title: The distribution of enzymes which synthesize nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate in monkey, rabbit, and ground squirrel retinas.
abstract: The activity of adenosinetriphosphate:nicotinamide adenylyltransferase (EC 2.7.7.1) was measured in all the layers of monkey, rabbit, and ground squirrel retinas. Nicotinamide adenine dinucleotide (NAD) kinase (EC 2.7.1.23) distribution was measured in monkey and rabbit retinas. An attempt was made to measure NAD synthetase (EC 6.3.5.1), but the activities in the retinal layers were too low to produce a reliable increment in the levels of endogenous NAD. In monkey retina the adenylyl transferase was highest by far in the outer and inner nuclear layers, lower and variable in ganglion cell and fiber layers, and almost absent elsewhere. Rabbit retina differed in that activity was nearly absent in the outer nuclear layer, whereas in the ground squirrel outer nuclear layer activity was double that of the inner nuclear layer. The species differences suggest that adenylyl transferase is almost absent from cone cell nuclei and high in rod cell nuclei. NAD kinase distribution in monkey retina was almost the mirror image of that of adenylyl transferase.
mesh_terms: D000818:Animals; D007700:Kinetics; D008252:Macaca fascicularis; D009243:NAD / Q000096:biosynthesis*; D009249:NADP / Q000096:biosynthesis*; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011817:Rabbits; D012160:Retina / Q000201:enzymology*; D012589:Sciuridae; D013045:Species Specificity
chemical_list: D009243:NAD; D009249:NADP; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase; D009713:Nucleotidyltransferases; D009612:Nicotinamide-Nucleotide Adenylyltransferase