Mode of penetration and intracellular localization of incoming parental foot and mouth disease virus (FMDV) in BHK cells.
The process of penetration and subsequent early stages of replication of Foot and Mouth Disease Virus (FMDV) in BHK21 cell cultures have been studied in order to obtain further data about the infectious cycle of this virus. Results suggest that FMDV penetrates BHK21 cells by way of pinocytic vesicles. Studies of lysosomal (LF) and supernatant (SF) fractions of homogenized suspension of infected cells were carried out to learn the percentage of possible non-specific absorption of infectious virus particles. Furthermore, analysis of intracellular virus shows that LF-associated virus is not completely degraded by the enzymatic process expressed by cellular proteases and RNAses and can be potentially useful for genetic expression. Data suggest that: i) only a part of LF-associated virus replicates; ii) the remaining LF-associated virus may be a reservoir of potentially active virus.
D000818:Animals; D005537:Aphthovirus / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D007668:Kidney; D012367:RNA, Viral / Q000096:biosynthesis; D013347:Subcellular Fractions / Q000382:microbiology; D013997:Time Factors; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D012367:RNA, Viral; D014529:Uridine