Comparative mitogenic effects of herpes simplex virus and mycoplasma on murine lymphocytes.
Previous work indicated that herpes simplex virus type 1 (HSV-1) is a mitogen for mouse spleen cultures, as monitored by uptake of 3H-thymidine. We observed variable responses of mouse spleen cultures to different viral preparations. The variable responses, which did not follow normal dose-response relationships, were not due to HSV-1 strain differences, altered response kinetics or the presence or absence of defective viral particles, but to mycoplasma contamination of viral stocks. Mycoplasma-free (MF) HSV-1 stocks were prepared by transfection of MF NHF cells with HSV-1 DNA. MF HSV-1 infection of spleen cultures resulted in a five- to sixfold stimulation of DNA synthesis and stimulation of a polyclonal antibody response. Heat treatment (56 degrees C for 1 h) and antibiotics were used to distinguish mycoplasma and HSV-1-induced spleen culture mitogenic responses. The mycoplasma-induced mitogenic activity was found to be heat labile and sensitive to gentamicin and chloramphenicol. In contrast, the HSV-1 induced response was not affected by gentamicin or chloramphenicol and heat treatment resulted in only a 50% loss of activity.
D000818:Animals; D000917:Antibody Formation; D004247:DNA / Q000096:biosynthesis; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D008938:Mitosis; D009174:Mycoplasma / Q000502:physiology*; D018139:Simplexvirus / Q000502:physiology*; D013154:Spleen
D004247:DNA