Non-competitive enzyme immunoassay of human trypsin 1.
A sandwich enzyme immunoassay was developed for human pancreatic trypsin 1 using polystyrene balls coated with specific IgG as the first antibody and peroxidase-labeled IgG as the second antibody. The entire assay takes 6 h and the detection limit is 0.5 microgram/l. The assay can be performed on sera samples or on discs carrying dried blood spots. Good agreement was found with a radioimmunoassay kit. This simple assay could be widely applied to confirm the elevated immunoreactive serum trypsin described in newborn children with cystic fibrosis.
D000328:Adult; D000818:Animals; D001667:Binding, Competitive; D003550:Cystic Fibrosis / Q000175:diagnosis; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D007231:Infant, Newborn; D007531:Isoflurophate / Q000494:pharmacology; D011817:Rabbits; D014357:Trypsin / Q000097:blood* / Q000592:standards
D007531:Isoflurophate; D014357:Trypsin