Stathmokinetic measurement of tumour cell proliferation in relation to vascular proximity.
In this study the metaphase-arrest method with vincristine (VCR) was used, in a transplanted C3H mouse mammary tumour, to study cell proliferation relative to distance from a blood vessel. The metaphase-arrest method has a number of advantages over methods involving [3H]TdR labelling for such a study. The cell birth rate (kB) was shown to be inversely related to distance from a capillary in corded areas of tumour. Penetration of VCR, even into perinecrotic areas of tumour, appeared to be sufficient to achieve effective metaphase arrest.
D000230:Adenocarcinoma / Q000098:blood supply* / Q000503:physiopathology; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D004261:DNA Replication; D005260:Female; D006652:Histological Techniques; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply* / Q000503:physiopathology; D051379:Mice; D008809:Mice, Inbred C3H; D008940:Mitotic Index / Q000187:drug effects; D014750:Vincristine / Q000494:pharmacology*
D014750:Vincristine