ATP is required for initiation of poliovirus RNA synthesis in vitro: demonstration of tyrosine-phosphate linkage between in vitro-synthesized RNA and genome-linked protein.
Poliovirus replicase- and host factor-catalyzed copying of 3'-terminal polyadenylic acid [poly(A)] of poliovirion RNA was studied. Host factor-stimulated synthesis of polyuridylic acid [poly(U)] by the replicase required ATP in addition to UTP. ATP was not required for the oligouridylic acid-primed copying of 3'-terminal poly(A) of virion RNA. GTP, CTP, and AMP-PCP (5'-adenylyl beta-gamma methylenediphosphate, an ATP analog) could not replace ATP in host factor-stimulated synthesis of poly(U). Antibodies to poliovirus genome-linked protein (VPg) specifically precipitated in vitro-synthesized poly(U) from a host factor-stimulated reaction. The poly(U) synthesized in a host factor-stimulated reaction was shown to be attached to VPg precursor polypeptide(s) via a tyrosine-phosphate bond as found in poliovirion VPg-RNA.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D005814:Genes, Viral; D006160:Guanosine Triphosphate / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D034982:Integration Host Factors; D007700:Kinetics; D009713:Nucleotidyltransferases / Q000378:metabolism; D017955:Poliovirus / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D011072:Poly U / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism; D014158:Transcription, Genetic; D014443:Tyrosine / Q000032:analysis*; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D002352:Carrier Proteins; D034982:Integration Host Factors; D012367:RNA, Viral; D014758:Viral Core Proteins; D014764:Viral Proteins; D011061:Poly A; D011072:Poly U; D014443:Tyrosine; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D009713:Nucleotidyltransferases; C022428:poly U polymerase; D012324:RNA-Dependent RNA Polymerase