Simple apparatus for collecting estuarine sediments and suspended solids to detect solids-associated virus.
Laboratory trials of a new sampler for collection of estuarine sediment-associated virus resulted in a recovery effectiveness averaging 30% for two enteroviruses and rotavirus SA11. A minimal recovery potential of 54% was calculated when losses caused by virus concentration procedure inadequacies were excluded. Both sediment-associated and suspended solids-associated viruses were collected with the sampler. Recoveries of 61 and 60% poliovirus and rotavirus, respectively, were obtained from salt water-suspended, solids-associated virus. The unique advantage of the sampler for selective collection of virus-associated top layers of sediment, plus collection over extensive areas, resulted in recovery of more virus than was obtained with a commonly used dredge-type sampler.
D004770:Enterovirus / Q000302:isolation & purification; D008828:Microbiological Techniques / Q000295:instrumentation*; D017955:Poliovirus / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification; D012988:Soil Microbiology; D014780:Viruses / Q000302:isolation & purification*; D014871:Water Microbiology