Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA.
We have developed a 2-dimensional gel method for identification of RNA sequences crosslinked by the intercalative drug 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). This method is being used to localize such sites in E. coli ribosomal RNA. We report here the identification of a site for HMT crosslinking within positions 434 and 497 of 16S rRNA. We suggest a likely site for HMT intercalation, in which residues U548 and U473 become crosslinked via the drug.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D004926:Escherichia coli; D011564:Furocoumarins; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases; D014307:Trioxsalen / Q000031:analogs & derivatives
D011564:Furocoumarins; D012335:RNA, Ribosomal; C016507:hydroxymethyltrioxsalen; D004720:Endonucleases; D012260:Ribonucleases; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic; D014307:Trioxsalen