title: Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.
abstract: The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170. (1982] and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-like structure characteristic of viroids previously established. The central region of the native HSV has a similar structure to the conserved region found in all viroids sequenced so far except for avocado sunblotch viroid. The sequence homologous to the 5'-end of U1a RNA is also found in the sequence of HSV but not in the central conserved region.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014772:Viroids / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes