title: Nucleoside triphosphate phosphohydrolase activities in African swine fever virus.
abstract: African swine fever virus contains nucleoside triphosphate phosphohydrolase activity which releases 32P phosphate from gamma-32P ATP at a rate of about 1 mumol/h mg of virus protein. The hydrolase activity is slightly stimulated by adding nucleic acids to the reaction mixture and under conditions of RNA synthesis. A study of the rate of ATP hydrolysis at different concentrations of ATP suggests the existence of two phosphohydrolase activities with apparent Km values of about 0.04 and 1 mM.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000358:African Swine Fever Virus / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007497:Iridoviridae / Q000201:enzymology*; D007700:Kinetics; D043583:Nucleoside-Triphosphatase; D009711:Nucleotides / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D009711:Nucleotides; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D010744:Phosphoric Monoester Hydrolases; D043583:Nucleoside-Triphosphatase