[Cloning in bacterial and yeast vectors of the hepatitis B virus genome].
Hepatitis B viral genome was cloned simultaneously with bacterial plasmides pBR 325 and pBR 322. Recombinant plasmides were constructed, containing DNA of hepatitis B virus as well as capable to replication in bacterial and yeast cells. Replication of one of these plasmides was possible if it integrated with chromosomal DNA of yeast target-cells; the other plasmides had a capacity to autonomous replication in yeast cells.
D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Klonirovanie v bakterial'nykh i drozhzhevykh vektorakh genoma virusa gepatita B.