S-acylated residues of the acyl-carrier protein subunit of Klebsiella aerogenes citrate lyase.
Oxidation of the isolated deacetyl acyl-carrier protein subunit of citrate lyase from Klebsiella aerogenes with Cu2+-o-phenanthroline complex leads exclusively to intrapeptide disulfide bridge formation indicating that the cysteamine and the cysteine residues are located in close proximity. The S-acetylation of the cysteine residue in deacetyl acyl-carrier protein subunit is catalysed by a citrate lyase ligase preparation in presence of acetate and ATP. Reaction-inactivation of citrate lyase results in deacetylation of the S-acetyl cysteamine residue of the prosthetic group but not of the S-acylated cysteine residue in the acyl-carrier protein.
D000213:Acyl Carrier Protein / Q000302:isolation & purification / Q000378:metabolism*; D000215:Acylation; D002250:Carbon Radioisotopes; D019730:Carbon-Sulfur Ligases; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008025:Ligases / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D010084:Oxidation-Reduction; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D010618:Phenanthrolines / Q000378:metabolism; D011485:Protein Binding
D000213:Acyl Carrier Protein; D002250:Carbon Radioisotopes; D009097:Multienzyme Complexes; D010618:Phenanthrolines; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase; D008025:Ligases; D019730:Carbon-Sulfur Ligases; C019908:citrate (pro-3S)-lyase ligase; C025205:1,10-phenanthroline