The development of rat dorsal root ganglia in three experimental media containing Landry-Guillain-Barré, alcoholic peripheral neuropathy or normal adult sera.
Development of rat dorsal root ganglia in vitro mimics the development of rat dorsal root ganglia in vivo when grown in medium containing human cord serum. When serum from patients either in the acute phase of Landry-Guillain-Barré syndrome or with alcoholic peripheral neuropathy is substituted for human cord serum, maturation is inhibited. Development of fetal relationships occurs between the Schwann cells and axons and many axons become enclosed within the processes of a single Schwann cell. Formation of myelin does not occur. The substitution of normal adult blood for cord serum also inhibits maturation, but unlike development with neuropathic serum, the Schwann cells and axons do not become associated and myelination does not occur.
D000437:Alcoholism / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D000818:Animals; D003470:Culture Media; D005312:Fetal Blood / Q000032:analysis; D005727:Ganglia, Spinal / Q000254:growth & development*; D006801:Humans; D009924:Organ Culture Techniques; D010523:Peripheral Nervous System Diseases / Q000097:blood / Q000209:etiology / Q000503:physiopathology*; D011129:Polyradiculoneuropathy / Q000097:blood / Q000503:physiopathology*; D051381:Rats; D012583:Schwann Cells / Q000502:physiology
D003470:Culture Media