Efficient DNA-mediated transfer of selectable genes and unselected sequences into differentiated and undifferentiated mouse melanoma clones.
We have found that three phenotypically dissimilar mouse B16 melanoma subclones are competent recipients for DNA-mediated gene transfer. Two of these approach and a third, amelanotic clone B78H1, surpasses mouse LTK cells in frequencies of transferent colony formation after treatment with either of two codominantly selectable plasmid vectors, pSV2gpt or pGCcos3neo. Melanoma transferents incorporate both selectable plasmid-homologous sequences and substantial amounts of unselected donor DNA into their cellular DNAs. In addition they retain the distinctive states of differentiation characteristic of the untreated clones. Frequencies of pGCcos3neo-mediated transfer of neo gene-encoded antibiotic resistance into B78H1 can reach 10(-2) in response to treatment with as little as 15 ng plasmid/ml coprecipitate/dish. B78H1 cells readily give rise to "secondary" transferents for the neo gene after treatment with DNA from a "primary" B78H1 neo transferent. This gene transfer system has potential applications for study of regulation of melanoma and neural crest differentiation and malignancy.
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D019868:Kanamycin Kinase; D008545:Melanoma / Q000235:genetics / Q000503:physiopathology*; D051379:Mice; D010430:Pentosyltransferases / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D010957:Plasmids; D013937:Thymidine Kinase / Q000235:genetics
D004273:DNA, Neoplasm; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D010770:Phosphotransferases; D013937:Thymidine Kinase; D019868:Kanamycin Kinase