Localization of the simian virus 40 small t antigen in the nucleus and cytoplasm of monkey and mouse cells.
Monkey and mouse cells producing simian virus 40 small t antigen in the absence of clearly detectable intact or truncated large T antigens were subjected to indirect immunofluorescence and biochemical cell compartment analyses. Results revealed specific immunofluorescence and small t polypeptide in both the nucleus and cytoplasm of these cells.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D002999:Clone Cells; D003593:Cytoplasm / Q000032:analysis; D005455:Fluorescent Antibody Technique; D008715:Methionine / Q000378:metabolism; D051379:Mice; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics*; D013462:Sulfur Radioisotopes; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D000952:Antigens, Polyomavirus Transforming; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine