title: Chemical characteristics of the L-glutamate receptor on the Onchidium neuron.
abstract: Application of L-glutamate (L-glu) to particular neurons (G-H cells) in the esophageal ganglia of Onchidium produced hyperpolarization associated with an increase in K+ permeability. The reversal potential (Erev) for this response, was -60 mV. The ED50, an indicator of affinity between L-glu and its receptive site, was 5.3 mM, and the Hill coefficient n, an indicator of cooperativity, was 2. Other chemicals, structurally analogous to L-glu, were also examined on G-H cells. The responses of L-aspartate were similar to those of L-glu with respect to Erev, ED50, and n. However, the G-H cells responded differently to D-glutamate (D-glu) and kainic acid. The D-glu response had an Erev = -37.5 mV, ED50 = 29.4 mM, and n = 1; that of kainic acid had an Erev = 0 mV, ED50 = 1.5 mM, and n = 1. L-glutamine and GABA elicited very small responses, amplitudes of which were less than 10% of the L-glu response. The structure-activity relationship between L-glu and its analogues, estimated from Erev, ED50, and Hill coefficient during each drug application, indicates that the presence of alpha-NH2, alpha-COOH, and gamma-COOH groups is essential for the L-glu response and that the response characteristics may be closely related to the relative positions of these groups.
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004947:Esophagus / Q000294:innervation; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D006372:Helix, Snails; D007608:Kainic Acid / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008974:Mollusca; D009474:Neurons / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000502:physiology*; D017470:Receptors, Glutamate
chemical_list: D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D005973:Glutamine; D001224:Aspartic Acid; D011188:Potassium; D007608:Kainic Acid