title: Plasmid visualization and nif gene location in nitrogen-fixing Azospirillum strains.
abstract: A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all eight strains investigated and not on endogenous plasmids.
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology*; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D005798:Genes, Bacterial; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics
chemical_list: D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI