Chromatin organization and restriction endonuclease activity on human metaphase chromosomes.
Human metaphase chromosomes were treated with HaeIII, HindIII, EcoRI, and AluI restriction endonucleases and subsequently stained with either Giemsa or ethidium bromide. The results obtained seemed to suggest that the structural organization of specific chromosome regions can play a primary role in determining the cytological effect after digestion with specific restriction endonucleases.
D001399:Azure Stains; D002843:Chromatin / Q000032:analysis*; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004996:Ethidium; D006570:Heterochromatin / Q000032:analysis; D006801:Humans; D008677:Metaphase
D001399:Azure Stains; D002843:Chromatin; D006570:Heterochromatin; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases; D004996:Ethidium