Organization and stability of endogenous xenotropic murine leukemia virus proviral DNA in mouse genomes.
In a series of blot hybridization experiments, using a xenotropic envelope probe and restriction enzymes known to cut xenotropic proviral DNA a single time (EcoRI) or not at all (HindIII), we have studied the organization and relationship of endogenous xenotropic env-related sequences in various mouse strains. Multiple copies (18 to 28) of xenotropic env-reactive fragments were found in all mouse DNAs after digestion with either HindIII or EcoRI, and the majority of fragments were of sizes compatible with their origin from full-length proviral DNA. Five HindIII and five EcoRI restriction fragments were common to all inbred mouse DNAs tested. In addition, each strain exhibited unique characteristic xenotropic env-reactive bands; these bands were remarkably stable during many years of inbreeding. The cleavage patterns characteristic of each strain were also useful for showing genealogical relatedness among the various inbred mice.
D000818:Animals; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII