Erythrocyte ouabain binding capacity as a possible cellular index of hyperthyroid status.
The maximum ouabain binding capacity in erythrocytes from 8 normal subjects and 14 patients with hyperthyroidism was assessed by measuring [3H]-ouabain binding. The mean value for maximum ouabain binding capacity was significantly lower in the patients than in normals (0.422 +/- 0.084 vs 0.671 +/- 0.095 pmol per 10(9) cells, p less than 0.001). Furthermore, a close inverse correlation was found between the ouabain binding capacity and serum T3 (r = 0.766; p less than 0.01) or T4 (r = -0.870; p less than 0.001) levels. These results suggest that the maximum ouabain binding capacity in erythrocytes may provide a useful index of the peripheral effect of thyroid hormone.
D000328:Adult; D002784:Cholesterol / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D006980:Hyperthyroidism / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010042:Ouabain / Q000097:blood*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D013963:Thyroid Hormones / Q000097:blood
D013963:Thyroid Hormones; D010042:Ouabain; D002784:Cholesterol; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase