Detection of antibodies to herpes simplex virus with a continuous cell line expressing cloned glycoprotein D.
The gene for glycoprotein D of herpes simplex virus type 1 (HSV-1) was expressed in stable mammalian cell lines. Glycoprotein D produced in these cells has a number of antigenic determinants in common with the native glycoprotein. Cell lines expressing glycoprotein D were used in an enzyme-linked immunosorbent assay to detect human antibodies to glycoprotein D. This strategy should prove useful in determining the extent to which the immune response to HSV-1 is directed toward glycoprotein D.
D000914:Antibodies, Viral / Q000032:analysis*; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C039447:glycoprotein D, Human herpesvirus 1; D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes