title: [Participation of plasmid F in the replication of the chromosome of an Hfr strain of Escherichia coli K-12].
abstract: In the region of plasmid F DNA with coordinates 52,2-55,8 kb, the chr ("chromosome replication") locus has been revealed. A failure in the functioning of this locus in the integrated plasmid, which leads to a temperature-sensitive disturbance in chromosome replication of the Hfr strain and to the changes in its sensitivity to some membranotropic agents. Integration of an F segment containing the chr+ allele into the chromosome of an F-like derivative of such Hfr strain (retaining a mutant part of the F DNA), results in formation of temperature-resistant clones. In these clones, chromosomal replication is controlled by the plasmid replicon at the elevated temperature. It has been concluded that the F plasmid can control chromosome replication of the dna+ HfrC strain of Escherichia coli K-12 and that the product of the chr gene is a membrane protein involved in chromosomal replication.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D010957:Plasmids; D011995:Recombination, Genetic; D013696:Temperature
chemical_list: D004269:DNA, Bacterial
vernacular_title: Uchastie plazmidy F v replikatsii khromosomy Hfr shtamma Escherichia coli K-12.