Single amino acid substitution in 30K protein of TMV defective in virus transport function.
Involvement of the tobacco mosaic virus (TMV) coded 30K protein in a virus transport function within the infected plant has been suggested. Previously a temperature sensitive mutant, TMV Ls 1, that is defective in cell-to-cell movement at a restrictive temperature, was reported. To demonstrate a relationship between the 30K protein and the transport function, the nucleotide sequences of the 30K and coat protein cistrons of the mutant, TMV Ls 1, and the wild type, TMV L (tomato strain) were compared. A single base substitution which causes replacement of a proline codon in the L strain by a serine codon was found in the Ls 1 mutant. Results support the notion that the 30K protein is responsible for the virus transport function.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D009154:Mutation; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism
D000596:Amino Acids; D004247:DNA; D004262:DNA Restriction Enzymes