EGF receptor binding studies in endometrial cell culture.
Guinea pig endometrial cells were isolated and maintained in cell culture. Our investigation of these cultures for the presence of epidermal growth factor (EGF) binding activity demonstrated that these cells possess EGF receptors. Upon the addition of EGF to growing cell cultures, an increase in the rate of cell growth resulted along with a higher saturation density. The binding of EGF to these cells is saturable at 40-50 ng/ml., and the receptors demonstrate "down-regulation" in response to hormonal challenge at 37 degrees C.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D011374:Progesterone / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*
D003470:Culture Media; D011956:Receptors, Cell Surface; D011374:Progesterone; D004958:Estradiol; D004815:Epidermal Growth Factor; D066246:ErbB Receptors