Antigenic and structural studies on the transforming proteins of Rous sarcoma virus and Yamaguchi 73 avian sarcoma virus.
A widely cross reactive antiserum raised against denatured pp60v-src, the transforming protein encoded by Rous sarcoma virus, was used to test antigenic relationships with the transforming gene products encoded by other avian sarcoma viruses. The results showed that P90gag-yes, the transforming protein of a representative of Class III avian sarcoma viruses, is antigenically related to pp60v-src. Tryptic phosphopeptide analysis of P90gag-yes revealed two phosphotyrosine-containing peptides and one phosphoserine-containing peptide. One of the phosphotyrosine-containing peptides comigrated with the phosphotyrosine-containing tryptic peptide from pp60v-src.
D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D005814:Genes, Viral; D010748:Phosphopeptides / Q000032:analysis / Q000276:immunology; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000956:Antigens, Viral; D010748:Phosphopeptides; D014764:Viral Proteins