Restriction endonuclease map of corynebacteriophage omega ctox+ isolated from the Park-Williams no. 8 strain of Corynebacterium diphtheriae.
The toxigenic corynebacteriophage omega tox+ was isolated from the hypertoxigenic Park-Williams no. 8 (PW8) strain of Corynebacterium diphtheriae and compared with the toxigenic corynebacteriophage beta tox+. The physical size and host range of both phages were found to be identical. An endonuclease restriction map of omega tox+ was constructed, and the locations of the cohesive ends (cos), phage attachment site (attP), and the diphtheria tox operon were identified. The genome of omega tox+ was found to differ from that of beta tox+ in three regions. In addition, omega tox+ was shown to be integrated into two nontandem corynebacterial phage attachment sites (attB1, attB2) in the PW8 chromosome. The differences in the restriction endonuclease digestion maps of omega tox+ and beta tox+ and the contribution of double lysogeny are discussed in relation to the hypertoxigenicity of the PW8 strain.
D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D003353:Corynebacterium diphtheriae; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D004167:Diphtheria Toxin / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D008242:Lysogeny
D004279:DNA, Viral; D004167:Diphtheria Toxin; D004262:DNA Restriction Enzymes