Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations.
Kirsten (Ki)-ras cDNA clones were prepared from human lung and colon carcinoma cell lines expressing an activated c-Ki-ras2 gene. DNA sequence analysis and transfection studies indicate that different point mutations at the same codon can activate the gene; that most human c-Ki-ras2 mRNA uses sequences from a fourth coding exon distinct from that of its viral counterpart; and that at least one cell line is functionally homozygous for the activated gene.
D000483:Alleles; D002471:Cell Transformation, Neoplastic; D003110:Colonic Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007708:Kirsten murine sarcoma virus / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D009857:Oncogenes; D012326:RNA Splicing; D012334:RNA, Neoplasm / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D014764:Viral Proteins