Comparisons of dose-response data for various standard and recombinant DNA-derived human interferons.
Dose responses of the NIH standard IFN-alpha and IFN-beta preparations were compared with recombinant DNA-derived IFN-beta (IFN-beta 1) and various IFN-alpha subtypes, molecular hybrids and mixtures. Cytopathic effect assays were employed using vesicular stomatitis virus on human HeLa and bovine MDBK cells. A natural peripheral blood lymphocyte and recombinant DNA-derived IFN-gamma were also included in the comparisons. Two-tailed t-tests between slopes showed no significant differences in any pair-wise comparison using crude or highly purified preparations.
D000818:Animals; D002417:Cattle; D003588:Cytopathogenic Effect, Viral; D004274:DNA, Recombinant; D004306:Dose-Response Relationship, Immunologic; D006367:HeLa Cells; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage* / Q000302:isolation & purification; D012015:Reference Standards; D014721:Vesicular stomatitis Indiana virus
D004274:DNA, Recombinant; D007370:Interferon Type I