Metabolic activation of cytochrome P-450/P-448 in the yeast Saccharomyces cerevisiae.
The strains D6 and JD1 of the yeast Saccharomyces cerevisiae were used to assay the genetic activity of several compounds, benzo[a]pyrene, 15,16-dihydro-11-methyl-cyclopenta[a]phenanthren-17-one, 2-naphthylamine and cyclophosphamide, which require metabolic activation by cytochromes P-450 and P-448 to produce genetically active chemical species. Cells from both strains were harvested from cultures grown in low concentrations of glucose and switched to growth in high glucose containing media. Treatments under these conditions resulted in increased sensitivity of the test systems without the presence of an exogenous S9 mix and the presence of S9 was found not to enhance this sensitivity. The yeasts used under these treatment conditions showed a P-450/P-448 type metabolism.
D015081:2-Naphthylamine / Q000378:metabolism; D000782:Aneuploidy / Q000187:drug effects; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism; D001711:Biotransformation; D003520:Cyclophosphamide / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003580:Cytochromes / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006067:Gonanes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D001580:Benzopyrenes; D003580:Cytochromes; D004272:DNA, Mitochondrial; D006067:Gonanes; D001564:Benzo(a)pyrene; C005765:15,16-dihydro-11-methylcyclopenta(a)phenanthren-17-one; D003520:Cyclophosphamide; D003577:Cytochrome P-450 Enzyme System; D015081:2-Naphthylamine; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D005947:Glucose