The induction of resistant hepatocytes during initiation of liver carcinogenesis with chemicals in rats fed a choline deficient methionine low diet.
Feeding a choline deficient methionine low (CLD) diet for two weeks can substitute for partial hepatectomy in the genesis with benzo[a]pyrene (B[a]P) or 1,2-dimethylhydrazine (1,2-DMH) of resistant hepatocytes that can be selected with dietary 2-acetylaminofluorene plus partial hepatectomy. With single doses of B[a]P or 1,2-DMH during the feeding of the CLD diet for 3 weeks, the number of foci of hepatocytes positive for gamma-glutamyl transferase is approximately the same as with the same dose of each carcinogen given after partial hepatectomy. DL-ethionine was now found to be positive when given as a single dose to animals on the CLD diet. Feeding ethionine for 6 or 12 weeks in a similar choline lipotrope deficient diet also induced a significant increase in foci of enzyme altered hepatocytes. The results indicate that dietary imbalance can have an important influence on initiation of chemical carcinogenesis.
D019813:1,2-Dimethylhydrazine; D015073:2-Acetylaminofluorene / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D002796:Choline Deficiency / Q000503:physiopathology*; D004127:Dimethylhydrazines / Q000633:toxicity; D006498:Hepatectomy; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology* / Q000517:prevention & control; D008297:Male; D008715:Methionine / Q000172:deficiency*; D051381:Rats; D011916:Rats, Inbred F344
D001580:Benzopyrenes; D002273:Carcinogens; D004127:Dimethylhydrazines; D001564:Benzo(a)pyrene; D015073:2-Acetylaminofluorene; D008715:Methionine; D019813:1,2-Dimethylhydrazine