Development of a radioimmunoassay for an amino-terminal peptide of pro-opiocortin containing the gamma-MSH region: measurement and characterization in human plasma.
A radioimmunoassay has been developed for the N-terminal region of human pituitary pro-opiocortin (N-POC), the common precursor protein of ACTH and beta-LPH, using an antiserum which recognizes residues near the gamma-MSH region. The concentrations of greater than 300 ng/l of immunoreactive peptide were determined in unextracted human plasma, the relative molecular mass of the reacting fragments corresponding to a seventy-seven amino acid glycoprotein. The concentrations of immunoreactive N-POC peptides were correlated with those of ACTH in plasma obtained from patients with various disorders of the hypothalamic-pituitary-adrenal axis.
D000182:ACTH Syndrome, Ectopic / Q000097:blood; D000224:Addison Disease / Q000097:blood; D000324:Adrenocorticotropic Hormone / Q000097:blood*; D003480:Cushing Syndrome / Q000097:blood; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000097:blood*; D009347:Nelson Syndrome / Q000097:blood; D010908:Pituitary Hormones, Anterior / Q000097:blood*; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods
D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones