Establishment and propagation of a bovine leukaemia virus-producing cell line derived from the leukocyte of a leukaemic cow.
A cell line (LB59Ly) derived from the leukocytes of a leukaemic cow was established as a monolayer, which spontaneously released large amounts of a retrovirus. This virus was found to be indistinguishable from the bovine leukaemia virus (BLV) produced by the commonly used high-producing heterologous cell line (FLK-BLV). Like the latter, its reverse transcriptase activity was greater in the presence of Mg2+ cations than in the presence of Mn2+ cations; its polyacrylamide gel electrophoresis pattern showed the presence of gp51, p24, p15, p12 and p10, and the antigenicity of the two major proteins completely cross-reacted with those of BLV from FLK-BLV cells. The virus was infectious and induced early and late polykaryocytosis, the specificity of which was demonstrated by use of specific anti-BLV sera.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D002460:Cell Line; D007938:Leukemia / Q000097:blood / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000254:growth & development* / Q000276:immunology / Q000502:physiology; D007962:Leukocytes / Q000166:cytology; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000662:veterinary*; D012190:Retroviridae / Q000254:growth & development*