Separation of penicillin and its major degradation products by ion-pair reversed-phase high-pressure liquid chromatography.
An ion-pair reversed-phase high-pressure liquid chromatographic technique capable of separating penicillin and its major degradation products within 8 min was developed. The influence of pH, counterion concentration, buffer concentration, and organic modifier content was studied and the observed behavior of the compounds during the chromatographic process was discussed.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D010397:Penicillanic Acid / Q000032:analysis / Q000031:analogs & derivatives; D010400:Penicillin G / Q000031:analogs & derivatives*; D010406:Penicillins / Q000302:isolation & purification*
D010406:Penicillins; C008782:penicilloic acid; D010397:Penicillanic Acid; C017609:penilloic acid; D010400:Penicillin G