Localization of low-melting regions in phage T7 DNA.
Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8+/-0.3;46.3+/-0.5; 98.4+/-0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.Images
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D004720:Endonucleases; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013604:T-Phages / Q000235:genetics*
D004279:DNA, Viral; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases