Estradiol benzoate induced changes in protein synthesis and lysosomal hydrolases in the pituitary of male rats.
In vitro protein synthesis, lysosomal hydrolases activity and peroxidase activity in the anterior pituitary were estimated in adult male rats treated with 50 micrograms of estradiol benzoate (EB) for 1 day or 7 days. Pituitary protein synthesis, protein and RNA content increased after 7 days. A significant increase in total and membrane-bound acid phosphatase was noted after 1 day or 7 days of EB treatment whereas total beta-glucuronidase activity decreased in both 1 and 7 day group. Cathepsin activity increased after 7 days and pituitary peroxidase system did not change by EB treatment. These findings suggest that immediate change in the enzyme milieu may be one of the first reactions by which EB expresses its feedback control.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009195:Peroxidase; D010544:Peroxidases / Q000378:metabolism; D010902:Pituitary Gland / Q000187:drug effects*; D014176:Protein Biosynthesis; D051381:Rats
D007527:Isoenzymes; D004958:Estradiol; D010544:Peroxidases; D009195:Peroxidase; D000135:Acid Phosphatase; D005966:Glucuronidase; D002403:Cathepsins