Mitochondrial transfer RNA genes from fungi (Aspergillus nidulans) and plants (Lupinus luteus) are transcribed in Xenopus laevis oocyte nuclei.
Three plasmids containing Aspergillus nidulans mitochondrial transfer RNA genes were microinjected into the nucleus of Xenopus laevis oocytes. Plasmids that contained a single tRNACys gene or two tRNA genes (Arg and Asn) yielded tRNA-sized transcripts. Plasmids containing cloned Lupinus mitochondrial tRNA genes were also transcribed and processed in X. laevis nuclei.
D000818:Animals; D001233:Aspergillus nidulans / Q000235:genetics*; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D005796:Genes; D008845:Microinjections; D008928:Mitochondria / Q000648:ultrastructure; D009865:Oocytes / Q000648:ultrastructure; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012331:RNA, Fungal; D012343:RNA, Transfer