title: Studies on the structure and organization of avian sarcoma proviruses in the rat XC cell line.
abstract: The structure and arrangement of the multiple provirus copies of avian sarcoma virus in a rat XC cell line were studied by restriction endonucleases. The following observations were made: (i) the majority of the proviruses integrated randomly with respect to cell DNA; (ii) no gross deletions or rearrangements in the proviruses were observed; (iii) two types of proviruses (type I and type II) could be distinguished on the basis of restriction endonuclease cleavage sites; (iv) the virus rescued from these cells was derived from type II provirus, which has a novel EcoRI site between the env and pol genes; (v) most of the provirus units contained the src gene.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000648:ultrastructure*; D002460:Cell Line; D002642:Chick Embryo; D003434:Crossing Over, Genetic; D004247:DNA / Q000032:analysis / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005354:Fibrosarcoma / Q000382:microbiology; D005814:Genes, Viral; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes