Differential effects of some transition metal cations on the binding of beta-carboline-3-carboxylate and diazepam.
The interaction of benzodiazepines and beta-carbolines with metal cations was investigated. Among numerous transition metal cations, only three, CO2+, Ni2+ and Zn2+, specifically inhibited the binding of [3H]beta-carboline-3-carboxylate ethyl ester (beta-CCE). The effects of these cations on [3H]beta-CCE binding were exactly opposite to those on [3H]diazepam binding. The effects of these cations was not dependent on lipid peroxidation. The differential effect of these cations may reflect a general difference in the way agonists and antagonists bind to the benzodiazepine receptor.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002243:Carbolines / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D003975:Diazepam / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008670:Metals / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D011963:Receptors, GABA-A; D013570:Synaptic Membranes / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D002243:Carbolines; D007211:Indoles; D008670:Metals; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D003035:Cobalt; C024915:beta-carboline-3-carboxylic acid ethyl ester; D009532:Nickel; D015032:Zinc; D003975:Diazepam