High levels of heat labile Escherichia coli enterotoxin in bacterial cell sonicates of agar grown cultures.
Escherichia coli heat-labile enterotoxin from 5 strains of human and animal origin was detected in broth cultures and agar-plate culture sonicates by using CHO and Y-1 adrenal cells and rabbit intestinal loop assay. Cell sonicates possessed higher levels of heat-labile enterotoxin than supernatants of broth cultures. Highest levels of heat-labile (LT) enterotoxin were detected in the sonicates of cultures grown on blood agar, followed by those grown on MacConkey, and lactose agar respectively.
D000362:Agar; D001427:Bacterial Toxins; D001769:Blood; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000254:growth & development; D029968:Escherichia coli Proteins; D007785:Lactose; D013010:Sonication
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D000362:Agar; C027538:heat-labile enterotoxin, E coli; D007785:Lactose