[Cloning of Bordetella pertussis DNA in an Escherichia coli bacterial cell system].
Hybrid plasmids containing B. pertussis DNA insertions have been constructed with the use of the vector plasmid pBR 322 and the Pst I fragments of B. pertussis DNA. Some properties of the hybrid plasmids are characterized. The possibility of the expression of B. pertussis genes in the protein-synthetizing cell-free system obtained from E. coli has been demonstrated.
D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis
D004269:DNA, Bacterial
Klonirovanie DNK Bordetella pertussis v sisteme bakterial'nykh kletok Escherichia coli.