An ELISA technique to detect IgG antibody to the early herpes simplex virus type 2 (HSV-2) antigen AG-4 in HSV-2 patients.
An ELISA system, based on urease activity was used for the detection and titration of IgG to the immediate early AG-4 antigen in sera from HSV-2 patients. It detected low levels of IgG to the AG-4 antigen in 32% of patients' sera known to contain complement fixing antibody to the antigen. Furthermore, the sensitivity of the ELISA system was 2- to 10-fold higher than the complement-fixation system depending on when the sera was taken from the HSV-2 patients. The system also allowed the easy detection and quantitation of AG-4 antigen production when various HSV-1 X HSV-2 intertypic recombinant viruses were used to infect BHK-21 cells.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006561:Herpes Simplex / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D018139:Simplexvirus / Q000276:immunology*; D014510:Urease
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D014510:Urease