title: Effect of human saliva and serum on ion transport by dog tracheal mucosa. Comparison of normal subjects with cystic fibrosis patients.
abstract: It has been proposed that cystic fibrosis (CF) saliva or serum contains factors that alter ion transport in various tissues. Since CF frequently affects the lungs, and disturbances in epithelial ion transport has been proposed to contribute to the pathophysiology of pulmonary disease in CF, we investigated whether saliva or serum from CF patients could alter ion transport across airways epithelium. Canine tracheal mucosae were mounted in lucite chamber and perfused on both sides with Krebs-Henseleit solution at 37 degrees C and pH 7.4. Unidirectional fluxes of 22Na and 36Cl were measured in pairs of mucosal tissues under short circuit conditions. Saliva from 5 CF patients stimulated short circuit current (SCC) by 33 +/- 8 microA/cm2 and net Cl secretion by 1.10 +/- 0.33 muEq/cm2 X h (mean +/- SE; p less than 0.05). No change occurred in net Na absorption. Saliva from normal subjects raised SCC by 34 +/- 7 microA/cm2 and increased net Cl secretion by 1.29 +/- 0.46 muEq/cm2 X h (n = 9; p less than 0.02). Serum from both CF patients and control subjects briefly stimulated SCC. Exposure of tracheal mucosa to normal as well as CF saliva, such as by aspiration, or to serum, such as by transudation, stimulates Cl secretion, and therefore water secretion into airway lumen. There appears to be no difference in the effect exerted by CF saliva or serum and that of normal subjects. These findings argue against the presence of a specific circulating or secreted CF factor that could alter ion transport across respiratory epithelia in a way that might contribute to lung disease in CF.
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002712:Chlorides / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D004285:Dogs; D004553:Electric Conductivity; D005260:Female; D006801:Humans; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D012463:Saliva / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism; D000516:alpha-Amylases / Q000494:pharmacology
chemical_list: D002712:Chlorides; D012964:Sodium; D000516:alpha-Amylases