Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors.
A human gastric adenocarcinoma cell line, HGT-1, was established in vitro from the primary tumor of a 60-year-old patient. Histological examination of the tumor revealed a poorly differentiated adenocarcinoma. Primary tumor cells were cloned in soft agarose and gave rise to tumor colonies. The procedures enabling us to form a continuous cell line from the agarose colonies are described. The cultured cells grew as monolayers of closely apposed polygonal cells with a population-doubling time of 19.48 +/- 1.20 (S.E.) hr during exponential growth at passage 59. They had an epithelial morphology. Ultrastructural studies revealed the presence of microvilli and tight junctions. The HGT-1 cell line is tumorigenic in nude mice and has a hyperdiploid karyotype with a modal number of 57 chromosomes. It exhibits numerous marker chromosomes. These human gastric epithelial cells do not secrete mucus or carcinoembryonic antigen. They exhibit functional histamine H2-receptors mediating cellular cyclic adenosine 3':5'-monophosphate production and adenylate cyclase activation. In conclusion, the use of a soft-agarose clonogenic assay permitted us to develop a cancer cell line without the problems of fibroblastic cell contamination. The existence of histamine H2-receptors on gastric HGT-1 cells stresses the importance of this line as a model for studies of regulatory mechanisms involved in gastric secretion.
D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002875:Chromosomes; D000242:Cyclic AMP / Q000096:biosynthesis; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011968:Receptors, Histamine / Q000032:analysis*; D011970:Receptors, Histamine H2 / Q000032:analysis*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
D011968:Receptors, Histamine; D011970:Receptors, Histamine H2; D000242:Cyclic AMP; D000262:Adenylyl Cyclases