title: Two distinct classes of prechondrogenic cell types in the embryonic limb bud.
abstract: Differences are demonstrated in the chondrogenic potential of cells derived from the distal and proximal halves of chick wing buds from as early as stage 23, prior to the appearance of overt cartilage differentiation. In high cell density cultures, cells obtained from the distal portions of stage 23 or 24 limb buds are spontaneously chondrogenic in micromass cultures. Cells obtained from the proximal portions, however, become blocked in their differentiation as protodifferentiated cartilage cels, since these cells in micromass cultures make detectable type II collagen, but fail to synthesize significant levels of cartilage proteoglycan or to accumulate an extracellular matrix that will stain for sulfated glycosaminoglycans. Such cultures of proximal limb bud cells can be stimulated to form alcian blue staining nodules by the addition of 1 mM dbcAMP or 50 micrograms/ml ascorbate, or by mixing proximal cells with small numbers of distal cells (1 distal cell to 10 proximal cells). These results demonstrate the existence of two distinct stages among prechondrogenic mesenchyme cells. The earlier stage appears to be able to provide a chondrogenic stimulus to proximal cells.
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D002449:Cell Aggregation; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003370:Coturnix / Q000196:embryology; D006025:Glycosaminoglycans / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis; D013431:Sulfates / Q000378:metabolism; D014921:Wings, Animal / Q000196:embryology*
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D003994:Bucladesine