[Restrictase BamHI, HindII, EcoRI and SmaI mapping of hybrid lambda-phi 80 phages with recombination in the structural gene region].
Hybrids lambda H lambda T80 with recombination in the region of structural genes have lambda head and phi 80 tail genes. In this paper the molecular structure of 5 independently isolated hybrids was established using restriction endonucleases. It has been shown that all of them have a recombinant head or tail. A deletion of 4,8% lambda was demonstrated in the immunity region of phi 80vir phage. Co-ordinates of restriction sites for BamHI, HindIII, EcoRI and SmaI restriction endonucleases on phi 80 DNA were calculated.
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics*; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D011995:Recombination, Genetic
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Kartirovanie restriktazami BamHI, HindII, EcoRI i SmaI gibridnykh fagov lambda-fi 80 s rekombinatsieĭ v oblasti strukturnykh genov.