2-5A synthetase activity does not increase in interferon-resistant Friend leukemia cell variants treated with alpha/beta interferon despite the presence of high-affinity interferon receptor sites.
The presence of interferon (IFN) receptors on mouse Friend leukemia cells (FLC) has been investigated in binding experiments with highly purified 125I-labeled mouse alpha/beta IFN. Both IFN-resistant clones and wild-type IFN-sensitive FLC showed a specific saturable binding site for mouse IFN with a similar affinity constant. In contrast to IFN-sensitive FLC, IFN-resistant FLC variants were not inducible by IFN for double-stranded RNA-dependent 2-5A synthetase activity.
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D000818:Animals; D002999:Clone Cells / Q000378:metabolism; D005622:Friend murine leukemia virus; D007370:Interferon Type I / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017471:Receptors, Interferon
D007370:Interferon Type I; D011956:Receptors, Cell Surface; D017471:Receptors, Interferon; D015088:2',5'-Oligoadenylate Synthetase