title: Induction and amplification of T-lymphocyte proliferative responses to periodate and soybean agglutinin by human adult vascular endothelial cells.
abstract: Human mononuclear phagocyte (M phi) populations were compared to adult human endothelial cells (HEC) for their respective abilities to influence the proliferative responses of purified human T lymphocytes to the mitogenic agents Na-m-periodate (IO-4), soybean agglutinin (SBA), or allogeneic cells. HEC and M phi were both capable of inducing proliferative responses of allogeneic T lymphocytes in mixed-lymphocyte culture. Under low cell density culture conditions, purified T-lymphocyte proliferative responses to IO-4 or SBA could be restored by addition of syngeneic M phi or HEC. At higher cell density culture conditions, proliferation of T cells to IO-4 could be amplified more by HEC than M phi. T-lymphocyte proliferative responses to SBA were amplified by addition of HEC but were suppressed by addition of M phi. These findings indicate that human adult HEC are unique and potent accessory cells for T lymphocytes. Furthermore, these findings demonstrate that accessory cell functions of HEC can be discriminated from those of M phi.
mesh_terms: D001808:Blood Vessels; D002448:Cell Adhesion; D004727:Endothelium / Q000276:immunology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010504:Periodic Acid / Q000494:pharmacology*; D010586:Phagocytes / Q000276:immunology; D037121:Plant Lectins; D013025:Glycine max; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D037102:Lectins; D037121:Plant Lectins; D010504:Periodic Acid