Detergent extraction from rat synaptosomal plasma membranes reveals difference in mu and delta opioid receptor binding.
Rat synaptosomal plasma membranes were extracted with a detergent (CHAPS, a zwitterionic derivative of cholic acid). mu and delta opioid receptor binding and adenylate cyclase activities were tested in the intact membranes and in the supernatants from detergent treated membranes. The 6000 X g/8 min. supernatant contained mu receptor binding equal to 33% of the mu receptor binding measured in the untreated membranes. When the detergent treated membranes were sedimented at (50,000 X g/10 min.), 23% of the mu receptor binding was recovered in the supernatant. After a 100,000 X g/30 min. centrifugation the supernatant contained 10% of the mu receptor binding when compared to untreated membranes. Of the delta receptor binding found in intact membranes, 10% or less was recovered in the 3 supernatants described above. Furthermore, the mu and delta receptor binding were distributed differently among particles in the supernatants. This indicates differences in the chemical properties of the mu and delta opioid receptors. Adenylate cyclase assays showed that the G/F site of this enzyme complex was inactivated in the supernatants from detergent treated membranes parallel to the delta receptor binding decrease. However, the catalytic part of adenylate cyclase was present in the supernatants and seemed resistant to the detergent.
D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D003902:Detergents / Q000494:pharmacology*; D004745:Enkephalins / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009270:Naloxone / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats; D011957:Receptors, Opioid / Q000032:analysis* / Q000378:metabolism; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology*; D013574:Synaptosomes / Q000032:analysis*
D003902:Detergents; D004745:Enkephalins; D011506:Proteins; D011957:Receptors, Opioid; D013501:Surface-Active Agents; D009270:Naloxone; D000262:Adenylyl Cyclases