Influence of ionic strength on the antibody binding of MHC products.
Ionic strength has a strong influence on the binding of human Ia and HLA(A,B,C) molecules by the corresponding antibodies. A control system, BSA-anti-BSA, was influenced only minimally. The effect on the binding of anti-beta 2-microglobulin was very small when isolated chains were used, but more pronounced when using whole HLA(A,B,C) molecules. An inverse relationship exists between degree of inhibition and antibody concentration.
D000937:Antigen-Antibody Reactions; D006680:HLA Antigens / Q000276:immunology*; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D009994:Osmolar Concentration; D001613:beta 2-Microglobulin / Q000276:immunology
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D000949:Histocompatibility Antigens Class II; D001613:beta 2-Microglobulin