Tissue specificity of human phosphodiesterase. II. Intestinal mucosa.
Extracts of human intestinal mucosa were examined for their ability to hydrolyze various phosphodiester, phosphomonoester and phenylphosphonate ester linkages. Enzymes carrying out these reactions were partially purified by butanol extraction, ammonium sulfate precipitation and DEAE-cellulose chromatography, and examined for polymorphism on polyacrylamide gels. Two species of alkaline phosphatase and at least five species of PDE I were identified. Antibodies to purified bovine intestinal phosphatase and phosphodiesterase were found specific for the respective human enzymes.
D000469:Alkaline Phosphatase / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000302:isolation & purification*; D011110:Polymorphism, Genetic
D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I