Antibody to the nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is specific for polyoma middle T antigen and inhibits in vitro kinase activity.
Middle T antigen of polyoma virus has an associated tyrosine kinase activity which phosphorylates tyrosine residue 315 on middle T in immunoprecipitates. A peptide representing the sequence of middle T from residue 311 to 319 has been synthesized. This peptide acts as a weak inhibitor of the kinase reaction. An antiserum has been raised against this peptide after conjugation to bovine serum albumin. The antibody is middle T-specific. Middle T antigen precipitated by this serum is largely inactive in the kinase reaction. Dissociation of the immune complex with peptide releases middle T in a kinase-active form.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D000952:Antigens, Polyomavirus Transforming; D000955:Antigens, T-Independent / Q000276:immunology*; D002478:Cells, Cultured; D002918:Chymotrypsin; D007668:Kidney; D051379:Mice; D009842:Oligopeptides / Q000276:immunology*; D010446:Peptide Fragments / Q000032:analysis; D010770:Phosphotransferases / Q000378:metabolism*; D011120:Polyomavirus / Q000201:enzymology / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000952:Antigens, Polyomavirus Transforming; D000955:Antigens, T-Independent; D009842:Oligopeptides; D010446:Peptide Fragments; C036204:glutamyl-glutamyl-glutamyl-glutamyl-tyrosyl-methionyl-prolyl-methionyl-glutamic acid; D010770:Phosphotransferases; D002918:Chymotrypsin