Peptide E: opiate receptor binding profile in the rat brain membrane assay. Comparison with beta-endorphin.
Beta-endorphin (beta-EP) and peptide E were compared in respect to their binding potency in the rat brain membrane by radioreceptor binding assay using tritiated human beta-EP, [D-Ala2,D-Leu5]-enkephalin (DADLE), dihydromorphine (DHM) and ethylketocyclazocine (EKC) as primary ligands. When the potency of beta h-EP was chosen to be 100%, peptide E was equipotent with beta-EP in displacing DHM (95%) and EKC (103%) less potent for competing with beta h-EP (60%) and least active (7%) for displacing DADLE. It may be concluded that peptide E binds preferentially with the opiate mu and kappa receptors in the rat brain membrane.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*
D004723:Endorphins; D011957:Receptors, Opioid; C030962:adrenal opioid peptide E