[A comparison of serological methods and their use in the diagnosis of enzootic bovine leukemia].
ELISA method with polystyrene micro-plates and rods was compared with the neutralization test in plaque modification (SN) and with the immunodiffusion method (ID). Micro-plates are the best for the adsorption of the virus, mainly in quantitative studies. In screening examinations it is also the diagnostic rods that give results comparable with the SN method. In the modified ELISA method, dubious results should also be regarded as diagnostically important. In mass examination, ELISA test can be considered as an alternative to the neutralization test. As demonstrated, the titres of antibodies from 80 to 100 determined by ELISA method in micro-plates or by the SN test represent the marginal titres of the positivity of ID test. The ELISA method is suitable mainly in the final stages of the sanitation of herds since infected animals are revealed with a high sensitivity and in time.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D009500:Neutralization Tests
Porovnání nĕkterých sérologických metod a jejich vyuzití v diagnostice enzootické bovinní leukémie.