Synthesis and properties of human beta-endorphin-(1-17) and its analogs.
Four analogs of human beta-endorphin (beta h-EP) were synthesized by the solid-phase method: beta h-EP-(1-17) (I), [D-Ala2]beta h-EP-(1-17) (II), [Gln8]-beta h-EP-(1-17) (III) and [D-Ala2, Gln8]-beta h-EP-(1-17) (IV). Measurement in a radio-receptor binding assay with use of tritiated beta h-EP as primary ligand gave relative potencies as follows: Met-enkephalin, 100; I, 33; II, 47; III, 889; IV, 123; beta h-endorphin, 2253.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004590:Electrophoresis, Paper; D004723:Endorphins / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D010446:Peptide Fragments / Q000138:chemical synthesis*; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism; D013329:Structure-Activity Relationship
D000596:Amino Acids; D004723:Endorphins; D007202:Indicators and Reagents; D010446:Peptide Fragments; D011957:Receptors, Opioid