Enzyme immunoassay of human plasma fibronectin in malignant, collagen and liver diseases.
A competitive enzyme immunoassay for the measurement of human plasma fibronectin (PFN) was developed, using polystyrene beads as a solid phase and glucose oxidase as a marker enzyme. This method gave a reproducible standard curve in the range of 0.5-50 micrograms/ml. The mean PFN level in normal persons was 345 micrograms/ml (standard deviation 68.4 micrograms/ml, n = 30). The PFN levels in patients with malignant, collagen and liver diseases were also measured. The mean PFN level in patients with collagen disease was significantly higher than that in normal persons, whereas the mean PFN levels in patients with malignant and liver diseases were not significantly different.
D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D003095:Collagen Diseases / Q000097:blood*; D005260:Female; D005353:Fibronectins / Q000097:blood*; D005949:Glucose Oxidase / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques; D008107:Liver Diseases / Q000097:blood*; D008297:Male; D008722:Methods; D008875:Middle Aged; D012016:Reference Values
D001798:Blood Proteins; D005353:Fibronectins; D005949:Glucose Oxidase