Transport and cleavage of bacterial pre-beta-lactamase by mammalian microsomes.
Eucaryotic endoplasmic reticulum and bacterial inner cell membranes use strikingly similar mechanisms to transport proteins from one cellular compartment to another. Using a mammalian linked transcription-translation system, we show here that canine pancreatic microsomes accurately cleave off the signal sequence of beta-lactamase, a secreted bacterial protein. We suggest that the apparent differences between bacterial and eukaryotic protein transport may involve only minor modulations of a profoundly conserved mechanism.
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D004285:Dogs; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D008861:Microsomes / Q000378:metabolism*; D010179:Pancreas / Q000648:ultrastructure; D010957:Plasmids; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D004792:Enzyme Precursors; C037640:pre-beta-lactamase; D001618:beta-Lactamases