Heparin inhibits the activity of protein phosphatase-1.
Heparin inhibited the dephosphorylation of rabbit skeletal muscle or liver phosphorylase a by protein phosphatase-1. Other glycosaminoglycans (chondroitin sulfates) and their constituents were found to be without effect. The chromatography of a partially purified phosphatase preparation on heparin-Sepharose CL-6B resulted in a fraction that did not bind to the matrix and its activity was not inhibited by heparin or inhibitor-1. The phosphatase bound to heparin-Sepharose was eluted by 0.2 M NaCl and was inhibited by heparin or inhibitor-1.
D000818:Animals; D002352:Carrier Proteins; D002845:Chromatography; D006006:Glycogen Synthase / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010762:Phosphorylase a / Q000378:metabolism; D054645:Protein Phosphatase 1; D011506:Proteins / Q000494:pharmacology; D011817:Rabbits
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C021042:protein phosphatase inhibitor-1; D006493:Heparin; D010762:Phosphorylase a; D006006:Glycogen Synthase; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1