Synthesis and biological activities of neurokinin alpha and beta.
Two novel neuropeptides, neurokinin alpha and beta isolated from porcine spinal cord, were announced. We have synthesized neurokinin alpha as Asp-Met-His-Asp-Phe-Phe-Val-Gly-Leu-Met-NH2, which were 98-99% pure by HPLC. Assays on the isolated guinea pig ilium showed neurokinin alpha to have 81% and neurokinin beta to have 65% of the activity of Substance P. Knowledge of these three related peptides having a common activity opens new considerations of their intrinsic physiological roles in neurotransmission versus pharmacological activities, and reappraisal of the diverse activities of Substance P including that in the inflammatory response of the eye.
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D006168:Guinea Pigs; D007085:Ilium / Q000187:drug effects; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins / Q000138:chemical synthesis* / Q000494:pharmacology; D015288:Neurokinin A; D015287:Neurokinin B; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013552:Swine
D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; D015288:Neurokinin A; D015287:Neurokinin B