[Physicochemical properties of Teschen disease virus RNA].
The specific infectivity of virion RNA of teschen disease virus in a sensitive PP cell culture was 4-5 lg TCD50/ml per 1 microgram RNA. When virion RNA was inoculated into cell cultures insusceptible to the native virus, the virus replicated to a titre of 2.0-3.5 lg TCD50/ml. The molecular weight of virion RNA determined by two independent methods was 2.7 x 10(6) daltons. Tm calculated from the curve of virion RNA melting temperature was 57 degrees C. The double-stranded replicative form of RNA recovered from virus-infected PP cells was shown to have sucrose gradient sedimentation coefficient of 20 S. The specific infectivity was 2-3 lg TCD50/ml per 1 microgram of RNA.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D004770:Enterovirus / Q000032:analysis*; D004771:Enteroviruses, Porcine / Q000032:analysis*; D008970:Molecular Weight; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D014776:Virus Cultivation
D012367:RNA, Viral
Nekotorye fiziko-khimicheskie svoĭstva RNK virusa bolenzi Teshnena.