title: A vector that uses phage signals for efficient synthesis of proteins in Escherichia coli.
abstract: I have developed a plasmid vector pCQV2 for high-level expression of proteins in E. coli. The plasmid contains a very strong promoter and translation start point from bacteriophage lambda, and a restriction endonuclease site in which a gene may be placed under control of these initiation signals. The plasmid also contains the gene for a temperature-sensitive repressor of the lambda promoter, allowing 100-fold induction of protein expression. The vector pCQV2 has been used to synthesize SV40 small t antigen at the level of 5-10% of total E. coli protein, representing an order-of-magnitude improvement over previous methods.
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D000957:Antigens, Viral, Tumor; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D005814:Genes, Viral; D005822:Genetic Vectors; D009876:Operon; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D001616:beta-Galactosidase