3-Aminobenzamide synergistically increases sister-chromatid exchanges in cells exposed to methyl methanesulfonate but not to ultraviolet light.
3-Aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis, increased baseline sister-chromatid exchange (SCE) frequencies and acted synergistically with the alkylating agent methyl methanesulfonate to induce exchanges in Chinese hamster ovary and SV40-transformed human (GM637) cells. In contrast, 3-aminobenzamide did not affect the frequency of ultraviolet light-induced SCEs. Our data suggest that, in these 2 cell types, synthesis of poly(ADP-ribose) is more important in damage and repair after exposure to an alkylating agent than after exposure to ultraviolet light.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D010053:Ovary; D013539:Simian virus 40 / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
D001549:Benzamides; C025160:3-aminobenzamide; D008741:Methyl Methanesulfonate