Developmental changes in protein carboxylmethylation and in the benzodiazepine receptor proteins in rat brain.
The intraventricular administration of [3H-methyl]-methionine resulted in a steady increase in carboxyl-[3H]methylated protein formation in the brain of developing rats (2-42 days). The analysis by gel filtration on Sepharose-4B of Lubrol-Px-solubilized membrane proteins, including benzodiazepine receptor proteins, revealed co-migration of carboxyl-[3H]methylated and [3H]flunitrazepam-binding protein species. The latter displayed a single peak at 2 days and 2-3 peaks subsequently. At 26 days the lighter of the two [3H]flunitrazepam and [3H]muscimol-binding peaks associated with the principal carboxyl-[3H]-methylated protein peak. The findings suggest the participation of carboxyl-methylation in the maturation of benzodiazepine receptor proteins.
D000375:Aging; D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism*; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005445:Flunitrazepam / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008715:Methionine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011963:Receptors, GABA-A
D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D005445:Flunitrazepam; D008715:Methionine