Rapid proteolysis of unstable globins in human bone marrow.
To determine whether human red cells contain a proteolytic system capable of rapidly degrading unstable proteins, the fate of pulse-labelled puromycyl polypeptides was investigated. In erythroid bone marrow cells these unstable polypeptides were degraded to TCA-soluble fragments with a mean half-life of 4 . 5 min. However, in peripheral blood reticulocytes no proteolysis was observed, indicating that this activity declines as the red cell matures.
D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004588:Electrophoresis, Cellulose Acetate; D005914:Globins / Q000378:metabolism*; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011691:Puromycin / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism
D010455:Peptides; D011691:Puromycin; D005914:Globins; D010447:Peptide Hydrolases