Primary response to GAT in F344 rats: anti-GAT antibodies, nonspecific immunoglobulins, and expression of the GAT-13 idiotype.
It has been reported that antigen induces differentiation of two populations of Ig-containing cells: the first one to appear, IgCC, synthesizes nonspecific Ig and the second, AbCC, synthesizes antibodies. Along with other arguments, the observation that nonspecific Ig bear idiotypic determinants, which cross-react with those of antibodies, had led to the hypothesis that IgCC are precursors of AbCC. However, the synthesis of such idiotype-positive nonspecific Ig before the appearance of the antibodies has not yet been proven. This problem was investigated by analyzing the primary response to poly(Glu60-Ala30-Tyr10) (GAT) in F344 rats. Kinetics studies of cells synthesizing Ig expressing a major idiotype (GAT-13), and of cells synthesizing Ig not expressing GAT-13 idiotype, revealed that these two cell populations were undetectable before the appearance of the anti-GAT antibodies. This demonstrates that IgCC differentiation is not a necessary condition for the development of all antibody responses.
D000818:Animals; D000917:Antibody Formation; D006462:Hemolytic Plaque Technique; D006735:Horseradish Peroxidase / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D051381:Rats; D011916:Rats, Inbred F344
D007130:Immunoglobulin Idiotypes; D007136:Immunoglobulins; D010455:Peptides; D011108:Polymers; C022204:anti-GAT antibodies, idiotypic; C020749:GAT; D006735:Horseradish Peroxidase