Ribosome changes induced by irradiation of isolated rat liver in vitro.
Changes produced in ribosome populations by gamma irradiation of isolated livers (rat) are reported. The main effect was the increase in free ribosomes due to the liberation of membrane bound ribosomes. The radiation apparently injures the binding-site of the large ribosomal subunit, detaching its structural proteins. The disassembled ribosomes are rapidly catabolized by cell RNase. The amount in total and free ribosomes of the livers as a function of the radiation dose was assessed. The relative insensitivity to radiation of the free ribosome pool is discussed in terms of its implications for repair and transformation of the cells.
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D008099:Liver / Q000528:radiation effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000528:radiation effects*