[Method of preparing durable supravital skin grafts].
A method for in vitro perfusion of human full-thickness skin grafts is described for the first time. A skin flap from the inguinal region, supported by A. circumflexa ilium superficialis and A. epigastrica superficialis, is incubated at 37 degrees C in physiological plasma exchange solution under an atmosphere of 95% O2 and 5% CO2. The main blood vessels of the skin graft are cannulated and perfused (s. table 1). The pressure of the perfusion medium is 4.5 kPa - 5.5 kPa and the flow rate 90 ml/h - 120 ml/h. The epidermis of the flap is incubated with H-3-thymidine. Our technique provides maintainance of cell vitality over at least 48 hours. The thymidine incorporation is not impaired. Our system enables us to study the action of different substrates on surviving human skin under constant conditions.
D006801:Humans; D008545:Melanoma / Q000601:surgery; D010477:Perfusion / Q000379:methods; D012867:Skin / Q000098:blood supply; D012878:Skin Neoplasms / Q000601:surgery; D016038:Skin Transplantation; D013524:Surgical Flaps
Methode zur Gewinnung langlebiger supravitaler Hautpräparate.