title: Cryo-electron microscopy of viruses.
abstract: Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.
mesh_terms: D000260:Adenoviruses, Human / Q000648:ultrastructure; D010582:Bacteriophage lambda / Q000648:ultrastructure; D004926:Escherichia coli / Q000648:ultrastructure; D005615:Freezing; D008854:Microscopy, Electron / Q000379:methods; D012672:Semliki forest virus / Q000648:ultrastructure; D013604:T-Phages / Q000648:ultrastructure; D014721:Vesicular stomatitis Indiana virus / Q000648:ultrastructure; D014780:Viruses / Q000648:ultrastructure*