Elimination of cross-reactivity to some component of erythrocytes in an antiserum to parathyroid hormone by absorption with fixed erythrocytes.
Cross-reactivity to some component of rat erythrocytes with an antiserum to parathyroid hormone (anti-PTH) was detected in fixed demineralized sections of bone using the peroxidase-antiperoxidase method. The cross-reactivity was eliminated by the preabsorption of anti-PTH with fixed, washed rat erythrocytes. This technique provides an easy and rapid method with which to eliminate cross-reactivity to erythrocytes whenever such a situation is encountered in immunohistochemical procedures.
D000042:Absorption; D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D010281:Parathyroid Hormone / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D010281:Parathyroid Hormone