Binding of transferrin-iron to the plasma membrane of a lactating rabbit mammary gland cell.
1. Cells isolated from a lactating rabbit mammary gland have been investigated for transferrin-iron receptors. The existence of these structures has been demonstrated through a specific binding with a competitor non-labelled rabbit transferrin. 2. The interaction of iron-receptor complex depends on the concentration of [59Fe]transferrin, the number of cells and the time. 3. Scatchard's plot of data indicates two classes of receptor sites: one with a binding capacity 6.48 x 10(-9) g Fe per cell and affinity constant 2.48 x 10(10) M-1 and another with 1.06 x 10(-8) g Fe per cell and 4.66 x 10(11) M-1 respectively. 4. The probable mechanism of the iron transport from blood to milk through the lactating cell was discussed.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D007501:Iron / Q000378:metabolism*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000032:analysis; D011247:Pregnancy; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D011990:Receptors, Transferrin
D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D007501:Iron