title: Microinjected simian virus 40 cRNA is spliced, as evidenced by electron microscopy.
abstract: Simian virus 40 cRNA was transcribed in vitro from the early viral DNA strand. The RNA was injected through glass capillaries into the nuclei of monkey cells. After a 2-h incubation, the RNAs were extracted and hybridized to single-stranded simian virus 40 DNA sequences contained in a bacteriophage M13 vector. Electron microscopy revealed processed cRNAs with splice loops in the region of the intron of large T antigen.
mesh_terms: D000818:Animals; D002460:Cell Line; D000882:Haplorhini; D008845:Microinjections; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D012326:RNA Splicing; D012367:RNA, Viral / Q000378:metabolism*; D013539:Simian virus 40 / Q000032:analysis*
chemical_list: D012367:RNA, Viral