title: Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA.
abstract: The efficiency of transfection of purified HCMV genome was studied in synchronized human embryo fibroblasts. The data show that HCMV DNA infection performed in S phase is more efficient than in cells infected in G2, M and G1 phases of cell cycle, respectively. These findings stress the importance of the metabolic state of cell cultures in HCMV genome transfection.
mesh_terms: D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts; D006801:Humans; D007399:Interphase; D008938:Mitosis; D014162:Transfection
chemical_list: D004279:DNA, Viral