[Promoting action of tetradecanoylphorbol acetate on the phenotypic expression of the mutation for the acquisition of resistance to diphtheria toxin in cultured human cells].
Stable spontaneous mutants resistant to the protein synthesis inhibitor diphtheria toxin (DT) have been selected in human cell line eue at a very low frequency (8 x 10(-6)). We have studied in this system the effect of the promoting agent phorbol-12-myristate-13-acetate (TPA) on mutagenesis induced by benzo(a)pyrene (BP). TPA did not increase the mutation frequencies induced by BP, but it showed a trend to make shorter the expression time.
D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004167:Diphtheria Toxin / Q000494:pharmacology*; D004351:Drug Resistance; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D010641:Phenotype; D010704:Phorbols / Q000494:pharmacology*; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D001580:Benzopyrenes; D004167:Diphtheria Toxin; D010704:Phorbols; D001564:Benzo(a)pyrene; D013755:Tetradecanoylphorbol Acetate
Azione del promuovente TPA sull'espressione fenotipica della mutazione per l'acquisizione della resistenza alla tossina difterica in colture di cellule umane.