Biochemical cascade mediating the estradiol action.
The data suggest that estradiol enhances the formation of histamine in rat uterus by induction of histidine decarboxylase; histamine activates adenylate cyclase providing accumulation of cyclic 3',5'-AMP, which, probably, induces glycolytic enzymes via phosphorylation of chromatin proteins, and mediates other estradiol effects. The chain of successively acting enzymes and mediators constitutes, obviously, a cascade amplifying the estradiol action. Since histamine is known to act as an intercellular mediator, attempts were made to find out the distribution of estradiol, histamine, and cyclic 3',5'-AMP among uterine cells. Autoradiography has shown that 3H-estradiol is bound by the nuclei of myometrium cells, 3H-histamine was found in the cytoplasm of these cells, 3H-cyclic 3',5'-AMP is selectively bound by the cells of capillary endothelium of the uterus. The estradiol mediators seem to spread their effect on different types of cells which form together a kind of a multicellular functional system.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D006640:Histidine Decarboxylase / Q000096:biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D004958:Estradiol; D006632:Histamine; D000242:Cyclic AMP; D011494:Protein Kinases; D006640:Histidine Decarboxylase; D000262:Adenylyl Cyclases