Rapid detection of rotavirus in stool by latex agglutination: comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test.
A latex agglutination test (LX) using antisera prepared against Nebraska calf diarrhea virus (NCDV) is described for the detection of rotavirus in stool of children with acute gastroenteritis. The test was compared with electron microscopy (EM) and radioimmunoassay (RIA) with 100 stools positive or negative for rotavirus. Out of 53 stools positive in RIA or EM, 49 were positive in LX and 4 were negative. Two specimens negative in EM and RIA were falsely positive in LX. The method was also tested in two clinical series with 115 stools from 101 children. Altogether 67/115 stools were positive in RIA, and 62/115 in LX. Out of 7 stools with contradictory results, 6 were negative in LX but positive in RIA, and 1 was positive in LX but negative in RIA. The results indicate that the LX is suitable for rapid screening of rotavirus gastroenteritis in clinical practice.
D000956:Antigens, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005243:Feces / Q000276:immunology*; D006801:Humans; D007841:Latex Fixation Tests; D008854:Microscopy, Electron; D011863:Radioimmunoassay; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis*
D000956:Antigens, Viral