Specific suppression of hybridoma immunoglobulin secretion by hapten-conjugated mouse IgG: a model of B-cell tolerance.
In an effort to establish a model system for studying B-cell tolerance, the effects of hapten-conjugated isologous mouse IgG on the secretion of antibody by a mouse hybridoma cell line were studied. The hybridoma cell line 35-12 (HC) secretes IgM antibody to the hapten dinitrophenyl (DNP). After HC cells are injected intraperitoneally into BALB/c mice, the cells initially undergo a marked reduction in the proportion of PFC/10(6) followed by a return of PFC to pretransfer levels by 7-10 days. Hapten-conjugated mouse IgG (DNP-MGG), which induces tolerance to DNP in normal mouse B cells, also induces suppression of HC PFC when administered within the first 2 days after the transfer. Administration of tolerogen either 2 days before injection of HC or after the PFC response has returned to preinjection levels fails to give suppression. Suppression is dose dependent and hapten specific since immunogenic hapten-carrier conjugates (e.g., DNP-KLH, DNP-Ficoll) and fluorescein-MGG are not suppressive. T cells may not be required for suppression since hybridoma cells inoculated into nude mice are also suppressed by DNP-MGG. These results suggest that hybridoma cells undergo a change from nonsecreting to secreting cells during in vivo growth and that administration of tolerogen during the nonsecreting stage inhibits antibody secretion.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001557:Benzenesulfonates / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D006462:Hemolytic Plaque Technique; D006825:Hybridomas / Q000276:immunology*; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000502:physiology*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008954:Models, Biological; D010954:Plasmacytoma / Q000276:immunology; D013997:Time Factors
D001557:Benzenesulfonates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C007488:dinitrobenzenesulfonic acid