Solubilization of brain alpha-2 adrenoceptor with a zwitterionic detergent: preservation of agonist binding and its sensitivity to GTP.
Alpha-2 adrenergic receptors were solubilized from calf cerebral cortex using the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate. The soluble extract retained the ability to bind the partial alpha 2 - adrenergic agonist [3H] p-aminoclonidine. This binding was of high affinity (KD = 1.5 +/- 0.3 nM) saturable (Bmax = 150 +/- 24 fmol/mg) and reversible. The orders of potencies of alpha-adrenergic drugs and nucleotides, for inhibition of binding, paralleled those previously observed on membrane bound alpha 2-adrenoceptors. Furthermore the very rapid decrease of [3H] p-amino-clonidine binding induced by GTP suggest that the guanyl nucleotide effect is due to an allosteric mechanism. These results indicate that in the soluble extract the interaction between the alpha 2-adrenoceptor and the GTP-binding regulator protein is preserved thus providing an interesting model to study the molecular structure of this system.
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002793:Cholic Acids; D003902:Detergents; D011942:Receptors, Adrenergic, alpha / Q000302:isolation & purification* / Q000378:metabolism; D012265:Ribonucleotides / Q000494:pharmacology; D012995:Solubility
D002793:Cholic Acids; D003902:Detergents; D011942:Receptors, Adrenergic, alpha; D012265:Ribonucleotides; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate