title: Purification of alpha 1-proteinase inhibitor by triazine dye affinity chromatography, ion-exchange chromatography and gel filtration on Fractogel TSK.
abstract: The purification of plasma proteins by affinity chromatography on triazine dye matrices can be optimised with regard to the triazine dye used as a group-specific ligand. A comparison by electrophoretic and immunological means of the results of affinity chromatography with human plasma on Fractogel TSK-Blue and Fractogel TSK-Red demonstrated that the Procion Red HE-3B-containing gel was able to adsorb more plasma constituents than the Cibacron Blue F3-GA gel. The preparation of alpha 1-proteinase inhibitor obtained after chromatography on Fractogel TSK-Red showed a higher degree of purity and could easily be further purified by ion-exchange chromatography on Fractogel TSK DEAE-650 and gel filtration on Fractogel TSK HW 55, without significant loss of biological activity.
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D014227:Triazines; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification*
chemical_list: D014227:Triazines; D000515:alpha 1-Antitrypsin; C020795:procion red HE-3B; C038830:DEAE affigel blue