Mouse cytochrome P3-450: complete cDNA and amino acid sequence.
A full-length cDNA clone (1,894 nucleotides) of mouse cytochrome P3-450 was isolated with the Okayama-Berg vector and sequenced. An open reading frame spanned positions 61 to 1602. The first 25, and three of the last five, amino acids of P3-450 are identical to those found in the amino- and carboxy-terminus, respectively, of the rat P-450d protein. Mouse P3-450 protein has 513 residues, and a molecular weight of 58,223 with six cysteine residues. P3-450 nucleotides 305 to 352 exhibit 74% homology, and nucleotides 1068 to 1260, 69% homology, with portions of rat P-450b exons 2 and 7, respectively. P3-450 shows 62% homology in the so-called "highly conserved region" of 39 nucleotides in the rat P-450b and P-450e and the mouse P-450b. These results indicate that P3-450, P-450b and P-450e arose from a common ancestral gene. Cysteinyl peptide-coding regions were examined: P3-450 nucleotides 1405 to 1464 exhibit 61% homology, and nucleotides 502 to 552 exhibit 37% homology, when compared with their corresponding regions in the rat P-450b gene. These data support the likelihood that cysteine 456 is the thiolate ligand to the heme iron in the P3-450 enzyme active-site.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003201:Computers; D003577:Cytochrome P-450 Enzyme System; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013045:Species Specificity
D012333:RNA, Messenger; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D004262:DNA Restriction Enzymes