Delineation of the catalytic components of the NADPH-dependent O2- generating oxidoreductase of human neutrophils.
Four catalytic components of the NADPH-dependent O2- generating oxidoreductase of human neutrophils have been identified. DCIP reductase, cytochrome c reductase and a chromophore 450-455 reductase are present in phorbol myristate acetate stimulated neutrophils and absent in resting cells and phorbol myristate acetate stimulated chronic granulomatous disease cells. Quinol dehydrogenase activity has also been demonstrated in activated and resting cells. Furthermore, a chromophore absorbing in the reduced state at 450-455 nm participates in superoxide production. This chromophore is reduced by NADPH or duroquinol and is missing in cell lysates derived from a patient with chronic granulomatous disease.
D003579:Cytochrome Reductases / Q000097:blood; D006105:Granulomatous Disease, Chronic / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000097:blood* / Q000302:isolation & purification; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D011808:Quinone Reductases / Q000097:blood; D012016:Reference Values; D013481:Superoxides / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013481:Superoxides; D009247:NADH, NADPH Oxidoreductases; D003579:Cytochrome Reductases; D019255:NADPH Oxidases; D011808:Quinone Reductases; C033633:dichlorophenolindophenol reductase; C022709:superoxide-forming enzyme; D013755:Tetradecanoylphorbol Acetate