Effect of (E)-5-(2-bromovinyl)-2'-deoxyuridine on several parameters of Epstein-Barr virus infection.
The selective and potent anti-herpesvirus drug, (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdU), has been examined for its inhibitory effects on several parameters of Epstein-Barr virus (EBV) infection in the lymphoblastoid cell lines Raji, P3HR-1, B-95-8 and P3 hybrid cells (a human embryo oropharyngeal cell line fused with a nasopharyngeal carcinoma cell line). At a dosage of 0.03 to 0.1 mM, BVdU caused a marked inhibition of (i) spontaneous viral capsid antigen (VCA) expression in B-95-8 and P3 hybrid cells, (ii) VCA expression and DNA synthesis in B-95-8 cells induced with croton oil and n-butyrate, (iii) early antigen (EA) expression and DNA synthesis in Raji cells superinfected with EBV, and (iv) VCA expression and DNA synthesis in B-95-8 cells superinfected with EBV. In its inhibitory effects on these various parameters of EBV infection, BVdU appears to be comparable to acyclovir [9-(2-hydroxyethoxymethyl)guanine], another selective anti-herpesvirus drug which has been previously recognized as an effective inhibitor of EBV replication.
D000212:Acyclovir / Q000627:therapeutic use; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D001087:Arabinonucleosides / Q000627:therapeutic use; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002051:Burkitt Lymphoma / Q000188:drug therapy* / Q000382:microbiology; D002143:Callitrichinae; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D006566:Herpesviridae Infections / Q000188:drug therapy* / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000187:drug effects; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects / Q000382:microbiology; D066298:In Vitro Techniques; D013936:Thymidine / Q000031:analogs & derivatives / Q000627:therapeutic use; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D001087:Arabinonucleosides; C020235:brivudine; D001973:Bromodeoxyuridine; C010032:thymine arabinoside; D013936:Thymidine; D000212:Acyclovir