Expansion of murine cytotoxic precursors in vitro and in vivo by purified interleukin-2.
Precursors of antigen-specific cytotoxic T cells from mice pretreated with immunosuppressive doses of cyclophosphamide can be expanded in vitro and in vivo by low doses of human interleukin-2. The effector cells are Thy 1+ and are not elicited in the absence of antigen. High doses of human interleukin-2 in vitro can expand both normal and cyclophosphamide-resistant T cell precursors into effector cells capable of lysing both natural killer cell-sensitive and -insensitive target cells in the absence of antigen.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000276:immunology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology / Q000628:therapy; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*
D007376:Interleukin-2; D003520:Cyclophosphamide