title: Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions.
abstract: Our previous studies indicated that fusion products with one functional nucleus but organelles of the two fusion partners (i.e. heteroplastomic cybrids) could be obtained by fusing X-irradiated (cytoplasmic donor) with non-irradiated (recipient) Nicotiana protoplasts. The present report deals with the analysis of mitochondria in cybrid populations resulting from the fusion of donor Nicotiana tabacum protoplasts with recipient protoplasts having a N. Sylvestris nucleus but chloroplasts of an alien Nicotiana species, and exhibiting cytoplasmic male sterility. The two fusion parents showed significant differences in restriction patterns of their chloroplast and mitochondrial DNA. Four groups of cybrid plants were obtained by this fusion. All had N. sylvestris nuclei but contained either donor or recipient chloroplasts and had either sterile or fertile anthers. There was no correlation between anther fertility and chloroplast type. The mitochondrial DNA restriction patterns of sterile cybrids were similar to the respective patterns of the sterile fusion partner while the mitochondrial DNA restriction patterns of the fertile cybrids were similar to the respective patterns of the fertile fusion partner. The results indicate an independent assortment of chloroplasts and mitochondria from the heteroplastomic fusion products.
mesh_terms: D002459:Cell Fusion; D002736:Chloroplasts / Q000502:physiology; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D011523:Protoplasts / Q000502:physiology; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes