Activation of glycerophosphocholine phosphodiesterase in rat forebrain by Ca2+.
The highest activity of glycerophosphocholine phosphodiesterase (EC 3.1.4.2) in subcellular fractions of rat forebrain was found in the microsomal fraction though significant amounts were found in fractions containing plasma membranes. With the use of Ca(2+)/EGTA and Ca(2+)/EDTA buffers it was shown that very low concentrations of free Ca(2+) (EC(50)approx. 10(-9)m) could activate the enzyme.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D004533:Egtazic Acid; D004492:Edetic Acid; D010727:Phosphoric Diester Hydrolases; C020677:glycerophosphocholine phosphodiesterase; D002118:Calcium