Complete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae.
We have sequenced the yeast HSP90 gene, which encodes the (apparent) Mr = 90,000 heat shock-inducible protein of this organism. All sequences required for the heat shock-regulated expression of this gene reside on a segment of DNA containing no more than 273 nucleotides 5' to the transcription origin. Transcript mapping reveals that the mature hsp90 mRNA contains a 59-nucleotide 5' untranslated segment, a coding segment of 2130 nucleotides (sufficient to encode a protein of Mr = 81,419), and a 3' untranslated segment of 128 nucleotides. Although the sequences surrounding the coding region of the HSP90 gene are quite similar to those surrounding other sequenced yeast genes, we find as well a limited homology between sequences located 5' to this gene and the putative heat shock-regulatory sequences located 5' to the heat shock-inducible genes of Drosophila.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes; D005796:Genes; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003062:Codon; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes