title: Determination of N tau-methylhistamine in urine by gas chromatography using nitrogen-phosphorus detection.
abstract: The determination of N tau-methylhistamine in urine, using gas chromatography with nitrogen-phosphorus detection and the homologue N tau-ethylhistamine as internal standard, is described. A comparison between the present method and a previously described stable isotope dilution mass fragmentographic method resulted in a regression line of Y = 0.023 + 0.944X mumol/l with a correlation coefficient of 0.996. The 24-h excretions of 35 normal adults on a free diet ranged from 0.4 to 1.8 mumol. Patients with mastocytosis, chronic myelocytic leukemia, anaphylactic reactions and a patient after bronchial provocation showed above normal values.
mesh_terms: D000707:Anaphylaxis / Q000652:urine; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006632:Histamine / Q000378:metabolism*; D006801:Humans; D007201:Indicator Dilution Techniques; D007951:Leukemia, Myeloid / Q000652:urine; D008761:Methylhistamines / Q000652:urine*; D014582:Urticaria Pigmentosa / Q000652:urine
chemical_list: D008761:Methylhistamines; D006632:Histamine