[Importance of immunoelectrophoresis in agar and disc electrophoresis in polyacrylamide gel for characterizing different strains of Bordetella pertussis].
The antigenic composition of typical and atypical B. pertussis strains obtained in the foci of pertussis infection, as well as experimentally obtained antibiotic-resistant B. pertussis strains, has been studied by the methods of immunoelectrophoresis in agar and electrophoresis in polyacrylamide gel (PAAG). Immunoelectrophoresis in agar has been found capable of differentiating B. pertussis culture from a group of unidentified morphologically similar Gram-negative bacilli by their antigenic composition and thus suitable for use as an additional criterion in the identification of atypical B. pertussis strains. PAAG electrophoresis has permitted finding differences in the set of protein antigens in the control strain and in its clones obtained by multiple subculturing in media with antibiotics added.
D000362:Agar; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001886:Bordetella pertussis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007114:Immunization; D007122:Immunoelectrophoresis / Q000379:methods*; D011817:Rabbits
D000942:Antigens, Bacterial; D000362:Agar
Znachenie metoda immunoélektroforeza v agare i diskovogo élektroforeza v poliakrilamidnom gele dlia kharakteristiki razlichnykh shtammov Bordetella pertussis.