A batchwise purification procedure of neurofilament proteins.
A rapid batchwise purification procedure for neurofilament proteins from bovine spinal cord is described. A crude filament fraction can be obtained by treating the tissue with Triton X-100, followed by centrifugation through sucrose. From this crude filament fraction the protein is processed through a batch purification procedure using hydroxyapatite in 8 M urea. With this procedure, approximately 0.5 g of purified neurofilament protein is obtained from a single bovine spinal cord in less than 3 days.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D017886:Durapatite; D004586:Electrophoresis; D006882:Hydroxyapatites; D007381:Intermediate Filament Proteins / Q000302:isolation & purification*; D013116:Spinal Cord / Q000032:analysis*; D014508:Urea
D006882:Hydroxyapatites; D007381:Intermediate Filament Proteins; D014508:Urea; D017886:Durapatite