Clonal origin of the Philadelphia translocation in chronic myeloid leukemia demonstrated in somatic cell hybrids using an adenylate kinase-1 polymorphism.
Hybrid cell lines were derived from fusion of rodent cells with leukocytes from a t(9q+; 22q-)-positive chronic myeloid leukemia (CML) patient carrying a chromosome No. 9-linked adenylate kinase-1 (AK1) polymorphism (AK1 1-2). The AK1*2 allele was consistently expressed when 9q+ was present, whereas the AK1*1-coded isozyme was formed when the normal chromosome No. 9 was present. These results provide additional data confirming the clonal origin of the Ph1 translocation in CML.
D000263:Adenylate Kinase / Q000235:genetics*; D000818:Animals; D002904:Chromosomes, Human, 21-22 and Y; D002906:Chromosomes, Human, 6-12 and X; D002999:Clone Cells; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells; D007951:Leukemia, Myeloid / Q000235:genetics*; D051379:Mice; D010770:Phosphotransferases / Q000235:genetics*; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic
D010770:Phosphotransferases; D000263:Adenylate Kinase