Ecto-5'-nucleotidase activities in B lymphoblastoid cell lines at various stages of maturation.
B lymphoblastoid cell lines (LCL) at various stages of maturation, including possible precursor B cell lines without immunoglobulin (Ig) expression, pre-B cell-like LCL, and Ig-secreting LCL, were established from peripheral and bone marrow mononuclear cells of four patients with congenital agammaglobulinemia (CAG) by infection with Epstein-Barr virus (EBV) and estimated for their ecto-5'-nucleotidase (ecto-5'-NT, E.C. 3.1.3.5) activity. Ecto-5'-NT activities in Ig-secreting LCL derived from CAG patients fell into the same range of those in LCL derived from control subjects (from 7.3 to 33.0 nmoles/hr/10(6) cells). When ecto-5'-NT activities of precursor B cell lines derived from the CAG patients were also compared with Ig-secreting LCL derived from control subjects, no significant difference in ecto-5'-NT activities was found among LCL with various Ig expression which might indicate various stages of B cell maturation. These results may suggest that ecto-5'-NT does not appear to be a biochemical marker of B cell maturation.
D015720:5'-Nucleotidase; D000328:Adult; D000361:Agammaglobulinemia / Q000097:blood / Q000151:congenital*; D001402:B-Lymphocytes / Q000201:enzymology*; D001853:Bone Marrow / Q000473:pathology; D002454:Cell Differentiation; D002460:Cell Line; D004854:Herpesvirus 4, Human; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008099:Liver / Q000196:embryology; D009708:Nucleotidases / Q000378:metabolism*
D007136:Immunoglobulins; D009708:Nucleotidases; D015720:5'-Nucleotidase