Effect of 4-cholesten-3-one on 3 beta-hydroxysteroid dehydrogenase activity in rat adrenals.
4-Cholesten-3-one inhibited the activity of 3 beta-hydroxy-delta 5-steroid dehydrogenase (EC 1.1.1.145) in the microsomal cytoplasmic supernatant of rat adrenal homogenate when using 3 beta-hydroxy-5-androsten-17-one (DHA) as the substrate. The inhibition was sex dependent: in male adrenals it reached more than 51%, whereas in female glands it was not significant. The side-chain cleavage of [26-14C]-cholestenone in the mitochondrial rat adrenal preparation measured as labelled isocaproic acid was extremely low in comparison with [26-14C]-cholesterol.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002782:Cholestenes / Q000494:pharmacology*; D002783:Cholestenones / Q000494:pharmacology*; D005260:Female; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D051381:Rats / Q000502:physiology*; D011919:Rats, Inbred Strains
D002782:Cholestenes; D002783:Cholestenones; C000475:cholest-4-en-3-one; D015096:3-Hydroxysteroid Dehydrogenases