DNA sequence of the gene coding for Escherichia coli ribonuclease H.
The gene for Escherichia coli ribonuclease H has been studied by use of a plasmid which contains a segment of the E. coli chromosome. The genomic DNA was subcloned from pLC28-22 to pBR322 by use of various restriction enzymes. Such subcloning limited the RNase H gene to a piece of DNA no longer than 760 base pairs. Cells bearing plasmids containing the RNase H gene produce as much as 10-15 times the normal amount of RNase H without any drastic effect on maintenance of the plasmid or cell growth. DNA sequence analysis has permitted the prediction of a protein whose molecular weight is 17,559 (155 amino acid residues). The predicted sequence was confirmed by amino acid analysis, NH2-terminal amino acid sequence, and size determination of highly purified RNase H.
D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D004722:Endoribonucleases / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D010957:Plasmids; D016914:Ribonuclease H
D000596:Amino Acids; D004269:DNA, Bacterial; D004722:Endoribonucleases; D016914:Ribonuclease H