title: Influence of cellular and paracellular conductance patterns on epithelial transport and metabolism.
abstract: Theoretical analysis of transepithelial active Na transport is often based on equivalent electrical circuits comprising discrete parallel active and passive pathways. Recent findings show, however, that Na+ pumps are distributed over the entire basal lateral surface of epithelial cells. This suggests that Na+ that has been actively transported into paracellular channels may to some extent return to the apical (mucosal) bathing solution, depending on the relative conductances of the pathways via the tight junctions and the lateral intercellular spaces. Such circulation, as well as the relative conductance of cellular and paracellular pathways, may have an important influence on the relationships between parameters of transcellular and transepithelial active transport and metabolism. These relationships were examined by equivalent circuit analysis of active Na transport, Na conductance, the electromotive force of Na transport, the "stoichiometry" of transport, and the degree of coupling of transport to metabolism. Although the model is too crude to permit precise quantification, important qualitative differences are predicted between "loose" and "tight" epithelia in the absence and presence of circulation. In contrast, there is no effect on the free energy of metabolic reaction estimated from a linear thermodynamic formalism. Also of interest are implications concerning the experimental evaluation of passive paracellular conductance following abolition of active transport, and the use of the cellular voltage-divider ratio to estimate the relative conductances of apical and basal lateral plasma membranes.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D004553:Electric Conductivity; D004848:Epithelium / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D008433:Mathematics; D008564:Membrane Potentials; D008954:Models, Biological; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D007473:Ion Channels; D012965:Sodium Chloride; D012964:Sodium