Fish oil administration as a supplement to a corn oil containing diet affects arterial prostacyclin production more than platelet thromboxane formation in the rat.
The administration to male rats of 5 en % fish oil (FO) as supplement to a diet containing 5 en % corn oil (CO), selectively and markedly decreased arterial parameters (6-keto-PGF1 alpha formation and platelet antiaggregatory activity) assessed in isolated aortic segments perfused with autologous platelet rich plasma (PRP). Platelet parameters (ADP-induced aggregation, TxB2 formation in thrombin-stimulated PRP and sensitivity to exogenous PGI2) were instead minimally affected. Eicosapentaenoic acid (EPA, 20:5 n-3) did not accumulate in plasma, platelet and aorta lipids and arachidonic acid (AA, 20:4 n-6) levels declined markedly only in the plasma compartment. When FO was given alone at the same 5 en % level, both arterial and platelet parameters were similarly affected. EPA accumulated in plasma cholesterol esters and was present in appreciable concentrations also in platelets and aortic walls. AA levels declined markedly in plasma lipids and appreciably also in platelet and aorta lipids. It is concluded that a) arterial and platelet parameters are differentially affected by FO administration depending upon the presence of n-6 polyunsaturated fatty acids in the diet, b) 6-keto-PGF1 alpha production by arterial tissue does not seem to be related to changes of PG precursor fatty acid levels in the phospholipid fraction.
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D003314:Corn Oil; D000242:Cyclic AMP / Q000097:blood; D004041:Dietary Fats / Q000494:pharmacology*; D011464:Epoprostenol / Q000096:biosynthesis* / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D005395:Fish Oils / Q000494:pharmacology*; D008297:Male; D009821:Oils / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D013929:Thromboxane B2 / Q000096:biosynthesis* / Q000097:blood; D013931:Thromboxanes / Q000096:biosynthesis*
D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D009821:Oils; D011453:Prostaglandins; D013931:Thromboxanes; D013929:Thromboxane B2; D003314:Corn Oil; D011464:Epoprostenol; D000242:Cyclic AMP