[Isolation of mutants with an increased frequency of transposon Tn1 translocations in Escherichia coli K-12 cells].
Two het mutations (high efficiency of transposition) were isolated and characterized preliminarily. The mutations lead to increasing the frequency of ampicillin transposon Tn1 translocation into different replicons in Escherichia coli cells. Both mutations increase the frequency of transposition by the same degree. One of het mutations has been localized within Tn1 transposition element. This mutation seems to be similar to mutations isolated earlier (Gill et al., 1978) in the transposon Tn3 repressor gene which occurred at a high frequency and led to an increase in transposition. The other het mutation is situated in the bacterial chromosome.
D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D012093:Replicon; D013489:Suppression, Genetic; D014178:Translocation, Genetic
D004251:DNA Transposable Elements
Vydelenie mutantov s povyshennoĭ chastotoĭ translokatsii transpozona Tn1 v kletkakh Escherichia coli K-12.