title: Semi-met oxidation level of chalcogenide derivatives of methemerythrin. Mössbauer and EPR studies.
abstract: Conclusive evidence is presented for an S = 1/2 spincoupled pair of high spin ferric and ferrous ions in the major reaction product of sulfide with the met form of the non-heme iron oxygen-carrying protein hemerythrin. Evidence for an analogous selenide derivative is also reported. Mössbauer and EPR spectroscopy establish (a) the charge and spin states of the individual iron atoms in sulfidehemerythrin as Fe(III), S = 5/2, and Fe(II), S = 2, and (b) the existence of an antiferromagnetic exchange interaction that couples the two spins to a resultant spin S = 1/2. The combined Mössbauer and EPR data confirm the correctness of the formulation first proposed for semi-methemerythrin by Harrington, P.C., de Waal, D.J.A., and Wilkins, R.G. ((1978) Arch. Biochem. Biophys. 191, 444-451) and furthermore show that a majority of the iron centers in the protein can be stabilized at this oxidation level. The results also demonstrate a new route to semi-methemerythrin. A titration of methemerythrin with selenide indicates that this derivative forms by a two step process consisting of first, reduction to the semi-met oxidation level by selenide and second, binding of selenide to either one or both irons.
mesh_terms: D000818:Animals; D002599:Chalcone / Q000031:analogs & derivatives / Q000378:metabolism*; D003460:Crystallization; D004578:Electron Spin Resonance Spectroscopy; D006422:Hemerythrin / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D011427:Propiophenones / Q000378:metabolism*
chemical_list: D006422:Hemerythrin; D008667:Metalloproteins; D011427:Propiophenones; C019004:methemerythrin; D002599:Chalcone