A simple disc technique for the presumptive identification of Legionella pneumophila.
A method is described for the presumptive identification of Legionella pneumophila by the formation of satellite colonies around filter paper discs impregnated with ferric pyrophosphate and L-cysteine hydrochloride on a deficient basal medium. This technique simplifies the differentiation of picked colonies of L pneumophila from other organisms in mixed cultures from environmental and contaminated clinical samples.
D001431:Bacteriological Techniques; D003545:Cysteine; D011756:Diphosphates; D007875:Legionella / Q000302:isolation & purification*
D011756:Diphosphates; D003545:Cysteine