[Mechanism of DNA secondary postradiation degradation in the thymocytes of irradiated rats. 1. The substrate specificity of alkaline endonucleases].
The spectrophotometric and viscosimetric methods were used to study the substrate specificity and optimum pH of alkaline endonucleases in irradiated and nonirradiated cell homogenates of rat thymocytes. It was shown that activation of alkaline endonucleases in cell homogenates of thymocytes, as determined by the spectrophotometric method, was connected with the increase in DNAase I activity. The viscosimetric method fixed the activation of several alkaline endonucleases including DNAase I.
D000818:Animals; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000528:radiation effects; D004720:Endonucleases / Q000528:radiation effects*; D004789:Enzyme Activation / Q000528:radiation effects; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D013379:Substrate Specificity / Q000528:radiation effects; D013950:Thymus Gland / Q000201:enzymology / Q000528:radiation effects*; D013997:Time Factors
D004277:DNA, Single-Stranded; D004247:DNA; D004720:Endonucleases
Mekhanizm vtorichnoĭ postradiatsionnoĭ degradatsii DNK v timo-tsitakh obluchennykh krys. Soobshchenie 1. Substratnaia spetsifichnost' shchelochnykh éndonukleaz.