Purification of bovine papilloma virus by gel filtration on Sephacryl S-1000 superfine.
Bovine papilloma virus was purified from crude extracts of bovine warts by gel filtration in Sephacryl S-1000 Superfine. Analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis and electron microscopy shows that the virus preparation is as pure as that obtained by alternative techniques. The yield of virus particles is in the range 55-80%. The main advantage of using the gel filtration technique is that it is much less time-consuming than currently used centrifugation procedures.
D000180:Acrylic Resins; D000818:Animals; D052688:Bovine papillomavirus 1 / Q000302:isolation & purification*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D027383:Papillomaviridae / Q000302:isolation & purification*; D014764:Viral Proteins; D014771:Virion / Q000648:ultrastructure
D000180:Acrylic Resins; C037239:Sephacryl Superfine; D014764:Viral Proteins