Is acetylation of protein involved in membrane function?
[3H]-acetate is rapidly incorporated as the acetyl moiety into synaptosomal protein and the apparent rate appears to decrease after approximately 1-2 minutes. A second dose of labeled acetate given 6 minutes after the first shows the same time dependent process suggesting that the protein substrate is not depleted. The apparent fall-off in the rate may represent the approach to a steady state of the mixing of the added acetate with internal cold acetate. Veratridine or batrachotoxin appears to stimulate a deacetylation process and tetrodotoxin blocks the effect of veratridine. Several proteins are acetylated at least one of which appears to be a glycoprotein of relatively low molecular weight. The presence of cold pyruvate or glucose competes with the incorporation of labeled acetate; the implication is that glucose and pyruvate can serve as a source of acetyl CoA for protein acetylation. The studies suggest that acetylation-deacetylation processes may be involved in membrane function, possibly in ion and/or transmitter channels.
D000085:Acetates / Q000378:metabolism*; D000107:Acetylation; D000818:Animals; D001496:Batrachotoxins / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000494:pharmacology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects; D013574:Synaptosomes / Q000378:metabolism; D014701:Veratridine / Q000494:pharmacology
D000085:Acetates; D001496:Batrachotoxins; D006023:Glycoproteins; D007656:Ketoglutaric Acids; D009419:Nerve Tissue Proteins; D011773:Pyruvates; D003208:Concanavalin A; D014701:Veratridine; D019289:Pyruvic Acid; D009439:Neuraminidase