A role for glutathione disulfide as a scavenger of oxygen radicals produced by 2,3-dichloro-1,4-naphthoquinone.
The addition of 2,3-dichloro-1,4-naphthoquinone (CNQ) to isolated mitochondria supplemented with GSSG resulted in a respiratory burst with the production of O2- and H2O2, and a decrease in the level of measurable disulfide. Superoxide generated by the xanthine oxidase system or by CNQ-treated mitochondria caused the reduction of GSSG to GSH. Both disulfide reductions were partially sensitive to exogeneous SOD. GSSG was also shown to interfere with the epinephrine - adrenochrome superoxide assay system. The findings reported herein support the conclusion that GSSG is capable of scavenging O2- and has the potential to scavenge other free radicals by a similar mechanism.
D000818:Animals; D002417:Cattle; D004837:Epinephrine / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D019803:Glutathione Disulfide; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism; D009285:Naphthoquinones / Q000378:metabolism*; D051381:Rats; D013481:Superoxides / Q000378:metabolism*
D009285:Naphthoquinones; D013481:Superoxides; C007332:dichlone; D005978:Glutathione; D019803:Glutathione Disulfide; D004837:Epinephrine