Solvent drag effect in drug intestinal absorption. I. Studies on drug and D2O absorption clearances.
It was shown that the intestinal absorption clearance of D2O (CLD2O) could be a more appropriate index to study the solvent drag effect than water volume flow which was the difference between water influx and outflux in the intestinal lumen. Then, the correlation between the intestinal absorption clearances of drugs (CLdrug) and CLD2O were studied using the in situ recirculating method in the rat small intestine. The drugs used were low molecular drugs, that is, benzoic acid, salicylic acid, p-hydroxybenzoic acid and antipyrine, and comparably high molecular drugs, that is, cephalexin (CEX), cefroxadine (CXD) and cephalothin (CET). CLdrug and CLD2O were obtained in hypertonic, isotonic and hypotonic perfused solution adjusted with sodium chloride. Consequently, the correlations for all drugs except CET were significant and high solvent drag effects were observed. CLdrug of benzoic acid, salicylic acid and antipyrine were approximately equal to CLD2O, suggesting that the intestinal mucosa could not distinguish these lower molecular drugs from water. For the high molecular drugs such as cephalosporins, however, some extent of reflection from the membrane was certainly found in CEX and CXD, and the extent in CET was assumed much larger than CEX and CXD, resulting that the contribution of solvent drag in CET could not be found. Consequently, it was suggested that the solvent drag had some important role in the intestinal absorption of cephalosporins.
D000818:Animals; D000983:Antipyrine / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D002511:Cephalosporins / Q000378:metabolism; D003903:Deuterium / Q000378:metabolism*; D017666:Deuterium Oxide; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D009997:Osmotic Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D012997:Solvents / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
D001565:Benzoates; D002511:Cephalosporins; D012459:Salicylates; D012997:Solvents; D014867:Water; D003903:Deuterium; D017666:Deuterium Oxide; D020156:Salicylic Acid; D000983:Antipyrine