Effect of growth arrest on the doubling potential of human fibroblasts in vitro: a possible influence of the donor.
Population doublings versus time in culture were compared in human postnatal skin fibroblasts from normal donors, a cancer patient, and from donors suffering from Cockayne syndrome, Ataxia telangiectasia, and Fanconi's anemia (FA). Confluent cultures were maintained in a nonproliferating state for 14 to 27 d in 0.5% serum medium. The results show that the ability of cells to resume division after a resting stage can be influenced by pathologic conditions. In arrested FA cell populations an increase of the population doublings and of the calendar time were observed. It is possible that in some cell populations the resting stage favors the expression of growth potentialities related to instability of the cells.
D001260:Ataxia Telangiectasia / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003057:Cockayne Syndrome / Q000473:pathology; D005199:Fanconi Anemia / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007399:Interphase; D008113:Liver Neoplasms / Q000473:pathology; D008875:Middle Aged; D012867:Skin / Q000166:cytology; D013997:Time Factors