Effects of lysosomotropic amines on human polymorphonuclear leucocyte function.
Lysosomotropic agents interfere with lysosome function. We studied the effects of the lysosomotropic amines: lidocaine, diphenylamine and dansylcadaverine on several functions of human polymorphonuclear leucocytes (PMN): enzyme release, phagosome-lysosome fusion, superoxide anion generation upon stimulation with opsonized bacteria, and phagocytosis and killing of opsonized Staphylococcus aureus. Lidocaine depressed all cellular functions tested. Diphenylamine reduced enzyme release and phagosome-lysosome fusion in phagocytosing PMN. This was accompanied by an increase in superoxide anion generation. Dansylcadaverine enhanced enzyme release and phagosome-lysosome fusion, and reduced superoxide anion generation. Neither of these two agents influenced bacterial uptake; bacterial killing was impaired only in dansylcadaverine treated cells. Cadaverine, an analogue that does not penetrate cells, had no effect on any of the functions tested.
D000588:Amines / Q000494:pharmacology*; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D004159:Diphenylamine / Q000494:pharmacology; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D013211:Staphylococcus aureus; D013481:Superoxides / Q000378:metabolism
D000588:Amines; D013481:Superoxides; D008012:Lidocaine; D004159:Diphenylamine; C008542:monodansylcadaverine; D002103:Cadaverine