title: Chondrogenesis of chick limb mesenchyme in vitro. Effects of prostaglandins on cyclic AMP.
abstract: The effects of prostaglandin E2 (PGE2) on cyclic AMP (cAMP) levels of chick limb bud cells during various stages of chondrogenesis were studied utilizing high density, micro-mass, cell culture. Concentrations of PGE2 in cell cultures at these same stages were measured by radio-immunoassay. Both basal levels of cAMP, as well as PGE2-stimulated changes in cAMP, increased maximally during the first 3 days of culture; this increase was associated with the formation of cell aggregates. Concentrations of PGE2 were also highest during this period. By 6 days of culture, nodules, containing cartilage matrix components, predominated. Both basal levels of cAMP and PGE2-stimulated cAMP levels were significantly decreased at this stage, relative to cultures at day 3. Concentrations of PGE2 fell dramatically in the 6-day cultures containing differentiated cartilage. These results support a regulatory role for both PGE2 and cAMP in the early events associated with chondrogenesis.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone; D005121:Extremities / Q000196:embryology; D007700:Kinetics; D011458:Prostaglandins E / Q000502:physiology*
chemical_list: D011458:Prostaglandins E; D000242:Cyclic AMP; D015232:Dinoprostone