Effects of carcinogen and/or mutagen on normal and gonatotropin-primed ovaries of mice.
The influence of pregnant mare serum gonadotropin (PSGM) on the induction of ovarian tumors by carcinogen(DMBA) and/or mutagens (EMS and BMS) has been studied in Swiss albino mice. Priming of the ovaries of 6-week-old mice with PMSG (50 IU/mouse) 24 h before intragastric intubation of DMBA (5 mg/mouse) resulted in a significant increase in ovarian tumors when harvested 6 months after the treatment. A similar experiment done on 8-week-old mice yielded confirmatory results. Mutagens (EMS and BMS) failed, at given dose levels (200 mg/kg and 300 mg/kg body weight), to induce tumors in normal as well as PMSG-primed ovaries. Pretreatment of animals with PMSG-enhanced DMBA-induced oocyte depletion and also increased the DMBA-3H activity in the ovaries. The augmentation of DMBA-induced tumors in PMSG-primed ovaries could be due to increased uptake of DMBA and increased depletion of oocytes. It is also possible that PMSG-primed ovaries provided more proliferating granulosa cells (and macromolecules) for the interaction of DMBA (or its metabolites) and thereby increased the chances of cell transformation in the ovaries.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D005020:Ethyl Methanesulfonate / Q000378:metabolism / Q000633:toxicity; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008698:Mesylates / Q000633:toxicity; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009865:Oocytes / Q000187:drug effects; D010051:Ovarian Neoplasms / Q000139:chemically induced*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism
D001551:Benz(a)Anthracenes; D006064:Gonadotropins, Equine; D008698:Mesylates; D009153:Mutagens; C018011:butyl methanesulfonate; D015127:9,10-Dimethyl-1,2-benzanthracene; D005020:Ethyl Methanesulfonate