[Detection of T-2 toxin in grains using thin layer chromatography with silica gel (TLC)].
The TLC method of the detection of T-2 toxin in grains is described. A thin layer of silica gel with a sensitivity of 3 mg/kg is used in this method. In comparison with the biological assay for dermal irritability, the TLC method is 30 times less sensitive. However, it has one great advantage over the bioassay: its specificity. It is recommended for practice to use a screening method based on the test for the dermal irritability of rabbits. When the content of toxins is higher, the analytic method can be used for trying to identify the given mycotoxin of the trichothecene group. T-2 toxin was detected in none of the 33 samples of tested grains (14 samples of barley, 7 wheat, 5 rye, 5 oats, 2 samples of maize).
D002855:Chromatography, Thin Layer / Q000379:methods; D002523:Edible Grain / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D012822:Silicon Dioxide; D013605:T-2 Toxin / Q000032:analysis*
D012717:Sesquiterpenes; D012822:Silicon Dioxide; D013605:T-2 Toxin
Prúkaz T-2 toxinu v zrninách chromatografií na tenké vrstvĕ silikagelu (TLC).