DNA mediated gene transfer using simian virus 40 or polyoma virus morphological transformation as selective markers.
The stable uptake of a bacterial plasmid by mouse or rat cells has been detected following the respective co-transfection of these cells with plasmid in the presence of either Simian virus 40 (SV40) or Polyoma virus DNAs. About 60-70 percent of the resulting SV40-transformed mouse cell clones and about 40 percent of the Polyoma transformed rat cell clones contained plasmid DNA sequences.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D051379:Mice; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics; D014162:Transfection; D014170:Transformation, Genetic