Nitrocellulose membrane as an antigen or antibody carrier for screening hybridoma cultures.
Nitrocellulose membranes were used as carriers of antigen for rapid screening of specific monoclonal antibodies or as carriers of the monoclonal antibody (in a crude form) for a simple and economic determination of isotype of the antibody by a modification of enzymoimmunoassay.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000941:Antigens / Q000276:immunology; D001666:Binding Sites, Antibody; D002352:Carrier Proteins / Q000276:immunology*; D002482:Cellulose / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007126:Immunoglobulin Allotypes / Q000032:analysis; D007330:Insulin Antibodies / Q000032:analysis; D008566:Membranes / Q000276:immunology; D051379:Mice; D013552:Swine
D000911:Antibodies, Monoclonal; D000941:Antigens; D002352:Carrier Proteins; D007126:Immunoglobulin Allotypes; D007330:Insulin Antibodies; D002482:Cellulose