A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E.
A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.
D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis*; D007163:Immunosorbent Techniques; D010940:Plant Proteins; D011058:Pollen / Q000276:immunology*; D011863:Radioimmunoassay
D000485:Allergens; C026967:Amb a I protein, Ambrosia artemisiifolia; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D010940:Plant Proteins; D007073:Immunoglobulin E