Determination of the electric potential at the external and internal bilayer-aqueous interfaces of the human erythrocyte membrane using spin probes.
Partitioning of oppositely charged amphipathic spin probes indicates that the electric potential at the external bilayer-aqueous interface of the human erythrocyte is insignificant, and that protruding sialic acids do not contribute to this potential. This potential at the surface is distinguished from the electrokinetic potential due to all charged groups within the hydrodynamic surface of shear. By contrast, using inside-out erythrocyte membrane vesicles, a substantial potential is observed at the cytoplasmic membrane surface. This can be attributed to the asymmetric distribution of acidic phospholipids on the two sides of the erythrocyte membrane bilayer.
D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000502:physiology* / Q000648:ultrastructure; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D008051:Lipid Bilayers; D008564:Membrane Potentials; D009439:Neuraminidase; D012794:Sialic Acids / Q000097:blood; D013113:Spin Labels
D008051:Lipid Bilayers; D012794:Sialic Acids; D013113:Spin Labels; D009439:Neuraminidase