Is transformation associated with an increased error frequency in mammalian cells?
Acute starvation of mammalian cells for amino acids results in translational errors that may be detected by two-dimensional polyacrylamide gel electrophoresis. Using this as an assay for error frequency in mammalian cells, we investigated the hypothesis that neoplastic transformation was associated with an increased error frequency which in turn leads to an increased mutation rate and a decreased efficiency of regulatory controls (phenomena of tumor progression). Although we found that transformation was not always associated with an increased level of mistranslation we showed that SV40 transformation increased the level of translational errors in all cell types tested.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000494:pharmacology*; D006801:Humans; D008715:Methionine / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000302:isolation & purification; D013539:Simian virus 40 / Q000235:genetics
D000596:Amino Acids; D011506:Proteins; D006639:Histidine; D008715:Methionine