Detergent solubilisation of rat liver particulate cyclic AMP phosphodiesterase.
Detergent solubilisation of particulate rat liver low Km cyclic AMP phosphodiesterase in the presence of protease inhibitors yields a form of the enzyme with a larger molecular weight than the form solubilised by protease treatment. The detergent solubilised enzyme could be partially purified by anion exchange chromatography. It displayed a marked tendency to precipitate from solution when detergent was removed.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000302:isolation & purification*; D000818:Animals; D002845:Chromatography / Q000379:methods; D003902:Detergents; D008099:Liver / Q000201:enzymology*; D011092:Polyethylene Glycols; D051381:Rats; D012995:Solubility
D003902:Detergents; D011092:Polyethylene Glycols; D015105:3',5'-Cyclic-AMP Phosphodiesterases