[Characteristics of collagen preparations labelled in vitro by 14C-acetic anhydrides. Their use for the determination of collagenase activity and as radioactive markers].
A modified procedure in developed for acetylation by means of 14C-acetic anhydride of highly purified collagen I preparations. The acetylated collagen exhibited high specific radioactivity and was effectively hydrolyzed by bacterial collagenase. The 14C-acetylated collagen was used as a radioactive marker in electrophoretic analysis of labelled proteins.
D000085:Acetates; D000089:Acetic Anhydrides; D000107:Acetylation; D000818:Animals; D002250:Carbon Radioisotopes; D003013:Clostridium / Q000201:enzymology; D003094:Collagen / Q000302:isolation & purification / Q000378:metabolism*; D004586:Electrophoresis; D066298:In Vitro Techniques; D007553:Isotope Labeling; D003012:Microbial Collagenase / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D000085:Acetates; D000089:Acetic Anhydrides; D002250:Carbon Radioisotopes; D003094:Collagen; D003012:Microbial Collagenase
Kharakteristika preparatov kollagena, mechennogo in vitro 14C-uksusnym angidridom. Ikh ispol'zovanie dlia opredeleniia kollagenaznoĭ aktivnosti i v kachestve radioaktivnykh markerov.