Poly(ADP-ribosylation) of a DNA topoisomerase.
A DNA topoisomerase activity, copurifying with poly(ADP-ribose) synthetase from calf thymus, is greater than 95% inhibited if extensive poly(ADP-ribosylation) is allowed to occur. The inhibited DNA topoisomerase, which has drastically different elution properties on hydroxylapatite, can be reactivated by mild alkaline treatment. These results are consistent with a poly(ADP-ribosylation) of the DNA topoisomerase and covalent attachment of the poly(ADP-ribose) moieties to the topoisomerase by alkali-labile bonds.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D002850:Chromatography, Gel; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification / Q000378:metabolism*; D017886:Durapatite; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D013950:Thymus Gland / Q000201:enzymology; D059004:Topoisomerase I Inhibitors
D006882:Hydroxyapatites; D009702:Nucleoside Diphosphate Sugars; D059004:Topoisomerase I Inhibitors; D011064:Poly Adenosine Diphosphate Ribose; D017886:Durapatite; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase; D004264:DNA Topoisomerases, Type I