title: Microinjection of nucleic acids into cultured mammalian cells by electrophoresis.
abstract: Simian virus 40 (SV40) DNA was microinjected into cultured mammalian cells by means of electrophoresis (iontophoresis). Successful transfer of DNA into cells was confirmed by detecting SV40 T antigen using the indirect immunofluorescent technique.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics*; D004586:Electrophoresis; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology / Q000276:immunology; D008845:Microinjections / Q000379:methods; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral