[Isolation of new transforming viruses from "phenotypically transformed" Sirc cells in a medium with a low concentration of serum or calcium ions].
Possibility of rapid generation of highly transforming viruses from rabbit epithelial corneal cells (Sirc) on the basis of SSAV virus is discussed. The results of the study suggested a relationship between the phenomenon of "phenotypic transformation" with expression of cellular genes and a potential transforming activity. Sirc/SSAV phenotypic transformation was induced by cultivating of cells in a medium with low levels of serum or calcium ions. Subsequent infection of mink lung fibroblasts resulted in generation of 2 new transforming SSAV isolates which induced transformation in cellular lines permissive for SSAV replication. The foci of transformation were of various morphological patterns.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002472:Cell Transformation, Viral; D003470:Culture Media / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007106:Immune Sera / Q000494:pharmacology; D008907:Mink; D009857:Oncogenes; D010641:Phenotype; D011817:Rabbits; D051381:Rats; D012190:Retroviridae / Q000302:isolation & purification*; D012842:Sarcoma Virus, Woolly Monkey / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods
D003470:Culture Media; D007106:Immune Sera; D002118:Calcium
Poluchenie novykh transformiruiushchikh virusov iz kletok Sirc, "fentotipicheski transformirovannykh" v srede s nizkoĭ kontsentratsieĭ syvorotki ili ionov kal'tsiia.