Effect of cortisol on cyclic AMP production stimulated by 1-24 ACTH in adrenal cortex membranes.
The effect of cortisol on cyclic AMP production by crude bovine adrenal cortex membrane preparations has been investigated. Results demonstrate that in the presence of cortisol, cyclic AMP production in response to 1-24 ACTH was enhanced up to a concentration of about 74 microM cortisol. At higher concentrations the effect was reversed. Cortisol had no effect on cyclic AMP production in the absence of 1-24 ACTH, and cyclic AMP production was completely inhibited when 5 mM EDTA was added to the incubation tubes with the cortisol.
D000302:Adrenal Cortex / Q000378:metabolism / Q000648:ultrastructure*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives*; D000818:Animals; D002417:Cattle; D003366:Cosyntropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology*; D008566:Membranes / Q000378:metabolism
D003366:Cosyntropin; D000324:Adrenocorticotropic Hormone; D004492:Edetic Acid; D000242:Cyclic AMP; D006854:Hydrocortisone