Use of a hybrid infectivity assay to analyze primary transcription of temperature-sensitive mutants of the New Jersey serotype of vesicular stomatitis virus.
A hybrid infectivity assay specific for primary transcription was developed to analyze the production of functional mRNAs by vesicular stomatitis virus. A template prepared from wild-type virions of the New Jersey serotype of vesicular stomatitis virus was reconstituted with RNA polymerase proteins from the wild type or temperature-sensitive mutants, and the in vivo temperature sensitivity of the polymerase was determined by infectivity assay. The data demonstrate that the New Jersey temperature-sensitive mutants A1 and E1 have non-temperature-sensitive transcriptases, whereas the B1 and F1 mutants both have temperature-sensitive L proteins which are defective in primary transcription.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005814:Genes, Viral; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012703:Serotyping; D013696:Temperature; D014158:Transcription, Genetic; D018116:Vesiculovirus / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D014764:Viral Proteins / Q000502:physiology
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases