Simultaneous differentiation of three opiate receptor subpopulations by computer modelling.
[3H]-(-)-bremazocine was displaced from guinea-pig brain membrane homogenates by three compounds having different specificity to opiate receptor subpopulations. A three site receptor model showed the best fit of the calculated to the measured value for the opiate mu (DAla2,MePhe4,Gly(ol)5-enkephalin) and the delta specific compound (DAla2,DLeu5-enkephalin). Computer modelling of data from displacement curves with the opiate kappa specific compound U-50.488H favored a two site receptor model.
D000818:Animals; D001575:Benzomorphans / Q000031:analogs & derivatives / Q000494:pharmacology; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D003201:Computers; D004745:Enkephalins / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D008954:Models, Biological; D011957:Receptors, Opioid / Q000145:classification*
D001575:Benzomorphans; D004745:Enkephalins; D011957:Receptors, Opioid; C027248:bremazocine