title: Isoelectric characteristics and the secondary structure of some nucleic acids.
abstract: The isoelectric characteristics of some nucleic acid preparations from rat liver have been examined. 10S and 4S RNA species and SV-DNA were found to have isoelectric points of 5.2, 6.0-6.7, and 4.35 respectively. The molecular charge ratios (net negative charge/nucleotide) were calculated. Using SV-DNA as a standard, these isoelectric characteristics and charge ratios have been interpreted as indicating that the 10S and 4S RNAs have 35 and 56% of the molecules involved in secondary structure.
mesh_terms: D004279:DNA, Viral; D007525:Isoelectric Focusing / Q000379:methods; D008433:Mathematics; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D013539:Simian virus 40
chemical_list: D004279:DNA, Viral; D012335:RNA, Ribosomal