[Behavior of Mycoplasma in a continuous cell culture of embryonic sheep kidney producing the bovine leukemia virus].
Mycoplasma sp. previously isolated from sheep embryo kidney cells producing bovine leukemia virus (FLK-BLV) was serologically identified in the growth inhibition test as M. arginini. SDS-PAGE of cellular proteins demonstrated their identity in M. sp. and M. arginini. Upon artificial inoculation of FLK cells, M. arginini persisted in the cells for a long time and was detectable in high titres by all the methods used. Other species of mycoplasma were isolated from the cells by microbiological method but were detectable by other methods in first passages only. The phenomenon of "disappearance" of mycoplasma was not associated with the toxic effect on them of the ingredients used in cell propagation and of cell metabolism products. It is assumed that sheep embryo cells, M. arginini, and bovine leukemia virus is a fortunate biological model of natural infection.
D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney / Q000382:microbiology*; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification*; D009174:Mycoplasma / Q000145:classification / Q000302:isolation & purification*; D012190:Retroviridae / Q000302:isolation & purification*; D012703:Serotyping; D012756:Sheep; D013997:Time Factors
Povedenie mikoplazm v kul'ture perevivaemykh kletok pochki émbriona ovtsy, produtsiruiushchikh virus leĭkoza krupnogo rogatogo skota.