title: Single K+-channel current measurements from brain synaptosomes in lipid bilayers.
abstract: Ion channels from a rat brain preparation enriched in presynaptic nerve terminals (synaptosomes) were incorporated into planar lipid bilayers. Experiments examined macroscopic (channel-ensemble) currents as well as single-channel currents. Four single-channel conductances (ranging from 10 to 40 pS) were usually observed, each with distinct kinetic properties. All the observed channels selected for K+ over Cl-. These K+ channels may contribute to the resting K+ conductance of brain nerve terminals. Furthermore, this report demonstrates that the properties of ion channels from mammalian brain can be studied in planar lipid bilayers and suggests that this system can be readily extended to many additional investigations on the electrical properties of brain membranes.
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D008051:Lipid Bilayers; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D013570:Synaptic Membranes / Q000502:physiology; D013574:Synaptosomes / Q000502:physiology*
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D011188:Potassium