Physical analysis of the genomes of hybrid phages between phage P1 and plasmid p15B.
The genomes of three plaque-forming recombinant phages between phage P1 and plasmid p15B were characterized by restriction cleavage analysis and electron microscopic heteroduplex studies. The structure of all three P1-15 hybrid genomes differs from that of P1 DNA in the res mod region coding for restriction and modification systems EcoP15 and EcoP1, respectively. P1-15 hybrid 2 shows an additional major difference to P1 around the site of the residential IS1 element of P1 and it does not carry an IS1 in its genome.
D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D006824:Hybridization, Genetic; D008854:Microscopy, Electron; D009692:Nucleic Acid Heteroduplexes; D010957:Plasmids
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes