Changes concerning the external structure of peritoneal macrophages, due to the DQ-12 standard dust.
The cytotoxic activity of the DQ-12 standard dust on the external structure of peritoneal macrophages, after intraperitoneal introduction, was investigated, vs nontreated macrophages, and kept in in vitro cultures for 3, 6, and 24 hr. The alterations related to the external structure were investigated by scanning electron microscopy (SEM), clearly showing that cell damage can take place when the dust contacts the external surface of the cell, as already shown by the work of Johnson and Davies (paper presented at the International Workshop on the in Vitro Effects of Mineral Dusts, South Wales, U.K., 1979) and Miller (Environ. Res. 20, 162-182, 1979).
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D004391:Dust / Q000009:adverse effects*; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains; D012822:Silicon Dioxide / Q000633:toxicity*
D004391:Dust; D012822:Silicon Dioxide