title: Terminal and internal inverted repetitions in African swine fever virus DNA.
abstract: An electron microscopic analysis of the heteroduplexes formed by reannealing denatured terminal restriction fragments of African swine fever (ASF) virus DNA showed Y-shaped molecules with a 2.1-kilobase-pair-long double-stranded tail and two single-stranded arms. This indicated that ASF virus DNA has terminal inverted repetitions with a length of 2.1 kbp. In addition, under less restrictive hybridization conditions, most of the heteroduplexes showed a 0.13 kbp-long internal double-stranded region, separated from the long terminal repeat by a single-stranded asymmetric loop. These internal inverted repetitions did not match well, since the heteroduplexes melted under conditions where those of the terminal repetitions were stable. In the terminal fragments EcoRI-K' and D', the distance between the terminal and the internal inverted repetitions was 2.4 and 0.4 kbp, respectively.
mesh_terms: D000358:African Swine Fever Virus / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D007497:Iridoviridae / Q000235:genetics*; D007668:Kidney; D008854:Microscopy, Electron; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes