Marek's disease herpesviruses. IV. Molecular characterization of Marek's disease herpesvirus A antigen.
Marek's disease herpesvirus A antigen (MDHV-A) was identified as a 61,000- to 65,000-dalton glycoprotein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation from the culture medium of both [35S]methionine- and [14C]glucosamine-labeled infected cells by specific rabbit serum directed against MDHV-A. Rigorous identification was accomplished by selective blocking of this specific immunoprecipitation of the glycoprotein with purified MDHV-A that was isolated at its characteristic isoelectric point. These results identify and characterize MDHV-A in terms of the previously determined physical and chemical properties of the antigen. A molecule of similar size was immunoprecipitated from the culture medium of cells infected with herpesvirus of turkeys, extending previous observations about the identity of a potentially important common antigen shared by MDHV and the nonpathogenic vaccine virus, herpesvirus of turkeys.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian; D005347:Fibroblasts / Q000276:immunology; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D007158:Immunologic Techniques; D007526:Isoelectric Point
D000956:Antigens, Viral; D003470:Culture Media