Lymphokine purification by reversed-phase high-performance liquid chromatography.
Reversed-phase high-performance liquid chromatography has been used to purify to homogeneity two different lymphokines. Human IL-2 was purified on a C8 reversed-phase column in pyridine-acetate-propanol followed by chromatography on a C18 reversed-phase column in trifluoroacetic acid-acetonitrile. Protein sequence analysis of in situ-generated cyanogen bromide peptides obtained from this preparation established the homogeneity of this material and confirmed the amino acid sequence predicted from the published DNA sequence. Murine CSF-2 alpha was purified on a C18 reversed-phase column in trifluoroacetic acid-acetonitrile followed by chromatography on the same column in pyridine-acetate-propanol. The final preparation yielded a single band on a sodium dodecyl sulfate-polyacrylamide gel with a molecular weight of 24,500.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification; D008222:Lymphokines / Q000302:isolation & purification*; D051379:Mice; D013601:T-Lymphocytes / Q000378:metabolism
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D008222:Lymphokines