Dexamethasone-stimulated expression of a proviral copy of mouse mammary tumor virus env mRNA.
The kinetics of dexamethasone induction of mouse mammary tumor virus RNAs in a cell line (designated line 8) which contains a deleted proviral DNA has been examined. This DNA is an apparent reverse transcription product of the env mRNA. A detectable increase in the 3.8-kilobase RNA (mouse mammary tumor virus env mRNA) was present within 15 min after hormone addition. The 2.5-kilobase RNA detected previously during hormone induction studies was also observed in these cells. Both RNAs increased in concentration up to about 4 h after hormone addition and thereafter attained the steady-state level. Pr73env was detected by immunoprecipitation and appeared to be identical to bona fide Pr73env isolated from wild-type mouse mammary tumor virus-infected cells. An additional 5.7 kilobases of RNA was detected in augmented quantities after hormone addition, but the structure of this RNA is not known.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes; D003907:Dexamethasone / Q000494:pharmacology*; D005796:Genes / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000302:isolation & purification
D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D003907:Dexamethasone; D004262:DNA Restriction Enzymes