title: Improved phage vector system in Streptomyces.
abstract: A phage vector system was established by use of R4 phage in Streptomyces. Increased transfection frequency, introduction of a single BamHI site as a possible cloning site, and simple and rapid methods for isolation of phage DNA were achieved for the establishment.
mesh_terms: D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D014442:Monophenol Monooxygenase / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D014162:Transfection
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D014442:Monophenol Monooxygenase; D004262:DNA Restriction Enzymes