Interactions of chrysotile asbestos with erythrocyte membranes.
Chrysotile asbestos causes lysis of red blood cells. It has been proposed that the mechanism of hemolysis is mediated through interactions between asbestos and cell membrane glycoproteins. Our studies support this concept and the following results are reported. Electron microscopy shows that asbestos fibers distort red blood cells and bind to cell membranes which may become wrapped around the fibers. This reaction is prevented by pretreatment of the cells with neuraminidase. The distribution of lectins which bind to membrane glycoproteins is altered by treating the cells with asbestos. Cell distortion and membrane deformation consequent to asbestos treatment correlate with a clear increase in the ratio of intracellular Na+:K+ ions.
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017632:Asbestos, Serpentine; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D006023:Glycoproteins / Q000097:blood; D006046:Gold; D006386:Hemagglutination Tests; D006737:Horseshoe Crabs / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009439:Neuraminidase / Q000494:pharmacology; D011188:Potassium / Q000097:blood; D051381:Rats; D012964:Sodium / Q000097:blood; D014909:Wheat Germ Agglutinins
D017632:Asbestos, Serpentine; D006023:Glycoproteins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D001194:Asbestos; D006046:Gold; D012964:Sodium; D009439:Neuraminidase; D011188:Potassium