Uptake by enterocytes and subsequent translocation to internal organs, eg, the thymus, of Percoll microspheres administered per os to suckling mice.
The translocation of Percoll microspheres (mean diameter 20-30 nm) from the intestinal lumen through the epithelial layer to internal organs was examined in suckling mice using transmission electron microscopy. Repeated administration of this material by gavaging for 7 consecutive days resulted in a heavy particle load of vacuolated enterocytes. A limited amount of Percoll was transported to the subepithelial tissue of both the villous mucosa and Peyer's patches where microspheres were found endocytosed, predominantly by macrophages. Even smaller numbers of particles reached mesenteric lymph nodes and, occasionally, milky spots of the omentum. Minor Percoll aggregates were easily found in Kupffer cells of the liver, indicating hematogenous translocation. Small numbers of particles were regularly detected in perivascular macrophages of the thymic cortex, which are in close contact with surrounding lymphocytes. We conclude that the thymic cortex is not totally inaccessible to particulate matter of the intestinal content.
D000818:Animals; D005260:Female; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000648:ultrastructure; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000648:ultrastructure; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008863:Microspheres; D010581:Peyer's Patches / Q000648:ultrastructure; D011205:Povidone / Q000378:metabolism*; D012822:Silicon Dioxide / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism / Q000648:ultrastructure
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone