Isolated interferon alpha-receptor complexes stabilized in vitro.
We describe the extraction and stabilization in vitro of discrete complexes of interferon and cellular receptor proteins. A homogeneous complex of Mr 230 000 was extracted at the time of peak receptor binding (30 min). Complex formation was specific for human interferon. At later times a second complex could also be extracted suggesting transfer of interferon to a second site.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002850:Chromatography, Gel; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D007700:Kinetics; D008214:Lymphocytes / Q000032:analysis / Q000378:metabolism; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D017471:Receptors, Interferon
D007370:Interferon Type I; D011956:Receptors, Cell Surface; D017471:Receptors, Interferon