Interaction of brain synaptic vesicles induced by endogenous Ca2+ -dependent phospholipase A2.
Endogenous phospholipase A2 activity of brain synaptic vesicles was Ca2+ -dependent and was increased by prostaglandin F2 alpha, calmodulin, adenosine 3', 5' -monophosphate, and adenosine triphosphate, whereas the activity was inhibited by prostaglandin E2 in the absence or presence of calmodulin. Light-scattering measurements demonstrated that stimulation of the enzyme's activity correlated with the induction of vesicle-vesicle aggregation. The effects of these compounds on endogenous synaptic vesicle phospholipase A2 activity may imply a common end point of their purported neuromodulatory actions, and indicate that synaptic vesicle phospholipase A2 may play a central role in presynaptic neurotransmission.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001921:Brain / Q000502:physiology* / Q000648:ultrastructure; D002118:Calcium / Q000502:physiology*; D002474:Cell-Free System; D000242:Cyclic AMP / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005089:Exocytosis; D008561:Membrane Fusion; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000502:physiology*
D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium