Suppression of a VP1 mutant of simian virus 40 by missense mutations in serine codons of the viral agnogene.
We isolated second-site revertants of a partially defective VP1 mutant of simian virus 40. The suppressing mutation in each of these pseudorevertants was mapped to the viral agnogene. Of six independently isolated pseudorevertants, all had a missense mutation in a serine codon, near the beginning of the agnogene, that would cause replacement of serine at position 7, 11, or 17 in the agnoprotein by a hydrophobic amino acid. Our results suggest that the agnoprotein interacts in a specific way with VP1 during the late stages of viral development.
D003062:Codon; D005814:Genes, Viral; D009154:Mutation; D012694:Serine; D013539:Simian virus 40 / Q000235:genetics* / Q000254:growth & development; D013489:Suppression, Genetic; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology; D054334:Viral Regulatory and Accessory Proteins; D015678:Viral Structural Proteins; D000086082:Viroporin Proteins
D003062:Codon; D012694:Serine; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D015678:Viral Structural Proteins; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins