Complex forms of mitochondrial DNA in human B cells transformed by Epstein-Barr virus (EBV).
Human lymphocytes and lymphoid cell lines were analyzed for the presence of complex forms of mitochondrial DNA (mtDNA) by electron microscopy. A high frequency (9%-14.5%) of catenated dimers, circular dimers, or oligomers were found in samples from Epstein-Barr-virus-(EBV) transformed lymphoblastoid cell lines. These complex forms of mtDNA were present in much lower frequencies in lymphocytes isolated from donor blood (1.3%-4.6%). Similar low frequencies were found with primary fibroblasts (1.1%) or freshly isolated monkey liver cells (2.1%). Samples from cultures of Burkitt lymphoma (BL) cell lines of EBV-positive or -negative origin contained intermediate (5%-7%) frequencies of complex forms of mtDNA.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D002051:Burkitt Lymphoma / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004272:DNA, Mitochondrial / Q000302:isolation & purification* / Q000378:metabolism; D000882:Haplorhini; D004854:Herpesvirus 4, Human; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D008970:Molecular Weight
D004272:DNA, Mitochondrial