Structural and biosynthetic studies on the two molecular forms of the (Na+ + K+)-activated adenosine triphosphatase large subunit in Artemia salina Nauplii.
The large subunit of (Na+ + K+)-activated ATPase from brine shrimp, Artemia salina, migrates as two bands in sodium dodecyl sulfate-polyacrylamide gels. The slower migrating band, as observed in neutral or alkaline gel systems, is designated alpha 1 and the faster, alpha 2. Structural and biosynthetic studies have been performed to determine if these two bands represent independent molecular forms or precursor products. Peptide mapping of partial proteolytic digests of alpha 1 and alpha 2 showed no distinguishable difference between them whereas this technique produced very distinct differences in the large subunit derived from three different species. The two large subunit bands also behaved identically when cross linked with cupric phenanthroline either in the presence or absence of digitonin, whereas other proteins in these preparations were unaffected. The peptide mapping and cross-linking experiments demonstrate that alpha 1 and alpha 2 have identical or nearly identical primary and probably higher order structure. Their different mobilities may be due to post-translational modification leading, for example, to different oligosaccharide composition. During development of the brine shrimp nauplius, alpha 1 increases in relative abundance while alpha 2 decreases. NaH14CO3 incorporation and pulse-chase experiments indicate that alpha 1 and alpha 2, as well as the small subunit of the brine shrimp (Na+ + K+)-activated ATPase, are synthesized at the same time during development and that all changes in the rates of synthesis of these subunits occur at the same time. The apparent rates of degradation of the subunits are also similar. These results are inconsistent with a precursor-product relationship between alpha 1 and alpha 2.
D000375:Aging; D000818:Animals; D001156:Artemia / Q000254:growth & development*; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification* / Q000378:metabolism
D046911:Macromolecular Substances; D010446:Peptide Fragments; D000254:Sodium-Potassium-Exchanging ATPase