Production of interleukin 2 in serum-free medium.
The production of interleukin 2 by rat splenocytes, stimulated with Con A in serum-free medium, was studied. Under optimal conditions the stimulation in serum-containing and serum-free media resulted in identical interleukin 2 titers. The addition of phorbol myristate acetate prior or simultaneously with Con A did not augment interleukin 2 production. In contrast, preincubation of lymphocytes in serum-free medium for 24-36 hrs brought about a 8-10-fold increase of interleukin 2 titer. The interleukin 2 titer 4 hrs after the induction of preincubated lymphocytes with Con A was about 20% of maximal activity. The possible mechanism of early interleukin 2 production is under discussion.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D007376:Interleukin-2 / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate