title: [Human immune interferon: production and action].
abstract: The most marked production of immune interferon by human peripheral blood leukocytes and splenocytes stimulated with phytohemagglutinin (PHA) and staphylococcal enterotoxin A (SEA) was shown to be achieved when lymphoid cells are propagated under conditions of constant sparing mixing on roller apparatus at a temperature of 37 degrees +/- 0.5 degrees C. The resulting interferon was sensitive to low pH, thermolabile, inactivated by treatment with trypsin, and not neutralised by antisera to human alpha- and beta-interferons. The antiviral properties with regard to vesicular stomatitis and Semliki Forest viruses were practically similar in PHA- and SEA-induced interferon and human alpha- and beta-interferons. The capacity to inhibit colony formation by HeLa cells was 30 times higher in gamma-interferon than the antiproliferative activity of alpha- and beta-interferons.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004768:Enterotoxins / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects / Q000382:microbiology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012672:Semliki forest virus; D013154:Spleen / Q000187:drug effects / Q000382:microbiology; D013211:Staphylococcus aureus; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus
chemical_list: D004768:Enterotoxins; D010835:Phytohemagglutinins; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma
vernacular_title: Chelovecheskiĭ immunnyĭ interferon: produktsiia i deĭstvie.