Target cell lysis mediated by concanavalin A-triggered human neutrophils.
Human neutrophils, triggered by Concanavalin A, were cytotoxic against chicken red blood cell targets as determined by the 51Cr release method. The cytolysis increased with the effector: target ratio, reaching optimal levels when 2-4 neutrophils were available for each chicken red blood cell. The target cell lysis required an optimal release of highly reactive oxygen by-products by neutrophils, since neutrophils from a patient with chronic granulomatous disease failed to exhibit any cytolytic activity. Superoxide dismutase, catalase and inhibitors of heme-containing peroxidases (azide and cyanide) significantly inhibited the neutrophil-mediated cytotoxicity. Together these results indicate that superoxide anion and the myeloperoxidase-hydrogen peroxide system are simultaneously involved in the target cell injury by Concanavalin A-triggered neutrophils.
D000328:Adult; D002648:Child; D002860:Chromium Radioisotopes; D003208:Concanavalin A / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006105:Granulomatous Disease, Chronic / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D002860:Chromium Radioisotopes; D003208:Concanavalin A; D013481:Superoxides; D006861:Hydrogen Peroxide; D009195:Peroxidase