Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes.
(3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25 degrees C was rapid, saturable (Bmax = 14.8 +/- 3.9 fmol/mg protein) and of high affinity (Kd = 0.15 +/- 0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine not equal to nisoldipine not equal to nifedipine much greater than verapamil. Cromolyn, however, neither inhibited nor increased the binding.
D000818:Animals; D002121:Calcium Channel Blockers / Q000378:metabolism; D015220:Calcium Channels; D002417:Cattle; D004205:Cromolyn Sodium / Q000378:metabolism; D007700:Kinetics; D009130:Muscle, Smooth / Q000378:metabolism*; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000378:metabolism; D015737:Nisoldipine; D009568:Nitrendipine; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*; D014700:Verapamil / Q000378:metabolism
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D011978:Receptors, Nicotinic; D015737:Nisoldipine; D009568:Nitrendipine; D014700:Verapamil; D009543:Nifedipine; D004205:Cromolyn Sodium