Inhibition of human acute lymphoblastic leukemia cells by immunotoxins: potentiation by chloroquine.
Immunotoxins containing pokeweed antiviral protein and monoclonal antibodies against human T cells or human transferrin receptor efficiently killed acute lymphoblastic leukemia cells. Chloroquine specifically enhanced the rate of protein synthesis inhibition by immunotoxin. Depending on its concentration, chloroquine (10 to 100 micromolar) reduced by up to 65-fold the amount of immunotoxin required to inhibit protein synthesis in the target cells 50 percent.
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology* / Q000627:therapeutic use; D003131:Combined Modality Therapy; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism* / Q000628:therapy; D009699:N-Glycosyl Hydrolases; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010940:Plant Proteins / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000276:immunology; D011990:Receptors, Transferrin; D054789:Ribosome Inactivating Proteins, Type 1; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D054789:Ribosome Inactivating Proteins, Type 1; D002738:Chloroquine; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein