The detection of specific [3H]imipramine binding in bovine retina.
Using [3H]imipramine, specific imipramine binding was demonstrable in the crude membrane homogenate of bovine retina. Scatchard analysis of the saturation experiments revealed an apparent dissociation constant (Kd) of 6.4 nM and a maximal number of binding sites of 780 fmol/mg protein. These results and the substrate specificity show that the properties of imipramine binding to retinal membranes are essentially the same as described for the rat brain.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins; D002417:Cattle; D007099:Imipramine / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D002352:Carrier Proteins; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter; C027775:imipramine receptor; D007099:Imipramine