Investigations on myelination in vitro: thyroid hormone receptors in cultures of cells dissociated from embryonic mouse brain.
Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are suitable for studying the regulation of myelination by T3 in vitro, display a high specificity for L-T3 in that the receptors for L-T3 do not bind D-T3, D-thyroxine, L-diiodothyronine, or DL-thyronine, and bind only small amounts of L-thyroxine.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000032:analysis; D002478:Cells, Cultured; D003600:Cytosol / Q000032:analysis; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis*; D011988:Receptors, Thyroid Hormone; D013970:Thyronines / Q000378:metabolism; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism
D011956:Receptors, Cell Surface; D011988:Receptors, Thyroid Hormone; D013970:Thyronines; D014284:Triiodothyronine; D013974:Thyroxine