Equivalency of endothelial cell growth supplement to irradiated feeder cells in carcinogen-induced morphologic transformation of Syrian hamster embryo cells.
Endothelial cell growth supplement (ECGS), an extract of bovine neural tissue with growth-promoting properties for human endothelial and epithelial cells and for mouse BALB/c fibroblast-like cells, can be substituted for feeder cells in a quantitative 7-day Syrian hamster embryo cell colony in vitro model of carcinogenesis. Inclusion of 50 or 100 micrograms ECGS/ml medium throughout the 7-day growth period produced results equal to those obtained with feeder cells. The frequency and morphology of normal fibroblast colonies and carcinogen-induced morphologically transformed cell colony growth in the presence of ECGS were similar to those in the presence of feeder cells. A positive dose-response relationship in transformation by benzo[a]pyrene occurred. The frequency of transformed colonies following UV irradiation and treatment of the cells with the cocarcinogenic tumor promoter 12-O-tetradecanoylphorbol 13-acetate was greatly augmented, and lymphotoxin, a lymphokine with anticarcinogenic activity, reduced transformation. Thus ECGS can substitute for feeder cells in supporting in vitro transformation and eliminates a potential complex source of variability for studies where interaction(s) with feeder cells are a consideration. The mechanics of this model system was simplified, and its versatility for the study of physiologic, carcinogenic, and other pathophysiologic processes was broadened.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000494:pharmacology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000528:radiation effects; D003043:Cocarcinogenesis; D006224:Cricetinae; D003584:Cytological Techniques; D004622:Embryo, Mammalian; D006133:Growth Substances / Q000494:pharmacology*; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D008647:Mesocricetus; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays
D001580:Benzopyrenes; D006133:Growth Substances; D008233:Lymphotoxin-alpha; D001564:Benzo(a)pyrene; D013755:Tetradecanoylphorbol Acetate