title: Acquisitive evolution of ribitol dehydrogenase in Klebsiella pneumoniae.
abstract: Selection in continuous culture of Klebsiella pneumoniae mutants that have gained the ability to utilize xylitol while also retaining regulatory control over ribitol utilization was achieved with a dual-substrate regime. Initial steady-state cultures of wild-type organisms were maintained with 0.005% (0.329 mM) ribitol. Mutants of various types proliferated when the composition of the limiting medium was changed to 0.005% ribitol plus 0.250% (16.43 mM) xylitol.
mesh_terms: D003470:Culture Media; D004790:Enzyme Induction; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D009154:Mutation; D012255:Ribitol / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000096:biosynthesis*; D013402:Sugar Alcohols / Q000378:metabolism*; D014993:Xylitol / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013402:Sugar Alcohols; D012255:Ribitol; D013401:Sugar Alcohol Dehydrogenases; C022529:ribitol 2-dehydrogenase; D014993:Xylitol