Incorporation of a voltage sensitive pore from guinea pig heart mitochondria into Black lipid membranes and characterization of electrical properties.
A pore from guinea pig heart mitochondria has been incorporated into BLM's (Black lipid membranes) in a highly oriented manner and its electrical properties studied. The pore shows multistate behaviour, the distribution of the pore between different conducting states being very sensitive to voltage. This has been proven by computation of single-pore experiments. Highest single pore conductance was 4.5 nSi in 1 M KCl, independent of voltage and with no detectable preference for cations or anions. The pore from guinea pig heart mitochondria reacts more sensitively to voltage than pores of mitochondria from other tissues so far incorporated into BLM's.
D000818:Animals; D004553:Electric Conductivity; D006168:Guinea Pigs; D007425:Intracellular Membranes / Q000502:physiology; D007473:Ion Channels / Q000502:physiology; D008081:Liposomes; D008564:Membrane Potentials; D008929:Mitochondria, Heart / Q000502:physiology*
D007473:Ion Channels; D008081:Liposomes