Generation of leukotrienes from human granulocytes by alveolysin from Bacillus alvei.
We investigated the effect of alveolysin on human granulocytes. Alveolysin is an exoprotein produced by Bacillus alvei and belongs to the group of sulfhydryl-activated cytolysins. Other members of this group are streptolysin O and theta-toxin from Clostridium perfringens. It is demonstrated that alveolysin leads to leukotriene generation from human granulocytes, which exert chemotactic (leukotriene B4) and slow-reacting substance (leukotriene C4, D4, and E4) activity under sublytic concentrations.
D000818:Animals; D001407:Bacillus / Q000032:analysis*; D002634:Chemotaxis, Leukocyte; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006098:Granulocytes / Q000378:metabolism*; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007975:Leukotriene B4 / Q000096:biosynthesis*; D017999:Leukotriene E4; D009930:Organic Chemicals; D013189:SRS-A / Q000031:analogs & derivatives* / Q000096:biosynthesis*
D006460:Hemolysin Proteins; D009930:Organic Chemicals; D013189:SRS-A; D007975:Leukotriene B4; D017999:Leukotriene E4; C014963:alveolysin