Time course of metabolism of benzo[e]pyrene by hamster embryo cells and the effect of chemical modifiers.
In cultures of hamster embryo cells, benzo[a]pyrene (B[a]P) is metabolized primarily in the bay region. In contrast, little or no bay region metabolism of the noncarcinogenic isomer benzo[e]pyrene (B[e]P) could be detected during 12--96-h incubations of hamster embryo cells with 4 microM [3H]B[e]P. The upper limit to 9,10-dihydro-9,10-dihydroxy-B[e]P formation is about 0.2% of the ethyl acetate-soluble metabolites (less than 0.1% of the total metabolites). The major identified metabolites of B[e]P were 4,5-dihydro-4,5-dihydroxy B[e]P and the glucuronide conjugates of 3-OH-B[e]P and 4,5-dihydro-4,5-dihydroxy B[e]P. Simultaneous treatment of cells with either B[a]P or 7,8-benzoflavone (BF) did not induce bay region metabolism of [3H]B[e]P.
D000818:Animals; D001564:Benzo(a)pyrene; D001571:Benzoflavones / Q000494:pharmacology; D001580:Benzopyrenes / Q000378:metabolism* / Q000494:pharmacology; D002273:Carcinogens / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae; D004101:Dihydroxydihydrobenzopyrenes; D004622:Embryo, Mammalian; D007536:Isomerism; D007700:Kinetics
D001571:Benzoflavones; D001580:Benzopyrenes; D002273:Carcinogens; D004101:Dihydroxydihydrobenzopyrenes; C020738:9,10-dihydro-9,10-dihydroxybenzo(a)pyrene; C015813:4,5-dihydroxy-4,5-dihydrobenzo(a)pyrene; D001564:Benzo(a)pyrene; C011512:alpha-naphthoflavone; C026487:benzo(e)pyrene; C021338:3-hydroxybenzo(a)pyrene