Characteristics of the norepinephrine-stimulated phosphatidylinositol turnover in rat pineal cell dispersions.
Dispersed rat pineal cells can be used for the study of the phosphatidylinositol effect. The response to ( - )-norepinephrine of the incorporation of 32Pi into phospholipids is linear with time and cell concentration, stereospecific, and mediated through alpha-1-adrenergic receptors. Na+ in the incubation medium is obligatory for labeling of phosphatidylinositol and phosphatidylcholine by 32P. In the absence of K+, incorporation of 32P is drastically lowered and no stimulation by norepinephrine occurs. Rb+ can replace K+. Omission of Ca2+ or substitution with Sr2+ preferentially lowers incorporation of radioactivity into phosphatidylcholine. Mg2+ is not required for basal or stimulated labeling.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase; D066298:In Vitro Techniques; D007700:Kinetics; D003012:Microbial Collagenase; D009638:Norepinephrine / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D012964:Sodium / Q000494:pharmacology
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D011942:Receptors, Adrenergic, alpha; D012964:Sodium; D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine