[Detection of the biological activity of SV40 virus DNA after injection into the nuclei of simian cells].
EV40 DNA closed superhelical form was introduced into cell nuclei by means of direct microinjection and expression of the viral genome followed. The expression of viral T-antigen was observed 40 hours after the injection, and SV40-specific effect developed on the fifth day. Using the method discussed, the infection dose of SV40 DNA in a permissive cell culture system was shown to be approximately one molecule per cell nucleus.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002472:Cell Transformation, Viral; D004278:DNA, Superhelical / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D000882:Haplorhini; D007700:Kinetics; D008845:Microinjections; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology
D000957:Antigens, Viral, Tumor; D004278:DNA, Superhelical; D004279:DNA, Viral
Vyiavlenie biologicheskoĭ aktivnosti DNK virusa SV 40 posle in"ektsii v iadra kletok obez'ian.