Phosphodiester bonds between polypeptides and chromosomal DNA.
Polypeptides co-purifying with DNA in alkali are covalently bound to DNA. DNA purified by treatment with alkali, sodium dodecyl sulphate and phenol absorbed 125I under conditions designed to radioiodinate exclusively tyrosine and histidine in peptides. A significant amount of the absorbed 125I remained associated with DNA during treatment with phenol as well as during precipitation with ethanol from neutral and alkaline solutions. However, after prolonged digestion with proteinase K, most of the radiolabelled material could be removed from 125I-treated DNA. Further treatment with a second protease (Pronase) released no larger fraction of the 125I label. The residual radiolabelled material could be precipitated together with DNA by ethanol and it remained associated with DNA also in the presence of alkali (95 degrees C), acid (37 degrees C) and hydroxylamine (37 degrees C). In contrast, radiolabelled peptides were released from DNA by treatment with hot piperidine (10% at 95 degrees C) and by agents that hydrolyse peptides and modify DNA, e.g. strong acid (95 degrees C) and formic acid/diphenylamine. The radiolabelled peptides, once released from DNA by these chemical methods, could be further cleaved by Pronase. This shows that the residual DNA/peptide complex isolated after prolonged protease digestion is protease-resistant unless it is cleaved or otherwise modified by harsh chemical treatment. The linking groups between deoxynucleotides and the radiolabelled residual peptides could be isolated by digestion of DNA in the DNA/peptide complex. Radiolabelled peptides could be released from this linking group material by phosphodiesterases, indicating the involvement of phosphodiesters in the linking groups.
D000143:Acids; D000468:Alkalies; D000596:Amino Acids; D002855:Chromatography, Thin Layer; D004247:DNA / Q000302:isolation & purification*; D019286:Endopeptidase K; D010450:Endopeptidases; D007457:Iodine Radioisotopes; D010755:Organophosphates; D010455:Peptides / Q000302:isolation & purification*; D010727:Phosphoric Diester Hydrolases; D010880:Piperidines; D011402:Pronase
D000143:Acids; D000468:Alkalies; D000596:Amino Acids; D007457:Iodine Radioisotopes; D010755:Organophosphates; D010455:Peptides; D010880:Piperidines; D004247:DNA; D010727:Phosphoric Diester Hydrolases; D010450:Endopeptidases; D019286:Endopeptidase K; D011402:Pronase