Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection.
Specific antibodies to cell membrane antigens found on human T-cell leukemia virus (HTLV)-infected cells have been detected in Japanese patients with adult T-cell leukemia/lymphoma and in asymptomatic carriers, using a live cell-membrane immunofluorescence assay. Reactivity of the positive antisera was analyzed using radioimmunoprecipitation and NaDodSO4/PAGE with the HTLV-infected tumor cell line Hut 102 (clone B2). The major cell-associated antigens identified include two glycoproteins of approximately equal to 61 and 45 kDa, which appear to be the most immunogenic species in exposed people, a nonglycosylated species of 42 kDa, and four additional species that contain gag gene-encoded antigens with sizes ranging from 19 to 55 kDa. The two glycoproteins ( gp61 and gp45 ) are encoded, at least in part, by the env gene of HTLV as evidenced by amino acid sequence analysis.
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002472:Cell Transformation, Viral; D017977:Deltaretrovirus / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D008970:Molecular Weight; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins