Studies on the toxicity and binding kinetics of abrin in normal and Epstein Barr virus-transformed lymphocyte culture-I: experimental results - 1.
The effects of treatment with varying doses of abrin, a D-galactose binding lectin, on DNA and protein synthesis of normal and Epstein Barr virus-transformed lymphocytes have been investigated. Activation, stimulation and relative toxicity factor indices are studied as well as possible relationships between the DNA and protein synthesis rates, as measured by simultaneous 3H-TdR and 14C-leucine uptake.
D000036:Abrin / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008934:Mitogens / Q000378:metabolism; D010940:Plant Proteins / Q000633:toxicity*; D014176:Protein Biosynthesis; D013997:Time Factors
D008934:Mitogens; D010940:Plant Proteins; D000036:Abrin; D004247:DNA