Detection of anti-rotavirus antibody-producing cells in paraffin-embedded tissue sections.
An assay to detect anti-rotavirus antibody-producing cells was developed in rabbits. Sections of alcohol-fixed, paraffin-embedded tissues were used to make an immunofluorescent "sandwich" consisting of specific antibody-producing cells, a concentrated suspension of bovine rotavirus, and rhodamine-conjugated anti-rotavirus serum. Rhodamine-stained, anti-rotavirus antibody-producing cells could be simultaneously stained by a fluorescein-conjugated antiserum reactive to rabbit immunoglobulin.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007114:Immunization; D007136:Immunoglobulins / Q000096:biosynthesis; D008198:Lymph Nodes / Q000166:cytology; D010232:Paraffin; D011817:Rabbits; D012087:Reoviridae / Q000276:immunology*; D012235:Rhodamines; D012401:Rotavirus / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D007136:Immunoglobulins; D012235:Rhodamines; C009434:tetramethylrhodamine isothiocyanate; D010232:Paraffin