Expression of functional acetylcholine receptor from cloned cDNAs.
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the alpha-subunit is indispensable for alpha-bungarotoxin binding activity.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D046911:Macromolecular Substances; D009865:Oocytes / Q000502:physiology; D011950:Receptors, Cholinergic / Q000235:genetics*; D013329:Structure-Activity Relationship; D014101:Torpedo; D014982:Xenopus laevis
D046911:Macromolecular Substances; D011950:Receptors, Cholinergic; D004247:DNA