[Isolation and crystallization of the Clr subunit of the first component of human complement (author's transl)].
Isolation of Clr from Cohn-Fraction I of human plasma is accomplished by a series of steps which include affinity chromatography on IgG-p-azobenzyloxyethylsulfonylethoxy-Sepharose CL-4B, ion exchange chromatography and preparative zone electrophoresis. The protein is easily soluble in 0.1 M Tris/HCl buffer, pH 10.0 and is crystallized readily by means of dialysis against 0.15 M saline, pH 7.0 in the form of hexagonal dipyramides. The structural elucidation using X-ray analysis is now possible.
D002846:Chromatography, Affinity; D003166:Complement Activating Enzymes / Q000302:isolation & purification*; D015923:Complement C1r; D003460:Crystallization; D006801:Humans; D007122:Immunoelectrophoresis
D003166:Complement Activating Enzymes; D015923:Complement C1r
Isolierung und Kristallisation der Human-Komplement-Subkomponente Clr des Cl-Komplexes.