title: Ultrastructural characterization of herpes simplex virus type 1 (strain 17) temperature-sensitive mutants.
abstract: We report the ultrastructural features associated with wild-type and temperature-sensitive (ts) mutant infection at non-permissive temperature for each of 18 herpes simplex virus type 1 (HSV-1) (strain 17) ts mutants. The mutants were classified by their ability to induce nucleocapsid-related structures: class I failed to induce any, class II induced empty and partially filled structures while class III induced all of the identified structures including those containing an electron-dense core. The time when expression of the ts lesion blocked virus replication was estimated for most mutants; this allows both mutant gene expression and the resulting ultrastructural features to be correlated with the sequence of virus replicative events. Three ts mutant-triggered features not previously described in HSV-1-infected cells were also recognized: a modification of rough endoplasmic reticulum, intranuclear accumulation of enveloped virus particles and cytoplasmic accumulation of novel doughnut-shaped particles having a concentric double ring appearance in thin section.
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003593:Cytoplasm / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D007668:Kidney; D008854:Microscopy, Electron; D009154:Mutation; D018139:Simplexvirus / Q000235:genetics / Q000648:ultrastructure*; D013045:Species Specificity; D013696:Temperature