Alcohol dehydrogenase-coupled spectrophotometric assay of plasmalogenase.
Plasmalogenase has been assayed by conversion of the fatty aldehydes, released by hydrolysis of the vinyl ether bond of plasmalogens, to long-chain alcohols by horse liver alcohol dehydrogenase. The reaction was followed spectrophotometrically by measuring the oxidation of NADH. The assay is sufficiently sensitive to enable plasmalogenase activity to be determined in isolated oligodendroglia and derived membranes and in brain microsomal membranes using 50-250 micrograms protein.
D000429:Alcohol Oxidoreductases; D000818:Animals; D002417:Cattle; D006867:Hydrolases / Q000032:analysis*; D051379:Mice; D009243:NAD / Q000032:analysis; D009836:Oligodendroglia / Q000201:enzymology; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D009243:NAD; D000429:Alcohol Oxidoreductases; D006867:Hydrolases; C022403:plasmalogenase