Cloning and characterization of cDNA copies of the 7S RNAs of HeLa cells.
We have cloned cDNA copies of in vitro adenylated 7S RNA of HeLa cells. The most representative clones in the library contain DNA fragments copied from the 7SL and 7SK small RNAs. The two classes of recombinants share no homology. The 7SL RNA contains at the 5' end of the molecule sequences homologous to the Alu sequence family. Hybridization to human genomic DNA shows that the 7SL and 7SK clones are homologous to two different families of repetitive sequences.
D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D012334:RNA, Neoplasm; D004247:DNA; D004262:DNA Restriction Enzymes