Separation and quantitation of leukotrienes by reversed-phase high-performance liquid chromatography.
A rapid and sensitive reversed-phase HPLC procedure is reported which allows the simultaneous separation and quantitation of LTC4, 11t-LTC4, LTD4, LTB4, 12epi,6t,8c-LTB4, 6t-LTB4 + 12epi,6t-LTB4, two trihydroxy-eicosatetraenoic acids tentatively identified as 20-OH-LTB4 and 20-OH,12epi,6t,8c-LTB4 and several not yet identified 15-series leukotrienes produced by the cytosol of porcine polymorphonuclear leukocytes.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007975:Leukotriene B4 / Q000302:isolation & purification*; D009504:Neutrophils / Q000378:metabolism; D013189:SRS-A / Q000302:isolation & purification*; D013552:Swine
D001095:Arachidonic Acids; D013189:SRS-A; D007975:Leukotriene B4; D016718:Arachidonic Acid