Influence of water quality on enteric virus concentration by microporous filter methods.
Four enteric viruses, poliovirus type 1, echovirus type 1, reovirus type 3, and simian adenovirus SV-11, were concentrated from seeded 1.3-liter volumes of raw, finished, and granular activated carbon-treated waters by adsorption to 47-mm-diameter (17 cm2), electropositive ( Virosorb 1MDS ) filters at pH 7.5 or electronegative ( Filterite ) filters at pH 3.5 with and without 5 mM added MgCl2, followed by elution with 0.3% beef extract in 50 mM glycine at pH 9.5. Removal of particulates from raw and finished waters by 0.2-micron prefiltration before virus addition and pH adjustment had little effect on virus concentration efficiencies. Soluble organic compounds reduced virus adsorption efficiencies from both raw and finished waters compared with granular activated carbon-treated water, but the extent of interference varied with virus type and adsorption conditions. For electropositive 1MDS filters, organic interference was similar with all virus types. For Filterite filters, organic interference was evident with poliovirus and echovirus, but could be overcome by adding MgCl2. Reovirus and SV-11 were not adversely affected by organics during adsorption to Filterite filters. Elution of reovirus and adenovirus was inefficient compared with that of poliovirus and echovirus. None of the three adsorption schemes ( 1MDS at pH 7.5 and Filterite with and without 5 mM MgCl2 at pH 3.5) could be judged superior for all viruses and water types tested.
D000256:Adenoviridae / Q000302:isolation & purification*; D000261:Adenoviruses, Simian / Q000302:isolation & purification*; D000327:Adsorption; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008274:Magnesium; D015636:Magnesium Chloride; D012089:Mammalian orthoreovirus 3 / Q000302:isolation & purification*; D008851:Micropore Filters; D017955:Poliovirus / Q000302:isolation & purification*; D012087:Reoviridae / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods; D014867:Water / Q000032:analysis; D014871:Water Microbiology
D015636:Magnesium Chloride; D014867:Water; D008274:Magnesium