Identification and determination of 8 alpha, 9 alpha-epoxyhexahydrocannabinol as a metabolite of delta 8-tetrahydrocannabinol of mice in vitro.
1. 8 alpha, 9 alpha-Epoxyhexahydrocannabinol (8 alpha, 9 alpha-EHHC) was formed from delta 8-tetrahydrocannabinol (delta 8-THC) by mouse liver microsomal preparation in the presence of an NADPH-generating system. 2. The epoxide was identified by t.l.c., g.l.c. and g.l.c.-mass spectrometry and, together with 11-hydroxy-delta 8-THC (11-OH-delta 8-THC), was determined by g.l.c. 3. When delta 8-THC was incubated with mouse liver microsomal preparation, 8 alpha, 9 alpha-EHHC and 11-OH-delta 8-THC was formed to the extents of 14% and 23%, respectively, of the added delta 8-THC.
D000818:Animals; D002849:Chromatography, Gas; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*
C019370:1 alpha,2 alpha-epoxyhexahydrocannabinol; D013759:Dronabinol