Erythrocyte membrane in rats fed high erucic acid-containing mustard oil: osmotic fragility, lipid composition, and (Na+, K+)- and (Ca2+, Mg2+)-ATPases.
Erythrocyte hemolytic properties, cholesterol/phospholipid ratios, fatty acid composition, and activities of the membrane-bound enzymes (Na+, K+)- and (Ca2+, Mg2+)-ATPase were studied in male and female rats fed low erucic acid rapeseed (LEAR) and high erucic acid mustard oils (HEAM) for a period of 16 months. Rats receiving groundnut oil (GNO) served as controls. Erythrocytes from HEAM-receiving male and female rats showed increased resistance to hypotonic hemolysis. In male rats this was associated with an 85% increase (P less than 0.07) in the cholesterol/phospholipid molar ratio. The fatty acid double-bond index showed an increase in male rats receiving HEAM as well as LEAR oils. In the erythrocytes from female rats, the cholesterol/phospholipid molar ratio and double bond index remained unaffected. Specific activity of ouabain-sensitive (Na+, K+)-ATPase showed a small (+20%) but significant (P less than 0.05) increase in male but not female rats of HEAM group. Total (Na+, K+)-ATPase, ouabain-insensitive component, and (Ca2+, Mg2+)-ATPase were not altered in rats receiving LEAR or HEAM.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D001937:Brassica; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004883:Erucic Acids / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000201:enzymology* / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008297:Male; D009149:Mustard Plant; D009821:Oils / Q000378:metabolism; D009996:Osmotic Fragility; D010743:Phospholipids / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism; D010938:Plant Oils; D051381:Rats; D012737:Sex Factors; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D004883:Erucic Acids; D005231:Fatty Acids, Unsaturated; D009821:Oils; D010743:Phospholipids; D010936:Plant Extracts; D010938:Plant Oils; D002784:Cholesterol; D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; C027793:mustard oil