A novel chromatographic procedure for purification of bacterial plasmids.
A new, rapid procedure for purifying bacterial plasmids with high recovery is described. The sequence of operations consists essentially of treatment with alkali, ribonuclease, and proteinase K, followed by chisam extraction and gel filtration on Sephacryl S-1000, and finally a precipitation step using isopropanol at room temperature. The method gives rather good yields of plasmid DNA of high purity, and lends itself to scaling up.
D001419:Bacteria / Q000032:analysis / Q000235:genetics*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes