Sterilization of MacConkey agar and CLED medium by gamma-radiation.
MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by gamma-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4 degrees C and compared well with freshly autoclaved media.
D000362:Agar; D001431:Bacteriological Techniques / Q000295:instrumentation; D001437:Bacteriuria / Q000175:diagnosis; D003037:Cobalt Radioisotopes; D003470:Culture Media; D005720:Gamma Rays; D013242:Sterilization
D003037:Cobalt Radioisotopes; D003470:Culture Media; D000362:Agar