Solubilization, gel filtration and sedimentation behaviour of prolactin receptors from human ovarian tissue.
Receptors for 125I-labelled human prolactin have been identified in the crude membrane fraction isolated from human ovarian tissue. The non-ionic detergent Triton X-100, has been used to solubilize the membrane fraction. The presence of the receptor in the detergent extract was demonstrated by gel filtration and sucrose density gradient centrifugation. The binding was time-temperature dependent, being maximal at 23 degrees C after 15 h of incubation. Large amounts of other peptide hormones did not inhibit the binding of 125I-labelled human prolactin. The binding Scatchard analysis demonstrated that the affinity of the soluble receptor (Ka 1.13 +/- 0.15 X 10(10) M-1) for the labelled hormone was slightly greater than that of the crude membrane fraction (Ka 0.91 +/- 0.12 X 10(10) M-1). The binding capacity of the solubilized receptor was also significantly greater than that seen in the particulate before solubilization. The apparent Stokes radius of the solubilized receptor was estimated to be 57 A and that the hormone-receptor complex 60 A. The sedimentation coefficient of the solubilized receptor was 7.0 +/- 0.1 s, whereas that of the hormone-receptor complex was 7.5 +/- 0.2 s.
D000328:Adult; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007700:Kinetics; D010053:Ovary / Q000032:analysis* / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D011981:Receptors, Prolactin; D012995:Solubility; D013347:Subcellular Fractions / Q000378:metabolism
D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011388:Prolactin