The cytochemical assay of NAD+ kinase activity in the follicular cells of guinea-pig thyroid gland.
A quantitative cytochemical assay for NAD+ kinase-like activity in the guinea-pig thyroid gland is described. The NADP+ produced by the activity of the kinase was used to drive the NADP+-dependent enzyme glucose-6-phosphate dehydrogenase which is endogenous to the tissue. The activity of glucose-6-phosphate dehydrogenase is greatly in excess of that of the kinase and was unaffected by the constituents of the kinase incubation medium (ATP, Mg2+ and NAD+) either alone or in combination. Kinase activity was dependent both on ATP and Mg2+, with maximal activity seen when the Mg-ATP ratio was between 1:1 and 4:1. Free ATP inhibited the activity of the enzyme. Enzyme activity was exhibited over a broad pH range (7-9) with a peak at pH 8.2. The sulphydryl-blocking agents, p-chloromercuribenzoate, iodoacetate and iodoacetamide (at 1 mM), completely abolished kinase activity but were without effect on glucose-6-phosphate dehydrogenase activity. N-ethylmaleimide and citrate (both at 1 mM) had no effect on either kinase or glucose-6-phosphate dehydrogenase activities.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006168:Guinea Pigs; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D010770:Phosphotransferases / Q000032:analysis*; D017853:Phosphotransferases (Alcohol Group Acceptor); D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology*; D013997:Time Factors
D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase