[Isolation, purification and properties of restriction endonuclease EcoRII].
The restriction endonuclease Eco RII was isolated and purified to homogeneity. The isolation procedure involved the use of the E. coli strain B834/pSK323, containing the recombinant plasmide pSK323 which provides for the oversynthesis of Eco RII enzymes. Data from gel filtration and Na-DS electrophoresis suggest that the restriction endonuclease Eco RII is a protein made up of two subunits, each with molecular weight of 44 000.
D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010957:Plasmids
D004274:DNA, Recombinant; D046911:Macromolecular Substances; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Vydelenie, ochistka i kharakteristika restriktsionnoiéndonukleazy EcoRII.