Preparation of hormone-sensitive adipocytes from foetal rat brown adipose tissue.
A collagenase-dispersion method involving Percoll gradient separation is described for the production of adipocytes from foetal rat brown adipose tissue. The cells produced are hormone-sensitive as judged by cAMP accumulation and lipolysis.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000196:embryology / Q000502:physiology; D002001:Adipose Tissue, Brown / Q000166:cytology* / Q000196:embryology; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D008053:Lipid Mobilization / Q000187:drug effects; D003012:Microbial Collagenase; D051381:Rats
D000255:Adenosine Triphosphate; D005934:Glucagon; D000242:Cyclic AMP; D003012:Microbial Collagenase; D004837:Epinephrine