Virally coded noncapsid protein associated with bovine parvovirus infection.
A phosphorylated protein (NP-1) with an Mr of 28,000 has been detected in nuclei of bovine parvovirus (BPV)-infected cells in association with chromatin. No protein in this size range was detected after infection of appropriate cells with several autonomous rodent parvoviruses although the BPV-specific protein is similar in size to noncapsid proteins associated with rabbit parvovirus or adeno-associated virus infection. Structural homology between NP-1 and a BPV capsid protein could be detected by electrophoretic analysis of the products of proteolysis with chymotrypsin. This protein can be detected after in vitro translation of RNA from BPV-infected cells and BPV-specific RNA. Homology between the in vivo- and in vitro-synthesized species was shown by the similarity of the chymotryptic products.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002460:Cell Line; D002843:Chromatin / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D010321:Parvoviridae / Q000235:genetics*; D010322:Parvoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D014176:Protein Biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D002843:Chromatin; D014764:Viral Proteins