[Use of the toxin-tissue receptor reaction for detecting toxic substances of the causative agents of acute intestinal diseases].
The possibility of using erythrocytic ganglioside diagnostic reagents (EGDR) for the detection of V. cholerae, E. coli and S. typhimurium enterotoxins in the passive hemagglutination (PHA) test has been shown. Museum strains and cultures isolated from patients with acute intestinal diseases were tested for the presence of enterotoxins. Cell-free extracts were studied by biological methods and by serological titration in the PHA test with the use of EGDR. The diagnostic reagent was found to interact only with those enterotoxins whose specific receptors were gangliosides GM1.
D000208:Acute Disease; D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis*; D002772:Cholera Toxin / Q000032:analysis; D004768:Enterotoxins / Q000032:analysis*; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli; D005677:G(M1) Ganglioside; D005732:Gangliosides; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007410:Intestinal Diseases / Q000175:diagnosis*; D011817:Rabbits; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000187:drug effects*; D012475:Salmonella; D012760:Shigella; D013210:Staphylococcus; D014734:Vibrio cholerae
D004768:Enterotoxins; D005732:Gangliosides; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C023552:choleragen receptor; D005677:G(M1) Ganglioside; D002772:Cholera Toxin
Ispol'zovanie reaktsii toksin-tkanevyĭ retseptor dlia obnaruzheniia toksicheskikh substantsiĭ vozbuditeleĭ ostrykh kishechnykh zabolevaniĭ.