title: Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis.
abstract: The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids deleted for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D004261:DNA Replication; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008970:Molecular Weight; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements