Effects of deletion of the gene for the development-specific protein S on differentiation in Myxococcus xanthus.
A deletion mutation of the gene for protein S (tps), a development-specific protein of Myxococcus xanthus, was constructed. No significant differences in the process of fruiting body formation or the yield of myxospores were observed between mutant and wild-type cells. On the other hand, when the tps gene was deleted together with a 2.0-kilobase sequence including the ops gene immediately upstream of the tps gene, fruiting body formation was substantially delayed, and the yield of myxospores was reduced. These results indicate that protein S is not essential for differentiation of M. xanthus, whereas a gene product(s) coded from the sequence upstream of the tps gene appears to be required for normal fruiting body formation.
D001426:Bacterial Proteins / Q000235:genetics*; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D009231:Myxococcales / Q000235:genetics* / Q000254:growth & development; D009693:Nucleic Acid Hybridization
D001426:Bacterial Proteins; C024278:protein S, Myxococcus xanthus; D004262:DNA Restriction Enzymes