[Differences in the Ca2+- and calmodulin-sensitivity of cyclic nucleotide phosphodiesterase [PDE] in microtubule preparations from brain and Ehrlich-ascites-tumor cells [EAT]].
In MTP preparations from brain and EAT (isolation by the method of Doenges et al. 1977) assembled in vitro by two cycles of polymerization and depolymerization, differences appeared in the activation of the cAMP-phosphodiesterase by Ca2+ and Calmodulin. cAMP-PDE-activity in the MTP from brain is not influenced by Ca2+ or Calmodulin. However the PDE-activity in the EAT-MTP is decreased by Ca2+ (!) and is increased by Calmodulin. cGMP-PDE-activity is present in brain-MTP, and in contrast to this it is absent in MTP from EAT.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D008870:Microtubules / Q000187:drug effects / Q000201:enzymology*; D013552:Swine
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002118:Calcium
Unterschiede in der Ca2+- und Calmodulin-Empfindlichkeit der zyklischen Phosphodiesterase (PDE) in Mikrotubulipräparationen aus Hirn und Ehrlich-Aszites-Tumorzellen (EAT).