title: Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein.
abstract: The nucleotide sequence of the gene that codes for the major inner capsid protein of the simian rotavirus SA11 has been determined. A DNA copy of mRNA from gene 6 was cloned in the E. coli plasmid pBR322. The full-length gene is 1357 nucleotides long with a 5'-noncoding region of 23 nucleotides and a 3'-noncoding region of 140 nucleotides. The gene contains a single, long, open reading-frame of 1194 nucleotides capable of coding for a protein of 397 amino acids with a molecular weight of 44,816. The predicted protein product is relatively proline-rich with a net charge at neutral pH of -3.5. One stretch of 53 amino acids (encoded by nucleotides 327-485) is basic.
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008970:Molecular Weight; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification; D015678:Viral Structural Proteins
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins; D015678:Viral Structural Proteins