Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.
Cloned DNA copies of the double-stranded RNA genomic segments of simian 11 rotavirus have been used to determine the coding assignment for VP7, the type-specific antigen of this virus. Translation of hybrid-selected mRNAs in an in vitro system supplemented with canine pancreatic microsomes permitted VP7 to be assigned to segment 9 and the two nonstructural viral proteins NCVP4 and NCVP3, to segments 7 and 8, respectively. Hybridization of cloned DNA probes for segments 7-9 with the corresponding segments of human rotavirus Wa confirmed these assignments. The complete nucleotide sequence of gene 9 has been determined. The deduced amino acid sequence reveals VP7 to be 326 amino acids in length with two NH2-terminal hydrophobic regions and a single glycosylation site at residues 69-71.
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics; D014176:Protein Biosynthesis; D012401:Rotavirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins