title: Nucleotide sequence of the lysozyme gene of bacteriophage T4. Analysis of mutations involving repeated sequences.
abstract: The nucleotide sequence of the lysozyme (e) gene of bacteriophage T4 and approximately 130 additional nucleotides on each side has been determined. The 5'-end of the gene for internal protein III appears to be located about 70 base-pairs from the 3'-end of the lysozyme gene. Nucleotide sequence analysis of mutant e genes confirmed that three identified hotspots of frameshift mutations are runs of five A nucleotides in the wild-type gene. The endpoints of two deletions are direct repeats of eight base-pairs in the wild-type gene. Two frameshift mutations with high reversion frequencies are duplications of five or seven base-pairs. The cloning and nucleotide sequence determination of the lysozyme gene will facilitate further study of the molecular biology of T4 lysozyme.
mesh_terms: D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D009113:Muramidase / Q000235:genetics*; D009154:Mutation; D012091:Repetitive Sequences, Nucleic Acid; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D003062:Codon; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D009113:Muramidase