title: 140 000 Dalton surface glycoprotein. A plasma membrane component of the detergent-resistant cytoskeletal preparations of cultured human fibroblasts.
abstract: A 140 000 D glycoprotein (140 kD gp), labelled radioactively with surface-specific techniques, remained as the major cell surface glycoprotein in the detergent-resistant cytoskeletal preparations of cultured human fibroblasts. The 140 kD gp was present also in trypsinized cells and was not affected by treatment of the cells either with collagenase, chymotrypsin or thrombin. In density gradient fractionation of whole cells the 140 kD gp was recovered in the plasma membrane fraction together with small amounts of cytoskeletal components. In fractionation of cytoskeletal preparations, on the other hand, the 140 kD gp could not be dissociated from cytoskeletal proteins and together with vimentin it formed the major component of the oligomeric polypeptide complex generated by treating the surface-labelled cytoskeletal preparations with bifunctional cross-linking reagent, dithiobis succinimidyl propionate (DTPS). Moreover, the 140 kD gp seemed to copurify with vimentin upon reconstitution of intermediate filaments from urea-solubilized cytoskeletal preparations. On the other hand, low ionic-induced degradation of vimentin led to a decrease in the amount of the detergent-resistant 140 kD gp on the cell surface. In electron microscopy, a close apposition between bilayer-like plasma membrane remnants of the adherent cytoskeletons and cytoskeletal elements could be seen. The results indicate that the 140 kD gp is a plasma membrane glycoprotein which closely interacts with the detergent-resistant cytoskeleton of cultured human fibroblast. Possible mechanisms of the association are discussed.
mesh_terms: D002462:Cell Membrane / Q000032:analysis*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003599:Cytoskeleton / Q000032:analysis* / Q000648:ultrastructure; D005347:Fibroblasts; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D017830:Octoxynol; D010447:Peptide Hydrolases / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D013388:Succinimides / Q000494:pharmacology; D014746:Vimentin
chemical_list: D006023:Glycoproteins; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D013388:Succinimides; D014746:Vimentin; D011092:Polyethylene Glycols; D017830:Octoxynol; D010447:Peptide Hydrolases; C011240:dithiobis(succinimidylpropionate)