title: The subcellular localization of calmodulin, cyclic AMP phosphodiesterase, and adenylate cyclase in bovine adrenal medulla.
abstract: The subcellular localization of calmodulin, cyclic nucleotide phosphodiesterase, and adenylate cyclase was studied in bovine adrenal medulla. Approximately 70% of the calmodulin and 90% of the cAMP phosphodiesterase activities were found colocalized in the cytoplasm. The subcellular distribution of adenylate cyclase closely paralleled the distribution of acetylcholinesterase, a marker for plasma membranes. The fraction of calmodulin which is particulate in nature has a distribution profile very similar to that of adenylate cyclase. The chromaffin granule fraction contained only 0.86% of the total cAMP phosphodiesterase, 0.41% of the total adenylate cyclase, and 1.4% of the total calmodulin.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis*; D000262:Adenylyl Cyclases / Q000032:analysis*; D000313:Adrenal Medulla / Q000032:analysis* / Q000201:enzymology; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002147:Calmodulin / Q000032:analysis*; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D011506:Proteins / Q000032:analysis; D013347:Subcellular Fractions / Q000032:analysis / Q000201:enzymology
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin; D011506:Proteins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases