Conformity between heat-labile toxin genes from human and porcine enterotoxigenic Escherichia coli.
The genes encoding the heat-labile toxin of Escherichia coli were isolated by recombinant DNA methods from enterotoxigenic E. coli recovered from a human and a piglet with diarrhea. With restriction endonucleases, a fine-structure map was made for the toxin genes. Both genes were found to be highly homologous within the toxin-coding DNA, but the surrounding DNA sequences were found to be quite divergent. Analysis of in vitro-derived mutants demonstrated that the cistrons for the toxin proteins were located at the same sites on each restriction enzyme map.
D000818:Animals; D001427:Bacterial Toxins; D004262:DNA Restriction Enzymes; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006801:Humans; D009154:Mutation; D013045:Species Specificity; D013552:Swine / Q000382:microbiology
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli; D004262:DNA Restriction Enzymes