The distinction of the white pulp from the red pulp in guinea pig spleen by the use of sequential histoenzymatic methods.
Using the sequential method for revealing of NADH-cytochrome c reductase and other enzymes (acid and alkaline phosphatases, non-specific esterase, peroxidase) or cationic proteins in the same section, the distinct borders between white and red pulp of the guinea pig spleen were delineated. The sequential method enables not only these areas of the spleen to be clearly distinguished, but also the migration of granulocytes from the red to the white pulp. The implications of these findings are discussed in terms of spleen function.
D015720:5'-Nucleotidase; D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D006098:Granulocytes / Q000201:enzymology*; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods; D008214:Lymphocytes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D009245:NADH Dehydrogenase / Q000032:analysis; D009283:Naphthol AS D Esterase / Q000032:analysis; D009708:Nucleotidases / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D013154:Spleen / Q000166:cytology*
D010544:Peroxidases; D009245:NADH Dehydrogenase; D009283:Naphthol AS D Esterase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D015720:5'-Nucleotidase