title: Independence of H-2 and viral antigens on the cell surface and absence of H-2 antigens on murine leukemia virus and mouse mammary tumor virus particles.
abstract: By indirect immunoelectron microscopy we tested for the presence of H-2 antigens on murine mammary tumor virus (MMTV) and murine leukemia virus (MuLV) particles. The association of H-2 antigens and viral antigens on the virus-infected cell surface was investigated with antibody-induced redistribution. Mammary tumor cells and leukemia cell lines with different H-2 genotypes and carrying different MuMTV or MuLV were used. No H-2 antigens could be demonstrated on the envelope of MMTV and MuLV particles, even after the permeabilization of their envelopes with saponin. On the surface of virus-infected cells antibody-induced patching or capping of the viral antigens did not result in copatching or cocapping of the H-2 antigens. In the reciprocal tests no co-redistribution of viral antigens with H-2 antigens was seen. Our experiments failed to show any physical association between H-2 antigens and MMTV or MuLV antigens on the cell surface.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D006183:H-2 Antigens / Q000276:immunology*; D007158:Immunologic Techniques; D008325:Mammary Neoplasms, Experimental / Q000648:ultrastructure; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice; D008854:Microscopy, Electron; D012190:Retroviridae / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D006183:H-2 Antigens