High level of tyrosine protein kinase in a murine lymphoma cell line induced by Moloney leukemia virus.
Several sarcoma-inducing viruses encode protein kinases that phosphorylate tyrosine residues. Such enzymatic activities can be detected within the detergent-insoluble matrix of transformed fibroblasts. We have analysed the protein kinase activities in two murine lymphoma cell lines ( MBL2 and LSTRA) induced by Moloney murine leukemia virus (Mo-MuLV). After incubation of the detergent-insoluble matrix of these cells with [gamma-32P]ATP, several alkali-resistant phosphoproteins, including a very heavily labelled 55 000 mol. wt. protein ( p55 ), have been detected in LSTRA, reflecting the activity of a protein kinase specific to this cell line. This protein kinase activity shares some of the distinctive properties of the protein kinases of transforming viruses, i.e., specificity for tyrosine residues, association with membranous and/or cytoskeletal structures, and inhibition by a synthetic peptide derived from the phosphorylation site of pp60src. In view of the absence of a transforming gene in MoMuLV , it is likely that the high level of protein kinase detected in the LSTRA cell line arises from the expression of a cellular gene.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002472:Cell Transformation, Viral; D004790:Enzyme Induction; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008223:Lymphoma; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010750:Phosphoproteins / Q000302:isolation & purification; D011494:Protein Kinases / Q000096:biosynthesis*; D011505:Protein-Tyrosine Kinases
D000596:Amino Acids; D010750:Phosphoproteins; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases