Epstein-Barr virus-specific DNase activity in nonproducer Raji cells after treatment with 12-o-tetradecanoylphorbol-13-acetate and sodium butyrate.
An Epstein-Barr virus (EBV)-specific DNase was induced in EBV nonproducer Raji cells after treatment with 12-O-tetradecanoylphorbol-13-acetate and sodium butyrate. The increase in EBV DNase activity was related to the appearance of early antigen-positive cells. The enzyme had a sedimentation coefficient of 4S and was resistant to 300 mM KCl, and its induction did not depend on viral DNA synthesis. The EBV-specific DNase activity was specifically inhibited by sera from patients who had nasopharyngeal carcinoma with high early antigen activities but not by sera from normal, healthy individuals. There was a correlation between the degree of anti-EBV DNase activity and the titers of early antigen antibody.
D000936:Antigen-Antibody Complex; D002051:Burkitt Lymphoma; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002460:Cell Line; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007106:Immune Sera; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000936:Antigen-Antibody Complex; D002087:Butyrates; D007106:Immune Sera; D010704:Phorbols; D020148:Butyric Acid; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D013755:Tetradecanoylphorbol Acetate