[Cloning of the gene controlling catabolite repression with the participation of cyclic adenosine monophosphate in Escherichia coli K-12].
The crp gene coding for cyclic adenosine monophosphate receptor protein has been cloned on the vehicle pBR325 using restriction endonuclease PstI and the recipient strain C600 crp. The pCAP2 hybrid plasmid obtained has a molecular weight 7.0 MD and in the pBR325 with the insertion into a PstI site. Bacterial clones carrying pCAP2 restore Crp+ phenotype, as judged by the capacity of bacteria for utilization of various carbohydrates and by the activity of catabolite sensitive enzymes.
D003001:Cloning, Molecular / Q000379:methods; D000242:Cyclic AMP / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005784:Gene Amplification; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011953:Receptors, Cyclic AMP / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D004269:DNA, Bacterial; D011953:Receptors, Cyclic AMP; D012097:Repressor Proteins; D014157:Transcription Factors; D000242:Cyclic AMP
Klonirovanie gena, kontroliruiushchego katabolitnuiu repressiiu s uchastiem tsiklicheskogo adenozinmonofosfata u Escherichia coli K-12.