P85gag-mos encoded by ts110 Moloney murine sarcoma virus: rapid at the restrictive temperature.
The stability of P85gag-mos, encoded by ts110 Moloney murine sarcoma virus, was studied. P85gag-mos was found to turn over more rapidly at 39 degrees C than 33 degrees C in two different cell lines. One of these is a non-producer cell clone (6m2) which appears only to express P85 at 33 degrees C. The other cell line (206-2IC) produces P85gag-mos at both 33 degrees C and 39 degrees C, although in lower amounts at the higher temperature. Both cell lines exhibit the normal morphological phenotype following a shift to the restrictive temperature. These data suggest that the presence of P85gag-mos alone is insufficient to cause transformation, and that some function(s) associated with the molecule may be heat-labile.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004355:Drug Stability; D015683:Gene Products, gag; D005815:Genetic Code; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics*; D014776:Virus Cultivation
D015683:Gene Products, gag; D014764:Viral Proteins