Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.
Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D013149:Spiroplasma / Q000235:genetics*; D014169:Transformation, Bacterial
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes