ESR analysis of the FAD in bovine liver monoamine oxidase.
Two hydrazine spin labels, 1-oxyl-2,2,5,5-tetramethylpyrroline-3-carbonyl ethyl hydrazine and 1-oxyl-2,2,6,6-tetramethylpiperidino-4-hydrazine, were synthesized as probes of the FAD binding site of monoamine oxidase. The reporter nitroxide moiety showed an ESR spectrum classified as partially immobilized which is indicative of FAD near the surface of the enzyme. Attempts to pick up flavin semiquinone or free radical intermediates during substrate oxidation with the spin traps 5,5-dimethyl-1-pyrroline-1-oxidase and phenyl-t-butylnitrone were not successful.
D000818:Animals; D002417:Cattle; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005182:Flavin-Adenine Dinucleotide; D008099:Liver / Q000201:enzymology*; D008995:Monoamine Oxidase; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; D005182:Flavin-Adenine Dinucleotide; C040218:2,2,5,5-tetramethylpyrroline-1-oxyl-3-carboxy ethylhydrazine; C040219:2,2,6,6-tetramethylpiperidino-1-oxyl-4-hydrazine; D008995:Monoamine Oxidase