Cytochalasin inhibition of isolated rat gastric parietal cell function.
Submicrogram concentrations (0.04-0.29 microM) of the microfilament disrupting agents cytochalasins D, E, and B (CD, CE, CB) were shown to inhibit secretagogue-stimulated 14C-aminopyrine accumulation (AP) in isolated rat gastric mucosal parietal cells. The microtubule disrupting agent colchicine had little influence on AP accumulation. Histamine- and dibutyryl cyclic AMP (DbcAMP)-stimulated AP accumulation was inhibited with an order of potency CD greater than CE approximately equal to CB. CB inhibition of these secretagogue actions was, however, only approximately 65-70% of the maximal stimulated response, whereas CD and CE caused 100% inhibition. On the other hand, carbamylcholine-stimulated AP accumulation was inhibited 100% by all cytochalasins tested with an order of potency CD approximately equal to CE greater than CB. These data are discussed in relation to acid secretagogue-induced morphological changes involving actin filament organization in parietal cells.
D000199:Actins / Q000502:physiology; D000818:Animals; D003994:Bucladesine / Q000037:antagonists & inhibitors; D002217:Carbachol / Q000037:antagonists & inhibitors; D003572:Cytochalasins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects*; D006633:Histamine Antagonists; D006863:Hydrogen-Ion Concentration; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D000199:Actins; D003572:Cytochalasins; D006633:Histamine Antagonists; D003994:Bucladesine; D002217:Carbachol