title: Structure elucidation by one- and two-dimensional 360- and 500-MHz 1H NMR of the oligosaccharide units of two glycoproteins isolated from alveoli of patients with alveolar proteinosis.
abstract: The structure of the oligosaccharide units of the glycoproteins of Mr 36,000 and 62,000 isolated from alveoli of patients with alveolar proteinosis have been determined by one- and two-dimensional 1H NMR spectroscopy at 500 and 360 MHz. Bi-, tri-, and tetraantennary glycans of N-acetyllactosaminic type have been found in high percentage. They are 1----6 monofucosylated and fully sialylated, the ratio NeuAc(2----3)/NeuAc X (2----6) increasing with increasing degree of branching.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification*; D011522:Protons; D011649:Pulmonary Alveolar Proteinosis / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000032:analysis*
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D011522:Protons