title: Intragenic suppressor mutations that restore export of maltose binding protein with a truncated signal peptide.
abstract: A deletion mutation, malE delta 12-18, removes seven residues from the hydrophobic core of the maltose binding protein (MBP) signal peptide and thus prevents secretion of this protein to the periplasm of E. coli. Intragenic suppressor mutations of malE delta 12-18 have been obtained, some highly efficient in their ability to restore proper MBP export. Twelve independently isolated suppressors represent six unique mutational events. Five result in alterations within the MBP signal peptide; one changes the amino acid at residue 19 of the mature MBP. Analysis of these suppressors indicates that the length of the hydrophobic core is a major determinant of signal peptide function. The experiments further suggest that the hydrophobic core region serves primarily a structural role in mediating protein secretion, and that other sequences outside of this region may be responsible for providing the initial recognition of the MBP nascent chain as a secreted protein.
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005796:Genes; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D010455:Peptides / Q000235:genetics*; D033902:Periplasmic Binding Proteins; D010641:Phenotype; D010957:Plasmids; D021382:Protein Sorting Signals; D013489:Suppression, Genetic; D013698:Templates, Genetic
chemical_list: D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D010455:Peptides; D033902:Periplasmic Binding Proteins; D021382:Protein Sorting Signals; C461599:maltose transport system, E coli; D004262:DNA Restriction Enzymes