title: Insertion of apocytochrome c into lipid vesicles.
abstract: Apocytochrome c (cytochrome c without the heme) is synthesized in the cell cytoplasm without a cleaved signal sequence, then transported across the outer mitochondrial membrane. We have studied the interaction of apocytochrome c with lipid vesicles as a model for understanding protein translocation across membranes. Apocytochrome c (but not holocytochrome c) that has been incubated with vesicles at 37 degrees C in 0.2 M NaCl binds to the vesicles. Under these conditions, as well as upon incubation with detergent or at high protein concentrations, all the added protein remains partly accessible to externally added protease, but a COOH-terminal fragment of some of the protein molecules becomes protected against digestion. When apocytochrome c is added to azolectin vesicles with internally trapped proteases, most of the added protein can be digested, even in the presence of a large excess of protease inhibitor external to the vesicles. Thus, in spite of a lack of nonpolar stretches in its amino acid sequence, apocytochrome c is capable of binding to and inserting into lipid membranes. In this model system, transport may be driven by trapping of protease-digested apocytochrome c on one side of the membrane.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001059:Apoproteins / Q000302:isolation & purification / Q000378:metabolism*; D003574:Cytochrome c Group / Q000302:isolation & purification / Q000378:metabolism*; D045304:Cytochromes c; D006736:Horses; D008081:Liposomes; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding
chemical_list: D000596:Amino Acids; D001059:Apoproteins; D003574:Cytochrome c Group; D008081:Liposomes; D010446:Peptide Fragments; D045304:Cytochromes c