Cytoskeletal disarrangement in rat intestinal epithelium after in vivo exposure to secretagogues.
Cholera toxin (CT) and E. coli heat-labile enterotoxin (LT) induced hypersecretion in the small intestine, as did cytochalasin B and dibutyryl-cyclic AMP (DB-cAMP). The cytoskeleton in the apical part of the intestinal epithelial cells was disorganized after challenge with either of the four secretagogues, but not cholera B-subunit toxoid, as revealed by immunofluorescence microscopy using actin and the intermediate filament keratin as markers. Electron microscopic analysis confirmed the re-engagement of the terminal web and the appearance of short microvilli lacking most of their normally observed central core of actin filaments. Pretreatment with chloroquine prevented cytoskeletal disorganization as well as hypersecretion by CT, but not by cytochalasin B. A similar effect was achieved with chloroquine on CT-induced fluid secretion, while chlorpromazine inhibited the fluid response to cytochalasin B as well as to CT. The observed cytoskeletal re-engagement might be one of the reactions behind enterotoxic diarrhoea.
D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002738:Chloroquine / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D002772:Cholera Toxin / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004768:Enterotoxins / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D003571:Cytochalasin B; D003994:Bucladesine; D002738:Chloroquine; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli; D003078:Colchicine; D002746:Chlorpromazine