[Monoclonal antibodies against human differentiated thymocyte antigens].
Hybridomas producing monoclonal antibodies against cortical thymocytes (IKO-11) and early T cell precursors (IKO-10) were obtained. Monoclonal IKO-11 antibodies reacted with 75 +/- 1.6% of thymocytes and IKO-10 with 2.5 +/- 0.5%. Thymocyte fractionation over 19-33% gradient showed IKO-10 antibodies to react with the first fraction containing large cells and IKO-11 antibodies with the 4th-6th fraction containing small cells. The antibodies did not react with peripheral blood cells from healthy donors but detected antigens in patients with T cell ALL. If IKO-10 antibodies reacted with T cell ALL, there was no IKO-11 antibody reaction and vice versa. IKO-10 antibodies recognized this antigen on the cell surface of T cell Molt-4, SKCO-3 and non-T, non-B ALL Reh-6 lines. IKO antibodies did not react with these cells.
D000293:Adolescent; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000941:Antigens / Q000276:immunology*; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005333:Fetus / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007945:Leukemia, Lymphoid / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens
Monoklonal'nye atitela protiv differentsirovochnykh antigenov timotsitov cheloveka.