title: Solubilization of ligand-stabilized vasopressin receptors from plasma membranes of bovine kidney and rat liver.
abstract: The solubilization of vasopressin receptors from plasma membranes of bovine kidney and rat liver by different detergents was investigated. A prerequisite for the extraction of vasopressin receptors retaining binding affinity for their ligand was the stabilization of the receptors by the prior formation of the membrane-bound hormone-receptor complexes. The vasopressin-receptor complexes from both kidney and liver membranes were solubilized in a high yield with dodecyl-beta-D-maltoside and 3-laurylamido-N,N'-dimethylpropylaminoxide. Several other nonionic detergents including octyl-beta-D-glucopyranoside effectively extracted the hepatic vasopressin receptor. For the hormone-receptor complex solubilized from bovine kidney with dodecyl-beta-D-maltoside, a Stokes' radius of 5.8 nm was determined.
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D003902:Detergents; D007668:Kidney / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D051381:Rats; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D017483:Receptors, Vasopressin; D012995:Solubility; D014667:Vasopressins
chemical_list: D003902:Detergents; D011956:Receptors, Cell Surface; D017483:Receptors, Vasopressin; D014667:Vasopressins; D001127:Arginine Vasopressin