Dual-substrate plate diffusion assay for proteases.
A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a range from 0.01 to greater than 100 IU/ml.
D001407:Bacillus / Q000201:enzymology*; D002364:Caseins / Q000378:metabolism; D003014:Clostridium botulinum / Q000201:enzymology*; D010450:Endopeptidases / Q000032:analysis*; D005780:Gelatin / Q000378:metabolism; D008722:Methods
D002364:Caseins; D005780:Gelatin; D010450:Endopeptidases