Control of rat C6 glioma cell proliferation: uncoupling of the inhibitory effects of hydrocortisone hormone in suspension and monolayer cultures.
We undertook a comparative study of the effects of the hormone hydrocortisone (Hy) on C6 glioma cells grown in monolayer and in suspension in cultures. We found Hy reversibly renders C6 cells anchorage- and serum-dependent for their growth. In monolayer cultures, Hy was found to inhibit cell cycle traversing exclusively at G1 phase. In agarose suspension, Hy was found to block colony development. Hy-resistant variants were selected and isolated in agarose suspension. Examination of these variants showed that cells selected for Hy-resistance in suspension can be Hy sensitive when anchored to a solid substrate. We conclude that resistance to Hy in suspension and resistance to it in monolayer culture are two independent phenotypes.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003584:Cytological Techniques; D004351:Drug Resistance; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006854:Hydrocortisone / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D009374:Neoplasms, Experimental / Q000188:drug therapy; D051381:Rats
D006854:Hydrocortisone