Total synthesis and cloning of a gene coding for the ribonuclease S protein.
A gene for ribonuclease S protein, has been chemically synthesized and cloned. The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified. This flexibility facilitates tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000235:genetics*; D012260:Ribonucleases / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; C023560:ribonuclease S-peptide; D012260:Ribonucleases; D004262:DNA Restriction Enzymes