Phosphorylation of four acyclic guanosine analogs by herpes simplex virus type 2 thymidine kinase.
Four acyclic guanosine analogs, the (R) and (S) enantiomers of 9-(3,4-dihydroxybutyl)guanine, 9-(4-hydroxybutyl)guanine, and acyclovir were compared in enzyme kinetic experiments, using purified herpes simplex virus type 2 thymidine kinase. All four analogs showed competitive patterns of inhibition in the phosphorylation of thymidine by the viral thymidine kinase, but different affinities and relative rates of phosphorylation were observed.
D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D010766:Phosphorylation; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000378:metabolism*; D010948:Viral Plaque Assay
D006151:Guanosine; D013937:Thymidine Kinase