title: Sequence of the chloroplast 16S rRNA gene and its surrounding regions of Chlamydomonas reinhardii.
abstract: The sequence of a 2 kb DNA fragment containing the chloroplast 16S ribosomal RNA gene from Chlamydomonas reinhardii and its flanking regions has been determined. The algal 16S rRNA sequence (1475 nucleotides) and secondary structure are highly related to those found in bacteria and in the chloroplasts of higher plants. In contrast, the flanking regions are very different. In C. reinhardii the 16S rRNA gene is surrounded by AT rich segments of about 180 bases, which are followed by a long stretch of complementary bases separated from each other by 1833 nucleotides. It is likely that these structures play an important role in the folding and processing of the precursor of 16S rRNA. The primary and secondary structures of the binding sites of two ribosomal proteins in the 16SrRNAs of E. coli and C. reinhardii are considerably related.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002696:Chlamydomonas / Q000235:genetics*; D002736:Chloroplasts / Q000378:metabolism*; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal; D005796:Genes; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes