Tryptophan hydroxylase and 5-HTP-decarboxylase activity in cockroach brain and the effects of p-chlorophenylalanine and 3-hydroxybenzylhydrazine (NSD-1015).
The activities of two enzymes of the serotonin biosynthetic pathway, namely tryptophan hydroxylase (TPH) and 5-hydroxytryptophan-decarboxylase (5-HTP-D), were determined in the cockroach, Periplaneta americana, brain using radiometric techniques, significant levels of both enzymes were found. p-Chlorophenylalanine (PCPA) inhibited brain TPH activity strongly both in vitro and in vivo. In vitro, 10 mM PCPA reduced the TPH activity by 76% and in vivo either 15 micrograms/g or 30 micrograms/g of PCPA inhibited brain TPH activity by 80-85%. The activity of 5-HTP-D enzyme was inhibited significantly in vivo by 3-hydroxybenzylhydrazine, commonly known as NSD-1015. A recovery in the activity of 5-HTP-D enzyme was observed 12 h after 50 micrograms/g of NSD-1015 but 100 micrograms/g caused an inhibition lasting for the 24 h period studied.
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D003058:Cockroaches / Q000378:metabolism*; D010134:Fenclonine / Q000494:pharmacology*; D005724:Ganglia / Q000201:enzymology* / Q000378:metabolism; D006834:Hydrazines / Q000494:pharmacology*; D010526:Periplaneta / Q000378:metabolism*; D012701:Serotonin / Q000096:biosynthesis; D009435:Synaptic Transmission; D014365:Tryptophan Hydroxylase / Q000378:metabolism*
D006834:Hydrazines; D012701:Serotonin; C004297:3-hydroxybenzylhydrazine; D014365:Tryptophan Hydroxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D010134:Fenclonine