Comparison of the sensitivity of the protein A plaque assay and the immunofluorescence assay for detection of immunoglobulin producing cells.
This paper reports a comparative study on the sensitivity of the protein A plaque assay and the cytoplasmic immunofluorescence assay for detection of immunoglobulin (Ig) producing cells in cell suspensions of murine lymphoid organs. The protein A plaque assay was found to detect as many or several times more Ig producing cells than the immunofluorescence assay, depending on the age and antigenic load of the mice, and upon the Ig class and organ studied.
D000375:Aging; D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D005455:Fluorescent Antibody Technique; D006462:Hemolytic Plaque Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013205:Staphylococcal Protein A / Q000494:pharmacology
D013205:Staphylococcal Protein A