[Effects of cianidanol (KB-53) on experimental liver injury in mice--histopathological, histochemical and enzyme-histochemical studies].
Protective effects of KB-53 on acute liver injury induced by carbon tetrachloride (CCl4) and 1-naphtylisothiocyanate (ANIT) in mice was investigated by means of histopathological, histochemical and enzymehistochemical examinations. Diffuse centrilobular necrosis, ballooning degeneration of hepatocytes and hemorrhage were markedly observed in the livers of the mice one to three days after a subcutaneous injection of CCl4. On the other hand, in the livers of KB-53 pretreated mice, forcal necrosis was observed and inflammatory cells had already infiltrated one day after CCl4-intoxication. Three days later, remarkable development of absorbent granulation tissues with syncytium and hepatocytic mitosis was observed. Furthermore, a number of PAS positive materials such as mucopolysaccharides and mucoproteins were detected in the livers of KB-53 pretreated mice. KB-53 inhibited the disappearance of glucose-6-phosphatase (G-6-Pase) in the liver after CCl4-intoxication and the rise of alkaline phosphatase (Al-Pase) in the liver after ANIT-intoxication. In addition, KB-53 inhibited the rise of Al-Pase and total bilirubin in the serum of mice after CCl4 and ANIT-intoxication. All these findings suggest that KB-53 protects liver against the morphological and functional changes, and it potentiates the proliferative and regenerative activity of the liver impaired with CCl4 and ANIT.
D015058:1-Naphthylisothiocyanate / Q000633:toxicity; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D002392:Catechin / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury; D005419:Flavonoids / Q000494:pharmacology*; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006651:Histocytochemistry; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008107:Liver Diseases / Q000201:enzymology / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D001578:Benzopyrans; D005419:Flavonoids; D015058:1-Naphthylisothiocyanate; D002392:Catechin; D002251:Carbon Tetrachloride; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase