title: Differential protein synthesis in Candida albicans during blastospore formation at 24.5 degrees C and during germ tube formation at 37 degrees C.
abstract: To identify proteins synthesized during blastospore to germ tube transformation in Candida albicans, membrane and cytoplasmic protein fractions labelled with 14C were analysed by SDS-PAGE and autoradiography. Four cytoplasmic proteins were detected in pulse-labelled lysates prepared from cells forming blastospores and germ tubes at 37 degrees C, but not in pulse-labelled lysates prepared from cells forming blastospores at 24.5 degrees C. Three proteins detectable in 24.5 degrees C lysates were diminished in 37 degrees C lysates.
mesh_terms: D001345:Autoradiography; D002176:Candida albicans / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis*; D005775:Gastrula / Q000378:metabolism*; D008970:Molecular Weight; D013696:Temperature
chemical_list: D005656:Fungal Proteins