Poliovirus and polio antibody assay in HEp-2 and Vero cell cultures.
HEp-2 cell cultures were about three to 30 times more sensitive for poliovirus titration than Vero cells. Attenuated strains induced a complete cytopathic effect in HEp-2 but not in Vero cells. For polio antibody titration, HEp-2 and Vero cells were equally suitable. A high degree of sensitivity and reproducibility of virus neutralization was achieved in tests utilizing a low virus dose and serum-virus incubation overnight at 36 degrees C. Staining of infected trays with crystal violet obviated reading of viral CPE under the microscope and expedited the evaluation of larger-scale tests.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002478:Cells, Cultured; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology* / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
D000914:Antibodies, Viral