title: Induction of stress proteins in Sindbis virus- and vesicular stomatitis virus-infected cells.
abstract: Two proteins which are related to certain proteins induced by hyperthermia (heat shock proteins; hsp) are synthesized during lytic infection of chick embryo (CE) cells by Sindbis virus or vesicular stomatitis virus (VSV). Incubation of the infected cells at elevated temperature further increased the rate of synthesis of these proteins. The stress proteins induced by Sindbis virus had different mobilities on SDS-polyacrylamide gels compared to related stress proteins induced in mock-infected CE cells. Induction of the stress proteins in Sindbis virus- and VSV-infected CE cells was actinomycin D sensitive. Kinetic studies indicated that induction of the stress proteins is an early event during infection. The lytic virus-induced selective termination of host protein synthesis did not affect the synthesis of these proteins. Furthermore, the synthesis of these virus-induced stress proteins was resistant, relative to the synthesis of most host proteins, to alterations in the intracellular concentrations of Na+ and K+. The synthesis of a protein related to a major low-molecular-weight hsp of CE cells was not induced after Sindbis virus or VSV infection. Immunoprecipitation experiments and sedimentation analyses demonstrated that significant levels of the capsid protein (C) of Sindbis virus and nucleocapsid protein (N) of VSV are physically associated with a hsp in lysates of infected CE cells.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002213:Capsid / Q000032:analysis; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D006358:Hot Temperature; D007700:Kinetics; D008970:Molecular Weight; D012845:Sindbis Virus / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology*
chemical_list: D000596:Amino Acids; D006360:Heat-Shock Proteins; D003609:Dactinomycin; D012965:Sodium Chloride