title: Interactions of preimplantation mouse embryos with reovirus serotypes 1 and 3.
abstract: Mouse two-cell embryos were infected in vitro with reovirus serotypes 1/Lang and 3/Dearing, and the embryos were either implanted into pseudopregnant mice or observed in vitro for cytopathic effects. The reovirus serotypes 1/Lang and 3/Dearing differed in their capacity to kill embryos in vitro and in vivo: when embryos were infected in vitro with reovirus serotype 1/Lang and then transferred to foster mothers, pups resulted only at multiplicities of infection of a few particles per embryo. In contrast, infection of embryos with as much as 6 X 10(4) reovirus type 3 particles per embryo resulted in viable pups. In vitro, reovirus serotype 1/Lang was more virulent than serotype 3/Dearing. The infection of ovum with reovirus offers a unique model for the study of congenital infection and should yield important information concerning the molecular basis of virus virulence to maturing fetuses.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001755:Blastocyst / Q000382:microbiology* / Q000502:physiology; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000382:microbiology; D004624:Embryo Transfer; D012089:Mammalian orthoreovirus 3 / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D012087:Reoviridae / Q000276:immunology / Q000502:physiology*; D012088:Reoviridae Infections / Q000196:embryology*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral