Photoaffinity inhibition of peptide transport in yeast.
A photoaffinity label, 4-azidobenzoyltrimethionine has been synthesized. It competitively inhibits trimethionine uptake in the yeast C. albicans. Upon UV irradiation it irreversibly and specifically blocks oligopeptide uptake. These results give the first example of photoinhibition of peptide uptake in yeast.
D000345:Affinity Labels / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009842:Oligopeptides / Q000378:metabolism*; D010782:Photolysis
D000345:Affinity Labels; D001386:Azides; D009842:Oligopeptides; C022877:trimethionine; C037458:4-azidobenzoyltrimethionine