Replication of herpes simplex virus in two cell systems derived from rhesus monkeys.
The replication of herpes simplex virus (HSV) in two cell systems derived from rhesus monkeys (LLC-MK2 and DBS-FRhL-2) was studied. In LLC-MK2, the growth of HSV-1 was abortive or extremely limited regardless of the multiplicity of infection, while that of HSV-2 was productive only on infection at high multiplicities. DBS-FRhL-2 cells supported growth of both types of HSV, although growth was highly dependent on the age of monolayers and the infectious dose of virus inocula. Plaques were produced in DBS-FRhL-2 cell monolayers inoculated with HSV-2 but not with HSV-1, although the efficiency of their formation in the former system was much less than in a system of FL and HSV-2. On the other hand, plaques were not produced in LLC-MK2 cell monolayers by either type of HSV. The growth of adapted variants of HSV-1 was also studied. In contrast to the parental strain, these variants replicated well in LLC-MK2 even at a low multiplicity of infection and produced clear plaques in the monolayers. Furthermore, persistent infections of HSV-2 were established in DBS-FRhL-2 cell monolayers under routine culture conditions.
D000327:Adsorption; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003588:Cytopathogenic Effect, Viral; D007668:Kidney; D008168:Lung; D008251:Macaca; D008253:Macaca mulatta; D008297:Male; D018139:Simplexvirus / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors; D010948:Viral Plaque Assay; D014779:Virus Replication