Establishment of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive nasopharyngeal carcinoma hybrid cell line (NPC-KT).
Primary nasopharyngeal carcinoma (NPC) cells were fused to hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-defective cells derived from adenoid tissues using Sendai virus. Some of the fused cells developed into epithelial-like hybrid cells in a selective HAT medium. The hybrid cells (NPC-KT) were Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive cells. There have been no reports on the establishment of EBNA-positive epithelial cell lines derived from NPC. Thus, the epithelial-like hybrid cells might serve as an in vitro model for studying the biologic activity of NPC-associated EBV.
D000368:Aged; D000956:Antigens, Viral / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002459:Cell Fusion; D002460:Cell Line; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D009303:Nasopharyngeal Neoplasms / Q000276:immunology*
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007041:Hypoxanthine Phosphoribosyltransferase