A gel filtration method to test the efficiency of virucidal disinfectants.
We have described a gel filtration technique for assay of the virucidal potency of disinfectants. It allows a complete separation of disinfectant from the virus after contact, thus preventing chemical cytotoxicity. Very low residual infectious activity can be measured. There is no need for a high viral titer antigen.
D000956:Antigens, Viral / Q000592:standards; D000998:Antiviral Agents / Q000494:pharmacology*; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods; D004202:Disinfectants / Q000494:pharmacology*; D011051:Poliomyelitis / Q000517:prevention & control*; D017955:Poliovirus / Q000187:drug effects / Q000254:growth & development; D014615:Vaccinia / Q000517:prevention & control*; D014616:Vaccinia virus / Q000187:drug effects / Q000254:growth & development; D014775:Virus Activation / Q000187:drug effects
D000956:Antigens, Viral; D000998:Antiviral Agents; D004202:Disinfectants