Expression of Drosophila heat shock genes is regulated in Rat-cells.
We have introduced a Drosophila 87A heat shock locus into the genome of Rat-1 cells. A brief heat shock of a resultant transformed line, Rhs11, induces the transcription of the Drosophila heat shock genes from the Drosophila heat shock promoter. This suggests that the induction mechanism and regulatory sequences for the heat shock response have been conserved between Drosophila and mammals.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004330:Drosophila / Q000235:genetics*; D005796:Genes; D006360:Heat-Shock Proteins; D006358:Hot Temperature; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D006360:Heat-Shock Proteins; D011506:Proteins; D004262:DNA Restriction Enzymes