Diglyceride/monoglyceride lipases pathway is not essential for arachidonate release in thrombin-activated human platelets.
Human platelets prelabeled with arachidonate exhibited a rapid and transient rise in arachidonoyl monoglyceride in addition to arachidonoyl diglyceride following thrombin stimulation. Substantial release of arachidonate and its metabolites also occurred at the early phase. Preincubation of labeled platelets with RHC 80267, a potent inhibitor of diglyceride lipase, prior to thrombin stimulation abolished the transient rise in monoglyceride but not the increase in diglyceride and the release of arachidonate and its metabolites. These results suggest that diglyceride does metabolize to monoglyceride and release arachidonate in intact platelets. However, the diglyceride/monoglyceride lipases pathway does not appear to be essential in releasing arachidonate during thrombin stimulation.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003512:Cyclohexanones / Q000494:pharmacology; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism; D006801:Humans; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008994:Monoacylglycerol Lipases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology*
D001095:Arachidonic Acids; D003512:Cyclohexanones; D004075:Diglycerides; D005989:Glycerides; D016718:Arachidonic Acid; C036535:1,6-bis(cyclohexyloximinocarbonyl)hexane; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D002265:Carboxylic Ester Hydrolases; D008994:Monoacylglycerol Lipases; D008071:Lipoprotein Lipase; D013917:Thrombin