Effects of forskolin and cholera toxin on cyclic AMP release in a neurotensin-secreting rat C-cell line.
The effects of forskolin and cholera toxin on the regulation of cAMP release were studied in a neurotensin-secreting rat C-cell line. The interaction of these agents with norepinephrine, a potent neurotensin secretagogue, was also investigated. Forskolin stimulated cAMP release 10(2)-10(3) fold while it increased neurotensin release 2-3 fold. Cholera toxin caused a 10(2)-10(3) fold increase in cAMP release and had no effect on neurotensin release. We conclude that the 44-2 C-cells provide a new model for studying the regulation of the concomitant (via forskolin) or independent (via cholera toxin) secretion of cyclic AMP and/or neurotensin.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology*; D005576:Colforsin; D000242:Cyclic AMP / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D009496:Neurotensin / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats
D004224:Diterpenes; D005576:Colforsin; D009496:Neurotensin; D002772:Cholera Toxin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D002118:Calcium; D009638:Norepinephrine