Interleukin 3-dependent hematopoietic progenitor cell lines.
Several biological phenotypes of growth factor-dependent cell lines have been described in recent years, including those with T lymphocyte, neutrophil granulocyte, basophil/mast cell, B lymphocyte, and multipotential stem cell properties. The growth factors for each cell lineage are a subject of intense study. Continuous mouse bone marrow cultures infected with RNA type C viruses (retroviruses) produce nonadherent hematopoietic cells over a longer duration than control cultures. Marrow cultures derived from strains with spontaneously induced ecotropic endogenous retrovirus demonstrate a greater longevity than those from strains with no replicating virus. Cultures infected with murine leukemia virus also generate a greater number, compared with controls, of cloned permanent suspension cell lines dependent for growth on a 41,000-dalton glycoprotein (interleukin 3 [IL 3]). Some are multipotential with capacity for differentiation to erythroid, neutrophil, eosinophil, and basophil/mast cell types. Other cloned IL 3-dependent cell lines are committed to a single pathway. Studies with Friend spleen focus-forming virus indicate that the first effect in the marrow culture is mediated through a subset of adherent hematopoietic stem cells. Bone marrow culture-derived IL 3-dependent cell lines provide a model with which to study the role of viral genes in the control of differentiation and self-renewal capacity of hematopoietic stem cells.
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007377:Interleukin-3; D009052:Leukemia Virus, Murine / Q000235:genetics; D007942:Leukemia, Experimental / Q000382:microbiology; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009113:Muramidase / Q000032:analysis; D011086:Polycythemia / Q000503:physiopathology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000502:physiology
D007377:Interleukin-3; D008222:Lymphokines; D009113:Muramidase