Human papovavirus infection of an SV40-transformed human embryonic kidney cell line.
Human embryonic kidney cells were transformed at their third passage with an origin-defective mutant of SV40, 1-11. One clone of these cells, SV1, demonstrated several phenotypes of transformation including high-density growth and anchorage independence. The SV1 cells also were 100% T antigen-positive when tested by immunofluorescence and immunoprecipitation assays. These cells with the SV40 T protein were able to support the persistent replication of BK viral DNA but did not support virus multiplication. The SV1 cells would also support the replication of the Sau 3A A fragment of the BK genome cloned into pBR 322. However, SV1 cells would not support multiplication of JC virus or replication of JC DNA.
D002460:Cell Line; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004587:Electrophoresis, Agar Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D007577:JC Virus / Q000502:physiology; D007668:Kidney; D013539:Simian virus 40 / Q000502:physiology*; D014776:Virus Cultivation; D014779:Virus Replication