The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques.
A monoclonal mouse antibody to human thyroglobulin was used in a modified biotin-peroxidase-complex method to demonstrate thyroglobulin in different tissues and tumors of the thyroid gland. Mouse ascites fluid containing monoclonal antibodies could be diluted up to 1:1,600 for detection of thyroglobulin, whereas no staining was observed in a medullary carcinoma. Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies against thyroglobulin are suitable in the immunohistochemical localisation of thyroid hormones.
D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002291:Carcinoma, Papillary / Q000276:immunology; D006043:Goiter, Endemic / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D013954:Thyroglobulin / Q000276:immunology*; D013964:Thyroid Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D013954:Thyroglobulin