Affinity labelling of the tetrodotoxin-binding component of the Na+ channel.
Tetrodotoxin-binding sites were covalently labelled with a highly tritiated derivative of tetrodotoxin. Cross-linking experiments, using dissucinimidyl suberate, on partially purified tetrodotoxin-binding component from electroplax of Electrophorus electricus, revealed covalent labelling of a single polypeptide chain of MW 270,000.
D000345:Affinity Labels / Q000494:pharmacology*; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004557:Electric Organ / Q000378:metabolism*; D004593:Electrophorus; D007473:Ion Channels / Q000378:metabolism*; D008970:Molecular Weight; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels; D013779:Tetrodotoxin / Q000378:metabolism*
D000345:Affinity Labels; D002352:Carrier Proteins; D007473:Ion Channels; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D013779:Tetrodotoxin; D012964:Sodium