A unique T-cell monoclonal antibody with potential uses in autologous bone marrow transplantation.
A monoclonal antibody reactive with an antigen expressed by a T-cell subset has been produced. The antibody designated RPH-1 reacts with a subset of normal T-cells and thymocytes. The antibody was strongly reactive with blast cells from 3 patients with T-acute lymphoblastic leukemia (T-ALL) and also reacted with cells of 2 patients with non-T-ALL. The antibody did not react with leukemic cells from patients with acute myeloid leukemia (AML) or B-chronic lymphocytic leukemia (B-CLL)). RPH-1 reacted with 2 T-cell lines, no B-cell lines and no myeloid cell lines. Absence of toxicity to granulocyte macrophage colony forming units (CFU-GM) by RPH-1 in the presence of complement was demonstrated, and in view of strong reactivity with certain leukemic cells, potential application of RPH-1 as an immunological means of selectively removing tumour cells is indicated.
D000293:Adolescent; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D003602:Cytotoxicity, Immunologic; D006413:Hematopoietic System / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007938:Leukemia / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013234:Stem Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014182:Transplantation, Autologous
D000911:Antibodies, Monoclonal