title: The effects of temperature on the activity of testicular steroidogenic enzymes.
abstract: Decreased sperm counts and impaired sperm motility are present in a substantial proportion of men with varicocele. Elevations in the temperature of the affected testis, and increased spermatic vein estradiol (E2) concentrations have been found in some of these patients. To investigate the possibility that increases in temperature lead to a pattern of testicular steroidogenesis that results in increased E2 synthesis, we have examined the effects of temperature changes on the activities of four important testicular steroidogenic enzymes. 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), 17-hydroxylase (17-OH), 17,20-desmolase (17,20-D) and aromatase activities were measured in the microsomal fraction of rat, pig and horse testes. Incubations were performed at 34 degrees C, 36 degrees C, and 38 degrees C. The activities of all 4 enzymes increased with each 2 degrees C temperature elevation in roughly proportional amounts. We conclude that minor elevations in incubation temperature are associated with increases in the in vitro activity of four key testicular steroidogenic enzymes.
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003349:Cortisone Reductase / Q000378:metabolism; D006736:Horses; D007985:Leydig Cells / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013552:Swine; D013696:Temperature; D013737:Testis / Q000201:enzymology*
chemical_list: D003349:Cortisone Reductase; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases