title: Phosphorylation of smooth muscle myosin light chain by five different kinases.
abstract: Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen synthase (casein) kinase-1) both serine and threonine. Isoelectric focusing analyses of 32P-labelled chymotryptic peptides indicate that phosphorylase kinase and cAMP-dependent protein kinase phosphorylate the same site as myosin light chain kinase. However, both casein kinase-2 and glycogen synthase (casein) kinase-1 phosphorylate different sites.
mesh_terms: D000818:Animals; D047388:Casein Kinases; D000242:Cyclic AMP / Q000378:metabolism; D007525:Isoelectric Focusing; D009130:Muscle, Smooth / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase; D009218:Myosins / Q000378:metabolism*; D010764:Phosphorylase Kinase / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D014422:Turkeys
chemical_list: D000242:Cyclic AMP; D011494:Protein Kinases; D010764:Phosphorylase Kinase; D047388:Casein Kinases; D009219:Myosin-Light-Chain Kinase; D009218:Myosins