A simple quantitative protein A micro-immunoprecipitation method; assay of antibodies to the N and H antigens of poliovirus.
Staphylococcus aureus (Cowan strain I) was used to absorb immune complexes from antiserum to poliovirus to which labeled N or H poliovirus antigens had been added, and the radioactivity in the pelleted organisms and in the supernatant was measured. Excellent agreement was obtained between values calculated separately from the pellet and supernatant readings, validating the use of supernatant measurements from a microtitration plate method.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000276:immunology*; D011232:Chemical Precipitation; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology; D017955:Poliovirus / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013205:Staphylococcal Protein A / Q000494:pharmacology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D013205:Staphylococcal Protein A