Immunological properties of lymphoblastoid cell lines (UHKT) - 1, 2, 3, 4, 5, 6) established from cases of human leukemias.
Immunological characteristics of stabilized cell lines (UHKT-1, 2, 3, 4, 5, 6) established from cells of peripheral blood of patients with leukemia were studied. Attention was focussed on the analysis of various membrane markers (e. g. T, Ia-like, antigens, membrane immunoglobulin, receptors for sheep erythrocytes, for IgG-Fc fragment, for the third component of the complement and expression of beta-2 microglobulin). The cells of these lines were also tested for the presence of Epstein-Barr virus-determined nuclear antigen (EBNA) and other EBV antigens. Chromosomal analysis was performed repeatedly. The UHKT cell lines were compared to the standard cell lines obtained from other laboratories. The results showed that rabbit antiB xenosera revealed Ia- antigen on the cells of B cell lines. The determination of T and B cell types using rosette test was less convincing in some lines.
D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D004854:Herpesvirus 4, Human / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012397:Rosette Formation
D000954:Antigens, Surface; D000956:Antigens, Viral; D000949:Histocompatibility Antigens Class II; D011947:Receptors, Antigen, B-Cell