Chromosomal analyses after in vitro fertilization of squirrel monkey (Saimiri sciureus) oocytes.
The effect of 1 microM dibutyril cAMP (cAMP media) on the chromosomal normality of in vitro fertilized squirrel monkey oocytes was examined. A total of 877 oocytes were collected laparoscopically 15-16 h after hCG injection from hormonally-induced follicles of 65 squirrel monkeys. Of these, 330 (37.6%) were prepared between 6 and 30 h after insemination and analyzed. Sperm penetration and the presence of a swollen sperm head in the ooplasm were observed by 6 h, and the nucleate stage by 10 h. Addition of 1 microM dbcAMP did not affect the oocyte maturation rate, however the in vitro fertilization rate was increased 26.5% over controls (46.3 vs. 36.6%). The first cleavage metaphase was found at 16 h after insemination. Between 24 and 30 h post-insemination, 76.6% of fertilized oocytes had reached the first cleavage metaphase. An incidence of triploidy (including dispermy) of 11.7% was observed. After chromosomal analysis of metaphase I, univalents were observed in 16.7% of the cases. Aneuploidy (8.9%) was also found in metaphase II. Of the first cleavage metaphases, 88.0% had the diploid number of chromosomes. Aneuploidy occurred 12.0% of the time and 5 cases of triploidies were observed.
D000818:Animals; D002875:Chromosomes / Q000032:analysis* / Q000502:physiology; D002970:Cleavage Stage, Ovum; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects; D007621:Karyotyping; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D012453:Saimiri; D013997:Time Factors
D000242:Cyclic AMP