Collateral immunoprecipitation and localization of cellular antigens using monoclonal antibody--gold conjugates.
Colloidal gold--protein complexes have been used to precipitate iodinated cell surface glycoproteins from cellular lysates. Both direct and 'sandwich' techniques with monoclonal antibodies were used. The same reagents were employed for immunolocalization of the antigenic determinants at the SEM level. The immunoprecipitation had a low background and was efficient. No washing of the precipitate was necessary. The gold-antibody precipitation (GAP) technique thus bridges the gap between immunocytochemistry and biochemistry.
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D003102:Colloids; D006046:Gold; D008855:Microscopy, Electron, Scanning; D011233:Precipitin Tests / Q000379:methods*; D011956:Receptors, Cell Surface / Q000032:analysis; D011990:Receptors, Transferrin; D014168:Transferrin
D000911:Antibodies, Monoclonal; D000941:Antigens; D003102:Colloids; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin; D006046:Gold