A single-step centrifugation method for separation of granulocytes and mononuclear cells from blood using discontinuous density gradient of Percoll.
A rapid and reproducible method is described for the simultaneous purification of mononuclear and polymorphonuclear cells, based on the use of a discontinuous gradient of Percoll. Centrifugation at 600 X gav for 20 min resulted in separation of the mononuclear and polymorphonuclear cells into 2 distinct bands at the interfaces. The upper layer contained the mononuclear cells and the lower band highly purified polymorphonuclear cells. Both populations were purified about 97% without detectable cell alteration.
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes; D006098:Granulocytes; D006801:Humans; D007962:Leukocytes; D009504:Neutrophils; D010586:Phagocytes; D011205:Povidone / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone