Fine structural aspects of phagocytotic activity in inverted follicle cells of cultured porcine thyroids.
Inversion of thyroid follicles took place when they were isolated by collagenase and trypsin and cultured in suspension in Eagle's medium supplemented with 10% fetal calf serum without TSH. The apical surface facing the culture medium contained numerous microvilli and a central cilium, while the luminal surface became flattened. Phagocytotic activity by pseudopods was promoted after addition of TSH to the culture medium. When the inverted follicles were incubated in culture medium containing TSH (50 mU/ml) and human red blood cells, or TSH and polystyrene latex beads (2.02 micron in diameter) for 1-3 h, numerous red blood cells or latex beads respectively were observed to be taken up by the epithelial follicle cells by scanning electron microscopy, as well as conventional thin-section electron microscopy. These results show that the apical surface (culture medium side) of the epithelial cell of the cultured thyroid follicle whose polarity is reversed phagocytoses red blood cells and latex beads.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007840:Latex; D003012:Microbial Collagenase; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D013552:Swine; D013961:Thyroid Gland / Q000502:physiology / Q000648:ultrastructure*; D013972:Thyrotropin; D014357:Trypsin
D003470:Culture Media; D007840:Latex; D013972:Thyrotropin; D014357:Trypsin; D003012:Microbial Collagenase