Simultaneous isolation of bovine eosinophils and neutrophils on gradients of Percoll.
Bovine eosinophils and neutrophils have been isolated from venous blood using a two-step procedure which involves initial preparation of a granulocyte-rich fraction by centrifugation and osmotic shock followed by sensity gradient  centrifugation on Percoll. Good yields of both cell types are obtained with purities of 90% or better. The isolated cells are viable and actively phagocytic. The effects of phagocytosis on the metabolism of carbon-1 of d-glucose and the incorporation of iodine have been measured and several differences between bovine cells and those of other species were noted.
D000818:Animals; D002417:Cattle; D002469:Cell Separation; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D004804:Eosinophils; D007111:Immunity, Cellular; D009504:Neutrophils; D010587:Phagocytosis; D011205:Povidone / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*; D013997:Time Factors
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone