title: The product of the avian erythroblastosis virus erbB locus is a glycoprotein.
abstract: Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. A locus, v-erbB, within the viral genome has been implicated in AEV-mediated oncogenesis. We report here the detection and partial characterization of the protein product of the v-erbB oncogene in AEV-transformed cells. We obtained the antisera necessary for our analysis by expressing a portion of the molecularly cloned v-erbB locus in Escherichia coli and immunizing rabbits with the resulting bacterial erbB polypeptide. Antisera directed against the bacterial polypeptide reacted with v-erbB proteins obtained from virus-infected avian cells. By three criteria--tunicamycin inhibition, lectin binding and metabolic labeling with radioactive sugar precursors--the product of the v-erbB gene appears to be a glycoprotein.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D004900:Erythroblasts / Q000382:microbiology; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008970:Molecular Weight; D009857:Oncogenes; D011499:Protein Processing, Post-Translational; D014415:Tunicamycin / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins; D014415:Tunicamycin