[Structural and enzymatic disorganization of biological membranes in rat liver cells in thermal burns].
Permeability of hepatocyte cell membrane was studied from the release into blood of hepatospecific enzymes and from 5'-nucleotidase activity in plasma membranes. A study was also made of membrane permeability of mitochondria, lysosomes and microsomes in liver cells of burnt rats from the level of non-sedimented activity and activity of malate dehydrogenase, succinate dehydrogenase, cathepsin D and glucose-6-phosphatase in appropriate organelles. Permeability of cell and lysosomal membranes was demonstrated to be disordered within the first hours after burn. One day after burn generalized disturbance of membrane permeability in the cell was observed, followed by the release into cytosol of organelles template enzymes and a decrease in the activity of membrane-bound enzymes in these organelles. The alterations persisted during 7 days of observation.
D015720:5'-Nucleotidase; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002056:Burns / Q000473:pathology*; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002463:Cell Membrane Permeability; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006638:Histidine Ammonia-Lyase / Q000097:blood; D008099:Liver / Q000473:pathology*; D008247:Lysosomes / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism; D009954:Ornithine Carbamoyltransferase / Q000097:blood; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D009954:Ornithine Carbamoyltransferase; D000410:Alanine Transaminase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D005952:Glucose-6-Phosphatase; D002403:Cathepsins; D002402:Cathepsin D; D006638:Histidine Ammonia-Lyase
K voprosu o strukturnoĭ i fermentnoĭ dezorganizatsii biologicheskikh membran v kletkakh pecheni krys pri termicheskikh ozhogakh.