Evidence for the presence of a hybrid of phosphoglyceromutase/bisphosphoglyceromutase in the red cells: partial characterization of the hybrid.
Purified phosphoglyceromutase was hybridized in vitro with pure biphosphoglyceromutase . The hybrid showed an electrophoretic mobility identical to that of the intermediate band of red cell phosphoglyceromutase activity in the hemolysate patterns. Electrophoretic tests showed that the partially purified hybrid displayed both bisphosphoglyceromutase and phosphoglyceromutase activities and that the sample contained also a small portion of non-hybrid bisphosphoglyceromutase . By constrast to a non-hybrid mixture of the two purified mutases the hybrid exhibited heat instability of bisphosphoglyceromutase activity and neutralization of phosphoglyceromutase activity by anti- bisphosphoglyceromutase antibody.
D001731:Bisphosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000097:blood*; D002845:Chromatography / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D005033:Ethylmaleimide / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007120:Immunochemistry; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000097:blood*; D010770:Phosphotransferases / Q000097:blood*
D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase; D001731:Bisphosphoglycerate Mutase; D005033:Ethylmaleimide