title: Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells.
abstract: HTLV was isolated in the human diploid fibroblast strain IMR90 directly from the leukemic cells of the Japanese ATL-patients. The infection was checked by the presence of virus-specific p24 and proviral DNA. The isolation was successful in three of four patients. The patient whose virus isolation was unsuccessful contained only a defective viral genome in his leukemic cells. The infected cells produced polykaryons when co-cultured with catS + L-cells. Human leukemia HL60 cells formed clusters adherent to the infected IMR90 cells but not to uninfected IMR90 cells. The former phenomenon was blocked by the ATLL patients' serum.
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D017977:Deltaretrovirus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D007564:Japan; D007938:Leukemia / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011233:Precipitin Tests; D012192:Retroviridae Infections / Q000382:microbiology* / Q000473:pathology; D014764:Viral Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins