Ultrastructural localization of 5'-nucleotidase in guinea pig neutrophils based upon the use of cerium as capturing agent.
A cytochemical method for 5'-nucleotidase localization in which cerium serves as the capture agent in order to enzymatically detect liberated inorganic phosphate has been developed. The method has been established in cell-free model systems and in guinea pig neutrophils where 5'-nucleotidase is restricted to the plasmalemma as an ectoenzyme. This cerium-based method gives better results for ultrastructural localization of 5'-nucleotidase than conventional lead-based methods.
D015720:5'-Nucleotidase; D000249:Adenosine Monophosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002563:Cerium / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods*; D008297:Male; D008954:Models, Biological; D009504:Neutrophils / Q000201:enzymology / Q000648:ultrastructure*; D009596:Nitrophenols / Q000378:metabolism; D009708:Nucleotidases / Q000097:blood*; D009943:Organophosphorus Compounds / Q000378:metabolism
D005994:Glycerophosphates; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D002563:Cerium; C008644:nitrophenylphosphate; D000249:Adenosine Monophosphate; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; C031463:beta-glycerophosphoric acid