The 1-nitropyrene reductase of Salmonella typhimurium.
We have devised a sensitive fluorimetric assay to monitor the conversion of 1-nitropyrene to 1-aminopyrene. Application of this assay to extracts of Salmonella typhimurium strains TA98 and TA100 (which are sensitive to the mutagenic and lethal effects of 1-nitropyrene) has shown that these bacteria contain 'nitropyrene reductase' activity at the low level of 10(-11) mol/min/mg protein. NADPH and NADH serve equally well as reducing agents. The nitropyrene reductase activity of strain TA100 F50 (a mutant resistant to 1-nitropyrene) was found to be considerably lower.
D000632:Aminopyrine / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009601:Nitroreductases; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism; D012486:Salmonella typhimurium / Q000201:enzymology*; D013050:Spectrometry, Fluorescence
D011721:Pyrenes; D000632:Aminopyrine; D009243:NAD; D009249:NADP; D010088:Oxidoreductases; C037214:1-nitropyrene reductase; D009601:Nitroreductases