title: Does 2H2O also protect membrane-bound enzymes?
abstract: Thermal inactivation studies performed with several membrane-bound enzymes such as chitin synthase, cytochrome c oxidase, nucleotidase, as well as bacterial, mitochondrial and plasma membrane ATPases from yeast in H2O and 2H2O indicated that most of these enzymes could not be protected by 2H2O against thermal inactivation. Only the Escherichia coli ATPase, which is located at the surface of the membrane, and the cytochrome c oxidase were stabilized by heavy water.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology*; D003903:Deuterium / Q000494:pharmacology*; D017666:Deuterium Oxide; D004798:Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005615:Freezing; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014867:Water / Q000494:pharmacology*
chemical_list: D004798:Enzymes; D014867:Water; D003903:Deuterium; D000251:Adenosine Triphosphatases; D017666:Deuterium Oxide