title: Caffeine inhibition of aflatoxin production: mode of action.
abstract: Evaluation of caffeine and a number of related methylxanthines indicated that the ability of the compound to inhibit growth and aflatoxin production by Aspergillus parasiticus is highly specific and does not involve an inhibition of cyclic AMP phosphodiesterase. Supplementation of the culture medium with purine bases, nucleosides, and nucleotides suggested that the inhibition of fungal growth could be partially overcome by adenine or guanine but that the purines had little effect on the inhibition of aflatoxin production. Likewise, increasing the levels of trace minerals did not overcome the inhibition of toxin production. Electron microscopic evaluation of caffeine-treated and -untreated cultures indicated that the compound produced observable changes in the ultrastructure of the fungus.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors; D000225:Adenine / Q000494:pharmacology; D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002110:Caffeine / Q000494:pharmacology*; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D006147:Guanine / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013806:Theophylline / Q000494:pharmacology; D014131:Trace Elements / Q000494:pharmacology
chemical_list: D000348:Aflatoxins; D014131:Trace Elements; D002110:Caffeine; D006147:Guanine; D013806:Theophylline; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000225:Adenine