title: Regeneration and protein synthesis in the spinal cord: the influence of ACTH-like peptides on cell-free protein synthesis.
abstract: To measure protein synthesis at the level of the cell bodies of motoneurons of the sciatic nerve, anterior horns were punched out of the spinal cord using microdissection techniques and a cell-free system was prepared. Injections of ACTH1-16 and ACTH4-10 were shown to enhance protein synthesis in the cell-free system, 1.5 h after treatment. Neither crush lesion of the sciatic nerve nor systemic treatment with ACTH4-10 produced clear changes in overall protein synthesis. Thus, the increase in overall protein synthesis by acute treatment with ACTH-like peptides cannot explain the previously reported stimulation of axonal regeneration after nerve crush.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D012584:Sciatic Nerve / Q000502:physiology; D013116:Spinal Cord / Q000378:metabolism / Q000502:physiology*
chemical_list: D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C039322:ACTH (1-16); D000324:Adrenocorticotropic Hormone; C011394:ACTH (4-10)