Studies on Paul-Bunnell (P-B) antigen-antibody system. V. Immunoglobulin classes of P-B antibodies.
By means of enzyme immunoassay (EIA) with purified Paul-Bunnell (P-B) antigen of bovine erythrocytes and sera of infectious mononucleosis, P-B antibodies of IgG (92%), IgM (94%), IgA (77%), and IgE (64%) classes were demonstrated. Absorption and inhibition studies ascertained the P-B specificity of the antibodies of all four classes. Absorption of selected IM sera with sheep erythrocytes revealed the presence of both BS and B antibodies of P-B specificity in these sera. In three IM patients whose sequential serum samples were studied, P-B antibodies of all four classes appeared and reached their peaks within a few weeks after the onset of the disease, persisted for a month, and sharply declined thereafter.
D000906:Antibodies / Q000145:classification*; D000910:Antibodies, Heterophile / Q000276:immunology*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000145:classification; D007073:Immunoglobulin E / Q000145:classification; D007074:Immunoglobulin G / Q000145:classification; D007075:Immunoglobulin M / Q000145:classification; D007244:Infectious Mononucleosis / Q000276:immunology
D000906:Antibodies; D000910:Antibodies, Heterophile; D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E