Saturated and unsaturated 1-0-alkyl-2-0-acetoyl-sn-glycero-3-phosphocholines and 2-lyso derivatives from ratfish liver oil. Effect on adenosine 3':5'-cyclic monophosphate concentration in hepatocyte suspensions.
1-0-Alkyl-2-0-acetoyl-sn-glycero-3-phosphocholines, containing saturated or unsaturated alkyl moieties and their 2-lyso derivatives, prepared from ratfish liver oil (Hydrolagus colliei), were studied for their in vitro effect on cellular adenosine 3':5'-cyclic monophosphate (cAMP) concentration in freshly isolated hepatocytes from adult rats. We report here for the first time that semisynthetic platelet-activating factor (PAF), the saturated and unsaturated compound, from 10(-6) M to 10(-12) M, is effective in lowering cAMP concentrations by 50% after 4 h of incubation. This effect becomes apparent after 30 min. Concentrations from 10(-12) M up to 10(-20) M did not influence cAMP levels. The 2-lyso derivatives (10(-8) M) of both compounds are equally active as compared to their 2-0-acetoyl derivatives, in lowering cAMP levels after 4 h of incubation. We discuss the existence of a transacetylating enzyme, generating the active form in rat liver cells.
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D005395:Fish Oils; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D005395:Fish Oils; D010972:Platelet Activating Factor; C026613:1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine; D000242:Cyclic AMP