Glucose concentration and insulin release in 5-thio-D-glucose-treated mice.
Male albino mice were given a single dose of various concentrations (25, 50, and 100 mg/kg body weight) of 5-thio-D-glucose or daily infusions (33 mg/kg body weight) of 5-thio-D-glucose for 21 days. Elevated blood glucose and immunoreactive insulin (IRI) levels were observed in the mice treated with 5-thio-D-glucose. Fasting glucose levels reached a maximum in 30 minutes and IRI levels reached a maximum in 60 to 90 minutes in the single-dose treated animals compared to preintubation levels. In the mice treated for 21 days, the fasting and fed glucose and IRI levels were significantly increased. Single dose of glucose (1 g/kg body weight) given to fasting and fed mice did not alter the glucose and IRI levels in the treated animals. However, a single dose of 5-thio-D-glucose (33 mg/kg body weight) given to fasting and fed treated animals increased the IRI levels significantly but not the glucose concentration. These data show that both single-dose and 3-week treatment with 5-thio-D-glucose produced a hyperinsulinemic diabetes in male albino mice.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight; D004305:Dose-Response Relationship, Drug; D005215:Fasting; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D051379:Mice; D011863:Radioimmunoassay; D013997:Time Factors
D001786:Blood Glucose; D007328:Insulin; C003002:5-thio-D-glucose; D005947:Glucose