title: Serum factors from patients with systemic lupus erythematosus enhancing superoxide generation by normal neutrophils.
abstract: It has been suggested that human neutrophils exposed to performed immune complexes or activated complement fragments generate O2- anions in extracellular medium. In vivo studies have revealed that oxygen intermediates produced by immune complex-activated neutrophils play an important role in subsequent tissue damage. Since it is difficult to obtain direct evidence that O2- is released into plasma in patients with systemic lupus erythematosus (SLE), we studied the capacities of their sera to stimulate O2- release by human neutrophils in vitro. Sera from patients with SLE significantly enhanced O2- generation by neutrophils compared to normal sera. The enhancing activity of serum in the induction of increased O2- generation correlated positively with the presence of serum immune complexes and negatively with serum complement levels. The enhancing factors were analyzed by serum fractionation on Sephadex G-200 gel filtration, and were concluded to be immune complexes of intermediate size containing an activated complement fragment.
mesh_terms: D003574:Cytochrome c Group / Q000097:blood; D005615:Freezing; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000097:blood*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000097:blood*
chemical_list: D003574:Cytochrome c Group; D013481:Superoxides; D013482:Superoxide Dismutase; D010100:Oxygen