A method for coupling cytomegalovirus antigens to aldehyde-fixed erythrocytes for use in passive hemagglutination.
A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable for at least 6 mth at -70 degrees C and for 5 days at 4 degrees C. This provides an inexpensive reagent having a sensitivity for anti-CMV detection which compares favorably with that of the CF test and enzyme immunoassay.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology*; D004912:Erythrocytes; D005404:Fixatives; D005976:Glutaral; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration
D000914:Antibodies, Viral; D000956:Antigens, Viral; D005404:Fixatives; D005976:Glutaral