Exposure of aromatic residues of Streptomyces subtilisin inhibitor. A photo-CIDNP study.
Exposure of aromatic residues, Tyr 7, Tyr 75, Tyr 93, His 43, His 106, and Trp 8, was studied by laser-induced photo-CIDNP in the 1H NMR spectrum of Streptomyces subtilisin inhibitor at 360 MHz. Only Tyr 7 and Tyr 75 gave strong CIDNP signals, whereas the rest of the aromatic residues gave no detectable signals in the temperature range 25-55 degrees C. From the temperature dependence data, it is concluded that Tyr 7 is well exposed at all temperatures, whereas the exposure of Tyr 75 increases with temperature, in agreement with the conclusion obtained by other methods. Agreements and discrepancies between the conclusions derived from the CIDNP data and the results so far obtained by other methods are compared for all the aforementioned aromatic residues.
D001426:Bacterial Proteins; D055598:Chemical Phenomena; D002621:Chemistry; D006639:Histidine; D007834:Lasers; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D013381:Subtilisins / Q000037:antagonists & inhibitors*; D013696:Temperature; D014357:Trypsin; D014443:Tyrosine
D001426:Bacterial Proteins; D011522:Protons; C018150:subtilisin inhibitor protein, Streptomyces; D014443:Tyrosine; D006639:Histidine; D013381:Subtilisins; D014357:Trypsin