title: Long-term preservation of transfecting activity of avian sarcoma proviral DNA.
abstract: The integrated proviral DNA of avian sarcoma virus (ASV) in host cell chromosomes has been isolated and stored in saline sodium citrate (SSC) solution or in 70% ethanol at 4 degrees C in a refrigerator over 4 years. This DNA was assayed by transfection of chick embryo cells(CEC). The biological activity of cellular transformation by the stored DNA was compared with that of a fresh isolate of the proviral DNA. The efficiency of the transfection by each DNA was almost the same.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D051379:Mice; D011309:Preservation, Biological; D051381:Rats; D012034:Refrigeration; D013997:Time Factors; D014162:Transfection
chemical_list: D004279:DNA, Viral