The catalytic mechanism of glutamyl-tRNA synthetase of Escherichia coli. Evidence for a two-step aminoacylation pathway, and study of the reactivity of the intermediate complex.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D002384:Catalysis; D011756:Diphosphates / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D005975:Glutamate-tRNA Ligase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D008956:Models, Chemical; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism
D011756:Diphosphates; D006898:Hydroxylamines; D012346:RNA, Transfer, Amino Acyl; D019811:Hydroxylamine; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D000604:Amino Acyl-tRNA Synthetases; D005975:Glutamate-tRNA Ligase