Template-independent synthesis of guanosine tetra- and pentaphosphates on ribosomes.
It is shown that Escherichia coli ribosomes carrying poly(Lys)-tRNA can form (p)ppGpp in the presence of stringent factor in the absence of the poly(A) template. Template-independent synthesis of (p)ppGpp is suppressed by tetracycline and partially decreases if deacylated tRNA is omitted.
D004926:Escherichia coli / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000096:biosynthesis*; D006158:Guanosine Pentaphosphate / Q000096:biosynthesis*; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis*; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013698:Templates, Genetic; D013752:Tetracycline / Q000494:pharmacology
D006150:Guanine Nucleotides; D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl; D011061:Poly A; D006159:Guanosine Tetraphosphate; D006158:Guanosine Pentaphosphate; D010444:Peptide Elongation Factor Tu; D013752:Tetracycline