Pertussis toxin decreases opiate receptor binding and adenylate inhibition in a neuroblastoma x glioma hybrid cell line.
Neuroblastoma X glioma hybrid cells NG108-15 were treated with a toxin derived from Bordetella pertussis. As compared to control cells grown in the absence of toxin, the inhibitory effects of opioid agonists upon cAMP formation were dose-dependently impaired by a non-competitive mechanism. Radioligand binding studies revealed that opioid agonist binding was dramatically reduced in toxin-treated membranes when tested in the presence of Na+/Mg++/GMP-PNP. Further, the potencies of guanine nucleotides to decrease opioid agonist binding were differentially modulated. These studies may facilitate our understanding of the mechanisms responsible for acute and chronic opiate effects.
D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001886:Bordetella pertussis; D002999:Clone Cells; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D037342:Pertussis Toxin; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism*; D010566:Virulence Factors, Bordetella
D000067956:Adenylyl Cyclase Inhibitors; D001427:Bacterial Toxins; D011957:Receptors, Opioid; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin