The effect of inorganic pyrophosphate (PPi) anions on the in vitro collagen fibril formation.
This paper presents a study on the effect of sodium salt of pyrophosphate (Na-PPi) in different concentrations (1-10 mMol/l) on the in vitro collagen fibril formation. Collagen was mixture of type I and III collagen dissolved in neutral buffer containing sodium chloride of 1.0 mol/l. Na-PPi added to collagen delayed the fibrillary precipitation of collagen from the solution. Electronmicroscopically the fibrils are of native collagen type, but they are thicker and show a pattern of fine substriation. Calcium and other divalent cations showed differences in their influence on the effect of Na-PPi. This experimental model was developed in order to interpret the supposed role of PPi in the inhibition of apatite crystal formations as well as the fibrogenic effect of its calcium salt in PPi-arthropathy. The effect of PPi on the collagen fibril formation should also be taken into consideration.
D000818:Animals; D000838:Anions; D002118:Calcium / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis*; D011756:Diphosphates / Q000494:pharmacology*; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D051381:Rats
D000838:Anions; D011756:Diphosphates; D003094:Collagen; D008274:Magnesium; D002118:Calcium