Estradiol-17 beta inhibition of lutropin and dibutyryl cyclic AMP induced steroidogenesis in intact bovine luteal cells. Absence of effect on induced protein phosphorylations.
Estradiol-17 beta is known to inhibit in a dose dependent manner the lutropin-induced stimulation of progesterone synthesis in luteal cells without affecting the intracellular cyclic AMP increase produced by the hormone. The hypothesis that this inhibitory action could involve an inhibition of the cyclic AMP dependent phosphorylation of cytosolic proteins was investigated by using incubations of selected small bovine luteal cells. Doses of 10 and 100 micrograms/ml of estradiol-17 beta inhibited respectively 60 and 90% of the progesterone synthesis induced by lutropin as well as by dibutyryl cyclic AMP in small bovine luteal cells. At the concentrations of 10 and 100 micrograms/ml, estradiol-17 beta was unable to affect the cyclic AMP dependent protein kinase activation induced by lutropin. At the concentration of 10 micrograms/ml the steroid was without effect on the lutropin or dibutyryl cyclic AMP induced protein phosphorylations. However 100 micrograms/ml of estradiol-17 beta seemed to produce a slight inhibition of the induced protein phosphorylations.
D000818:Animals; D003994:Bucladesine / Q000037:antagonists & inhibitors*; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors*; D010750:Phosphoproteins / Q000096:biosynthesis*; D010766:Phosphorylation; D011374:Progesterone / Q000096:biosynthesis*; D011494:Protein Kinases / Q000378:metabolism
D010750:Phosphoproteins; D011374:Progesterone; D004958:Estradiol; D003994:Bucladesine; D007986:Luteinizing Hormone; D000242:Cyclic AMP; D011494:Protein Kinases