Unusual domains of human alphoid satellite DNA with contiguous non-satellite sequences: sequence analysis of a junction region.
The sequence organization of cloned segments of Human DNA carrying unusual domains of alphoid satellite was studied by restriction mapping, electron microscopy and base sequence analysis. In some cases restriction mapping revealed the absence of the typical 340 bp EcoR 1 dimer, although blot hybridizations showed the extensive presence of alphoid satellite. A variant monomeric construction was demonstrated by DNA sequencing. Furthermore, inverted repeats within these domains were detected by electron microscopy. In one case these were shown to be the result of interruptions in the satellite sequence by members of a family of repetitive, conserved elements.
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics*; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004276:DNA, Satellite; D004262:DNA Restriction Enzymes