Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).
Chromatin was reconstituted from core histones and recombinant plasmid DNAs carrying poly(dA) . poly(dT) inserts of various lengths. A 97-bp insert was found to occupy discrete and regularly-spaced positions on the edges of the nucleosome. This insert cannot, however, be entirely included due to a block in the center of the particle. In contrast, nucleosomes reconstitute on a shorter 20-bp insert. In this case, the insert shows a marked preference for the edges of the particle. Possible structural and physiological implications of these observations are discussed.
D000818:Animals; D002843:Chromatin / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D006657:Histones / Q000378:metabolism; D008297:Male; D009707:Nucleosomes / Q000032:analysis*; D010957:Plasmids; D011067:Poly dA-dT / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D004270:DNA, Circular; D004274:DNA, Recombinant; D006657:Histones; D009707:Nucleosomes; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases