title: Stability of expression-linked surface antigen gene in Trypanosoma equiperdum.
abstract: African trypanosomes evade clearance in immune-competent hosts by periodically replacing their major surface glycoprotein with an antigenically different glycoprotein. Expression of many of these variant surface glycoproteins (VSGs) is associated with the duplication and transposition of silent basic copy genes (BCs) into unlinked genomic expression sites. The new expression-linked VSG gene copies (ELCs) are oriented with their 3' ends proximal to chromosome telomeres. Other VSG genes are activated without the production of an ELC. The 3' ends of these VSG genes are near chromosome telomeres both when they are active and when they are inactive. Recently, we have shown that activation of the VSG-1 gene in the BoTaR (Bordeaux trypanozoon antigen repertoire) serodeme of Trypanosoma equiperdum involves the duplication and transposition of a telomeric BC gene into one of at least three unlinked telomeric sites. Here we show that the VSG-1 ELC is inactivated but not eliminated in some antigenic variants derived from a VSG-1 expressor. In addition, a subsequent variant that again expresses VSG-1 has not reactivated the residual VSG-1 ELC (R-ELC), but instead contains a new, active VSG-1 ELC in an unlinked telomeric site. These results show that the simple presence of an ELC in a potential expression site is not sufficient for its expression.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D004251:DNA Transposable Elements; D003851:Deoxyribonucleases; D005786:Gene Expression Regulation; D005796:Genes; D008040:Genetic Linkage; D012333:RNA, Messenger / Q000235:genetics; D014345:Trypanosoma / Q000235:genetics* / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D004251:DNA Transposable Elements; D012333:RNA, Messenger; D003851:Deoxyribonucleases