A method for the isolation of intact, viable forespores from Bacillus species using high pressure.
Large-scale preparation of Bacillus forespores was performed using nitrogen gas under high pressure to force an osmotically stable spheroplast suspension through a micrometer needle valve. Ninety-nine percent recovery of intact viable forespores at various stages of sporulation was achieved with a variety of Bacillus species. The isolated forespores are stable for up to 7 days when kept under nongerminating conditions.
D001407:Bacillus / Q000502:physiology*; D001431:Bacteriological Techniques / Q000295:instrumentation; D008858:Microscopy, Phase-Contrast; D011312:Pressure; D013171:Spores, Bacterial / Q000302:isolation & purification