title: Solubilization of brain benzodiazepine receptors with a zwitterionic detergent: optimal preservation of their functional interaction with the GABA receptors.
abstract: Rat brain benzodiazepine receptors have been solubilized by means of the zwitterionic detergent CHAPS under conditions in which the GABA stimulation of [3H]flunitrazepam binding to the benzodiazepine receptors is maintained intact. This stimulation is partially or totally abolished when using other conventional detergents.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002793:Cholic Acids; D005445:Flunitrazepam / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism*; D011963:Receptors, GABA-A; D012995:Solubility; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D002793:Cholic Acids; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate