Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.
DNA gyrase is the bacterial enzyme responsible for converting circular DNA to a negatively supercoiled form. We show that the synthesis of DNA gyrase is itself controlled by DNA supercoiling; synthesis is highest when the DNA template is relaxed. The rates of synthesis in vivo of both the A and B subunits of DNA gyrase are increased up to 10-fold by treatments that block DNA gyrase activity and decrease the supercoiling of intracellular DNA. Similarly, efficient synthesis of both gyrase subunits in a cell-free S-30 extract depends on keeping the closed circular DNA template in a relaxed conformation. The results suggest that DNA supercoiling in E. coli is controlled by a homeostatic mechanism. Synthesis of the RecA protein and several other proteins is also increased by treatments that relax intracellular DNA.
D001426:Bacterial Proteins / Q000096:biosynthesis; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006706:Homeostasis; D011938:Rec A Recombinases
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D011938:Rec A Recombinases; D004250:DNA Topoisomerases, Type II