title: Accumulation of cyclic GMP in filaments of Escherichia coli BUG6.
abstract: Experiments with Escherichia coli BUG6, a temperature-sensitive cell division mutant, have shown that at the restrictive temperature (42 degrees C) the loss of cell division potential (filamentation) was accompanied by an unusual increase in intracellular cyclic GMP (cGMP). At the permissive temperature (30 degrees C), cell division proceeded normally, and cGMP did not accumulate. Increasing the osmotic strength of the medium with NaCl suppressed filamentation in BUG6 at 42 degrees C and also suppressed the temperature-sensitive accumulation of cGMP. The addition of nalidixic acid to BUG6 at 30 degrees C induced filamentation but failed to cause cGMP accumulation. A similar accumulation of cGMP has not been observed in other E. coli strains.
mesh_terms: D002455:Cell Division / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D007700:Kinetics; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
chemical_list: D009268:Nalidixic Acid; D012965:Sodium Chloride; D006152:Cyclic GMP