Gene transfer by polyoma-like particles assembled in a cell-free system.
Empty capsids of polyoma virus interact with DNA in a cell-free system to form polyoma-like particles (PLP). The DNA in these particles is protected from the action of pancreatic deoxyribonuclease. Transfer of genetic information by PLP has been accomplished by using a restriction fragment containing the transforming sequences of polyoma DNA as a model gene. Infection of rat F111 cells by PLP containing these sequences results in DNA-mediated cellular transformation. Gene transfer by PLP is 50 to 150 times more efficient than by the calcium phosphate precipitation method.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D005814:Genes, Viral; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats
D004279:DNA, Viral; D004247:DNA