title: Evidence for mucins and sialic acid as receptors for Pseudomonas aeruginosa in the lower respiratory tract.
abstract: The nature of the receptors for mucoid and nonmucoid Pseudomonas aeruginosa was investigated by using adherence to injured tracheal epithelium as a model. Bovine submaxillary mucin and crude rat tracheal mucin inhibited the adherence of both types of P. aeruginosa. Among the sugars present in these mucins only N-aceylneuraminic acid inhibited adherence. Inhibition of adherence probably involved the binding of N-acetylneuraminic acid to the bacterial cells and not to the tracheal cells. The mucoid strain appeared to be much more sensitive to inhibition by N-acetylneuraminic acid. Periodate oxidation and cholera filtrate also reduced the adherence of both strains, but Clostridium perfringens neuraminidase treatment did not alter adherence. A nonmucoid isogenic mutant of an unstable mucoid strain was also inhibited by N-acetylneuraminic acid. These data suggest that the receptor for P. aeruginosa is a sialic acid moiety on cell surfaces or in mucins.
mesh_terms: D000268:Adhesiveness; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D004848:Epithelium / Q000382:microbiology; D051379:Mice; D009077:Mucins / Q000502:physiology*; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000502:physiology*; D012794:Sialic Acids / Q000502:physiology*; D014132:Trachea / Q000382:microbiology*
chemical_list: D002241:Carbohydrates; D009077:Mucins; D012794:Sialic Acids; D010504:Periodic Acid; D009439:Neuraminidase