Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans.
A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicated in both organisms, had unique sites for two restriction endonucleases, conferred ampicillin resistance on both organisms, and could be used as a cloning vector in A. nidulans.
D000667:Ampicillin / Q000494:pharmacology; D000458:Cyanobacteria / Q000235:genetics*; D004261:DNA Replication; D004251:DNA Transposable Elements; D004274:DNA, Recombinant / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D010403:Penicillin Resistance; D010957:Plasmids; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D000667:Ampicillin