[Binding of NR1 plasmid DNA to membrane during replication in Escherichia coli mini-cells].
Studying the replication of NR1 plasmid in E. coli mini-cells it was shown that the character of bond between plasmid DNA and membrane depends on the stage of replication cycle of the plasmid. On initiation the DNA-membrane complex is sensitive to the action of ionic force. In the process of elongation the bond of DNA molecules with the membrane is unstable if exists at all, and can be broken even by the nonionic detergent. At the final stage of replication the newly synthesized molecules form a complex with the membrane structures which is unstable in the presence of 0,5 M NaCl. The destruction of the complex followed by the open cycle of plasmid DNA coming out of it takes place under the action of ionic detergent.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Kharakter sviazi s membranoĭ DNK plazmidy NR1 v protsesse replikatsii v mini-kletkakh Escherichia coli.