Growth inhibition of human breast cancer cells induced by calcitonin.
The human breast cancer cell line (T47D) has specific, high affinity calcitonin receptors and calcitonin-responsive adenylate cyclase. Human, salmon and [Asu1,7]eel calcitonin inhibited cell growth in a dose-related manner with almost equipotency. Analogues of human calcitonin demonstrated slight cell growth inhibition. We found extreme growth inhibition with daily treatment with dibutyryl cyclic AMP (10(-4) M). In contrast to calcitonin 1,25-(OH)2D3 had a biphasic effect on cell growth. Physiological doses (5 X 10(-10) M) of 1,25-(OH)2D3 stimulated growth of T47D, whereas treatment by supraphysiological amounts (2.5 X 10(-7) M) caused significant inhibition of growth. Calcitonin and 1,25-(OH)2D3 appeared to have additive effects.
D000818:Animals; D001943:Breast Neoplasms / Q000503:physiopathology*; D003994:Bucladesine / Q000494:pharmacology; D002116:Calcitonin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004273:DNA, Neoplasm / Q000378:metabolism; D004524:Eels; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D012474:Salmon; D013045:Species Specificity; D013329:Structure-Activity Relationship
D004273:DNA, Neoplasm; D004958:Estradiol; D003994:Bucladesine; D002116:Calcitonin