title: Nucleotide sequence of the promoter region of the melibiose operon of Escherichia coli.
abstract: The nucleotide sequence of the promoter region of the melibiose operon of E. coli was determined. Consensus sequences for the -35 region, the Pribnow box and the binding site for cyclic AMP receptor protein were found in this region. The possible secondary structure of this DNA region was very similar to that of the promoter region of the lactose operon. A possible initiation ATG preceded by a Shine-Dalgarno sequence with proper spacing was present just downstream of the promoter region. The possible sequence of 52 amino acid residues in the NH2 terminus of the alpha-galactosidase were determined.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D007763:Lac Operon; D026901:Membrane Transport Proteins / Q000235:genetics*; D009876:Operon; D011953:Receptors, Cyclic AMP / Q000302:isolation & purification; D027981:Symporters; D000519:alpha-Galactosidase / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D011953:Receptors, Cyclic AMP; D027981:Symporters; C021548:melibiose permease; D005696:Galactosidases; D000519:alpha-Galactosidase