[Cloning of Bacillus subtilis 168 genes compensating for the defect in mutations for thymidine phosphorylase and uridine phosphorylase in Escherichia coli cells].
On the basis of Bacillus subtilis DNA and vectors pBR325, pBR322, a collection of hybrid plasmids restoring the wild type in the double Escherichia coli mutant for thymidine phosphorylase (deoA) and uridine phosphorylase (udp) has been obtained. In spite of the fact that in Bac. subtilis, in contrast to E. coli, phosphorolytic cleavage of thymidine and uridine is catabolized by one enzyme-pyrimidine nucleoside phosphorylase (pdp gene), some plasmids caused the appearance of the activity similar to thymidine phosphorylase (deoA type) in E. coli cells. Plasmids of the deoA type as well as many of plasmids causing the appearance in E. coli cells of the activity of the pdp type were not able to transform cells of the Bac. subtilis pdp mutant, strain PC315, to the wild type. Moreover, on the basis of PC315 DNA, hybrid plasmids have been obtained giving the activity of the pdp type. The suggestion of the possible presence in Bac. subtilis 168 genome of non-expressible cryptic genes of the deoA and pdp type is discussed.
D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005838:Genotype; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010430:Pentosyltransferases / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D013939:Thymidine Phosphorylase / Q000235:genetics*; D014169:Transformation, Bacterial; D014543:Uridine Phosphorylase / Q000235:genetics*
D004269:DNA, Bacterial; D010430:Pentosyltransferases; D014543:Uridine Phosphorylase; D013939:Thymidine Phosphorylase
Klonirovanie genov Bacillus subtilis 168, kompensiruiushchikh defekt mutatsiĭ po timidinfosforilaze i uridinfosforilaze v kletkakh Escherichia coli.