DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites.
The genes of Herpes simplex virus type 1 (HSV-1) are classified into three temporally regulated groups. The Immediate-Early (IE) genes are transcribed first by the pre-existing transcription apparatus of the cell. The Early genes are transcribed only after IE-gene expression, and finally the Late genes are activated. The control of transcription of the HSV-1 glycoprotein D (gD) gene (an Early function) was studied by quantitative S1 mapping of RNA produced in HSV-1 infected HeLa cells after short-term transfection experiments using plasmids containing the gD promoter linked to the rabbit beta-globin gene. The viral promoter in the plasmid was activated in the same way as that in the virus itself; the RNA showed a similar time-course of appearance, dependence on prior IE-gene expression and pattern of RNA cap-sites. Deletion analysis showed that the DNA sequences necessary for Early promoter activation lie within 83 bp of the RNA cap-sites in this instance. Surprisingly, a plasmid-borne beta-globin promoter was also activated by HSV-1 infection. The mechanism of this activation, and DNA sequence similarities between the promoters of HSV-1 Early and rabbit beta-globin genes are discussed.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C039447:glycoprotein D, Human herpesvirus 1; D004262:DNA Restriction Enzymes