Determination of vitamin D3 in cod-liver oil by high-performance liquid chromatography.
The present British Pharmacopoeia monograph for cod-liver oil requires a bioassay for the vitamin D3 content which is both time-consuming and complex. Alternative assays employing chromatographic procedures have been described but all these involve prior saponification of the oil. A selective extraction for vitamin D3 without the need for saponification is reported in this paper. The extraction utilizes only chromatographic assay using argentation on reversed-phase silica, with vitamin D2 as the internal standard. Reproducibility of injection gave a coefficient of variation of 0.6%, and repeatability of extraction for six samples gave a coefficient of variation of 6.8%.
D000818:Animals; D002762:Cholecalciferol / Q000302:isolation & purification* / Q000592:standards; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D003060:Cod Liver Oil / Q000032:analysis*; D005395:Fish Oils / Q000032:analysis*; D005399:Fishes; D012835:Silver Nitrate
D005395:Fish Oils; D002762:Cholecalciferol; D003060:Cod Liver Oil; D012835:Silver Nitrate