Coding assignments of each mRNA synthesized in vitro and protein components of CPV of silkworm Bombyx mori.
The coding properties of each mRNA synthesized in vitro of CPV have been examined. The mRNAs were separated by polyacrylamide gel electrophoresis and isolated from the gel slices. The individual mRNA was then translated into the wheat germ cell-free protein synthesizing system. The products of translation were analyzed by the immuno-reaction with the 125I-IgG of each of the five electrophoretic protein components of CPV. The coding assignments were deduced on the basis of the immuno-reaction as follows: mRNA1, mRNA3 and mRNA9 encode group P1 of viral proteins, mRNA2 and mRNA6 encode group P2 and P3, respectively. mRNA7 and mRNA8 encode group P4 while mRNA9 encode group P5. There are more than 10 polypeptides in the CPV virion as demonstrated by two-dimensional electrophoresis.
D000818:Animals; D012831:Bombyx / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005815:Genetic Code; D007074:Immunoglobulin G / Q000032:analysis; D007304:Insect Viruses / Q000378:metabolism*; D010452:Peptide Biosynthesis; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012087:Reoviridae / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis*
D007074:Immunoglobulin G; D012333:RNA, Messenger; D014764:Viral Proteins