Synthesis of immunogenic C-6 derivatives of 2-methoxyestrone and 2-methoxyestradiol-17 beta and characterization of the corresponding antisera.
The synthesis, purification and structural confirmation of the 6-(carboxymethoxyimino) derivatives of 2-methoxyestrone and 2-methoxyestradiol-17 beta are described. These derivatives were coupled to bovine serum albumin by the mixed anhydride method, and rabbits were immunized with the product. The resulting antisera showed high affinity and specificity for 2-methoxyestrone and 2-methoxyestradiol-17 beta, respectively, with low cross reactivities to structurally related estrogens.
D000077584:2-Methoxyestradiol; D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D004966:Estrogens, Conjugated (USP) / Q000138:chemical synthesis*; D004970:Estrone / Q000031:analogs & derivatives*; D006894:Hydroxyestrones / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera; D007202:Indicators and Reagents; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D004966:Estrogens, Conjugated (USP); D006894:Hydroxyestrones; D007106:Immune Sera; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D004970:Estrone; D004958:Estradiol; D000077584:2-Methoxyestradiol; C003655:2-methoxyestrone