Effect of streptococcal extracellular nuclease on the carrier activity of RNA for streptolysin S.
Upon digestion with a streptococcal extracellular nuclease, yeast RNA yielded acid-insoluble core having increased carrier activity for streptolysin S. The carrier activity was found in minor fractions of the core which were eluted from a DEAE-cellulose column at higher salt concentrations. Upon gel filtration through a Sephadex G-75 column, the effective component (Fr. I) was eluted earlier than bulk oligonucleotides (Fr. II). Nucleotide composition (in mol %) of Fr. I was AMP: 21.8; GMP: 55.1; CMP: 8.2; UMP: 14.9, whereas that of Fr. II was AMP: 38.0; GMP: 33.1; CMP: 8.0; UMP: 20.9. Chromatographic patterns of SLS complex induced by Fr. I were similar to those of the toxin formed in the presence of active fraction prepared from RNase I core. Hemolytic activity of the latter complex was, like the former, unaffected by streptococcal nuclease treatment. The carrier activity of DNA digested with the nuclease was also investigated.
D000818:Animals; D001426:Bacterial Proteins; D002417:Cattle; D004247:DNA; D004720:Endonucleases / Q000378:metabolism*; D007700:Kinetics; D012331:RNA, Fungal; D012441:Saccharomyces cerevisiae; D013291:Streptococcus / Q000201:enzymology*; D013301:Streptolysins; D013950:Thymus Gland
D001426:Bacterial Proteins; D012331:RNA, Fungal; D013301:Streptolysins; C026050:streptolysin S; D004247:DNA; D004720:Endonucleases; C037681:streptococcal nuclease