Effect of several nucleotides on the phosphorylating activities of the Rous-sarcoma-virus transforming protein pp60v-src and its cellular homologue, pp60c-src.
In the presence of ADP and GDP the tyrosine-phosphorylating activities of the viral as well as the cellular pp60src show a similar concentration-dependent inhibition in vitro. Addition of diadenosine 5',5"'-p1p4 tetraphosphate (Ap4A) to the kinase assay leads to an inhibition of the viral kinase activity, whereas the cellular kinase is not influenced.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000201:enzymology*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D015226:Dinucleoside Phosphates; D006153:Guanosine Diphosphate / Q000494:pharmacology; D051379:Mice; D009711:Nucleotides / Q000494:pharmacology*; D015688:Oncogene Protein pp60(v-src); D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D014764:Viral Proteins / Q000037:antagonists & inhibitors*
D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D009711:Nucleotides; D047428:Protein Kinase Inhibitors; D014764:Viral Proteins; D006153:Guanosine Diphosphate; C020733:diadenosine tetraphosphate; D000244:Adenosine Diphosphate; D015688:Oncogene Protein pp60(v-src)