title: Differential pulse voltammetric determination of 5-hydroxyindoles in four raphe nuclei of chronic freely moving rats simultaneously recorded by polygraphic technique: physiological changes with vigilance states.
abstract: Nuclei raphe dorsalis ( RDN ), centralis (RCN), pontis (RPN) and magnus ( RMN ) were separately studied using differential pulse voltammetry ( DPV ) in chronic freely moving rats during the recording of their sleep-waking cycle by polygraphic technique. In each of these nuclei the height of the electrochemical signal appearing at +300 mV (peak 3) was maximum during waking (W), lower during slow-wave sleep (SWS) and minimum during paradoxical sleep (PS). Some pharmacological treatments indicated that in each of these nuclei the peak 3 represents the oxidation of the 5-hydroxyindoles. DPV measurements performed during specific behavioral states (eating, grooming, washing, drinking) called active waking (AW) or manipulations (handling, tail-pinch) demonstrated that this technique enables detection of changes occurring in animals under physiological conditions.
mesh_terms: D000818:Animals; D001143:Arousal / Q000502:physiology*; D001933:Brain Stem / Q000502:physiology*; D008297:Male; D011903:Raphe Nuclei / Q000502:physiology*; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D012894:Sleep Stages / Q000502:physiology; D009435:Synaptic Transmission; D014851:Wakefulness / Q000502:physiology
chemical_list: D012701:Serotonin