Effect of venom gland extracts of the South American brown spider, Loxosceles laeta, on in vitro protein synthesis.
There was no effect of the venom extract on incorporation of phenylalanine into protein from phenylalanyl-tRNA. In contrast, the esterification of amino acids with tRNA was inhibited by the venom extracts in a dose-dependent manner, with an apparent preservation of the covalent structure of aminoacyl-tRNA.
D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D005088:Exocrine Glands / Q000032:analysis; D066298:In Vitro Techniques; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D051381:Rats; D013111:Spider Venoms / Q000494:pharmacology*; D014020:Tissue Extracts / Q000494:pharmacology
D001180:Arthropod Venoms; D012346:RNA, Transfer, Amino Acyl; D013111:Spider Venoms; D014020:Tissue Extracts; D010649:Phenylalanine