Inhibition of concanavalin A-induced phosphatidylinositol turnover and lysosomal enzyme release by cyclic AMP-elevating agents in rat peritoneal macrophages.
When rat peritoneal macrophages were stimulated with concanavalin A (Con A), phosphatidylinositol (PI) turnover was rapidly induced and concomitantly N-acetylglucosaminidase was released. These two reactions were markedly inhibited by prostaglandin E1, which elevated the cellular cyclic AMp levels. This effect was mimicked by dibutyryl cyclic AMP. These results suggest that the elevation of the cellular cyclic AMP levels might play a role in the inhibition of the early stages of Con A-induced activation of macrophages through the inhibition of PI turnover.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000527:Alprostadil; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D003994:Bucladesine / Q000494:pharmacology; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D010716:Phosphatidylinositols; D011458:Prostaglandins E; D003208:Concanavalin A; D003994:Bucladesine; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase; D000118:Acetylglucosaminidase; D000527:Alprostadil