Effect of chronic ethanol treatment and withdrawal on mitochondrial redox activity in brain cortical slices.
The effect of chronic ethanol treatment on cytochrome a-a3 redox activity was studied in rat brain cortical slices. The slices were depolarized by electrical pulses and the level of reduced cytochrome a-a3 was directly measured within the slice by dual wavelength spectrometry. Cortical slices cut from the brains of rats withdrawn from ethanol demonstrated less reduction of cytochrome a-a3 following electrical depolarization compared both to controls and ethanol addicted rats. It is suggested that this effect of ethanol withdrawal on cytochrome a-a3 redox activity is due to a change in the regulation of mitochondrial activity.
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D017936:Cytochrome a Group; D003580:Cytochromes / Q000378:metabolism; D004558:Electric Stimulation; D004579:Electron Transport / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
D017936:Cytochrome a Group; D003580:Cytochromes