Modulation of adenosine 3', 5'-cyclic monophosphate-dependent protein kinase in skin.
Incubation in vitro of full-thickness guinea-pig skin with epinephrine or histamine resulted in the formation of adenosine 3', 5'-cyclic monophosphate (cyclic AMP) and increase in the cyclic AMP-dependent protein kinase activity ratio. The effect was dose-dependent between 10(-5) and 10(-3) M concentration of the agents. Histamine antagonists, diphenhydramine (H1) and cimetidine (H2) prevented stimulation by histamine in a dose-dependent manner suggesting the presence of both types of receptors in full-thickness skin. Cyclic AMP accumulation with a concomitant increase in protein kinase activity denotes that phosphoproteins may have an important role in skin biology.
D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D004155:Diphenhydramine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D006633:Histamine Antagonists; D066298:In Vitro Techniques; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D012867:Skin / Q000187:drug effects*
D006633:Histamine Antagonists; D002927:Cimetidine; D006632:Histamine; D004155:Diphenhydramine; D000242:Cyclic AMP; D011494:Protein Kinases; D004837:Epinephrine