Direct action of cyclosporin A on human B-lymphocytes with regard to immunoglobulin production.
Highly purified preparations of human B-lymphocytes were cultured with or without cyclosporin A (CyA; 1 microgram/ml) for 8 days with pokeweed mitogen (PWM) in the presence of helper factors or with Epstein-Barr virus (EBV). The amounts of IgG, IgM, IgA and IgE produced in cultures were measured by radioimmunoassays or by reverse hemolytic plaque-forming cell assays. The results demonstrate that whereas CyA had a strong suppressive effect on the production of immunoglobulins (Ig) by PWM-activated B-cells, it had an enhancing effect on EBV-activated B-cells. It is concluded that CyA has a direct effect on human B-lymphocytes and that it may suppress or enhance their activation depending on the stimulant employed to trigger the cells.
D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D003524:Cyclosporins / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology
D003524:Cyclosporins; D007136:Immunoglobulins; D011043:Pokeweed Mitogens