Oxidation of alpha-tocopherol in micelles and liposomes by the hydroxyl, perhydroxyl, and superoxide free radicals.
Rates of oxidation of alpha-tocopherol by the hydroxyl- and superoxide free radicals were measured. The radicals were produced in known yields by radiolysis of aqueous solutions with gamma rays. Two main systems were used to dissolve the tocopherol; micelles, made up from charged and uncharged amphiphiles, and membranes made from dimyristyl phosphatidylcholine which could be charged by addition of stearyl amine or dicetyl phosphate. The HO. radicals were efficient oxidants of alpha-tocopherol in all systems, with up to 83% of radicals generated in micelle and 32% in membrane suspensions initiating the oxidation. The HO.(2) radical was an even more effective oxidant, but when most of it was in the O(2) form at neutral or alkaline pH, the oxidation rates became low. Tocopherol held in positively charged micelles or membranes was oxidized at a higher rate by the O(2) than in uncharged or negative particles. Possible biological significance of these results is discussed.
D003102:Colloids; D004134:Dimyristoylphosphatidylcholine; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D007700:Kinetics; D008081:Liposomes; D008823:Micelles; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D013481:Superoxides; D014810:Vitamin E / Q000528:radiation effects
D003102:Colloids; D005609:Free Radicals; D006878:Hydroxides; D008081:Liposomes; D008823:Micelles; D013481:Superoxides; D014810:Vitamin E; D017665:Hydroxyl Radical; D004134:Dimyristoylphosphatidylcholine