Comparison of manually performed Microtiter plate and semiautomated (cuvette) indirect enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine.
Standardized enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine were developed, using the traditional Microtiter plate method and a method based on semiautomated processor-analyzer instrumentation. The results of the 2 procedures were not significantly different when the titers of positive and negative anti-Mycoplasma hyopneumoniae standard reference sera were determined in triplicate in 3 separate experiments. Although both methods yielded reproducible results, the instrument-assisted enzyme-linked immunosorbent assay would be preferable if large numbers of samples are to be tested.
D000818:Animals; D001331:Automation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007124:Immunoenzyme Techniques; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*