[Possible participation of a free-radical lipid intermediate in the hydroxylation of polycyclic hydrocarbons].
The effects of pro-oxidants (Fe2+, CCl4) and antioxidants (alpha-tocopherol, propyl gallate) on hydroxylation of polycyclic hydrocarbon benz(a)pyrene and on the NADPH-cytochrome P-450-reductase reaction were studied. The role of allyl radicals formed in the fatty acid chains in discussed. The binding of O2 to free radicals, i. e. formation of peroxy radicals, is regarded only as a possible reaction of the radical utilization. It is assumed that other reactions involving lipid radicals, in particular, electron transfer from the flavoprotein to cytochrome P-480 may occur in microsomal membranes.
D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002273:Carcinogens / Q000378:metabolism; D004579:Electron Transport; D005609:Free Radicals; D006900:Hydroxylation; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011083:Polycyclic Compounds / Q000378:metabolism*; D011435:Propyl Gallate / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000494:pharmacology
D001580:Benzopyrenes; D002273:Carcinogens; D005609:Free Radicals; D008054:Lipid Peroxides; D009153:Mutagens; D011083:Polycyclic Compounds; D014810:Vitamin E; D001564:Benzo(a)pyrene; D011435:Propyl Gallate; D002251:Carbon Tetrachloride; D007501:Iron; D009251:NADPH-Ferrihemoprotein Reductase; D010100:Oxygen
Vozmozhnoe uchastie svobodnoradikal'nogo lipidnogo intermediata v gidroksilirovanii politsiklicheskikh uglevodorodov.