Bacteriophage MS2 lysis protein does not require coat protein to mediate cell lysis.
We inserted all but the extreme 5' end of a DNA copy of the bacteriophage MS2 lysis gene downstream of a lac-induced promoter on a multicopy plasmid. Upon induction, cells harboring this plasmid began to lyse, showing that phage coat protein is not required for the lytic process itself.
D001433:Bacteriolysis; D002213:Capsid / Q000502:physiology*; D004926:Escherichia coli; D008565:Membrane Proteins / Q000502:physiology*; D010957:Plasmids; D012317:RNA Phages / Q000032:analysis*; D014169:Transformation, Bacterial; D014764:Viral Proteins / Q000502:physiology*
D008565:Membrane Proteins; D014764:Viral Proteins; C037227:lysis protein, Enterobacterio phage MS2