Collagen gel culture system and analysis of estrogen effects on mammary carcinogenesis.
The results obtained to date from studies dealing with the role of hormones, including estrogen, on growth of mammary epithelial cells inside the collagen gel are described. The collagen gel matrix culture system appears to be a suitable system to obtain in vivo-like effects of hormones on mammary cell in vitro. The results thus far indicate that prolactin along with progesterone or cortisol can stimulate mammary cell proliferation. Thus far, estrogen has not been found to be mitogenic in our in vitro system.
D000818:Animals; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D003094:Collagen; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004967:Estrogens / Q000633:toxicity*; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced*; D051379:Mice; D011388:Prolactin / Q000494:pharmacology
D003470:Culture Media; D004967:Estrogens; D004815:Epidermal Growth Factor; D011388:Prolactin; D003094:Collagen; D002772:Cholera Toxin