Improved method for cloning DNA complementary to minor mRNAs: preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins.
A procedure for the rapid fractionation of mRNA has been used to enrich mRNAs encoding a set of intermediate filament proteins in trophoblastoma cells. The procedure involves sucrose-gradient fractionation followed by high-resolution preparative gel electrophoresis. Part of the enriched mRNA preparation has been used to prepare a hybridization probe to screen a trophoblastoma cDNA library in Escherichia coli. A small proportion of the clones hybridized to the probe, and among these a specific clone was identified.
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D007381:Intermediate Filament Proteins / Q000235:genetics*; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D014328:Trophoblastic Neoplasms / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics
D004274:DNA, Recombinant; D007381:Intermediate Filament Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm