Enhancement by sulfatide of Na+-independent [3H]GABA binding in mouse brain.
The effect of exogenous sulfatide on [3H]gamma-aminobutyric acid binding to postsynaptic receptors was investigated. Sulfatide (0.1 mM) increased the high affinity binding sites by 67% when it was incubated with membrane tissue at 37 degrees C for 20 min prior to [3H]gamma-aminobutyric acid binding assay. Preincubation of 0.05% Triton X-100 treated tissue with 0.1 mM sulfatide at 37 degrees C for 20 min augmented the high and low affinity binding sites by 16.4 and 16.2%, respectively. The results of the present study indicate that sulfatide may play a significant role in contributing to the integrity of gamma-aminobutyric acid receptors.
D000818:Animals; D001923:Brain Chemistry; D005615:Freezing; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017830:Octoxynol; D011092:Polyethylene Glycols; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D011963:Receptors, GABA-A; D012964:Sodium / Q000378:metabolism*; D013433:Sulfoglycosphingolipids / Q000494:pharmacology* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D013433:Sulfoglycosphingolipids; D011092:Polyethylene Glycols; D005680:gamma-Aminobutyric Acid; D017830:Octoxynol; D012964:Sodium