Tumor virus induction and host cell capacity inactivation: possible in vitro tests for photosensitizing chemicals.
The responses of two in vitro mammalian virus-host cell systems to the photosensitizing chemicals proflavine sulfate and 8-methoxypsoralen (8-MOP) in the presence of light are described. Infectious simian virus 40 (SV40) could be induced from SV40-transformed hamster cells by treatment with proflavine plus visible light or 8-MOP plus near UV radiation. The same photosensitizing treatments inactivated the capacity of monkey cells to support the growth of herpes simplex virus. SV40 induction and inactivation of host cell capacity for herpesvirus growth might be useful as screening systems for testing the photosensitizing potential of chemicals. Advantages and disadvantages associated with each system are discussed.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D006224:Cricetinae; D000882:Haplorhini; D066298:In Vitro Techniques; D008027:Light / Q000009:adverse effects; D008647:Mesocricetus; D008730:Methoxsalen / Q000633:toxicity; D010787:Photosensitivity Disorders / Q000150:complications*; D011370:Proflavine / Q000633:toxicity; D013539:Simian virus 40; D014412:Tumor Virus Infections / Q000209:etiology*; D014466:Ultraviolet Rays / Q000009:adverse effects; D014775:Virus Activation
D011370:Proflavine; D008730:Methoxsalen