Biphasic effects of muramyl dipeptide or lipopolysaccharide on superoxide anion-generating activities of macrophages.
The superoxide anion (O2-)-generating activity of guinea pig macrophages stimulated by wheat germ agglutinin (WGA), immune complexes, or phorbol myristate acetate (PMA) was studied after short- and long-term exposures of the cells to muramyl dipeptide (MDP) or lipopolysaccharide (LPS). Neither MDP nor LPS alone induced O2- release in macrophages. Short-term (30 min) exposure to these agents caused the enhanced release of O2- in response to WGA or immune complexes, though the PMA-induced O2- generation was not affected. On the other hand, long-term exposure (more than 24 h) to MDP or LPS progressively enhanced O2- generation of the cells induced by WGA, immune complexes, or even PMA. These results suggest that the mechanism for O2- generation of macrophages stimulated by WGA or immune complexes differs from that stimulated by PMA and that the differences also exist between short- and long-term exposure to MDP or LPS.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000818:Animals; D000936:Antigen-Antibody Complex; D006168:Guinea Pigs; D007700:Kinetics; D037102:Lectins; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014909:Wheat Germ Agglutinins
D000936:Antigen-Antibody Complex; D037102:Lectins; D008070:Lipopolysaccharides; D014909:Wheat Germ Agglutinins; D013481:Superoxides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate