Male sexual development in the nonhuman primate. III. Sertoli cell culture and age-related differences.
Homogenous preparations of primary Sertoli cell cultures were obtained from the testes of the macaque of different ages. The characteristics of Sertoli cells were confirmed by electron microscopy. Sertoli cell cultures were segregated into three developmental age groups: prepubertal, pubertal, and adult. The highest response to follicle-stimulating hormone [FSH (NIH-FSH-S13) as measured by cAMP and testosterone to estradiol conversion occurred in Sertoli cells from pubertal animals, whereas the responses were diminished in cells from both younger and older animals. Specific binding of 125I-human FSH was also increased in Sertoli cells prepared from pubertal animals when compared to cells from the other two age groups. These data demonstrate: 1) the utility of primate Sertoli cells as an in vitro model, and 2) the age-related differences in monkey Sertoli cell response to FSH and to specific FSH receptor binding.
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D012741:Sexual Maturation; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP