[Test systems for biomonitoring based on membrane-bound enzyme complexes. IV. Assessment of genotoxic effects in an Ames test system with metabolic activation by the microsomal mono-oxygenases from fish livers].
The study of mutagenic effect of 2-aminoantracene and benz(alpha)pyrene on Salmonella triphimurium TA 100 in the Ames test-system in the presence of postmitochondrial fractions S-9 from carp liver with 3-methylcholantrene induced by microsomal oxidation system has been carried out. The metabolic activity and cytochrome P450 contence in carp liver microsomes have been shown to concede considerably those in rats liver. But these characteristics are sufficient for the use of fraction S-9 from carp liver for the study of genotoxic effect of these xenobiotics in the Ames test-system. Several regimes of storage of S-9 preparations from carp liver have been compared. S-9 preparations frozen immediately after isolation preserve their metabolic activity with respect to 2-aminoantracene and benz(alpha)pyrene well.
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000494:pharmacology; D002347:Carps; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005796:Genes / Q000187:drug effects*; D005798:Genes, Bacterial / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000494:pharmacology; D010105:Oxygenases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects
D000873:Anthracenes; D001580:Benzopyrenes; D009153:Mutagens; D001564:Benzo(a)pyrene; C006593:2-anthramine; D010105:Oxygenases; D004121:Dimethyl Sulfoxide
Test-sistemy dlia biomonitoringa na osnove membranno-sviazannykh fermentnykh kompleksov. IV. Otsenka genotokischeskikh éffektov v testsisteme Eĭmsa s metabolicheskoĭ aktivatsieĭ mikrosomal'nymi monooksigenazami iz pecheni ryb.