A cell surface differentiation antigen involved in human neutrophil chemotaxis and degranulation.
In this study we have demonstrated that the human promyelocytic leukaemia cell line (HL-60 cells) completely lack the biological functions of chemotaxis and degranulation. In addition, they were also unable to bind the anti-neutrophil monoclonal antibody NCD 1 which has been shown to inhibit these functions in the peripheral blood neutrophil. When HL-60 cells were induced to differentiate by culturing for 6 days at 37 degrees C in the presence of either 1.25% dimethylsulphoxide (DMSO) or 0.5% dimethylformamide (DMF), up to 35% bound NCD 1. Differentiated HL-60 cells are capable of chemotaxis, degranulation and these newly acquired functions were inhibited by NCD 1 in a manner similar to that seen for the peripheral blood neutrophil. The results correlate the appearance of an antigen on the cell surface of DMSO- or DMF-induced HL-60 cells with the acquisition of two specific cellular functions.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D002634:Chemotaxis, Leukocyte; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009195:Peroxidase