title: Use of plant cell cultures to study the metabolism of environmental chemicals.
abstract: The metabolism of the following environmental chemicals has been studied in cell suspension cultures of wheat (Triticum aestivum L.) and soybean (Glycine max L.):2, 4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), hexachlorobenzene, pentachlorophenol, diethylhexylphthalate , benzo [alpha] pyrene, and DDT. All chemicals tested, including the persistent ones, were partially metabolized. Polar conjugates predominated in all cases. A covalent incorporation into lignin could be demonstrated for 2,4-D and pentachlorophenol. A specific deposition in the cellular vacuole could be demonstrated for the beta-D-glucopyranoside conjugates derived from 2,4-D. A rapid assay procedure to evaluate the metabolism of a given 14C-labeled chemical in plant cell suspension cultures is described. This procedure requires about 1 week, and the reproducibility of the results obtained has been assessed.
mesh_terms: D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism; D003584:Cytological Techniques; D003634:DDT / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D006540:Herbicides / Q000633:toxicity; D006581:Hexachlorobenzene / Q000378:metabolism; D007700:Kinetics; D010416:Pentachlorophenol / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
chemical_list: D001580:Benzopyrenes; D004785:Environmental Pollutants; D006540:Herbicides; D001564:Benzo(a)pyrene; D006581:Hexachlorobenzene; D004051:Diethylhexyl Phthalate; D003634:DDT; D010416:Pentachlorophenol