title: A human B-cell line (BES-1) forming rosettes with sheep erythrocytes, producing Forssman antibody, and phagocytizing sheep erythrocytes.
abstract: A lymphoid cell line, designated BES-1, belonging to "lymphoblastoid cell lines with B-cell properties (B-LCL)" and derived from some normal B lymphocytes, was established in an attempt at primary culture of peripheral blood from a patient with acute myelogenous leukemia (AML). BES-1 cells unexpectedly formed rosettes with sheep erythrocytes (SRBC), which are usually a T-cell marker, and phagocytized SRBC. Subsequent study indicated that BES-1 cells produce antibody specific to Forssman (F) antigen, but not to Paul-Bunnell (P-B) or Hanganutzin-Deicher (H-D) antigen.
mesh_terms: D000328:Adult; D000818:Animals; D000917:Antibody Formation; D015478:Antigens, Heterophile / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004912:Erythrocytes; D005260:Female; D005577:Forssman Antigen / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007621:Karyotyping; D010587:Phagocytosis; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology
chemical_list: D015478:Antigens, Heterophile; D000956:Antigens, Viral; D007075:Immunoglobulin M; D005577:Forssman Antigen