Activation of macrophages by culture fluid of antigen-stimulated spleen cells collected from chickens immunized with Eimeria tenella.
The effect of spleen cell factors on the activation of macrophages was investigated in chickens immunized with Eimeria tenella. The abilities of peritoneal macrophages obtained from normal chickens to kill sporozoites of E. tenella and to inhibit intracellular development of Toxoplasma gondii were enhanced by exposing them to 33 and 50% culture fluid of antigen-stimulated spleen cells of chickens immunized with E. tenella. The parasiticidal activity of normal macrophages was also distinctly enhanced by the treatment with culture fluid of phytohemagglutinin-stimulated normal spleen cells. On the other hand, the parasiticidal activity of normal macrophages could not be enhanced by the treatment with culture fluid of antigen-stimulated normal spleen cells under conditions similar to those of culture fluid of antigen-stimulated immune spleen cells. It thus appears that the macrophage activator was induced from immune spleen cells in response to the stimulation by the antigen.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D004539:Eimeria / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D008222:Lymphokines / Q000276:immunology; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology
D000941:Antigens; D008222:Lymphokines; D016215:Macrophage-Activating Factors