Transport of potassium, amino acids, and glucose in cells transformed by Rous sarcoma virus.
Transport rates of a number of nutrients and ions have been surveyed in chicken embryo fibroblasts that were density inhibited, growing exponentially, or transformed by Rous sarcoma virus. All the transport systems examined displayed changes associated with changes in growth rate. The rate of ouabain-sensitive potassium transport declined in density-inhibited cells, and increased rapidly in response to serum stimulation. This transport system was regulated both by changes in the activity of the transporters and by the number of transporters in the cell membrane. The rate of transport of the amino acid analog alpha-aminoisobutyric acid declined when cells became density inhibited, but also showed alterations in regulation that were associated with malignant transformation. The rate of glucose transport displayed both growth state-related and transformation-specific changes. The increased rate of glucose transport seen in transformed cells is due to an increase in the number of glucose transporters in the cell membrane. Increased glucose transport is necessary for subsequent changes in glycolysis, and temporally precedes some of the changes in activity of glycolytic enzymes.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001692:Biological Transport; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D005347:Fibroblasts / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins; D011188:Potassium / Q000378:metabolism*
D000596:Amino Acids; D002352:Carrier Proteins; D009004:Monosaccharide Transport Proteins; D005947:Glucose; D011188:Potassium