[The biological action of dithiocarbamates. Changes in serum leucine aminopeptidase induced in vitro by sodium diethyldithiocarbamate].
The Authors demonstrate that serum LAP activity in vitro is inhibited by NaDDTC. This may be related to the NaDDTC chelating-like action on the metallo-enzyme. The trend of the phenomenon follows an exponential pattern. The enzyme activity was completely restored after removal of NaDDTC from the medium by dialysis. The NaDDTC concentration able to inhibit the enzymatic activity in vitro was much higher than in vivo, in experimental animals. The Authors conclude pointing out the problem of a probable effect caused by repeated doses of dithiocarbamates more on enzyme synthesis than on metallic apoenzyme.
D000818:Animals; D003956:Dialysis; D004050:Ditiocarb / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007931:Leucyl Aminopeptidase / Q000037:antagonists & inhibitors / Q000097:blood*; D008297:Male; D011817:Rabbits; D013859:Thiocarbamates / Q000494:pharmacology*
D013859:Thiocarbamates; D004050:Ditiocarb; D007931:Leucyl Aminopeptidase
Azione biologica dei ditiocarbamati. Variazioni di attività di leucinoaminopeptidasi sierica (LAP) indotte "in vitro" da sodiodietilditiocarbamato (NaDDTC).