title: The stereochemical course of phosphoryl transfer catalysed by glucose 6-phosphatase.
abstract: Rat liver microsomal glucose 6-phosphatase catalyses phosphoryl transfer between D-glucose 6-[(R)-16O,17O,18O]phosphate and D-glucose with retention of configuration at the phosphorus atom. Since individual phosphoryl-transfer steps appear in general to occur with inversion of configuration, this observation is most simply interpreted in terms of a double-displacement mechanism with a phosphoryl-enzyme intermediate. Such an intermediate has been proposed previously from kinetic and 32P-labelling experiments.
mesh_terms: D000818:Animals; D005947:Glucose / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008862:Microsomes, Liver / Q000201:enzymology; D008968:Molecular Conformation; D010103:Oxygen Isotopes; D010766:Phosphorylation; D051381:Rats
chemical_list: D005958:Glucosephosphates; D010103:Oxygen Isotopes; D019298:Glucose-6-Phosphate; D005952:Glucose-6-Phosphatase; D005947:Glucose