The application of a sepharose bead immunofluorescence assay and a solid-phase radioimmunoassay to the bovine leukemia virus system.
Several fluorescence assays with bovine leukemia virus (BLV) conjugated to activated Sepharose 4B were used for the detection of BLV and anti-BLV antibodies. These tests were compared with a solid-phase radioimmunoassay and found to be in the same sensitivity range. Sepharose bead immunofluorescence assay and solid-phase radioimmunoassay can be applied to the diagnosis of BLV infection in cattle.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*; D012190:Retroviridae / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral