Release of cyclic AMP and progesterone from the isolated perfused luteal ovary of the PMSG treated rat.
A model for in vitro perfusion of the isolated rat ovary was developed. In the luteinized ovaries of PMSG treated rats the relationship between perfusion flow and progesterone production was evaluated. A positive correlation was found indicating that a high blood flow rate may be necessary for an optimal secretion of progesterone from the fully developed corpus luteum in the rat. Also the effects of a continuous exposure of LH on ovarian cyclic AMP and progesterone release was studied. A rapid and sustained increase in both cyclic AMP and progesterone release was seen and this release tended to have episodic pattern with elevations occurring at intervals between 30 and 90 min following LH.
D000818:Animals; D003338:Corpus Luteum / Q000098:blood supply; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008954:Models, Biological; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000098:blood supply / Q000378:metabolism*; D010477:Perfusion; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003470:Culture Media; D006064:Gonadotropins, Equine; D012710:Serum Albumin, Bovine; D011374:Progesterone; D000242:Cyclic AMP