Partial characterisation of the high and low molecular weight forms of P388D1-derived interleukin 1.
The murine macrophage-derived cell line P388D1 secretes the lymphokine interleukin 1 (IL-1) when stimulated by a variety of agents. When stimulated by bacterial lipopolysaccharide (LPS) the cells release IL-1 in both high and low molecular weight (m.w.) forms. The proportion of high m.w. IL-1 is reduced when IL-1-containing supernatants are concentrated by ammonium sulfate precipitation subsequent to hollow-fiber filtration. The high m.w. form can be converted to the low m.w. form by proteolysis, reduction and alkylation, or chromatography in a dissociating solvent. The low m.w. form remains as such, even when reconcentrated in fetal calf serum-containing medium. The high m.w. form thus likely consists of a complex between low m.w. IL-1 and another protein secreted by the P388D1 cell line.
D000478:Alkylation; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D005590:Fractional Precipitation; D007375:Interleukin-1 / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D010084:Oxidation-Reduction; D014357:Trypsin
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014357:Trypsin