[Microfluorimeter for chromosome study].
A microfluorimeter is described for estimating amounts of chemical components in individual cells or in chromosomes, and for registering these components' distribution along the chromosomes. To solve the former problem, photoelectrical photometry of the object's fluorescence intensity is employed. To solve the latter problem, the photographic technique is used--making photos of metaphase plates in automatic or semiautomatic regime of exposure, with the following measuring of the intensity of the chromosome image on the negative performed on the same apparatus. The results of estimation of DNA content in individual chromosomes of Muntiacus muntjak are presented.
D000818:Animals; D002875:Chromosomes / Q000032:analysis*; D003584:Cytological Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis; D003670:Deer; D003720:Densitometry / Q000295:instrumentation; D004581:Electronics / Q000295:instrumentation; D005470:Fluorometry / Q000295:instrumentation; D055095:Optics and Photonics / Q000295:instrumentation; D010783:Photometry / Q000295:instrumentation*
D004247:DNA
Mikrofluorimetr dlia issledovaniia khromosom.