Induction of ornithine decarboxylase by treatment of guinea pig lymphocytes with phospholipase C.
Treatment of guinea pig lymphocytes with Clostridium perfringens phospholipase C but not with Naja naja snake venom phospholipase A2 increased ornithine decarboxylase activity. The increase in ornithine decarboxylase activity was suppressed by actinomycin D or cycloheximide, suggesting that de novo syntheses of RNA and protein are necessary for the increase in the enzyme activity. These results suggest that the activation of phospholipase C rather than that of phospholipase A2 is responsible for induction of ornithine decarboxylase during lymphocyte transformation.
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D002262:Carboxy-Lyases / Q000235:genetics*; D002478:Cells, Cultured; D003016:Clostridium perfringens / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004546:Elapid Venoms; D004790:Enzyme Induction; D006168:Guinea Pigs; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000235:genetics*; D010740:Phospholipases / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D010738:Type C Phospholipases / Q000494:pharmacology*
D004546:Elapid Venoms; D003609:Dactinomycin; D003513:Cycloheximide; D010740:Phospholipases; D010738:Type C Phospholipases; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase