An enzyme-linked immunoassay (ELISA) for measurement of lactoferrin.
An enzyme-linked immunosorbent assay has been developed for quantitation of lactoferrin (LF) in body fluids. An indirect double-sandwich method was used which allows a sensitivity of 3 ng LF/ml in samples of polymorphonuclear cell lysates and serum. Mean LF content of serum was 0.307 +/- 0.066 micrograms/ml (n = 18). Mean LF content of polymorphonuclear cells was 4.90 +/- 1.48 micrograms/10(6) PMN. Concentrations of LF were similar in serum and in plasma of EDTA anticoagulated blood. Advantages of this method include its rapidity, and radioactivity is not required.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D006801:Humans; D007124:Immunoenzyme Techniques; D007781:Lactoferrin / Q000032:analysis / Q000097:blood / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology*; D009504:Neutrophils / Q000032:analysis / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase / Q000097:blood; D011817:Rabbits; D014168:Transferrin / Q000276:immunology
D007782:Lactoglobulins; D014168:Transferrin; D009195:Peroxidase; D007781:Lactoferrin