Human skin fibroblasts are nonpermissive to coxsackie B4 infection: an age-dependent phenomenon.
Human skin fibroblasts were previously shown to be resistant to coxsackie B4 virus infection. We have cultured fibroblasts from skin and lung tissues of donors of various ages. By a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible to coxsackie B4 infection, whereas those from older fetuses, children and adults are not and that this refractoriness is caused by a tissue-specific block to virus penetration.
D000328:Adult; D000367:Age Factors; D002478:Cells, Cultured; D002675:Child, Preschool; D029822:Enterovirus B, Human / Q000254:growth & development*; D005333:Fetus; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008168:Lung; D012867:Skin; D014776:Virus Cultivation; D014779:Virus Replication