Identification and control of synthesis of the dsdC activator protein.
Operon fusions between the D-serine deaminase regulatory and structural genes and lacZ were constructed and used to examine the control of expression of the positive regulatory gene, dsdC. Merodiploid strains containing both dsdCp::Mu d (lac Apr) and dsdC+A+ produced only one-fourth as much beta-galactosidase as did the haploid dsdCp::Mu d (lac Apr) strains, indicating that the dsdC+ product repressed its own synthesis. The repression was reversed by D-serine. dsdC expression was not depressed in a cya background. The basal level of D-serine deaminase was the same in wild-type and dsdCp fusion strains. The dsdC gene product was identified in maxicell strains harboring dsd plasmids as a 34,000-dalton protein. dsdC gene transcription proceeded clockwise; thus, its promoter is adjacent to that of dsdA.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D000242:Cyclic AMP / Q000378:metabolism; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D012695:L-Serine Dehydratase / Q000378:metabolism; D012694:Serine / Q000494:pharmacology; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D012694:Serine; D000242:Cyclic AMP; D012695:L-Serine Dehydratase