The role of products of the human HLA-DR locus (Ia molecules) in in vitro M. leprae driven lymphoproliferation.
We analysed the role of Ia molecules in T cell activation with M. leprae by using two hybridoma monoclonal antibodies D1-12 and D4-22 with specificity for non-polymorphic isotypes NG-1 and NG-2 respectively on the HLR-DR molecular framework. The results show that the addition of either monoclonal antibody up to 3 days after onset of culture significantly blocks the in vitro M. leprae driven lymphoproliferation. The mechanism of suppression appears to be due to a combination of steric hindrance and a vigorous suppressor signal from anti-Ia treated macrophages. The possible implications of these results are discussed.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D009166:Mycobacterium leprae / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II