HPLC analysis of erythrocyte pyrimidine-5'-nucleotidase inhibition by lead.
The activity of pyrimidine-5'-nucleotidase (P5N) (EC 3.1.3.5) was assayed in microsamples of rat blood using high performance liquid chromotography (HPLC). The assay is based on the measurement of enzymatically formed uridine in erythrocyte hemolysates (10-50 microliter) and produces a linear activity curve from 5 to 1000 microM/g Hb/h. Acute (22 h) administration of lead acetate ip to rats induced a dose-dependent inhibition of P5N activity in erythrocyte samples.
D015720:5'-Nucleotidase; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D066298:In Vitro Techniques; D007854:Lead / Q000494:pharmacology*; D008297:Male; D009708:Nucleotidases / Q000037:antagonists & inhibitors / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D007854:Lead; D009708:Nucleotidases; D015720:5'-Nucleotidase