title: Intracellular pH, transepithelial pH gradients, and ADH-induced water channels.
abstract: Urinary bladders of frogs were exposed to a transepithelial proton and osmotic gradient (serosal pH 8.1, Tris or bicarbonate buffer; mucosal pH 5.8, unbuffered) while the alkalinization rate of the mucosal bath and the net water movement were simultaneously monitored. It was observed that 1) the mucosal alkalinization rate was dependent on serosal pH and buffer; 2) oxytocin increased the mucosal alkalinization rate only when serosal bicarbonate was employed, whereas the net water movement augmented both when serosal bicarbonate or Tris buffers were used; 3) amiloride did not modify the mucosal alkalinization rate either before or after oxytocin; 4) the increases in the mucosal alkalinization rate and in the net water movement induced by oxytocin (serosal bicarbonate) were negatively correlated. In other experiments intracellular pH (pHi) was estimated with the DMO distribution technique with the following results. 1) Oxytocin increased the pHi when either serosal bicarbonate or Tris buffers was used and even in the presence of a low mucosal pH (Tris buffer, pH 5.8). 2) Important cellular acidification was observed when CO2 was bubbled (to pH 5.8), whereas the hydrosmotic response to 8-bromo-cAMP was clearly inhibited. These results indicate that cellular alkalinization could play a pivotal role in action of ADH, show that ADH can modify the transepithelial pH equilibrium mechanism, and suggest that intracellular pH regulation and water permeability control can be linked regulatory processes.
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate; D000818:Animals; D001834:Body Water / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004848:Epithelium / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D009092:Mucous Membrane / Q000502:physiology; D010121:Oxytocin / Q000494:pharmacology*; D011893:Rana esculenta; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
chemical_list: D002245:Carbon Dioxide; D015124:8-Bromo Cyclic Adenosine Monophosphate; D010121:Oxytocin; D000242:Cyclic AMP