Effects of treadmill running on the properties of guinea-pig myosin.
The effects of running training on the structure and function of ventricular myosin of guinea-pigs were studied. No differences in body or heart weights could be detected but the heart-to-body weight relation increased significantly (P less than 0.05) in the trained group. Ca2+ activated and K+ activated ventricular myosin ATPase activities as well as the electrophoretic appearances (SDS-PAGE, pyrophosphate-PAGE) did not change after training. Guinea-pig ventricular myosin in nondissociating conditions showed one band migrating close to rat-V3 isomyosin. The myosins of the trained and untrained animals also showed no immunological difference as determined by the competitive ELISA-test: they both shared antigenic determinants common to rat-V1 isomyosin.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism*; D005082:Physical Exertion; D011188:Potassium / Q000378:metabolism
D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases; D009218:Myosins; D011188:Potassium; D002118:Calcium