title: Replication of human rotavirus in cell culture.
abstract: Nine strains of human rotavirus were adapted to growth in CV-1 and/or MA-104 cells following pretreatment of virus with trypsin, incorporation of trypsin into culture medium, and use of roller cultures. Immunofluorescence was the most reliable method for the detection of virus replication, although characteristic cytopathic effects were produced sporadically by most isolates. Virus could be readily detected in supernatant fluids of cell cultures and in cell sections by electron microscopy.
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D005243:Feces / Q000382:microbiology; D006801:Humans; D008253:Macaca mulatta; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000254:growth & development* / Q000302:isolation & purification / Q000648:ultrastructure; D013696:Temperature; D014357:Trypsin
chemical_list: D012367:RNA, Viral; D014357:Trypsin