Lack of correlation between thymidine kinase activity and the antiviral or antiproliferative response to interferon.
Murine L cells lacking thymidine kinase activity (Ltk-) do not respond to the antiviral or antiproliferative activity of interferon, whereas human tk- cells show a normal response. Furthermore, a clone of tk+ cells derived from Ltk- cells by DNA-mediated transfer of the tk herpes gene does not respond to interferon. Cells of this clone and Ltk- cells do not produce interferon when infected with Newcastle disease virus or treated with poly(I) X poly(C) and DEAE-dextran. Possible reasons for this inability to produce interferon and the lack of response to interferon are discussed.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003637:DEAE-Dextran / Q000494:pharmacology; D004686:Encephalomyocarditis virus / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000494:pharmacology*; D007700:Kinetics; D007739:L Cells / Q000201:enzymology; D051379:Mice; D009522:Newcastle disease virus / Q000235:genetics*; D011070:Poly I-C / Q000494:pharmacology; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
D007370:Interferon Type I; D003637:DEAE-Dextran; D013937:Thymidine Kinase; D011070:Poly I-C