Secretin exerts inhibition of the 8BrcAMP-stimulated 86Rb influx into avian red blood cells.
Avian erythrocytes possess a Na+ and K+ cotransport system which can be inhibited by loop diuretics. The newly discovered diuretic effect of secretin in man led us to study the effect of this hormone on the cotransport system. Secretin caused a 50% inhibition of the 8BrcAMP-stimulated 86Rb influx into red blood cells from goose at a concentration of 8.5 X 10(-6) M, while furosemide and bumetanide caused a 50% inhibition at concentrations of 7 X 10(-6) M and 9 X 10(-8) M, respectively. It is suggested that the diuretic effect of secretin is mediated through an inhibitory or blocking effect on the Na+ and K+ cotransport system.
D015124:8-Bromo Cyclic Adenosine Monophosphate; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D005777:Geese; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology; D012413:Rubidium / Q000097:blood*; D012633:Secretin / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D002034:Bumetanide; D012633:Secretin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D010042:Ouabain; D005665:Furosemide; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium