Nuclease-sensitive sites in the two major intracellular simian virus 40 nucleoproteins.
Simian virus 40 nucleoprotein isolated from the nuclei of infected cells contains a nuclease-sensitive site adjacent to the viral origin of replication (between 0.66 and 0.73 map unit). Nuclear extracts were subfractionated by sucrose gradient centrifugation to yield provirions (200S) and simian virus 40 chromatin (80S). The 80S fraction was cleaved either by DNase I or by an endonuclease endogenous to BSC-1 cells with high preference for the 0.66 to 0.73 region. The 200S fraction was treated to release core particles that were sensitive to nuclease cleavage; however, DNase I showed little or no preference for the 0.66 to 0.73 region of the provirion core nucleoprotein.
D002843:Chromatin / Q000378:metabolism; D003850:Deoxyribonuclease I; D004229:Dithiothreitol / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D008836:Micrococcal Nuclease / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D013539:Simian virus 40 / Q000032:analysis*; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
D002843:Chromatin; D009698:Nucleoproteins; D014764:Viral Proteins; D004533:Egtazic Acid; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D004229:Dithiothreitol