Solubilization of angiotensin II receptors in rat glomeruli.
125I-labelled angiotensin II (A II) specifically binds to solubilized receptors extracted from rat isolated glomeruli using CHAPS (3-[3-( cholamidopropyl ) dimethylammonio ]-1-propanesulfonate). The yield of solubilization of the binding sites was 3.3%. Equilibrium was reached after 15-20 min and specific binding represented 75% of total binding. Dissociation of the hormone-receptor complex after addition of an excess of A II was very slow in the presence of Ca2+ and Mg2+. [Sar1 Ala8] A II and [Sar1 Ile8] A II were more potent as competitive inhibitors of 125I-labelled A II than A II itself and its heptapeptide. These basic features of 125I-labelled A II binding to the extracted material were similar to those observed previously with untreated glomeruli.
D015059:1-Sarcosine-8-Isoleucine Angiotensin II / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002793:Cholic Acids; D003902:Detergents; D007678:Kidney Glomerulus / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000302:isolation & purification* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D012504:Saralasin / Q000378:metabolism; D012995:Solubility
D002793:Cholic Acids; D003902:Detergents; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D000804:Angiotensin II; D015059:1-Sarcosine-8-Isoleucine Angiotensin II; D012504:Saralasin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate