Molecular biology of rotaviruses. IV. Molecular cloning of the bovine rotavirus genome.
A new cloning strategy has been developed for cloning the genomes of double-stranded RNA viruses by using bovine rotavirus as a test system. The major modification adopted was the use of denatured polyadenylated double-stranded RNA as the template for reverse transcriptase. This allowed the two complementary strands of cDNA to be synthesized in a single reaction and removed the need for S1 nuclease digestion to remove the 5' hairpin structure normally generated in cDNA synthesis.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000235:genetics*; D012401:Rotavirus / Q000235:genetics*
D004274:DNA, Recombinant; D012330:RNA, Double-Stranded; D004247:DNA; D004262:DNA Restriction Enzymes