Modulation of microsome-mediated benzo[a]pyrene-metabolism by serum.
The effects of serum on the metabolism of benzo[a]pyrene (BP) by liver microsomes from 3-methylcholanthrene-treated rats were studied. In the presence of serum, the aryl hydrocarbon hydroxylase activities were enhanced to 160-570% of the control value. Enhancement of BP-metabolism by serum was also revealed by high performance liquid chromatography, but the relative amounts of a series of BP-metabolites were not changed appreciably by addition of serum. Binding of BP-metabolites to DNA was influenced by serum: adducts derived from two stereo-isomeric BP-7,8-di-hydrodiol-9,10-oxides were increased, while those from 9-hydroxybenzo[a]pyrene-4,5-oxide were reduced.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D001790:Blood Physiological Phenomena; D002273:Carcinogens / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D001580:Benzopyrenes; D002273:Carcinogens; D001564:Benzo(a)pyrene; D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases