Production of leukocyte migration inhibitory factor (LIF) in human lymphocyte subsets exposed to polyclonal activators.
Lymphocyte subsets separated on the basis of nylon-wool adherence and E and EA rosetting, and characterized for the presence of esterase-positive phagocytic cells were investigated for production of leukocyte migration inhibitory factor (LIF) in response to polyclonal T- and B-cell activators, PHA, ConA, PWM, and Epstein-Barr virus (EBV). In the nylon-passed population only the high avidity E+EA+ cells responded to ConA, PHA-induced LIF production in all E-rosetting subsets. The nylon-adherent E+ subset, which contains activated T cells, produced LIF spontaneously. B cells produced LIF when exposed to PWM or uv-inactivated EBV. In accordance with the known T-cell dependence of PWM activation, LIF was detected only in supernatants of reconstituted populations containing both B and T cells. In contrast, uv-inactivated EBV, devoid of transforming potential, elicited LIF production in the pure B-cell population. LIF production in response to polyclonal activators seemed to be independent of accessory cells since reconstitution with autologous macrophages or semipurified monokine, high-molecular-weight Interleukin 1 (IL-1), did not alter the results.
D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism
D007375:Interleukin-1; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A