title: Effect of leishmanial excreted factor on the activities of adenylate cyclase from hamster liver and Leishmania tropica.
abstract: The carbohydrate segment of the excreted factor of culture cells from L. tropica and L. donovani was shown to interact with the adenylate cyclase from hamster liver. At a concentration of 1 mg/ml the excreted factor inhibited the stimulated activity of the mammalian enzyme by about 50%, independent of the activation by glucagon, 5'-guanylylimidodiphosphate and forskolin, respectively. In contrast to the adenylate cyclase system from hamster liver the enzyme from L. tropica-culture cells was not stimulated by addition of glucagon, 5'-guanylylimidodiphosphate and forskolin. In addition, the activity of the adenylate cyclase from L. tropica was not affected by addition of its endogenous excreted factor.
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D007891:Leishmania / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D000242:Cyclic AMP; D000262:Adenylyl Cyclases