Enhanced recombination between lambda plac5 and mini-F-lac: the tra regulon is required for recombination enhancement.
F42lac recombination with lambda plac is normally 20-fold to 50-fold higher than recombination between lambda plac and a chromosomal lac gene. Transductional crosses with lambda plac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42lac.
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005814:Genes, Viral; D005838:Genotype; D007763:Lac Operon; D010957:Plasmids; D013045:Species Specificity
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes