Copy number mutations (Cop-) of the plasmid containing the replication origin (oriC) of the Escherichia coli chromosome: lethal effect of the cop region cloned onto a high-copy-number vector on host cells.
High-copy-number mutants were isolated from an oriC plasmid. They carried insertion mutations within a region (about 470 base pairs) near the uncB gene. When a segment containing this region was cloned onto a high-copy-number plasmid, such a plasmid could be maintained as an intact form only when it was present in a lower copy number.
D002876:Chromosomes, Bacterial / Q000378:metabolism; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids
D004262:DNA Restriction Enzymes