Low binding of Bolton-Hunter-labelled cholecystokinin-33 to carboxyl-terminal CCK-antibodies.
Labelling of cholecystokinin-33 (CCK33) with [125I]hydroxyphenylpropionic acid-succinimide ester (Bolton-Hunter-reagent) results in labels with excellent immunoreactivity when tested with non-carboxyl-terminal CCK-antibodies but extremely low binding to carboxyl-terminal CCK-antibodies. This finding might explain some of the difficulties experienced with production of immunoreactive CCK33 labels prepared by the Bolton-Hunter method.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002417:Cattle; D002766:Cholecystokinin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D005755:Gastrins / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D011956:Receptors, Cell Surface; D011949:Receptors, Cholecystokinin; D013388:Succinimides; D013552:Swine
D000906:Antibodies; D005755:Gastrins; D011956:Receptors, Cell Surface; D011949:Receptors, Cholecystokinin; D013388:Succinimides; C022581:Bolton-Hunter reagent; D002766:Cholecystokinin