A new restriction endonuclease from Citrobacter freundii.
CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacterfreundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5' -protruding tetranucleotide ends GGCC.
D001483:Base Sequence; D002954:Citrobacter / Q000201:enzymology*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity
D004262:DNA Restriction Enzymes