Molecular cloning and expression of a Bacillus subtilis beta-glucanase gene in Escherichia coli.
A Bacillus subtilis gene coding for an endo-beta-1,3-1,4-glucanase has been transferred to Escherichia coli by molecular cloning using bacteriophage lambda and plasmid vectors. The gene is contained within a 1.6-kb EcoRI-PvuI DNA fragment and directs the synthesis in E. coli of a beta-glucanase which specifically degrades barley glucan and lichenan. A novel dye-staining method has been developed to detect beta-glucanase activity in colonies on agar plates.
D001412:Bacillus subtilis / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005821:Genetic Techniques; D006026:Glycoside Hydrolases / Q000235:genetics*; D010957:Plasmids
D004262:DNA Restriction Enzymes; D006026:Glycoside Hydrolases; C028020:licheninase