Acylation: a new post-translational modification specific for plasma membrane-associated simian virus 40 large T-antigen.
SV40 transformed mouse cells (mKSA) were labeled in parallel with either [35S]methionine or [3H]palmitate and subfractionated. Nuclear extracts and solubilized plasma membranes were analyzed for the presence of either 35S- or 3H-labeled SV40 large tumor antigen by immunoprecipitation and SDS polyacrylamide gel electrophoresis. The majority of the [35S]methionine labeled large T was recovered from the nuclear fraction, only minor amounts were detected in plasma membranes. In contrast, large T labeled specifically with [3H]palmitate was found only in the plasma membrane fraction. Our results demonstrate a specific acylation of large T associated with plasma membranes, suggesting that the membrane location of this predominantly nuclear protein is specific.
D000215:Acylation; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000302:isolation & purification; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002472:Cell Transformation, Viral; D051379:Mice; D008807:Mice, Inbred BALB C; D011499:Protein Processing, Post-Translational; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor