Antibody-dependent enhancement of plaque formation on cell lines of macrophage origin - a sensitive assay for antiviral antibody.
An assay for antiviral antibody based on antibody-dependent plaque enhancement (ADPE) in the macrophage cell line P388D1 is described which is as sensitive as, or more sensitive than a radioimmune assay. The method is applicable to a range of Togaviridae and Bunyaviridae although not to Picornaviridae and Herpesviridae. The variables affecting the assay are investigated.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002043:Bunyaviridae / Q000276:immunology; D002460:Cell Line; D006564:Herpesviridae / Q000276:immunology; D008264:Macrophages / Q000382:microbiology*; D008574:Mengovirus / Q000276:immunology; D008722:Methods; D011863:Radioimmunoassay; D014035:Togaviridae / Q000276:immunology; D010948:Viral Plaque Assay; D014779:Virus Replication
D000914:Antibodies, Viral