A new method of evaluating complement mediated lysis of liposomes.
Immune lysis of liposomes has been utilized in immunoassays for detection of specific antibodies. Membrane immunoassays which have sensitivities comparable with those of radioimmunoassays are easy and rapid to perform. Information obtained so far has been limited by the fact that the method is based on empirical relationships. The present paper describes a simple theoretical model for analysing membrane immunoassay data. According to the model, relative dissociation constants for the antibody-antigen reaction and relative concentrations of high affinity antibodies for different antisera may be determined. Furthermore, the analysis yields the percentage of antibody bound to antigen under certain conditions. The method is also useful in the search for optimum experimental conditions in membrane immunoassay measurements.
D000818:Animals; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D002308:Cardiolipins / Q000276:immunology; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins / Q000502:physiology*; D004578:Electron Spin Resonance Spectroscopy; D006168:Guinea Pigs; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008081:Liposomes / Q000032:analysis / Q000276:immunology*; D008954:Models, Biological; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011817:Rabbits
D002308:Cardiolipins; D015938:Complement Membrane Attack Complex; D008081:Liposomes; D011135:Polysaccharides, Bacterial; C037676:meningococcal group C polysaccharide; D003165:Complement System Proteins