Decreased micrococcal nuclease sensitivity of nuclei from nerve growth factor-treated PC12 cells.
Nuclei from nerve growth factor-treated PC12 cells are more resistant to digestion with micrococcal nuclease than are nuclei from control cells. The production of oligosomal fragments is decreased, as is the generation of Mg2+-soluble products. One interpretation of the data is that differentiation of these cells due to treatment with nerve growth factor involves a decrease in the total number of DNA sequences transcribed.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects*; D004247:DNA / Q000032:analysis; D004357:Drug Synergism; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K; D010450:Endopeptidases / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology*
D009414:Nerve Growth Factors; D004247:DNA; D008836:Micrococcal Nuclease; D010450:Endopeptidases; D019286:Endopeptidase K; D008274:Magnesium