Molecular biological characterization of a highly leukaemogenic virus isolated from the mouse. III. Identity with mouse mammary tumour virus.
A highly leukaemogenic virus isolate (DMBA-LV) endogenous to the CFW/D mouse has been found to contain two viral genomes. One was closely related to the type B milk-borne mouse mammary tumour virus (MMTV) and present in tenfold excess over a type C viral genome which was only partially related to xenotropic and polytropic isolates from the CFW/D mouse as well as to the ecotropic Moloney murine leukaemia virus isolate. The thymic lymphoma cell line that produced DMBA-LV expressed high levels of MMTV viral RNA (35S and the 24S envelope mRNA). Both the virus and the virus-producing cell line expressed multiple species of type C viral RNA. Similar species of type C viral RNA were also associated with non-infectious, non-leukaemogenic viral particles present in both normal lymphoid cells and in a MMTV-free thymic lymphoma cell line established from a second chemical carcinogen-induced tumour.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D008223:Lymphoma / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D013953:Thymus Neoplasms / Q000382:microbiology; D014776:Virus Cultivation
D012367:RNA, Viral