Glycosidase analysis of large acidic-type glycopeptides from viral and cellular membrane glycoproteins. Evidence for a common oligomannosyl core with branch sugar heterogeneity.
The asparagine-linked oligosaccharides of the complex acidic-type from [3H]mannose-, [3H]glucosamine- or [3H]galactose-labelled membrane glycoproteins of BHK21 cells and Rous-sarcoma virus were analysed by gel filtration combined with extensive digestion with endo- and exo-glycosidases from bacterial and eukaryotic sources. The neutral products from the digestion with a mixture of exoglycosidases and endo-beta-N-acetylglucosaminidase D from Diplococcus pneumoniae included a series of [3H]mannose- and [3H]glucosamine-labelled neutral oligosaccharides that were all converted by digestion with eukaryotic beta-N-acetylglucosaminidases into free N-acetylglucosamine and a small oligomannosyl core containing two alpha-linked mannose residues and a third mannose residue beta-linked to N-acetylglucosamine. These studies suggested that the complex acidic-type oligosaccharides from cellular and viral membrane glycoproteins contained a common oligomannosyl core region (Man2 alpha leads to Man beta leads to GlcNAc2), with heterogeneity in the number and/or linkage of outer branch N-acetylglucosamine residues resulting in partial resistance to beta-N-acetylglucosaminidase from a bacterial source.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D006224:Cricetinae; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis*; D006023:Glycoproteins; D006026:Glycoside Hydrolases; D007668:Kidney / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008565:Membrane Proteins; D009844:Oligosaccharides / Q000032:analysis*; D014764:Viral Proteins
D006020:Glycopeptides; D006023:Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D014764:Viral Proteins; C028569:oligomannoside; D006026:Glycoside Hydrolases; D005944:Glucosamine; D008358:Mannose; D005690:Galactose