title: Inactivation of potent pyrolysate mutagens by chlorinated tap water.
abstract: The potent mutagens 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2, 62450-07-1), 2-amino-6-methyldipyrido-[1,2-a:3', 2'-d]imidazole (Glu-P-1, 67730-11-4) and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ, 76180-96-6), isolated from pyrolysates of tryptophan and glutamic acid and from broiled sardines, respectively, were effectively degraded by chlorinated tap water with a concomitant loss of mutagenicity toward Salmonella typhimurium TA98 and TA100. The half-life of 10 microM IQ in the presence of 1.5 ppm of residual chlorine was less than 10 sec; those of Glu-P-1 and Trp-P-2 were 0.5-1 and 2-3 min, respectively. This means that a glass of chlorinated tap water (150 ml) containing 1.5 ppm of residual chlorine can break down about 200 micrograms of these pyrolysate mutagens within a couple of minutes.
mesh_terms: D002243:Carbolines / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D002713:Chlorine / Q000494:pharmacology*; D003296:Cooking; D006358:Hot Temperature; D007093:Imidazoles / Q000037:antagonists & inhibitors; D009152:Mutagenicity Tests; D009153:Mutagens / Q000037:antagonists & inhibitors*; D011804:Quinolines / Q000037:antagonists & inhibitors; D012486:Salmonella typhimurium / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet; D014881:Water Supply / Q000032:analysis
chemical_list: D002243:Carbolines; D007093:Imidazoles; D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D002713:Chlorine; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole