Association of benzo[a]pyrene with H1 histone in rat-liver nuclei.
Rat-liver nuclei were incubated with microsomal fraction, NADPH and [3H]benzo[a]pyrene ([3H]BP). From the incubated nuclei, H1 histone was extracted and purified. The purified histone was acid-hydrolyzed; the hydrolysate was filtered on a column of Sephadex LH-20. The 4 radioactive fractions thus obtained by methanol gradient were about 44% of the total radioactivity. By thin-layer chromatography, 1 ninhydrin-positive and fluorescent spot was detected in each of the 2 radioactive fractions. The gas chromatographic and mass spectrometric analyses suggest that 1 of these 2 fractions contains a BP-diol epoxide-lysine conjugate.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000032:analysis* / Q000378:metabolism; D002273:Carcinogens / Q000032:analysis* / Q000378:metabolism; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D002845:Chromatography; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D001580:Benzopyrenes; D002273:Carcinogens; D006657:Histones; D001564:Benzo(a)pyrene; D009249:NADP