Effect of tumor promoters on soft-agar growth of Swiss 3T3 cells infected with SV40 tsA mutants.
The availability of many SV40 mutants, in which the ability of the virus to transform fibroblasts is variously affected, has prompted us to investigate the effect of treating SV40-infected cells with known tumor promoters on the expression of the transformed phenotype. Using mouse Swiss 3T3 cells and various SV40 tsA mutants unable to transform these cells at 39 degrees C, we have observed a dramatic effect of the potent phorbol ester promoter, 12-O-tetradecanoyl-phorbol-13-acetate, on the formation of macroscopic colonies in soft-agar at the restrictive temperature of 39 degrees C. The efficiency of other phorbol esters and various substances such as anthralin, saccharin, sodium cyclamate, mellitin, griseofulvin and benzoyl peroxide, was in agreement with their reported promoting activities suggesting that mouse Swiss 3T3 cells infected with SV40 tsA mutants could provide a quick and easy test to detect promoters.
D000362:Agar; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002478:Cells, Cultured; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology; D013539:Simian virus 40; D014764:Viral Proteins / Q000378:metabolism
D002273:Carcinogens; D010703:Phorbol Esters; D014764:Viral Proteins; D000362:Agar