A problem in the radiochemical assay of glucose-6-phosphatase in muscle.
A radiochemical assay for glucose-6-phosphatase, which depends on the absorption of radiolabelled substrate on to a nascent precipitate of BaSO4 and Zn(OH)2, can give rise to erroneously high activities. Adsorption on to a Fe(OH)3 precipitate does not expose the incubation medium to extreme alkaline conditions and provides a satisfactory and simple assay. This method demonstrates a very low activity of the enzyme in mammalian muscle, in contrast with previous findings.
D000818:Animals; D005290:Ferric Compounds; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D008297:Male; D008722:Methods; D009132:Muscles / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011854:Radiochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D005290:Ferric Compounds; C021024:ferric hydroxide; D010744:Phosphoric Monoester Hydrolases; C040842:glycerol-1-phosphatase; D005952:Glucose-6-Phosphatase