Enzymes of malate oxidation in Mycobacterium leprae grown in armadillo livers.
A NAD-dependent malate dehydrogenase is the principal enzyme for malate oxidation by Mycobacterium leprae, FAD-dependent malate-vitamin K reductase was detected at about 1% the level of the NAD-dependent activity. Both enzyme activities were detected in extracts from M. leprae treated with NaOH to abolish host-derived activities which might be adsorbed to the bacteria and the NAD-dependent enzyme was shown to be electrophoretically distinct from the host-tissue enzyme, thus establishing that these were both authentic bacterial enzymes. Mycobacterium leprae does not possess malic enzyme.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001135:Armadillos; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology / Q000382:microbiology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D009166:Mycobacterium leprae / Q000201:enzymology* / Q000302:isolation & purification; D010084:Oxidation-Reduction
D008293:Malates; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; C021527:malate-vitamin K oxidoreductase