Monoclonal antibodies modify acetylcholine-induced ionic channel properties in cultured chick myoballs.
Monoclonal antibodies directed against the cholinergic binding site of the acetylcholine receptor were found to alter the ion channel properties in cultured chick "myoballs." Time and dose dependent reduction in acetylcholine sensitivity was observed. Noise analysis experiments indicated a decrease in the mean single channel conductance and an increase in the mean single channel open time.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002645:Chickens; D006801:Humans; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000378:metabolism*; D009157:Myasthenia Gravis / Q000276:immunology / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007473:Ion Channels; D011950:Receptors, Cholinergic; D000109:Acetylcholine