The regulation of delta-opiate receptor density on 108CC15 neuroblastoma X glioma hybrid cells.
The effect of exogenous substances on the expression of opiate receptors on 108CC15 neuroblastoma X glioma hybrid cells has been studied. Cell differentiation by culture in the presence of N6-O2-dibutyryl adenosine 3',5'-cyclic monophosphate induced a three fold increase in opiate receptor density. When the cells were grown in the presence of 10(-5) M morphine hydrochloride for up to 23 days, opiate receptor densities were reduced by only 30% when compared with matched controls. Culture in the presence of 10(-7) M D-Ala2-D-Leu5-enkephalin produced opiate receptor down regulation of 73% compared to controls after only 4 h of treatment. The down regulation process could be inhibited by continued exposure to D-Ala2 D-Leu5-enkephalin at concentrations greater than 4 nM; below this concentration down regulation was rapid and irreversible. A model to explain these observations is described.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine; D004744:Enkephalin, Methionine / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism*; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects*; D017465:Receptors, Opioid, delta
D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; D003994:Bucladesine; D009020:Morphine