Characterization of NADPH-cytochrome P-450 reductase in a mouse hepatoma cell line.
NADPH-cytochrome c reductase in Hepa-1 cells was induced 2-fold by phenobarbital, but was not induced by benz[a]anthracene or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The apparent Km of the enzyme for NADPH was 0.57 microM; the activity was inhibitable by NADP; and segregated primarily to the microsomal fraction. Cytoplasm of Hepa-1 cells bound antibody to rabbit cytochrome P-450 reductase. 3T3 cells, which possessed one sixth of the cytochrome c reductase activity of Hepa-1 cells, bound correspondingly less cytochrome P-450 reductase antibody. This supports the notion that cytochrome P-450 reductase was responsible for the cytochrome c reductase activity that was measured.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002460:Cell Line; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008113:Liver Neoplasms / Q000201:enzymology*; D051379:Mice; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000201:enzymology; D010634:Phenobarbital / Q000494:pharmacology; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats
D001551:Benz(a)Anthracenes; D000072317:Polychlorinated Dibenzodioxins; C035667:1,3,7,9-tetrachlorodibenzo-p-dioxin; C030935:benz(a)anthracene; D001189:Aryl Hydrocarbon Hydroxylases; D009251:NADPH-Ferrihemoprotein Reductase; D010634:Phenobarbital