[CAMP mediated cell growth regulation. I. Characterization of protein kinases in CHO cells].
Protein kinases in wild-type CHO cells have been characterized. Cells cultured on MEM were collected, homogenized and the extract assayed for protein kinase activity. DEAE cellulose chromatography of 30.000xg extract yields 2 peaks of protein kinases activity, PKI and PKII. The two peaks when analyzed for the binding of 8-N3-(32P)cAMP show two subunits RI and RII and a RI not associated with the enzymatic activity, named RF. This characterization allows us to discuss the meaning of protein kinases in the modulation of the growth regulating effects of cAMP.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000502:physiology*; D005260:Female; D010053:Ovary; D011494:Protein Kinases / Q000502:physiology*
D000242:Cyclic AMP; D011494:Protein Kinases
Regolazione della crescita cellulare da parte del cAMP. I. Caratterizzazione della proteinkinasi in cellule CHO.