Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D.
A heat-stable enterotoxin produced by a strain of enterotoxigenic Escherichia coli 18D was purified by ion-exchange and reversed-phase high-pressure liquid chromatography. The amino acid sequence of the purified toxin was determined by Edman-degradation and a combination of fast atom bombardment mass spectrometry and carboxypeptidase digestion to be Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr.
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000302:isolation & purification; D002845:Chromatography; D004768:Enterotoxins / Q000302:isolation & purification; D004926:Escherichia coli / Q000032:analysis*; D006358:Hot Temperature; D013058:Mass Spectrometry; D008970:Molecular Weight
D001427:Bacterial Toxins; D004768:Enterotoxins