[Cytochemical detection and cytofluorimetric analysis of alkaline phosphatase in continuous lines of thymic lymphomas induced in mice by a radiation leukemia virus].
Alkaline phosphatase was studied in cell lines established from Radiation Leukemia Virus induced thymic lymphomas of C57BL/Ka mice. The cytochemical staining techniques and flow cytofluorimetry analysis described by Dolbeare et al. (J. Histochem. Cytochem., 1980, 28, 419-426) were used. The alkaline phosphatase found in lymphoma cells was heat labile and L-homoarginine. L-phenylalanine and p-bromotetramisole sensitive and is probably similar to the isoenzyme present in mouse placenta, kidney and liver. Very little alkaline phosphatase activity was detected in normal thymus of adult mice, suggesting that the method used in the paper could be helpful for studying the emergence of the first neoplastic cells during the leukemogenic process.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D006651:Histocytochemistry; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000201:enzymology*; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010649:Phenylalanine / Q000494:pharmacology
D010649:Phenylalanine; D000469:Alkaline Phosphatase
Détection cytochimique et analyse cytofluorimétrique des phosphatases alcalines dans des lignées continues de lymphomes thymiques induits chez la Souris par le virus des radioleucoses.