[Changes in the cellular localization of gamma-crystallins in the lens differentiation process in amphibia].
The lenses were studied in the Rana temporaria embryos by means of immunochemistry from the formation of lens vesicle till the end of metamorphosis. The rabbit antisera against gamma- and beta-crystallins of Rana temporaria were used. The materials were fixed by acetone or Carnois fixative. beta-crystallins were shown to appear simultaneously with gamma-crystallins at stage 31--32 (Dabagyan, Sleptzova, 1975). gamma-Crystallins appeared first in the lens fibers and later (stages 36--51) in the epithelial cells. From stage 52 (tail reduction) the immunofluorescence of gamma-crystallins in the epithelium fell sharply until full disappearance. At stage 54 (end of metamorphosis) a weak fluorescence of gamma-crystallins was found in the anterior part of epithelium only. gamma-Crystallins were not found in the epithelium of adult frogs.
D000327:Adsorption; D000663:Amphibians / Q000196:embryology*; D000818:Animals; D003459:Crystallins / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007908:Lens, Crystalline / Q000196:embryology* / Q000378:metabolism; D011896:Rana temporaria; D012995:Solubility
D003459:Crystallins
Izmenenie kletochnoĭ lokalizatsii gamma-kristallinov v protsesse differentsirovki khrustalika amfibiĭ.