Herpes simplex virus (HSV) antigens on HSV-transformed rat cells can be demonstrated by rosette formation with Staphylococcus aureus protein-A-coated sheep red blood cells.
Two rat embryo cell lines transformed by herpes simplex virus (HSV) and four tumor cell lines derived from inoculation of HSV-transformed cells formed rosettes after treatment with rabbit or human anti-HSV IgG and sheep red blood cells coated with protein A of Staphylococcus aureus, Cowan I strain. The frequency of rosette-forming cells after treatment with rabbit anti-HSV IgG at a concentration of 0.01 mg/10(6) cells varied between 41.6 and 73% depending on the cell line. Pretreatment of HSV-transformed cells with rabbit anti-HSV IgG Fab prevented rosette formation after treatment with rabbit or human anti-HSV IgG. Trypsin digestion of transformed cells reduced significantly the proportion of rosettes formed with human or rabbit anti-HSV IgG. The microscopic visualization of rosette formation on HSV-transformed cells is consistent with the expression of HSV-specific antigens on the cell surface.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D011817:Rabbits; D051381:Rats; D012397:Rosette Formation; D012756:Sheep; D018139:Simplexvirus / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A