Restriction endonuclease cleavage analysis of adenovirus type 8: two new subtypes from patients with epidemic keratoconjunctivitis in Sapporo, Japan.
We studied the restriction endonuclease cleavage patterns of DNAs of adenovirus type 8 (Ad 8) isolated from epidemic keratoconjunctivitis cases. DNAs of 25 Ad 8 isolates collected during the period from 1975 to 1981 in Sapporo were subjected to enzymatic cleavage with PstI, BamHl , HindIII and SalI. On the basis of the cleavage patterns, the isolates were divided into two subtypes, A and B, both different from Trim strain used as the Ad 8 prototype strain. Subtype A was prevalent in the period from 1975 to 1978, while subtype B in the period from 1976 to 1981.
D000257:Adenoviridae Infections / Q000382:microbiology*; D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000382:microbiology*; D000260:Adenoviruses, Human / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007564:Japan; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000382:microbiology*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes