Production of antibodies by inoculation into lymph nodes.
The intra-lymph node technique used to inoculate rabbits with small quantities of antigen has been described. A variety of antigens in the 20,000-22,000 molecular weight range, as well as a 15-amino acid peptide coupled to BSA, have been inoculated successfully by this procedure. We have made no attempt to compare the success rate of the intra-lymph node inoculation route with other techniques utilizing small (microgram) quantities of antigen.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D005260:Female; D005620:Freund's Adjuvant; D013006:Growth Hormone / Q000032:analysis / Q000276:immunology; D006728:Hormones / Q000032:analysis*; D006801:Humans; D007158:Immunologic Techniques; D008198:Lymph Nodes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D011388:Prolactin / Q000032:analysis / Q000276:immunology; D011817:Rabbits / Q000276:immunology
D000941:Antigens; D006728:Hormones; D011388:Prolactin; D013006:Growth Hormone; D005620:Freund's Adjuvant