Cytochemical glucose-6-phosphatase activity in the cells of mouse pancreas and submandibular gland.
Ultrastructural localization of glucose-6-phosphatase activity was studied in the cells of the pancreas and submandibular gland of the mouse using a incubation medium modified from that of Wachstein & Meisel (1956). In pancreatic acinar cells, the reaction product for the enzyme activity was not found even after 90 min of incubation with three changes of the medium. However, the reaction product was localized in the endoplasmic reticulum and nuclear envelope of all other cell types composing the pancreas and submandibular gland. The reaction product appeared in moderate to abundant amounts in acinar cells and striated duct cells of the submandibular gland, and in the B cells, A and D cells of the pancreatic islet, but it was scarce in other cell types.
D000818:Animals; D002101:Cacodylic Acid / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005952:Glucose-6-Phosphatase / Q000032:analysis* / Q000502:physiology; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010179:Pancreas / Q000201:enzymology*; D013363:Submandibular Gland / Q000201:enzymology*
D002101:Cacodylic Acid; D005952:Glucose-6-Phosphatase