Evidence from binding studies for alpha 2-adrenoceptors directly associated with glomeruli from rat kidney.
Binding of the alpha 2-adrenoceptor radioligands [3H]clonidine and [3H]rauwolscine but not the alpha 1-adrenoceptor radioligand [3H]prazosin was enhanced in membranes prepared from rat isolated renal glomeruli. [3H]Rauwolscine binding to glomeruli was stereoselective with respect to the (-)-isomer of noradrenaline and the order of potency of a series of antagonists for displacement of binding indicated that the alpha 2-adrenoceptors in this preparation differ somewhat from those in some other species and tissues. Chemical sympathectomy produced no significant change in the number of sites labelled by [3H]rauwolscine indicating that few of the alpha 2-adrenoceptors in glomerular membranes are associated with sympathetic nerve terminals.
D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000032:analysis; D007678:Kidney Glomerulus / Q000032:analysis*; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000032:analysis*; D011942:Receptors, Adrenergic, alpha / Q000032:analysis* / Q000378:metabolism
D011941:Receptors, Adrenergic; D011942:Receptors, Adrenergic, alpha