Effects of cAMP, furosemide, and ouabain on transtracheal tetracycline and chloramphenicol influx in the rat.
Using an in vitro perfusion model of the rat trachea, we studied the effects of dibutyryl cAMP (10(-3)M), terbutaline sulfate (1.7 X 10(-4)M), furosemide (10(-3)M), and ouabain (10(-3)M) on net transtracheal tetracycline and chloramphenicol influx. Submucosal cAMP and terbutaline increased transepithelial potential difference and short-circuit current and net tetracycline influx from control values of 0.496 +/- 0.066 (mean +/- SEM, n = 9) and 0.451 +/- 0.077 nM/cm2 h (n = 7) by 51 and 29%, respectively. Submucosal furosemide and ouabain decreased transepithelial potential difference, short-circuit current, and net tetracycline influx from control values by 22 and 29%, respectively. Neither cAMP, terbutaline, furosemide, nor ouabain had any effect on net chloramphenicol influx. These data suggest that drugs used in conjunction with antibiotics in patients with obstructive pulmonary disease may alter delivery of certain antibiotics into the airway lumen.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002701:Chloramphenicol / Q000378:metabolism*; D004594:Electrophysiology; D005665:Furosemide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D013726:Terbutaline / Q000494:pharmacology; D013752:Tetracycline / Q000378:metabolism*; D014132:Trachea / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
D010042:Ouabain; D003994:Bucladesine; D002701:Chloramphenicol; D005665:Furosemide; D013752:Tetracycline; D013726:Terbutaline