Measurement of the metabolic burst in human neutrophils: a comparison between cytochrome c and NBT reduction.
Stimulation of human neutrophils with phorbol myristate acetate results in a metabolic burst, which can be measured as an enhanced cytochrome c reduction or NBT reduction. There is more NBT reduction than cytochrome c reduction. When cytochrome c and NBT are simultaneously present the reduction of each is about the same as when either cytochrome c or NBT is present. Whereas cytochrome c reduction is completely annihilated by externally added superoxide dismutase, NBT reduction is diminished to a lesser extent under the same conditions. It is concluded that cytochrome c reduction only measures extracellularly released superoxide, whereas NBT may be reduced by extracellular superoxide or other molecules as well; thus NBT measures another aspect of the metabolic burst.
D003574:Cytochrome c Group / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism*
D003574:Cytochrome c Group; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate