[Use of solid-phase radioimmunoassay (RIA) for the determination of antibodies to Aujeszky's disease virus in the blood serum of vaccinated pigs].
The specific antibodies to Aujeszky's disease virus were determined by the method of solid-phase radioimmunoassay (RIA) in the blood serums of pigs vaccinated with inactivated vaccine from three different producers. As found, in some groups of vaccinated pigs the results of RIA examination are adversely affected by the relationship of antibodies to cellular antigenic determinants. This demonstrates that, after vaccination, antibodies are produced not only against the virus antigen but also against the antigens of cells on which the vaccination virus is multiplied. These drawbacks are removed by the use of suitable cell cultures for the preparation of viral and control antigens. The antigens are applicable to RIA and ELISA examinations of blood serums in infected and vaccinated pigs. The advantages of the RIA and ELISA methods, compared with the virus-neutralization test, are described in discussion.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D011863:Radioimmunoassay; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination / Q000662:veterinary*
D000914:Antibodies, Viral
Vyuzití solid-phase radioimmunoassay (RIA) ke stanovení protilátek proti viru Aujeszkyho choroby v krevním séru vakcinovaných prasat.