Use of cryopreserved lymphocytes for the indirect leukocyte migration inhibition assay.
Production of leukocyte migration inhibitory factor (LIF) by fresh and cryopreserved lymphocytes from the same donors was detected by indirect leukocyte migration inhibition (LMI) assay. The same results were obtained when fresh and frozen lymphocytes were tested in parallel. This indicates that cryopreservation does not impair the ability of lymphocytes to produce LIF.
D005615:Freezing; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D008222:Lymphokines / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011309:Preservation, Biological; D014373:Tuberculin / Q000276:immunology
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010835:Phytohemagglutinins; D014373:Tuberculin