SV40-induced transformation and T-antigen production is enhanced in normal and repair-deficient human fibroblasts after pretreatment of cells with UV light.
Human fibroblasts irradiated with UV light were infected with simian virus 40 and tested either for transformation or T-antigen production. At UV doses that allowed approximately 5-10% of the irradiated cells to survive, the number of surviving transformed colonies increased. This result was confirmed by testing for T-antigen 96 h post infection by means of indirect immunofluorescence. Since these results were obtained for a normal cell line as well as for two UV excision repair-deficient ones (XP groups A and D), it was concluded that excision repair functions cannot play a decisive role in the events leading to increased transformation and T-antigen production. It is proposed that the relative increase of transformation and T-antigen production is the expression of host functions which are induced by DNA damage threatening cell survival.
D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002472:Cell Transformation, Viral / Q000528:radiation effects*; D004260:DNA Repair; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D013539:Simian virus 40 / Q000276:immunology; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000378:metabolism
D000957:Antigens, Viral, Tumor