Human skin storage techniques: a study utilizing a nude mouse recipient.
The nude mouse model is an excellent technique for studying the viability of stored human skin. In this study Roswell Park Memorial Institute (RPMI) tissue culture medium provided reliable storage of viable human skin for 20 days. Antibiotic concentration plays a role in stored skin viability with low concentrations of penicillin and streptomycin providing adequate bacterial control and improved viability when compared to identical media with high concentrations of the same antibiotics. Addition of fetal calf serum to the storage medium did not improve human skin viability in this experiment.
D000818:Animals; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude / Q000502:physiology*; D016038:Skin Transplantation; D014021:Tissue Preservation / Q000379:methods*