title: Properties of soluble and particulate angiotensin-converting enzymes of rabbit lung, induced macrophage and serum.
abstract: Rabbit serum, lung and corticosteroid-induced macrophage angiotensin-converting enzymes were compared with respect to migration on polyacrylamide-gel electrophoresis, sucrose gradient centrifugation and Km. Cellular particulate enzymes solubilized by nonidet P40 had approximately half the electrophoretic mobility of soluble enzymes and a similar Km (1.2 mM). Trypsin treatment of nonidet P40 solubilized particulate enzyme converted its electrophoretic mobility to that of soluble enzyme, and rendered it non-aggregating in sucrose gradients lacking detergent, similar to soluble enzyme. Approximate molecular weights by sucrose gradient centrifugation were similar for all enzymes (135,000-158,000). The data suggest that lung and macrophage enzymes are similar and that cellular particulate enzyme may be convertible to soluble enzyme.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008168:Lung / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology*; D008970:Molecular Weight; D017830:Octoxynol; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000378:metabolism*; D011092:Polyethylene Glycols; D011817:Rabbits; D012995:Solubility; D014357:Trypsin / Q000494:pharmacology
chemical_list: D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D007703:Peptidyl-Dipeptidase A; D014357:Trypsin