Gel-permeation high-performance liquid chromatography as a powerful technique for rapid analysis of calcitriol (1,25-dihydroxycholecalciferol) receptors.
We used gel-permeation high-performance liquid chromatography to detect cytosolic and nuclear calcitriol (1,25-dihydroxycholecalciferol) receptors. This new convenient technique allowed accurate separation of the calcitriol receptor from other vitamin D-binding proteins. Compared with sucrose-density-gradient centrifugation, it has several advantages including, in particular, its rapidity.
D000818:Animals; D002117:Calcitriol / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000032:analysis; D004386:Duodenum / Q000032:analysis; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000032:analysis*
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol