title: Replication of non-respiratory viruses in tracheal organ cultures.
abstract: Employing Hoorn's technique, tracheal explant cultures were set from adult hamsters, rabbits and human foetuses. To determine the susceptibility of these cultures they were infected with nine different mainly non-respiratory viruses. Assay of virus was carried out in susceptible cell lines. The results of these studies indicated that herpes simplex type I (HSV-1) and vaccinia viruses multiplied in these cultures and caused ciliostasis. Herpes simplex type 2 (HSV-2) although multiplying in all, produced ciliostasis only in human foetal tracheal cultures. However, Chandipura (CHP), West Nile (WN), sandfly fever (SF-N) and polio-1 viruses multiplied without ciliostasis. These cultures did not support multiplication of Japanese encephalitis (JE) and Chikungunya (CHIK) viruses. The studies indicated that mammalian tracheal cultures support replication of the non-respiratory viruses. The continued and undiminished multiplication of viruses over long periods without ciliostasis suggests a role for the trachea in the transmission of viral infections by aerosol.
mesh_terms: D000818:Animals; D006224:Cricetinae; D005333:Fetus; D006801:Humans; D008647:Mesocricetus; D009924:Organ Culture Techniques; D011817:Rabbits; D012209:Rhabdoviridae / Q000502:physiology; D018139:Simplexvirus / Q000502:physiology; D014035:Togaviridae / Q000502:physiology; D014132:Trachea / Q000196:embryology / Q000382:microbiology*; D014616:Vaccinia virus / Q000502:physiology; D014779:Virus Replication