Structure elucidation by one- and two-dimensional 360- and 500-MHz 1H NMR of the oligosaccharide units of two glycoproteins isolated from alveoli of patients with alveolar proteinosis.
The structure of the oligosaccharide units of the glycoproteins of Mr 36,000 and 62,000 isolated from alveoli of patients with alveolar proteinosis have been determined by one- and two-dimensional 1H NMR spectroscopy at 500 and 360 MHz. Bi-, tri-, and tetraantennary glycans of N-acetyllactosaminic type have been found in high percentage. They are 1----6 monofucosylated and fully sialylated, the ratio NeuAc(2----3)/NeuAc X (2----6) increasing with increasing degree of branching.
D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification*; D011522:Protons; D011649:Pulmonary Alveolar Proteinosis / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000032:analysis*
D006023:Glycoproteins; D009844:Oligosaccharides; D011522:Protons