Analysis of conjugated estrogens in a vaginal cream formulation by capillary gas chromatography.
A capillary gas-chromatographic method is described for the quantitative analysis of nine equine estrogens in a vaginal cream formulation. The sodium sulfate ethers of the estrogens were selectively extracted from the formulation, subjected to enzyme hydrolysis, and derivatized to their oxime-trimethylsilyl esters. Resolution of the resulting derivatives was achieved on short (15 m) capillary column, wall-coated with cyanopropylmethyl silicon stationary phase.
D002849:Chromatography, Gas / Q000379:methods; D004966:Estrogens, Conjugated (USP) / Q000032:analysis*; D014622:Vaginal Creams, Foams, and Jellies / Q000032:analysis*
D004966:Estrogens, Conjugated (USP); D014622:Vaginal Creams, Foams, and Jellies