Aminopeptidases and proteolipids of intestinal brush border.
The presence of human blood group A determinants has been shown on the A+ rabbit intestinal brush border glycoproteins, particularly hydrolases. Sugar compositions of aminopeptidases N from A+ and A- rabbits were compatible with the presence in these molecules of eight N-linked glycans and of two O-linked glycans bearing the A determinants in A+ animals. The exact relative molecular masses of hydrophobic domain(s) of aminopeptidases N and A from pig and rabbit intestinal brush border have been determined by an isotopic dilution technique. The values obtained were compatible with the anchorage in the membrane of the monomeric rabbit enzymes, or of each subunit of the dimeric pig enzymes, by their N-terminal sequences, composed of 20-25 hydrophobic amino acids. This N-terminal hydrophobic sequence (14 residues) has been determined for rabbit aminopeptidase N. Short peptides containing approximately 60% hydrophobic amino acids have been extracted by chloroform-methanol from purified brush border and basolateral membranes of pig enterocytes. Their molecular properties were very similar to those of the aminopeptidase anchors released by trypsin treatment of detergent-extracted enzymes. However, several lines of evidence failed to support the assumption that these free hydrophobic peptides can be identified with anchors left inside the bilayer after proteolytic cleavage of surface hydrolases.
D000017:ABO Blood-Group System; D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D004285:Dogs; D006801:Humans; D007421:Intestine, Small / Q000032:analysis / Q000201:enzymology* / Q000276:immunology; D008871:Microvilli / Q000032:analysis / Q000201:enzymology / Q000276:immunology; D011510:Proteolipids / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D013552:Swine
D000017:ABO Blood-Group System; D011510:Proteolipids; D000626:Aminopeptidases