Comparison of six methods for detecting human rotavirus in stools.
The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex , 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost , 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex .
D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007841:Latex Fixation Tests; D008854:Microscopy, Electron; D012401:Rotavirus / Q000302:isolation & purification*