title: Adenylate cyclase localization in unfixed specimens of rat oral mucosa and isolated mitochondria.
abstract: The cytochemical localization of adenylate cyclase (E.C.4.6.1.1) was demonstrated in unfixed rat oral mucosal specimens and isolated mitochondrial fractions by use of a modified cytochemical reaction mixture. The adenylate cyclase reaction product was observed in the mitochondrial cristae of intact epithelial and fibroblast cells, and in isolated mitochondria after a 10-min incubation in the reaction mixture. The validity of the cytochemical adenylate cyclase method was supported by biochemical studies.
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis*; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006651:Histocytochemistry; D007854:Lead; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009061:Mouth Mucosa / Q000201:enzymology* / Q000648:ultrastructure; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000201:enzymology
chemical_list: D000266:Adenylyl Imidodiphosphate; D007854:Lead; D000242:Cyclic AMP; D000262:Adenylyl Cyclases