Quantitation of estrogen effect on Xenopus laevis albumin mRNA levels by hybridization to cloned albumin cDNA.
The isolation and characterization of a Xenopus laevis albumin cDNA clone, pUN18, is described. This clone was identified as a X. laevis albumin cDNA clone by hybridization-mRNA selection-translation and by other techniques. The clone was used in hybridizations to determine the effect of estrogen on albumin synthesis in livers of male X. laevis. We find that albumin mRNA levels remain relatively constant during the massive induction of vitellogenin mRNA synthesis and vitellogenin secretion which follow administration of estrogen to male X. laevis.
D000418:Albumins / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004958:Estradiol / Q000494:pharmacology*; D008074:Lipoproteins / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*; D014819:Vitellogenins / Q000235:genetics*; D014981:Xenopus
D000418:Albumins; D008074:Lipoproteins; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol; D004247:DNA; D004262:DNA Restriction Enzymes