[Restriction analysis of the plasmid and chromosomal DNA of the strain Streptomyces fradiae--a producer of neomycin].
Plasmid DNA with a molecular weight of 53-55 Md was isolated from Streptomyces fradiae strain 676 producing neomycin on centrifugation of the DNA preparation at the density gradient of cesium chloride-ethidium bromide. The extrachromosomal DNA had 6 recognition sites for Bgl II, more than 13 recognition sites for Bam HI, more than 14 recognition sites for Kpn I, more than 18 recognition sites for Pst I, more than 20 recognition sites for Sac I, more than 21 recognition sites for Pvu II and no recognition sites for Eco RI, Eco RV, Hind III and Sla I.
D055598:Chemical Phenomena; D002621:Chemistry; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D009355:Neomycin / Q000096:biosynthesis*; D010957:Plasmids; D013045:Species Specificity; D013302:Streptomyces / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D009355:Neomycin
Restriktsionnyĭ analiz plazmidnoĭ i khromosomnoĭ DNK shtamma Streptomyces fradiae--produtsenta neomitsina.