Simultaneous staining of proteins during polyacrylamide gel electrophoresis in acidic gels by countermigration of Coomassie brilliant blue R-250.
A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after electrophoresis.
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D011506:Proteins / Q000302:isolation & purification*; D012394:Rosaniline Dyes
D003574:Cytochrome c Group; D006657:Histones; D011506:Proteins; D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R