title: Mitochondrial transfer RNA genes from fungi (Aspergillus nidulans) and plants (Lupinus luteus) are transcribed in Xenopus laevis oocyte nuclei.
abstract: Three plasmids containing Aspergillus nidulans mitochondrial transfer RNA genes were microinjected into the nucleus of Xenopus laevis oocytes. Plasmids that contained a single tRNACys gene or two tRNA genes (Arg and Asn) yielded tRNA-sized transcripts. Plasmids containing cloned Lupinus mitochondrial tRNA genes were also transcribed and processed in X. laevis nuclei.
mesh_terms: D000818:Animals; D001233:Aspergillus nidulans / Q000235:genetics*; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D005796:Genes; D008845:Microinjections; D008928:Mitochondria / Q000648:ultrastructure; D009865:Oocytes / Q000648:ultrastructure; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D012331:RNA, Fungal; D012343:RNA, Transfer