title: Further studies on mevalonate phosphorylation in neonatal chick brain.
abstract: Mevalonate phosphorylation was studied in neonatal chick brain. Formation of phosphorylated derivatives of mevalonic acid increased with the pH in the range assays (5.5-9.5). Phosphomevalonate kinase was completely inactivated after treatment at 50 degree C for 5-10 min, whereas mevalonate kinase was found to retain its activity under the same conditions. Exposure to 65 degree C for 5 min resulted in the inactivation of mevalonate kinase. Both mevalonate-activating enzymes from chick brain were located primarily in the soluble fraction. The amounts of phosphomevalonic acid and pyrophosphomevalonic acid did not show a significant diurnal variation to suggest the presence of a circadian rhythm in either kinase. Cholesterol feeding and fasting had no effect on mevalonate phosphorylation by neonatal chick brain.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D002645:Chickens; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004355:Drug Stability; D005215:Fasting; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D017856:Phosphotransferases (Phosphate Group Acceptor)
chemical_list: D002791:Cholesterol, Dietary; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase; D017856:Phosphotransferases (Phosphate Group Acceptor); C022371:phosphomevalonate kinase; D008798:Mevalonic Acid