Characterization of the FBR-murine osteosarcoma virus complex: FBR-MuSV encodes a FOS-derived oncogene.
The FBR murine osteosarcoma virus complex, isolated from a radiation-induced osteosarcoma of an X/Gf mouse causes the rapid appearance of osteosarcomas in newborn mice and transforms fibroblasts in vitro. The two components of the FBR-viral complex have been isolated separately in tissue culture: FBR-MuLV by end-point dilution and FBR-MuSV by the establishment of mouse [FBR-NP 117 (NIH 3T3)] and rat non-producer cell lines [FBR-NP415 (REF)]. The host range and RNase Tl fingerprint analysis of FBR-MuLV demonstrated a pattern closely related to, but distinguishable from, Akv-MuLV. Transformed cells from both mice and rats contain a rescuable FBR-MuSV genome. These pseudotypes produce foci in tissue culture and induce osteosarcomas in susceptible mouse strains. An FBR-MuSV (FBR-MuLV) cDNA probe detects a 5.2 kb HindIII and a 9.5 kb EcoRI FBR-MuSV-specific fragment in FBR-MuSV-transformed non-producer rat cells. The same fragments hybridized with a fos specific probe, demonstrating that FBR-provirus contains a c-fos-derived onc-gene.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics* / Q000382:microbiology; D051381:Rats; D011919:Rats, Inbred Strains; D009053:Sarcoma Viruses, Murine / Q000032:analysis / Q000235:genetics*; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000382:microbiology
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII