Comparison of sea urchin and human mtDNA: evolutionary rearrangement.
Clones of full-length mtDNA have been isolated from a Strongylocentrotus franciscanus recombinant DNA library by screening a cDNA clone of cytochrome oxidase subunit 1 mRNA. Restriction fragment cross-hybridization analysis shows the following difference in gene arrangement between sea urchin and human mtDNA. The 16S rRNA and cytochrome oxidase subunit 1 genes are directly adjacent in sea urchin mtDNA. These two genes are separated in human and other mammalian mtDNAs by the region containing unidentified reading frames 1 and 2. In spite of the difference in gene order, gene polarity appears to have been conserved. We conclude that the difference in gene order reflects a rearrangement that took place in the sea urchin lineage since sea urchins and mammals last shared a common ancestor.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D004274:DNA, Recombinant / Q000032:analysis*; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012617:Sea Urchins / Q000235:genetics*; D013045:Species Specificity; D013094:Spermatozoa / Q000032:analysis; D013737:Testis / Q000032:analysis
D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes