The production of varicella Zoster virus antiserum in laboratory animals. Brief report.
Hyperimmune anti-varicella zoster virus (VZV) antisera were prepared in BALB/c mice, Lewis rats and New Zealand rabbits by subcutaneous inoculations of purified VZ virions suspended in Freund's adjuvant. VZV antibodies were demonstrated both by an enzyme-linked immunosorbent assay (ELISA) and by indirect immunofluorescence (IF). VZV could not be detected in organs of any inoculated animals by IF or by cocultivation of tissue fragments from infected animals with human diploid fibroblasts.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant; D014645:Herpesvirus 3, Human / Q000276:immunology* / Q000502:physiology; D007106:Immune Sera; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D014779:Virus Replication
D000914:Antibodies, Viral; D007106:Immune Sera; D005620:Freund's Adjuvant