Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.
Thirty-three of 50 noncultivable human rotavirus strains from a variety of locations were successfully rescued by gene reassortment. The serotype of each of the 33 strains was investigated by a qualitative cytopathic effect neutralization assay. Nineteen strains resembled the previously characterized human rotavirus serotype Wa, whereas three strains were serologically related to the DS-1 strain. Eleven strains appeared to be serotypically distinct from the Wa and DS-1 strains and thus apparently represent one or more new human rotavirus serotypes.
D003588:Cytopathogenic Effect, Viral; D005814:Genes, Viral; D006801:Humans; D008722:Methods; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000254:growth & development*; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D012703:Serotyping; D010948:Viral Plaque Assay
D012367:RNA, Viral