Identification and characterization of Epstein-Barr virus-related surface polypeptides expressed on n-butyrate-treated P3HR-1 cells.
Seven polypeptides [300,000 (300K), 250K, 150K, 140K, 120K, 87K and 52K] were identified as Epstein-Barr virus(EBV)-related surface polypeptides expressed on n-butyrate-treated P3HR-1 cells. Five polypeptides (300K, 250K, 150K, 120K and 87K) were sensitive to phosphonoacetic acid, and four of these (300K, 250K, 120K and 87K) were glycoproteins. The 300K and 250K polypeptides had a slightly higher molecular size and the 87K polypeptide a slightly lower molecular size than the corresponding polypeptides detected in virus-producing P3HR-1 cells and in the envelope of EBV particles.
D000956:Antigens, Viral / Q000032:analysis*; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000032:analysis / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D002087:Butyrates; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006023:Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D020148:Butyric Acid