A simple solid phase enzyme immunoassay for aldosterone in plasma and saliva.
A sensitive, solid phase enzyme immunoassay for the determination of aldosterone in plasma and saliva has been developed. For this purpose anti-aldosterone coated tubes were used. The enzyme marker was a covalently linked aldosterone/horseradish peroxidase conjugate. The assay had a limit of detection of 5 pg/assay tube. The test requires neither centrifuge nor gamma or beta counter, but only a simple photometer. The free/bound separation is based on the solid phase technique with a simple washing step. The normal values for aldosterone in plasma and saliva before and after ACTH application were determined. Saliva, being easily collected by a non-invasive, stress-free technique, is an alternative to plasma. The aldosterone levels in patients with Conn's syndrome were significantly increased in plasma and also in saliva.
D000224:Addison Disease / Q000378:metabolism; D000324:Adrenocorticotropic Hormone; D000450:Aldosterone / Q000032:analysis* / Q000097:blood; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000592:standards; D008832:Microchemistry; D011137:Polystyrenes; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis*
D011137:Polystyrenes; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone