Strain differences in brain alpha2- and beta-adrenergic receptor binding in dystrophic mice.
We have examined alpha 2-adrenergic [( 3H]-clonidine) and beta-adrenergic [( 3H]-dihydroalprenolol; DHA) binding in brain membranes from four different dystrophic mouse mutants: 129/ReJ-dy; 129 B6F1/J-dy; C57BL/6J-dy and C57BL/6J-dy2J. As shown previously, male 129/ReJ-dy mice have significantly fewer brain [3H]-DHA binding sites than their littermate controls. In marked contrast, the remaining three mutants showed no differences in [3H]-DHA binding when compared to their respective controls although there were inter-strain differences, e.g., C57BL/6J-dy2J mice have significantly fewer binding sites than the 129B6F1/J-dy mice. On the other hand, we saw no strain differences in alpha 2-binding. In one mutant, however, (129 B6F1/J-dy) we observed a 50% increase in alpha 2-binding sites compared to littermate controls. We conclude that weight loss or undernutrition may be an important factor in determining brain levels of adrenergic binding sites in dystrophic mouse brain.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D003000:Clonidine / Q000378:metabolism; D004082:Dihydroalprenolol / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013045:Species Specificity
D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D003000:Clonidine