Biochemical, biological, and immunological properties of chemically deglycosylated human choriogonadotropin.
A chemical method of deglycosylation of human choriogonadotropin (hCG) was used to assess the role of carbohydrate moiety in the maintenance of quaternary structure and functional parameters such as receptor binding, immunological activity, and in vitro biological response. Treatment of purified hCG with anhydrous HF at 0 degrees C for 60 min was effective in removing more than 75% of the carbohydrate moiety. This extent of deglycosylation altered its chromatographic characteristics as revealed by retarded behavior on Sephadex G-100 and failure to be retained on concanavalin A-Sepharose. The electrophoretic heterogeneity present in native hCG was markedly reduced by deglycosylation. The deglycosylated hCG was stable in the lyophilized form and retained its quaternary structure as revealed by the fluorescence probe 8-anilino 1-naphthalene sulfonic acid, receptor binding, and immunological activities. Unlike receptor binding and immunological activities, which were fully retained, the ability of the hormone to stimulate cyclic AMP accumulation in vitro in rat interstitial cells was completely abolished.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002241:Carbohydrates / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D006801:Humans; D006858:Hydrofluoric Acid; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D010053:Ovary / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011974:Receptors, LH; D013053:Spectrophotometry; D013737:Testis / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D006063:Chorionic Gonadotropin; C034923:HCG, deglycosylated; D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D006858:Hydrofluoric Acid