Production and purification of human MG63 cell interferon.
MG63 cells induced by Sendai virus were a convenient source of human interferon, which was of the beta type in antigenicity. When analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the interferon migrated as a single component with a molecular weight of 22,000 daltons. It was purified to apparent homogeneity by chromatography on Blue Sepharose, followed by SDS-PAGE, and estimated to have a specific activity of 4 x 10(8) international units/mg of protein.
D002460:Cell Line; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D012516:Osteosarcoma; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology
D007370:Interferon Type I; D011070:Poly I-C