Construction of a gene library from the nitrogen-fixing aerobe Azotobacter vinelandii.
We have cloned the DNA of Azotobacter vinelandii in the cosmid pHC79. Recombinant cosmids that can transform Escherichia coli leuB- to a Leu+ phenotype, as well as those having sequence homology to the nitrogenase structural genes of Klebsiella pneumoniae have been selected from this library.
D001395:Azotobacter / Q000235:genetics*; D003470:Culture Media; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000096:biosynthesis; D005798:Genes, Bacterial; D007930:Leucine / Q000378:metabolism; D009591:Nitrogenase / Q000378:metabolism; D010957:Plasmids; D014161:Transduction, Genetic; D014170:Transformation, Genetic
D003470:Culture Media; D004269:DNA, Bacterial; D009591:Nitrogenase; D004262:DNA Restriction Enzymes; D007930:Leucine