On the mechanism of enhanced monocyte and neutrophil cytotoxicity in severe psoriasis.
Monocyte and neutrophil function assessed as antibody-dependent cell-mediated cytotoxicity (ADCC) using IgG-sensitizing human erythrocytes as target cells was enhanced in patients with severe psoriasis when compared to healthy controls. We found significant correlation between increased monocyte ADCC and increased neutrophil ADCC, No differences in basal cAMP levels and cAMP responses during initiation of the ADCC reaction was observed between psoriatics and normals. Also degranulation determined as lysozyme release during ADCC was normal. In contrast, the increase in ADCC was significantly correlated to an enhanced hexose monophosphate shunt activation in the effector cells during the cytotoxic reaction. Activity of enzymes responsible for the respiratory burst was not altered in psoriasis since superoxide production after stimulation with phorbol myristate acetate was normal. Likewise, oxygen consumption and degranulation following phagocytosis of opsonized zymosan particles in neutrophils was found normal in psoriasis. Since monocytes showed increased binding of IgG-sensitized erythrocytes these data indicate that the enhanced monocyte and neutrophil ADCC is caused by an enhancement of the respiratory burst possibly induced by increased Fc receptor activity.
D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D000242:Cyclic AMP / Q000097:blood; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006600:Hexosephosphates / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D010101:Oxygen Consumption; D010545:Peroxides / Q000096:biosynthesis; D010587:Phagocytosis; D011565:Psoriasis / Q000097:blood*; D012397:Rosette Formation
D006600:Hexosephosphates; D010545:Peroxides; D000242:Cyclic AMP