title: Shedding patterns in swine of virulent and attenuated pseudorabies virus.
abstract: Shedding patterns of 2 virulent (P-2208 and KC-152-D) and 1 attenuated (BUK) strains of pseudorabies virus (PRV) were determined in groups of intranasally inoculated feeder pigs. Clinical signs observed following inoculation with the P-2208 and KC-152-D strains included increase in rectal temperatures up to 42.2 C, anorexia, severe respiratory disturbance, and fatal CNS signs in 2 cases. Clinical signs in pigs inoculated with 7.2 X 10(7) median tissue culture infective dose (TCID50) of the BUK strain were limited to depression and a rise in rectal temperatures to 40.5 C for 3 to 4 days. Evaluation of the efficacy of the virus isolation method used showed that the presence on swabs of only 12.5 TCID50 of the P-2208 strain or 8.4 TCID50 of the BUK strain resulted in a 50% chance of virus recovery. Intranasal inoculations with 500 TCID50 of the P-2208 or KC-152-D strain did not result in synchronous infection of the whole group. Intranasal inoculations with 5,000 TCID50 of the KC-152-D strain or 50,000 TCID50 of the P-2208 strain resulted in continuous virus shedding in all pigs between postinoculation days (PID) 4 and 13 (KC-152-D strain) or 14 (P-2208 strain). Some of the pigs in these 2 groups further shed the P-2208 or KC-152-D strain in a continuous or discontinuous pattern up to PID 19 (P-2208 strain) or 20 (KC-152-D strain). The time of onset or the level of virus neutralizing antibody production in individual pigs was not found to have an influence on their shedding patterns.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D009296:Nasal Cavity / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D011557:Pseudorabies / Q000276:immunology / Q000382:microbiology*; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology*; D014774:Virulence
chemical_list: D000914:Antibodies, Viral