Gravimetric method for the determination of insoluble and soluble dietary fibres.
The present paper describes a simple method for the determination of the insoluble and soluble dietary fibres in food. In a first step, starch and proteins are removed by enzyme treatment. The insoluble dietary fibres are determined gravimetrically after centrifugation and washing of the residue with water, ethanol, acetone and diethyl ether. The water-soluble dietary fibres are recovered from the supernatant liquid of the centrifugation by dialysis, freeze-drying, and subsequent weighing. The method has been used for the determination of dietary fibres in by-products from the industry.
D004043:Dietary Fiber / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D012995:Solubility