Segregation and mapping analysis of polymorphic HLA class I restriction fragments: detection of a novel fragment.
An HLA-B7 complementary DNA clone was used as a hybridization probe to analyze the segregation pattern of polymorphic class I restriction fragments in several families whose HLA types had been determined by serological techniques. In one family in which a crossover in the HLA region had occurred, a specific genomic fragment was mapped with respect to the crossover. In another family, a novel genomic fragment present in one child and absent in all other family members was observed. With the exception of this novel fragment, all polymorphic class I fragments observed in this study segregated with a serologically defined parental haplotype, a result consistent with HLA linkage.
D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005260:Female; D005802:Genes, MHC Class II; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic
D006680:HLA Antigens; D004262:DNA Restriction Enzymes