A comparison between the metabolism of delta 1-tetrahydrocannabinol by perfused lung and liver of rat and guinea-pig.
The metabolism of delta 1-tetrahydrocannabinol (delta 1-THC) by lung and liver of rat and guinea-pig have been studied by isolated perfused-organ techniques. Metabolites were identified by t.l.c. and mass fragmentography. Biotransformation in lungs showed a different quantitative pattern to the livers in both species. 4"-Hydroxy-delta 1-THC was a major metabolite in both rat and guinea-pig lung. 7-Hydroxy-delta 1-THC was another major metabolite in guinea-pig lung, but a minor one in rat lung. 6 alpha-Hydroxy-delta 1-THC and 6,7-dihydroxy-delta 1-THC were formed in small quantities in both species. Small amounts of 6 beta-hydroxy-delta 1-THC were detected in guinea-pig lung in addition to the metabolites mentioned. Livers of rat and guinea-pig formed 7-hydroxy-delta 1-THC as the most abundant metabolite together with minor amounts of 6 alpha-hydroxy- and 6,7-dihydroxy-delta 1-THC. 6 beta-Hydroxy-delta 1-THC was a minor liver metabolite in guinea-pig, and was found in rat only in trace amounts, together with delta 1-THC-6-one. No side-chain hydroxylated metabolites were detected in rat liver, while guinea-pig liver formed trace amounts of 3"-hydroxy- and 4"-hydroxy-delta 1-THC.
D000818:Animals; D013759:Dronabinol / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D013759:Dronabinol