[Characteristics of rat kidney cell lines transformed by the DNA of human adenovirus type 6].
The data on the transforming activity of intact DNA of human adenovirus type 6 and that fragmented by restrictases Bam H I (31.3% of the genome), Bgl 2 (9.3% of the genome), and Hind 3 (7.6% of the genome) in a primary culture of rat kidney cells are presented. The activity of the specimens varied within 1.1-2.1 foci per 1 microgram of DNA. From the transformation foci 5 cell lines were derived and their properties (T-antigen production, integration of adenovirus genome, phenotypic features) were studied.
D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000494:pharmacology*; D005814:Genes, Viral; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000276:immunology / Q000382:microbiology; D051381:Rats; D014162:Transfection
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Kharakteristika liniĭ kletok pochek krys, transformirovannykh DNA adenovirusa cheloveka tipa 6.