Tripeptide analysis of collagen synthesized by silica-exposed fibroblast cultures.
The effect of silica on collagen biosynthesis by confluent monolayers of WI-38 fibroblast cultures was examined by a more comprehensive method of analysis. The presence of the particulates had no direct effect on protein (collagen) synthesis, proline incorporation or prolyl hydroxylase activity; the latter is determined by the degree of hydroxylation. Silica, however, was highly toxic to the cells.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis*; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology*
D010446:Peptide Fragments; D012822:Silicon Dioxide; D003094:Collagen; D011393:Procollagen-Proline Dioxygenase