Cell characterization by use of multiple genetic markers.
The extensive use of cell cultures for diverse research purposes is one of the truly great international growth industries. With the proliferation of cells comes a responsibility for monitoring them for inter- and intraspecies characteristics. We use multiple genetic markers for cell identification, i.e. species specific antigens, isozymic phenotypes, chromosomal complement, and HL-A haplotypes. The methodologies employed are briefly described, and various examples cited to show how these markers can be utilized for cell line monitoring. Data are summarized from 275 cultures sent to our laboratory for analysis during the past eighteen months. The data show that, overall, 35% of the cultures received were contaminated. The majority of cell cultures submitted were human cell lines. We found that 36% of these cultures were cross contaminated; 25% by cells of another species and 11% by another human cell line. This high incidence of inter- and intraspecies contamination underscores the importance of frequent monitoring of cell cultures.
D000818:Animals; D000941:Antigens / Q000235:genetics; D002460:Cell Line; D002472:Cell Transformation, Viral; D002875:Chromosomes / Q000502:physiology; D046508:Culture Techniques / Q000379:methods; D005796:Genes; D006680:HLA Antigens / Q000235:genetics; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007621:Karyotyping; D008297:Male; D010641:Phenotype; D013539:Simian virus 40 / Q000235:genetics
D000941:Antigens; D006680:HLA Antigens; D007527:Isoenzymes