The guanine nucleotide-binding regulatory component of adenylate cyclase in human erythrocytes.
The guanine nucleotide-binding regulatory component of adenylate cyclase (G/F) has been purified from human erythrocyte membranes. It is composed of two major polypeptides with molecular weights of 35,000 and 45,000. When cyc- S49 lymphoma cell plasma membranes are reconstituted with purified human erythrocyte G/F, stimulation of adenylate cyclase by beta-adrenergic agonists, guanine nucleotides, and fluoride is restored. Binding of GTP gamma S to human erythrocyte G/F and GTP gamma S-mediated activation of the protein are closely correlated. The agreement between the apparent dissociation constants for these two reactions suggests that the measured binding site is identical to the site responsible for activation. A 41,000-dalton protein has been identified as a contaminant of preparations of G/F that have been purified by four successive chromatographic steps. This protein serves as a specific substrate for ADP-ribosylation and labeling by islet activating protein (IAP) and [32P]NAD, and it appears to contribute an additional high-affinity guanine nucleotide binding site to such preparations.
D000262:Adenylyl Cyclases / Q000097:blood*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D019204:GTP-Binding Proteins; D006150:Guanine Nucleotides / Q000097:blood; D006801:Humans; D008099:Liver / Q000201:enzymology; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D014422:Turkeys
D006150:Guanine Nucleotides; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases