Evidence that ADP-ribosylation is not necessary for luteinizing hormone stimulation of Leydig cell steroidogenesis.
3-Aminobenzamide, which inhibits ADP-ribosylation, did not inhibit luteinizing hormone's ability to stimulate androgen secretion by mouse testicular interstitial cells in vitro. 3-Aminobenzamide, but not its inactive analog 3-amino-benzoic acid, inhibited steroidogenesis stimulated by cholera toxin.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors; D011065:Poly(ADP-ribose) Polymerases
D000728:Androgens; D001549:Benzamides; D009702:Nucleoside Diphosphate Sugars; D000246:Adenosine Diphosphate Ribose; C025160:3-aminobenzamide; D007986:Luteinizing Hormone; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases