Determination of herpes simplex virus type-specific antibodies by solid-phase RIA on Helix pomatia lectin-purified antigens.
Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) antibodies in humans and animals were determined by a micro-solid-phase radioimmunoassay with Helix pomatia lectin/Sepharose 4B-purified antigens from HSV-1- and HSV-2-infected cells. A low but constant heterotypic reactivity was found with HSV-1 antigen, whereas HSV-2 antigen was type specific, as evidenced by (1) its reactivity with sera from HSV-2- but not with those from HSV-1-immunized animals, (2) its reactivity with monoclonal antibody to glycoprotein C but not with that to other HSV-2 glycoproteins, and (3) the negative results obtained in serum samples from 57 children, of whom 49 possessed HSV-1 neutralizing antibody. Antibody to HSV-2 (anti-HSV-2) was detected in serum samples from 14 of 16 subjects treated for genital herpetic lesions. The prevalence of anti-HSV-2 in women did not differ in the 25-35- and 36-45-year-old age groups but did correlate with the number of sex partners, with only 10% of women reporting one sex partner and 33% of those reporting more than 10 sex partners having anti-HSV-2.
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000276:immunology; D006801:Humans; D007114:Immunization; D037102:Lectins; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D011817:Rabbits; D011863:Radioimmunoassay; D012725:Sexual Behavior; D018139:Simplexvirus / Q000145:classification / Q000276:immunology*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D001426:Bacterial Proteins; D006023:Glycoproteins; C038095:Helix lectin; D037102:Lectins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C038535:glycoprotein gC, herpes simplex virus type 1