title: Physical and functional map of the hemolytic plasmid pSU316.
abstract: A restriction endonuclease analysis of the hemolytic plasmid pSU316 has allowed location of the cleavage sites for the endonucleases BamHI, XbaI, KpnI, Bg/II, Sa/GI, EcoRI, and HindIII. Hybridization experiments between pSU316 and pED100 have shown that the tra region of pSU316 lies in a segment comprising part of Sa/GI fragments S-1 and S-3 and the entire fragment S-4. The positions of other plasmids coded functions, namely the replication functions and alpha-hemolysin production, have been determined in the physical map.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D010957:Plasmids; D012093:Replicon
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D004262:DNA Restriction Enzymes