[Interactions of subunits of DNA gyrase].
Interaction of DNA gyrase A- and B-subunits during the process of DNA supercoiling was studied. For this purpose a E. coli Cour-1 mutant resistant to coumermycin and containing a mutation in the B-subunit of DNA gyrase was isolated and the influence of the DNA gyrase A-subunit specific inhibitor-nalidixic acid-on DNA supercoiling by wild-type and mutant enzymes was investigated. It turned out that the enzyme from the Cour-1 mutant strain was more sensitive to nalidixic acid than the DNA gyrase from the wild-type strain. Hence, the mutation affecting the B-subunit is capable to change A-subunit properties. That makes it possible to draw the conclusion about a close structural interaction of DNA gyrase subunits during DNA supercoiling.
D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism*; D004278:DNA, Superhelical; D004926:Escherichia coli / Q000201:enzymology*; D046911:Macromolecular Substances; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology
D004278:DNA, Superhelical; D046911:Macromolecular Substances; D009268:Nalidixic Acid; D004250:DNA Topoisomerases, Type II
Vzaimodeĭstvie sub"edinits DNK-girazy.