Expression in Streptomyces ambofaciens of an Escherichia coli K-12 gene which confers resistance to hygromycin B.
We have constructed two plasmid vectors (pKC293 and pKC305) that can replicate in Escherichia coli K-12 and Streptomyces ambofaciens. These shuttle vectors were used to demonstrate the expression of two E. coli genes, hygromycin B (Hm) resistance and Tn5 neomycin (Nm) resistance, in S. ambofaciens.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006921:Hygromycin B / Q000494:pharmacology*; D019868:Kanamycin Kinase; D009355:Neomycin / Q000494:pharmacology; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*
D000900:Anti-Bacterial Agents; D006921:Hygromycin B; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D009355:Neomycin