title: The nucleotide sequence of an unusual non-transcribed spacer and its ancestor in the rDNA in Chironomus thummi.
abstract: The nucleotide sequence of the non-transcribed spacer (NTS) in the ribosomal DNA (rDNA) of Chironomus thummi thummi and Chironomus thummi piger, including major parts of the external transcribed spacer, is described. The NTS of the two subspecies are very different in length, (thummi, 7 kb, piger, 2 kb); this is due to the insertion into the NTS of C.th. thummi of a large cluster of highly repetitive DNA sequences which are not present in the NTS of C. th. piger. The repetitive sequences, called Cla elements, are present in high copy number elsewhere in the genome of C. th. thummi and, in lower copy number, in the genome of C. th. piger in which they are mainly in the centromeric regions. Sequencing of the NTS of thummi and piger yielded information on the junctions between the Cla element cluster and the original NTS sequence, as well as on the sequence of the integration site before the transposition has occurred. The integration site is characterized by a dA cluster at the one end and a dT cluster at the other.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004275:DNA, Ribosomal; D004175:Diptera / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D004275:DNA, Ribosomal; D004247:DNA