Effect of interaction of heavy metals on (Na+ -K+) ATPase and the uptake of 3H-DA and 3H-NA in rat brain synaptosomes.
The effect of interaction of Mn2+, Pb2+ and Cd2+ on (Na+ -K+) ATPase and uptake of labelled dopamine (3H-DA) and labelled noradrenaline (3H-NA) were studied in vitro in rat brain synaptosomes. The inhibition of (Na+ -K+) ATPase by Pb2+ and Cd2+ alone was concentration dependent, however, Mn2+ had almost no effect on the activity of this enzyme. Interaction of Cd2+ with either Pb2+ or Mn2+ was most powerful in inhibiting the activity of synaptosomal transport ATPase. Lower concentrations of Pb2+ increased while higher concentrations inhibited synaptosomal uptake of 3H-DA and 3H-NA. Lower concentrations of Cd2+ increased the uptake of 3H-DA while at concentrations of 100 microM, the uptake was inhibited, this metal had strong inhibitory effect on the uptake of 3H-NA. Mn2+ had inhibited the uptake of labelled amines. Interaction of Mn2+ with Pb2+ or Cd2+ produced inhibition on the uptake of 3H-DA and 3H-NA. The results of the uptake of biogenic amines in the presence of metal ions apparently had no correlation with the activity of (Na+ -K+) ATPase which is involved in the active transport of cations across cell membranes.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002104:Cadmium / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D007854:Lead / Q000494:pharmacology; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013574:Synaptosomes / Q000378:metabolism*; D014316:Tritium
D008670:Metals; D002104:Cadmium; D014316:Tritium; D007854:Lead; D008345:Manganese; D000254:Sodium-Potassium-Exchanging ATPase; D004298:Dopamine; D009638:Norepinephrine