Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
Sodium channels and receptors to serotonin and kainate were 'transplanted' from human brain into frog oocytes, by isolating messenger RNA from a fetal brain, and injecting it into Xenopus laevis oocytes. The mRNA was translated by the oocyte and induced the appearance of functional receptors and channels in its membrane. This approach renders drug- and voltage-operated channels of the human brain more amenable to detailed study.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004558:Electric Stimulation; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D007608:Kainic Acid / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D018092:Kainic Acid Receptors; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D014981:Xenopus
D007473:Ion Channels; D007608:Kainic Acid; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018092:Kainic Acid Receptors; D011985:Receptors, Serotonin; D012701:Serotonin