The nucleotide sequence for the large subunit of ribulose 1,5-bisphosphate carboxylase from a unicellular cyanobacterium, Synechococcus PCC6301.
The gene for the large subunit (LSU) of ribulose 1,5-bisphosphate carboxylase from a unicellular cyanobacterium, Synechococcus PCC6301, was cloned using the spinach LSU gene as a hybridization probe. The coding region of the Synechococcus LSU gene consists of 1419 nucleotides and shows 70% homology to the spinach nucleotide sequence. The derived amino acid sequence (472 amino acids) shows 81% homology to the spinach LSU and 78% to the maize LSU. Regions containing active-site residues are highly conserved among spinach, maize, and Synechococcus. In contrast, the first 13 amino acids are poorly conserved (30% homology), supporting the hypothesis that this region is proteolytically removed. The 5'-flanking region of the Synechococcus LSU gene contains sequences which correspond to bacterial consensus sequences for the -35 region and Pribnow box. Two 11-bp sequences in the 5' region show high homology to sequences in spinach and maize. One of these encompasses a possible ribosome-binding site. The 3'-flanking region contains a 35-bp sequence capable of giving rise to a terminator structure.
D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004262:DNA Restriction Enzymes; D005796:Genes; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
D004262:DNA Restriction Enzymes; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase