Removal of benzo[a]pyrene from cells by various components of medium.
Benzo[a]pyrene is removed from cells in culture by various additions to the medium. During post-treatment incubation, WI-38 fibroblasts were incubated with a low density, very low density and high density lipoproteins, delipidated or complete serum or plasma, or serum albumin. The time course of removal was followed. Increasing concentrations of lipoproteins resulted in increasing percentages of removal of benzo[a]pyrene from cell membranes. The most efficient addition was 10% complete human plasma. These results indicate that benzo[a]pyrene remains at or close to the plasma membrane for at least several hours and readily redistributes to medium components.
D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008168:Lung / Q000196:embryology
D001580:Benzopyrenes; D003470:Culture Media; D008077:Lipoproteins, LDL; D001564:Benzo(a)pyrene