Developmental expression of chicken fetal antigen on peritoneal macrophage: implications for hematopoietic differentiation.
Chicken fetal antigen (CFA) was demonstrated by both complement-mediated microcytotoxicity and immunofluorescence to be present on the cell surface of activated peritoneal macrophage. While CFA-positive macrophage were observed in young birds, the antigen was absent from adult macrophage. A comparison of cell surface CFA on macrophage, lymphocytes, and erythrocytes indicated that unique subsets of CFA determinants are characteristic for each differentiation lineage. As a result, CFA can serve as a cell surface marker for differentiation-specific gene expression. Since CFA determinants are known to be at least partly defined by carbohydrates, it is suggested that hematopoietic heterogeneity for CFA may result from lineage-specific carbohydrate microheterogeneity.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002454:Cell Differentiation; D002645:Chickens; D003602:Cytotoxicity, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D006410:Hematopoiesis; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; C041136:Ia-like antigens, avian