Chromatin structure and transcriptional activity of an X-linked heat shock gene in drosophila pseudoobscura.
Nuclease digestion of isolated nuclei was used to test whether differential chromatin structure exists for a dosage-compensated heat shock gene in Drosophila pseudoobscura. No differences were observed in nuclease sensitivity at this locus in males and females, either under heat shock or non-heat shock conditions, using micrococcal nuclease or DNase I. Although the higher level of nuclease sensitivity characterized by the induced state was removed when nuclei were prepared in high salt (0.45 M sodium chloride), this procedure did not reveal covert differences in X-linked chromatin structure between males and females. However, a clear difference was observed in the nuclease sensitivity at low level (uninduced) and high level (heat-induced) expression of the X-linked heat shock gene, suggesting that the same gene transcribed at two steady state rates can have different chromatin structures.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D004706:Endodeoxyribonucleases; D005260:Female; D005796:Genes; D006360:Heat-Shock Proteins / Q000235:genetics*; D007814:Larva / Q000378:metabolism; D008836:Micrococcal Nuclease; D014158:Transcription, Genetic; D014960:X Chromosome / Q000502:physiology*
D002843:Chromatin; D006360:Heat-Shock Proteins; D004706:Endodeoxyribonucleases; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease