Polarization fluoroimmunoassay of 11-deoxycortisol in serum and saliva.
We developed polarization fluoroimmunoassays for 11-deoxycortisol in serum and saliva. To avoid interfering factors, the steroid was initially extracted from the biological fluids with dichloromethane. Assays could then be completed without any further separation procedures or need to correct for blank signals. The serum assay was suitable for following the response to the metyrapone test and results correlated acceptably with those by an established, specific, direct 125I-radioimmunoassay. The method is not sufficiently sensitive to detect 11-deoxycortisol in normal saliva, but greatly increased concentrations were found in post-metyrapone saliva and results agreed well with those by the radioimmunoassay as modified for salivary assay. Salivary 11-deoxycortisol assay would provide a convenient means of monitoring results of the metyrapone test.
D015065:17-Hydroxycorticosteroids / Q000032:analysis*; D003350:Cortodoxone / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D003429:Cross Reactions; D005454:Fluorescence Polarization; D005455:Fluorescent Antibody Technique; D006801:Humans; D008752:Methylene Chloride; D008797:Metyrapone / Q000378:metabolism*; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis* / Q000378:metabolism
D015065:17-Hydroxycorticosteroids; D008752:Methylene Chloride; D003350:Cortodoxone; D008797:Metyrapone