Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.
Transfection of embryo fibroblasts by a human ras oncogene does not convert them into tumour cells unless the fibroblasts are established and immortalized before transfection. The embryo fibroblasts become tumorigenic if a second oncogene such as a viral or cellular myc gene or the gene for the polyoma large-T antigen is introduced together with the ras gene.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005816:Genetic Complementation Test; D051379:Mice; D009857:Oncogenes; D010641:Phenotype; D011120:Polyomavirus / Q000235:genetics
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor