Biological activity of anti-thyrotropin anti-idiotypic antibody.
Rabbit anti-rat anti-human thyrotropin anti-idiotypic antibodies have been raised. These antibodies were active at the thyrotropin (TSH) receptor, in that they inhibited 125I-labeled bovine TSH binding to thyroid plasma membranes, stimulated adenylate cyclase activity through a guanyl nucleotide-dependent mechanism, augmented radioiodide transport into isolated porcine thyroid follicular cells and induced such cultured cells to organize into follicles. Aside from substantiating the expectation that anti-hormone anti-idiotypic antibodies may possess properties of the original hormone, this work raised the possibility that thyroid-stimulating antibodies which cause the hyperthyroidism of Graves' disease may be anti-TSH anti-idiotypic antibodies.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007457:Iodine Radioisotopes / Q000378:metabolism; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface; D011989:Receptors, Thyrotropin; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism; D013972:Thyrotropin / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011989:Receptors, Thyrotropin; D013972:Thyrotropin; D000262:Adenylyl Cyclases