title: Identification of a large multigene family encoding the major sperm protein of Caenorhabditis elegans.
abstract: DNA fragments corresponding to genes encoding the MSP of Caenorhabditis elegans sperm have been isolated by recombinant DNA techniques. Analyses of individual genomic clones suggest that there are multiple MSP genes that are dispersed in the genome. From restriction enzyme digests of genomic DNA fractionated and hybridized with an MSP complementary DNA probe, there appear to be more than 30 MSP genes in the genome. Despite the occurrence of this large dispersed multigene family, the MSP messenger RNA from both males and hermaphrodites is homogene in size. There are at least three different proteins of identical molecular weight but different isoelectric point that cross-react with anti-MSP antisera. Each protein is a primary translation product with no detectable post-translational modifications, suggesting that at least three of the MSP genes are expressed.
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D005796:Genes; D006023:Glycoproteins / Q000032:analysis; D008297:Male; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000032:analysis; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013094:Spermatozoa / Q000032:analysis*; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D006023:Glycoproteins; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes