Expression of human placental-type alkaline phosphatase in primary breast cancer.
A monoclonal antibody (H317) with specificity for the heat-stable placental-type alkaline phosphatase (PI-ALP) isoenzyme has been used to investigate the occurrence of PI-ALP in patients with primary breast carcinoma. All pre-operative plasma samples were negative for PI-ALP in a sensitive solid-phase enzyme immunoassay with a lower limit of detection of 0.1 U/l. In contrast, using a peroxidase-anti-peroxidase staining technique on fixed tissue sections, as well as enzyme immunoassay on fresh tissue extracts, PI-ALP could be demonstrated in the carcinomatous tissue of all seven patients investigated.
D000232:Adenofibroma / Q000201:enzymology* / Q000276:immunology; D000469:Alkaline Phosphatase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000201:enzymology* / Q000276:immunology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000201:enzymology* / Q000276:immunology; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D006965:Hyperplasia / Q000201:enzymology / Q000276:immunology; D007114:Immunization; D051379:Mice; D010920:Placenta / Q000201:enzymology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase