Antiserum to SV40 T antigens from a rabbit inoculated with autochthonous cells transformed in vitro by SV40.
A morphologically transformed cell line was established after infection with SV40 of the primary culture derived from a kidney removed surgically from a rabbit. The transformed cells were free of detectable infectious SV40 particles but produced them upon fusion with permissive monkey cells, and produced SV40-specific large and small T antigens. Repeated subcutaneous inoculations of the transformed cells into the animal from which the parental kidney cells had been derived failed to result in tumor formation but raised a substantial level of antibodies to the SV40-specific large and small T antigens as well as to the 58,000 dalton molecular weight cellular protein (middle T antigen).
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification*; D000957:Antigens, Viral, Tumor; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D007106:Immune Sera; D007668:Kidney / Q000276:immunology; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D007106:Immune Sera