Glycerol transport and phosphorylation by rat hepatocytes.
The entry of glycerol into isolated rat hepatocytes appears to be catalyzed by a specific carrier. At a physiological concentration of 0.1 mM, glycerol utilization is rate limited by the permeation step. Intracellular glycerol is trapped by an excess of glycerol kinase, which has a higher apparent affinity for the substrate than that of the membrane carrier. The entry of glycerol into the hepatocytes is highly sensitive to inhibition by monoacetin and cytochalasin B, but not by DL-1,2-propanediol, erythritol, D-glucose, D-galactose, D-mannose, or D-fructose.
D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D010770:Phosphotransferases; D005991:Glycerol Kinase; D005990:Glycerol