title: Delta pH-dependent and -independent uptake of [3H]dopamine by synaptic vesicles isolated from rat brain.
abstract: The dopamine (DA)-translocating mechanism of synaptic vesicles isolated from rat brain has been studied in the presence of an artificially imposed delta pH on the vesicle membranes (acidic inside with respect to the external medium) without the aid of ATP-Mg2+. Under the experimental conditions, [3H]DA uptake by the synaptic vesicles was driven by two different, i.e. delta pH-dependent and -independent processes. Both processes appeared to be carrier-mediated based on the inhibition by NEM (N-ethylmaleimide), an -SH reagent, and by nomifensine, a DA uptake blocker at nerve terminals. The DA carrier of the vesicles was similar to that of the nerve terminal plasma membrane with respect to their susceptibility to nomifensine. The delta pH-dependent uptake was transient and most of the incorporated DA was easily lost from the vesicles. On the other hand, the delta pH-independent uptake increased with time and the amine was retained in the vesicles. The initial rate of the delta pH-independent uptake was lower than that of the delta pH-dependent one but their extents were comparable with each other. These results indicate that rat brain synaptic vesicles have a DA uptake system requiring no ATP hydrolysis. A preparation of synaptic vesicles used here exhibited an inwardly directed proton translocation in the presence of ATP-Mg2+ when monitored by following changes in the fluorescence of ANS (8-anilino-1-naphthalene sulfonate). However, the time course of the delta pH-generation was not influenced by the addition of 1 mM DA.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009627:Nomifensine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D009627:Nomifensine; D000255:Adenosine Triphosphate; D004298:Dopamine