Transformation of ATLA-negative leukocytes by blood components from anti-ATLA-positive donors in vitro.
Anti-ATLA-positive blood components transformed healthy human leukocytes in vitro. Blood components examined were packed red cells, whole blood, platelet concentrate and fresh frozen plasma. Leukocytes present in anti-ATLA-positive blood components such as packed red cells, whole blood and platelet concentrate easily transformed anti-ATLA-negative leukocytes. Co-culture in fresh frozen plasma, however, did not transform recipient leukocytes, and leukocytes of anti-ATLA-positive recipients proved refractory to transformation. The transformed cells were morphologically lymphoid, grew in suspension, and possessed normal recipient karyotypes except in the case of three platelet concentrates. A high proportion of all the transformed populations formed E-rosettes with neuraminidase-treated sheep erythrocytes. The cytoplasm of over 90% of each recipient was stained brilliantly with antibodies against ATLV-determined antigens. Electron microscopy of these transformed cells revealed many C-type virus particles in the extracellular space. Blood components, such as packed red cells, whole blood and platelet concentrate, containing leukocytes from anti-ATLA-positive donors, should be used cautiously to prevent the transmission on ATLV to anti-ATLA-negative recipients.
D000328:Adult; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000097:blood* / Q000276:immunology / Q000382:microbiology; D007962:Leukocytes / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D012190:Retroviridae / Q000276:immunology; D012192:Retroviridae Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology; D013601:T-Lymphocytes
D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral