Stimulatory protein kinase modulator, the cation-binding protein and megamodulin. 3. Isolation from baker's yeast.
Three peak-activity fractions of megamodulin were noted in Sephadex G-100 filtration of its crude preparation from baker's yeast (Saccharomyces cerevisiae). Megamodulin thus prepared and its Mg2+-binding complex were shown not only to augment the activity of rabbit brain megamodulin-dependent protein kinase I (M-PK1) but also to interact with arginine-rich histone.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D004789:Enzyme Activation; D047908:Intracellular Signaling Peptides and Proteins; D008274:Magnesium / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D011494:Protein Kinases; D008274:Magnesium