Modulation of human interferon-alpha receptor expression by human interferon-gamma.
The human neuroblastoma cell line T98G is sensitive to the antiproliferative activity of both alpha- and gamma-interferons (IFNs). Specific binding of a homogeneous 125I-IFN-alpha 2 probe to high-affinity membrane receptors (apparent Kd = 6.2 X 10(-10) M) can be decreased by pretreatment of these cells with human IFN-gamma (HuIFN-gamma, derived by recombinant DNA techniques). The regulation of HuIFN-alpha 2 binding by such pretreatment is reflected by subsequent inhibition of both the antiviral and antiproliferative activities of HuIFN-alpha 2 on T98G cells. Thus, binding of HuIFN-gamma to its distinct cell-surface receptor can modify expression of the separate IFN-alpha receptor, presumably through an indirect effect on the apparent Kd of the IFN-alpha 2 receptor-ligand interaction.
D002051:Burkitt Lymphoma; D002460:Cell Line; D004686:Encephalomyocarditis virus / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism* / Q000633:toxicity; D007371:Interferon-gamma / Q000502:physiology*; D007700:Kinetics; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017471:Receptors, Interferon
D007370:Interferon Type I; D011956:Receptors, Cell Surface; D017471:Receptors, Interferon; D007371:Interferon-gamma