Potassium efflux from frog oocytes: effects of temperature, preincubation and injection of a gelatin bead.
42K efflux was studied in frog oocytes. 17 of 43 cells had only a single slow exponential exchange of 42K, while the remainder had a curvature of 42K exchange with one or more fast fractions preceding the slow exponential one. This variability in the characteristic of 42K efflux is not associated with evident damage, occurs at both 5 degrees and 25 degrees C., is independent of the method of preincubation, and occurs whether or not the cells are injected with a gelatin bead. Most of the fast-exchange fraction of K is not caused by follicle cells or other extraoocytic sources. These results may help explain some of the apparently conflicting results reported in studies of K exchange in amphibian oocytes.
D000818:Animals; D001692:Biological Transport; D005260:Female; D005780:Gelatin / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D011894:Rana pipiens; D013696:Temperature; D013997:Time Factors
D005780:Gelatin; D011188:Potassium