Regional distribution and ionic requirement of Cl-/Ca2+-activated and Cl-/Ca2+-independent glutamate receptors in rat brain.
Recent studies have shown that Cl- and Ca2+ ions increase [3H]glutamate binding to rat forebrain synaptic plasma membranes by expressing a new class of glutamate receptors. We examined the regional distribution of these two classes of glutamate binding sites and further characterized their ionic requirements. Significant differences in both Cl-/Ca2+-independent (basal) and Cl-/Ca2+-activated receptors, as well as the ratios of these two receptor classes were observed among different areas of the CNS. Cl- and Ca2+ appeared to act synergistically, with Cl-ion an absolute requirement for Ca2+ stimulation, in expressing these additional binding sites. Ca2+ alone did not affect glutamate binding.
D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000502:physiology*; D002712:Chlorides / Q000502:physiology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000502:physiology; D017470:Receptors, Glutamate; D013116:Spinal Cord / Q000032:analysis
D000613:Aminobutyrates; D002712:Chlorides; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D004533:Egtazic Acid; D004492:Edetic Acid; C012729:2-amino-4-phosphonobutyric acid; D002118:Calcium