A simple method for shortening a plasmid genome using a system of plasmid cointegration mediated by a Tn3 mutant.
D001483:Base Sequence; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014176:Protein Biosynthesis; D013045:Species Specificity
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes