title: [Albumin receptors on isolated human hepatocytes. Studies by phase contrast and scanning electron microscopy].
abstract: In the present work a minibead method has been employed to study human hepatocyte receptors for albumin-coupled latex particles. The cell-latex reaction was observed in both phase contrast and scanning electron microscopy. It has been demonstrated that human hepatocytes exhibit bindings sites for different forms of glutaraldehyde-treated albumin, and the ligand has proved to be species-nonspecific. The albumin binding activity is temperature-dependent and can be only partially blocked by preincubation with free, glutaraldehyde-treated protein, unless hepatocytes are pretreated with vinblastine sulfate. The possible biological role of these receptors as regards the infection due to the hepatitis B virus (that shares common, but not identical binding sites) is also discussed.
mesh_terms: D006801:Humans; D008099:Liver / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D008863:Microspheres; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis*; D012709:Serum Albumin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D014747:Vinblastine
vernacular_title: Recettori albuminici su epatociti umani isolati. Studio in microscopia a contrasto di fase e in microscopia elettronica a scansione.