Comparison of the viral proteins of canine parvovirus-2, mink enteritis virus and feline panleukopenia virus.
Canine parvovirus-2 (CPV-2), Mink enteritis virus (MEV) and feline panleukopenia virus (FPV) were produced using identical cell culture and purification techniques. The distributions of the haemagglutinating activity of the three different parvoviruses in a CsCl gradient were similar with haemagglutinating peaks identified at 1.48-1.49, 1.42, 1.36 and 1.30-1.31 g cm-3. The number and distribution of the viral proteins and the equivalent protein molecular weights are similar for all three viruses in SDS-polyacrylamide gels (10%). Four viral proteins were identified and their molecular weights were determined: protein A (77 500-79 500), protein B (63 000-63 500), protein C (61 500-63 000) and protein D (50 000-55 000). The viral protein D although reported for some other parvoviruses has not previously been demonstrated in CPV-2, MEV or FPV.
D000818:Animals; D002415:Cats; D002500:Centrifugation, Isopycnic; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005254:Feline Panleukopenia / Q000382:microbiology; D028301:Feline Panleukopenia Virus / Q000032:analysis*; D008907:Mink / Q000382:microbiology; D008970:Molecular Weight; D010321:Parvoviridae / Q000032:analysis*; D010322:Parvoviridae Infections / Q000382:microbiology / Q000662:veterinary; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins