Formation of phosphodiesters in thymidine monophosphate by styrene oxide.
Three products were isolated by high-performance liquid chromatography (HPLC) from the reaction between thymidine-5'-monophosphate (TMP) and styrene oxide in aqueous solution. None of the products was digested by alkaline phosphatase, but each was digested by vernom phosphodiesterase. The digestion products were identified by their mobility in HPLC and UV-spectra as phenylethylene glycol, thymidine, TMP, and hydroxyethylphenyl phosphoric acid, of which phenylethylene glycol and TMP were most abundant.
D000469:Alkaline Phosphatase; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds; D004951:Esterification; D004988:Ethers, Cyclic; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D013056:Spectrophotometry, Ultraviolet; D013938:Thymidine Monophosphate; D013942:Thymine Nucleotides
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; C013690:styrene oxide; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I