Generation of leukotriene B4, its all trans isomers and 5-hydroxyeicosatetraenoic acid by rat basophilic leukaemia cells.
1 When rat basophilic leukaemia (RBL-1) cells were incubated for 4 min, in the presence of arachidonic acid (AA) and calcium ionophore A23187, three isomers of leukotriene (LT) B4 and 5-hydroxyeicosatetraenoic acid (5-HETE) were generated. 2 The isomers of LTB4 separated by reverse phase high pressure liquid chromatography (h.p.l.c.) were found to be 5 (s)-dihydroxy-6, 14-trans-8,10-trans-eicosatetraenoic acid: 5(S), 12(R)-dihydroxy-6,14-trans-8,10-trans-eicosatetraenoic acid and 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid or LTB4. 3 5(S), 12(R)-cis, trans, trans-LTB4 produced by RBL-1 cells caused aggregation of rat polymorphonuclear leucocytes (PMNs). Its other two isomers (5(S), 12(S)-all trans-LTB4; 5(S), 12-(R)-all trans-LTB4) were much less active and 5-HETE was found to be inactive. 4 5(S), 12(R)-cis, trans, trans-LTB4 formed from RBL-1 cells contracted guinea-pig parenchymal strips (GPPs) and was indistinguishable from synthetic 5(S), 12(R)-cis, trans, trans-LTB4. The other two isomers of LTB4. i.e. 5(S), 12(S)-all trans-LTB4 and 5(S), 12(R)-all trans-LTB4 as well as 5-HETE were inactive on GPP. 5 RBL-1 LTB4 was equipotent with LTD4. in most experiments, in contracting GPP but had no activity on guinea-pig ileum smooth muscle (GPISM). 6 RBL-1 cells contain the enzyme systems necessary for generating LTB4 as well as the leukotriene(s) with amino acid residues at C-6.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001491:Basophils / Q000378:metabolism; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D006168:Guinea Pigs; D006893:Hydroxyeicosatetraenoic Acids; D066298:In Vitro Techniques; D007942:Leukemia, Experimental / Q000378:metabolism*; D007975:Leukotriene B4; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid