The susceptibility of the mesangial matrix of isolated cell-free glomeruli to limited proteolysis.
Selective lysis of mesangial matrix can be achieved by limited proteolysis of isolated cell-free glomeruli. Such treated glomeruli present unfolded and confluent capillary loops forming irregularly shaped, saccular lobular structures, comparable to those described for chemical mesangiolysis. It was observed that the parietal capsules of the glomeruli were also susceptible to limited proteolysis by comparison with the resistance of the basement membranes of the peripheral capillary loops.
D000818:Animals; D010450:Endopeptidases / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000187:drug effects*; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000494:pharmacology; D008854:Microscopy, Electron; D010206:Papain / Q000494:pharmacology; D010434:Pepsin A / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
D010450:Endopeptidases; D014357:Trypsin; D010206:Papain; D010434:Pepsin A; D011402:Pronase; D003012:Microbial Collagenase