Relation between intracellular sodium and twitch tension in sheep cardiac Purkinje strands exposed to cardiac glycosides.
Changes in intracellular sodium ion activity (aiNa) produced by several cardiac glycosides were correlated with twitch tension in sheep cardiac Purkinje strands. Simultaneous measurements of aiNa and twitch tension were obtained through the use of Na-sensitive intracellular microelectrodes (ETH 227) in Purkinje preparations stimulated at a frequency of 1 Hz. All concentrations of ouabain, acetylstrophanthidin, and actodigin that were tested caused an increase in aiNa immediately before, or coincident with, a positive inotropic effect. No fall in aiNa was observed at any positive inotropic concentration of digitalis in these beating fibers. In all cases, the onset and washout of the positive inotropic effect were paralleled by the rise and fall in aiNa, respectively. No dissociation between changes in aiNa and twitch tension occurred at any concentration of any of the agents used. The relation between changes in aiNa and twitch tension was linear with 1 mM increase in aiNa producing about a 100% increase in the twitch magnitude. Propranolol did not significantly alter this relationship. The increase in aiNa with digitalis was also associated with a reduction in the maximum depolarization rate of the action potential, presumably as a consequence of a reduction in the transmembrane Na electrochemical gradient. These results indicate that the positive inotropic action of digitalis in sheep Purkinje strands is always associated with a rise in aiNa secondary to inhibition of the Na pump. This increase in aiNa could increase calcium available for contraction via the Na-Ca equilibrium exchange process. In addition, the increase in aiNa reduces Vmax, as a consequence of decreasing the electrochemical gradient for Na.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002298:Cardenolides / Q000494:pharmacology; D004071:Digitalis Glycosides / Q000494:pharmacology*; D004594:Electrophysiology; D006329:Heart Conduction System / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D011690:Purkinje Fibers / Q000378:metabolism* / Q000502:physiology; D012756:Sheep; D012964:Sodium / Q000378:metabolism*; D013327:Strophanthidin / Q000031:analogs & derivatives / Q000494:pharmacology
D002298:Cardenolides; D004071:Digitalis Glycosides; D007473:Ion Channels; D010042:Ouabain; D013327:Strophanthidin; C006277:actodigin; D012964:Sodium; C004681:acetylstrophanthidin