Choline and PAH transport across blood-CSF barriers: the effect of lithium.
The components of the blood-CSF barrier responsible for the transport of p-aminohippuric acid (PAH) and choline from CSF to blood were identified using in vitro preparations of frog choroid plexus and arachnoid membranes. Choline was transported out of CSF across the arachnoid, while PAH was transported out across the choroid plexus. Probenecid and ouabain blocked both processes. The effect of Li on these transport processes was tested by the addition of 5 mM LiCl to the incubation media. Li increased, by a factor of two, choline transport across the arachnoid, but there was no effect of Li on PAH transport across the plexus. Lithium was passively transported across the choroid plexus, and we suggest that the major transport pathway is through the tight junctions. The steady-state distribution of Li between the choroidal epithelium and the incubation medium was only half that expected for passive distribution. This suggests the existence of sodium/lithium countertransport in these epithelial cell membranes.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001099:Arachnoid / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002712:Chlorides / Q000378:metabolism / Q000494:pharmacology*; D002794:Choline / Q000378:metabolism*; D002831:Choroid Plexus / Q000187:drug effects; D008094:Lithium / Q000378:metabolism / Q000494:pharmacology*; D018021:Lithium Chloride; D010042:Ouabain / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011892:Rana catesbeiana; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D002712:Chlorides; D010042:Ouabain; D008094:Lithium; D018021:Lithium Chloride; D002794:Choline; D011339:Probenecid; D010130:p-Aminohippuric Acid