Enrichment of rat anterior pituitary cell types by metrizamide density gradient centrifugation.
Anterior pituitary cells derived from hypothyroid rats were enzymatically dispersed and then fractionated by equilibrium sedimentation through a metrizamide density gradient. Populations relatively enriched in thyrotrophs, somatotrophs, mammotrophs, or gonadotrophs were obtained. The thyrotroph-enriched fraction was put into short term culture and tested for hormone responsiveness. TRH stimulated TSH release with an ED50 of 8 X 10(-10) M, and this TRH effect was inhibited by T3 with an ED50 of 1.6 X 10(-11) M free T3. Metrizamide density gradient centrifugation is a useful technique for obtaining relatively enriched populations of viable anterior pituitary cell types.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D013006:Growth Hormone / Q000378:metabolism; D007037:Hypothyroidism / Q000473:pathology; D008297:Male; D008793:Metrizamide; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013972:Thyrotropin / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
D013973:Thyrotropin-Releasing Hormone; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone; D008793:Metrizamide