Structural relationship between the chicken DNA locus, proto-fps, and the transforming gene of Fujinami sarcoma virus, delta gag-fps.
The avian Fujinami sarcoma virus (FSV) contains a hybrid transforming gene (delta gag-fps) with a 5' 1.3-kb portion derived from the gag gene of avian retroviruses and a 3' 2.8-kb portion (fps) derived from a cellular prototype. A lambda recombinant DNA clone carrying fps sequences within a 16-kb insert of cellular DNA, termed lambda proto-fps clone 12, has been selected from a chicken DNA library for comparison with the viral onc gene. Mapping of endonuclease-resistant proto-fps DNA fragments and hybridization with cloned viral DNA located FSV-related sequences at the 3' end of the insert within a region of about 4.25 kb. Alignment of endonuclease-resistant proto-fps and viral DNA fragments relative to common RNase T1-resistant oligonucleotide sequences of viral RNA, identified by fingerprinting DNA-RNA hybrids, indicated: (i) that proto-fps is colinear with viral fps but is interrupted by 1.75 kb of scattered sequences unrelated to viral fps; (ii) that among the nine endonuclease sites compared, proto-fps and viral fps share one PvuII, one BamHI, and possibly a Kpn1 site at homologous locations, and that they each have unique endonuclease sites and common sites at unique locations; (iii) that within 12 kb upstream from the 5' boundary of overlap with viral fps, proto-fps lacks gag-related sequences; and (iv) that proto-fps clone 12, like several others isolated by us, lacks at the 3' end an equivalent of the 3' 10 to 20% of viral fps. The eight endonuclease site-map coordinates of proto-fps and viral DNA also divided 44 fps oligonucleotides of viral RNA into 9 map segments. We conclude that the onc gene of FSV differs from proto-fps in delta gag and in multiple point mutations, compatible with a transforming function for the viral gene and a normal function for the cellular sequence homolog. Since proto-fps is unrelated to essential virion genes, the onc gene of FSV must have originated from cellular proto-fps by rare, illegitimate recombination.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002645:Chickens / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012367:RNA, Viral; D011995:Recombination, Genetic
D012367:RNA, Viral; D004262:DNA Restriction Enzymes