Monoclonal antibodies to rat brain alpha-adrenoceptors.
We have developed three hybridomas that produce monoclonal antibodies to rat cerebral alpha-adrenoceptors. Splenic lymphocytes from BALB/c mice immunized with unpurified digitonin-solubilized alpha-adrenoceptors were fused with the mouse myeloma line P3-x 63-Ag 8.653 to yield hybridoma cultures producing alpha-adrenoceptor monoclonal antibodies of the IgG class. However, these antibodies inhibited both alpha 1 and alpha 2 ligand binding suggesting that some molecular homology exists between alpha 1- and alpha 2-adrenoceptors in the rat brain.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002540:Cerebral Cortex / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011942:Receptors, Adrenergic, alpha