title: Avidin is induced in chicken embryo fibroblasts by viral transformation and cell damage.
abstract: Synthesis and secretion of avidin was studied in cultured chicken embryo fibroblasts infected with transforming retroviruses (Rous sarcoma virus, its mutants temperature-sensitive for transformation, OK-10 virus) or a nontransforming retrovirus (RAV-1). Avidin was detectable in both transformed and untransformed cultures, and was identical to chicken egg white avidin by several criteria: biotin-binding, heat-induced biotin exchange, subunit size (mol. wt. 15 600), immunoprecipitation of metabolically labeled proteins and immunoblotting. Transformation increased the production of avidin up to 50-fold, but several experiments suggested that the induction was not a direct consequence of virus-induced cell transformation. The production of avidin seemed to relate to cellular damage both in cultures of virus-transformed and of normal fibroblasts. It may represent a response to cellular damage and viral transformation may activate the process.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001360:Avidin / Q000235:genetics* / Q000302:isolation & purification; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D005347:Fibroblasts / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D008970:Molecular Weight; D009154:Mutation; D010047:Ovalbumin / Q000031:analogs & derivatives*
chemical_list: D001360:Avidin; D010047:Ovalbumin