Partial purification and cleavage specificity of a site-specific endonuclease, SciNI, isolated from Spiroplasma citri.
A site-specific endonuclease, SciNI, has been partially purified from the plant pathogen Spiroplasma citri. The enzyme recognizes the sequence 5'-G-C-G-C-3' and cleaves between the first G and C. 3'-C-G-C-G-5' SciNI is an isoschizomer of HhaI, but generates DNA fragments with 5' rather than 3' single-stranded protrusions.
D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D013149:Spiroplasma / Q000201:enzymology*; D013379:Substrate Specificity
D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases