Involvement of lysine residues in the binding of ovine chorionic somatomammotropin to lactogenic and somatotropic receptors.
The biological activities of several ovine chorionic somatomammotropin (oCS) derivatives obtained by chemical modification of the lysine residues were studied by radioreceptor assays using rabbit mammary homogenates (lactogenic activity, L.A.) and liver homogenates (somatotropic activity, S.A.). Even if the control treatment with BH-4 markedly decreased the L.A., it was clear that methylation mainly affected the S.A. and that ethylation reduced both activities. Guanidination inactivated almost completely both activities and acetimidination at a very low degree (3 of 14 lysines) led to less than 50% of both activities. These results show the involvement of lysine residues in the interaction of oCS with lactogenic and somatotropic receptors.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D007096:Imidoesters / Q000494:pharmacology; D007774:Lactation; D008099:Liver / Q000378:metabolism*; D008239:Lysine; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008782:Methylurea Compounds / Q000494:pharmacology; D010928:Placental Lactogen / Q000302:isolation & purification / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018000:Receptors, Peptide; D011986:Receptors, Somatotropin; D012756:Sheep
D007096:Imidoesters; D008782:Methylurea Compounds; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D011986:Receptors, Somatotropin; C028202:placental lactogen receptor; C014539:ethyl acetimidate; C002249:O-methylisourea; D013006:Growth Hormone; D010928:Placental Lactogen; D008239:Lysine