Solubilization and partial characterization of adenosine binding sites from rat brainstem.
Binding sites for adenosine were solubilized from rat brainstem membranes with either sodium cholate, sodium deoxycholate or 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate. About 30% of the binding activity were released by [3H]phenylisopropyladenosine (PIA) binding. Specific [3H]PIA binding to the solubilized fraction was saturable and was found to be a monophasic saturation profile. In contrast, [3H]PIA binding to the brainstem membranes exhibited a biphasic profile suggesting the presence of two binding sites. By gel filtration on a Sepharose CL-6B column, the adenosine binding site--detergent complex was estimated to have app. Mr 280000 and rs = 5.4 nm.
D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D003902:Detergents; D066298:In Vitro Techniques; D010660:Phenylisopropyladenosine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D011983:Receptors, Purinergic; D012995:Solubility
D003902:Detergents; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D010660:Phenylisopropyladenosine