title: The mechanism of antibody inhibition of proton pumping by cytochrome oxidase vesicles.
abstract: Antibodies previously shown to inhibit vectorial proton translocation through cytochrome oxidase vesicles were converted to F(ab')2 and Fab'. Neither fragment inhibited proton pumping, although binding capacity was present. However, when a surrogate Fc was added to F(ab')2, inhibition of proton translocation was restored; indicating that the inhibition is due to steric hindrance. These results provide insight into mechanisms of energy transduction by oxidase.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002417:Cattle; D003576:Electron Transport Complex IV / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D007140:Immunoglobulin Fab Fragments / Q000502:physiology*; D007074:Immunoglobulin G / Q000502:physiology*; D009206:Myocardium / Q000201:enzymology; D010446:Peptide Fragments / Q000276:immunology; D011522:Protons; D011817:Rabbits
chemical_list: D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011522:Protons; D003576:Electron Transport Complex IV