The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid.
We have found that the transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus all contain tightly bound lipid. This modification could play a role in the binding of these proteins to cellular membranes. The lipid associated with p60src, the transforming protein of Rous sarcoma virus, is located in the NH2-terminal domain of the polypeptide. This is the region of the protein that has been shown previously to participate in binding the protein to membranes. Two mature forms of p21, the transforming protein of Harvey sarcoma virus, contain lipid. Lipid is not, however, associated with newly synthesized p21. While mature p60src and p21 are bound to cellular membranes, the newly synthesized forms of these proteins are not. The posttranslational addition of lipid may therefore be the means by which these proteins acquire an affinity for membranes.
D000011:Abelson murine leukemia virus / Q000235:genetics*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D015689:Oncogene Protein p21(ras); D015688:Oncogene Protein pp60(v-src); D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000302:isolation & purification; D011990:Receptors, Transferrin; D012190:Retroviridae / Q000235:genetics*; D014168:Transferrin / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification*
D010169:Palmitic Acids; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin; D014764:Viral Proteins; D019308:Palmitic Acid; D015688:Oncogene Protein pp60(v-src); D015689:Oncogene Protein p21(ras)