Quantitation of herpes simplex virus in vitro and in preocular tear film.
Current methods for virus isolation from preocular tear film are not quantitative. This report presents a sensitive method for detection and quantitation of herpes simplex virus type 1 in unknown samples of small size (less than 50 microliters). Serial tenfold dilutions of stock virus were inoculated onto 16 mm vero cell monolayers, which were monitored for the development of cytopathologic evidence (CPE) for presence of virus and were assigned a severity grade. Standard curves were developed on the basis of time interval between inoculation, recognition of CPE, and CPE score. These curves were used to predict virus titers in unknown samples. This method for virus isolation is simple, efficient, and consistently sensitive to virus titers of less than 10 plaque forming units.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D066298:In Vitro Techniques; D011817:Rabbits; D018139:Simplexvirus / Q000302:isolation & purification*; D013666:Tears / Q000382:microbiology*; D014773:Virology / Q000379:methods*