Adjuvant effects of a crystalline silica on IgE and IgG1 antibody production in mice and their prevention by the macrophage stabilizer poly-2-vinylpyridine N-oxide.
A crystalline silica (standard quartz DQ12 with particle size less than 5 microns) is able to stimulate in Balb/c mice the production of IgE and IgG1 antibody to a single 1-microgram dose of ovalbumin. The adjuvant effects of silica on both IgE and IgG1 antibody production are prevented by pretreatment of animals with poly-2-vinylpyridine N-oxide, a polymer that protects macrophages from the well-documented toxic effects of silica. These results indicate that adjuvanticity of silica is, at least partly, correlated to the damage induced on macrophages.
D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage; D011144:Polyvinylpyridine N-Oxide / Q000494:pharmacology*; D011145:Polyvinyls / Q000494:pharmacology*; D011791:Quartz / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D011145:Polyvinyls; D011791:Quartz; D007073:Immunoglobulin E; D012822:Silicon Dioxide; D010047:Ovalbumin; D011144:Polyvinylpyridine N-Oxide