The effects of ethylene chlorohydrin on fatty acid synthesis.
Male chicks weighing 700 to 900 g. received an acute or eight doses IG of 60 or 40 mg/kg ethylene chlorohydrin (ECH) respectively and were sacrificed eighteen hours after the last dose. Mitochondrial elongation of fatty acids was decreased significantly while fatty acid synthetase activity was not significantly affected by ECH treatment. Cytochrome c oxidase activity in fresh whole liver homogenate was significantly higher in chicks subjected to acute exposure with ECH when compared to the controls. Upon freezing and thawing of homogenates, cytochrome c oxidase activity increased significantly in the control group but was unchanged in the ECH group which suggests that the mitochondrial membrane integrity is compromised by the ECH treatment. Serum and liver triglyceride levels were significantly elevated in both the single and multiple ECH dose groups. Liver to body weight ratios were significantly higher in both treatment groups when compared to their controls. Histological examination of the liver of ECH-treated chicks showed cytoplasmic clearing of the cells but no vacuolization or centrilobular necrosis. Serum isocitrate dehydrogenase levels were significantly higher in the multiple treatment ECH group than in the control group.
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002645:Chickens; D002728:Chlorohydrins / Q000633:toxicity*; D003576:Electron Transport Complex IV / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005023:Ethylene Chlorohydrin / Q000494:pharmacology / Q000633:toxicity*; D005227:Fatty Acids / Q000096:biosynthesis*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects
D002728:Chlorohydrins; D005227:Fatty Acids; D000431:Ethanol; D005023:Ethylene Chlorohydrin; D002251:Carbon Tetrachloride; D007521:Isocitrate Dehydrogenase; D003576:Electron Transport Complex IV