title: Lability of human and porcine coeruloplasmins in alkaline medium.
abstract: Denaturation of human and porcine coeruloplasmins in alkaline medium (pH 11.0) followed by neutralization and precipitation with ethanol-chloroform mixture resulted in separation of the two molecular forms: soluble and insoluble in NaCl solution. The form soluble in saline shared the properties of the native protein but remained unchanged on repeated alkaline denaturation. The occurrence of two molecular forms of plasma coeruloplasmin of different lability to alkaline medium has been suggested.
mesh_terms: D000818:Animals; D002570:Ceruloplasmin / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D004589:Electrophoresis, Disc; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation; D013552:Swine; D013997:Time Factors
chemical_list: D002570:Ceruloplasmin