The effect of free and protected oils on the digestion of dietary carbohydrates between the mouth and duodenum of sheep.
Sheep fitted with rumen and re-entrant duodenal cannulas were given diets of approximately 200 g hay and 400 g concentrate mixture alone, or supplemented daily with 40 g linseed or coconut oils free or protected with formaldehyde-casein in a 5 x 5 Latin-square arrangement. Chromic oxide paper was given as a marker at feeding time and passage to the duodenum of neutral-detergent fibre (NDF) and different sugars were estimated from the values for constituent:marker at the duodenum. Contributions of microbial carbohydrates to these flows were estimated from amounts of RNA present. The carbohydrate composition of mixed rumen bacteria from sheep rumen digesta were similar regardless of diet. Of the sugars entering the duodenum all the rhamnose and ribose and 0.51, 0.24 and 0.35 of the mannose, galactose and starch-glucose respectively, were contributed by the microbes. Virtually all the arabinose, xylose and cellulose-glucose were contributed by the diet. For sheep receiving the basal ration, coefficients of digestibility between mouth and duodenum, corrected where necessary for microbial contribution, were 0.95, 0.66, 0.67, 0.62, 0.45 and 0.51 for starch-glucose, mannose, arabinose, galactose, xylose and cellulose-glucose respectively. Corresponding values when free-oil-supplemented diets were given were 0.95, 0.55, 0.38, 0.55, 0.01 and -0.02 respectively. Values for diets supplemented with linseed oil or coconut oil did not differ significantly. Addition of protected oils to the basal feed also resulted in depressed digestibilities of dietary structural sugars but to a far lesser extent than those observed with the free oils. Apparent digestibility of NDF was altered in the same direction as those of the main structural sugars, averaging 0.50, 0.17 and 0.29 in animals receiving the basal, free-oil-supplemented or protected-oil-supplemented diets respectively. The reasons for the difference between NDF and discrete carbohydrate analytical totals are discussed.
D000818:Animals; D001419:Bacteria / Q000378:metabolism; D003059:Cocos; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology*; D004043:Dietary Fiber / Q000378:metabolism*; D004063:Digestion / Q000187:drug effects*; D004386:Duodenum / Q000378:metabolism; D005260:Female; D008043:Linseed Oil / Q000494:pharmacology; D009821:Oils / Q000494:pharmacology*; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology; D012756:Sheep
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004043:Dietary Fiber; D009821:Oils; D008043:Linseed Oil