title: Cloning and physical mapping of an early region of the bacteriophage T4 genome.
abstract: We have cloned DNA restriction fragments from the largely nonessential region of bacteriophage T4 located between genes 39 and 56. The cloned DNA fragments were used to construct a precise map of the sites in this region recognized by eight restriction endonucleases. This restriction map allowed us to compare the cytosine-containing T4 DNA used for cloning with the hydroxymethylcytosine-containing DNA of wild-type T4; there were no detectable rearrangements in the region tested. We were also able to determine the physical locations of several deletion end points and of several genes.
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003596:Cytosine / Q000031:analogs & derivatives; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D013604:T-Phages / Q000235:genetics*
chemical_list: C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes