title: Comparative immunocytochemical demonstration of ACTH-, LH- and FSH-containing cells in the pituitary of neonatal, immature and adult rats.
abstract: By means of immunocytochemistry, the development of ACTH-, LH- and FSH cells was examined in the anterior pituitary of 5-day-old neonatal, 15-day-old immature and adult rats. ACTH-positive cells are angular and the periphery of these cells is strongly reactive with anti-ACTH serum. In contrast, LH- and FSH-immunopositive cells are ovoid elements, ranging in cell size and intensity of staining. Angular cells, in which only the cell periphery reacted with anti-LH beta serum, were observed in neonatal and immature rats; however, these cells were not stained with either anti-FSH beta serum or anti-ACTH serum. Observation of serial semithin sections revealed that ACTH-immunopositive cells do not react with either anti-LH beta or anti-FSH beta serum. Finally, it was observed that ACTH cells and LH cells are both functionally differentiated already in 5-day-old neonatal rats.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000032:analysis*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000166:cytology* / Q000254:growth & development; D051381:Rats
chemical_list: D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone