Genetic identification of an endoproteinase encoded by the adenovirus genome.
The temperature-sensitive adenovirus type 2 mutant H2ts1 is defective for polypeptide processing at the non-permissive temperature. We have in the present study mapped the mutation by marker rescue and DNA sequencing techniques: the mutation is a C/T transition located at map co-ordinate 61.1. Previous sequencing studies have identified an uninterrupted translational reading frame in this part of the adenovirus genome, encoding a hypothetical 23 X 10(3) Mr polypeptide. The mutation leads to a proline/leucine substitution in the 23 X 10(3) Mr polypeptide.
D000260:Adenoviruses, Human / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D003854:Deoxyribonucleotides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D009154:Mutation; D010447:Peptide Hydrolases / Q000235:genetics*
D004279:DNA, Viral; D003854:Deoxyribonucleotides; D004262:DNA Restriction Enzymes; D010447:Peptide Hydrolases