The use of cellular reagents as an adjunct to typing for HLA-DRw10.
Two human T cell lines recognizing DRw10 were generated. One line was directly alloreactive, while a second cloned line recognized mumps virus in the context of a DRw10-like determinant. In panel studies, the lines responded only to DRw10 cells, and not to DR1 (DRw10-) cells. When compared to 5 anti-DRw10 sera, the cellular reagents gave virtually identical results. The generation and use of such cellular reagents may be of particular value for the identification of HLA-D-region specificities that are rare, poorly defined, or for which serologic typing reagents are limited.
D002460:Cell Line; D006684:HLA-DR Antigens; D015798:HLA-DR1 Antigen; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009109:Mumps virus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D006684:HLA-DR Antigens; D015798:HLA-DR1 Antigen; C043263:HLA-DRw10; D000949:Histocompatibility Antigens Class II