Inhibition of iodide accumulation by perchlorate and thiocyanate in a model of the thyroid iodide transport system.
The manner of inhibition of thyroid I- accumulation by perchlorate (CIO4-) and thiocyanate (SCN-) was studied using a newly developed biological model of the I- transport system. CIO4- inhibited I- accumulation in phospholipid vesicles made from thyroid plasma membrane and soybean phospholipids by decreasing Na+-dependent I- influx. The anion did not at all induce I- leakage from the vesicles. On the basis of Lineweaver-Burk plot analysis, it did not change Vmax for I- concentration. These results suggest that CIO4- is a competitive inhibitor of thyroid I- transport. In contrast, SCN- did increase I- leakage from the phospholipid vesicles to diminish I- accumulation. This anion might cause only slight depression of Na+-dependent I- entry, if any. The results do not support the idea that SCN- may be a competitive inhibitor, in spite of the fact that the anion did not change Vmax for I- transport on the basis of Lineweaver-Burk plot analysis.
D002462:Cell Membrane / Q000378:metabolism; D007454:Iodides / Q000378:metabolism*; D008954:Models, Biological; D010472:Perchlorates / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D013861:Thiocyanates / Q000494:pharmacology*; D013961:Thyroid Gland / Q000378:metabolism*
D007454:Iodides; D010472:Perchlorates; D010743:Phospholipids; D013861:Thiocyanates