Comparison of some storage and isolation methods to recover bluetongue virus from bovine blood.
Bovine blood containing bluetongue virus was stored as whole blood with EDTA (4 degrees C), as washed cellular components (4 degrees C), and as washed cellular components with 10% DMSO (-70 degrees C). Periodic isolation attempts were made over a period of 330 days in four cell lines and embryonating chicken eggs (intravenous inoculation). Bluetongue virus was successfully isolated in all systems from most samples throughout the test period. There appeared to be more variation amongst days of attempted isolation within systems than between systems.
D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary*; D001819:Bluetongue / Q000382:microbiology*; D001820:Bluetongue virus / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D008297:Male; D012087:Reoviridae / Q000302:isolation & purification*; D012756:Sheep; D014766:Viremia / Q000382:microbiology / Q000662:veterinary*; D014776:Virus Cultivation / Q000379:methods*