Establishment of spontaneously outgrowing lymphoblastoid cell lines with Cyclosporin A.
To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cells, either at the onset of cultivation or after 3 weeks. The highest frequency of spontaneous outgrowth of EBV-transformed cells was obtained in unseparated cultures when the addition of CSA was delayed. The presence of unimpaired T cells at the onset of the culture period appeared to have a favourable effect on the spontaneous outgrowth of EBV-carrying lymphoblastoid cell lines.
D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes; D002460:Cell Line; D002472:Cell Transformation, Viral; D003524:Cyclosporins / Q000494:pharmacology*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D003524:Cyclosporins; D019309:Epstein-Barr Virus Nuclear Antigens