Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
We report the fate of different derivatives of the vitellogenin genes after injection into fertilized eggs of Xenopus. We injected a constructed minigene as well as a 5' fragment of the A2 vitellogenin gene. The minigene survives in embryogenesis much better than the 5' A2 fragment and is retained more frequently and at a higher level in frog tissues. The mosaic distribution of the foreign DNA in different frog tissues indicates that no integration occurred before the first cleavage stage. The persisting DNA may be partially integrated but is mostly found in an episome-like form. This unintegrated form is not supercoiled and is rearranged. Methylation of the Hpa II sites prior to injection has no influence on the survival of the injected sequences and the Hpa II sites of the surviving DNA are unmethylated irrespective whether the injected DNA was methylated or not. Whereas the derivatives are transcribed in embryos, they cannot be activated by estrogen in the liver of young frogs.
D000818:Animals; D001483:Base Sequence; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005260:Female; D005306:Fertilization; D005796:Genes; D008074:Lipoproteins / Q000235:genetics*; D008675:Metamorphosis, Biological; D008745:Methylation; D009030:Mosaicism; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism*; D010060:Ovulation / Q000187:drug effects; D014158:Transcription, Genetic; D014819:Vitellogenins / Q000235:genetics*; D014981:Xenopus
D006063:Chorionic Gonadotropin; D008074:Lipoproteins; D014819:Vitellogenins; D004247:DNA; D004262:DNA Restriction Enzymes