Transforming potential of deletion mutants of the SV40 T antigen coding gene in Syrian hamster cells.
Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selection and cloning, the TK-positive transformants that also expressed T antigen were tested for the extent of their transformation with respect to a number of characteristics, which included saturation density, ability to grow in soft agar, resistance to butyrate and to dibutyryl-cAMP, and plating efficiency. The combined results of these various tests indicate that cells containing partially deleted SV40 T antigen-coding genes are less transformed than cells containing an intact SV40 T antigen-coding gene. However, the amounts of T antigen are lower in cells transformed by deletion mutants than in cells transformed by wild-type T antigen-coding gene. Our data indicate that both the quantity and the quality of T antigen may be important in determining the degree of transformation in Syrian hamster cells.
D000362:Agar; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis / Q000235:genetics*; D003994:Bucladesine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D006224:Cricetinae; D003470:Culture Media; D005814:Genes, Viral; D008647:Mesocricetus; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014162:Transfection
D000957:Antigens, Viral, Tumor; D002087:Butyrates; D003470:Culture Media; D020148:Butyric Acid; D003994:Bucladesine; D000362:Agar