Ferric chloride flocculation for nonflocculating beef extract preparations.
The addition of 2.5 mM ferric chloride to 0.5% beef extract solution at pH 3.5 was found to be highly efficient in the recovery of seeded poliovirus type 1 (Sabin) or indigenous viruses from environmental samples. This method was extremely useful to reconcentrate viruses from beef extract solutions that did not flocculate at pH 3.5.
D002712:Chlorides; D005290:Ferric Compounds; D005427:Flocculation; D007501:Iron; D008460:Meat; D017955:Poliovirus / Q000302:isolation & purification*; D012722:Sewage / Q000032:analysis; D014020:Tissue Extracts; D014776:Virus Cultivation; D014871:Water Microbiology
D002712:Chlorides; D005290:Ferric Compounds; D012722:Sewage; D014020:Tissue Extracts; D007501:Iron; C024555:ferric chloride