Inhibition of beta-adrenergic response in cultured epidermal cells by phorbol myristate acetate.
Treatment of cultured epidermal cells with the tumor promoter, phorbol myristate acetate (PMA), results in a 75% inhibition of the normal isoproterenol stimulated 7-fold increase in cAMP accumulation. Maximum inhibition occurred 3 h after PMA treatment. The effect of phorbol ester on the beta-adrenergic system in mouse epidermis thus appears to be due to its action on the epidermal cell membrane and does not require mediation by macrophages.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D010704:Phorbols / Q000633:toxicity*; D011941:Receptors, Adrenergic / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology*; D012867:Skin / Q000187:drug effects; D012879:Skin Physiological Phenomena; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
D010704:Phorbols; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP; D007545:Isoproterenol; D013755:Tetradecanoylphorbol Acetate