Effect of the serine-borate complex on the relative ability of leukotriene C4, D4 and E4 to inhibit lung and brain [3H] leukotriene D4 and [3H] leukotriene C4 binding: demonstration of the agonists' potency order for leukotriene D4 and leukotriene C4 receptors.
To define the potency order of the leukotrienes for inhibition of [3H] leukotriene D4 and [3H] leukotriene C4 binding, we investigated leukotriene C4, D4 and E4 competition with and without the serine-borate complex in guinea pig lung and brain homogenates. Without it, the rank order of their potency for inhibition of lung [3H] leukotriene D4 or [3H] leukotriene C4 binding was leukotriene C4 = leukotriene D4 greater than leukotriene E4. Presence of the complex altered the potency order for both competition studies: for the [3H] leukotriene D4 competition it was leukotriene D4 greater than leukotriene E4 = leukotriene C4 and for the [3H] leukotriene C4 competition it was leukotriene C4 much greater than leukotriene D4 greater than or equal to leukotriene E4.
D000818:Animals; D001667:Binding, Competitive; D001881:Borates / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D007700:Kinetics; D017999:Leukotriene E4; D008168:Lung / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018077:Receptors, Leukotriene; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D012694:Serine / Q000494:pharmacology*; D014316:Tritium
D001881:Borates; D011956:Receptors, Cell Surface; D018077:Receptors, Leukotriene; D013189:SRS-A; C040900:serine-borate complex; D014316:Tritium; D012694:Serine; D017999:Leukotriene E4