Histamine and serotonin suppression of lymphocyte response to phytohemagglutinin and allogeneic cells.
Histamine added to murine spleen cells suppressed in vitro proliferation of lymphocytes induced by PHA or allogeneic spleen cells. Another vasoactive amine, serotonin (5-hydroxytryptamine), exerted a similar inhibitory activity on PHA- or allogeneic cell-induced lymphocyte proliferation. Anti-H2 histamine antagonists, cimetidine, metiamide, and ranitidine, blocked the histamine and serotonin suppressive effect. Cyproheptadine, an anti-H1 histamine and anti-serotonin antagonist, and methysergide, an anti-serotonin antagonist, also blocked histamine and serotonin inhibitory activities. These data suggest the presence, on lymphocytes, of receptors for serotonin which might be related to histamine receptors.
D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D017259:Dimaprit; D004336:Drug Antagonism; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008785:Metiamide / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011899:Ranitidine / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology*; D013890:Thiourea / Q000494:pharmacology
D010835:Phytohemagglutinins; D012701:Serotonin; D008785:Metiamide; D002927:Cimetidine; D006632:Histamine; D011899:Ranitidine; D013890:Thiourea; D017259:Dimaprit