An ELISA-based assay for quantitation of human interleukin 2.
An ELISA-based method for the quantitation of human interleukin 2 is described. A murine monoclonal antibody and rabbit polyclonal antibody, both of which recognize the various glycosylated forms of human IL2, were used in a sandwich technique together with alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin. The specificity of the reaction was dependent upon the monoclonal reagent. By generating a standard curve with a defined preparation of IL2, it was possible to quantitate accurately the concentration of the factor in crude lymphokine preparations and in serum samples from IL2-treated patients. Substances in human serum and mitogens such as phytohemagglutinin and phorbol myristic acetate did not interfere in the measurement. This assay provides a rapid, automated alternative to the biological assay generally used to quantitate IL2.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis* / Q000494:pharmacology; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D007376:Interleukin-2