[An ELISA test for detection of maedi-visna antibodies. Comparative study with gel immunodiffusion and complement-fixation test].
An indirect microELISA test was performed for detection of maedi-visna antibodies in ovine and caprine species. The antigen consisted in viral particles, highly purified by successive ultracentrifugations. By comparative testing of 934 sera in ELISA and gel immunodiffusion, we found a good correlation between these two tests, and moreover, ELISA revealed another 11.3% of positive samples. The precocity of this ELISA was shown by experimental infection of sheep with different strains of maedi-visna: positive sera were detected 7 weeks post-infection, instead 4-5 months with gel immunodiffusion. The complement fixation test was compared with gel immunodiffusion and was found the less sensitive. This ELISA test appeared to be satisfactory, and may be used for early diagnosis of maedi-visna infection.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats / Q000276:immunology; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D012756:Sheep / Q000276:immunology; D014790:Visna-maedi virus / Q000276:immunology*
D000914:Antibodies, Viral
Une technique ELISA pour la détection des anticorps anti-virus maedi-visna. Etude comparative avec l'immunodiffusion en gelose et la fixation du complement.