Nucleotide substitutions in a yeast mitochondria cis-acting mutant located in the last intron of the apocytochrome b gene.
The region of mitochondrial DNA corresponding to the intron mutant M6-200 in Saccharomyces cerevisiae D273-10B has been isolated, and the nucleotide sequence of a 519 bp RsaI fragment has been determined. Three nucleotide substitutions were found at nucleotides +2650 (G----T), +2668 (G----A) and +2798 (A----G), all within the genetically defined location in the gene. Particular significance can be attributed to the first two changes (+2650 and +2668), that can be genetically isolated from the third substitution and, in addition, alter conserved sequence features detected in a study [(1982) Biochimie 64, 867-881] of fungal mitochondrial introns.
D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D045303:Cytochromes b; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003573:Cytochrome b Group; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D045303:Cytochromes b; D004262:DNA Restriction Enzymes