[Intracellular proteases of Thermoactinomyces vulgaris. 1. Occurrence of the proteases in the cell and dynamics of their formation during cultivation].
The investigation concerning the localization of proteolytically active enzymes in the cells of Thermoactinomyces vulgaris show that the enzymes are present in a dissolved form in the cellular extract (75%) as well as linked to the solid cell components (25%). The ratio of the activities of the soluble periplasmatical fraction to the soluble cytoplasmatical fraction to the insoluble cytoplasmatical membrane fraction was found to be 2:1:1. The formation of the proteins during the cultivation is measured by detecting the activity in the cellular extract. As soon as enough of biomass is present (weighable) in the medium, the protease activity can be detected in the cellular extract. It increases during the fermentation and amounts to 1 to 2% of the activity of the proteases in the medium between the 10th and the 24th hour. A correlation between the formation of biomass and enzymes does not exist.
D001426:Bacterial Proteins / Q000032:analysis; D003470:Culture Media; D005285:Fermentation; D008848:Micromonosporaceae / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D010447:Peptide Hydrolases
Intrazelluläre Proteasen aus Thermoactinomyces vulgaris. 1. Mitt. Vorkommen der Proteasen in der Zelle und Dynamik ihrer Bildung im Verlauf der Kulturführung.