Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.
A three-base substitution mutant of the yeast tRNALeu3 has been constructed. The mutation, introduced through the use of a heptadecanucleotide as a site-specific mutagen, is localized in the anterior portion of the promoter and results in the inability to form a D stem. The mutant is active in transcription, but maturation of the 5' terminus and splicing are abolished. The results are discussed in the light of a recently proposed model for initiation of transcription of eucaryotic tRNA genes.
D005796:Genes; D007930:Leucine; D009154:Mutation; D009694:Nucleic Acid Precursors / Q000378:metabolism; D010957:Plasmids; D012323:RNA Processing, Post-Transcriptional; D012343:RNA, Transfer / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012343:RNA, Transfer; D007930:Leucine