Vertical sections of cultivated anchorage-dependent cells for electron microscopy.
A method is described for electron microscopic preparation of cultivated cells for vertical sections using the test chamber system TCSC-1. The cells are cultivated on a special foil. They can be fixed and embedded directly in the chamber. After polymerization of the resin, the foil can be easily taken off and a second resin layer is poured upon the embedded cells. Then distinct cells can be marked under an inverted light microscope. The bilayer of the resin allows optimal conditions for vertical sections of anchorage-dependent cells.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured / Q000648:ultrastructure*; D003584:Cytological Techniques / Q000295:instrumentation; D008099:Liver / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains