Lymphocyte transformation test in diagnosis of nickel allergy. A comparison between the separation of peripheral blood lymphocytes on Ficoll-Paque, Percoll or by gravity sedimentation.
Different lymphocyte separation procedures, Percoll and Ficoll-Paque density sedimentation and gravity sedimentation, were tested in order to get optimal results in the lymphocyte transformation test in diagnosis of nickel allergy. Percoll at both investigated times, 3 and 6 days, gave the highest lymphocyte DNA synthesis response to nickel sulfate in nickel-allergic subjects and the most significant difference against control subjects. With all tested separation procedures there was an overlap between the patient and control group as regards the individual responses.
D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D005362:Ficoll / Q000494:pharmacology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000175:diagnosis / Q000276:immunology*; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D009532:Nickel / Q000008:administration & dosage / Q000276:immunology*; D011205:Povidone / Q000494:pharmacology; D012822:Silicon Dioxide / Q000494:pharmacology
D037102:Lectins; D005362:Ficoll; C029938:nickel sulfate; C016039:Percoll; D012822:Silicon Dioxide; D009532:Nickel; D004247:DNA; D011205:Povidone