Tc-99m labeled leukocytes: preparation and use in identification of abscess and tissue rejection.
A simple and reproducible method for the preparation and labeling of leukocytes with Tc-99m has been developed. Leukocytes were separated from blood, incubated with stannous pyrophosphate, and then with 20-30 mCi (740-1110 MBq) of 99mTcO-4. In leukocytes separated from human blood, the labeling efficiency was 81% +/- 6% (n = 4). Experiments on dogs with abscesses showed accumulation of the Tc-99m-labeled leukocytes in the infected sites, indicating the viability of the labeled leukocytes. Additional studies showed that rat lymphocytes that were labeled with Tc-99m, using the same technique, localized in heart transplant tissue that was being rejected.
D000038:Abscess / Q000000981:diagnostic imaging* / Q000276:immunology; D000818:Animals; D004285:Dogs; D006084:Graft Rejection; D016027:Heart Transplantation; D006801:Humans; D007553:Isotope Labeling; D007962:Leukocytes / Q000276:immunology / Q000648:ultrastructure; D008214:Lymphocytes / Q000276:immunology; D008854:Microscopy, Electron; D009206:Myocardium / Q000276:immunology; D011877:Radionuclide Imaging; D051381:Rats; D013670:Sodium Pertechnetate Tc 99m; D013667:Technetium
D013667:Technetium; D013670:Sodium Pertechnetate Tc 99m