Activation of oxidative and arachidonic acid metabolism in neutrophils by respiratory syncytial virus antibody complexes: possible role in disease.
The effect of respiratory syncytial virus (RSV) antibody complexes on the metabolism of human neutrophils was determined by examining the generation of luminol-dependent chemiluminescence, superoxide, and thromboxane B2. Incubation of neutrophils with RSV antibody complexes resulted in a significant increase in the production of chemiluminescence. The increase in chemiluminescence appeared to be due to (1) active phagocytosis of RSV antibody complexes as evidenced by 70% inhibition with cytochalasin B (P less than 0.001) or (2) increased superoxide production as evidenced by 40% inhibition with superoxide dismutase (P less than 0.001). The generation of superoxide was confirmed by specific analysis in a superoxide dismutase-inhibitable ferricytochrome c reduction assay. Of particular importance was the observation that RSV antibody complexes induced the release of significant quantities of thromboxane B2 from neutrophils as determined by radioimmunoassay. Oxygen radicals and/or products of arachidonic acid metabolism may, in part, mediate the pathogenesis of RSV infection through direct tissue damage and bronchoconstriction.
D000328:Adult; D000914:Antibodies, Viral / Q000502:physiology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001990:Bronchiolitis, Viral / Q000209:etiology / Q000276:immunology; D003571:Cytochalasin B / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000378:metabolism*; D012136:Respiratory Syncytial Viruses / Q000276:immunology; D010253:Respirovirus Infections / Q000150:complications / Q000276:immunology*; D013481:Superoxides / Q000096:biosynthesis; D013929:Thromboxane B2 / Q000096:biosynthesis
D000914:Antibodies, Viral; D001095:Arachidonic Acids; D013481:Superoxides; D016718:Arachidonic Acid; D003571:Cytochalasin B; D013929:Thromboxane B2