title: Relationship of cyclic-AMP levels in leukotriene B4-stimulated leukocytes to lysosomal enzyme release and the generation of superoxide anions.
abstract: Leukotriene B4 stimulated the formation of cyclic AMP, the release of lysosomal enzyme and generation of superoxide anions by human leukocytes. Dose-response curves have shown that the enzyme release proceeded in parallel with increments in cyclic AMP, suggesting a linkage between cyclic AMP and leukotriene B4-induced leukocyte activation. However, preincubation of the cells with (5S,12S)-dihydroxy-6,8,10,14-eicosatetraenoic acid or leukotriene B4 resulted in a dose-dependent inhibition of leukotriene B4-induced degranulation, without causing parallel changes in the levels of cyclic AMP. Both dihydroxy acids also blocked leukotriene B4-induced superoxide anion generation. These results suggest that the leukocyte responses to leukotriene B4 and the concomitant cyclic-AMP increments may be merely coincidental. In addition, the present study further supports the suggestion that (5S,12S)-dihydroxy-6,8,10,14-eicosatetraenoic acid may modulate the action of leukotriene B4 in the leukocyte.
mesh_terms: D001095:Arachidonic Acids / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D007975:Leukotriene B4 / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology; D013481:Superoxides / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D013481:Superoxides; D007975:Leukotriene B4; C034922:5,15-dihydroxy-6,8,11,13-eicosatetraenoic acid; D000242:Cyclic AMP