Specific binding of guanosine 5'-diphosphate with the NS protein of vesicular stomatitis virus.
A soluble protein fraction containing L, NS, G and M proteins of vesicular stomatitis virus was prepared by treatment of Triton-disrupted virions with 0.8M NaCl. Incubation of the soluble fraction with beta-32P GDP followed by analysis of the proteins by polyacrylamide gel electrophoresis showed specific labeling of the NS protein. The NS-GDP complex was sensitive to phosphatase treatment, suggesting non-covalent binding. No binding of GDP to NS protein was detected when the soluble fraction was pre-heated at 100 degrees C for 1 min. or Mg++ was omitted from the incubation mixture. The binding was inhibited by ATP consistent with competition for a common nucleotide binding site.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006150:Guanine Nucleotides / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D007668:Kidney; D010761:Phosphorus Radioisotopes; D011485:Protein Binding; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
D006150:Guanine Nucleotides; D010761:Phosphorus Radioisotopes; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D006153:Guanosine Diphosphate