title: Adenylyl cyclase and protein carboxyl methylase in human spermatozoa.
abstract: Adenylyl cyclase (AC) and protein carboxyl methylase (PCM) activities in human ejaculated spermatozoa were measured in 31 men being evaluated for infertility at the Infertility Clinic. The spermatozoal Mn2+-sensitive AC activities ranging between zero and 1.4 nmol cyclic adenosine monophosphate (cAMP)/10(9) cells/min, was positively correlated with motility index [% motile sperm X degree (0-4)] (r = 0.72, rho less than 0.05). This confirms a role for cAMP in human sperm motility. PCM activities, ranging between zero and 160 pmoles (CH3OH/10(9) cells/min), were significantly correlated with AC activities (r = 0.86, p less than 0.001). However, PCM activities were not positively correlated with motility index (p = 0.065). PCM most probably is a factor involved in spermatozoal motility since two patients suffering necrospermia possessed spermatozoa deficient in both AC and PCM activities.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000328:Adult; D000242:Cyclic AMP / Q000032:analysis; D006801:Humans; D007248:Infertility, Male / Q000201:enzymology; D008297:Male; D008345:Manganese / Q000378:metabolism; D008875:Middle Aged; D011496:Protein Methyltransferases / Q000378:metabolism*; D011497:Protein O-Methyltransferase / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology*
chemical_list: D008345:Manganese; D000242:Cyclic AMP; D011496:Protein Methyltransferases; D011497:Protein O-Methyltransferase; D000262:Adenylyl Cyclases