Distribution of lipid synthesizing enzymes, 2',3'-cyclic nucleotide 3'-phosphodiesterase, and myelin proteins in rat forebrain subfractions during development.
The distribution of UDP-galactose:ceramide galactosyltransferase (CGalT) was studied in subcellular fractions of rat forebrain during development using zonal centrifugation on linear gradients. Specialized subfractions: SN 1, a microsomal fraction, SN 4, a myelin-related fraction, and purified myelin were also used for this study. For comparison, two microsomal lipid synthesizing enzymes, a myelin-specific enzyme, 2',3'-cyclic nucleotide 3'-phosphodiesterase and myelin proteins were measured in the same subfractions. UDP-glucose:ceramide glucosyltransferase and cerebroside sulfotransferase were confined to microsomes. CGalT was localized in microsomes, but also in myelin and myelin-related fractions. The developmental change in distribution of CGalT in adult animals toward myelin containing fractions could indicate that the replacement of galactosylceramide in compact myelin could be carried out in close proximity to compact myelin (mesaxon, paranodal loops) rather than in the distant oligodendrocyte perikaryon.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000375:Aging; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002458:Cell Fractionation; D002501:Centrifugation, Zonal; D002554:Cerebrosides / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism*; D051147:Ganglioside Galactosyltransferase; D005964:Glucosyltransferases / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008861:Microsomes / Q000201:enzymology; D009185:Myelin Proteins / Q000096:biosynthesis*; D009186:Myelin Sheath / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*
D002554:Cerebrosides; D008055:Lipids; D009185:Myelin Proteins; D005700:Galactosyltransferases; D005964:Glucosyltransferases; D051147:Ganglioside Galactosyltransferase; C023246:ceramide glucosyltransferase; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases