Respiratory proteins from the extremely thermophilic aerobic bacterium, Thermus thermophilus. Purification procedures for cytochromes c552, c555,549, and c1aa3 and chemical evidence for a single subunit cytochrome aa3.
We have developed a chemically defined, minimal growth medium for Thermus thermophilus which is suitable for nutritional studies, isotopic enrichment, and genetic manipulation of the organism. Reliable procedures are described for the large scale purification of cytochrome c552 from the periplasm and for cytochrome c555,549 and cytochrome c1 aa3 from the plasma membrane. In contrast to a previous report (Fee, J. A., Choc, M. G., Findling, K. L., Lorence, R., and Yoshida, T. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 147-151) which suggested a molecular weight near 200,000, the cytochrome c1aa3 complex was shown by protein and amino acid analyses to have Mr approximately 93,000. Sodium dodecyl sulfate-urea-polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography, combined with amino acid analyses, revealed the presence of only two proteins in a 1:1 ratio: C-protein has Mr approximately 33,000, binds heme C, and is thought to correspond to cytochrome c1. A-protein has Mr approximately 55,000 and is thought to bind the four redox components (2 heme A and 2 Cu) of cytochrome aa3.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000302:isolation & purification*; D006736:Horses; D046911:Macromolecular Substances; D008970:Molecular Weight; D010101:Oxygen Consumption; D013824:Thermus / Q000201:enzymology* / Q000254:growth & development
D000596:Amino Acids; D003574:Cytochrome c Group; D046911:Macromolecular Substances; C024104:cytochrome c-555,549; C018887:cytochrome c-555; D003576:Electron Transport Complex IV