A third defective lambdoid prophage of Escherichia coli K12 defined by the lambda derivative, lambdaqin111.
We describe the isolation and characterization of a new Q-independent substitution mutant of lambda, lambdaqin111, which differs from other characterized Q-independent lambda phages. This mutant defines a new lambda-like prophage in the bacterial chromosome, as seen by homologous recombination between lambdaqin111 and the host DNA and by DNA/DNA hybridization methods. Genetic and electron microscopy data show that this new prophage carries, at least, genes analogous to Q-S-R of lambda and also a cos site functionally identical to lambda cos. It is located near 34 min on the Escherichia coli K12 map, i.e. in the same region but at a different site from the defective Rac prophage.
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral; D008242:Lysogeny; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D010957:Plasmids; D014775:Virus Activation
D004274:DNA, Recombinant; D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes