Macrotiter assay for coronavirus-neutralizing activity in cats using a canine continuous cell line (A-72).
A heterologous neutralization assay for feline infectious peritonitis virus serology was developed using a single continuous cell line of canine origin, A-72, which is susceptible to cytopathic infection with both transmissible gastroenteritis virus of pigs and canine coronavirus. Of several coronavirus isolates tested, the 1-71 isolate of canine coronavirus demonstrated the most effective neutralization by serum and body fluids of cats with histopathologically confirmed feline infectious peritonitis.
D000818:Animals; D002415:Cats / Q000382:microbiology*; D002460:Cell Line; D003332:Coronaviridae / Q000276:immunology*; D003333:Coronaviridae Infections / Q000276:immunology / Q000662:veterinary; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D028301:Feline Panleukopenia Virus / Q000276:immunology; D066298:In Vitro Techniques; D009500:Neutralization Tests; D010538:Peritonitis / Q000382:microbiology / Q000662:veterinary