Plasmid mapping computer program.
Three new computer algorithms are described which rapidly order the restriction fragments of a plasmid DNA which has been cleaved with two restriction endonucleases in single and double digestions. Two of the algorithms are contained within a single computer program (called MPCIRC). The Rule-Oriented algorithm, constructs all logical circular map solutions within sixty seconds (14 double-digestion fragments) when used in conjunction with the Permutation method. The program is written in Apple Pascal and runs on an Apple II Plus Microcomputer with 64K of memory. A third algorithm is described which rapidly maps double digests and uses the above two algorithms as adducts. Modifications of the algorithms for linear mapping are also presented.
D001483:Base Sequence; D003201:Computers; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D008722:Methods; D008838:Microcomputers; D010957:Plasmids; D012984:Software
D004247:DNA; D004262:DNA Restriction Enzymes