Induction and abrogation of suppressor cell function in humans: effect on B cell activation by different polyclonal activators.
In a reverse plaque forming cell (PFC) assay, the effect of concanavalin A (Con-A)-induced suppressor cells on polyclonally activated B lymphocytes was studied. Differentiation to PFC, as induced by pokeweed mitogen (PWM), Staphylococcus aureus or Epstein-Barr virus (EBV) was in all cases suppressed by the addition of ConA-pretreated cells. The EBV-stimulated cells showed the smallest PFC response and appeared least amenable to suppression. IgA-secreting cells were less suppressible than IgM- and IgG-secreting cells regardless of the polyclonal B lymphocyte activator used. The suppressor cells were radiosensitive.
D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000528:radiation effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013205:Staphylococcal Protein A / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D011043:Pokeweed Mitogens; D013205:Staphylococcal Protein A; D003208:Concanavalin A