The rapid H2O2-mediated nonphotodynamic crosslinking of lens crystallins generated by the heme-undecapeptide from cytochrome C: potential implications for cataractogenesis in man.
Human cataract lens crystallins are crosslinked and demonstrate a non-tryptophan blue fluorescence. We report here that exposure of lens crystallin to H2O2 within the concentration range reported for human aqueous humor, produces crosslinking of crystallin polypeptides within 10 minutes in the presence of the heme-undecapeptide from cytochrome c. Concomitantly, a blue fluorescence develops. These findings suggest the possibility that under some conditions hydrogen peroxide activation may play a role in cataractogenesis in vivo.
D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000378:metabolism; D002417:Cattle; D003432:Cross-Linking Reagents; D003459:Crystallins / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000502:physiology*; D010455:Peptides / Q000378:metabolism*
D003432:Cross-Linking Reagents; D003459:Crystallins; D003574:Cytochrome c Group; D006420:Hemeproteins; D010455:Peptides; D006861:Hydrogen Peroxide