title: A spin label study on the effect of dibutyryl cyclic AMP on synaptic plasma membranes isolated from rat brain.
abstract: The fluidity of the deep layer of the lipid bilayer and the mobility of sulphydryl (-SH) groups of synaptic plasma membranes, isolated from rat brain and spin-labelled with a doxyl derivative of stearic acid and with a nitroxide derivative of maleimide, respectively, were studied using electron spin resonance (ESR) techniques. Dibutyryl cyclic AMP (db-cAMP) and adenosine triphosphate (ATP) had no effect on the fluidity of the deep layer of the lipid bilayer of synaptic plasma membranes. While protein dephosphorylation was inhibited by Zn2+, db-cAMP together with ATP increased the mobility of -SH groups above the value obtained with db-cAMP alone.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000378:metabolism*
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D003994:Bucladesine; D000255:Adenosine Triphosphate