Statistical limits in Scatchard analysis.
The Scatchard graph for estimating molecular receptor capacity was recently criticized for its subjectivity, by means of several examples in which graphical methods yielded meaningless estimates of receptor capacity. We applied mathematical binding models and statistical estimation to the same examples and derived quantitative measures of the uncertainty in receptor capacity. The uncertainty was within such bounds that an approximate picture of the nature and number of sites was recoverable.
D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism*; D001569:Benzodiazepines / Q000378:metabolism*; D003975:Diazepam / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A
D008024:Ligands; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D001221:Aspartate Carbamoyltransferase; D003975:Diazepam