Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo.
Escherichia coli uvr- cells, prelabeled in their DNA, were infected with phage T4 denV+ or T4 denV- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.
D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D003854:Deoxyribonucleotides / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D013604:T-Phages / Q000235:genetics
D004269:DNA, Bacterial; D003854:Deoxyribonucleotides