The association of amyloid P-component (AP) with the amyloid fibril: an updated method for amyloid fibril protein isolation.
An amyloid fibril isolation procedure is proposed which uses citrate as well as saline washes to dissociate the calcium dependent linkage of amyloid P-component (AP) from the amyloid fibril. In two amyloid rich tissues, the amount of AP was quantitated in each saline and citrate wash and totalled 13.8% and 20.8% of the amyloid fibrils isolated. The amount of AP removed from these and 22 additional amyloid rich tissues was greater than had previously been recognized. AP protein was present in tissue only when amyloid fibrils were present. It could not be found in normal non-amyloidotic tissue, nor could it be found in tissue sediment after the fibrils were removed.
D000682:Amyloid / Q000302:isolation & purification*; D002118:Calcium; D002614:Chelating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D002951:Citrates; D019343:Citric Acid; D006801:Humans; D007122:Immunoelectrophoresis; D008099:Liver / Q000032:analysis; D003012:Microbial Collagenase; D000683:Serum Amyloid P-Component; D013154:Spleen / Q000032:analysis
D000682:Amyloid; D002614:Chelating Agents; D002951:Citrates; D000683:Serum Amyloid P-Component; D019343:Citric Acid; D003012:Microbial Collagenase; D002118:Calcium