Dk-restricted antiinfluenza cytotoxic T-cell clone loses one of its two alloreactivities.
K6, an influenza-specific cytotoxic T (Tc)-cell clone derived from C3H/He mice, was Dk-restricted. Further analysis showed that K6 also recognized Dd and Dq as allotargets. The clonal nature of the three recognition specificities was confirmed by cold-target cell inhibition. After extensive in vitro selection, K6 lost the ability to lyse Dd cells while retaining both other specificities. The role of the Lyt-2 molecule in antigen-specific self-H-2-restricted vs allospecific recognition was investigated by inhibition with the Lyt-2-specific monoclonal antibody 53-6.7. Both activities were proportionately inhibited, indicating that the Lyt-2 molecule is equally important for both recognition specificities of this Tc-cell clone.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D009980:Influenza A virus / Q000276:immunology*; D007519:Isoantigens / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D007519:Isoantigens