Structure of the Tetrahymena pyriformis rRNA gene. Nucleotide sequence of the transcription termination region.
The 3'-terminal regions of the 25 S rRNA and 35 S rRNA were mapped in the HindIIIC fragment of the T. pyriformis rRNA gene (Niles, E. G., and Jain, R. K. (1981) Biochemistry 20, 905-909). The precise locations of these termini were identified by S1 nuclease mapping. The 35 S rRNA was shown to contain two major 3'-ends, one identical to the 3'-end of the mature 25 S rRNA and one which is 15 bases longer. The nucleotide sequence of 440 base pairs of DNA in the terminator region was determined. There are several oligo T stretches and regions of dyad symmetry. Three models of transcription termination are presented which are based on possible secondary structures in the 3'-terminal region of the 35 S rRNA.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000235:genetics*; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes