title: Phenotypic changes in the flavivirus Kunjin after a single cycle of growth in an Aedes albopictus cell line.
abstract: The properties of Kunjin virus produced during acute infections of Aedes albopictus (Aal) mosquito cells were compared with those of the virus progeny from the C6/36 clone of mosquito cells and from Vero cells. Titres of 10(8) p.f.u./ml or greater were obtained from all cells, but significant haemagglutinin activity was associated only with progeny from Vero and C6/36 cells. Kunjin virus from Aal cells adsorbed to goose erythrocytes and blocked haemagglutination by virus from Vero or C6/36 cells. High titres of virus from Aal cells were obtained only when the cells were grown in the presence of 10% or higher concentrations of foetal calf serum, and large losses were encountered when this virus was concentrated by pelleting or by precipitation with polyethylene glycol, and during rate-zonal sedimentation through sucrose gradients. Because of these losses, and because of poor incorporation of [35S]methionine in Aal cells, only analytical amounts of labelled structural proteins were recovered. Electropherograms showed that the presumptive envelope protein E of Kunjin virus from Aal cells migrated more rapidly than E of virions grown in C6/36 and Vero cells, and that the core (C) and membrane-like (M) proteins were not detectably labelled. All the observed changes in phenotype were reversed during one cycle of growth in Vero cells.
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005416:Flavivirus / Q000302:isolation & purification / Q000502:physiology*; D006387:Hemagglutination, Viral; D010641:Phenotype; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000032:analysis; D015678:Viral Structural Proteins; D014779:Virus Replication
chemical_list: D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins