The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses.
The use of T1 RNase fingerprinting of terminally labelled genomic double-stranded RNA species from various rotavirus isolates, to analyse the near terminal G-residue positions, has revealed an RNA species-specific fingerprint pattern covering approximately 40 nucleotides at the termini. These RNA species-specific terminal fingerprint patterns were found to be conserved in both rotavirus RNAs isolated from various animal species, and in isolates from a single animal species where gross divergence of internal RNA sequence for a particular RNA species was evident. This conservation of near terminal G-residue positions suggests that, internal to the short regions of absolute terminal sequence conservation that we have previously shown to be present on all rotavirus RNA species, there is a region of conserved sequence which is specific for a particular RNA species.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005814:Genes, Viral; D006801:Humans; D012330:RNA, Double-Stranded / Q000032:analysis / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000032:analysis*; D013045:Species Specificity
D012330:RNA, Double-Stranded; D012367:RNA, Viral