title: [Establishment of a human ependymoma cell line].
abstract: We established a cell line derived from a cerebellar ependymoma and named it as NU 2-26. NU 2-26 cells grew well in vitro with Eagle's MEM plus L-15 medium (6% FCS) alone. Flat epithelial cells grew like a sheet in groups in monolayer culture. Chromosome analysis revealed near diploid and mode number was 46. Colony forming efficiency in soft agar was 51% and 36%, inoculated 1 X 10(2) cells and 1 X 10(3) cells in 60 mm dish, respectively. NU 2-26 cells formed solid tumor differed from the original ependymoma. There were more spindle shaped cells and pseudorosettes were uncommon. Dibutyryl adenosine 3'; 5'-cyclic monophosphate (db-cAMP) and cytochalasin B induced NU 2-26 cells astrocytic transformation in vitro. These changes were reversible, but became irreversible when NU 2-26 cells were treated with each drugs over seven days long. It suggested that NU 2-26 cells have a glial character.
mesh_terms: D000293:Adolescent; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D002528:Cerebellar Neoplasms; D003571:Cytochalasin B / Q000494:pharmacology; D004806:Ependymoma; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
chemical_list: D003571:Cytochalasin B; D003994:Bucladesine