Identification of polypeptide precursors to HSV-1 glycoproteins by cell-free translation.
Using antisera of limited specificity we have detected among the in vitro translation products of HSV-1 RNA, polypeptides antigenically related to infected cell glycoproteins. The results obtained suggest that non-glycosylated polypeptide precursors of mol. wt. 85,000 and 52,000 correspond to infected cell glycoproteins of mol. wt. 120,000 to 126,000 and 56,000 to 68,000 respectively.
D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000096:biosynthesis; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000302:isolation & purification*; D018139:Simplexvirus / Q000032:analysis*; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D006023:Glycoproteins; D011498:Protein Precursors; D014764:Viral Proteins