title: The effects of ionophores and metabolic inhibitors on methanogenesis and energy-related properties of Methanobacterium bryantii.
abstract: The effects of numerous ionophores and inhibitors were tested on methane synthesis, intracellular ATP and potassium concentrations, and the proton motive force of the methanogenic archaebacterium Methanobacterium bryantii. M. bryantii had an internal pH near 6.8 (and hence little delta pH during growth) with an electrical potential of --127 mV in growth medium and --105 mV in a pH 6.5 buffer. The study has identified agents which, in M. bryantii, can effectively cause a decline of intracellular ATP (gramicidin, acetylene) and potassium concentrations (gramicidin, nigericin), inhibit methane synthesis (acetylene, gramicidin, nigericin, triphenylmethylphosphonium bromide), eliminate the electrical potential (high extracellular potassium ion concentrations), and dissipate artificially imposed, inside alkaline, pH gradients (monensin, nigericin, carbonyl cyanide m-chlorophenylhydrazone). Carbonyl cyanide m-chlorophenylhydrazone was generally ineffective in media or buffers reduced with cysteine-sulfide but could be effective in cysteine-free solutions reduced with hydrogen sulfide.
mesh_terms: D000255:Adenosine Triphosphate / Q000302:isolation & purification; D004734:Energy Metabolism / Q000187:drug effects*; D019605:Euryarchaeota / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007476:Ionophores / Q000494:pharmacology*; D008697:Methane / Q000096:biosynthesis*; D011188:Potassium / Q000302:isolation & purification; D011522:Protons
chemical_list: D007476:Ionophores; D011522:Protons; D000255:Adenosine Triphosphate; D008697:Methane; D011188:Potassium