title: Organization of the left-hand end of the herpes simplex virus type 2 BglII N fragment.
abstract: We have determined the complete nucleotide sequence surrounding the region coding for a 38,000-dalton protein of herpes simplex virus type 2 strain 333 that is encoded between map coordinates 0.58 to 0.595 on the viral genome. The sequence data have revealed an open translational reading frame of 1,011 nucleotides encoding a protein of 337 amino acids. Upstream of those sequences, transcriptional regulatory signals could be identified that overlap another open reading frame of 489 nucleotides, which presumably encodes the carboxy-terminal 163 amino acids of a 140,000-dalton protein. Downstream of the gene encoding the 38,000-dalton protein a polyadenylation signal was found as well as one further along on the other strand near a termination codon that presumably punctuates the gene specifying a 61,000-dalton protein. The DNA sequence data were compared with the amended sequence of the herpes simplex virus type 1 strain KOS 40,000-dalton protein (K. G. Draper, R. F. Frink, and E. K. Wagner, J. Virol. 43:1123-1128, 1982) and with the 5' end of the same gene from herpes simplex virus type 1 strain 17 (J. McLaughlan and J. B. Clements, J. Gen. Virol. 64:997-1006, 1983) to assess the degree of inter- and intratypic variation. The comparison of the sequences has provided a basis for the type specifity of this viral protein and for different mechanisms giving rise to the diversity between herpes simplex virus types 1 and 2.
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli; D005814:Genes, Viral; D008970:Molecular Weight; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C024408:endodeoxyribonuclease SacI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific