[3H]vasopressin binding to rat hippocampal synaptic plasma membrane. Kinetic and pharmacological characterization.
Characterization of specific vasopressin binding sites to rat hippocampal membranes has been assayed using tritiated lysine-vasopressin labelled on the tyrosyl residue. At 30 degrees C specific [3H]vasopressin binding was saturable. The estimated equilibrium dissociation constant was 7.1 nM, the mean maximal binding capacity was 78 fmol/mg protein. Arginine-vasopressin has a high affinity (Kd = 2.8 nM) and dDAVP has a low affinity (Kd = 249 nM) for hippocampal synaptic membranes. (OH)AVP and Phe2Orn8VT are at least as active as AVP in inhibiting [3H]vasopressin binding. Adenylate cyclase was activated by VIP and inhibited by PIA, but not affected by lysine-vasopressin.
D000262:Adenylyl Cyclases / Q000302:isolation & purification; D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D017483:Receptors, Vasopressin; D014667:Vasopressins / Q000378:metabolism / Q000494:pharmacology
D011956:Receptors, Cell Surface; D017981:Receptors, Neurotransmitter; D017483:Receptors, Vasopressin; D014667:Vasopressins; D000262:Adenylyl Cyclases