title: Direct hybridization of labeled DNA to DNA in agarose gels.
abstract: A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive probe, and the hybridization pattern visualized by autoradiography. This method is sensitive enough to detect single or low copy number DNA sequences in the genome of higher eucaryotes.
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes