Nucleotide sequence of a region downstream of the mouse CK immunoglobulin gene.
2318 bp downstream of the CK (1) gene segment were sequenced in a clone (L1-D) derived from mouse liver DNA. The 966 bp at the 5' side of this stretch were found to be identical to a sequence which had been determined previously in a myeloma T derived clone, i.e. no somatic mutations had occurred in the transition from the germline to the rearranged configuration. The remaining 1352 bp had not been known and extend the sequenced part of the mouse JK-CK region to about 7.5 kb. Within the newly sequenced area three BspRI sites have been located which were used in chromatin studies (Weischet et al., accompanying publications). In L1-D sequences have been found which are possible targets of aberrant recombination events.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D007136:Immunoglobulins; D008099:Liver / Q000276:immunology; D051379:Mice
D007136:Immunoglobulins; D004262:DNA Restriction Enzymes