Cotransport of proline and Li+ in Escherichia coli.
Uptake of Li+ induced by the addition of proline to a cell suspension of Escherichia coli was detected using an Li+-selective electrode. This Li+ uptake was inhibited by L-azetidine 2-carboxylic acid, a competitive inhibitor of the proline transport system. Thus, direct evidence for Li+-proline cotransport via the proline transport system was obtained. Kinetic parameters of the Li+ uptake were determined.
D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008094:Lithium / Q000378:metabolism* / Q000494:pharmacology; D011392:Proline / Q000378:metabolism* / Q000494:pharmacology; D011522:Protons
D011522:Protons; D001383:Azetidinecarboxylic Acid; D011392:Proline; D008094:Lithium