Somatic cell genetics and flow cytometry.
Human genes coding cell surface molecules can be introduced into mouse host cells using a variety of somatic cell genetic techniques. Because these human gene products can be detected using indirect immunofluorescence on viable cells, the genes themselves can be monitored and manipulated using flow cytometry and sorting. In this paper, we review ways that we have used cell sorting to develop a somatic cell genetic analysis of the human cell surface.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D002459:Cell Fusion; D002469:Cell Separation; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005796:Genes; D005821:Genetic Techniques; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007370:Interferon Type I / Q000494:pharmacology; D051379:Mice; D014162:Transfection; D001613:beta 2-Microglobulin / Q000235:genetics
D000954:Antigens, Surface; D006680:HLA Antigens; D007370:Interferon Type I; D001613:beta 2-Microglobulin