The role of superoxide and hydroxyl radicals in phospholipid peroxidation catalysed by iron salts.
Iron(II) salts in aqueous solution, or iron(III) salts in the presence of an O.-2 generating system, can activate dioxygen to produce hydroxyl radicals. These are detected indirectly by their ability to degrade deoxyribose with the formation of thiobarbituric acid-reactive (TBA) products. Iron salts also catalyse the peroxidation of phospholipids resulting in the formation of TBA-reactive products. Hydroxyl radicals were responsible for the degradation of deoxyribose but not for the observed peroxidation of phospholipid. The function of O.-2 in both deoxyribose degradation and phospholipid peroxidation seems to be that of reducing iron(III) into iron(II).
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D006878:Hydroxides; D017665:Hydroxyl Radical; D007501:Iron; D008054:Lipid Peroxides; D008099:Liver / Q000201:enzymology; D010100:Oxygen; D010743:Phospholipids; D013482:Superoxide Dismutase / Q000097:blood; D013481:Superoxides; D014969:Xanthine Oxidase / Q000378:metabolism
D006878:Hydroxides; D008054:Lipid Peroxides; D010743:Phospholipids; D013481:Superoxides; D017665:Hydroxyl Radical; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D010100:Oxygen