The differential expression of the cellular src-gene product pp60src and its phosphokinase activity in normal chicken cells and tissues.
Chick embryos of different ages and adult chickens were examined for the expression of pp60c -src, the normal cellular homolog of the transforming protein of Rous sarcoma virus. In any brain extract, the pp60c -src kinase activity was always high, whereas muscle extracts of embryos show an age-dependent decrease in kinase activity. Adult animals show either no or barely measurable activity in muscle tissue. In contrast, liver cell extracts of embryos show an age-dependent increase in pp60c -src kinase activity, with adult chickens displaying the highest activity, very similar to that found in brain extracts. This demonstrates that increased expression of c-src is not necessarily correlated with cell proliferation, but suggests that, at an early stage of differentiation of mesenchymal cells, the relatively high expression of c-src could be responsible, at least in part, for the control of cell metabolism and proliferation.
D000367:Age Factors; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D005786:Gene Expression Regulation; D007700:Kinetics; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics*; D013696:Temperature; D014018:Tissue Distribution; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)