Relationship of simian virus 40 tumor antigens to virus-induced mutagenesis.
We analyzed the mutation frequency to 8-azaguanine (8AZ) resistance in rat FR3T3 cells acutely infected with simian virus 40 wild type and tsA and early deletion mutants and in a series of temperature-sensitive (N) and temperature-insensitive (A) transformants derived from Chinese hamster lung (CHL) cells. Upon acute infection, the frequency of mutation to 8AZ resistance was raised at most by two- to eightfold over the spontaneous frequency, and it was independent of the presence of a functional 90,000-molecular-weight T antigen or 20,000-molecular-weight t antigen or both. Similarly, in the stable transformants of CHL cells, no correlation was found between functional T antigens and mutation to 8AZ resistance. It therefore seems unlikely that simian virus 40-induced transformation results from any mutagenic activity of this virus.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D001375:Azaguanine / Q000494:pharmacology; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004351:Drug Resistance; D051379:Mice; D009154:Mutation; D013539:Simian virus 40 / Q000276:immunology*
D000956:Antigens, Viral; D001375:Azaguanine