Topoisomerase I activities in L-1210 cell nuclei.
Topoisomerase was extracted from L-1210 nuclei by 0.35 M sodium chloride Extract fractionation by molecular weight revealed two activities. The activities were quantitatively determined using a technique based on the electrophoretic separation of 3H-labelled DNA product from substrate. This technique enables the determination of topoisomerase activity in extracts where nuclease activity is also present.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis; D009690:Nucleic Acid Conformation
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D004264:DNA Topoisomerases, Type I