[Dark and clear Purkinje's cells in the cerebellum during the postresuscitation period].
Interference microscopy was used to determine dry mass of the nucleus and cytoplasm of light, dark and morphologically changed Purkinje's cells (PC) of the cerebellum of dogs which had suffered clinical death of varying etiology as compared with intact animals. Two weeks after the 12-minute circulatory arrest the area of the nucleus of both light and dark PC sharply increased. Dry mass of the nucleus of dark PC and of the cytoplasm of both PC types also became greater. Two weeks following the 10-minute clinical death because of acute hemorrhage dry mass of the nucleus and cytoplasm of dark PC increased whereas in light PC it was unchanged as compared to the control level. In morphologically changed PC of dogs belonging to the both experimental groups, dry mass of the cytoplasm was essentially higher than in intact animals. It might be assumed that an increase in dry mass of the nucleus and cytoplasm has bearing on intracellular reparative regeneration. Taking into consideration that in intact dogs, dry mass of the cytoplasm and the concentration of dense substances in the nucleus of dark and light PC are similar, it should be suggested that light and dark PC differ in their roles in the maintenance of the population homeostasis rather than in the metabolic rate.
D000818:Animals; D003584:Cytological Techniques; D003645:Death, Sudden / Q000473:pathology*; D004285:Dogs; D011689:Purkinje Cells / Q000473:pathology*; D012151:Resuscitation; D013997:Time Factors
Temnye i svetlye kletki Purkin'e mozzhechka v postreanimatsionnom periode.