Recombination between two TnA transposon sequences oriented as inverse repeats is found less frequently than between direct repeats.
Inverse repeats of the transposon Tn2660 in either a ColE1 or an R6K replicon, with or without inversions of the parental DNA sequences between the repeats, show no detectable (less than 2%) evidence of recombination between the repeats after 60 generations of growth in either recA or RecA+ hosts. In contrast, attempts made to construct plasmids which carry two direct repeats by in vitro cleavage and ligation in the recA host were unsuccessful, although homologous plasmids with inverse repeats could be constructed, and other plasmids were found consistent with products of recombination between the direct repeats of the transient intermediate structure. It is concluded that in recA or recA+ hosts recombination between direct repeats of a transposon is frequent, whereas recombination between inverse repeats of a homologous structure has not been observed. A model to explain this difference depends upon a mechanisms that produces a nick in only one of the pair of strands at the internal resolution site (IRS) sequence of the transposon.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics*; D008954:Models, Biological; D010957:Plasmids; D011995:Recombination, Genetic; D012093:Replicon; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI