title: Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody.
abstract: Glycoproteins gD-1 and gD-2 of herpes simplex virus types 1 and 2, respectively, were purified on an immunoadsorbent consisting of the type-common monoclonal antibody HD-1 linked to Sepharose. Each glycoprotein was of sufficient purity, quantity, and biological activity to be used for immunological and biochemical studies. Each glycoprotein induced high titers of type-common monospecific neutralizing antibody in mice. Amino aicd analysis indicated that gD-1 and gD-2 had similar though not identical amino acid compositions.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D007163:Immunosorbent Techniques; D010455:Peptides / Q000032:analysis; D018139:Simplexvirus / Q000032:analysis*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D010455:Peptides; D014759:Viral Envelope Proteins; D014764:Viral Proteins