Monitoring of carbohydrates with periodate in effluents from high-pressure liquid chromatography columns.
Application of periodate to monitoring of carbohydrates in effluents from borate anion-exchange columns is described. The effect of temperature to speed the reaction in a postcolumn reactor was investigated at pH 5.0 and 8.6. The alkaline conditions increase sensitivity for some carbohydrates due to overoxidation. Temperatures up to 100 degrees C may be employed to detect less reactive sugars such as cyclitols. Peaks are detected by an absorbance decrease at 260 nm with a high-pressure liquid chromatography detector. The reagent permits detection of a wide variety of carbohydrates, aldoses, ketoses, alditols, cyclitols, and oligosaccharides with sensitivities of less than 1 nmol.
D002241:Carbohydrates / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010504:Periodic Acid / Q000032:analysis; D013025:Glycine max / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013402:Sugar Alcohols / Q000302:isolation & purification; D013696:Temperature
D002241:Carbohydrates; D006023:Glycoproteins; D013402:Sugar Alcohols; D010504:Periodic Acid