Localization of human placental opiate binding sites on the syncytial brush border membrane.
Opiate binding sites were measured in different placental membrane fractions which were characterized by marker enzyme analysis and electron microscopic examination. The distribution pattern of opiate binding sites in the different fractions closely parallels that of placental alkaline phosphatase. These results clearly show that opiate binding sites are mainly located on the syncytial brush border membrane. The opiate binding sites found on microvillus membrane fraction have the same pharmacological characteristics as the Kappa opiate binding site previously characterized on placental crude membrane fraction.
D000469:Alkaline Phosphatase / Q000378:metabolism; D001192:Arylsulfatases / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005048:Etorphine / Q000378:metabolism; D005260:Female; D006801:Humans; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011957:Receptors, Opioid / Q000032:analysis*; D017473:Receptors, Opioid, kappa; D014327:Trophoblasts / Q000378:metabolism*
D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa; D005048:Etorphine; D008058:Dihydrolipoamide Dehydrogenase; D000469:Alkaline Phosphatase; D001192:Arylsulfatases