title: Immunohistochemical and biochemical studies on collagen types in angiofibromas and shagreen patches from patients with tuberous sclerosis.
abstract: The distribution of collagen types in angiofibroma and shagreen patch from patients with tuberous sclerosis was examined biochemically and immunohistochemically by indirect immunofluorescence microscopy using type-specific anti-collagen antibodies. Types I and III collagens were distributed in the interstitium of whole tumor tissues of angiofibriomas and a shagreen patch, showing relatively fine fibers at the periphery and large bundles in the center. No significant difference in the distribution of type I and III collagens was observed. Densitometric analysis of gel electrophoresis patterns of collagens solubilized from the skin by limited pepsin digestion revealed that both type I and III collagens were the main constituents of the diseased regions of the skin with tuberous sclerosis, and the ratio of type I collagen/type III collagen was relatively constant, which was not contradictory to the results of immunofluorescence microscopy. The presence of an unidentified component, probably type V collagen, was observed in the angiofibroma tissue from one patient with tuberous sclerosis.
mesh_terms: D000293:Adolescent; D000328:Adult; D003094:Collagen / Q000378:metabolism*; D003238:Connective Tissue / Q000473:pathology; D005153:Facial Neoplasms / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D018219:Histiocytoma, Benign Fibrous / Q000473:pathology*; D006801:Humans; D009506:Nevus / Q000473:pathology*; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology*; D014402:Tuberous Sclerosis / Q000473:pathology*
chemical_list: D003094:Collagen