Primary isolation of Trypanosoma cruzi by hemoculture: influence of glucose concentration.
Isolation of Trypanosoma cruzi in hemoculture was done in media with different glucose concentrations. The three strains studied developed into epimastigotes 2 weeks after seeding in media with less than 4.5 mM hexose. Inhibition of growth of trypanosomes in media containing a greater amount of glucose was not caused by the higher osmotic pressures. Cultures containing more than 4.5 mM glucose showed a greater depletion of pH and higher lactic acid production than did those with lower concentrations, resulting in damage to amastigote clusters or to initial epimastigote development. The maximum recommended level of glucose in the culture medium is determined by the detrimental acidification resulting from excessive quantities.
D000818:Animals; D001769:Blood / Q000469:parasitology*; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009997:Osmotic Pressure; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D007773:Lactates; D019344:Lactic Acid; D005947:Glucose