Transformation by polyoma virus is drastically reduced by substitution of phenylalanine for tyrosine at residue 315 of middle-sized tumor antigen.
We used an oligonucleotide to introduce an A----T transversion at nucleotide position 1178 in polyoma virus DNA. The single effect of this mutation is to substitute phenylalanine for tyrosine at residue 315 of the middle-sized tumor (mT) protein (antigen). This site was previously identified as a major phosphate acceptor in the protein kinase reaction of immunocomplexes containing mT antigen. Reconstituted polyoma virus with the transversion, Py-1178-T, produces an altered mT protein that shows about 20% of the activity of wild-type mT antigen in the immunocomplex kinase assay. This residual activity appears to be directed primarily at another tyrosine at position 322 in the mT protein. The transforming ability of Py-1178-T is drastically reduced compared to wild-type virus. The efficiency of transformation by the mutant is less than 1% of that of wild type in focus assays and less than 0.1% in soft-agar growth assays. Cells identified in focus assays with Py-1178-T are generally less transformed in their phenotype than wild-type transformed cells.
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003062:Codon; D007668:Kidney; D051379:Mice; D009154:Mutation; D010649:Phenylalanine; D011120:Polyomavirus / Q000201:enzymology / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger; D014443:Tyrosine; D014764:Viral Proteins / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D003062:Codon; D012333:RNA, Messenger; D014764:Viral Proteins; D014443:Tyrosine; D010649:Phenylalanine; D011494:Protein Kinases