Effect of prolactin on 86Rb+ uptake, potassium content and [G-3H]ouabain binding of lactating rabbit mammary tissue.
1. Ouabain-sensitive 86Rb+ uptake in slices of lactating rabbit mammary gland significantly increased after 20 min or 1 hr of incubation with ovine prolactin (NIH-P-S12; 1 microgram/ml.). 2. Total K+ content of the tissue significantly increased at 20 min of incubation with prolactin. 3. Neither vasopressin nor oxytocin, at concentrations of 2,20 or 40 mui.u./ml., had a significant effect on ouabain-sensitive 86Rb+ uptake or total K+ of the tissue after 30 min or 1 hr of incubation. 4. Tissue slices incubated in cycloheximide at 10 micrograms/ml. for up to 260 min showed a reduction in ouabain-sensitive 86Rb+ uptake and total K+ content, with half-lives of 115 and 63 min, respectively. 5. No consistent in vitro effect of prolactin on (Na+ + K+)-activated ATPase activity in homogenates, crude microsomal fractions or NaI-activated membrane fractions from lactating rabbit mammary gland was found. 6. After 3 hr of incubation of tissue slices in the presence or absence of prolactin (5 micrograms/ml.), no significant differences in the number of [G-3H]ouabain molecules bound per cell (5.2 X 10(4) and 6.2 X 10(4), respectively) or in the dissociation constant (KD) for ouabain binding (5.4 X 10(-7) M and 5.9 X 10(-7) M, respectively) were observed. 7. Incubation of the tissue with cycloheximide (10 micrograms/ml.) for 1-6 hr caused an exponential decrease in [G-3H]ouabain binding with a half-life of 3 hr. 8. It is concluded that prolactin stimulates the activity of the (Na+ + K+)-activated ATPase in slices of lactating rabbit mammary gland within one hour but over this period does not affect the number of ouabain-binding sites, which are apparently turned over with a half-life of 1-3 hr.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007774:Lactation; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000378:metabolism*; D010121:Oxytocin / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology*; D011817:Rabbits; D011955:Receptors, Drug / Q000187:drug effects; D012413:Rubidium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors; D014667:Vasopressins / Q000494:pharmacology
D011955:Receptors, Drug; C028996:cardiac glycoside receptors; D014667:Vasopressins; D010121:Oxytocin; D010042:Ouabain; D000255:Adenosine Triphosphate; D011388:Prolactin; D003513:Cycloheximide; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D011188:Potassium