title: Determination of the sequence alteration in the DNA of the herpes simplex virus type 1 temperature-sensitive mutant ts K.
abstract: The alteration in the DNA sequence responsible for the mutant phenotype of the herpes simplex virus type 1 temperature-sensitive mutant ts K has been determined. A single C:G base pair present in the wild-type Vmw 175 immediate-early polypeptide-coding sequence has been replaced by T:A in the mutant gene. This results in the substitution of an alanine by a valine codon. In two revertants the mutant T:A base pair has been resubstituted by C:G.
mesh_terms: D001483:Base Sequence; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000378:metabolism*; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D018139:Simplexvirus / Q000235:genetics*; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins