Serodiagnosis of viral hepatitis A: rise in antibody titre and evaluation of three methods for detecting early and late antibodies.
A serological investigation was made on patients with viral hepatitis A and individuals with a past history of this disease. Titration of antibody in sequential samples was found to be of no help in diagnosis. Separation of early (IgM) from late (IgG) antibodies by protein A or by 2-mercaptoethanol did not prove to be convenient for the serodiagnosis. A chromatographic separation of late and early antibody was found to be satisfactory, and equivalent to a radioimmunoassay for IgM-antibodies.
D000914:Antibodies, Viral / Q000032:analysis*; D005260:Female; D006506:Hepatitis A / Q000175:diagnosis*; D006507:Hepatovirus / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D012698:Serologic Tests; D013205:Staphylococcal Protein A
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A; D008623:Mercaptoethanol