Chromosome localization of highly repetitive human DNA's and amplified ribosomal DNA with restriction enzymes.
Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the informative C-band patterns had recognition sequences that were four or five, but not six, base pairs long and did not contain a cytosine-guanine doublet. In both rat and human chromosomes, regions containing amplified ribosomal RNA genes were specifically removed by the restriction endonuclease Msp I.
D002871:Chromosome Banding; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005784:Gene Amplification; D005796:Genes; D006801:Humans; D012335:RNA, Ribosomal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes