title: Amino-terminal sequence of the Tn3 transposase protein.
abstract: The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D009713:Nucleotidyltransferases / Q000235:genetics; D014176:Protein Biosynthesis; D019895:Transposases
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D009713:Nucleotidyltransferases; D019895:Transposases