title: Contrasting patterns of protein kinase activities among normal peripheral blood lymphocyte subpopulations and lymphoid cell lines.
abstract: Human peripheral mononuclear cell suspensions were separated into E-rosette-positive (T cell) and E-rosette-negative (non-T cell) fractions and assayed for cAMP binding activity and histone and casein kinase activity, in the presence and absence of cAMP. Each of these activities was higher in E-rosette-positive than E-rosette-negative fractions. Cyclic AMP binding and histone kinase activities were further noted to be higher in T lymphoblastoid cell lines when compared to lines of non-T lymphoid cell origin. In addition. DEAE-cellulose chromatography revealed a qualitative contrast of isoenzymic composition of protein kinase from these cell lines. Cyclic AMP-independent casein kinase activity was high in both types of cell lines. These results suggest that high protein kinase activity is characteristic of T cells and may be related to their higher sensitivity to cAMP agonists in comparison to non-T cells. This study also provides further evidence for an association between increased cAMP-independent protein kinase activity and the transformed state, which has been noted for a variety of other cell types.
mesh_terms: D001665:Binding Sites; D047388:Casein Kinases; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000145:classification / Q000201:enzymology*; D008297:Male; D006656:Protamine Kinase / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology
chemical_list: D000242:Cyclic AMP; D011494:Protein Kinases; D047388:Casein Kinases; D006656:Protamine Kinase