A monoclonal antibody with specificity for leukemic cells transformed by defective avian leukemia viruses.
Mouse anti-chicken monoclonal antibodies were raised against an avian myeloblastosis virus (AMV)-transformed myeloblastic leukemic cell line. One monoclonal antibody, S1-37 (IgG2a), reacted with producer and nonproducer myeloblastic leukemia cell lines transformed by AMV and by E-26 virus, but it did not react with chicken fibroblasts infected with RAV-2, MAV-2, MAV-1, or RAV-7. S1-37 also did not react with normal chicken hemopoietic cells, except for yolk sack macrophages and a small population of embryonal and adult bone marrow cells that morphologically resembled macrophages. Cytotoxicity studies of GM-CFU, the normal stem cell population of the granulocytic macrophage lineage, indicated that these cells lack the surface antigen recognized by S1-37. Immunoprecipitation studies of 125I surface-labeled myeloblastic leukemic cells indicated that S1-37 binds a 42,000 Mr polypeptide. The possible role of this polypeptide in the process of transformation and differentiation of chicken myeloid cells is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001354:Avian Leukosis Virus; D009189:Avian Myeloblastosis Virus; D001854:Bone Marrow Cells; D002460:Cell Line; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D006825:Hybridomas; D007942:Leukemia, Experimental / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D015017:Yolk Sac / Q000166:cytology
D000911:Antibodies, Monoclonal