Exogenous DNA transcription in cells with their native DNA inhibited. 4. Demonstration of specific antigens codified by exogenous DNA.
It is possible to obtain antisera to cancer-specific antigens of mouse Ehrlich ascites cancer (EAC) cells when chimera rabbits previously made unresponsive to immunological stimulation by normal mouse cells antigens are immunized with mouse cancer cells. Employing this specifically anti-cancerous immunoserum this work shows that EAC cells and TC-SV40 cells contain cross-reacting cancer-specific antigens. Exogenous DNA from EAC cells is demonstrated as being able to display its coded information when it is incorporated to endogenous TC-SV40 cell DNA whose activity has been previously inhibited by 5'-bromodeoxyuridine.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D003429:Cross Reactions; D004273:DNA, Neoplasm / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D051379:Mice; D011817:Rabbits; D013539:Simian virus 40 / Q000276:immunology; D014158:Transcription, Genetic
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine