Pseudorabies virus, porcine parvovirus, and porcine enterovirus interactions with the zona pellucida of the porcine embryo.
Porcine embryos (n = 93) were incubated on cell monolayers that had been previously inoculated with pseudorabies virus, porcine parvovirus (PPV), or each of 2 porcine enteroviruses. After 2, 24, or 48 hours of incubation, the embryos were fixed in glutaraldehyde and examined by electron microscopic procedures. It was found that pseudorabies virus adsorbed to the zona pellucida (ZP) and entered sperm tracks in the ZP. The PPV and both enteroviruses entered pores in the ZP and were associated with sperm that were at or near the outer surface of the ZP. In addition, PPV was seen enmeshed in cellular debris on the outer surface of the ZP. Evidence of a productive viral infection of the blastomeres of the embryos was not found.
D000818:Animals; D004770:Enterovirus / Q000254:growth & development*; D004771:Enteroviruses, Porcine / Q000254:growth & development*; D005260:Female; D011558:Herpesvirus 1, Suid / Q000254:growth & development*; D008297:Male; D010063:Ovum / Q000382:microbiology*; D010321:Parvoviridae / Q000254:growth & development*; D011247:Pregnancy; D013094:Spermatozoa / Q000382:microbiology; D013552:Swine / Q000196:embryology* / Q000382:microbiology; D014777:Virus Diseases / Q000635:transmission; D015044:Zona Pellucida / Q000382:microbiology*