Alpha-factor enhancement of hybrid formation by protoplast fusion in the yeast Saccharomyces cerevisiae.
Two a strains of Saccharomyces cerevisiae, carrying complementary genetic markers, were arrested for 3 h with alpha-factor. These were then protoplasted, prior to fusion using polyethylene glycol. The number of viable fusion products was enhanced by a factor of 20 as compared with unarrested controls.
D006824:Hybridization, Genetic / Q000187:drug effects*; D000072235:Mating Factor; D010455:Peptides / Q000494:pharmacology*; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*
D010455:Peptides; D000072235:Mating Factor