A molecular characterization of H. influenzae plasmid pVe445: a comparison with N. gonorrhoeae plasmids pNG10 and pNG18.
Haemophilus influenzae plasmid pVe445 (4.6 MDa) has been compared with Neisseria gonorrhoeae plasmids pNG18 (3.3 MDa) and pNG10 (4.6 MDa) by heteroduplex and restriction enzyme analysis. This study demonstrates that pVe445 differs from pNG18 only that it carries an additional 1.3-MDa DNA fragment between coordinates 0.9 and 2.2. Heteroduplex studies demonstrated that pNG10 and pVe445 are indistinguishable whereas restriction endonuclease analysis indicated minor differences in the distribution of AluI sites.
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D006193:Haemophilus influenzae / Q000235:genetics*; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010957:Plasmids; D013045:Species Specificity
D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes