Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.
Gene fusions between the Escherichia coli lacZ gene and DNA segments containing the simian virus 40 early promoter or the mouse mammary tumor virus (MMTV) promoter direct the synthesis of functional beta-galactosidase in Cos 7 monkey cells and mouse Ltk-cells. Enzymatic activity was measured either 72 h after transfection or in stable transformants. The sensitive beta-galactosidase assay was used to measure gene expression and to optimize the efficiency of DNA-mediated transfection. Glucocorticoids stimulated the production of beta-galactosidase when lacZ was fused to the hormonally regulated MMTV promoter.
D004274:DNA, Recombinant; D003907:Dexamethasone / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D007763:Lac Operon; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
D004274:DNA, Recombinant; D003907:Dexamethasone; D005696:Galactosidases; D001616:beta-Galactosidase