High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli.
A cDNA clone containing the complete human alpha 1-antitrypsin sequence was isolated from a human liver cDNA bank by screening with a chemically synthesized oligonucleotide probe. DNA sequences encoding the alpha 1-antitrypsin mature polypeptide were inserted into an Escherichia coli expression vector that allows transcription from the efficient leftward promoter of bacteriophage lambda (PL) and initiation of translation at the lambda cII gene ribosome-binding site. This construction resulted in the induction of a 45-kilodalton protein at a level of approximately 15% of total cell protein. The polypeptide produced was recognized by antisera raised against human alpha 1-antitrypsin protein and displayed normal biological activity in an in vitro antielastase assay.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010957:Plasmids; D000515:alpha 1-Antitrypsin / Q000235:genetics* / Q000302:isolation & purification
D000515:alpha 1-Antitrypsin; D004247:DNA; D004262:DNA Restriction Enzymes; D010196:Pancreatic Elastase