Insertion of new genetic information into bone marrow cells of mice: comparison of two selectable genes.
A system for insertion of new genetic information into mouse hematopoietic cells is described. Two selectable genes were examined: herpesvirus thymidine kinase and a mutant mouse dihydrofolate reductase. The DHFR system appears to be superior in terms of the frequency and stability of gene insertion and expression in hematopoietic tissues. About 70% of mice had indirect (karyotypic) evidence of gene insertion; of these, about 60% (three of five) had stable expression of the inserted mutant DHFR. In contrast, only 13% of mice demonstrated stable karyotypic transformation by HSVtk, and of those with stable transformation five of seven showed persistent viral gene sequences in hematopoietic tissues.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure*; D016026:Bone Marrow Transplantation; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D066298:In Vitro Techniques; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D010641:Phenotype; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000201:enzymology; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics* / Q000494:pharmacology; D014170:Transformation, Genetic
D013762:Tetrahydrofolate Dehydrogenase; D013937:Thymidine Kinase; D008727:Methotrexate