[Lambda H-lambda T 80 hybrid study of the DNA structural gene region in lambda and phi 80 phages].
Hybrids lambda H lambda T80 are formed due to recombination of the phage lambda att80 and phi 80 prophage partially deleted in the region of structural genes. Genetic structure of 22 independently isolated lambda H lambda T80 hybrids was determined by the restriction method and it was shown that recombination took place in the genes A, C, D and H. The frequencies of hybrid formation diminish from 1.10(-3) to 4.10(-5) for this gene order, which suggests that the polar divergence of nucleotide sequencies in the region of structural genes exists. It was found that formation of hybrids with recombination in the region of "weak" homology (gene H) was possible only when the region of "strong" homology was present in the deleted phi 80 prophage to initiate recombination.
D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics; D003090:Coliphages / Q000187:drug effects / Q000235:genetics*; D003433:Crosses, Genetic; D003434:Crossing Over, Genetic / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes / Q000187:drug effects; D005814:Genes, Viral / Q000187:drug effects; D011995:Recombination, Genetic / Q000187:drug effects
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Izuchenie oblasti strukturneykh genov DNK fagov lambda i fi 80 s pomoshch'iu gibridov lambdaH-lambdaT80.