title: Construction and characterization of plasmid vectors for cloning in Staphylococcus aureus and Staphylococcus carnosus.
abstract: Several plasmid vectors for cloning in Staphylococcus aureus and S. carnosus have been constructed and characterized. The chimeric plasmids are composed of parts of the following parental plasmids: The chloramphenicol-resistance plasmid, pC194, the tetracycline-resistance plasmid, pMK148, and the erythromycin-resistance plasmid, pE12. All the chimeric plasmids confer two selectable antibiotic-resistance markers on host cells. Insertional inactivation of the various antibiotic-resistance markers occurred at the BclI site of pE12, and the Sau96- or AvaII-site of pMK148; only a slight inactivation of the chloramphenicol-resistance marker occurred at the HaeIII-site of pC194. The chimeric plasmids pCT20 and pCE10 are both stable in S. aureus and S. carnosus. In addition, the hybrid plasmids of pCT20 and pCE10, containing lambda-DNA fragments in various restriction sites between 0.4 and 1.2 kb, are stably maintained. The inserted lambda-DNA fragments appear unchanged.
mesh_terms: D002701:Chloramphenicol / Q000494:pharmacology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology; D005822:Genetic Vectors; D010957:Plasmids; D013210:Staphylococcus / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D004274:DNA, Recombinant; D004917:Erythromycin; D002701:Chloramphenicol; D013752:Tetracycline