Cellular origin of the chemokinetic inhibitor of polymorphononuclear leucocytes found in sera from patients with chronic lymphocytic leukaemia.
In a previous study, we demonstrated the existence in serum from patients with chronic lymphocytic leukaemia (CLL) of a cell-directed, heat-labile chemokinetic inhibitor of polymorphonuclear leucocytes (PMN). The aim of this investigation was to study the cellular origin of this inhibitor. Supernatants from short term cultured lymphocytes from 3 healthy persons and 5 patients with CLL, and from 16 different established human non-haematopoietic and haematopoietic cell lines were analyzed. Only the leukaemic B-lymphocytes from the CLL patients produced the inhibitor. The presence of the inhibitor was also demonstrated in supernatants from the fresh CLL cells from one CLL patient and a leukaemic cell line established from these cells using Epstein-Barr virus as the immortalizing agent. A lymphoblastoid cell line derived from the normal B-cells of the same CLL patient did not produce the chemokinetic inhibitor. We therefore conclude that the cellular origin of the heat-labile, cell-directed chemokinetic inhibitor of PMN migration is the CLL cell.
D001402:B-Lymphocytes / Q000502:physiology; D018288:Carcinoma, Small Cell / Q000503:physiopathology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000503:physiopathology; D007945:Leukemia, Lymphoid / Q000097:blood* / Q000503:physiopathology; D008175:Lung Neoplasms / Q000503:physiopathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000503:physiopathology; D008297:Male; D009504:Neutrophils / Q000502:physiology*