Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.
A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'-monophosphate carboxylase in E. coli is within the Neurospora sequence.
D002262:Carboxy-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D014170:Transformation, Genetic
D004262:DNA Restriction Enzymes; D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase