Rapid methods for extraction and concentration of poliovirus from oyster tissues.
A procedure is discussed for the extraction of poliovirus from oyster meats by modification of several enterovirus extraction techniques. The modified method uses meat extract and Cat-Floc, a polycationic electrolyte, for virus extraction and concentration. Virus recovery from inoculated oyster homogenates is 93-120%. Adsorption of viruses to oyster proteins by acidification of homogenates does not affect virus recovery. Elution of viruses from oyster proteins appears more efficient at pH 9.5 than at pH 8.0. This technique is relatively simple, economical and requires only 2.5 h to complete the combined extraction and concentration procedure.
D000818:Animals; D004770:Enterovirus / Q000302:isolation & purification; D005506:Food Contamination; D006863:Hydrogen-Ion Concentration; D008460:Meat; D008722:Methods; D010125:Ostreidae / Q000382:microbiology*; D017955:Poliovirus / Q000302:isolation & purification*; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D014020:Tissue Extracts
D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D014020:Tissue Extracts; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride