Testicular responsiveness to hCG of the ovine fetus in the last third of gestation.
The in vivo and in vitro testicular responsiveness to hCG of hemicastrated lamb fetuses 95-99, 110-118 and 130-141 days of gestational age was studied. Basal plasma testosterone (T) levels were similar at all ages (less than 0.25 ng/ml), while the mean testicular concentrations of dehydroepiandrosterone sulfate (DHA-S), 17 alpha-hydroxyprogesterone (17-OHP) and T were higher in 95- to 99-day-fold fetuses. Plasma T levels and the concentration of T, DHA-S, 17-OHP, androstenedione (A) and cyclic adenosine 3'5'-monophosphate (cAMP) were increased by hCG in the hemicastrated animal at all ages. cAMP and T production by enriched preparations of dispersed interstitial cells from control testes was increased by hCG in all groups. In fetuses pretreated with hCG in vivo the addition of hCG in vitro failed to modify cAMP and T production. 100 micrograms of LHRH to a 130-day-old fetus increased plasma LH and T levels. From these experiments, it is suggested that the low plasma LH and T levels found throughout the last trimester of fetal life reflect a relative lack of endogenous LHRH synthesis and/or release, rather than reduced testicular steroidogenic capacity.
D019326:17-alpha-Hydroxyprogesterone; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis; D003687:Dehydroepiandrosterone / Q000032:analysis; D005260:Female; D005865:Gestational Age; D006801:Humans; D006908:Hydroxyprogesterones / Q000032:analysis; D008297:Male; D011247:Pregnancy; D012756:Sheep / Q000196:embryology*; D013737:Testis / Q000032:analysis / Q000187:drug effects* / Q000196:embryology; D013739:Testosterone / Q000032:analysis / Q000097:blood
D006063:Chorionic Gonadotropin; D006908:Hydroxyprogesterones; D013739:Testosterone; D003687:Dehydroepiandrosterone; D019326:17-alpha-Hydroxyprogesterone; D000242:Cyclic AMP