title: Skeletal muscle differentiation in Wilms' tumor. Antibody identification and explant culture.
abstract: We studied a case of Wilms' tumor with focal areas of well-differentiated skeletal muscle by immunoperoxidase staining using anti-skeletal muscle myosin and by tissue culture explant of the tumor. Strong immunostaining occurred in areas of well-differentiated muscle but also occurred in focal areas where the cells were undifferentiated. The primary explant of this tumor was attempted using standard serum-containing culture media and a serum-free hormonally defined culture medium. While serum-containing culture media gave rise to a fibroblastlike cell population, the serum-free hormonally defined medium produced cells of myoid origin. Ultrastructurally, all stages of early myogenesis from myoblasts to myotubules were observed in this primary explant grown in serum-free medium.
mesh_terms: D000906:Antibodies / Q000032:analysis; D002454:Cell Differentiation; D046508:Culture Techniques; D006801:Humans; D007223:Infant; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*; D009218:Myosins / Q000276:immunology; D009396:Wilms Tumor / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*
chemical_list: D000906:Antibodies; D009218:Myosins