The B95-8 isolate of Epstein-Barr virus arose from an isolate with a standard genome.
Blot hybridization studies revealed that the deletion which characterizes the DNA from the B95-8 strain of Epstein-Barr virus was not present in the virus from which the B95-8 strain was derived (883L). The deletion event must have occurred during establishment of the B95-8 cell line or very soon afterward, since the deletion was present in Epstein-Barr virus DNA from a cell line established with B95-8 virus soon after it became available. The presence of the deletion correlates with decreased expression of the gp220 viral envelope glycoprotein.
D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes; D008970:Molecular Weight; D009154:Mutation; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes