Effects of (E)-5-(2-bromovinyl)-2'-deoxyuridine on the proliferation of herpes simplex virus type 1-transformed and thymidine kinase-deficient mouse cells.
(E)-5-(2-Bromovinyl)-2'-deoxyuridine (BVDU) is a highly selective inhibitor of herpes simplex virus-1 (HSV-1). A cell line (LH-1) expressing the HSV thymidine kinase (TK) gene is extremely sensitive to BVDU, whereas the parent line that expresses the cellular TK is resistant to BVDU. A TK-deficient derivative cell line (LMTK(-] which expresses neither the cellular nor viral TK was also extremely sensitive to BVDU. This suggests that the thymidine analog, BVDU, can be phosphorylated in thymidine kinase-deficient cells by pathways other than the cellular or HSV-encoded thymidine kinase.
D000818:Animals; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004305:Dose-Response Relationship, Drug; D051379:Mice; D018139:Simplexvirus / Q000201:enzymology / Q000502:physiology*; D013937:Thymidine Kinase / Q000172:deficiency / Q000378:metabolism
C020235:brivudine; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine