title: Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNa-binding protein.
abstract: The major herpes simplex virus type 2 DNA-binding infected cell-specific polypeptides 11 and 12 have been purified to homogeneity from extracts of virus-infected cells. Monospecific antiserum to the purified protein has been made and used to examine virus temperature-sensitive mutants for defects in the synthesis of the protein and to probe virus DNA synthesis in isolated chromatin. The purified protein acted directly on a polydeoxyadenylic acid-polydeoxythymidylic acid helix, reducing its melting temperature. The results indicated that the protein functions in virus DNA synthesis.
mesh_terms: D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D004247:DNA / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D004268:DNA-Binding Proteins; D007106:Immune Sera; D009691:Nucleic Acid Denaturation; D011067:Poly dA-dT; D018139:Simplexvirus / Q000032:analysis*; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D007106:Immune Sera; D014764:Viral Proteins; D011067:Poly dA-dT; D004247:DNA