Macrophage activation measured by changes in 5'-nucleotidase and alkaline phosphodiesterase I activities after infection of newborn and juvenile guinea pigs with mycobacterium microti.
Activation of alveolar, peritoneal, liver, and spleen macrophages was measured by decreased 5'-nucleotidase and elevated alkaline phosphodiesterase I activities in newborn and juvenile guinea pigs for up to 48 days after intracardiac or intranasal infection with Mycobacterium microti. Increased AP and reduced 5'-nucleotidase activity in cell lysates of macrophages appeared to be related to the carrier state during infection. Intranasal infection of 8-week-old guinea pigs with M. microti produced decreases of 5'-nucleotidase activity in alveolar macrophages during 1-8 weeks post infection and in macrophages from all four sources from 4-8 weeks.
D015720:5'-Nucleotidase; D000818:Animals; D000831:Animals, Newborn; D006168:Guinea Pigs; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology; D009164:Mycobacterium Infections / Q000201:enzymology / Q000276:immunology*; D009169:Mycobacterium tuberculosis; D009708:Nucleotidases / Q000378:metabolism*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology
D009708:Nucleotidases; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I