1 alpha,25-Dihydroxyvitamin D3 markedly enhances chemically-induced transformation in BALB 3T3 cells.
1 alpha,25-Dihydroxyvitamin D3, a hormonally active form of vitamin D3, enhanced methylcholanthrene-induced transformation in BALB 3T3 cells to a much greater extent than 12-O-tetradecanoylphorbol-13-acetate. This enhancement was probably mediated by a cytosol receptor for 1 alpha,25-dihydroxyvitamin D3 which has an equilibrium constant of 28.4pM and a maximum binding of 32.6 fmol/mg protein.
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D011485:Protein Binding; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D008748:Methylcholanthrene; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate