The 19-kDal protein encoded by early region 1b of adenovirus type 12 is synthesized efficiently in Escherichia coli only as a fused protein.
We have constructed recombinant plasmids that direct the synthesis of the Mr 19 000 protein encoded by the adenovirus type 12 (Ad12) E1b region as either a native protein or a protein fused to the amino-terminal portion of the elongation factor EF-TuB in Escherichia coli cells. Using these recombinants, we could synthesize a large amount of the fused protein, while only a small amount of the native Mr 19 000 protein was produced. The failure to synthesize the native Mr 19 000 protein in E. coli cells was ascribed to inefficient translation.
D000256:Adenoviridae / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008242:Lysogeny; D010957:Plasmids; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins