[Measurements of non-electrogenic fluxes through lipid bilayers induced by Men+/nH+-exchangers].
In the presence of concentration gradient of metal ions on bilayer lipid membrane (BLM) the addition of non-electrogenic carriers results in a formation of concentration gradient of hydrogen ions in the unstirred layers near membrane. Addition of protonophore under these conditions brings about the formation of diffusion potential of hydrogen ions. This effect underlies the method of measuring non-electrogenic fluxes on BLM initiated in the presence of Men+/nH+ - exchangers. The proposed method was tested on the following Men+/nH+ - exchangers: nigericin, monensin and A23187. The order of cationic selectivity of the given carriers obtained by measuring the potentials on BLM in the presence of protonophores agrees with literature data, which were obtained by direct measurements of ionic fluxes.
D002411:Cation Exchange Resins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007473:Ion Channels / Q000187:drug effects*; D007475:Ion Exchange Resins / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D008051:Lipid Bilayers; D008564:Membrane Potentials / Q000187:drug effects; D008985:Monensin / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology
D002411:Cation Exchange Resins; D007473:Ion Channels; D007475:Ion Exchange Resins; D007476:Ionophores; D008051:Lipid Bilayers; D008985:Monensin; D009550:Nigericin
Izmereniia neélektrogennykh potokov cherez bisloĭnye lipidnye membrany, indutsiruemye Men+/nH+-obmennikami.