Capillary gas chromatographic analysis of alditol acetates of neutral and amino sugars in bacterial cell walls.
Several improvements in the preparation of alditol acetates of neutral and amino sugars and in the preparation of glass capillary columns for their separation are described. Modifications in sample preparation permitted the simultaneous processing of multiple samples and eliminated extraneous background peaks. Efficient and inert columns were tailor-made for the separation of alditol acetates of neutral and amino sugars by leaching glass capillaries with aqueous hydrochloric acid and dynamically coating with SP-2330.
D000085:Acetates / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis*; D002473:Cell Wall / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013297:Streptococcus pyogenes / Q000032:analysis; D013402:Sugar Alcohols / Q000032:analysis*
D000085:Acetates; D000606:Amino Sugars; D011135:Polysaccharides, Bacterial; D013402:Sugar Alcohols