title: Is the mitochondrial precursor protein apocytochrome c able to pass a lipid barrier?
abstract: To obtain insight into the role of lipids in the translocation of extramitochondrially synthesized proteins, we studied the ability of apocytochrome c to pass lipid bilayers. With polyacrylamide gel electrophoresis, the digestion of externally added apocytochrome c by trypsin, enclosed in lipid vesicles, was followed. The experiments demonstrate that apocytochrome c is able to pass a lipid barrier and this process shows both a lipid- and protein specificity. The most probable molecular mechanisms involved in this phenomenon are discussed.
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D045304:Cytochromes c; D004591:Electrophoresis, Polyacrylamide Gel; D006736:Horses; D008563:Membrane Lipids / Q000378:metabolism*; D010718:Phosphatidylserines; D014357:Trypsin / Q000378:metabolism
chemical_list: D001059:Apoproteins; D003574:Cytochrome c Group; D008563:Membrane Lipids; D010718:Phosphatidylserines; D045304:Cytochromes c; D014357:Trypsin