title: Influence of coenzyme A on the in vitro binding of benzo(a)pyrene metabolites to protein.
abstract: Incubation of 20,000 xg supernatants of liver homogenates from female Wistar rats with benzo(a)pyrene (BP) and other cofactors in the presence of coenzyme A (CoA) produced metabolites that required extensive hydrolysis successively in 2N NaOH, 2N HCl, 2N NaOH and 6N HCl for their release. It is suggested that coenzyme A activated benzpyrenyl-amino acid formed via epoxide(s). The activated amino acid(s) became bound to thiol protein.
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D003065:Coenzyme A / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D013058:Mass Spectrometry; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001580:Benzopyrenes; D001564:Benzo(a)pyrene; D003065:Coenzyme A