A flow cytometric technique for quantitation of B-cell activation.
A flow cytometric method for enumeration of intracytoplasmic antibody-containing cells was developed. Flow cytometry was found to yield results comparable to traditional fluorescence microscopy in cells stimulated to produce intracytoplasmic antibody by either pokeweed mitogen or Epstein-Barr virus. This technique offers several advantages over traditional fluorescence microscopy, including objective measurement of fluorescence intensity and rapid analysis of large numbers of cells.
D000921:Antibody-Producing Cells / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003593:Cytoplasm / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation