[Immunization with antigen D. Results obtained with 118 volunteers].
During the last four years, 118 blood donors have been immunized to obtain plasma with a high level anti-D in order to prepare anti-D immunoglobulins. The results of the immunizing schedule are very successful, as we have obtained anti-D of titer superior to 256 in 96,36% of the cases (Coombs technic). However, the development of unwanted antibodies outside the Rh system (anti-Jka: 6, anti-Fya: 5) has led us since November 1979 to use phenotyped blood without undesirable red blood cell antigens. No irregular antibody has developed since except for an anti-Yta. The anti-HLA have been observed with a frequency of 36%. The use of frozen/thawed and phenotyped blood without undesirable red blood cell antigens can allow to obtain a high level of anti-D without risk for the donors. Nevertheless, the exceptional immunization to a public antigen persists.
D000328:Adult; D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000032:analysis; D001782:Blood Donors; D001789:Blood Group Antigens / Q000276:immunology; D001787:Blood Group Incompatibility / Q000209:etiology; D001803:Blood Transfusion; D017707:Erythrocyte Transfusion; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis*; D007518:Isoantibodies / Q000096:biosynthesis; D008297:Male; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D018029:Rho(D) Immune Globulin
D000961:Antilymphocyte Serum; D001789:Blood Group Antigens; D006680:HLA Antigens; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin
L'immunisation à l'antigène D. Résultats obtenus chez 118 volontaires.