title: Association of parainfluenza-3 virus with bovine macrophages and blood cells: an in vitro study.
abstract: Bovine blood cells and peritoneal and lung macrophages were exposed in vitro to parainfluenza-3 (PI-3) virus. Residual nonadsorbed PI-3 virus (expressed in percentage of input virus) in the supernate of the various cell fractions 1 hour after incubation at 37 C was as follows: lung macrophages, 11%; peritoneal macrophages, 59%; monocytes, 26%; RBC, 14%; lymphocytes, 28%; and polymorphonuclear cells (PMN), 63%. Lung macrophages, monocytes, lymphocytes, and PMN were monitored over a 72-hour period for hemadsorption of chicken RBC. Hemadsorption increased for lung macrophages and monocytes, whereas it decreased for lymphocytes and PMN. Infective virus could not be recovered from PMN, RBC, lymphocytes, or monocytes for more than 24 hours after PI-3 infection. Recovery of infective PI-3 virus from infected peritoneal and lung macrophages extended over 4 to 8 days, respectively.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000382:microbiology; D001773:Blood Cells / Q000382:microbiology*; D002417:Cattle; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology; D010224:Parainfluenza Virus 3, Human / Q000032:analysis / Q000302:isolation & purification*; D018108:Respirovirus / Q000302:isolation & purification*