Effect of sodium butyrate on the stimulation of casein gene expression by prolactin.
Sodium butyrate, but not isobutyrate, inhibits prolactin action on the induction of casein synthesis and casein mRNA accumulation in rabbit mammary explants. Sodium butyrate specifically prevents the generation of the prolactin relay which can be released from isolated membranes incubated with prolactin and which stimulates directly casein gene transcription when added to isolated mammary nuclei. This indicates that sodium butyrate exerts its inhibitory action essentially at the membrane level.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002364:Caseins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000187:drug effects; D011388:Prolactin / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000187:drug effects; D011981:Receptors, Prolactin
D002087:Butyrates; D002364:Caseins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D020148:Butyric Acid; D011388:Prolactin