[Cloning of the SV40 virus genome simultaneously with yeast vector YEp 13].
Recombinant plasmides were constructed, which contained all the genome of virus SV 40 and were able to replicate in bacterial and yeast cells. The plasmides were dissimilar in the orientation of the virus SV 40 genome towards the vector YEp 13. Reversed correlation was found between molecular weight and the transformating activity of plasmides for yeast cells.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008970:Molecular Weight; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D013329:Structure-Activity Relationship
D004271:DNA, Fungal; D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Klonirovanie genoma virusa SV40 v sostave drozhzhevogo vektora YEp13.