[Spontaneous cytomegalovirus infection of kidney cell cultures of green monkeys].
In subcultivation of 104 batches of primary kidney cell cultures from clinically normal green monkeys 66 batches (47%) showed cytopathic changes indicating spontaneous virus contamination of the cultures. Sixty-six strains were isolated and the properties of 23 cytopathic strains were studied. Electron microscopic examinations of 6 virus isolates identified them as members of the herpesvirus group. Serological examinations of 335 clinically normal green monkeys demonstrated antibodies to one of our virus isolates (No. 8532) in 192 (57.3%) animals. Virus strain 8532 was neutralized by antiserum to green monkey cytomegalovirus M-1. The isolates obtained in the present study exhibit the properties previously unknown for green monkey cytomegalovirus such as the capacity for replication in rabbit, sheep, cow, and piglet cell cultures.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000302:isolation & purification / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000382:microbiology*; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000382:microbiology*; D008297:Male; D009500:Neutralization Tests; D011817:Rabbits; D012756:Sheep; D013552:Swine
Izuchenie spontannoĭ tsitomegalovirusnoĭ infektsii kul'tur kletok pochek zelenykh martyshek.