Properties and sidedness of 5'-nucleotidase in rat red cell ghosts.
The occurrence of a Mg++-activated 5'-AMPase activity in rat ghosts is demonstrated. This activity is inhibited by the alpha, beta-methyleneadenosine 5'-diphosphate, a specific inhibitor of 5'-nucleotidase. The enzyme has an apparent Km of approximately 90 microM, and is located on the exterior side of the plasma membrane.
D015720:5'-Nucleotidase; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004789:Enzyme Activation / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D009708:Nucleotidases / Q000037:antagonists & inhibitors / Q000097:blood / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D009708:Nucleotidases; D015720:5'-Nucleotidase; D008274:Magnesium