O-glycosidic carbohydrate-peptide linkages of Herpes simplex virus glycoproteins.
Electrophoretically purified HSV-specified glycoproteins with radiolabelled carbohydrates were subjected to mild alkaline borohydride treatment (0.5 M NaOH and 0.5 M NaBH4). The treatment liberated significant amounts of the labelled oligosaccharides. The latter demonstrated molecular weights of about 3,000 as determined by gel filtration. The glycoproteins involved probably belong to the gA/gB complex or gC. The results suggest that HSV specified glycoproteins contain oligosaccharides linked with an O-glycosidic bond to a threonine or serine residue of the polypeptide.
D001894:Borohydrides; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005688:Galactosamine / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D018139:Simplexvirus / Q000032:analysis* / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis*
D001894:Borohydrides; D006023:Glycoproteins; D009844:Oligosaccharides; D014764:Viral Proteins; D005688:Galactosamine; C025364:sodium borohydride; D005944:Glucosamine