Two types of glycoprotein precursors are produced by the simian rotavirus SA11.
The rotavirus genome codes for two glycoproteins: an outer capsid structural glycoprotein (VP7, apparent molecular weight 38,000 (38K)) and a nonstructural glycoprotein (NS28K). The synthesis of these glycoproteins was analyzed in infected cells and in a cell-free system derived from rabbit reticulocyte lysates supplemented with dog pancreatic microsomes. The data showed a 37K product synthesized in the cell-free system is the precursor to the 38K glycoprotein and that the 37K polypeptide contains a cleavable signal sequence (apparent molecular weight 1.5K). The 37K polypeptide was glycosylated in vitro in the presence of microsomal membranes to a polypeptide of 38K that was immunoprecipitated by monospecific antiserum to VP7. Endo H digestion of the 38K polypeptides from either infected cells or the cell-free system produced polypeptides of identical molecular weight, 35.5K (the glycoprotein precursor lacking the signal sequence). These results were confirmed by comparative studies with a variant of SA11 that is defective in glycosylation of VP7. Similar experiments with the 20K precursor to the 29K nonstructural glycoprotein showed the 20K polypeptide contains a noncleavable signal sequence. Both glycoproteins were inserted into microsomal membranes and were processed via oligosaccharide trimming.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006023:Glycoproteins / Q000096:biosynthesis*; D000882:Haplorhini; D008861:Microsomes / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D012401:Rotavirus / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D015678:Viral Structural Proteins
D006023:Glycoproteins; D009844:Oligosaccharides; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D014764:Viral Proteins; D015678:Viral Structural Proteins