Hydrodynamic characterization of the Triton X-100 solubilized lactogenic hormone receptor of rat liver.
Rat liver plasma membranes, prelabeled with radioactive human growth hormone, were extracted with Triton X-100. The solubilized lactogenic hormone receptor had a Stokes radius of 54.2 A on Sepharose 6B chromatography. Numerical integration of the results of sedimentation experiments on the solubilized hormone-receptor-Triton X-100 complex in sucrose/H2O and sucrose/2H2O density gradients produced an s20,w = 5.05 X 10(-13) S and a partial specific volume of 0.791 cm3 g-1. From these data a molecular weight of 148 000 and frictional ratio of 1.40 for the hormone-receptor-Triton X-100 complex were calculated. Triton X-100 was calculated to comprise 32.4% of the complex, and thus, the hormone-receptor complex has a molecular weight of 99 800. These results indicate that the lactogenic hormone receptor, as would be expected of an integral membrane protein, has the capacity to bind a large amount of detergent.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D011981:Receptors, Prolactin; D012995:Solubility
D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011092:Polyethylene Glycols; D011388:Prolactin; D017830:Octoxynol