title: Site-directed point mutation in the src gene oF rous sarcoma virus results in an inactive src gene product.
abstract: Site-directed mutagenesis techniques were used to construct defined point mutations within the src gene of the Prague A strain of Rous sarcoma virus. Bisulfite mutagenesis at a Bg/I restriction site in the src gene yielded three mutations which contained the same single base change, a guanine-to-adenine transition. The resulting genomes encoded an src protein containing a substitution of threonine for alanine at amino acid position 433. Transfection of chicken cells with mutagenized DNA did not result in cellular transformation even though the cells produced a pp60src. Immune complexes containing mutant pp60src did not phosphorylate immunoglobulin G heavy chain or casein.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D011494:Protein Kinases / Q000235:genetics* / Q000502:physiology; D011505:Protein-Tyrosine Kinases; D013447:Sulfites / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D013447:Sulfites; D014764:Viral Proteins; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src); C009279:sodium bisulfite