title: Autoimmune damage to spermatogenesis in rodents immunized with mouse F9 embryonic carcinoma cells.
abstract: Significant inhibition of spermatogenesis and appearance of antibodies against spermatogenic cells identified by cytotoxicity and immunofluorescence reactions were observed in mice of inbred strains 129/Sv and BALB/c and in albino guinea pigs after syngeneic, allogeneic, and xenogeneic immunization with mouse F9 embryonic carcinoma cells and Freund's complete adjuvant. A similar syngeneic immunization with PYS-2 cells was ineffective. Appropriate absorption experiments confirmed the similarity between the antigens of F9 and spermatogenic cells and the absence of such a similarity with antigens of PYS-2 cells. These results support the hypothesis that the oncofetal F9 antigens represent spermatogenic differentiation antigens and thus play an essential role in spermatogenic cell differentiation.
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000276:immunology; D013091:Spermatogenesis; D013724:Teratoma / Q000276:immunology*; D013737:Testis / Q000276:immunology
chemical_list: D001323:Autoantibodies