Circulating antibodies to the feline leukemia virus and FOCMA in cats.
Presence of antibodies to the structural proteins of the feline leukemia virus and the feline oncornavirus-associated cell membrane antigen FOCMA was analyzed in sera of 120 cats from household environments. In our experiments 35% of the sera tested were positive with the viable cells of FL-74 feline lymphoma cell line. On the other hand, only 5% of the feline sera reacted with FeLV from FL-74 cells in the solid phase RIA. Selected positive sera were analyzed for the presence of antibodies to the viral structural proteins of FeLV and FOCMA in lysates of FL-74 cells by radioimmunoprecipitation. Absorption of the selected sera (reacting with viable FL-74 cells) with disrupted FeLV had a negligible effect on the precipitating activity of the 70000 protein.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D002460:Cell Line; D007938:Leukemia / Q000276:immunology / Q000662:veterinary*; D005256:Leukemia Virus, Feline / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins; C029224:feline oncornavirus-associated cell membrane antigen