Nylon tube linked acetylcholine receptor: a tool for affinity isolations and immunosorption.
We have covalently coupled acetylcholine receptor and other proteins to the inner surface of nylon tubes and employ these affinity tubes in binding assays and for chromatographic purposes. Here we describe two applications: (i) The concentration determination of toxins and antibodies directed against the acetylcholine receptor, and (ii) the isolation and chromatography of specific immunoglobulins.
D000818:Animals; D004557:Electric Organ / Q000032:analysis; D007163:Immunosorbent Techniques; D009757:Nylons; D011485:Protein Binding; D011950:Receptors, Cholinergic / Q000302:isolation & purification*; D014101:Torpedo
D009757:Nylons; D011950:Receptors, Cholinergic