Cytoplasmic RNA sequences complementary to cloned chick delta-crystallin cDNA show size heterogeneity.
Double-stranded complementary DNA (cDNA) sequences were prepared from day-old chick lens total polysomal RNA and inserted into the unique PstI restriction site of the plasmid pBR322. Colonies containing sequences complementary to abundant lens poly(A)-containing RNA sequences were identified by using lens 32P-labelled cDNA. Some of these clones have been characterized as containing delta-crystallin mRNA coding sequences by genomic DNA blot hybridization and RNA blot hybridizations. Hybridization of labelled DNA from such clones to RNA blots detected four size classes of delta-crystallin RNA sequences, although Southern blots indicated that there are probably only two delta-crystallin genes.
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D003459:Crystallins; D003593:Cytoplasm / Q000032:analysis; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004586:Electrophoresis; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic
D003459:Crystallins; D012335:RNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes