Morphogenesis of nuclear polyhedrosis virus from Autographa californica in a cell line from Mamestra brassicae (cabbage moth). Further aspects on baculovirus assembly.
Cultures of cell line IZD-MB 0503 from Mamestra brassicae were inoculated with nonoccluded nuclear polyhedrosis virus of Autographa californica (AcNOV). At 1 h postinoculation (p.i.) nucleocapsids were found in the cytoplasm near nuclear pores and within the nucleoplasm. Formation of virogenic stroma was observed at 7 h p.i. The first short empty capsids were seen at 10 h postinoculation (p.i.), followed by partially and completely filled nucleocapsids (11-12 h p.i.) with most capsids filled at 12 h and later. This suggests that nucleocapsid components, such as capsid and DNA-core, assemble in successive stages rather than simultaneously. Viral progeny, being released from the cells by budding, were observed at 13 h p.i.
D000818:Animals; D002213:Capsid / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D007304:Insect Viruses / Q000254:growth & development* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009024:Morphogenesis; D009036:Moths; D009685:Nuclear Envelope / Q000382:microbiology; D014617:Vacuoles / Q000382:microbiology; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins