title: Deletion mutants of megacinogenic plasmid pBM309 from Bacillus megaterium.
abstract: A 46.8-kb plasmid, pBM309, of Bacillus megaterium determines the production of a bacteriocin, megacin A, and confers immunity against this antibiotic on the host cells. The megacin A (megA) and megacin A-immunity (megAim) genes were mapped on the physical map of pBM309 by using its deletion derivatives. Both genes were isolated as a 10.6-kb PstI fragment and cloned in Bacillus subtilis vector plasmid pBD9 for expression in B. megaterium.
mesh_terms: D001410:Bacillus megaterium / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics*; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008530:Megacins / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D010957:Plasmids
chemical_list: D001430:Bacteriocins; D008530:Megacins; D004262:DNA Restriction Enzymes