title: Evaluation of immobilized boronates for studies of adenine and pyridine nucleotide metabolism.
abstract: Three different immobilized boronates were compared with respect to their possible utility in studies of adenine and pyridine nucleotide metabolism. These included boronate derivatives of polyacrylamide, Sepharose, and the cation-exchange resin, Bio-Rex 70. The relative binding affinities, binding capacities, and elution properties were compared. Under the conditions utilized, the Sepharose and Bio-Rex 70 derivatives selectively retained nucleotides containing two or more sets of 1,2 cis-diol groups. Since the bulk of cellular nucleic acids contain but a single set of 1,2 cis-diol groups or less, these boronate derivatives are very useful for the isolation of the acid-soluble nucleotides, NAD and diadenosine 5',5"'-tetraphosphate, and for the acid-insoluble polymer, poly(ADP-ribose). The Bio-Rex 70 derivative had a particularly useful combination of binding selectivity, capacity, and elution characteristics. Specific applications of this resin for studies of NAD and poly(ADP-ribose) metabolism are presented.
mesh_terms: D000227:Adenine Nucleotides / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001897:Boronic Acids; D002411:Cation Exchange Resins; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D005347:Fibroblasts; D051379:Mice; D008807:Mice, Inbred BALB C; D009243:NAD / Q000302:isolation & purification* / Q000378:metabolism; D011064:Poly Adenosine Diphosphate Ribose / Q000302:isolation & purification / Q000378:metabolism; D012117:Resins, Synthetic; D013539:Simian virus 40; D012995:Solubility
chemical_list: D000227:Adenine Nucleotides; D001897:Boronic Acids; D002411:Cation Exchange Resins; D012117:Resins, Synthetic; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; C026257:Bio-Rex 70