[GTP-binding proteins of the retinal photoreceptor membranes: purification, antibodies].
A scheme of immunization of rabbits with partially purified GTP-binding proteins isolated from ox retinal photoreceptor membranes has been developed. Using double radial immunodiffusion, it was shown that rabbit antiserum interacts with the GTP-binding proteins of the retinal phosphoreceptor membranes of ox, pig and frog. The antibodies interaction with the GTP-binding proteins of ox retinal rod outer segments was followed by immunoelectrophoresis as well as by elimination of the activating effect of the non-hydrolyzable derivative of GTP, guanyl-5'-yl-imidodiphosphate on cyclic nucleotide phosphodiesterase in illuminated photoreceptor membranes and in their extracts. A similar effect was exerted by immunoglobulins from immunized animals on the enzyme activation by NaF. Purified immunoglobulins type G did not practically interact with the GTP-binding proteins of the photoreceptor membranes.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002417:Cattle; D019204:GTP-Binding Proteins; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010786:Photoreceptor Cells / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D011896:Rana temporaria; D011956:Receptors, Cell Surface / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine
D000906:Antibodies; D007106:Immune Sera; D007074:Immunoglobulin G; D011956:Receptors, Cell Surface; D010727:Phosphoric Diester Hydrolases; D019204:GTP-Binding Proteins
GTP-sviazyvaiushchie belkie fotoretseptornykh membran setchatki: ochistka, antitela.