Evaluation of a commercial enzyme immunoassay kit for rotavirus detection.
A commercial enzyme immunoassay kit (Rotazyme) was compared to electron microscopy for the detection of rotavirus in stool specimens collected during diarrhea outbreaks at day care centers in Houston. EIA was more sensitive than EM and detected SA-11 rotavirus which titered 2.0 X 10(3) PFU/ml.
D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008854:Microscopy, Electron; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus / Q000032:analysis*; D012400:Rotavirus Infections / Q000382:microbiology
D011933:Reagent Kits, Diagnostic