The subcellular fractionation of the bovine caudate nucleus.
Two synaptosomal fractions could be obtained from bovine caudate nucleus on sucrose density gradients one of which had a much greater capacity for 'high affinity' choline uptake than the other but comparable amounts of CAT and choline kinase activity. Specific binding of QNB was widely distributed among all the subcellular fractions except the mitochondrial fraction and in quantitative terms by far the greatest amount was in the microsomal fraction. Only the microsomal fraction contained measurable amounts of glycerophosphocholine phosphodiesterase.
D000818:Animals; D002417:Cattle; D002421:Caudate Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D002794:Choline / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D008928:Mitochondria / Q000648:ultrastructure; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism / Q000648:ultrastructure*
D011813:Quinuclidinyl Benzilate; D002795:Choline O-Acetyltransferase; D002797:Choline Kinase; D010727:Phosphoric Diester Hydrolases; C020677:glycerophosphocholine phosphodiesterase; D002794:Choline