Sodium dodecyl sulfate-mediated transfer of electrophoretically separated DNA-binding proteins.
A modified procedure for the transfer of electrophoretically-separated proteins from sodium dodecyl sulfate (SDS)-polyacrylamide gels onto nitrocellulose filters has been developed. During the diffusion mediated transfer, the SDS-protein complexes were maintained and SDS was added to the buffer. This increases the number of polypeptide species bound to the filter thereby giving an accurate replica of the original gel pattern. The immobilization in the gel of certain polypeptides characterized as DNA-binding proteins, which is observed when SDS is eliminated prior to blotting is avoided. The molecules blotted in the presence of SDS remain immunoreactive and able to bind DNA.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002021:Buffers; D002460:Cell Line; D003101:Collodion; D006224:Cricetinae; D004265:DNA Helicases / Q000302:isolation & purification*; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D010053:Ovary; D010455:Peptides / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000302:isolation & purification
D000956:Antigens, Viral; D002021:Buffers; D004268:DNA-Binding Proteins; D010455:Peptides; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D003101:Collodion; D004265:DNA Helicases