title: Effect of acclimation temperature on the concentration of the mitochondrial 'uncoupling' protein measured by radioimmunoassay in mouse brown adipose tissue.
abstract: The effect of acclimation temperature on the concentration of the mitochondrial 'uncoupling' protein (Mr 32000) from brown adipose tissue of mice has been investigated. The uncoupling protein was measured by a specific radioimmunoassay. Between 33 degrees C (thermoneutrality) and -2 degrees C there was a progressive increase with decreasing environmental temperature in the amount of uncoupling protein. For mice at -2 degrees C the mitochondrial concentration of the protein was 9-times higher than at 33 degrees C, while the total amount of the protein in interscapular brown adipose tissue was estimated to be nearly 80-times greater at -2 degrees C compared to 33 degrees C.
mesh_terms: D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D001835:Body Weight; D002352:Carrier Proteins; D003576:Electron Transport Complex IV / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins; D011863:Radioimmunoassay; D013696:Temperature; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D006153:Guanosine Diphosphate; D003576:Electron Transport Complex IV