[Isolation of a purified theta-hemolysin from C1. perfingens type A and its use for titrating toxins and sera].
Purified Cl. perfringens theta-hemolysin with a specific activity of 15-16 X 10(5) HU per mg of protein nitrogen, toxic for mice when injected intravenously, has been obtained by precipitation with zinc chloride, gel filtration through Sephadex G-100 and ion-exchange chromatography on DEAE Sephadex A-50. 1 Dcl of the toxin is 0.2-0.4 mg. The yield of theta-hemolysin amounting to 20-25% has been achieved, the degree of its purification reaching 80- to 120-fold. Equine anti-theta hemolytic serum has been obtained. The method for the determination of the anti-theta toxin-binding properties of Cl. perfringens toxins and the quantitative content of anti-theta-hemolysin in sera has been developed.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D003016:Clostridium perfringens / Q000276:immunology; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000276:immunology / Q000302:isolation & purification*; D006736:Horses / Q000276:immunology; D007106:Immune Sera / Q000032:analysis* / Q000302:isolation & purification; D007114:Immunization; D066298:In Vitro Techniques; D051379:Mice; D012756:Sheep / Q000276:immunology
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007106:Immune Sera
Vydelenie ochischennogo téta-gemolizina C1. perfringens tipa A i ispol'zovanie ego dlia titrovaniia toksinov i syvorotok.