title: Studies on the chalcone synthase gene of two higher plants: petroselinum hortense and matthiola incana.
abstract: Two higher plant systems are presented which allow to study coordinated gene expression of the light-induced metabolic pathway of flavonoid biosynthesis: tissue culture cells of Petroselinum hortense (Apiaceae) and different developmental stages of various genotypes of Matthiola incana (Brassicaceae). The gene structure of the chalcone synthase is mainly studied. A cDNA clone (pLF56) of parsley has been constructed and characterized conferring the chalcone synthase gene sequence. Strong cross hybridization between the parsley cDNA and Matthiola DNA allowed to identify a HindIII fragment (6000 bp) identical in size for parsley and different Matthiola wild type lines and a mutant line.
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000872:Anthocyanins / Q000096:biosynthesis*; D003001:Cloning, Molecular; D046508:Culture Techniques; D004247:DNA; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D008027:Light; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics* / Q000528:radiation effects; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D012333:RNA, Messenger; D004247:DNA; D000217:Acyltransferases; C020141:flavanone synthetase; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII