Structural study on the active site of the collagenase from Hypoderma lineatum.
The collagenase from the larvae Hypoderma lineatum is a serine proteinase sequentially related to the trypsin family. The tryptic peptide containing the serine residue of the active site, labelled with [3H] diisopropylfluorophosphate was isolated and determined to be Ser-Pro-Cys-Phe-Gly-Asp-Ser-Gly-Gly-Pro-(Phe-Ser)-Lys. It is highly conservative with respect to the corresponding peptide in other serine proteinases related to trypsin.
D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004175:Diptera / Q000201:enzymology*; D003012:Microbial Collagenase / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D003012:Microbial Collagenase