Evaluation of a new ELISA method for estimating rubella-specific IgM antibody.
Results of using a new enzyme-linked immunosorbent assay (ELISA) kit for detecting rubella IgM antibodies were compared with those obtained by fractionating samples of serum on sucrose density gradients followed by rubella haemagglutination inhibition tests on the fractions. There was close agreement between the two methods. A major advantage of the ELISA method was the ability to complete tests within one day.
D000914:Antibodies, Viral / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012409:Rubella / Q000175:diagnosis*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic