[Cloning the operon genes of riboflavin biosynthesis in Bacillus subtilis on plasmid vector pBR322 in Escherichia coli].
The operon for riboflavine biosynthesis of Bacillus subtilis wild type and its operator-constitutive derivative have been cloned in Escherichia coli cells on the plasmid pBR322 vector. The plasmids constructed were able to transform strains of E. coli and Bac. subtilis from Rib- to Rib+ phenotype. A DNA insert into the EcoRI site of pBR322 causes a decrease in tetracycline gene expression. The operator of the riboflavine operon of Bac. subtilis does not participate in regulation of the operon expression in E. coli cells.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009876:Operon; D010957:Plasmids; D012256:Riboflavin / Q000096:biosynthesis*
D012256:Riboflavin
Klonirovanie genov operona biosinteza riboflavina Bacillus subtilis na plazmidnyĭ vektore pBR322 v Escherichia coli.