Isolation and properties of beta-endorphin-(1-27)-like peptide from bovine brains.
A beta-endorphin-(1-27)-like peptide was isolated from bovine cerebral hemisphere extracts by gel filtration, ion-exchange chromatography, high performance liquid chromatography and paper electrophoresis. The peptide had tyrosine as the amino-terminal residue and its amino acid composition was nearly identical to that of equine pituitary beta-EP-(1-27). It had also the same mobility as equine pituitary beta-EP-(1-27) in paper electrophoresis. In radioimmunoassay and opiate receptor-binding assay, the brain peptide had 50% activity when compared with human beta-endorphin-(1-27). Evidence for the occurrence of NH2-acetylated form of beta-endorphin-(1-27) is also presented.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006736:Horses; D006801:Humans; D007700:Kinetics; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D010902:Pituitary Gland / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism; D013329:Structure-Activity Relationship; D013570:Synaptic Membranes / Q000378:metabolism; D001615:beta-Endorphin
D004723:Endorphins; D010446:Peptide Fragments; D011957:Receptors, Opioid; D001615:beta-Endorphin; C035581:beta-endorphin (1-27)