Limited autolysis of Ca2+-activated neutral protease (CANP) changes its sensitivity to Ca2+ ions.
Ca2+-activated neutral protease (CANP) usually requires mM Ca2+ for activation. The sensitivity of CANP to Ca2+ is greatly enhanced by passing it through a casein-Sepharose column in the presence of Ca2+ ions. This conversion is ascribed to autolysis of CANP. The converted enzyme required 40 microM Ca2+ for 50% activation. Various properties of the converted enzyme were very similar to those of CANP-I, recently found in canine heart muscle. Names of "m-CANP" and "mu-CANP" are proposed for CANPs which require mM and microM order Ca2+ for inactivation, respectively.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002154:Calpain; D002364:Caseins; D002645:Chickens; D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D012685:Sepharose
D002364:Caseins; D012685:Sepharose; D010450:Endopeptidases; D002154:Calpain; D002118:Calcium