Different responses elicited in vitro by antibodies to IgM and IgD in cells from a surface IgM + D-positive human follicular lymphoma.
The effect of anti-Igs in combination with the tumour promotor TPA on DNA and Ig synthesis in a human follicular germinal centre cell lymphoma carrying sIgM and sIgD was investigated. The lymphoma cells responded to both anti-mu and anti-delta chains and to anti-lambda light chains by DNA synthesis, as measured by methyl-(3H)-thymidine incorporation. Flow cytofluorometric measurements, however, showed that only anti-mu chains induced marked increase in cytoplasmic Ig content. This result suggests that in certain B-cell subsets the signals elicited via sIgM and sIgD are different.
D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007072:Immunoglobulin D / Q000096:biosynthesis / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology*; D008224:Lymphoma, Follicular / Q000276:immunology*; D009194:Myeloma Proteins / Q000494:pharmacology; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D009194:Myeloma Proteins; D011947:Receptors, Antigen, B-Cell; D004247:DNA; D013755:Tetradecanoylphorbol Acetate