The increase in vivo by electric currents of the 99mTc-methylene diphosphonate uptake and cyclic AMP content of the bone of the cat mandible.
Technetium-99-methylene diphosphonate (99mTc-MDP) bone images were obtained from live cats whose mandibles had been stimulated non-invasively and unilaterally with electric current (12 +/- 2 microA) for 1 week. The images were obtained using a gamma camera interfaced to a digital computer for quantitative comparison of the mandible. Radionuclide uptake by the stimulated sides increased by 59 +/- 26 per cent when compared with the contralateral control sites. Immunohistochemical staining of mandibular sections for adenosine 3',5'-monophosphate (cyclic AMP) showed that periosteal osteoblasts opposite the electrodes were intensely stained. Thus 99mTc-MDP scintigraphy is a sensitive non-invasive technique for determining in vivo enhanced bone remodelling activity. The immunohistochemistry indicates that the stimulation of bone cells was limited to surfaces adjacent to both cathode and anode.
D000818:Animals; D001846:Bone Development; D002415:Cats; D000242:Cyclic AMP / Q000378:metabolism*; D004164:Diphosphonates / Q000378:metabolism*; D004558:Electric Stimulation; D005260:Female; D008334:Mandible / Q000000981:diagnostic imaging / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism; D010521:Periosteum / Q000378:metabolism; D011877:Radionuclide Imaging; D013667:Technetium / Q000378:metabolism*; D017556:Technetium Compounds
D004164:Diphosphonates; D017556:Technetium Compounds; D013667:Technetium; C029045:technetium Tc 99m diphosphonate; D000242:Cyclic AMP