High-performance liquid chromatographic analysis of tiaprofenic acid and its metabolites in plasma and urine by direct injection.
A rapid, convenient, sensitive and selective reversed-phase high-performance liquid chromatographic method was developed to measure tiaprofenic acid, its reduced and oxidized metabolites and their conjugates in biological fluids. The method involved direct injections of plasma and urine samples into the chromatograph before and after alkaline hydrolysis of the conjugates. Concentrations as low as 0.5 micrograms/ml of the drug in plasma and urine were quantifiable. The method was suitable for analysis of tiaprofenic acid and its metabolites in biological fluids after administration of therapeutic doses. Several other non-steroidal anti-inflammatory drugs which were applied to the system did not interfere with the assay.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000378:metabolism; D001826:Body Fluids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005260:Female; D006207:Half-Life; D006801:Humans; D008297:Male; D011422:Propionates / Q000032:analysis* / Q000097:blood / Q000627:therapeutic use / Q000652:urine
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011422:Propionates; C021270:tiaprofenic acid