Properties of vitamin E-deficient erythrocytes following peroxidant injury.
Several membrane properties of vitamin E-deficient and normal erythrocytes were studied after incubation of these cells with hydrogen peroxide. Measurements of mean corpuscular volume, cation permeability, membrane Na+, K+ ATPase activity, red cell filterability through 5 mu millipore filters, and membrane protein pattern on sodium dodecyle sulfate-gel electrophoresis revealed marked alterations before lysis. Vitamin E sufficient cells were unaffected by a similar incubation with hydrogen peroxide. We speculate that the changes in membrane function, which follow peroxidant injury, contribute to the shortened red cell survival in the vitamin E deficient state.
D002463:Cell Membrane Permeability / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007234:Infant, Premature; D008565:Membrane Proteins / Q000097:blood; D011188:Potassium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D014811:Vitamin E Deficiency / Q000097:blood*
D008565:Membrane Proteins; D006861:Hydrogen Peroxide; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium