[Enzootic bovine leukosis application of E.L.I.S.A. technique for the detection of antibodies].
A simple microplate method of enzyme-linked immunosorbent assay (E.L.I.S.A.) for detecting antibodies to bovine leukosis virus (B.L.V.) is described. The antigen consisted of a solution containing the two major antigens of the B.L.V. (gp 51 and p 24) obtained by a technique of purification using CN-Br activated Sepharose 4B. This E.L.I.S.A. was compared with the agar gel immunodiffusion test (A.G.I.D.T.) in a study of 545 bovine sera. The total discrepancy rate between the two tests was 11% with a better sensitivity for E.L.I.S.A.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000302:isolation & purification; D002417:Cattle / Q000276:immunology*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral
Leucose bovine enzootique application de la technique E.L.I.S.A. à la détection des anticorps.