Induction of an anti-viral response and 2',5'-oligo A synthetase by interferon in several thymidine kinase-deficient cell lines.
It has previously been shown that a strain of thymidine kinase (tk)-deficient mouse L-929 cells was unable to respond to murine beta-interferon by induction of an anti-viral state and synthesis of double-stranded, RNA-dependent enzymes. Sensitivity to interferon can be restored by introducing into the cells a segment of Herpes simplex virus DNA containing the viral tk gene. It is shown here that not all Ltk(-) cell strains are resistant to interferon, suggesting that expression of a tk gene is not a prerequisite for response to interferon. Introduction of various genes into the resistant Ltk(-) strain, either alone or together with DNA containing the Herpes virus tk gene, leads to restoration of interferon sensitivity only when tk-containing DNA is inserted, showing that the activation of interferon responsiveness is not an artifact of the gene transfer, selection, and cloning procedures. The results imply that a component of the Herpes virus DNA introduced into the cells is able to activate interferon sensitivity.
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004790:Enzyme Induction; D007370:Interferon Type I / Q000494:pharmacology*; D007739:L Cells / Q000201:enzymology; D051379:Mice; D018139:Simplexvirus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000172:deficiency* / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis
D007370:Interferon Type I; D014764:Viral Proteins; D013937:Thymidine Kinase; D015088:2',5'-Oligoadenylate Synthetase