Extraction of SV40 T-antigen from lysates of transformed cells.
Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca2+ at low temperature, conditions that also cause a specific depolymerization of microtubules.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000276:immunology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D007908:Lens, Crystalline / Q000502:physiology; D008970:Molecular Weight; D013539:Simian virus 40 / Q000276:immunology*; D013347:Subcellular Fractions / Q000276:immunology
D000957:Antigens, Viral, Tumor