Inhibition by superoxide dismutase and catalase of the damage of isolated Leishmania mexicana amazonensis by phenazine methosulfate.
Phenazine methosulfate, a cationic electron carrier, inhibits the extracellular growth of promastigotes and the conversion of amastigotes into promastigote forms of Leishmania mexicana amazonensis. Growth inhibition and damage of extracellular parasites by PMS was counteracted by superoxide dismutase, a scavenger of the superoxide anion (O2-), and to a lesser extent, by catalase, a scavenger of hydrogen peroxide (H2O2). Inactivated dismutase and catalase were ineffective. Thus, damage of isolated L.m. amazonensis by phenazine methosulfate, involves the participation of O2- and H2O2. The role of the oxygen metabolites in the toxicity of phenazine methosulfate remains unknown. That O2- can damage the parasites is supported by the finding that superoxide dismutase also protected promastigotes from damage induced by oxygen intermediates generated by a xanthine-xanthine oxidase system. Killing of the parasites by crystal violet, a triphenylmethane, or basic blue 24, a phenothiazine, was not inhibited by superoxide dismutase.
D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D005840:Gentian Violet / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007891:Leishmania / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008751:Methylene Blue / Q000031:analogs & derivatives / Q000494:pharmacology; D008773:Methylphenazonium Methosulfate / Q000037:antagonists & inhibitors*; D010619:Phenazines / Q000037:antagonists & inhibitors*; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D019820:Xanthine; D014969:Xanthine Oxidase / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
D003574:Cytochrome c Group; D010619:Phenazines; D014970:Xanthines; D013481:Superoxides; C035184:new methylene blue; D019820:Xanthine; D008773:Methylphenazonium Methosulfate; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D005840:Gentian Violet; D008751:Methylene Blue