Low infectivity of vesicular stomatitis virus (VSV) particles released from interferon-treated cells is related to glycoprotein deficiency.
We have investigated the mechanism for the low infectivity of vesicular stomatitis virus (VSV) released from interferon (IFN) -treated cells. With 10-30 units/ml of IFN there was an approximately 5-30 fold reduction in the production of virus particles, as measured by VSV proteins; however, the infectivity of the VSV released from IFN-treated mouse LB, JLS-V9R, or human GM2504 was drastically reduced (2 to 4 logs). The low infectivity of VSV was directly related to a deficiency in virion glycoprotein (G). IFN treatment did not change the specific infectivity of the VSV particles released by HeLa cells; their G protein was also not reduced. A further effect of IFN to reduce the amount of virion M protein appeared to be secondary and was probably not related to the reduced infectivity of VSV.
D000818:Animals; D002460:Cell Line; D006023:Glycoproteins / Q000235:genetics*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007739:L Cells / Q000382:microbiology; D008297:Male; D051379:Mice; D012867:Skin; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D006023:Glycoproteins; D007370:Interferon Type I; D014764:Viral Proteins