Pepsinogen secretion from dispersed glands from rabbit stomach.
In dispersed glands from rabbit stomach, pepsinogen secretion was stimulated by carbamylcholine, the C-terminal octapeptide of cholecystokinin (CCK-8), and structurally related peptides physalaemin and A23187 but not by bombesin or histamine. Reducing the incubation temperature from 37 degrees to 4 degrees C or adding carbonyl cyanide m-chlorophenylhydrazone reduced basal as well as stimulated pepsinogen secretion. Dibutyryl cGMP inhibited the stimulation of pepsinogen secretion caused by cholecystokinin but not that caused by carbamylcholine; atropine inhibited the stimulation of pepsinogen secretion caused by carbamylcholine but not that caused by cholecystokinin. Each cholecystokinin-related peptide had the same efficacy for stimulating pepsinogen secretion. In terms of the concentration that caused half-maximal stimulation of pepsinogen secretion, the relative potencies of cholecystokinin-related peptides were caerulein greater than CCK-8 greater than [desSO3]CCK-8 greater than gastrin. Removing extracellular calcium did not alter basal or cholecystokinin-stimulated pepsinogen secretion. These results illustrate that dispersed glands from rabbit stomach constitute a suitable preparation for examining the actions of various agents on pepsinogen secretion in vitro.
D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002766:Cholecystokinin / Q000494:pharmacology; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D005753:Gastric Mucosa / Q000201:enzymology*; D006632:Histamine / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D010435:Pepsinogens / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D010803:Physalaemin / Q000494:pharmacology; D011817:Rabbits; D012627:Sebaceous Glands / Q000201:enzymology*; D012844:Sincalide
D010435:Pepsinogens; D010446:Peptide Fragments; D010803:Physalaemin; D003995:Dibutyryl Cyclic GMP; D000001:Calcimycin; D006632:Histamine; D002217:Carbachol; D002766:Cholecystokinin; D012844:Sincalide; D001839:Bombesin