Measurement of insulin binding by erythrocyte ghosts. A method that allows storage without loss of binding characteristics.
The insulin binding of red cell ghosts was studied and found to be similar to insulin binding of the original erythrocyte. Red cell ghosts were stable on storage, and serial samples can be measured in one assay, thus eliminating the problem of interassay variation. Erythrocyte ghosts may be preferable to whole red cells for long-term insulin receptor studies.
D002118:Calcium / Q000494:pharmacology; D003920:Diabetes Mellitus / Q000378:metabolism*; D004906:Erythrocyte Count; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D011309:Preservation, Biological; D011972:Receptor, Insulin / Q000378:metabolism*; D013048:Specimen Handling; D013997:Time Factors
D007328:Insulin; D011972:Receptor, Insulin; D002118:Calcium