[Laboratory procedures in adenoviruses. VIII. Immunoglobulin class-specific ELISA for the demonstration of antibodies against adenovirus hexon].
Serum pairs from 114 patients with adenovirus infections (mostly ocular disease in adults) were investigated by an ELISA, with purified adenovirus 5 hexon coated to the solid phase, diluted sera, and peroxydase-labeled antihuman IgG, IgA, and IgM antibodies. Serum dilutions of 1:3200, 1:200, and 1:100 respectively proved to be optimal to demonstrate antibody rises. Sera from infants served as negative controls. Antibody rises were found in the IgG and/or IgA test 85% of the patients, but only in 56% by complement fixation. Antibody rises in all three ELISA tests occurred mostly around the 10th day after onset of disease. Fewer antibody rises and weak reactions were found in IgM test. Moreover, IgM antibodies were present also in persons without fresh adenovirus infection. Hence, this test is not suitable for an early diagnosis. This observation is probably due to the frequent stimulation of the immune system by the genusspecific determinant alpha of the hexon by repeated adenovirus infections during life.
D000257:Adenoviridae Infections / Q000276:immunology*; D000258:Adenovirus Infections, Human / Q000276:immunology*; D000260:Adenoviruses, Human / Q000276:immunology*; D000914:Antibodies, Viral / Q000032:analysis*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D014764:Viral Proteins; C018315:hexon capsid protein, Adenovirus
Laboratoriumspraxis bei Adenoviren. VIII. Immunglobulinklassen-spezifischer ELISA zum Nachweis von Antikörpern gegen Adenovirus-Hexon.