Synthesis of human beta-endorphinyl-thiolglycine and its use for the preparation of affinity columns of beta-endorphin.
The peptide human beta-endorphinyl-thiolglycine (I) has been synthesized by the solid-phase method. The citraconyl derivative of peptide I was coupled to aminohexyl-Sepharose by reaction with silver nitrate/N-hydroxysuccinimide in water. The citraconyl groups were removed in aqueous acetic acid and the resulting resin was used in affinity chromatography for the purification of antisera to beta-lipotropin and beta-endorphin.
D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D004723:Endorphins / Q000138:chemical synthesis*; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D011863:Radioimmunoassay; D001615:beta-Endorphin / Q000031:analogs & derivatives
D000596:Amino Acids; D004723:Endorphins; D007202:Indicators and Reagents; D001615:beta-Endorphin; C036781:beta-endorphinyl-thioglycine