title: Comparison of pyrimidine 5'nucleotidase activity in erythrocytes of sheep, dogs, cats, horses, calves, and Mongolian gerbils.
abstract: Pyrimidine 5'nucleotidase (P5N) activities of erythrocytes for Mongolian gerbils, cats, dogs, sheep, horses, and calves were measured, using a radiometric technique with [14C]cytidine monophosphate as the substrate. Erythrocytes of gerbils had the highest activity [1,177.1 +/- 133.6 mU/g of hemoglobin (Hb)]. Feline erythrocytes had 327.4 +/- 204.4 mU/g of Hb. Canine erythrocytes had 148.0 +/- 19.8 mU/g of Hb. Ovine erythrocytes (44.3 +/- 20.9 mU/g of Hb), equine erythrocytes (30.0 +/- 15.9 mU/g of Hb), and bovine erythrocytes (14.1 +/- 6.9) had relatively low P5N activity. The P5N activity was approximately proportional to the reticulocyte percentage of and inversely proportional to the mean erythrocyte life span in these species.
mesh_terms: D015720:5'-Nucleotidase; D000818:Animals; D002415:Cats / Q000097:blood*; D002417:Cattle / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004905:Erythrocyte Aging; D004906:Erythrocyte Count / Q000662:veterinary; D004912:Erythrocytes / Q000201:enzymology*; D005849:Gerbillinae / Q000097:blood*; D006736:Horses / Q000097:blood*; D009708:Nucleotidases / Q000097:blood*; D012156:Reticulocytes / Q000166:cytology; D012756:Sheep / Q000097:blood*; D013045:Species Specificity
chemical_list: D009708:Nucleotidases; D015720:5'-Nucleotidase