Sites of single-strand breaks in DNA treated with a diol-epoxide of benz[a]anthracene.
Evidence presented here indicates that r-8,t-9-dihydroxy-t-10,11-oxy-8,9,10,11-tetrahydrobenz[a]anthracene can introduce single-strand breaks into DNA. The positions of the single-strand breaks were determined in experiments in which the diol-epoxide was incubated with a defined 85 base pair DNA molecule that was radiolabelled at one 3' end with 32P. Analysis of the radiolabelled DNA fragments produced in these experiments in DNA sequencing gels showed that the breaks occur at most but not all guanines and at some adenines.
D001482:Base Composition; D001483:Base Sequence; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D008854:Microscopy, Electron; D009153:Mutagens / Q000633:toxicity*; D013539:Simian virus 40
D001551:Benz(a)Anthracenes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009153:Mutagens; C026622:8,9-dihydro-8,9-dihydroxybenzanthracene 10,11-oxide