The transforming gene of avian myeloblastosis virus (AMV): nucleotide sequence analysis and identification of its translational product.
The genome of the avian myeloblastosis virus (AMV) has undergone a sequence substitution in which a portion of the region normally coding for the env protein has been replaced by cellular sequences. We have determined the complete nucleotide sequence of this region. Examination of the AMV oncogenic sequence revealed an open reading frame starting with the initiation codon ATG and terminating with the triplet TAG within the acquired cellular sequences and terminating with the triplet TAG at a point thirty-three nucleotides into helper viral sequences to the right of the helper-viral-cellular junction. The stretch of 795 nucleotides would code for a protein of 265 amino acids with a molecular weight of 30,000 daltons. The eleven amino acids at the carboxy terminus of such a protein would be derived from the env gene of helper virus. Antibodies were prepared against synthetic peptides derived from the predicted amino acid sequences. One such antibody precipitated two magnesium proteins of apparent nucleotide weight of 30,000 daltons and 51,000 daltons.
D000595:Amino Acid Sequence; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics*; D009857:Oncogenes; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000235:genetics*
D009363:Neoplasm Proteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes