Identification of porcine transmissible gastroenteritis virus in house flies (Musca domestica Linneaus).
Transmissible gastroenteritis (TGE) virus was detected in house flies (Musca domestica Linneaus) by staining with specific fluorescent antibody. The flies were collected within a swine confinement facility in which TGE was enzootic. Laboratory-reared flies were infected experimentally with TGE virus and the virus was recovered from the insects for 72 hours after infection. The TGE virus was identified both by the fluorescent antibody technique and by isolation in cell culture. The nature of plaque formation in cell monolayers inoculated with the virus passaged through flies changed from a large plaque (4 mm or greater in diameter) to a small plaque (1 mm in diameter) over the period. Large plaques were observed early after infection and were attributed to TGE virus mechanically carried by the flies. Small plaques occurred 8 to 12 hours after infection and were considered to be produced by virus replicated in the dipterous cell.
D000818:Animals; D000833:Animals, Suckling / Q000382:microbiology; D003332:Coronaviridae / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D005761:Gastroenteritis, Transmissible, of Swine / Q000382:microbiology / Q000635:transmission; D006793:Houseflies / Q000382:microbiology*; D007303:Insect Vectors / Q000382:microbiology*; D007484:Iowa; D012621:Seasons; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000302:isolation & purification*