[Changes of the DNA synthesis and the amount of S-100 protein associated with the morphological differentiation of cultured glioma cells].
S-100 protein is a highly acidic protein unique to the nervous system and exists predominantly in the cytoplasm of glial cells in a water-soluble form. The exact biological function is still unknown in spite of its well-known biochemical properties. Some investigators have reported that the amount of S-100 protein in developing brains increased in proportion to the brain's development and differentiation. In order to clarify the relationship between S-100 protein and differentiation of glial cells the changes of DNA synthesis and the amount of the water-soluble S-100 protein were investigated on cultured rat glioma (C-6) cells in the course of the morphological differentiation induced by dibutyryl cyclic AMP (dbc-AMP). C-6 cells were cultured in Eagle's minimum essential medium supplemented with 10% fetal calf serum and incubated in a humidified atmosphere of 5% CO2-95% room air at 37 degrees C. Dbc-AMP was added to the media at the concentration of 1 mM, and the media were changed every 48 hrs. A flow cytometric analysis of DNA histogram patterns was performed to investigate the changes of DNA synthesis using a Cytofluorograf FC 4800 A-50 (Bio/Physics). The changes of the amount of S-100 protein were examined by micro-complement fixation assay as described by Levine using rabbit antisera against bovine S-100 protein. Dbc-AMP inhibited the growth of C-6 cells remarkably and induced morphological changes resembling normal astrocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002453:Cell Cycle; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000032:analysis*; D013997:Time Factors
D004273:DNA, Neoplasm; D009418:S100 Proteins; D003994:Bucladesine