5'-Nucleotidase in lymphocytes from various clinical disorders.
The enzyme 5'-nucleotidase (5'-N) was demonstrated cytochemically in blood cells using a modification of the Wachstein and Meisel technique [30]. In peripheral blood the activity was found to be localised mainly in the cell membrane of lymphocytes. A semiquantitative score of 5'-N positivity, was assessed in lymphocytes from eight normal donors and 60 patients with various types of leukaemia and lymphoma. The lymphocyte score of 5'-N was slightly reduced in CML and AML but markedly reduced in most lymphoproliferative states tested. The only supranormal activity was found in two of 18 cases of CLL.
D015720:5'-Nucleotidase; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000201:enzymology; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D007943:Leukemia, Hairy Cell / Q000201:enzymology; D007945:Leukemia, Lymphoid / Q000201:enzymology; D007951:Leukemia, Myeloid / Q000201:enzymology; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology*; D009708:Nucleotidases / Q000032:analysis*
D009708:Nucleotidases; D015720:5'-Nucleotidase