Expression of the human interferon-beta gene cloned in phage M13 mp7.
The single-stranded DNA phage, M13 mp7 was used in the construction of an expression vector containing the coding sequence for mature interferon-beta (IFN-beta). Two clones expressed a fused polypeptide showing the biological and physicochemical properties of IFN-beta, despite the fact that the N-terminal amino acid sequence had been changed; 10(6) I.U./l of culture were produced with a molecular weight of 20 000.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008970:Molecular Weight
D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D007371:Interferon-gamma; D004262:DNA Restriction Enzymes