Detection of antibodies against reticuloendotheliosis viruses by an enzyme-linked immunosorbent assay.
A microplate indirect enzyme-linked immunosorbent assay (ELISA) for antibodies against reticuloendotheliosis virus (REV) was consistently more sensitive than indirect immunofluorescent-antibody tests. Limits of antibody detection were comparable to those obtained in virus neutralizations. Detection of REV-infected chickens long after infection and after immunofluorescent antibody has waned makes ELISA especially suitable for screening chicken flocks.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000175:diagnosis*; D001356:Reticuloendotheliosis virus / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000662:veterinary*
D000914:Antibodies, Viral