Enhanced transformation of human cells by UV-irradiated pSV2 plasmids.
Irradiating the plasmid pSV2-gpt with UV (254 nm) doses up to 200 J m-2 caused a dose-dependent increase in the yield of Gpt+ transformants when the plasmid was introduced into human cells by calcium phosphate coprecipitation. UV doses greater than 1 kJ m-2 were required to reduce the efficiency of transformation below that obtained with unirradiated DNA.
D001483:Base Sequence; D002460:Cell Line; D002678:Chimera; D004262:DNA Restriction Enzymes; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005354:Fibrosarcoma; D006801:Humans; D010430:Pentosyltransferases / Q000235:genetics; D010957:Plasmids / Q000528:radiation effects*; D014170:Transformation, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum
D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D004262:DNA Restriction Enzymes