title: Improvement of respiratory syncytial virus replication in actively growing HEp-2 cells.
abstract: The growth of respiratory syncytial (RS) and parainfluenza type III (PI3) viruses has been studied in actively growing versus relatively stationary HEp-2 cells. There was no effect on PI3 virus growth. RS virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. This stimulation was manifested by a greater yield of virus per cell, more virus-specific mRNA produced per cell, and a 50% decrease in the time before cytopathic changes appeared.
mesh_terms: D002452:Cell Count; D002455:Cell Division; D046508:Culture Techniques / Q000379:methods; D003588:Cytopathogenic Effect, Viral; D008828:Microbiological Techniques; D010224:Parainfluenza Virus 3, Human / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral