Immunological studies of beta-adrenergic receptors.
Two types for antibodies have been raised against the beta-adrenergic receptor: either by injection of highly purified receptor from turkey erythrocytes or by injection of anticatecholamine ligand antibodies, and induction of anti-idiotypic antibodies. Our data illustrate the interactions of the beta-adrenergic receptor with these polyclonal antibodies. Preliminary results with monoclonal antibodies are also described. The redistribution of beta-receptors on intact cells is visualized by the use of fluorescent antibodies. Immunoprecipitation of radioiodinated receptor by the antireceptor antibodies yields a single major 60,000 MW component.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000917:Antibody Formation; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D011943:Receptors, Adrenergic, beta / Q000276:immunology*; D014422:Turkeys
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011943:Receptors, Adrenergic, beta; D000262:Adenylyl Cyclases