[Incorporation of Na,K-ATPase into human erythrocyte membranes using liposomes].
A procedure for incorporation of isolated cattle brain Na,K-ATPase into erythrocyte membranes by proteoliposomes has been elaborated. The Na,K-ATPase activity of proteoliposome-treated human erythrocytes containing incorporated Na,K-ATPase does not exceed that of control erythrocytes. In the erythrocyte membrane the incorporated enzyme exists in a functionally active state and retains the vector properties of the Na+-pump. Exogenous ATP stimulates 22Na influx and 86Rb efflux in and from the erythrocytes.
D000818:Animals; D001693:Biological Transport, Active; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007473:Ion Channels; D008081:Liposomes / Q000008:administration & dosage*; D011868:Radioisotopes; D012413:Rubidium; D012964:Sodium / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D007473:Ion Channels; D008081:Liposomes; D011868:Radioisotopes; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium
Vstraivanie Na,K-ATPazy v membrany éritrotsitov cheloveka s pomoshch'iu liposom.