Identification and quantitation of arachidonic-acid metabolic products in rabbit, rat and human saliva.
Arachidonic-acid metabolites were identified in salivary secretions of rabbits, rats and man and in homogenates of rabbit parotid and submandibular glands. The following levels were determined in mixed saliva of healthy volunteers: PGE2, 0.65 +/- 0.8 ng/ml; PGF2 alpha, 0.41 +/- 0.06 ng/ml; serologically active hydroxyeicosatetraenoic acids (HETE) 319.6 +/- 36.6 ng/ml; serologically active 6-sulphidopeptide-containing leukotrienes 1.72 +/- 0.21 ng/ml (all values mean +/- SEM). In all instances, HETE markedly predominated over all other arachidonic-acid metabolites.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost; D015232:Dinoprostone; D004702:Endocrine Glands / Q000032:analysis; D005088:Exocrine Glands / Q000032:analysis; D005260:Female; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis; D008297:Male; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis*
D001095:Arachidonic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D007975:Leukotriene B4; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015237:Dinoprost; D015232:Dinoprostone