Promutagen activation with mammalian and avian S9 liver microsomes.
Metabolic activation activity of Aroclor 1254 induced rat liver microsomes (S9) were compared with uninduced microsomes obtained from beef, pig, sheep and chicken liver. Using the Ames Salmonella typhimurium mutagenesis assay (strains TA98 and TA100), in conjunction with benzo(a)pyrene and 2-aminoanthracene, the highest promutagen activation levels were obtained with chicken liver S9. The S9 from the other domestic animals gave results which were close to those of the induced rat S9 values. Enhanced promutagen activation activity was observed with chicken S9 following freeze drying and reconstitution of the S9 preparation.
D000818:Animals; D000873:Anthracenes / Q000378:metabolism*; D001140:Aroclors / Q000494:pharmacology; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism*; D001711:Biotransformation; D002417:Cattle; D002645:Chickens; D020111:Chlorodiphenyl (54% Chlorine); D008099:Liver / Q000187:drug effects*; D008110:Liver Extracts / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D051381:Rats; D012486:Salmonella typhimurium; D012756:Sheep; D013045:Species Specificity
D000873:Anthracenes; D001140:Aroclors; D001580:Benzopyrenes; D008110:Liver Extracts; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine); D001564:Benzo(a)pyrene; C006593:2-anthramine