Babesia bovis: isolation of a protective antigen by using monoclonal antibodies.
Three distinct monoclonal antibody-producing hybridomas have been produced against a partly purified protective fraction of Babesia bovis. All three stain the parasite or infected erythrocytes or both in precise and different manners when fluorescent-antibody techniques are used. The relevant antigens for each monoclonal antibody were isolated by immunoadsorption, their native molecular weights being 1.3 X 10(6), 180 X 10(3), and 44 X 10(3). Each antigen reacted in serological assays with homologous and heterologous bovine antisera to B. bovis. Susceptible splenectomized calves were immunized twice, 4 weeks apart, with the respective antigens and were challenged with virulent homologous organisms 2 weeks later. Strong protective immunity was induced by the antigen with a molecular weight of 44 X 10(3), but no significant protection was induced by either of the other two antigens.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000517:prevention & control; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007114:Immunization; D007163:Immunosorbent Techniques; D051379:Mice; D008970:Molecular Weight
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens