Histone H1-stimulated phosphorylase phosphatase from rabbit skeletal muscle.
A major rabbit skeletal muscle phosphorylase phosphatase activity which is markedly stimulated by histone H1 has been resolved from inhibitor-sensitive phosphorylase phosphatase (type-1 phosphatase), glycogen synthase kinase 3-activated phosphatase, phosphatase heat-stable inhibitor proteins, and alkaline phosphatase activity by various purification techniques. Evidence is presented that this phosphatase is a high-molecular weight form of a type-2 phosphatase. Our data suggest that this phosphatase may be regulated by histone H1, protamine or analogous polycationic compounds.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D006657:Histones / Q000494:pharmacology*; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010765:Phosphorylase Phosphatase / Q000378:metabolism*; D011817:Rabbits
D006657:Histones; D010749:Phosphoprotein Phosphatases; D010765:Phosphorylase Phosphatase