Neurotensin receptors in the rat bronchi.
The isolated, circular preparations of the left and right bronchi of the rat were examined for mechanical responses to neurotensin (NT) and other vasoactive peptides. NT caused concentration-dependent increases in the isometric tension of the unstimulated preparations, with an apparent affinity higher than for the cholinergic agonists but with considerably lower intrinsic activity. Pronounced tachyphylaxis to NT was observed. NT potentiated the atropine-sensitive increase in tension resulting from electrical field stimulation. Neither atropine nor methysergide abolished the response to NT in the unstimulated preparations. Bradykinin and to a lesser extent angiotensin II contracted the unstimulated preparations and both systemic peptides enhanced the cholinergic output in response to field stimulation. Substance P and VIP on the other hand were without effects in the stimulated and unstimulated bronchi. The results are consistent with the presence of receptors for NT on the presynaptic cholinergic terminals as well as on the post-synaptic smooth muscles of the rat bronchi.
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D001980:Bronchi / Q000032:analysis*; D002217:Carbachol / Q000494:pharmacology; D005260:Female; D008784:Methysergide / Q000494:pharmacology; D009130:Muscle, Smooth / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis*; D018028:Receptors, Neurotensin; D012701:Serotonin / Q000494:pharmacology; D013997:Time Factors
D011956:Receptors, Cell Surface; D018028:Receptors, Neurotensin; D000804:Angiotensin II; D012701:Serotonin; D002217:Carbachol; D001920:Bradykinin; D008784:Methysergide