Incorporation in liposomes as a method for the application of genotoxins of low water solubility in the SCE assay.
Benzo[alpha]pyrene BaP), freely dissolved or incorporated in liposomes prepared from egg yolk lecithin, was checked for SCE induction in Chinese hamster V79-E and rat liver RL-19 cells in vitro. SCE induction in V79-E was observed only when freely dissolved BaP was added together with S9 mix. RL-19 cells were per se highly sensitive to SCE induction by BaP either freely dissolved or incorporated in liposomes. It is suggested that the incorporation of genotoxins in liposomes is a practicable method for the application, in mammalian genotoxicity assays, of agents which are barely soluble or completely insoluble in water, provided no exogenous metabolizing system is required.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000008:administration & dosage*; D002478:Cells, Cultured; D006224:Cricetinae; D003434:Crossing Over, Genetic / Q000187:drug effects*; D008081:Liposomes / Q000008:administration & dosage*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012995:Solubility
D001580:Benzopyrenes; D008081:Liposomes; D009153:Mutagens; D001564:Benzo(a)pyrene