Comparison of the DNA sequence of the Crawford small-plaque variant of polyomavirus with those of polyomaviruses A2 and strain 3.
The DNA sequence of two wild-type strains of polyomavirus (A2 and strain 3) are known. We have determined the majority of the DNA sequence of a third strain, the Crawford small-plaque virus. This virus has been noted for its capacity to induce readily detected tumor-specific transplantation antigen in hamster cells, a property that is most likely attributable to an altered middle T-antigen. A comparison of its DNA sequence with those of the A2 and strain 3 viruses reveals numerous nucleotide substitutions, insertions, and deletions throughout the genome. Most sequence changes in coding regions are silent mutations; however, variability in proteins can be predicted from these sequence data at 5 locations in middle T-antigen, 10 in large T-antigen, and 10 in VP1. The Crawford small-plaque virus noncoding regulatory region contains, in addition to nucleotide substitutions, a 44-base-pair tandem repeat of sequences on the late side of the origin of DNA replication.
D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004279:DNA, Viral; D005814:Genes, Viral; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics* / Q000254:growth & development; D012091:Repetitive Sequences, Nucleic Acid; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins; D014779:Virus Replication
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins