Low levels of RNase H activity in Escherichia coli FB2 rnh result from a single-base change in the structural gene of RNase H.
The DNA coding for RNase H from a mutant strain of Escherichia coli (FB2) was cloned into plasmid pBR322. DNA sequence analysis and the exchange of a portion of the mutant and wild-type genes revealed that a single-base alteration (C-->T) in the coding region of the structural gene for RNase H is responsible for the difference in RNase H activity of the wild-type and mutant cells.
D001483:Base Sequence; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D011487:Protein Conformation; D016914:Ribonuclease H
D004722:Endoribonucleases; D016914:Ribonuclease H