Stereochemical course of the hydrolysis of thymidine 5'-(4-nitrophenyl [17O,18O]phosphate) in H216O catalyzed by the phosphodiesterase from snake venom.
The phosphodiesterase from snake venom catalyzes the hydrolysis of the Rp diastereomer of thymidine 5'-(4-nitrophenyl [17O,18O]phosphate) in H216O with retention of configuration at phosphorus. This result is in agreement with those previously reported for the hydrolysis of chiral phosphorothioate substrates (Bryant, F. R., and Benkovic, S. J. (1979) Biochemistry 18, 2825-2828; Burgers, P. M. J., Eckstein, F., and Hunneman, D. H. (1979) J. Biol. Chem. 254, 7476-7478). The hydrolysis reaction catalyzed by this enzyme occurs via formation of a covalent nucleotidylated enzyme intermediate.
D000818:Animals; D003435:Crotalid Venoms / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010103:Oxygen Isotopes; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013237:Stereoisomerism; D013938:Thymidine Monophosphate / Q000031:analogs & derivatives; D013942:Thymine Nucleotides
D003435:Crotalid Venoms; D010103:Oxygen Isotopes; D013942:Thymine Nucleotides; C032579:thymidine 5'-4-nitrophenyl phosphate; D013938:Thymidine Monophosphate; D010727:Phosphoric Diester Hydrolases