title: Active and inactive kallikrein released by kidney slices from normotensive and hypertensive rats.
abstract: The relative rates of release of active and inactive kallikrein were investigated in the rat kidney by studying in vitro kidney slices. The slices were kept in Warburg flasks in a Krebs-Ringer solution for 15 up to 60 minutes, and their kallikrein content and the kallikrein-like activity released by them were measured at different times. Renal kallikrein concentration from two-kidney, one clip hypertensive rats was less than in the sham-operated controls (p less than 0.01). Active kallikrein released into the bathing medium was 4.7 +/- 0.3 ng/mg/hr in normotensive rats (n = 10) and 3.3 +/- 0.3 in two-kidney hypertensive animals (n = 12); the difference between both groups was statistically significant (p less than 0.01). Inactive kallikrein is between 60% and 70% of the total kallikrein released by kidneys of normal and hypertensive rats. Thus, under the conditions of these experiments, the kidney slices were able to release a kallikrein-like enzyme in an active and an inactive form. The rate of active kallikrein released from slices of hypertensive animals was lower than in the normotensive controls.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D007610:Kallikreins / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D011506:Proteins; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D007610:Kallikreins