Inhibition of colony formation by macrophage committed stem cells during acute inflammation by purified human C-reactive protein (CRP).
Human C-reactive protein (CRP), an acute phase reactant purified from ascites fluids, selectively inhibited in vitro monocyte colony formation by bone marrow granulocyte-monocyte colony-forming cells (GM-CFC) from untreated mice, but not from mice that had undergone an acute systemic inflammatory response of 3-4 days duration. The CRP-susceptible M-CFC accumulated in the peritoneal cavity during the course of an inflammatory stimulus at this site. Aggregated CRP and CRP-complexes were as inhibitory as the monomeric form of purified CRP. Addition of phosphorylcholine (P-C), the major determinant on the C-polysaccharide (CPS) to which CRP binds, did not alter the inhibitory activity of CRP. Treatment of resident peritoneal macrophages with CRP did not lower their ability to serve as a source of CSF for GM-CFC. The results are consistent with a regulatory role for CRP during monocytopoiesis induced by inflammation.
D000818:Animals; D001667:Binding, Competitive; D002097:C-Reactive Protein / Q000008:administration & dosage / Q000378:metabolism / Q000502:physiology*; D002449:Cell Aggregation; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000209:etiology; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D011956:Receptors, Cell Surface / Q000032:analysis; D016184:Receptors, Colony-Stimulating Factor
D003115:Colony-Stimulating Factors; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor; D002097:C-Reactive Protein