Effect of antibody-induced modulation of measles (SSPE) virus membrane proteins on beta-adrenergic receptor-mediated adenylate cyclase activity.
The effect of measles virus antiserum on the expression of viral glycoproteins on the membranes of measles (SSPE) persistently infected C6 rat glioma cells was studied. There was a gradual loss of virus membrane antigen from these cells until no antigen was detectable 18 days after initiation of antiserum treatment. At this stage approximately 25% of cells still displayed intracellular virus antigen which was also lost after further cell passages. There was an accompanying recovery of the previously reported disrupted catecholamine-dependent beta-adrenergic receptor-stimulated cAMP synthesis in antiserum-treated cells which coincided with the loss of viral antigen from the membrane. This was determined to be due to a recovery of fluoride-stimulated activity of the cAMP synthesising adenylate cyclase enzyme to normal values. Thus we demonstrate that the impairment of this important cell function was due to insertion of viral antigen in the cell membrane rather than its accumulation in the cytoplasm.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D000956:Antigens, Viral; D001254:Astrocytoma; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D000242:Cyclic AMP / Q000096:biosynthesis; D007106:Immune Sera / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008459:Measles virus / Q000276:immunology* / Q000502:physiology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D013190:SSPE Virus / Q000276:immunology* / Q000502:physiology
D000956:Antigens, Viral; D007106:Immune Sera; D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D007545:Isoproterenol