Isolation and characterization of monoclonal antibodies to Shiga toxin.
Hybridoma cell lines which produce monoclonal antibodies to Shiga toxin from Shigella dysenteriae 1 were prepared. The monoclonal antibodies were all of the immunoglobulin G1 isotype and differed in their ability to neutralize cytotoxicity and to bind to Shiga toxin in a solid-phase radioimmunoassay. When used for immunoblot analysis, these antibodies were able to identify specifically both nicked and unnicked Shiga toxin in crude lysates of S. dysenteriae.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D001427:Bacterial Toxins / Q000032:analysis / Q000276:immunology*; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D011863:Radioimmunoassay; D022603:Shiga Toxins; D012762:Shigella dysenteriae / Q000032:analysis*
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D007074:Immunoglobulin G; D022603:Shiga Toxins