[Increased yields of insulin by recycling of non-combined insulin chains].
The yield of insulin by the combination of reduced A and B chains is increased from 15% to 33-34% if the non-insulin-containing fractions of the gel filtration are isolated and used for further combinations (recycling). Crude insulin recovered by gel filtration can be highly purified by preparative high-performance liquid chromatography. Lower yields of insulin were obtained on recombination of chains which had been recycled five times or more. Sulfur analyses show a loss of cystine sulfur. The occurrance of lanthionine and lysinoalanine indicates an alkaline destruction of cystine/cysteine during the repeated combination steps.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D013552:Swine
D000596:Amino Acids; D007328:Insulin
Erhöhte Insulinausbeuten durch "Recycling" der nichtkombinierten Ketten.