Solubilization of adrenal angiotensin II receptors with a zwitterionic detergent.
Active rat adrenal angiotensin II receptors have been solubilized with a zwitterionic detergent, 3 [(3-cholamidopropyl)-dimethylammonio] -1-propane sulfonate. The solubilized receptors retain a high affinity for angiotensin II (Kd = 1.9 nM) similar to the value observed in adrenal membrane particles (Kd = 1.2 nM). In addition, the binding-inhibition potency of several angiotensin II peptides is maintained unaltered, indicating a fully preserved specificity of the solubilized receptors.
D000311:Adrenal Glands / Q000032:analysis*; D000804:Angiotensin II / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002793:Cholic Acids; D003902:Detergents; D066298:In Vitro Techniques; D051381:Rats; D011945:Receptors, Angiotensin / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D012995:Solubility; D013997:Time Factors; D014021:Tissue Preservation
D002793:Cholic Acids; D003902:Detergents; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D000804:Angiotensin II; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate