A polypeptide containing 55 amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins.
The receptor for polymerized human and chimpanzee albumins has been identified on a hepatitis B surface antigen polypeptide of approximately 31,000 daltons. The polypeptide, designated P31, is composed of the major polypeptide of hepatitis B surface antigen (P22) and an additional, as yet unidentified, amino acid sequence. We split P31 with cyanogen bromide and obtained a polypeptide of approximately 8000 daltons (P8) that contained carbohydrate. P8 could bind to polymerized human and chimpanzee albumins, but not to polymerized albumins from animals without susceptibility to hepatitis B virus. The amino acid composition of P8 closely resembled that of 55 amino-acid sequence coded by the pre-S region in the deoxyribonucleic acid of hepatitis B virus. Using monoclonal antibody against P8, a solid-phase sandwich radioimmunoassay was developed for the specific determination of hepatitis B surface antigen bearing the receptor for polymerized albumin in the serum of patients with hepatitis B virus infection.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003488:Cyanogen Bromide / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase / Q000276:immunology; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D002679:Pan troglodytes / Q000097:blood; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification*; D011108:Polymers; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D010455:Peptides; D011108:Polymers; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D004259:DNA-Directed DNA Polymerase; D003488:Cyanogen Bromide