Evaluation of di-(2-ethylhexyl)phthalate and its major metabolites in the Ames test and L5178Y mouse lymphoma mutagenicity assay.
Di-(2-ethylhexyl)phthalate (DEHP) and its two major metabolites, mono-(2-ethylhexyl)phthalate (MEHP) and 2-ethylhexanol (EH), were tested for genetic activity in both the Salmonella/mammalian microsome mutagenicity (Ames) assay and the L5178Y TK+/- mouse lymphoma cell mutagenicity assay. All chemicals were tested in both the presence and absence of Aroclor-induced liver microsomes prepared from male Sprague-Dawley rats. Dose levels for both assays were selected from preliminary toxicity studies for each chemical. Neither DEHP, MEHP, nor EH exhibited any significant mutagenic activity in strains TA-98, TA-100, TA-1535, TA-1537, and TA-1538 in the Ames test or when tested in the L5178Y TK+/- mouse lymphoma cell mutagenicity assay.
D000818:Animals; D001711:Biotransformation; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives / Q000633:toxicity*; D000441:Hexanols / Q000633:toxicity*; D007940:Leukemia L5178 / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics*; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D010795:Phthalic Acids / Q000633:toxicity*; D010968:Plasticizers; D012486:Salmonella typhimurium / Q000187:drug effects; D013329:Structure-Activity Relationship
D000441:Hexanols; D009153:Mutagens; D010795:Phthalic Acids; D010968:Plasticizers; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate; C034017:2-ethylhexanol