title: Antibodies against toluene diisocyanate protein conjugates. Three methods of measurement.
abstract: With the use of canine antisera against toluene diisocyanate (TDI)-dog serum albumin (DSA), techniques for measuring antibody against TDI-DSA were evaluated. The use of an ammonium sulfate precipitation assay showed suggestive evidence of antibody binding but high levels of TDI-DSA precipitation in the absence of antibody limit any usefulness of this technique. Double-antibody co-precipitation techniques will measure total antibody or Ig class antibody against 125I-TDI-DSA. These techniques are quantitative. The polystyrene tube radioimmunoassay is a highly sensitive method of detecting and quantitatively estimating IgG antibody. The enzyme linked immunosorbent assay is a rapidly adaptable method for the quantitative estimation of IgG, IgA, and IgM against TDI-homologous proteins. All these techniques were compared and results are demonstrated by using the same serum sample for analysis.
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D011232:Chemical Precipitation; D003485:Cyanates / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D008722:Methods; D013997:Time Factors; D014051:Toluene 2,4-Diisocyanate / Q000276:immunology*
chemical_list: D000906:Antibodies; D003485:Cyanates; D014051:Toluene 2,4-Diisocyanate; D000645:Ammonium Sulfate