Isolation and immunochemical localization of collagenase in mouse peritoneal macrophages.
Collagenase was isolated from the culture medium of thioglycollate-stimulated mouse peritoneal exudate macrophages. The macrophage collagenase activity was inhibited by goat anti-mouse bone collagenase antibody, indicating that macrophage collagenase immunologically cross-reacts with mouse bone collagenase. The enzyme was localized in mouse peritoneal macrophages by indirect immunofluorescent antibody technique. Distinct granular fluorescence was observed intracellularly in most thioglycollate-stimulated macrophages, whereas slight or no fluorescence was observed in non-stimulated control macrophages.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001842:Bone and Bones / Q000201:enzymology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D003012:Microbial Collagenase / Q000302:isolation & purification*
D003012:Microbial Collagenase