title: Estradiol-17 beta and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one production by isolated ovarian follicles of amago salmon (Oncorhynchus rhodurus) in response to mammalian pituitary and placental hormones and salmon gonadotropin.
abstract: The effects of mammalian pituitary and placental hormones and dibutyryl cyclic AMP (cAMP) on the production of estradiol-17 beta and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (17 alpha, 20 beta-diOHprog) by amago salmon (Oncorhynchus rhodurus) ovarian follicles in vitro were compared with partially purified chum salmon (Oncorhynchus keta) gonadotropin (SGA). Estradiol-17 beta production was examined using midvitellogenic ovarian follicles; SGA was the most effective hormone tested; at a dose of 1 microgram/ml it stimulated the production of approximately 4 ng/ml estradiol-17 beta. Ovine LH (o-LH) was about 0.5% as effective as SGA. Taking into account the relative purity of the preparations used, ovine FSH (o-FSH) was almost as effective as o-LH. Both HCG and bovine TSH (b-TSH) stimulated estradiol-17 beta production at high doses. Porcine ACTH (p-ACTH), ovine GH (o-GH), and ovine PRL (o-PRL) induced very modest increases in estradiol-17 beta; cAMP was highly effective. 17 alpha, 20 beta-DiOHprog production was examined using full-grown immature oocytes which were capable of maturing in vitro in response to 1 microgram/ml SGA. o-LH was about 0.5% as effective as SGA. o-FSH, b-TSH, and HCG appeared to be less effective in stimulating 17 alpha, 20 beta-diOHprog production, compared to estradiol-17 beta. o-GH and o-PRL induced marginal increases in production and p-ACTH had a very modest inverse dose-response effect.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006908:Hydroxyprogesterones / Q000096:biosynthesis*; D007986:Luteinizing Hormone / Q000494:pharmacology; D010053:Ovary / Q000378:metabolism*; D010908:Pituitary Hormones, Anterior / Q000494:pharmacology*; D012474:Salmon / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D006908:Hydroxyprogesterones; D010908:Pituitary Hormones, Anterior; C021012:17 alpha,20 beta-dihydroxypregn-4-en-3-one; D004958:Estradiol; D003994:Bucladesine; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin