[Isolation of a mutant of influenza virus A/PR8/34 (H1N1) and its properties].
A mutant causing small plaques in chick embryo cells was isolated from influenza virus A/PR8/34 (H1N1). The diameter of the plaques remained constant over 39 passages. Virus titers in 8-11-day-old chick embryos were so low, that positive hemagglutination results could be recorded only in 35% of the inoculated eggs. There was no increase in virus titer after repeated passages in chick embryos. The sensitivity of the mutant to nonspecific serum and tissue inhibitors was higher than that of the initial strain. Mice survived intranasal inoculation with the mutant. The immunofluorescent antibody technique allowed the visualization of virus antigen in the lung of infected mice.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology; D001790:Blood Physiological Phenomena; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008168:Lung / Q000276:immunology; D051379:Mice; D009154:Mutation; D009976:Orthomyxoviridae Infections / Q000276:immunology; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D000998:Antiviral Agents
Isolierung einer Mutante des Influenzavirus A/PR8/34 (H1N1) und ihre Eigenschaften.