[Peculiarities of the functioning of the mitochondrial genome in experimental alloxan diabetes].
Biosynthesis of RNA was studied in mitochondria of heart muscle, liver, kidney and spleen tissues of normal rats and of the animals with experimental alloxane diabetes. In the diabetes biosynthesis of RNA was distinctly decreased in mitochondria of all the tissues studied, especially in the mitochondria of heart muscle. As shown by means of molecular hybridization of RNA-DNA, using total DNA from mitochondria and DNA of recombinant plasmides containing restrictional fragments of rat mitochondrial DNA, the decrease in mitochondrial RNA biosynthesis under conditions of the diabetes was not due to selective inhibition of individual genes transcription but it was determined by a decrease in expression of all the mitochondrial genes simultaneously.
D000818:Animals; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D051381:Rats; D013154:Spleen / Q000378:metabolism*; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes
Osobennosti funktsionirovaniia mitokhondrial'nogo genoma pri éksperimental'nom alloksanovom sakharnom diabete.