title: The bacteriophage lambda O and P protein initiators promote the replication of single-stranded DNA.
abstract: A soluble enzyme system that specifically initiates lambda dv plasmid DNA replication at a bacteriophage lambda replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and phi X174 to a duplex form. This chain initiation on single-stranded templates is novel in that it is absolutely dependent on the lambda O and P protein chromosomal initiators and on several Escherichia coli proteins that are known to function in the replication of the lambda chromosome in vivo, including the host dnaB, dnaG (primase), dnaJ and dnaK replication proteins. Strand initiation occurs at multiple sites following an O and P protein-dependent pre-priming step in which the DNA is converted into an activated nucleoprotein complex containing the bacterial dnaB protein. We propose a scheme for the initiation of DNA synthesis on single-stranded templates in this enzyme system that may be relevant to strand initiation events that occur during replication of phage lambda in vivo.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D003090:Coliphages / Q000235:genetics; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D010957:Plasmids; D013698:Templates, Genetic; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: C024013:DNA replication complex protein, Bacteriophage lambda; D004277:DNA, Single-Stranded; D014764:Viral Proteins; D004262:DNA Restriction Enzymes