The use of discontinuous Percoll gradients to separate populations of cells from human bone marrow and peripheral blood.
Cells from human bone marrow samples were separated on discontinuous Percoll gradients and the effects of variations in osmolality were studied. Yield of committed progenitor cells as determined by CFU-c and BFU-E assays was improved when a gradient of discontinuous osmolality (360-320 mosM) was compared with gradients of uniform osmolality (320 mosM and 290 mosM). The CFU-c and BFU-E containing fraction recovered from this gradient also contained fewer T cells. When used for separation of peripheral blood samples, the same gradient yielded populations of mononuclear cells, neutrophils and platelets of more than 90% purity.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001773:Blood Cells / Q000166:cytology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged; D011205:Povidone; D012822:Silicon Dioxide; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone