Secretion of interferon by Bacillus subtilis.
Bacillus subtilis was transformed with a hybrid gene in which the sequence encoding the alpha-amylase signal peptide was joined by a linker to the sequence encoding mature human interferon alpha 2(IFN-alpha 2). The hybrid preprotein was cleaved precisely following the last amino acid of the alpha-amylase signal sequence and was secreted at 0.5--1 mg per liter. IFN-alpha 2, preceded by either one or six amino acids, has the same specific antiviral activity as IFN-alpha 2 itself.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics
D007370:Interferon Type I; D011498:Protein Precursors; D004262:DNA Restriction Enzymes; D000516:alpha-Amylases