title: Isolation and preliminary characterization of circulating immune complexes from rabbits with experimental syphilis.
abstract: Immune complexes isolated from sera of rabbits with experimental, disseminated syphilis were found to have sedimentation coefficients greater than 19s. By radioimmunoblot assays, materials precipitated with 2.5% polyethylene glycol or chromatographed on DEAE-Affi-Gel Blue were found to contain albumin, C3, immunoglobulin M (IgM), IgG, and treponemal antigen(s), whereas control materials contained only albumin and IgG. When polyethylene glycol precipitation of immune complexes from syphilitic rabbits was followed by immobilization on protein A and acid elution, radioimmunoblots detected only IgG and treponemal antigen(s).
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001798:Blood Proteins / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity / Q000379:methods; D004195:Disease Models, Animal; D007136:Immunoglobulins / Q000302:isolation & purification; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D011863:Radioimmunoassay; D013587:Syphilis / Q000276:immunology* / Q000382:microbiology; D014210:Treponema pallidum / Q000276:immunology
chemical_list: D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D001798:Blood Proteins; D007136:Immunoglobulins