title: HbA1C measured by a colorimetric method in normal and diabetic Nigerian subjects.
abstract: Glycosylation of haemoglobin was evaluated in 30 normal adult Nigerians and 60 diabetic adult Nigerians who were at various stages of blood glucose control, by measuring ketoamine-linked hexoses in haemoglobin. These hexoses were converted by digestion with 1 M oxalic acid for 4.5 h at 100 degrees C to 5-hydroxymethylfurfural, which was measured by reaction with 2 thiobarbituric acid. Glycosylation of haemoglobin was expressed as micromoles (mumol) of fructose per 5% haemoglobin. A mean fructose concentration of 91.0 mumol/1 (s.d. +/- 9.3) was obtained for normal subjects; that for diabetic adult Nigerians was 175.3 mumol/l (s.d. +/- 26.4). There was significant correlation between fasting blood glucose levels and glycosylated haemoglobins in both normal adults (r = 0.77, P less than 0.001) and diabetic adults (r = 0.90, P less than 0.001). The colorimetric method was found to be precise and accurate with an interassay variability of 1.44% and an intra-assay variability of 0.98%.
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D003124:Colorimetry / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006452:Hemoglobinometry / Q000379:methods; D006801:Humans; D008297:Male
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin