A previously unidentified leukotriene produced by human polymorphonuclear leukocytes.
A previously unidentified leukotriene was isolated from incubations of human polymorphonuclear leukocytes with ionophore A23187. The compound eluted between LTB4 and 20-carboxy LTB4 on SP-HPLC and between the two 6-trans isomers of LTB4 on RP-HPLC. Ultraviolet spectroscopy revealed three absorption bands at 258 nm, 268 nm and 278 nm. 1802 was incorporated by the OH at both C5 and C12. Oxidative ozonolysis indicated the presence of 5S, 12S configuration. The structure of the newly identified leukotriene is 5S, 12S-dihydroxy-icosatetraenoic acid. Stereochemistry of the double bonds and biologic activity were not investigated.
D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007975:Leukotriene B4 / Q000097:blood*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D007975:Leukotriene B4; D000001:Calcimycin