Effects of deletions in transposon Tn7 on its frequency of transposition.
Deletions in transposon Tn7 either abolished transposition or reduced transposition frequency. Except for a deletion in the right-hand terminus, these deletions could be complemented in trans. A 2.1-kilobase fragment of Tn7 encodes a diffusible gene product which stimulates transposition above the wild-type frequency. No cointegrate formation was detected.
D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D009713:Nucleotidyltransferases / Q000235:genetics; D010957:Plasmids; D019895:Transposases
D004251:DNA Transposable Elements; D009713:Nucleotidyltransferases; D019895:Transposases; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII