Circulating digitalis-like substance is increased in DOCA-salt hypertension.
Blood pressure and digitalis-like substance were measured in the plasma of control, salt-treated, and DOCA-salt treated rats. Blood pressure in DOCA-salt treated rats was significantly higher than that of either control or salt-treated animals. Digitalis-like activity was measured by two methods, radioimmunoassay for digoxin, and a receptor binding assay employing a rat brain synaptosomal membrane fraction. Digoxin-like immunoreactivity in plasma was not detected in either control or salt-treated rats, but was detected in DOCA-salt treated rats. Receptor binding activity in salt-treated rats was slightly but significantly higher than that of control rats. In DOCA-salt treated rats, receptor binding activity was significantly higher than that of salt-treated rats. Partial purification of the digitalis-like substance in plasma was performed by gel filtration using Sephadex G-25. Two peaks containing digoxin-like immunoreactivity were observed. Receptor binding activity, as well as Na+-K+ ATPase inhibitory activity, was detected only in the second peak, in which approximately 70% of the digoxin-like immunoreactivity was eluted. These results indicate that a circulating digitalis-like substance is increased in DOCA-salt hypertension.
D000818:Animals; D002850:Chromatography, Gel; D003429:Cross Reactions; D003900:Desoxycorticosterone; D004071:Digitalis Glycosides / Q000097:blood* / Q000494:pharmacology; D004077:Digoxin / Q000097:blood*; D006973:Hypertension / Q000097:blood* / Q000139:chemically induced; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000378:metabolism; D012965:Sodium Chloride; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D004071:Digitalis Glycosides; D011955:Receptors, Drug; C027082:digitalis receptor; D003900:Desoxycorticosterone; D012965:Sodium Chloride; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase