Characterization of tumor-specific transplantation antigen induced in cells infected or transformed by polyomavirus.
Tumor-specific transplantation antigen (TSTA) activity was investigated (with the use of inbred BALB/c and C3H mice and inbred Fisher and Wistar rats) in various early polyomavirus (Py)-coded proteins by three methodologies: 1) immunoprecipitation by anti-T-antigen sera, gel slicing, and immunization; 2) affinity chromatography with the use of anti-tumor-associated antigen serum; and 3) use of a Py mutant, NG18. The results obtained by all three techniques allowed refutation of the hypothesis that the three major species of T-antigen possess the main TSTA activity, particularly middle-sized T-antigen which is a plasma membrane protein. TSTA activity was found to be associated with proteins having a molecular weight of approximately 37,000. The exact origin of this protein is discussed.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D009154:Mutation; D011120:Polyomavirus; D051381:Rats; D011919:Rats, Inbred Strains
D000951:Antigens, Neoplasm