Physostigmine prolongs the elementary event underlying decay of inhibitory postsynaptic currents in Aplysia.
Inhibitory postsynaptic currents (IPSCs) recorded at cholinergic synapses between identified neurons of the buccal ganglia of Aplysia decay exponentially. IPSC decay has been ascribed to a receptor-channel-dependent kinetic process, rather than to changes in subsynaptic acetylcholine (ACh) concentration, but is prolonged by the anticholinesterase eserine. Our data are consistent with a direct effect of 1.6 X 10(-4) M eserine on an elementary channel process. IPSC decay remains single exponential during eserine prolongation, suggesting that eserine slows the kinetic process responsible for decay, rather than substituting an additional kinetic process. In either control sea water or eserine, noise spectra computed from currents induced by pressure ejection of ACh are fitted by the sum of two Lorentzians. The slow corner frequency f1 is reduced from 6.3 to 3.5 Hz by eserine, consistent with a direct eserine action on receptor-channel kinetics, whereas apparent single-channel conductance was unchanged. Both IPSCs and ACh noise recorded in the same cells were comparably slowed by eserine. In eserine, decay time constant tau agreed with noise f1; however, tau and f1 in control sea water differed by 31%. The discrepancy may be accounted for by invoking an additional component to the recorded noise spectra, perhaps produced by synaptically active choline. In addition to the direct effect on kinetics, prolonged exposure to eserine produces a slow extra component to the IPSC decay tail.
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D001048:Aplysia / Q000502:physiology*; D005071:Evoked Potentials / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects; D007700:Kinetics; D009474:Neurons / Q000502:physiology*; D010830:Physostigmine / Q000494:pharmacology*; D011950:Receptors, Cholinergic / Q000502:physiology*
D007473:Ion Channels; D011950:Receptors, Cholinergic; D010830:Physostigmine; D000109:Acetylcholine