Rapid three-step purification procedure for isolation of the C1q component of human complement and its use in a solid-phase C1q binding assay.
A new procedure for the isolation of the C1q subcomponent of complement from human sera has been devised. The 3-step protocol employs DEAE Sephadex A-50, hydroxyapatite and Sephacryl S-200 chromatographies and can be performed within 9 h. It yields immunoglobulin-free homogeneous C1q protein with about 80% recovery. The isolated C1q protein is biologically active and may be used for the detection of circulating immune complexes in sera by the solid-phase C1q binding assay.
D000936:Antigen-Antibody Complex / Q000032:analysis; D001666:Binding Sites, Antibody; D002850:Chromatography, Gel / Q000379:methods*; D003166:Complement Activating Enzymes / Q000276:immunology / Q000302:isolation & purification*; D015922:Complement C1q; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites; D007105:Immune Complex Diseases / Q000175:diagnosis / Q000276:immunology; D007124:Immunoenzyme Techniques
D000936:Antigen-Antibody Complex; D006882:Hydroxyapatites; D015922:Complement C1q; D017886:Durapatite; D003166:Complement Activating Enzymes