An evaluation of immune electron microscopy as a method for the quantitation of virus particles.
A comparison is made between poliovirus titres obtained by immune electron microscopy and those obtained by plaque assay. The former method yields smaller values which cannot be explained by inefficient sedimentation of virus-antibody clumps or incomplete settling on the grid. Using this comparison and immune electron microscopy the theoretical titre of hepatitis A virus can be estimated.
D000914:Antibodies, Viral / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis; D000937:Antigen-Antibody Reactions; D005243:Feces / Q000382:microbiology*; D035922:Hepatitis A Antibodies; D006507:Hepatovirus / Q000254:growth & development* / Q000276:immunology; D008854:Microscopy, Electron; D017955:Poliovirus / Q000254:growth & development* / Q000276:immunology; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D035922:Hepatitis A Antibodies