Effects of vitamin D3 on the uptake and release of calcium by isolated intestinal mitochondria.
Administration of vitamin D3 or 1,25 (OH)2D3 to rachitic chicks produces a decrease of 45Ca uptake by mitochondria from intestinal mucosa. This effect of vitamin D3 shows tissue specificity, since it was not observed in liver mitochondria from the same animals. The Km values were similar (about 10 microM) for intestinal mitochondria from rachitic and treated animals. The Ca2+ efflux in previously loaded mitochondria was not changed by treatment. The Ca content of recently isolated mitochondria was strikingly lower after vitamin D3 administration. It is concluded that vitamin D3 may participate in the mechanism which regulates the intramitochondrial Ca concentration.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002645:Chickens; D002762:Cholecalciferol / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism
D002762:Cholecalciferol; D002118:Calcium