Two Epstein-Barr viral nuclear neoantigens distinguished by gene transfer, serology, and chromosome binding.
We recently identified, by means of cotransformation of LTK- cells, a region of the Epstein-Barr virus (EBV) genome (the BamHI K fragment) that encodes or induces an EBV nuclear neoantigen (EBNA) serologically related to the EBNA found in lymphoid cells carrying the entire EBV genome. We now find that a second EBV DNA fragment, BamHI M, is also able to give rise to cotransformed LTK- cells with stable expression of a nuclear antigen. The BamHI K and M fragments have no apparent DNA homology. Many human sera that are reactive to EBNA in Raji cells detect both antigens; however, certain anti-EBNA-positive human sera are discordant and react only with the BamHI M or only with the BamHI K nuclear antigen. Every Raji cell appears to express both "M" and "K" antigens; D98 Raji cells, a somatic cell hybrid, express only "K" antigen. The K antigen is found on metaphase chromosomes of LTK cells and Raji cells. The M-induced antigen is not located on chromosomes when the cells are in metaphase but is present as granules within the nucleus.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D002051:Burkitt Lymphoma; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002875:Chromosomes / Q000276:immunology; D004262:DNA Restriction Enzymes; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007739:L Cells / Q000502:physiology; D051379:Mice; D009693:Nucleic Acid Hybridization
D000956:Antigens, Viral; D004262:DNA Restriction Enzymes