New method for the purification of 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase (E.C. 2.7.6.3) from Escherichia coli. Application of hydrophobic interaction chromatography and flat-bed isoelectric focusing.
D002845:Chromatography; D017854:Diphosphotransferases; D004926:Escherichia coli / Q000201:enzymology*; D007525:Isoelectric Focusing; D010770:Phosphotransferases / Q000302:isolation & purification*
D010770:Phosphotransferases; D017854:Diphosphotransferases; C019684:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase