Cloning and identification of the product of the dnaE gene of Escherichia coli.
We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli chromosome was determined. A unique HindIII restriction endonuclease site within the cloned segment of DNA was identified as a site required for expression of the dnaE gene. By using the maxicell plasmid-directed protein synthesizing system, we demonstrated that dnaE codes for the alpha subunit of DNA polymerase III.
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004258:DNA Polymerase III / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids
D001426:Bacterial Proteins; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII