Testis cell-specific proteins in testosterone-treated rat ovaries.
Two-dimensional gel electrophoresis of rat gonads in various developmental stages (embryonic days 16, 17, 21; postnatal day 5) reveals an increasing number of polypeptide spots; 0.6%-2% of the polypeptides are gonad-specific and increase from embryonic day 16 onwards. Exposure of newborn rat ovaries to testosterone for 5 days results in the appearance of eight polypeptides. These polypeptides are absent in control ovaries but present in the testis from embryonic day 16 or 17 onwards. Three do not appear in the ovary at any developmental stage. These findings indicate that testosterone plays a physiologic role in normal testicular differentiation. After long-term testosterone treatment, ovaries are depleted of germ cells. This might explain the degeneration of oocytes in the abnormal environment of a testis (e.g., in XX true hermaphrodites).
D000818:Animals; D000831:Animals, Newborn; D005260:Female; D008297:Male; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012733:Sex Differentiation / Q000187:drug effects; D012741:Sexual Maturation / Q000187:drug effects; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology*
D011506:Proteins; D013739:Testosterone