Energy deprivation of Zajdela hepatoma cells upon in vivo treatment with thiamphenicol.
In vivo inhibition of mitochondrial protein synthesis in Zajdela hepatoma by thiamphenicol accompanied by about 70% decrease in mitochondrial cytochrome oxidase activity resulted in a marked inhibition of the tumor growth. Under similar conditions liver regeneration was not appreciably affected. Although the glycolytic capacity of Zajdela hepatoma cells increased after in vivo thiamphenicol treatment, the cellular contents and ratios of adenine nucleotides indicated energy deprivation of the treated cells.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D006019:Glycolysis; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013839:Thiamphenicol / Q000627:therapeutic use*
D000227:Adenine Nucleotides; D003576:Electron Transport Complex IV; D013839:Thiamphenicol