Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk.
We have developed a sandwich enzyme immunoassay in order to measure quantitatively mouse mammary tumor virus (MMTV) in mouse milk. In this assay, the antibody-beta-D-galactosidase complex and antibody-bound silicon rubber pieces pieces as solid phase are used. The assay is able to detect 10 ng/ml of MMTV in the milk sample.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005260:Female; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008892:Milk / Q000382:microbiology*; D012826:Silicone Elastomers; D001616:beta-Galactosidase
D000956:Antigens, Viral; D007106:Immune Sera; D012826:Silicone Elastomers; D001616:beta-Galactosidase