Acetyl glyceryl ether phosphorylcholine stimulates leukotriene B4 synthesis and cyclic AMP accumulation in human polymorphonuclear leukocytes.
Both LTB4 and AGEPC stimulate PMN aggregation in a concentration-dependent manner. The lipoxygenase inhibitors ETYA and NDGA block AGEPC-induced but not LTB4-induced PMN aggregation. Agents that elevate PMN cyclic AMP levels block both LTB4-and AGEPC-induced aggregation. Paradoxically, shortly after aggregation is initiated by either AGEPC or LTB4, a transient spike in cyclic AMP levels is observed. Subsequent experiments show that LTB4 can directly elevate cyclic AMP in PMNs. Reverse-phase high-pressure liquid chromatography, coupled with selective ion gas chromatography/mass spectrometry, shows that AGEPC stimulates neutrophils to synthesize LTB4. These data suggest that AGEPC-induced neutrophil aggregation is mediated by LTB4.
D002449:Cell Aggregation / Q000187:drug effects; D000242:Cyclic AMP / Q000097:blood*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000097:blood / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*
D010972:Platelet Activating Factor; D007975:Leukotriene B4; D000242:Cyclic AMP; D007213:Indomethacin