title: Autoradiographic demonstration of peripheral adenosine binding sites using [3H]NECA.
abstract: Autoradiographic localization of 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) binding sites revealed a heterogeneous labeling of guinea-pig intestine with heavy labeling over the enteric ganglia and in clusters over the mucosa; a low level of label was homogeneously distributed over the muscularis externa. Under the conditions employed, no binding sites were revealed using [3H]-N6-cyclohexyladenosine ([3H]CHA), although both [3H]CHA and [3H]NECA binding sites were localized over comparable areas of rat brain. The relationship of the [3H]NECA binding sites to extracellular adenosine receptors is discussed.
mesh_terms: D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002432:Cecum / Q000378:metabolism*; D006168:Guinea Pigs; D007082:Ileum / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D009197:Myenteric Plexus / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011983:Receptors, Purinergic
chemical_list: D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D015762:2-Chloroadenosine; D019830:Adenosine-5'-(N-ethylcarboxamide); C027513:N(6)-cyclohexyladenosine; D000241:Adenosine