Construction of a hybrid plasmid molecule containing the total coding region of Semliki Forest virus 26S mRNA.
We have constructed a hybrid plasmid molecule that contains the complete coding sequences from the 26S mRNA of Semliki Forest virus. Five fragments which together covered the mRNA sequence were isolated from three original hybrid plasmids and joined together at five different restriction endonuclease cleavage sites using T4 DNA ligase.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005814:Genes, Viral; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012672:Semliki forest virus / Q000235:genetics*
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012367:RNA, Viral; D004262:DNA Restriction Enzymes