AKR thymic lymphomas involving mink cell focus-inducing murine leukemia viruses have a common region of provirus integration.
Newly acquired proviruses related to a mink cell focus-inducing murine leukemia virus were detected in low copy number in restriction endonuclease-digested DNAs from thymic lymphomas of AKR/J mice. These extra proviruses were not present in DNAs of either normal thymus or leukemic brain tissues. Extra tumor-specific DNA fragments generated by restriction endonucleases either were identical in size or fell into similar size classes, suggesting a common site(s) of provirus integration. Characterization of extra EcoRI DNA fragments for mink cell focus-inducing viral sequences revealed that all of them contained large terminal repeat sequences and that a significant number represented proviruses with deletions.
D000406:AKR murine leukemia virus / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000235:genetics*; D007942:Leukemia, Experimental / Q000382:microbiology; D008223:Lymphoma / Q000382:microbiology*; D051379:Mice; D008806:Mice, Inbred AKR / Q000382:microbiology*; D013953:Thymus Neoplasms / Q000382:microbiology*
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004262:DNA Restriction Enzymes