Molecular cloning and chromosomal mapping of a human locus related to the transforming gene of Moloney murine sarcoma virus.
Human DNA was analyzed for the presence of sequences homologous to the transforming gene (v-mos) of Moloney murine sarcoma virus. A single 2.5-kilobase pair (kbp) EcoRI-generated fragment of human DNA was identified by using cloned v-mos as probe. This DNA was molecularly cloned in a bacteriophage vector. By heteroduplex and restriction enzyme analyses, this human DNA fragment, designated c-mos (human), contained a 0.65-kbp region of continuous homology with v-mos and was present as a single copy in human DNA. By testing for the presence of c-mos (human) in somatic cell hybrids possessing various numbers of human chromosomes, as well as in subclones of such hybrids, it was possible to assign c-mos (human) to human chromosome 8.
D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000502:physiology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005260:Female; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010920:Placenta; D011247:Pregnancy
D004262:DNA Restriction Enzymes