Cloning the Escherichia coli K-12 argD gene specifying acetylornithine delta-transaminase.
The argD gene of Escherichia coli was shown to be present in plasmids pLC2-28 and pLC3-11 of the collection of Clarke and Carbon [Cell 9 (1976) 91-99]. The gene was cloned into pBR322 as a 6.3-kb BamHI fragment. Enzyme determination showed that the cloned DNA contains the structural gene for acetylornithine delta-transaminase. The argD DNA was used as a probe in hybridization experiments which indicated that the argM gene resides in a duplicated portion of E. coli DNA that is highly similar to the argD region.
D001120:Arginine / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D010957:Plasmids; D000637:Transaminases / Q000235:genetics*
D001120:Arginine; D000637:Transaminases; C018731:acetylornithine transaminase