Differential expression of a lamininlike substance by high- and low-metastatic tumor cells.
High-metastatic murine fibrosarcoma cells readily attached to Type IV (basement membrane) collagen, whereas low-metastatic cells isolated from the same tumor did not. The addition of laminin--a glycoprotein that facilitates the adherence of epithelial cells to their basement membranes--enhanced the attachment of the low-metastatic cells, but not the high-metastatic cells. Using anti-laminin antibodies and a laminin-binding lectin as probes, the authors were able to identify by immunofluorescence a moiety associated with the high-metastatic cells, but not the low-metastatic cells, which cross-reacted with murine laminin purified from the EHS sarcoma. When extracts from the high-metastatic cells were separated by affinity chromatography, with the laminin-binding lectin as the affinity substrate, a substance was isolated that had an apparent molecular weight of 56,000 daltons. The affinity-purified material reacted strongly with anti-laminin antibodies by enzyme-linked immunosorbent assay.
D000818:Animals; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D003094:Collagen; D003602:Cytotoxicity, Immunologic; D005354:Fibrosarcoma / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D007797:Laminin; D037102:Lectins / Q000032:analysis; D051379:Mice; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000032:analysis / Q000276:immunology
D006023:Glycoproteins; D007797:Laminin; D037102:Lectins; D009363:Neoplasm Proteins; D003094:Collagen