Isolation of surface membranes from neuronal and non-neuronal cell populations from embryonic chick brain. Opiate receptor activity of neuronal membranes.
Surface membranes were isolated from neuronal and non-neuronal cell populations prepared from embryonic chick brain. Sodium dodecyl sulfate gel electrophoresis showed that membranes from the two cell populations have distinctly different protein compositions. The neuronal cell membranes have opiate receptor activity with high- and low-affinity binding sites similar to those determined for intact neuronal cells.
D000818:Animals; D001921:Brain / Q000166:cytology*; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D009270:Naloxone / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011957:Receptors, Opioid / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D011957:Receptors, Opioid; D009270:Naloxone; D000254:Sodium-Potassium-Exchanging ATPase