Aromatic hydrocarbon metabolites in fish: automated extraction and high-performance liquid chromatographic separation into conjugate and non-conjugate fractions.
An automated extractor-concentrator was used to extract metabolites of naphthalene, 2,6-dimethylnaphthalene, and benzo[a]pyrene from serum, bile and liver homogenate of rainbow trout (Salmo gairdneri). The extracts were analyzed by reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. Recoveries of naphthalene and 2,6-dimethylnaphthalene metabolites from all matrices were generally greater than 90%; however, the recoveries of benzo[a]pyrene metabolites from serum ranged from 37-99%. In addition, conjugated metabolites of polycyclic aromatic hydrocarbons (PAHs) were separated from non-conjugated metabolites and parent PAHs by using two diol columns with normal-phase HPLC. The extraction and separation techniques were also applied to isolate metabolites in samples from fish fed 2,6-dimethylnaphthalene.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000097:blood / Q000378:metabolism*; D001646:Bile / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D008099:Liver / Q000032:analysis; D009281:Naphthalenes / Q000097:blood / Q000378:metabolism*
D001580:Benzopyrenes; D009281:Naphthalenes; D001564:Benzo(a)pyrene; C028519:2,6-dimethylnaphthalene