Effect of ceftriaxone on Pseudomonas aeruginosa and Staphylococcus aureus in broth, serum, and in combination with human polymorphonuclear leukocytes.
We investigated the antibacterial activity of ceftriaxone at concentrations of 1/4 X minimum inhibition concentration (MIC), 1 X MIC and 4 X MIC against a serum-resistant Pseudomonas aeruginosa and a serum-resistant STaphylococcus aureus strain in broth, serum, and in combination with leukocytes. Killing effect of ceftriaxone in broth was significantly better than in serum; ceftriaxone improved leukocyte bactericidal activity without serum on P. aeruginosa, but not on S. aureus. The antibacterial activity of ceftriaxone was most effective in combination with leukocytes and serum, achieving a marked bactericidal effect already at subinhibitory ceftriaxone concentrations.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000494:pharmacology; D002443:Ceftriaxone; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D002443:Ceftriaxone; D002439:Cefotaxime