GABA abolishes cooperativity between benzodiazepine receptors.
Benzodiazepine receptors on neuronal membranes demonstrate cooperative interactions; these can be studied by observing the rates at which [3H]flunitrazepam dissociates from the receptors at different levels of receptor occupancy. In this study, the effect of GABA on the rates of reaction of [3H]flunitrazepam with benzodiazepine receptors is examined. GABA increases the association rate and decreases the dissociation rate of this ligand. In the presence of GABA, it is no longer possible to observe cooperativity between benzodiazepine receptors.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005445:Flunitrazepam / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam