Characterization of Epstein-Barr virus-related thymidine kinase induced in nonproducer cells by superinfection or chemical treatment.
Chemical treatment with a combination of 12-O-tetradecanoylphorbol-13-acetate and n-butyrate or superinfection with P3HR-1 virus effectively induced a novel thymidine kinase (TK) in Raji and NC37 TK-negative mutant cells. Sera from patients with nasopharyngeal carcinoma containing a high antibody titer against Epstein-Barr virus early antigen could inactivate this induced enzyme while sera from normal donors, even those containing a high titer of antibodies to virus capsid antigen, had no effect on the enzyme. Also, the induced enzyme was relatively insensitive to thymidine triphosphate inhibition.
D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D002472:Cell Transformation, Viral; D004790:Enzyme Induction / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D009303:Nasopharyngeal Neoplasms / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D013942:Thymine Nucleotides / Q000494:pharmacology
D002087:Butyrates; D013942:Thymine Nucleotides; D020148:Butyric Acid; D013937:Thymidine Kinase; D013755:Tetradecanoylphorbol Acetate; C024157:thymidine 5'-triphosphate