Blockage of ovulation in the explanted hamster ovary by a collagenase inhibitor.
Hamster ovaries explanted at 21:00 h on the day of pro-oestrus were incubated with talopeptin, a potent microbial inhibitor of collagenases, until 10:30 h on the next morning. A talopeptin concentration of 0.16 mM completely blocked ovulation in vitro. The blocking effect decreased markedly in ovaries excised at 23:00 h or later. The results suggest that proteolysis by collagenases is indispensable for the ovulatory process in the hamster.
D000818:Animals; D006224:Cricetinae; D046508:Culture Techniques; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology*; D008647:Mesocricetus; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors*; D010053:Ovary / Q000502:physiology; D010060:Ovulation / Q000187:drug effects*; D011247:Pregnancy; D011359:Proestrus
D006020:Glycopeptides; C037819:talopeptin; D003012:Microbial Collagenase