Proteolysis of vimentin and desmin by the Ca2+-activated proteinase specific for these intermediate filament proteins.
The degradation of vimentin and desmin by the Ca2+-activated proteinase specific for these intermediate filament proteins proceeds in two stages in the form of a limited proteolysis. At first, the reaction is very rapid, with the stepwise and complete removal of a peptide (ca. 9,000 daltons) from the N-terminal of vimentin and desmin. This results in the production of a characteristic "staircase" of degradation products, as seen in two-dimensional polyacrylamide gel electrophoresis. The second stage of proteolysis is characterized by the accumulation of peptides which are resistant to further proteolysis; this is due not to product inhibition but to the fact that these peptides are not substrates for the proteinase and therefore do not protect the latter from inactivation (autodigestion). In vitro phosphorylation of the substrates does not affect proteinase activity, probably because the phosphorylation site is located towards the C-terminal of the molecules. The specific and limited proteolysis of vimentin and desmin results in the deletion of the nucleic acid binding and filament assembly site of these proteins, indicating that the Ca2+-activated proteinase plays a role in regulating the function(s) of these intermediate filament proteins, rather than their simple turnover during the cell cycle.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002154:Calpain; D002286:Carcinoma, Ehrlich Tumor; D003893:Desmin; D010450:Endopeptidases / Q000378:metabolism*; D006868:Hydrolysis; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009696:Nucleic Acids / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D013379:Substrate Specificity; D013552:Swine; D014746:Vimentin
D003893:Desmin; D007381:Intermediate Filament Proteins; D009696:Nucleic Acids; D010446:Peptide Fragments; D010750:Phosphoproteins; D014746:Vimentin; D010450:Endopeptidases; D002154:Calpain