The use of affinity chromatography on 2'5' ADP-sepharose reveals a requirement for NADPH, thioredoxin and thioredoxin reductase for the maintenance of high protein synthesis activity in rabbit reticulocyte lysates.
D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D001798:Blood Proteins / Q000096:biosynthesis*; D002846:Chromatography, Affinity; D000242:Cyclic AMP / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000502:physiology*; D009249:NADP / Q000502:physiology*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D012685:Sepharose / Q000031:analogs & derivatives; D013403:Sugar Phosphates / Q000502:physiology; D013880:Thioredoxin-Disulfide Reductase / Q000502:physiology*; D013879:Thioredoxins / Q000502:physiology*
C025246:2',5'-ADP-sepharose; D001426:Bacterial Proteins; D001798:Blood Proteins; D013403:Sugar Phosphates; D013879:Thioredoxins; D009249:NADP; D006160:Guanosine Triphosphate; D012685:Sepharose; D000242:Cyclic AMP; D009247:NADH, NADPH Oxidoreductases; D013880:Thioredoxin-Disulfide Reductase; D005978:Glutathione