title: The modulating influence of the fluidity of cell membrane on excision repair of DNA in UV-irradiated Escherichia coli.
abstract: When the extent of liquid holding recovery (LHR) was measured as a function of the temperature at the time of liquid holding and the Arrhenius plot was made, two distinctive phases for the LHR were demonstrated in UV-irradiated RecA- derivative of E. coli ole28E1, which are unable to synthesize and degrade unsaturated fatty acids. The inflection temperatures were 17-18 degrees C, 23-24 degrees C and 28-30 degrees C for linoleate-, oleate- and elaidate-grown cells, respectively. These temperatures well corresponded to the phase transition temperatures of the cell membrane supplemented with the fatty acid. It is therefore concluded that at least a component involved in in vivo excision repair in E. coli is associated with cell membrane.
mesh_terms: D002462:Cell Membrane / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000528:radiation effects*; D004926:Escherichia coli / Q000528:radiation effects*; D005227:Fatty Acids / Q000378:metabolism; D008560:Membrane Fluidity; D013816:Thermodynamics; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids