Immunoglobulin classes in the garden lizard, Calotes versicolor.
Two classes of immunoglobulins have been purified from lizard serum using a combination of ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-200 or on Sepharose 6B. Lizard IgM is 2-ME sensitive and has an intact molecular weight similar to human IgM. On SDS-PAGE, reduced IgM dissociates into heavy and light chains of molecular weight 70,000 and 23,500 daltons respectively. Lizards also possess a 2-ME resistant, low molecular weight immunoglobulin designated as IgY similar to avian and amphibian IgY. IgY dissociates on SDS-PAGE to yield 59,500 dalton heavy and 26,000 dalton light chains. Antisera raised in rabbits to each of the two Ig classes could be made class-specific by cross-absorption, thus indicating that IgM and IgY represent distinct isotypes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D003429:Cross Reactions; D007075:Immunoglobulin M / Q000302:isolation & purification; D007136:Immunoglobulins / Q000145:classification* / Q000302:isolation & purification; D008116:Lizards / Q000276:immunology*; D008970:Molecular Weight
D000888:Antibodies, Anti-Idiotypic; C016512:IgY; D007075:Immunoglobulin M; D007136:Immunoglobulins