The kinetics and mechanism of pig-liver aldehyde reductase. Comparative studies with pyridine-3-aldehyde and p-carboxybenzaldehyde.
Initial-rate measurements were made of the reduction of pyridine-3-aldehyde and p-carboxybenzaldehyde by NADPH catalyzed by pig liver aldehyde reductase I. The initial velocity analysis and product inhibition data suggest that aldehyde reductase I obeys a compulsory-order mechanism with pyridine-3-aldehyde as substrate but follows a partially random-order pathway with p-carboxybenzaldehyde. The partially random-order pathway would be operative only at high concentrations of p-carboxybenzaldehyde. In both cases, aldehydes and the corresponding alcohol substrates inhibit the enzyme at high concentration. Abortive ternary complexes are shown to be formed with pyridine-3-aldehyde and with p-carboxybenzaldehyde. Dissociation of the coenzyme from the abortive ternary complex seems only to be observed with p-carboxybenzaldehyde. This study suggests overall that an enzyme kinetic mechanism may be different, depending on whether specific interactions can occur between certain amino acid residue(s) of the protein active site and substrates. Finally, the mechanism of the inhibition of pyridine-3-aldehyde reduction by diacid derivatives is discussed.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology*; D055598:Chemical Phenomena; D002621:Chemistry; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D013379:Substrate Specificity; D013552:Swine
D000447:Aldehydes; D001547:Benzaldehydes; C033525:4-carboxybenzaladehyde; C014785:3-pyridinaldehyde; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+)