title: Studies on the modification of the cellular response to injury: effect of mannitol on Ehrlich ascites tumor cells following injury by anoxia or p-chloromercuribenzenesulfonic acid (PCMBS).
abstract: The possible protective effects of 30 and 180 mM mannitol added to the extracellular medium were studied in Ehrlich ascites tumor cells (EATC) injured by the nonpenetrating mercurial p-chloromercuribenzenesulfonic acid (PCMBS) or by anoxia. Protection was provided with 180 mM mannitol as observed by vital dye uptake, cell volume determinations, and electron microscopy studies in cells incubated for 1 hr in 1 mM PCMBS; however, no protection was observed after 2 and 4 hr. EATC injured by anoxia were not protected by 180 mM mannitol nor did 30 mM offer protection to cells following injury with either PCMBS or anoxia.
mesh_terms: D002731:4-Chloromercuribenzenesulfonate / Q000008:administration & dosage*; D000814:Aniline Compounds; D000818:Animals; D002021:Buffers; D002286:Carcinoma, Ehrlich Tumor / Q000139:chemically induced / Q000188:drug therapy* / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000150:complications*; D008353:Mannitol / Q000008:administration & dosage*; D051379:Mice; D010663:Phenylmercury Compounds / Q000008:administration & dosage*; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D000814:Aniline Compounds; D002021:Buffers; D010663:Phenylmercury Compounds; D008353:Mannitol; D002731:4-Chloromercuribenzenesulfonate; C002712:nigrosin