Comparison of effects of ethanol on platelet function and synaptic transmission.
Ethanol, at concentrations tolerated by man, is generally inhibitory to platelet function in vitro, producing significant inhibition of aggregation in response to most chemical aggregating agents. The calcium ionophore, A23187, is the agent which is most inhibited by ethanol, whereas aggregation induced by arachidonic acid is not inhibited even by concentrations of ethanol far in excess of the lethal range. This spectrum of inhibition found suggests that ethanol inhibits the platelet release reaction by a mechanism involving inhibition of Ca2+-activated phospholipase A2. These effects can be observed in superfused human platelets as well as in those suspended in buffer or in plasma. In superfused rat brain slices however, ethanol does not inhibit the A23187-induced release of radiolabelled neurotransmitter, although it can be shown to inhibit Ca2+-activated phospholipase A2 activity in rat synaptosomal preparations. It is concluded that, although there are many similarities between the effects of ethanol on the platelet and the synapse there may be differences between the way in which ethanol modifies release of intracellular contents in the two situations.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D001921:Brain / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000187:drug effects; D008297:Male; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*; D013572:Synaptic Vesicles / Q000187:drug effects; D013574:Synaptosomes / Q000187:drug effects
D007473:Ion Channels; D012701:Serotonin; D000255:Adenosine Triphosphate; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium; D004298:Dopamine