Inhibition of herpes simplex virus DNA synthesis by pokeweed antiviral protein.
Pokeweed antiviral protein at a concentration of 3 microM inhibited both the synthesis and release of infectious herpes simplex virus type 1 in cell culture by 90 and 99%, respectively. Addition of pokeweed antiviral protein to Vero cell monolayers before virus infection was 10 to 15% more effective in reducing virus yields than was the simultaneous addition of the antiviral protein with virus inoculum. Viral DNA synthesis was inhibited by 90% in cells which had been exposed to the antiviral protein, whereas cellular DNA synthesis was unaffected. No significant inhibition in the synthesis of the majority of viral infected-cell polypeptides was observed early postinfection (7 h), with the exception of infected cell polypeptides 4 and 41, whose syntheses were reduced by 38 and 25%, respectively. At 9 to 21 h postinfection, however, the synthesis of individual infected cell polypeptides was reduced by 48 to greater than 99%.
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis*; D000882:Haplorhini; D007700:Kinetics; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000494:pharmacology*; D054789:Ribosome Inactivating Proteins, Type 1; D018139:Simplexvirus / Q000187:drug effects* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
D000998:Antiviral Agents; D004279:DNA, Viral; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D014764:Viral Proteins; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein