Inhibitor-1 phosphatase activity in vascular smooth muscle.
The histone-H1 and polylysine stimulated "latent" phosphorylase phosphatase, characterized by a molecular weight of 260,000 in gel filtration and 130,000 in sucrose density gradient centrifugation has been identified as a major inhibitor-1 phosphatase in vascular smooth muscle. Its substrate: protein phosphatase inhibitor-1, was also shown to be present in the same tissue. Following treatment with beta-mercaptoethanol the enzyme dissociates into a lower molecular weight (38,000) form with a higher specific activity.
D000818:Animals; D001011:Aorta / Q000201:enzymology; D002352:Carrier Proteins; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D000242:Cyclic AMP / Q000494:pharmacology; D006657:Histones / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010765:Phosphorylase Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C021042:protein phosphatase inhibitor-1; D008623:Mercaptoethanol; D000242:Cyclic AMP; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D010765:Phosphorylase Phosphatase