Structural requirements for the function of a yeast chromosomal replicator.
A sequence closely linked to the Saccharomyces cerevisiae HO gene confers autonomous replication in yeast. I have subjected this putative replication origin to deletion and point mutagenesis in order to identify structural features that are important requirements for autonomous replication in vivo. This analysis identifies a 14 bp core region, which is crucial for function and shows partial sequence conservation between a number of autonomously replicating sequences. Point mutations within the core region can abolish autonomous replication. The core region is flanked on one side by a sequence of about 20 bp, which is important for efficient autonomous replication. Deletion of this flanking sequence reduces, but does not necessarily eliminate, autonomous replication.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D002875:Chromosomes / Q000502:physiology*; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D005822:Genetic Vectors; D009154:Mutation; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D001616:beta-Galactosidase / Q000235:genetics
D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase