Evaluation of solubilized herpes simplex virus membrane antigens in diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA).
Cell membranes were prepared from herpes simplex virus (HSV) type 1-infected cells and solubilized with a low concentration of sodium deoxycholate. The supernatant after ultracentrifugation was used as antigen in a newly developed solid phase assay, diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA). Antigen solubilization was almost complete, all HSV glycoproteins were represented, the yield of antigen in the solubilization process was high, and the presence of a detergent, sodium deoxycholate, did not interfere with the adsorption of antigen to the solid phase. DIG-ELISA was compared with the neutralization test for the determination of HSC antibodies and zone diameters showed a good correlation with the titre obtained by the neutralization test.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000954:Antigens, Surface; D000956:Antigens, Viral; D002460:Cell Line; D006224:Cricetinae; D003840:Deoxycholic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D003840:Deoxycholic Acid