[3H]-dynorphin A (1-9): binding characteristics and degradation profile in brain homogenates.
[3H]-Dynorphin A (1-9) is rapidly degraded in brain homogenates, even at 0 degree C. Although protection from degradation at O degree C, and to a lesser extent at 25 degrees C, was obtained with a combination of bestatin, L-leucyl-L-leucine, L-arginyl-L-arginine and captopril, these peptidase inhibitors had little effect on binding at 0 degree C. At the kappa-site, the maximum binding capacity of [3H]-dynorphin A (1-9) at 0 degree C was similar to that at 25 degrees C but it is suggested that, even after suppression of mu- and delta-binding, [3H]-dynorphin A (1-9) may bind to more than one site for which it has differing affinities at 0 degree C.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004399:Dynorphins; D004723:Endorphins / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D010446:Peptide Fragments / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D011957:Receptors, Opioid / Q000378:metabolism*; D017473:Receptors, Opioid, kappa
D004723:Endorphins; D010446:Peptide Fragments; D011480:Protease Inhibitors; D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa; C030738:dynorphin receptor; D004399:Dynorphins; C040238:dynorphin A (1-9)