Kinetics of expression of infectious ecotropic, xenotropic, and mink cell focus-forming murine leukemia virus after 5-iododeoxyuridine induction of cells from high- and low-leukemia mouse strains.
Endogenous murine leukemia virus (MuLV) was induced with 5-iododeoxyuridine (IdUrd) from the high-leukemia mouse strain AKR and from two low-leukemia strains, C3H/He and BALB/c. A virus-free cell line from strain AKR readily gave rise to infectious, XC-positive MuLV upon treatment with IdUrd, whereas cells from strains C3H/He and BALB/c produced replication-deficient, XC-negative MuLV. IdUrd-induced cells also produced xenotropic and mink cell focus-forming MuLV. Xenotropic virus emerged at a higher titer from both AKR and BALB/c cells during two discrete time intervals, first at day 3 after induction and a second time during spread of the induced ecotropic MuLV. Xenotropic and mink cell focus-forming MuLVs were also produced by IdUrd-induced C3H/He cells but required another round of infection in Sc-1 cells for detection. The in vitro infectivity of endogenous ecotropic MuLV isolated by IdUrd induction from C3H/He cells correlated with pathogenicity in the Fv-1-compatible, leukemia-negative mouse strain NFS/N. Thus, the virulence of endogenous ecotropic MuLV may be an important factor in determining the leukemia incidence in these inbred strains of mice.
D000406:AKR murine leukemia virus / Q000254:growth & development; D000818:Animals; D002460:Cell Line; D007065:Idoxuridine / Q000494:pharmacology; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D051379:Mice; D008806:Mice, Inbred AKR / Q000382:microbiology; D008807:Mice, Inbred BALB C / Q000382:microbiology; D008809:Mice, Inbred C3H / Q000382:microbiology; D008815:Mice, Inbred Strains / Q000382:microbiology*; D014775:Virus Activation
D007065:Idoxuridine