Mouse embryonic transforming growth factors related to those isolated from tumor cells.
Growth factors with the two characteristic properties of sarcoma growth factor, the ability to stimulate anchorage-independent growth of normal mouse or rat fibroblasts and the ability to compete with 125I-labeled epidermal growth factor for receptor binding, can be isolated from 12- to 13-day-old normal mouse embryos of various strains. Two size classes of the transforming factor from embryo cells can be isolated with apparent molecular weights of 20,000 and 10,000. The lower-molecular-weight factor has been purified several hundred-fold and has the same properties as the peptides with a molecular weight of 10,000 produced by mouse sarcoma virus-transformed 3T3 cells. Whole-mouse embryos contain approximately 10% as much sarcoma growth factor per g of tissue as do the murine sarcoma virus-transformed cells; how it is distributed among the embryonic tissues remains to be determined.
D000362:Agar; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004622:Embryo, Mammalian / Q000032:analysis* / Q000378:metabolism; D066246:ErbB Receptors; D005260:Female; D005865:Gestational Age; D006133:Growth Substances / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D009053:Sarcoma Viruses, Murine; D014412:Tumor Virus Infections / Q000032:analysis* / Q000378:metabolism
D006133:Growth Substances; D011956:Receptors, Cell Surface; D000362:Agar; D066246:ErbB Receptors