Preparation of biologically active components of Mycobacterium bovis, using Triton X-100 or potassium chloride.
Methods of producing antigen extracts of Mycobacterium bovis are described. Extraction of M bovis with a chaotropic salt, potassium chloride, or a nonionic detergent, Triton X-100, before autoclaving resulted in the production of soluble M bovis components. These antigens induced delayed-type hypersensitivity responses when injected intradermally in M bovis-sensitized guinea pigs. A comparison of the diameters of the hypersensitivity responses (on the basis of equal protein concentrations in the inocula) revealed that the potassium chloride extract and the Triton extract of M bovis induced similar responses compared to the response induced with a purified protein derivative of M bovis. These findings indicate alternative methods may be used for the production of biologically active extracts of M bovis.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008722:Methods; D009163:Mycobacterium bovis / Q000276:immunology*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011189:Potassium Chloride
D000942:Antigens, Bacterial; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D017830:Octoxynol