Studies on the possible involvement of singlet oxygen and superoxide anion radicals in the cyclo-oxygenase reaction.
The influence of several naphthalene and furan derivatives which are potent singlet oxygen (1O2) acceptors on two prostaglandin synthetase preparations derived from bovine seminal vesicles or rat kidney medulla was studied. Also the effects on the formation of superoxide anion radicals (O2-.) were measured to give further evidence for or against the possible involvement of 1O2 or O2-. in the cyclo-oxygenase reaction. The data we have attained make an essential role of these activated oxygen species as initiators of the cyclo-oxygenase reaction unlikely and thereby support the hypothesis that cyclo-oxygenation during prostaglandin biosynthesis is a lipoxygenase type of reaction.
D000818:Animals; D002392:Catechin / Q000494:pharmacology; D002417:Cattle; D016861:Cyclooxygenase Inhibitors; D005609:Free Radicals; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000378:metabolism; D008297:Male; D009281:Naphthalenes / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats; D012669:Seminal Vesicles / Q000378:metabolism; D026082:Singlet Oxygen; D013481:Superoxides / Q000378:metabolism*
D016861:Cyclooxygenase Inhibitors; D005609:Free Radicals; D009281:Naphthalenes; D013481:Superoxides; D026082:Singlet Oxygen; D002392:Catechin; D011451:Prostaglandin-Endoperoxide Synthases; D010100:Oxygen