Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function.
A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.
D001482:Base Composition; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D009876:Operon; D013329:Structure-Activity Relationship; D013604:T-Phages / Q000201:enzymology; D014158:Transcription, Genetic
D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes