A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes.
A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. The method involves virus growth in the presence of [35S]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). All of the structural proteins can be detected using this procedure, and the protein profiles of virus-adsorbed erythrocyte complexes compare extremely well with those of sucrose density gradient purified virus preparations.
D000327:Adsorption; D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000382:microbiology*; D006168:Guinea Pigs / Q000097:blood; D006387:Hemagglutination, Viral; D006801:Humans; D009980:Influenza A virus / Q000032:analysis*; D008722:Methods; D010222:Parainfluenza Virus 1, Human / Q000032:analysis; D010223:Parainfluenza Virus 2, Human / Q000032:analysis; D010224:Parainfluenza Virus 3, Human / Q000032:analysis; D018108:Respirovirus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*; D015678:Viral Structural Proteins
D014764:Viral Proteins; D015678:Viral Structural Proteins