In vitro effects of vitamin D3 on the phospholipids of isolated renal brush border membranes.
A model system is described in which cholecalciferol (vitamin D3) is incorporated into phosphatidylcholine liposomes and then the liposomes are incubated in vitro with isolated renal brush border membrane vesicles. The incubation results in an alteration of the phospholipid composition, the fluidity, and the transport properties of the membrane. The findings provide evidence consistent with the hypothesis that vitamin D3 and metabolites modify membrane structure and function by "liponomic regulation."
D000818:Animals; D001693:Biological Transport, Active; D002762:Cholecalciferol / Q000378:metabolism / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008081:Liposomes; D008871:Microvilli / Q000187:drug effects; D010713:Phosphatidylcholines; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits; D013113:Spin Labels
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D013113:Spin Labels; D002762:Cholecalciferol; D005947:Glucose