[Combination of radioimmunological and immunoelectron microscopic methods for detecting hepatitis A virus].
The possibility of combined performance of radioimmunoassay (RIA) and immune electron microscopy (IEM) in one preparation using protein A of Staphylococcus aureus for hepatitis A virus (HAV) detection in fecal specimens from hepatitis A patients within a short time (40-50 min) has been demonstrated. In the examinations of one preparation by RIA and IEM, their sensitivity was found to be approximately similar. According to RIA, a high content of HAV antigen was observed in those preparations where in addition to typical particles there were structures resembling individual fragments of empty HAV particles coated with antibody.
D000956:Antigens, Viral / Q000032:analysis; D005243:Feces / Q000382:microbiology; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods; D013205:Staphylococcal Protein A; D013997:Time Factors; D014771:Virion / Q000302:isolation & purification
D000956:Antigens, Viral; D013205:Staphylococcal Protein A
Kombinatsiia radioimmunologicheskogo i immunoélektronno-mikroskopicheskogo metodov dlia obnaruzheniia virusa gepatita A.