Superoxide-dependent formation of hydroxyl radical catalyzed by transferrin.
Hydroxyl radicals are generated in the hypoxanthine-xanthine oxidase system in the presence of iron-saturated transferrin isolated from human serum. This has been demonstrated by colorimetrically measuring the hydroxylation of salicylic acid and by EPR using the spin trap DMPO (5,5-dimethyl-1-pyrroline-N-oxide). A Fenton-type Harber-Weiss reaction catalyzed by transferrin is proposed.
D000818:Animals; D002384:Catalysis; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006801:Humans; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D006900:Hydroxylation; D007042:Hypoxanthines / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D005609:Free Radicals; D006878:Hydroxides; D007042:Hypoxanthines; D014168:Transferrin; D013481:Superoxides; D017665:Hydroxyl Radical; D014969:Xanthine Oxidase; D010100:Oxygen