Differential effect of prostaglandins and other products of arachidonic acid metabolism on measles virus replication in Vero cells.
The influence of cyclic adenosine 3', 5'-monophosphate (cAMP) and prostaglandin (PG), E1, E2, F2 alpha, A2 and thromboxane B2 (TxB2) and measles virus infection was investigated. Addition of PGE1, E2 and cAMP (10(-3)-10(-)8M) inhibited measles virus replication in Vero cells. TxB2 and PGA2 enhanced replication. Cytotoxic effects were not observed. Inhibition of infectious titers (98%) was most pronounced when agents were present throughout replication. Treated cells exhibited hemadsorbing antigen, but the size and number of cytoplasmic inclusions was decreased by cAMP. Pre-treatment of cells with PGE1 or presence of PGE1 during virus adsorption enhanced infectious titers (30%). Results suggest that cAMP and PGE1 inhibit measles virus production infection. That both enhancing and inhibiting effects of PGs were seen suggest that measles virus may require PG at specific times during the replicative cycle.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D000242:Cyclic AMP / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000382:microbiology; D008459:Measles virus / Q000187:drug effects / Q000502:physiology*; D011453:Prostaglandins / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000494:pharmacology*; D013931:Thromboxanes / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D011453:Prostaglandins; D013931:Thromboxanes; D013929:Thromboxane B2; D000242:Cyclic AMP