Asymmetric transcription of mouse mammary tumor virus genes in vivo and in vitro.
Mouse mammary tumor virus (MMTV) DNA fragments were cloned into M13 bacteriophage, and the single-stranded recombinant phage DNAs were used as strand-specific nucleic acid hybridization probes to measure synthesis of plus (genomic) and minus strands of MMTV RNA in cultured cell lines and in cell-free preparations of nuclei. Pulse-labeling studies showed that synthesis of MMTV RNA in three different cell lines was highly asymmetric. In nuclear preparations from a cloned line of MMTV-infected rat hepatoma cells, elongation of nascent MMTV RNA chains and initiation of new MMTV RNA chains with nucleoside (beta-S)triphosphates were also highly asymmetric.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005814:Genes, Viral; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012367:RNA, Viral