title: The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.
abstract: The nucleotide sequence of 1100bp around the origin of replication of the pSC101 plasmid has been determined. This segment of DNA is capable of replication in the presence of a helper plasmid. The sequence data reveal similarities between pSC101 and several other replicons. The origin of replication contains three direct repeats of an 18bp sequence associated with a segment exceptionally rich in A-T base pairs. A promotor that probably directs transcription of a gene encoding an essential plasmid replication function is associated with a region of extensive potential secondary structure. The sequence presented here includes the sequence of the par region involved in partitioning of plasmids at cell division.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D009876:Operon; D010957:Plasmids
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes