Purification of porcine granulosa cells by continuous Percoll gradient.
Granulosa cells purified on a continuous Percoll gradient have considerably increased responsiveness to FSH in formation of cAMP, secretion of progesterone, and 125I[hCG] binding to cells incubated for 4 days in culture.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003102:Colloids; D000242:Cyclic AMP / Q000032:analysis; D005260:Female; D006107:Granulosa Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D011205:Povidone; D011374:Progesterone / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D011974:Receptors, LH; D012822:Silicon Dioxide; D013552:Swine
D006063:Chorionic Gonadotropin; D003102:Colloids; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D011374:Progesterone; C016039:Percoll; D012822:Silicon Dioxide; D000242:Cyclic AMP; D011205:Povidone