Presynaptic distribution of the cholinergic-specific antigen Chol-1 and 5'-nucleotidase in rat brain, as determined by complement-mediated release of neurotransmitters.
Nerve terminals prepared from rat cortex and hippocampus were loaded with seven radioactive putative neurotransmitters (serotonin, noradrenaline, dopamine, gamma-aminobutyric acid, aspartate, glutamate, and taurine). The release of these transmitters, choline acetyltransferase, 3,4-dihydroxyphenylalanine decarboxylase, enolase, and lactate dehydrogenase was monitored during complement-mediated lysis. Three antisera were used: anti-5'-nucleotidase, anti-Chol-1, and anti-rat cerebrum. Anti-5'-nucleotidase serum did not cause the release of any labelled transmitter or of any of the enzymes studied. Anti-Chol-1 serum released choline acetyltransferase and small amounts of enolase and lactate dehydrogenase. Anti-rat cerebrum caused the release of all seven transmitters, choline acetyltransferase, and small amounts of the other three enzymes. It was concluded that 5'-nucleotidase was not present on any of the terminals studied, and that Chol-1 is only present on cholinergic terminals.
D015720:5'-Nucleotidase; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004296:Dopa Decarboxylase / Q000378:metabolism; D005732:Gangliosides; D006651:Histocytochemistry; D007158:Immunologic Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D014018:Tissue Distribution
D000954:Antigens, Surface; D005732:Gangliosides; C038777:chol-1 antigen; D007770:L-Lactate Dehydrogenase; D002795:Choline O-Acetyltransferase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D004296:Dopa Decarboxylase; D010751:Phosphopyruvate Hydratase