title: [Developing an avirulent mutant of Aujeszky's disease virus using the effect of 5 bromodesoxyuridine].
abstract: Chemicals are being used increasingly in the preparation of vaccines. Thus, an effective vaccine (MK25) against Aujeszky's Disease showing stable genetic characteristics was produced by having 5 iododesoxyuridine exert its action on a particular strain of virus. 5 Bromo-desoxyuridine also has an inhibitory effect on the synthesis of DNA viruses. The development of a new mutant of Aujeszky's Disease virus using this agent is reported. The resulting mutant, MK35, was found to be biologically stable, non-pathogenic for white mice, rabbits, sheep and piglets and, in addition, produced stable immunity in piglets, sheep and rabbits within four days after injection of this mutant.
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002353:Carrier State / Q000662:veterinary; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity*; D011557:Pseudorabies / Q000517:prevention & control*; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000302:isolation & purification / Q000627:therapeutic use*; D014774:Virulence; D014779:Virus Replication / Q000187:drug effects
chemical_list: D014765:Viral Vaccines; D001973:Bromodeoxyuridine
vernacular_title: Het ontwikkelen van een a-virulente mutant van het virus van de ziekte van Aujeszky onder invloed van de inwerking van 5 bromo-desoxy-uridine.