[Maintenance of 2S retinol receptor in the retina of rats in injuries induced by visible light].
Male Wistar rats (140-150 g) were subjected to constant fluorescent light exposure (400 ft-cd) of 24 hrs-21 day; contents of rhodopsin, total soluble proteins, 2 S cytosolic receptor as well as the morphological (light microscopy) characteristics were studied in retina. After 24 hrs exposure content of rhodopsin was decreased by 82%, after 48 hrs--by 37% as compared with control level, and decreased down to zero value within the 20 day. The content of total cytosol proteins decreased more slowly and within 3 weeks constituted 68-72% as compared with the control level. There were progressive dystrophic changes of rods and cones within the third day, culminating in their destruction. 2 S receptor was determined by incubation of the cytosol with 2.0-6.0. 10(-8) M of 3H-retinol followed by sucrose gradient fractionation. During all the periods studied the 2 S receptor peak was observed. The data obtained suggest the maintenance of molecular mechanisms of vitamin A functioning in damaged retina.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D007700:Kinetics; D008027:Light / Q000009:adverse effects*; D008297:Male; D011832:Radiation Injuries / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D012164:Retinal Diseases / Q000378:metabolism* / Q000473:pathology; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism
D011956:Receptors, Cell Surface; D012177:Retinol-Binding Proteins; C027928:retinol binding protein receptor; D012243:Rhodopsin
Sokhranenie 2S-retseptora retinola v setchatke krys pri povrezhdaiushchem deĭstvii vidimogo sveta.