Acetyl glyceryl ether phosphorylcholine (platelet-activating factor) mediates heightened metabolic activity in macrophages. Studies on PGE, TXB2 and O2- production, spreading, and the influence of calmodulin-inhibitor W-7.
The phospholipid mediator AGEPC (acetyl glyceryl ether phosphorylcholine) was examined for its effects on guinea pig peritoneal macrophages. At a concentration of 10(-9)-10(-6) M, AGEPC evoked release of prostaglandin E (PGE) and thromboxane B2 (TXB2) from albumin-elicited macrophages. It also triggered generation of O2- by Corynebacterium parvum-induced cells. Moreover, it caused augmented spreading of macrophages. The calmodulin antagonist W-7 attenuated AGEPC-mediated O2- production and cell spreading whereas prostanoid synthesis was enhanced. These novel actions of AGEPC on the major cellular component of the inflammatory process attest to its role as a potent mediator of immunoinflammatory responses.
D000818:Animals; D006168:Guinea Pigs; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011458:Prostaglandins E / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D013929:Thromboxane B2 / Q000378:metabolism*; D013931:Thromboxanes / Q000378:metabolism*; D014665:Vasodilator Agents / Q000494:pharmacology*
D010972:Platelet Activating Factor; D011458:Prostaglandins E; D013449:Sulfonamides; D013931:Thromboxanes; D014665:Vasodilator Agents; D013481:Superoxides; D013929:Thromboxane B2; C017967:W 7; D010100:Oxygen