title: Biochemical mechanisms of acid secretion by gastric parietal cells.
abstract: Knowledge of the biochemical basis of gastric acid secretion has progressed dramatically in the last 25-30 years. Today, only the overall physiological consequences can be described by the carbonic anhydrase reaction; the biochemical details are much more complicated and remain incompletely resolved. The controversy between a redox, directly substrate linked, mechanism and an ATPase mechanism seems largely resolved. The marked redox changes in gastric mitochondria have been ascribed to anoxia of in vitro mucosae since isolated cells and gastric glands do not demonstrate these transitions. These findings are also in accord with stable content of NAD/NADH and NADP/NADPH in resting and secreting in vivo canine gastric mucosa. The subsequent finding that acid secretion by permeabilized gastric glands is ATP dependent even when substrate and mitochondrial metabolism is blocked by cyanide is convincing support for a proton-ATPase mechanism. Techniques for isolation and purification of mucosal membranes have yielded considerable progress in defining the enzymatic mechanism of acid secretion. Electron microscopy has demonstrated the unique system of intra-cellular membranes of the parietal cell. Several investigators have isolated these membranes by centrifugation and free-flow electrophoresis of mucosal cell membranes. In the purest preparations the membranes appear to contain only a few peptides, most of which seem to be components of the gastric ATPase. SDS polyacrylamide electrophoresis of these purified membranes has demonstrated a 100K dalton peptide that is phosphorylated in the presence of ATP. Radiation target analysis suggests that the native ATPase exists as a trimer of the 100¿K dalton peptide.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004285:Dogs; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000201:enzymology / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D006859:Hydrogen / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D010295:Parietal Cells, Gastric / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D006859:Hydrogen; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase