Antibody to a synthetic nonapeptide corresponding to the NH2 terminus of poliovirus genome-linked protein VPg reacts with native VPg and inhibits in vitro replication of poliovirus RNA.
A synthetic nonapeptide corresponding to the N-terminal sequence of poliovirus genome-linked protein (VPg) was linked to bovine serum albumin and used to raise antibodies in rabbits. The antipeptide antibodies specifically precipitated the nonapeptide, native VPg, and VPg-linked poliovirion RNA. The antipeptide antibodies inhibited host factor-stimulated, poliovirus replicase-catalyzed in vitro synthesis of full-length (35S) RNA in response to virion RNA. Oligouridylic acid-stimulated RNA synthesis was not affected by the antipeptide antibodies. Preincubation of the antibodies with excess nonapeptide reversed the antipeptide antibody-mediated inhibition of host factor-stimulated RNA synthesis by the poliovirus replicase. A role for VPg in the in vitro replication of poliovirus RNA genome is discussed.
D000914:Antibodies, Viral; D000937:Antigen-Antibody Reactions; D002352:Carrier Proteins / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D034982:Integration Host Factors; D009843:Oligoribonucleotides / Q000494:pharmacology; D010455:Peptides / Q000276:immunology; D017955:Poliovirus / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors; D014500:Uracil Nucleotides / Q000494:pharmacology; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000276:immunology / Q000502:physiology*
D000914:Antibodies, Viral; D002352:Carrier Proteins; D034982:Integration Host Factors; D009843:Oligoribonucleotides; D010455:Peptides; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014758:Viral Core Proteins; D014764:Viral Proteins; C027916:oligo(U); D012324:RNA-Dependent RNA Polymerase