Biochemical analyses of EDTA extracts and collagenase digests from bone and skin of Wistar rats.
Using EDTA extraction and collagenase digestion, rat bone and rat skin were compared in terms of their content of hydroxyproline, hexoses, uronic aicd, sialic acid and plasma proteins. The collagen content of the organic matrix from both tissues was similar. Greater differences were observed in the sialic acid and uronic acid content of the matrix, bone containing higher amounts; smaller differences were found in the levels of hexoses, albumin, IgG and alpha 1 acid-glycoprotein, which are higher in EDTA extracts from bone. The DEAE-cellulose chromatography of the EDTA extracts and soluble collagenase digests indicated the presence of a variety of glycoproteins and a proteoglycan fraction. An acidic glycoprotein, corresponding to sialoprotein, was present in bone but not in skin extracts.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D001842:Bone and Bones / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D003094:Collagen / Q000032:analysis; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D003012:Microbial Collagenase / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis; D012867:Skin / Q000032:analysis*; D014574:Uronic Acids / Q000032:analysis
D001798:Blood Proteins; D002241:Carbohydrates; D012794:Sialic Acids; D014574:Uronic Acids; D003094:Collagen; D004492:Edetic Acid; D003012:Microbial Collagenase