An unusual 5' splice sequence is efficiently utilized in vivo.
The minor adult chicken alpha-globin gene (alpha D) has an intron splicing sequence at the 5' end of the second intron that begins with the dinucleotide G-C rather than the usual G-T. To understand what role this splice sequence plays in the processing and maturation of nuclear RNA to cytoplasmic RNA, we have analyzed the intron processing of the alpha D-globin transcript in both heterologous (monkey) and homologous (chicken) cells using simian virus 40 and retrovirus vectors, respectively. In both cell types, both introns of the alpha D-globin gene are efficiently and precisely removed.
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping; D004720:Endonucleases; D005822:Genetic Vectors; D005914:Globins / Q000235:genetics*; D012326:RNA Splicing; D013539:Simian virus 40 / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases
D005914:Globins; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases