[Method of selecting mutations for the plasmid genes of streptomycin resistance in Escherichia coli K-12 cells].
Evidence is presented that streptomycin-dependent mutants of Escherichia coli K-12 are incompatible with the functional activity of streptomycin-resistance genes coding for aminoglycoside adenilyl transferase. On the basis of this phenomenon, a method for selection of streptomycin-sensitive mutations in these genes is proposed, and the possibility of the direct selection of hybrid DNA molecules carrying inserts inactivating streptomycin-resistance genes is discussed. The effectiveness of the method for isolation of streptomycin-sensitive R100.1 plasmid derivatives was demonstrated.
D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D009713:Nucleotidyltransferases; D010641:Phenotype; D010957:Plasmids; D012641:Selection, Genetic; D013307:Streptomycin / Q000037:antagonists & inhibitors*
D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase; D013307:Streptomycin
Metod selektsii mutatsiĭ po plazmidnym genam ustoĭchivosti k streptomitsinu v kletkakh Escherichia coli K-12.