Analysis of viral and defective-interfering nucleocapsids in acute and persistent infection by rhabdoviruses.
We have isolated and characterized the RNA of intracellular virus nucleocapsids recovered from a number of cell cultures persistently infected with rabies virus or vesicular stomatitis virus (VSV). VSV persistent infections in BHK21, L cells and Aedes albopictus (mosquito) cells generally showed the presence of large amounts of defective-interfering (DI) nucleocapsid RNA and much smaller amounts of standard (B) nucleocapsid RNA. Persistent infections of BHK21 cells by two rabies virus strains, challenge virus standard (CVS-11) or HEP-Flury, were followed for several months during which time the ratio of DI to B nucleocapsid RNA cycled dramatically. We also observed coordinated fluctuations in the absolute amount of incorporation of [3H]uridine into virus nucleocapsid RNA. Total incorporation was generally highest following a decrease in the relative amount of DI nucleocapsid RNA synthesis. At no time were DI nucleocapsids absent in any of the persistently infected cultures.
D000330:Aedes; D000818:Animals; D002213:Capsid / Q000032:analysis*; D002460:Cell Line; D003673:Defective Viruses / Q000032:analysis* / Q000254:growth & development; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D011817:Rabbits; D011820:Rabies virus / Q000032:analysis*; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000254:growth & development; D014762:Viral Interference; D014764:Viral Proteins / Q000032:analysis*
D012367:RNA, Viral; D014764:Viral Proteins