title: Capped and conserved terminal structures in human rotavirus genome double-stranded RNA segments.
abstract: Both 3'- and 5'-terminal structures of human rotavirus genome double-stranded RNA segments were determined. RNAs were labeled at the 3'-termini with [32P]pCp by incubation with RNA ligase and at the 5'-termini with [32P]phosphate by polynucleotide kinase or, in the case of 5' caps, with 3H by chemical modification with [3H]NaBH4. Examination of radiolabeled termini released by digestion with several base-specific RNases revealed that rotavirus RNA segments are base paired end-to-end and contain the same terminal structures: (formula; see text)
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D012321:DNA-Directed RNA Polymerases; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D005814:Genes, Viral; D006801:Humans; D009841:Oligonucleotides / Q000096:biosynthesis / Q000302:isolation & purification; D012315:RNA Caps / Q000032:analysis; D012367:RNA, Viral / Q000302:isolation & purification*; D012260:Ribonucleases; D012401:Rotavirus / Q000235:genetics* / Q000648:ultrastructure; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D009841:Oligonucleotides; D012315:RNA Caps; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D004720:Endonucleases; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases