Subcloning of the cloacin DF13/aerobactin receptor protein and identification of a pColV-K30-determined polypeptide involved in ferric-aerobactin uptake.
A plasmid containing a pColV-K30 fragment that encoded only for the cloacin DF13/aerobactin receptor protein was constructed. Escherichia coli cells harboring this plasmid were sensitive to cloacin DF13 but were unable to take up ferric-aerobactin. Another pColV-K30-determined polypeptide (molecular weight, 50,000), localized in the membrane fraction, was essential for the uptake of ferric-aerobactin.
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D001430:Bacteriocins; D001692:Biological Transport; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D006877:Hydroxamic Acids / Q000378:metabolism*; D007700:Kinetics; D010957:Plasmids; D011971:Receptors, Immunologic / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; D006877:Hydroxamic Acids; D011971:Receptors, Immunologic; C034506:aerobactin receptor; C027355:cloacin DF13 protein, E coli; C031819:aerobactin; D004262:DNA Restriction Enzymes