Altered transcription of genes coding for class I histocompatibility antigens in murine tumor cells.
Three murine tumors induced by Moloney murine leukemia virus (M-MLV) which exhibited loss of some or all H-2 class I antigens at the cell surface were analyzed at the DNA and RNA level with molecular probes specific of H-2 heavy chains and beta 2-microglobulin sequences. No observable difference could be detected at the DNA level between the tumors and the parent animals. However, a decrease in H-2 mRNA was observed, especially in phenotypically H-2 negative tumor, BM5R, where H-2 transcripts were at least 30-fold less abundant. These results show that an H-2-negative character may result from a general alteration in the transcription of H-2 genes, which could reflect some kind of regulatory process.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005815:Genetic Code; D006183:H-2 Antigens / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology*; D008223:Lymphoma / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008979:Moloney murine leukemia virus / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D013945:Thymoma / Q000235:genetics / Q000276:immunology*; D014158:Transcription, Genetic; D001613:beta 2-Microglobulin / Q000235:genetics
D006183:H-2 Antigens; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D004262:DNA Restriction Enzymes