title: Induction of germinal vesicle breakdown in Xenopus laevis oocytes: response of denuded oocytes to progesterone and insulin.
abstract: Denuded oocytes freed of their vitelline envelope have been prepared by two methods, enzymatically with pronase and manually by microdissection. The response of denuded oocytes to progesterone, in terms of germinal vesicle breakdown (GVBD), was similar to that obtained with defolliculated oocytes (separated with collagenase from follicle cells, but still keeping their vitelline membrane). The same conclusion was drawn with respect to morphological features of the oocyte surface observed by transmission and scanning electron microscopy, before and after progesterone-induced GVBD. The synergistic effect of insulin and progesterone in denuded oocytes was comparable to that observed in defolliculated oocytes. Multiplication stimulating activity (MSA) had the same effect as insulin. These observations indicate that hormones act directly upon oocytes, without interference of the surrounding vitelline envelope and follicle cells.
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II; D007700:Kinetics; D008854:Microscopy, Electron; D009865:Oocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000502:physiology; D010455:Peptides / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D014817:Vitelline Membrane / Q000502:physiology; D014982:Xenopus laevis
chemical_list: D007328:Insulin; D010455:Peptides; D011374:Progesterone; D007335:Insulin-Like Growth Factor II