Cloning and mapping of the RAD50 gene of Saccharomyces cerevisiae.
The RAD50 gene was cloned as a 4.8 kb fragment in the 2 mu derived plasmid pFL1. The gene resides in a 3.9 kb segment that was subcloned into the plasmid YRp7. The cloned gene complements the deficiency caused by the rad50-1 mutation with respect to gamma-rays, MMS resistance and UV-induced mitotic recombination. Restoration of the Rad+ phenotype occurs when the cloned gene is on a freely replicating multiple-copy plasmid or in the integrated form. Mapping of the cloned gene following integration of the 2 mu plasmid, and of the subclone in plasmid YRp7, showed it to be located on the left arm of chromosome XIV. Tetrad analysis of various crosses involving two different strains carrying rad50-1 showed the mutation to map next to pet2 on chromosome XIV, and not on the right arm of chromosome IV, as previously published.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005720:Gamma Rays; D005800:Genes, Fungal; D005838:Genotype; D008938:Mitosis; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects
D004262:DNA Restriction Enzymes