Chemiluminescence of asbestos-activated macrophages.
Chemiluminescence, a measure of reactive oxygen release by phagocytes, was compared in peritoneal exudate macrophages elicited with chrysotile asbestos, Corynebacterium parvum and saline. Chrysotile asbestos- and C. parvum-activated macrophages produced significantly more chemiluminescence than saline-elicited macrophages. In a second series of experiments the ability of opsonized chrysotile asbestos to act as a trigger for the release of chemiluminescence was tested. Opsonized chrysotile asbestos produced a dose-related release of chemiluminescence from activated macrophages except at the highest dose where chemiluminescence was reduced due, possibly, to a toxic effect of chrysotile during the assay. Opsonized latex also triggered a dose-related chemiluminescent response from activated macrophages. The potential role of toxic reactive oxygen species, released from macrophages, in the development of asbestos-related pulmonary inflammation and fibrosis are discussed.
D000818:Animals; D001194:Asbestos / Q000494:pharmacology*; D017632:Asbestos, Serpentine; D001202:Ascitic Fluid; D002374:Catalase / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007840:Latex; D008163:Luminescent Measurements; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009504:Neutrophils / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects; D011425:Propionibacterium acnes; D013482:Superoxide Dismutase / Q000494:pharmacology
D017632:Asbestos, Serpentine; D007840:Latex; D001194:Asbestos; D002374:Catalase; D013482:Superoxide Dismutase