Promoter-probe plasmid for Bacillus subtilis.
We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in both E. coli and B. subtilis.
D001412:Bacillus subtilis / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D009876:Operon; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D001616:beta-Galactosidase