The localization of 2':3'-cyclic nucleotide 3'-phosphodiesterase in bovine cerebrum by immunofluorescence.
The distribution of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in fixed-tissue sections and Percoll-isolated cells (oligodendrocytes) of bovine cerebrum was studied by immunofluorescent techniques. Fluorescence in tissue sections exposed to CNP antisera was restricted to oligodendrocyte-like cells. Also, Percoll-isolated cells (oligodendrocytes) were positively labeled with CNP antisera. This study has shown that CNP may be used as an antigenic marker for oligodendrocytes.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D002417:Cattle; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases