[Analysis of insertions of the determinant of drug resistance to kanamycin and chloramphenicol into bacteriophage lambda att80].
The insertion sites of elements Tn9 and Tn601 which determine chloramphenicol and kanamycin resistance have been detected restriction analysis. The functioning of transposons i.e. their stability or instability, has been found to influence the specificity of their insertions into the genome of lambda att80 bacteriophage. During transposition from stable integration sites both transposons are inserted into the regions of the lambda att80 bacteriophage genome, definite for each transposon. However, during transposition from the site of unstable integration both determinants of drug resistance are inserted into different regions of the phage genome.
D010582:Bacteriophage lambda / Q000235:genetics*; D002701:Chloramphenicol / Q000037:antagonists & inhibitors*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000235:genetics; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial; D005814:Genes, Viral; D007612:Kanamycin / Q000037:antagonists & inhibitors*; D012093:Replicon
D004251:DNA Transposable Elements; D004279:DNA, Viral; D007612:Kanamycin; D002701:Chloramphenicol; D004262:DNA Restriction Enzymes
Analiz vnedreniĭ determinant lekarstvennoĭ ustoĭchivosti k kanamitsinu i khloramfenikolu v bakteriofag lambda att80.