An improved automated periodate-resorcinol method for the determination of sialic acid.
A periodate-resorcinol method for bound sialic acid using the Technicon Autoanalyzer II is presented. It has a sensitivity similar to the manual method, is linear between 5 and 65 nmol/ml, requires less than 0.2 ml of sample, and can be run at the rate of 70 samples/h. Little cross-reaction with common matrix and cell components was found. The method is compatible with many commonly used volatile and nonvolatile chromatographic buffers. The use of a bound sialic acid standard such as N-acetylneuraminyl lactose is recommended.
D001322:Autoanalysis; D006023:Glycoproteins / Q000032:analysis; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011509:Proteoglycans / Q000032:analysis; D012118:Resorcinols; D012794:Sialic Acids / Q000032:analysis*
D006023:Glycoproteins; D011509:Proteoglycans; D012118:Resorcinols; D012794:Sialic Acids; D010504:Periodic Acid; C031389:resorcinol