Energetics of calcium efflux from cells of Escherichia coli.
Intact cells of a H+-translocating ATPase-deficient strain of Escherichia coli were starved of endogenous energy reserves and passively loaded with 45CaCl2. Energy-dependent efflux of calcium was observed upon addition of glucose or respiratory substrates. Addition of cyanide or uncouplers prevented efflux. It is concluded that calcium efflux in intact cells is coupled to the proton motive force via secondary calcium-proton exchange.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D004734:Energy Metabolism; D004926:Escherichia coli / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D011522:Protons; D012966:Sodium Cyanide / Q000494:pharmacology; D013696:Temperature
D011522:Protons; D006859:Hydrogen; D000255:Adenosine Triphosphate; D012966:Sodium Cyanide; D002118:Calcium