Organization of proviral DNA and protein composition of hormonally stimulated C57Bl/10 strain-associated mouse mammary tumor virus.
Two continuous lines, BL-MaTU/A1 and BL-MaTU/s6, were established from C57Bl/10 mammary adenocarcinomas induced by DMBA-prolactin-estradiol treatment. Under in vivo stimulation with dexamethasone, insulin, prolactin, and prostaglandin A1, the cells produce detectable amounts of B-type particles with biochemical properties similar to the GR-MuMTV. Analysis of restriction endonuclease-generated fragments of cellular DNA revealed identical patterns in the integration sites and internal recognition sites of MuMTV proviral equivalents in the tumor cells and normal organs of C57Bl/10 strain mice. The restriction DNA fragments of C57Bl/10-associated MuMTV proviral DNA are closely related to those of the Balb/c-associated MuMTV. These results indicate the endogenous origin of MuMTV produced in the hormonally stimulated cultures of DMBA-prolactin-estradiol-induced C57Bl/10 mammary tumors.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005814:Genes, Viral; D006728:Hormones / Q000494:pharmacology*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000382:microbiology* / Q000473:pathology; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D014764:Viral Proteins / Q000032:analysis*
D004279:DNA, Viral; D006728:Hormones; D014764:Viral Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene