title: Isolation and characterization of the mitochondrial DNA from the florida spiny lobster, Panulirus argus.
abstract: 1. Mitochondria and mitochondrial DNA were isolated from the digestive gland of Panulirus argus. 2. The mitochondrial DNA has an average contour length of 5.13 microns which corresponds to a molecular weight of 10.10 X 10(6) daltons. 3. The molecular weight based on agarose gel electrophoresis of restricted individual DNA samples ranges from 10.04 to 10.4 X 10(6) daltons. 4. Restriction endonuclease analysis with Bam H1 and Eco R1 demonstrate variation in nucleotide sequence between individual lobsters.
mesh_terms: D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D008121:Nephropidae / Q000032:analysis*
chemical_list: D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI