Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.
A modified phosphotriester method has been employed for the efficient chemical synthesis of long-chain deoxyribooligonucleotides. During the course of this work, a general and rapid procedure was developed for the preparation of 24-62-mers in solution. Preparative reversed phase column chromatography on silanized silica gel was used to purify triester intermediates starting from 10-mers. The rapid synthesis of 32-mer and 42-mer on glass and silica gel supports using suitably protected 2-8-mer blocks as coupling units has been also accomplished. In particular, a convenient procedure for the solid-phase synthesis of oligonucleotide blocks bearing 3'-terminal phosphodiester groups is described.
D000266:Adenylyl Imidodiphosphate / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D005898:Glass; D007093:Imidazoles; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D012822:Silicon Dioxide; D013329:Structure-Activity Relationship
D007093:Imidazoles; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D000266:Adenylyl Imidodiphosphate; D012822:Silicon Dioxide; C018100:1-methylimidazole