Isolation and characterization of an EBV superinfection-enhanced, host-cell-coded, 53,000-dalton protein.
Microsomal membrane fraction proteins with enhanced synthesis after P3HR-1 Epstein-Barr virus (EBV) superinfection of Raji cells were identified with [35S]-methionine labeling and SDS-PAGE. One 53,000-dalton protein, which was found in both the microsomal membrane and cytosol fractions, was purified by ion-exchange chromatography, and specific rabbit antisera were prepared to it. This protein was found to be present in Raji cells, but its expression was enhanced after P3HR-1 EBV superinfection. It was more abundant in the cytosol than in the microsomal membrane fraction of the cell, and its synthesis was not affected by treatment of the cells with phosphonoacetic acid. It was present in several EBV-genome-negative cell lines and in activated B lymphocytes and consequently represents a host-cell-coded protein which is enhanced by EBV superinfection or by lymphocyte activation.
D002460:Cell Line; D003600:Cytosol / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007425:Intracellular Membranes / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis*; D008861:Microsomes / Q000032:analysis; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins