The inhibition of bovine and rat parotid deoxyribonuclease I by skeletal muscle actin. A biochemical and immunocytochemical study.
Rat and bovine parotid gland and pancreas contain deoxyribonuclease I (DNAase I) activities in different amounts. The DNAase I activity in tissue homogenates of bovine and rat parotid gland can be inhibited by addition of monomeric actin, as with the enzyme of bovine pancreas. The isolated DNAase I species from bovine and rat parotid gland differ in their molecular weights and also in their affinities for monomeric actin, being lowest for rat parotid DNAase I (5 X 10(6)M(-1). Antibodies raised against rat and bovine parotid and bovine pancreatic DNAase I can be used to study the subcellular localization of DNAase I in these tissues by indirect immunofluorescence. DNAase I was found to be confined solely to the secretory granules of the tissue from which it was isolated.
D000199:Actins / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D003429:Cross Reactions; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009708:Nucleotidases / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010306:Parotid Gland / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology
D000199:Actins; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D009708:Nucleotidases; C030287:nucleotidase; D002918:Chymotrypsin