Pattern of medium proteins radiolabelled after culture of Day 13 to 16 pig conceptus tissue with [3H]leucine and absence of chorionic gonadotrophin-like activity.
Day 13-16 pig conceptus tissue was cultured for 24 h in medium containing [3H]leucine. The patterns of radioactively labelled proteins that were released into the medium during culture were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. Day-13 conceptuses released two major radiolabelled proteins of Mr 23 000 and 26 000 and Day 14-16 conceptuses released these as well as proteins of Mr 14 000, 19 000, 44 000, 50 000 and 88 000. Various immunological and biological tests for a human chorionic gonadotrophin-like activity were performed on tissue extracts and on culture medium, but there was no evidence for its presence in the pig conceptus at Day 13-16 of gestation.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D006063:Chorionic Gonadotropin / Q000276:immunology / Q000378:metabolism; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000378:metabolism*; D005326:Fetal Proteins / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D011974:Receptors, LH; D013552:Swine; D013997:Time Factors; D014316:Tritium
D000906:Antibodies; D006063:Chorionic Gonadotropin; D005326:Fetal Proteins; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D014316:Tritium; D007930:Leucine