Inhibition of erythrocyte ATPase activity by aclacinomycin and reverse effects of ascorbate on ATPase activity.
We studied the effect of aclacinomycin on human erythrocyte membrane enzymes. Aclacinomycin inhibited ATPase, including Na-K-dependent ATPase, ouabain insensitive ATPase and Ca-ATPase. However acetylcholinesterase was not inhibited by aclacinomycin. The ATPase activities were not inhibited by aclacinomycin if ascorbate was added to the incubation mixture. However other reducing agents, alpha-tocopherol, superoxide dismutase and catalase had no effect on ATPase activity. Ascorbate may protect membrane proteins and lipids from peroxidate damage.
D015250:Aclarubicin / Q000031:analogs & derivatives*; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D001205:Ascorbic Acid / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D009279:Naphthacenes / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D009279:Naphthacenes; C011157:aclacinomycins; D010042:Ouabain; D015250:Aclarubicin; D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D001205:Ascorbic Acid