[Isolation and characterization of epithelial cells of colon mucosa: preliminary results].
A procedure for the isolation of epithelial cells from rat and human large bowel is described; the minced tissue was carefully washed with saline and incubated for 45 min in a collagenase-jaluronidase solution; the dispersion of the epithelial cells was achieved by a subsequent treatment with the calcium chelator EDTA. The isolated cells were characterized by cytological and histochemical (PAS, alkaline phosphatase, N-acetyl-D-glucosaminidase) procedures; viability index was assessed by the trypan blue exclusion test; the ability to grow in culture on both liquid and semisolid media was also tested. This method can be successfully applied either to normal or neoplastic colonic mucosa, the resulting cell suspension being suitable for further characterization.
D000818:Animals; D002469:Cell Separation; D003106:Colon / Q000166:cytology*; D004847:Epithelial Cells; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology*; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase
Isolamento e caratterizzazione di cellule epiteliali della mucosa del colon: risultati preliminari.