A simple immunoenzyme batch staining method for the enumeration of peripheral human T lymphocyte subsets.
A simple batch method was developed for enumeration of peripheral human T lymphocyte subsets by immunoenzyme staining of stored prefixed preparations of peripheral mononuclear cells on multiple well microscope slides. By this method the monoclonal antibodies Leu 1, Leu 2a and Leu 3a stained the same number of cells as by indirect immunofluorescence on suspended cells.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D016030:Kidney Transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007202:Indicators and Reagents