Histamine, 5-hydroxytryptamine and compound 48/80 activate PMN-leucocyte collagenase of the rat.
Experimental pleurisy in rat and intraperitoneal injection of histamine, serotonin and the histamine releaser compound 48/80 caused changes in blood leucocyte populations. An increased number of PMN-leucocytes was observed. The peripheral blood PMN-leucocytes of non-treated rats contained collagenase mainly in the latent form. During experimental pleurisy and after treatment with histamine, serotonin and compound 48/80 activation of latent collagenase of up to 90-98% was obtained. Mepyramine, cimetidine and timegadine inhibited activation of latent collagenase by compound 48/80. It is suggested that histamine is involved in the process of activation of leucocyte collagenase in the course of inflammation.
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology*; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D003012:Microbial Collagenase / Q000097:blood*; D009504:Neutrophils / Q000201:enzymology*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D003012:Microbial Collagenase