Enhancement of [3H]flunitrazepam binding by mianserin, in vivo.
Mianserin (5-20 mg/kg), like chlordiazepoxide (2.5-10 mg/kg), inhibits the shock-induced suppression of drinking (SSD) in rats. However, in contrast to chlordiazepoxide, the effect of mianserin is not blocked by the benzodiazepine antagonist, Ro 15-1788 (10 mg/kg). Although mianserin does not inhibit [3H]diazepam binding in vitro it has now been found to enhance [3H]flunitrazepam binding to mouse whole brain in vivo at 10-100 mg/kg p.o. These results suggest that mianserin does influence central benzodiazepine receptors, but the mechanism by which it does so differs from that of chlordiazepoxide.
D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism*; D001570:Benzodiazepinones / Q000494:pharmacology; D002707:Chlordiazepoxide / Q000494:pharmacology; D003292:Convulsants / Q000494:pharmacology; D003984:Dibenzazepines / Q000494:pharmacology*; D004327:Drinking Behavior / Q000187:drug effects*; D005442:Flumazenil; D005445:Flunitrazepam / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008803:Mianserin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D011963:Receptors, GABA-A
D001570:Benzodiazepinones; D003292:Convulsants; D003984:Dibenzazepines; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D008803:Mianserin; D005442:Flumazenil; D005445:Flunitrazepam; D002707:Chlordiazepoxide