Synthesis of disaturated phosphatidylcholine by cholinephosphotransferase in rat lung microsomes.
1. Incubation of rat lung microsomes with cytidine diphospho[methyl-14C]choline resulted in synthesis of radioactively labeled phosphatidylcholine. 2. 10-15% of this phosphatidylcholine appeared to be disaturated species. In similar experiments with rat liver microsomes only 2-3% of the radioactivity was present in the disaturated species. 3. When de novo synthesis was blocked after 5 min by addition of Ca2+ no increase in the proportion of disaturated phosphatidylcholine was observed upon further incubation of lung microsomes. Under these conditions the enzymes involved in a remodeling mechanism, i.e. phospholipase A and acyl-CoA: lysophosphatidyl-choline acyltransferase, remain fully active. 4. Addition of diacylglycerols from egg phosphatidylcholine containing trace amounts of di[1-14C]palmitoyl glycerol resulted in direct incorporation of 14C label into phosphatidylcholine. The rate of phosphatidylcholine synthesis measured from incorporation of di[1-14C]palmitoyl glycerol equalled that observed with labeled CDP choline. 5. These results support the conclusion that disaturated phosphatidylcholine in lung can be formed by direct utilization of disaturated diacylglycerol and is not produced exclusively via remodelling of de novo synthesized unsaturated species.
D000818:Animals; D002250:Carbon Radioisotopes; D003566:Cytidine Diphosphate Choline; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism*; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats
D002250:Carbon Radioisotopes; D010713:Phosphatidylcholines; D003566:Cytidine Diphosphate Choline; D010770:Phosphotransferases; D002798:Diacylglycerol Cholinephosphotransferase