Point mutations of the adenovirus major late promoter with different transcriptional efficiencies in vitro.
Synthetic oligodeoxyribonucleotides were used to introduce single base changes into the TATAA box of the adenovirus-2 major late promoter. The resultant mutant DNA templates that contained altered TATAA sequences (TATAA leads to TCTAA/TATCA) were tested for their relative transcriptional activity in vitro using HeLa whole cell extracts. The data indicate that the change TATAA leads to TCTAA results in a level of specific transcription reduced to 48.7% of that from the parental template, whereas the change TATAA leads to TATCA reduced transcription to 21%. Neither base change abolished promoter activity.
D000256:Adenoviridae / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D009876:Operon; D013045:Species Specificity; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes