Desensitization and cyclic AMP turnover in S49 cells.
Measurements of cAMP accumulation and turnover have been used to quantitate desensitization of S49 wild type and variant cells to epinephrine stimulation. The extent of desensitization varied with the cell type and increased with temperature. Two traditional methods for detecting desensitization (the existence of a peak in the time course of accumulation or a diminution of the response to an agonist after prior exposure to the agent) did not always demonstrate its existence. That is, those methods always underestimated the degree of desensitization, sometimes to the extent of obscuring the phenomenon altogether.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology; D007700:Kinetics; D008223:Lymphoma / Q000378:metabolism; D051379:Mice
D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004837:Epinephrine