title: A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.
abstract: The cellular oncogene c-Ki-ras is amplified 30- to 60-fold in cells of the mouse adrenocortical tumour Y1. The amplified oncogene is located in double minute chromosomes and in a homogeneously staining chromosomal region, common karyotypical anomalies of tumour cells. The amounts of c-Ki-ras specific mRNA and of the protein (p21) encoded by the amplified gene are correspondingly elevated. Amplification and enhanced expression of cellular oncogenes may contribute to the genesis and/or maintenance of at least some naturally occurring tumours.
mesh_terms: D000306:Adrenal Cortex Neoplasms / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000502:physiology; D004262:DNA Restriction Enzymes; D005784:Gene Amplification; D007621:Karyotyping; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010957:Plasmids; D012334:RNA, Neoplasm / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012334:RNA, Neoplasm; D004262:DNA Restriction Enzymes