Odorous chemical perturbations of (Na+ + K+)-dependent ATPase activities. Effects on native and lipid-substituted preparations from individual turbinals from dog olfactory tissue.
Individual turbinals from the right and left sides of dog olfactory tissue were removed and nerve-ending-particle preparations were prepared. (Na+ + K+)-dependent ATPase activities of the individual preparations, and the effect of several odorous compounds [including (+)- and (-)-carvone] on the (Na+ + K+)-dependent ATPase activities, were determined. The maximally stimulatory odorant concentration in the reaction mixture for the majority of odorants was found to be 1.0 mM. Matched pairs of left/right turbinals showed a lack of bilateral symmetry of response. (Na+ + K+)-dependent ATPase activities of various dog brain nerve-ending particle preparations responded only slightly to 1.0 mM odorants. The role of phospholipids in the (Na+ + K+)-dependent ATPase activity was found to be critical. Partial replacement of endogenous lipid with either synthetic phospholipids or extracted lipids resulted in changes in stimulation obtained with endogenous lipids alone.
D020003:1-Octanol; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D000081005:Cyclohexane Monoterpenes; D004285:Dogs; D066298:In Vitro Techniques; D007659:Ketones / Q000494:pharmacology*; D050356:Lipid Metabolism; D039821:Monoterpenes; D000442:Octanols / Q000494:pharmacology; D009812:Odorants; D010743:Phospholipids / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013237:Stereoisomerism; D013729:Terpenes / Q000494:pharmacology*; D014420:Turbinates / Q000187:drug effects / Q000201:enzymology*
D000081005:Cyclohexane Monoterpenes; D007659:Ketones; D039821:Monoterpenes; D000442:Octanols; D010743:Phospholipids; D013729:Terpenes; C006923:carvone; D000254:Sodium-Potassium-Exchanging ATPase; D020003:1-Octanol; C026636:2-nonanone