A serological and biochemical study of new field isolates of foot-and-mouth disease virus type A in Peru, 1975 to 1981.
Three foot-and-mouth disease virus type A isolates recovered from field outbreaks in the Department of San Martin, Peru, during the period 1975 to 1981 were compared with each other, and the South American vaccine strains A24 and A27, by complement fixation (CF), virus neutralization (VN) and polyacrylamide gel electrophoresis (PAGE). Complement fixation and VN tests gave comparable results distinguishing the field isolates from each other and from the vaccine strains. Analysis of the structural polypeptides by PAGE also showed clear differences between all the viruses examined. Samples from tissue culture passaged and mouse adapted strains of one of the field isolates gave identical patterns in PAGE, but differences were observed in the polypeptide pattern of the A24/BRA/55 strain and the Peru vaccine strain, which were serologically indistinguishable. Results illustrate a continued antigenic variation in an endemic area where vaccination has been used; however, asymmetric serological reactions between the A24 vaccine strain and the most recent field isolate indicated that a vaccine incorporating A24 should still give adequate protection.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D005537:Aphthovirus / Q000145:classification* / Q000276:immunology; D002417:Cattle; D003168:Complement Fixation Tests / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006168:Guinea Pigs; D051379:Mice; D009500:Neutralization Tests / Q000379:methods; D010568:Peru; D012703:Serotyping / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology
D000956:Antigens, Viral; D014765:Viral Vaccines