Effects of temperature and dose level on in vivo dynamics of unlabelled bovine insulin in eels (Anguilla anguilla L.).
The in vivo dynamics of bolus-injected, unlabelled bovine insulin in eels (Anguilla anguilla L.), analysed by compartmental and noncompartmental methods, were found to be significantly influenced by acclimation temperature and insulin dose level. At 12 degrees, plasma clearance of insulin was essentially monoexponential, whereas at 22 degrees, the disappearance curves could be resolved into two exponential components. Metabolic clearance rate (MCR) and apparent distribution volume (Vdist) of insulin were significantly higher at 22 degrees than 12 degrees, and irrespective of temperature, MCR was more rapid at a lower (130 mU/kg) than a higher (260 mU/kg) dose, while Vdist was more variable in this respect. These results indicate that the dynamics of insulin in eels are temperature-sensitive, and that over the dose range employed, routes of insulin elimination are saturable. Of particular significance were the inordinately high values for derived Vdist indicating that following bolus injection, substantial loss of the hormone occurred.
D000064:Acclimatization; D000818:Animals; D002417:Cattle; D004524:Eels / Q000097:blood*; D007328:Insulin / Q000008:administration & dosage / Q000097:blood*; D013696:Temperature
D007328:Insulin