Platelet-mediated collagen and fibrin retraction: effect of prostaglandins, cyclic AMP, calcium antagonists and N-ethylmaleimide.
Collagen gel retraction (CGR) and thrombin-induced fibrin clot retraction (FCR) could be abolished by EGTA and EDTA, trifluoperazine - a calmodulin inhibitor - and by NEM. Both processes appeared insensitive to hydrocortisone (up to 100 microM), indomethacin (up to 60 microM), PGI2 and its stable analogue 6-keto-PGE1 alpha (10 microM). A slight inhibitory effect on both CGR and FCR was provoked by dibutyryl-cAMP and PGD2. PGE1 1 microM strongly inhibited CGR and FCR. Possible interference of the inhibitors with anchorage of collagen and fibrin on platelet surface and with platelet contractile system is discussed.
D001792:Blood Platelets / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003018:Clot Retraction; D003094:Collagen / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology*; D005033:Ethylmaleimide / Q000494:pharmacology*; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011453:Prostaglandins / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D011453:Prostaglandins; D005337:Fibrin; D003094:Collagen; D000242:Cyclic AMP; D005033:Ethylmaleimide