title: Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.
abstract: Yeast mitochondria were fractionated into inner membrane, outer membrane, matrix, and intermembrane space. Identity and purity of each fraction were monitored by enzyme assays, dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological detection of characteristic mitochondrial polypeptides. Cytochrome b2 and cytochrome c peroxidase were found to be components of the intermembrane space. The most reliable marker of the outer membrane was a major 29,000-dalton polypeptide component. The availability of submitochondrial fractions provides a basis for studying import of precursor polypeptides into isolated yeast mitochondria.
mesh_terms: D001692:Biological Transport; D002458:Cell Fractionation; D003578:Cytochrome-c Peroxidase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D042844:L-Lactate Dehydrogenase (Cytochrome); D008353:Mannitol / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D010544:Peroxidases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D008353:Mannitol; D007770:L-Lactate Dehydrogenase; D042844:L-Lactate Dehydrogenase (Cytochrome); D010544:Peroxidases; D003578:Cytochrome-c Peroxidase