Repetitive DNA of Candida albicans: nuclear and mitochondrial components.
We report the isolation and analysis of the rapidly reassociating DNA of the pathogenic, dimorphic fungus Candida albicans. Minicot analysis of whole-cell repetitive DNA suggested that a significant portion of this component was mitochondrial DNA. Genomic blot hybridizations in which radioactive whole-cell repetitive DNA was used as a probe revealed eight major EcoRI bands in the molecular weight range resolved by the gel system used. Isolation and analysis of high-purity mitochondrial DNA have shown that five of these bands are of mitochondrial origin. The remaining three bands are of nuclear origin and represent repetitive sequences that are found in the nuclear genome. Attempts to isolate nuclear DNA that was completely free of mitochondrial DNA contamination were unsuccessful.
D002176:Candida albicans / Q000235:genetics* / Q000648:ultrastructure; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000032:analysis* / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012091:Repetitive Sequences, Nucleic Acid
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI