[Use of SDS and saponin detergents for the demonstration and purification of (Na+K+)ATPase in sheep kidney].
Incubation of membrane fragments from sheep kidney outer medulla in detergents such as SDS or saponin revealed increased specific (NA+) ATPase activity. Measurement of enzyme phosphorylation from [gamma 32P] ATP showed irreversible alteration of EP to (Na+) ATPase activity ratio when SDS is used as unmasking agent. These results were found with unmasked and purified enzyme.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D012503:Saponins; D012756:Sheep; D012967:Sodium Dodecyl Sulfate; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification* / Q000378:metabolism
D012503:Saponins; D012967:Sodium Dodecyl Sulfate; D010042:Ouabain; D000255:Adenosine Triphosphate; D010744:Phosphoric Monoester Hydrolases; D000254:Sodium-Potassium-Exchanging ATPase
A propos de l'utilisation des détergents SDS et saponine pour le démasquage et la purification de la (Na+K+)ATPase de rein de mouton.