title: Newborn puppy cerebral acid-base regulation in experimental asphyxia and recovery.
abstract: We hypothesized that part of the newborn tolerance of asphyxia involves strong ion changes that minimize the cerebral acidosis and hasten its correction in recovery. After exposure of newborn puppies to 15 or 30 min experimental asphyxia (inhalation of gas with fractional concentration of CO2 and of O2 in inspired gas = 0.07-0.08 and 0.02-0.03, respectively), blood lactate increased to 13.2 and 23.4 mmol/l, respectively, brain tissue lactate increased to 14.4 and 19.7 mmol/kg, and cerebrospinal fluid (CSF) lactate increased to 7.6 and 14.4 mmol/l. We presume that the tissue lactate increase reflects increases in brain cell and extracellular fluid lactate concentration. The lactate increase, a change that will decrease the strong ion difference (SID), [HCO3-], and pH, was accompanied by increases in Na+ (plasma, CSF, brain), K+ (plasma, CSF), and osmolality without change in Cl-. After 60-min recovery, plasma and brain lactate decreased significantly, but CSF lactate remained unchanged. [H+] recovery was more complete than that of the strong ions due to hyperventilation-induced hypocapnia. We conclude that during asphyxia-induced lactic acidosis, changes in strong ions occur that lessen the decrease in SID and minimize the acidosis in plasma and CSF. To the extent that the increase in brain tissue sodium reflects increases in intra-and extracellular fluid sodium concentration, the decrease in SID will be less in these compartments as well. In recovery, CSF ionic values change little; plasma and brain tissue lactate decrease with a similar time course, and the [H+] is rapidly returned toward normal by hypocapnia even while the SID is below normal.
mesh_terms: D000136:Acid-Base Equilibrium; D000142:Acidosis, Respiratory / Q000503:physiopathology; D000818:Animals; D000831:Animals, Newborn; D001639:Bicarbonates / Q000097:blood; D001812:Blood-Brain Barrier; D001921:Brain / Q000503:physiopathology*; D002245:Carbon Dioxide / Q000097:blood; D004285:Dogs; D004573:Electrolytes / Q000097:blood; D005260:Female; D002534:Hypoxia, Brain / Q000503:physiopathology*; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D010100:Oxygen / Q000097:blood; D011247:Pregnancy
chemical_list: D001639:Bicarbonates; D004573:Electrolytes; D007773:Lactates; D002245:Carbon Dioxide; D019344:Lactic Acid; D010100:Oxygen