[Effect of heliomycin on the respiration and oxidative phosphorylation of the liver mitochondria of the rat].
The effect of heliomycin and known uncouplers of oxidative phosphorylation on respiration and oxidative phosphorylation was studied comparatively. Heliomycin, as well as 2,4-dinitrophenol, valinomycin and gramicidin S inhibited the mitochondrial synthesis of ATP. This process was inhibited completely by heliomycin at a concentration of 1.5 x 10(-5) M. The synthesis of inorganic pyrophosphate, the other macroergic compound, was also inhibited by heliomycin, ATPase and pyrophosphatase of uncoupled mitochondria being not inhibited by the antibiotic. Like 2,4-dinitrophenol, heliomycin stimulated the synthesis of ATPase and respiration in intact mitochondria. Probably, heliomycin inhibited the synthesis of ATP and pyrophosphate by uncoupling the processes of respiration and oxidative phosphorylation. It was shown earlier that heliomycin, a specific inhibitor of bacterial RNA synthesis, also affected energy metabolism of bacterial cells by inhibiting the synthesis of ATP and active transport.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D011756:Diphosphates / Q000378:metabolism; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D011756:Diphosphates; D011083:Polycyclic Compounds; D014475:Uncoupling Agents; C007912:heliomycin; D000255:Adenosine Triphosphate
Vliianie geliomitsina na dykhanie i okislitel'noe fosforilirovanie mitokhondriĭ pecheni krysy.