Interleukin 2-independent stimulation of rabbit chondrocyte collagenase and prostaglandin E2 production by an interleukin 1-like factor.
In the present study, interleukin 1 (IL 1)-containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2 secretion by chondrocytes. A high correlation was found between the enhancement of thymocyte proliferation and the induction of collagenase and PGE2 secretion by chondrocytes. Furthermore, a highly purified IL 1-like factor, namely mononuclear cell factor (MCF) was also active on chondrocytes. The addition of highly purified IL 2 to rabbit chondrocytes had no effect on collagenase and PGE2 secretion induced by IL 1-containing media. Our findings suggest that the factor which induced collagenase and PGE2 secretion by rabbit chondrocytes was an IL 1-like factor. Thus, collagenase secretion by chondrocytes may be used as an IL 2-insensitive assay for the detection of IL 1-like factors.
D000818:Animals; D002356:Cartilage / Q000201:enzymology; D002478:Cells, Cultured; D003470:Culture Media; D015232:Dinoprostone; D004790:Enzyme Induction; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D003012:Microbial Collagenase / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis*; D011817:Rabbits
D003470:Culture Media; D007375:Interleukin-1; D007376:Interleukin-2; D011458:Prostaglandins E; D003012:Microbial Collagenase; D015232:Dinoprostone