Simian virus 40 mutant with transposed T-antigen and VP1 genes.
A simian virus 40 mutant with the T-antigen gene transposed to the late region of the viral genome has been constructed. This transposed molecule directed the synthesis of a full-sized, 92,000-dalton large T antigen in both permissive and nonpermissive cells. This large T antigen functioned in the initiation of viral DNA replication and in the transformation of nonpermissive cells. T-antigen synthesis by this transposed genome had the characteristics of late transcription, thus indicating that functional large T antigen of simian virus 40 is not required for the initiation of late transcription.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000502:physiology; D000957:Antigens, Viral, Tumor; D002472:Cell Transformation, Viral; D004261:DNA Replication; D005786:Gene Expression Regulation; D005814:Genes, Viral; D051379:Mice; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins