Genetic evidence that action of cAMP-dependent protein kinase is not an obligatory step for antiviral and antiproliferative effects of human interferon in Chinese hamster ovary cells.
Several Chinese hamster ovary (CHO) cell mutants with altered cAMP-dependent protein kinases were utilized to study the possible involvement of this enzyme in mediating interferon effects in cultured cells. Human fibroblast interferon had a pronounced protective effect for CHO cells against encephalomyocarditis virus (EMCV). This protective effect was also observed in cAMP-resistant CHO mutants with protein kinase defects, although to a slightly lesser extent. EMCV, however, which grows well in wild-type CHO cells, grew poorly in the cAMP-resistant CHO mutants even in the absence of interferon, suggesting that cAMP-dependent protein kinase is involved in the normal growth of this virus. The growth inhibitory and morphological effects of human fibroblast interferon on CHO cells as well as the induction of 2',5'-oligoA synthetase and the level of RNase F activity (which is constitutive in CHO cells) were very similar in wild-type and mutant CHO cells. It is concluded from these studies that cAMP-dependent protein kinase may have a facilitating role in antiviral activity of interferon, but does not have an obligatory role in mediating either the antiviral or antiproliferative effects of interferon in CHO cells.
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000502:physiology; D004722:Endoribonucleases / Q000378:metabolism; D004790:Enzyme Induction; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D009154:Mutation; D010053:Ovary; D011494:Protein Kinases / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014779:Virus Replication
D007370:Interferon Type I; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase; D004722:Endoribonucleases; C021885:ribonuclease F