title: An inhibitor of collagenase from human amniotic fluid. Purification, characterization and action on metalloproteinases.
abstract: 1. An inhibitor of collagenase of apparent mol.wt. 28000 was isolated from term human amniotic fluid. 2. It is active against mammalian collagenases from a number of species and tissues as well as other mammalian metalloproteinases, but has no activity against bacterial metalloproteinases. 3. Activity is destroyed by treatment with either trypsin or 4-aminophenylmercuric acetate, by heat, and by reduction and carboxymethylation. 4. All the properties observed suggest that it is similar to the synthesized tissue inhibitor of metalloproteinases.
mesh_terms: D000627:Aminophenols / Q000494:pharmacology; D000653:Amniotic Fluid / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D008666:Metalloendopeptidases; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors*; D011247:Pregnancy; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D014357:Trypsin
chemical_list: D000627:Aminophenols; D004791:Enzyme Inhibitors; D007461:Iodoacetates; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D008623:Mercaptoethanol; D014357:Trypsin; D008666:Metalloendopeptidases; D003012:Microbial Collagenase