title: Isolation, purification and characteristics of mononuclear phagocytes from human placentas.
abstract: Methods for the isolation of mononuclear phagocytes from human placentas by digestion with collagenase or with trypsin are described. Mononuclear phagocytes were approximately 35% of the cells obtained. Preparations were enriched for mononuclear phagocytes by sequential density gradient centrifugation over Ficoll-Hypaque and Percoll, adherence to plastic and removal of contaminating trophoblastic cells with brief trypsin exposure. Monolayers obtained by these methods were greater than 85% mononuclear phagocytes. These cells were predominantly of fetal origin; most were mature macrophages but up to 20% appeared to be recently derived from blood monocytes as determined by ultrastructural peroxidase cytochemistry.
mesh_terms: D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006801:Humans; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology; D009000:Monocytes / Q000648:ultrastructure; D010503:Periodic Acid-Schiff Reaction; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy
chemical_list: D003470:Culture Media; D003012:Microbial Collagenase