Targeted selection of recombinant clones through gene dosage effects.
The availability of multicopy plasmid vectors for the yeast Saccharomyces cerevisiae allows the selective amplification of individual segments of the genome. Increased dosage of particular genes results in overproduction of specific gene products and thereby confers resistance to certain metabolic inhibitors. Advantage was taken of this fact to isolate recombinant clones that increase the activities of the enzymes UDP-N-acetylglucosamine-1-P transferase and 3-hydroxy-3-methylglutaryl-CoA reductase.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005796:Genes; D005800:Genes, Fungal; D005822:Genetic Vectors; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017855:Transferases (Other Substituted Phosphate Groups)
D004274:DNA, Recombinant; D006903:Hydroxymethylglutaryl CoA Reductases; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C030012:UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase; D004262:DNA Restriction Enzymes