Multiple quantum two-dimensional 1H--15N nuclear magnetic resonance spectroscopy: chemical shift correlation maps for exchangeable imino protons of Escherichia coli tRNAMetf in water.
A procedure based on multiple quantum two-dimensional nuclear magnetic resonance spectroscopy is described for generation of 1H--15N chemical shift correlation maps. The method is used to obtain 15N chemical shifts for the exchangeable imino protons in 1H--15N units of site-specifically labeled Escherichia coli tRNAMetf in water. The high sensitivity and excellent chemical shift dispersion of the multiple quantum two-dimensional technique make it ideally suited for studying protonated nitrogens by NMR.
D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D011789:Quantum Theory; D012346:RNA, Transfer, Amino Acyl; D014867:Water
D012346:RNA, Transfer, Amino Acyl; C026464:tRNA(m)(Met), methionine-; D014867:Water