Use of inter-proton nuclear Overhauser effects to assign the nuclear magnetic resonance spectra of oligodeoxynucleotide and hybrid duplexes in aqueous solution.
Inter-proton nuclear Overhauser enhancements (NOEs) have been used to assign the aromatic, anomeric and 2' resonances in the 1H nuclear magnetic resonance spectrum of the duplex formed between d(T-C-A-C-A-T) and d(A-T-G-T-G-A). The same techniques have been applied to assignments in the hybrid duplex formed by d(T-C-A-C-A-T) with r(A-U-G-U-G-A). Comparison of intra-residue with inter-residue NOEs yields structural information which suggests that the conformations of both duplexes are similar. The NOEs are consistent with inter-proton distances measured from models of B-form DNA. Circular dichroic results confirm these deductions.
D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011522:Protons; D012996:Solutions
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011522:Protons; D012996:Solutions