Allospecific and virus-specific cytolytic T lymphocytes are restricted to the N or C1 domain of H-2 antigens expressed on L cells after DNA-mediated gene transfer.
In order to identify the site(s) on major histocompatibility molecules recognized by cytolytic T lymphocytes (CTLs), the recognition of H-2 antigens expressed when cloned genes are introduced into mouse L cells by DNA-mediated gene transfer has come under investigation. Recently, recombinant H-2 genes have been constructed in vitro from restriction endonuclease fragments of cloned H-2Dd and H-2Ld genes which exchange the N and C1 external domains (exon shuffling). These hybrid H-2 genes direct the synthesis of hybrid H-2 antigens when introduced into L cells by DNA-mediated gene transfer. These transformed L cells have been used as target cells to achieve a more precise localization of the sites recognized by allospecific and virus-specific CTLs. CTL systems were chosen that allow one to probe allospecific Ld or Dd recognition or virus-restricted Ld or Dd recognition. Using this approach we were able to map essential CTL recognition sites to the N and C1 domains of class 1 molecules.
D000818:Animals; D001665:Binding Sites; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D005260:Female; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007739:L Cells / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus
D006183:H-2 Antigens; D004247:DNA