The effect of specific antibodies on oxygen uptake and H+ pumping by cytochrome c oxidase vesicles.
Antibodies to solubilized cytochrome c oxidase and to subunit III were incubated with liposomal oxidase. In oxygen uptake experiments, the inhibiting effects on RCI of anti-oxidase (primarily anti- subunits II and IV) and anti-III were by different mechanisms: the former, by inhibiting the uncoupled rate; the later, by stimulating the coupled rate. In experiments with H+ translocation, anti-oxidase was without effect, while anti-III was a potent inhibitor of proton pumping. These results are conclusive evidence for redox-linked proton extrusion from the vesicles by the oxidase (and its subunit III).
D000906:Antibodies; D000918:Antibody Specificity; D001693:Biological Transport, Active; D003576:Electron Transport Complex IV / Q000276:immunology*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011522:Protons; D012995:Solubility
D000906:Antibodies; D011522:Protons; D003576:Electron Transport Complex IV