Inhibition of T7 bacteriophage replication by a colicin Ib plasmid gene.
Clones containing fragments of the colicin Ib (ColIb) plasmid inserted into pBR322 have been found that inhibit the replication of T7 bacteriophage. Cells containing the whole ColIb plasmid grow T7 normally but cannot grow T7 protein kinase-negative mutants of T7. The cloned fragments inhibit not only the T7 protein kinaseless mutants but wild-type T7 as well. However, the whole plasmid can suppress the wild-type T7 inhibition caused by the cloned inhibiting genes. These results are consistent with a model in which a ColIb gene (pic) exists which can inhibit replication of T7 phage. A second gene (rpi) can repress the function of pic provided the rpi product is phosphorylated.
D001483:Base Sequence; D003001:Cloning, Molecular; D003087:Colicins / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D013045:Species Specificity; D013604:T-Phages / Q000235:genetics*; D014779:Virus Replication
D003087:Colicins; D004262:DNA Restriction Enzymes