DNA damage, prophage induction and mutation by furazolidone.
Ultraviolet absorption data and thermal chromatography through hydroxyapatite (HAP) column revealed that furazolidone treatment of Vibrio cholerae cells produced more than 80% of DNA reversibly bihelical due to the formation of interstrand cross-links and the reaction obeyed a first order relation. Sensitivities of the Escherichia coli strains to the lethal action of the drug were in the order: AB 2480(uvr- rec-) greater than AB 2463(rec-) greater than AB 1886(uvr-) greater than AB 1157(repair proficient) or AB 4401(wild type). Furazolidone was 'Rec test' positive, produced dose-dependent prophage induction in E. coli cells and also dose-dependent streptomycin-resistance forward mutation in V. cholerae cells. The quantitative aspect and also the mode of furazolidone action on DNA were discussed.
D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005664:Furazolidone / Q000633:toxicity*; D009153:Mutagens; D009154:Mutation; D013045:Species Specificity; D013307:Streptomycin / Q000494:pharmacology; D014734:Vibrio cholerae / Q000187:drug effects* / Q000235:genetics
D004269:DNA, Bacterial; D009153:Mutagens; D005664:Furazolidone; D013307:Streptomycin