title: Presynaptic involvement in frequency facilitation in the hippocampal slice.
abstract: Orthodromic stimulation with frequencies from 1 to 30 Hz causes facilitation of extracellularly recorded population spikes and a decrease of extracellular Ca2+ concentration ( [Ca2+]o ) monitored with ion selective microelectrodes at the cell body layer of area CA1 of the hippocampal slice. Reducing the Ca2+ content of the perfusion medium impairs synaptic transmission. However, even under these conditions, weak stimulation evokes a significant decrease of [Ca2+]o, which is ascribed to a presynaptic Ca2+ entry. Stimulation with high intensities and/or frequencies initially induces an accelerated decrease in [Ca2+]o followed by the re-establishment of synaptic transmission, indicating a contribution of the presynaptic site to frequency facilitation.
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D046508:Culture Techniques; D004558:Electric Stimulation; D006624:Hippocampus / Q000502:physiology*; D008564:Membrane Potentials; D051381:Rats; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: D002118:Calcium