title: The sensitivity of apical Na+ permeability in frog skin to hypertonic stress.
abstract: Na+ transport across abdominal skins of the frog species Rana esculenta and Rana pipiens was analyzed by recording short-circuit current (Isc), transepithelial conductance (Gt), and the current noise generated by the random blockage of apical Na+ channels by the diuretic, amiloride. Specific Na+ current (INa) and conductance (GNa), as reflected by the amiloride-sensitive part of Isc and Gt, respectively, were markedly depressed after addition of some osmotically active substances, like sugars or alcohols to the mucosal Na+-Ringer solution. These hypertonicity-induced reactions were fast and fully reversible, even at mucosal osmolarities of 1 Osmol. With mucosal solutions of moderate hyperosmolarity a recovery of INa and GNa was observed in presence of the osmotic gradient. This "regulatory" current showed to be carried by Na+ through the Na+-specific apical channels. Contrary to the fast current drop during the initial phase of hyperosmotic shocks, the "osmoregulation" was considerably slower. The recovery of INa was only complete at smaller osmotic gradients but became more and more suppressed at higher osmolarities. Steady-state analysis of the kinetics of the Na+-specific current revealed that the current depression by osmotic shocks obeys Michaelis-Menten kinetics. This current depression at high osmolarities, as well as during the initial phase before "osmoregulation" with small osmotic gradients, can be described in terms of a non-competitive inhibition. This was also suggested by Na+-concentration jump experiments indicating a reduction of the maximal, apical Na+ permeability as mechanism of the hypertonicity-induced drop in INa. The INa kinetics after complete "osmoregulation" were, however, indistinguishable from the isotonic control condition.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D004553:Electric Conductivity; D004560:Electricity; D006982:Hypertonic Solutions; D007473:Ion Channels / Q000187:drug effects; D010539:Permeability; D011893:Rana esculenta; D011894:Rana pipiens; D011898:Ranidae / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013312:Stress, Physiological / Q000139:chemically induced / Q000378:metabolism*; D014882:Water-Electrolyte Balance
chemical_list: D006982:Hypertonic Solutions; D007473:Ion Channels; D000584:Amiloride; D012964:Sodium