An improved method for the solubilization of stable gonadotropin receptors.
The studies presented herein describe the usefulness of glycerol in enhancing the binding activity and stability of detergent-solubilized gonadotropin (LH/hCG) receptors. The data presented show that when Leydig tumor cells (designated MA-10) are extracted with Triton X-100 in the presence of glycerol, one can quantitatively solubilize the cellular LH/hCG receptors without losing hormone-binding activity. It is also shown that in the presence of glycerol, the free soluble receptors can be maintained for at least 3 days with little or no reduction in their hormone-binding activity. These findings should prove useful for future studies on the structure and properties of gonadotropin receptors.
D000818:Animals; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004355:Drug Stability; D005990:Glycerol; D007457:Iodine Radioisotopes; D007984:Leydig Cell Tumor / Q000032:analysis*; D008722:Methods; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D011974:Receptors, LH; D012995:Solubility
D006063:Chorionic Gonadotropin; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D005990:Glycerol