Effect of glucose on the induction of delta-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide.
The present work shows that allylisopropylacetamide exerts an inducing effect on delta-aminolevulinic acid synthase and ferrochelatase activities in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the inducing effect produced in both enzymes. Glucose inhibits the induction of delta-aminolevulinic acid synthase and ferrochelatase in this in vitro system. A similar effect was observed with fructose and 2-deoxyglucose. No glucose effect was observed with galactose, mannose, glycerol, pyruvate and lactate. The glucose effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. The simple in vitro method used in the present work promises to be a very useful tool for studies of regulatory mechanisms of porphyrin and heme biosynthesis in hepatocytes under normal and pathological conditions (hepatic porphyrias).
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis*; D000502:Allylisopropylacetamide / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005294:Ferrochelatase / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D006418:Heme / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology*; D008190:Lyases / Q000096:biosynthesis*; D008297:Male; D051381:Rats
D002241:Carbohydrates; D000502:Allylisopropylacetamide; D006418:Heme; D003994:Bucladesine; D003513:Cycloheximide; D000624:5-Aminolevulinate Synthetase; D008190:Lyases; D005294:Ferrochelatase; D005947:Glucose