The effect of selenium-deficiency on rat fat-cell glucose oxidation.
When rats are fed a selenium-deficient diet, the glutathione peroxidase activity of epididymal fat-cells decreases to 5-9% of that of control rats fed the same diet supplemented with 0.5 p.p.m. of selenium as sodium selenite. [1-14C]Glucose oxidation in fat-cells from rats fed a selenium-deficient diet is unresponsive to the action of t-butyl hydroperoxide, which stimulates 14CO2 formation from [1-14C]glucose 4-fold in control rats. Insulin enhances [1-14C]glucose oxidation and incorporation into lipids in fat-cells from both groups of rats; however, the response elicited is reduced in fat-cells prepared from selenium-deficient animals. The 'C-1/C-6 ratio' (ratio of glucose C-1 to glucose C-6 oxidized) is enhanced by insulin to a similar degree in fat-cells from both groups of animals. The stimulatory action of Zn2+ and dithiothreitol on [1-14C]glucose oxidation observed in fat-cells from selenium-supplemented rats is greatly reduced in fat-cells from selenium-deficient rats. [1-14C]Glucose oxidation in fat-cells from both groups of animals is highly sensitive to the stimulatory action of adenosine. It is concluded that the enhanced formation and glutathione-linked destruction of H2O2 plays, at the most, only a minor role in the stimulation of the flux of glucose through the pentose phosphate pathway elicited by insulin, although elimination of glutathione peroxidase activity may influence the action of insulin on glucose oxidation. Production and subsequent destruction of H2O2 may play an important role in the stimulatory action of Zn2+ and dithiothreitol on fat-cell [1-14C]glucose oxidation.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D008297:Male; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D005989:Glycerides; D007328:Insulin; D010545:Peroxides; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D012643:Selenium; D005947:Glucose