The labeling with 8-azido-cyclic adenosine monophosphate of proteins in vesicles of sarcoplasmic reticulum from rabbit skeletal muscle.
Photoinduced labeling with 8-azido-cyclic AMP of proteins in vesicles from sarcoplasmic reticulum rabbit skeletal muscle has been examined. At concentrations of 0.1 microM or less, specific labeling of three or four bands, representing trace components in the SDS gels, was seen. This labeling was prevented by cyclic AMP. Some of the bands correspond approximately in apparent molecular weight to subunits of cyclic AMP-dependent protein kinases reported in other systems. Attempts to use this reagent as a probe for protein position in the membrane have been unsuccessful since 8-azido-cyclic AMP passes into and through the membrane. The reagent also appears to partition with a linear concentration dependence into the vesicular membrane itself, presumably into the lipid portion.
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001386:Azides; D002850:Chromatography, Gel; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D010539:Permeability; D010782:Photolysis; D011494:Protein Kinases / Q000378:metabolism; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D000345:Affinity Labels; D001386:Azides; D008565:Membrane Proteins; C017042:8-azidoadenosine-3',5'-monophosphate; D000242:Cyclic AMP; D011494:Protein Kinases