Independent segregation of ev 10 and ev 11, genetic loci for spontaneous production of endogenous avian retroviruses.
F1 males produced by crossing Regional Poultry Research Laboratory line 15I4 that carries endogenous viral (ev) genes 1, 6, and 10 and a partially defined gene, ev 11, with line 15B that carries ev 1 and 7 were backcrossed to line 15B females. The DNA of 72 progeny embryos was digested by BamHI and Kpn and hybridization of resulting fragments with 32P-labeled viral RNA showed that ev 6, 10, and 11 were autosomal genes and not closely linked, and confirmed that ev 7 was sex linked. ev 10 and 11 were consistently associated with complete virus production, confirming that the newly defined ev 11 codes for a complete endogenous retrovirus.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000382:microbiology; D003433:Crosses, Genetic; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005260:Female; D005814:Genes, Viral; D008040:Genetic Linkage; D008297:Male; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology
D004247:DNA; D004262:DNA Restriction Enzymes