DNA binding properties of a mutant T antigen from the simian virus 40-transformed human cell line simian virus 80.
We investigated whether the T antigen of the simian virus 40-transformed human cell line simian virus 80 ( SV80 ) specifically recognizes DNA sequences of its own template, i.e, the viral sequences integrated in the SV80 cellular genome. In vitro DNA binding experiments clearly indicated that, in contrast to wild-type T antigen, SV80 T antigen does not specifically bind to sites on the integrated viral DNA in SV80 cells.
D000952:Antigens, Polyomavirus Transforming; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D010957:Plasmids; D011494:Protein Kinases / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D011494:Protein Kinases; D004262:DNA Restriction Enzymes