Supraoptic neurons in the rat hypothalamo-neurohypophysial explant: double-labeling with Lucifer Yellow injection and immunocytochemical identification of vasopressin- and neurophysin-containing neuroendocrine cells.
By combining intracellular electrophysiology with double-labeled intracellular dye-marking and immunocytochemical identification of the same magnocellular neuroendocrine cell, we studied supraoptic neurons in the rat hypothalamo-neurohypophysial explant in vitro. This report examines neurophysiological and light microscopical features of vasopressin- and neurophysin-immunoreactive, pituitary-projecting supraoptic neurons.
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007030:Hypothalamo-Hypophyseal System / Q000166:cytology*; D008297:Male; D009474:Neurons / Q000648:ultrastructure; D009481:Neurophysins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013495:Supraoptic Nucleus / Q000166:cytology*
D009481:Neurophysins; D001127:Arginine Vasopressin