Purification of rat liver enzymes involved in the oxidation of glyoxylate.
The use of Sepharose aminohexyl oxamate for the purification of glycolate oxidase and lactate dehydrogenase is described. The kinetics of both enzymes are reported in relation to their possible roles in the production of oxalate. A model is proposed in which glycolate oxidase in the peroxisomes and lactate dehydrogenase in the cytosol cooperate in the production of oxalate.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D005260:Female; D006038:Glyoxylates / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats
D006038:Glyoxylates; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; C020821:glycollate oxidase