99mTc-labeled nucleotides as tumor-seeking radiodiagnostic agents.
Several lines of human tumor cells in monolayer and soft agar cultures allow permeation of low levels of adenine nucleotides through their plasma membranes, while, in general, untransformed cells do not incorporate adenine nucleotides into their cellular pools without prior degradation of the nucleotides to adenosine. This study determined the uptake of 99mTc-radiolabeled chelated forms of adenine nucleotides, 99mTc-Ap4A (diadenosine 5',5"',P1,P4-tetraphosphate) and 99mTc-ATP chelates as radiodiagnostic agents suitable for the in vivo detection of tumors by radionuclide imaging. Biodistribution studies revealed that Ap4A accumulated preferentially in RT-24 tumors implanted in rats and that V2 carcinoma implanted in rabbits could be readily visualized by in vivo imaging. The biodistribution at various time points showed increased tumor-to-muscle ratios after 99mTc-Ap4A or 99mTc-ATP injections when compared with a nonspecific marker of the extracellular fluid space, 99mTc-labeled diethylenetriaminepentaacetic acid and with an agent known to localize in some tumors, 67Ga-labeled citrate. Studies of ectoenzymatic activities of virus-transformed animal cells and their untransformed counterparts in monolayer cultures showed marked decreases in the ectoenzymatic activities that degrade Ap4A in the transformed cells. Incorporation of en bloc [3H, 32P]Ap4A into cellular acid-soluble nucleotide pools of certain transformed cells was observed. Normal untransformed cells incorporated the radioactive label only by prior degradation to [3H]adenosine and 32Pi.
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging*; D002478:Cells, Cultured; D015226:Dinucleoside Phosphates; D005910:Glioma / Q000000981:diagnostic imaging*; D006801:Humans; D007668:Kidney / Q000000981:diagnostic imaging; D007700:Kinetics; D008113:Liver Neoplasms / Q000000981:diagnostic imaging*; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000000981:diagnostic imaging; D015609:Organotechnetium Compounds; D011877:Radionuclide Imaging; D051381:Rats; D013667:Technetium; D014018:Tissue Distribution
D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D015609:Organotechnetium Compounds; C040736:technetium Tc 99m adenosine triphosphate; C040737:technetium Tc 99m diadenosine tetraphosphate; D013667:Technetium; D000255:Adenosine Triphosphate