Z-DNA immunoreactivity in fixed metaphase chromosomes of primates.
Antibodies against Z-DNA bind to fixed metaphase chromosomes of man and Cebus albifrons (Platyrrhini, Primate). By indirect immunofluorescence and indirect immunoperoxidase techniques, a heavy staining is detected in some segments of chromosomes of C. albifrons. These segments correspond to R-band-positive heterochromatin, which has a high G + C-base content. Euchromatin of human and Cebus chromosomes show a weak and heterogeneous staining that consistently reproduces an R- and T-banding pattern in both species. Because chromosome homologies previously were demonstrated between these distantly related species by chromosome banding, our results suggest that Z-DNA has been conserved during the course of primate evolution.
D000818:Animals; D002428:Cebus; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000502:physiology*; D004247:DNA / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology*; D008677:Metaphase; D009690:Nucleic Acid Conformation; D012879:Skin Physiological Phenomena; D013045:Species Specificity
D004247:DNA