Ly- and ecotropic MuLV antigens are separate entities.
Murine alloantisera to lymphocyte (Ly) alloantigens also contain antibodies to murine leukaemia viruses (MuLV) in high titre. A number of such sera were extensively absorbed with ecotropic AKR murine leukaemia virus to remove contaminating anti-MuLV antibodies. The virus-absorbed and unabsorbed antisera were then examined by the radioimmune precipitation (RIP) assay for anti-MuLV antibodies, and for anti-Ly antibodies by cytotoxicity assays against specific target cells. The results presented here demonstrate that whilst virus absorption markedly reduced the anti-viral titres of the alloantisera they had no effect on the anti-Ly titres of any of them. This indicates that none of the Ly antigens which was examined cross reacts with the antigens of ecotropic AKR-MuLV and further suggests that Ly antigens are not encoded by the genes of endogenous ecotropic MuLVs. It was also demonstrated that the majority of the contaminating anti-MuLV antibodies present in anti-Ly-6.2 antiserum recognize viral envelope antigens rather than the internal structural proteins of the virus.
D000818:Animals; D000918:Antibody Specificity; D000950:Antigens, Ly / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D003429:Cross Reactions; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000276:immunology; D009052:Leukemia Virus, Murine / Q000276:immunology*; D051379:Mice
D000950:Antigens, Ly; D000956:Antigens, Viral; D007518:Isoantibodies