Regulated expression of a gene important for replication of plasmid F in E. coli.
Fusions between the gene encoding the E protein of the IncFI plasmid F and the lac genes were constructed. Analysis of the expression of beta-galactosidase from these fusions shows that the promoter for the E protein gene is located between the incB region and the structural gene for the E protein. Near this promoter is a regulatory site on which a negative control element acts. Most likely the E protein itself acts as a repressor of E gene expression and thus autoregulates its own expression. No other gene products seem to affect the expression of the E protein gene.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005144:F Factor; D005786:Gene Expression Regulation; D009876:Operon; D012097:Repressor Proteins / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; D001616:beta-Galactosidase