title: The possible role of the superoxide ion in the induction of heat-shock and specific proteins in aerobic Drosophila cells during return to normoxia after a period of anaerobiosis.
abstract: In vitro cultured Drosophila melanogaster cells were shown to be aerobic and several kinetic parameters of their respiration were measured. This allowed us to define experimental conditions for a transient period of anaerobiosis followed by a reexposure to normal oxygenation. This treatment, applied without any change of temperature, induced not only the heat-shock proteins, but also a new specific peptide of 27 000 daltons and a twofold increase of the maximal rate of O2 uptake. This evokes a common molecular mechanism activated either by heat or by O2, which could involve the increase of the products of oxygen reduction such as the superoxide ion.
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D002478:Cells, Cultured; D003486:Cyanides / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000378:metabolism*; D006360:Heat-Shock Proteins; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology*; D010101:Oxygen Consumption; D014176:Protein Biosynthesis; D013481:Superoxides / Q000494:pharmacology*
chemical_list: D003486:Cyanides; D006360:Heat-Shock Proteins; D013481:Superoxides; D010100:Oxygen