Molecular mechanism of uncoupling in brown adipose tissue mitochondria. The non-identity of proton and chloride conducting pathways.
Specific permeability properties of the inner membrane of brown adipose tissue mitochondria were analysed with the aid of simultaneous pH measurements outside mitochondria and of mitochondria swelling. It was shown that valinomycin-induced potassium diffusion potential drives a parallel passive uptake of chlorides and extrusion of proton. Electrogenic H+ -extrusion was independent on anion transport, no competition was found between the two processes and the former process exerted a lower sensitivity to the inhibitory effect of GDP. The existence of two distinct, independent pathways for translocation of protons and halide anions across the membrane is suggested.
D002001:Adipose Tissue, Brown / Q000648:ultrastructure*; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D006224:Cricetinae; D006153:Guanosine Diphosphate / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism*; D008647:Mesocricetus; D008928:Mitochondria / Q000378:metabolism*; D008933:Mitochondrial Swelling; D009550:Nigericin / Q000494:pharmacology; D011522:Protons; D014634:Valinomycin / Q000494:pharmacology
D002712:Chlorides; D007473:Ion Channels; D011522:Protons; D006153:Guanosine Diphosphate; D014634:Valinomycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D009550:Nigericin