Monoclonal antibodies against BLV-transformed sheep cells. Brief report.
Hybrid cell lines were prepared by the fusion of BALB/c myeloma, P3X63Ag 8.653 cells with the spleen cells of BALB/c mice that were immunized with SF-28 cells, sheep fibroblasts transformed with BLV in vitro. Five clones of hybrid progeny produced cytotoxic IgG1 antibodies against SF-28 cells. The tested monoclonal antibodies showed cytotoxic activity against 4 out of 6 BLV-transformed sheep fibroblasts as well as SF-28 cells but failed to react with other cells, including BLV-producing FLK cells, bovine lymphosarcomas and normal sheep cells. These monoclonal antibodies showed suppressive effect on colony formation of SF-28 cells in soft agar culture.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D006825:Hybridomas / Q000276:immunology; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D012190:Retroviridae / Q000235:genetics*; D012756:Sheep
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral