Bicuculline-sensitive GABA receptors in rat ovary.
A specific [3H]GABA binding site, characterized by a kinetic constant of 52 nM and by a maximal binding capacity of 17 fmol/mg protein was identified in a membrane preparation of rat ovary. [3H]GABA binding was displaced by muscimol, unlabelled GABA or bicuculline. The accumulation of cyclic AMP in the slices of rat ovary was strongly increased by GABA or muscimol and these effects were antagonized by picrotoxin or bicuculline. Our data suggest a possible role of GABA receptors in the regulation of ovarian function in the rat.
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis; D005260:Female; D066298:In Vitro Techniques; D010053:Ovary / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis / Q000187:drug effects*; D011963:Receptors, GABA-A
D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D000242:Cyclic AMP; D001640:Bicuculline