[Effect of the tumor promotor 12-o-tetradecanoyl phorbol-13--acetate on cyclic adenosine monophosphate levels in cell cultures. Relation to cell proliferation].
A considerable increase in the level of cyclic adenosine monophosphate (cAMP) was found in hepatoma cultures (clones G-10, G-1c) and L-cells (clones Lebr 625, Lebr f. s.) in 30, 60 and 120 minutes after their treatment with the tumor promoter 12-o-tetradecanoyl-phorbol-13-acetate (TPA). In the mutant cells with changed membranes (clones G-1c, Lebr 625, Lebr 625 f. s.) the rising of cAMP was less expressed under the influence of TPA. The contents of cAMP decreases to the control level by 22 hours after their TPA treatment. A consequence of biochemical changes, leading to the tumor growth after TPA treatment of the cells, has been proposed. A considerable increment in cAMP amount is supposed to be a trigger in this chain.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004351:Drug Resistance; D007739:L Cells / Q000187:drug effects* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D010704:Phorbols; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate
Vliianie opukholevogo promotora 12-o-tertadekanoil-fornbol-13-atsetata na uroven' tsiklicheskogo adenozinmonofosfata v kletochnykh kul'turakh. Sviaz' s proliferatsieĭ kletok.