[Transport of Rb+ via activated sodium channels of clone N 18 phi 1 neuroblastoma cells].
A study was made of the Rb+ transport via activated sodium channels of clone N 18 phi 1 neuroblastoma cells cultured in the Eagle medium with 10% bovine serum. The time of population doubling was about 10 h. The cell differentiation was induced by adding bromdeoxyuridine in a concentration of 1-4 10(-5) M. The cells contained 172 +/- 12 and 340 +/- 35 micrograms of protein per 10(6) cells at the logarithmic growth phase and in differentiated state, respectively. It is shown that veratrin produced a 1.3-fold increase in the rate of 86Rb+ removal from undifferentiated cells and 2.5-fold increase in that from differentiated cells. Tetrodotoxin removed completely the effect of veratrin. A conclusion is made on the presence of a new clone of fast sodium channels in cell membranes.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002454:Cell Differentiation; D002460:Cell Line; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D007473:Ion Channels; D013779:Tetrodotoxin; D012964:Sodium; D012413:Rubidium
Transport Rb+ cherez aktivirovannye natrievye kanaly kletok neĭroblastomy klona N 18 fi 1.