Solubilization and purification of Na, K-ATPase from the outer medulla of rabbit kidney.
There is great interest in the histologic localization of the Na, K-pump in various tissues: histochemical methods in this case work poorly, so a specific antibody against a purified soluble Na, K-ATPase is necessary. We approached this problem with a two-step ionic detergent treatment; the separation of the solubilized enzyme was attempted by gel filtration and ion-exchange chromatography. The gel filtration purified form was active and nearly pure, while the ion-exchange on was purer but inactive. PAGE analyses of the various enzyme forms are presented.
D000818:Animals; D002498:Centrifugation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007679:Kidney Medulla / Q000201:enzymology*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification*; D012995:Solubility
D012967:Sodium Dodecyl Sulfate; D000254:Sodium-Potassium-Exchanging ATPase