title: Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus.
abstract: We describe a 4.5 kilobase transposon, Tn4001, which mediates resistance to gentamicin, tobramycin and kanamycin in Staphylococcus aureus. Originally detected in plasmid pSK1, Tn4001 was shown to undergo rec-independent transposition to the chromosome from this plasmid and from an inserted derivative of the plasmid pII147. Heteroduplexes between plasmids with and without Tn4001 demonstrated a characteristic stem and loop structure with inverted repeats of approx. 1.3 kilobases.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements / Q000187:drug effects*; D004352:Drug Resistance, Microbial; D005839:Gentamicins / Q000633:toxicity*; D007612:Kanamycin / Q000633:toxicity*; D010957:Plasmids; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D014031:Tobramycin / Q000633:toxicity
chemical_list: D004251:DNA Transposable Elements; D005839:Gentamicins; D007612:Kanamycin; D004262:DNA Restriction Enzymes; D014031:Tobramycin