title: Different potencies of biosynthetic human and purified porcine insulin.
abstract: The glucose clamp technique was used to compare the biological activity of purified porcine insulin and Biosynthetic Human Insulin (BHI). An intravenous bolus of 0.1 U/kg BW was injected in eight male volunteers, and the glucose was clamped at baseline values (euglycemic clamp). Serum insulin, serum C-peptide and plasma glucose did not differ between porcine and human insulin. The insulin induced glucose consumption differed significantly (p less than 0.007) between purified porcine insulin (50.5 +/- 5.2 [SEM] g/2h) and Biosynthetic Human Insulin (63.5 +/- 4.5 g/2h). Purified porcine insulin induced a hormonal response with significantly (p less than 0.05) elevated concentrations of serum growth hormone (12.1 +/- 0.25 ng/ml) and serum cortisol (161.4 +/- 28.6 ng/ml), which were not observed following Biosynthetic Human Insulin (serum growth hormone: 2.6 +/- 0.2 ng/ml; serum cortisol: 117.3 +/- 14.8 ng/ml). The data confirm earlier results indicating hormonal and metabolic differences between human and porcine insulin.
mesh_terms: D000328:Adult; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000097:blood; D013006:Growth Hormone / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D013045:Species Specificity; D013552:Swine
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D007328:Insulin; D013006:Growth Hormone; D006854:Hydrocortisone