Bluetongue virus: comparative evaluation of enzyme-linked immunosorbent assay, immunodiffusion, and serum neutralization for detection of viral antibodies.
Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay results. However, the immunodiffusion method failed to detect bluetongue virus antibody in a substantial number of sera found to possess bluetongue virus immunoglobulin G with the enzyme-linked immunosorbent assay.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001820:Bluetongue virus / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007163:Immunosorbent Techniques; D012087:Reoviridae / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G