[Conductance of cytoplasmic membrane of photoreceptors by the method of intracellular dialysis].
To clear up the effect of agents proposed as a mediator in the phototransduction process on the rod outer segment cytoplasmic membrane a technique based on the intracellular dialysis method was developed. It was shown that in the presence of nucleoside-triphosphates 3',5'-cGMP increased cytoplasmic membrane sodium conductance of dialyzed rod outer segment by 1-4 nS. The dependence of the effect amplitude on the cyclic nucleotides concentrations was obtained. It is suggested that the conductance changes observed are due to the action of cyclic nucleotide dependent protein kinase on the rod outer segment plasma membrane.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D006152:Cyclic GMP / Q000494:pharmacology; D003956:Dialysis / Q000379:methods; D004553:Electric Conductivity; D007700:Kinetics; D010786:Photoreceptor Cells / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism; D011896:Rana temporaria; D012265:Ribonucleotides / Q000494:pharmacology; D012374:Rod Cell Outer Segment / Q000201:enzymology; D012964:Sodium / Q000378:metabolism
D012265:Ribonucleotides; D012964:Sodium; D011494:Protein Kinases; D006152:Cyclic GMP
Izuchenie provodimosti tsitoplazmaticheskoĭ membrany fotoretseptora metodom vnutrikletochnogo dializa.