Effect of pulsed ultrasound on human erythrocytes in vitro.
Pulsed echo sonication of whole blood affects the O2-Hb dissociation curve and produces modifications of the erythrocyte membrane, whereas Hb remain unchanged. After sonication ATP-ase (Na+K+ dependent) activity and agglutination disappear. Sonication detaches erythrocytes-membrane antigens which are found in the supernatant, this effect is reversible. AB antigens detached by sonication were transferred to sonicated O group erythrocytes. Examination by transmission electron microscopy suggested that the glycoproteic cup or glycocalyx of the red cells was removed as a result of sonication.
D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000502:physiology* / Q000648:ultrastructure; D006386:Hemagglutination Tests; D006801:Humans; D010108:Oxyhemoglobins / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014465:Ultrasonics / Q000009:adverse effects*
D010108:Oxyhemoglobins; D000254:Sodium-Potassium-Exchanging ATPase