title: A detailed physical and genetic map of two R.ColBM IncFIII plasmids.
abstract: The cleavage and genetic maps of two closely related R.ColBM IncFIII plasmids, designated pSAS1201 and pSAS1203, are presented. Restriction analysis of both plasmids with SstI, EcoRI, Bg/II, XhoI, HindIII, and Sa/I indicated that the maps of these two plasmids are superimposable with the exception of a 1.70-MDa DNA segment absent in pSAS1203. Our results provide the first physical and genetic maps of plasmids belonging to the IncFIII incompatibility group.
mesh_terms: D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D012093:Replicon
chemical_list: D004262:DNA Restriction Enzymes