Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus.
Viral DNA was extracted from Crandell feline kidney (CRFK) cells infected with Aleutian disease virus (ADV) and labeled with [ 3H ]thymidine. The sedimentation coefficient in alkaline sucrose gradients was 16S corresponding to a molecular weight of 1.5 X 10(6). The buoyant densities of DNA from infected and control cells were determined by isopyknic sedimentation in CsCl and NaI gradients. Two additional peaks of [ 3H ]DNA were found in infected cells, but not in control cell extracts. Fractionation of this DNA on hydroxylapatite indicated that the new peaks represented a single-stranded component, density 1.728 g/cm3, and a double-stranded component, presumed to be a viral replicative intermediate, density 1.718 g/cm3. The target antigen formation in CRFK cells was measured by gamma-irradiation of ADV and assayed for focus formation. The calculated size of ADV based on these measurements was 1.1 X 10(6). The H-1 parvovirus also was shown to have a size of 1.5 X 10(6) daltons for both antigen and plaque formation. The data indicated similarities existed between ADV and other autonomously replicating parvoviruses in most properties, except that less-than-unit length genome of ADV may be transcribed.
D000454:Aleutian Mink Disease Virus / Q000032:analysis* / Q000528:radiation effects; D000818:Animals; D002415:Cats; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005720:Gamma Rays; D007668:Kidney; D008970:Molecular Weight; D014713:Viruses, Unclassified / Q000032:analysis*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA