Changes in benzodiazepine receptors by acute stress: different effect of chronic diazepam or RO 15-1788 treatment.
In rats submitted to acute stress by forced swimming there was an immediate sharp decline in the binding of [3H]flunitrazepam and of [3H]ethyl-beta-carboline-3-carboxylate in synaptosomal membranes of the cerebral cortex and hippocampus. This was followed by a rebound increase in benzodiazepine receptors after 60 min, specially in the hippocampal formation. These two changes were blocked by chronic treatment with diazepam for 14 days (4 mg/kg/day). On the other hand, long-term treatment with the antagonist RO 15-1788 did not modify the changes caused by the acute stress. These findings are discussed in relation to the possible regulation of benzodiazepine receptors by agonists and antagonists.
D000818:Animals; D001569:Benzodiazepines / Q000037:antagonists & inhibitors / Q000378:metabolism; D001570:Benzodiazepinones / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects; D003975:Diazepam / Q000494:pharmacology*; D005260:Female; D005442:Flumazenil; D005445:Flunitrazepam / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011963:Receptors, GABA-A; D017981:Receptors, Neurotransmitter / Q000187:drug effects; D013312:Stress, Physiological / Q000378:metabolism*
D001570:Benzodiazepinones; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D017981:Receptors, Neurotransmitter; D001569:Benzodiazepines; D005442:Flumazenil; D005445:Flunitrazepam; D003975:Diazepam