Non-invasive near infrared spectroscopy of brain in fluorocarbon exchange-transfused rats.
Near infrared spectra (700-930 nm) were recorded from the head of anesthetized rats before and after blood substitution with a fluorocarbon emulsion. A large band, centered at 850 nm and characteristic of oxidized cytochrome a, a3, was evidenced in hemoglobin-free living animals. Variations in the amplitude of this absorption band were observed under different respiratory conditions.
D000818:Animals; D001794:Blood Pressure; D001803:Blood Transfusion; D001923:Brain Chemistry; D002560:Cerebrovascular Circulation; D003576:Electron Transport Complex IV / Q000032:analysis*; D005465:Fluorocarbon Polymers; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D051381:Rats; D013055:Spectrophotometry, Infrared / Q000379:methods*
D005465:Fluorocarbon Polymers; D006454:Hemoglobins; D003576:Electron Transport Complex IV