title: Location of the single gene for elongation factor Tu on the Euglena gracilis chloroplast chromosome.
abstract: The structural gene for elongation factor Tu (EF-Tu) has been mapped by heterologous hybridization to a 2900 base pair sequence of Euglena gracilis Klebs Strain Z Pringsheim chloroplast DNA within the EcoRI fragment, Eco N. The hybridization probes were obtained from a HhaI restriction fragment containing internal sequences to Escherichia coli EF-Tu, located in the tufA gene locus, and from an EcoRI restriction fragment of chloroplast DNA from the eukaryotic algae Chlamydomonas reinhardii, containing the 3' end of the chloroplast EF-Tu gene. This is the second identified protein gene locus to be mapped on the E. gracilis chloroplast genome, and the first using prokaryotic DNA as a probe.
mesh_terms: D002736:Chloroplasts / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D005056:Euglena gracilis / Q000235:genetics*; D005796:Genes; D009693:Nucleic Acid Hybridization; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D010445:Peptide Elongation Factors; D004262:DNA Restriction Enzymes; D010444:Peptide Elongation Factor Tu