A new method for the preparation of anti-idiotypic antibodies against six different human cold agglutinins.
A method based on sequential immunoadsorption for the preparation of a highly specific antibody directed against the idiotypic determinant of human cold agglutinins (CA) is described. In the present study, human cold agglutinins (CA) were used as a model, but the method can easily be adapted for the preparation of anti-idiotypic antibodies against other monoclonal human immunoglobulins. Since regenerable immunoadsorbents were used, the isolation can be accomplished even with rather low amounts of the respective immunoglobulin.
D000373:Agglutinins / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D003450:Cryoglobulins; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007163:Immunosorbent Techniques; D011817:Rabbits
D000373:Agglutinins; D000888:Antibodies, Anti-Idiotypic; D003450:Cryoglobulins; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; C029443:cold agglutinins