title: Structure of the chloroplast gene for the precursor of the Mr 32,000 photosystem II protein from mustard (Sinapis alba L.).
abstract: The nucleotide sequence of the mustard chloroplast gene for the precursor of the Mr 32,000 photosystem II protein is presented. A comparison with the corresponding genes from spinach and Nicotiana debneyi (14) reveals less than 5% nucleotide divergence in the coding region. The derived protein of mustard differs from the corresponding proteins by three amino acid positions at the C-terminus. We have defined the presumed transcription start and termination sites of the mustard gene. Upstream from the start site are sequences typical of a prokaryotic promoter and, also, a sequence that resembles the eukaryotic 'TATA' box. A search for intrastrand base pairing revealed stem-loop secondary structure at the transcription start and termination sites and in the region preceding the presumed promoter. This latter region is a 69-base-pair sequence element unique to the 5'flanking sequence of the mustard gene.
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005796:Genes; D008970:Molecular Weight; D009149:Mustard Plant / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000378:metabolism*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011498:Protein Precursors / Q000235:genetics*; D013045:Species Specificity; D014026:Nicotiana / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; D004262:DNA Restriction Enzymes