Concentration; D017955:Poliovirus / Q000502:physiology / Q000528:radiation effects*; D012965:Sodium Chloride / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000528:radiation effects*
D013439:Sulfhydryl Reagents; D014764:Viral Proteins; D012965:Sodium Chloride; D002122:Calcium Chloride