Comparison of an ELISA technique with quantal micro-neutralization test for serotyping of HSV-1 or HSV-2-infected patients.
An enzyme-linked immunosorbent assay (ELISA) using semi-purified herpes simplex antigens extracted from cell nuclei was employed for typing of 32 sera collected from patients infected with HSV-1 or HSV-2. The results were in agreement with those obtained by the quantal micro-neutralization test. Moreover, sera with antibodies which could not be detected by micro-neutralization test could be typed by the ELISA technique according to the HSV isolates.
D000914:Antibodies, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000382:microbiology*; D006801:Humans; D009500:Neutralization Tests; D012703:Serotyping; D018139:Simplexvirus / Q000145:classification* / Q000276:immunology
D000914:Antibodies, Viral