The nuclear 3,5,3'-triiodothyronine receptor in human leukaemic cell lines.
The 3,5,3'-triiodothyronine (T3) receptor has been studied in a series of continuously growing human leukaemic cell lines. High concentrations of receptor were found in the erythroblastoid cell line K-562. T3 was bound to the nuclei of these cells with an association constant of 3.4 X 10(9) M-1, and capacity 104 fmol/100 micrograms DNA, or 8700 molecules/nucleus. This capacity is comparable to that of rat liver or growth hormone producing cells (GH cells) in culture, and suggests that the K-562 cell line could be a useful model for the study of T3 action on erythroid differentiation.
D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000209:etiology; D004920:Erythropoiesis; D006801:Humans; D007938:Leukemia / Q000209:etiology / Q000378:metabolism*; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones / Q000502:physiology
D011956:Receptors, Cell Surface; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones