title: Induction of expression of the rat G5 nervous system antigen occurs postnatally.
abstract: G5 is a cell membrane component found in high levels in the adult rat central nervous system and in low levels on some cells of the rat immune system. Binding assays with adult brain particulate protein preparations and monoclonal antibody to G5 (G5-IgG) gave highest activity in cerebral cortex and lowest in the white matter rich regions pons and spinal cord. Three peripheral nervous system components had no G5 activity. Analysis of G5 content in particulate protein preparations from whole rat brain of ages embryonic day 15 to adult indicates that G5 is present in negligible amounts in the newborn rat. It increases in both specific and total activity to reach adult levels by postnatal day 30. A similar induction curve was observed with cerebellum samples. In adult cerebellum, high levels of G5 were found in the molecular layer using both autoradiographic and immunofluorescence techniques. White matter had essentially no activity. The density and uniformity of reaction product in these techniques suggest that G5 is present on a major cellular constituent of the molecular layer. Pharmacologic experiments indicate G5 is not detectable on climbing fibers or adrenergic fibers. Cerebellar samples from juvenile rats also had predominant G5 activity in the forming molecular layer.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D002454:Cell Differentiation; D002531:Cerebellum / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000276:immunology; D011149:Pons / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000276:immunology; D013570:Synaptic Membranes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins; D007074:Immunoglobulin G