title: [Incorporation of a DNA replica of the genome of the tick-borne encephalitis virus into cellular DNA].
abstract: DNA from mouse cells chronically infected with tick-borne encephalitis (TBE) virus was treated with restrictases, the resulting fragments were fractionated by size by gel electrophoresis, denaturated, and transferred from gel on nitro-cellulose filters. The fragments containing virus-specific sequences were detected by hybridization with 32P-DNA replicas of TBE genome RNA synthesized using reverse transcriptase. The presence of virus-specific sequences in DNA fragments from chronically infected cells proves the possibility of integration of DNA-replicas of TBE virus genome and genome of chronically infected cells.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics*; D004669:Encephalitis Viruses, Tick-Borne / Q000187:drug effects / Q000235:genetics*; D004675:Encephalitis, Tick-Borne / Q000235:genetics; D005814:Genes, Viral / Q000187:drug effects; D007739:L Cells / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D012367:RNA, Viral / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes
vernacular_title: Vstraivanie DNK-kopii genoma virusa kleshchevogo éntsefalita v kletochnuiu DNK.