Specific binding of [3H]rauwolscine to alpha 2-adrenoceptors in rat cerebral cortex: comparison between crude and synaptosomal plasma membranes.
[3H]Rauwolscine, a specific, potent, radiolabelled alpha 2-antagonist, binds to distinct high- and low-affinity alpha 2-adrenoceptors in crude membrane preparations of the rat cerebral cortex. The concentration of high-affinity alpha 2-adrenoceptors was increased by addition of sodium ions or guanylnucleotides. In synaptosomal plasma membrane preparations, only the low-affinity component was found. Neither sodium or guanylnucleotides caused any increase in the concentration of these low-affinity receptors for [3H]rauwolscine.
D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism*; D015016:Yohimbine / Q000378:metabolism*
D011941:Receptors, Adrenergic; D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; D006165:Guanylyl Imidodiphosphate; D012965:Sodium Chloride; D006160:Guanosine Triphosphate