Human serum albumin labelled with 99Tcm and 131I for total blood volume determination: A critical and comparative analysis in vivo and in vitro.
The increased plasma volume or total blood volume determined with radioactive tracers often have no physical or pathologic explanation. The plasma volume and blood dilution were simultaneously measured in 5 patients who had received human serum albumin labelled with 99Tcm or 131I. In each case and for each tracer, a biexponential dilution curve was obtained. The measured plasma volume was consistently higher after 99Tcm-labelled albumin. The results are correlated with the presence of non-protein artifacts arising from the labelling process and proteinaceous compounds arising from denaturation.
D001811:Blood Volume Determination / Q000379:methods*; D002850:Chromatography, Gel; D005260:Female; D006207:Half-Life; D006349:Heart Valve Diseases / Q000097:blood* / Q000601:surgery; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D008954:Models, Biological; D011865:Radioisotope Dilution Technique; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis; D013667:Technetium
D007457:Iodine Radioisotopes; D011933:Reagent Kits, Diagnostic; D012709:Serum Albumin; D013667:Technetium