Structure and properties of a highly repetitive DNA sequence in sheep.
Cleavage of sheep DNA with the restriction endonuclease EcoR I yields three discrete size classes (370, 435 and 800bp) of highly repetitive DNA. The 435bp long fragment was cloned and its nucleotide sequence determined. All three classes of repetitive DNA are related to each other as seen by cross-hybridisation. They are tandemly arranged in the genome and in situ hybridisation to sheep lymphocyte chromosomes show their location mainly in the centromere region of all chromosomes. The primary sequence of the repetitive DNA shows a close structural similarity to the bovine 1.715 satellite DNA, however only poor cross-hybridisation between the sheep and cattle repetitive DNA could be shown.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics; D008214:Lymphocytes / Q000502:physiology; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity
D004276:DNA, Satellite; D004247:DNA; D004262:DNA Restriction Enzymes