Formation of phenolic and dihydrodiol metabolites of benzo(a)pyrene in freshly isolated human hair follicles occurs independently.
Freshly isolated hair follicles of 20 adult non-smoking volunteers were assayed for formation of phenolic and dihydrodiol metabolites of benzo(a)pyrene (BP). In each of a total of 14 experiments two volunteers were assayed simultaneously, and the ratios of both phenolic and dihydrodiol metabolites of BP between the two individuals were determined. It was obvious that the mean interindividual variation in formation of phenolic metabolites was greater than the variation in formation of dihydrodiol metabolites. No correlation existed between the amount of both types of metabolites formed. These observations indicate that for detection of differences in carcinogen metabolism to assess individual susceptibility to the carcinogenic action of polycyclic aromatic hydrocarbons (PAH), measurements of phenolic and dihydrodiol metabolites of BP are not interchangeable.
D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism*; D004101:Dihydroxydihydrobenzopyrenes; D005260:Female; D006197:Hair / Q000378:metabolism*; D006801:Humans; D008297:Male; D010636:Phenols / Q000302:isolation & purification; D013329:Structure-Activity Relationship
D001580:Benzopyrenes; D002273:Carcinogens; D004101:Dihydroxydihydrobenzopyrenes; D010636:Phenols; C017228:benzo(a)pyrene 7,8-dihydrodiol; C020738:9,10-dihydro-9,10-dihydroxybenzo(a)pyrene; D001564:Benzo(a)pyrene; D001189:Aryl Hydrocarbon Hydroxylases