title: Does the lactose carrier of Escherichia coli function as a monomer?
abstract: The purified lactose carrier of Escherichia coli (product of the lac Y gene) is shown to be a monomer in detergent micelles of dodecyl-O-beta-D-maltoside. The negative-dominant phenotype of mutant carriers (lacY-d mutants) could not be verified by measurements of the rate of galactoside transport in lacY+/Y-d diploid strains. It is proposed that the membrane-embedded carrier functions as a monomer in galactoside-H+ symport.
mesh_terms: D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005697:Galactosides / Q000378:metabolism; D046911:Macromolecular Substances; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008823:Micelles; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D027981:Symporters
chemical_list: D029968:Escherichia coli Proteins; D005697:Galactosides; C050221:LacY protein, E coli; D046911:Macromolecular Substances; D026901:Membrane Transport Proteins; D008823:Micelles; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease