Characteristics of plasmalemma alkaline phosphatase of rat mesenteric artery.
General characteristics of alkaline phosphatase activity of the plasma membrane-enriched fraction isolated from rat mesenteric arteries were investigated. The vascular smooth muscle plasmalemma alkaline phosphatase is a metalloenzyme which is strongly inhibited by chelating agents and this inhibition can be completely overcome by addition of Mg2+ or Ca2+. Zn2+ only partially reactivates the enzyme in the presence of low concentrations of EDTA. The enzymatic hydrolysis of p-nitrophenyl phosphate, beta-glycerophosphate, alpha-glycerophosphate, or 3'-adenosine monophosphate showed an optimal activity in the alkaline region between pH 9 and 11. The alkaline phosphatase activity is distinctly different from the plasmalemma ATPase and 5'-nucleotidase activities with respect to their pH dependence, influence by added divalent metal ions and stability against heat inactivation. Vanadate ion, being structurally similar to the transition state analog of the phosphoryl group, potently inhibits alkaline phosphatase with an apparent Ki of 1.5 microM. The altered alkaline phosphatase activity of vascular smooth muscle in relation to its possible physiological function and pathophysiological manifestation associated with hypertensive disease are discussed.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008638:Mesenteric Arteries / Q000201:enzymology / Q000648:ultrastructure; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D014639:Vanadium; D014638:Vanadates; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D015032:Zinc; D002118:Calcium