Measurement of specific antibodies in human intestinal aspirate: effect of the protease inhibitor phenylmethylsulphonyl fluoride.
Incorporation of the protease inhibitor phenylmethylsulphonyl fluoride in a solid-phase enzyme-linked immunoassay increased the sensitivity of this assay for detecting antibodies to dietary proteins present in human intestinal aspirate. Its effect was due in part to the prevention of antigen degradation.
D000328:Adult; D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex; D002446:Celiac Disease / Q000276:immunology; D002648:Child; D004044:Dietary Proteins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000276:immunology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D012016:Reference Values; D013450:Sulfones / Q000494:pharmacology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D004044:Dietary Proteins; D007106:Immune Sera; D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride