Effect of different collagenases on the isolation of viable hepatocytes from rat liver.
The isolation of viable hepatocytes from rat liver was found to depend on the source of collagenase (EC 3.4.24.3) more than any other single factor examined. Collagenase purified from Achromobacter iophagus/Bacillus polymyxa (collagenase/dispase) gave reproducibly high viability without the use of complex perfusion protocols.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D010450:Endopeptidases; D008099:Liver / Q000166:cytology*; D003012:Microbial Collagenase / Q000145:classification; D051381:Rats; D011919:Rats, Inbred Strains
D010450:Endopeptidases; D003012:Microbial Collagenase; C023139:dispase