Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29.
A cosmid clone bank of yeast DNA has been used to isolate the cycloheximide resistance gene cyh2 of Saccharomyces cerevisiae. A cosmid carrying this gene was identified by cross hybridization to another cloned gene, tsm437. The two genes, which are tightly linked genetically are both present on a 31 kb segment of cloned DNA. The cyh2 gene encodes ribosomal protein L29, a component of the large subunit. Blot hybridization analysis reveals that this gene is present as a single copy in the yeast genome, unlike many other yeast ribosomal protein genes which appear to be duplicated. The cyh2 gene also appears to contain an intervening sequence, a characteristic common to most yeast ribosomal protein genes that have been cloned.
D000483:Alleles; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology*; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial; D005796:Genes; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D012269:Ribosomal Proteins; C035078:ribosomal protein L29; D003513:Cycloheximide; D004262:DNA Restriction Enzymes