Failure of ethanol or isopropanol pretreatment to affect carbon tetrachloride-induced inhibition of hepatic microsomal calcium pump activity.
The calcium pump of liver microsomes is sensitive to CCl4 metabolism in vitro and in vivo. Treatment of rats with alcohol might involve an enhancement of the action of low doses of CCl4 on rat liver microsomal calcium pump. It was found that treatment of fasted rats with isopropanol or ethanol potentiated the increase of serum glutamic oxaloacetic transaminase elicited by CCl4. Alcohol pretreatment alone had no effect on calcium pump activity. After CCl4 alone, the calcium pump was 50% inhibited at 20 min, and 85% inhibited at 1 hr. Pretreatment with either alcohol had no effect on either rate of decline or extent of CCl4-dependent depression of the liver microsomal calcium pump. The mechanism of alcohol potentiation of CCl4-induced hepatotoxicity probably resides in alteration of processes developing after the initial events of CCl4 metabolism.
D000433:1-Propanol / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005215:Fasting; D007473:Ion Channels / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007473:Ion Channels; D000431:Ethanol; D000433:1-Propanol; D001219:Aspartate Aminotransferases; D002118:Calcium