Mononuclear cell factors that inhibit fibroblast collagen synthesis. II. Properties of the factors.
Supernatants harvested from peripheral blood mononuclear cells (PBMC) incubated either with the non-specific mitogen phytohaemagglutinin-P (PHA) or with the specific antigen tuberculin purified protein derivative for 72 h decreased collagen synthesis by dermal fibroblasts. PHA-induced mononuclear cell factors (PHA-MCF) responsible for decreases in collagen synthesis by dermal fibroblasts were localized by column chromatography on Sephacryl S-200 to fractions of 30,000-60,000 daltons. Proteolytic enzymes destroyed the activity of PHA-MCF, but after incubation with neuraminidase some activity of these factors remained. The activity of PHA-MCF was not inhibited by incubation with the monosaccharides L-fucose, L-rhamnose, N-acetylglucosamine, and alpha-methyl-D-mannoside but was partially destroyed by heating at 80 degrees C for 10 min. The factors were not mitogenic to PBMC. These factors did not appear to resemble any previously characterized factors produced by non-adherent mononuclear cells. The mechanism by which these factors decreased fibroblast collagen synthesis appeared complex. There was no detectable increase in the release of collagenase by fibroblasts, nor was a cytotoxic effect apparent. Increased PGE2 production by fibroblasts could not be related to the factor-induced decrease in fibroblast collagen synthesis.
D003094:Collagen / Q000096:biosynthesis*; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone; D005347:Fibroblasts / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D003012:Microbial Collagenase / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D008970:Molecular Weight; D009005:Monosaccharides / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011448:Prostaglandin Antagonists / Q000502:physiology; D011458:Prostaglandins E / Q000096:biosynthesis
D006020:Glycopeptides; D008934:Mitogens; D009005:Monosaccharides; D010835:Phytohemagglutinins; D011448:Prostaglandin Antagonists; D011458:Prostaglandins E; C020327:fibroblast proliferation inhibitor; D003094:Collagen; D010447:Peptide Hydrolases; D003012:Microbial Collagenase; D015232:Dinoprostone