title: Selective activation of rabbit ovarian protein kinase isozymes in rabbit ovarian follicles and corpora lutea.
abstract: The magnitude of activation of the type I and type II forms of cAMP-dependent protein kinase was investigated in estrous follicles and corpora lutea (CL) obtained from ovaries of control rabbits and rabbits injected acutely with human chorionic gonadotropin (hCG). To this end, a chromatographic technique which permitted quantitative evaluation of the in vivo activational state of the two forms of cAmP-dependent protein kinase was developed and verified. Results revealed that in follicles obtained from ovaries of untreated estrous rabbits, 15% of the soluble cAMP-dependent protein kinase, all of which exists as the type II isozyme, is activated. Intravenous administration of a single bolus of hCG promoted a concentration-dependent activation (in 10 min) of this protein kinase isozyme. In CL obtained from ovaries of control, 4-day pseudopregnant rabbits, 32% of the total soluble cAMP-dependent protein kinase exists as the type I form and 68% exists as the type II form. Both types of protein kinase are approximately 10% dissociated in CL from ovaries of untreated rabbits. Upon intravenous administration of hCG, only the type I form of cAMP-dependent protein kinase is further activated (in 10 min). Dissociation of this protein kinase is dependent upon the time and concentration of hCG. Preferential activation of the type I form of cAMP-dependent protein kinase in CL is also demonstrable in in vitro studies using exogenous cAMP. These data suggest that the physiological intracellular mediator of acute cAMP-regulated, hCG-triggered functions in rabbit ovarian follicles is the type II isozyme of cAMP-dependent protein kinase while in CL of 4-day pseudopregnant rabbits, it is the type I enzyme form.
mesh_terms: D000818:Animals; D001665:Binding Sites; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000201:enzymology*; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation; D004971:Estrus; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology; D010053:Ovary / Q000201:enzymology*; D011247:Pregnancy; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D011555:Pseudopregnancy / Q000201:enzymology; D011817:Rabbits; D051381:Rats
chemical_list: D006063:Chorionic Gonadotropin; D007527:Isoenzymes; D000242:Cyclic AMP; D011494:Protein Kinases