Members of the KpnI family of long interspersed repeated sequences join and interrupt alpha-satellite in the monkey genome.
Three different members of a family (KpnI-family) of interspersed repeated DNA sequences were found linked to alpha-satellite sequences in cloned segments of the African green monkey genome. In two of these segments the KpnI-family member is over 6 kbp in length and one of them is flanked by alpha-satellite on both sides indicating that it was inserted into a satellite array. Hybridization of subcloned portions of the family members to restriction endonuclease digests of monkey and human DNA and to a genomic library of African green monkey DNA indicate that 1) family members are interspersed in both the monkey and human genomes, 2) some family members may include sequences in addition to those in the three characterized here, 3) some family members may contain only parts of the sequences characterized here and 4) while the overall organization of the family is similar in the human and monkey genome the majority of the family members in each of the two genomes are distinctly identified by the variant position of certain restriction endonuclease sites. This last observation suggests that within each genome there is a tendency to maintain particular versions of the sequence. Observations 2) and 3) suggest that the KpnI family is complex and includes a variety of subfamilies.
D000818:Animals; D002521:Cercopithecus / Q000235:genetics*; D002522:Chlorocebus aethiops / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004276:DNA, Satellite / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D008099:Liver / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D013154:Spleen / Q000032:analysis
D004274:DNA, Recombinant; D004276:DNA, Satellite; D004262:DNA Restriction Enzymes