Effects of vasopressin and urea on Ca2+-calmodulin-dependent renal prostaglandin E.
Vasopressin (AVP stimulated immunoreactive E (iPGE) synthesis and the release of [3H]arachidonate (AA) from prelabeled slices of rat inner medulla (IM) in the presence but not in the absence of Ca2+ (plus 2 mM EGTA). Urea (700-1,200 mosM) inhibited these actions. By contrast Ca2+ deprivation or urea did not suppress AVP-induced increases in cAMP or stimulation of iPGE by exogenous AA. At 10-50 microM, trifluoperazine (TFP) suppressed AVP-induced increases in [3H]AA release and iPGE accumulation, but not increases in cAMP. Basal acyl hydrolase activity (AH) of 2,000 g particulate fractions of IM was suppressed by EGTA. Ca2+, but not Mg2+ or AVP, restored AH to levels observed in the absence of EGTA. Ca2+-induced increases in particulate AH were inhibited by urea (700-1,200 mosM) or TFP (10-50 microM), but not 1,000 mosM NaCl. Partial depletion of particulate calmodulin-like activity suppressed Ca2+-induced increases in AH. The latter was restored with 1 microM purified exogenous calmodulin but not troponin C. The results demonstrate that AVP stimulation of AA and PGE release in IM are Ca2+-dependent processes suppressed by urea. They also suggest a role for Ca2+-calmodulin-dependent AH in the control of PG synthesis in IM.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002147:Calmodulin / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis*; D014268:Trifluoperazine / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology*
D001095:Arachidonic Acids; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D011458:Prostaglandins E; D001127:Arginine Vasopressin; D014268:Trifluoperazine; D016718:Arachidonic Acid; D014508:Urea; D000242:Cyclic AMP; D010740:Phospholipases; D002118:Calcium