Establishment and characterization of an Epstein-Barr virus-negative lymphoma B-cell from a patient with a diffuse large cell lymphoma.
Cell line (LNPL) was established from a tumor biopsy of a nasopharyngeal "histiocytic" lymphoma composed of large noncleaved B-cells. LNPL expressed immunoglobulin M kappa surface immunoglobulin, as did the original tumor. LNPL also expressed Ia antigen but lacked Epstein-Barr virus nuclear antigen. It exhibited a translocation between chromosomes 8 and 14 which has been described in certain other malignant lymphoproliferative disorders. An autologous T-cell line lacked the translocation between chromosomes 8 and 14. LNPL is being used as an immunogen to produce monoclonal antibodies in our laboratory. Such cell lines may facilitate identification of lymphoma-associated surface antigens.
D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology*; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D008224:Lymphoma, Follicular / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D008297:Male
D000956:Antigens, Viral