title: Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene.
abstract: We have isolated a lambda-transducing phage carrying the gene (glnS) for Escherichia coli glutaminyl-tRNA synthetase. The location of the glnS gene within the 13.5-kilobase E. coli DNA transducing fragment was determined by genetic means. The glnS gene was recloned into plasmid pBR322 and its nucleotide sequence was established. The DNA sequence translates to a protein of 550 amino acids.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005796:Genes; D009154:Mutation; D010957:Plasmids; D014161:Transduction, Genetic
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase