Characterization of Alu family repetitive sequences which flank human beta-type globin genes.
Heteroduplex mapping has determined the size, location, and orientation of three Alu family sequences from the human beta-type globin gene cluster. Two of these sequences have the same orientation. One (231 bp long) is 2 kb to the 5' side of the B gamma-globin gene and the other (222 bp) is l kb 5' to the pseudo-beta-l-globin gene. The third (300 bp), 3-4 kb 3' to the pseudo-beta-l-globin gene, has the opposite orientation. Their orientations relative to five previously characterized Alu sequences from this cluster have been established. One of these, 2.5 kb 5' to the epsilon-globin gene, was shown by Southern blot hybridization to be similar but not identical to other family members, whereas the region separating it from a neighbouring inverted repeat is not widely distributed in the human genome.
D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005914:Globins / Q000235:genetics*; D006801:Humans; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D009692:Nucleic Acid Heteroduplexes; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes