Another potential artifact in the study of nucleosome phasing by chromatin digestion with micrococcal nuclease.
We show that, contrary to expectations, restriction enzyme cleavage of chicken erythrocyte nucleosome core particle DNA generates a series of distinct subnucleosome fragments. These fragments do not result from bulk nucleosome phasing in vivo, but arise from micrococcal nuclease cleavages internal to the core particle, at roughly 10-base pair intervals and at AT-rich sequences. Those 145-base pair DNA fragments remaining intact are a biased population in which the guanine content can fluctuate by as much as 10%, with a 10-base pair period. We suggest that these same considerations, when applied to a unique DNA sequence, are the true explanation for several previous claims for nucleosome phasing.
D000225:Adenine / Q000378:metabolism; D000818:Animals; D001482:Base Composition; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004912:Erythrocytes; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009707:Nucleosomes / Q000502:physiology*; D013941:Thymine / Q000378:metabolism
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D004262:DNA Restriction Enzymes; D008836:Micrococcal Nuclease; D000225:Adenine; D013941:Thymine