title: Prostacyclin binding to guinea pig pulmonary receptors.
abstract: [3H] Prostacyclin bound to membranes of guinea pig lung. Specific binding was saturable, and the results revealed two receptor classes (Kd =16 nM and 258 nM). The binding capacity of the high affinity site was 105 fmol [3H]prostacyclin per mg of membrane protein, and that of the low affinity site was 1257 fmol per mg membrane protein. A comparison of selected prostaglandins as inhibitors of [3H] prostacyclin binding revealed some of the structural requriements of the ligand for occupation of the high affinity receptor.
mesh_terms: D000818:Animals; D011464:Epoprostenol / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*; D011465:Prostaglandins, Synthetic / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011453:Prostaglandins; D011465:Prostaglandins, Synthetic; D011956:Receptors, Cell Surface; D011982:Receptors, Prostaglandin; D011464:Epoprostenol