[125I]LDL uptake in rabbit arteries perfused in situ. Effect of HDL on intact and de-endothelialized vessels.
The uptake of [125I]LDL by rabbit abdominal and thoracic aortae, and carotid arteries perfused in situ was studied. [125I]LDL uptake in vessels with intact endothelial covering was saturable, Ca2+, Mg2+-dependent and was inhibited by a 100-fold excess of LDL and HDL, on the average by 30% and 60%, respectively. In de-endothelialized vessels [125I]LDL uptake increased by approximately 3-fold, and was not reduced in the presence of excess of unlabeled LDL and HDL.
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002339:Carotid Arteries / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism; D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010477:Perfusion; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism; D011973:Receptors, LDL
D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011956:Receptors, Cell Surface; D011973:Receptors, LDL