Immunocytochemical evidence for the presence of gamma 1-MSH-like immunoreactivity in pituitary corticotrophs and ACTH-producing tumours.
The presence of gamma 1-MSH has been demonstrated in bovine neuro-intermediate lobe by biochemical methods, thus suggesting that this peptide is cleaved from the cryptic region of pro-opiocortin. In this study we report the localisation of gamma 1-MSH-like immunoreactivity in the adenohypophysis of man, ox, pig, dog and guinea-pig using immunocytochemical procedures at both light and electron microscope levels. Antisera recognising the C-terminal Arg-Phe-amide and the C-terminal penta-peptide-amide of gamma 1-MSH have been used throughout this study. The immunostaining was found in all endocrine cells of the pars intermedia (where present) and in scattered cells of the pars distalis identified as corticotrophs. No gamma 1-MSH immunoreactivity was detected in rat adenohypophysis. In addition, 7 ACTH-producing tumours (1 pituitary adenoma and 6 ectopic) were investigated and shown to contain gamma 1-MSH immunoreactive cells.
D000182:ACTH Syndrome, Ectopic / Q000503:physiopathology*; D000236:Adenoma / Q000032:analysis*; D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D001984:Bronchial Neoplasms / Q000378:metabolism; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism; D002417:Cattle; D004285:Dogs; D019835:FMRFamide; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis*; D008854:Microscopy, Electron; D009842:Oligopeptides; D009384:Paraneoplastic Endocrine Syndromes / Q000503:physiopathology*; D010446:Peptide Fragments / Q000032:analysis; D010903:Pituitary Gland, Anterior / Q000032:analysis; D010911:Pituitary Neoplasms / Q000032:analysis*; D051381:Rats; D013552:Swine
D009842:Oligopeptides; D010446:Peptide Fragments; D019835:FMRFamide; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones