Effect of inhibitors of protein synthesis on dopamine modulation of the slow-EPSP in rabbit superior cervical ganglion.
The protein synthesis inhibitors, anisomycin and cycloheximide, were tested for their ability to prevent dopamine-induced long-term enhancement of the slow-EPSP in rabbit superior cervical ganglion. Exposure of ganglia to either inhibitor of protein synthesis, at a concentration that suppressed [3H]leucine incorporation into ganglionic protein by at least 95%, had no effect on the development of dopamine-induced enhancement of the slow-EPSP. Incubation of ganglia with dopamine, without an inhibitor of protein synthesis, was without effect on [3H]leucine incorporation into ganglionic protein. It is concluded that synthesis of new protein is not required for the development of long-term enhancement of the slow-EPSP induced by dopamine.
D000818:Animals; D000841:Anisomycin / Q000008:administration & dosage; D003513:Cycloheximide / Q000008:administration & dosage; D004298:Dopamine / Q000008:administration & dosage / Q000494:pharmacology*; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011817:Rabbits; D009435:Synaptic Transmission / Q000187:drug effects*
D009419:Nerve Tissue Proteins; D000841:Anisomycin; D003513:Cycloheximide; D004298:Dopamine