Effects of ceruloplasmin and the haptoglobin-hemoglobin complex on the oxygen-dependent reduction in growth of human diploid fibroblasts in serum-free, albumin containing medium.
We examined that growth-promoting activity of two different human albumin (HSA) preparations for human diploid fibroblasts in serum-free RITC 80-7 medium. The activity of one preparation (sample A) was affected markedly by environmental oxygen, whereas the other (sample B) was little affected. Sample B contained ceruloplasmin (Cp) and haptoglobin (Hp) as impurities. To detect the generation of superoxide anion in the media the amount of reduction of cytochrome c that is inhibited by superoxide dismutase (SOD) was determined. In an aerobic environment it was relatively large in comparison with reduction inhibited in a hypoxic environment. Reduction in the sample A with HSA-supplemented medium was relatively large in comparison with that in sample B with HSA-supplemented medium. The reduction of cytochrome c also was inhibited by Cp (25 mg/l) and catalase (4000 units/ml). Moreover, SOD, Cp, catalase and Hp.Hb (but not Hp) partially prevented oxygen-dependent reduction in growth in an aerobic environment when added to sample A HSA-supplemented medium. These results suggest that Cp and Hp.Hb act as an antioxidants in culture.
D001797:Blood Protein Electrophoresis; D002374:Catalase / Q000494:pharmacology; D002478:Cells, Cultured; D002570:Ceruloplasmin / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D003574:Cytochrome c Group / Q000037:antagonists & inhibitors; D004171:Diploidy; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006242:Haptoglobins / Q000494:pharmacology*; D006454:Hemoglobins / Q000494:pharmacology*; D006801:Humans; D007122:Immunoelectrophoresis; D010100:Oxygen / Q000378:metabolism; D012709:Serum Albumin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D003470:Culture Media; D003574:Cytochrome c Group; D006242:Haptoglobins; D006454:Hemoglobins; D012709:Serum Albumin; C035492:haptoglobin-hemoglobin complex; D002374:Catalase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D010100:Oxygen