title: Unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis.
abstract: Viral deoxyribonucleic acid extracted from a limited number of cells infected with canine adenovirus type 1 or type 2 was cleaved with several restriction endonucleases. Agarose gel electrophoresis of the limit digests showed stable differences between the canine adenovirus type 1 and type 2 cleavage patterns. Rapid and accurate typing of large numbers of clinical isolates may thus be done by deoxyribonucleic acid restriction-endonuclease analysis.
mesh_terms: D000256:Adenoviridae / Q000145:classification* / Q000235:genetics; D000257:Adenoviridae Infections / Q000382:microbiology / Q000662:veterinary; D000818:Animals; D004262:DNA Restriction Enzymes / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs / Q000382:microbiology*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005814:Genes, Viral; D007668:Kidney; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes