Adenosine diphosphate ribosyltransferase and protein acceptors associated with cytoplasmic free messenger ribonucleoprotein particles.
ADP-ribosyltransferase activity has been characterized in free messenger ribonucleoprotein particles (mRNP) from mouse plasmacytoma cells. This enzymatic activity appears to be associated with the free mRNP and not due to nuclear contamination. The enzyme activity is not stimulated by added DNA or histone H1 and represents 34 per cent of the total cellular ADP-ribosyltransferase activity while the DNA contamination in free mRNP is less than 4 per cent of the total cellular DNA. Moreover, the ADP-ribosyltransferase specific activity per mg of DNA is about 75-fold higher in free mRNP than in the nuclei. During CsCl gradient centrifugation of the cytoplasmic fraction, the ADP-ribosylated material separates out at a buoyant density similar to that of free mRNP. This ADP-ribosyltransferase activity is inhibited by thymidine, nicotinamide and 3-aminobenzamide, while it is highly stimulated by exogenous pancreatic RNase. The in vitro synthesized acid insoluble material is rendered partly soluble by treatment by a proteolytic enzyme or by snake venom phosphodiesterase resulting in phosphoribosyl-AMP formation: the pancreatic RNase does not solubilize this material. Several ADP-ribosylated proteins are detected by lithium dodecylsulfate gel electrophoresis. Such an ADP-ribosyltransferase activity has also been detected in free mRNP from rat liver. It is suggested that this ADP-ribosylation of specific free mRNP proteins may be associated with free mRNP structure and/or with some chemical covalent type of modification rendering mRNA available for translation.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D006867:Hydrolases / Q000494:pharmacology; D051379:Mice; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010954:Plasmacytoma / Q000201:enzymology*; D011065:Poly(ADP-ribose) Polymerases; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D012261:Ribonucleoproteins / Q000378:metabolism*
D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D000246:Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases; D006867:Hydrolases; D012259:Ribonuclease, Pancreatic