Isolation and characterization of monopinocytotic vesicles containing polyomavirus from the cytoplasm of infected mouse kidney cells.
Monopinocytotic vesicles containing polyomavirus were isolated from the cytoplasm of mouse kidney cells infected with polyomavirus using sucrose density gradients. Nonenclosed, membrane-associated virions released by the action of neuraminidase separated from vesicle-enclosed virions in the sucrose gradient. Marker enzyme assays indicated the derivation of the vesicle membrane from the plasma membrane of the cell. The 125I-labeled virus enclosed in the vesicle sedimented more slowly in the gradient and was not observed unless infection and endocytosis had occurred. Detergent treatment of virion-containing vesicles caused the release of polyomavirus with sedimentation properties similar to those of purified polyoma virions. In addition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of virion proteins from vesicles containing virions demonstrated patterns of proteins similar to those of purified intact virions. Electron microscopy confirmed the presence of single intact virions inside vesicles. The study of these monopinocytotic virion-containing vesicles represents a further step in elucidating the early events of polyomavirus infection.
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D051379:Mice; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000494:pharmacology; D009940:Organoids / Q000382:microbiology* / Q000648:ultrastructure; D010873:Pinocytosis; D011120:Polyomavirus / Q000032:analysis / Q000302:isolation & purification* / Q000502:physiology; D014764:Viral Proteins / Q000032:analysis; D015678:Viral Structural Proteins
D003902:Detergents; D014764:Viral Proteins; D015678:Viral Structural Proteins; D009439:Neuraminidase