[Computer analysis of chromatin fragmentation with nucleases].
Computerizing of the densitograms of chromatin hydrolysate DNA is offered. It is based on searching for gaussoids depicting the peaks of individual oligomers so that the resultant curve corresponded to the experimental curve to a high enough accuracy. The method suggested was used to study the time course of mononucleosome and oligomer accumulation during chromatin fragmentation with endogenous nuclease. Comparison of the experimental data to a model calculated for break down of 500-nucleosome chromatin fragments demonstrates that in chromatin, the release of mono-, di- and trinucleosomes is shifted towards nucleosomes during break down induced by endogenous nuclease.
D000818:Animals; D002843:Chromatin / Q000032:analysis*; D002847:Chromatography, Agarose; D003201:Computers; D004247:DNA / Q000032:analysis*; D003720:Densitometry; D004720:Endonucleases; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D051381:Rats
D002843:Chromatin; D004247:DNA; D004720:Endonucleases
Komp'iuternyĭ analiz dannykh po fragmentatsii khromatina nukleazami.