Long-range conformational transition in yeast tRNAPhe, induced by the Y-base removal and detected by chloroacetaldehyde modification.
Chemical modification was used to study the conformational changes occurring in yeast tRNAPhe after the Y-base excision. The chemical probe was the adenine- and cytosine-specific reagent chloroacetaldehyde. Comparison of the modification patterns in tRNAPhe and tRNAPhe-Y shows that seven bases, adenines 35, 36 and 38 in the anticodon loop and adenines 73, 76 and cytosines 74, 75 in the 3'-terminus were modified in both tRNAs with a quantitative difference in the modification level of the anticodon loop bases. The most interesting, however, is the qualitative difference consisting in modification of cytosine-60 in the T psi C loop of tRNAPhe-Y. Some aspects of the mechanism of this long-distance conformational transition are briefly discussed.
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D001483:Base Sequence; D006147:Guanine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation / Q000187:drug effects; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012346:RNA, Transfer, Amino Acyl; C012099:wye; D006147:Guanine; C004656:chloroacetaldehyde; D000079:Acetaldehyde