Protection against hydroxyurea-induced cytotoxic effects in L5178Y cells by free radical scavengers.
Exposure of L5178Y cells in culture to 1 mM hydroxyurea (HU) for 3 h followed by 24 h incubation in an HU-free medium induced an abnormal enlargement of about 40% of the cells in the population and post-treatment reduction of DNA synthesis in comparison with control cells. These effects were used to examine the protection afforded by free radical scavengers against HU-induced cytotoxicity. It has been found that with careful choice of conditions (suitable concentration of the protective agent, pretreatment of cells) substantial protective effect of alpha-tocopherol, sodium benzoate, acetylsalicylic acid, catalase, peroxidase or superoxide dismutase can be achieved.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002374:Catalase / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D007527:Isoenzymes / Q000494:pharmacology*; D007700:Kinetics; D007940:Leukemia L5178 / Q000503:physiopathology*; D007942:Leukemia, Experimental / Q000503:physiopathology*; D051379:Mice; D009195:Peroxidase; D010544:Peroxidases / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
D001565:Benzoates; D007527:Isoenzymes; D014810:Vitamin E; D019817:Benzoic Acid; D010544:Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D001241:Aspirin; D006918:Hydroxyurea