[Propagation of Pixuna virus in rat embryo cultures].
The behaviour of cell cultures from 11 to 18 days-old Swiss albino mouse embryo infected with Pixuna virus was studied. Eleven day-old embryo cultures showed to be a permissive system supporting a productive and cytocidal infection. In contrast, the 13 to 18 day-old embryo cultures were also permissive and productive but no evidence of cytopathic effect was observed. D-glucosamine did not affect the infectivity titer of the Pixuna virus when 11 day-old embryo cultures were used. In these cultures the eclipse phase was observed between 15 and 90 min post-absorption (Figure 1).
D000818:Animals; D003588:Cytopathogenic Effect, Viral; D005944:Glucosamine / Q000494:pharmacology; D009924:Organ Culture Techniques; D051381:Rats / Q000196:embryology; D014035:Togaviridae / Q000187:drug effects / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication / Q000187:drug effects
D005944:Glucosamine
Propagación del virus Pixuna en cultivos de embrión de ratón.