title: Evolution of Ca2+- and cAMP-dependent regulatory mechanisms during ram spermatogenesis.
abstract: Calmodulin level and cAMP-dependent protein kinase activity of ram germ cells at different stages of spermatogenesis have been determined. Calmodulin levels decrease during maturation. Simultaneously, calmodulin localization changes during cell differentiation. In round, elongating, and elongated spermatids, calmodulin is closely associated with the developing acrosome; in spermatozoa, it becomes present in the postacrosome, the neck region and the tail. Protein kinase activity is relatively low in testicular cells but increases dramatically during epididymal maturation of spermatozoa. A concerted regulation by cAMP and Ca2+ of biochemical events in spermatogenic cells and spermatozoa is suggested.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000378:metabolism; D002454:Cell Differentiation; D000242:Cyclic AMP / Q000494:pharmacology*; D008297:Male; D011494:Protein Kinases / Q000378:metabolism; D012756:Sheep; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000254:growth & development / Q000378:metabolism; D013737:Testis / Q000166:cytology
chemical_list: D002147:Calmodulin; D000242:Cyclic AMP; D011494:Protein Kinases; D002118:Calcium