Macrophage characteristics of a cell line isolated in vitro from a Moloney lymphoma.
A new cell line, LM-A, established in vitro from a murine Moloney lymphoma, is described. The line shows the cytologic, adherence, and phagocytic properties of normal macrophages. It forms specific rosettes with antibody-coated erythrocytes. The cells mediate antibody-dependent cellular cytotoxicity as assayed by release of radioactivity from 51Cr-labeled erythrocytes. The cytolytic reaction proved to be functionally distinct from the phagocytic process as demonstrated by enhanced cytolysis in the presence of iodo-acetamide, an inhibitor of phagocytosis. The line has Moloney murine leukemia virus (MLV)-related antigens as detected by membrane immunofluorescence. The LM-A line is useful for the study of macrophage functions and also provides an interesting tool for investigating the antigenic and immunogenic properties induced by MLV in a cell as relevant as the macrophage in the immune response.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D007942:Leukemia, Experimental / Q000276:immunology / Q000473:pathology; D008223:Lymphoma / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus; D010587:Phagocytosis; D012397:Rosette Formation; D009053:Sarcoma Viruses, Murine