Evaluation of different cell cultures for the detection of heat-labile enterotoxins of Escherichia coli strains isolated from pigs.
The sensitivity of various cell cultures to heat-labile enterotoxins (LT) and Verocytotoxin (VT) of fifteen E. coli strains isolated from cases of pig colibacillosis in Poland was estimated and compared with the effect of enterotoxins of four standard E. coli strains. Of ten tested cell cultures, only the following were susceptible: CHO, Vero, GMK, and HeLa. Eight strains showed CTE in He La and CHO cells and five of these reacted in Vero cells. The results appear to suggest that some of the tested E. coli strains isolated from pigs produced VT enterotoxin. Morphological changes caused by the above mentioned E. coli toxins in Vero and GMK cells took the form of cell rounding, followed by cell dissolution.
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary; D004768:Enterotoxins / Q000302:isolation & purification*; D004926:Escherichia coli; D005260:Female; D006367:HeLa Cells; D007668:Kidney; D010053:Ovary; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology
D003603:Cytotoxins; D004768:Enterotoxins