Multiple, tandem plasmid integration in Saccharomyces cerevisiae.
Nonreplicating plasmids transform Saccharomyces cerevisiae by recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.
D004261:DNA Replication; D008040:Genetic Linkage; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*