Monoclonal antibody to an integral membrane protein, the lactose permease.
A monoclonal IgG antibody directed against the lactose permease was produced from animals inoculated with membranes of a lac Y plasmid strain. The appropriate antibody was selected by a series of ELISA assays in which membranes, purified permease, or a lac Y-Z chimeric protein was the immobilized antigen. The antibody recognizes a portion of the permease exposed on the surface of membrane vesicles but does not inhibit lactose transport.
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000276:immunology*; D026901:Membrane Transport Proteins / Q000276:immunology*; D009004:Monosaccharide Transport Proteins; D013499:Surface Properties; D027981:Symporters
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; C050221:LacY protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease