title: Presence of an inactive protein immunologically analogous to cytochrome c oxidase in the inner membrane of sweet potato root mitochondria.
abstract: A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively weakly with the mitochondrial inner membrane as compared with cytochrome c oxidase. It exerted no cytochrome c oxidase activity and contained no heme a. The protein was purified by phenyl-Sepharose column chromatography and polyacrylamide gel electrophoresis. The molecular weight of its polypeptide chain was 57,000. In addition, the protein decreased during aging of tissue slices. It is therefore not improbable that the protein is a precursor of cytochrome c oxidase composed of only the subunits of cytoplasmic origin, since aging of tissue slices has been shown to result in an increase in the enzyme activity which is inhibited by chloramphenicol but not by cycloheximide.
mesh_terms: D002458:Cell Fractionation; D002846:Chromatography, Affinity; D003576:Electron Transport Complex IV / Q000276:immunology / Q000378:metabolism*; D004792:Enzyme Precursors / Q000276:immunology / Q000378:metabolism*; D005779:Immunodiffusion; D007425:Intracellular Membranes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D010944:Plants / Q000201:enzymology*; D013367:Submitochondrial Particles / Q000201:enzymology
chemical_list: D004792:Enzyme Precursors; D003576:Electron Transport Complex IV