[Determination of the enzymatic traits of anaerobic gram-negative nonsporulating bacteria].
The method requiring the use of a semi-liquid medium with the reduced content of protein hydrolysates and with bromo-thymol blue added as indicator and permitting the determination of sugar fermentation by bacteroids and fusobacteria is presented. The determination is made by a change in the color of the indicator and the intensity of microbial growth (opacity) after the incubation of the inoculated medium under free access of air. A study has been made for comparing the above method with the electrometric method for the determination of fermentative features in anaerobic microorganisms.
D000693:Anaerobiosis; D001439:Bacteroides / Q000201:enzymology; D001441:Bacteroides fragilis / Q000201:enzymology; D003013:Clostridium / Q000201:enzymology; D003016:Clostridium perfringens / Q000201:enzymology; D003470:Culture Media / Q000378:metabolism; D005673:Fusobacterium / Q000201:enzymology; D005675:Fusobacterium necrophorum / Q000201:enzymology; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000201:enzymology; D006863:Hydrogen-Ion Concentration
D003470:Culture Media
Opredelenie fermentativnykh priznakov u anaérobnykh gramotritsatel'nykh neobrazuiushchikh spor bakteriĭ.