Preparation of ligation intermediates and related polynucleotide pyrophosphates.
Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives*; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular / Q000379:methods*; D011088:DNA Ligases / Q000378:metabolism; D004274:DNA, Recombinant; D011756:Diphosphates; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides
D004274:DNA, Recombinant; D011756:Diphosphates; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C010671:adenosine 5'-phosphoroimidazolide; D000249:Adenosine Monophosphate; D011088:DNA Ligases