A possible role of "steroidogenic factor" in the corticoidogenic response to ACTH; effect of ACTH, cycloheximide and aminoglutethimide on the content of cholesterol in the outer and inner mitochondrial membrane of rat adrenal cortex.
The role of a "steriodogenic factor" in the corticoidogenic response to adrenocorticotropic hormone (ACTH) was studied in this experiment. ACTH caused an accumulation of cholesterol within the adrenocortical mitochondria in rat after pretreatment with either cycloheximide (CH) or aminoglutethimide (AG). The cholesterol distribution in these cholesterol-rich mitochondria was examined by measuring the cholesterol concentrations in the outer and inner mitochondrial membranes. In the case of pretreatment with CH, cholesterol was accumulated in the outer membrane and not in the inner membrane. In contrast, in the case of pretreatment with AG, cholesterol was accumulated in the inner membrane and not in the outer membrane. It could be concluded from the presented data that the function of a "steroidogenic factor" in the corticoidogenic response to ACTH might take place at the step of cholesterol translocation from the outer mitochondrial membrane to the inner membrane.
D000302:Adrenal Cortex / Q000187:drug effects / Q000648:ultrastructure*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000616:Aminoglutethimide / Q000494:pharmacology*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D005260:Female; D007425:Intracellular Membranes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000616:Aminoglutethimide; D000324:Adrenocorticotropic Hormone; D002784:Cholesterol; D003513:Cycloheximide