Isolation of an exotic serotype of bluetongue virus from imported cattle in quarantine.
In 1980, 60 zebu cattle from Brazil were admitted into quarantine in Florida for 150 days. During the 30 days between their last test in Brazil and their first test in Florida, four animals developed antibody to bluetongue virus detectable by agar gel immunodiffusion test. Within 62 days after arrival in Florida, three more seroconverted and one more was positive by the 86th day. Virus neutralizing titers of serums from the first four cattle were highest against bluetongue virus serotype 4 and 20; both of these serotypes are exotic to the United States. A bluetongue virus serotype 4 was isolated from one of these animals. The eight positive reactors were slaughtered; the other 52 cattle, which did not develop detectable antibody titers to bluetongue virus, were released into the United States.
D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000302:isolation & purification*; D001938:Brazil; D002417:Cattle / Q000382:microbiology*; D002642:Chick Embryo; D046508:Culture Techniques; D005431:Florida; D005779:Immunodiffusion / Q000662:veterinary; D009500:Neutralization Tests; D011790:Quarantine; D012087:Reoviridae / Q000302:isolation & purification*; D012703:Serotyping / Q000662:veterinary