title: Purification and characterization of two kinds of porins from the Enterobacter cloacae outer membrane.
abstract: Two major outer membrane proteins of Enterobacter cloacae 206 were purified and identified as porins by using reconstituted vesicles. The 37-kilodalton porin forms a channel with a radius of 0.6 nm, which prefers positively charged substances to negatively charged ones, whereas the 39- to 40-kilodalton porin forms a larger channel with a radius of 0.8 nm, which has weaker selectivity for electric charges.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D002463:Cell Membrane Permeability; D002511:Cephalosporins / Q000378:metabolism; D004754:Enterobacter / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D018272:Porins; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D008565:Membrane Proteins; D018272:Porins