[Isolation of the specific endonuclease BamH1 in the multicyclic cultivation of Bacillus amyloliquefaciens].
The present article deals with the study of the multicyclic process of the cultivation of B. amyloliquefaciens in a liquid medium prepared from natural raw materials and with obtaining restrictase Bam H1 from the biomass with different microbiological characteristics. The selected medium has been found to be suitable for the realization of the multicyclic cultivation process on an industrial scale. The tendency towards a drop in the level of nonspecific nucleases simultaneously with a decrease in the specific growth rate has been revealed. A simplified scheme for the purification of the restricting endonuclease is proposed.
D000332:Aerobiosis; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D001431:Bacteriological Techniques; D002852:Chromatography, Ion Exchange; D003470:Culture Media / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000096:biosynthesis / Q000302:isolation & purification*; D015245:Deoxyribonuclease BamHI
D003470:Culture Media; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI
Poluchenie spetsificheskoĭ éndonukleazy BamH1 pri mnogotsiklicheskom kul'tivirovanii Bacillus amyloliquefaciens.