[Effect of chronic alcohol consumption on activity of enkephalinase A in rat striatum, hypothalamus and midbrain].
A modified procedure is developed for estimation of encephalinase A activity in rough membrane material from rat brain; the accuracy of the procedure was about +/- 5% and sensitivity--0.2 X 10(-15)M of 3H-Tyr-Gly-Gly per min if labelled Leu-encephalin was used as a substrate. Activity of encephalinase A in midbrain and hypothalamus was increased by 10% and in striatum--by 25% after chronic consumption of alcohol within 6 weeks. Rates of specific binding of 3H-Leu-encephalin with opiate receptors in the brain departments studied of the alcohol-treated rats were similar to those of control values. Encephalinase A appears to participate in development of dependence on alcohol or tolerance to it.
D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D003342:Corpus Striatum / Q000201:enzymology*; D004195:Disease Models, Animal; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D007031:Hypothalamus / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008636:Mesencephalon / Q000201:enzymology*; D015260:Neprilysin; D051381:Rats
D010450:Endopeptidases; D015260:Neprilysin
Vliianie khronicheskogo potrebleniia alkogolia na aktivnost' éntsefalinazy A v striatume, gipotalamuse i srednem mozge krys.