Three types of acetylcholine-induced single channel currents in clonal rat pheochromocytoma cells.
Single channel currents induced by acetylcholine were recorded from nerve growth factor-differentiated clonal rat pheochromocytoma (PC12) cells by using the patch-clamp technique. Inward current amplitudes were heterogeneous and could be related to three classes of unitary current. The three current-voltage relations were linear for patch membrane potentials ranging from -50 mV to -120 mV. At 22 degrees C, conductances of 22 pS, 31 pS and 39 pS were obtained. The extrapolated reversal potential for the channels was 0 mV and the mean open time approximately 10-20 ms. These results may indicate that PC12 cells possess three types of acetylcholine receptor channels.
D000109:Acetylcholine / Q000494:pharmacology*; D000320:Adrenergic Fibers / Q000187:drug effects*; D000818:Animals; D002999:Clone Cells; D005728:Ganglia, Sympathetic / Q000187:drug effects*; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D008959:Models, Neurological; D009474:Neurons / Q000187:drug effects; D010673:Pheochromocytoma; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects*
D007473:Ion Channels; D000109:Acetylcholine