Effect of oxygen on the induction of tryptophan hydroxylase by adrenergic agents in organ cultures of rat pineal glands.
Rat pineal glands, cultured under 95% air and 5% CO2, lost 50% of their tryptophan hydroxylase activity within 5 h. This loss was accelerated by the addition of cycloheximide or puromycin to the medium. Activity was, however, largely maintained in 95% O2 and 5% CO2. Under these conditions, L-noradrenaline (100 microM), L-isoproterenol (10 microM), and dibutyryl cyclic AMP (1 mM) induced enzyme activity. They failed to do so when 95% air and 5% CO2 was used. Noradrenaline induced serotonin N-acetyltransferase activity with either atmosphere.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010100:Oxygen / Q000494:pharmacology*; D010870:Pineal Gland / Q000187:drug effects / Q000201:enzymology*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014365:Tryptophan Hydroxylase / Q000096:biosynthesis*
D011691:Puromycin; D003994:Bucladesine; D003513:Cycloheximide; D014365:Tryptophan Hydroxylase; D007545:Isoproterenol; D010100:Oxygen; D009638:Norepinephrine