Phospholipase A2 modulation by calmodulin, prostaglandins and cyclic nucleotides.
Phospholipase A2 in the presence of Ca2+ was stimulated by calmodulin and by prostaglandin F2 alpha. Prostaglandin E2, cyclic-AMP and cyclic-GMP inhibited phospholipase A2 in the presence or absence of calmodulin. Dimethylsuberimidate cross-linking of phospholipase A2 with calmodulin was found to be Ca2+ dependent. These results indicate that phospholipase A2 is directly regulated by a host of key intracellular regulators and is one of the calmodulin-regulated enzymes.
D000818:Animals; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D004120:Dimethyl Suberimidate / Q000494:pharmacology; D015237:Dinoprost; D015232:Dinoprostone; D004546:Elapid Venoms; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D004546:Elapid Venoms; D046911:Macromolecular Substances; D011458:Prostaglandins E; D011460:Prostaglandins F; D004120:Dimethyl Suberimidate; D015237:Dinoprost; D000242:Cyclic AMP; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D006152:Cyclic GMP; D015232:Dinoprostone