title: Evidence for variation of supercoil densities among simian virus 40 nucleoprotein complexes and for higher supercoil density in replicating complexes.
abstract: The distribution of DNA topoisomers in intracellular simian virus 40 DNA was analyzed by gel electrophoresis. The results suggested that DNA extracted from 70S chromatin had a different superhelical density distribution as compared with the DNA obtained from virions or virion assembly intermediates. The heterogeneity of simian virus 40 viral DNA superhelical density at a late time after infection was partly due to increased virion production and partly due to the intrinsic heterogeneity of the superhelical density of DNA extracted from virions. Using two-dimensional gel electrophoretic analysis we also showed that simian virus 40 DNA templates used for DNA replication have a higher average superhelical density than the bulk of intracellular viral DNA.
mesh_terms: D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D002843:Chromatin / Q000032:analysis; D004261:DNA Replication; D004278:DNA, Superhelical / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D009698:Nucleoproteins / Q000032:analysis*; D013539:Simian virus 40 / Q000235:genetics*; D013698:Templates, Genetic; D014771:Virion / Q000032:analysis
chemical_list: D002843:Chromatin; D004278:DNA, Superhelical; D004279:DNA, Viral; D009698:Nucleoproteins; D002738:Chloroquine