Regulation of proline utilization in Salmonella typhimurium: characterization of put::Mu d(Ap, lac) operon fusions.
The genes for proline utilization were fused to the structural genes of the lac operon by use of the hybrid Mu phage derivative Mu d(Ap lac). Stable deletion derivatives of these fusions were selected and used to study the transcriptional regulation of the put genes. Analysis of these fusions showed that the putA gene product, a bifunctional oxidase-dehydrogenase, also serves to negatively control transcription of the putA and putP genes. Transcription of the put genes is repressed only in putA+ strains; this repression is lifted when exogenous proline is supplied. Transcription of the put genes is stimulated by cyclic AMP in putA+ and putA strains. Maximal induction of the put genes in putA+ strains requires oxygen or an alternative electron acceptor. This oxygen effect is mediated by the putA protein since putA mutants show maximal transcription even without an electron acceptor. The orientation of the Mu d(Ap lac) insertions was determined by formation of Hfr's via the lac homology on F'ts114 lac+. The direction of chromosome mobilization by these Mu d(Ap lac)-directed Hfr's demonstrated that the putP and putA genes are divergently transcribed from a central regulatory region lying between them.
D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D026921:Amino Acid Transport Systems, Neutral; D000242:Cyclic AMP / Q000494:pharmacology; D004274:DNA, Recombinant; D004794:Enzyme Repression; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics; D009154:Mutation; D009876:Operon; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics; D010100:Oxygen / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D011394:Proline Oxidase / Q000235:genetics; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D026921:Amino Acid Transport Systems, Neutral; D004274:DNA, Recombinant; D026901:Membrane Transport Proteins; C033082:proline transporter; D011392:Proline; D000242:Cyclic AMP; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011394:Proline Oxidase; D010100:Oxygen