Localization of provirus DNA of Rous sarcoma virus in the DNA of infected, transformed and tumour cells of chickens.
The integration of the RSV provirus genome into chicken cell DNA was studied by restriction analysis at various times after infection with RSV. One to two copies of provirus DNA per cell genome are integrated and integration occurs at different sites of DNA without apparent specificity, independently of the stage of infection and transformation of cells. The exogenous virus DNA is integrated into cell genome independently of the endogenous proviruses. The number of integrated proviruses does not correlate with the number of non-integrated proviruses in infected cells. No changes in structure and location of endogenous proviruses were found in spontaneous chicken tumours.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts; D001357:Sarcoma, Avian / Q000032:analysis*; D013997:Time Factors
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004262:DNA Restriction Enzymes