Mutagenicity of styrene on metabolizing D7 strain of saccharomyces cerevisiae.
The level of cyt.p-450 in the D7 strain of the yeast S.cerevisiae depended on the substrate supporting the growth, on its concentration, on the starting inoculated number of cells. (1) In the yeast grown on D-mannose where fermentation and respiration occurred concomitantly, cytochrome P-450 was also formed. It was detected a maximal concentration during the logarithmic phase when in the cultures there are about 50 . 10(6) cell/ml. We use cells harvested at this moment of the growth for mutagenesis tests. The tested substances were dimethylnitrosamine and styrene. DMNA to probe the sensibility of our cells and styrene that has always given contrasting results but from which the formation is known of genetically active metabolite: styrene oxide(6-7). Styrene gave positive results with our metabolizing yeast cells.
D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D020058:Styrene; D013343:Styrenes / Q000633:toxicity*
D003470:Culture Media; D009153:Mutagens; D013343:Styrenes; D020058:Styrene; D003577:Cytochrome P-450 Enzyme System; D004128:Dimethylnitrosamine