Colorimetric assay for aminoacyl-tRNA synthetases.
A simple, rapid, and inexpensive procedure for the determination of aminoacyl-tRNA synthetase activity is described. The assay is based on a green color formed between PPi and ammonium molybdate in the presence of mercaptoethanol. The sensitivity is in the nanomole range and is comparable with the conventional [32P]PPi-ATP exchange assay. Amino acid, ATP, magnesium ion, and most common reagents do not interfere with the color yield.
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D003124:Colorimetry; D011756:Diphosphates / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D010920:Placenta / Q000201:enzymology
D011756:Diphosphates; D000604:Amino Acyl-tRNA Synthetases; C019975:cysteinyl-tRNA synthetase