Modification and exploitation of a poliovirus-induced membrane complex by superinfecting ME virus.
The replication of Mouse Elberfeld (ME) virus was accelerated when HEp-2 cells were mixedly infected with poliovirus in the presence of guanidine. The latent period of the replication of ME virus was shortened by 3 h when cells were preinfected for at least 2 h with poliovirus and inhibited by guanidine. Simultaneous infection with poliovirus and ME virus resulted in a shortening by 1 h of the latent period of ME virus replication. The accelerated replication of ME virus was shown to be due to modification and exploitation of a membrane complex induced by poliovirus in the presence of guanidine; on superinfection ME virus successively modified this poliovirus-induced complex of 470S ("light' complex) into a "heavy' complex of 700S specific for ME virus.
D002460:Cell Line; D004770:Enterovirus / Q000254:growth & development*; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000502:physiology* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000382:microbiology* / Q000648:ultrastructure; D017955:Poliovirus / Q000502:physiology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D006146:Guanidines; D019791:Guanidine