Rapid, economical qualitative method for separation of aflatoxins B-1, B-2 & G-1, G-2 by dry column chromatography.
A good correlation of four components of aflatoxins was accomplished by using the dry column chromatography method. The decolorization process of interfering substances, by 0.01 N KOH and defatting the extract with petroleum ether yields a clean residue for DCC separation. It is clear that the dry column chromatography is a very simple and time-saving procedure for separation of aflatoxins. DCC columns are more economical than precoated 'thick layer' preparative plates and, in DCC, no large developing tanks need to be used. Hazards associated with the use of large volumes of flammable solvents are greatly reduced.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000302:isolation & purification*; D000537:Aluminum Oxide; D001231:Aspergillus flavus / Q000032:analysis*; D002273:Carcinogens / Q000302:isolation & purification; D002476:Cellophane; D002845:Chromatography / Q000379:methods*; D058428:Silica Gel; D012822:Silicon Dioxide
D000348:Aflatoxins; D002273:Carcinogens; C027955:aflatoxin G1; C029754:aflatoxin G2; D058428:Silica Gel; D012822:Silicon Dioxide; C029753:aflatoxin B2; D002476:Cellophane; D016604:Aflatoxin B1; D000537:Aluminum Oxide