title: Molecular cloning of AKR xenotropic murine leukemia virus unintegrated proviral DNA.
abstract: Suprahelical proviral DNA of AKR xenotropic murine leukemia virus was purified from agarose gels and cloned in lambda Charon 28 DNA (BamHI sites). Nine viral DNA recombinants were identified and mapped with 12 restriction endonucleases. Three calsses of cloned viral DNA inserts were found: (1) Six inserts were apparently full-length 9.0-kb DNA with tandem long terminal repeat (LTR) elements; (2) two inserts contained DNAs with deletions in or adjacent to the LTR regions; (3) a single isolate contained an inversion of 2.3 kb around the LTR in the envelope gene.
mesh_terms: D000406:AKR murine leukemia virus / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant / Q000032:analysis; D004279:DNA, Viral / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes