title: Characterization of the synthesis and release of prolactin by an enriched fraction of human decidual cells.
abstract: An enriched fraction of human decidual cells that synthesizes and releases human PRL (hPRL) was obtained by isopycnic centrifugation of collagenase- and hyaluronidase-dispersed cells through Percoll. The cells that synthesized and released hPRL banded at a density of 1.017-1.045 g/ml, an area of the gradient comprising only a small percentage of the total decidual DNA. The enriched cells formed distinct colonies in culture and contained hPRL, as evidenced by indirect immunofluorescent staining with anti-PRL serum. Plated at a density of 5.0 x 10(5) cells/well, the cells produced hPRL at a mean rate of 8.1 +/- 1.1 ng/microgram DNA . 24 h (mean +/- SD) for 8 days. Like decidual explants, the enriched cells responded to phospholipase A2 (0.1 U/ml) with a 54% decrease in hPRL release and to placental conditioned medium (0.5 mg protein/ml medium) with a 62% increase. Insulin (8.3 x 10(-7) M), progesterone (10(-5) - 10(-12) M), and estradiol (10(-5) - 10(-12) M) did not affect hPRL release over 6 days. These results indicate that enriched PRL-releasing cells, obtained by the isopycnic centrifugation of collagenase- and hyaluronidase-dispersed cells, are a useful model for the study of the synthesis and release of PRL.
mesh_terms: D002469:Cell Separation; D002478:Cells, Cultured; D002500:Centrifugation, Isopycnic; D004247:DNA / Q000378:metabolism; D003656:Decidua / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase; D011247:Pregnancy; D011388:Prolactin / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D011506:Proteins / Q000378:metabolism
chemical_list: D011458:Prostaglandins E; D011506:Proteins; D011388:Prolactin; D004247:DNA; D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase