Study in pig coronary smooth muscle cell subcellular fractions of the activity of various enzymes involved in lipid metabolism and of the beta-receptor adenylate cyclase couple.
Pig coronary smooth muscle cells contain a highly active fatty acid beta-oxidation system as well as significant activities of other enzymes involved in lipid metabolism, i.e. palmitoyl-CoA synthetase, glycerophosphate acyl transferase, acetyl-CoA carboxylase and fatty acid synthetase. Purified plasma membranes of pig coronary smooth muscle cells contain stereospecific (-)-[3H]dihydroalprenolol binding sites of high affinity (Kd = 5.4 nM) and characteristic of the beta 1 subtype. They also contain an adenylate cyclase activity which is stimulated by 5'-guanylyl-imidodiphosphate (Gpp(NH)p) and, to a lesser degree, by (-)-isoproterenol.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003331:Coronary Vessels / Q000201:enzymology*; D004082:Dihydroalprenolol; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D050356:Lipid Metabolism; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology; D013552:Swine
D011943:Receptors, Adrenergic, beta; D006165:Guanylyl Imidodiphosphate; D004082:Dihydroalprenolol; D012969:Sodium Fluoride; D000262:Adenylyl Cyclases