Immunogenicity of equine herpesvirus type 1 (EHV1) and equine rhinovirus type 1 (ERhV1) following inactivation by betapropiolactone (BPL) and ultraviolet (UV) light.
Some kinetic data on the inactivation of equine herpesvirus type 1 (EHV1) and equine rhinovirus type 1 (ERhV1) by betapropiolactone (BPL) and ultraviolet (UV) irradiation are reported. 0.25% BPL at 37 degrees C for 1 h reduced the titre of EHV1 by greater than 10(3 . 4) and of ERhV1 by greater than 10(4 . 1) TCID50/ml. UV irradiation (334 microW/cm2) produced similar reductions in titre after 2 min. These data were used as a basis for inactivating EHV1 and ERhV1 by the combined action of BPL and UV irradiation. Viruses were exposed to 0.1% BPL for 1 h at 4 degrees C with constant stirring, followed by UV irradiation for 2 min, followed by incubation for 3 h at 37 degrees C. Inactivated EHV1 elicited secondary immune responses only in horses whereas ERhV1 produced primary immune responses in mice (including athymic nu/nu mice), rabbits and probably in horses.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D006564:Herpesviridae / Q000276:immunology*; D004861:Herpesvirus 1, Equid / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D006736:Horses / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D051379:Mice; D008819:Mice, Nude / Q000276:immunology*; D010849:Picornaviridae / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D011420:Propiolactone / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology*; D012229:Rhinovirus / Q000276:immunology*; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D011420:Propiolactone