Forskolin activation of an identified peptide-sensitive motoneurone in Aplysia.
Activation of a physiological response by the adenylate cyclase activator, forskolin, has been suggested as a new criterion for testing the role of cyclic AMP. In Aplysia, motoneurone B16, which innervates muscle 15, is activated by the peptide egg-laying hormone (ELH). In high magnesium-low calcium medium, used to block synaptic activity, forskolin produced a similar response to ELH. Forskolin, at a concentration of 100 microM, consistently activated the ELH-sensitive neurone; vehicle produced no response while 30 microM forskolin usually produced lower levels of activity than 100 microM. The data are consistent with cyclic AMP mediation of the ELH response.
D000818:Animals; D001048:Aplysia; D005576:Colforsin; D000242:Cyclic AMP / Q000502:physiology; D004224:Diterpenes / Q000494:pharmacology*; D007447:Invertebrate Hormones / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology
D004224:Diterpenes; D007447:Invertebrate Hormones; C026880:egg-laying hormone, Mollusca; D005576:Colforsin; D000242:Cyclic AMP