Retrovirus RD114 p30 is bound by the extracellular matrix proteins, fibronectin and laminin.
Purified retrovirus RD114 p30 was found to bind to the extracellular matrix proteins, fibronectin and laminin. The purified matrix proteins were immobilized onto polystyrene and the binding of p30 was quantitated using radioiodinated proteins and enzyme immunoassay (EIA). The dissociation constants were for the p30-fibronectin binding Kd = 5.3 X 10(-8) M and for the p30-laminin Kd = 7.3 X 10(-8) M. The molecular ratio in the binding from 4000 ng/ml of p30 was 1.9 mol per mol fibronectin and 2.5 mol per mol laminin. The interaction between fibronectin and RD114 retrovirus was also detected using a modified immunoblotting procedure.
D005353:Fibronectins / Q000378:metabolism*; D015683:Gene Products, gag; D007797:Laminin / Q000378:metabolism*; D008970:Molecular Weight; D012190:Retroviridae / Q000032:analysis*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000378:metabolism*
D005353:Fibronectins; D015683:Gene Products, gag; D007797:Laminin; D014763:Viral Matrix Proteins; D014764:Viral Proteins