[Electron-radioautographic analysis of 3H-thymidine distribution in the cell nuclei of the Chinese hamster].
Three types of the label localization in the nuclei of Chinese hamster fibroblasts, growing for 9 and 13 hours with 3H-thymidine, were detected using electron microscopic autoradiography: 1. The label is relatively evenly distributed throughout the karyoplasm. 2. Silver grains are concentrated as stripes through the nucleus; a high label density is also found in the nuclear periphery and around the nucleolus. 3. The label is mainly concentrated over the condensed chromatin adjacent to the nuclear membrane. The cells labeled in the first half of S-phase and selected with colchicine in postsynthetic phase of the 1st and the 2nd cycles are characterized by the second and third types of label distribution. In the cell nuclei fixed in the postsynthetic period of the second cycle, the label localization in stripes is discontinuous. The results indicate that during cell transition from S to G2 the newly-synthetized DNA changes its localization in the nucleus. It is suggested that the second type of label distribution depends on the interphase chromosome concentration in definite zones of the nuclear volume after S-phase termination, and the third type label localization is connected with the formation of prophase chromosomes.
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D013936:Thymidine
Elektronno-radioavtograficheskiĭ analiz raspredeleniia 3H-timidina v iadrakh kletok kitaĭskogo khomiachka.