title: Human colon cells: culture and in vitro transformation.
abstract: Normal human colon mucosal epithelial cells were cultured in vitro and treated with the oncogenic simian DNA virus (SV40) and the chemical carcinogen azoxymethane. Both SV40 and azoxymethane altered a number of phenotypic characteristics of the normal human colon cells, including their morphology, culture longevity, growth in soft agar, substrate adherence, and peanut agglutinin binding. The SV40 transformants synthesized intranuclear T antigen. These data indicate that normal human colon mucosal cells were transformed toward the malignant phenotype.
mesh_terms: D000957:Antigens, Viral, Tumor / Q000378:metabolism; D001397:Azoxymethane / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000503:physiopathology*; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology* / Q000187:drug effects; D003110:Colonic Neoplasms / Q000503:physiopathology; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D013539:Simian virus 40 / Q000378:metabolism
chemical_list: D000957:Antigens, Viral, Tumor; D037102:Lectins; D001397:Azoxymethane