Leukotriene B4: biological activities and the cytoskeleton.
The aggregation and chemokinesis of either rat or human polymorphonuclear leucocytes (PMNs) induced by leukotriene B4 isomer III (LTB4) are inhibited significantly by colchicine (10(-6) - 10(-3)M) and vinblastine (10(-7) - 10(-4)M). Random migration of the leucocytes is inhibited by colchicine 10(-3)M and vinblastine 10(-4)M. Cytochalasin B (4 X 10(-7) - 4 X 10(-6)M) caused the aggregation of rat PMNs but inhibited their random migration. The aggregation of the PMNs induced by LTB4 was enhanced by cytochalasin B but the chemokinesis was inhibited. It is suggested that both microtubules and microfilaments may be involved in the aggregatory and chemokinetic responses of PMNs to LTB4.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000494:pharmacology*; D008870:Microtubules / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014747:Vinblastine / Q000494:pharmacology
D007975:Leukotriene B4; D003571:Cytochalasin B; D014747:Vinblastine; D003078:Colchicine