title: Isolation of a tumor cell laminin receptor.
abstract: BL6 murine melanoma cells contain approximately 110,000 cell surface binding sites for the basement membrane glycoprotein laminin. Treatment of isolated melanoma cell plasma membranes with detergent yields a single class of laminin receptor. The receptor was purified 900 fold by laminin affinity chromatography. The isolated receptor has a Mr of 67,000 and binds laminin with high affinity: kd = 2 nm. The binding affinity of the isolated receptor was similar to that of the plasma membranes or the whole cells. Such a laminin receptor, isolated here for the first time, could facilitate the interaction of metastasizing tumor cells with the basement membrane.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003902:Detergents; D006023:Glycoproteins / Q000378:metabolism*; D007797:Laminin; D008545:Melanoma / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D017474:Receptors, Laminin; D013499:Surface Properties
chemical_list: D003902:Detergents; D006023:Glycoproteins; D007797:Laminin; D011956:Receptors, Cell Surface; D017474:Receptors, Laminin