Noise analysis of acetylcholine induced current at neuromuscular junctions of the mouse diaphragm.
Miniature end-plate currents (mepcs) and membrane noise elicited by acetylcholine (ACh) iontophoresis were investigated at neuromuscular junctions of the mouse diaphragm. All the experiments were performed at a holding potential of -70 mV at a temperature of 19 degrees C. The equilibrium potential of the ACh response was estimated to be near zero; the mepcs displayed a peak amplitude of 2.46 +/- 0.13 nA (mean +/- S.E.) and relaxed exponentially with a time constant to 1.63 +/- 0.11 msec. Single ACh-activated channels had a conductance of 26.5 +/- 1.5 pS and a mean life time of 1.69 +/0- 0.13 msec.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D003201:Computers; D003964:Diaphragm / Q000294:innervation; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009045:Motor Endplate / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects*; D010791:Phrenic Nerve / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects*
D000109:Acetylcholine