Gamma-aminobutyrate as an autonomic neurotransmitter: release and uptake of [3H]gamma-aminobutyrate in guinea pig large intestine and cultured enteric neurons using physiological methods and electron microscopic autoradiography.
To provide further evidence that some enteric neurons use gamma-aminobutyrate (GABA) as a neurotransmitter, we have demonstrated a depolarization-induced release of [3H]GABA from isolated myenteric ganglia in culture, and from segments of large intestine containing the myenteric plexus. In addition, light and electron microscopic autoradiography has been employed to visualize the putative GABAergic neurons and their projections, both in cultured ganglia and in sections from the gut wall. Explant cultures of the guinea-pig myenteric plexus, containing only neurons and glia intrinsic to the gut, were incubated with 0.14 microM [3H]GABA, washed and then repeatedly depolarized by 62 mM K+. The depolarizations always evoked release of [3H]GABA. The evoked release was reversibly blocked by 5 mM Co2+, suggesting a dependence on Ca2+ influx, a characteristic of neuronal transmitter release. Strips of the guinea-pig taenia coli, containing the myenteric plexus, were incubated with 0.14 or 0.7 microM [3H]GABA, washed and subjected to electrical field stimulation. This caused release of [3H]GABA, which could be evoked successively on repeated stimulation. The release was of neuronal origin and Ca2+ dependent, since it was abolished by 3 microM tetrodotoxin and reversibly blocked by 10 mM Co2+. By combined electrophoresis and chromatography the released tritium was identified as being attached to GABA. Autoradiography, following incubation with low concentrations of [3H]GABA, was used to identify specifically putative GABAergic neurons. Light microscopic autoradiography of cultured ganglia, and electron microscopic autoradiography of sections from the taenia coli including the myenteric plexus, were in good agreement, showing a selective and heavy labelling over a sub-population of neurons, and light labelling over glial cells. The majority of neurons and the non-neural cells were unlabelled. The electron microscopic autoradiographs also showed heavy labelling over some, but not all, axons in the fine axon bundles that innervate the longitudinal muscle of the taenia. These results strongly support our previous suggestion that a population of myenteric neurons are GABAergic, although more work is needed before this is fully established. Our data also suggest that some of the putative GABAergic neurons project out to the gut musculature, where their axons, together with axons from other neurons, run in bundles to form the intramuscular nerves. We therefore propose that one of the roles of these neurons in the gut is the presynaptic modulation of transmitter released from neighbouring axons in these nerves.
D000818:Animals; D001341:Autonomic Nervous System / Q000378:metabolism*; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism; D003106:Colon / Q000294:innervation; D046508:Culture Techniques; D006168:Guinea Pigs; D007420:Intestine, Large / Q000294:innervation*; D008564:Membrane Potentials; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000294:innervation; D009197:Myenteric Plexus / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D009435:Synaptic Transmission; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D011188:Potassium; D002118:Calcium