The effect of incubation temperature on the propagation of duck adenovirus (virus 127-like) in duck and chicken cells.
Duck adenovirus (Cornell strain) was propagated in duck and chicken embryo cells at 37.5 C and at 40 C. In duck cells, virus levels, as indicated by HA titers, peaked earlier at 40 C than at 37.5 C. High titers were eventually observed in duck cells at both temperatures. In chicken embryo fibroblasts, no titers were observed at 37.5 C, whereas low titers were observed at 40 C. Evidence of virus propagation was not detected in chicken embryo liver and kidney cells.
D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000259:Aviadenovirus / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004372:Ducks; D004625:Embryo, Nonmammalian; D005347:Fibroblasts; D006386:Hemagglutination Tests / Q000662:veterinary; D007668:Kidney; D008099:Liver; D013696:Temperature; D014776:Virus Cultivation; D014779:Virus Replication
D000914:Antibodies, Viral