Fluorescent localization of calcium at sites of cell attachment and spreading.
Chlorotetracycline (CTC), a calcium-chelating fluorescent probe identifies and localizes calcium when applied to cultured neural crest cells. The fluorescence occurs at specific membrane regions involved in cell attachment and spreading as well as with the formation of cytoskeletal stress fibers (actin microfilament bundles). The observed CTC reaction indicates that calcium sequestration and the development of these membrane-cytoskeletal features share a temporal and spatial pattern. Thus, the selective availability of calcium may be an important determinant of cell morphogenesis.
D000557:Ambystoma; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D002751:Chlortetracycline / Q000494:pharmacology; D046508:Culture Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D009432:Neural Crest
D002614:Chelating Agents; D007473:Ion Channels; D002118:Calcium; D002751:Chlortetracycline