Use of an enzyme-linked immunosorbent assay for the detection of IgG antibodies to rinderpest virus in epidemiological surveys.
A comparison was made between an indirect enzyme-linked immunosorbent assay (ELISA) and the virus neutralisation test (VNT) for the detection of antibodies to rinderpest virus in field sera. The results did not agree for 6 per cent of the sera tested where 3 per cent of the samples gave ELISA positive/VNT negative and 3 per cent gave ELISA negative/VNT positive. The latter sera all had high levels of IgM antibody, which may indicate animals being at an early stage of infection or detection of a non-specific reaction. The ELISA results give a representative picture of the immune status for field surveys and a greater number of sera can be assayed with relative ease, compared to the traditional serum neutralisation test.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D009500:Neutralization Tests; D012301:Rinderpest / Q000276:immunology*; D012302:Rinderpest virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines