Histological and histochemical characterisation of mammary gland tissue of cows infected with foot-and-mouth disease by contact exposure.
Foot-and-mouth disease virus was observed to replicate in secretory epithelial cells of bovine mammary gland alveoli as a result of systemic infection initiated by exposure to infected animals. Viral antigens were demonstrated using fluorescent antibody and immunoperoxidase labelling techniques before the development of signs of clinical disease. In addition, labelled antigens were observed associated with cytoplasmic-like fragments in luminal membrane limited structures. Histologically, lesions of the alveolar secretory epithelium consisted of focal necrosis of these cells which eventually sloughed into the lumen.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005537:Aphthovirus / Q000276:immunology / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005536:Foot-and-Mouth Disease / Q000382:microbiology / Q000473:pathology*; D007124:Immunoenzyme Techniques; D008321:Mammary Glands, Animal / Q000473:pathology*
D000956:Antigens, Viral