[Some physicochemical properties of native and polymerized glutaraldehyde-treated horse heart cytochrome c].
Glutaraldehyde treatment does not change the absorption of cytochrome c either in the visible or in UV spectra. It brings about the formation of dimers, trimers and high-polymeric forms of cytochrome c and shifts the pI of all cytochrome c isoelectric fractions to more acid pH. Polymerization also results in changes of kinetic parameters of cytochrome c benzidine reaction increasing its affinity to 3,3-diaminobenzidine with a simultaneous decrease in the effectiveness of H2O2 binding. These biochemical changes can be related to immunochemical differences of native and glutaraldehyde-treated cytochrome c.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism*; D005976:Glutaral / Q000494:pharmacology*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*
D000447:Aldehydes; D003574:Cytochrome c Group; D005976:Glutaral
Nekotorye fiziko-khimicheskie svoĭstva nativnogo i polimerizovannogo glutarovym al'degidom tsitokhroma c miokarda loshadi.