Sensitive detection of specific repair endonucleases: radial diffusion assay utilizing differential alkaline denaturation of supercoiled and nicked PM2 DNA in agarose gels.
D004260:DNA Repair; D004278:DNA, Superhelical; D004279:DNA, Viral; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043224:Deoxyribonuclease (Pyrimidine Dimer); D003850:Deoxyribonuclease I; D043223:Deoxyribonuclease IV (Phage T4-Induced); D003851:Deoxyribonucleases / Q000032:analysis* / Q000378:metabolism; D004058:Diffusion; D004720:Endonucleases / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008837:Micrococcus / Q000201:enzymology; D009691:Nucleic Acid Denaturation; D012685:Sepharose
D004278:DNA, Superhelical; D004279:DNA, Viral; D012685:Sepharose; D003851:Deoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043224:Deoxyribonuclease (Pyrimidine Dimer); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase