Characterization of SV40-transformed human liver cells before and after passage through crisis.
SV40-transformed human liver cells could be passed through crisis after transfection with various DNAs (SV40, BKV, or salmon sperm). The pre- and post-crisis cells were compared with respect to growth behavior and the state of the viral genome. Comparison of the viral inserts in the pre- and post-crisis cells showed minor changes in the integration pattern of the SV40 DNA after recovery from crisis.
D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008099:Liver / Q000254:growth & development; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D004279:DNA, Viral