Enzyme-linked immunosorbent assay for the detection of human rotavirus in stools.
A simple method for the detection of human rotavirus in stools is described, using a double antibody sandwich enzyme-linked immunosorbent assay. Polysterene microtitre plates were used as solid phase. Four capture antibodies were tried, bovine, egg-derived, guinea pig and monoclonal antibody to rotavirus. Both bovine and egg-derived antirotavirus labelled with horseradish peroxidase were used as the detecting antibodies. The results obtained were compared with a commercially available ELISA, Rotazyme (Abbott Laboratories), and also with the direct detection of rotavirus by electron microscopy. Bovine antibody was found to be an unsuitable capture antibody due to non-specific false positive reactions.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005243:Feces / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D012401:Rotavirus / Q000276:immunology* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000175:diagnosis*
D000914:Antibodies, Viral; D000956:Antigens, Viral