Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.
We have used P-element-mediated transformation to introduce a cloned Drosophila alcohol dehydrogenase (Adh) gene into the germ line of ADH null flies. Six independent transformants expressing ADH were identified by their acquired resistance to ethanol. Each transformant carries a single copy of the cloned Adh gene in a different chromosomal location. Four of the six transformant lines exhibit normal Adh expression by the following criteria: quantitative levels of ADH enzyme activity in larvae and adults; qualitative tissue specificity; the size of stable Adh mRNA; and the characteristic developmental switch in utilization of two different Adh promoters. The remaining two transformants express ADH enzyme activity with the correct tissue specificity, but at a lower level than wild type. These results demonstrate that an 11.8 kb chromosomal fragment containing the Adh gene includes the cis-acting sequences necessary for its correct developmental expression, and that a variety of chromosomal sites permit proper Adh gene function.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000502:physiology*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D008297:Male; D009876:Operon; D012333:RNA, Messenger / Q000032:analysis; D014018:Tissue Distribution; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase