title: Some effects of copper deficiency on leucocyte function in sheep and cattle.
abstract: Peripheral blood leucocytes from copper-deficient (less than 8 mumol per litre plasma) and copper-sufficient (more than 8 mumol per litre) ewes and lambs were tested for their ability to ingest and kill Candida albicans in vitro. Killing capacity was significantly reduced in both copper-deficient ewes and lambs, although phagocytic capacity was apparently unimpaired. Similar results were obtained with copper-deficient calves, in which it was further shown that copper repletion restored candidacidal activity. Leucocyte and erythrocyte superoxide dismutase (LSOD and ESOD respectively) activities were both significantly decreased in hypocupraemic ewes and there was a linear relationship between their activities in the two cell types: LSOD activities were reduced by approximately 35 per cent and ESOD activities by 67 per cent. Cyanide inhibition tests indicated that the manganese-dependent form of the enzyme contributed little to the total activity of LSOD or ESOD. There was a tendency for leucocytes from copper-deficient ewes to release greater amounts of superoxide anion after stimulation with opsonised zymosan. Their viability was similar to that of leucocytes from control sheep and was unaffected for up to three hours after stimulation.
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D002470:Cell Survival; D003300:Copper / Q000097:blood / Q000172:deficiency*; D004912:Erythrocytes / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology / Q000502:physiology*; D010587:Phagocytosis; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood; D013481:Superoxides / Q000097:blood
chemical_list: D013481:Superoxides; D003300:Copper; D013482:Superoxide Dismutase