title: Percoll density gradient centrifugation. (An improved method for the enrichment of lymphocyte subsets mediating different activities).
abstract: Lymphocyte fractions obtained by Percoll density gradient centrifugation were assayed for their cytotoxic activities both in NK and ADCC cytotoxic systems. Furthermore, the release of a mediator, platelet slowing factor from the same subsets, was evaluated. Different mitogens (phytohemagglutinin, concanavalin A and lipid A) have been used for this purpose. The maximum of cytotoxic activities was recovered in E+ cell-enriched fractions. On the other hand, lipid A was able to stimulate the PSF production from enriched B cells (fraction I); phytohemagglutinin induced the release of PSF from E+ fractions (II and III) and concanavalin A from fraction II only. Our results suggest that Percoll density gradient is a very reproducible method in obtaining lymphocyte subsets enriched in cells mediating either cytotoxic activities or lymphokine release.
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000145:classification*; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone