Induction of fibroblast proliferation by interleukin-1 derived from human monocytic leukemia cells.
Human interleukin-1 (IL-1), free of contaminating lymphokines, was isolated from cultures of purified monoblasts from a patient with acute monocytic leukemia. Partially purified IL-1 (diafiltration, ultrafiltration, and isoelectric focusing) stimulated proliferation of subconfluent human fibroblasts in vitro. Further purification of IL-1 by high-resolution gel filtration- and anion exchange-high performance liquid chromatography revealed that fibroblast proliferation activity could not be separated from IL-1 activity (thymocyte proliferation), suggesting that both activities are the properties of a single molecule. Fibroblasts and thymocytes exhibited a similar sensitivity to the proliferative effects of IL-1. These findings suggest that macrophages participating in inflammatory reactions in vivo might release IL-1, which could function to expand fibroblast populations at sites of inflammatory reactions, by acting as a fibroblast growth factor.
D002455:Cell Division; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004247:DNA / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification / Q000502:physiology*; D007948:Leukemia, Monocytic, Acute / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D003470:Culture Media; D007375:Interleukin-1; D014316:Tritium; D005346:Fibroblast Growth Factors; D004247:DNA; D013936:Thymidine