[Molecular organization of glutamate-sensitive, chemoexcitatory membranes of nerve cells. Physico-chemical characteristics of the glutamate-binding synaptic membrane proteins of the rat cerebral cortex].
Some physico-chemical properties of glutamate-binding proteins solubilized from rat cerebral cortex synaptic membranes and purified by affinity chromatography were studied. Purified proteins were shown to be homogenous during SDS polyacrylamide gel electrophoresis (Mr 14000). The Scatchard plots for L-[3H]glutamate binding to the purified membrane proteins revealed the presence of one type of binding sites with Kd 800-1000 nM and Bmax 180-200 pmol/mg of protein. Ultracentrifugation of the glutamate-binding membrane protein in sucrose linear gradient demonstrated that the position of the protein peak depends on protein concentration, i.e. after dilution of the sample the protein peak is shifted from 28 000-30 000 to 12 000-15 000. The values of sedimentation coefficients decrease correspondingly to 2.1S. Presumably, these processes are due to dissociation of receptor macromolecules. The glutamate receptor is a glycoprotein-lipid complex made up of several low molecular weight subunits.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism; D017470:Receptors, Glutamate; D013570:Synaptic Membranes / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate
Molekuliarnaia organizatsiia glutamatchuvstvitel'nykh khemovozbudimykh membran nervnykh kletok. Fiziko-khimicheskaia kharakteristika glutamatsviazyvaiushchikh belkov sinapticheskikh membran kory golovnogo mozga krys.