title: Chromate resistance plasmid in Pseudomonas fluorescens.
abstract: Chromate resistance of Pseudomonas fluorescens LB300, isolated from chromium-contaminated sediment in the upper Hudson River, was found to be plasmid specified. Loss of the plasmid (pLHB1) by spontaneous segregation or mitomycin C curing resulted in a simultaneous loss of chromate resistance. Subsequent transformation of such strains with purified pLHB1 plasmid DNA resulted in a simultaneous re-acquisition of the chromate resistance phenotype and the plasmid. When pLHB1 was transferred by conjugation to Escherichia coli, the plasmid still conferred chromate resistance.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002840:Chromates / Q000494:pharmacology*; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D008670:Metals / Q000494:pharmacology; D009154:Mutation; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000235:genetics*; D011815:R Factors
chemical_list: D000900:Anti-Bacterial Agents; D002840:Chromates; D004251:DNA Transposable Elements; D008670:Metals