The stimulation of Na+, K+-ATPase activity in the medulla of the rat kidney by [arginine] vasopressin and its analogues.
Total and Na+, K+-ATPase activities have been measured in sections (10 micron thick) from blocks of rat kidney cultured for 5 h at 37 degrees C, pH 7.5, in Glasgow Eagle's Minimum Essential Medium. Synthetic [arginine]vasopressin [( Arg]VP) stimulated ATPase activity in the thick tubular cells of the renal outer medulla over the concentration range 0.01-10 fmol/l, but failed to affect ATPase activity in the proximal and distal convoluted tubules of the cortex. The increase in medullary total ATPase activity induced by [Arg]VP and its analogues was wholly due to stimulation of Na+, K+-ATPase activity. Stimulation of medullary ATPase activity was blocked by [Arg]VP antiserum. The [Arg]VP analogues desmopressin, [lysine]vasopressin, [arginine]vasotocin and oxytocin stimulated medullary Na+, K+-ATPase activity, the three last-named analogues being considerably less potent than [Arg]VP.
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D046508:Culture Techniques; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D005260:Female; D006651:Histocytochemistry; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology*; D007686:Kidney Tubules, Distal / Q000187:drug effects / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology; D008236:Lypressin / Q000494:pharmacology; D010121:Oxytocin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014668:Vasotocin / Q000494:pharmacology
D001127:Arginine Vasopressin; D010121:Oxytocin; D008236:Lypressin; D000254:Sodium-Potassium-Exchanging ATPase; D003894:Deamino Arginine Vasopressin; D014668:Vasotocin