Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factor.
An increase in the level of intracellular free calcium concentration in rabbit and human neutrophils stimulated by chemotactic factors has been demonstrated directly using the calcium-sensitive fluorescent probe quin-2. Addition of f-Met-Leu-Phe (10(-9) M), C5a (3 x 10(-9) M) or leukotriene B4 (6 x 10(-8) M) to the neutrophils induces a rapid increase in the intracellular concentration of free calcium that reaches a maximum value 15 seconds following stimulation. At concentrations of f-Met-Leu-Phe less than 10(-8) M the enhancement is dose dependent with an ED50 of 8 x 10(-11) M and is significantly reduced in the presence of EGTA in the suspending medium.
D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000097:blood*; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D006801:Humans; D007975:Leukotriene B4 / Q000494:pharmacology; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000032:analysis* / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology; D011817:Rabbits
D000634:Aminoquinolines; D003182:Complement C5; D009842:Oligopeptides; D007975:Leukotriene B4; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015936:Complement C5a; C035738:Quin2-acetoxymethyl ester; D002118:Calcium