Purification of VDAC (voltage-dependent anion-selective channel) from rat liver mitochondria.
The outer membrane of rat liver mitochondria contains a channel-forming protein known as VDAC (voltage-dependent anion-selective channel). This protein has been functionally purified by a combination of ion exchange chromatography, gel filtration and affinity chromatography on a Concanavalin A-containing column. An estimated 300-fold purification was achieved over the specific activity in mitochondrial membranes. When the purified protein is run on an SDS polyacrylamide gel, essentially only one band is present at a position consistent with a molecular weight of 32,000. The resulting protein is functional and behaves normally based on channel size, selectivity and voltage dependence.
D000818:Animals; D000838:Anions; D002458:Cell Fractionation / Q000379:methods; D007425:Intracellular Membranes / Q000648:ultrastructure; D007473:Ion Channels / Q000502:physiology / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000302:isolation & purification; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D000838:Anions; D007473:Ion Channels; D008565:Membrane Proteins