Isolation and characterization of an Escherichia coli mutant lacking cytochrome d terminal oxidase.
A screening procedure was devised which permitted the isolation of a cytochrome d-deficient mutant by its failure to oxidize the artificial electron donor N,N,N',N'-tetramethyl-p-phenylenediamine. Cytochrome a1 and probably cytochrome b558 were also missing in the mutant. Growth and oxygen uptake rates were similar for both parent and mutant strains. However, the strain lacking cytochrome d had an increased sensitivity to cyanide, indicating that cytochrome d confers some resistance to this respiratory inhibitor. The gene responsible for these phenotypes has been named cyd and maps between tolA and sucB.
D003486:Cyanides / Q000494:pharmacology; D017937:Cytochrome d Group; D003580:Cytochromes / Q000235:genetics* / Q000378:metabolism; D004579:Electron Transport; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D009154:Mutation; D010101:Oxygen Consumption
D003486:Cyanides; D003580:Cytochromes; D017937:Cytochrome d Group