[DNA of the polyhedras and granules of the turnip moth Agrotis segetum Schiff].
The properties of DNA of nuclear polyhedrosis (NPV) and granulosis (GV) viruses of turnip moth, Agrotis segetum Schiff were studied. These DNAs were found to differ in all their properties. NPV DNA belongs to the GC-type, GV DNA to AT type: the content of GC-pairs for NPV was 53.6% for GV 40.7%, the melting temperature in I SSC was 91.3 degrees C and 86.6 degrees C, respectively. The molecular weight of DNA first determined by 4 independent methods (electron microscopy, restriction analysis, zonal centrifugation, and renaturation kinetics) was found to be 68.7 X 10(6) for NPV and 85.9 X X 10(6) daltons for GV. On the basis of the results of studies on NPV and GV DNAs of turnip moth, the data obtained in this laboratory on the properties of their proteins and morphogenesis of polyhedras and granules, as well as the data from the literature, the status of genera is proposed for NPV and GV of insects belonging to the family Baculoviridae.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D007304:Insect Viruses / Q000032:analysis*; D007915:Lepidoptera / Q000382:microbiology*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009036:Moths / Q000382:microbiology*; D014771:Virion / Q000032:analysis
D004279:DNA, Viral
Izuchenie DNK poliédrov i granul ozimoĭ sovki Agrotis segetum Schiff.