[Demonstration of specific gonadotropin binding sites of chum salmon (Oncorhynchus keta) in membrane preparations of granulosa cells from the ovary of amago salmon (Oncorhynchus rhodurus)].
During late vitellogenesis, radioactive iodine labelled Salmon gonadotropin binds to theca--or granulosa--membrane preparations from amago Salmon ovary. In granulosa, native hormone inhibits iodinated GTH binding, thus indicating a specific binding (70% of total binding). In theca, a lower specific binding is found while non specific binding is higher: contamination by granulosa cells, on one hand, the possible heterogeneity of theca, on the other, could account for these results.
D000818:Animals; D001667:Binding, Competitive; D002347:Carps; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D007700:Kinetics; D010053:Ovary / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011966:Receptors, LHRH; D012474:Salmon; D013045:Species Specificity
D011956:Receptors, Cell Surface; D011966:Receptors, LHRH
Mise en évidence de sites de liaison spécifique de la gonadotropine de Saumon chum (Oncorhynchus keta) dans des préparations membranaires de granulosa d'ovaire du Saumon amago (Oncorhynchus rhodurus).