Influence of misonidazole on survival of wild-type and radiosensitive mutants of yeast.
Survival curves were obtained for haploid and diploid yeasts, Saccharomyces cerevisiae, of a wild-type strain and radiosensitive mutants exposed to gamma-rays in oxygenated and hypoxic conditions both in the absence and in the presence of misonidazole. Misonidazole enhanced the radiosensitivity only of hypoxic cells. A correlation between oxygen and misonidazole sensitization was observed. The data confirm that misonidazole mimics the sensitizing effect of oxygen. The degree of liquid-holding recovery of yeast cells was greater when the cells were irradiated in hypoxic conditions with than without misonidazole. Possible reasons for the observed radiation responses are discussed.
D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D006238:Haploidy; D000860:Hypoxia; D008920:Misonidazole / Q000494:pharmacology*; D009593:Nitroimidazoles / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000528:radiation effects
D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; D008920:Misonidazole