Phosphorylation of glycogen synthase in isolated rabbit hepatocytes.
Specific antibodies were used to purify glycogen synthase from isolated rabbit hepatocytes that had been incubated in a medium containing [32P]phosphate. The enzyme gave rise to two main 32P-labeled CNBr fragments of electrophoretic mobilities similar to those obtained after phosphorylation of the enzyme by individual protein kinases in vitro.
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D003488:Cyanogen Bromide; D006006:Glycogen Synthase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D010748:Phosphopeptides / Q000032:analysis; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits
D010446:Peptide Fragments; D010748:Phosphopeptides; D010761:Phosphorus Radioisotopes; D006006:Glycogen Synthase; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D003488:Cyanogen Bromide