title: Gastric juice electrolyte secretion in conscious dogs with gastric fistulae and its modification by FPL 52694, a mast cell stabilizing agent.
abstract: Studies have been made of the electrolyte output in the gastric juice of conscious dogs equipped with gastric fistulae during stimulation by intravenous infusion of either pentagastrin (2 micrograms kg-1 h-1), histamine (30 micrograms kg-1 h-1) or insulin (0.1 u kg-1 h-1). The mast cell stabilizing agent, FPL 52694 (4.35 mg ml-1) was instilled into the stomach for 30 min and caused a marked reduction of H+ output, H+ concentration and osmotic strength of the juice during stimulation with pentagastrin, histamine, or insulin. There was also a marked increase in the rate of Na+ output into the juice. When pentagastrin-stimulated acid secretion was inhibited by cimetidine (4 mumol kg-1 i.v.) acid output was reduced but there were no sustained changes in ion concentrations, osmolarity or Na+ output of the type seen following inhibition with FPL 52694. It is concluded that FPL 52694 may have a dual mode of action in this preparation; a direct reduction of the output of hydrochloric acid and a smaller effect to increase gastric NaHCO3 output leading to a post-secretory neutralization of the juice.
mesh_terms: D000818:Animals; D002867:Chromones / Q000494:pharmacology*; D002927:Cimetidine / Q000494:pharmacology; D004285:Dogs; D004573:Electrolytes / Q000378:metabolism*; D005747:Gastric Fistula; D005750:Gastric Juice / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D010418:Pentagastrin / Q000494:pharmacology
chemical_list: D002867:Chromones; D004573:Electrolytes; D007328:Insulin; C029061:FPL-52694; D002927:Cimetidine; D006632:Histamine; D010418:Pentagastrin