A 45 000 molecular weight human renin precursor is synthesized in a cell-free translation system.
Human kidney mRNA species were isolated and fractionated through a continuous sucrose gradient ultracentrifugation. mRNA fractions were translated by using a rabbit reticulocyte lysate and [35S]methionine as tracer. Double immunoprecipitation was carried out with highly specific anti-human renin and anti-rabbit gamma-globulin antisera. A 15S mRNA has been found to direct synthesis of a 45 000 molecular weight protein immunoprecipitable with anti-human renin. This protein is considered to be the ultimate precursor of renin (pre-prorenin).
D004792:Enzyme Precursors / Q000096:biosynthesis*; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012083:Renin / Q000096:biosynthesis*
D004792:Enzyme Precursors; D012333:RNA, Messenger; D012083:Renin