[Routine detection of the THC metabolite 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid in forensic practice].
A method for the detection of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid, one of the predominant metabolites of delta 9-tetrahydrocannabinol in human urine, is presented. As screening method a homogeneous enzyme immunoassay is used (Emit dau). Positive results are confirmed by GLC/MS as alternative method. After glucuronide hydrolysis the metabolite is isolated using a C18 reversed phase column and methylated in the 1-hydroxy and 9-carboxy position. The methylated metabolite is detected by gas chromatography/mass fragmentography. This fairly rapid method seems to be useful in forensic routine analysis.
D013759:Dronabinol / Q000031:analogs & derivatives* / Q000652:urine; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D002189:Marijuana Abuse / Q000652:urine*
C026502:delta(9)-tetrahydrocannabinol-11-oic acid glucuronide; D013759:Dronabinol
Routine-Nachweis des THC-Metaboliten 11-Nor-delta 9-tetrahydrocannabinol-9-carbonsäure in der forensischen Praxis.