Inhibition by lithium of PGE1-sensitive adenylate cyclase in neuroblastoma X glioma hybrid cells: approach to the attenuation of the opiate withdrawal syndrome.
Exposure of neuroblastoma X glioma hybrid cells to LiCl (Li+) for 48 h significantly reduces prostaglandin E1 (PGE1)-dependent cyclic adenosine monophosphate (AMP) accumulation. When applied simultaneously with morphine for the same period, Li+ inhibits the excessive accumulation of cyclic AMP in response to PGE1 characteristically exhibited by these cells upon opiate withdrawal. Likewise, Li+ inhibits, in vitro, PGE1-dependent cyclic AMP accumulation in tissue slices from rat corpus striatum. Prophylactic administration of Li+ in the diet attenuates the withdrawal symptoms in morphine-addicted rats. These findings support clinical reports that suggest that prophylactic treatment with Li+ attenuates the severity of the opiate withdrawal syndrome.
D000067956:Adenylyl Cyclase Inhibitors; D000527:Alprostadil; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002478:Cells, Cultured; D003342:Corpus Striatum; D000242:Cyclic AMP / Q000378:metabolism; D005910:Glioma / Q000201:enzymology; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D009374:Neoplasms, Experimental / Q000201:enzymology; D009447:Neuroblastoma / Q000201:enzymology; D009293:Opioid-Related Disorders / Q000201:enzymology*; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy*
D000067956:Adenylyl Cyclase Inhibitors; D011458:Prostaglandins E; D008094:Lithium; D000242:Cyclic AMP; D000527:Alprostadil