[Spin probe study of the effect of cholera toxin on enterocyte brush border membranes].
The physical state of the enterocyte brush border membranes during stimulation of cholera diarrhea was examined by electron paramagnetic resonance with the use of spin probes localized in different areas of the lipid bilayer and the spin label covalently bound with protein SH-groups. In vivo experiments have disclosed a slight increase in bilayer fluidity during cholera intoxication. In experiments in vitro the binding of cholera toxin with the enterocyte brush border membranes on addition of 3',5'-AMP (up to 5 X 10(-5) M) did not affect the physical state of the lipid of protein areas of the membrane.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002771:Cholera / Q000378:metabolism; D002772:Cholera Toxin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D049711:History, Modern 1601-; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects*; D008871:Microvilli / Q000187:drug effects*; D011817:Rabbits
D000255:Adenosine Triphosphate; D002772:Cholera Toxin; D000242:Cyclic AMP
Issledovanie metodom spinovogo zonda deĭstviia kholernogo toksina na membrany shchetochnoĭ kaĭmy énterotsitov.