Effects of the stilbene derivatives SITS and DIDS on intestinal ATPase activities.
4-Acetamido-4'-isothiocyano-2,2'-disulfonic stilbene (SITS) and 4,4'-diisothiocyano-2,2'-disulfonic stilbene (DIDS) effects have been tested both in the basolateral membranes (BLMs) of jejunum enterocytes and in the same intestinal tract, everted and incubated "in vitro". Total and (Na,K)-ATPase activities of BLMs are inhibited in a similar way by the two disulfonic stilbenes as well as the fluid transintestinal transport in the everted intestine; on the contrary cell Na and K are unaffected. SITS and DIDS inhibition of (Na,K)-ATPase seems to take place at the cytoplasmic side of the BLM.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000201:enzymology*; D008566:Membranes / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*
D013267:Stilbenes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D010042:Ouabain; D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D011190:Potassium Cyanide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid