title: Studies on the toxicity and binding kinetics of abrin in normal and Epstein-Barr virus-transformed lymphocyte culture-I. Experimental results - 4.
abstract: The effects of treatment with varying doses of abrin, a D-galactose binding lectin, on DNA and protein synthesis of normal and Epstein-Barr virus (EBV)-transformed lymphocytes have been previously investigated. Using data on EBV-transformed lymphocyte cell density as a function of both time and dose of abrin, the authors introduced the concept of self- and cross-coupling metabolic variables as a means of understanding how abrin affected DNA and protein uptake. In this paper, the self-coupling constant is studied in more detail and the relationship between DNA and protein synthesis is further expanded. We find that there is a significant linear relationship between DNA and protein synthesis in normal lymphocyte culture as measured by abrin interaction in the culture. We further find that there is a much stronger relationship between these variables in EBV-transformed lymphocyte culture. This relationship is further examined, and possible analytic equations are expressed.
mesh_terms: D000036:Abrin / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002472:Cell Transformation, Viral; D004261:DNA Replication / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000235:genetics*; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D010940:Plant Proteins / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D000036:Abrin