Bone-marrow derived macrophages as targets for the replication of mouse hepatitis virus type 3.
Bone-marrow (BM) derived macrophages are sensitive target cells for replication of mouse hepatitis virus type 3 (MHV3). These cells can be grown in large numbers and the percentage of defined macrophages increased until day 10 when 100% of the cells represented macrophages. MHV3 replicated within these cells to high titers and caused the formation of multi-nucleated giant cells. This effect was seen with very low virus inocula in BM macrophages of C57BL/6 mice that are highly susceptible to in vivo infection with MHV3 whereas macrophages from resistant A/J mice did not show a cytopathic effect at these virus doses. 1000-fold higher virus doses, however, caused the cytopathic effect in macrophages of both C57BL/6 and A/J mice.
D000818:Animals; D001202:Ascitic Fluid / Q000382:microbiology; D001853:Bone Marrow / Q000382:microbiology*; D001854:Bone Marrow Cells; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D008264:Macrophages / Q000382:microbiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D006517:Murine hepatitis virus / Q000502:physiology*; D014779:Virus Replication