title: [Endonuclease activity in the blood serum of rats undergoing nonuniform gamma irradiation].
abstract: During the first hours following nonuniform gamma-irradiation (the nonuniformity coefficient was 3:1 and 5:1 with a dose change along the axis directed cranio-caudally and caudo-cranially), endonuclease activity (pH 5.75) in the blood serum increased with the dose which was estimated with a reference to total utilization of the substrate. Supercoiled plasmid DNA served as the substrate. The transformation of a supercoiled form of plasmid DNA into an open circular form was the criterion of estimation of nuclease activity. It was shown that the increase in the nuclease activity correlated with the increase in the nonuniformity coefficient.
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004720:Endonucleases / Q000097:blood / Q000528:radiation effects*; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D008297:Male; D011833:Radiation Injuries, Experimental / Q000201:enzymology; D051381:Rats; D013997:Time Factors
chemical_list: D004720:Endonucleases
vernacular_title: Izuchenie éndonukleaznoĭ aktivnosti v syvorotke krovi krys pri neravnomernom gamma-obluchenii.