94,000- and 100,000-molecular-weight simian virus 40 T-antigens are associated with the nuclear matrix in transformed and revertant mouse cells.
A small fraction of the 94,000-molecular-weight multifunctional large T-antigen of simian virus 40 was associated with the nuclear protein matrix derived from simian virus 40-transformed mouse cells. The interaction between this fraction of T-antigen and the matrix was largely or entirely independent of nuclear DNA. Similar amounts of T-antigen were retained by the nuclei of transformed and revertant cell lines. A 100,000-molecular-weight variant of T-antigen, which has been found to correlate specifically with anchorage-independent growth, was present in the nuclear protein matrix of a transformed cell line. A T-antigen-containing revertant selected for the reacquisition of a high serum requirement and an anchorage requirement for growth retained T-antigen in association with its matrix.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D000957:Antigens, Viral, Tumor; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D002472:Cell Transformation, Viral; D051379:Mice; D008970:Molecular Weight; D013539:Simian virus 40 / Q000276:immunology* / Q000502:physiology
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor