title: Independent expression of common and private tumor-specific cell surface antigens in somatic cell hybrids between Rous sarcoma virus-transformed and normal cells.
abstract: Somatic cell hybrids were prepared by fusion of a Rous sarcoma virus (RSV)-induced mouse tumor (CSA1M cl-11) with a Chinese hamster embryo lung fibroblast line (Wg3h X AGr X Ouar). Some hybrid clones showed rapid anchorage-independent growth and intracellular production of RSV-pp60src. Other hybrid clones showed slow anchorage-dependent growth and no production of such a protein. The former expressed both a common tumor-specific cell surface antigen(s) (TSSA) shared with other RSV-induced tumors and a private TSSA not shared with any other tumors; however, the latter lost the common TSSA and expressed only the private TSSA. These results suggest that the common and private TSSA on CSA1M cl-11 cells are independent genetic traits and only the common TSSA is coordinated with other expressions of the RSV src gene phenotype.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001358:Avian Sarcoma Viruses; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006822:Hybrid Cells / Q000276:immunology*; D015688:Oncogene Protein pp60(v-src); D010641:Phenotype; D011247:Pregnancy; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014764:Viral Proteins; D015688:Oncogene Protein pp60(v-src)