Herpes simplex virus type-1 glycoprotein D gene: nucleotide sequence and expression in Escherichia coli.
The protein coding region of the herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D010455:Peptides / Q000235:genetics; D021382:Protein Sorting Signals; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines
D000956:Antigens, Viral; D006023:Glycoproteins; D010455:Peptides; D021382:Protein Sorting Signals; D014764:Viral Proteins; D014765:Viral Vaccines