title: [Comparison of methods for the large-scale culture of cells and viruses].
abstract: The paper presents the results of experiments on propagation of primary, secondary, and continuous diploid and heteroploid human and animal cells in 2 different systems for large-scale propagation: in perfusion tank with Rashig rings and in tanks with microcarriers. Both methods of large-scale cultivation produce higher cell yields than the traditional cultivation methods. A yield of tick-borne encephalitis virus per 1 cell in the perfusion cultivator was 12 times as high as in roller cultures. Poliomyelitis virus titres were practically equal with both methods of large-scale cultivation and with the conventional method (7.73 lg PFU/ml in GMKC on microcarriers). The method of cultivation on microcarriers is more acceptable and advantageous as it gives higher yields of cells necessary for growth of poliomyelitis virus.
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000382:microbiology*; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D003584:Cytological Techniques; D004669:Encephalitis Viruses, Tick-Borne / Q000254:growth & development; D007668:Kidney; D017955:Poliovirus / Q000254:growth & development; D013997:Time Factors; D014765:Viral Vaccines / Q000302:isolation & purification; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
chemical_list: D014765:Viral Vaccines
vernacular_title: Sravnenie metodov krupnomasshtabnogo kul'tivirovaniia kletok i virsuov.