The dibromothymoquinone effect on membrane potential generation in Rhodospirillum rubrum chromatophores.
2,5-Dibromo-3-methyl-6-isopropyl benzoquinone (DBMIB) inhibits the light-dependent membrane potential generation in Rhodospirillum rubrum chromatophores. The inhibition is relieved by electron donors and is obviously due to oxidation of the photosynthetic electron transfer chain components. In addition, high DBMIB concentrations elicit another effect probably caused by disruption of quinone functions in chromatophores. However, in quinone-depleted chromatophores and proteoliposomes containing the P-870 reaction center and light-harvesting antenna complexes, DBMIB stimulates membrane potential generation in the light, probably restoring some of the quinone-dependent processes in the membrane. DBMIB inhibits the inorganic pyrophosphate- and ATP-induced membrane potential generation in chromatophores.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D020130:Bacterial Chromatophores / Q000378:metabolism*; D003991:Dibromothymoquinone / Q000494:pharmacology*; D011756:Diphosphates / Q000494:pharmacology; D004579:Electron Transport; D008027:Light; D008564:Membrane Potentials / Q000187:drug effects / Q000528:radiation effects; D010788:Photosynthesis / Q000187:drug effects; D011809:Quinones / Q000378:metabolism / Q000494:pharmacology*; D012247:Rhodospirillum rubrum / Q000378:metabolism*
D011756:Diphosphates; D011809:Quinones; D003991:Dibromothymoquinone; D000968:Antimycin A; D000255:Adenosine Triphosphate