title: Penicillinase as a marker in enzyme-linked immunosorbent assays for steroid hormones.
abstract: Using penicillinase (E.C. 3.5.2.6) as marker and microtitre ELISA plates for immunoadsorption, sensitive and specific assays for two steroid hormones viz. testosterone in plasma and estrone-3-glucuronide in urine were developed. The entire assay can be performed in 4 h and requires simple laboratory equipment such as an incubator and a colorimeter. The enzyme labelled steroids have a shelf life of more than 3 years.
mesh_terms: D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004966:Estrogens, Conjugated (USP) / Q000097:blood*; D004970:Estrone / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D006801:Humans; D007106:Immune Sera; D008832:Microchemistry; D010405:Penicillinase / Q000032:analysis*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D004966:Estrogens, Conjugated (USP); D007106:Immune Sera; C015856:estrone-3-glucuronide; D004970:Estrone; D013739:Testosterone; C039006:testosterone-3-oxime; D010405:Penicillinase; D001618:beta-Lactamases