Comparison of pyrimidine 5'nucleotidase activity in erythrocytes of sheep, dogs, cats, horses, calves, and Mongolian gerbils.
Pyrimidine 5'nucleotidase (P5N) activities of erythrocytes for Mongolian gerbils, cats, dogs, sheep, horses, and calves were measured, using a radiometric technique with [14C]cytidine monophosphate as the substrate. Erythrocytes of gerbils had the highest activity [1,177.1 +/- 133.6 mU/g of hemoglobin (Hb)]. Feline erythrocytes had 327.4 +/- 204.4 mU/g of Hb. Canine erythrocytes had 148.0 +/- 19.8 mU/g of Hb. Ovine erythrocytes (44.3 +/- 20.9 mU/g of Hb), equine erythrocytes (30.0 +/- 15.9 mU/g of Hb), and bovine erythrocytes (14.1 +/- 6.9) had relatively low P5N activity. The P5N activity was approximately proportional to the reticulocyte percentage of and inversely proportional to the mean erythrocyte life span in these species.
D015720:5'-Nucleotidase; D000818:Animals; D002415:Cats / Q000097:blood*; D002417:Cattle / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004905:Erythrocyte Aging; D004906:Erythrocyte Count / Q000662:veterinary; D004912:Erythrocytes / Q000201:enzymology*; D005849:Gerbillinae / Q000097:blood*; D006736:Horses / Q000097:blood*; D009708:Nucleotidases / Q000097:blood*; D012156:Reticulocytes / Q000166:cytology; D012756:Sheep / Q000097:blood*; D013045:Species Specificity
D009708:Nucleotidases; D015720:5'-Nucleotidase