title: An estrogen-binding protein and endogenous ligand in Saccharomyces cerevisiae: possible hormone receptor system.
abstract: A protein macromolecule in the cytosol of the unicellular eukaryotic yeast Saccharomyces cerevisiae selectively binds the vertebrate estrogen hormone 17 beta-estradiol with high affinity. Lipid extracts of the yeast cells or the conditioned growth medium yield a substance that can bind competitively to the tritiated estradiol-binding sites in the yeast  and to mammalian estrogen receptors. These findings suggest that the binding protein may be a primitive hormone receptor and that the lipid-extractable substance represents the endogenous ligand.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D005260:Female; D008024:Ligands; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface; D011960:Receptors, Estrogen