Nucleoside 5'-(beta, gamma-peroxytriphosphates).
Procedures for the synthesis, purification, and characterization of beta, gamma-peroxy analogues of the eight common ribo- and deoxyribonucleoside triphosphates have been developed. Although adenosine 5'-(beta, gamma-peroxytriphosphate) was stable to conditions in most biochemical systems, incubation of a solution of the analogue at 100 degrees C led to formation of AMP and ATP, as well as ADP. NAD+ pyrophosphorylase was the only enzyme among 13 tested for which adenosine 5'-(beta, gamma-peroxytriphosphate) was a good substrate, but the analogue was an effective inhibitor for a number of kinases. The peroxy compounds tested inactive with Escherichia coli RNA polymerase and DNA polymerase I, as well as with wheat germ RNA polymerase II.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification; D000818:Animals; D004256:DNA Polymerase I / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007202:Indicators and Reagents; D008099:Liver / Q000201:enzymology; D008156:Luciferases / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D010770:Phosphotransferases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology; D012486:Salmonella typhimurium / Q000201:enzymology; D013329:Structure-Activity Relationship
D007202:Indicators and Reagents; C032451:adenylyl 5'-peroxydiphosphate; D000255:Adenosine Triphosphate; D008156:Luciferases; D010770:Phosphotransferases; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I