title: Cloning and cleavage site mapping of DNA from bovine herpesvirus 1 (Cooper strain).
abstract: Sequences representative of most of the bovine herpesvirus 1 (Cooper strain) DNa were cloned in the plasmid vector pBR322 at the HindIII site. EcoRI, HpaI, and BamHI restriction endonuclease sites were mapped in each of the cloned fragments, and this information was used to construct a restriction endonuclease cleavage site map of the entire viral genome for the four enzymes.
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D005822:Genetic Vectors; D006564:Herpesviridae / Q000201:enzymology / Q000235:genetics*; D006566:Herpesviridae Infections / Q000662:veterinary; D010957:Plasmids
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes