title: Eosinophil-particle interactions: a model system for study of cellular adherence and activation.
abstract: In its role as an effector capable of killing large multicellular parasites, the eosinophil must be especially adapted for dealing with noningestible surfaces. A model system of Sepharose beads coated with serum protein or concanavalin A (Con A) has been used to study interactions between guinea pig peritoneal exudate eosinophils and noningestible particles. A small percentage of eosinophils were adherent to serum treated Sepharose; however, many cells were adherent to Con A-Sepharose. Adherence to Con A-Sepharose was decreased by pretreatment with 1 mM alpha-methylmannoside (alpha-MM). As compared to resting eosinophils, incubation of eosinophils with serum-treated Sepharose led to activation of oxidative metabolism as indicated by an eight-fold increase in superoxide anion production and an approximately threefold increase in quantitative leukocyte iodination. Eosinophils which were adherent to Con A beads could not be activated by either phorbol myristate acetate (PMA) or preopsonized zymosan. However, if adherence was reduced by preincubation with alpha-MM, PMA was able to activate the eosinophils. Neither soluble Con A nor Sepharose beads interfered with the assay of superoxide anion. These studies demonstrate the utility of Sepharose beads for studying interactions between eosinophils and noningestible particles.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D006168:Guinea Pigs; D007700:Kinetics; D008766:Methylmannosides / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D012685:Sepharose / Q000031:analogs & derivatives / Q000494:pharmacology; D013481:Superoxides / Q000097:blood; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D008766:Methylmannosides; C028326:concanavalin A-sepharose; D013481:Superoxides; C008466:methylmannoside; D012685:Sepharose; D011112:Polymyxin B; D013755:Tetradecanoylphorbol Acetate