title: Is Percoll innocuous to cells?
abstract: Peritoneal macrophages from mice, isolated rat liver Kupffer cells and rat testis Leydig cells ingested large numbers of Percoll particles, a gradient medium widely used for separation of cells and subcellular organelles by density-gradient centrifugation. A decrease in the percentage of macrophages adhering to plastic also occurred after exposure of the cells to Percoll, even at 4 degrees C, a temperature at which Percoll was not ingested. The effect of Percoll on macrophage adherence may involve a loose association between the density medium and the cell surface. Other cell-surface-related phenomena may also be affected by prior exposure of cells to Percoll.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D003102:Colloids / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007728:Kupffer Cells / Q000187:drug effects; D007985:Leydig Cells / Q000187:drug effects; D008099:Liver / Q000166:cytology; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D011205:Povidone / Q000494:pharmacology*; D051381:Rats; D012822:Silicon Dioxide / Q000494:pharmacology*
chemical_list: D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone