title: Modification of sheep pineal beta-adrenoceptors by some gonadal steroids but not by melatonin.
abstract: The effects of active immunization against a range of gonadal steroids on pineal beta-adrenoceptors were studied in cycling Merino ewes. In selected cases, the effects of exogenous steroids were also investigated. Immunization against progesterone had no detectable effect on pineal beta-adrenoceptor density or ligand binding affinity. Immunization against estrone or 17 beta-estradiol significantly increased binding affinity and decreased beta-receptor density. Testosterone immunization in ewes caused similar effects but to a lesser degree. In contrast, immunization against androstenedione resulted in a decreased affinity and an increase in receptor density in ewes. In subsequent experiments, some parameters of pineal function in intact cycling and ovariectomized ewes were compared; a significant decrease in beta-receptor density and an increase in binding affinity were noted in the ovariectomized animals. Androstenedione-releasing implants decreased beta-receptor density and increased beta-receptor affinity in pineals from intact ewes, but these implants had no effect on pineal parameters in ovariectomized ewes. Immunization of ewes against 17 beta-estradiol had no significant effects on basal or isoprenaline-stimulated N-acetyltransferase or adenyl cyclase activities. Immunization of ewes against melatonin, or in vitro incubation of pineal glands with the hormone had no effects on the pineal parameters studied. It is concluded that estrogens affect the pinealocyte beta-receptors in ewes, while specific androgens may act indirectly on these receptors to modify their interaction with estrogens.
mesh_terms: D000735:Androstenedione / Q000502:physiology; D000818:Animals; D002369:Castration; D005260:Female; D012739:Gonadal Steroid Hormones / Q000276:immunology / Q000502:physiology*; D007114:Immunization; D008027:Light; D008550:Melatonin / Q000276:immunology / Q000502:physiology*; D010870:Pineal Gland / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012756:Sheep; D013997:Time Factors
chemical_list: D012739:Gonadal Steroid Hormones; D011943:Receptors, Adrenergic, beta; D000735:Androstenedione; D008550:Melatonin