An inhibitor of (Na+, K+)-ATPase produced by Streptomyces pseudovenezuelae MF722-02; purification and properties.
A culture product of Streptomyces pseudovenezuelae MF722-02, with a molecular formula of C29H32N2O7, was isolated as yellow needles from culture broths and mycelia of the organism by means of a series of solvent extraction, column chromatography and crystallization. The antibiotic is active against some Gram-positive bacteria, inhibits growth in vitro of cells of mouse leukemia L-1210, prolongs the life span of mice inoculated with the leukemia cells, enhances deoxycholate-induced hemolysis in vitro and inhibits (Na+, K+)-ATPase in vitro.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D003840:Deoxycholic Acid; D006461:Hemolysis / Q000187:drug effects; D007939:Leukemia L1210 / Q000188:drug therapy / Q000648:ultrastructure; D051379:Mice; D008837:Micrococcus / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013302:Streptomyces / Q000032:analysis*
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003840:Deoxycholic Acid; D000254:Sodium-Potassium-Exchanging ATPase