Serological differentiation of foot-and-mouth disease virus on electron microscope grids coated with protein A and antibody.
A serological technique using electron microscope grids coated with protein A and antiserum was able to detect foot-and- mouth disease virus particles in oesophageal-pharyngeal fluids from infected cattle without the need for prior concentration of the sample. The technique was adapted to differentiate serologically among foot-and-mouth disease virus types A, O and C with antigen-adsorbed sera. When grids were coated with heterotypic antigenadsorbed antisera, the homotypic antigen could be observed in viral specimens containing 10(5) PFU/mL, but the heterotypic antigen was not visualized until its concentration was about tenfold higher. Grids coated with the appropriate antigen-adsorbed antiserum can thus be used to indicate foot-and-mouth disease viral serotypes in specimens containing less than 10(6) PFU/mL.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D005537:Aphthovirus / Q000145:classification* / Q000276:immunology / Q000648:ultrastructure; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D005536:Foot-and-Mouth Disease / Q000382:microbiology; D007106:Immune Sera / Q000276:immunology; D008854:Microscopy, Electron; D013205:Staphylococcal Protein A / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D013205:Staphylococcal Protein A