Possible exposure of leucine aminopeptidase on the cell surface of rabbit blood neutrophils by digitonin treatment.
The sensitivity of leucine aminopeptidase to diazotized sulfanilic acid (DSA) was compared between neutrophils from blood and peritoneal exudates of rabbit. The leucine aminopeptidase activity of peritoneal neutrophils was inhibited about 40% by DSA, whereas that of blood neutrophils was not inhibited at all by the reagent. However, pretreatment of blood neutrophils with digitonin in the presence and in the absence of divalent cations rendered leucine aminopeptidase sensitive to DSA to the same extent as peritoneal neutrophils, without affecting the cell viability and lactate dehydrogenase activity. These findings seem to indicate that the leucine aminopeptidase of blood neutrophils, which is normally inaccessible to DSA, was exposed on the cell surface by digitonin treatment.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology; D004072:Digitonin / Q000494:pharmacology*; D005372:Filipin / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007931:Leucyl Aminopeptidase / Q000097:blood*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000201:enzymology* / Q000378:metabolism; D011817:Rabbits; D013481:Superoxides / Q000097:blood; D010738:Type C Phospholipases / Q000494:pharmacology
D003208:Concanavalin A; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D005372:Filipin; D007770:L-Lactate Dehydrogenase; D010738:Type C Phospholipases; D007931:Leucyl Aminopeptidase; D004072:Digitonin