Mineralocorticoid receptors in the epithelial cells of human colon and ileum.
Specific binding of [3H]aldosterone to the cytosolic fraction of epithelial cells was studied in the human colon and terminal ileum. Analysis of [3H]aldosterone binding to the epithelial cells of ascending colon, caecum and ileum as a function of [3H]aldosterone concentration revealed only one class of specific receptors with an affinity constant of about 2 nmol/l. [3H]aldosterone binding was approximately the same in the sigmoid, descending and transverse colon and in the caecum, but slightly lower in the ascending colon and ileum. The specificity of the [3H]aldosterone binding was the same along the colon. The relative order of potency in inhibiting [3H]aldosterone binding was: aldosterone = SC 26304 = dexamethasone much greater than dihydrotestosterone greater than estradiol = RU 26988.
D000450:Aldosterone / Q000378:metabolism; D001667:Binding, Competitive; D003080:Cold Temperature; D003106:Colon / Q000166:cytology*; D003907:Dexamethasone / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007082:Ileum / Q000166:cytology*; D007700:Kinetics; D008297:Male; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid / Q000378:metabolism*; D013148:Spironolactone / Q000031:analogs & derivatives / Q000378:metabolism
D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid; D013196:Dihydrotestosterone; D013148:Spironolactone; C006286:SC 26304; D000450:Aldosterone; D004958:Estradiol; D003907:Dexamethasone