title: The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis.
abstract: Nucleotide sequences of the homologous tetracycline resistance (tet) determinants of plasmid RP1 and transposon Tn1721 have been determined. Two open reading frames of divergent polarity have been assigned to a regulatory gene (tetR) and a gene encoding a resistance protein (tetA). The intercistronic region contains appropriate regulatory and transcription signals. The tetR gene can code for a protein of 216 amino acids (deduced mol.wt. 23,288) and the tetA gene for a protein of 399 amino acids (deduced mol. wt. 42,205). Based on the deduced amino acid sequence, the tetA proteins of RP1/Tn1721 are 78% homologous with that of pBR322 and 45% homologous with that of Tn10. We conclude that a single tetA gene mediates resistance in each of these tet determinants.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements / Q000187:drug effects*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial / Q000187:drug effects*; D005809:Genes, Regulator / Q000187:drug effects*; D010957:Plasmids / Q000187:drug effects*; D013752:Tetracycline / Q000494:pharmacology*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D013752:Tetracycline