Stimulation of the Na/K pump in LK sheep erythrocytes by immunoglobulin fragments.
Alloimmune antiserum against the L antigen of red cells from sheep of the LK phenotype is known to stimulate by several fold active Na/K transport in LK cells. We have shown that monomeric fragments, Fab1, of anti-L, as well as dimeric fragments, F(ab1)2, stimulate transport to the same extent as intact anti-L Ig. Special care was taken to obtain pure fragments. Two earlier reports on the effect of immunoglobulin fragments were contradictory.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001693:Biological Transport, Active; D001789:Blood Group Antigens / Q000276:immunology*; D004912:Erythrocytes / Q000201:enzymology / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007518:Isoantibodies / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D012756:Sheep / Q000097:blood; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013268:Stimulation, Chemical
D001789:Blood Group Antigens; D007140:Immunoglobulin Fab Fragments; D007518:Isoantibodies; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium