Determination of bacmecillinam, an amdinocillin prodrug, in human and canine whole blood by reversed-phase liquid chromatography.
Bacmecillinam is an amdinocillin prodrug designed to be easily hydrolyzed in biological materials, so special procedures were developed for the collection of blood specimens. Whole blood was collected in tubes containing bacampicillin as an adsorption inhibitor and kept at -70 degrees; the extracting solvent, hexane-methylene chloride (9:1, v/v), was added to the cold tubes, and the extraction was performed during the thawing of the samples. The organic phase was partially evaporated before a reextraction to a small volume of acidic aqueous phase was made. The separation was performed on a microparticulate. C18-alkyl bonded silica packed in glass-lined stainless steel columns. Mobile phase was a buffer (pH 6)-acetonitrile mixture containing N-hexyl-N-methylamine as an adsorption inhibitor. The detection limit was 600 pg/ml of whole blood, and the within-run precision (srel%) was approximately 8% at the 5-ng/ml level.
D000560:Amdinocillin / Q000031:analogs & derivatives / Q000097:blood*; D000818:Animals; D001646:Bile / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010397:Penicillanic Acid / Q000097:blood*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D010397:Penicillanic Acid; C021145:bacmecillinam; D000560:Amdinocillin