[Kinetic study of serotonin metabolism in the suprachiasmatic nucleus of the rat: neuroendocrine incidence (author's transl)].
The suprachiasmatic nucleus (SCN), which is implicated in the regulation of several cyclic neuroendocrine rhythms, displays a conspicuous and apparently specific serotoninergic innervation. Our study was intended to establish more precise correlations between the incidence of serotonin (5 HT) metabolism in the activity of the SCN, and neuroendocrine rhythms. For this purpose, castrated female rats, having subcutaneous implants of estradiol, were studied. These animals display very marked circadian fluctuations in plasma levels of the pituitary hormones ACTH, LH and PRL; a relatively well-synchronized increment in all these hormones occurs between 11.00 and 15.00. Punches were obtained to determine the endogenous content of 5 HT, measured by a radioenzymatic technique, simultaneously in SCN and median eminence (ME). An index of SCN activity was determined from in vivo SCN 2-deoxy (1-14C) glucose (DG) uptake; the retention was estimated on radioautographs. Endogenous level of 5 HT displayed a marked circadian rhythm with a peak between 12.00 and 15.00 in the SCN; 5 HT levels were constant throughout the day in the ME. 14C-DG uptake was greater at 15.00 than at 9.00. However, after PCPA treatment or raphe lesions, the uptake of 14C-DG was the same at 9.00 and 15.00. Taken together, these results strongly suggest that in our model: (1) SCN displays a rhythm of activity in the light period; (2) SCN displays specific rhythms in the content of 5 HT; (3) the SCN rhythm of activity must be under an inhibitory control of 5 HT, since the lowest metabolic level is increased at 9.00 by inactivation of 5 HT system; (4) the close relationships between the initial release phase of several pituitary hormones, the increase of metabolic activity in the SCN and the higher level of the 5 HT in the nucleus suggest that 5 HT terminals in the SCN play an important part in the control of cyclic hormonal secretion.
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D002940:Circadian Rhythm; D003847:Deoxyglucose / Q000378:metabolism; D007031:Hypothalamus / Q000502:physiology*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000097:blood; D010902:Pituitary Gland / Q000502:physiology*; D011388:Prolactin / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D003847:Deoxyglucose
Etude cinétique du métabolisme de la 5 HT dans le noyau suprachiasmatique de rat. Incidence neuroendocrinienne.