Comparison of two methods of genetic exchange in determination of the genetic locus of the structural gene for Pseudomonas aeruginosa elastase.
A mutation, lasA1, that results in the synthesis of enzymatically inactive elastase protein was mapped by R68.45 plasmid-mediated conjugation and transposon-facilitated recombination to ca. 75 min on the Pseudomonas aeruginosa PAO chromosome. Since immunologically cross-reactive material is produced, the mutation presumably arose within the elastase structural gene.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D010196:Pancreatic Elastase / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D010196:Pancreatic Elastase