title: The oxidative burst and related phenomena in mouse macrophages elicited by different sterile inflammatory stimuli.
abstract: Mouse peritoneal macrophages (MPM) elicited by paraffin oil (PO) or thioglycollate (TG) were compared in their capacity to generate oxidative burst (OB) products such as superoxide ion (O2-) and hydrogen peroxide (H2O2) and lyse target erythrocytes. Strong OB stimulants such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA), opsonized zymosan; and wheat-germ agglutinin (WGA) were found to stimulate O2 and H2O2 production by both PO- and TG-elicited MPM. PO-elicited MPM exhibited a vigorous OB response compared to TG-elicited MPM and showed a higher degree of self iodination in the presence of 125I. Macrophage-mediated cytolysis of 51Cr-labeled red blood cells, following OB stimulation, was revealed by PO-elicited MPM triggered by TPA, opsonized zymosan, WGA, and Concanavalin A. TG-elicited MPM failed to respond to TPA and opsonized zymosan. In the presence of horseradish peroxidase, both PO- and TG-elicited MPM exhibited augmented cytocidal activities upon stimulation with TPA, whereas catalase abrogated the capacity of TPA-stimulated MPM to lyse red blood cells, which may suggest the involvement of H2O2 in the lytic process.
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology; D007457:Iodine Radioisotopes / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010100:Oxygen / Q000378:metabolism*; D013481:Superoxides / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014909:Wheat Germ Agglutinins; D015054:Zymosan / Q000494:pharmacology
chemical_list: D007457:Iodine Radioisotopes; D037102:Lectins; D014909:Wheat Germ Agglutinins; D013481:Superoxides; D015054:Zymosan; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen