Characterization of Marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells.
Leukocyte suspensions derived from genetically Marek's disease (MD)-resistant N-line and MD-susceptible P-line chickens were fractionated at various times after exposure to the JM-10 clone of MD virus. At 3 and 5 days post exposure (DPE), during the productive-restrictive (cytolytic) phase, most infected spleen and thymus leukocytes were found to be low-density, nylon wool-adherent cells that possessed Fc receptors and surface Ia and IgM and were depleted by carbonyl iron treatment. This was true for leukocytes derived from N-line as well as those from P-line chickens. In contrast, most infected spleen cells derived from P-line chickens during the latent phase (i.e., after 7 DPE) were not found to have the above characteristics, with one exception: Ia antigen was demonstrated on the surface of latently infected cells. From these experiments it was concluded that the principal targets of the cytolytic JM-10 infection are B-cells, whereas the subsequent latent infection was found mostly in non-B-lymphocytes.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D004198:Disease Susceptibility; D008381:Herpesvirus 2, Gallid / Q000302:isolation & purification; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology* / Q000382:microbiology; D008380:Marek Disease / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis; D013997:Time Factors; D010948:Viral Plaque Assay
D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M; D011961:Receptors, Fc