Demonstration of C1q - binding to collagen - anticollagen immune complexes in synovial fluids of RA-patients.
Collagen-anticollagen immune complexes were demonstrated indirectly in RA synovial fluids by digestion with collagenase and subsequent antibody determination. Elimination of C1q was performed by affinity chromatography using an anti-C1q column. Binding of C1q to collagen-anticollagen complexes was proven by demonstrating that collagenase-caused liberation of collagen antibodies was abolished or distinctly reduced after passage through the anti-C1q column. Binding of C1q to collagen-anticollagen immune complexes was proven via the fixation of these aggregates onto the anti-C1q column. This was achieved in those 7 RA synovial fluids which contained distinctly demonstrable collagen-anticollagen complexes.
D000906:Antibodies / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000276:immunology*; D003094:Collagen / Q000276:immunology*; D003167:Complement Activation; D003172:Complement C1 / Q000276:immunology; D006801:Humans; D003012:Microbial Collagenase / Q000378:metabolism; D013582:Synovial Fluid / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003172:Complement C1; D003094:Collagen; D003012:Microbial Collagenase