In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions.
The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene. A physical analysis of uvrA messenger RNA synthesis from the different deletion plasmids was performed using the S1 mapping technique. Both methods indicate that the uvrA "-10" promoter sequence is sufficient to trigger uvrA transcription. Although not essential, the "-35" region, which is overlapping with the LexA binding site, is shown to have an enhancing function, as the exposure of this region after SOS induction results in a 3- to 4-fold increase in uvrA transcription. A model is presented which accounts both for the observed basal and induced expression of the uvrA gene on a molecular level.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics*; D005686:Galactokinase / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D007700:Kinetics; D009876:Operon; D010957:Plasmids
D001426:Bacterial Proteins; D005686:Galactokinase