Lactoferrin-catalysed hydroxyl radical production. Additional requirement for a chelating agent.
The ability of lactoferrin to catalyse hydroxyl radical production was determined by measuring ethylene production from methional (2-amino-4-methylthiobutyraldehyde) or 4-methylthio-2-oxobutyrate. Lactoferrin, isolated from human milk and saturated by adding the exact equivalents of Fe3+-nitrilotriacetic acid and dialysing, give little if any catalysis of the reaction between H2O2 and either O2-. or ascorbic acid at either pH 7.4 or pH 5.0. However, in the presence of chelating agents such as EDTA or nitrilotriacetic acid that can complex with lactoferrin, hydroxyl radical production by both mechanisms was observed.
D000447:Aldehydes / Q000378:metabolism; D002614:Chelating Agents / Q000378:metabolism*; D004492:Edetic Acid / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis; D005290:Ferric Compounds / Q000378:metabolism; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D007781:Lactoferrin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007782:Lactoglobulins / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism
D000447:Aldehydes; D002614:Chelating Agents; D005030:Ethylenes; D005290:Ferric Compounds; D005609:Free Radicals; D006878:Hydroxides; D007782:Lactoglobulins; C008390:methional; D017665:Hydroxyl Radical; C014632:2-keto-4-methylthiobutyric acid; D004492:Edetic Acid; D008715:Methionine; D006861:Hydrogen Peroxide; D007781:Lactoferrin; C019179:Fe(III)-EDTA