title: The use of metallocenes for protein modification. Formation of tetramethylcyclobutadiene nickel alcohol dehydrogenase.
abstract: Tetramethylcyclobutadiene nickel dichloride is a dark red, half-sandwich nickel metallocene with both aqueous and organic solubility. It reacts extensively with horse liver alcohol dehydrogenase (EC 1.1.1.1), forming an orange, soluble adduct that is stable at least to moderate dialysis and essentially devoid of native zinc and also enzymatic activity. This modified enzyme contains nearly 8 mol nickel per mol and possesses a positive Cotton effect. Under the same conditions, nickel chloride has no effect on the enzyme. This illustrates the potential for half-sandwich metallocenes to provide unique reactivity and spectral characteristics for protein modification.
mesh_terms: D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002942:Circular Dichroism; D006736:Horses; D007202:Indicators and Reagents; D008099:Liver / Q000201:enzymology; D009532:Nickel / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D007202:Indicators and Reagents; D009942:Organometallic Compounds; C038880:tetramethylcyclobutadiene nickel; D009532:Nickel; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase