Extracellular matrix components produced by SV40-transformed human epidermal keratinocytes.
The production of fibronectin, laminin and glycosaminoglycans (GAGs) has been investigated in normal and SV40-transformed human epidermal keratinocytes. Normal keratinocytes produced small amounts of laminin and fibronectin and three classes of GAGs; hyaluronic acid, heparan sulphate and chondroitin sulphate, hyaluronic acid being the major GAG. The SV40-transformed keratinocytes retained their cell-surface laminin and fibronectin, and in some cases showed elevated levels when compared to normal keratinocytes. All the transformants showed a shift in GAG production from hyaluronic acid to heparan sulphate, so that in all SV40-transformed keratinocytes heparan sulphate was the major GAG. These results demonstrate that the loss of extracellular matrix, which normally occurs in transformed fibroblastic cells, is not a feature of SV40-transformed human keratinocytes.
D002462:Cell Membrane / Q000378:metabolism; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002648:Child; D003470:Culture Media / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism*; D005333:Fetus; D005353:Fibronectins / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006801:Humans; D007797:Laminin / Q000302:isolation & purification; D008297:Male; D013539:Simian virus 40; D012867:Skin / Q000378:metabolism*
D003470:Culture Media; D005353:Fibronectins; D006025:Glycosaminoglycans; D007797:Laminin