In vitro interaction between calcitonin and calmodulin.
The effects of salmon calcitonin and human calcitonin on the fluorescence spectra of a fluorescent conjugate of calmodulin with dansyl-chloride and on the calmodulin mediated activation of phosphodiesterase have been studied. We showed that salmon calcitonin provoked calcium-dependent modifications of the spectra of dansyl-calmodulin and completely inhibited the calmodulin mediated activation of phosphodiesterase with a maximum effect at the dose of 5.8 X 10(-7)M and 10(-7) M respectively. No appreciable effect of human calcitonin was observed in either system. This interaction of salmon calcitonin with calmodulin is similar to the interaction of certain neuropeptides and antipsychotic drugs to calmodulin and may explain certain of the peptide's pharmacological effects.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D002116:Calcitonin / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D004789:Enzyme Activation; D006801:Humans; D012474:Salmon; D013050:Spectrometry, Fluorescence
D002147:Calmodulin; D003619:Dansyl Compounds; D002116:Calcitonin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; C029038:dansyl chloride; D002118:Calcium