Expression of murine H-2Kb histocompatibility antigen in cells transformed with cloned H-2 genes.
Cosmids containing H-2 histocompatibility antigen genes of the H-2b haplotype have been isolated. One of these genes expresses a 45,000 molecular weight protein, indistinguishable from H-2Kb when introduced into mouse L cells. These H-2Kb transformed L cells can be killed by allospecific anti-H-2Kb cytotoxic T cells. Moreover, when infected with influenza virus, they can be killed by an H-2Kb-restricted, influenza virus-specific cytotoxic T cell line. These results show that expression of the H-2Kb gene product on the L-cell surface is sufficient to make it a target for specific T-cell killing.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D003602:Cytotoxicity, Immunologic; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D007739:L Cells; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D014170:Transformation, Genetic
D000954:Antigens, Surface; D000956:Antigens, Viral; D004274:DNA, Recombinant; D006183:H-2 Antigens; D004262:DNA Restriction Enzymes