Modulation of Na+ transport systems in Wistar rat erythrocytes by excess dietary Na+ intake.
Plasma Na+, erythrocyte Na+ content and the activity of Na+ transport systems of red cells were measured in Wistar rat fed a normal or high Na+ diet. Net Na+ and K+ fluxes of erythrocytes were also measured in the presence of plasma of rats fed with excess Na+. Na+-K+ cotransport and passive Na+ permeability were increased. Erythrocyte Na+ content was increased after 2 months but not after 8 days of high Na+ intake. No significant difference in plasma Na+ and pump activity could be detected after such a diet. No factor acting in Na+ extrusion was found to be present in plasma of salt loaded rats. These results indicate that Na+ intake may modulate Na+ transport systems, namely passive permeability and Na+-K+ cotransport and that increased Na+ erythrocyte content is not a causative factor.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004032:Diet; D004912:Erythrocytes / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000097:blood / Q000494:pharmacology*
D007473:Ion Channels; D002034:Bumetanide; D012964:Sodium; D011188:Potassium