title: Products derived from 5,8,11-eicosatrienoic acid by the 5-lipoxygenase-leukotriene pathway.
abstract: Analysis of products derived from 5,8,11-eicosatrienoic acid via the 5-lipoxygenase-leukotriene pathway showed that this fatty acid is readily converted to leukotriene (LT)A3. When 10,000 X g supernatant from rat basophilic leukemia cell homogenates was incubated with 30 microM fatty acid, 5,8,11-eicosatrienoic acid produced 6.2 +/- 1.1 nmol of LTA3 and arachidonic acid 15.5 +/- 1.9 nmol of LTA4 (n = 4). However, only insignificant amounts of LTB3 were formed (0.15 +/- 0.04 nmol of LTB3 and 4.2 +/- 0.4 nmol of LTB4, n = 4). These data indicate that the LTA-hydrolase requires not only the three double bonds of the triene but also the double bond at C-14 to efficiently convert LTA to LTB. These findings have significant implications for essential fatty acid deficiency.
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism* / Q000031:analogs & derivatives; D000818:Animals; D001091:Arachidonate Lipoxygenases; D001095:Arachidonic Acids / Q000096:biosynthesis* / Q000302:isolation & purification; D001491:Basophils / Q000201:enzymology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007536:Isomerism; D007942:Leukemia, Experimental / Q000201:enzymology*; D017572:Leukotriene A4; D008084:Lipoxygenase / Q000378:metabolism*; D013058:Mass Spectrometry; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D017572:Leukotriene A4; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase; D015126:8,11,14-Eicosatrienoic Acid; C010944:mead acid