Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus.
A guanidinium chloride extract of [3H]glucosamine- and [35S]methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003587:Cytomegalovirus / Q000032:analysis*; D006023:Glycoproteins / Q000302:isolation & purification; D008970:Molecular Weight; D014764:Viral Proteins / Q000302:isolation & purification*
D000596:Amino Acids; D006023:Glycoproteins; D014764:Viral Proteins