Similar rate of O6-ethylguanine elimination from DNA in normal human fibroblast and xeroderma pigmentosum cell strains not transformed by SV40.
Using a highly specific rabbit antiserum (E3) directed against O6-ethyldeoxyguanosine in a competitive radioimmunoassay, we have measured the kinetics of removal of this alkylation product from the DNA of human fibroblasts treated with the N-nitroso carcinogen N-ethyl-N-nitrosourea. A very similar rate of elimination from DNA was found in two normal human fibroblast strains (GM730 and 54BR) and in the nonvirus-transformed xeroderma pigmentosum fibroblast strains XP2BI and XP3BR. This is in contrast to the SV40-transformed xeroderma pigmentosum fibroblast strain XP12RO-SV40 which had previously been shown to be defective in its ability to remove O6-ethylguanine from DNA.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D012016:Reference Values; D013539:Simian virus 40 / Q000235:genetics*; D012867:Skin / Q000378:metabolism; D014983:Xeroderma Pigmentosum / Q000378:metabolism*
C007673:6-ethylguanine; D006147:Guanine; D004247:DNA