[New method for the study of the cation center of the Na,K-ATPase system].
An analysis of the shapes of the kinetic curves for the cationic sites of the Na,K-ATPase system revealed four completely activated Na-sites. Free ATP can change the so-called operation regime of Na,K-ATPase. At ATP excess two completely activated K-sites are detected, whereas at low ATP concentrations only one incompletely activated site is observed.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D002414:Cations, Monovalent; D007700:Kinetics; D008722:Methods; D011188:Potassium / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D002414:Cations, Monovalent; D000255:Adenosine Triphosphate; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium
Novyĭ metod analiza kationnykh tsentrov Na,K-ATPaznoĭ sistemy.