[Cytological and cytochemical studies of cell cultures infected with the avirulent viral mutant MK 35 of the pseudorabies virus].
Cytological and cytochemical studies were carried out of cell cultures of chick embryo fibroblasts infected with the vaccinal mutant strain MK 35 (4.8 X 10(7) PFU) of the Pseudorabies virus. It was found that the first cytologic changes in the infected cultures presented definite focal character. In the first hours of infection several rounded cells were included only, while later on an increasing number of cells were involved, and the foci grew in size. The cytoplasma of the infected cells contained inclusions which were small, spherical, basophilic, and Foelgen positive; six hours later the cytoplasmatic inclusions became larger, oval, acidophilic, surrounded by a brighter area, and were Fölgen-negative. Parallel to the cytologic changes in the infected cell cultures there set in metabolic disturbances and changes in the enzymatic activity. The infected cells showed enhanced lactate dehydrogenase, diphosphopyridin-nucleotide diaphorase, thiamine pyrophosphatase, and alkaline phosphatase activity and suppressed succinate dehydrogenase and acid phosphatase activity. These studies were said to reveal new biologic properties of the vaccinal mutant virus.
D000818:Animals; D002642:Chick Embryo; D011558:Herpesvirus 1, Suid / Q000472:pathogenicity*; D006651:Histocytochemistry; D009154:Mutation; D011557:Pseudorabies / Q000201:enzymology / Q000473:pathology*; D013997:Time Factors; D014774:Virulence; D014776:Virus Cultivation
Tsitologichni i tsitokhimichni prouchvaniia na kletŭchni kulturi, zarazeni s avirulentniia virusen mutant MK 35 na Pseudorabies virus.