A hepatitis B surface antigen polypeptide (P31) with the receptor for polymerized human as well as chimpanzee albumins.
Hepatitis B virus, and hepatitis B surface antigen particles co-occurring with hepatitis B e antigen, have the receptor for polymerized human and chimpanzee albumins that may be involved in the hepatotropism of hepatitis B virus. We identified the receptor on a hepatitis B surface antigen polypeptide with a molecular size of 31,000 daltons (P31). P31 bound to polymerized albumins from human and chimpanzee, but did not react with polymerized albumins from the experimental animals without susceptibility to hepatitis B virus. P31 was composed of the major polypeptide of hepatitis B surface antigen with a molecular size of approximately 22,000 daltons (P22) and additional 55 amino acid residues coded by the pre-S region in the hepatitis B virus-specific deoxyribonucleic acid. Because P22 did not react with polymerized albumin, the sequence of 55 amino acid residues in the pre-S region appeared to bear the receptor. Polypeptides with the receptor for polymerized albumin may make an efficacious hepatitis B vaccine, because they could raise antibodies against the putative site on hepatitis B virus capable of binding with hepatocytes to initiate the infection.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004198:Disease Susceptibility; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D002679:Pan troglodytes; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D010455:Peptides / Q000302:isolation & purification*; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D012709:Serum Albumin / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000596:Amino Acids; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D010446:Peptide Fragments; D010455:Peptides; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D014765:Viral Vaccines