[Cytophotometric study of the granules formed in frog erythrocytes incubated in solutions of acridine orange, chloroquine and daunorubicin].
Granules arising in the cytoplasm of Rana temporaria erythrocytes incubated in either solution containing acridine orange, chloroquine or antibiotic daunorubicine were studied cytophotometrically. The stuff concentrations in the granules were estimated under various conditions of incubation. The stuff concentrations in the granules appeared to be 1000-fold higher than in the incubation solutions. The average concentration in the granules did not depend on either the initial concentration of the stuff solution or the incubation time. The stuff concentration in the granules decreases with the increase of the granule size. Mechanisms of cation-acid segregation and accumulation by living cell are discussed.
D000165:Acridine Orange / Q000494:pharmacology*; D000818:Animals; D002738:Chloroquine / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000187:drug effects* / Q000648:ultrastructure; D003630:Daunorubicin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D010783:Photometry; D011896:Rana temporaria; D012996:Solutions; D013997:Time Factors
D012996:Solutions; D002738:Chloroquine; D000165:Acridine Orange; D003630:Daunorubicin
Tsitofotometricheskoe issledovanie granul, obrazuiushchikhsia v éritrotsitakh liagushki pri inkubatsii ikh v rastvorakh akridinovogo oranzhevogo, khlorokhina i daunorubitsina.