Blocked 5'-termini in the fragments of chromosomal DNA produced in cells exposed to the antitumor drug 4'-[(9-acridinyl)-amino]methanesulphon-m-anisidide (mAMSA).
Comparison of the sensitivity of DNA isolated from untreated and mAMSA-treated PY815 mouse mastocytoma cells to hydrolysis by E.coli 3'-exonuclease III and phage lambda or phage T7 5'-exonucleases show that the fragments of chromosomal DNA produced by mAMSA treatment have free 3'-OH termini and blocked 5'-termini.
D000609:Aminoacridines / Q000494:pharmacology*; D000677:Amsacrine; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D005090:Exodeoxyribonucleases / Q000378:metabolism; D006868:Hydrolysis; D012515:Mast-Cell Sarcoma / Q000235:genetics; D051379:Mice; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013604:T-Phages / Q000201:enzymology
D000609:Aminoacridines; D000970:Antineoplastic Agents; D000677:Amsacrine; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I