title: Effects of various secretagogues on gonadotropin release by rat anterior pituitary cell cultures.
abstract: Rat anterior pituitary cell cultures were utilized to compare LH and FSH release induced by LHRH, cAMP derivatives, cGMP derivatives, the calcium ionophore A23187, and elevated K+. Of the cyclic nucleotide derivatives tested, only 8-Br-cAMP released significant quantities of both LH and FSH. A23187 and elevated K+ released significant quantities of LH and FSH but supramaximal concentrations of these stimuli liberated only approximately 50% of the gonadotropins released by LHRH. Thus, none of the secretagogues tested was as efficacious as LHRH in releasing either LH or FSH.
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats
chemical_list: D015124:8-Bromo Cyclic Adenosine Monophosphate; D007987:Gonadotropin-Releasing Hormone; D000001:Calcimycin; D003994:Bucladesine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D011188:Potassium