Effect of adenosine deaminase and an adenosine analogue on insulin sensitivity in soleus muscle of the rat.
The concentration of insulin that produces half-maximal stimulation of glycolysis in stripped soleus muscle preparations is decreased from approximately 100-10 muunits/ml by the presence of adenosine deaminase in the incubation medium. This suggests that adenosine decreases insulin sensitivity. The effect of the deaminase is abolished by addition of the adenosine analogue, N6-phenylisopropyladenosine which is not metabolised by the deaminase. The effect of the deaminase in isolated soleus muscle is similar to that of a period of physical training of the rat.
D000241:Adenosine / Q000031:analogs & derivatives*; D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D006003:Glycogen / Q000096:biosynthesis; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009700:Nucleoside Deaminases / Q000378:metabolism*; D010660:Phenylisopropyladenosine / Q000494:pharmacology*; D051381:Rats
D007328:Insulin; D007773:Lactates; D010660:Phenylisopropyladenosine; D019344:Lactic Acid; D006003:Glycogen; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine