title: cAMP synthesis in the rat oocyte.
abstract: cAMP synthesis by the rat oocyte and cumulus-oocyte complex was studied using direct labeling techniques. Cumulus-oocyte complexes synthesized cAMP in response to luteinizing hormone, follicle-stimulating hormone, cholera toxin, and forskolin. However, naked oocytes prepared from cumulus-oocyte complexes by mechanically removing the cumulus cells synthesized cAMP only in response to forskolin and follicle-stimulating hormone; cholera toxin and luteinizing hormone did not stimulate cAMP synthesis. Cholera toxin could augment the response of the oocytes to FSH, indicating an intact, though atypical, adenylate cyclase system. Forskolin was found to inhibit the onset of oocyte maturation in both cumulus-oocyte complexes and naked oocytes. The implications of these findings for the relationship between cAMP synthesis and oocyte maturation in the rat are discussed.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D005576:Colforsin; D000242:Cyclic AMP / Q000096:biosynthesis*; D004224:Diterpenes / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007700:Kinetics; D007986:Luteinizing Hormone / Q000494:pharmacology; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism*; D009866:Oogenesis / Q000187:drug effects; D051381:Rats
chemical_list: D004224:Diterpenes; D005576:Colforsin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D000262:Adenylyl Cyclases