Characteristics of insulin and epidermal growth factor stimulation of receptor autophosphorylation in detergent extracts of rat liver and transplantable rat hepatomas.
Insulin and epidermal growth factor (EGF) share a number of metabolic actions, including stimulation of protein synthesis and growth in certain tissues, and activation of apparent receptor tyrosine kinase activities. We have shown that insulin and EGF promote the phosphorylation of a number of intracellular proteins in common, suggesting that some of the shared metabolic actions of these hormones might be due to shared effects on protein kinases or phosphatases. We, therefore, compared the effects of these hormones on their respective membrane receptor autophosphorylation reactions in detergent extracts prepared from rat liver microsomes. Under appropriate conditions, ligand-promoted receptor autophosphorylation could be observed without further receptor purification. Insulin- and EGF-stimulated receptor autophosphorylation exhibited differing requirements for divalent cations and optimum ATP concentrations, and were enhanced by detergent extraction of the membranes. Insulin-promoted receptor autophosphorylation occurred on a tyrosine residue. In liver microsomal extracts prepared from rats exposed to various dietary conditions, insulin-stimulated receptor autophosphorylation was enhanced in extracts prepared from starved or diabetic animals when compared to those prepared from fed or fasted-refed animals. Experiments with extracts from several transplantable rat hepatomas of varying degrees of differentiation indicated that both insulin binding and insulin-stimulated receptor autophosphorylation were remarkably preserved in all tumors; in contrast, EGF binding and EGF-induced receptor autophosphorylation were diminished or absent in all tumors studied. These studies suggest that the relationship between insulin-mediated receptor phosphorylation and subsequent metabolic events might be profitably studied in these tissues.
D000818:Animals; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors; D007328:Insulin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*
D007328:Insulin; D011956:Receptors, Cell Surface; C008147:insulin, iodo-; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D011972:Receptor, Insulin