A fluorometric-high-performance liquid chromatographic assay procedure for several enzymatic activities using formycin analogs of adenosine 5'-mono, 5'-tri, and cyclic 3',5'-monophosphate as substrates.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis*; D000251:Adenosine Triphosphatases / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000032:analysis*; D000903:Antibiotics, Antineoplastic / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004023:Dictyostelium / Q000201:enzymology; D005573:Formycins / Q000138:chemical synthesis*; D007700:Kinetics; D009712:Nucleotides, Cyclic / Q000138:chemical synthesis; D011755:Pyrophosphatases; D012265:Ribonucleotides / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D000903:Antibiotics, Antineoplastic; D005573:Formycins; D009712:Nucleotides, Cyclic; D012265:Ribonucleotides; C021683:formycin 5'-phosphate; C032552:formycin triphosphate; C032553:formycin cyclic 3',5'-monophosphate; D000469:Alkaline Phosphatase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; C019757:ATP pyrophosphohydrolase