Developmental change of protein constituents in chicken gizzards.
Developmental change of protein constituents of chick gizzard smooth muscle was described by the fluorescent antibody technique and two-dimensional polyacrylamide gel electrophoresis. Myosin heavy chain, tropomyosin, and desmin were immunohistologically detected in 5-day-old gizzard primordia, but myoglobin was detected after 19 days of incubation. Two-dimensional polyacrylamide gel electrophoresis revealed that most structural proteins including beta- and gamma-actin are synthesized almost simultaneously in the primordium, and accumulate in three patterns by which the proteins examined are classified: (1) gradually increasing protein (gamma-actin, tropomyosin, desmin), (2) abruptly increasing protein at a certain stage (myosin, myoglobin), (3) decreasing or constantly kept protein (tubulin, beta-actin). Based on the quantitative analysis of protein constituents, the nature of regulatory system of protein synthesis in smooth muscle and the possible functional difference between beta- and gamma-actin are discussed.
D000199:Actins / Q000032:analysis; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003893:Desmin; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian / Q000032:analysis / Q000254:growth & development*; D007381:Intermediate Filament Proteins / Q000032:analysis; D009218:Myosins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014335:Tropomyosin / Q000032:analysis
D000199:Actins; D003893:Desmin; D007381:Intermediate Filament Proteins; D011506:Proteins; D014335:Tropomyosin; D009218:Myosins