title: [Effect of cyclic nucleotides on the morphogenetic and ultrastructural characteristics of the primitive knot in the chick embryo in organotypic cultures].
abstract: The isolated Hensen's nodes of chick embryos (stage 4 after Hamburger and Hamilton) were cultivated in organotypic cultures after the treatment with 0.5 mM solutions of cAMP and cGMP. Both the control and treated explants were characterized by a wide variability of differentiation of various tissue rudiments. cGMP inhibited reliably the differentiation of notochord. At the ultrastructural level the control and cAMP-treated cultures were characterized by an increased autophagia whereas the treatment with cGMP inhibited the yolk utilization and resulted in the appearance of vast regions of partial necrosis.
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D005775:Gastrula / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques
chemical_list: D000242:Cyclic AMP; D006152:Cyclic GMP
vernacular_title: Vliianie tsiklicheskikh nukleotidov na morfogeneticheskie i ul'trastrukturnye osobennosti pervichnogo uzelka émbriona kuritsy v orgnaotipicheskikh kul'turakh.