title: Mitogen-induced changes in glycolytic enzymes of mouse lymphocytes: influence of insulin on cell activation in vitro.
abstract: Activated mouse spleen lymphocytes have increased amounts of the glycolytic enzymes pyruvate kinase and lactate dehydrogenase. No changes were found in hexokinase and in the gluconeogenic enzyme fructose 1,6-diphosphatase. Concanavalin A-activated T cells give higher activities of those enzymes than lipopolysaccharide-activated B lymphoblasts. Insulin treatment results in a stronger increment of the enzyme activity of mitogen-activated cells. Insulin inhibits the initial proliferation induced by either Con A or LPS, but a 50% increase in antibody-forming cells was found in LPS-treated cultures. Insulin may favour the differentiation of activated cells by increasing the rate of the glycolytic pathway.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: D007328:Insulin; D008934:Mitogens; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase; D006597:Fructose-Bisphosphatase