Abortive infection of neural cells by herpes simplex virus type 2.
The nature of the refractoriness of C6 rat glioma cells to herpes simplex virus type 2 (HSV-2) was examined. Infection of C6 cells with HSV-2 results in low virus yields, not exceeding the input virus. Although virus growth studies suggested a restricted cycle of virus replication, synthesis of HSV-2 DNA and HSV-2-specific antigens could not be detected. In addition, HSV-2 yields in C6 cells were unaffected by interferon, cycloheximide, tunicamycin, actinomycin D and cytosine arabinoside. However, trypsin, but not EDTA, treatment of infected C6 cells at 4 hours postinfection (p.i.) reduced maximal HSV-2 yields at 24 hours p.i. by 61 percent. These data: 1) indicate that HSV-2 fails to replicate in C6 cells and is prohibited from directing the synthesis of virus macromolecules; and 2) suggests that the increment of HSV-2 yields observed during the synthesis phase of the virus growth cycle represents re-envelopment and egress of a portion of the input virus.
D000818:Animals; D000956:Antigens, Viral; D000963:Antimetabolites / Q000494:pharmacology; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D005910:Glioma / Q000382:microbiology; D006561:Herpes Simplex / Q000382:microbiology*; D009374:Neoplasms, Experimental / Q000382:microbiology; D009457:Neuroglia / Q000382:microbiology*; D051381:Rats; D018139:Simplexvirus / Q000276:immunology / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D000963:Antimetabolites; D004279:DNA, Viral; D014357:Trypsin