Positive-selection cloning vehicle useful for overproduction of hybrid proteins.
Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed for regulated overproduction of hybrid proteins.
D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis
D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI