Formation of leukotrienes E3, E4 and E5 in rat basophilic leukemia cells.
Rat basophilic leukemia (RBL-1) cells incubated with ionophore A23187 and 5,8,11-eicosatrienoic acid produced three slow-reacting substances identified as leukotrienes C3, D3 and E3 by spectroscopic, chromatographic and enzymatic methods. 5,8,11,14,17-Eicosapentaenoic acid was similarly converted by RBL-1 cells to leukotrienes C5, D5. and E5. Leukotrienes C4, D4 and E4 were also formed in these experiments from endogenous arachidonic acid. Time-course studies, incubations with 3H-labeled leukotriene C3 and effects of acivicin [L-(alpha S, 5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid; a gamma-glutamyl transpeptidase inhibitor] indicated that leukotrienes C and D are intermediates in the formation of leukotrienes E. L-Cysteine enhanced the conversion of leukotriene C3 to leukotriene D3 and inhibited further degradation of leukotriene D3 to leukotriene E3.
D015126:8,11,14-Eicosatrienoic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood* / Q000378:metabolism; D001491:Basophils / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D007555:Isoxazoles / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000378:metabolism*; D017999:Leukotriene E4; D051381:Rats; D013189:SRS-A / Q000097:blood*
D001095:Arachidonic Acids; D007555:Isoxazoles; D013189:SRS-A; D016718:Arachidonic Acid; D000001:Calcimycin; D017999:Leukotriene E4; D015126:8,11,14-Eicosatrienoic Acid; C010944:mead acid; D003545:Cysteine; C006303:acivicin; D005998:Glycine