title: [Isolation and characteristics of a mutant herpes simplex virus type 1 resistant to phosphonoacetic acid].
abstract: By successive passages and triple cloning of herpes simplex virus type 1 (HSV-1) in Vero cell culture in the presence of increasing concentrations of phosphonoacetic acid (PAA) a mutant of HSV-1 resistant to PAA (PAAr) was derived and characterized. The resistance to the inhibitor was transmitted from PAAr-mutant to a sensitive strain (L2) by recombination performed by the marker rescue method using DNA fragmented by Hpa-1 restrictase. The resulting recombinant (R-551) was resistant to the inhibitor and had an altered primary structure of DNA.
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial; D005819:Genetic Markers; D009154:Mutation; D009943:Organophosphorus Compounds / Q000037:antagonists & inhibitors*; D010746:Phosphonoacetic Acid / Q000037:antagonists & inhibitors*; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D005819:Genetic Markers; D009943:Organophosphorus Compounds; D010746:Phosphonoacetic Acid
vernacular_title: Vydelenie i kharakteristika mutanta virusa gerpesa prostogo tipa 1, rezistentnogo k fosfonouksusnoĭ kislote.