Capillary gas chromatographic method for the analysis of lignans in human urine.
We describe a capillary column gas chromatographic (GC) method for the analysis of lignans in urine. Lignans are excreted as mono-glucuronides which are first extracted on a small reversed-phase cartridge of octadecylsilane bonded silica (Sep-Pak C18) and thereafter isolated by anion exchange chromatography on DEAE-Sephadex A-25, prepared in the acetate form. Lignan mono-glucuronides are enzymatically hydrolysed and re-extracted on a Sep-Pak C18 cartridge. Quantification is carried out by capillary column GC of the trimethylsilyl ether derivatives. The specificity of the method was checked by GC/MS and the intra-assay coefficient of variation (CV) for the two lignans, enterolactone (trans-2,3-bis(3-hydroxybenzyl)butyrolactone) and enterodiol (2.3-bis(3-hydroxybenzyl)butane-1,4-diol) varied between 5 and 8%. Some values for the excretion of these lignans by normal men and women are presented.
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000652:urine; D000328:Adult; D002072:Butylene Glycols / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D002852:Chromatography, Ion Exchange; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000652:urine; D006801:Humans; D017705:Lignans; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000652:urine*
D002072:Butylene Glycols; D005965:Glucuronates; D017705:Lignans; D010936:Plant Extracts; C029498:2,3-bis(3'-hydroxybenzyl)butane-1,4-diol; D015107:4-Butyrolactone; C029497:2,3-bis(3'-hydroxybenzyl)butyrolactone