title: Eco RI restriction-site polymorphism of the albumin gene in different inbred strains of rat.
abstract: Two types of variant EcoRI restriction enzyme patterns of albumin-gene DNA fragments have been detected in different rat strains by agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat albumin cDNA probes. The type I albumin gene variant is characteristic of the Sprague-Dawley strain, and type II is found in Buffalo rats. The occurrence of these variants is interpreted as the result of simple allelic polymorphism because they are inherited in a normal Mendelian fashion when crossing Sprague-Dawley and Buffalo rats. The distribution of the two genetic variants in various inbred strains of rat suggests that type I represents the original or ancestral form of the albumin gene and that type II appeared spontaneously during laboratory breeding.
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015246:Deoxyribonuclease EcoRI; D005260:Female; D005796:Genes; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D051381:Rats; D011914:Rats, Inbred BN / Q000235:genetics; D011915:Rats, Inbred BUF / Q000235:genetics; D011917:Rats, Inbred Lew / Q000235:genetics; D011919:Rats, Inbred Strains / Q000235:genetics*; D012709:Serum Albumin / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D012709:Serum Albumin; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI