title: Immunological characterization of an Escherichia coli strain which is lacking cytochrome d.
abstract: The isolated membranes from an Escherichia coli mutant strain which lacks spectroscopically detectable levels of cytochromes d, a1, and b558 also have abnormally low levels of N,N,N',N'-tetramethyl-p-phenylenediamine oxidase activity. In this paper, it is shown that the material previously identified as the N,N,N',N'-tetramethyl-p-phenylenediamine oxidase is, in fact, the two-subunit cytochrome d complex. Antisera directed against the native cytochrome d complex as well as against each of two subunits apparent on sodium dodecyl sulfate-polyacrylamide gels were used to show that the mutant strain lacks both subunits of the cytochrome d complex. Introduction of F-prime F152 into the mutant strain restored the two subunits along with the spectroscopic and enzymatic activity associated with the cytochrome d complex.
mesh_terms: D003378:Counterimmunoelectrophoresis; D017937:Cytochrome d Group; D003580:Cytochromes / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005144:F Factor; D009154:Mutation; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism
chemical_list: D003580:Cytochromes; D017937:Cytochrome d Group; D010089:Oxidoreductases, N-Demethylating; C022726:tetramethylphenylenediamine oxidase