Antibodies against a synthetic peptide of the poliovirus replicase protein: reaction with native, virus-encoded proteins and inhibition of virus-specific polymerase activities in vitro.
A carboxy-terminal peptide of the poliovirus replicase protein (p63) was chemically synthesized, coupled to bovine serum albumin carrier, and injected into rabbits. The resulting antisera reacted with six virus-specific proteins from HeLa cells infected with poliovirus: NCVP 0b, NCVP 1b, NCVP 2, a protein of about 60,000 daltons, p63, and NCVP 6b. The identity of the 60,000-dalton protein is not known, but the other results were consistent with previous experimental approaches which demonstrated that p63 and the other four polypeptides have common coding sequences. An amino-terminal peptide of p63 failed to elicit an immune response in rabbits. Antibodies raised against the p63 carboxy-terminal peptide inhibited poliovirus replicase and polyuridylic acid polymerase activities in vitro, providing strong support for earlier suggestions that these activities are a property of a single virus-specific polypeptide.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D006801:Humans; D009713:Nucleotidyltransferases / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D017955:Poliovirus / Q000201:enzymology* / Q000276:immunology; D012316:RNA Nucleotidyltransferases / Q000276:immunology*; D012324:RNA-Dependent RNA Polymerase / Q000276:immunology*; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D010455:Peptides; D014764:Viral Proteins; D009713:Nucleotidyltransferases; D012316:RNA Nucleotidyltransferases; C022428:poly U polymerase; D012324:RNA-Dependent RNA Polymerase