beta-RTX. A receptor-active protein from Russell's viper (Vipera russelli russelli) venom.
A single chain polypeptide, termed beta-RTX, with an apparent Mr = 9600 has been isolated from the venom of Vipera russelli russelli. It was purified by cation exchange chromatography, followed by preparative isoelectric focusing and chromatofocusing. Purity was confirmed by gel filtration, high performance liquid chromatography, gel electrophoresis, and analytical ultracentrifugation. Amino acid analysis revealed the presence of eight half-cystines, one being located at the NH2 terminus, which are linked to form four intramolecular disulfide bridges. Chromatofocusing revealed some microheterogeneity yielding three isoforms with pI values of 9.3, 9.37, and 9.48, respectively. In its native configuration, beta-RTX was not susceptible to tryptic degradation but was readily digested after reduction and alkylation. beta-RTX possesses weak phospholipase A2 activity and competes with the binding of monoamines and opiate ligands to their respective receptors. No binding to histamine, gamma-aminobutyric acid, benzodiazepine, or muscarinic receptors was observed. In vivo, whereas 100 micrograms/kg intravenous beta-RTX seemed to be without apparent effects in the rat, 10 ng/kg beta-RTX injected intracerebroventricularly caused marked sedation, with full recovery within 3 h.
D000818:Animals; D001667:Binding, Competitive; D005260:Female; D006993:Hypnotics and Sedatives; D007276:Injections, Intraventricular; D036341:Intercellular Signaling Peptides and Proteins; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism; D011957:Receptors, Opioid / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D014757:Viper Venoms / Q000032:analysis*
D006993:Hypnotics and Sedatives; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011954:Receptors, Dopamine; D011957:Receptors, Opioid; D011985:Receptors, Serotonin; D014757:Viper Venoms; C037736:beta-RTX