title: Neuronal cell-surface specific antigen(s) is expressed during the terminal mitosis of cells destined to become neuroblasts.
abstract: By immunizing mice with cells from embryonic chick motoneuron cultures, an antiserum was produced which recognizes an antigen(s) restricted to cell surfaces of most, or all, neurons. With the use of this antiserum, the appearance of neuron-specific antigenicity in cells of the embryonic spinal cord was examined by indirect immunofluorescence microscopy. The antigen or set of antigens reacting with this antiserum was first detectable in the neural tube of chick embryos at stage 15-16 (V. Hamburger and H.L. Hamilton, 1951, J. Morphol. 88, 49-92). In addition to the neuroblasts located in the mantle layer, some mitotic cells as well as some spindle-shaped cells in the germinal layer were antigen positive. Immunofluorescence microscopy combined with autoradiography revealed that none of the antigen-positive cells could be labeled with [3H]thymidine; thus they do not synthesize DNA, and none of the cells in the DNA synthetic phase expressed the antigen(s). As the neuroblasts do not synthesize DNA after they have differentiated from the germinal cells, we believe that the antigen-positive cells are differentiated elements and that the differentiation of membranes specific for neurons begins already before or during the terminal mitosis of cells which will be defined as neuroblasts.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D002454:Cell Differentiation; D002642:Chick Embryo; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008938:Mitosis; D009046:Motor Neurons / Q000276:immunology; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D013116:Spinal Cord / Q000196:embryology
chemical_list: D000954:Antigens, Surface; D007106:Immune Sera