Transplantation of T-lymphocyte-depleted bone marrow between HLA-mismatched individuals.
Four patients with acute leukemia received transplants from HLA-mismatched, related donors. Marrow cells that had been depleted of T lymphocytes using a monoclonal anti-T-lymphocyte antibody and rabbit complement were used. In vitro studies showed that 80-97% of the mature T lymphocytes were removed using this procedure. Infusion of the treated marrow was accomplished without complications, and engraftment occurred in each case. Graft-versus-host disease occurred in 3 of the 4 patients. These results show that additional manipulations of the marrow will be required to allow complete T lymphocyte removal from the marrow before the feasibility of this approach in HLA-mismatched patients can be determined.
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D016026:Bone Marrow Transplantation; D002648:Child; D003114:Colony-Forming Units Assay; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D006086:Graft vs Host Disease / Q000517:prevention & control; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007258:Informed Consent; D007938:Leukemia / Q000628:therapy; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D003165:Complement System Proteins