title: JC virus T protein expression in owl monkey tumor cell lines.
abstract: Four owl monkey tumor cell lines were tested for the presence of JCV early gene products. Owl 26 was the only cell line in which large T protein could be immunoprecipitated using cross-reactive anti-SV40 hamster tumor sera. Small t protein could not be detected in any of the four cell lines. The monoclonal antibody Cl 122, directed against the host-cell 53K protein, could bind the SV40-T-53K host protein complex in human embryonic kidney cells transformed by SV40. However, the Cl 122 antibody only reacted with a 53K protein in Owl 26 not complexed with the JCV T protein.
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000956:Antigens, Viral / Q000032:analysis; D000957:Antigens, Viral, Tumor; D001029:Aotus trivirgatus; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D007577:JC Virus / Q000032:analysis*; D011120:Polyomavirus / Q000032:analysis*; D011233:Precipitin Tests; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins