Mast cell products stimulate collagenase and prostaglandin E production by cultures of adherent rheumatoid synovial cells.
Mast cells were purified from histologically-confirmed dog mastocytomas and extracted for whole mast cell products (MCP). When added to cultures of human adherent rheumatoid synovial cells MCP induced a 50-400 fold increase in prostaglandin E synthesis and a 10-50 fold stimulation of collagenase production. The mast cell stimulatory factor has not been identified and was not due to histamine, heparin or prostaglandin E. These results indicate a novel way in which mast cells might interact with synovial cells to promote the production of inflammatory mediators and proteolytic enzymes which might contribute to connective tissue degradation.
D000818:Animals; D002448:Cell Adhesion; D002457:Cell Extracts / Q000494:pharmacology*; D002478:Cells, Cultured; D004285:Dogs; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D003012:Microbial Collagenase / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis*; D012216:Rheumatic Diseases / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D011458:Prostaglandins E; D014020:Tissue Extracts; D003012:Microbial Collagenase