Enzyme activities in blood cells of man and dogs after separation on a discontinuous Percoll gradient.
From human and dog peripheral blood, thrombocytes (TRC), mononuclear cells (MNC), polymorphonuclear cells (PMNC), and red blood cells (RBC), were harvested after separation on a discontinuous Percoll gradient in a two-step centrifugation procedure, and 12 enzyme activities were determined in these highly purified cells. The enzyme activities measured are generally severalfold higher than previously reported, a fact which is attributed to the gentle and time-shortening isolation in Percoll, the cell disruption technique using detergent and the enzyme test conditions.
D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000201:enzymology*; D002469:Cell Separation / Q000379:methods; D002501:Centrifugation, Zonal / Q000379:methods; D003102:Colloids; D004285:Dogs; D004798:Enzymes / Q000097:blood; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000201:enzymology*; D009504:Neutrophils / Q000166:cytology / Q000201:enzymology*; D011205:Povidone; D012822:Silicon Dioxide; D013045:Species Specificity
D003102:Colloids; D004798:Enzymes; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone