Regulation of gelatinase in human skin organ cultures by glucocorticoids.
Hydrocortisone and dexamethasone prevent the appearance of gelatinase in serum-free explant cultures of normal human skin. Hydrocortisone inhibits maximally at 10(-6) M and dexamethasone at 10(-8) M in culture medium. Glucocorticoids at these concentrations do not cause a generalized decrease in protein synthesis; thus the effect on gelatinase shows specificity. The reduction in gelatinase activity caused by dexamethasone can be overcome in the presence of dexamethasone 21-mesylate, a glucocorticoid antagonist that binds irreversibly to the cytoplasmic steroid receptor. These data suggest that the enzymes of collagen degradation, collagenase and gelatinase, may be coregulated.
D003864:Depression, Chemical; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018093:Gelatinases; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D003012:Microbial Collagenase / Q000378:metabolism; D009924:Organ Culture Techniques; D010434:Pepsin A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012867:Skin / Q000201:enzymology*
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D010434:Pepsin A; D018093:Gelatinases; D003012:Microbial Collagenase; D006854:Hydrocortisone