[Radiometric method of determining ATP: D-pantothenate-4'-phosphotransferase activity].
A radiometric procedure is developed for estimation of pantothenate kinase (EC 2.7.1.33) activity in various preparations of rat liver tissue; sodium 14C-D-pantothenate was used as a substrate and the reaction end product 4'-phosphopantothenic acid was measured. Optimal separation of the substrate and the end product was achieved by means of chromatography on DEAE-Sephadex A-25. 4'-phosphopantothenic acid was eluted from the column by 0.4 N HCl thus avoiding the label dilution and possible quenching of scintillation.
D000818:Animals; D002852:Chromatography, Ion Exchange; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011874:Radiometry; D051381:Rats
D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C023088:pantothenate kinase
Radiometricheskiĭ metod opredeleniia aktivnosti ATP: D-pantotenat-4'-fosfotransferazy.