Glucoresponsive neurons in rat ventromedial hypothalamic tissue slices in vitro.
Extracellular action potentials were recorded from rat ventromedial hypothalamic nucleus (VMH) tissue slices in vitro. The identified glucoresponsive and non-glucoresponsive neurons were then visually located and observed by intracellular horseradish peroxidase staining. Glucoresponsive neurons, which were predominantly multipolar, were found near the center of the VMH, while non-glucoresponsive neurons, half of which were bipolar and half multipolar, were found randomly throughout the VMH.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D046508:Culture Techniques; D008564:Membrane Potentials; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology*; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology*
D001786:Blood Glucose; D011956:Receptors, Cell Surface; C027786:glucose receptor