Binding of benzo[a]pyrene and intracellular transport of a bound electrophilic benzo[a]pyrene metabolite by lipoproteins.
Human serum lipoproteins were isolated by means of size exclusion h.p.l.c. Non-covalent uptake of [3H]benzo[a]pyrene was quantitated for fractions collected from the effluent of a liquid chromatographic separation of human serum, and was found to directly correlate with the lipoprotein concentration. An electrophilic benzo[a]pyrene metabolite, [3H]trans 7,8-dihydrodiol-9,10-epoxybenzo[a]pyrene, non-covalently associated with low density lipoproteins was transferred to human lymphocytes in vitro and bound acid-precipitable nucleic acids of the lymphocytes as a function of time. Benzo[a]pyrene metabolite binding to lymphocyte DNA was demonstrated by means of CsCl density gradient analysis. Non-mitogen-stimulated lymphocytes exposed to very low concentrations of carcinogen in the presence of low density lipoprotein demonstrated [3H]thymidine incorporation; without the concomitant addition of low density lipoprotein the low concentrations of carcinogen did not stimulate [3H]thymidine incorporation.
D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000097:blood; D004261:DNA Replication; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008214:Lymphocytes / Q000378:metabolism; D011485:Protein Binding
D001580:Benzopyrenes; D008074:Lipoproteins; D001564:Benzo(a)pyrene; D004247:DNA