Hormone-dependent enhancement of neoplastic phenotype expression by MuMTV-infected heterologous cells, and MuMTV-induced cellular antigens.
A new property, i.e. acquisition of insulin (insulin + dexamethasone)-mediated enhancement of anchorage independence by heterologous MuMTV-infected cells, is described. Hormone-mediated anchorage-independent growth enhancement in MuMTV-infected cells is followed by expression of a cell-surface antigen, recognized by allogeneic (C3H----AKR) anti-Thy 1.2 antigen serum. The absorption test and the use of monoclonal antibody to the Thy 1.2 antigen showed that hormone-dependent antigen on the cell surfaces of experimentally and naturally MTV-infected cells was different from the Thy 1.2 antigen itself. Utilization of anti-p53 ("protein of transformation") monoclonal antibody enabled us to identify this cell membrane antigen as p53.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D002448:Cell Adhesion; D002460:Cell Line; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D003907:Dexamethasone / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D007328:Insulin / Q000494:pharmacology*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010641:Phenotype; D014410:Tumor Stem Cell Assay; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000956:Antigens, Viral; D003470:Culture Media; D007328:Insulin; D014764:Viral Proteins; D003907:Dexamethasone