Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
Previous studies with wild-type simian virus 40 DNA have shown that the sequence 5'-GAGGC-3' directs the binding of A protein (T antigen). The functional origin of replication contains four recognition pentanucleotides each of which is separated by a single base pair and arranged a two pairs of direct repetitions that are inverted relative to each other. Analysis of A protein binding to a series of nonviable mutants progressively deleting these contact sites leads to the following conclusions: (i) stable binding of subunits of A protein to three origin pentanucleotides is not sufficient for the initiation of DNA replication, (ii) the stability of DNA binding depends on interactions between bound protein subunits, and (iii) a single pentanucleotide is sufficient to bind and orient a subunit of A protein.
D001483:Base Sequence; D002872:Chromosome Deletion; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D003851:Deoxyribonucleases; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
D004268:DNA-Binding Proteins; D014764:Viral Proteins; D003851:Deoxyribonucleases