title: Cell receptors for the mammalian reovirus. I. Syngeneic monoclonal anti-idiotypic antibody identifies a cell surface receptor for reovirus.
abstract: We have prepared and characterized a syngeneic monoclonal anti-idiotype directed against the idiotype (Id3) of the anti-reovirus type 3 hemagglutinin B cell hybridoma 9BG5. We have shown this monoclonal antibody to be anti-idiotypic in a series of inhibition studies with purified sigma 1 (hemagglutinin) protein and with absorption studies on the idiotypic R1.1 cell line. That the idiotype Id 3 defined by the monoclonal anti-idiotype is distinct from H-2 was demonstrated by its presence on an idiotype-expressing H-2 negative cell line (R1.E). Binding of the anti-idiotype is relatively tunicamycin resistant and pronase/trypsin sensitive, which suggests that the recognition site of the anti-idiotypic receptor is poorly glycosylated. The presence of idiotype-like receptors on reovirus type 3 susceptible neurons may have important implications for the presence of related gene families between neural and lymphoid tissue. The implications of recognition by neuronal cells of an anti-idiotypic monoclonal antibody directed against the reovirus 3 attachment receptor for the pathogenesis of viral-induced auto-immunity are discussed.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D005260:Female; D006183:H-2 Antigens / Q000032:analysis; D006389:Hemagglutinins, Viral / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000276:immunology; D012089:Mammalian orthoreovirus 3 / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000378:metabolism; D011991:Receptors, Virus / Q000032:analysis*; D012088:Reoviridae Infections / Q000276:immunology* / Q000382:microbiology; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism; D013945:Thymoma / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D006389:Hemagglutinins, Viral; D007130:Immunoglobulin Idiotypes; D011991:Receptors, Virus; D014415:Tunicamycin