title: The araE low affinity L-arabinose transport promoter. Cloning, sequence, transcription start site and DNA binding sites of regulatory proteins.
abstract: The promoter for the gene encoding the low affinity L-arabinose uptake protein in Escherichia coli was studied. The promoter was cloned, sequenced, its transcription start site determined by S1 nuclease mapping, the proteins required for in vitro transcription were determined, and the regulatory protein binding sites located by DNase footprinting. The araE promoter shows no evidence of an operator site upstream from the CRP binding site, but otherwise it is similar to the araBAD promoter.
mesh_terms: D001089:Arabinose / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009876:Operon; D010957:Plasmids; D012333:RNA, Messenger; D012097:Repressor Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; D001089:Arabinose; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D015719:Single-Strand Specific DNA and RNA Endonucleases