Cell-free synthesis of rat epidermal growth factor-like polypeptide.
Poly(A+)RNA isolated from the adult male rat salivary gland has been found to direct the synthesis of immunoreactive EGF polypeptide in the wheat germ cell-free translation system. When the immunoreactive translation products were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, a single radioactive peak which co-migrated with the standard 125I-EGF (mouse) was observed.
D000818:Animals; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000378:metabolism*
D011061:Poly A; D004815:Epidermal Growth Factor