title: High-performance liquid chromatography of transfer RNAs. Separation of transfer RNAs from mammalian sources.
abstract: A survey of recent advances in high-performance liquid chromatography (HPLC) of tRNA is presented here. The polystyrene and reversed-phase anion exchangers are discussed for their ability to resolve tRNAs without loss of the aminoacyl-tRNA bond. The HPLC of a tRNA of choice, based on the affinity principle, is studied. Both chemical (boronate) and biological (plant lectins) affinity groups for the tRNA interaction are described. A comprehensive scheme is presented for the separation of four mammalian tRNAs. Scope of future research in this area is also discussed.
mesh_terms: D000596:Amino Acids / Q000235:genetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000032:analysis; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives; D008099:Liver / Q000032:analysis; D012343:RNA, Transfer / Q000302:isolation & purification*; D051381:Rats
chemical_list: D000596:Amino Acids; C026376:Q nucleotide; D006157:Guanosine Monophosphate; D012343:RNA, Transfer