title: Protection of soluble benzodiazepine receptors from heat inactivation by GABAergic ligands.
abstract: Benzodiazepine receptors were solubilized from calf brain cortex by the ionic detergent deoxycholate and by the nonionic detergent Triton X-100. Approximately 90% of the soluble benzodiazepine receptors of both preparations were heat inactivated within 30 min at 55 degrees C. 100 microM of gamma-aminobutyric acid (GABA) protected 80% of Triton X-100 solubilized benzodiazepine receptors and 56% of the deoxycholate soluble benzodiazepine receptors from heat inactivation. Time course of heat inactivation showed that the deoxycholate soluble receptors are more sensitive to heat than the Triton X-100 soluble receptors.
mesh_terms: D000818:Animals; D002417:Cattle; D003840:Deoxycholic Acid; D004355:Drug Stability; D005445:Flunitrazepam / Q000378:metabolism; D006358:Hot Temperature; D007093:Imidazoles / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A; D012995:Solubility; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D007093:Imidazoles; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam; D017830:Octoxynol; C005954:imidazoleacetic acid