Expression of hepatitis B virus surface antigen gene in cultured cells by using recombinant plasmid vectors.
By using a new host-vector system, expression of the gene coding for hepatitis B surface antigen has been studied. A subgenomic fragment of cloned hepatitis B viral DNA was inserted into the plasmid vector pSV010. Transfection of COS cells with the recombinant plasmid vector containing hepatitis sequences leads to the synthesis of hepatitis B surface antigen, which is released in the culture medium in the form of 22-nm particles similar to those found in the sera of hepatitis carriers.
D000818:Animals; D002478:Cells, Cultured; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D000882:Haplorhini; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D004274:DNA, Recombinant; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens