title: Rotaviruses code for two types of glycoprotein precursors.
abstract: Rotaviruses are nonenveloped viruses that code for two glycoproteins: a structural glycoprotein (VP7) and a nonstructural glycoprotein (NS29). The precursor to VP7 (37K) was shown to contain a 1.5K cleavable signal sequence. The 37K precursor was authentically processed (signal sequence cleaved and the polypeptide "core" glycosylated) when synthesized in a cell-free system supplemented with dog pancreatic microsomes. Similar experiments were performed with the nonstructural glycoprotein precursor (20K); however, the 20K precursor contained an integral (noncleavable) signal sequence. Both precursors were inserted into membranes cotranslationally and both glycosylated products underwent posttranslational oligosaccharide processing. The results suggest a morphogenetic scheme for the simian rotavirus SA11.
mesh_terms: D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger; D012401:Rotavirus / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D006023:Glycoproteins; D011498:Protein Precursors; D012333:RNA, Messenger