title: [Acid phosphatase in blood (substrate: alpha-naphthylphosphate): reference values and diagnostic significance].
abstract: We report the results obtained with a modification of Hillmann's method (1) for determination of the catalytic concentration of acid phosphatase in serum using alpha-naphthylphosphate as the substrate. In a group of 158 males aged 16-85 years, the upper limit of the reference range (95th percentile) for the total acid phosphatase was established as 4.7 U/l (37 degrees C) and 4.2 U/l (30 degrees C), respectively; the corresponding values for the tartrate-inhibited acid phosphatase were 1.6 U/l (37 degrees C) and 1.5 U/l (30 degrees C). The upper limit of the reference range (95th percentile) for the total acid phosphatase determined in 60 females aged 18-80 years was 3.7 U/l (37 degrees C) and 3.0 U/l (30 degrees C). The catalytic concentrations in men and women did not show any age-related differences. The catalytic concentration of the tartrate-inhibited acid phosphatase was determined with the substrates alpha-naphthylphosphate and p-nitrophenylphosphate in a group of 89 patients with prostatic carcinoma (stages C and D). In 74 of these patients, the concentration of the prostatic specific acid phosphatase was assayed by enzyme-immunoassay and radioimmunoassay. The sensitivity of the method with p-nitrophenylphosphate was found to be unsatisfactory (66%), while that obtained with the other methods was superior and intercomparable (approx. 90%). The results obtained with the two substrates (p-nitrophenylphosphate vs. alpha-naphthylphosphate) differed significantly.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D004796:Clinical Enzyme Tests; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009281:Naphthalenes; D009943:Organophosphorus Compounds; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012737:Sex Factors; D013379:Substrate Specificity
chemical_list: D009281:Naphthalenes; D009943:Organophosphorus Compounds; C014694:naphthyl phosphate; D000135:Acid Phosphatase
vernacular_title: Saure Phosphatase im Serum (Substrat: alpha-Naphthylphosphat): Referenzwerte und diagnostische Aussage.