[Free radical oxidation of lipids and erythrocyte stability to hemolysis in healthy and sick children].
Simultaneous estimation of malonic dialdehyde (MDA) and intensity of hemolysis in erythrocytes under conditions of Fe2+-ascorbate dependent lipid peroxidation (LP) as well as after addition of alpha-tocopherol and ergocalciferol into the samples was carried out in healthy children and adults as well as in prematurely born children and in children with diseases of kidney and of cardiovascular system. Distinct accumulation of MDA was observed in erythrocytes of prematurely born children and of children with kidney and cardiovascular system diseases, whereas the intensity of erythrocyte hemolysis was increased only in the cases of cardiovascular diseases. Initiation of LP by means of Fe2+-ascorbate increased the rate of erythrocyte hemolysis and MDA content only when their initial level was low. alpha-Tocopherol decreased the LP rate and did not affect the erythrocyte stability in healthy persons; in erythrocytes of diseased children it often stimulated the cell hemolysis but not affect the LP rate. Ergocalciferol exhibited the distinct pro-oxidant effect and increased the erythrocyte hemolysis in healthy persons; in the diseased children both these effects of the vitamin were decreased.
D000293:Adolescent; D000328:Adult; D002318:Cardiovascular Diseases / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004872:Ergocalciferols / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals; D005921:Glomerulonephritis / Q000097:blood; D006461:Hemolysis; D006801:Humans; D007223:Infant; D008054:Lipid Peroxides / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D014810:Vitamin E / Q000494:pharmacology
D004872:Ergocalciferols; D005609:Free Radicals; D008054:Lipid Peroxides; D014810:Vitamin E; D008315:Malondialdehyde
Svobodnoradikal'noe okislenie lipidov i ustoĭchivost' k gemolizu éritrotsitov zdorovykh i bol'nykh deteĭ.