Effect of epidermal growth factor on dipeptidyl-aminopeptidase and collagenase-like peptidase activities in cloned osteoblastic cells.
The effect of epidermal growth factor (EGF) on collagen degradation in clonal osteoblastic MC3T3-E1 cells was investigated by measuring the activities of dipeptidyl-aminopeptidase (DAP) and collagenase-like peptidase (CL-peptidase). EGF at concentrations of 2 to 50 ng/ml markedly increased DAP and CL-peptidase activities in the cells. The same concentrations of this factor significantly decreased the cellular hydroxyproline content. Since DAP and CL-peptidase are thought to be enzymes involved in collagen degradation, these results suggest that a physiological concentration of EGF stimulates collagen catabolism in osteoblasts.
D000818:Animals; D002999:Clone Cells; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D003012:Microbial Collagenase / Q000378:metabolism*; D010006:Osteoblasts / Q000201:enzymology*
D004815:Epidermal Growth Factor; D010450:Endopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D003012:Microbial Collagenase