Positive selection vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments.
A positive selection plasmid vector ( pLA7 ), containing a unique BclI site, and its use in the facile selection of a library of overlapping DNA fragments, generated by a partial digest with Sau3A, is described. Selection depends on the 5-fluorouracil + 5' AMP resistance of upp- ush - [ pLA7ush ::cloned DNA] cells, whereas upp- ush - [ pLA7ush +] cells are sensitive under the same conditions.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D009708:Nucleotidases / Q000235:genetics; D010957:Plasmids; D012641:Selection, Genetic
D004262:DNA Restriction Enzymes; C041666:endodeoxyribonuclease BclI; D015252:Deoxyribonucleases, Type II Site-Specific; D009708:Nucleotidases