title: A physical map of the group A streptococcal pyrogenic exotoxin bacteriophage T12 genome.
abstract: A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases Sal/I, PstI, and EcoRI. The map is circularly permuted with a total length of 36.0 kb. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW, were observed. This observation, together with mapping data, suggests that DNA packaging is initiated at a precise site on a concatemeric precursor and proceeds for a limited number of rounds.
mesh_terms: D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005098:Exotoxins; D005814:Genes, Viral; D008565:Membrane Proteins; D013297:Streptococcus pyogenes / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004279:DNA, Viral; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; D004262:DNA Restriction Enzymes