Conditions for production of initiation protein for germination of Clostridium perfringens spores.
The amount of initiation protein (IP) produced by Clostridium perfringens was increased by approximately one-third by modification of Duncan-Strong sporulation medium to include 0.5% glucose, 2% proteose peptone, and 2% sodium phosphate. IP but no spores were produced by the wild type when grown at 46 degrees C and by a mutant blocked at stage 0 of sporulation. IP appears to be a vegetative cell product, perhaps an overproduced autolysin or pathogenicity factor.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002364:Caseins; D003016:Clostridium perfringens / Q000378:metabolism* / Q000502:physiology; D003470:Culture Media; D005947:Glucose; D010446:Peptide Fragments; D010710:Phosphates; D013171:Spores, Bacterial / Q000502:physiology; D013696:Temperature
D001426:Bacterial Proteins; D002364:Caseins; D003470:Culture Media; D010446:Peptide Fragments; D010710:Phosphates; C018135:proteose-peptone; D005947:Glucose; C018279:sodium phosphate