Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2.
Evidence is presented showing that the 92,000-dalton glycoprotein (g92K) induced by herpes simplex virus (HSV) type 2 has properties distinct from those assigned to any other HSV glycoprotein. First, the carbohydrate composition and extent of sulfation differ from those of glycoproteins D and E. Second, two clonally unrelated monoclonal antibodies, AP1 and LP5, shown in this paper to specifically immunoprecipitate g92K, do not react with any of the known processed forms of glycoproteins B, C, D, and E. Third, by using HSV type 1/HSV type 2 intertypic recombinants and a simple radioimmunoassay, the target antigen of the two monoclonal antibodies was shown to map in the same region as g92K (0.846 to 0.924). Fourth, the intertypic recombinant R12-3 was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of infected cells to induce the HSV type 2 g92K and HSV type 1 gD and GE, whereas R12-1, which did not induce g92K, induced HSV-2 gE and an altered gD, providing genetic evidence that g92K is encoded, at least in part, by a different region of the genome from that encoding gD and gE.
D000818:Animals; D000936:Antigen-Antibody Complex; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D007106:Immune Sera; D007668:Kidney; D008970:Molecular Weight; D011863:Radioimmunoassay; D018139:Simplexvirus / Q000235:genetics*
D000936:Antigen-Antibody Complex; D006023:Glycoproteins; D007106:Immune Sera