title: The nucleotide recognized by the Escherichia coli A restriction and modification enzyme.
abstract: The nucleotide recognition sequence for the restriction-modification enzyme of Escherichia coli A (EcoA) has been determined to be GAG-7N-GTCA. This sequence is fairly similar, but distinctly different from the two other type I restriction enzyme recognition sites known for E. coli B and E. coli K12, respectively. N6-adenosine methylation has been observed at nucleotide positions 2 and 12 within that sequence after modification by EcoA. As a reference point for mapping the single EcoA site in lambda, the position of lambda point mutation Oam29 has been determined also.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015253:Deoxyribonucleases, Type I Site-Specific; D004926:Escherichia coli / Q000201:enzymology*; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D004262:DNA Restriction Enzymes; C040446:endodeoxyribonuclease EcoAI; D015253:Deoxyribonucleases, Type I Site-Specific