title: Ethoxyformylation of bovine growth hormone.
abstract: Reactivity of histidines in bovine growth hormone towards ethoxyformic anhydride was investigated and localization in the molecule of two kinetically distinguishable classes was achieved, a slow class including only histidine residue 169 (k = 0.180 min-1) and a fast one composed of histidines 19 and 21 (k = 0.900 min-1). Total ethoxyformylation of bovine growth hormone brought about a complete loss of its capacity to compete with 125I-labelled hormone for rat-liver binding sites, but modification of approximately half of the fast histidine group was enough to produce an important decrease in this capacity. Circular dichroism studies indicated no significant changes in protein conformation with all three histidine residues modified. Practically full binding capacity was restored when these residues were regenerated by treatment with hydroxylamine. These results suggest that one or both of the fast reacting histidine residues are involved in bovine growth hormone binding to its specific receptors.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001835:Body Weight / Q000187:drug effects; D002417:Cattle; D004047:Diethyl Pyrocarbonate; D005561:Formates; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006639:Histidine; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011986:Receptors, Somatotropin
chemical_list: D005561:Formates; D007202:Indicators and Reagents; D011956:Receptors, Cell Surface; D011986:Receptors, Somatotropin; D006639:Histidine; D013006:Growth Hormone; D004047:Diethyl Pyrocarbonate