Cloning and identification of the H-2Dp gene.
We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D006183:H-2 Antigens / Q000235:genetics*; D064532:Histocompatibility Antigen H-2D; D007739:L Cells / Q000276:immunology; D008285:Major Histocompatibility Complex; D008297:Male; D051379:Mice; D018139:Simplexvirus / Q000201:enzymology; D013094:Spermatozoa / Q000276:immunology*; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes