[Purification of lipase of microbial origin by silochrome chromatography].
The possibility of purifying lipase from Geotrichum asteroides by chromatography with a modified silochrome used as sorbent has been explored. The paper presents a scheme of adsorption and subsequent desorption of lipase from octylsilochrome which requires that ethylene glycol, Na-deoxycholate and Triton X-100 be used as eluents. Triton, the last in the sequence, eluates the major portion of the enzyme. This eluent can be removed from the lipase solution by means of ultrafiltration through the Ripore-4 membrane in the presence of glycerol.
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D005782:Gels; D005848:Geotrichum / Q000201:enzymology*; D008049:Lipase / Q000302:isolation & purification*; D003904:Mitosporic Fungi / Q000201:enzymology*; D058428:Silica Gel; D012822:Silicon Dioxide; D014461:Ultracentrifugation / Q000379:methods
D005782:Gels; D058428:Silica Gel; D012822:Silicon Dioxide; D008049:Lipase
Ochistka lipazy mikrobnogo proiskhozhdeniia khromatografieĭ na silokhrome.