Evaluation in swine of a subunit vaccine against pseudorabies.
A subunit vaccine against pseudorabies virus (PRV) was prepared by treating a mixture of pelleted virions and infected cells with the nonionic detergent Nonidet P-40 and emulsifying the extracted proteins incomplete Freund's adjuvant. Three 7-week-old pigs without antibodies against PRV were given 2 IM doses of this vaccine 3 weeks apart. Thirty days after the 2nd vaccination, 10(6) median tissue culture infective doses (TCID50) of a virulent strain of PRV were administered intranasally. Tonsillar and nasal swabs were collected daily between 2 and 10 days after challenge exposure. The pigs vaccinated with the subunit vaccine were not found to shed virulent PRV. Two groups of five 7-week-old pigs vaccinated with commercially available vaccines, either live-modified or inactivated virus, and subsequently exposed to 10(6) TCID50 of virulent PRV, shed virulent virus for up to 8 days. The subunit vaccine induced significantly higher virus-neutralizing antibody titers than either the live-modified or inactivated virus vaccine.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D009500:Neutralization Tests; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines
D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014764:Viral Proteins; D014765:Viral Vaccines