title: The effect of lipid peroxidation on transport function in human erythrocytes.
abstract: We have reported that the generation of oxygen free radicals within human red cells resulting from treatment with phenazine methosulfate (PMS) induces several toxic effects in membranes; lipid peroxidation, a binding of methemoglobin and a loss of intracellular K+. The specific K+ carriers are only slightly inhibited (Na+, K+ pump; Na+, K+ cotransport) and the K+ loss is mainly due to an increase in the passive K+ permeability. This could result from a toxic effect on the membrane such as lipid peroxidation or methemoglobin binding. The use of "oxygen free radical scavenger" molecules and pro-oxidative molecules showed that the PMS-dependent increase in passive K+ permeability is consequent to lipid peroxidation rather than to methemoglobin binding.
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D004050:Ditiocarb / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008054:Lipid Peroxides / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood; D008706:Methemoglobin / Q000378:metabolism; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D013481:Superoxides / Q000097:blood*
chemical_list: D008054:Lipid Peroxides; D013481:Superoxides; D008773:Methylphenazonium Methosulfate; D008315:Malondialdehyde; D008706:Methemoglobin; D004050:Ditiocarb