Aluminum ions stimulate DNA synthesis in quiescent cultures of Swiss 3T3 and 3T6 cells.
Micromolar concentrations of AI3+ are shown to be strongly mitogenic for quiescent cultures of Swiss 3T3 and 3T6 cells. AI3+ caused a striking shift in the dose-response curve for the effect of fetal bovine serum on 3H-thymidine incorporation. In the absence of serum the mitogenic effect of aluminum was greatly potentiated by insulin or cholera toxin, but not epidermal growth factor or 12-0-tetradecanoyl-phorbol-13-acetate. The stimulation of DNA synthesis was maximal by 15-20 microM AI3+ X AI3+ at 100 microM had no inhibitory effect on DNA synthesis. AI3+ had no significant effect on cellular cyclic adenosine monophosphate in the presence or absence of insulin or an inhibitor of cyclic nucleotide phosphodiesterases.
D000535:Aluminum / Q000494:pharmacology*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides; D000242:Cyclic AMP / Q000032:analysis; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005347:Fibroblasts / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D051379:Mice; D008815:Mice, Inbred Strains
D017607:Aluminum Compounds; D002712:Chlorides; D007328:Insulin; D015636:Magnesium Chloride; D000077410:Aluminum Chloride; D004247:DNA; D000535:Aluminum; D000242:Cyclic AMP; D008274:Magnesium