A comparison of Rubazyme-M and MACRIA for the detection of rubella-specific IgM.
One-hundred and eighty-six carefully selected sera were tested for rubella-specific IgM by Rubazyme-M (Abbott Diagnostics) and an M-antibody capture radioimmunoassay (MACRIA). Eleven of these sera were from cases of infectious mononucleosis, six of which gave positive results in MACRIA, while one gave a positive result in Rubazyme-M. Of the remaining 175 sera, 158 gave concordant results whilst 17 sera gave discordant results; these 17 were also tested by serum fractionation. Problems were encountered with all assay systems used. It is therefore recommended that the results of all tests for rubella-specific IgM should be interpreted with caution.
D000328:Adult; D000910:Antibodies, Heterophile; D000914:Antibodies, Viral / Q000032:analysis*; D003429:Cross Reactions; D005189:False Positive Reactions; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D007223:Infant; D007231:Infant, Newborn; D007244:Infectious Mononucleosis / Q000276:immunology; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012217:Rheumatoid Factor; D012409:Rubella / Q000175:diagnosis* / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*
D000910:Antibodies, Heterophile; D000914:Antibodies, Viral; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D012217:Rheumatoid Factor