Identification of N- and C-terminal corticotropin peptides in the Mr 80000 form of neurophysin.
The 125I-labeled Mr 80000 form of neurophysin has been purified from bovine neurohypophysi. Tryptic digests of this species were analyzed, prior to or after treatment with carboxypeptidase B, by high-pressure liquid chromatography followed by isoelectric focusing and the fragments compared with those generated by a similar treatment of reference bovine 1-39 adrenocorticotropin. The ACTH peptides 22-39 and 1-8, as well as the 1-7 derivative of the latter were identified by those two independent criteria. This provides chemical evidence supporting the hypothesis [8] that high Mr neurophysin may contain the sequence of ACTH.
D000324:Adrenocorticotropic Hormone; D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D046911:Macromolecular Substances; D008970:Molecular Weight; D009481:Neurophysins / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis*; D014357:Trypsin
D046911:Macromolecular Substances; D009481:Neurophysins; D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D014357:Trypsin