Aging of the erythrocyte XIII. Decrease in nitroxide reducing capacity.
A method is proposed for the determination of nitroxide-reducing capacity (NRC) of erythrocytes, and other cells, based on measurements of reduction of a nitroxide stable free radical in cell suspensions by ESR spectroscopy. Comparison of different age fractions of bovine erythrocytes by this method demonstrates a decrease in NRC during cell aging in vivo.
D000818:Animals; D002417:Cattle; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism*; D010084:Oxidation-Reduction; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; C001803:tempol