Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.
A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D005260:Female; D006133:Growth Substances / Q000235:genetics*; D000882:Haplorhini; D007377:Interleukin-3; D008407:Mast Cells / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D014981:Xenopus
D006133:Growth Substances; D007377:Interleukin-3; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes