Inhibition of galactose-induced alterations in ocular lens with sorbinil.
Lens ultrastructure and Na- K-ATPase activity in the lenses of rats fed galactose and a galactose + sorbinil diet (aldose reductase inhibitor) were studied. Lenses of rats on the galactose diet exhibited development of peripheral opacity within 3-4 days. This opacity progressed with the continuation of the galactose feeding, and by 20 days mature cataracts were observed in these animals. The formation of vacuoles, cysts, membrane disruption in the epithelium and fibers, and swelling of fibers accompanied the development of opacity. With the progression of opacity there was a considerable drop in lens Na- K-ATPase activity in the galactose-fed animals. However, the lenses of rats that were treated with sorbinil did not show any of the alterations in the ultrastructure of the epithelium and fibers that accompany galactose cataractogenesis. The level of Na-K-ATPase activity in the sorbinil-treated animals was similar to that found in lenses from the laboratory chow-fed group of rats. These observations further substantiate the role of aldose reductase in sugar-cataract development.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D005690:Galactose / Q000037:antagonists & inhibitors*; D007093:Imidazoles / Q000494:pharmacology*; D048289:Imidazolidines; D007908:Lens, Crystalline / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D007093:Imidazoles; D048289:Imidazolidines; D000449:Aldehyde Reductase; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; C026411:sorbinil; D005690:Galactose