Purine metabolizing enzymes in lymphocytes from patients with solid tumors.
The activities of five clinically important enzymes of purine metabolism have been determined in lymphocytes from 62 patients with various types of solid tumors. The activity of purine nucleoside phosphorylase was increased in all patient groups studied, i.e. small cell bronchogenic carcinoma (n = 30), carcinoma of the breast (n = 17) and other tumors (n = 15), compared to cells form normal donors. Activities of adenosine deaminase, adenine phosphoribosyltransferase (APRT), hypoxanthine (guanine) phosphoribosyltransferase (HGPRT), and 5'-nucleotidase (5'-NUC) vary little from control values, except for lower levels of APRT in lymphocytes from patients with carcinoma of the breast. In patients with small cell bronchogenic carcinoma, enzyme levels were also determined in granulocytes, where increased APRT activity was found. Following cytostatic treatment of these patients, significant decreases were seen in lymphocytic HGPRT and 5'-NUC activities.
D015720:5'-Nucleotidase; D000228:Adenine Phosphoribosyltransferase / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000201:enzymology; D002283:Carcinoma, Bronchogenic / Q000201:enzymology; D018288:Carcinoma, Small Cell / Q000201:enzymology; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000097:blood; D008175:Lung Neoplasms / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000201:enzymology*; D009708:Nucleotidases / Q000097:blood; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood
D000970:Antineoplastic Agents; D011683:Purine-Nucleoside Phosphorylase; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase