title: The use of discontinuous Percoll gradients to separate populations of cells from human bone marrow and peripheral blood.
abstract: Cells from human bone marrow samples were separated on discontinuous Percoll gradients and the effects of variations in osmolality were studied. Yield of committed progenitor cells as determined by CFU-c and BFU-E assays was improved when a gradient of discontinuous osmolality (360-320 mosM) was compared with gradients of uniform osmolality (320 mosM and 290 mosM). The CFU-c and BFU-E containing fraction recovered from this gradient also contained fewer T cells. When used for separation of peripheral blood samples, the same gradient yielded populations of mononuclear cells, neutrophils and platelets of more than 90% purity.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001773:Blood Cells / Q000166:cytology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged; D011205:Povidone; D012822:Silicon Dioxide; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone