Modulation of dexamethasone receptor expression in embryonal carcinoma cells and their differentiated derivatives.
The dexamethasone binding capacity of embryonal carcinoma cells and their differentiated derivatives was investigated. Manipulation of the embryonal carcinoma cell-culture conditions resulted in an unstable reversible expression of the glucocorticoid receptors. Stable expression of the receptors is observed when these cells are induced to differentiate. Cells grown under identical conditions were assayed for their ability to bind epidermal growth factor.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D003907:Dexamethasone / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D051379:Mice; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism* / Q000473:pathology; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D003470:Culture Media; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; C028697:dexamethasone receptor; D004815:Epidermal Growth Factor; D003907:Dexamethasone