Inhibitor effect of polyamines on reduction of cytochrome C by superoxide anion.
The interaction of polyamines with enzymatically generated free radicals was investigated. The superoxide anion (O-2) was generated in vitro using the xanthine oxidase-hypoxanthine system. Our results show that spermidine or spermine at different concentrations (20-200 mM) inhibit the reduction of cytochrome c; the highest levels of inhibition were obtained adding 200 mM spermidine or spermine. Putrescine (200 mM) affected the reduction of cytochrome c very little.
D003574:Cytochrome c Group / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology*; D011073:Polyamines / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D013053:Spectrophotometry; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013481:Superoxides / Q000494:pharmacology*
D003574:Cytochrome c Group; D011073:Polyamines; D013481:Superoxides; D013096:Spermine; D010100:Oxygen; D013095:Spermidine; D011700:Putrescine