Isolation of a new plasmid pIRL19: its use in construction of small vectors and detection of specific sequence in a foreign DNA.
A new plasmid, pIRL19, was constructed by ligating a 1875-bp HaeII fragment carrying the ampicillin-resistance (Apr) gene to a 370-bp HaeII fragment containing the replication origin of the plasmid pBR322. The plasmid essentially contains only the basic replicator and the Apr gene. This basic replicator provides a valuable initial building block for in vitro construction of other very small vectors with antibiotic-resistance determinants. To illustrate this potential, we have transferred the chloramphenicol-resistance (Cmr) gene and a part of the Apr gene from the plasmid pBR329 into pIRL19 such that the new plasmid pIRL20 acquired the Cmr gene and maintained the integrity of its Apr structural gene.
D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis
D004247:DNA; D004262:DNA Restriction Enzymes; C031851:endodeoxyribonuclease HaeII; D015252:Deoxyribonucleases, Type II Site-Specific