Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.
We have prepared heteroduplexes between two plasmids that carry, in the same orientation, two H-2 cDNA inserts, 1.15 and 1.0 kilobase long, respectively. Their sequences encode two distinct class I transplantation antigens of the mouse and differ by 8% of their nucleotides. Molecules with a rearranged array of restriction sites were found after transformation and cloning in an Escherichia coli recA- host. Nucleotide sequences showed that the rearranged molecules derived their nucleotides from the two parental strands. Thus, correction of these complex heteroduplexes takes place in E. coli and probably involves repair mechanisms. It provides the basis for a mutational process in which several nucleotides (amino acids) can be altered in a single event. It also offers a practical means of making genetic variants. Several other implications are discussed.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic
D004274:DNA, Recombinant; D006183:H-2 Antigens; D004247:DNA; D004262:DNA Restriction Enzymes