Interferon production by cultured murine splenocytes in response to influenza virus-infected cells.
Murine splenic lymphocytes cultured for 18 h with either irradiated syngeneic cells infected with influenza A/PR/8/34 virus or irradiated allogeneic cells infected with the same virus release significant quantities of alpha interferon (IFN-alpha) in 24 h. Uninfected allogeneic cells, which are potent stimulators of both lymphocyte proliferation and cytolytic T lymphocytes, failed to induce detectable level of IFN under the same conditions. Cells derived from naive and virus-primed animals gave similar responses to these stimulae. The phenotype of the cells interacting in vitro to these conditions is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D009980:Influenza A virus; D007370:Interferon Type I / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007370:Interferon Type I