Inactivation of non-A, non-B virus infectivity by a beta propiolactone/ultraviolet irradiation treatment and Aerosil adsorption procedure used for preparation of a stabilized human serum.
Inoculation of chimpanzees revealed that starting material for the preparation of a pooled stabilized human serum preparation was contaminated with non-A, non-B hepatitis virus(es). This material was subjected to beta-propiolactone/ultraviolet irradiation treatment and adsorption with an insolubilized silicic acid under conditions employed for sterilization of the final product (Biseko, Biotest). The treated material did not transmit hepatitis to 2 inoculated chimpanzees. These animals were subsequently found to be susceptible to non-A, non-B infection by challenge with the untreated material. These findings demonstrate a non-A, non-B type hepatitis virus to be susceptible to inactivation by the methods employed for sterilization of this stabilized human serum preparation.
D000327:Adsorption; D000818:Animals; D005260:Female; D006509:Hepatitis B / Q000209:etiology / Q000517:prevention & control; D006526:Hepatitis C / Q000209:etiology / Q000517:prevention & control*; D006518:Hepatitis Viruses / Q000254:growth & development*; D006524:Hepatitis, Viral, Animal / Q000209:etiology / Q000517:prevention & control; D006525:Hepatitis, Viral, Human / Q000517:prevention & control*; D006801:Humans; D007783:Lactones / Q000627:therapeutic use*; D008297:Male; D002679:Pan troglodytes; D011420:Propiolactone / Q000627:therapeutic use*; D012824:Silicic Acid / Q000627:therapeutic use*; D012822:Silicon Dioxide / Q000627:therapeutic use*; D013242:Sterilization; D065227:Transfusion Reaction; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects / Q000528:radiation effects
D007783:Lactones; D012824:Silicic Acid; D011420:Propiolactone; D012822:Silicon Dioxide