Isolation and analysis of aroFo mutants by using an aroF-lac operon fusion.
The lac structural genes were fused to the regulatory region of the aroF-tyrA operon so that the expression of beta-galactosidase was regulated by the tyrR+ gene product. Transducing phage carrying the aroF-lac fusion were isolated, and a lambda aroF-lac lysogen was used to select for aroFo mutants. A plasmid vector was constructed onto which the aroFo mutations were transferred by recombination in vivo.
D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D009154:Mutation; D009876:Operon; D010957:Plasmids; D011995:Recombination, Genetic; D014161:Transduction, Genetic