Activation of the respiratory burst of guinea pig neutrophils by dicyclohexylcarbodiimide.
DCCD activates the respiratory burst in guinea pig peritoneal neutrophils. The onset of the superoxide producing activity is preceeded by a lag, inversely proportional to the dose of the stimulant and to the temperature. Initial rates of superoxide formation exhibit different dependencies on the concentrations of DCCD and on temperature. Activation of NAD(P)H oxidase is inhibited by preincubation of neutrophils with 2-deoxyglucose and does not require the presence of extra cellular Ca2+.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002234:Carbodiimides / Q000494:pharmacology*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006168:Guinea Pigs; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D010101:Oxygen Consumption / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism
D002234:Carbodiimides; D009097:Multienzyme Complexes; D013481:Superoxides; D004024:Dicyclohexylcarbodiimide; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases