Effect of vesicular stomatitis virus and Semliki Forest Virus on uptake of nutrients and intracellular cation concentration.
BHK-21 cells showed an increased ability to concentrate 2-deoxy-D-glucose (dGlc) 2 to 3 h after infection with vesicular stomatitis virus (VSV) or Semliki Forest virus (SFV), which began to be released at 2 and 3 h post-infection respectively; uptake of other nutrients was not affected in this way. Intracellular Na+ was either unchanged (VSV-infected cells) or increased (SFV-infected cells); K+ content was unchanged. These results do not support the current hypothesis that a non-specific increase in membrane permeability occurs in cells infected with rhabdoviruses or togaviruses.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D003515:Cycloleucine / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D007668:Kidney; D007700:Kinetics; D011188:Potassium / Q000378:metabolism*; D012672:Semliki forest virus / Q000235:genetics*; D012964:Sodium / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
D003515:Cycloleucine; D003513:Cycloheximide; D003847:Deoxyglucose; D012964:Sodium; D011188:Potassium