Production of interleukin 2 containing growth medium for cloning of human T lymphocytes.
Culture conditions which would enhance production of interleukin 2 (IL-2) from PHA-stimulated peripheral blood mononuclear cells (PBM) were studied. One thousand rads. irradiation of the cells was found to alter the kinetics and to increase the production of IL-2. Addition of indomethacin or irradiated EBV-transformed B lymphocytes to the cultures, removal of adherent cells, and 18 h preincubation of the cells before PHA stimulation had an additive enhancing effect on the IL-2 production. Following these studies a simple and efficient method producing IL-2 for cloning of human T cells was developed.
D001402:B-Lymphocytes; D002448:Cell Adhesion / Q000187:drug effects; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000378:metabolism* / Q000528:radiation effects; D003470:Culture Media / Q000494:pharmacology; D004854:Herpesvirus 4, Human; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D003470:Culture Media; D007376:Interleukin-2; D007213:Indomethacin