A cleavable cross-linking reaction for protein carboxyl groups.
L-(+)-tartaric acid dihydrazide was coupled to the purple membrane from Halobacterium halobium with a water soluble carbodiimide. Gel electrophoresis analysis of the product revealed the formation of bacteriorhodopsin dimers, trimers and higher polymers. Most of the cross-links were removed by treatment with papain, demonstrating involvement of the carboxyl-rich carboxyl-terminal region of bacteriorhodopsin in the reaction. The cross-links were cleavable by periodate oxidation.
D001436:Bacteriorhodopsins; D002338:Carotenoids; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D010084:Oxidation-Reduction; D010206:Papain; D010504:Periodic Acid; D013644:Tartrates
D003432:Cross-Linking Reagents; D013644:Tartrates; D010504:Periodic Acid; D002338:Carotenoids; D001436:Bacteriorhodopsins; C039034:tartaric acid dihydrazide; C009288:metaperiodate; D010206:Papain