title: Inhibition of erythrocyte ATPase activity by aclacinomycin and reverse effects of ascorbate on ATPase activity.
abstract: We studied the effect of aclacinomycin on human erythrocyte membrane enzymes. Aclacinomycin inhibited ATPase, including Na-K-dependent ATPase, ouabain insensitive ATPase and Ca-ATPase. However acetylcholinesterase was not inhibited by aclacinomycin. The ATPase activities were not inhibited by aclacinomycin if ascorbate was added to the incubation mixture. However other reducing agents, alpha-tocopherol, superoxide dismutase and catalase had no effect on ATPase activity. Ascorbate may protect membrane proteins and lipids from peroxidate damage.
mesh_terms: D015250:Aclarubicin / Q000031:analogs & derivatives*; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D001205:Ascorbic Acid / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D009279:Naphthacenes / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D009279:Naphthacenes; C011157:aclacinomycins; D010042:Ouabain; D015250:Aclarubicin; D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D001205:Ascorbic Acid