Sustained oscillations in a reconstituted enzyme system containing phosphofructokinase and fructose 1,6-bisphosphatase.
In a reconstituted open and homogeneous enzyme system containing phosphofructokinase, fructose 1,6-bisphosphatase, pyruvate kinase, adenylate kinase, and glucose-6-phosphate isomerase sustained oscillations could experimentally be generated. The approach is based on a stirred flow-through reaction chamber. The periodic motions of the reactants are mainly caused by the antagonistic allosteric effects of the adenine nucleotides on the activities of the phosphofructokinase and fructose 1,6-bisphosphatase.
D000494:Allosteric Regulation; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D006019:Glycolysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008956:Models, Chemical; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013552:Swine
D010732:Phosphofructokinase-1; D006597:Fructose-Bisphosphatase