Tissue-specific distribution of DNA-binding nuclear proteins.
The DNA-binding capacity of nuclear proteins of mouse cells was examined by the protein-blotting method. Under conditions in which the lac repressor specifically binds to the lac operator, the DNA-binding nuclear proteins from different tissues showed a tissue-specific distribution, suggesting that the species and amounts of nuclear proteins with DNA binding activity differ in different tissues. When cloned eukaryotic genes were used for binding, eukaryotic DNA showed stronger binding than prokaryotic DNA. Competition experiments suggested that many nuclear proteins have different DNA binding properties from that of the prokaryotic repressor.
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005796:Genes; D005914:Globins / Q000235:genetics; D007763:Lac Operon; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000378:metabolism; D014018:Tissue Distribution
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D005914:Globins; D004265:DNA Helicases