Sensitivity of proliferating human breast epithelial cells to hypotonic treatment.
An assay for colony-forming cells of breast epithelia derived from normal and malignant surgical specimens is described using an IMR 90 fibroblast feeder layer. Their radiosensitivity (DO: 120-172) is consistent with the proliferative origin of the colonies. Distilled water inhibits proliferation of a proportion of the colony-forming cells after a 1-minute exposure. Continued detection of colonies after 10 minutes of exposure indicates that it is an inefficient way of completely eradicating proliferating epithelial cells of normal and malignant origin.
D001943:Breast Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000473:pathology* / Q000532:radiotherapy; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004848:Epithelium; D005260:Female; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology*
D007038:Hypotonic Solutions