The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus.
We have determined the nucleotide sequence of a cloned 1710-bp segment of Haemophilus haemolyticus DNA which contains the HhaII restriction (r) and modification (m) genes. The m gene is 513 bp in length and the rgene is 681 bp in length. Both are in the same reading frame, being separated by a 21-bp region. A ribosome-binding site is identified in front of each gene, but no Haemophilus promoter is apparent on the cloned fragment. Transcription originates from a plasmid promotor and proceeds in the direction m to r.
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005796:Genes; D005798:Genes, Bacterial; D006190:Haemophilus / Q000201:enzymology / Q000235:genetics*
D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases