title: Tetanus toxin binding to different morphological phenotypes of cultured rat and bovine adrenal medullary cells.
abstract: Tetanus toxin (TT) binding to cultured rat and bovine adrenal medullary cells has been investigated using indirect immunofluorescence and anti-dopamine-beta-hydroxylase (DBH) antibodies as a probe to identify catecholaminergic cells. TT binds to all rat adrenal medullary cells which display a neuronal phenotype induced by treatment with nerve growth factor and/or medium conditioned by C6 glioma cells. In contrast, 90-95% of the rounded DBH-positive cells are TT-negative, suggesting that in vitro-transdifferentiation of rat chromaffin cells alternates the expression of membrane properties. Cultured bovine chromaffin cells have no TT binding sites independent of their morphological phenotype.
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004299:Dopamine beta-Hydroxylase / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D009414:Nerve Growth Factors; D010641:Phenotype; D051381:Rats; D013744:Tetanus Toxin / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009414:Nerve Growth Factors; D013744:Tetanus Toxin; D004299:Dopamine beta-Hydroxylase