Stably denatured regions in chromosomal DNA from the cdc2 Saccharomyces cerevisiae cell cycle mutant.
DNA isolated from Saccharomyces cerevisiae strains carrying temperature-sensitive mutations in the CDC2 gene after incubation at the restrictive temperature contains multiple stably denatured regions 200 to 700 base pairs long. These regions are probably stabilized by a DNA-binding protein. They are found in both replicated and unreplicated portions of DNA molecules, suggesting that they are not an early stage in the initiation of DNA replication.
D002453:Cell Cycle; D002875:Chromosomes / Q000032:analysis; D004261:DNA Replication; D004271:DNA, Fungal / Q000302:isolation & purification*; D005800:Genes, Fungal; D009154:Mutation; D009691:Nucleic Acid Denaturation; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics
D004271:DNA, Fungal