The interactive effects of fluoride and N-formyl-L-methionyl-L-leucyl-L-phenylalanine on superoxide production and cAMP levels in human neutrophils.
The kinetics of superoxide (O2-) production and intracellular cAMP levels were monitored in human neutrophils incubated in vitro with sodium fluoride and the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP). F- activation of both the O2- -generating system, NAD(P)H oxidase, and adenylate cyclase was characterized by a prolonged lag period of 8 to 10 min at 37 degrees C. Adenylate cyclase agonists or cAMP analogues which inhibited FMLP-induced O2- bursts did not affect O2- production of F- -activated cells. Prior treatment of cells with F- suppressed the short rapid burst elicited by FMLP but not the binding of the tripeptide. FMLP reciprocally decreased the lag period of the F- -induced burst by 40 to 50% and, in the case of cells incubated at temperatures below 37 degrees C, increased the rate of O2- production. A similar potentiating effect of FMLP on F- -induced elevation of intracellular cAMP levels was observed.
D000262:Adenylyl Cyclases / Q000097:blood; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000097:blood*; D005459:Fluorides / Q000097:blood*; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000097:blood*; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D019255:NADPH Oxidases; D009504:Neutrophils / Q000378:metabolism*; D012969:Sodium Fluoride / Q000097:blood; D013481:Superoxides / Q000097:blood*; D013997:Time Factors
D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012969:Sodium Fluoride; D002772:Cholera Toxin; D000242:Cyclic AMP; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D000262:Adenylyl Cyclases; D005459:Fluorides