Effect of murine beta-interferon preparation on phagocytosis and cyclic AMP levels in mouse peritoneal macrophages.
Mouse peritoneal macrophages (MPM) cultivated with a mouse beta-interferon preparation (MuIFN-beta) or "mock" IFN were tested for phagocytic ability and intracellular levels of adenosine 3',5'-cyclic monophosphate (cAMP). Suspensions of nonopsonized Escherichia coli (E. coli) were used for phagocytosis experiments. Treatment of MPM with 10(1)-10(3) U per ml of MuIFN-beta stimulated the phagocytic activity and raised the levels of cAMP in MPM. The effect of MuIFN-beta on cAMP levels were dose and time dependent. Maximal cAMP levels were seen when MPM were incubated with 10(3)-10(4) U per ml of MuIFN-beta for five hours. Simultaneous addition of MuIFN-beta and the adenylcyclase inhibitor N-ethylmaleimide to the MPM cultures prevented the rise in cAMP levels but not the increased phagocytic capacity. MuIFN-beta induced enhancement of phagocytosis and elevation of cAMP levels in MPM seem to be two parallel but not interlinked processes.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D000242:Cyclic AMP / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*
D007370:Interferon Type I; D000242:Cyclic AMP; D005033:Ethylmaleimide