Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests.
For a long time chelating agents have been known to be inhibitors of alkaline phosphatase activity. However, the use of chelating agents in stopping alkaline phosphatase activity in ELISA reactions is a novel application with several advantages over conventionally used acids or bases.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D003586:Cytomegalovirus Infections / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
D002614:Chelating Agents; D004533:Egtazic Acid; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D003545:Cysteine