Localization with the electron microscope of p27 Rous sarcoma virus internal protein in chicken embryo fibroblasts. A time study at different stages after infection.
Protein 76 (Pr76), the precursor of the so-called 'gag' protein of avian leukosarcoma viruses, has been localized in the light and electron microscope by using the immunoperoxidase technique in Rous sarcoma virus-infected chick embryo fibroblasts. The kinetics of the progression of the protein through the cell was studied at different times after infection. The protein was first found (after 8 h) on ribosomes located near the nucleus and then proceeded rapidly to the periphery of the cell. After 5-6 days, intracytoplasmic staining faded away and only the ribosomes close to the plasma membrane were stained. A possible association of a protein with the cytoskeleton is suggested.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts; D015683:Gene Products, gag; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism*
D015683:Gene Products, gag; D011498:Protein Precursors; D014764:Viral Proteins