Band 1 and voltage-sensitive Na+ channels are not codistributed in cultured rodent neuronal cells.
The relationship of the mouse nervous system specific band 1 protein to the putative high molecular weight component of the Na+ channel was investigated using antibody to band 1. Morphologic differentiation of cultured neuroblastoma cells has been reported to increase the quantity of the putative Na+ channel high molecular weight component. Morphologically differentiated clone NB2a neuroblastoma cells have 2-3 times the amount of band 1 and 1.5 times the relative rate of synthesis of band 1 as undifferentiated cells. The anti-band 1 serum reacts with both adult mouse and rat brain but not 3 cultured rat neuronal lines known to have active Na+ channels. Thus either band 1 is not a component of the Na+ channel or individual cultured murine neuronal lines has distinct macromolecular Na+ channels.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D011232:Chemical Precipitation; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D007473:Ion Channels / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000032:analysis / Q000276:immunology; D009423:Nervous System Neoplasms / Q000032:analysis / Q000276:immunology; D009447:Neuroblastoma / Q000032:analysis / Q000276:immunology; D009474:Neurons / Q000032:analysis* / Q000166:cytology / Q000276:immunology
D006023:Glycoproteins; D007473:Ion Channels