The similarity between tuftsin (Thr-Lys-Pro-Arg) receptors and tuftsin antibody: a case of induced molecular mimicry.
The characteristics of binding of tuftsin (Thr-Lys-Pro-Arg) and several of its synthetic analogues, to specific membrane receptors on rabbit polymorphonuclear granulocytes, were compared with the binding characteristics of rabbit specific antibody to tuftsin. [3H-Arg4]-tuftsin was used as the principal ligand. Six analogues were studied. Two of these, Thr-Lys-Pro and Ala-Lys-tuftsin-Glu-Ala3, showed no binding affinity either to receptor or antibody. Ala-Lys-tuftsin showed less binding than tuftsin to both acceptors. Three showed stronger binding than tuftsin. The order of binding among these was tuftsin ( [Glu]2-tuftsin ( [Pro-Pro3]-tuftsin (tuftsinyltuftsin. This same order of binding was found with both receptor and antibody.
D000818:Animals; D000906:Antibodies / Q000502:physiology*; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011971:Receptors, Immunologic; D013329:Structure-Activity Relationship; D014405:Tuftsin / Q000031:analogs & derivatives / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D009842:Oligopeptides; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C029695:tuftsin receptor; C033480:tuftsinyltuftsin; D014405:Tuftsin