[Effect of sulfhydryl reagents on mevalonate kinase partially purified from chicken liver].
Unlike other vertebrate mevalonate kinase, the enzyme partially purified from neonatal chick liver was not activated by the -SH group protectors reduced glutathione, cysteine, dithiothreitol and beta-mercaptoethanol at any concentrations assayed (0.01-10.00 mM). However, the activity was found to be sensitive to thiol group binding reagents. p-Hydroxymercuribenzoate was the most active inhibitor. At 0.1 mM concentration, p-HMB completely abolished the enzyme activity. N-ethylmaleimide (0.01-1.00 mM) was practically ineffective. Inhibition by p-HMB was temperature dependent, being more potent at 37 degrees C than at 4 degrees C.
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D017853:Phosphotransferases (Alcohol Group Acceptor); D013045:Species Specificity; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D013696:Temperature
D006902:Hydroxymercuribenzoates; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C030601:4-hydroxymercuribenzoate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase; D005033:Ethylmaleimide
Influencia de reactivos de grupos -SH sobre la mevalonato cinasa parcialmente purificada de hígado de pollo.