title: HMG17 protein facilitates the DNA catenation reaction catalyzed by DNA topoisomerases.
abstract: HMG17 protein is shown to greatly facilitate the catention of double-stranded DNA rings catalyzed by DNA topoisomerases. Even at low DNA concentrations such that catenanes are not observable in the absence of HMG17, the addition of the protein promotes the catenation of greater than 95% of the input DNA into networks that do not enter the gel upon electrophoresis. Electron microscopy and restriction enzyme cleavage experiments indicate that these networks are large structures containing many catenated DNA rings. The HMG17-promoted DNA network formation has been observed with calf thymus type II DNA topoisomerase and the type I topoisomerases of Escherichia coli, Micrococcus luteus, and calf thymus.
mesh_terms: D000818:Animals; D002384:Catalysis; D002417:Cattle; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D004247:DNA / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D006609:High Mobility Group Proteins; D008837:Micrococcus / Q000201:enzymology; D008854:Microscopy, Electron; D013950:Thymus Gland / Q000201:enzymology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D004247:DNA; D004264:DNA Topoisomerases, Type I