Use of avidin-biotin-peroxidase complex for measurement of UV lesions in human DNA by microELISA.
The avidin/biotin system was introduced into the standard enzyme-linked immunosorbent assay (ELISA) to increase its sensitivity for detecting UV lesions in human DNA. Goat anti-rabbit IgG-peroxidase used in the standard ELISA as second antibody was replaced by biotinylated goat anti-rabbit IgG plus the avidin-biotin-peroxidase complex (ABC) reagent. Sensitivity of detection of plate-fixed UV-DNA-antibody complexes was increased about 8-fold and photolesions in human DNA samples irradiated with as low a dose as 1 J/m2 UVC or a suberythemal dose of UVB light could be detected.
D000818:Animals; D001360:Avidin; D001710:Biotin; D004247:DNA / Q000032:analysis / Q000276:immunology / Q000528:radiation effects*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000378:metabolism; D011701:PUVA Therapy / Q000009:adverse effects; D009195:Peroxidase; D010544:Peroxidases / Q000378:metabolism; D011817:Rabbits; D012871:Skin Diseases / Q000188:drug therapy; D014466:Ultraviolet Rays / Q000009:adverse effects
D007106:Immune Sera; D007527:Isoenzymes; D001360:Avidin; D001710:Biotin; D004247:DNA; D010544:Peroxidases; D009195:Peroxidase