title: The inactivation of the bovine J blood group substance by the periodate ion.
abstract: 1. Treatment of J-positive (Jcs) bovine erythrocytes with periodate (0.25 mmol/l final concentration, 1 hour, room temperature) has no effect on the J activity. Higher periodate concentrations cause spontaneous haemolyses. 2. Treatment of the lipids extracted from (and containing all J activity of) Jcs erythrocytes with periodate leads to a decrease of J activity even with lower periodate concentrations. 3. Treatment of the stroma prepared from Jcs erythrocytes with periodate demonstrated the relative stability of the J antigen up to 0.25 mmol/l periodate. At the same time the sialic acid concentration of stroma is reduced to about 13% of the initial concentration. 4. Desialylation of Jcs erythrocytes or Jcs stroma with sialidase does not affect the J activity thus confirming previous findings. On the other hand, the J activity of desialylated Jcs stroma is much more susceptible to periodate. 5. It is concluded that membrane-bound sialic acid shields the membrane-bound J antigen from being attacked by periodate.
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000378:metabolism*; D002417:Cattle; D003864:Depression, Chemical; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000097:blood; D009439:Neuraminidase / Q000494:pharmacology; D010101:Oxygen Consumption; D010504:Periodic Acid / Q000494:pharmacology*
chemical_list: D001789:Blood Group Antigens; D008055:Lipids; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase