Isolation of deuterated histones from yeast grown on media dissolved in 2H2O.
We have succeeded in growing Saccharomyces cerevisiae (baker's yeast) on media containing 2H2O and isolating the core histones highly deuterated in the non-exchangeable positions. The deuterated histones obtained here are of great value for their possible widespread use for structural studies of chromatin.
D003903:Deuterium; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006657:Histones / Q000235:genetics / Q000302:isolation & purification*; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
D006657:Histones; D003903:Deuterium