title: A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.
abstract: Gene III protein of bacteriophage f1 is inserted into the host cell membrane where it is assembled into phage particles. A truncated form of gene III protein, encoded by a recombinant plasmid and lacking the carboxyl terminus, does not remain in the membrane but instead appears to slip through it. Fusion of a hydrophobic "membrane anchor" from another membrane protein, the gene VIII protein, to the truncated gene III protein (by manipulation of the recombinant plasmid) restores membrane anchoring. A model for the relationship of gene III protein with the Escherichia coli membrane is discussed.
mesh_terms: D002462:Cell Membrane / Q000502:physiology; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D004262:DNA Restriction Enzymes