Effect of 6-hydroxydopamine lesions on norepinephrine-induced [3H]glycogen hydrolysis in mouse cortical slices.
The effects of norepinephrine (NE) on in vitro [3H]glycogenolysis were assessed in slices of cerebral cortex from mice whose cortical noradrenergic innervation had been severely reduced by intracisternal 6-hydroxydopamine (6-OHDA) injections. A supersensitive response to NE was observed, as demonstrated by a decrease in the EC50 of the catecholamine in the lesioned mice from 533 +/- 88 nM to 39.3 +/- 7.9 nM. This supersensitive response, observed two weeks after the lesion, was post-synaptic since isoproterenol, a beta-adrenergic agonist not accumulated by pre-synaptic uptake mechanisms, also gave an equally supersensitive response.
D000320:Adrenergic Fibers / Q000187:drug effects; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D006003:Glycogen / Q000378:metabolism*; D006868:Hydrolysis; D006892:Hydroxydopamines / Q000494:pharmacology*; D008297:Male; D009115:Muridae; D009638:Norepinephrine / Q000494:pharmacology*; D016627:Oxidopamine; D011941:Receptors, Adrenergic / Q000187:drug effects; D013569:Synapses / Q000187:drug effects
D006892:Hydroxydopamines; D011941:Receptors, Adrenergic; D016627:Oxidopamine; D006003:Glycogen; D000242:Cyclic AMP; D009638:Norepinephrine