title: Regulatory citrate lyase mutants of Salmonella typhimurium.
abstract: Citrate lyase, the key enzyme of anaerobic citrate catabolism, could not be deleted from Salmonella typhimurium. The only class of mutants found had a mode of covalent regulation that strongly resembled the Escherichia coli system: citrate lyase was only active, i.e., acetylated, when a cosubstrate was present.
mesh_terms: D000107:Acetylation; D000693:Anaerobiosis; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003470:Culture Media; D004789:Enzyme Activation; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000235:genetics
chemical_list: D002951:Citrates; D003470:Culture Media; D009097:Multienzyme Complexes; D011773:Pyruvates; D019343:Citric Acid; D019289:Pyruvic Acid; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase