beta-Adrenergic receptor regulation of N-linked protein glycosylation in rat parotid acinar cells.
We have investigated the relationship between beta-adrenergic receptor stimulation and protein glycosylation and secretion in rat parotid gland cells in vitro. The potent beta-adrenergic agonist (-)-isoproterenol increases [3H]mannose incorporation into newly synthesized glycoproteins. This effect is enhanced if cells are first preincubated with dolichyl phosphate and is not observed after muscarinic-cholinergic or alpha-adrenergic stimulation of cells. The increase in [3H]mannose incorporation is abolished by incubation of cells with tunicamycin, suggesting that the glycosylation events being studied involved asparagine-linked oligosaccharides. The extent of increase in glycosylation is dependent on the concentration of (-)-isoproterenol to which cells are exposed. (+/-)-Propanolol totally abolishes the (-)-isoproterenol-induced increase in [3H]mannose incorporation, in a manner similar to its effects on exocrine secretion. Our findings suggest that beta-adrenergic receptor activation has a profound influence on N-linked protein glycosylation in rat parotid cells in addition to eliciting exocrine protein release.
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002250:Carbon Radioisotopes; D004288:Dolichol Phosphates / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006023:Glycoproteins / Q000235:genetics*; D007545:Isoproterenol / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000378:metabolism; D010306:Parotid Gland / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D004288:Dolichol Phosphates; D006023:Glycoproteins; D011943:Receptors, Adrenergic, beta; D014316:Tritium; C028306:dolichol monophosphate; D002217:Carbachol; D007930:Leucine; D007545:Isoproterenol; D008358:Mannose; D004837:Epinephrine