A poliovirus-induced cytoplasmic membrane complex is exploited by the RNA polymerase of superinfecting Mouse Elberfeld (ME) virus.
The preexistence of a cytoplasmic membrane complex in HEp-2 cells, induced by poliovirus when inhibited in its reproduction by guanidine, was a prerequisite for accelerated reproduction of superinfecting Mouse Elberfeld (ME) virus. Guanidine-inhibited poliovirus induced a membrane complex of 470S that was successively modified into a faster sedimenting membrane complex (up to 700S) by superinfecting ME virus and exploited for ME virus reproduction. The modified membrane complex was the site for ME virus-specific RNA polymerization characterized by the existence of in vivo and in vitro activity of ME virus RNA polymerase associated with the modified membrane complex. Proof of membrane-bound RNA polymerase and newly synthesized ME virus RNA including replicative intermediate led to the conclusion that superinfecting ME virus exploits the 'poliovirus/guanidine'-induced complex as the site of action of its replication complex.
D002294:Carcinoma, Squamous Cell; D002462:Cell Membrane / Q000382:microbiology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000382:microbiology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms; D010849:Picornaviridae / Q000378:metabolism*; D017955:Poliovirus / Q000201:enzymology / Q000378:metabolism*
D012321:DNA-Directed RNA Polymerases