Species differences in the properties of mammalian transcobalamin II.
Striking differences in physical and immunologic properties of transcobalamin II (TC II) in six mammalian species were noted. Polyacrylamide gel electrophoresis of TC II suggested the presence of isoproteins in several species. A microfine precipitate of silica (Quso), adsorbed TC II directly only from human and canine plasma. TC II in some species appears to be associated with a high molecular weight constituent of plasma, resulting in the TC II being unavailable to bind to Quso. Quso should therefore not be used to assay TC II in the plasma of all species without prior validation.
D000327:Adsorption; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012822:Silicon Dioxide; D013045:Species Specificity; D014155:Transcobalamins / Q000032:analysis*
D014155:Transcobalamins; D012822:Silicon Dioxide