Human rotavirus detection by counterimmunoelectrophoresis versus enzyme immunoassay and electron microscopy after direct ultracentrifugation.
The sensitivity of the counterimmunoelectrophoresis test with NCDV, Wa, and SA-11 rotavirus antisera was 60, 60, and 67%, respectively. The counterimmunoelectrophoresis specificity was greater than 99%, but the low sensitivity is a limiting feature of this test as a first-line immunodiagnostic test for rotavirus detection.
D003378:Counterimmunoelectrophoresis; D006801:Humans; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D012401:Rotavirus / Q000302:isolation & purification*; D014461:Ultracentrifugation