Amiloride-inhibited Na+ uptake into toad bladder microsomes is Na+-H+ exchange.
Amiloride-inhibited Na+ transport into toad urinary bladder microsomes is sensitive to a pH gradient across the vesicular membrane. The magnitude of the gradient was measured directly with acridine orange. Also Na+ could stimulate amiloride-sensitive proton efflux from the microsomes. These results indicated that the transport process was Na+-H+ exchange.
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002024:Bufo marinus; D002352:Carrier Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D011719:Pyrazines / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers; D001743:Urinary Bladder / Q000378:metabolism*
D002352:Carrier Proteins; D011719:Pyrazines; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D012964:Sodium