Localisation of 5'-nucleotidase in a rat liver cell line using a monoclonal antibody and indirect immunofluorescent labelling.
A new method of labelling anti-IgG with rhodamine while protecting the IgG binding site is described. Indirect immunofluorescence of the ectoenzyme 5'-nucleotidase using a monoclonal antibody shows that it has a punctate distribution over the whole cell surface of a cultured rat liver cell line. The density of fluorescent spots representing the surface localisation of 5'-nucleotidase and of clathrin-coated pits was analysed by a computerised counting technique. The cell surface localisation of 5'-nucleotidase differs from clathrin-coated pits and microvilli. 5'-Nucleotidase within the cell is not uniquely associated with any clearly defined organelle.
D015720:5'-Nucleotidase; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D003201:Computers; D003593:Cytoplasm / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D008099:Liver; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D009708:Nucleotidases / Q000032:analysis* / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D009708:Nucleotidases; D015720:5'-Nucleotidase