title: [NADPase and NADase in the peritoneal macrophages of mice].
abstract: Murine peritoneal macrophages are able to hydrolyse NAD+ and NADP+. The NADPase activity exceeds that of NADase by 22-24%. The pH optima for both the enzymes are, respectively, 6.0 and 7.0. NAD hydrolysis is considerably activated by Mg2+, whereas NADP hydrolysis remains not affected. NAD+ does not change NADPase activity, while NADase activity is inhibited by NADP by 25-30%. A diazonium salt of sulfanilic acid, known to be an inhibitor of cell plasma membranes, does not affect NADP+ hydrolysis and causes a 20-30% retardation of NAD+ hydrolysis. The data obtained suggest that murine peritoneal macrophages contain two hydrolytic enzymes: NADase and NADPase.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009243:NAD; D009249:NADP; D006867:Hydrolases; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase
vernacular_title: NADFaza i NADaza peritoneal'nykh makrofagov mysheĭ.