Characterization of the Aleutian disease virus genome and its intracellular forms.
Aleutian disease virus (ADV) of mink is a nondefective parvovirus with a single-stranded DNA genome. We characterized the viral DNA forms found in infected cells prepared by a modified Hirt extraction procedure. Double-stranded DNA molecules corresponding in size to 4.8-kilobase-pair duplex monomers and 9.6-kilobase-pair duplex dimers were identified in agarose gels by blot hybridization to 32P-labeled ADV DNA. A rapidly reannealing ADV duplex monomer was isolated on a preparative scale and physically mapped with a series of restriction endonucleases. The map derived was similar to one derived from double-stranded ADV DNA produced by self-primed synthesis on virion DNA, but differed from restriction endonuclease maps reported for other parvovirus DNAs. The purified duplex monomer could be labeled with [32P]dCTP by nick translation and used as a probe in blot hybridization to detect ADV sequences in DNA from small numbers of infected cells. Additional studies indicated that double-stranded ADV DNA could first be detected at 24 h after infection.
D000454:Aleutian Mink Disease Virus / Q000235:genetics*; D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D007668:Kidney; D007700:Kinetics; D014771:Virion / Q000032:analysis; D014776:Virus Cultivation; D014779:Virus Replication; D014713:Viruses, Unclassified / Q000235:genetics*
D004279:DNA, Viral