title: Capacity of different cell types to prime in vivo for secondary in vitro cytotoxic T-cell responses against non-major-histocompatibility antigens.
abstract: The capacity of several types of cell preparations to induce in vivo a state of memory for a secondary in vitro cytotoxic response against non-major-histocompatibility antigen was markedly reduced (on a per cell basis) by uv-irradiation. This indicated that memory induction requires metabolically active stimulator cells. An "adherent cell preparation" (AC) that was enriched for dendritic cells was among the most effective memory-inducing cell populations; but concanavalin A-activated nylon-wool-nonadherent spleen cells (Con A-NWT) or concanavalin A-activated unfractionated spleen cells (Con A-spl) were on the average equally effective. Normal unfractionated spleen cells (spl) or nonactivated nylon-wool-nonadherent cells (NWT) were markedly less effective on a per cell basis. This pattern of stimulatory activity was in line with the relative stimulatory activity of these cell types in primary cytotoxic responses in the presence of interleukin 2 (IL-2) and also in line with the relative capacity to induce IL-2-dependent proliferation in H-2D-incompatible T-cell populations (cf. W. Dröge et al., J. Immunol. 132, 2749, 1984). These differences in the immunogenic potential and the requirement for metabolically active stimulator cells suggested that these cells stimulated the CTL system directly and not indirectly through antigen processing cells of the immunized host. Nevertheless, the secondary cytotoxic response after injection of low numbers of Con A-spl into H-2 heterozygous recipients, (BALB/c X BALB/b)F1, or into recipients with recombinant H-2 haplotype (A.J) was only preferentially but not exclusively restricted to the H-2 haplotype of the immunizing cell populations. Restriction was considerably more complete when AC were used for immunization.
mesh_terms: D000818:Animals; D002460:Cell Line; D003208:Concanavalin A; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens / Q000276:immunology*; D007156:Immunologic Memory; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000528:radiation effects; D013945:Thymoma / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology
chemical_list: D006649:Histocompatibility Antigens; D007376:Interleukin-2; D003208:Concanavalin A