title: A rho-dependent transcription termination signal in bacteriophage f1.
abstract: The bacteriophage f1 intergenic region distal to gene IV encodes a rho-dependent transcription termination signal. Terminator function in vivo and in vitro is dependent upon active Escherichia coli rho protein, although the RNA 3' ends detected in vivo differ from those seen in vitro. The minimal sequence required for terminator function in a heterologous plasmid system encompasses approximately 100 nucleotides distal to gene IV, which can be drawn as a large hairpin structure. The in vivo rho-dependent 3' end occurs within this sequence, while the in vitro rho-dependent 3' ends occur just distal to it. In vivo in a rho mutant host, f1 transcripts pass through the rho-dependent sites and stop within a sequence of high potential secondary structure near the f1 origin of DNA replication. This sequence alone causes transcription termination in the heterologous plasmid system in vivo. In vitro in the absence of rho protein, transcription does not terminate within this sequence. The RNA 3' ends detected in these studies do not occur within A + T-rich sequences.
mesh_terms: D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000032:analysis; D012234:Rho Factor / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D012234:Rho Factor; D014157:Transcription Factors; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases