title: [Interaction between immobilized plastocyanin and cytochrome f and the photosystem I reaction center in a reconstituted system].
abstract: The effects of redox conversions of plastocyanin copper chromophore on the formation of plastocyanin complexes with cytochrome f and the reaction center of photosystem I from pea chloroplasts were studied. In order to investigate the complex formation plastocyanin and cytochrome f were immobilized on Sephadex G-200. The cytochrome f and reaction center assembly takes place on the immobilized plastocyanin, which is necessary for cytochrome f photooxidation. It was found that in a reconstituted system the reduced plastocyanin forms more stable complexes with the proteins than the oxidized one, which is due to its lower pI value.
mesh_terms: D002736:Chloroplasts / Q000378:metabolism*; D003580:Cytochromes / Q000378:metabolism*; D045348:Cytochromes f; D007700:Kinetics; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D010970:Plastocyanin / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D003580:Cytochromes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D010970:Plastocyanin; D045348:Cytochromes f
vernacular_title: Issledovanie vzaimodeĭstviia immobilizovannogo plastotsianina s tsitokhromom f i reaktsionnym tsentrom fotosistemy I v rekonstruirovannoĭ sisteme.