title: Monocyte superoxide anion production in rheumatoid arthritis: preliminary evidence for enhanced rates of superoxide anion production by monocytes from patients receiving penicillamine, sodium aurothiomalate and corticosteroids.
abstract: In-vitro studies of superoxide (O-2) anion production by blood monocytes after stimulation with either serum treated zymosan (STZ), IgG treated zymosan (IgGTZ), or fluoride ion (F-) were performed on cells from normal controls (n = 22) and patients with classical or definite rheumatoid arthritis (RA) (n = 35). Twenty-two of the patients were on nonsteroidal anti-inflammatory drugs (NSAID) alone and 13 were on either sodium aurothiomalate, penicillamine, corticosteroids, or a combination. Monocytes from RA patients on 'second-line therapy' showed significantly increased rates of O-2 release in response to STZ compared with normal controls, but no increase was seen in monocytes from patients on NSAID alone. With IgGTZ as the stimulus, rates of O-2 release were increased in monocytes from patients on NSAID alone compared with normal controls (p less than 0.02), but were increased to a greater extent in monocytes from patients on second-line therapy (p less than 0.01). There were no differences in basal unstimulated O-2 production and no differences after stimulation with F-. The enhanced release of O-2 by monocytes from patients on second-line therapy could not be attributed to increased disease activity and may be an effect of therapy.
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000838:Anions / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D006052:Gold Sodium Thiomalate / Q000627:therapeutic use; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D010396:Penicillamine / Q000627:therapeutic use; D013481:Superoxides / Q000378:metabolism*
chemical_list: D000305:Adrenal Cortex Hormones; D000838:Anions; D000893:Anti-Inflammatory Agents; D013481:Superoxides; D006052:Gold Sodium Thiomalate; D010396:Penicillamine