A rapid filtration assay for soluble receptors using polyethylenimine-treated filters.
Ligand bound to detergent-solubilized or cytosolic receptors can be separated from free ligand by filtration through glass-fiber filters which have been pretreated with polyethylenimine (PEI). Receptors which can be assayed by this technique include detergent-solubilized muscarinic, adenosine A1, alpha 1-adrenergic, alpha 2-adrenergic, beta-adrenergic, dopamine D2, opiate, bradykinin, and benzodiazepine receptors as well as naturally soluble estradiol receptors. For muscarinic, adenosine, alpha 2, dopamine, and estradiol receptors, specific binding measured by the PEI-filter technique was 84-110% of specific binding measured by gel filtration, demonstrating that the technique gave almost quantitative recovery of bound ligand.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002850:Chromatography, Gel; D005260:Female; D005374:Filtration / Q000295:instrumentation; D006168:Guinea Pigs; D007082:Ileum / Q000378:metabolism; D008297:Male; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D011095:Polyethylenes; D051381:Rats; D017981:Receptors, Neurotransmitter / Q000302:isolation & purification*; D011987:Receptors, Steroid / Q000302:isolation & purification; D012995:Solubility; D014599:Uterus / Q000378:metabolism
D011095:Polyethylenes; D017981:Receptors, Neurotransmitter; D011987:Receptors, Steroid; D011092:Polyethylene Glycols; D011094:Polyethyleneimine