A helix-destabilising protein from herpes simplex virus type I infected cells which specifically stimulates the virus induced DNA polymerase activity in vitro.
We have isolated a protein fraction from HSV-1 infected cells which binds specifically to single-stranded DNA, facilitates a lowering of the melting temperature of poly[d(A-T)] and specifically stimulates the activity of the homologous virus-induced DNA-dependent DNA polymerase in vitro. These are major characteristics of a helix-destabilising protein, exemplified by the prokaryotic gene 32 protein.
D004268:DNA-Binding Proteins / Q000502:physiology*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004789:Enzyme Activation; D011067:Poly dA-dT / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics*; D014779:Virus Replication
D004268:DNA-Binding Proteins; D011067:Poly dA-dT; D004259:DNA-Directed DNA Polymerase