title: Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae.
abstract: A new mutation has been described which confers resistance to catabolite repression in Saccharomyces cerevisiae. The mutant allele, termed grr-1 for glucose repression-resistant, is characterized by insensitivity to glucose repression for the cytoplasmic enzymes invertase, maltase, and galactokinase, as well as the mitochondrial enzyme cytochrome c oxidase. Hexokinase levels in grr-1 mutants are approximately 3-fold higher than the corresponding activity of the parental strain. Although the grr-1 allele is expressed phenotypically similarly to the hex-1 (hxk-2) and hex-2 mutations described by Entian et al. (1977) and Zimmermann and Scheel (1977) respectively, we have shown genetically and physiologically that grr-1 represents a new class of mutation.
mesh_terms: D000483:Alleles; D003433:Crosses, Genetic; D003847:Deoxyglucose / Q000633:toxicity; D003576:Electron Transport Complex IV / Q000378:metabolism; D004794:Enzyme Repression; D005690:Galactose / Q000378:metabolism; D005799:Genes, Dominant; D005800:Genes, Fungal; D005808:Genes, Recessive; D005838:Genotype; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D013045:Species Specificity
chemical_list: D003847:Deoxyglucose; D003576:Electron Transport Complex IV; D006593:Hexokinase; D005947:Glucose; D005690:Galactose