Properties of a DNA-binding protein in a culture medium of thymus cells.
A DNA-binding protein, which migrated as one major protein band, with a molecular weight of 14,000, on sodium dodecylsulfate polyacrylamide gel, was purified from a culture medium of mouse thymus cells. The interaction of the isolated protein with DNA in vitro was assayed by a nitrocellulose filter binding technique. Equilibrium competition experiments demonstrated that the DNA-binding protein had the ability to differentiate among sequences of polynucleotides, indicating that the DNA-binding protein-DNA interaction was at least partially specific. This protein increased the helix melting temperature of DNA and inhibited the incorporation of [3H]dTMP into DNA by the DNA polymerase of calf thymus in vitro.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004265:DNA Helicases / Q000302:isolation & purification* / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins; D007700:Kinetics; D008214:Lymphocytes / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D004257:DNA Polymerase II; D004265:DNA Helicases