Development of a microassay technique for neutrophil adherence.
The development of a microassay for neutrophil adherence is described. The nylon fibre column consisted of 10 mg of fibre inserted into a micropipette tip. A vacuum harvester was constructed to aspirate the neutrophil-containing fluid in the columns into disposable test-tubes for quantitation of neutrophil concentrations. In this way, the neutrophil requirement was reduced from 10(7) cells to 10(5) cells. A complete assay can be performed efficiently and rapidly using less than 1 ml of blood.
D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D007111:Immunity, Cellular; D007158:Immunologic Techniques / Q000295:instrumentation*; D007957:Leukocyte Adherence Inhibition Test / Q000295:instrumentation* / Q000379:methods; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D011190:Potassium Cyanide / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D012969:Sodium Fluoride; D011190:Potassium Cyanide; D013755:Tetradecanoylphorbol Acetate