Cryopreservation of marrow and stromal progenitor cells: use of long-term liquid culture as a measure of the recovery of renewable hematopoietic and stromal cells.
Long-term liquid culture was used to assess the broad functional integrity of murine bone marrow after cryopreservation. Cryopreserved and thawed marrow contained not only renewable hematopoietic stem cells, but also stromal progenitor cells capable of transferring the hematopoietic microenvironment to liquid culture. Long-term liquid culture may be a superior method of assessing the functional potential of cryopreserved marrow when compared to stem cell assays alone.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003584:Cytological Techniques; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D011309:Preservation, Biological