Virion DNA of ground squirrel hepatitis virus: structural analysis and molecular cloning.
The structure of the encapsidated DNA genome of ground squirrel hepatitis virus (GSHV) has been examined by restriction endonuclease cleavage, nucleic acid hybridization, and molecular cloning. GSHV virion DNA is a relaxed circular molecule of approximately 3,200 bases in length; most molecules harbor an extensive single-stranded region which is largely confined to one-half of the genome. The full-length viral DNA strand is covalently bound to protein. The single-stranded region can be repaired in vitro by the action of the endogenous virion polymerase, exogenously added DNA polymerase from avian myeloblastosis virus, or both. Restriction enzyme cleavage of viral DNA from different isolates demonstrated that multiple variants of GSHV exist in nature. The genomes of two such strains have been cloned in Escherichia coli, and their physical maps have been determined. Nucleic acid hybridization studies revealed that the strains share sequence homology with the DNA of human hepatitis B virus. Regions homologous to the coding regions for the surface and core antigens of human hepatitis B virus have been localized on the GSHV chromosome. Molecular cloning experiments have also led to the identification of a region of the viral genome which is altered in a procaryotic host.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000032:analysis* / Q000378:metabolism; D005814:Genes, Viral; D006518:Hepatitis Viruses / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012589:Sciuridae; D014764:Viral Proteins / Q000378:metabolism; D014771:Virion / Q000235:genetics
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes