title: Hydroxyl radical production in body fluids. Roles of metal ions, ascorbate and superoxide.
abstract: Hydroxyl radical production, detected by ethylene formation from methional, has been investigated in plasma, lymph and synovial fluid. In the presence of added iron--EDTA, addition of either H2O2 or xanthine and xanthine oxidase gave rise to hydroxyl radical formation that in most cases was not superoxide-dependent. The ascorbate already present in the fluid appeared to participate in the reaction. In the absence of added catalyst, the reaction was hardly detectable, the rate being less than 5% of that observed with 1 microM-iron--EDTA added. This implies that the fluids had little if any capacity to catalyse hydroxyl radical production via this mechanism.
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D005030:Ethylenes / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals; D006801:Humans; D006878:Hydroxides / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D008196:Lymph / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000494:pharmacology; D013582:Synovial Fluid / Q000378:metabolism
chemical_list: D000447:Aldehydes; D005030:Ethylenes; D005296:Ferrous Compounds; D005609:Free Radicals; D006878:Hydroxides; D013438:Sulfhydryl Compounds; C008390:methional; D013481:Superoxides; D004492:Edetic Acid; D001205:Ascorbic Acid