[Exotoxin A production during Pseudomonas aeruginosa PA-7 cultivation in Martin's broth].
The activity of exotoxin A in culture filtrates prepared from cultures obtained by growing P. aeruginosa strains PA-7 and PA-103 in Martin's broth containing iron at a concentration of 0.08 microgram/ml, 0,05 M sodium glutamate and 1% of glycerin has been shown to be 1.5 times higher than that in filtrates prepared from cultures obtained by growing the above strains in a medium containing soybean tryptic digestion (USA). The optimun conditions for the production of exotoxin A by these strains are achieved during their cultivation in a fermenter at a temperature of 32 degrees C for 18 hours with simultaneous stirring (800 r. p. m.) and oxygenation (450 m3/h). Under these conditions the biological activity of the filtrates is 200 LD50/ml, their ADP-ribosyltransferase activity is 9500 c. p. m. and a sharply defined precipitation line appears in the double diffusion test in gel with monospecific antiserum to purified toxin, used in a dilution of 1:8.
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D001431:Bacteriological Techniques; D003470:Culture Media / Q000378:metabolism*; D005098:Exotoxins / Q000032:analysis / Q000096:biosynthesis*; D005779:Immunodiffusion; D009713:Nucleotidyltransferases / Q000032:analysis; D011065:Poly(ADP-ribose) Polymerases; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D003470:Culture Media; D005098:Exotoxins; D009713:Nucleotidyltransferases; D011065:Poly(ADP-ribose) Polymerases; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
Produktsiia ékzotoksina A pri kul'tivirovaniia Pseudomonas aeruginosa PA-7 na bul'one Martena.