Solid-phase synthesis and some biological properties of ovine corticotropin-releasing factor.
Ovine corticotropin-releasing factor (CRF) was synthesized by solid-phase method and isolated using two purification steps: gel filtration and high performance liquid chromatography. The synthetic peptide is a potent stimulator of ACTH release, as well as cyclic AMP accumulation and release in rat adenohypophyseal cells in culture and shows highly specific binding to bovine anterior pituitary plasma membranes.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000138:chemical synthesis* / Q000378:metabolism; D005260:Female; D005563:Formic Acid Esters; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007700:Kinetics; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone; D012756:Sheep
D005563:Formic Acid Esters; D007202:Indicators and Reagents; D011956:Receptors, Cell Surface; D018019:Receptors, Corticotropin-Releasing Hormone; C000345:t-butyloxycarbonyl group; D003346:Corticotropin-Releasing Hormone