A comparison of the mutagenicity and macromolecular binding of the carcinogens Michler's ketone and reduced Michler's ketone.
The mutagenicity of 4,4'-bis(dimethylamino)benzophenone (Michler's ketone, MK, CAS No. 90-94-8) and 4,4'-methylenebis(N,N-dimethyl)benzamine (reduced Michler's ketone, RMK, CAS No. 101-61-1) for Salmonella typhimurium strain TA100 was compared using activated 9000 X g (S9) liver supernatants from 2 animal species. RMK, but not MK, was mutagenic when incubated with phenobarbital-induced B6D2F1 mouse or Osborne-Mendel rat-liver S9. The mutagenic response for RMK was linear at doses from 1 to 33 micrograms/plate. A higher percentage of RMK and MK became irreversibly bound to mouse-liver macromolecules than to rat-liver macromolecules when incubated at 37 degrees C in the presence of reduced nicotinamide adenine dinucleotide phosphate.
D000814:Aniline Compounds / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001559:Benzhydryl Compounds / Q000378:metabolism / Q000633:toxicity*; D001577:Benzophenones / Q000378:metabolism / Q000633:toxicity*; D004247:DNA / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics
D000814:Aniline Compounds; D001559:Benzhydryl Compounds; D001577:Benzophenones; D009153:Mutagens; C014682:Michler's ketone; D009249:NADP; D004247:DNA; C035472:N,N,N',N'-tetramethyl-4,4'-methylenedianiline