title: Mechanisms of beta-lactam resistance in British isolates of Pseudomonas aeruginosa.
abstract: The mechanisms of resistance to beta-lactam antibiotics in 191 isolates of Pseudomonas aeruginosa were examined. These represented the most resistant organisms of 1866 isolates collected during a national survey of antibiotic resistance in this species. One hundred and seventy-two isolates were selected because they were resistant to carbenicillin (MIC greater than 128 mg/L) and 19 because the MICs of cefotaxime were greater than the MICs of carbenicillin. Of the carbenicillin-resistant isolates, 35 produced plasmid-mediated beta-lactamases known to be active against carbenicillin and seven produced unusual beta-lactamases; in 131 strains, resistance could not be attributed to beta-lactamase production and was considered to be intrinsic. The unusual antibiogram in which the MIC of cefotaxime was greater than the MIC of carbenicillin was associated with overproduction of the chromosomally-determined Sabath and Abrahams ' beta-lactamase. Selection of strains with this last mechanism represents a novel resistance problem and one which may increase with increased use of newer antipseudomonal beta-lactams.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001390:Azlocillin; D002228:Carbenicillin / Q000494:pharmacology*; D002438:Cefoperazone / Q000494:pharmacology; D002439:Cefotaxime / Q000494:pharmacology*; D002442:Ceftazidime; D002511:Cephalosporins / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D007526:Isoelectric Point; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006113:United Kingdom; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D010406:Penicillins; D002438:Cefoperazone; D002442:Ceftazidime; D001618:beta-Lactamases; D002228:Carbenicillin; D001390:Azlocillin; D002439:Cefotaxime