The conformation of adenovirus VAI-RNA in solution.
The secondary structure of an adenovirus associated low molecular weight RNA (VAI-RNA) has been studied by partial digestion with T1-RNase and S1-endonuclease followed by T1-fingerprint analysis. The empirical secondary structure has been compared with two computer generated models based on minimal free energy of the structure. The results suggest that VAI-RNA in solution has a compact structure with a free energy of around -60 kcal with two stems and four bulge regions. The implication of this structure for the function of VAI-RNA is discussed.
D000256:Adenoviridae / Q000235:genetics*; D001483:Base Sequence; D003201:Computers; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000378:metabolism; D006163:Ribonuclease T1 / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases
D012367:RNA, Viral; D004720:Endonucleases; D006163:Ribonuclease T1; D015719:Single-Strand Specific DNA and RNA Endonucleases