[Dibromoethylacetate - a new agent for fixing the unwound regions of DNA. Denaturation maps of mitochondrial DNA from the rat liver].
On the basis of the reaction of dibromoethylacetate with adenine and cytosine at the uncoiled regions of DNA it was possible to fix these molten regions (the degree of denaturation was about 16%) for rat liver mitochondrial DNA. Modification of all accessible adenine and cytosine residues in 0.14 M Na-acetate, pH 5.65, was completed at 70-80 degrees C in 15-20 min. Using the computer orientation for the set of fixed BamHI-fragments and the linear molecules of full length the denaturation map of mtDNA was constructed, the GC-content of the molten regions was about 28%. The dibromoethylacetate is a perspective agent for screening DNA with a low content of destabilizing regions (for instance, DNA of malignant cells).
D000225:Adenine; D000818:Animals; D003596:Cytosine; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015946:Ethylene Dibromide / Q000494:pharmacology* / Q000031:analogs & derivatives; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D051381:Rats
D004272:DNA, Mitochondrial; D006842:Hydrocarbons, Brominated; D015946:Ethylene Dibromide; D003596:Cytosine; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D000225:Adenine; C018508:1,2-dibromoethyl acetate
Dibrométilatsetat - novyĭ agent dlia fiksatsii despiralizovannykh uchastkov DNK. Denaturatsionnaia karta mitokhondrial'noĭ DNK pecheni krysy.