Immobilization of antibody to a plastic surface by toluene 2,4-diisocyanate and its application to radioimmunoassay.
A novel procedure to fix antibody on a plastic surface was developed, using a polystyrene tube treated with 0.5% toluene 2,4-diisocyanate (TC) dissolved in carbon tetrachloride. Using 125I-labelled IgG, we found that the rate of immobilization of IgG was significantly higher than that obtained by simple adsorption or coupling by glutaraldehyde in the presence of high concentrations of IgG. The polystyrene tube coated with anti-rabbit gamma-globulin goat serum allowed a simple separation of the tracer bound to the first antibody from the free tracer in a radioimmunoassay of thyroid-stimulating hormone and prolactin, when the coating was done by the TC method but not by the simple adsorption or glutaraldehyde methods.
D000906:Antibodies; D003485:Cyanates; D005976:Glutaral; D007106:Immune Sera; D007074:Immunoglobulin G; D011126:Polypropylenes; D011137:Polystyrenes; D011388:Prolactin / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D013972:Thyrotropin / Q000032:analysis*; D014051:Toluene 2,4-Diisocyanate
D000906:Antibodies; D003485:Cyanates; D007106:Immune Sera; D007074:Immunoglobulin G; D011126:Polypropylenes; D011137:Polystyrenes; D014051:Toluene 2,4-Diisocyanate; D011388:Prolactin; D013972:Thyrotropin; D005976:Glutaral