Specific high-affinity receptors for 1,25-dihydroxyvitamin D3 in human peripheral blood mononuclear cells: presence in monocytes and induction in T lymphocytes following activation.
Human peripheral blood monocytes have high affinity binding sites for 1,25-(OH)2D3 (Kd 0.14 nM, sedimentation coefficient 3.7S). Resting human peripheral blood T lymphocytes, however, do not have a demonstrable 1,25-(OH)2D3 receptor. After activation with phytohemagglutinin the T cells exhibit the receptor within 24 h, and this expression is blocked by cycloheximide. The receptor in activated T lymphocytes has a sedimentation coefficient of 3.7S and a high affinity (Kd 0.10 nM) for the ligand.
D002117:Calcitriol / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D009000:Monocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D010835:Phytohemagglutinins; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol