Mechanism of interferon action. Inhibition of vesicular stomatitis virus in human amnion U cells by cloned human leukocyte interferon.
The effects of a subsaturating, long treatment (24 h) dose of a highly purified cloned subspecies of human leukocyte interferon (IFN-alpha A) on vesicular stomatitis virus (VSV) primary macromolecular synthesis in tsG41-infected human amnion U cells were examined. IFN-alpha A, under these conditions, was found to inhibit primary VSV protein synthesis ten-fold while producing no detectable effect on the amount or integrity of primary viral message transcripts. There was no selective reduction by IFN-alpha A of the VSV G or M proteins.
D000650:Amnion / Q000382:microbiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D007370:Interferon Type I; D012367:RNA, Viral; D014764:Viral Proteins