Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7.
We present the complete sequence of an mRNA which is induced by estrogen in the human breast cancer cell line MCF-7 [pS2 mRNA, Masiakowski et al., Nucleic Acids Res. 10, 7895-7903 (1982)]. Primer extension and cloning of double-stranded cDNA (ds-cDNA) into a vector designed to make full-length cDNA were used to determine the sequence of the fifteen 5'-terminal nucleotides which were not present in the original pS2 ds-cDNA clone. The mRNA sequence has a major open reading frame encoding 84 amino-acids, flanked by a 40 nucleotide 5'-untranslated region and a 198 nucleotide 3'-untranslated region preceding the polyA tail. The 3'-untranslated region contains a polyadenylation signal, AUUAAA, 14 nucleotides upstream from the polyA tail. The derived protein sequence contains a putative signal peptide region suggesting that the protein may be secreted. The nucleotide and derived amino-acid sequences were compared to previously determined sequences, particularly to those of hormone-regulated proteins and growth factors, and no obvious similarities were observed.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005796:Genes / Q000187:drug effects*; D005822:Genetic Vectors; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*
D003062:Codon; D004967:Estrogens; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes