title: Spontaneous rat nephroblastoma: ultrastructure of a transplant line.
abstract: Tumor tissue derived from a nephroblastoma arising spontaneously in an Nb hooded rat and carried for 56 generations as a subcutaneous syngeneic transplant was examined by electron microscopy. Histologically, the transplant showed the typical pattern of dense clusters or sheets of basophilic epithelioid cells set within ramifying tracts of fibrous mesenchyme. At the ultrastructural level, the tumor cells within dense clusters were arranged in anastomotic cords that circumscribed clear intervening spaces. The tumor cells were a monomorphic population possessing the morphologic characteristics of blast cells, including polyribosomes as the predominant cytoplasmic organelle. Furthermore, the close contiguity of cells within the cords, their polyhedral form, and their connection by intercellular junctions established the tumor cell type as epithelial. Consistent with benign stroma, mesenchymal tracts contained highly differentiated, mature fibroblasts, collagen, phagocytes, cells of the lymphoid series, and normal blood vessels. No transitional forms between epithelium and mesenchyme suggestive of bipotential differentiation were seen. Although the data represent only a single transplantation line, the observations support the contention (based on light microscopic appraisal of a number of primary tumors) that nephroblastoma in te rat can be a purely epithelial neoplasm.
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure; D007680:Kidney Neoplasms / Q000648:ultrastructure / Q000662:veterinary*; D008297:Male; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains / Q000033:anatomy & histology*; D012376:Rodent Diseases / Q000473:pathology; D009396:Wilms Tumor / Q000648:ultrastructure / Q000662:veterinary*