Transformation of NIH 3T3 cells by a human c-sis cDNA clone.
The mechanism of leukaemogenic transformation by human T-cell leukaemia/lymphoma virus (HTLV), a retrovirus implicated in the aetiology of certain adult T-cell leukaemias and lymphomas, is unknown but is conceivably associated with the expression of the cellular analogues of retroviral oncogenes. The HUT-102 cell line, derived from a cutaneous T-cell lymphoma and infected with HTLV, expresses several cellular oncogenes. It is unusual among haemopoietic cell lines in that one of these is c-sis, the gene from which the oncogene v-sis of the simian sarcoma virus was derived, and perhaps the gene for platelet-derived growth factor (PDGF). To explore the possible role of c-sis expression in HTLV-induced disease, we have obtained cDNA clones of c-sis from HUT-102 cells. Here we describe two such clones and report that one of them transforms NIH-3T3 cells. This is the first example of transformation of NIH-3T3 cells by a human onc gene other than c-ras or Blym, as well as the first demonstration of transformation by a human cDNA clone.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D017977:Deltaretrovirus / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000382:microbiology*; D051379:Mice; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D013601:T-Lymphocytes / Q000382:microbiology
D004273:DNA, Neoplasm; D004247:DNA; D004262:DNA Restriction Enzymes