Maintenance of neonatal endocrine cells of the rat. I. Effects of 2-deoxyglucose and 2-deoxy-2-fluoroglucose.
Pancreatic endocrine cells of the neonatal rat cultured in medium with 5.5 mM glucose for 7 days showed no response to glucose. By contrast, the supplementation of the medium with 1.0 mM 2-deoxyglucose or with 0.1-5.0 mM 2-deoxy-2-fluoroglucose maintained the capacity of glucose-induced insulin release and biosynthesis, and the recovery of insulin in cells at day 7 at levels significantly higher than in basal medium; the highest responses were recorded for 1.0 mM deoxysugars. Moreover, the addition of 1.0 mM deoxysugars caused a selective deletion of fibroblasts and yielded monolayers mostly consisted of endocrine cells at the end of the culture study period. In these monolayer cells, the stimulating level of c-AMP release was significantly higher than the basal. On the other hand, the in vitro function of A cells in culture was also better preserved in media with 1.0 mM deoxysugars.
D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D019788:Fluorodeoxyglucose F18; D005934:Glucagon / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology; D008858:Microscopy, Phase-Contrast; D051381:Rats; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media; D003837:Deoxy Sugars; D007328:Insulin; D019788:Fluorodeoxyglucose F18; D005934:Glucagon; D003847:Deoxyglucose; D000242:Cyclic AMP