Chloride transport properties of human leukemic cell lines K562 and HL60.
The Cl- transport characteristics of the human leukemic cell lines K562 and HL60, with erythroid and granulocytic phenotypic features, respectively, were investigated. Cl- effluxes were measured with 36Cl- under equilibrium conditions in both cell lines and were found to be three orders of magnitude slower than the unidirectional efflux of Cl- in normal erythrocytes. Induction of differentiation of the K562 cell line with hemin does not affect the rate of Cl- transport, while induction of the HL60 cell line with dimethyl sulfoxide results in a small decrease in the rate of Cl- transport. Cl- transport in both cell lines could be divided into two components. One component is inhibited by treatment with 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), displays counter-transport characteristics, and has a high energy of activation--all properties characteristic of the human erythrocyte-facilitated anion exchange system. The second component is insensitive to DIDS, is partially inhibited by furosemide, and has a low energy of activation.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives*; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000345:Affinity Labels / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D002713:Chlorine; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D011197:Potassium Radioisotopes; D011868:Radioisotopes; D012979:Sodium Radioisotopes
D000345:Affinity Labels; D002712:Chlorides; D007473:Ion Channels; D011197:Potassium Radioisotopes; D011868:Radioisotopes; D012979:Sodium Radioisotopes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D002713:Chlorine; C018564:dihydro-DIDS; D006427:Hemin; D005665:Furosemide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D004121:Dimethyl Sulfoxide