title: Secondary structure of Bombyx mori and Dictyostelium discoideum 5S rRNA from S1 nuclease and cobra venom ribonuclease susceptibility, and computer assisted analysis.
abstract: The 5S rRNAs from Bombyx mori and Dictyostelium discoideum were end-labeled with [32-P] at either the 5' or 3' end and sequenced using enzymatic digestion. The secondary structure of these molecules was studied using the single-strand specific S1 nuclease and the base-pair specific cobra venom ribonuclease. Computer analysis of these results was performed and was used to generate a consensus secondary structure for each molecule. A comparison of these results with those of other workers is presented.
mesh_terms: D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D001483:Base Sequence; D012831:Bombyx / Q000032:analysis*; D003201:Computers; D004023:Dictyostelium / Q000032:analysis*; D004546:Elapid Venoms; D004720:Endonucleases; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013045:Species Specificity
chemical_list: D004546:Elapid Venoms; D012335:RNA, Ribosomal; D004720:Endonucleases; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases