An improved filtration procedure for measuring opiate receptors in small regions of rat brain.
A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 micrograms of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D005374:Filtration / Q000379:methods; D009270:Naloxone / Q000378:metabolism; D051381:Rats; D011957:Receptors, Opioid / Q000032:analysis*
D011957:Receptors, Opioid; D009270:Naloxone