Polyamine-induced hydrolysis of apurinic sites in DNA and nucleosomes.
The ability of different polyamines to catalyze hydrolysis of phosphodiester linkages in apurinic and apyrimidinic (AP) sites has been investigated in supercoiled, relaxed and denatured DNA, and also in core and chromatosome particles. The rate constants for the hydrolysis in the DNAs have been determined. In general the order of effectiveness of the polyamines were: spermine greater than spermidine greater than putrescine greater than cadaverine. A 9 fold difference in rate constants was found between spermine and cadaverine. No difference in the rate of hydrolysis was seen between AP-sites in supercoiled and relaxed DNAs, whereas the rate for the single-stranded DNA and DNA in core and chromatosome particles was only half of that in the double-stranded DNA. All AP-sites in both free DNA and DNA-histone particles were hydrolyzed in the presence of polyamines. For all polyamines, with the exception of spermine, increasing concentration of both Mg++ and salts such as KCl both led to a large decrease in the rate of polyamine-induced hydrolysis of AP-sites. The rate of hydrolysis increased markedly with increasing pH in the pH range pH 6 - pH 11.
D000818:Animals; D001080:Apurinic Acid; D002103:Cadaverine; D002413:Cations, Divalent; D002460:Cell Line; D004247:DNA; D004720:Endonucleases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D051379:Mice; D009707:Nucleosomes / Q000032:analysis*; D009994:Osmolar Concentration; D010954:Plasmacytoma; D011073:Polyamines; D011119:Polynucleotides; D011700:Putrescine; D013095:Spermidine; D013096:Spermine; D013329:Structure-Activity Relationship
D002413:Cations, Divalent; D009707:Nucleosomes; D011073:Polyamines; D011119:Polynucleotides; D001080:Apurinic Acid; D013096:Spermine; D004247:DNA; D004720:Endonucleases; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine