Long-term survival and aldosterone secretion pattern of aldosteronoma in culture.
In an attempt to obtain an in vitro experimental model for aldosteronoma, primary culture was initiated with adenomas from 3 patients with primary aldosteronism. The cells grown in culture retained the morphology and functional properties characteristic of aldosteronoma cells well for periods of up to 200 days. The cells formed monolayer cell colonies and showed an epithelioid morphology with small nuclei containing prominent nucleoli. The cells possessed a clear, eosinophilic cytoplasm resembling that of aldosteronoma cells in vivo. The cultured cells continued to secrete large amounts of aldosterone throughout the culture period. The cells responded to angiotensin II and III by increased release of aldosterone into the culture medium. They also responded to Db-cAMP and ACTH by increased secretion of the hormone.
D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000378:metabolism*; D000803:Angiotensin I / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism / Q000473:pathology
D000804:Angiotensin II; D000450:Aldosterone; D003994:Bucladesine; D000324:Adrenocorticotropic Hormone; D000803:Angiotensin I