Oxygen-derived radicals from Trypanosoma cruzi-stimulated human neutrophils.
This study provides biochemical and electron spin resonance spectroscopic evidence that contract of human polymorphonuclear leukocytes with antibody-coated Trypanosoma cruzi triggers the respiratory burst. Oxygen consumption, superoxide anion and hydrogen peroxide release were stimulated under conditions of polymorphonuclear leukocyte-mediated killing. This stimulation did not occur under non-killing conditions when antibody was omitted. A common mechanism of cytotoxicity of human polymorphonuclear leukocytes against different T. cruzi forms is suggested by the triggering of the respiratory burst by antibody-coated epimastigotes and trypomastigotes.
D003602:Cytotoxicity, Immunologic; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D010101:Oxygen Consumption; D014349:Trypanosoma cruzi / Q000378:metabolism*
D005609:Free Radicals; D006861:Hydrogen Peroxide