Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein.
A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.
D002364:Caseins; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D006868:Hydrolysis; D006923:Hymecromone / Q000031:analogs & derivatives; D007700:Kinetics; D046911:Macromolecular Substances; D010447:Peptide Hydrolases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014468:Umbelliferones
C037949:4-methylumbelliferyl-casein; D002364:Caseins; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D014468:Umbelliferones; D006923:Hymecromone; D010447:Peptide Hydrolases