Biochemical genetics of the mouse IgM system.
Using a mouse hybridoma system, we have developed methods of isolating a variety of mutant cell lines in which immunoglobulin function or synthesis is defective. The analysis of mutants defective in kappa chain synthesis has defined a class of murine transposons. The deletion mutants produce immunoglobulin M (IgM) bearing mu heavy chain fragments and provide information on the requirements of IgM assembly and mu gene expression. We also describe a transfer system for the mu and kappa genes which will be useful in analyzing the structural basis of IgM function.
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements; D005784:Gene Amplification; D006825:Hybridomas; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009154:Mutation; D010957:Plasmids; D014161:Transduction, Genetic; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D007075:Immunoglobulin M; D004247:DNA