Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate. III. Subcellular distribution and interaction of 99Tc-DMS complex with macromolecules in rat kidney homogenates in vitro.
Rat kidney tissue homogenates, incubated in vitro with 99Tc-DMS were subjected to differential centrifugation. Specific radioactivities of subcellular fractions were as follows: cytosol 13,552, microsomes 3,600, mitochondria, 2,707 cpm/mg protein, and nuclei, 2,642 cpm/mg DNA. The cytosol fraction was further analyzed by ion-exchange chromatography on DEAE-Sephadex A-25 column. In this way it was separated into several distinct protein zones, three of them bearing a significant amount of 99Tc-DMS preparation. The highest specific radioactivities of proteins were eluted from the DEAE-Sephadex column with 30 mmol/l and 50-110 mmol/l NaCl solution. Total nucleic acids isolated from purified nuclei, mitochondria and microsomes contained a certain amount of bound 99Tc-DMS complex. Nuclear nucleic acids had the highest specific radioactivity, suggesting that 99Tc-DMS complex penetrates into the nuclei.
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000032:analysis; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000032:analysis; D066298:In Vitro Techniques; D007668:Kidney / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008297:Male; D008861:Microsomes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D004113:Succimer; D013438:Sulfhydryl Compounds; D013667:Technetium; D019783:Technetium Tc 99m Dimercaptosuccinic Acid
D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D013667:Technetium; D004113:Succimer