Inhibition of ADP-ribosyltransferase activity of cholera toxin by MDL 12330A and chlorpromazine.
ADP-ribosylation by cholera toxin of the guanine nucleotide binding regulatory protein (Gs) of rat liver membrane adenylate cyclase was inhibited by 0.1-1 mM MDL 12330A or 0.1-1 mM chlorpromazine. Basal as well as cholera toxin activated adenylate cyclase activity in liver membranes was also inhibited by the two drugs. NAD glycohydrolase activity and self-ADP-ribosylation of cholera toxin were also inhibited by MDL 12330A and chlorpromazine. These effects of MDL 12330A and chlorpromazine may be related to their effects on cholera toxin-induced fluid secretion in vivo.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002746:Chlorpromazine / Q000494:pharmacology*; D002772:Cholera Toxin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology; D006801:Humans; D007097:Imines / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D009699:N-Glycosyl Hydrolases / Q000037:antagonists & inhibitors; D009244:NAD+ Nucleosidase; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D011065:Poly(ADP-ribose) Polymerases; D051381:Rats
D000067956:Adenylyl Cyclase Inhibitors; D007097:Imines; D000246:Adenosine Diphosphate Ribose; C014925:RMI 12330A; D002772:Cholera Toxin; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase; D002746:Chlorpromazine