Heterogeneity in the physicochemical properties of deoxycholate-solubilized benzodiazepine receptors from calf cerebral cortex.
The hydrodynamic behaviour of benzodiazepine receptors solubilized by deoxycholate from calf cerebral cortex reveals two molecular forms. The Stokes radii are 46.5 A and 67.2 A, and the sedimentation coefficients are 10.9 S and 14.6 S. The calculated apparent molecular weights and frictional ratios suggest either two nearly globular proteins of ca. 200K and 400K daltons each, or two ca. 300K daltons proteins which differ significantly in their degree of asymmetry. The benzodiazepine binding site is located on ca. 51K daltons component(s) in both forms.
D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000378:metabolism*; D003840:Deoxycholic Acid; D005445:Flunitrazepam / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D011963:Receptors, GABA-A; D012995:Solubility
D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D003840:Deoxycholic Acid; D001569:Benzodiazepines; D005445:Flunitrazepam