Purification of urokinase by monoclonal antibody affinity chromatography.
Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity.
D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification*; D006801:Humans; D014568:Urokinase-Type Plasminogen Activator / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator