title: [Thermostability of the vaccination strain of infectious bursal disease virus].
abstract: The vaccination strain of infectious bursal disease virus, multiplied in cultures of chick embryo cells, was very resistant to heat. At a temperature of 56 degrees C the infection titre of the virus (TCID50) decreased by 0.9 log10 within two hours and by 1.2 log10 within five hours, but the virus remained infective still after 24 hours. At a temperature of 37 degrees C, a slight decrease in infection titre was recorded only after two days and a decrease by 1.2 log10 was recorded within ten days. After the 21st day the virus was almost inactivated. At a temperature of about 20 degrees C the infection titre of the virus decreased linearly from the third to the twelfth weeks. The control samples kept at +4 degrees C retained their infectivity for three months and at -20 degrees C even for six months. The discussion deals with the effect of the concentration of protein and magnesium chloride in the medium on the thermostability of infectious bursal disease virus.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D006358:Hot Temperature; D007243:Infectious bursal disease virus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D012087:Reoviridae / Q000472:pathogenicity*; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines
vernacular_title: Termostabilita vakcinacního kmene viru infekcní burzitidy.