Inherited susceptibility to retrovirus-induced transformation of Gardner syndrome cells.
Skin fibroblasts from patients with Gardner syndrome (GS), those with familial polyposis coli (FPC), and spouse or unrelated controls were karyotyped and tested for various growth properties including susceptibility to transformation by viral or chemical agents. Our results indicated that based on the higher susceptibility to retrovirus-induced transformation and chromosomal aneuploidy, the GS and FPC cells could be distinguished from those of the general population with more than 70% accuracy. However, much work is in order before any biological assay can be used for clinical diagnosis of GS or FPC patients.
D000782:Aneuploidy; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts; D005736:Gardner Syndrome / Q000235:genetics* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007417:Intestinal Polyps / Q000235:genetics / Q000473:pathology; D007621:Karyotyping; D007708:Kirsten murine sarcoma virus; D008297:Male; D010375:Pedigree; D009053:Sarcoma Viruses, Murine