[Creation of an avirulent mutant of Aujeszky's disease virus by exposure to 5-bromodeoxyuridine].
A mutant with new biologic properties has been obtained at the cultivation of a virulent strain of the Aujeszky's disease virus in chick embryo fibroblast cultures, following a definite number of passages. It has proved resistant when culturing at the present of 5-brom-desoxyuridine and 5-iod-2-desoxyuridine, retaining its infections titer. It produces small plaques and destroys cell cultures of a tiny granular structure, the plaque-forming titer being 5. 6X10-7. The mutant has been shown to be avirulent for sheep, suckling pigs, and rabbits. In confers solid and lasting immunity in vaccinated animals which are virus-free and show no virus excretion. The newly obtained MK-35 mutant has proved to be stable in terms of its new biologic properties.
D000818:Animals; D000833:Animals, Suckling; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002642:Chick Embryo; D011558:Herpesvirus 1, Suid / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity; D007109:Immunity; D051379:Mice; D009154:Mutation; D011817:Rabbits; D012756:Sheep; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology / Q000302:isolation & purification; D014774:Virulence / Q000187:drug effects
D014765:Viral Vaccines; D001973:Bromodeoxyuridine
Sŭzdavane na avirulenten mutant ot virusa na Aueski pod vŭzdeĭstvieto na 5-bromdoezoksiuridin.