Expression of human alpha 1-antitrypsin in Escherichia coli.
Complementary DNA coding for human alpha 1-antitrypsin has been placed under the control of the lambda PR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human alpha 1-antitrypsin (+/- 9000 molecules/cell). The product has a Mr of 44000, corresponding to mature unglycosylated alpha 1-antitrypsin.
D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D009876:Operon; D010957:Plasmids; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D006023:Glycoproteins; D000515:alpha 1-Antitrypsin