Evidence of leukotriene B4 biosynthesis in epithelial lens cells.
Metabolism of sodium (14C)-arachidonate by bovine epithelial lens cells was studied in culture. The cells converted arachidonic acid into a major product whose formation was not inhibited by aspirin, a cyclo-oxygenase inhibitor, but was suppressed by nordihydroguaiaretic acid, a lipoxygenase inhibitor and by dexamethasone. This metabolite co-migrated with leukotriene B4 in thin layer chromatography and high pressure liquid chromatography. These data represent the first evidence for a lipoxygenase product in the lens. LTB4 could play an important role in the physiopathology of this organ.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D001095:Arachidonic Acids / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors; D004848:Epithelium / Q000378:metabolism; D007903:Lens Capsule, Crystalline / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D016859:Lipoxygenase Inhibitors; D009637:Masoprocol
D001095:Arachidonic Acids; D002396:Catechols; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D007975:Leukotriene B4; D009637:Masoprocol; D001091:Arachidonate Lipoxygenases; D001241:Aspirin