Immunohistochemical localization of corticotropin-releasing factor (CRF)-like immunoreactivity in the hypothalamus of the newt, Triturus cristatus.
The distribution of corticotropin-releasing factor (CRF) immunoreactivity was investigated in the hypothalamus and preoptic area of the newt by single and double immunocytochemical procedures. CRF immunopositive cell bodies were seen in the preoptic area (from the anterior wall of the preoptic recess to the dorsal parts of the preoptic nucleus) and in the tuberal portions of the posterior hypothalamus. Abundant nerve fibres are seen in the outer zone of the median eminence, while the pars nervosa lacks CRF-immunoreactivity. CRF immunoreactive material is seemingly separated from neurophysins- and somatostatin immunoreactive cell bodies and fibres.
D000818:Animals; D003346:Corticotropin-Releasing Hormone; D006651:Histocytochemistry / Q000379:methods; D007031:Hypothalamus / Q000378:metabolism*; D007034:Hypothalamus, Posterior / Q000378:metabolism; D007120:Immunochemistry / Q000379:methods; D008473:Median Eminence / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000378:metabolism; D011301:Preoptic Area / Q000378:metabolism; D014319:Triturus / Q000378:metabolism*
D010455:Peptides; D003346:Corticotropin-Releasing Hormone