title: The use of cluster analysis in determining possible enzyme-substrate interactions with reference to nucleoside diphosphatase activity of Golgi membranes.
abstract: The relative effectiveness of deoxyribonucleoside diphosphates, ribonucleoside diphosphates and phosphorylated B vitamins as substrates of the nucleoside diphosphatase of Golgi membranes have been determined. The substrates have been classified by cluster analysis. These findings suggest that a base ketone group and the 3'-OH of the substrates are important in promoting catalytic effectiveness, possibly by promoting hydrogen bonding during enzyme-substrate complex formation.
mesh_terms: D017766:Acid Anhydride Hydrolases; D000818:Animals; D006056:Golgi Apparatus / Q000201:enzymology*; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C023837:nucleoside-diphosphatase