Family of middle repetitive DNA sequences in the mouse genome with structural features of solitary retroviral long terminal repeats.
Screening of a 129/J mouse genomic library under nonstringent hybridization conditions with a xenotropic virus-like long terminal repeat (LTR) probe revealed a family of sequences resembling insertion elements (IS) with structural features of solitary retroviral LTRs; these are called LTR-IS. They are interspersed among variable flanking regions of mouse DNA and lack any viral structural genes. LTR-IS elements start and end with 11-base-pair inverted repeats and contain signals implicated in RNA polymerase II transcriptional regulation: C-C-A-A-T, T-A-T-A-A-A, and A-A-T-A-A-A. The members of the family are homologous, but not identical, approximately equal to 500-base-pair-long elements with 4-base-pair target-site duplications on both sites of the element. There are 500 LTR-IS per mouse haploid genome.
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis; D005796:Genes; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*
D004274:DNA, Recombinant; D004247:DNA; D004262:DNA Restriction Enzymes