title: Concanavalin A inhibits nicotinic acetylcholine receptor function in cultured chick ciliary ganglion neurons.
abstract: The effects of various lectins and toxins on neuronal nicotinic acetylcholine receptor function have been studied in primary cultures of chick ciliary ganglion neurons. Neuronal response to acetylcholine receptor activation was measured by a cation flux method at 4 degrees C in a high potassium-low sodium medium designed to stabilize membrane potential near zero, with acetylcholine as the agonist and cesium-137 as the tracer ion. Exposure to 1 mM acetylcholine for 30 s produced a 5-10-fold stimulation of cesium-137 influx. Acetylcholine-stimulated influx was inhibited more than 95% by 10 microM D-tubocurarine, but was insensitive to both 1 microM tetrodotoxin and 1 microM alpha-bungarotoxin. Concanavalin A (50 micrograms/ml) inhibited agonist-induced ion flux by 80% at 4 degrees C. Succinyl-concanavalin A was ineffective at concentrations up to 250 micrograms/ml, and could not protect against the concanavalin A inhibition. However, inhibition by concanavalin A was eliminated by prior incubation of the lectin with 0.2 M alpha-methyl-D-mannoside and subsequent co-incubation with the sugar. Wheat germ agglutinin, lentil lectin, cholera toxin and tetanus toxin were without effect at either 4 degrees C or 37 degrees C. These results suggest a specific interaction between concanavalin A and neuronal nicotinic acetylcholine receptors.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002642:Chick Embryo; D002772:Cholera Toxin / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D046508:Culture Techniques; D005726:Ganglia, Parasympathetic / Q000187:drug effects*; D007473:Ion Channels / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009433:Neural Inhibition / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011978:Receptors, Nicotinic / Q000187:drug effects*; D013744:Tetanus Toxin / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D037102:Lectins; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D013744:Tetanus Toxin; D003208:Concanavalin A; D002772:Cholera Toxin; D000109:Acetylcholine