[Immunomorphological identification of myoepithelial cells in mixed tumors of the mammary gland in dogs].
Myoepithelial cells (MC) in mixed tumors of the mammary gland in dogs were identified with the Coons indirect method with the aid of monospecific antiserum to smooth muscle myosin. In 2 of 5 observations, anaplastic carcinoma and adenocarcinomas were detected in the presence of a mixed tumor. Immunochemical study of the myoepithelium demonstrated varying fluorescence intensity and ununiform pattern of MC distribution in tumor tissue of the mammary gland. MC were detected in lobular structures in the form of islet accumulations or diffuse vegetations from cells with a poorly fluorescent rim of the cytoplasm. In the ducts with papillary epimyoepithelial proliferations, MC occupied the peripheral position, showing bright fluorescence of the cytoplasm and hypertrophied processes. The fluorescence intensity decreased as MC displaced towards the stroma or lumen of the ducts. The changeability of specific staining of the myoepithelium may attest to different levels of cell differentiation. The presence of immature forms of MC in mixed tumors is likely to be due to the modulatory character of cell differentiation and is determined by the totality of factors that apparently play the triggerring part in derepression of the genes of specific synthesis of smooth muscle proteins in undifferentiated cells of the epithelium of the terminal ducts and alveoli.
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D002277:Carcinoma / Q000473:pathology; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005455:Fluorescent Antibody Technique; D008321:Mammary Glands, Animal / Q000473:pathology*; D009369:Neoplasms / Q000473:pathology / Q000662:veterinary*; D009373:Neoplasms, Germ Cell and Embryonal / Q000662:veterinary*; D009378:Neoplasms, Multiple Primary / Q000473:pathology; D003557:Phyllodes Tumor / Q000473:pathology*
Immunomorfologicheskaia identifikatsiia mioépitelial'nykh kletok v smeshannykh opukholiakh molochnoĭ zhelezy u sobak.