Glutaraldehyde stabilisation of antibody-linked erythrocytes for use in reverse passive and related haemagglutination assays.
A simple method for stabilising antibody-linked red blood cells by the addition of low concentrations of glutaraldehyde is described. Fresh and stabilised reagent-linked cells were shown to compare favourably in reverse passive haemagglutination for the measurement of human immunoglobulin isotypes, G, A and M and for the detection of respiratory syncytial and herpes simplex viruses. Stabilised cells were also used to detect antibodies to bacteria and to a soluble antigen adsorbed to a solid phase by mixed haemagglutination reactions.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002006:Brucellosis / Q000175:diagnosis; D002417:Cattle; D004355:Drug Stability; D004912:Erythrocytes / Q000276:immunology*; D005976:Glutaral; D006386:Hemagglutination Tests / Q000379:methods*; D012756:Sheep; D018139:Simplexvirus / Q000276:immunology* / Q000302:isolation & purification
D000906:Antibodies; D000911:Antibodies, Monoclonal; D005976:Glutaral