Ribosomal acidic proteins of eucaryotic cells. Isolation of a protein from pea seedlings equivalent to E. coli L7/L12.
By the method of ethanol-salt extraction with ion-exchange chromatography on CM-cellulose an acidic protein of pea 80S ribosomes was isolated. This protein located in the large subunit, had a molecular weight of 14 000 and an IEP of 4.7. The protein is partially phosphorylated, alanine-rich and has methionine at the N-terminal position. Based on these characteristics and on the comparative study of tryptic hydrolyzates of the plant protein and E. coli L7/L12, the protein so obtained is found to be homologous to the L7/L12 of the procaryotic ribosomes.
D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010944:Plants / Q000032:analysis*; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000648:ultrastructure; D013045:Species Specificity
D000596:Amino Acids; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12