Viral and cellular fos proteins: a comparative analysis.
The FBJ murine osteosarcoma virus (FBJ-MuSV) induces osteosarcomas in mice and transforms fibroblasts in vitro. It contains an oncogene termed v-fos derived from a normal cellular gene by recombination with an associated helper virus. The product of the v-fos gene is a 55,000 dalton protein, p55v-fos. This protein was found in the nuclei of cells containing amplified levels of the v-fos gene, and also in the nuclei of virus-transformed cells. The c-fos protein was localized in the nuclei of normal mouse amnion cells and in the nuclei of cells transformed by a recombinant plasmid that expresses the c-fos gene product. However, p55c-fos undergoes more extensive post-translational modification in the nucleus than p55v-fos. Immunofluorescence data indicate that the level of p55c-fos in normal mouse amnion cells is similar to that found in fibroblasts transformed by the v-fos or c-fos proteins.
D000650:Amnion; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005260:Female; D005784:Gene Amplification; D005796:Genes; D005814:Genes, Viral; D051379:Mice; D009857:Oncogenes; D010053:Ovary; D010957:Plasmids; D011247:Pregnancy; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D004274:DNA, Recombinant; D014764:Viral Proteins; D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes