Immunization of mouse spleen cell cultures in the absence of serum and its proteins using SiO2 and 2-mercaptoethanol.
High recoveries of plaque-forming cells were obtained in a serum-free medium using 2-mercaptoethanol and SiO2. Under such conditions an increase in the number of plaque-forming cells occurred between the fourth and fifth day of incubation, as compared with cultures with foetal calf serum. The addition of glutathione to the medium had a stimulating effect, it was however not absolutely necessary for a positive response. The recovery of plaque-forming cells was strongly influenced by an interdependence of cell density and amount of SiO2 gel particles.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006462:Hemolytic Plaque Technique; D008623:Mercaptoethanol / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012822:Silicon Dioxide / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D013997:Time Factors
D003470:Culture Media; D008623:Mercaptoethanol; D012822:Silicon Dioxide