title: Transfection of brown Leghorn embryo fibroblasts (BLEF) and duck embryo fibroblasts (DEF) by means of DNA isolated from RSV-transformed cells.
abstract: Transfection of Brown Leghorn embryo fibroblasts (BLEF) and embryo fibroblasts (DEF) was made by means of DNA isolated from RSV-transformed cells. DNA samples were carried out by means of phenol method, according to Svoboda at al. [16, 20] from the tumor sarcoma tissue and from cells transformed in vitro (XC and PR-RSH). DNA samples isolated from cell cultures were used for transfection BLEF and DEF cell cultures. Positive transfection results after the use DNA samples were recorded in BLEF and DEF1 cell cultures. The efficiency of transfection of BLEF is about 30% and for DEF1 10%.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D004372:Ducks; D005347:Fibroblasts; D014162:Transfection
chemical_list: D004279:DNA, Viral