Vascular angiotensin-converting enzyme activity in man and other species.
Angiotensin-converting enzyme (ACE) activity in blood vessels of different species was determined. ACE was solubilized by Nonidet P-40, and assayed by reversible phase high performance liquid chromatography. Approximately 98% ACE was recovered in the liquid phase by the use of the detergent. The ACE activity varied with chloride ion (Cl-) concentrations; the maximum activities in dog, human, monkey and rabbit tissues were obtained at the concentrations of 800, 600, 600 and 300 mmol/l respectively. The optimal Cl- concentration was quite similar in different tissues and plasma obtained from the same species. The ACE activity in the cerebral, mesenteric, pulmonary and renal arteries was in a range between 1.01 and 1.60 m-units/mg of protein in dogs and between 0.43 and 0.94 m-unit/mg of protein in monkeys. The activity in dog aortae was 0.20 +/- 0.02 m-unit/mg of protein, and the activity in aortic endothelial cells was 2.61 +/- 0.65 m-units/mg of protein. ACE activities in the dog lung, kidney cortex and cerebral cortex were 28.6 +/- 2.6, 15.7 +/- 3.0 and 3.5 +/- 0.6 m-units/mg of protein respectively. SA-446, a captopril-like ACE inhibitor, reduced the ACE activity in arteries in a dose-dependent manner. Vascular ACE appears to be concentrated in the endothelium and may contribute to regulate vascular muscle tone and local blood flow by a conversion of angiotensin I into II.
D015097:3-Mercaptopropionic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D001011:Aorta / Q000201:enzymology; D001808:Blood Vessels / Q000201:enzymology*; D002712:Chlorides / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004727:Endothelium / Q000201:enzymology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008251:Macaca; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D053778:Thiazolidines
D000806:Angiotensin-Converting Enzyme Inhibitors; D002712:Chlorides; D053778:Thiazolidines; D015097:3-Mercaptopropionic Acid; C033437:rentiapril; D007703:Peptidyl-Dipeptidase A