[Study of thyroid autoimmunity in man. Contribution of cell cultures to the study of antibodies stimulating the thyroid gland in Basedow's disease].
The value of thyroid cell cultures in vitro has been demonstrated in auto-immune thyroiditis in animals. We have used the same method in man to investigate for serum thyroid-stimulating antibodies (TSAb) by measuring cyclic AMP production in thyroid cell cultures incubated with immunoglobulins from patients with Graves' disease. The specificity of the reaction is strictly directed either against TSH or against immunoglobulins from patients with Graves' disease; 92% of the sera of our untreated Graves' disease patients were positive. The value of this technique compared to the other methods used for detecting TSAb is discussed.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000032:analysis*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006111:Graves Disease / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D007223:Infant; D008297:Male; D008875:Middle Aged; D013961:Thyroid Gland / Q000276:immunology*; D013972:Thyrotropin / Q000494:pharmacology; D013997:Time Factors
D001323:Autoantibodies; D007074:Immunoglobulin G; D013972:Thyrotropin; D000242:Cyclic AMP
Exploration chez l'homme de l'auto-immunité thyroïdienne. Apport des cultures cellulaires à l'étude des anticorps stimulant la thyroïde dans la maladie de Basedow.