Inhibition of rabbit tissue kininase by anti-(endo-oligopeptidase A) antibodies.
Highly purified rabbit brain endo-oligopeptidase A injected into goats produced, after 60 days of immunization, antisera that specifically inhibit purified rabbit brain endo-oligopeptidase A. An immunoreactive kininase having the same specificity as rabbit brain endo-oligopeptidase A for bradykinin was detected in several rabbit tissues. The highest amount of this immunoreactive kininase was found in the 25000 g supernatant fraction (S fraction) of heart, liver, skeletal muscle, ovary, brain and testis homogenates, corresponding to 89, 86, 78, 59, 56 and 53% respectively of the whole kininase activity found in the S fraction.
D000818:Animals; D001920:Bradykinin / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007703:Peptidyl-Dipeptidase A / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D014018:Tissue Distribution
D007106:Immune Sera; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin