Combined radiolabel-binding and immunocytochemical evaluation of receptor-ligand interactions. Studies of transferrin receptors on activated lymphocytes.
A protocol that involved both immunohistological and radiolabel-binding procedures was devised for the study of transferrin-receptor interactions. This composite approach yielded considerably more information than did either technique used alone, and also provided a simple means for exclusion of several common potential sources of error.
D005455:Fluorescent Antibody Technique; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008954:Models, Biological; D011869:Radioligand Assay / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011990:Receptors, Transferrin; D014168:Transferrin / Q000378:metabolism
D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin