title: Mechanism of poliovirus inactivation by cell-free filtrates of marine bacteria.
abstract: The mechanism of enterovirus inactivation by marine bacteria was investigated using poliovirus type 1 as a model virus and with strains of Pseudomonas and Vibrio isolated from the marine environment. Treatment of virus with cell-free filtrates from late log phase bacterial cultures produced alterations in the viral capsid as shown by a reduction in efficiency of adsorption to host cells, increased sensitivity to ribonuclease, and by the release of ribonucleic acid from the treated virions. Filtration of 14C-labelled, treated virus through 25-nm filters revealed that the majority of the isotope (85-96%) passed the filters, indicating extensive capsid disruption. However, the most rapid and pronounced change observed during virus inactivation was the loss of infectivity, suggesting that enzymatic degradation is not the first event in the poliovirus inactivation process by marine bacteria.
mesh_terms: D000327:Adsorption; D000818:Animals; D000898:Antibiosis; D002213:Capsid / Q000648:ultrastructure; D002460:Cell Line; D002522:Chlorocebus aethiops; D007668:Kidney; D008851:Micropore Filters; D017955:Poliovirus / Q000502:physiology; D011549:Pseudomonas / Q000502:physiology*; D012260:Ribonucleases / Q000494:pharmacology; D012623:Seawater; D014733:Vibrio / Q000502:physiology*; D014871:Water Microbiology
chemical_list: D012260:Ribonucleases