Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis.
Bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mM-NaIO4 at 0 degree C, liberating C-9 as formaldehyde which was further quantitated using 3-methyl-2-benzothiazolinone. The mean +/- s.d. (n = 20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50.9 +/- 8.0 and 25.2 +/- 3.8 nmol/10(8) spermatozoa respectively. About 85% of the former and 75% of the latter could be extracted by 1% Triton X-100 and 2 mM-dithiothreitol. About 70% of the former and 20% of the latter were released by neuraminidase from Vibrio cholerae. About 40% of the former and 30% of the latter were sensitive to trypsin. During sperm maturation, the decrease in the total bound sialic acids was due to the decrease in the neuraminidase-sensitive but not the neuraminidase-resistant sialic acids.
D000818:Animals; D004229:Dithiothreitol; D004822:Epididymis; D008297:Male; D008722:Methods; D009439:Neuraminidase; D010504:Periodic Acid / Q000494:pharmacology; D011092:Polyethylene Glycols; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*; D014357:Trypsin
D012794:Sialic Acids; D010504:Periodic Acid; D011092:Polyethylene Glycols; C009288:metaperiodate; D009439:Neuraminidase; D014357:Trypsin; D004229:Dithiothreitol