Translational control of the expression of the beta subunit gene of E. coli RNA polymerase.
Using the plasmid pNF1337 as template, a mRNA preparation has been obtained that directs the in vitro synthesis of fMet-Val, the N-terminal dipeptide of the beta subunit of RNA polymerase. RNA polymerase holoenzyme specifically inhibits the mRNA-directed synthesis of fMet-Val showing that the autoregulation by RNA polymerase of beta, beta 1 synthesis is at the level of translation. L factor (nusA gene product) stimulates the synthesis of fMet-Val from a DNA template but not from mRNA. Rifampicin has no effect on the mRNA-directed synthesis of fMet-Val or the ability of RNA polymerase to inhibit fMet-Val synthesis.
D001426:Bacterial Proteins / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000502:physiology; D012293:Rifampin / Q000494:pharmacology
D001426:Bacterial Proteins; D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin