Influence of proteolysis inhibitors and immunoadsorption technique on the composition of rabbit antigen-specific receptor preparations. Presence of Fc receptors.
Addition of proteolysis inhibitors during the isolation procedure of rabbit arsanylated bovine IgG specific receptors decreased significantly the amount of low-molar-mass proteins in all receptor preparations. Rabbit ARS-BGG-specific receptor preparations isolated by immunoadsorption technique contain molecules which do not react with antigen and antibodies against immunoglobulins and have identical molar mass and chymotryptic peptide composition as those of isolated Fc receptors. It is suggested that during isolation of antigen-specific receptors from the surface of lymphoid cells, Fc receptors react with complexes composed of antigen and Ig+ receptors on the surface of immunoadsorbent and are isolated together with antigen-specific receptors.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D002469:Cell Separation; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D008214:Lymphocytes / Q000276:immunology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D011817:Rabbits; D011946:Receptors, Antigen / Q000276:immunology / Q000302:isolation & purification*; D011961:Receptors, Fc / Q000032:analysis* / Q000276:immunology; D013450:Sulfones / Q000494:pharmacology*
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D011946:Receptors, Antigen; D011961:Receptors, Fc; D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride