[Properties of Hodgkin cell lines. Possible significance for pathophysiology and clinical medicine].
In the last three years, five permanently in-vitro growing cell cultures with malignant properties were established from tumour material of patients with histologically confirmed Hodgkin's disease. Four cell lines have been maintained in culture. L 428 had identical characteristics in every respect with Hodgkin and Sternberg-Reed cells, tested in-vivo on biopsy tissue. The other lines--L 538, L 540 and L 591 - had certain characteristics of Hodgkin and Sternberg-Reed cells with a number of markers, but were not fully congruent. All lines reacted with a heterologous antiserum against L 428, which selectively cross-reacted with Hodgkin and Sternberg-Reed cells in fresh biopsies. Two sublines, L 428 KS and L 428 KSA, were established from L 428 by modifying the culture medium. Tests on L 428 KS cells with conventional methods and with monoclonal antibodies demonstrated that this line carried antigens of myeloid cells; however, it could not be definitely placed into any haematopoetic line. Conditioned medium of L 428 and its sublines showed CSF activity (colony-stimulating factor) and suppression of cell-mediated cytolysis.
D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001773:Blood Cells / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002869:Chromosome Aberrations; D002900:Chromosomes, Human, 1-3; D002901:Chromosomes, Human, 13-15; D002902:Chromosomes, Human, 16-18; D002904:Chromosomes, Human, 21-22 and Y; D002906:Chromosomes, Human, 6-12 and X; D005260:Female; D005312:Fetal Blood; D004854:Herpesvirus 4, Human; D006689:Hodgkin Disease / Q000503:physiopathology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009283:Naphthol AS D Esterase / Q000378:metabolism; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D011951:Receptors, Complement / Q000032:analysis; D011991:Receptors, Virus / Q000032:analysis; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000956:Antigens, Viral; D011951:Receptors, Complement; D011991:Receptors, Virus; D009283:Naphthol AS D Esterase; D000135:Acid Phosphatase
Eigenschaften von Hodgkin-Zellinien. Mögliche Bedeutung für Pathophysiologie und Klinik.