Human cytomegalovirus (strain AD169) contains sequences related to the avian retrovirus oncogene v-myc.
We have detected nucleotide sequences related to the transforming gene (v-myc) of avian myelocytomatosis virus MC-29 in the DNA genome of human cytomegalovirus (HCMV) strain AD169. Cloned DNA representing the entire 1.5-kilobase-pair oncogene v-myc and subfragments of this gene were hybridized to EcoRI-cleaved HCMV virion DNA and cloned subgenomic HCMV DNA fragments. Only a 0.5-kilobase-pair Pst I-Sal I subfragment representing the 5' end of the coding sequence of the v-myc oncogene hybridized to the HCMV DNA. We have localized these v-myc-related sequences to five regions in the long unique segment of the HCMV genome corresponding to EcoRI fragments C, I, P, R, and b and to regions within the EcoRI junction fragments F and H at or near the repeats bounding the short unique segment. There was no hybridization of these HCMV sequences to other retroviral oncogenes tested including v-src, v-myb, v-erb, v-ST-fes, v-fos, v-ras (Harvey), v-mos, and v-abl.
D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D013045:Species Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes