Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.
The 5' control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon-alpha. This PGK-interferon fusion was then introduced into yeast on a high copy number 2mu-based plasmid vector. Strains containing this plasmid produced a PGK-interferon-alpha fusion protein as 1-2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon-alpha per litre of batch culture.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D006801:Humans; D007370:Interferon Type I / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D007370:Interferon Type I; D010735:Phosphoglycerate Kinase; D004262:DNA Restriction Enzymes