[Mapping of 2 cloned DNA fragments containing repetitive mouse genome regions, actively transcribing into pre-mRNA].
Sequence organization of two cloned mouse genomic DNA fragments which are efficiently transcribed into pre-mRNA was studied. In both cases, the arrangement of sequences hybridizing to polysomal mRNA (or cDNA), double-stranded RNA (dsRNA-B) isolated from pre-mRNA and oligo (dT), was established. In one fragment, the regions transcribed are represented by three different repetitive mRNA (or cDNA) hybridizing sequences interspersed into the AT-rich tract, the type B-2 sequence and the unique DNA sequence which also transcribed into mRNA. Nuclear pre-mRNA of 28S is hybridized to three repetitive mRNA hybridizing regions. The significance of such a complex array of repeated sequences inside eukaryotic transcription units and their possible role in mRNA processing and splicing is discussed.
D000818:Animals; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics; D004274:DNA, Recombinant; D005796:Genes; D051379:Mice; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000235:genetics*; D011132:Polyribosomes / Q000648:ultrastructure; D012322:RNA Precursors; D012326:RNA Splicing; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes
Kartirovanie dvukh klonirovannyk fragmentov DNK, soderzhashchikh povtoriaiushchiesia i aktivno transkribiruiushchiesia v sostave pro-mRNK uchasti genoma myshi.