title: A note on the ADP-ribose-protein linkages in rat-liver nuclei: a possible approach to assessing megavitamin therapy with niacin.
abstract: Nuclei isolated from rat liver were incubated with NAD whose two ribose moieties were respectively labeled with 3H or 14C. By enzymatic (phosphodiesterase) and/or chemical (hydroxylamine) attack on doubly labeled ADP-ribosylated nuclear residues, AMP was found after hydroxylaminolysis as well as iso-ADP-ribose after phosphodiesterase plus hydroxylamine, in the absence of detectable amounts of ribose-5-phosphate. This is taken to indicate the existence of additional ribose-protein binding sites in in vitro ADP-ribosylated nuclear proteins: Besides C-1" (Hayaishi et al., Stocken et al.) C-2' and/or C-3' (purine-near) as well as C-2" and/or C-3" (pyrimidine-near), not only at the end but also within the chain of oligo-ADPR.
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D008099:Liver / Q000648:ultrastructure; D009243:NAD / Q000378:metabolism; D009525:Niacin / Q000627:therapeutic use; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D009974:Orthomolecular Therapy; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: D006898:Hydroxylamines; D009702:Nucleoside Diphosphate Sugars; D011506:Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D009525:Niacin; D019811:Hydroxylamine; D010727:Phosphoric Diester Hydrolases