Reactivity of proteins in ribosomes from Saccharomyces cerevisiae with trypsin.
The susceptibility of 40S and 60S ribosomal subunits from Saccharomyces cerevisiae to digestion with varying concentrations of trypsin was studied by two-dimensional electrophoresis and quantitative measurements of the protein remaining with the ribosomal particles after trypsin treatment. Proteins from both subunits can be classified into three groups according to their rate of digestion by trypsin. These results are in good agreement with those obtained on the order of ribosomal assembly in vivo, i.e., proteins which are most susceptible to trypsin digestion have been shown to associate with the ribosomal particles at a relatively late stage of ribosome assembly.
D055598:Chemical Phenomena; D002621:Chemistry; D005656:Fungal Proteins; D010446:Peptide Fragments; D012269:Ribosomal Proteins; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D014357:Trypsin
D005656:Fungal Proteins; D010446:Peptide Fragments; D012269:Ribosomal Proteins; D014357:Trypsin