[Inhibiting effect of colcemid on the development of cytopathic changes due to the Sindbis virus].
The model of Chinese hamster (Cricetulus griseus) cell line infected with Sindbus virus was used to show that the presence in the maintenance medium of 0.01-0.1 micrograms/ml of colcemide inhibited the development of cytopathic changes, accumulation of viral antigens, and virus-specific complement-dependent cytolysis without affecting infectious virus production. The addition to the medium of 0.5% immune serum to Sindbis virus resulted not only in a similar inhibition but in a decrease of infectious virus titres. The latter effect was observed upon the addition of both colcemide and antiserum. Possible mechanisms of inhibition are discussed.
D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D003588:Cytopathogenic Effect, Viral; D003602:Cytotoxicity, Immunologic; D003703:Demecolcine / Q000494:pharmacology*; D003864:Depression, Chemical; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D008938:Mitosis; D011817:Rabbits; D012845:Sindbis Virus / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity; D014036:Togaviridae Infections / Q000276:immunology / Q000473:pathology*; D014776:Virus Cultivation
D007106:Immune Sera; D003703:Demecolcine
Ingibiruiushchee vliianie koltsemida na razvitie tsitopaticheskikh izmeneniĭ, vyzvavaemykh virusom Sindbis.