title: The intron boundaries and flanking rRNA coding sequences of Calliphora erythrocephala rDNA.
abstract: We have sequenced the available cloned examples of the intron-coding sequence junctions for the rDNA of the higher Dipteran, Calliphora erythrocephala. The introns interrupt the rDNA at the same position as the type 1 intron family detected in Drosophila melanogaster and D. virilis (10,11). A duplication of 14 base pairs of the 28S rRNA coding sequence surrounds a short version of the major genomic length class of introns. This same duplication is associated with boundaries of the type 1 introns in D. virilis and D. melanogaster (10, 13,14). We have detected considerable homology between the 3' intron sequences of C. erythrocephala and D. virilis. The rRNA coding sequences flanking the introns are extremely homologous in C. erythrocephala, D. melanogaster and D. virilis, with only one small region of significant divergence. This corresponds to a variable stem region previously identified in eukaryotic 28S rRNA at a site analogous to the L1 ribosomal protein binding site of prokaryotic 23S rRNA (27).
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal; D004175:Diptera / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004247:DNA; D004262:DNA Restriction Enzymes