title: Determination of short-circuit current in the in vivo perfused rat colon.
abstract: Current pulses (I) were injected into the lumen of proximal colonic segments in vivo, and the corresponding voltage deflections (delta PD) superimposed on the transcolonic PD were recorded. From the exponential decay of delta PD along the colon axis, the electrical length constant (lambda) was determined. Based on cable analysis the input resistance (= delta PD x = 0/I) and lambda made it possible to calculate the specific resistance (Rm) of the colonic epithelium as 128 +/- 16 omega X cm2. As Rm proved to be an ohmic resistor, the extrapolation from open-circuit PD (8-12 mV, lumen negative) to zero PD was feasible and made the calculation of short-circuit current (= PD/Rm) equal to 70 +/- 16 microA/cm2. In the presence of amiloride short-circuit current decreased to about 50%, whereas with theophylline it increased by about 30%. Substitution of luminal Na+ with choline or Cl- with cyclamate was associated with a marked increase of Rm. The rheogenic component of net Na+ transport was estimated to be only 8%. Electroneutral Na+ absorption functionally coupled with Cl- absorption displayed the characteristic feature of ion transport in the rat proximal colon.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002712:Chlorides / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000502:physiology*; D004553:Electric Conductivity; D004558:Electric Stimulation; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
chemical_list: D002712:Chlorides; D007473:Ion Channels; D012964:Sodium