Fructose 1,6-bisphosphatase in the diagnosis of chronic hepatitis. I. Activity measurements of fructose 1,6-bisphosphatase in human serum.
In this communication, we propose a method for the determination in human serum of fructose 1,6-bisphosphatase based on parallel measurements of enzyme activities in presence of 1-p-bromotetramisole oxalate and adenosine 5'-monophosphate. The employment of these specific inhibitors renders the discrimination between specific and non-specific activities feasible. A regression analysis identifies fructose 1,6-bisphosphatase (EC 3.1.3.11) as the origin of the specific and alkaline phosphatase (EC 3.1.3.1) as the source of the non-specific fructose 1,6-bisphosphate dephosphorylating activities. This procedure lends itself to the diagnosis using serum samples of 'piecemeal' necrosis in liver disease.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000097:blood; D002908:Chronic Disease; D004796:Clinical Enzyme Tests; D003937:Diagnosis, Differential; D005260:Female; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D006505:Hepatitis / Q000175:diagnosis*; D006801:Humans; D008297:Male; D013773:Tetramisole / Q000031:analogs & derivatives / Q000494:pharmacology
D000249:Adenosine Monophosphate; C006226:4-bromotetramisole; D013773:Tetramisole; D000469:Alkaline Phosphatase; D006597:Fructose-Bisphosphatase