A rapid procedure for the preparation of highly purified pyruvate decarboxylase from brewer's yeast.
A rapid purification procedure for pyruvate decarboxylase (E.C. 4.1.1.1.) from fresh cells of brewer's yeast (Saccharomyces carlsb.) is reported. The preparation of a crude enzyme (30-45 U/mg) by the use of fractionation steps with protamine sulfate, acetone, and ammonium sulfate takes about 6-7 h. A stable pyruvate decarboxylase (70-85 U/mg) was obtained from such preparations after purification on CM Sephadex C 50 after another 2-3 h. Stability and structural properties are compared for enzymes prepared from fresh and dried yeast.
D002262:Carboxy-Lyases / Q000302:isolation & purification*; D004355:Drug Stability; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D011767:Pyruvate Decarboxylase / Q000302:isolation & purification* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D046911:Macromolecular Substances; D002262:Carboxy-Lyases; D011767:Pyruvate Decarboxylase