Gene transfer into mammalian cells.
The introduction of purified genes into animal cells allows the study of gene expression and regulation and the establishment of genetically modified cell lines. Several methods are available of transferring DNA into cells of higher eukaryotes: transfection with calcium phosphate, fusion of cells with liposomes or bacterial protoplasts, and micro-injection of DNA. Transient and/or permanent expression of foreign genes can be obtained in biochemically transformed cells.
D000818:Animals; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D005822:Genetic Vectors; D008322:Mammals; D008722:Methods; D014170:Transformation, Genetic
D004274:DNA, Recombinant