Photodynamic inactivation of pseudorabies virus with methylene blue dye, light, and electricity.
Pseudorabies virus was photoinactivated with a combination of methylene blue dye, light, and electricity. Viral suspensions were mixed with variable amounts of methylene blue dye and then were placed in a current source apparatus. Total inactivation of pseudorabies virus (1.7 X 10(6) 50% tissue culture infective doses per ml) was achieved with constant mixing, a methylene blue dye concentration of 10(-4) M, and an electrical current of 12 microA for 12 min.
D004560:Electricity; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000528:radiation effects*; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology*
D008751:Methylene Blue