title: Beta-endorphin-like immunoreactivity in extracts of the fetal bovine pancreas. Column chromatographic characterizations of a high-molecular-weight immunoreactive species.
abstract: Acid-ethanol extracts of fetal bovine pancrease were examined for the presence of beta-endorphin-like immunoreactivity. Gel-filtration analyses revealed the presence of a major large-molecular-weight beta-endorphin immunoreactive species of approximately 20K delta. This molecular form maintained its size upon resubmission to gel filtration in the presence of 6 M guanidine hydrochloride, separated from the bulk of the glucagon immunoreactivity upon ion-exchange chromatography, showed proportional dilution in the beta-endorphin radioimmunoassay, and interacted in a biospecific manner with Concanavalin-A-Sepharose.
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A; D004723:Endorphins / Q000032:analysis* / Q000276:immunology; D005333:Fetus / Q000378:metabolism; D008970:Molecular Weight; D010179:Pancreas / Q000032:analysis*; D011863:Radioimmunoassay; D001615:beta-Endorphin
chemical_list: D004723:Endorphins; D003208:Concanavalin A; D001615:beta-Endorphin