title: Estimation of airborne rat-derived antigens by ELISA.
abstract: Double antibody sandwich ELISA procedures have been devised to estimate the concentrations of airborne rat urinary and epidermal antigens. Both assays were capable of detecting at least 3 ng/ml of antigen protein. Unknown concentrations were estimated in terms of the standard, laboratory-prepared material, as calculated from the linear relationship between absorbance and log concentration. The coefficient of variation of a single assay was 12%, the methods have been applied to extracts of the filter material from static and personal samplers in an animal room.
mesh_terms: D000485:Allergens / Q000032:analysis*; D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats / Q000276:immunology*; D011919:Rats, Inbred Strains; D012867:Skin / Q000276:immunology
chemical_list: D000485:Allergens; D000941:Antigens