Metabolic interconversion of dolichol and dolichyl phosphate during development of the sea urchin embryo.
The in vivo and in vitro synthesis and turnover of dolichol and dolichyl phosphate have been studied over the course of early development in sea urchin embryos. Synthesis of dolichol and dolichyl phosphate was studied in vivo and in vitro using [3H]acetate and [14C] isopentenylpyrophosphate, respectively, as precursors. Both the in vivo and in vitro results indicate that the principal labeled end product of de novo synthesis is the free alcohol, and that this alcohol is subsequently phosphorylated to produce dolichyl phosphate. The presence of 30 microM compactin inhibits the de novo synthesis of dolichol from [3H]acetate by greater than 90%, but has no effect on the incorporation of 32Pi into dolichyl phosphate for more than 6 h, thus suggesting that during this time interval the major source of dolichyl phosphate is preformed dolichol. The rate of turnover of the [3H]acetate-labeled polyisoprenoid backbone of dolichyl phosphate is very slow (t1/2 = 40-70 h). In contrast, the rate of loss of the [32P]phosphate headgroup is more rapid (t1/2 = 5.7-7.7 h) and increases over the course of development. Finally, dolichyl phosphate phosphatase activity has been measured in vitro. The activity of this enzyme, which can be distinguished from phosphatidic acid phosphatase, was found to increase as a function of development, in qualitative agreement with the increased turnover of 32P from dolichyl phosphate observed in vivo. These results suggest that the phosphate moiety of dolichyl phosphate is in a dynamic state, and that dolichol kinase and dolichyl phosphate phosphatase play key roles in regulating the cellular level of dolichyl phosphate.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D004224:Diterpenes / Q000378:metabolism*; D004288:Dolichol Phosphates / Q000378:metabolism*; D004286:Dolichols / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology; D010711:Phosphatidate Phosphatase / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D012617:Sea Urchins / Q000196:embryology*
D000085:Acetates; D004224:Diterpenes; D004288:Dolichol Phosphates; D004286:Dolichols; D011106:Polyisoprenyl Phosphates; C028306:dolichol monophosphate; D010744:Phosphoric Monoester Hydrolases; D010711:Phosphatidate Phosphatase; C020088:dolichyl-phosphatase; D005459:Fluorides