title: Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus.
abstract: Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are (formula: see text) with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages lambda and phi X174 and virus SV40.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D019605:Euryarchaeota / Q000201:enzymology*; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D004262:DNA Restriction Enzymes; C040837:endodeoxyribonuclease MaeII; C040838:endodeoxyribonuclease MaeIII; C040836:CTAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific