Cloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto rho 11 temperate bacteriophage.
A HindIII fragment harboring the intact spo0C gene of Bacillus subtilis was cloned with rho 11 temperate bacteriophage as a vector. Transformation experiments with the DNA from rho 11 dspo0C+ specialized transducing phage showed that the spo0C gene resides on a 5.3-megadalton fragment generated by HindIII digestion. The 5.3-megadalton fragment also contains the intact spo0A gene, but not spoIIIA, spoIIIB, or spoIVB.
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D005798:Genes, Bacterial; D013171:Spores, Bacterial / Q000502:physiology; D014161:Transduction, Genetic
D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII