Murine granulopoiesis after fractionated total lymphoid irradiation and allogeneic bone marrow transplantation.
We investigated the effects of fractionated total lymphoid irradiation (TLI) and allogeneic bone marrow transplantation on murine granulopoiesis in order to evaluate the hemopoietic microenvironment of radiation chimeras (RC). BALB/c mice received 3400 rad TLI (17 daily 200 rad fractions) with or without 3 X 10(7) C57Bl/Ka marrow cells injected intravenously. Radiation resulted in prolonged depression of granulocyte-macrophage progenitor cells (CFU-GM) and endosteal colony-stimulating-activity (CSA) production in irradiated humeri. Allogeneic marrow transplantation partially restored endosteal CSA production and led to complete, although delayed, restoration of CFU-GM. Major compensatory granulopoiesis occurred in the spleen. Marrow fat-laden adherent cells (FLAC) were cultured in vitro from RC 30 weeks post TLI and transplantation. As determined by indirect immunofluorescence utilizing anti-H-2 antibodies, 23-25% of these cells reacted with antibodies possessing donor specificity. These findings suggest that the hemopoietic microenvironment, represented functionally by endosteal CSA production and morphologically by cultured FLAC, is transplantable by the intravenous route.
D000273:Adipose Tissue / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D016026:Bone Marrow Transplantation; D002448:Cell Adhesion; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology*; D006410:Hematopoiesis; D008208:Lymphatic System / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D003115:Colony-Stimulating Factors