The contribution of polycyclic aromatic hydrocarbons to the carcinogenic impact of emission condensate from coal-fired residential furnaces evaluated by topical application to the skin of mice.
The objective of this investigation was to identify the substances chiefly responsible for the carcinogenicity of the emission condensate from coal-fired residential furnaces. To realize this, the carcinogenic effect of various fractions was compared with that of an unseparated sample of emission condensate, tested in different doses. The probit and Weibull analysis of the results showed: (1) The condensate emitted from a coal fired residential furnace as well as the reconstituted condensate combining all fractions, provoked local tumors after repeated application to the dorsal skin of mice. The tumor incidence exhibited a clear cut dose-response relationship. (2) The fraction of polycyclic aromatic hydrocarbons (PAH) and thiaarenes with more than three rings accounted for almost the total carcinogenicity (109-118% compared with the total condensate) of the emission condensate from the coal-fired residential furnace. (3) The fraction containing azaarenes and nitroarenes (NO2-PAH) accounted only for 4-7% of the total carcinoma incidence of the emission condensate. (4) The content of benzo[a]pyrene (0.702 mg/g condensate) contributes 10-11% to the total carcinogenicity of the emission condensate. (5) The PAH-free fraction and the fraction containing PAH with 2 and 3 rings (together about 77% by wt) were almost ineffective. No cocarcinogenic activity of this fraction was obtained, since the total condensate, as well as the PAH-fraction consisting of more than three rings applied proportionally provoked about the same carcinoma incidence.
D000287:Administration, Topical; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000209:etiology*; D005591:Chemical Fractionation; D003031:Coal; D051379:Mice; D009369:Neoplasms / Q000139:chemically induced / Q000209:etiology*; D010212:Papilloma / Q000139:chemically induced / Q000209:etiology*; D011083:Polycyclic Compounds / Q000633:toxicity*; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D013223:Statistics as Topic; D013997:Time Factors; D014504:Urban Health
D000393:Air Pollutants; D001580:Benzopyrenes; D003031:Coal; D011083:Polycyclic Compounds; D012906:Smoke; D001564:Benzo(a)pyrene