Characterisation, experimental infection and serological response to caprine retrovirus.
A virus (151) isolated from synovial membrane explant cultures from a goat with arthritis-synovitis was characterised with respect to cytopathic effect in synovial membrane cell cultures, virus morphology, buoyant density and presence of RNA dependent DNA polymerase. Virus 151 was shown to be a retrovirus with similar properties to caprine arthritis-encephalitis virus in the United States of America. Inoculation of the virus into uninfected goats caused the development of arthritis-synovitis lesions and the virus was recovered from affected joints and lung 361 days post-inoculation. The development of antibody to virus 151 was detected using an enzyme linked immunosorbent assay (ELISA). Other goats with arthritis-synovitis, progressive pneumonia or viral leukoencephalomyelitis all had antibody that reacted in this ELISA. Viruses similar to virus 151 were recovered from a number of cases. Goats inoculated with one of the viruses produced serum antibody that cross-reacted in ELISA using maedi-visna virus and virus 151 as antigens.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D001168:Arthritis / Q000662:veterinary; D003588:Cytopathogenic Effect, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats; D012190:Retroviridae / Q000302:isolation & purification / Q000472:pathogenicity; D012192:Retroviridae Infections / Q000662:veterinary*; D013585:Synovitis / Q000662:veterinary
D000914:Antibodies, Viral; D000956:Antigens, Viral