title: Particulate cyclic 3',5'-nucleotide phosphodiesterase and calmodulin of cardiac muscle.
abstract: Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific. Phosphodiesterase activity occurring in the nuclear fraction could be further enriched by subfractionation on sucrose density gradients in the presence of MgCl2.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis*; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000032:analysis*; D000818:Animals; D002147:Calmodulin / Q000032:analysis*; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis; D004533:Egtazic Acid / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D009206:Myocardium / Q000032:analysis / Q000201:enzymology; D011189:Potassium Chloride / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D015636:Magnesium Chloride; D004533:Egtazic Acid; D011189:Potassium Chloride; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D008274:Magnesium