Omega 6-oxygenation of 6, 9, 12-octadecatrienoic acid in human platelets.
[1-14C]6, 9, 12-Octadecatrienoic acid was incubated with suspensions of human platelets. Three monohydroxy acids were isolated, i.e. 10LS-hydroxy-6, 8-pentadecadienoic acid, 10LS-hydroxy-6, 8, 12-octadecatrienoic acid, and 13LS-hydroxy-6, 9, 11-octadecatrienoic acid. Aspirin (0.5 mM) and indomethacin (10 microM) completely inhibited formation of the first mentioned compound whereas 5, 8, 11, 14-eicosatetraynoic acid (34 microM) inhibited formation of all three compounds. Isolation of 13LS-hydroxy-6, 9, 11-octadecatrienoic acid demonstrates that human platelets possess a lipoxygenase activity catalyzing omega 6-oxygenation of suitable poly-unsaturated fatty acids.
D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008042:Linolenic Acids / Q000097:blood*; D008084:Lipoxygenase / Q000097:blood; D017965:gamma-Linolenic Acid
D008042:Linolenic Acids; D017965:gamma-Linolenic Acid; D008084:Lipoxygenase; D001241:Aspirin; D007213:Indomethacin