Evaluation of the Dupont aca bromocresol purple albumin method.
The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon SMA 12/60. The BCP method was found to have more than adequate precision and its accuracy is as good as the EIA method. The BCP method will give results which are approximately 9 g/L lower than end point BCG methods. Problems have been identified with some of the commercial materials that could be used to standardize the BCP method, since different commercial preparations of human serum albumin do not react in identical fashion in the BCP and EIA methods. For this reason caution should be exercised when standardizing the BCP method.
D000818:Animals; D001322:Autoanalysis; D004396:Coloring Agents; D005069:Evaluation Studies as Topic; D006801:Humans; D007158:Immunologic Techniques; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis*
D004396:Coloring Agents; D012709:Serum Albumin