Schwann cell galactocerebroside induced by derivatives of adenosine 3',5'-monophosphate.
Indirect immunofluorescence was used to show the presence of galactocerebroside (galC), a lipid found in myelin, on the surface of about half of the Schwann cells isolated from neonatal rat sciatic nerves and cultured for 1 day without neurons. By day 4 in vitro, the Schwann cells had all lost their surface galC. Three days after beginning treatment with 10(-3) molar 8-bromo-adenosine 3',5'-monophosphate (8-bromo cyclic AMP) or N6,O2'-dibutyryl adenosine 3',5'-monophosphate (dibutyryl cyclic AMP), galC reappeared on the Schwann cells, and 2 days later 48 percent of the cultured Schwann cells showed surface galC. Tritium from tritiated D-galactose was incorporated into galC by the 8-bromo cyclic AMP-and dibutyryl cyclic AMP-treated cultures at a rate 15 times the control rate.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002478:Cells, Cultured; D002554:Cerebrosides / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005699:Galactosylceramides / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects* / Q000378:metabolism; D012584:Sciatic Nerve / Q000378:metabolism
D002087:Butyrates; D002554:Cerebrosides; D005699:Galactosylceramides; D020148:Butyric Acid; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000249:Adenosine Monophosphate; D003994:Bucladesine