Blocked herpes simplex virus type 2-specific DNA synthesis in simian virus 40-transformed hamster cells permissive for herpes simplex virus type 1.
Simian virus 40-transformed hamster cells (LL-1) permissive to herpes simplex virus type 1 (HSV-1) were shown to be relatively nonpermissive to HSV-2. When LL-1 cells were infected with HSV-2, there was a 3- to 4-log reduction in infectious viral progeny at 24 h postinfection as compared with HSV-1 under identical cultured conditions. HSV-2 could be carried in the LL-1 cell line for up to 12 passages without any appreciable cytopathology. Various early functions of the replicative cycle of HSV-2 appeared to be normal. Experiments demonstrated that early enzyme activity, HSV-2 thymidine kinase, and DNA polymerase appeared at permissive levels in extracts of HSV-2-infected LL-1 cells. However, DNA analysis of HSV-2 infected LL-1 cells demonstrated a block in HSV-2-specific DNA synthesis, although HSV-2 was capable of inhibiting DNA synthesis in LL-1 cells. Furthermore, indirect immunofluorescence studies indicate that late HSV-2 structural protein synthesis was inhibited in infected LL-1 cells. Thus, the inability of HSV-2 to replicate in LL-1 cells is due to a block at or before HSV-specific DNA synthesis, resulting in a reduction of the structural protein synthesis required for viral maturation.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000032:analysis; D008647:Mesocricetus; D010770:Phosphotransferases / Q000032:analysis; D013539:Simian virus 40 / Q000378:metabolism*; D018139:Simplexvirus / Q000378:metabolism*; D013937:Thymidine Kinase / Q000032:analysis; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation
D004279:DNA, Viral; D014764:Viral Proteins; D010770:Phosphotransferases; D013937:Thymidine Kinase; D004259:DNA-Directed DNA Polymerase