Cell surface receptors for ecotropic murine viruses are different from those for xenotropic and amphotropic viruses.
The murine cell surface receptor for Rauscher murine leukemia virus was analyzed using a binding assay involving cultured murine cells and 125I-labeled envelope glycoprotein, gp70, of the virus. The binding was competitively inhibited by extracts of ecotropic murine viruses. No inhibition was observed with a xenotropic or an amphotropic murine type-C virus, a murine type-B virus, and several nonmurine retroviruses. Unlike cells producing ecotropic murine viruses, murine cell lines actively producing either an endogenous xenotropic virus or an amphotropic virus showed a significant degree of receptor activity for Rauscher murine leukemia virus gp70. When murine cells were induced with iododeoxyuridine to produce a xenotropic virus and an ecotropic virus in distinct phases, the receptor activity of the induced cells remained unchanged during xenotropic virus release, but a dramatic decrease was evident with the onset of ecotropic virus release. These findings show that ecotropic murine viruses share a common receptor on the murine cell surface and that this receptor is distinct from those for xenotropic and amphotropic murine viruses.
D000818:Animals; D001667:Binding, Competitive; D017988:Gammaretrovirus / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000382:microbiology; D011925:Rauscher Virus / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D012190:Retroviridae / Q000378:metabolism; D013045:Species Specificity; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism; D014775:Virus Activation
D011991:Receptors, Virus; D014759:Viral Envelope Proteins; D014764:Viral Proteins