Quantitation of ketoconazole in biological fluids using high-performance liquid chromatography.
A rapid, specific procedure is described for the quantitation of ketoconazole in biological fluids using high-performance liquid chromatography (HPLC). The procedure involves sample preparation using a reverse-phase C-18 cartridge prior to chromatography and quantitation using peak height ratios (UV absorbance detection, 231 nm) of ketoconazole to the internal standard, phenothiazine. A sensitivity of 0.2 micrograms/ml was achieved using a 0.5-ml sample. The mean recovery was 86.2%, and overall coefficient of variation of the procedure was 7.1%. This procedure has been used to determine ketoconazole levels in human serum, plasma, CSF, and synovial fluid. A comparison with a microbiological assay is presented, and adaptability of this procedure to quantitation by fluorescence to increase the sensitivity fivefold is discussed.
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007654:Ketoconazole / Q000032:analysis* / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D007654:Ketoconazole