title: Recombination during gene transfer into mouse cells can restore the function of deleted genes.
abstract: Two plasmids containing nonoverlapping deletions of the herpes simplex virus thymidine kinase gene were introduced into thymidine kinase-deficient mouse L cells by DNA-mediated gene transfer. Thymidine kinase-producing transformants were generated by a mixture of the two plasmids at a frequency significantly greater than that generated by either plasmid alone. Southern blot analyses demonstrated that functional thymidine kinase genes were generated by homologous recombination between the two deletion mutants.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002872:Chromosome Deletion; D005818:Genetic Engineering; D051379:Mice; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D018139:Simplexvirus; D013937:Thymidine Kinase / Q000235:genetics*
chemical_list: D013937:Thymidine Kinase