[Isoenzymatic forms and distribution of adenylate kinase and creatine kinase in the bovine adrenal medulla].
Adenylate kinase, creatine kinase as well as their substrate and product levels have been investigated in the adrenal medullary tissue. The concentration of adenine nucleotides and creatine + creatine phosphate are 12.6 +/- 0.4 and 6.9 +/- 0.4 mumol/g wet weight respectively. Adenylate kinase is mainly in the cytosol; only 4% was found in mitochondria. The cytosol enzyme presents a Km for AMP of 5 X 10(-4) M and a Ki for diadenosine pentaphosphate of 0.6 X 10(-6) M. In gel electrophoresis, only one band of adenylate kinase activity can be seen, and its mobility is different from that of the brain enzyme. Creatine kinase from adrenal medulla is mainly found in cytosol; only 3-4% was associated with mitochondria. The cytosolic enzyme is mainly the BB isozyme form.
D000263:Adenylate Kinase / Q000302:isolation & purification*; D000313:Adrenal Medulla / Q000201:enzymology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002837:Chromaffin Granules / Q000201:enzymology; D003402:Creatine Kinase / Q000302:isolation & purification*; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification*; D008928:Mitochondria / Q000201:enzymology; D010770:Phosphotransferases / Q000302:isolation & purification*
D007527:Isoenzymes; D010770:Phosphotransferases; D003402:Creatine Kinase; D000263:Adenylate Kinase
Formas isoenzimáticas y distribución de adenilato kinasa y creatín kinasa en médula suprarrenal bovina.