Solid-phase enzyme immunoassay of urokinase using monoclonal antibodies.
Using two monoclonal antibodies directed against urokinase, we have developed a micro enzyme-linked immunosorbant assay (ELISA) to detect and measure urokinase in biological fluids. The system presents the following characteristics: simple and rapid procedure, reproducibility, sensitivity (urokinase levels down to 1 ng/ml) and evaluation of the enzyme in biological fluids such as urine, pleural effusions, and ascitic fluids without preliminary purification.
D000911:Antibodies, Monoclonal; D010450:Endopeptidases / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008832:Microchemistry / Q000379:methods; D008970:Molecular Weight; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis*
D000911:Antibodies, Monoclonal; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator