The localization of genes for HPRT, G6PD, and alpha-GAL onto the X-chromosome of domestic pig (Sus scrofa domesticus).
Pig--mouse somatic cell hybrids were obtained from fusion of HPRT--mouse cells (RAG) and pig lymphocytes. The pig-mouse hybrids examined apparently retained on the average only 9 to 15 pig chromosomes. Seven of the hybrid clones were karyotyped to determine the pig chromosome constitution, and the same hybrid clones were tested electrophoretically for the expression of pig hypoxanthine-guanine phosphoribosyltransferase (HPRT), glucose-6-phosphate dehydrogenase (G6PD), and alpha-galactosidase (alpha-GAL) phenotypes. All five of the hybrid clones which had retained the pig X-chromosome exhibited concordant expression of pig HPRT, G6PD, and alpha-GAL enzymes. These data indicate that the genes HPRT, G6PD, and alpha-GAL are located on the X-chromosome of the domestic pig.
D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006822:Hybrid Cells / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D051379:Mice; D012730:Sex Chromosomes; D013045:Species Specificity; D013552:Swine / Q000235:genetics*; D014960:X Chromosome; D000519:alpha-Galactosidase / Q000235:genetics*
D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D005696:Galactosidases; D000519:alpha-Galactosidase