Bile acid receptors in colorectal cancer.
Bile acids are thought to be involved in both the aetiology and development of colorectal cancer. In this study the existence of specific bile acid receptor proteins has been postulated. A receptor assay which involved labelling with 14C-deoxycholic acid was performed as well as autoradiography using 3H-deoxycholic acid. In an initial study resected colorectal cancer and adjacent histologically normal colorectal mucosa from 39 patients were studied, as were samples of normal gastric mucosa, cancers and benign colorectal tumours. Specific receptors to deoxycholic acid were detected in 12 (30.8 per cent) of the colorectal cancers, but in only 1 (2.6 per cent) of the samples from normal colorectal mucosa (X2 = 11.16, P less than 0.005). No deoxycholic acid receptors were detected in any other tissue studied. Autoradiographs of colorectal cancers showed binding of 3H-deoxycholic acid in receptor-positive tumour tissue. These findings might provide some explanation for the evidence linking bile acids with the disease.
D001345:Autoradiography; D003110:Colonic Neoplasms / Q000032:analysis*; D003840:Deoxycholic Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000032:analysis; D006801:Humans; D007417:Intestinal Polyps / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis*; D011987:Receptors, Steroid / Q000032:analysis*; D012004:Rectal Neoplasms / Q000032:analysis*; D013274:Stomach Neoplasms / Q000032:analysis
D011956:Receptors, Cell Surface; D011987:Receptors, Steroid; C038960:deoxycholic acid receptor; D003840:Deoxycholic Acid