Inactivation of measles and herpes simplex viruses by trypan blue.
The dye trypan blue inhibits infection of cells in tissue culture by measles and herpes simplex viruses at concentrations in excess of 0.01% (w/v) principally by direct inactivation of the virions. The inactivation is temperature-dependent (10(4) times greater at 37 degrees C than at 4 degrees C) and its mechanism appears to include inhibition of virus adsorption to cells.
D000818:Animals; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D007668:Kidney; D008459:Measles virus / Q000187:drug effects*; D018139:Simplexvirus / Q000187:drug effects*; D013696:Temperature; D014343:Trypan Blue / Q000633:toxicity*
D014343:Trypan Blue