Radioimmunoassay of oxidized calmodulin in chloramine-T treated tissue samples.
We found that a short incubation of calmodulin with chloramine-T alters its antigenic structure. Antisera elicited by performic acid oxidized calmodulin only bind with chloramine-T treated calmodulin. Based on this finding we have developed an assay for the determination of calmodulin in small tissue samples. Tissue homogenates are incubated with 10 mmol/l chloramine-T before calmodulin quantification. Immune complexes are isolated by sodium sulphate precipitation. [125I]calmodulin is used as tracer.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001921:Brain / Q000201:enzymology; D002147:Calmodulin / Q000032:analysis*; D002700:Chloramines; D008099:Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D010320:Parvalbumins / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D014105:Tosyl Compounds; D014336:Troponin / Q000032:analysis
D002147:Calmodulin; D002700:Chloramines; D010320:Parvalbumins; D014105:Tosyl Compounds; D014336:Troponin; C016300:chloramine-T; D015105:3',5'-Cyclic-AMP Phosphodiesterases