A comparison of resolution-enhancement methods in saturation-transfer EPR. 15N isotopically substituted spin labels and 35 GHz high-frequency operation.
The use of (15)N isotopic substitution and 35 GHz (Q-band) operation as resolution enhancement methods in saturation-transfer electron paramagnetic resonance (ST-EPR) are compared. We find that both methods offer roughly comparable enhancements in ST-EPR resolution. The most powerful approach to resolving complex ST-EPR spectral behavior, however, will probably be the combined use of multiple-frequency X- and Q-band operation with (15)N isotopically substituted spin labels.
D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes; D013113:Spin Labels; D013997:Time Factors
D009587:Nitrogen Isotopes; D013113:Spin Labels