A technical note on the histochemical demonstration of G6Pase activity.
Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.
D000818:Animals; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates; D006651:Histocytochemistry / Q000379:methods*; D007854:Lead; D008099:Liver / Q000201:enzymology*; D008298:Maleates; D009566:Nitrates; D051381:Rats; D014325:Tromethamine
D005958:Glucosephosphates; D008298:Maleates; D009566:Nitrates; D014325:Tromethamine; D007854:Lead; D019298:Glucose-6-Phosphate; C017461:lead nitrate; C030272:maleic acid; D005952:Glucose-6-Phosphatase