Influence of chronic ethanol treatment on alpha1-adrenergic and vasopressin receptor-stimulated phosphatidylinositol synthesis in isolated rat hepatocytes.
Adult male rats were given nutritionally balanced high fat (34% corn oil) diets containing either ethanol or isocalorically substituted sucrose for 4-5 weeks. Phosphatidylinositol-phosphatidylserine, phosphatidylethanolamine and phosphatidylcholine contents of whole hepatocytes were not altered in ethanol-treated rats. However, vasopressin and alpha1-adrenergic stimulation of [32P]-incorporation into phosphatidylinositol of isolated hepatocytes from ethanol-treated rats was increased substantially compared to that of controls. Yet, chronic ethanol treatment had no effect on hepatic alpha1 receptor density or affinity as measured by [3H]prazosin specific binding. These results suggest that the supersensitive phosphatidylinositol response to this alpha1 agonist observed in hepatocytes from ethanol-treated rats occurred distal to cell surface receptors and may be similar for vasopressin.
D000437:Alcoholism / Q000503:physiopathology*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010761:Phosphorus Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000502:physiology*; D017483:Receptors, Vasopressin
D004041:Dietary Fats; D008055:Lipids; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D011942:Receptors, Adrenergic, alpha; D011956:Receptors, Cell Surface; D017483:Receptors, Vasopressin; D004837:Epinephrine