The fluorescence response of chlortetracycline-loaded human neutrophils is modulated by prostaglandin E1, but not by cyclic nucleotides.
Human neutrophils preloaded with chlortetracycline, commonly used as a probe of membrane-bound calcium, demonstrate a prompt decrease in fluorescence when exposed to surface stimuli such as the chemotactic peptide fMet-Leu-Phe. The fluorescence response was highly sensitive to preincubation with prostaglandin E1. This effect was apparently not due to elevated levels of cAMP since exogenous dibutyryl-cAMP did not alter the chlortetracycline fluorescence response to fMet-Leu-Phe. This is one of the few instances of prostaglandin E1 affecting neutrophils at physiologic concentrations, dissociated from changes in cellular cyclic nucleotide levels.
D000527:Alprostadil; D003994:Bucladesine / Q000494:pharmacology; D002751:Chlortetracycline / Q000494:pharmacology*; D000242:Cyclic AMP / Q000097:blood*; D006152:Cyclic GMP / Q000097:blood*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D011458:Prostaglandins E; D003995:Dibutyryl Cyclic GMP; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003994:Bucladesine; D000242:Cyclic AMP; D000527:Alprostadil; D006152:Cyclic GMP; D002751:Chlortetracycline