Ultrastructural changes in differentiating neuroblastoma x glioma hybrid cells.
The ultrastructure of differentiating neuroblastoma x glioma hybrid cells NG108-15 was observed. Cells cultured in growth medium showed undifferentiated features, while cells treated with dBcAMP became round and large, and extended thick long neurites. After 1 week in culture, cells showed features similar to those of normal neurons. The dense cored vesicles with diameters ranging from 60 to 170 nm were observed in differentiated NG108-15 cells, but clear vesicles were usually rare. However, in the case of co-culture with striated myotubes, clusters of clear vesicles appeared in the neurites and terminals. The timecourse of the differentiation process was correlated with results obtained by the electrophysiology and freeze-fracture.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D003470:Culture Media; D005614:Freeze Fracturing; D005910:Glioma / Q000648:ultrastructure*; D006822:Hybrid Cells / Q000648:ultrastructure*; D066298:In Vitro Techniques; D051379:Mice; D008854:Microscopy, Electron; D009447:Neuroblastoma / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology; D051381:Rats; D013997:Time Factors
D003470:Culture Media; D003994:Bucladesine