Changes in the protein composition of Epstein-Barr Virus-negative lymphoma cells after EBV-conversion.
Two-dimensional protein separation, combining high resolution isoelectric focussing with SDS gel electrophoresis, was applied to analyse Epstein-Barr Virus (EBV)-induced changes in the protein composition of lymphoma cells. Total lysates of the EBV-negative Burkitt lymphoma lines Ramos and BJAB were compared with their permanently converted EBV-genome positive sublines, after 35S-methionine labelling. Five out of seven EBV-infected lines showed a 68k protein, which was absent from both EBV-free parental lines, and was identified as a glycoprotein (gp 68) by metabolic sugar labelling. We conclude that presence of this protein is a direct or indirect consequence of the presence of the EBV genome.
D002051:Burkitt Lymphoma / Q000378:metabolism* / Q000382:microbiology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004586:Electrophoresis; D006023:Glycoproteins / Q000378:metabolism*; D004854:Herpesvirus 4, Human; D006801:Humans
D006023:Glycoproteins; C037501:glycoprotein GP 68