title: Effect of the DNA-binding domain of poly(ADP-ribose)synthetase on accurate transcription initiation in a HeLa cell lysate.
abstract: The effect of papain-digested fragments of poly(ADP-ribose)synthetase on accurate transcription initiation by RNA polymerase II was studied using a HeLa cell lysate. It was found that the DNA-binding domain of the enzyme, like the intact enzyme, preferentially suppressed random transcription initiation, whereas the automodification domain had no effect on transcription.
mesh_terms: D004247:DNA / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D009244:NAD+ Nucleosidase / Q000502:physiology*; D010206:Papain; D010446:Peptide Fragments / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology*; D012319:RNA Polymerase II / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D010446:Peptide Fragments; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D012319:RNA Polymerase II; D009244:NAD+ Nucleosidase; D010206:Papain