The TGGCA-binding protein: a eukaryotic nuclear protein recognizing a symmetrical sequence on double-stranded linear DNA.
Low salt extracts of chicken oviduct nuclei contain a DNA binding protein with high affinity for specific DNA sequences in the flanking regions of the chicken lysozyme gene. Two of the three binding sites found within a total of 11 kb upstream from the promoter are located only 92 bp apart from each other. Upon comparison of the DNA binding sites, the symmetrical consensus sequence 5'- TGGCANNNTGCCA -3' can be deduced as the protein recognition site. This sequence is the central part of 23 to 25 base pairs protected by the DNA binding protein from DNAase I digestion. A homologous binding activity can be detected in nuclei from several chicken tissues and from mouse liver.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D005796:Genes; D008099:Liver / Q000378:metabolism; D009113:Muramidase / Q000235:genetics; D009698:Nucleoproteins / Q000378:metabolism*; D009928:Organ Specificity; D010057:Oviducts / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity
D004268:DNA-Binding Proteins; D009698:Nucleoproteins; D004247:DNA; D004262:DNA Restriction Enzymes; D009113:Muramidase