title: Calmodulin antagonists decrease the binding of epidermal growth factor to transformed, but not to normal, human fibroblasts.
abstract: Four psychoactive agents which inhibit calmodulin activity were used to study their effect on the binding of epidermal growth factor (EGF) to normal and simian-virus-40-transformed human fibroblasts (WI38). These calmodulin antagonists decreased the binding of 125I-labelled EGF to the transformed, but not to the normal, cell in a dose-dependent manner. The mechanism of this effect appears to be due to a decrease in the apparent affinity of the plasma-membrane EGF receptor for the EGF molecule.
mesh_terms: D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006220:Haloperidol / Q000494:pharmacology*; D006801:Humans; D007099:Imipramine / Q000494:pharmacology*; D013539:Simian virus 40; D014268:Trifluoperazine / Q000494:pharmacology*
chemical_list: D002147:Calmodulin; D014268:Trifluoperazine; D004815:Epidermal Growth Factor; D006220:Haloperidol; D007099:Imipramine; D002746:Chlorpromazine