P22 antirepressor protein prevents in vivo recA-dependent proteolysis of P22 repressor.
A method was developed to demonstrate recA-dependent P22-repressor breakdown in vivo by SDS-polyacrylamide electrophoresis of unfractionated extracts of phage-infected, lysogenic Salmonella typhimurium strains TA1530 rec+ and TA1530 recA1-. The antirepressor of P22 is not cleaved by recA protein. Under conditions of unregulated ant-overproduction (Harvey et al. 1981) antirepressor protects c2-repressor in vivo against proteolytic cleavage by recA protein.
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D006868:Hydrolysis; D011938:Rec A Recombinases; D012097:Repressor Proteins / Q000037:antagonists & inhibitors*; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000235:genetics*; D014157:Transcription Factors / Q000037:antagonists & inhibitors*
D001426:Bacterial Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D011938:Rec A Recombinases