Involvement of DNA polymerase alpha in host cell reactivation of UV-irradiated herpes simplex virus.
Aphidicolin is a potent inhibitor of both host cell DNA polymerase alpha and herpes simplex virus (HSV)-induced DNA polymerase but has no effect on DNA polymerases beta and gamma of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase alpha in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aphr was markedly inhibited by 1 microgram of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase alpha is involved in the repair of UV-irradiated HSV DNA.
D016590:Aphidicolin; D002472:Cell Transformation, Viral / Q000528:radiation effects*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004224:Diterpenes / Q000494:pharmacology*; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011247:Pregnancy; D018139:Simplexvirus / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay
D004224:Diterpenes; D016590:Aphidicolin; D004257:DNA Polymerase II