Protection of cultured mammalian cells by S-2-(3-aminopropylamino)ethylphosphorothioic acid(WR-2721).
The ability of WR-2721 to protect cultured mammalian cells against radiation-induced killing was nearly the same as that of cysteamine when WR-2721 was activated by mouse liver extract. Without the liver extract, protection by WR-2721 required long incubations with the cells prior to irradiation. The protective activity increased in proportion to the cell concentration. The dose reduction factor at a concentration of 4 mM WR-2721 was 1.11 and 1.41 for 1.5 X 10(5) cells/ml and 15 X 10(5) cells/ml of cultured cells, respectively. A non-protein bound sulfhydryl group was detected in cell suspensions after incubation with WR-2721, but it was not a dephosphorylated product of WR-2721.
D004999:Amifostine / Q000627:therapeutic use* / Q000633:toxicity; D000818:Animals; D002478:Cells, Cultured; D003037:Cobalt Radioisotopes / Q000627:therapeutic use; D003543:Cysteamine / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D007940:Leukemia L5178 / Q000532:radiotherapy*; D007942:Leukemia, Experimental / Q000532:radiotherapy*; D008099:Liver; D008624:Mercaptoethylamines / Q000627:therapeutic use; D051379:Mice; D009946:Organothiophosphorus Compounds / Q000627:therapeutic use*; D011837:Radiation-Protective Agents / Q000627:therapeutic use; D013997:Time Factors; D014020:Tissue Extracts / Q000627:therapeutic use
D003037:Cobalt Radioisotopes; D008624:Mercaptoethylamines; D009946:Organothiophosphorus Compounds; D011837:Radiation-Protective Agents; D014020:Tissue Extracts; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; D003543:Cysteamine; D004999:Amifostine