Effect of prostaglandin A2 on Na+-K+-ATPase in basolateral plasma membrane of rat intestine in vitro inhibition of activation of K+-dependent p-nitrophenylphosphatase by Na+ and ATP.
1. The activation of K+-dependent p-nitrophenylphosphatase (EC 3.6.1.7) by both Na+ and ATP in rat intestinal basolateral plasma membrane is inhibited by prostaglandin A2. 2. The drug's inhibition of the activation of the enzyme by both Na+ and ATP is due to a decrease in the affinity of the enzyme for Na+ and in the Vmax of the enzyme. 3. The Ki values for this drug for Na+ and ATP in the activation of the enzyme were 45 and 70 microM, respectively.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007422:Intestines / Q000201:enzymology*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011188:Potassium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011454:Prostaglandins A / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D011454:Prostaglandins A; D000255:Adenosine Triphosphate; D012964:Sodium; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium