The IgG subclass responses of human lymphocytes to B-cell activators.
The induction of IgG synthesis by normal human lymphocytes in response to a range of B-cell activators has been studied by indirect cytoplasmic immunofluorescence using monoclonal antibodies to the four IgG subclasses. Considerable variation was found between cells from different donors tested with the same activator but the following trends were observed:- Epstein-Barr virus and Branhamella catarrhalis induced a predominantly IgG3 response, whereas the most prevalent cells in cultures stimulated with lipopolysaccharide and Staph. aureus were IgG2-positive; the subclass distribution obtained with pokeweed mitogen resembled that found in normal serum (IgG1 greater than 2 greater than 3 greater than 4).
D001402:B-Lymphocytes / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D009346:Neisseriaceae / Q000276:immunology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011043:Pokeweed Mitogens