Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody.
We used hemadsorption immunosorbent technique (HIT) to detect mumps immunoglobulin M (IgM) antibody. IgM from human sera was adsorbed into anti-human IgM-coated wells in plates, and mumps-specific IgM was detected by adding mumps virus hemagglutinin and guinea pig erythrocytes consecutively. Specific IgM-positive sera showed hemadsorption, whereas negative sera showed hemagglutination. All 81 patients with current mumps infections tested showed mumps-specific IgM antibody, with titers ranging from 160 to 327,680. In most cases IgM antibody was already present on day 1 or 2 after the onset of illness. IgM antibody persisted for 6 to 10 weeks. Of 57 patients with acute respiratory illnesses caused by parainfluenza virus, 5 showed cross-reactions in the hemadsorption immunosorbent technique assay for mumps IgM. The hemadsorption immunosorbent technique assay is specific for the IgM class of antibody and avoids false-positive results due to rheumatoid factor. This test is an efficient and sensitive method for rapid and early diagnosis of mumps infections.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D005189:False Positive Reactions; D006168:Guinea Pigs; D006382:Hemadsorption; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007163:Immunosorbent Techniques; D007223:Infant; D009107:Mumps / Q000175:diagnosis / Q000276:immunology*; D018108:Respirovirus / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M