[Kinetics of the immobilization of trypsin on activated silichrome].
Trypsin is studied for kinetics of its immobilization on the surface of a porous spheric inorganic carrier with the grafted aldehyde groups in the surface layer. This process is found to be controlled by the enzyme diffusion. It is shown possible to use a body of mathematics known for kinetics of physical adsorption on the porous adsorbents to describe kinetics of protein chemosorption on the analogous carriers. A simple method is suggested for plotting a kinetic curve of the enzyme immobilization on the matrix with any sizes of particles from the experimentally obtained kinetic curve of its binding on the carrier with a definite diameter of particles.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D058428:Silica Gel; D012822:Silicon Dioxide; D014357:Trypsin / Q000378:metabolism*
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D058428:Silica Gel; D012822:Silicon Dioxide; D014357:Trypsin
Kinetika immobilizatsii tripsina na aktivirovannom silokhrome.