Physical mapping of human adenovirus type 7 DNA using a simple and sensitive method of terminal labeling.
The tyrosine-containing peptide covalently attached to each 5'-terminus of adenovirus type 7 (Ad 7; Greider) DNA was labeled with 125I. The 5'-labeled DNA was subjected to digestion with several restriction endonucleases and the size of the labeled terminal fragments was determined. Partial hydrolysis by these endonucleases generated a series of labeled fragments which were fused to the terminal fragments and could, therefore, be detected by autoradiography. From the sizes of the partial products the location of the cleavage sites of the enzymes on Ad7 DNA could be determined. The subgenomic DNA extracted from incomplete particles by protease treatment could also be labeled with 125I, since it was found to contain the tyrosine-containing peptide covalently attached to the preferentially packaged left end of the genome.
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D002277:Carcinoma; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D007457:Iodine Radioisotopes; D009062:Mouth Neoplasms; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D011485:Protein Binding; D014443:Tyrosine
D004279:DNA, Viral; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D014443:Tyrosine; D004262:DNA Restriction Enzymes; D010447:Peptide Hydrolases