Demonstration of electron-dense material in clear synaptic vesicles using cationic ferrocenyl compounds.
Electron-dense material in clear synaptic vesicles in rat cerebral cortex and neuromuscular junctions of frog cutaneous pectoris muscle was demonstrated by using ferrocenyl cationics. Electron-dense spots were usually attached to the inner surface of the vesicular membrane. Control experiments (treatment with Triton X-100 or cetylpyridinium chloride; enzyme digestion with trypsin, hyaluronidase, neuraminidase, sulfatase and beta-glucuronidase) suggested that the electron-dense material is a glycoprotein.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000648:ultrastructure*; D005260:Female; D005296:Ferrous Compounds; D008297:Male; D000075163:Metallocenes; D008854:Microscopy, Electron; D009132:Muscles / Q000294:innervation; D009469:Neuromuscular Junction / Q000648:ultrastructure*; D009942:Organometallic Compounds; D010873:Pinocytosis; D011896:Rana temporaria; D051381:Rats; D011919:Rats, Inbred Strains; D013572:Synaptic Vesicles / Q000648:ultrastructure*
D005296:Ferrous Compounds; D000075163:Metallocenes; D009942:Organometallic Compounds; C004998:ferrocene