Mapping of binding sites for Mu repressor and ner product within the left-end EcoRI. C fragment of the Mu genome.
Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are at least 3 distinct binding sites for the Mu repressor and 1 binding site for the ner protein on the EcoRI. C fragment. The possible biological function of these binding sites is discussed.
D010583:Bacteriophage mu / Q000235:genetics*; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D004279:DNA, Viral; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI