Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture.
A new human endometrial cell line, RL95-2, derived from a Grade 2 moderately differentiated adenosquamous carcinoma of the endometrium has been passaged successfully in cell culture for more than 2 yr. The cells are characteristically epithelioid with well-defined junctional complexes, tonofilaments, filopodialike extensions, and surface microvilli. Nuclei are large, irregular, and invaginated frequently with multiple, prominent, lamellar nucleoli. The cells have a log phase doubling time of 22 to 34 h followed by continued growth at a reduced rate with no apparent plateau phase. They exhibit a strong tendency for piling up as well as for the formation of glandlike dome structures. Karyotypically the line is trisomic 8 (47,XX,+8) and has an 8% frequency of polyploidization. Both cytoplasmic and nuclear estrogen receptors are present. Antihuman alpha-keratin characterizes the cell line as epithelial, nonstromal. The RL95-2 cell line may provide a useful in vitro system for the investigation of the endocrine regulation of endometrial neoplasia.
D000230:Adenocarcinoma / Q000473:pathology* / Q000648:ultrastructure; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000648:ultrastructure; D002460:Cell Line; D046508:Culture Techniques; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007621:Karyotyping; D008855:Microscopy, Electron, Scanning; D011960:Receptors, Estrogen / Q000032:analysis; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure
D011960:Receptors, Estrogen