Preparation of specific antisera to estrone-3-glucuronide and estriol-3-glucuronide.
The preparation and antigenic properties of estrone-3-glucuronide- and estriol-3-glucuronide-bovine serum albumin conjugates in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed high specificity to estrone-3-glucuronide and estriol-3-glucuronide, respectively, exhibiting little cross-reactivities with other estrogen conjugates and no cross-reactions with related steroids except for free estrogens, their 3-methyl ethers and 3-sulfates. The cross-reactive antibodies were eliminated by partial immunoadsorption on affinity chromatographic media using the estrone-3-methyl ether 17-(O-carboxymethyl)oxime- and estriol-3-methyl ether 16 (or 17)-hemisuccinate-aminohexyl Sepharose conjugates, respectively. The purified antisera exhibited no cross-reactivities with free estrogens and ring A conjugates of estrone and estriol.
D000818:Animals; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D004964:Estriol / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis; D004966:Estrogens, Conjugated (USP) / Q000032:analysis*; D004970:Estrone / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis; D006241:Haptens; D007106:Immune Sera; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D014316:Tritium
D000936:Antigen-Antibody Complex; D004966:Estrogens, Conjugated (USP); D006241:Haptens; D007106:Immune Sera; D007202:Indicators and Reagents; D014316:Tritium; C015856:estrone-3-glucuronide; C022810:estriol 3-glucuronide; D012710:Serum Albumin, Bovine; D004970:Estrone; D004964:Estriol