Changes in actin associated with the cytoskeleton following chemotactic stimulation of dictyostelium discoideum.
Chemotactic stimulation of Dictyostelium discoideum amoebae with pulses of cAMP or folate causes a series of rapid changes in the amount of actin protein associated with the Triton-insoluble cytoskeleton. The first of these changes occurs within 3 sec. of stimulation. The changes are dose-dependent and are within the physiological range of concentrations of cAMP or folate eliciting chemotaxis. These effects on the cytoskeleton show a pattern of regulation during development matching the respective chemotactic sensitivities of D.discoideum to cAMP (most sensitive at 4-8 hr of development) and to folate (rapidly decreasing sensitivity over 0-4 hr). At twelve hr, however, the responsiveness to folate unexpectedly reappears, suggesting a function of folate later in development than previously reported.
D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D002633:Chemotaxis / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology*; D003902:Detergents; D004023:Dictyostelium / Q000187:drug effects / Q000502:physiology*; D005492:Folic Acid / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility
D000199:Actins; D003902:Detergents; D011092:Polyethylene Glycols; D017830:Octoxynol; D005492:Folic Acid; D000242:Cyclic AMP