title: Stoichiometric use of the transposase of bacteriophage Mu.
abstract: The transposase of bacteriophage Mu (gene A protein) mediates the coupled replication and integration processes that constitute transposition during the lytic cycle. Our previous results showed that the activity of the A protein is unstable, as its continued synthesis is required to maintain Mu DNA replication throughout the lytic cycle. We present here the results of experiments in which the A protein is used stoichiometrically and must be synthesized de novo for each round of Mu DNA replication. Induction of a Mu lysogen in the absence of DNA replication allows accumulation of potential for a single round of Mu DNA replication. Once achieved, this potential is stable even in the absence of further protein synthesis. Release of inhibition of DNA replication leads to a single semi-conservative replicative transposition event, followed by later rounds only if additional synthesis of the A protein is allowed.
mesh_terms: D010583:Bacteriophage mu / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D004261:DNA Replication; D008242:Lysogeny; D009713:Nucleotidyltransferases / Q000378:metabolism*; D019895:Transposases; D014764:Viral Proteins / Q000378:metabolism*; D014775:Virus Activation; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D009713:Nucleotidyltransferases; D019895:Transposases