Comparative results of respiratory virus isolation attempts in chorioallantoic membrane fragments and embryonated chicken eggs.
Attempts performed with 39 samples of nasopharyngeal secretion resulted in the isolation of 21 (54%) hemagglutinating viral agents in chorioallantoic membrane (CAM) fragments, while a single strain (2.5%) was isolated in the embryonated egg. The time interval required for virus isolation in CAM fragments ranged from 4 to 20-24 days and was longer then 10 days in the embryonated egg. Maintenance of CAM fragments in roller tubes leads to a more abundant virus multiplication and, implicitly, to a reduction of the time interval necessary for virus isolation.
D000482:Allantois; D000818:Animals; D002642:Chick Embryo; D006386:Hemagglutination Tests; D006801:Humans; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D012140:Respiratory Tract Diseases / Q000382:microbiology*; D018108:Respirovirus / Q000302:isolation & purification*; D014777:Virus Diseases / Q000382:microbiology*