Transport of 6-deoxy-D-glucose and D-xylose by untransformed and SV40-transformed 3T3 cells.
Transport rates of the nonphosphorylated D-glucose analogs 6-deoxy-D-glucose and D-xylose were measured in quiescent and serum-stimulated cultures of mouse 3T3 cells, in SV40-transformed 3T3 cells (SV101), and in a density revertant cell line derived from SV101 (FI-SV101). Initial rates of both entry and exit of 6-deoxy-D-glucose and D-xylose were more than threefold higher in serum-stimulated 3T3 and in SV101 cells than they were in quiescent 3T3 cells, but transport rates were not higher in the transformed cells (SV101) than they were in serum-stimulated 3T3. Confluent cultures of FI-SV101 showed lower rates of transport than serum-stimulated FI-SV101, but not as low as quiescent 3T3 cells enter the quiescent G0 state, but emphasize that SV40-transformed 3T3 cells do not show higher activity of the D-glucose carrier than do actively growing 3T3 cells. Thus, enhanced glucose transport appears not to be a specific consequence of transformation, but a reflection of the active growth state of the cell.
D000818:Animals; D001692:Biological Transport; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D003470:Culture Media; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D013539:Simian virus 40; D014994:Xylose / Q000378:metabolism*
D003470:Culture Media; D003837:Deoxy Sugars; D003847:Deoxyglucose; D014994:Xylose