HLA antigens on glioma cells from short term cultures.
Biopsied tumor cells from astrocytoma-bearing patients were grown in primary culture for 3-5 days. Both low and high grade tumors were represented in the study. The cultured cells could be shown to express the HLA-A and -B antigens using a multispecific allo-antiserum and a rabbit anti-beta-2 microglobulin antibody. The tumor cells were negative for the HLA-DR determinants when tested with either a rabbit anti-Ia-like antisera or specific anti-HLA-DR allo-antisera. They also failed to stimulate allogeneic lymphocytes in primary mixed lymphocyte-tumor cell cultures but moderately stimulated autologous lymphocytes from the tumor-bearing patient in most of the combinations tested.
D001254:Astrocytoma / Q000276:immunology; D002478:Cells, Cultured; D005909:Glioblastoma / Q000276:immunology; D005910:Glioma / Q000276:immunology*; D006680:HLA Antigens; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed
D006680:HLA Antigens