Localization of hepatitis A virus antigen to specific subcellular fractions of hepatitis-A-infected chimpanzee liver cells.
The localization of hepatitis A virus antigen to specific subcellular fractions of infected chimpanzee liver cells was studied by solid-phase radioimmunoassay following a mild subcellular fractionation procedure designed to separate various organelles from the cytosol and the nuclei. Most of the antigenic activity (93%) was evenly divided between the cytosol fraction and the microsomal suspension. Within the microsomal fraction, more than 75% of the detectable antigen was associated with the smooth endoplasmic reticulum. Less than 4% of the total antigenic activity was localized to the nucleus. These data provide additional evidence that replication of hepatitis A virus occurs primarily within the cytoplasm of the host cell in close association with cellular membranes, consistent with that observed for other members of the genus Enterovirus.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D003600:Cytosol / Q000276:immunology; D004721:Endoplasmic Reticulum / Q000276:immunology; D006506:Hepatitis A / Q000276:immunology*; D006507:Hepatovirus / Q000276:immunology* / Q000502:physiology; D008099:Liver / Q000032:analysis*; D008861:Microsomes / Q000276:immunology; D002679:Pan troglodytes / Q000382:microbiology; D014779:Virus Replication
D000956:Antigens, Viral