Selective defect in human neutrophil superoxide anion generation elicited by the chemoattractant N-formylmethionylleucylphenylalanine in pregnancy.
Pregnancy has been associated with alterations of polymorphonuclear neutrophil (PMN) function. Superoxide anion production was studied in pregnant women paired with nonpregnant women of childbearing age. There was a significant decrease in the amount of cytochrome c reduced in response to 1 microM N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) but not to phorbol myristate acetate, 4 or 20 ng/ml. Chemotaxis was also depressed. Binding of tritiated fMet-Leu-Phe to PMNs from pregnant women was not defective. Incubation of normal cells in up to 10(-6) M estradiol or progesterone did not mimic the defect, but 10(-7) M progesterone caused a decrease in chemotaxis. Serum pooled from women with the defect had no effect on superoxide anion production by normal PMNs. PMN rosetting with IgG-sensitized human erythrocytes was normal. Defective production of superoxide anion may contribute to the amelioration of connective tissue disease and increased susceptibility to infection often seen during pregnancy.
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives*; D009239:N-Formylmethionine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D011961:Receptors, Fc; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009842:Oligopeptides; D011961:Receptors, Fc; D013481:Superoxides; D009239:N-Formylmethionine; D011374:Progesterone; D004958:Estradiol; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen