title: Primary structure and genetic organization of phage T4 DNA ligase.
abstract: The primary structure of phage T4 DNA ligase has been determined by DNA sequencing of a cloned restriction fragment containing its gene, and partial amino acid sequence analysis of the protein. The molecule has a Mr of 55,230, and contains 487 amino acids. The DNA sequence may also encode all of one and parts of two other, hitherto unidentified, T4 proteins. The four genes are closely packed, with overlaps between terminator and initiator codons of adjacent genes. Potential terminator and promoter sites for transcription are located within the coding sequence of one of the genes.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D011088:DNA Ligases / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008970:Molecular Weight; D011118:Polynucleotide Ligases / Q000235:genetics*; D013604:T-Phages / Q000201:enzymology* / Q000235:genetics
chemical_list: D004262:DNA Restriction Enzymes; D011088:DNA Ligases; D011118:Polynucleotide Ligases