Evidence for non-chromosomal hepatitis B virus surface (HBsAg)- and core antigen (HBcAg)-specific DNA sequences in a hepatoma cell line.
As demonstrated previously, a "beta particle" fraction isolated from the cytoplasm of PLC/PRF/5 cells contains hepatitis B virus (HBV)-specific DNA. Here, further evidence is provided that the specificity of the DNA for HBV is represented at least by sequences coding for the surface and core antigen (HBsAg and HBcAg). This was shown by two different hybridization techniques. One of them, the technique of Southern, distinguished these hybrid molecules formed from those containing HBV DNA integrated into chromosomes. The HBV-specific beta particle DNA forms two distinct bands separate from the high molecular weight cellular DNA.
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002875:Chromosomes; D004279:DNA, Viral; D005814:Genes, Viral; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens