Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface.
The cell surface receptor for CSF-1 has been identified by affinity labeling intact mouse bone marrow-derived macrophages and intact cells of the murine J774.2 and BAC1 lines, which differ in their specific CSF-1 binding capacities and biological responses. The receptor, labeled by crosslinking to 125I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D003432:Cross-Linking Reagents; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000378:metabolism; D010586:Phagocytes / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D016184:Receptors, Colony-Stimulating Factor; D013388:Succinimides / Q000378:metabolism
D000345:Affinity Labels; D003115:Colony-Stimulating Factors; D003432:Cross-Linking Reagents; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor; D013388:Succinimides; C019358:disuccinimidyl suberate