Binding of propranolol and iodocyanopindolol to isolated cells, homogenates and plasma membranes of rat liver, lung, kidney and heart.
3H-(+/-) propranolol and 125I-(+/-) cyanopindolol have been used to characterize beta adrenoceptors of liver, lung, kidney and heart of rat. Two main binding parameters, KD and Bmax were measured using either cells, homogenates or plasma membranes of each organ (except heart). Results show that the most accurate determination of KD and Bmax involves: (1) a previous extraction of plasma membranes (2) the use of a ligand of a high affinity for beta adrenoceptors (3) a high specific radioactivity of this ligand. 125I-(+/-) cyanopindolol seems to be a better ligand than 3H(+/-) propranolol for such determinations.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D020120:Iodocyanopindolol; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010869:Pindolol / Q000031:analogs & derivatives* / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism
D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D011433:Propranolol; D010869:Pindolol