title: Fluctuation analysis of Na+ channels modified by batrachotoxin in myelinated nerve.
abstract: (1) Single myelinated nerve fibers of Rana esculenta were treated with the steroidal alkaloid batrachotoxin, and Na+ currents and Na+-current fluctuations were measured near the resting potential under voltage-clamp conditions. Between test pulses the fibres were held at hyperpolarizing membrane potentials. (2) The spectral density of Na+-current fluctuations was fitted by the sum of a 1/f component and a Lorentzian function. The time constant tau c = 1/(2 pi fc) obtained from the corner frequency fc of the Lorentzian function approximately agreed with the activation time constant tau m of the macroscopic currents. (3) The conductance gamma of a single Na+ channel modified by batrachotoxin was calculated from the integral of the Lorentzian function and the steady-state Na+ current. At the resting potential V = 0 we obtained gamma - 1.6 pS, higher gamma-values of 3.2 and 3.45 pS were found at V = --8 and --16 mV, respectively. (4) The conductance of a modified Na+ channel is significantly lower than the values 6.4 to 8.85 pS reported in the literature for normal Na+ channels. Hence, our experiments are in agreement with the view that batrachotoxin acts in an 'all-or-none' manner on Na+ channels and creates a distinct population of modified channels.
mesh_terms: D000818:Animals; D001496:Batrachotoxins / Q000494:pharmacology*; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials; D009413:Nerve Fibers, Myelinated / Q000378:metabolism*; D011893:Rana esculenta; D012964:Sodium / Q000378:metabolism*
chemical_list: D001496:Batrachotoxins; D007473:Ion Channels; D012964:Sodium