[Human basophil degranulation test (HBDT) for diagnosis of respiratory allergic disorders. A study of eighty-four HDBT performed in forty-seven patients (author's transl)].
The HBDT uses basophils of patients with respiratory allergic disorders. In this simple method the basophils are stained by toluidine blue and the erythrocytes are destroyed. The basophils are then exposed to solutions containing different concentrations of the antigen. The basophil degranulation rate in response to a given antigen can be determined. The test is positive if the degranulation rate is over 50% and negative if the rate is under 30%. Our clinical study induces 47 patients. The correlate rate between HBDT and skin tests is 81,5%. This test's technical simplicity allows it's routine use by a non specialized laboratory.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens; D001491:Basophils / Q000276:immunology / Q000648:ultrastructure*; D003594:Cytoplasmic Granules; D005260:Female; D006801:Humans; D007428:Intradermal Tests; D008297:Male; D008875:Middle Aged; D012130:Respiratory Hypersensitivity / Q000175:diagnosis*
D000485:Allergens
Le test de dégranulation des basophiles humains (TDBH). Son application dans le diagnostic des allergies respiratoires. Etude à propos de quatre-vingt-sept TDBH effectués sur quarante-sept patients.