[Characterization of cell suspensions adhering to nylon obtained from mononuclear cells in toto or depleted of monocytes].
The aim of this work was to evaluate the effect of monocyte depletion on the preparation of B cell enriched suspensions for DR typing. Nylon fiber adherent-cells obtained from total, or monocyte depleted, MNC were identified using different surface markers and cytochemical staining (SIg, DR, ANAE). The results demonstrate that no significant improvement in B enrichment is obtained by monocyte depletion.
D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion; D005260:Female; D006680:HLA Antigens; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009283:Naphthol AS D Esterase; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D006680:HLA Antigens; D009283:Naphthol AS D Esterase
Caratterizzazione di sospensioni di cellule aderenti al nylon ottenute da cellule mononucleate in toto o deplete di monociti.