Activities of enzymes involved in purine metabolism and some related adenine nucleotide concentrations of leucocytes in renal failure.
We have studied purine metabolism in mononuclear and polymorphonuclear cells from uraemic patients using microradiochemical enzyme assays and high-pressure liquid chromatography. In mononuclear cell lysates the mean activities of adenosine deaminase (EC 3.5.4.4) and 5'-nucleotidase (EC 3.1.3.5) were significantly diminished. The activities of adenylate kinase (EC 2.7.4.3), purine nucleoside phosphorylase (EC 2.4.2.1), adenine phosphoribosyltransferase (EC 2.4.2.7), and hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) were not significantly different in the two groups. The activities of adenosine deaminase and adenine phosphoribosyltransferase were reduced in the polymorphonuclear cell lysates. No clear differences emerged in the concentration of adenine nucleotides in the mononuclear cells. The significance of these changes, which are less marked than those in erythrocytes, is discussed with reference to the immunodeficiency associated with uraemia.
D000227:Adenine Nucleotides / Q000097:blood*; D000228:Adenine Phosphoribosyltransferase / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood; D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000263:Adenylate Kinase / Q000097:blood; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000097:blood; D007676:Kidney Failure, Chronic / Q000201:enzymology*; D007962:Leukocytes / Q000201:enzymology*; D009708:Nucleotidases / Q000097:blood; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood; D011687:Purines / Q000097:blood*; D014511:Uremia / Q000201:enzymology
D000227:Adenine Nucleotides; D011687:Purines; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D011683:Purine-Nucleoside Phosphorylase; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D000263:Adenylate Kinase; D009708:Nucleotidases; D000243:Adenosine Deaminase