[Peculiarities of specific binding of serotonin by blood leukocytes, peritoneal cells and synaptosomes of mice].
The properties of serotonin-active sites were studied on peritoneal cells, blood leukocytes and synaptosomes of mice (CBA line). Treatment of cell suspensions with EDTA, ouabain, strophanthin, 2,4-dinitrophenol, dithiothreitol and trypsin demonstrated that serotonin binding by peritoneal cells and leukocytes depends on bivalent ions and K+, Na+-pump operation, requires energy and intact disulfide bonds and is determined by a protein structure. ATP and ADP were found to inhibit amine adsorption by peritoneal cells. These cells specifically bind ATP 10 times more intensively than leukocytes. The data obtained are suggestive of differences in the composition of serotonin-active structures of blood leukocytes, peritoneal cells and synaptosomes.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
D011985:Receptors, Serotonin; D012701:Serotonin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase
Osobennosti spetsificheskogo sviazyvaniia serotonina leĭkotsitami krovi, peritoneal'nymi kletkami i sinaptosomami mysheĭ.