title: Nonspecific proteases: a new approach to the isolation of adult cardiocytes.
abstract: The potent collagenolytic activity of nonspecific proteinases suggested their use as enzymatic agents for the dissociation of single adult heart cells. This was assessed in guinea pig hearts perfused for 1 min with solutions containing hyaluronidase (100-10 000 U/mL), trypsin (100-10 000 U/mL), crude collagenase (100-500 U/mL), or nonspecific protease (0.1-100 U/mL). No rod-shaped cells were observed among the cells isolated with these concentrations of hyaluronidase, trypsin, or crude collagenase. By contrast, 45-80% of the cells released with nonspecific protease (5-10 U/mL) were rod shaped and Ca2+ tolerant. Resting and action potentials recorded from cells dispersed with nonspecific protease were similar to those recorded from cells isolated after prolonged collagenase exposure.
mesh_terms: D000200:Action Potentials; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology; D003012:Microbial Collagenase / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology*; D010447:Peptide Hydrolases / Q000494:pharmacology*
chemical_list: D010447:Peptide Hydrolases; D003012:Microbial Collagenase