Evidence for a bladder cell glycolipid receptor for Escherichia coli and the effect of neuraminic acid and colominic acid on adherence.
The rat bladder epithelial cell receptors involved in mannose-sensitive adherence of Escherichia coli strains were studied. Sodium metaperiodate and lipase pretreatment of epithelial cells significantly reduced bacterial adherence to cells whereas trypsin and phospholipase C had a marginal or insignificant effect on adherence. Neuraminidase and colominic acid significantly increased adherence, whereas N-acetylneuraminic acid significantly reduced adherence. These data suggest that the rat bladder epithelial cell receptors involved in mannose-sensitive adherence are glycolipids. In addition, the data suggested that sialic acid on bladder epithelial cells acts as a nonspecific inhibitor of adherence, whereas colominic acid, a component of some E. coli K1 capsules, may act as a promoter of adherence.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D004926:Escherichia coli / Q000502:physiology*; D005260:Female; D006017:Glycolipids / Q000502:physiology*; D009438:Neuraminic Acids / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000187:drug effects*; D001743:Urinary Bladder / Q000382:microbiology*
D006017:Glycolipids; D009438:Neuraminic Acids; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C033639:glycolipid receptor; D010504:Periodic Acid; C014420:colominic acid