[Effect of nicotine on polyphosphoinositides of erythrocytes from spontaneously hypertensive rats].
We have investigated the in vitro action of nicotine (1 . 10(-6)M) on the content of and 32P incorporation into di- and triphosphoinositides of erythrocyte membranes from spontaneously hypertensive rats (SHR) and WKY control rats (both 10 weeks of age). The triphosphoinositide content was found increased after incubation with nicotine for 0.5 min and decreased after incubation for 20 min in both strains. The diphosphoinositide content was decreased after 5 min and much more after 20 min. The specific activity for the phosphate groups of phosphoinositides increased during incubation with nicotine for 0.5 min in rats of both strains. Recovery of preincubation activity values ("normalization") was faster in SHR than in WKY rats. Explanations are given concerning the rate of 32P incorporation into polyphosphoinositides and recovery of cholinergic excitability of erythrocyte membranes.
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006973:Hypertension / Q000097:blood* / Q000235:genetics; D007700:Kinetics; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D009538:Nicotine
Wirkung von Nikotin auf Polyphosphoinositide der Erythrozyten spontan hypertoner Ratten.