Opiatergic projection from the bed nucleus to the habenula: demonstration by a novel radioimmunohistochemical method.
In a modification of the indirect immunohistochemical method 125I-labeled secondary antibodies were used to autoradiographically visualize enkephalin-like immunoreactivity. Electrolytic lesions of the bed nucleus of the stria terminalis (BNST) resulted in a decrease in enkephalin-like immunoreactivity in the ipsilateral habenula. This suggests an opiatergic pathway originating in the BNST and projecting to the habenula. In addition, the value of the radioimmunohistochemical technique is discussed.
D000818:Animals; D001345:Autoradiography; D004723:Endorphins / Q000502:physiology*; D006651:Histocytochemistry; D007158:Immunologic Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission; D013788:Thalamus / Q000502:physiology*
D004723:Endorphins