Antioxidant effect of diethyldithiocarbamate on microsomal lipid peroxidation assessed by low-level chemiluminescence and alkane production.
Different thiol-containing compounds, such as diethyldithiocarbamate (DDC), glutathione, penicillamine, and dithioerythritol have been chosen to study their effect on ascorbate/Fe-ADP-induced lipid peroxidation, detected by low-level chemiluminescence and alkane production. In the concentration range used, these thiols exerted a temporary protection against lipid peroxidation by lengthening the induction period; after overcoming this induction period, no substantial inhibition of either chemiluminescence or alkane production was observed. DDC was effective in protecting against lipid peroxidation in the nanomolar range, whereas the group of other thiol-containing molecules operated in the millimolar range.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000473:Alkanes / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D049449:Luminescence; D008862:Microsomes, Liver / Q000187:drug effects*; D051381:Rats; D013859:Thiocarbamates / Q000494:pharmacology*
D000473:Alkanes; D008054:Lipid Peroxides; D013859:Thiocarbamates; D000244:Adenosine Diphosphate; D004050:Ditiocarb; D007501:Iron; D001205:Ascorbic Acid