Rat testes interstitial cell nuclei exhibit three distinct receptors for retinoic acid.
Purified nuclei from rat testes interstitial cells were incubated with an equimolar complex of [3H]retinoic acid and purified cellular retinoic acid-binding protein (cRABP) and with ATP. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and radiofluorographic analysis of the nuclear fractions indicated the presence of 3 highly labeled receptors for retinoic acid which were distinct from cRABP. These data demonstrate that retinoic acid binds to 3 novel nuclear acceptors of which cRABP does not appear to be a part.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007985:Leydig Cells / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018168:Receptors, Retinoic Acid; D014212:Tretinoin / Q000378:metabolism*
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D000255:Adenosine Triphosphate