title: Receptor-mediated regulation of calcium mobilization and cyclic GMP synthesis in neuroblastoma cells.
abstract: In neuroblastoma N1E 115 cells, carbachol, histamine and PGE1 elevated cyclic GMP content and, induced the efflux of preloaded 45Ca2+, the release of membrane-bound Ca2+ measured by fluorescent CTC, and the increase in [Ca2+]i as measured by Quin 2 fluorescence. The time course of the responses, the absolute requirement of extracellular Ca2+, the inhibition by receptor blockers, and the concentration dependency on histamine were all similar between these responses. The observation indicates that the mobilization of Ca2+, especially the increase of [Ca2+]i, may be intimately linked to the synthesis of cyclic GMP in the cells.
mesh_terms: D000527:Alprostadil; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D006152:Cyclic GMP / Q000096:biosynthesis* / Q000502:physiology; D006632:Histamine / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000502:physiology; D013050:Spectrometry, Fluorescence
chemical_list: D007473:Ion Channels; D011458:Prostaglandins E; D011956:Receptors, Cell Surface; D006632:Histamine; D002217:Carbachol; D000527:Alprostadil; D006152:Cyclic GMP; D002118:Calcium