Separation of major prostaglandins, leukotrienes, and monoHETEs by high performance liquid chromatography.
A procedure using high performance liquid chromatography (HPLC) is described for the separation of major primary cyclooxygenase metabolites (prostacyclin metabolite-6ketoPGF1 alpha, thromboxane B2, and prostaglandins F2 alpha, E2, and D2), leukotrienes (C4, B4, and D4), monohydroxyeicosatetraenoic acids (15-, 11-, 12-, and 5HETEs), and free arachidonic acid. It is therefore possible to quantitate major arachidonic acid metabolites by a single chromatographic procedure. Using this technique we have determined that a major arachidonic acid metabolite of human lung macrophages co-elutes with leukotriene B4.
D001095:Arachidonic Acids / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4 / Q000302:isolation & purification; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000032:analysis* / Q000378:metabolism; D011453:Prostaglandins / Q000302:isolation & purification*; D011460:Prostaglandins F / Q000302:isolation & purification; D013189:SRS-A / Q000302:isolation & purification*; D013929:Thromboxane B2 / Q000302:isolation & purification
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D011460:Prostaglandins F; D013189:SRS-A; D007975:Leukotriene B4; D013929:Thromboxane B2; C027356:11-hydroxy-5,8,12,14-eicosatetraenoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; C100006:prostaglandin F1