title: A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A.
abstract: Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage lambda BamHI vector. Recombinant phage (lambda KOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D018139:Simplexvirus / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes