Initiation of replication at specific origins in DNA molecules microinjected into unfertilized eggs of the frog Xenopus laevis.
Initiation of DNA replication at specific origins was observed by electron microscopy after microinjection of pXlr11, pXlr14 or Col E1 plasmid DNA molecules into unfertilized eggs of the frog, Xenopus laevis. These results are in apparent contradiction with published reports (Harland and Laskey, Cell 21, 761-771, 1980; Laskey and Harland, Cell 24, 283-284, 1981) that specific origin sites were not used in Xenopus laevis eggs. We suggest that eucaryotic origins exist that both increase the probability of replication of contiguous sequences and determine the site at which replication is most likely to begin.
D000818:Animals; D003086:Bacteriocin Plasmids; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D005260:Female; D008845:Microinjections; D008854:Microscopy, Electron; D010063:Ovum / Q000378:metabolism*; D010957:Plasmids; D014982:Xenopus laevis
D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes