[Aggregation and separability of the shikimate pathway enzymes in yeasts].
Gel filtration was employed to estimate the molecular weights and to determine possible physical aggregation of enzymes [5-dehydroquinate synthase (DHQ synthase), 5-dehydroquinase (DHQase, EC 4.2.1.10), shikimate: NADP oxidoreductase (EC 1.1.1.25), shikimate kinase (EC 2.7.1.71), 3-enolpyruvylshikimate 5-phosphate synthase (EPSP synthase)] in the shikimate pathway in eleven species of yeasts. The five enzymes were not aggregated in extracts of Hansenula henricii, H. fabianii, H. anomala, Candida utilis, Pichia guilliermondii, and Lodderomyces elongisporus. Two enzymes (DHQase and shikimate:NADP oxidoreductase) were not separable by this method and by ion exchange chromatography, and we conclude that they exist as an aggregate in these yeasts. Evidence is presented for an enzyme aggregate containing five activities, with a molecular weight of approximately 280,000 in Rhodosporidium spaerocarpum, Rh. toruloides, Rhodotorula rubra, Saccharomycopsis lipolytica, and Saccharomyces cerevisiae. Similarities between the enzymes in the shikimate pathway of plants, bacteria, and other fungi and those of investigated yeasts are discussed.
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D019883:Alkyl and Aryl Transferases; D002850:Chromatography, Gel; D006836:Hydro-Lyases / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism; D008970:Molecular Weight; D019761:Phosphorus-Oxygen Lyases; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011801:Quinic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism; D013045:Species Specificity; D014166:Transferases / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology*
D011801:Quinic Acid; D012765:Shikimic Acid; D000429:Alcohol Oxidoreductases; C022641:Shikimate dehydrogenase; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase; D008190:Lyases; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase; D019761:Phosphorus-Oxygen Lyases; C023150:5-dehydroquinate synthetase
Aggregation und Trennbarkeit der Enzyme des Shikimat-Pathway bei Hefen.