Synaptic interactions in the GABA system during postnatal development in retina.
Using biochemical analyses, we have demonstrated the presence of a high-affinity, sodium- and temperature-dependent uptake system for GABA in the retinas of newborn rabbits. The activity of this system two days after birth is approximately 70 percent of adult values, slowly increasing to adult level by postnatal day 6-8. An intraocular injection nipecotic acid (final concentration = 10 mM) into one-day-old rabbit pups resulted in a 60 percent inhibition in uptake activity. In order to study the possible role of the GABA uptake system in retinal development, we have determined the consequences of blocking GABA uptake with nipecotic acid on the postnatal development of post-synaptic GABA receptors, as measured by 3H-muscimol binding. Nipecotic acid treatment caused a significant increase in receptor binding in retinas prior to eye opening, with the maximal stimulation being one day after the intraocular injection. Our data indicate that the development of GABA receptor sites is influenced by the activity of the GABA uptake system and suggest that GABA may function as a trophic factor in the developing rabbit retina.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D009118:Muscimol / Q000378:metabolism; D009557:Nipecotic Acids / Q000494:pharmacology; D011392:Proline / Q000031:analogs & derivatives*; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism; D011963:Receptors, GABA-A; D012160:Retina / Q000166:cytology*; D009435:Synaptic Transmission / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D009557:Nipecotic Acids; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; C030278:nipecotic acid; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D011392:Proline; C010983:homoproline