Specific proteolytic fragmentation of p60v-src in transformed cell lysates.
Work involving the transforming protein, p60v-src, of Rous sarcoma virus has resulted in the extensive characterization of its protein structure and associated phosphotransferase activity. However, in many investigations proteolytic fragments (principally p52v-src) of the src protein are actually studied. Here, we emphasize potential problems in the interpretation of experimental results in which the proteolytic fragmentation of p60v-src may be involved and offer several means for the complete prevention of this p60v-src degradation.
D000818:Animals; D003411:Arvicolinae; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002021:Buffers; D002458:Cell Fractionation; D002460:Cell Line; D002472:Cell Transformation, Viral; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D015688:Oncogene Protein pp60(v-src); D010770:Phosphotransferases; D014764:Viral Proteins / Q000302:isolation & purification*
D002021:Buffers; D014764:Viral Proteins; D010770:Phosphotransferases; D015688:Oncogene Protein pp60(v-src)