title: Neurotrophic and neuronotrophic effects in the regenerating newt limb bud after electrical stimulation of brachiospinal nerves.
abstract: An earlier work demonstrated that electrical stimulation of newt brachiospinal nerves produces a 20% increase in protein synthesis in the regenerating limb bud at 6 h post-stimulation. The present study shows that if stimulation of nerve cell bodies is prevented by placing procaine between the cell bodies and the stimulating electrode, there is no increase in limb bud protein synthesis compared to the non-stimulated, contralateral control limb bud. Similarly, if colchicine is applied to the brachiospinal nerves at the site of and prior to stimulation, there is no increase in limb bud protein synthesis after stimulation. Colchicine applied to brachiospinal nerves in the absence of stimulation results in a reduction of limb bud protein synthesis that is of the same magnitude as the increase seen with stimulation. The results suggest that the neurotrophic increase in limb bud protein synthesis after stimulation is under the control of the cell body and that this control is mediated by changes in fast axonal transport. A neuronotrophic increase in axonal density in the stimulated side limb bud is seen at the same time as the increase in protein synthesis after stimulation.
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D004558:Electric Stimulation; D005552:Forelimb / Q000294:innervation*; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009673:Notophthalmus viridescens; D013116:Spinal Cord / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: D009419:Nerve Tissue Proteins