title: Rat lymphoid cell lines with human T cell leukemia virus production. I. Biological and serological characterization.
abstract: Cocultivation of spleen cells, lymph node cells, and thymocytes of female Wistar-King-Aptekman rats with short-term cultured male adult T cell leukemia (ATL) cells in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) resulted in the establishment of rat lymphoid cell lines, TARS-1, TARL-2, and TART-1. Cytogenetic analysis of the three cell lines showed a female rat karyotype with 42 chromosomes. The surface phenotypes of TARS-1 and TART-1 were those of rat T cells. TARL-2 was only positive for rat Ia and leukocyte common antigens. The cell lines continuously produced a type C retrovirus, human T cell leukemia virus (HTLV) and expressed ATL-associated antigens. TARS-1 and TART-1, but not TARL-2 were transplantable into newborn syngeneic rats and nude mice. These results strongly indicate that HTLV not only immortalizes, but also transforms rat T cells in vitro. Adult rats immunized with either TARS-1 or TARL-2 produced antibodies specific for HTLV. The biochemical analysis of the antigens that reacted with rat sera revealed that they are the two HTLV-specific polypeptides, p24 and p28.
mesh_terms: D000368:Aged; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D017977:Deltaretrovirus / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007938:Leukemia / Q000209:etiology / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology; D014764:Viral Proteins / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000956:Antigens, Viral; D008562:Membrane Glycoproteins; D014764:Viral Proteins; C028388:thymus-leukemia antigens