Use of gene transfer to study expression of steroid-responsive genes.
The ability to introduce DNA into eucaryotic cells has provided a means to analyse the expression of cloned genes. By manipulating the genes in vitro using recombinant DNA techniques it is possible to identify regulatory DNA sequences which are important for individual steps in gene expression. This review will summarize how these techniques have been applied to study steroid-responsive genes and what we have learned about steroid hormone action.
D052688:Bovine papillomavirus 1 / Q000235:genetics; D002130:Calcium Phosphates / Q000494:pharmacology; D011232:Chemical Precipitation; D002678:Chimera; D003637:DEAE-Dextran / Q000494:pharmacology; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D005809:Genes, Regulator; D005822:Genetic Vectors; D010430:Pentosyltransferases / Q000235:genetics; D011523:Protoplasts; D011987:Receptors, Steroid / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D002130:Calcium Phosphates; D004274:DNA, Recombinant; D004279:DNA, Viral; D011987:Receptors, Steroid; D003637:DEAE-Dextran; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D013937:Thymidine Kinase