Effects of GABA receptor agonists on [3H] flunitrazepam binding to rat cerebellar and hippocampal membranes.
The effects of GABA agonists on [3H] flunitrazepam binding were examined in membranes from CNS areas proposed to contain different populations of benzodiazepine binding site subtypes (BZ1/BZ2). Since these effects were broadly similar in both cerebellar and hippocampal membranes, it seems unlikely that GABA receptors interacting with the proposed BZ1/BZ2 binding sites have markedly different properties. Sodium chloride clearly facilitated the potentiating effect of muscimol on [3H] flunitrazepam binding but produced a complex interaction with the effects of GABA itself and the partial agonist imidazoleacetic acid in the phosphate buffer system used.
D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D005445:Flunitrazepam / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009118:Muscimol / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D007093:Imidazoles; D010880:Piperidines; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam; C023402:piperidine-4-sulfonic acid; C005954:imidazoleacetic acid