Activity of two polyene and two imidazole antimicrobics on Candida albicans in human fibrin clots.
The vegetations of infective fungal endocarditis were simulated by preparing human fibrin clots containing 10(5) CFU of Candida albicans per clot. Clots were incubated in human serum without drugs (controls) or with equimolar concentrations (2.5 to 20 nmol/ml) of four antifungal antimicrobics: AMB and miconazole (both colloidal suspensions) and DAB and KET (both molecularly dispersed). Over a period of 4 days of incubation, clots were resuspended in freshly prepared serum-antimicrobic every 12 hr. Cohorts of control and drug-exposed clots were cultured quantitatively at 24 hr intervals. Neither imidazole at any concentration tested prevented growth of C. albicans to an extent that was significantly different from growth in the control clots. Both polyenes reduced the CFU/clot, with AMB significantly more active than DAB.
D000666:Amphotericin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D000935:Antifungal Agents; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media; D005337:Fibrin; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D007654:Ketoconazole; D008825:Miconazole / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011090:Polyenes / Q000494:pharmacology
D000935:Antifungal Agents; D003470:Culture Media; D007093:Imidazoles; D010879:Piperazines; D011090:Polyenes; D008825:Miconazole; D000666:Amphotericin B; C038420:amphotericin B, N-(N'-(3-dimethylaminopropyl)-N''-ethylguanyl)-; D005337:Fibrin; D007654:Ketoconazole