[Passive hemagglutination reaction in differentiating foot-and-mouth disease viruses].
Experiments were carried out with the use of the passive hamagglutination reaction in the differentiation of foot-and-mouth disease viruses. The investigations made use of purified sera and specific IgG antibodies obtained through column chromatography. Conjugates were prepared with the use of bis-diazotized benzidine and glutaraldehyde. In experiments with conjugates prepared with IgG and glutaraldehyde standard and reproducible results were obtained. The use of the passive hemagglutination test has led to the successful differentiation of F. M. D. viruses of various origins. Comparative investigations with the complements-fixation test and the passive hemagglutination test have shown that the latter is more readily applicable and rapid as well as more sensitive.
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D005537:Aphthovirus / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods*; D007074:Immunoglobulin G / Q000302:isolation & purification; D012756:Sheep / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G
Reaktsiia pasivna khemaglutinatsiia pri diferentsirane na shapnite virusi.