Variation of virus replication in primary bovine kidney cell cultures derived from 68 three-day-old calves.
Primary kidney cell cultures were prepared from 68 three-day-old calves. Complete monolayers of these cultures were infected separately with viral diarrhea, infectious bovine rhinotracheitis, parainfluenza, and adenovirus 7 viruses. The yield of virus from all infected cultures was calculated by plaque titer assay after 2 to 4 days' incubation. The variation of virus yield was substantial between individual cultures.
D000256:Adenoviridae / Q000254:growth & development*; D000818:Animals; D002417:Cattle / Q000235:genetics; D046508:Culture Techniques; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development*; D004032:Diet; D005260:Female; D007242:Herpesvirus 1, Bovine / Q000254:growth & development*; D007668:Kidney; D008297:Male; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development*; D010576:Pestivirus / Q000254:growth & development*; D018108:Respirovirus / Q000254:growth & development*; D014779:Virus Replication