title: Kinetics of the TEA and 4-AP sensitive K+ current in the slowly adapting lobster stretch receptor neurone.
abstract: The kinetics of the TEA and 4-AP sensitive K+ current (IK) in the slowly adapting lobster stretch receptor neurone were investigated in sub- and near-threshold voltage regions using electrophysiological and pharmacological techniques. In dynamic conditions IK was found to display both fast and slow reactions. These were attributed to a Hodgkin-Huxley type of K activation, and a slow type of K inactivation, respectively. The slow K inactivation could be shown to be unrelated to K+ flux dependent changes in intra- and pericellular K+ concentrations. Its stationary voltage dependence was however shifted in a depolarizing direction by increasing, and in hyperpolarizing direction by decreasing the extracellular Ca++ concentration. In view of these findings, and of its kinetic properties, the slow K inactivation was classified as a genuine channel gating process. The process of K activation was too fast for a dynamic analysis with the recording technique available. An estimate of its stationary voltage dependence could however be obtained in a voltage range from about -100 to about -40 mV. The experimental observations were utilized in the formulation of a mathematical model describing the kinetic behaviour of IK in the present preparation based on constant field and state transition theories.
mesh_terms: D015761:4-Aminopyridine; D000222:Adaptation, Physiological / Q000187:drug effects; D000631:Aminopyridines / Q000494:pharmacology*; D000818:Animals; D007473:Ion Channels / Q000187:drug effects*; D007700:Kinetics; D008465:Mechanoreceptors / Q000187:drug effects*; D008954:Models, Biological; D008121:Nephropidae; D009474:Neurons / Q000187:drug effects*; D011188:Potassium / Q000378:metabolism*; D013757:Tetraethylammonium Compounds / Q000494:pharmacology*
chemical_list: D000631:Aminopyridines; D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D015761:4-Aminopyridine; D011188:Potassium