Cyclic AMP-mediated potentiation of muscarinic hyperpolarization in neuroblastoma cells.
Adenosine, 2-chloroadenosine and prostaglandin E1 which are known to increase cyclic AMP in neuroblastoma cells potentiated the acetylcholine-induced muscarinic hyperpolarization of the cells without changing the resting membrane potential. The potentiation caused by 2-chloroadenosine was further augmented by Ro 20-1724, a phosphodiesterase inhibitor. A direct intracellular pressure application of cyclic AMP potentiated the muscarinic hyperpolarization without changing the resting membrane potential. Morphine which inhibits adenylate cyclase antagonized 2-chloroadenosine-induced potentiation of the muscarinic hyperpolarization. These results suggest that changes in cyclic AMP level modulate the muscarinic response of neuroblastoma cells.
D015762:2-Chloroadenosine; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000527:Alprostadil; D000818:Animals; D000242:Cyclic AMP / Q000502:physiology*; D004298:Dopamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007478:Iontophoresis; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009116:Muscarine / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology
D011458:Prostaglandins E; D015762:2-Chloroadenosine; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; D009020:Morphine; D009116:Muscarine; D000242:Cyclic AMP; D000527:Alprostadil; D000241:Adenosine; D000109:Acetylcholine; D004298:Dopamine