Detection of serum albumin receptor in hepatitis B virus carriers by enzyme-linked immunosorbent assay.
The relationship between glutaraldehyde-treated polymeryzed human serum albumin (pHSA) and HBe antigen (HBeAg)-positive serum was examined by the use of a new enzyme-linked immunosorbent assay (ELISA). The author succeeded in measuring the pHSA binding activity (pHSA-BA) of HB surface antigen (HBsAg) particles in the present ELISA method using horseradish peroxidase-labelled pHSA after fixation of HBsAg on an anti-HBs-coated well of polystyrene microplates. In HBeAg-positive group, the pHSA-BA of sera of 40 asymptomatic carriers and 2 chronic persistent hepatitis (CPH) patients were higher than those of 8 chronic active hepatitis (CAH) (p less than 0.01) and 8 liver cirrhosis sera (p less than 0.05). On the contrary, in the anti-HBe-positive group the pHSA-BA of 17 asymptomatic carriers and 3 CPH sera were lower than those of 8 CAH (p less than 0.005) and 10 liver cirrhosis patient sera (p less than 0.005). In the both-negative group the pHSA-BA of 8 asymptomatic carrier and 3 CPH sera were also lower than that of 8 CAH (p less than 0.05). In acute exacerbation of HBsAg-positive CAH the pHSA-BA elevated one to two months before the peak of S-GPT level, being correlated with the DNA-polymerase activity. Because of its apparent reproducibility, it is concluded that low cost and some advantages may have clinical utility in the same setting as the HBeAg is now used.
D002353:Carrier State / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000097:blood* / Q000276:immunology; D006511:Hepatitis B Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006801:Humans; D008103:Liver Cirrhosis / Q000276:immunology; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis*
D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface