[Demonstration of specific receptors for gastric inhibitory peptide (GIP)].
Receptors for the Gastric Inhibitory Polypeptide (GIP) were characterized in particles enriched in plasma membranes obtained from a transplantable hamster insulinoma. Native GIP, at concentrations between 10(-10) and 10(-6) M, inhibited competitively the binding of 125I-GIP to membranes. The binding was highly specific since, among the peptides tested, only the porcine peptide having N-terminal histidine and C-terminal isoleucine amide (PHI), at high concentration (10(-6) M), inhibited by 17% the binding of 125I-GIP. The existence of these receptors suggested that GIP could act directly on beta-cells.
D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D005749:Gastric Inhibitory Polypeptide / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D007340:Insulinoma / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis*; D011964:Receptors, Gastrointestinal Hormone
D005768:Gastrointestinal Hormones; D011956:Receptors, Cell Surface; D011964:Receptors, Gastrointestinal Hormone; D005749:Gastric Inhibitory Polypeptide; C041086:gastric inhibitory polypeptide receptor
Mise en évidence de récepteurs spécifiques du peptide inhibiteur gastrique (GIP).