Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB.
phoB is a positive regulatory gene for phoA, which codes for alkaline phosphatase, as well as for other genes belonging to the phosphate (pho) regulon whose expression is inducible by phosphate limitation in Escherichia coli. A hybrid plasmid that contains a phoB-lacZ fused gene was constructed in vitro. This plasmid enabled us to study phoB gene expression by measuring the beta-galactosidase level in the cells. The plasmid was introduced into various regulatory mutants related to the phosphate regulon, and phoB gene expression in these strains was studied under limited and excess phosphate conditions. It was found that the regulation of phoB expression was very similar to that of phoA expression. Expression of both genes was induced by phosphate starvation. Both genes were constitutively expressed in phoR, phoS, phoT and phoU mutants and were not expressed in a phoR-phoM double mutant. The implications of these findings for the regulatory mechanism of the pho regulon are discussed.
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator; D009154:Mutation; D010710:Phosphates / Q000494:pharmacology; D010957:Plasmids; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D010710:Phosphates; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase