Metabolism of N-acylethanolamine phospholipids by a mammalian phosphodiesterase of the phospholipase D type.
Rat heart contains a membrane-bound phosphodiesterase of the phospholipase D type which catalyzes the hydrolysis of 1,2-diacyl-sn-glycero-3-phospho(N-acyl)ethanolamine to N-acylethanolamine and phosphatidic acid. The enzyme also hydrolyzes the corresponding alkenylacylglycerophospho(N-acyl)ethanolamine and N-acylethanolamine lysophospholipids but not phosphatidylcholine or phosphatidylethanolamine. The activity is highest in the microsomal fraction, does not require Ca2+ or Mg2+, and is stimulated by Triton X-100. Bile salts, other ionic detergents, and Zn2+ are inhibitory. Hydrolysis occurs over a wide pH range, with the activity at acid pH being stimulated by freezing and thawing. Other rat tissues also release N-acylethanolamine from N-acylethanolamine phospholipids.
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D017830:Octoxynol; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014018:Tissue Distribution; D015032:Zinc / Q000494:pharmacology
D001647:Bile Acids and Salts; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D017830:Octoxynol; D010740:Phospholipases; D010727:Phosphoric Diester Hydrolases; D010739:Phospholipase D; D008274:Magnesium; D015032:Zinc; D002118:Calcium