[Role of the protein fraction of Bordetella pertussis in inducing experimental allergic encephalomyelitis].
B. pertussis protein fraction obtained by precipitation with trichloroacetic acid (TCA) stimulated the development of clinically and histologically pronounced experimental allergic encephalomyelitis (EAE) in guinea pigs when introduced together with a heterologous cerebral antigen, the two compounds forming an encephalitogenic mixture. The adjuvant activity of the TCA-precipitated fraction depended on its dose. The sera of the animals with EAE induced by the encephalitogenic mixture containing B. pertussis cells or TCA-precipitated fraction showed a cytopathogenic effect in the monolayer culture of newborn rat cerebellum cells. The cytopathogenic effect was more pronounced in the sera obtained at the period of the development of the clinical symptoms of the disease (days 14-18), while the cytotoxic effect of the sera obtained on day 30 after immunization decreased irrespective of the manifestations of EAE, this decrease being in correlation with the dose of the TCA-precipitated fraction in the encephalitogenic mixture.
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000633:toxicity*; D001886:Bordetella pertussis / Q000472:pathogenicity*; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology* / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D007526:Isoelectric Point; D008297:Male; D013997:Time Factors; D014238:Trichloroacetic Acid / Q000494:pharmacology
D001426:Bacterial Proteins; D014238:Trichloroacetic Acid
Rol'belkovoĭ fraktsii kokliushnykh mikrobov v induktsii eksperimental'nogo allergicheskogo entsefalomielita.