title: Crystallization and 7 A resolution electron density map of the large fragment of Escherichia coli DNA polymerase I.
abstract: Crystals of the large fragment of Escherichia coli DNA polymerase I have been grown that diffract to better than 2.8 A resolution. They are in tetragonal space group P4(3) with a = b = 104.1 A, c = 86 A. A 7 A resolution map shows the protein to consist of two domains and to be mostly alpha-helical. The active site has been located by binding nucleoside monophosphates.
mesh_terms: D001665:Binding Sites; D003460:Crystallization; D003461:Crystallography; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli / Q000201:enzymology*; D009711:Nucleotides
chemical_list: D009711:Nucleotides; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase