Similar binding of the carcinostatic drugs cis-[Pt(NH3)2Cl2] and [Ru(NH3)5Cl] Cl2 to tRNAphe and a comparison with the binding of the inactive trans-[Pt(NH3)2Cl2] complex - reluctance in binding to Watson-Crick base pairs within double helix.
A comparative study of the binding of square planar cis- and trans-[Pt(NH3)2Cl2] complexes and the octahedral [Ru(NH3)5(H2O)]3+ complex to tRNAphe from yeast was carried out by X-ray crystallography. Both of the carcinostatic compounds, cis-[Pt(NH3)2Cl2] and [Ru(NH3)5(H2O)]3+ show similarities in their mode of binding to tRNA. These complexes bind specifically to the N(7) positions of guanines G15 and G18 in the dihydrouridine loop. [Ru(NH3)5(H2O)]3+ has an additional binding site at N(7) of residue G1 after extensive soaking times (58 days). A noncovalent binding site for ruthenium is also observed in the deep groove of the acceptor stem helix with shorter (25 days) soaking time. The major binding site for the inactive trans-[Pt(NH3)Cl2] complex is at the N(1) position of residue A73, with minor trans-Pt binding sites at the N(7) positions of residues Gm34, G18 and G43. The similarities in the binding modes of cis-[Pt(NH3)2Cl2] and [Ru(NH3)5(H2O)]3+ are expected to be related to their carcinostatic properties.
D001482:Base Composition; D055598:Chemical Phenomena; D002621:Chemistry; D002945:Cisplatin; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl; D012428:Ruthenium; D017975:Ruthenium Compounds; D012441:Saccharomyces cerevisiae; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D012346:RNA, Transfer, Amino Acyl; D017975:Ruthenium Compounds; C039419:pentaammineaquaruthenium; C039420:pentaamminechlororuthenium; D012428:Ruthenium; D002945:Cisplatin