Further studies of naturally occurring latent bovine herpesvirus infection.
Attempts were made to enhance the isolation rate of latent bovine herpesviruses from trigeminal ganglia by using a fibroblastic fetal bovine kidney cell line, in addition to Madin-Darby bovine kidney cells, and by superinfecting with a temperature-sensitive helper strain of bovine herpesvirus-1 (BHV-1). Four isolates of latent BHV-1 were obtained from 44 pairs of trigeminal ganglia--thus reflecting no increase in isolation rate over that previously observed. Two isolates of a latent bovine herpesvirus-3 strain DN599 were also obtained. Analysis of plaque morphology of BHV-1 isolates obtained in this and a previous study indicated that several biologically heterogeneous strains were capable of establishing latent infections.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006564:Herpesviridae / Q000254:growth & development / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D013696:Temperature; D014276:Trigeminal Nerve / Q000382:microbiology; D010948:Viral Plaque Assay