The need of protein synthesis for the effect of LH--RH on the cAMP level in the anterior pituitary in vitro.
The effect of LH--RH on cAMP accumulation in the anterior pituitary of male rats was investigated. The effect consisted of two phases. In the first phase of incubation (about 2 hours), there was no change in the cAMP level, while in the second phase a significant increase was observed. Cycloheximide (10(-5)M) given simultaneously with the LH--RH totally blocked the observed effect of LH--RH. If cycloheximide was given in the second phase of incubation, when the cAMP level was already elevated, its further rise was prevented and the elevated cAMP level remained unchanged for 2 or more hours. Actinomycin D (10(-5)M) added together with LH--RH totally abolished the action of the latter, but if actinomycin D was given 1 hour after LH--RH a significant increase of the cAMP level was found at the end of the 4-hour incubation.
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009419:Nerve Tissue Proteins; D007987:Gonadotropin-Releasing Hormone; D003513:Cycloheximide; D000242:Cyclic AMP