Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol.
The ability of purified Rous sarcoma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol was phosphorylated to form both mono- and diphosphorylated derivatives. 1,2-Diacylglycerol was phosphorylated to form phosphatidic acid. These activities showed the same thermolability and the same sensitivity to inhibitors as shown by the casein kinase activity of pp60v-src. In addition, when serum-starved chicken embryo fibroblasts transformed by a virus mutant temperature-sensitive for transformation were shifted from the nonpermissive to permissive temperature, an increase of 50-100% in the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidic acid was observed, as compared to uninfected cells.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002417:Cattle; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D004075:Diglycerides / Q000378:metabolism*; D004789:Enzyme Activation; D005347:Fibroblasts / Q000201:enzymology; D005796:Genes; D005814:Genes, Viral; D005989:Glycerides / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D004075:Diglycerides; D005989:Glycerides; D010716:Phosphatidylinositols; D014764:Viral Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)