Effects of 17 beta-estradiol and progesterone on the metabolism of benz[a]pyrene by rabbit endometrial cells in primary culture.
The direct effects of estrogen (17 beta-estradiol) and progesterone on the metabolism of [3H]benzo[a]pyrene (BP) by primary cultures of rabbit endometrium epithelial cells were studied. 17 beta-Estradiol (10(-7) M) did not affect the metabolism of [3H]BP, expressed as the formation of water soluble metabolites of [3H]BP, during a 24-h incubation period. However, progesterone (10(-7) M) inhibited the formation of water-soluble metabolites of [3H]BP and the inhibitory effect was not prevented by 17 beta-estradiol. Progesterone did not appear to affect the relative distribution of the major conjugate types (sulfate esters and glutathiones) that comprised the water-soluble metabolites of [3H]BP. The results, which showed that progesterone acted to inhibit the metabolism of BP by endometrial cells, suggest additional evidence of a role for the ovarian hormone in the modulation of the carcinogenic efficacy of chemical carcinogens in a hormone-responsive tissue.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D002478:Cells, Cultured; D004347:Drug Interactions; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D011817:Rabbits; D013431:Sulfates / Q000378:metabolism
D001580:Benzopyrenes; D013431:Sulfates; D001564:Benzo(a)pyrene; D011374:Progesterone; D004958:Estradiol; D005978:Glutathione