Evidence for increased release of prostaglandins of E-type in response to orthodromic stimulation in the guinea-pig superior cervical ganglion.
Prostaglandins of the E type (PGEs) stimulate cyclic adenosine 3',5'-monophosphate (cAMP) biosynthesis both in isolated preparations of rat, guinea-pig and rabbit superior cervical ganglia (SCG) and in calf SCG slices. Electrical stimulation of preganglionic nerve fibers of the guinea-pig SCG remarkably increased PGE release and cAMP biosynthesis. These effects were blocked by reducing the Ca2+ to Mg2+ ratio in the incubation medium. Atropine (1 microM) and phentolamine (10 microM) inhibited PGE biosynthesis and significantly reduced cAMP levels.
D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000032:analysis*; D004558:Electric Stimulation; D005728:Ganglia, Sympathetic / Q000032:analysis / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D011458:Prostaglandins E / Q000378:metabolism* / Q000502:physiology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D009435:Synaptic Transmission
D011458:Prostaglandins E; D000242:Cyclic AMP