Cloning of the iron superoxide dismutase gene (sodB) in Escherichia coli K-12.
A clone overproducing iron superoxide dismutase has been isolated from an Escherichia coli cosmid bank. Subcloning located the gene responsible for iron superoxide dismutase overproduction on a 6.6-kilobase PstI restriction endonuclease fragment. Maxicell analysis, followed by immunological identification of iron superoxide dismutase protein, demonstrated that the structural gene, sodB, of iron superoxide dismutase has been cloned.
D001482:Base Composition; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D010957:Plasmids; D013482:Superoxide Dismutase / Q000235:genetics*
D013482:Superoxide Dismutase; D004262:DNA Restriction Enzymes