Monoclonal antibody for identification of human duct cell carcinoma of pancreas.
Monoclonal antibody to duct cell surface marker (HP-DU-1), produced by hybridoma, was shown to be specific to duct cell surface antigen when tested against normal adult and 12-20-week-old fetal human pancreases. There were no common HLA antigens for locus A, B, C and DR among the 10 pancreas donors; nor did HP-DU-1 reveal any affinity for lymphocytes of donors whose pancreases were used as antigen for immunization of BALB/c mice. Seven out of 7 human pancreas adenocarcinomas including a poorly differentiated human pancreatic tumor expressed surface determinants detectable by HP-DU-1. Cell lines CAPAN-1 and CAPAN-2, derived from human pancreatic adenocarcinoma, also revealed the same antigenic determinants.
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D010179:Pancreas / Q000276:immunology / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006680:HLA Antigens