5'-Nucleotidase activity in subjects with abnormal lymphocyte function.
5'-Nucleotidase (5'N) activity was determined in lymphocytes from patients with immunodeficiency diseases and in T-lymphocyte subpopulations from normal subjects. Cells from two patients with DiGeorge syndrome, with normal levels of B cells, and one patient with partial DiGeorge syndrome were found to have diminished 5'N activity. Utilizing monoclonal antibodies to deplete T-lymphocyte subpopulations, we found similar levels of 5'N in the cells remaining after depletion of OKT4- or OKT8-positive cells when 5'N values were determined after overnight incubation. If 5'N activity was determined on the day of the fractionation, however, OKT4-depleted cells had approximately threefold greater enzyme activity. These studies indicate that 5'N activity may vary in T lymphocyte subpopulations depending upon cell culture conditions. Diminished levels of 5'N activity are seen in patients with abnormal T-lymphocyte differentiation, as well as abnormalities of B-lymphocyte differentiation.
D015720:5'-Nucleotidase; D000328:Adult; D000361:Agammaglobulinemia / Q000151:congenital / Q000201:enzymology; D000911:Antibodies, Monoclonal / Q000276:immunology; D004062:DiGeorge Syndrome / Q000201:enzymology; D005260:Female; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000201:enzymology*; D007223:Infant; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D013601:T-Lymphocytes / Q000145:classification / Q000201:enzymology
D000911:Antibodies, Monoclonal; D009708:Nucleotidases; D015720:5'-Nucleotidase