[Induction of the replicative synthesis of DNA by SV40 virus T antigen introduced into cells using liposomes].
The role of virus SV40 T-antigen in the induction of cell DNA synthesis during its incorporation into cell liposomes was studied, using monolamellar liposomes obtained by phase reversal with incorporated highly purified T-antigen. Immunofluorescence studies revealed that T-antigen effectively penetrates inside the cells and after 10 hours is accumulated in the nuclei, where its level remains unchanged for 24 hours. Injections of purified T-antigen into the renal cells of serum-starved CV1 monkeys resulted in an almost 10-fold increase in the number of DNA-synthesizing cells 18 hours after the exposure. The same effect was observed during stimulation of a 10% serum culture. Removal of T-antigen from the preparation by specific immunoadsorption eliminated this effect. Centrifugation of cells grown in the presence of bromodeoxyuridine in a CsCl gradient was used to demonstrate the replicative type of cell DNA synthesis during T-antigen induction.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D007668:Kidney; D008081:Liposomes; D008940:Mitotic Index; D009500:Neutralization Tests; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014779:Virus Replication
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D008081:Liposomes
Induktsiia replikativnogo sinteza DNK T-antigenom virusa SV40, vvedennogo v kletki s pomoshch'iu liposom.