title: Completion of the amino acid sequence of the alpha 1 chain from type I calf skin collagen. Amino acid sequence of alpha 1(I)B8.
abstract: The complete amino acid sequence of the 279-residue CNBr peptide CB8 from the alpha 1 chain of type I calf skin collagen is presented. It was determined by sequencing overlapping fragments of CB8 produced by Staphylococcus aureus V8 proteinase, trypsin, Endoproteinase Arg-C and hydroxylamine. Tryptic cleavages were also made specific for lysine by blocking arginine residues with cyclohexane-1,2-dione. This completes the amino acid sequence analysis of the 1054-residues-long alpha (I) chain of calf skin collagen.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003094:Collagen; D019811:Hydroxylamine; D006898:Hydroxylamines; D010446:Peptide Fragments / Q000032:analysis* / Q000302:isolation & purification; D010447:Peptide Hydrolases; D012867:Skin / Q000032:analysis*; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D006898:Hydroxylamines; D010446:Peptide Fragments; D019811:Hydroxylamine; D003094:Collagen; D010447:Peptide Hydrolases