Suppression of delayed-type hypersensitivity to oxazolone in whole-body-irradiated mice and protection by WR-2721.
The effect of whole-body irradiation on cellular immunity, as measured in vivo by delayed-type hypersensitivity (DTH) to oxazolone (4- ethoxymethylene -2-phenyl- oxazol -5-one), was determined in CD2F1 mice. DTH, determined by changes in ear swelling after challenge with oxazolone, was significantly depressed in irradiated mice (500-900 rad of 60Co) in a dose-dependent fashion when animals were irradiated after sensitization and before challenge with oxazolone. Administration of WR-2721 [S-2-(3-aminopropylamino)ethylphosphorothioic acid] 30 min before irradiation (2 days after sensitization) resulted in protection against suppression of DTH, which was dependent on drug and radiation dose. An effective dose of WR-2721 (200 mg/kg body wt) provided an approximate dose-modifying factor of 1.3. The data suggest that WR-2721 interacts with cells involved in that DTH response (lymphocytes and/or macrophages) and that WR-2721 may be useful in protecting against radiation-induced decrements in cell-mediated immunity.
D004999:Amifostine / Q000008:administration & dosage*; D000818:Animals; D003037:Cobalt Radioisotopes / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004342:Drug Hypersensitivity / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000276:immunology*; D007108:Immune Tolerance / Q000187:drug effects / Q000528:radiation effects*; D007111:Immunity, Cellular / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009946:Organothiophosphorus Compounds / Q000008:administration & dosage*; D010080:Oxazoles / Q000276:immunology*; D010081:Oxazolone / Q000276:immunology*; D013997:Time Factors; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes; D009946:Organothiophosphorus Compounds; D010080:Oxazoles; D010081:Oxazolone; D004999:Amifostine