Prevention of rotavirus-induced diarrhea in neonatal mice born to dams immunized with empty capsids of simian rotavirus SA-11.
Female CF-1 mice were immunized subcutaneously with a rotavirus vaccine consisting of noninfectious, purified empty capsids from simian rotavirus SA-11 and were given a booster dose at 14-17 days of gestation. The vaccinated animals developed high titers of rotavirus-specific IgG in colostrum and milk. Virus-specific IgA titers were significantly (150-fold) lower, and virus-specific IgM was not detected. These results contrast with those obtained in natural and experimental infections of the gastrointestinal tract with epizootic-diarrhea infant-mouse ( EDIM ) virus; in such cases, intestinal and lacteal virus-specific antibody is primarily of the IgA isotype. Suckling mice born to vaccinated dams were challenged with infectious EDIM virus and monitored for the development of diarrheal disease; these neonates acquired maternal antibody and did not develop diarrhea.
D000818:Animals; D000831:Animals, Newborn; D002213:Capsid / Q000276:immunology; D003967:Diarrhea / Q000517:prevention & control*; D005260:Female; D007112:Immunity, Maternally-Acquired; D007117:Immunization, Secondary; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007422:Intestines / Q000276:immunology; D008297:Male; D051379:Mice; D008892:Milk / Q000276:immunology; D011247:Pregnancy; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000517:prevention & control*; D014611:Vaccination; D014765:Viral Vaccines
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014765:Viral Vaccines