title: The prevention of an artifact in receptor binding assay by an improved technique.
abstract: The intensity of vacuum suction used during filtration of cerebral membranes on glass fiber filters used in the assays of neurotransmitter receptors is a factor in determining the apparent extent of bound radioactivity. This is not related to protein loss during filtration at various speeds nor to rates of dissociation of various ligand-receptor complexes. It appears to be due to the failure of hydrophobic ligands on dry filters, to be readily removed by aqueous media. A means whereby the vacuum can be held constant is described and shown to eliminate this artifact.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002462:Cell Membrane / Q000032:analysis; D004082:Dihydroalprenolol / Q000378:metabolism; D004088:Dihydroergotoxine / Q000378:metabolism; D008297:Male; D009270:Naloxone / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D013134:Spiperone / Q000378:metabolism; D013331:Strychnine / Q000378:metabolism
chemical_list: D017981:Receptors, Neurotransmitter; D004088:Dihydroergotoxine; D009270:Naloxone; D013134:Spiperone; D004082:Dihydroalprenolol; D011813:Quinuclidinyl Benzilate; D013331:Strychnine