Iodide organification defect in a cold thyroid nodule: absence of iodide effect on cyclic AMP accumulation.
A follicular adenoma of the thyroid was 'hot' one hour after 99mTc pertechnetate administration, but 'cold' 24 h after 131I iodide administration. Incubation of the tissue in vitro demonstrated a defect in iodide binding to proteins that was abolished by addition of an H2O2 generating system. In this tissue iodide failed to inhibit TSH-induced cyclic AMP accumulation. The results show that iodide oxidation is required for its inhibitory action on cyclic AMP accumulation in human thyroid.
D000236:Adenoma / Q000000981:diagnostic imaging / Q000378:metabolism*; D000328:Adult; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008713:Methimazole / Q000494:pharmacology; D011193:Potassium Iodide / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D011877:Radionuclide Imaging; D013670:Sodium Pertechnetate Tc 99m; D013667:Technetium; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology
D007457:Iodine Radioisotopes; D011193:Potassium Iodide; D008713:Methimazole; D013667:Technetium; D013972:Thyrotropin; D013670:Sodium Pertechnetate Tc 99m; D000242:Cyclic AMP