Neutrophil migration under agarose in healthy and cytomegalovirus-infected mice.
A technique of measuring directed and random migration of mouse neutrophils was developed to study these neutrophil functions in a standardized small animal population into which specific variables could be introduced. Values in healthy animals were highly reproducible; variations in random and directed migration did not exceed 9%. Mouse neutrophils had significant impairment of directed and random migration at 3, 5, and 8 days after infection with cytomegalovirus. Technical reproducibility and the ability to reflect a disease process make this model a promising one for the laboratory study of neutrophil migration.
D000818:Animals; D002452:Cell Count; D002634:Chemotaxis, Leukocyte; D003470:Culture Media; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005260:Female; D051379:Mice; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D012685:Sepharose
D003470:Culture Media; D012685:Sepharose