Specificity of thyroglobulin interactions with thyroid cells and membranes.
Homologous species specificity is demonstrated with bovine and human thyroglobulin in which the two terminal sugars of the B carbohydrate chain, sialic acid and galactose have been removed by enzymatic hydrolysis. The species specificity is demonstrated by measuring the ability of the deglycosylated thyroglobulin derivatives to inhibit thyrotropin-induced increases in cAMP in human, rat and bovine thyroid cells in culture. Thus human-human or bovine-bovine interactions have higher activity coefficients by at least an order of magnitude than their heterologous counterparts. The homologous interactions are confirmed in binding studies and shown to be associated with negligible degradation of the bound ligand over a 24 hour period.
D000818:Animals; D001212:Asialoglycoproteins; D002417:Cattle; D002478:Cells, Cultured; D004705:Endocytosis; D006023:Glycoproteins / Q000494:pharmacology / Q000502:physiology; D006801:Humans; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects; D011989:Receptors, Thyrotropin; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013954:Thyroglobulin / Q000378:metabolism*; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D013972:Thyrotropin / Q000037:antagonists & inhibitors
D001212:Asialoglycoproteins; D006023:Glycoproteins; D011956:Receptors, Cell Surface; D011989:Receptors, Thyrotropin; D013972:Thyrotropin; D013954:Thyroglobulin