[Circulating immune complexes: optimization and characteristics of the C1q(I125) fixation test].
The present paper describes the optimisation of a liquid phase [125I] C1q binding assay for the detection of circulating immune complexes. Blood must be collected without any anticoagulant. The serum can be stored or transported as usual without damage. A 90 min incubation is needed to reach the equilibrium of the reaction; polyethylene glycol (2,5% final concentration) is added to precipitate the [125I] C1q-CIC complex. By its simplicity and reproducibility, this test can be currently employed, especially in the case of treatment by plasmapheresis.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D003168:Complement Fixation Tests / Q000379:methods*; D006801:Humans; D007457:Iodine Radioisotopes
D000936:Antigen-Antibody Complex; D007457:Iodine Radioisotopes; D015922:Complement C1q; D003166:Complement Activating Enzymes
Complexes immuns circulants: optimisation et caractéristiques d'un test de fixation du C1q [125I].