Mapping the alpha-globin genes in Hb J Mexico carriers.
The organization of the alpha-globin genes was studied by restriction endonuclease mapping, in subjects carrying the alpha variant Hb J Mexico. A subject homozygous for Hb J synthesized both Hb J (about 55%) and Hb A and had two alpha loci per chromosome. His restriction site map was found to be identical to that obtained with a normal DNA, except for a mutant Bgl II site which was observed on the Hb J chromosome proximal to the 5' alpha-locus. We have also mapped the DNA of a compound heterozygote for Hb J and alpha-thalassemia, who synthesizes 38% Hb J and we have found a single alpha gene corresponding to a - alpha 3.7 haplotype on one chromosome and two alpha genes, respectively alpha J and alpha A, on the other.
D001345:Autoradiography; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000235:genetics; D005260:Female; D005809:Genes, Regulator; D005914:Globins / Q000235:genetics*; D006448:Hemoglobin J / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D013789:Thalassemia / Q000235:genetics
D006455:Hemoglobins, Abnormal; D006448:Hemoglobin J; D005914:Globins; D004262:DNA Restriction Enzymes