Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose-metabolizing enzymes.
Analysis of 400 independent spontaneous mutations conferring 2-deoxygalactose resistance upon cells constitutive for the galactose pathway suggests that toxicity is due to 2-deoxygalactose-1-phosphate. Selection for and against growth on galactose in the same strain is now possible; application to systems with transcriptional or translational gene fusions to galactokinase are discussed.
D002238:Carbohydrate Epimerases / Q000235:genetics*; D004351:Drug Resistance; D005686:Galactokinase / Q000235:genetics*; D005690:Galactose / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000633:toxicity; D005796:Genes / Q000187:drug effects; D005800:Genes, Fungal / Q000187:drug effects; D005816:Genetic Complementation Test; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*; D014534:UDPglucose 4-Epimerase / Q000235:genetics*; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000235:genetics*
C019327:2-deoxy-lyxo-hexose; D005686:Galactokinase; D009713:Nucleotidyltransferases; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase; D002238:Carbohydrate Epimerases; D014534:UDPglucose 4-Epimerase; D005690:Galactose