Specificity of a cytochemical bioassay for arginine-vasopressin and its validation for plasma measurement.
The total Na+/K+ ATP-ase activity of the thick ascending limb of the loop of Henle may be stimulated by arginine-vasopressin (AVP). Lysine-vasopressin (LVP), oxytocin (OT), and arginine-vasotocin (AVT) produce less than 5% of the enzyme activity induced by the same concentration of AVP. Physiological concentrations of a mixture of other hormones with known activity on the kidney (T3, T4, aldosterone, angiotensin II, and OT) did not significantly increase total Na+/K+ ATP-ase activity. Specific AVP antiserum consistently removed greater than 90% of the stimulatory effect of plasma. The concentration of AVP in plasmas from dehydrated subjects was greater than 10 times that of the same subjects hydrated. Intra-assay coefficient of variation was 35% and 52% from 200 microliters and 20 microliters of plasma respectively. The interassay coefficient of variation was 53% and 55% from plasma pools with high and low AVP content.
D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood* / Q000276:immunology; D001681:Biological Assay; D003681:Dehydration / Q000097:blood; D006651:Histocytochemistry; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007684:Kidney Tubules / Q000201:enzymology*; D008138:Loop of Henle / Q000201:enzymology*; D008236:Lypressin / Q000494:pharmacology; D010121:Oxytocin / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014668:Vasotocin / Q000494:pharmacology
D007106:Immune Sera; D001127:Arginine Vasopressin; D010121:Oxytocin; D008236:Lypressin; D000254:Sodium-Potassium-Exchanging ATPase; D014668:Vasotocin