Possible role of microtubules in tumor cell surface membrane shedding, permeability, and lympholysis.
Low temperature (0 degrees C/1 hr) induced changes in the mastocytoma P815 cell surface morphology which mimicked those induced by alloimmune T-lymphocytes during the cytolytic process. These morphological changes consist of the formation and shedding of membrane vesicles (MV) from the P815 cell surface. When the low-temperature-induced MV shedding process takes place at 22 degrees C, it occurs without changes in membrane permeability to 51Cr, whereas at 37 degrees C, the kinetics of 51Cr release resemble those induced by alloimmune T lymphocytes. The effects of two microtubule-stabilizing agents, deuterium oxide and hexylene glycol, were investigated on the low-temperature-induced MV shedding process as well as on the lymphocyte-mediated cytolysis (LMC) of target cells. These agents were found to inhibit (a) the low-temperature-induced MV shedding, (b) the low-temperature-induced membrane permeability changes to 51Cr, and (c) the LMC process. These data indicate that disruption of microtubules may be involved in all these events.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000473:pathology / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003903:Deuterium / Q000378:metabolism; D017666:Deuterium Oxide; D006018:Glycols / Q000494:pharmacology; D012515:Mast-Cell Sarcoma / Q000276:immunology / Q000473:pathology; D051379:Mice; D008870:Microtubules / Q000276:immunology* / Q000473:pathology / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology; D013696:Temperature; D014867:Water / Q000378:metabolism
D006018:Glycols; D014867:Water; D003903:Deuterium; D017666:Deuterium Oxide; C011480:hexylene glycol