Benzo(a)pyrene and aniline increase sister chromatid exchanges in cultured rat liver fibroblasts without addition of activating enzymes.
The RL4 rat liver epithelial cell line possesses enzymes capable of bioactivating xenobiotics. This cell line was used without the addition of exogenous activating enzymes to study the genotoxicity of benzo(a)pyrene B(a)P and aniline as measured by the sister chromatid exchange technique. Both increased sister-chromatid exchange (SCE) formation in RL4 cells although B(a)P was more effective than aniline. Pyrene, a structural analogue of B(a)P, demonstrated no genotoxicity. These results indicate that the RL4 cell line containing endogenous bioactivation ability may be useful for genotoxicity studies.
D000814:Aniline Compounds / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism / Q000633:toxicity*; D001711:Biotransformation; D002460:Cell Line; D003434:Crossing Over, Genetic / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000814:Aniline Compounds; D001580:Benzopyrenes; D001564:Benzo(a)pyrene; C023650:aniline