The beta-adrenoceptor of pig coronary arteries: determination of beta 1 and beta 2 subtypes by radioligand binding.
beta-Adrenoceptors of pig coronary arteries were investigated by the use of a new ligand, 125iodocyanopindolol (ICYP) in binding studies. Inhibition of ICYP binding by betaxolol (a selective beta 1-antagonist), zinterol (a selective beta 2-agonist) and ICI 118551 (a selective beta 2-blocking drug) resulted in non-linear Scatchard plots, suggesting that both beta-adrenoceptor subtypes are present in pig coronary arteries. Computer analysis of the data gave a beta 1:beta 2-adrenoceptor ratio of approximately 65:35.
D000818:Animals; D003331:Coronary Vessels / Q000032:analysis*; D066298:In Vitro Techniques; D020120:Iodocyanopindolol; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D013552:Swine
D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol