title: Factors regulating the specific activity of galactose-1-phosphate uridyltransferase during perfusion of suckling rat liver.
abstract: The regulation of the specific activity of galactose-1-phosphate uridyltransferase (E.C.2.7.7.12) has been studied using the hexose-perfused suckling rat liver as a model. Perfusion in the recirculating mode with galactose concentrations up to 4mM resulted in enzyme activity which fluctuated significantly during a 90-min experimental period. A concentration of 10 mM galactose stabilized the enzyme activity at levels found in unperfused liver. The same stabilizing effect was seen with 10 mM glucose. The elevated transferase specific activity observed after 30 min recirculating perfusion was not seen when the nonrecirculation mode was employed suggesting a factor in the recirculating medium may mediate this effect.
mesh_terms: D000828:Animal Population Groups / Q000502:physiology*; D000818:Animals; D000833:Animals, Suckling / Q000502:physiology*; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C037363:UDPgalactose - UDP-N-acetylglucosamine galactosephosphotransferase; D005947:Glucose; D005690:Galactose