Anti-interferon antibody increases rhinovirus isolation rates from nasal wash specimens containing interferon-alpha 2.
Isolation of rhinoviruses from nasal secretions may be delayed or prevented by exogenously applied interferon which is carried over into nasal wash specimens. In studies of interferon-alpha 2 treated volunteers with experimental rhinovirus infection, we determined that incorporation of antiinterferon antibody into collection broth significantly increased isolation rates (84% increase) and the proportion of monolayers positive for CPE (155% increase), as compared to plain collection broth.
D000906:Antibodies / Q000008:administration & dosage*; D002478:Cells, Cultured; D003139:Common Cold / Q000276:immunology* / Q000382:microbiology; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D007370:Interferon Type I / Q000276:immunology* / Q000494:pharmacology; D012229:Rhinovirus / Q000302:isolation & purification*
D000906:Antibodies; D007370:Interferon Type I