Partial characterization of temperature-sensitive mutants of foot-and-mouth disease virus, O1 caseros strain.
The preliminary characterization of four temperature-sensitive (ts) mutants of the O1 Caseros strain of foot-and-mouth disease virus is described. Two mutants, ts 6 and ts 40, showed a very low RNA synthesis rate at nonpermissive temperature and were classified phenotypically as RNA(-). Shift-up experiments demonstrated an incapacity to organize the RNA replicative process at nonpermissive temperature. Another mutant (ts 139) behaved phenotypically as RNA(+) and its virions were more thermolabile than the wild type virus, so the defect in this mutant is likely to be in one of its structural proteins. Finally, mutant ts 5 was phenotypically RNA(+); its leak production at nonpermissive temperature was high, and the shift-up experiment showed a defect that was expressed either late in the replicative cycle or continuously.
D000818:Animals; D005537:Aphthovirus / Q000235:genetics* / Q000254:growth & development; D002478:Cells, Cultured; D006224:Cricetinae; D007668:Kidney; D008647:Mesocricetus; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D014776:Virus Cultivation; D014779:Virus Replication
D012367:RNA, Viral