Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor.
Active DNA helicase I (Mr 180,000) can be isolated from Escherichia coli F+ strains but not F- strains. The transfer of the F sex factor to F- strains by conjugation permits the purification of the enzyme from the transconjugant strains. We conclude from this that helicase I is coded for by a portion of the F factor. Results also obtained by using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggest that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor.
D001482:Base Composition; D004265:DNA Helicases / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005144:F Factor; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D010957:Plasmids
D029968:Escherichia coli Proteins; D004262:DNA Restriction Enzymes; C027093:TraI protein, E coli; D004265:DNA Helicases