title: Penicillinase-producing Neisseria gonorrhoeae: a molecular comparison of 5.3-kb and 7.4-kb beta-lactamase plasmids.
abstract: Restriction endonuclease analysis and heteroduplex studies indicate that the only difference between the 5.3-kilobase (kb) and 7.4-kb plasmids from beta-lactamase-producing Neisseria gonorrhoeae is that the latter is the 5.3-kb plasmid with a 2.1-kb insertion. The insertion is bounded by inverted repeats of approximately 300 base pairs. Several plasmids from beta-lactamase-producing N. gonorrhoeae isolated at different times and in different countries were compared. Nine 5.3-kb plasmids were examined and found to be indistinguishable, as were sixteen 7.4-kb plasmids.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes; D010405:Penicillinase / Q000096:biosynthesis / Q000235:genetics*; D011815:R Factors; D012091:Repetitive Sequences, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes; D010405:Penicillinase; D001618:beta-Lactamases