Amphotericin B and the elasmobranch rectal gland: implications for the relationship between oxygen consumption and ion transport.
In slices of the dogfish rectal gland, amphotericin B produced increases in oxygen consumption and ouabain binding similar to those produced by cyclic AMP, except that the increases induced by the latter were inhibited by furosemide whereas those resulting from the addition of amphotericin B were unaffected by the diuretic. Unlike cyclic AMP, however, amphotericin B failed to stimulate secretion by the isolated perfused gland. These results support the suggestion that the increases in ouabain binding and oxygen consumption produced by cyclic AMP result simply from an increased sodium entry into the cells, and as such can be mimicked by amphotericin B, whereas secretion rate itself depends on some additional, cyclic AMP-induced process. The implications of this for determinations of the relationship between rates of oxygen consumption and ion transport are discussed.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D004284:Dogfish / Q000378:metabolism*; D005665:Furosemide; D010042:Ouabain / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D012491:Salt Gland / Q000378:metabolism*; D012754:Sharks / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology
D010042:Ouabain; D005665:Furosemide; D000666:Amphotericin B; D012964:Sodium; D013806:Theophylline; D000242:Cyclic AMP