A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus.
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically to produce a three base, 3' extension.
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D007700:Kinetics; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D013604:T-Phages
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C041749:endodeoxyribonuclease SfiI; D015252:Deoxyribonucleases, Type II Site-Specific