Topological arrangement of two transfer RNAs on the ribosome. Fluorescence energy transfer measurements between A and P site-bound tRNAphe.
The relative arrangement of two tRNAPhe molecules bound to the A and P sites of poly(U)-programmed Escherichia coli ribosomes was determined from the spatial separation of various parts of the two molecules. Intermolecular distances were calculated from the fluorescence energy transfer between fluorophores in the anticodon and D loops of yeast tRNAPhe. The energy donors were the natural fluorescent base wybutine in the anticodon loop or proflavine in both anticodon (position 37) and D loops (positions 16 and 17). The corresponding energy acceptors were proflavine or ethidium, respectively, at the same positions. Four distances were measured: anticodon loop-anticodon loop, 24(+/- 4) A; anticodon loop (A site)-D loop (P site), 46(+/- 12) A: anticodon loop (P site)-D loop (A site), 38(+/- 10) A: D loop-D loop, 35(+/- 9) A. Assuming that both tRNAs adopt the conformation present in the crystal and that the CCA ends are close to each other, the results are consistent with the two anticodons being bound to contiguous codons and suggest an asymmetric arrangement in which the planes of the two L-shaped molecules enclose an angle of 60 degrees +/- 30 degrees.
D000926:Anticodon; D004735:Energy Transfer; D004926:Escherichia coli; D006147:Guanine / Q000031:analogs & derivatives; D011370:Proflavine; D014176:Protein Biosynthesis; D012343:RNA, Transfer; D012346:RNA, Transfer, Amino Acyl; D012270:Ribosomes / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; C012099:wye; D006147:Guanine; D012343:RNA, Transfer; D011370:Proflavine