title: A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P.
abstract: A novel mutation in the anticodon stem of E. coli tRNA1Tyrsu3+ (G43 to U43) has been characterized. The gene coding for the mutant tRNA, carried by phage phi 80DHA61.3 a derivative of phi 80psu3+su0, produces only 20% of mature suppressor tRNA as compared with phi 80psu3+. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRNA coded for by phi 80DHA61.3 is processed by RNase P more slowly than the su3+ precursor and does not form as stable an enzyme-substrate complex as does su3+ precursor. phi 80 DHA61.3 also contains a large deletion which begins in the spacer region between the su3+ gene and the su0- gene, extends through the su0- gene and includes most of the repeated region following the tRNA genes.
mesh_terms: D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006147:Guanine; D007700:Kinetics; D009154:Mutation; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D043262:Ribonuclease P; D014498:Uracil
chemical_list: D000926:Anticodon; D029968:Escherichia coli Proteins; D012346:RNA, Transfer, Amino Acyl; D014498:Uracil; D006147:Guanine; D004722:Endoribonucleases; D004262:DNA Restriction Enzymes; D043262:Ribonuclease P; C470865:ribonuclease P, E coli