Elemental sulfur: a novel inhibitor of adenylate kinase.
Elementary sulfur (S-8) is a novel, highly specific and potent inhibitor of adenylate kinase (AK). The inhibition of AK by S-8 is completely reversed by dithiothreitol and is accompanied by several fold increases in enzymatic activity. Sulfhydryl group interaction with S-8 does not appear to be the mechanism of AK inhibitor and the results of comparative inhibition studies with N-ethyl maleimide (NEM) indicates that NEM also does not interact with the sulfhydryl group of AK.
D000263:Adenylate Kinase / Q000037:antagonists & inhibitors*; D000818:Animals; D003402:Creatine Kinase / Q000037:antagonists & inhibitors; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D011817:Rabbits; D013455:Sulfur / Q000494:pharmacology*
D007527:Isoenzymes; D013455:Sulfur; D010770:Phosphotransferases; D003402:Creatine Kinase; D000263:Adenylate Kinase; D005033:Ethylmaleimide; D004229:Dithiothreitol