Inhibition study of ADP,ATP transport in mitochondria with trinitrophenyl-modified substrates.
The ADP,ATP carrier of rat liver mitochondria is specifically inhibited by Meisenheimer-type trinitrophenyl (TNP) derivatives of ADP and ATP. Due to a systematic inhibition study we could show that the TNP-moiety itself, even in the 2', 3'-O-cyclic Meisenheimer complex, revealed no inhibition of mitochondrial ADP,ATP transport. Nucleosidic TNP-compounds are weak inhibitors. Introduction of a phosphate group at the 5'-position, however, enhances the inhibitory power markedly. Our findings point to a synergistic effect of the 5'-phosphate chain and the TNP-moiety. Irreversible inhibition by TNP-nucleotides can be ruled out to the fact that the inhibited ADP,ATP-transport system is fully reactivated by addition of albumin.
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors*; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains
C009587:2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate; D000244:Adenosine Diphosphate; C035319:2',3' O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases