Use of enzyme linked immunosorbent assay (ELISA) in the quantitation of Clostridium perfringens type A enterotoxin and antienterotoxin antibodies.
ELISA procedure for quantitation of enterotoxin of Clostridium perfringens type A was worked out. It was possible to detect as little as 0.0022 microgramme of enterotoxin. When applied to quantitate the enterotoxin in laboratory contaminated faeces and cooked minced beef, ELISA detected as little as 0.024 microgramme of enterotoxin. ELISA procedure for determination of titre of antibodies to enterotoxin was also developed using hyperimmunised rabbit and sheep sera. It was possible to use this procedure in sero-survey.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle; D003016:Clostridium perfringens / Q000276:immunology*; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000032:analysis; D006801:Humans; D008460:Meat / Q000032:analysis; D011817:Rabbits; D012756:Sheep
D000907:Antibodies, Bacterial; D004768:Enterotoxins; C035598:enterotoxin, Clostridium