Frequencies of specific antibody-producing cells for sheep red blood cells and for phosphorylcholine in human tonsillar B lymphocytes transformed by Epstein-Barr virus.
The frequencies of transformed B cells and of transformed specific antibody-producing cells in human tonsillar lymphocytes infected with Epstein-Barr virus (EBV) were determined by limiting dilution analyses. The latter was corrected by the former and the frequencies of specific antibody-producing cells in EBV-transformed B cell populations were obtained. The frequencies of specific B cells for sheep red blood cells were calculated to be one in 500 to 2,000 EBV-transformed tonsillar B cells obtained from four donors, while those of specific B cells for a hapten, phosphorylcholine, were one in 700 to 5,000. The method may be useful to define the repertoire of specificities in the human B cell populations.
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D002452:Cell Count; D002472:Cell Transformation, Viral; D004912:Erythrocytes / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D014066:Palatine Tonsil / Q000166:cytology; D010767:Phosphorylcholine / Q000276:immunology
D010767:Phosphorylcholine