title: Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region.
abstract: Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant TiC58 plasmid and a recombinant plasmid containing either the homologous wild-type DNA region or the homologous region containing a second Tn5 insertion. The analysis identified six complementation groups. Mutations in one of these complementation groups were not complemented in trans and represent a cis-dominant locus. The mutation in one complementation group showed variation in host range.
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D009154:Mutation; D010941:Plant Tumors / Q000382:microbiology; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D013045:Species Specificity; D014774:Virulence
chemical_list: D004262:DNA Restriction Enzymes