Use of monoclonal antibodies to HSV-1 and HSV-2 for serological analysis of the viral glycoproteins.
Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) specify four major glycoproteins designated gA/gB, gC, gD and gE. Type-specific and cross-reacting monoclonal antibodies to the viral glycoproteins have been used to probe their antigenic determinants. Our studies show the following: (i) Type-specific monoclonal antibodies are suitable reagents for serotyping isolates. Discriminating monoclonal antibodies distinguish antigenic variants among the strains of each serotype. (ii) Glycoproteins gA and gB can not be distinguished from each other with a panel of monoclonal antibodies. gA and gB of HSV-2 made in HEp-2 cells contain type specific domains which are not detected in Vero cells. (iii) Electrophoretic mobilities of glycoproteins gA and gB made in HEp-2 cells are significantly less than those made in Vero cells. Lysates of infected Vero cells contain three lower molecular weight polypeptides that also react with monoclonal antibodies directed to gA and gB. (iv) Immunoadsorbant columns constructed from monoclonal antibodies to glycoproteins gC and gD are useful for purification of the glycoproteins from HSV-1 and HSV-2 infected cell extracts.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006561:Herpes Simplex / Q000175:diagnosis; D018139:Simplexvirus / Q000032:analysis* / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014764:Viral Proteins