title: Mutagenic virus replication in human tumor cells.
abstract: Herpes simplex virus was grown in different lines of human tumor and normal cells. The progeny virus was assayed for resistance to iododeoxycytidine, an indicator of a forward mutation in the virus genome. Virus grown in cells from 4 of 5 tumor lines demonstrated greater fractions mutated to iododeoxycytidine resistance than did virus grown in 7 normal human skin cell lines. The data indicate that some lines of human tumor cells modify the herpesvirus replication process, making it more mutagenic. In 2 cases of osteosarcoma patients, normal skin fibroblasts of the patients yielded normal levels of mutagenesis, while their tumor cells gave enhanced mutagenesis.
mesh_terms: D000818:Animals; D001972:Bromodeoxycytidine / Q000031:analogs & derivatives; D002460:Cell Line; D002522:Chlorocebus aethiops; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005910:Glioma; D006801:Humans; D007668:Kidney; D008175:Lung Neoplasms; D008545:Melanoma; D009154:Mutation; D012516:Osteosarcoma; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics*; D012867:Skin; D014779:Virus Replication
chemical_list: D003841:Deoxycytidine; D001972:Bromodeoxycytidine; C011288:ibacitabine