title: Changes in DNA binding by purified simian RNA polymerase II under transcribing and nontranscribing conditions.
abstract: The interaction of RNA polymerase II from African Green Monkey liver tissue with SV40 DNA was examined by a DNAase protection technique. In the absence of nucleoside triphosphates, simian polymerase binds to nicked, linear SV40 DNA and protects 30 bp of binary complex DNA from DNAase I digestion. With the addition of nucleoside triphosphates to initiate transcription, polymerase protects 40 bp of the ternary complex DNA from DNAase I. Thus, a conformational change in either the polymerase, the DNA, or both occurs during the transition from binary to ternary complex, and this altered conformation allows a larger protection of template DNA. Similar results were seen with Escherichia coli RNA polymerase holoenzyme on SV40 DNA.
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases