Proline hydroxylation by cell free extract of a streptomycete.
Free L-proline was hydroxylated to free L-hydroxyproline by cell free extract of Streptomyces griseoviridus P8648. The hydroxylation reaction required ferrous ion, 2-ketoglutarate and ascorbate. Zinc ion, ethylenediaminetetraacetic acid and alpha,alpha'-dipyridyl inhibited the reaction. Optimum temperature and pH were 25.0 degrees C and 7.5, respectively.
D006863:Hydrogen-Ion Concentration; D006909:Hydroxyproline / Q000096:biosynthesis; D007700:Kinetics; D011393:Procollagen-Proline Dioxygenase / Q000302:isolation & purification*; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature
D011393:Procollagen-Proline Dioxygenase; D006909:Hydroxyproline