Oxidation state dependence of proton exchange near the iron-sulfur centers in ferredoxins and high-potential iron-sulfur proteins.
For both the [2Fe-2S] and the [4Fe-4S] ferredoxins, dialysis against 2H2O prior to single electron reduction leads to the appearance of a deuterium modulation pattern in the electron spin echo decay envelope indicative of deuteron-proton exchange very near the paramagnetic center. In contrast, if the ferredoxin is exposed to 2H2O after its reduction in H2O, far less deuterium exchange near the metal center takes place. Thus, proton exchange with solvent is in part dependent on the redox state of the protein. For high potential iron-sulfur proteins, this type of proton-deuteron exchange near the metal center does not occur unless the protein is partially unfolded in dimethylsulfoxide in 2H2O.
D000325:Adrenodoxin / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003903:Deuterium; D017666:Deuterium Oxide; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism*; D008872:Microwaves; D010084:Oxidation-Reduction; D014867:Water
D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D014867:Water; D000325:Adrenodoxin; D003903:Deuterium; D017666:Deuterium Oxide