The construction of cosmid libraries of eukaryotic DNA using the Homer series of vectors.
We have constructed and characterised a series of approved, disabled cosmid vectors which we call Homer cosmids and have examined the optimal conditions for the construction of libraries of eukaryotic DNA segments using these vectors. Analysis of these libraries shows that most of the sequences we have tested for are present at the expected frequency and that the libraries can be stably propagated. We have also directly tested the stability of cosmid clones carrying tandemly repeated inserts. This work shows that it should be possible to clone most eukaryotic genes using cosmid vectors and that such cloning systems have considerable advantages over those more commonly used.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000235:genetics
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D012335:RNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes