Production and purification of monoclonal T lymphocyte antigen binding molecules (TABM).
T lymphocyte antigen binding molecules are distinct from B cell immunoglobulins. These proteins were produced by an antigen-specific T cell hybrid as monoclonal products and these cells were successfully grown as an ascites. The T cell antigen binding proteins in ascites were harvested and purified in milligram amounts. The procedure we describe should provide a valuable source of large quantities of pure T lymphocyte antigen binding molecules. This method will allow for studies into their mechanisms of function in regulation of the immune response, biochemistry and molecular biology as B cell myelomas and monoclones provided for the study of immunoglobulins.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008222:Lymphokines / Q000096:biosynthesis* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*; D013945:Thymoma / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic