An amplification system using BSA-antibody conjugate for sensitive enzyme immunoassay.
A procedure is described for sensitive titration of antibodies, macromolecular antigens and haptens by enzyme immunoassay. It involves using first antigen or antibody labelled with bovine serum albumin (BSA) and then an anti-BSA antibody conjugated with an enzyme. The performance characteristics of this assay are indicated and compared with those for conventional enzyme immunoassay. The present procedure allowed fast sensitive titration of human IgE, rabbit type III anti-streptococcal antibody and cAMP.
D000907:Antibodies, Bacterial / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D005976:Glutaral; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis; D012710:Serum Albumin, Bovine; D001616:beta-Galactosidase
D000907:Antibodies, Bacterial; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D000242:Cyclic AMP; D001616:beta-Galactosidase; D005976:Glutaral