Neoplastic transformation induced by adeno 12-SV40 hybrid virus in skin fibroblasts from humans genetically predisposed to cancer.
The DNA hybrid adeno 12-SV40 virus induced neoplastic transformation of human skin fibroblasts derived from individuals with adenomatosis of the colon and rectum (ACR), an inherited cancer syndrome. The line established from the transformed ACR fibroblasts became stable and was initially virus productive, but infectious hybrid virus gradually decreased over serial passages. Cultured skin fibroblasts from normal individuals infected with adeno 12-SV40 also underwent morphological transformation and were virus productive. Although these cells had a prolonged life as compared with uninfected controls, they eventually died. All of the transformed cells contained both adeno 12 and SV40 large tumor antigens, formed large cell aggregates, grew in liquid growth medium above an agar base, and formed colonies in soft agar. The altered ACR cells became heteroploid and produced tumors when transplanted subcutaneously into nude mice. The results suggest that ACR individuals have increased susceptibility to neoplastic transformation by oncogenic tumor viruses.
D000260:Adenoviruses, Human / Q000472:pathogenicity*; D000328:Adult; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002648:Child; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005736:Gardner Syndrome / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D051381:Rats; D013539:Simian virus 40 / Q000472:pathogenicity*; D012867:Skin / Q000473:pathology*