Evidence that three adhesive proteins interact with a common recognition site on activated platelets.
Synthetic peptides corresponding to the extreme COOH terminus of the gamma chain of fibrinogen gamma 400-411, (400)HHLGGAKQAGDV(411), have been used to analyze recognition specificities of the platelet-binding sites for fibrinogen, fibronectin, and von Willebrand factor. gamma 403-411 did not inhibit 125I-fibrinogen binding to platelets. In contrast, gamma 402-411 produced dose-dependent inhibition of fibrinogen binding to ADP and thrombin-stimulated living or fixed cells and was a competitive antagonist. Inhibitory activity was not modified by addition of one (gamma 401-411) or two (gamma 400-411) histidinyl residues to the NH2 terminus, but peptides with a trifluoroacetyl group on the epsilon-amino group of lysine 406 were inactive. 125I-Fibronectin and 125I-von Willebrand factor binding to thrombin-stimulated living or fixed cells was inhibited in the same dose range by the same set of peptides which inhibited fibrinogen binding and not by gamma 403-411 or trifluoroacetate-blocked peptides. The capacity of the peptides to inhibit binding to cells with an expressed receptor, i.e. fixed cells, excludes an effect on receptor induction. Thus, these results suggest that the three adhesive glycoproteins share a common site on thrombin-activated platelets, and a 10-amino acid peptide, corresponding to gamma 402-411, defines the recognition specificity of this site.
D001779:Blood Coagulation Factors / Q000502:physiology*; D001792:Blood Platelets / Q000502:physiology*; D005340:Fibrinogen / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface / Q000502:physiology; D017469:Receptors, Fibronectin; D014841:von Willebrand Factor / Q000502:physiology*
D001779:Blood Coagulation Factors; D005353:Fibronectins; D010455:Peptides; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D017469:Receptors, Fibronectin; D014841:von Willebrand Factor; C028254:von Willebrand factor receptor; D005340:Fibrinogen