Accumulation of herpes simplex virus type 1 glycoprotein D in adhesion areas of infected cells.
By indirect immunofluorescence microscopy (IIF) we have localized glycoprotein D (gD) of herpes simplex virus-infected cells to the vinculin-containing junctional areas and to the focal adhesion sites on the ventral surface of the cells. Double-staining IIF showed that gD and vinculin co-distributed in the infected cells and treatment of the infected cells 4 h post-infection with rabbit antibodies to gD prevented attachment of the cells to the growth substratum. Our results therefore support the hypothesis that gD plays a central role in the social behaviour of infected cells.
D001692:Biological Transport; D002448:Cell Adhesion; D002451:Cell Compartmentation; D002478:Cells, Cultured; D006023:Glycoproteins / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D018139:Simplexvirus / Q000378:metabolism*; D016596:Vinculin; D014764:Viral Proteins / Q000378:metabolism*
D006023:Glycoproteins; D009124:Muscle Proteins; D014764:Viral Proteins; D016596:Vinculin