title: Origin and direction of in vitro replication of Haemophilus ducreyi and Neisseria gonorrhoeae ampicillin resistance plasmids.
abstract: The origin of replication of Haemophilus ducreyi and Neisseria gonorrhoeae ampicillin resistance plasmids was located by cloning BamHI restriction fragments into vector plasmid pAT153 and a derivative plasmid, pAT2. Selection was made for plasmid maintenance in a polA mutant. Direction of replication was determined by in vitro replication of plasmid DNA in the presence of radiolabeled deoxynucleotide.
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000096:biosynthesis; D015245:Deoxyribonuclease BamHI; D006191:Haemophilus ducreyi / Q000187:drug effects / Q000235:genetics*; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D011815:R Factors
chemical_list: D004269:DNA, Bacterial; D000667:Ampicillin; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI