Adenovirus 2 early gene expression promotes susceptibility to effector cell lysis of hybrids formed between hamster cells transformed by adenovirus 2 and simian virus 40.
Weakly oncogenic adenovirus 2 (Ad2)-transformed LSH hamster cells are sensitive to lysis by spontaneously cytolytic lymphoid cells and activated macrophages, whereas highly oncogenic simian virus 40 (SV40)-transformed LSH cells are relatively resistant to these nonspecific effector cells. Somatic cell hybrids formed between Ad2- and SV40-transformed hamster cells, which expressed Ad2 tumor (T) antigens, exhibited an increased cytolytic susceptibility compared to Ad2 T antigen-negative cell hybrids or nonhybrid SV40-transformed cells. No correlation was found between the expression of SV40 T antigen in hybrid cells and cytolytic susceptibility. The results suggest the existence of a novel function for early Ad2 genome-encoded polypeptides (T antigens) expressed in transformed hamster cells--the induction of susceptibility to destruction mediated by immunologically nonspecific effector cells.
D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003602:Cytotoxicity, Immunologic; D004198:Disease Susceptibility; D004622:Embryo, Mammalian; D005814:Genes, Viral; D008264:Macrophages / Q000276:immunology*; D008647:Mesocricetus; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral