Technical note: a new method for preparing renal tissue for microdissection.
The rat kidney has been prepared for microdissection by in vivo perfusion with hyaluronidase and collagenase and subsequent fixation with glutaraldehyde. The procedure ensures preservation of cytoplasmic detail, and the principle of fixation after maceration should provide a foundation for developing a variety of staining and histochemical studies.
D000818:Animals; D004210:Dissection / Q000379:methods; D005976:Glutaral; D006652:Histological Techniques; D006821:Hyaluronoglucosaminidase; D007668:Kidney / Q000033:anatomy & histology*; D008297:Male; D003012:Microbial Collagenase; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase; D005976:Glutaral