Intact human erythrocytes prevent hydrogen peroxide-mediated damage to isolated perfused rat lungs and cultured bovine pulmonary artery endothelial cells.
Addition of untreated or glutaraldehyde-fixed human erythrocytes decreased hydrogen peroxide (H2O2)-mediated acute edematous injury in isolated rat lungs, H2O2-induced damage to cultured bovine pulmonary artery endothelial cells, and H2O2-dependent oxidation of reduced cytochrome C in vitro. The results suggest that intact erythrocytes can scavenge H2O2, and as a result, protect the lung and possibly other tissues from damage.
D000818:Animals; D002478:Cells, Cultured; D003574:Cytochrome c Group / Q000378:metabolism; D004727:Endothelium / Q000187:drug effects; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D011651:Pulmonary Artery / Q000187:drug effects / Q000473:pathology*; D011654:Pulmonary Edema / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains
D003574:Cytochrome c Group; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase