[Effect of formaldehyde on the ribonucleoprotein structure of Sendai virus].
The effect of formaldehyde on the conformation of Sendai virus ribonucleoprotein (RNP) was studied by electron microscopy and a spectrophotometric method. The effect of formaldehyde on RNP conformation was found to depend strongly on the ionic strength of the solution. Under conditions of a low ionic strength in the presence of 1.5% formaldehyde the tightly coiled rods of RNP stained with uranyl acetate become loosely coiled or almost completely extended. At the same time, formaldehyde reaction with RNP results in a decrease in extinction of the RNP suspension in the 250 to 290 spectral region. However, RNP incubation with formaldehyde in the presence of 0.5M NaCl caused no changes in the morphology and spectral properties of the RNP under study. The results indicate that formaldehyde cannot be used for fixation of Sendai virus nucleocapsids before negative staining for electron microscopy examinations.
D005557:Formaldehyde / Q000494:pharmacology*; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000032:analysis*; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects* / Q000648:ultrastructure; D010455:Peptides / Q000032:analysis; D011487:Protein Conformation / Q000187:drug effects; D012261:Ribonucleoproteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014764:Viral Proteins / Q000032:analysis*
D009698:Nucleoproteins; D010455:Peptides; D012261:Ribonucleoproteins; D014764:Viral Proteins; D005557:Formaldehyde
Issledovanie vliianiia formal'degida na strukturu ribonukleoproteida virusa Sendaĭ.