A comparison of avian and rodent S-9 liver homogenates in the Ames test.
Avian S-9 liver homogenates were obtained from phenobarbital, 3-methylcholanthrene (3-MC), polychlorinated biphenyl (Aroclor 1254) induced and control chickens. These homogenates were tested for their capacity to metabolically activate benzo[a]pyrene (B[a]P) and cyclo-phosphamide in the Salmonella typhimurium assay. Liver homogenates from 3-MC, Aroclor 1254 and phenobarbital induced chickens were more active in converting B[a]P to its ultimate mutagenic form than was S-9 obtained from Aroclor 1254 induced rats. However, Aroclor 1254 induced rat S-9 was superior to Aroclor 1254 induced avian S-9 in metabolically activating cyclophosphamide.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism; D001711:Biotransformation; D002645:Chickens; D003520:Cyclophosphamide / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000032:analysis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D001580:Benzopyrenes; D009153:Mutagens; D001564:Benzo(a)pyrene; D003520:Cyclophosphamide; D006899:Mixed Function Oxygenases