Comparison of a microneutralisation test with ELISA and precipitin tests for detection of antibodies to infectious bronchitis virus in chickens.
A microneutralisation test for infectious bronchitis virus using virus antigens available in Australia, cell culture medium containing low concentrations of serum and an elevated incubation temperature is described. The technique was economical on reagents and of comparable sensitivity to the enzyme-linked immunosorbent assay (ELISA). The value of the microneutralisation, ELISA and precipitin tests in assessing the serological response of a flock of commercial chickens to vaccine and natural virus challenge was determined.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001990:Bronchiolitis, Viral / Q000276:immunology / Q000662:veterinary*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests / Q000379:methods; D011201:Poultry Diseases / Q000276:immunology*; D011233:Precipitin Tests
D000914:Antibodies, Viral