Transcription of the beta-like globin genes and pseudogenes of the goat in a cell-free system.
We have examined the transcription in a cell-free system of all the members of a highly regulated gene family, the beta-like hemoglobin genes of the goat. These five genes, which code for embryonic, fetal, juvenile, and adult beta-globin proteins, are all transcribed to roughly the same extent in the in vitro system. In all cases initiation of transcription is accurate. However, two goat beta-globin pseudogenes, as well as several artificially constructed deletion mutants, are not transcribed in vitro. A common feature of the transcriptionally inactive genes is the lack of an AT-rich consensus sequence just upstream of the presumptive initiation site.
D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D009154:Mutation; D014158:Transcription, Genetic
D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes