Effect of heat treatment of dietary fiber: interlaboratory study.
The effects of thermal treatments on the dietary fiber composition of cereal and potato samples were studied at 8 laboratories using different analytical methods. Thermal treatments included extrusion cooking for cereals, and boiling and frying for potatoes. No changes in the amounts of dietary fiber or starch were observed in the extruded samples. Heat-treated potato samples contained significantly more water-insoluble dietary fiber (cellulose) and less starch than did raw potato. However, this may be due, at least in part, to the sample preparation procedure rather than the heat treatment alone. The study indicates that gravimetric and sequential hydrolysis methods give similar results, but the variation in most cases is still wide. Further standardization of methods is evidently needed, especially if legislative measures for setting limits on the fiber content of foods are to be introduced. Standardization of starch analysis should also be of primary importance in future work on carbohydrate methodology. This can be concluded from the variation in results concerning this extremely important food constituent. It should be noted that the sample matrix in the present study was simpler than that of complex meals or diets.
D004043:Dietary Fiber / Q000032:analysis*; D006358:Hot Temperature; D014908:Triticum / Q000032:analysis; D014675:Vegetables / Q000032:analysis