Synapsis promoted by Ustilago rec1 protein.
Ustilago rec 1 protein pairs homologous DNA molecules by promoting both synapsis and strand transfer. Complexes formed with rec 1 protein and a homologous combination of single-stranded and duplex DNA that appear to be synaptic structures can be detected by use of a nitrocellulose filter assay. The nascent heteroduplex formed during synapsis is a paranemic joint in which the single-stranded DNA pairs, but does not interwind, with its complement in the duplex molecule. Formation of the paranemic joint is accompanied by duplex unwinding and genesis of left-handed Z-DNA.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D004265:DNA Helicases / Q000502:physiology; D043211:Exodeoxyribonuclease V; D005656:Fungal Proteins / Q000502:physiology*; D008540:Meiosis; D009690:Nucleic Acid Conformation; D011995:Recombination, Genetic / Q000187:drug effects; D014588:Ustilago
D005656:Fungal Proteins; D000244:Adenosine Diphosphate; D043211:Exodeoxyribonuclease V; D004265:DNA Helicases