Responsiveness of human T-lymphocyte subpopulations in autologous mixed-lymphocyte reaction using xenoprotein-free separated cells: autologous reactivity lies chiefly in a low density T-cell fraction.
The responsiveness in the autologous mixed-lymphocyte reaction (AMLR) by human T cells separated using two different methods not involving xenoantigen contract was examined. Although T cells from nylon-wool columns were active in AMLR, T cells separated by a Percoll gradient method responded poorly. Further separation of T cells from nylon-wool columns into low density (TL) and high density (TH) fractions by Percoll revealed that TL was enriched, while TH was depleted, in AMLR responsiveness. This difference could not be accounted for by differences in the helper or suppressor cells in the fractions. Moreover, TH responded well in secondary AMLR. Therefore the T cells reactive in AMLR reside chiefly, although not exclusively, in the low density fraction.
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D009757:Nylons; D011205:Povidone; D012822:Silicon Dioxide; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D009757:Nylons; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone