Isolation of horse mononuclear cells, especially of monocytes, on Isopaque-Ficoll neutral density gradient.
Horse mononuclear cells were separated from whole blood using neutral density gradient centrifugation on Isopaque-Ficoll. The resulting cell suspension was comparable in composition with similarly prepared human and bovine mononuclear cell preparations. The relative concentration of monocytes was increased by the use of a gradient with density lower than that originally proposed by Böyum (Böyum, A. 1968. Scand. J. Clin. Lab. Investig. 21 supple. 97:77-89). Contamination by neutrophils was limited either by using a gradient medium of lower density or by replacing Isopaque-Ficoll by Percoll-0.9% NaCl. Although the density of the Isopaque-Ficoll appears to be the main determinant in the isolation method of Böyum, the mechanism of separation of the cell population is complex and a substantial variability of the results can be expected.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D005362:Ficoll; D006736:Horses / Q000097:blood*; D008214:Lymphocytes; D008794:Metrizoic Acid; D009000:Monocytes; D009504:Neutrophils; D011205:Povidone; D012822:Silicon Dioxide; D012965:Sodium Chloride
D005362:Ficoll; D012965:Sodium Chloride; C016039:Percoll; D012822:Silicon Dioxide; D008794:Metrizoic Acid; D011205:Povidone