Iron uptake system medicated by Vibrio anguillarum plasmid pJM1.
Plasmid pJM1 from an invasive strain of Vibrio anguillarum mediates an iron-sequestering system that is associated with the ability of this bacterium to cause septicemia in marine fishes. This plasmid-mediated iron uptake system was analyzed by using mutations caused by transposon Tnl. Restriction endonuclease analysis of iron uptake-deficient and -proficient derivatives generated by insertion of Tnl and molecular cloning experiments permitted us to localize the plasmid regions involved in the process of iron sequestration to a stretch of about 20 kilobase pairs. In addition, the existence of two plasmid-mediated components involved in the process of iron uptake in V. anguillarum was defined: a diffusible substance which functions as a siderophore and a nondiffusible receptor for complexes of iron-siderophore, which we have tentatively identified as the pJM1 plasmid-mediated outer membrane protein OM2 of V. anguillarum.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007501:Iron / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D010957:Plasmids; D013045:Species Specificity; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism
D008565:Membrane Proteins; D007501:Iron; D004262:DNA Restriction Enzymes