mRNA discrimination in extracts from uninfected and reovirus-infected L-cells.
Uncapped reovirus mRNA extracted at late times from infected L-cells is preferentially translated in extracts from infected L-cells. However, translation of this uncapped, late, reovirus mRNA in extracts from infected cells is sensitive to inhibition by the cap analog m7GTP . These results imply that reovirus infection does not induce a transition from cap-dependent to cap-independent translation. Nevertheless, the results of in vitro translational competition experiments between L-cell mRNA and late viral mRNA were consistent with the view that reovirus does induce an alteration in the cap-dependent translational apparatus of L-cells. The reduced efficiency of translation of a variety of capped mRNAs in extracts from infected cells is also consistent with this notion. We further conclude that a factor exists in reovirus-infected L-cells that specifically stimulates translation of uncapped reovirus mRNAs.
D000818:Animals; D002460:Cell Line; D005347:Fibroblasts; D012089:Mammalian orthoreovirus 3 / Q000235:genetics*; D051379:Mice; D014176:Protein Biosynthesis / Q000187:drug effects; D012314:RNA Cap Analogs / Q000494:pharmacology; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012087:Reoviridae / Q000235:genetics*; D012088:Reoviridae Infections / Q000235:genetics*
D012314:RNA Cap Analogs; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; C017516:7-methylguanosine triphosphate