Cell cycle requisite for vesicular stomatitis virus replication in T lymphocytes activated with concanavalin A.
The relationship between the development of virus plaque forming cells (V-PFC) and the blastogenic response of T cells activated with concanavalin A (Con A) was investigated in connection with the cell cycle using a modified virus plaque assay (VPA) which facilitated the quantitative detection of T lymphoblasts with low susceptibility to infection by vesicular stomatitis virus (vsv). The generation of V-PFC markedly decreased when DNA synthesis inhibitors such as thymidine, hydroxyurea and cytosine arabinoside (Ara C) were added to spleen cell cultures stimulated with Con A, while the addition of a mitotic inhibitor, Colcemid, to the cultures caused only partial reduction of V-PFC development. These results indicate that the S to G2 phases of the first cell cycle are essential for vsv-replication or V-PFC development in Con A activated T cells. However, vsv-replication in the activated T cells does not depend on the initial G1 phase during the blastogenic response since the morphological blast formation of activated T cells is not suppressed by treatment with DNA synthesis inhibitors.
D000818:Animals; D002453:Cell Cycle; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D003208:Concanavalin A