Synthesis of human corticotropinyl-thiolglycine and its specific conjugation to bovine serum albumin.
Human corticotropin containing a thiolglycine residue at the carboxyl terminal (I) was synthesized by the solid-phase method. The citraconyl derivative of peptide I was coupled to either a model tetrapeptide or bovine serum albumin by reaction with silver nitrate/N-hydroxysuccinimide in water. The extent of reaction with bovine serum albumin was determined by radioimmunoassay, and the peptide-protein conjugate was shown to possess 12% of the steroidogenic activity of porcine adrenocorticotropin in isolated rat adrenal cells. Peptide I and its conjugate with the model tetrapeptide were fully active in the same system.
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D011485:Protein Binding; D051381:Rats; D012710:Serum Albumin, Bovine; D013256:Steroids / Q000096:biosynthesis; D013329:Structure-Activity Relationship
D000596:Amino Acids; D013256:Steroids; D012710:Serum Albumin, Bovine; C035697:ACTH, thiolglycine-; D000324:Adrenocorticotropic Hormone