Amino acid sequence studies on sheep liver fructose-bisphosphatase. II. The complete sequence.
The cyanogen bromide fragments of S-carboxymethylated fructose-bisphosphatase were purified. The amino acid sequences of the small fragments were determined by the dansyl-Edman method. The large fragments were subjected to proteolytic digestion to give smaller peptides more amenable for purification and sequencing by similar methods. Enzyme digests of the S-carboxymethylated enzyme gave overlap peptides containing the methionine residues. In conjunction with the amino acid sequence of the 60-residue N-terminal fragment previously determined on the S-peptide released by limited proteolysis with subtilisin the complete sequence of 336 residues was deduced. The sequence has been compared with the 335 residue sequence of pig kidney fructose-bisphosphatase and some areas of sequence for rabbit liver enzyme. The strong homology previously noted for the S-peptide sequence is maintained for the complete enzyme with only 34 changes in 336 residues when comparing the pig and sheep enzymes.
D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide; D006597:Fructose-Bisphosphatase / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D010455:Peptides / Q000032:analysis; D012756:Sheep
D010455:Peptides; D006597:Fructose-Bisphosphatase; D002268:Carboxypeptidases; D003488:Cyanogen Bromide