title: Mitochondrial NAD, L-lactate dehydrogenase and NAD, D-lactate dehydrogenase in the yeast Saccharomyces cerevisiae.
abstract: Mitochondrial NAD-linked L- and D-lactate dehydrogenase activities have been found in the yeast Saccharomyces cerevisiae grown at high (3%) but were absent at low (0.6%) glucose concentrations. The inhibition of mitochondrial protein synthesis by chloramphenicol and of primary respiration by antimycin A determines the appearance of the two activities even at low (0.6%) glucose concentration. Two respiratory deficient strains belonging respectively to the mit- class (which maintains mitochondrial protein synthesis) and to the rho- class (which loses mitochondrial protein synthesis) display the activities even at low (0.6%) glucose concentration. L- and D-lactate have been detected in the growth medium when the cultures had been undertaken at high glucose concentrations, but were absent at low glucose concentrations.
mesh_terms: D000693:Anaerobiosis; D000968:Antimycin A / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D009154:Mutation; D009243:NAD / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*
chemical_list: D009243:NAD; D000968:Antimycin A; D002701:Chloramphenicol; D007770:L-Lactate Dehydrogenase