title: Intracellular activation of albomycin in Escherichia coli and Salmonella typhimurium.
abstract: The antibiotic albomycin is actively taken up by Escherichia coli via the transport system for the structurally similar iron complex ferrichrome. Albomycin is cleaved, and the antibiotically active moiety is released into the cytoplasm, whereas the iron carrier moiety appears in the medium. Besides transport-negative mutants, additional albomycin-resistant mutants were isolated. The mutations were mapped outside the transport genes close to the pyrD gene at 21 min. The mutants were devoid of peptidase N activity. The molecular weight, sensitivity to inhibitors, and cytoplasmic location of the enzyme hydrolyzing albomycin in vitro corresponded to the known properties of peptidase N. The aminoacyl thioribosyl pyrimidine moiety of albomycin apparently has to be cleaved off the iron chelate transport vehicle to inhibit growth. Peptidase N is the major hydrolyzing enzyme. In Salmonella typhimurium peptidase N and peptidase A were equally active in hydrolyzing and activating albomycin.
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases; D001711:Biotransformation; D010450:Endopeptidases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005291:Ferrichrome / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D005291:Ferrichrome; C015657:albomycin; D010450:Endopeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases; C034113:peptidase N; C022325:aspartic proteinase A; D016282:Aspartic Acid Endopeptidases