Preparation of highly purified mitochondria of Neurospora crassa on a Percoll gradient.
Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity and a fine ADP/O ratio. Electrophoresis of nucleic acids demonstrated the absence of cytoplasmic rRNA.
D000244:Adenosine Diphosphate / Q000032:analysis; D002499:Centrifugation, Density Gradient; D003580:Cytochromes / Q000032:analysis; D003576:Electron Transport Complex IV / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008928:Mitochondria / Q000032:analysis* / Q000201:enzymology; D009491:Neurospora / Q000648:ultrastructure*; D009492:Neurospora crassa / Q000648:ultrastructure*; D012331:RNA, Fungal / Q000032:analysis
D003580:Cytochromes; D012331:RNA, Fungal; D000244:Adenosine Diphosphate; D007770:L-Lactate Dehydrogenase; D003576:Electron Transport Complex IV