Influence of thymic hormones on mitogen-induced interferon production in lymphocytes. II. Enhancement of Con-A-induced immune interferon production by a synthetic thymopoietin pentapeptide.
A synthetic thymic pentapeptide was used for prestimulation of mouse lymphocytes in vivo. This pentapeptide (DAc- Glu3 -TP5) affected the cAMP and cGMP levels positively and decreased the 3H-thymidine incorporation in lymphocyte cultures significantly. In combination with Con A DAc- Glu3 -TP5 influenced the production of interferon. After in vivo pretreatment of adult hybrid mice with DAc- Glu3 -TP5 the mitogen-induced interferon production was 3 to 7 times higher than in controls. The interferon produced was immune interferon (IFN gamma) as determined by pH 2-sensitivity and lack of neutralization by mouse IFN beta-antibodies.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis; D006152:Cyclic GMP / Q000096:biosynthesis; D004357:Drug Synergism; D007371:Interferon-gamma / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D013268:Stimulation, Chemical; D016305:Thymopentin; D013946:Thymopoietins / Q000494:pharmacology*; D013951:Thymus Hormones / Q000494:pharmacology*
D010446:Peptide Fragments; D013946:Thymopoietins; D013951:Thymus Hormones; D003208:Concanavalin A; D007371:Interferon-gamma; D000242:Cyclic AMP; D006152:Cyclic GMP; D016305:Thymopentin