title: Non-opiate analgesia induced by carbachol microinjection into the pontine parabrachial region of the cat.
abstract: These studies investigated the effect of microinjection of the cholinergic agonist carbamylcholine (carbachol) into various sites of the dorsolateral pontine tegmentum of the cat. Carbachol microinjection into an area surrounding the lateral half of the brachium conjunctivum (parabrachial region, PBR) produced profound suppression of nociceptive responses. In the dorsal part of PBR, carbachol microinjection produced no generalized sensory, emotional or motor deficits, indicating that nociceptive transmission was primarily affected. Carbachol microinjection into the ventral part of PBR resulted in slight suppression of motor responses in addition to profound nociceptive suppression. Carbachol-produced analgesia (CPA) observed within PBR blocked supraspinally as well as spinally integrated responses normally elicited by either phasic or tonic noxious stimuli. Atropine sulfate, but not mecamylamine hydrochloride, significantly antagonized CPA, indicating that muscarinic receptors mediate this phenomenon. The opiate antagonist naloxone, systemically administered either prior to or after carbachol microinjection, did not reliably attenuate CPA. Microinjection of morphine into the sites from which CPA had previously been obtained did not produce significant effects on nociceptive responses. Thus, opiate mechanisms appear not to be necessary either for the activation of this system or for the production of the resultant analgesia. These findings indicate that the neural population examined in the present study is anatomically and pharmacologically distinct from previously identified opiate-mediated pain inhibitory systems. Results are discussed in light of other recent evidence indicating the existence of endogenous non-opiate pain inhibitory systems.
mesh_terms: D000818:Animals; D001143:Arousal / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology*; D002415:Cats; D008465:Mechanoreceptors / Q000187:drug effects; D008636:Mesencephalon / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology; D009433:Neural Inhibition / Q000187:drug effects; D009434:Neural Pathways / Q000187:drug effects; D009619:Nociceptors / Q000187:drug effects*; D011149:Pons / Q000187:drug effects*; D011950:Receptors, Cholinergic / Q000187:drug effects*; D012018:Reflex / Q000187:drug effects; D013116:Spinal Cord / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D011950:Receptors, Cholinergic; D009270:Naloxone; D002217:Carbachol