Sequence and structure of a methionine transfer RNA from mosquito mitochondria.
We have sequenced a methionine tRNA from mosquito mitochondria, and examined its structure using nucleases S1 and T1 under non-denaturing conditions. The sequence is highly homologous to a putative initiator methionine tRNA gene from Drosophila mitochondria. Its anticodon stem contains a run of three G-C base pairs that is characteristic of conventional initiator tRNAs; however, nuclease S1 analysis suggested an anticodon loop configuration characteristic of conventional elongator tRNAs. We propose that this tRNA can assume both initiator and elongator roles.
D000330:Aedes / Q000235:genetics*; D000818:Animals; D000926:Anticodon; D001483:Base Sequence; D002478:Cells, Cultured; D004720:Endonucleases; D008928:Mitochondria / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics* / Q000302:isolation & purification; D015719:Single-Strand Specific DNA and RNA Endonucleases
D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; C026464:tRNA(m)(Met), methionine-; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases