The spontaneous activation of a potassium channel during the preparation of resealed human erythrocyte ghosts.
Resealed erythrocyte ghosts prepared under conditions which deplete the cell of its endogenous chelators and metabolites are found to be selectively permeable to potassium. The net efflux of potassium is stimulated by low concentrations of external potassium and can be inhibited by oligomycin. The effect is not expressed when resealed ghosts are formed by hemolysis in the presence of chelators or magnesium. The spontaneously activated pathway is actually the calcium-activated potassium channel, first discovered by Gardos in 1958. In the intact cell, the combined actions of the calcium pump and endogenous chelators maintain the calcium concentration below the threshold for activation. Current observations indicate that the channel is spontaneously activated by traces of calcium originating from the cell itself or from the unavoidable background of calcium found in the media. The channel in ghosts depleted of endogenous chelators exhibits its high affinity for calcium. Channel activation occurs during hemolysis and persists throughout subsequent washings.
D002118:Calcium / Q000097:blood; D002413:Cations, Divalent; D004910:Erythrocyte Membrane / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D009840:Oligomycins / Q000494:pharmacology; D011188:Potassium / Q000097:blood*
D002413:Cations, Divalent; D007473:Ion Channels; D009840:Oligomycins; D011188:Potassium; D002118:Calcium