Effects of dibutyryl-cANP on glycolysis in washed human erythrocytes.
The experiments were carried out with washed human erythrocytes in order to study the effects of dibutyryl-cAMP (DB-cAMP) on glycolysis. 5 mM DB-cAMP significantly increases glucose consumption and lactate production in incubated erythrocytes. The cross-over plot of glycolytic intermediates shows that increased glycolysis is probably the result of activation of phosphofructokinase by DB-cAMP. Under the same condition DB-cAMP significantly protects the 2,3-diphosphoglycerate level in incubated erythrocytes.
D019794:2,3-Diphosphoglycerate; D001786:Blood Glucose / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000097:blood
D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D007773:Lactates; D019794:2,3-Diphosphoglycerate; D003994:Bucladesine