title: Comparison of biological, biochemical, immunological, and immunochemical techniques for typing herpes simplex virus isolates.
abstract: In this study, 102 herpes simplex virus isolates were typed by cell culture selection (chicken embryo cells and guinea pig embryo cells [CE/GPE]), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) sensitivity, plaque reduction neutralization, and indirect immunofluorescence staining techniques. The percentages of agreement between the typing methods were as follows: BVDU sensitivity versus CE/GPE, 99% (99/102); CE/GPE and BVDU sensitivity versus neutralization, 32% (33/102); CE/GPE and BVDU sensitivity versus indirect immunofluorescence staining 17% (17/102). Results were easy to interpret when the CE/GPE and BVDU sensitivity systems were used. In contrast, when type-specific antisera prepared commercially were used, results were often obscure, even contradictory, because of antibody cross-reactions. Therefore, this study suggests that immunological and immunochemical methods that use presently available commercially prepared antisera cannot reliably differentiate herpes simplex virus type 1 from type 2.
mesh_terms: D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D006801:Humans; D009500:Neutralization Tests; D018139:Simplexvirus / Q000145:classification* / Q000187:drug effects; D010948:Viral Plaque Assay
chemical_list: C020235:brivudine; D001973:Bromodeoxyuridine