Restriction analysis of DNA from Treponema pallidum, the causative agent of syphilis.
When purified DNA from pathogenic Treponema pallidum is digested with restriction endonucleases it results in the formation of discrete DNA fragments which range between 2.5 to 10 Kilobase pairs. No such precise fragmentation occurs with DNA isolated from nonpathogenic T. pallidum. The appearance of the discrete restriction fragments from the pathogenic T. pallidum DNA does not represent a contamination of satellite DNA from rabbit, the host in which the organism was propagated, but rather represents the presence of redundant DNA or DNA of less complexity in the pathogenic T. pallidum DNA preparation.
D002499:Centrifugation, Density Gradient; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D009693:Nucleic Acid Hybridization; D014210:Treponema pallidum / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes