Post mortem changes in drug-metabolizing enzymes of rat liver and human liver.
The aim of this investigation was to obtain information on the time-dependent decrease of the drug-metabolizing system in autolysing rat liver, and also in human cadaver liver. Rat liver, divided into three parts, was tested immediately after removal and 6 and 12 hrs later. Parameters investigated were: microsomal protein, cytochrome P-450, NADPH cytochrome C reductase, glucose-6-phosphatase, aminopyrine-N-demethylation and aniline-p-hydroxylation. In human liver, samples taken from 0.5 up to 3.5 hrs after death, microsomal protein cytochrome P-450, NADPH cytochrome C reductase and phospholipids were tested. Nearly all parameters based on microsomal protein decrease during autolysis, but by different amounts. Interestingly, the cytochrome P-450 content of patients with signs of shock 12 hrs before death is significantly lower than in patients without shock.
D000328:Adult; D000633:Aminopyrine N-Demethylase / Q000032:analysis; D000815:Aniline Hydroxylase / Q000032:analysis; D000818:Animals; D001835:Body Weight; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D005260:Female; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D011180:Postmortem Changes; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D005952:Glucose-6-Phosphatase