Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells.
ATP (approx. 1-300 microM) induces dose-dependent prostacyclin secretion from perfused columns of microcarrier beads with cultured endothelial monolayers attached. The response is transient, shows little tachyphylaxis and can reach approx. 100 times control values. 2-Methylthio-ATP is more potent, ADP slightly less potent, AMP much less potent and adenosine is ineffective. These results are consistent with the presence of a purinoceptor on endothelium linked to the prostacyclin synthetic pathway.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects*; D011983:Receptors, Purinergic; D013268:Stimulation, Chemical; D013552:Swine
D000227:Adenine Nucleotides; D011453:Prostaglandins; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D011464:Epoprostenol