The role of lysine-rich proteins in the acute steroidogenic response of rat adrenal cells to ACTH.
The possibility that a lysine-rich protein was involved in the acute stimulation of steroidogenesis by ACTH was investigated using [3H] labelled lysine and isolated adrenal cells. The results demonstrated that cycloheximide inhibited steroidogenesis in a dose-dependent, rapid fashion and inhibited the incorporation of radioactive lysine into protein. However cells incubated in a lysine-free medium showed the same response to ACTH as cells incubated in a lysine-containing medium. It was also demonstrated that ACTH had no effect on the incorporation of tritiated lysine into the protein or small peptide fractions. These observations suggest that a rapidly synthesised, lysine-rich protein is not involved in the acute response to ACTH.
D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D011506:Proteins; D000324:Adrenocorticotropic Hormone; D003513:Cycloheximide; D008239:Lysine; D003345:Corticosterone