Inhibitory effect of 7,8-benzoflavone on DMBA- and BaP-induced bone marrow micronuclei in mouse.
Frequencies of micronucleated polychromatic erythrocytes (PCE) were analyzed in bone-marrow cells of mice injected with 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BaP), 7,8-benzoflavone (alpha-naphthoflavone) and the combination of either 7,8-benzoflavone and DMBA or 7,8-benzoflavone and BaP. 7,8-Benzoflavone was injected 48 and 24 h before injecting mice either with DMBA or BaP. Bone-marrow samples were collected at 24, 48, 72 and 96 h. The observed maximum mean number of micronucleated PCE per 500 PCE was 8.6 at 48 h with DMBA and 11.6 at 72 h with BaP. 7,8-Benzoflavone reduced the number of micronucleated PCE in the above treatments with DMBA by 90% and in the case of BaP by 75%. In other words, 7,8-benzoflavone acted as a potent inhibitor in preventing chromosomal breaks caused by DMBA or BaP.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D001564:Benzo(a)pyrene; D001571:Benzoflavones / Q000494:pharmacology*; D001580:Benzopyrenes / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology*; D002467:Cell Nucleus / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D051379:Mice; D013997:Time Factors
D001551:Benz(a)Anthracenes; D001571:Benzoflavones; D001580:Benzopyrenes; D005419:Flavonoids; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C011512:alpha-naphthoflavone