Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.
Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.
D004247:DNA / Q000032:analysis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens; D006720:Homozygote; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008285:Major Histocompatibility Complex; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011110:Polymorphism, Genetic
D006684:HLA-DR Antigens; D004247:DNA; D004262:DNA Restriction Enzymes