Development of an immunofluorescence focus assay for Ebola virus.
A 48-h indirect immunofluorescence focus assay for the quantitation of Ebola virus was developed, utilizing HeLa-229 cell monolayers. The dose dependency and the sensitivity of this assay as compared with conventional assays are reported. This indirect immunofluorescence focus assay can be used as a rapid, quantitative test for the detection of Ebola virus, an agent from Africa known to cause hemorrhagic fever.
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D029043:Ebolavirus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D012209:Rhabdoviridae / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral