[Study of the DNA of the causative agent of whooping cough, Bordetella pertussis].
A number of physico-chemical properties of Bordetella pertussis DNA has been studied. The values of its floating density and melting point have been established, which has allowed one to calculate the GC composition of B. pertussis DNA. The average molecular weights of the fragments of B. pertussis DNA, resulting from its hydrolysis with specific endonucleases EcoR, BamH 1, Sal 1, Pst 1, have been determined, and thus the basis has been provided for establishing the number of clones necessary for obtaining the complete lset of B. pertussis genes.
D001886:Bordetella pertussis / Q000032:analysis* / Q000235:genetics; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight
D004269:DNA, Bacterial
Izuchenie DNK vozbuditelia kokliusha Bordetella pertussis.