The use of on-line high-performance liquid chromatography-mass spectrometry for the identification of ranitidine and its metabolites in urine.
1. Reverse and normal phase h.p.l.c. systems have been developed for separation ranitidine and three metabolites, desmethylranitidine, ranitidine-S-oxide and ranitidine-N-oxide. 2. These h.p.l.c. systems have been evaluated for characterization of ranitidine and metabolites using an h.p.l.c. coupled to a mass spectrometer with a moving belt interface. 3. Ranitidine and its metabolites were thermally degraded under the conditions required to evaporate the reverse phase eluent that contained 40% aq. 0.05 M ammonium acetate. 4. The normal phase eluent was evaporated in the interface at a lower temp. and satisfactory mass spectra were obtained from 1 microgram of ranitidine and each metabolite injected on to the h.p.l.c. column. 5. Normal phase h.p.l.c.-mass spectrometry has been used to identify ranitidine and three of its metabolites in rabbit and human urine obtained after oral administration of ranitidine.
D000818:Animals; D000897:Anti-Ulcer Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D005260:Female; D005663:Furans / Q000652:urine*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D011817:Rabbits; D011899:Ranitidine
D000897:Anti-Ulcer Agents; D005663:Furans; D011899:Ranitidine