The possible proton translocating activity of the mitochondrial uncoupling protein of brown adipose tissue. Reconstitution studies in liposomes.
Loose coupling of thermogenic mitochondria of brown adipose tissue is related to a high proton (or hydroxyl) conductance of the inner membrane and to the presence of a unique 32 kDa uncoupling protein. Reconstitution experiments of the purified protein in liposomes are reported which suggest that this component could form proton channels in the membrane.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D006224:Cricetinae; D003565:Cytidine Diphosphate / Q000494:pharmacology; D005614:Freeze Fracturing; D006153:Guanosine Diphosphate / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D008081:Liposomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D011522:Protons
D002352:Carrier Proteins; D008081:Liposomes; D011522:Protons; C030559:uncoupler-binding protein, Bos taurus; D006153:Guanosine Diphosphate; D006165:Guanylyl Imidodiphosphate; D000249:Adenosine Monophosphate; D003565:Cytidine Diphosphate