title: The solute selectivity of porin pores of Escherichia coli and Salmonella typhimurium.
abstract: The solute selectivity of porin pores of Escherichia coli and Salmonella typhimurium was examined using vesicle membranes reconstituted from phospholipids and purified porin trimers. OmpE- and 34K-porin-pores allowed a preferential diffusion of phosphorylated and/or negatively charged compounds. They allowed the diffusion of positively charged solutes reasonably well. OmpF- and 35K-porin-pores favored the diffusion of positively charged solutes a little more than negatively charged solutes. OmpC- and 36K-porin-pores showed poor diffusibility to all solute tested. B-porins from E. coli B formed highly efficient pores for the diffusion of both positively and negatively charged solutes. On the basis of these observations, possible mechanisms of solute selectivity were discussed.
mesh_terms: D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure*; D007473:Ion Channels / Q000378:metabolism / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010539:Permeability; D018272:Porins; D012486:Salmonella typhimurium / Q000378:metabolism / Q000648:ultrastructure*; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D007473:Ion Channels; D008565:Membrane Proteins; D018272:Porins