title: Complete nucleotide sequence of alfalfa mosaic virus RNA 2.
abstract: Double-stranded cDNA of in vitro polyadenylated alfalfa mosaic virus (AlMV) RNA 2 has been cloned and sequenced. The use of an oligodeoxyribonucleotide corresponding to the known sequence of the 5'-end of RNA 2 to prime second-strand DNA synthesis, enabled us to construct the complete primary structure of AlMV RNA 2. The sequence of 2,593 nucleotides contains a long open reading frame for a protein of Mr 89,753 starting at the first AUG codon from the 5'-end. This coding region is flanked by a 5'-terminal sequence of 54 nucleotides and a 3'-noncoding region of 166 nucleotides which includes the sequence of 145 nucleotides the three genomic RNAs of AlMV have in common.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D000455:Medicago sativa; D008970:Molecular Weight; D009029:Mosaic Viruses / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D003062:Codon; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA; D004262:DNA Restriction Enzymes