title: Development and differentiation of the benzodiazepine receptor in cultures of fetal mouse spinal cord.
abstract: Cultures of fetal mouse spinal cord were assayed for the development of the benzodiazepine (BDZ) receptor on intact cells in situ. At 2 and 3 weeks in culture, levels of binding were low and Scatchard analysis was linear revealing a single binding site with Kds of 115 and 71 nM, respectively. After 4 weeks in culture, coincident with large increases in neuronal (clonazepam displaceable) and nonneuronal (Ro5-4864 displaceable) binding. Scatchard plots became non-linear revealing 2 distinct binding sites with Kds of 11 and 175 nM.
mesh_terms: D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D002454:Cell Differentiation; D002998:Clonazepam / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism; D005333:Fetus; D005445:Flunitrazepam / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009474:Neurons / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A; D013116:Spinal Cord / Q000166:cytology*
chemical_list: D001570:Benzodiazepinones; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D002998:Clonazepam; D005445:Flunitrazepam; D003975:Diazepam