The NADPH-dependent O-.2-generating oxidase from human neutrophils.
A subcellular particulate fraction from normal neutrophils that was enriched in NADPH-dependent O-.2-generating activity (Gabig, T. G., Schervish, E. W., and Santinga, J. T. (1982) J. Biol. Chem. 257, 4114-4119) has been further characterized. This preparation contained 0.25 +/- 0.02 nmol of flavin adenine dinucleotide/mg of protein and 0.28 +/- 0.01 nmol of cytochrome b/mg of protein. Measurable amounts of riboflavin or flavin mononucleotide were not present. The flavoprotein was completely resolved from the cytochrome b by selective bile salt extraction of the particulate oxidase fraction. The identical subcellular particulate fraction was studied in the neutrophils from two male patients with chronic granulomatous disease. The neutrophil oxidase fraction from one of the chronic granulomatous disease patients had a cytochrome b component that was spectrally abnormal, but a normal content of flavin adenine dinucleotide. The fraction from this patient's neutrophils corresponding to the resolved flavoprotein from normal cells had fluorescence excitation and emission spectra that were identical to the normal flavoprotein. The neutrophil oxidase fraction from the second chronic granulomatous disease patient had a quantitatively and spectrally normal cytochrome b but less than 8% of the normal amount of flavin adenine dinucleotide. The fraction from the latter patient's neutrophils corresponding to the resolved flavoprotein from normal cells had no detectable flavoprotein by fluorescence excitation and emission spectroscopy. It is postulated that these two patients represent distinct mutants in two separate components of the neutrophil NADPH-dependent O-.2-generating oxidase system, flavoprotein and cytochrome b.
D003573:Cytochrome b Group / Q000097:blood; D005182:Flavin-Adenine Dinucleotide / Q000097:blood; D005420:Flavoproteins / Q000097:blood*; D006105:Granulomatous Disease, Chronic / Q000097:blood*; D006801:Humans; D008297:Male; D009249:NADP / Q000494:pharmacology*; D009504:Neutrophils / Q000201:enzymology*; D010088:Oxidoreductases / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013481:Superoxides / Q000097:blood
D003573:Cytochrome b Group; D005420:Flavoproteins; D013481:Superoxides; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D010088:Oxidoreductases