Differential extraction of tumor-specific transplantation antigen and embryonic antigen from simian virus 40- and adenovirus 7-induced sarcoma cells of hamsters with 1-butanol and 3 M potassium chloride.
Simian virus 40 (SV40)-induced sarcomas and adenovirus 7-induced sarcomas (Adv-7) exhibit both specific tumor-specific transplantation antigens (TSTA) and cross-protective embryonic antigens at the cell surface in the LAK:LVG(SYR) strain of Syrian golden hamsters. Specific SV40 TSTA could be released from the surfaces of living hamster sarcoma cells in a 2.5% crude 1-butanol extract (CBE) and served as immunogen to protect syngeneic recipients against subsequent homologous but not heterologous tumor cell challenge. The CBE-extracted SV40-induced TSTA (tumor-specific) was observed to be free of detectable, cross-protective embryonic antigens (EA) by tumor transplantation assays. The induction of cytotoxic lymphocyte-mediated immunity with the CBE-released TSTA was dependent on the administration of a single sensitizing injection of 12-20 micrograms antigen protein. Higher concentrations (50-1,000 micrograms) of the CBE tumor cell extract, given in a single injection, enhanced tumor growth as did two injections of 12.5 micrograms CBE-extracted SV40-induced TSTA at 1-week intervals. A cross-protective antigen(s), not detected in the CBE tumor extracts, was retained in the intact, 1-butanol-extracted SV40 and Adv-7-induced tumor cell lines after completion of the CBE extraction procedure and in similarly extracted 10-day hamster fetal cells. Some alterations in the normal immunogenicity of EA extracted with CBE followed by KCI from SV40-induced sarcoma cells could be detected in the transplantation assays and lymphocyte transformation assays, whereas EA extracted from CBE-KCI-treated Adv-7 cells or 10-day hamster fetal cells retained normal immunogenicity in vivo and in vitro. These procedures provide a means for successful separation of immunogenic SV40- and Adv-7-induced TSTA from detectable, biologically active, cross-protective EA from the surfaces of these sarcoma cells.
D020001:1-Butanol; D000256:Adenoviridae; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D000440:Butanols; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D006224:Cricetinae; D005333:Fetus / Q000276:immunology; D006649:Histocompatibility Antigens / Q000008:administration & dosage / Q000302:isolation & purification*; D007114:Immunization; D008297:Male; D008647:Mesocricetus; D011189:Potassium Chloride; D012509:Sarcoma / Q000276:immunology* / Q000473:pathology; D013539:Simian virus 40; D014412:Tumor Virus Infections
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000440:Butanols; D006649:Histocompatibility Antigens; C029237:tumor-associated transplantation antigen; D011189:Potassium Chloride; D020001:1-Butanol