Stimulation of prothrombinase activity of platelets and erythrocytes by sub-lytic treatment with phospholipase C from Clostridium welchii.
Treatment of platelets or red cells with small amounts of phospholipase C from Clostridium welchii enables both cells, prior to the onset of lysis, to stimulate prothrombin conversion by coagulation factor Xa and Va in the presence of calcium. Phospholipase C treatment of both cells also exposes significant amounts of phosphatidylserine at the outer surface. The level of phosphatidic acid formed from diglycerides produced by phospholipase C action, is similar to that formed in activated platelets upon triggering the phosphatidylinositol cycle. A possible involvement of this cycle to activate platelets to become more procoagulant is discussed.
D001792:Blood Platelets / Q000201:enzymology*; D003016:Clostridium perfringens / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010740:Phospholipases / Q000494:pharmacology*; D013925:Thromboplastin / Q000378:metabolism*; D010738:Type C Phospholipases / Q000494:pharmacology*
D013925:Thromboplastin; D010740:Phospholipases; D010738:Type C Phospholipases