Effect of polyethyleneglycols (PEG 600 and PEG 6000) and bovine serum albumin on myeloperoxidase-mediated release of [3H]-serotonin from rat peritoneal mast cells in vitro.
Rat peritoneal mast cells in vitro released [3H]-serotonin in the presence of halide (I-), myeloperoxidase (MPO), and a H2O2-generating system. The degranulation was partly inhibited with low concentrations of bovine serum albumin (BSA; 0.1-1% w/v), or polyethyleneglycols (PEG 600 or PEG 6000, 0.1% w/v). Furthermore, PEG 600 and PEG 6000 enhanced the stabilization achieved with 0.1% BSA. The results may provide a new aspect on antigenicity of PEG-modified allergens, and on mast-cell response at an inflammatory site.
D000818:Animals; D004347:Drug Interactions; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D009195:Peroxidase / Q000494:pharmacology*; D010544:Peroxidases / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
D012710:Serum Albumin, Bovine; D012701:Serotonin; D011092:Polyethylene Glycols; D010544:Peroxidases; D009195:Peroxidase