Effect of dexamethasone and combinations of hormones on 7-day chick embryo lung cells.
Seven-day chick embryo lung was dissociated into single cells and the capacity of these cells to incorporate 1-3H-glucosamine and 1-14C-glycine in a rotational-mediated aggregation system upon the addition of hormones was determined. Incorporation of 1-3H-glucosamine was stimulated 50% by 10(-8) M dexamethasone while 1-14C glycine uptake was not markedly influenced. Dexamethasone, 10(-8) M and 10(-6) M insulin gave rise to a 90% enhancement in 1-3H-glucosamine incorporation and a 20% increase in 14C-glycine uptake. When the aggregating cells were incubated with 10(-8) M dexamethasone and 10(-8) M 3,3',5-tri-iodo-thyronine, glucosamine incorporation was augmented by 40% and glycine by 25%. The combination, 10(-8) M 3,3',5-tri-iodo-thyronine, 10(-8) M dexamethasone and 10(-6) M insulin led to a 70% stimulation in glucosamine labelling and a 50% increase in 1-14C-glycine uptake. Very little change in the incorporation of the hexosamine and glycine into the soluble culture media was observed by adding either of the hormones; however, there appeared to be a slight increase in the incorporation of both tracers when the cells were incubated with the combination of dexamethasone and insulin.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003907:Dexamethasone / Q000494:pharmacology*; D005944:Glucosamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D014284:Triiodothyronine / Q000494:pharmacology*; D014285:Triiodothyronine, Reverse / Q000494:pharmacology*
D007328:Insulin; D014284:Triiodothyronine; D014285:Triiodothyronine, Reverse; D003907:Dexamethasone; D005944:Glucosamine; D005998:Glycine