Latency of pancreatic fluid secretory response to intestinal stimulants in the dog.
The effect of atropine and truncal vagotomy on the latency of the secretory response of the exocrine pancreas to rapid intraduodenal injection of L-tryptophan, sodium oleate and HCl or to rapid intraportal injection of secretin or CCK octapeptide has been determined in conscious dogs with pancreatic fistulae. The intraduodenal stimulants were injected either alone or in the presence of an intravenous infusion of secretin, CCK octapeptide or a combination of both hormones. The mean latency of response to tryptophan alone (62 +/- 2 s), oleate alone (64 +/- 5 s) and HCl alone (91 +/- 3 s) was significantly longer (P less than 0.05) than the latency to intraportal secretin (28 +/- 5 s) or CCK octapeptide (45 +/- 4 s). Infusion of secretin alone or with CCK octapeptide significantly shortened the latency of response to tryptophan (38 +/- 3 s) and oleate (41 +/- 5 s), but had no effect on the latency to intraduodenal HCl (96 +/- 4 s). Atropine and truncal vagotomy both increased the latency to intraduodenal tryptophan and oleate threefold but did not affect the latency to intraduodenal HCl or intraportal secretin or CCK octapeptide. These data support the hypothesis that the stimulus to pancreatic fluid secretion evoked by intraduodenal HCl is humoral rather than neural, while the responses to intraduodenal tryptophan and oleate are mediated, at least in part, via an enteropancreatic, vago-vagal, cholinergic reflex.
D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D001639:Bicarbonates / Q000378:metabolism; D002766:Cholecystokinin / Q000494:pharmacology; D004285:Dogs; D004386:Duodenum / Q000187:drug effects; D005260:Female; D006851:Hydrochloric Acid / Q000494:pharmacology; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010179:Pancreas / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D012633:Secretin / Q000494:pharmacology; D012636:Secretory Rate / Q000187:drug effects; D012844:Sincalide; D014364:Tryptophan / Q000494:pharmacology; D014628:Vagotomy
D001639:Bicarbonates; D009829:Oleic Acids; D010446:Peptide Fragments; D012633:Secretin; D019301:Oleic Acid; C013173:osteum; D001285:Atropine; D014364:Tryptophan; D002766:Cholecystokinin; D012844:Sincalide; D006851:Hydrochloric Acid