Gene amplification in the lac region of E. coli.
We have characterized strains of E. coli in which the lac region, together with varying amounts of surrounding DNA, is amplified 40 to 200 fold. The amplification events involve regions of 7 to 37 kb and result in a tandem array of repeated units. Restriction digest patterns of DNA from over 100 independent strains reveal that the amplified units are different in each case. Mechanisms of gene duplication and amplification, and the relationship of gene amplification in bacteria to that in eucaryotic cells, are considered.
D001483:Base Sequence; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005784:Gene Amplification; D007763:Lac Operon; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes