Rapid immunoperoxidase assay for detection of respiratory syncytial virus in nasopharyngeal secretions.
Samples of nasopharyngeal secretions obtained from 70 infants and young children with acute respiratory disease were examined for the presence of respiratory syncytial virus by immunoperoxidase assay (IPA). The IPA was compared with the immunofluorescence assay and with cell culture isolation. Respiratory syncytial virus antigen-positive cells were detected by both IPA and immunofluorescence assay in 28 specimens; 25 samples were positive in cell culture. The agreement between virus isolation and IPA and IFA was 89%. The applicability of IPA to rapid viral diagnosis of respiratory syncytial virus infection is discussed.
D002648:Child; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D009305:Nasopharynx / Q000378:metabolism / Q000382:microbiology*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*
D006735:Horseradish Peroxidase