The isolation and immunochemical characterisation of a cell-wall carbohydrate and a membrane lipocarbohydrate antigen of group B streptococcus, type II.
Two distinct carbohydrate antigens were isolated from the cell surface of group B streptococcus, type II. One antigen was extracted from SDS-purified cell walls by cold trichloroacetic acid and contained galactose, glucose, rhamnose, glucosamine and sialic acid in the approximate molar proportions 1.7:1.0:3.4:0.9:0.21 respectively. The serological activity of this polymer indicated that it is the group-specific antigen common to all group B streptococci. The second antigen was extracted by phenol from cell membranes and contained galactose, glucose, glucosamine, phosphorus and fatty acid in a molar ratio of 1.6:1.0:0.35:2.6:0.016 respectively. This antigen was shown to be specific for type II, group B streptococcus.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D013292:Streptococcus agalactiae / Q000032:analysis* / Q000276:immunology
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial