Synthesis and intracellular transport of cytochrome oxidase subunit IV and ADP/ATP translocator protein in intact hepatoma cells.
The biosynthesis of two mitochondrial membrane proteins - subunit IV of cytochrome oxidase and ADP/ATP translocator protein was studied in intact ascites hepatoma cells. Using pulse-chase labeling and rapid cell fractionation it was possible to identify the precursoric forms of these inner mitochondrial membrane proteins. It was found that the subunit IV of cytochrome oxidase is synthesized in the cytoplasm of mammalian cells in the form of a larger precursor while ADP/ATP translocator protein is synthesized in the form that is electrophoretically undistinguishable from the mature membrane integrated form.
D000818:Animals; D000936:Antigen-Antibody Complex; D001692:Biological Transport; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000378:metabolism*; D007106:Immune Sera; D007425:Intracellular Membranes / Q000032:analysis; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000032:analysis; D008928:Mitochondria / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000096:biosynthesis / Q000378:metabolism*; D008970:Molecular Weight; D009713:Nucleotidyltransferases / Q000378:metabolism*; D051381:Rats
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D046911:Macromolecular Substances; D008565:Membrane Proteins; D000226:Mitochondrial ADP, ATP Translocases; D003576:Electron Transport Complex IV; D009713:Nucleotidyltransferases