title: The erbB gene of avian erythroblastosis virus is a member of the src gene family.
abstract: The erbB gene of an avian erythroblastosis virus, AEV-H, was determined to be 1812 nucleotides long and was predicted to code for a protein of 67,638 daltons. Unexpectedly, a sequence of 285 amino acids in the middle of the protein showed a significant homology (38%) with the sequence in the carboxy terminus of p60src. The nucleotide sequence of a mutant of AEV-H, td-130, which induces sarcomas but not erythroblastosis in chicken, was also analyzed. A deletion of 169 nucleotides was identified in the 3' half of the erbB gene, indicating that the gene codes for a truncated protein with the predicted molecular weight of 46,667. These findings suggest that the homologous domain of erbB protein with its N-terminal portion is sufficient for the transformation of fibroblasts and that one-third of the carboxy-terminal domain has a key role for the transformation of erythroid cells.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002645:Chickens; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D011487:Protein Conformation; D001357:Sarcoma, Avian / Q000382:microbiology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins; D015688:Oncogene Protein pp60(v-src)