title: The immediate-early mRNA that encodes the regulatory polypeptide Vmw 175 of herpes simplex virus type 1 is unspliced.
abstract: The structure and precise map location of the 4.1-kb herpes simplex virus type 1 (HSV-1) immediate-early mRNA (IE mRNA-3) that encodes a regulatory polypeptide, with an approximate mol. wt. of 175 000, has been determined. Nuclease S1 digestion procedures using labelled virus DNA probes have shown that IE mRNA-3 is unspliced and maps entirely within the TRs/IRs genome regions. DNA sequences at the 5' and 3' ends of IE mRNA-3 have been examined and regulatory signals involved in initiation of transcription and polyadenylation have been identified. No other mRNA is known to map within the region coding for IE mRNA-3.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D007668:Kidney; D051379:Mice; D009693:Nucleic Acid Hybridization; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes