[Fragmentation of the lactose-protease plasmid in lactose-negative derivatives of Streptococcus lactis and S. lactis ssp. diacetylactis].
DNA analysis showed the presence of plasmids in all 29 strains of mesophilic lactic streptococci. Their number ranged from 1 to 9 and their molecular weight extended from 1.8 to 45 megadaltons (Md). Comparison of the plasmid profiles from 9 strains of Streptococcus lactis, 3 strains of S. lactis ssp. diacetylactis and from their Lac-,Prt-variants was carried out. In most of the strains, a single plasmid of 27 to 45 Md carried both the lactose and protease (lac and prt) determinants. In Lac- variants, this composite plasmid may at times be lost; in most derivatives, this plasmid was fragmented into several smaller plasmids. Fragmentation resulted in the inactivation of lac genes and often of prt genes; sometimes prt genes remained intact in one of these new plasmids. Fragmentation of the plasmid may also take place without inactivation of these metabolic genes. Restriction analysis of the lactose-protease plasmid from 3 strains of S. lactis and from one Lac-variant confirmed the plasmid fragmentation phenomenon, and led to a proposal for physical mapping of the plasmid and preliminary localization of the lac-prt genes.
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000032:analysis / Q000235:genetics*; D007786:Lactose Factors; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D010447:Peptide Hydrolases
Fragmentation du plasmide lactose-protéase chez des dérivés lactose-négatifs de Streptococcus lactis et de S. lactis ssp. diacetylactis.