gamma 1-Melanotropin-like immunoreactivity in bovine and human adrenocorticotropin-producing tissues.
gamma 1 MSH-like immunoreactivity in bovine pituitary glands, hypothalami, human pituitary glands, ectopic ACTH-producing tumors, and human gastric antral mucosa was investigated using a newly developed RIA for gamma 1 MSH. The minimum detectable quantity of gamma 1 MSH was 3 pg/tube, and the antibody did not cross-react with gamma 2 MSH, gamma 3 MSH, alpha MSH, beta MSH, ACTH, beta-lipotropin, or beta-endorphin. Bovine anterior pituitary, intermediate-posterior pituitary, and hypothalamus contained more than two molecular weight forms of gamma 1 MSH-like immunoreactivities. On the other hand, gamma 1 MSH-like immunoreactivity was not detected in human pituitary glands or human gastric antral mucosa, but was detected in one of seven ectopic ACTH-producing tumors. These results suggest a limited processing of the ACTH-beta-lipotropin precursor to gamma 1 MSH in human tissues, although a rapid degradation of gamma 1 MSH could not be ruled out completely.
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D000818:Animals; D002277:Carcinoma / Q000032:analysis; D002417:Cattle; D002850:Chromatography, Gel; D005753:Gastric Mucosa / Q000032:analysis*; D006801:Humans; D007031:Hypothalamus / Q000032:analysis*; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis* / Q000276:immunology; D009369:Neoplasms / Q000032:analysis* / Q000378:metabolism; D010902:Pituitary Gland / Q000032:analysis*; D010908:Pituitary Hormones, Anterior / Q000032:analysis / Q000276:immunology; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors / Q000032:analysis / Q000276:immunology; D011863:Radioimmunoassay; D013964:Thyroid Neoplasms / Q000032:analysis
D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones