Kinetic analysis in cell culture of the reversal of antiherpes activity of nucleoside analogs by thymidine.
The inhibition of herpes simplex virus type 1 plaque formation by acyclovir, bromovinyldeoxyuridine, 9-(3,4-dihydroxybutyl)guanine, and 9-(4-hydroxybutyl)guanine at different thymidine concentrations was analyzed in Lineweaver-Burk plots. Linear competitive patterns between thymidine and the nucleoside analogs were observed for the inhibition of herpes simplex virus type 1 plaque formation. A new constant, the reversal constant Kr, was introduced to describe the sensitivity in cell culture of an antiviral drug to the reversal of its viral activity by a metabolite (e.g., thymidine).
D000818:Animals; D000998:Antiviral Agents / Q000037:antagonists & inhibitors*; D002522:Chlorocebus aethiops; D006801:Humans; D007668:Kidney; D007700:Kinetics; D009705:Nucleosides / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D018139:Simplexvirus / Q000187:drug effects*; D013936:Thymidine / Q000494:pharmacology*; D014776:Virus Cultivation
D000998:Antiviral Agents; D009705:Nucleosides; D013936:Thymidine