Release of diacylglycerol moieties from various glycosyl diacylglycerols.
Periodic acid oxidation in methanol followed by incubation with 1, 1-dimethylhydrazine results in release of diacylglycerols from 1,2-diacyl-3-glycosyl-sn-glycerols. During hydrazinolysis of oxidized monogalactosyl diacylglycerol, an intermediate hydrazone derivative was observed which was isolated and identified. The diacylglycerols recovered are 1,2-diacyl isomers containing the same fatty acid mixtures as the intact glycolipids. The yields of diacylglycerols released from plant monogalactosyl-, digalactosyl-, and sulfoquinovosyl diacylglycerols were in the range of 30-50%. The method may be used for analysis of molecular species and for preparative purposes.
D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000032:analysis*; D004127:Dimethylhydrazines; D038983:Galactolipids; D005989:Glycerides / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010712:Phosphatidic Acids / Q000032:analysis; D010715:Phosphatidylglycerols / Q000032:analysis; D010944:Plants
D004075:Diglycerides; D004127:Dimethylhydrazines; D038983:Galactolipids; D005989:Glycerides; D006017:Glycolipids; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; C008543:galactosylglyceride; C013694:sulfoquinovosyl diglyceride; D010504:Periodic Acid