Amikacin resistance mediated by multiresistance transposon Tn2424.
Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, which conferred higher levels of resistance in Pseudomonas aeruginosa than in Escherichia coli. A restriction analysis and cloning experiments resulted in a physical and functional map of Tn2424. Comparison by a heteroduplex technique revealed that Tn2424 includes the total sequence of Tn21 and two additional DNA fragments that are 1.8 and 4 kilobases long.
D000583:Amikacin / Q000494:pharmacology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D007612:Kanamycin / Q000031:analogs & derivatives*; D008854:Microscopy, Electron; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D007612:Kanamycin; D000583:Amikacin; D004262:DNA Restriction Enzymes