Effects of repeated treatment of hamster ear epidermis and cheek-pouch mucosa with 12-0-tetradecanoylphorbol-13-acetate.
The effects of repeated stimulation of hamster oral mucosa and epidermis with 12-0-tetradecanoylphorbol-13-acetate (TPA), were examined using in vitro assays of glycolysis and amino acid incorporation. The response of control and hyperplastic tissues to epinephrine and cAMP, and their rates of incorporation of histidine and leucine, were also examined. Repeated treatment with TPA in the concentration range of 1 X 10(-8) to 1 X 10(-5) M resulted in increased rates of glycolysis, amino acid incorporation and mitosis in both tissues. During the induction of hyperplasia, a loss of the normal epithelial response to epinephrine was observed at Day 8, suggesting re-establishment of mechanisms controlling homeostasis. Application of TPA resulted in a reduction in the amount of histidine incorporated relative to leucine.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D009061:Mouth Mucosa / Q000187:drug effects* / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D000596:Amino Acids; D010704:Phorbols; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate; D004837:Epinephrine