Effects of some m-cholinomimetics and alpha- and beta-adrenomimetics on cyclic-AMP content of the rat submaxillary gland.
The experiments were performed on four groups of rats of both sexes, anesthetized with pentobarbital: one control and three experimental groups. The experimental group was treated with carbachol 50 micrograms/100 g, isoprenaline 10 micrograms/100 g and phenylephrine 10 micrograms/100 g, respectively. The cyclic AMP was assayed by a R.I.A. method, at 4-6 min after administration of the drugs. The salivary secretion was estimated semiquantitatively by qualifying adjectives: very copious, copious and absent. In the control group the concentration of cAMP was 699 +/- 212 p.mol/g of wet tissue; carbachol administration resulted in an important and statistically significant increase (1030 +/- 205 p.mol/g; P less than 0.001) and isoprenaline increased the cAMP concentration more than twice (1414 +/- 368 p.mol/g, P less than 0.001). Phenylephrine decreased the level of cAMP to 481 +/- 79 p.mol/g, the decrease being statistically significant (0.02 less than p less than 0.05).
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis*; D007545:Isoproterenol / Q000494:pharmacology*; D010277:Parasympathomimetics / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects*; D013566:Sympathomimetics / Q000494:pharmacology
D010277:Parasympathomimetics; D013566:Sympathomimetics; D010656:Phenylephrine; D002217:Carbachol; D000242:Cyclic AMP; D007545:Isoproterenol