A method for selecting the coding DNA for a protein of known sequence. II. Use of obligatory restriction sites.
A method for selecting the coding DNA of a protein of known sequence in a library of chimeric plasmids constructed with cDNA is described. It is based on the prediction of obligatory restriction sites within the searched cDNA. A partially purified probe can be obtained by taking advantage of the occurrence of two such obligatory sites. Another suggested possibility is to combine one obligatory restriction site and a specific synthetic oligodeoxynucleotide used as primer in classical reverse transcription methods.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006801:Humans; D008722:Methods; D011506:Proteins / Q000235:genetics*
D011506:Proteins; D004247:DNA; D004262:DNA Restriction Enzymes