The reactivity of Reed-Sternberg cells with monoclonal antisera at thin section and ultrastructural levels.
In an attempt to improve the morphology of Reed-Sternberg cells after immunochemical procedures, the biotin-avidin technique has been used. Satisfactory morphology was obtained at the level of both light and electron microscopy. Reed-Sternberg cells failed to react with a monoclonal antimonocyte serum but gave positive results with anti-1a and FMC7, a monoclonal anti-B cell serum. Positive results were also obtained with FMC1 and B1 both of which are monoclonal B cell antisera.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D006684:HLA-DR Antigens; D006644:Histiocytes / Q000276:immunology* / Q000648:ultrastructure; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006689:Hodgkin Disease / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II