Hepatocyte plasma membrane antigens. II. Characterization of liver-specific membrane lipoprotein (LP-1) and Tamm-Horsfall glycoprotein (THGP) like antigens (hepatic THGP) on the plasma membrane of Chang liver cell.
Radioiodinated cell-surface antigens of Chang liver cells recognized with anti-liver-specific membrane lipoprotein (anti-LP-1) and anti-Tamm-Horsfall glycoprotein (anti-THGP) rabbit antibodies were analyzed by the immunoprecipitation followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The analysis revealed that the antigens precipitated with anti-LP-1 were heterogeneous in molecular species consisting of 2 major and 5 minor polypeptides with molecular weights ranging from 36 X 10(3) (36 K) to 250 K daltons. That with anti-urinary THGP was a single polypeptide with a molecular weight of 67 K daltons, which was different from urinary THGP in molecular size, and was designated hepatic THGP.
D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface