title: Two physically and serologically distinct lipopolysaccharide profiles in strains of Bordetella pertussis and their phenotype variants.
abstract: The lipopolysaccharide (LPS) from nine strains representing 18 phenotype variants of Bordetella pertussis could be grouped into one of two distinct profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. One group, representing the wild-type LPS profile of B. pertussis, consisted of two silver-staining bands: a dominant brown-amber a band and a faster-migrating, minor, black-staining b band. The second group, representing a variant LPS profile, consisted of a single black-staining band of similar mobility to the b band in the wild-type profile. By electrophoretic transfer (Western) blot analysis, mouse antiserum raised against whole cells of Tohama I (prototype wild-type LPS strain) recognized only the a band from all strains/phenotypes possessing the wild-type LPS profile. In contrast, mouse antiserum raised against whole cells of 134 (prototype variant LPS strain) recognized all b bands, regardless of strain/phenotype, and could be shown to cross-react weakly with the a band from Tohama I. These results and results from cohemagglutination and immunodiffusion analyses support the classification of B. pertussis into one of two physiologically and serologically distinct LPS phenotypes: Lps AB for the wild-type profile and Lps B for the variant profile. The relationship of LPS type and phenotypic, or "phase," variation is discussed.
mesh_terms: D000818:Animals; D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D012703:Serotyping; D013045:Species Specificity
chemical_list: D008070:Lipopolysaccharides