Localization of the major dehydrogenases in two methylotrophs by radiochemical labeling.
The localization of prominent proteins in intact cells of two methylotrophic bacteria, Hyphomicrobium sp. strain X and bacterium W3A1, was investigated by radiochemical labeling with [14C]isethionyl acetimidate. In bacterium W3A1, trimethylamine dehydrogenase was not labeled by the reagent and is, therefore, an intracellular protein, whereas the periplasmic location of the methylamine and methanol dehydrogenases was evidenced by being readily labeled in intact cells. Similarly, an intracellular location of the trimethylamine and dimethylamine dehydrogenases in Hyphomicrobium sp. strain X was indicated, whereas methanol dehydrogenase was periplasmic.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D003574:Cytochrome c Group / Q000032:analysis; D003593:Cytoplasm / Q000201:enzymology; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000648:ultrastructure; D007096:Imidoesters / Q000378:metabolism; D007513:Isethionic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis*; D010089:Oxidoreductases, N-Demethylating / Q000032:analysis*
D003574:Cytochrome c Group; D007096:Imidoesters; C020761:isethionyl acetimidate; D007513:Isethionic Acid; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase; C020064:dimethylamine dehydrogenase