Immunocytochemistry of serotonin-containing nerves in the human gut.
An immunocytochemical technique, using L-tryptophan enhancement and paraformaldehyde fixation, has been successfully applied for the demonstration of serotonin-containing neurons in the human enteric nervous system. Throughout the intestine, serotonin-immunoreactive nerves were mainly detected in the myenteric plexus and, to a smaller extent, in the submucous plexus, while hardly any nerve fibres were seen in the mucosa. This approach provides a useful tool for the investigation of neuropathological conditions of the gut.
D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006801:Humans; D007420:Intestine, Large / Q000294:innervation; D007421:Intestine, Small / Q000294:innervation; D007422:Intestines / Q000294:innervation*; D009197:Myenteric Plexus / Q000032:analysis*; D009412:Nerve Fibers / Q000032:analysis*; D012701:Serotonin / Q000032:analysis*
D012701:Serotonin