title: D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.
abstract: We have isolated mutants defective in high-affinity D-ribose transport. The mutations map in rbsT or rbsB , the structural gene for ribose binding protein. rbsT consists of at least one gene coding for a protein required for high-affinity transport. The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-affinity transport system for D-ribose is present in Escherichia coli K-12. rbsT and rbsB are located at min 84 on the E. coli genetic map and, together with rbsK , the gene coding for ribokinase , constitute an rbs operon. The order of genes is rbsP /O rbsT rbsB rbsK . The rbs operon is subject to negative control by the product of the rbsR gene. rbsR is located distal to the rbs operon and appears to form a separate transcriptional unit.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D033902:Periplasmic Binding Proteins; D010770:Phosphotransferases / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor); D012266:Ribose / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D033902:Periplasmic Binding Proteins; C028003:RbsB protein, E coli; D012266:Ribose; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022534:ribokinase