Ultraviolet irradiation inhibits encapsidation of simian virus 40 chromatin.
During normal maturation and majority of pulse-labeled simian virus 40 DNA progresses from chromatin to previrions and virions within 5 h. UV light inhibits this progression. In heavily irradiated cultures (108 J m-2) most of the simian virus 40 DNA synthesized immediately before irradiation remains as chromatin for at least 5 h. This inhibition of maturation seems to be a result of the inhibition of protein synthesis. The data suggest that the pool of proteins required for maturation is sufficient to convert one-third of the simian virus 40 DNA molecules labeled in a 10-min pulse (at 33 h postinfection) from chromatin to previrions and virions and is exhausted within 1 h.
D002213:Capsid / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism* / Q000528:radiation effects; D013539:Simian virus 40 / Q000254:growth & development / Q000378:metabolism*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000096:biosynthesis; D014771:Virion / Q000378:metabolism*
D002843:Chromatin; D004279:DNA, Viral; D014764:Viral Proteins