title: The effect of sodium channel activators on muscarinic receptors of neuroblastoma cells.
abstract: Incubation of neuroblastoma NIE 115 cells with veratrine leads to an apparent reduction in the number of muscarinic acetylcholine receptors assayed by [3H]scopolamine methyl chloride binding. No true down-regulation of the receptors occurs but a component of veratrine with muscarinic receptor affinity, which is not veratridine, enters the intracellular water space during the incubation period and competes with [3H]scopolamine methyl chloride for the muscarinic binding sites in subsequent ligand binding assays unless it is carefully washed away. Treatment of cells with the agonist carbamoylcholine does, however, lead to a true downregulation of muscarinic receptors.
mesh_terms: D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D007473:Ion Channels / Q000378:metabolism*; D009447:Neuroblastoma; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects*; D012602:Scopolamine Derivatives / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D014701:Veratridine / Q000494:pharmacology; D014702:Veratrine / Q000494:pharmacology*
chemical_list: D007473:Ion Channels; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D014701:Veratridine; D002217:Carbachol; D012964:Sodium; D014702:Veratrine