Evidence for two types of beta-adrenergic-sensitive adenylate cyclase activities in bovine cerebellum.
A latent, as well as an expressed form of adenylate cyclase coupled to beta-adrenergic receptors is present in intact crude synaptosomal preparations from bovine cerebellum. The latent adenylate cyclase activity was assayed in Krebs-Ringer buffer by [3H]adenine labeling and was found to be coupled to a beta 1-like adrenergic receptor. The externally accessible adenylate cyclase assayed in the same medium with [3H]ATP was stimulated via beta 2-adrenergic receptors.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine