title: Detection of a molecular complex between ras proteins and transferrin receptor.
abstract: Immunoprecipitation of extracts of human carcinoma cell lines with three different monoclonal antibodies generated against ras proteins revealed the coprecipitation of a 90,000 dalton protein. The coprecipitated protein was identified as the transferrin receptor by comigration in both reducing and nonreducing SDS-polyacrylamide gels, by absorption with a monoclonal antibody directed against transferrin receptor, and by analysis of partial proteolysis products. Coprecipitation of the transferrin receptor with three monoclonal antibodies with differing specificities to ras proteins, as well as the inability to coprecipitate the transferrin receptor from cell extracts from which ras proteins were depleted by preabsorption, indicates that ras proteins and the transferrin receptor form a molecular complex. This complex is disrupted by addition of transferrin to cell extracts. These findings suggest that ras proteins function in regulation of cell growth via interaction with the cell surface receptor for transferrin.
mesh_terms: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002273:Carcinogens; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics*; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis; D011956:Receptors, Cell Surface / Q000302:isolation & purification; D011990:Receptors, Transferrin; D014168:Transferrin / Q000302:isolation & purification; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002273:Carcinogens; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin