Monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus.
Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, highly specific, and reproducible assay for the detection of bovine enteric coronavirus was developed, using a mixture of two of these monoclonal antibodies reactive with antigenic components either external or internal to the virion. These monoclonal antibodies were bound indirectly to 96-well plates via rabbit anti-mouse immunoglobulin. After sample application and incubation, virus was detected by using rabbit anti-coronavirus peroxidase conjugate followed by enzyme substrate and chromagen. Fecal samples from a single herd of cows were screened for the presence of coronavirus by this assay. Five percent of clinically normal cows were found to be shedding coronavirus.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003332:Coronaviridae / Q000276:immunology / Q000302:isolation & purification*; D003333:Coronaviridae Infections / Q000382:microbiology / Q000662:veterinary*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D007124:Immunoenzyme Techniques; D011817:Rabbits
D000911:Antibodies, Monoclonal