title: Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.
abstract: Overlapping cDNA clones representing the entire genome of poliovirus type 3 have been prepared in E. coli by two separate methods. Cloning of RNA . cDNA hybrids produced a more comprehensive set of clones with generally larger cDNA inserts than cloning of double - stranded cDNA. A restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3' terminus, comprising the region encoding the protease and polymerase proteins, are presented.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D009713:Nucleotidyltransferases / Q000235:genetics*; D010447:Peptide Hydrolases / Q000235:genetics*; D010957:Plasmids; D017955:Poliovirus / Q000201:enzymology* / Q000235:genetics
chemical_list: D004247:DNA; D009713:Nucleotidyltransferases; D004262:DNA Restriction Enzymes; D010447:Peptide Hydrolases