title: Specific binding of chartreusin, an antitumor antibiotic, to DNA.
abstract: Chartreusin, an antitumor and antibacterial antibiotic, was found to inhibit negatively superhelical DNA-relaxation catalyzed by prokaryotic topoisomerase I and conversion of the superhelical DNA into unit length linear form catalyzed by single-strand-specific S1 nuclease. The inhibitory effect of the agent was due to the binding to DNA causing the alteration of tertiary structure. To characterize the binding specificity, we investigated the protection of DNA against cleavages by various restriction endonucleases. It was evidenced that the binding of the agent is not at random and correlates to the sequence 5'CGC 3' 3'GCG 5' on DNA stretch.
mesh_terms: D000903:Antibiotics, Antineoplastic; D001412:Bacillus subtilis; D001435:Bacteriophages; D001578:Benzopyrans; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli; D006027:Glycosides
chemical_list: D000903:Antibiotics, Antineoplastic; D001578:Benzopyrans; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004279:DNA, Viral; D006027:Glycosides; D004262:DNA Restriction Enzymes; C002185:chartreusin