Conditioned medium from a clonal Rous sarcoma virus transformed cerebellar cell line induces process extension in glial lines.
A Rous Sarcoma virus transformed cerebellar cell line, BC6, secretes a factor which causes clonal glial cell lines to rapidly (1 h) extend processes. The factor shows a degree of specificity since only 3 out of 10 lines which exhibit either glial or combined glial and neuronal properties respond. The active factor appears to be a soluble protein since it remains in the supernatant after centrifugation at 100,000 g for 2 h and is trypsin-sensitive. When conditioned medium is fractionated on a Sephadex G-100 column, activity elutes in and just behind the void volume. By several criteria the factor is distinct from glia maturation factor.
D000818:Animals; D001358:Avian Sarcoma Viruses; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D002531:Cerebellum / Q000378:metabolism*; D002999:Clone Cells; D006133:Growth Substances / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology*; D051381:Rats
D006133:Growth Substances