Improved purification and characterization of the OXA-2 beta-lactamase.
An improved and scaled-up procedure has been developed for purifying the OXA-2 plasmid-mediated beta-lactamase. This has enabled us to improve the characterization of this enzyme, including a revised determination of its amino acid composition and the sequence of the N-terminal region of the protein.
D000596:Amino Acids / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D003001:Cloning, Molecular; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics / Q000302:isolation & purification*
D000596:Amino Acids; C039404:beta-lactamase OXA-2; D001618:beta-Lactamases