Complete nucleotide sequence of mini-Rts1 and its copy mutant.
The nucleotide sequence of the whole mini-Rts1 genome consisting of 1,855 base pairs was determined. In addition to the cluster of five 24-base-pair direct repeats previously detected (Y. Kamio and Y. Terawaki, J. Bacteriol. 155:1185-1191, 1983), another cluster of three 21-base-pair direct repeats was found. All eight repeats were located in one direction and contained a consensus sequence TTCCCCPyPyPuPuCACACACC. Between the two clusters, a large open reading frame that could encode a 32,980-dalton polypeptide consisting of 288 amino acids was assigned. The molecular size predicted from the amino acid composition was close to the value of a unique mini-Rts1 product, the RepA protein, newly determined in minicells. A copy mutant of mini-Rts1 obtained in vitro by hydroxylamine treatment contained two-base-pair substitutions, one of which was located in the RepA protein coding region, and the other was close to the region where the oriC homologous sequences exist.
D000667:Ampicillin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D008970:Molecular Weight; D009154:Mutation; D011815:R Factors; D012091:Repetitive Sequences, Nucleic Acid; D000198:Spectinomycin / Q000494:pharmacology
D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; D000667:Ampicillin; D000198:Spectinomycin; D004262:DNA Restriction Enzymes