A monoisozymic nucleoside diphosphate kinase capable of complete phosphorylation.
Nucleoside diphosphate kinase (EC 2.7.4.6) has been purified nearly 2000-fold from beef brain particulate material in 65% yield. The purification procedure described here takes advantage of the relatively unique complexation of the enzyme with blue dextran-Sepharose in the presence of a nonionic detergent. Unlike preparations of the enzyme previously described, the bovine brain enzyme is monoisozymic and each polypeptide chain can be phosphorylated by a nucleoside triphosphate substrate. Catalytically functional forms of the enzyme having lower pI values can be generated by proteolysis.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D007700:Kinetics; D008970:Molecular Weight; D009701:Nucleoside-Diphosphate Kinase / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D010770:Phosphotransferases; D009701:Nucleoside-Diphosphate Kinase