title: Properties of a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli.
abstract: A fragment of the cloned gene for the human myeloma ND epsilon chain, coding for the second, third, and fourth domains of the immunoglobulin, has been coupled to the tryptophan control region of an expression plasmid and subcloned in Escherichia coli. Induction of gene expression results in the synthesis of the expected, antigenically active polypeptide of Mr 40,000, which constitutes 18% of total bacterial protein and yields 55 mg/liter of culture. The immunoglobulin, which is aggregated and packed into large inclusion bodies within the bacterial cell, can be dissolved by denaturing solvents and purified by affinity chromatography using anti-IgE Sepharose. Reduced monomeric chains assemble spontaneously into dimers. On assay to measure the inhibition of binding of 125I-labeled human E myeloma protein to Fc epsilon receptors on cultured human basophils, the cloned gene product exhibited 20% of the activity of the native protein.
mesh_terms: D001491:Basophils / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics* / Q000276:immunology; D008854:Microscopy, Electron; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000235:genetics; D010957:Plasmids; D011961:Receptors, Fc / Q000276:immunology
chemical_list: D007143:Immunoglobulin Heavy Chains; D007139:Immunoglobulin epsilon-Chains; D009194:Myeloma Proteins; D011961:Receptors, Fc; D007073:Immunoglobulin E