The parental origin of X chromosomes in XX males determined using restriction fragment length polymorphisms.
The inheritance of several X-linked restriction fragment length polymorphisms ( RFLPs ) is examined in seven 46,XX males and their immediate relatives. The XX males are shown to have inherited a paternal and a maternal RFLP allele in each of the five (of seven) families in which these X-linked markers are informative. In the other two families, a maternal X-chromosomal contribution is demonstrated, but a paternal contribution cannot be determined. We conclude that most, if not all, XX males inherit one paternal and one maternal X chromosome. A segment of single-copy DNA specific to the short arm of the Y chromosome is found to be absent from the genomes of eight XX males. In one of these XX males, an Xp-Yp translocation had previously been inferred from chromosome-banding studies. Our findings argue against mosaicism involving a Y-containing cell line in the XX males examined here, but they do not exclude an X-Y (or Y-autosome) translocation during paternal meiosis. If such a translocation has occurred, the translocation product received by the XX males does not include the Yp-specific sequence tested here.
D000483:Alleles; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D007713:Klinefelter Syndrome / Q000235:genetics*; D007962:Leukocytes / Q000648:ultrastructure; D008297:Male; D008540:Meiosis; D009030:Mosaicism; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic; D014960:X Chromosome / Q000032:analysis*; D014998:Y Chromosome / Q000032:analysis
D004247:DNA; D004262:DNA Restriction Enzymes