Comparative study of alpha-glucosidase activities in blood and seminal plasma of rams.
1. This study was undertaken to characterize the alpha-glucosidase present in blood and seminal plasma of two strains of pure-bred rams which are known as seasonal breeders. 2. pH profiles and activity levels were investigated in blood and seminal plasma using a sensitive spectrophotometric assay with para-nitrophenyl-alpha-D-glucoside as substrate. 3. According to their pH optimum, blood plasma and seminal plasma alpha-glucosidases were typically neutral and acid enzymes and significant differences were recorded in their physico-chemical properties, establishing the tissue specificity of the enzyme. 4. Notwithstanding the tissue under study, the nature of the alpha-glucosidase activity was similar in both strains of pure-bred rams.
D000818:Animals; D005959:Glucosidases / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D012661:Semen / Q000201:enzymology*; D012756:Sheep / Q000378:metabolism*; D013696:Temperature; D000520:alpha-Glucosidases / Q000097:blood / Q000378:metabolism*
D065089:Glycoside Hydrolase Inhibitors; D005959:Glucosidases; D000520:alpha-Glucosidases