Visualization of anti-histone antibodies on SV40 minichromosomes by scanning transmission electron microscopy (STEM).
The unique capabilities of the scanning transmission electron microscope (STEM) have been used for a high resolution study of antibody binding to individual SV40 minichromosomes. A method of sample preparation has been developed which allows direct visualization of the antibody molecules in a clearly recognizable form. Using this technique, we have studied the binding of anti-H2B and anti-H3 immunoglobulins to SV40 minichromosomes. The results indicate that histones H2B and H3 are located only in the nucleosomes and are absent in the linker regions.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D006657:Histones / Q000276:immunology*; D008855:Microscopy, Electron, Scanning; D013539:Simian virus 40 / Q000235:genetics*
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D006657:Histones