title: recF-dependent induction of recA synthesis by coumermycin, a specific inhibitor of the B subunit of DNA gyrase.
abstract: Genetic and biochemical studies on enzymes known to be involved in regulating DNA supercoiling yield a complex spectrum of effects on the Escherichia coli SOS system. Previous studies indicated that only inhibition of DNA gyrase by antibiotics that act on the DNA gyrase A subunit results in turning on the E. coli SOS system. Here we show that coumermycin, an antibiotic that acts on the DNA gyrase B subunit, can also induce. Like nalidixic acid induction, coumermycin induction is dependent on the recBC DNase. In both cases induction apparently results from a response of the cell to the DNA gyrase-inhibitor complex rather than just the loss of DNA gyrase activity. However, unlike induction by the DNA gyrase A-specific antibiotics, coumermycin induction also requires the recF gene product. This demonstrates a functional relationship between DNA gyrase and the recF gene product.
mesh_terms: D049933:Aminocoumarins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D003374:Coumarins / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D004278:DNA, Superhelical / Q000096:biosynthesis; D003851:Deoxyribonucleases / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D046911:Macromolecular Substances; D011938:Rec A Recombinases; D059005:Topoisomerase II Inhibitors
chemical_list: D049933:Aminocoumarins; D001426:Bacterial Proteins; D003374:Coumarins; D004278:DNA, Superhelical; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D059005:Topoisomerase II Inhibitors; D011938:Rec A Recombinases; D003851:Deoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; C004628:coumermycin