Delimitation of a DNA sequence which confers inducibility by glucocorticoid hormones.
A chimeric long terminal repeat-thymidine kinase (LTR-tk) gene has been used to define the sequence requirements for glucocorticoid induction of gene expression. The original LTR-tk gene contains an entire mouse mammary tumor virus (MMTV) LTR preceding the tk gene. This gene can be expressed in a hormone-responsive fashion upon transfection into L tk--cells to produce a chimeric LTR-tk mRNA. Stepwise deletion of nucleotide sequences 5' of the viral RNA initiation site revealed that 202 nucleotides upstream of the viral cap site are sufficient for the hormonal regulation. Deletion of 5' sequences up to 59 nucleotides upstream of the viral cap site abolished RNA initiation in the LTR and hormonal induction.
D000818:Animals; D002678:Chimera; D004279:DNA, Viral / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005938:Glucocorticoids / Q000235:genetics*; D007739:L Cells; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D010957:Plasmids; D012315:RNA Caps / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D004279:DNA, Viral; D005938:Glucocorticoids; D012315:RNA Caps; D012367:RNA, Viral; D013937:Thymidine Kinase