In vitro infection of human B lymphocytes with adult T-cell leukemia virus.
Experimental transmission of adult T-cell leukemia (ATL) virus (ATLV) into human B lymphocytes was attempted. Cocultivation of B-cell rich fraction of peripheral blood from a healthy adult with X-ray irradiated ATLV producer MT-2 cells resulted in the establishment of OKA(B) cell line co-infected with both Epstein-Barr virus (EBV) and ATLV. OKA(B) cells and its subclones contained: (1) B cell markers exclusively; (2) both EBV-specific antigen, EBNA and ATLV-specific antigen, ATLA detected by immunofluorescence test; (3) ATLV-specific polypeptides, p24 and p19; (4) ATLV-specific mRNA in ATLA-positive clones; (5) ATLV and EBV particles detectable by electron microscopy. These data clearly show that human B lymphocytes are susceptible to ATLV infection.
D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D002999:Clone Cells; D017977:Deltaretrovirus / Q000276:immunology / Q000472:pathogenicity / Q000648:ultrastructure; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000302:isolation & purification
D000956:Antigens, Viral; D012367:RNA, Viral