Use of a cloned DNA fragment to analyze the fate of donor DNA in transformation of Streptococcus pneumoniae.
The integration of donor label into the recipient fragment is followed during transformation of Streptococcus pneumoniae. The method used involves gel analysis of restriction endonuclease-treated recipient DNA after recombination with a radioactively labeled homologous cloned fragment.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004247:DNA; D004262:DNA Restriction Enzymes