Biological and macromolecular properties of murine cells persistently infected with MHV-JHM.
A persistently-infected neuroblastoma culture [Neuro-2A( JHMV )] was established with the murine hepatitis virus JHM [MHV-JHM]. After 100 days of passage, the endogenous virus [Neuro-2A( JHMV ) end] released by this culture was unable to induce the syncytia typical of MHV-JHM and the endogenous virus was not temperature-sensitive. The Neuro-2A( JHMV ) culture was cured of virus production by passage under neutralizing antibody [Neuro-2A( JHMV )Ab]. The Neuro-2A( JHMV ) and the Neuro-2A ( JHMV ) Ab cultures were as susceptible to heterologous infection with mengovirus and vesicular stomatitis virus as the uninfected Neuro-2A culture. However, the Neuro-2A ( JHMV ) and Neuro-2A( JHMV ) Ab cultures were partially resistant to homologous superinfection by MHV-JHM and the closely related MHV-A59. Virus related to MHV-JHM was rescued from the antibody-cured cells by cell fusion. The synthesis of MHV-JHM specific antigens by Neuro-2A( JHMV ) cells, Neuro-2A( JHMV ) Ab cells and 17 Cl-1 cells infected by Neuro-2A( JHMV ) end was studied by SDS-PAGE. The genomic RNAs of MHV-JHM and Neuro-2A( JHMV ) end were compared by oligonucleotide mapping. The results of the protein and RNA studies indicated that the genome of Neuro-2A( JHMV ) end was substantially modified from the genome of MHV-JHM, but the modifications did not significantly alter the molecular size of the viral-specific proteins.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005814:Genes, Viral; D051379:Mice; D006517:Murine hepatitis virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D009447:Neuroblastoma; D009500:Neutralization Tests; D009843:Oligoribonucleotides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D013045:Species Specificity; D013696:Temperature; D014762:Viral Interference; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D009843:Oligoribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins