Cellular RNA is not degraded in interferon-treated HeLa cells after poliovirus infection.
A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or minus interferon treatment, followed by translation in a reticulocyte lysate indicates that cellular mRNAs remain active. These results suggest that gross degradation of cellular RNA does not occur in interferon-treated poliovirus-infected HeLa cells and that a non-specific nuclease induced by 2'-5' A is not responsible for the inhibition of protein synthesis observed.
D002472:Cell Transformation, Viral; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification
D007370:Interferon Type I; D012333:RNA, Messenger; D012334:RNA, Neoplasm