title: Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.
abstract: A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The specific modification of hygromycin B is a phosphorylation of the hydroxyl on the 4 position of the cyclitol ring (hyosamine). The presence of the phosphotransferase in E. coli correlates with reduced accumulation of [14C]hygromycin B.
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology*; D010770:Phosphotransferases / Q000378:metabolism; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D006921:Hygromycin B; D000123:Acetyltransferases; D010770:Phosphotransferases; D004262:DNA Restriction Enzymes