title: Methods of increasing the sensitivity of the haemagglutination inhibition test for rabies virus antibody.
abstract: A new method for the removal of non-specific inhibitors of rabies virus haemagglutinin has been developed. Treatment with colloidal silicic acid (Aerosil) or with acetone plus Aerosil reduced the non-specific inhibitors in human, mouse, and dog sera to a level that was undetectable at the 1:4 starting dilution in the haemagglutination inhibition test.Bromelain-treated goose erythrocytes were much more susceptible to haemagglutination by rabies virus than were untreated erythrocytes, and the sensitivity of the haemagglutination inhibition test was considerably increased by using bromelain-treated erythrocytes. Low levels of antibodies in sera from immunized human subjects were detected with higher sensitivity by combining Aerosil treatment of the sera with the use of bromelain-treated goose erythrocytes in the haemagglutination inhibition test.
mesh_terms: D000096:Acetone / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001963:Bromelains / Q000494:pharmacology; D003102:Colloids; D004285:Dogs; D004912:Erythrocytes / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006801:Humans; D007616:Kaolin / Q000494:pharmacology; D051379:Mice; D011817:Rabbits; D011818:Rabies / Q000175:diagnosis*; D011820:Rabies virus / Q000276:immunology*; D012824:Silicic Acid / Q000494:pharmacology
chemical_list: D000914:Antibodies, Viral; D003102:Colloids; D012824:Silicic Acid; D000096:Acetone; D007616:Kaolin; D001963:Bromelains