A new method for reconstitution of highly fusogenic Sendai virus envelopes.
A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.
D004279:DNA, Viral / Q000302:isolation & purification; D003902:Detergents; D004912:Erythrocytes / Q000502:physiology; D006023:Glycoproteins / Q000502:physiology*; D006461:Hemolysis; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology; D017830:Octoxynol; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*; D010743:Phospholipids / Q000502:physiology; D011092:Polyethylene Glycols; D014759:Viral Envelope Proteins / Q000502:physiology*
D004279:DNA, Viral; D003902:Detergents; D006023:Glycoproteins; D008565:Membrane Proteins; D010743:Phospholipids; D014759:Viral Envelope Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol