Adrenocorticotropin: ACTH1-38 is a major product of biotransformation by brain synaptic membranes.
Since adrenocorticotropic hormone is found in the brain, and several of its fragments affect adaptive behavior, the formation of fragments of ACTH1-39 by a rat brain synaptic membrane fraction was investigated. Following the incubations at physiological pH conditions, the digests were fractionated by HPLC to quantitate the amounts of ACTH1-39 remaining and products formed. Time- and enzyme-dependent disappearance of ACTH1-39 was accompanied by the accumulation of a major peptide metabolite (product B). Amino acid analysis and NH2-terminal end-group determination revealed that product B was identical to ACTH1-38. These results indicate the predominance of carboxypeptidase activity in the degradation of ACTH1-39 by brain synaptic membranes.
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis* / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D001921:Brain / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010446:Peptide Fragments / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D013570:Synaptic Membranes / Q000378:metabolism*
C027550:ACTH (1-38); D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone