The effects of lesions in the rat hippocampus suggest the association of calcium channel blocker binding sites with specific neuronal population.
The binding of calcium antagonists in the rat hippocampal formation was studied using autoradiography. Hippocampal slices were labeled in vitro with [3H]PN 200-110. High densities of binding sites for calcium antagonists were found in the molecular layer of the dentate gyrus and in the CA3 subfield of the hippocampus. After ablation of the granule cells by local injection of colchicine a marked decrease in the number of [3H]PN 200-110 binding sites density was observed on these areas, while binding to other parts of the hippocampal formation and brain was spared. These results strongly suggest the localization of high densities of calcium channels to the granule cells of the dentate gyrus.
D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004292:Dominance, Cerebral / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002121:Calcium Channel Blockers; D002135:Calcium-Binding Proteins; D007473:Ion Channels; D002118:Calcium