Inhibition of bone resorption in culture by inhibitors of thiol proteinases.
Leupeptin, antipain, tosyl-lysylchloromethane (Tos-Lys-CH2Cl) and benzyloxy-carbonylphenylalanylalanyldiazomethane (Z-Phe-Ala-CHN2) inhibit reversibly the resorption induced by parathyroid hormone or heparin in cultured mouse bones. Leupeptin and antipain do not affect collagenase production and activity or the enhanced secretion of beta-glucuronidase induced by the bone-resorbing agents. They might thus act by a direct (extracellular?) inhibition of lysosomal thiol proteinases.
D000818:Animals; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000201:enzymology; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases; D005966:Glucuronidase / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D051379:Mice; D003012:Microbial Collagenase / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology; D011480:Protease Inhibitors
D010281:Parathyroid Hormone; D011480:Protease Inhibitors; D006493:Heparin; D005966:Glucuronidase; D003546:Cysteine Endopeptidases; D003012:Microbial Collagenase