[Nuclear DNAse from rat brain].
Nuclear DNase specific for single-stranded DNA was obtained from the rat brain. The enzyme was isolated from a soluble protein fraction of total cell nuclei using gel filtration and ion-exchange chromatography. Nuclear DNase is Mg2+-dependent exodeoxyribonuclease and hydrolyzes homological, heterological and synthetic substrate at the same rate. It is established that nucleoside-5'-monophosphates are the major product of native and synthetic polydeoxyribonucleotides hydrolysis. It is supposed that the enzyme participates in reparation of neuron DNA.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology*; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D004926:Escherichia coli; D006868:Hydrolysis; D008297:Male; D012331:RNA, Fungal / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013950:Thymus Gland
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D012331:RNA, Fungal; D004247:DNA; D003851:Deoxyribonucleases
Iadernaia DNK-aza kletok golovnogo mozga krys.