title: Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos.
abstract: RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5-10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53 +/- 1 bp, 55 +/- 1 bp and 270 +/- 1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.
mesh_terms: D001482:Base Composition; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D005796:Genes; D005914:Globins / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics; D006801:Humans; D007945:Leukemia, Lymphoid; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012315:RNA Caps / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D006454:Hemoglobins; D012315:RNA Caps; D012333:RNA, Messenger; D005914:Globins; D004262:DNA Restriction Enzymes