Application of hydrophobic chromatography to the large scale purification of Desulfovibrio desulfuricans cytochrome C3.
Hydrophobic chromatography on Phenyl-Sepharose was employed for large scale purification of cytochrome c3 from Desulfovibrio desulfuricans. This chromatographic procedure minimizes operational volumes and eliminates the lengthy dialysis ordinarily used to remove large amounts of salts. Pure cytochrome c3 was obtained after one additional purification by ion-exchange chromatography.
D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003574:Cytochrome c Group / Q000302:isolation & purification*; D003901:Desulfovibrio / Q000201:enzymology*; D012685:Sepharose / Q000031:analogs & derivatives
D000596:Amino Acids; D003574:Cytochrome c Group; C020927:phenyl-sepharose; D012685:Sepharose; C017165:cytochrome c(3); D000645:Ammonium Sulfate