Rapid detection of Salmonella spp. by using Felix-O1 bacteriophage and high-performance liquid chromatography.
A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is detected by high-performance liquid chromatographic techniques.
D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D012475:Salmonella / Q000302:isolation & purification* / Q000502:physiology; D012483:Salmonella Phages / Q000502:physiology; D012486:Salmonella typhimurium / Q000302:isolation & purification*