Hormone-sensitive adenylate cyclase in glomerular cells: possible role for inflammatory diseases of the glomerulus.
Using the adenylate cyclase assay after Ross we examined hormone sensitivity of isolated glomerular cells. cAMP production was increased 1.3--1.6-fold by stimulation with isoproterenol, 1.5--1.8 times by prostaglandin E1 and 1.4--1.5 times by histamine. The isoproterenol reaction could be completely inhibited by propranolol, the histamine effect was abolished by the H2-blocking agent cimetidine. As control we applied sodium fluoride, which directly activates the catalytic adenylate cyclase unit, increasing the activity 1.8--2.7 times (depending on the method of homogenization). These findings could reflect some physiological or pathophysiological implications, which are discussed in the present report.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D005921:Glomerulonephritis / Q000201:enzymology*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007678:Kidney Glomerulus / Q000187:drug effects* / Q000201:enzymology; D011458:Prostaglandins E / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats
D006633:Histamine Antagonists; D011458:Prostaglandins E; D006632:Histamine; D006160:Guanosine Triphosphate; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D007545:Isoproterenol