Transposition of cloned P elements into Drosophila germ line chromosomes.
Recombinant DNA carrying the 3-kilobase transposable element was injected into Drosophila embryos of a strain that lacked such elements. Under optimum conditions, half of the surviving embryos showed evidence of P element-induced mutations in a fraction of their progeny. Direct analysis of the DNA of strains derived from such flies showed them to contain from one to five intact 3-kilobase P elements located at a wide variety of chromosomal sites. DNA sequences located outside the P element on the injected DNA were not transferred. Thus P elements can efficiently and selectively transpose from extrachromosomal DNA to the DNA of germ line chromosomes in Drosophila embryos. These observations provide the basis for efficient DNA-mediated gene transfer in Drosophila.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D006824:Hybridization, Genetic; D008297:Male; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D004251:DNA Transposable Elements