Binding of carcinoembryonic antigen (CEA) to an anti-CEA immunosorbent in the presence of detergents.
The binding of [125I]CEA to an anti-CEA Sepharose-4B immunosorbent has been investigated in the presence of detergents. Non-ionic detergents (Triton-X-100, Lubrol PX and Tørgitol NPX) at concentrations of 5-40 milligrams did not inhibit the binding of [125I]CEA to the immunosorbent. Sodium deoxycholate decreased the binding from 98% (concentration of detergent 0.6 milligram) to 41% (concentration of detergent 20 milligrams). Sodium dodecyl sulfate inhibited the binding of [125I]CEA to the immunosorbent considerably, even at low concentrations.
D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D003902:Detergents / Q000494:pharmacology*; D006801:Humans; D007164:Immunosorbents; D007457:Iodine Radioisotopes; D017137:Nonoxynol; D017830:Octoxynol; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D002272:Carcinoembryonic Antigen; D003902:Detergents; D007164:Immunosorbents; D007457:Iodine Radioisotopes; D013501:Surface-Active Agents; D000077423:Polidocanol; D017137:Nonoxynol; D011092:Polyethylene Glycols; D017830:Octoxynol