The interaction of miconazole and ketoconazole with lipids.
Staphylococcus aureus can be protected by unsaturated unesterified fatty acids against the growth inhibitory effects of miconazole and ketoconazole observed at concentrations greater than 10(-6) M and greater than 10(-5) M, respectively. Miconazole's fungicidal activity is partly antagonized by oleic acid. However, the effect of ketoconazole on the viability of Candida albicans was not affected by this fatty acid. Cytochrome oxidase and ATPase activities are more sensitive to miconazole (10(-5) M) than to ketoconazole (greater than 10(-4) M) and also liposomes are more susceptible to lysis induced by miconazole. Using differential scanning calorimetry it is shown that high concentrations of miconazole shift the lipid transition temperature of multilamellar vesicles to lower values without affecting the enthalpy of melting. Ketoconazole induces a broadening of the main transition peak only. It is suggested that miconazole changes the lipid organization without binding to the lipids, whereas ketoconazole is localized in the multilayer without having an important direct effect on the lipid organization. The results indicate that miconazole, and to a lesser extent ketoconazole, at doses that can be reached by topical application only, interfere with a third target (the two others are ergosterol synthesis and fatty acid elongation plus desaturation). It is hypothesized that the induced change in lipid organization may play some role in miconazole's topical antibacterial and fungicidal activity, whereas it does not seem to play a significant role in ketoconazole's activities.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D002151:Calorimetry; D002176:Candida albicans / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology; D007093:Imidazoles / Q000037:antagonists & inhibitors*; D007654:Ketoconazole; D008055:Lipids / Q000494:pharmacology*; D008081:Liposomes; D008825:Miconazole / Q000037:antagonists & inhibitors*; D010879:Piperazines / Q000037:antagonists & inhibitors*; D013211:Staphylococcus aureus / Q000187:drug effects; D013347:Subcellular Fractions / Q000201:enzymology
D005227:Fatty Acids; D007093:Imidazoles; D008055:Lipids; D008081:Liposomes; D010879:Piperazines; D008825:Miconazole; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases; D007654:Ketoconazole