Establishment of a Ph1 chromosome-positive cell line from chronic myelogenous leukemia in blast crisis.
A new hematopoietic cell line, designated KCL-22, was established in vitro by cultivation of pleural effusion cells obtained from a woman with chronic myelogenous leukemia in blast crisis. KCL-22 grew in suspension culture with a doubling time of 24 h and consisted of immature undifferentiated cells which were positive for periodic acid-Schiff and acid phosphatase staining. Chromosome analysis of the KCL-22 line showed a female karyotype with double Ph1 chromosomes and additional chromosome abnormalities. Its representative karyotype was 52,XX, + 1p-,+6,+8,+8,+8, t(9q+;22q-), +22q-. This cell line possessed receptors for the Fc portion of IgG, but lacked lymphoid cell characteristics and the Epstein-Barr virus-associated nuclear antigen. These results indicate that the KCL-22 cells were derived from chronic myelogenous leukemia cells. This cell line should prove useful for research involving various aspects of chronic myelogenous leukemia.
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002904:Chromosomes, Human, 21-22 and Y; D003593:Cytoplasm / Q000648:ultrastructure; D004253:DNA Nucleotidylexotransferase / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens; D005169:Factor VIII / Q000032:analysis; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D010587:Phagocytosis; D010996:Pleural Effusion; D011971:Receptors, Immunologic
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D011971:Receptors, Immunologic; D005169:Factor VIII; D005340:Fibrinogen; D004253:DNA Nucleotidylexotransferase