Effect of transmethylation-reaction and increased levels of cAMP on superoxide generation of guinea-pig macrophages induced with wheat germ agglutinin and phorbor myristate.
Superoxide (O2-) generation of guinea-pig macrophages induced by wheat germ agglutinin (WGA) was suppressed to a great extent by the inhibition of transmethylation with 3'-deazaadenosine. When macrophages were stimulated with phorbor myristate (PMA) instead of WGA, the suppression of O2- generation of macrophages was observed to be slight despite the presence of 3'-deazaadenosine. These results were confirmed under various conditions. Thus the WGA-stimulated O2- generation of macrophages is probably associated with transmethylation, but the PMA-stimulated O2- generation is not. WGA-stimulated O2- generation of macrophages was also inhibited in the presence of dibutyryl cAMP or prostaglandin E2 (PGE2), substances that increase intracellular cAMP, but PMA-stimulated O2- generation was only slightly affected by these compounds. These results suggest that the mechanism for O2- generation of macrophages caused by WGA is different from that for O2- generation caused by PMA.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone; D006168:Guinea Pigs; D037102:Lectins / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008745:Methylation; D010100:Oxygen / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014372:Tubercidin / Q000494:pharmacology; D014909:Wheat Germ Agglutinins
D037102:Lectins; D010704:Phorbols; D011458:Prostaglandins E; D014909:Wheat Germ Agglutinins; C018258:3-deazaadenosine; D013481:Superoxides; D003994:Bucladesine; D000242:Cyclic AMP; D015232:Dinoprostone; D014372:Tubercidin; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen