title: Use of integrative transformation of yeast in the cloning of mutant genes and large segments of contiguous chromosomal sequences.
mesh_terms: D001483:Base Sequence; D002875:Chromosomes / Q000502:physiology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005818:Genetic Engineering / Q000379:methods; D007202:Indicators and Reagents; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D007202:Indicators and Reagents; D004262:DNA Restriction Enzymes