Molecular cloning and restriction mapping of a simian virus 40 deletion mutant derived from simian transformants expressing a non-karyophilic T antigen.
A replication-defective Simian virus 40 genome, with a deletion of about 120 nucleotides in the region encoding the N-terminal fourth of the large T antigen, has been isolated from the DNA of Simian cells transformed by SV40. Both the original transformants, and the murine transformants obtained by transfection with this cloned mutant DNA, produced a large T antigen displaying in immunofluorescence an exclusively cytoplasmic localization. The protein apparent molecular mass (83 kDa) was about 6% smaller than that of normal karyophilic large T. Restriction analysis showed that the deletion eliminated two close HinfI sites, at nucleotides 4459 and 4376 (map unit 0.50).
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D051379:Mice; D008970:Molecular Weight; D009154:Mutation; D009693:Nucleic Acid Hybridization; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes