title: Influence of streptozotocin- and alloxan-induced diabetes in the rat on collagenase and certain lysosomal enzymes in relation to the degradation of connective tissue proteins.
abstract: The activity of collagenase and certain lysosomal hydrolases (cathepsin B1, cathepsin D, beta-glucuronidase and beta-N-acetyl glucosaminidase) was studied in serum and tissues of rats with streptozotocin- or alloxan-induced diabetes. The activity of serum lysosomal enzymes was increased in both groups (p less than 0.05). Both streptozotocin- and alloxan-diabetic animals showed significantly higher dermal collagenase activity than those of controls (p less than 0.01), but the liver and spleen showed similar activities; there was a significant decrease in the renal collagenase activity of streptozotocin-diabetic rats (p less than 0.05). Comparison of the alloxan- or streptozotocin-treated groups with control animals showed an increase in lysosomal enzymes (cathepsin B1, cathepsin D, beta-glucuronidase and beta-N-acetyl glucosaminidase in skin, liver and spleen) (p less than 0.05) but beta-N-acetyl glucosaminidase was unchanged in the spleen of streptozotocin-diabetic rats. There was no difference in renal cathepsin B1 and D in control versus alloxan-diabetic rats, but there was an increase in beta-glucuronidase and beta-N-acetyl glucosaminidase (p less than 0.05). The streptozotocin-diabetic animals showed decreased activities of renal lysosomal enzymes (p less than 0.05), but similar activity of cathepsin D to the control animals.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D002401:Cathepsin B; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism*; D003238:Connective Tissue / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D005966:Glucuronidase / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000201:enzymology; D013154:Spleen / Q000201:enzymology
chemical_list: D006596:Hexosaminidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D002403:Cathepsins; D002401:Cathepsin B; D002402:Cathepsin D; D003012:Microbial Collagenase