Host range mutants of human rhinovirus in which nonstructural proteins are altered.
Human rhinovirus type 2 did not replicate in nonpermissive mouse cells; the restriction was not in adsorption but in the early events of virus replication. Mutants which had been adapted to grow in mouse cells had the following characteristics: (i) no change in the structural protein, (ii) a larger nonstructural protein and its precursor protein, and (iii) an altered viral RNA synthesis. The altered nonstructural proteins correlated with a change in host range of the virus and may be involved in viral RNA synthesis.
D000818:Animals; D002213:Capsid; D005814:Genes, Viral; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D007739:L Cells; D051379:Mice; D008970:Molecular Weight; D009154:Mutation; D011498:Protein Precursors; D012367:RNA, Viral / Q000096:biosynthesis; D012229:Rhinovirus / Q000254:growth & development*; D014764:Viral Proteins / Q000096:biosynthesis / Q000502:physiology*
D011498:Protein Precursors; D012367:RNA, Viral; D014764:Viral Proteins