Purification and characterization of ubiquitin from mammalian testis.
Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved methods for radioimmunoassay of ubiquitin in tissue extracts are also discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D008297:Male; D008322:Mammals; D009698:Nucleoproteins / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011863:Radioimmunoassay; D013737:Testis / Q000032:analysis*; D014452:Ubiquitins
D000596:Amino Acids; D002868:Chromosomal Proteins, Non-Histone; D009698:Nucleoproteins; D014452:Ubiquitins