Chemical modifications of pokeweed antiviral protein: effects upon ribosome inactivation, antiviral activity and cytotoxicity.
Pokeweed antiviral protein (PAP) is a protein known to inactivate eukaryotic ribosomes by an unknown enzymatic action and inhibit the production of mammalian viruses in tissue culture. This protein was subjected to a variety of chemical modifications to determine their effects upon ribosomal inactivation, antiviral action, and cytotoxicity. It was found that modifications of a number of different amino acid residues had similar effects upon all 3 activities. Also the inactivation of PAP with diethylpyrocarbonate was not due to its reaction with a histidine residue but to a modification of an unidentified amino acid residue.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D009699:N-Glycosyl Hydrolases; D010649:Phenylalanine / Q000378:metabolism; D010940:Plant Proteins; D014176:Protein Biosynthesis; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*; D018139:Simplexvirus / Q000187:drug effects*
D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D010649:Phenylalanine; D001120:Arginine; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein