Effect of ellagic and caffeic acids on covalent binding of benzo[a]pyrene to epidermal DNA of mouse skin in organ culture.
The covalent binding of the anti-diol epoxide of benzo[a]pyrene to cellular DNA of mouse skin in organ culture is affected by the presence of ellagic acid in the culture medium. At 10(-4) M, BaPDE /DNA formation is 40% less than that observed when no ellagic acid is present. Caffeic acid, a similar plant phenolic compound, demonstrates no inhibitory effect on BaPDE /formation. The plant phenolic acids do not drastically interfere with the metabolism of benzo[a]pyrene as shown by the BaP-metabolite profiles of the skin or of the culture medium.
D000818:Animals; D001564:Benzo(a)pyrene; D001578:Benzopyrans / Q000494:pharmacology*; D001580:Benzopyrenes / Q000378:metabolism*; D002109:Caffeic Acids / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism*; D002934:Cinnamates / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004610:Ellagic Acid / Q000494:pharmacology*; D004817:Epidermis / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009924:Organ Culture Techniques; D012867:Skin / Q000378:metabolism*
D001578:Benzopyrans; D001580:Benzopyrenes; D002109:Caffeic Acids; D002273:Carcinogens; D002934:Cinnamates; D004610:Ellagic Acid; D001564:Benzo(a)pyrene; C032174:7,8,9,10-tetrahydroxytetrahydrobenzo(a)pyrene; D004247:DNA