[Detection of an endogenous inhibitor of benzodiazepine receptor binding].
An original one-stage method for isolating from brain tissue of a water-soluble fraction containing an inhibitor of benzodiazepine specific binding is suggested and the properties of the inhibitor are described. The inhibitor is a thermostable compound with a molecular mass of 2000-10000 dalton. It inhibits diazepam binding depending on the concentration. The IC50 for diazepam binding is approximately ten-fold less than the inhibitor concentration in the brain tissue. The inhibitor is distributed heterogeneously in the rat brain (the maximal content in the cerebellum and cortex) and bovine brain (the maximal content in the thalamus and caudate nucleus).
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D003975:Diazepam / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011963:Receptors, GABA-A; D014020:Tissue Extracts / Q000494:pharmacology*
D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D014020:Tissue Extracts; D003975:Diazepam
Vyiavlenie éndogennogo ingibitora retseptornogo sviazyvaniia benzodiazepinov.