Rapid enrichment of mouse natural killer cells by use of wheat germ agglutinin.
The separation of mouse T and B lymphocytes by differential agglutination with wheat germ agglutinin (WGA) also enriches natural killer (NK) activity 2-7-fold. NK cells are recovered within the agglutinated cell population indicating that NK cells bind WGA. This technique can be applied to endogenous or interferon-induced NK cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D007694:Killer Cells, Natural / Q000276:immunology; D037102:Lectins / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D011070:Poly I-C / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000276:immunology; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins; D011070:Poly I-C