Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VIII/RAg in nervous tissue after fixation.
The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.
D000941:Antigens / Q000032:analysis*; D001932:Brain Neoplasms / Q000032:analysis / Q000648:ultrastructure; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D005353:Fibronectins / Q000032:analysis*; D005404:Fixatives; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis*; D003012:Microbial Collagenase; D009417:Nerve Tissue / Q000032:analysis* / Q000648:ultrastructure; D010434:Pepsin A; D014357:Trypsin; D014841:von Willebrand Factor
D000941:Antigens; D005353:Fibronectins; D005404:Fixatives; D007797:Laminin; D014841:von Willebrand Factor; D005169:Factor VIII; D014357:Trypsin; D010434:Pepsin A; D003012:Microbial Collagenase