title: Transcription analysis of the lexA gene of Escherichia coli: attenuation and cotranscription with the neighboring region.
abstract: The lexA gene of Escherichia coli encodes a repressor of the genes whose expression is induced by the agents that result in DNA damage. In vivo transcripts of the lexA gene consisted of two species; mRNA-1 of 673 bases and mRNA-2 of approximately 3,000 bases. The transcription in vivo started at a site which was two-base pairs downstream from the in vitro initiation site reported previously. The majority of the transcription stopped at a series of T residues preceeded by a dyad symmetry located immediately after the lexA gene. A small fraction of the transcription passed through the termination site to form the mRNA of downstream gene(s) which would be related to the "SOS functions".
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D009876:Operon; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics*; D012697:Serine Endopeptidases; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099160:LexA Repressor Protein