Quantitation of haptens by homogeneous immunoprecipitation. 2. Centrifugal analysis for tobramycin, phenobarbital, and theophylline in serum.
Our homogeneous immunoprecipitation inhibition assay (Clin. Chem. 28:659-661, 1982) is applied here to tobramycin, phenobarbital, and theophylline. Only 10-50 microL of test sample is needed. No sample treatment, dilution, or extraction is required. A test serum sample is simultaneously mixed with a drug conjugate and its specific antiserum in a centrifugal analyzer. The subsequent reaction and the measurement are completed in 3 min. Within-run and between-run CVs for clinically relevant concentrations were well below 10%. Results for patients' samples correlated well with those by enzyme immunoassay.
D000900:Anti-Bacterial Agents / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D002498:Centrifugation; D011232:Chemical Precipitation; D003429:Cross Reactions; D006241:Haptens / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics; D010634:Phenobarbital / Q000097:blood*; D013053:Spectrophotometry; D013806:Theophylline / Q000097:blood*; D013997:Time Factors; D014031:Tobramycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D000936:Antigen-Antibody Complex; D006241:Haptens; D013806:Theophylline; D014031:Tobramycin; D010634:Phenobarbital