An opsonised electrode. The direct electrochemical detection of superoxide generated by human neutrophils.
A pyrolytic graphite electrode was surface modified with human IgG and used as a stimulus to elicit a respiratory burst from human neutrophils. The oxidation current observed was shown to be due to re-oxidation of superoxide produced by the neutrophils. Both superoxide dismutase and N-ethylmaleimide were effective inhibitors of the oxidation current.
D004563:Electrochemistry; D004566:Electrodes; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D009504:Neutrophils / Q000032:analysis*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000097:blood*
D007074:Immunoglobulin G; D013481:Superoxides; D013482:Superoxide Dismutase; D005033:Ethylmaleimide