Demonstration of an S1-nuclease sensitive site near the human beta-globin gene, and its protection by HMG 1 and 2.
An S1-nuclease sensitive site exists within supercoiled plasmids containing the 5'-flanking sequences of the human beta-globin gene. This site is located approximately 540 base pairs upstream from the start of the gene within a region of 52 alternating purine-pyrimidine residues which has the potential to adopt either cruciform structures or Z-form DNA. This site is protected from specific cleavage by S1-nuclease by the high-mobility-group chromosomal proteins HMG1 and 2, which may be specifically acting to protect short sequences of single-stranded DNA.
D001483:Base Sequence; D001665:Binding Sites; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D004720:Endonucleases; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006609:High Mobility Group Proteins; D006801:Humans; D009690:Nucleic Acid Conformation; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013329:Structure-Activity Relationship
D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D005914:Globins; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases