The mechanism of adenosine production in rat polymorphonuclear leucocytes.
Adenosine production in intact rat polymorphonuclear leucocytes was studied during 2-deoxyglucose-induced ATP catabolism. A cell-free system containing the cytosolic 5'-nucleotidase (EC 3.1.3.5) as the only phosphohydrolase was also studied. The rate of adenosine formation in both intact cells and the cell-free system showed a similar dependence on energy charge (([ATP] + 1/2 [ADP]/([ATP] + [ADP] + [AMP])), being maximal only at values close to 0.8. Sufficient cytosolic 5'-nucleotidase was present in intact cells to explain the observed rate of adenosine formation. We conclude that the cytosolic 5'-nucleotidase is responsible for adenosine production in rat polymorphonuclear leucocytes. This mechanism provides a direct biochemical link between the energy status of a cell and the rate of adenosine formation.
D015720:5'-Nucleotidase; D000241:Adenosine / Q000096:biosynthesis*; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002474:Cell-Free System; D003600:Cytosol / Q000201:enzymology; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000096:biosynthesis; D007291:Inosine Monophosphate / Q000097:blood; D008099:Liver / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats
D007042:Hypoxanthines; D007291:Inosine Monophosphate; D019271:Hypoxanthine; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000241:Adenosine