Isolation of virus and antibody containing immune complexes from mink with Aleutian disease by affinity chromatography of equine complement clq.
Affinity chromatography on immobilized equine complement Clq was used for the isolation of complement-binding immune complexes in sera of mink infected with Aleutian disease virus. Immune complexes were isolated and quantitated from 4 of 5 infected mink, as early as 2 weeks after infection and before hypergammaglobulinemia had appeared. The quantity of immunoglobulin G in these immune complexes ranged from 180 to 370 micrograms/ml serum. There were no Clq-binding immune complexes found in mink which were negative for Aleutian disease antibody. Using 125I-labeled BSA-anti-BSA complexes, we demonstrated that the affinity columns bound selectively immune complexes which had formed in antibody excess, whereas immune complexes in antigen excess were not bound. By neutralization of sensitized virus with anti-mink IgG serum, non Clq-binding immune complexes were also detected, which indicates that circulating immune complexes in persistently infected mink are heterogeneous as far as their reactivity with equine Clq is concerned.
D000453:Aleutian Mink Disease / Q000276:immunology*; D000454:Aleutian Mink Disease Virus / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D006736:Horses / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D008907:Mink; D014713:Viruses, Unclassified / Q000276:immunology*
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007074:Immunoglobulin G; D015922:Complement C1q; D003166:Complement Activating Enzymes