[Detection of rubella-specific IgM by an immunoenzyme technic].
An enzyme-linked immunosorbent assay (ELISA) was compared to sucrose gradient centrifugation technique for rubella specific IgM detection of 114 sera. The 28 sera which were rubella specific IgM positive, were also positive by ELISA. Among the 86 sera which were rubella IgM negative by centrifugation, 6 were equivocal and 2 were rubella IgM positive by the ELISA test, without clinical or epidemiological connection. These false results make a necessity of confirming by an immunoglobulin separation technique the ELISA IgM positive sera.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M
Détection des IgM spécifiques de la rubéole par une technique immuno-enzymatique.