Procurement and biological significance of pure 11C-glucose.
Biological significance of 11C-glucose purity has been a controversial matter. In order to understand the contribution of impurities contained in the photosynthetic 11C-glucose, its analysis and biodistribution in mice was studied. Studies indicated the interference of impurities, particularly 11C-fructose in the injectate, with the quantitative evaluation of glucose metabolic rate in brain. Furthermore, results obtained offered a good basis for search of simpler and easier separation methodology. Namely, good selection and combination of commercially available high performance liquid chromatography (HPLC) column provided 11C-glucose with high radiochemical purity. The radiochemical yield achieved was 6-10% at 75 min after the end of bombardment including the HPLC purification. The imaging study by positron computed tomography in a rabbit showed the suitability of 11C-glucose as a radiopharmaceutical for the diagnosis of the brain function.
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging; D002250:Carbon Radioisotopes / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D051379:Mice; D010788:Photosynthesis; D011817:Rabbits; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed
D002250:Carbon Radioisotopes; D005947:Glucose