Proteinases and sialyltransferase in human breast tumors.
Proteolytic and sialyltransferase activities were determined in extracts of 65 human primary breast tumors, 6 lymph node metastases, 6 fibroadenomas and 27 normal tissues. Using proteins and synthetic selective substrates, we observed the presence of collagen-peptidases, plasminogen activator, cathepsin-B and cathepsin-D-like enzymes, and sialyltransferase. No active or trypsin-activatable type-IV collagenase activity was detected. Although individual variations between tumors were large, proteinase and sialyltransferase contents were significantly elevated in malignant breast tissues. Enzyme activities were found to be related to the epithelial volume of the tumor. No significant correlation was found between the proteinase or sialyltransferase activities and the degree of differentiation of the tumor cells, or the degree to which tumors had metastasized to regional lymph nodes. Since large variations of enzyme levels apparently reflect the heterogeneity of epithelial cell densities in tumor samples, proteolytic or sialyltransferase activities cannot therefore be used as a measure of quantitative evaluation of invasive properties in breast cancer.
D000230:Adenocarcinoma / Q000201:enzymology; D000232:Adenofibroma / Q000201:enzymology; D001940:Breast / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000201:enzymology; D002401:Cathepsin B; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism; D002452:Cell Count; D010450:Endopeptidases / Q000378:metabolism*; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D003012:Microbial Collagenase / Q000378:metabolism; D010960:Plasminogen Activators / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D014166:Transferases; D012799:Sialyltransferases; D002403:Cathepsins; D010450:Endopeptidases; D010960:Plasminogen Activators; D002401:Cathepsin B; D002402:Cathepsin D; D003012:Microbial Collagenase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase