Reconstitution of the GalP galactose transport activity of Escherichia coli into liposomes made from soybean phospholipids.
Galactose transport activity from Escherichia coli was solubilized with octyl glucoside, and reconstituted into liposomes made from soybean or E. coli lipid. Galactose counterflow in the proteoliposomes was inhibited by glucose, talose, 2-deoxygalactose and 6-deoxygalactose, confirming that it was due to GalP and not one of the other E. coli galactose transport systems.
D001693:Biological Transport, Active; D004926:Escherichia coli / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005960:Glucosides; D007700:Kinetics; D008081:Liposomes; D012995:Solubility; D013025:Glycine max
D005960:Glucosides; D008081:Liposomes; C018619:octyl-beta-D-glucoside; D005690:Galactose