Influence of salts on electrostatic interactions between poliovirus and membrane filters.
Neither solutions of salts nor solutions of detergents or of an alcohol at pH 4 are capable of eluting poliovirus adsorbed to membrane filters. However, solutions containing both a salt, such as magnesium chloride or sodium chloride, and a detergent or alcohol at pH 4 were capable of eluting adsorbed virus. The ability of ions to promote elution of virus at low pH in the presence of detergent or alcohol was dependent on the size of the ions and the ionic strength of the medium. These results suggest that both electrostatic and hydrophobic interactions are important in maintaining virus adsorption to membrane filters. Hydrophobic interactions can be disrupted by detergents or alcohols. It appears that electrostatic interactions can be disrupted by raising the pH of a solution or by adding certain salts. Disruption of either electrostatic or hydrophobic interactions alone does not permit efficient elution of the adsorbed virus at low pHs. However, when both interactions are disrupted, most of the poliovirus adsorbed to membrane filters is eluted, even at pH 4.
D000327:Adsorption; D000440:Butanols / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004563:Electrochemistry; D005374:Filtration / Q000295:instrumentation; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D017955:Poliovirus / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology*; D020002:tert-Butyl Alcohol
D000440:Butanols; D003902:Detergents; D012965:Sodium Chloride; D020002:tert-Butyl Alcohol