title: Significance of prednisolone administration for hepatic mitochondrial function of the rat with biliary obstruction.
abstract: Mitochondrial respiratory function of the liver is disturbed in biliary obstruction, especially in that caused by tumours in the hepatobiliary system. This study aimed to clarify whether a glucocorticoid, prednisolone succinate, is effective to improve the reduced mitochondrial function of the rat liver in obstructive jaundice. Five doses of 5 mg/kg or 25 mg/kg prednisolone succinate were administered at 5 consecutive days to the rats after 1, 3 or 6 weeks of biliary obstruction and to the rats without obstruction, and the hepatic mitochondrial function and contents of cytochromes of these rats were investigated. With 25 mg/kg prednisolone, hepatic mitochondrial function was improved in rats with biliary obstruction of 3- or 6-weeks duration as compared with the prednisolone-untreated group. Compensatory increase or recuperation of the once decreased cytochrome A(+a3) and/or turnover number of phosphorylation was also observed in 25 mg/kg group. Hepatic mitochondrial function of obstruction-free animals was rather impaired by treatment with 25 mg/kg prednisolone. In 5 mg/kg group, above mentioned effects were scarcely observed. It was concluded that the hepatic mitochondrial function in animals with prolonged biliary obstruction, as same as in animals with short-term obstruction, can be improved by the administration of adequately large amounts of prednisolone.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002779:Cholestasis / Q000188:drug therapy* / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D003575:Cytochromes c1 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D003576:Electron Transport Complex IV / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption; D010766:Phosphorylation; D011239:Prednisolone / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003574:Cytochrome c Group; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003575:Cytochromes c1; D011239:Prednisolone; D003576:Electron Transport Complex IV