Joining of V kappa to J kappa gene segments in a retroviral vector introduced into lymphoid cells.
V kappa to J kappa recombination occurred within a DNA construct introduced into cells in the form of a defective retrovirus. Analysis of one recombinant demonstrated that V kappa-J kappa joining can occur via inversion with a net loss of a few base pairs.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007144:Immunoglobulin J-Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D004262:DNA Restriction Enzymes