title: Quantification of bovine IgG, IgM and IgA antibodies to Clostridium perfringens B-toxin by enzyme immunoassay I. Preparturient immunization for enhancement of passive transfer of immunity.
abstract: A quantitative competitive binding "triple-sandwich" enzyme immunoassay was developed and used to evaluated pathogen/class-specific antibody responses in Holstein-Friesian cows vaccinated against Clostridium perfringens B-toxin. Vaccination of cows at six weeks and again at two weeks prepartum increased pathogen-specific IgG levels in each dam's colostrum and respective calf's serum. Pathogen-specific IgG and IgM concentrations in dams' sera and colostra were related to subsequent pathogen-specific IgG and IgM neonatal sera concentrations. Only pathogen-specific IgA in dams' colostra was correlated to neonatal levels, possibly owing to a different origin and role of this immunoglobulin class. All class-specific colostral immunoglobulin levels were related to subsequent neonatal concentrations. Isotypic antibody responses against C. perfringens B-toxin were found with pathogen-specific IgM predominant in dams' sera and pathogen-specific IgA predominant in colostra and neonatal sera.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000917:Antibody Formation; D002417:Cattle; D003016:Clostridium perfringens / Q000276:immunology*; D005260:Female; D007116:Immunization, Passive; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000145:classification*; D007074:Immunoglobulin G / Q000145:classification*; D007075:Immunoglobulin M / Q000145:classification*; D011247:Pregnancy
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M