title: Autoradiographic analysis of proestrous changes in the binding of 125I-labeled prolactin to the hamster ovary.
abstract: Autoradiographic histochemistry was employed to examine changes in the binding of 125I-labeled prolactin (Prl) to ovaries from proestrous hamsters before (at 1200 h), during (at 1600 h), and after (at 2000 h) the preovulatory gonadotropin surge. In untreated control hamsters, there was a marked and progressive loss of Prl binding, first in the interstitial cells and follicular thecae by 1600 h, and then in the granulosa cells of the preovulatory follicles by 2000 h. When proestrous hamsters were treated with ergocryptine to significantly lower serum Prl, or injected with exogenous Prl, Prl binding to their ovaries did not differ from controls, suggesting that decreased Prl binding was due to neither increased occupancy of binding sites by endogenous Prl nor down regulation of Prl receptors by Prl itself. Conversely, when proestrous hamsters were treated with phenobarbital to block the luteinizing hormone (LH)/follicle-stimulating hormone (FSH) surge, the loss of Prl binding sites in the ovaries was prevented, suggesting that the LH/FSH surge might initiate a down regulation of Prl receptors in the ovary. Such a down regulation of Prl receptors may serve as a mechanism by which the ability of Prl to affect periovulatory events in the ovary might be regulated.
mesh_terms: D000818:Animals; D001345:Autoradiography; D006224:Cricetinae; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008647:Mesocricetus; D010053:Ovary / Q000378:metabolism*; D011247:Pregnancy; D011359:Proestrus; D011388:Prolactin / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D011981:Receptors, Prolactin
chemical_list: D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone