title: Catalytic activity of neutrophil and eosinophil peroxidase. Cytochemical and biochemical study in total hereditary myeloperoxidase deficiency.
abstract: We studied myeloperoxidase and eosinophil peroxidase activity in healthy subjects and in four totally myeloperoxidase deficient subjects using cytochemical and biochemical methods. Cytochemical tests demonstrated a pH dependent, enzymatic differentiated affinity of myeloperoxidase and eosinophil peroxidase for the chromogens used. The eosinophil peroxidase/myeloperoxidase ratio varied from 1.5 to 4. Biochemical assays, using MBTH-AA or o-dianisidine methods, produced eosinophil peroxidase/myeloperoxidase ratios of 1.98 and 1.72.
mesh_terms: D004804:Eosinophils / Q000201:enzymology*; D006651:Histocytochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000172:deficiency*; D010544:Peroxidases / Q000097:blood* / Q000172:deficiency*
chemical_list: D010544:Peroxidases; D009195:Peroxidase