title: Cloning of the aerobactin-mediated iron assimilation system of plasmid ColV.
abstract: The high-affinity iron assimilation system of plasmid ColV-K30 was cloned on the vector plasmid pPlac. Plasmid pABN1 was isolated by means of sensitivity to cloacin, a bacteriocin using the same outer membrane receptor as ferric aerobactin. Restriction maps were determined for this plasmid and for a subclone, pABN5. Plasmid pABN1 codes for the complete gene complex, whereas plasmid pABN5 encodes only the biosynthetic genes for aerobactin. Regulation of the uptake system by iron is retained in cloned sequences of pABN1.
mesh_terms: D001425:Bacterial Outer Membrane Proteins; D003086:Bacteriocin Plasmids; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006877:Hydroxamic Acids / Q000096:biosynthesis; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents; D010957:Plasmids; D011956:Receptors, Cell Surface / Q000235:genetics*; D017262:Siderophores
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D011956:Receptors, Cell Surface; D017262:Siderophores; C035902:siderophore receptors; C031819:aerobactin; D007501:Iron