Restriction enzyme mapping of the DNA of Streptomyces bacteriophage B alpha and its deletion derivatives.
Cleavage analysis of actinophage B alpha DNA was done with several restriction enzymes, and a restriction map of the DNA was determined. The DNA appeared to carry cohesive ends. Deletion mutants of actinophage B alpha were isolated by five cycles of treatment with 15 mM PPi. Both mutants had deletions of 2.5 of 1.8 megadaltons near one end of the genome, and one of them lost the single EcoRI cleavage site.
D001435:Bacteriophages / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D011756:Diphosphates / Q000494:pharmacology; D005814:Genes, Viral; D009154:Mutation; D013302:Streptomyces
D004279:DNA, Viral; D011756:Diphosphates; D004262:DNA Restriction Enzymes