Solitary horizontal cells in culture--I. Their electrical properties.
Membrane properties of solitary horizontal cells were examined under current- and voltage-clamp conditions. The resting membrane potential was mainly determined by K ions. The cells produced all-or-none action potentials and showed non-linear current-voltage relationships with inward- and outward-going rectification. Pharmacological and ion-substitution experiments under voltage-clamp conditions revealed that the ionic current was separated into at least four components; a Ca current and three types of K currents (a K current through an anomalous rectifier, a transient outward current, and a maintained outward current). The action potentials and the non-linear current-voltage relationships of solitary horizontal cells were explained from these ionic currents.
D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004553:Electric Conductivity; D006054:Goldfish; D007473:Ion Channels; D008564:Membrane Potentials; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D012160:Retina / Q000166:cytology*
D007473:Ion Channels; D011188:Potassium; D002118:Calcium