tRNA separation by high-performance liquid chromatography using an aggregate of ODS-Hypersil and trioctylmethylammonium chloride.
High-performance liquid chromatography on a reversed-phase support treated with a tetraalkylammonium salt was used to separate tRNAs from baker's yeast. While resolution by this column appears to result from both anion-exchange and reversed-phase chromatography, it is the hydrophobic interactions which govern the separation of one tRNA from another. Chromatography of bulk tRNA resulted in a number of fractions with different amino acid acceptor activities and little cross-contamination. In some cases the column resolved several single nucleotide modifications of tRNAPhe. Using a 250 x 6.2 mm column it has been possible to chromatograph a minimum of 100 A260 units of tRNA without serious loss in resolution. tRNAs isolated from this column as the last step of a purification procedure have very high amino acid acceptor activities.
D002851:Chromatography, High Pressure Liquid; D000644:Quaternary Ammonium Compounds; D012331:RNA, Fungal / Q000032:analysis; D012343:RNA, Transfer / Q000302:isolation & purification*; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012822:Silicon Dioxide
D000644:Quaternary Ammonium Compounds; D012331:RNA, Fungal; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; C026679:octadecylsilica; C037759:trioctylmethylammonium; D012822:Silicon Dioxide; D012343:RNA, Transfer