title: Two site-specific endonucleases BinSI and BinSII from Bifidobacterium infantis.
abstract: Two site-specific endonucleases, BinSI and BinSII, were isolated from Bifidobacterium infantis S76e. BinSI was found to be an isoschizomer of EcoRII, while BinSII was shown to have the same sequence and cutting specificity as BbeI, 5'-GGCGC decreases C-3'. Both BinSII- and BbeI-generated DNA fragments could be ligated with HaeII-generated DNA fragments.
mesh_terms: D001483:Base Sequence; D001644:Bifidobacterium / Q000201:enzymology*; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C046719:GGCGCC-specific type II deoxyribonucleases