Marker rescue allows direct selection for recombinant plasmids in streptococci.
Resident deletion derivatives (Ems or Cms) of the streptococcal plasmid vector pGB301 rescue antibiotic resistance genes from linearized pGB301 (Emr, Cmr) DNA with high frequency. Insertion of passenger DNA next to an antibiotic resistance determinant of pGB301, which is missing on the resident plasmid, forces corescue of these two plasmid domains, thus allowing direct selection for recombinant plasmids.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010957:Plasmids; D012641:Selection, Genetic; D013291:Streptococcus / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes