Stress-induced changes in plasma, pituitary and hypothalamic immunoreactive beta-endorphin: effects of diurnal variation, adrenalectomy, corticosteroids, and opiate agonists and antagonists.
Immunoreactive beta-endorphin (ir-beta EP) levels were determined in plasma, anterior pituitary (AP), neuro-intermediate lobe (N-IL) and hypothalamus (HT) of intact rats and adrenalectomized rats subjected to swimming stress. In intact rats, plasma ir-beta EP was elevated fourfold, and ir-beta EP levels in AP and N-IL significantly reduced after 30 min swimming; in contrast, HT ir-beta EP in these animals was not affected. In adrenalectomized animals, similar but even more marked changes were found in plasma and tissue ir-beta EP following stress. The effect of diurnal variation, morphine and naloxone on stress-induced changes in tissue ir-beta EP was also examined; in addition, the influence of acute and chronic treatment of adrenalectomized animals with dexamethasone or deoxycorticosterone was explored. Plasma ir-beta EP of rats swum in the afternoon was significantly higher than of those swum in the morning; no differences were detected in AP, N-IL or HT ir-beta EP. Acute morphine administration (10 mg/kg) did not affect levels of ir-beta EP in any tissue, baseline or post-stress; however, naloxone (10 mg/kg) consistently and significantly prevented the reduction in N-IL ir-beta EP otherwise found following prolonged stress. Both acute and chronic dexamethasone (2 and 20 micrograms) attenuated the stress-induced changes found in AP and plasma ir-beta EP in a dose-related manner; equivalent effects, however, were not seen in deoxycorticosterone (20, 200 micrograms) treated animals. In contrast, the stress-induced reduction found in N-IL ir-beta EP was influenced neither by acute nor chronic treatment with dexamethasone or deoxycorticosterone. These results suggest that changes found in AP and plasma ir-beta EP after swimming stress are glucocorticoid-sensitive, but do not involve pathways including opiate receptors; in contrast, though baseline levels of N-IL ir-beta EP are mineralocorticoid-sensitive, the changes after swimming stress are steroid-independent and involve the action of endogenous opioids.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000315:Adrenalectomy; D000818:Animals; D002940:Circadian Rhythm; D003907:Dexamethasone / Q000494:pharmacology; D004723:Endorphins / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology*; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000378:metabolism*; D001615:beta-Endorphin
D000305:Adrenal Cortex Hormones; D004723:Endorphins; D009294:Narcotics; D009270:Naloxone; D001615:beta-Endorphin; D009020:Morphine; D003907:Dexamethasone