Effect of dietary corn bran and autohydrolyzed lignin on 3,2'-dimethyl-4-aminobiphenyl-induced intestinal carcinogenesis in male F344 rats.
The effect of dietary corn bran and autohydrolyzed lignin on 3,2'-dimethyl-4-aminobiphenyl (DMAB)-induced intestinal carcinogenesis was studied in male inbred F344 rats. Groups of weanling rats were fed semipurified diets containing 15% corn bran or 7.5% lignin or a semipurified diet without these fibers (control diet). At 7 weeks of age, all animals, except vehicle-treated controls, were given sc injections of 50 mg DMAB/kg body weight/week for 20 weeks. All animals were autopsied 20 weeks after the last injection of DMAB. The incidence of colon tumors (percentage of animals with tumors) and colon tumor multiplicity (tumors/animal) were increased in rats fed the corn bran diet as compared to the tumor incidence and multiplicity in rats fed the control diet. The incidence of small intestinal tumors was slightly lower in rats fed the corn bran diet as compared to the incidence in rats fed the control diet. The concentrations (mg/g dry feces) of fecal deoxycholic acid and total bile acids and the daily output of fecal deoxycholic acid, lithocholic acid, hyodeoxycholic acid, and total bile acids were increased in rats fed the corn bran diet as compared to the concentrations and daily output in rats fed the control diet. The incidence and multiplicity of small intestinal tumors as well as the number of colon adenocarcinomas per tumor-bearing animal were lower in animals fed the lignin diet than in those fed the control diet. Lignin had no effect on the concentrations of fecal bile acids, but the daily output of total bile acids was increased in animals fed the lignin diet as compared to the daily output in rats fed the control diet. This study thus indicates that the protection against colon cancer depends on the type of fiber.
D000611:Aminobiphenyl Compounds; D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity*; D002784:Cholesterol / Q000097:blood; D003110:Colonic Neoplasms / Q000139:chemically induced; D004043:Dietary Fiber / Q000494:pharmacology*; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000633:toxicity*; D005243:Feces / Q000032:analysis; D006358:Hot Temperature; D007414:Intestinal Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control; D008031:Lignin / Q000494:pharmacology*; D008297:Male; D009374:Neoplasms, Experimental / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D003313:Zea mays
D000611:Aminobiphenyl Compounds; D000814:Aniline Compounds; D001647:Bile Acids and Salts; D002273:Carcinogens; D004043:Dietary Fiber; C001042:2',3-dimethyl-4-aminobiphenyl; D008031:Lignin; D002784:Cholesterol; D004159:Diphenylamine