Proton NMR measurements of hydrogen exchange at the C-3 position of 3-hydroxybutyrate in suspensions of rat liver mitochondria.
Rat liver mitochondria suspended in buffer made with 2H2O catalyse exchange C-3 proton of added D-3-hydroxybutyrate with solvent deuterons. The kinetics of this process can be followed using spin-echo proton NMR. The observed isotope exchange velocity is sensitive to the activity of D-3-hydroxybutyrate dehydrogenase, which is an integral protein of the inner mitochondrial membrane. A method is described which can be used to obtain the specific isotope exchange velocity of the enzyme in the intact mitochondrion.
D020155:3-Hydroxybutyric Acid; D000818:Animals; D002244:Carbon; D006859:Hydrogen / Q000378:metabolism; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008930:Mitochondria, Liver / Q000378:metabolism*; D011522:Protons; D051381:Rats
D006885:Hydroxybutyrates; D011522:Protons; D002244:Carbon; D006859:Hydrogen; D006884:Hydroxybutyrate Dehydrogenase; D020155:3-Hydroxybutyric Acid