In organello and in vitro phosphorylation of chloroplast ribosomal proteins.
Two chloroplast ribosomal proteins are phosphorylated in isolated chloroplast. One in the large subunit (L18) and one in the small subunit ( LS31 ). The phosphorylation is light dependent and occurs on a serine residue for both ribosomal proteins. These two proteins and other chloroplast ribosomal proteins are also phosphorylated in vitro using [gamma 32P]-ATP and a cAMP -dependent or a cAMP - independent protein kinase. The existence of a protein-kinase bound to chloroplast 70S ribosomes is also demonstrated, the enzyme is able to phosphorylate almost every chloroplast ribosomal protein.
D001345:Autoradiography; D047388:Casein Kinases; D002736:Chloroplasts / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology; D004586:Electrophoresis; D008027:Light; D010766:Phosphorylation; D010777:Photochemistry; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants; D011494:Protein Kinases / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*
D010940:Plant Proteins; D012269:Ribosomal Proteins; D000242:Cyclic AMP; D011494:Protein Kinases; D047388:Casein Kinases