Chronic opiate treatment does not modify alpha 2-adrenergic receptors in rat cerebral cortex, kidney and in the neurotumor cell line NCB20.
We examined the effect of chronic opiate treatment on the alpha 2-adrenergic receptor binding of both the agonist [3H]clonidine and the antagonist [3H]yohimbine in the cerebral cortex and kidney of rats. In addition, we demonstrated the presence of an adrenergic receptor of the alpha 2 type on the neurotumor cell line NCB20 and used this cell line as a model for studying the interaction between the opiate and alpha 2-adrenergic systems. In all the three above systems, chronic opiate treatment did not modify the number or the affinity of alpha 2-adrenergic receptors.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D003000:Clonidine / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D009294:Narcotics / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects*; D013997:Time Factors; D015016:Yohimbine / Q000378:metabolism
D009294:Narcotics; D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; D003000:Clonidine