Nucleotide sequence of a reiterated rat DNA fragment.
A reiterated component of rat DNA was isolated by restriction with HindIII endonuclease and polyacrylamide gel electrophoresis. Sequence analysis revealed that the fragment was 179 nucleotides long. Unlike the known 370N reiterated rat DNA fragment which contained a high m5C content (2.7 mole%), this repetitive element contained a rather low m5C content (0.5 mole%). The present rat repetitive sequence appeared to be of alpha-type as shown by its significant homologies with alpha DNA sequences of African green monkey and human. The possibility of sequence heterogeneity is discussed.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D000882:Haplorhini; D006801:Humans; D008099:Liver / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004247:DNA; D004262:DNA Restriction Enzymes