Interaction of Herpes simplex virus with human cell lines at various stages of lymphoid differentiation.
The growth of herpes simplex virus (HSV) type 1 in human lymphoid cell lines, arrested at various stages of differentiation, was studied. The synthesis of viral DNA and antigens and the production of infectious virus were followed in null, B and T leukemia-lymphoma cell lines. It was found that while "nondifferentiated" null cells and cells differentiating along the B pathway, even at very early stages of differentiation, supported HSV growth, cell lines of T origin at all stages of differentiation were generally nonpermissive. The failure of T cells to support the growth of HSV did not result from inefficient adsorption to the host cells. However, viral DNA synthesis was not detected, and a significant reduction in the ability of synthesize HSV antigens was observed. It is suggested that the block in the growth cycle of the virus occurs at a stage after adsorption of the virion to the cells, but prior to synthesis of its DNA and proteins.
D001402:B-Lymphocytes / Q000382:microbiology; D002454:Cell Differentiation; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D008215:Lymphocytes, Null / Q000382:microbiology; D018139:Simplexvirus / Q000254:growth & development*; D013601:T-Lymphocytes / Q000382:microbiology; D014776:Virus Cultivation
D004279:DNA, Viral