title: Protein synthesis in cells infected by murine hepatitis viruses JHM and A59: tryptic peptide analysis.
abstract: The structural and intracellular proteins of the murine hepatitis viruses MHV-JHM and MHV-A59 were studied by tryptic peptide mapping. The results demonstrated that the virions contained three distinct proteins: the two related chains of the E2 complex, the nucleocapsid protein and the heterogeneous E1 complex. Five distinct virus-specific proteins were synthesized by infected cells. Three of the five intracellular proteins contained tryptic-peptides with properties similar to the three structural proteins. Models describing the evolution of the proteins are proposed. Although the pathogenic properties of MHV-JHM and MHV-A59 differ greatly, the tryptic peptide maps of the corresponding proteins of these MHV strains were remarkably similar.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006517:Murine hepatitis virus / Q000378:metabolism*; D010455:Peptides / Q000032:analysis*; D013045:Species Specificity; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D015678:Viral Structural Proteins
chemical_list: D010455:Peptides; D014764:Viral Proteins; D015678:Viral Structural Proteins