Nuclear matrix-DNA complex resulting from EcoR1 digestion of HeLa nucleoids is enriched for DNA replicating forks.
Upon treatment of HeLa nucleoids with EcoR1 restriction enzyme, a minute fraction of the DNA remains attached to the nuclear matrix. This matrix-DNA complex appears to be composed of a unique set of proteins. Electron microscopic analysis of the DNA fragments present shows an enrichment for HeLa DNA replicating forks.Images
D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D008970:Molecular Weight
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI