title: Human anterior uvea synthesizes lipoxygenase products from arachidonic acid.
abstract: Arachidonic acid is metabolized into biologically active prostaglandins, thromboxanes, and lipoxygenase products, leukotrienes. In the present study, the ability of human anterior uvea to synthesize lipoxygenase products from 14C-radiolabelled arachidonic acid is assessed. Following cyclooxygenase inhibition by indomethacin, human anterior uvea, similar to rabbit conjunctiva and anterior uvea, synthesizes chemotactic products 12-hydroxyeicosatetraenoic acid (HETE) and 5,12-DiHETE, indicating the presence of both 5- and 12-lipoxygenase enzyme activities.
mesh_terms: D001095:Arachidonic Acids / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007975:Leukotriene B4 / Q000096:biosynthesis*; D013189:SRS-A / Q000096:biosynthesis; D014602:Uvea / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D013189:SRS-A; D007975:Leukotriene B4; D007213:Indomethacin