Specific quantitation of secretory immunoglobulin A with enzyme immunoassay using activated thiol--Sepharose for separation method.
A specific and sensitive enzyme immunoassay system for human secretory IgA was developed using anti-alpha-chain antibodies coupled to activated thiol-Sepharose, and anti-secretory component antibodies labeled with beta-D-galactosidase from Escherichia coli. The dose response of the enzyme activity in eluate was observed between 3 and 1000 ng of secretory IgA with little cross-reactions with IgA, IgG, IgM and secretory component. The assay method could be employed for the measurement of secretory IgA in saliva, urine, feces, intestine and serum without interferences by the abundant IgA in the same samples.
D000818:Animals; D002675:Child, Preschool; D003429:Cross Reactions; D005243:Feces / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007223:Infant; D007231:Infant, Newborn; D007422:Intestines / Q000276:immunology; D009369:Neoplasms / Q000276:immunology; D011134:Polysaccharides / Q000494:pharmacology*; D011817:Rabbits; D012463:Saliva / Q000276:immunology; D012685:Sepharose / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014556:Urine / Q000032:analysis
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D011134:Polysaccharides; D013438:Sulfhydryl Compounds; D012685:Sepharose