An SV40 mammalian inductest for putative carcinogens.
This paper describes an in vitro mammalian inductest for putative carcinogens. Several chemical agents were tested using this system which relies on the induction of Simian virus 40 from SV40-transformed hamster kidney cells as an indicator of potential carcinogenic hazard. Aflatoxin B1, sterigmatocystin and aflatoxin G1 were found to be the most efficient inducers in this system followed by the polycyclic hydrocarbons 9,12-dimethylbenzanthracene and benzo[a]pyrene. In principle, this test is similar to a bacterial inductest and the results obtained in the mammalian inductest are compared to those obtained for the same compounds in the bacterial inductest. In addition, SV40 induction is known to occur in response to ultraviolet radiation and ultraviolet radiation plus the photosensitizing drug, 8-methoxypsoralen.
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D004260:DNA Repair / Q000187:drug effects; D007668:Kidney / Q000166:cytology; D008647:Mesocricetus; D009152:Mutagenicity Tests; D013539:Simian virus 40 / Q000187:drug effects* / Q000254:growth & development; D010948:Viral Plaque Assay; D014775:Virus Activation / Q000187:drug effects*
D002273:Carcinogens