Heterogeneity in subregions of the terminal repeats of herpes simplex virus type 2 DNA.
We constructed recombinant plasmids from the 0.5 molar terminal fragments of herpes simplex virus type 2 (HSV2) DNA. Physical mapping of three different L-terminal clones demonstrated one, two or three copies of the terminally redundant a repeat. The nucleotide sequence at the end of the terminal a repeats was shown to be identical in these three cloned HSV2 genomic L-termini and closely resembled the internal a' repeat sequence reported previously by Davison and Wilkie. Virtually all clones that were obtained from the S-termini differed in a specific subregion of the inserts. This heterogeneous region of the HSV2 genome, that varied in size from 0.2 to 0.85 M daltons, seemed to result from local deletions or amplifications and mapped near the junctions between US and c repeats.
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*
D004279:DNA, Viral