title: Response to Mycobacterium bovis BCG vaccination in protein- and zinc-deficient guinea pigs.
abstract: Groups of specific pathogen-free Hartley strain guinea pigs were vaccinated with viable Mycobacterium bovis BCG and maintained on isocaloric purified diets containing either 30 or 10% protein (ovalbumin) combined with either 50 ppm (microgram/g) or no added zinc. Seven weeks later the guinea pigs were skin tested with purified protein derivative and killed. Both protein and zinc deficiencies had a significant negative impact on growth of the guinea pigs. Both groups consuming the 10% protein diet also demonstrated significant reductions in hematocrit, total serum proteins, and serum albumin, as well as diminished spleen weight. Plasma zinc concentrations were reduced in both low-zinc groups to less than half the value observed in control guinea pigs. Protein deficiency, alone or combined with zinc deficiency, resulted in increased tissue levels of viable M. bovis BCG in the inguinal lymph nodes and subcutaneous vaccination nodule. These same groups exhibited significant impairment in the ability to mount a delayed hypersensitivity reaction. Phytohemagglutinin-driven polyclonal T cell blastogenesis in vitro was significantly diminished in the peripheral lymphocytes of both protein- and protein-zinc-deficient animals at low mitogen doses, but only in the protein-zinc-deficient guinea pigs as the dose of phytohemagglutinin was increased. These results suggest that dietary protein and zinc deficiencies, alone or combined, interfere with immunological responses of the host to vaccination with M. bovis BCG.
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D008198:Lymph Nodes / Q000382:microbiology; D008213:Lymphocyte Activation; D009163:Mycobacterium bovis / Q000254:growth & development; D010835:Phytohemagglutinins / Q000494:pharmacology; D011488:Protein Deficiency / Q000276:immunology*; D012867:Skin / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination; D015032:Zinc / Q000172:deficiency*
chemical_list: D001500:BCG Vaccine; D010835:Phytohemagglutinins; D015032:Zinc