title: A recovered avian myelocytomatosis virus that induces lymphomas in chickens: pathogenic properties and their molecular basis.
abstract: The avian myelocytomatosis virus MC29 induces neoplastic diseases in chicken, including myelocytomas and tumors of kidney and liver, which are due to the action of the v-myc gene. However, MC29 has never been reported to cause lymphoid tumors, the disease associated with activation of the c-myc gene by the insertion of a lymphoid leukosis virus genome. We have analyzed a recovered MC29 virus, HBI, which has a myc gene containing c-myc sequences, acquired by recombination with the cellular gene, and some v-myc sequences. Inoculation of HBI into chickens resulted in lymphoid tumors independent of the bursa. Antigenically these tumors were made up of T and B cells. Molecular analysis showed HBI proviral DNA in 36 of 39 tumors analyzed, with no obvious alteration of c-myc, and the HBI gag-myc fusion protein, p 108, could be detected in tumor cells. These data are discussed in terms of the mechanism of target-cell specificity for transformation by the myc gene.
mesh_terms: D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics / Q000472:pathogenicity*; D001483:Base Sequence; D002645:Chickens; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D005814:Genes, Viral; D007942:Leukemia, Experimental / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D009857:Oncogenes; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D014764:Viral Proteins; D004262:DNA Restriction Enzymes