A simple spectrophotometric determination of formaldehyde and other aldehydes: application to periodate-oxidized glycol systems.
A simple, rapid, and sensitive spectrophotometric assay procedure for the determination of as low as 2 microM solutions of formaldehyde and acetaldehyde using an alkaline 4-amino-5-hydrazino-3-mercapto-1,2,4-triazole reagent is described. The method is particularly useful for determination of these aldehydes (0.5-50 nmol) when produced by the periodate oxidation of various glycols and can be applied to the assay of dilute solutions of sugars or polyols.
D000079:Acetaldehyde / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D005557:Formaldehyde / Q000032:analysis*; D006018:Glycols; D010084:Oxidation-Reduction; D010504:Periodic Acid; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds
D006018:Glycols; D013438:Sulfhydryl Compounds; D010504:Periodic Acid; C006537:4-amino-5-hydrazino-1,2,4-triazole-3-thiol; D005557:Formaldehyde; C009288:metaperiodate; D000079:Acetaldehyde