Identification and purification of a liver microsomal glucose 6-phosphatase.
1. Hepatic glucose 6-phosphatase activity was purified 65-fold in good yield over that in cholate-solubilized microsomal fractions. 2. This preparation still contained five major polypeptides and numerous minor contaminants. 3. The smallest of the five major polypeptides (Mr approx. 18 500) could be purified from heat-treated microsomal fractions. 4. Antisera raised against the heat-stable protein doublet was used to immunoprecipitate specifically glucose 6-phosphatase activity from cholate-solubilized microsomal fractions. 5. This work indicates that hepatic microsomal glucose 6-phosphatase appears to be one or both of the low-molecular-weight heat-stable polypeptides.
D000818:Animals; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D005952:Glucose-6-Phosphatase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D008862:Microsomes, Liver / Q000201:enzymology*; D011817:Rabbits
D005952:Glucose-6-Phosphatase