title: High-fat diets and fecal level of reductase and colon mucosal level of ornithine decarboxylase, beta-glucuronidase, 5'-nucleotidase, ATPase, and esterase in mice.
abstract: In one experiment Swiss mice were maintained on a 16 or 23% fat diet (laboratory chow with added fat, principally corn oil) or on laboratory chow alone (5.5% fat). In another experiment C57BL/1 mice were given a 23% fat diet (as above) or a low-fat diet (67% laboratory chow, 1.9% corn oil, and 31% starch; 5.5% fat). Colon mucosal samples were analyzed for several enzyme activities. In Swiss mice the analyses revealed the following: 1) Ouabain-insensitive ATPase was unaltered in male mice, but it rose significantly in females fed a high-fat diet (this effect was seen when a resuspended high-speed pellet was analyzed but not seen with the initial homogenate); 2) 5'-nucleotidase activity showed a significant stepwise increase with dietary fat; 3) nonspecific esterase activity tended to rise with a high-fat diet (not significant); 4) beta-glucuronidase levels were not altered by diet fat; and 5) ornithine decarboxylase levels were not altered by diet fat. In C57BL/1 mice analyses were done on ouabain-insensitive ATPase, 5'-nucleotidase, nonspecific esterase, and beta-glucuronidase, but no diet effects were seen. Fecal reductase activity was measured with the use of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride hydrate). A high-fat diet did not affect the activity in C57BL/1 mice, but it caused a significant rise in Swiss mice.
mesh_terms: D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003106:Colon / Q000201:enzymology*; D004041:Dietary Fats; D005243:Feces / Q000201:enzymology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012737:Sex Factors; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013045:Species Specificity
chemical_list: D004041:Dietary Fats; D009247:NADH, NADPH Oxidoreductases; C028796:iodophenyl-nitrophenyl-phenyltetrazolium reductase; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D005966:Glucuronidase; D000251:Adenosine Triphosphatases; D009955:Ornithine Decarboxylase; D000254:Sodium-Potassium-Exchanging ATPase