Abortive transformation of rat 3Y1 cells by simian virus 40: viral function overcoming inhibition of cellular proliferation under various conditions of culture.
Resting cultures of nonpermissive rat 3Y1 cells were infected with simian and T antigen expression and entry into S phase were examined under various conditions of culture. In the complete absence of serum from the medium or at an extremely high cell density, the cells delayed T antigen expression and entry into S phase, leaving the interval between the two events constant. Results using the viral mutants deleted in the coding region for the small t antigen ruled out the role of this antigen in induction of S phase. From these and other results presented, we conclude that the large T antigen induces S phase with the same efficiency under different conditions of cultures. We also present the evidence that the large T antigen function is required and is sufficient for entry into S phase in the second as well as in the first generation.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics* / Q000302:isolation & purification; D002453:Cell Cycle; D002460:Cell Line; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D007399:Interphase; D007700:Kinetics; D009154:Mutation; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology*
D000957:Antigens, Viral, Tumor