Linkage analysis of the murine interferon-alpha locus on chromosome 4.
Southern blot analysis with a murine interferon-alpha2 (MuIFN-alpha2) cDNA probe revealed restriction fragment polymorphism of EcoRI- and HindIII-digested C57BL/6 and BALB/cDNA. The inheritance pattern of this polymorphism was examined using DNA from each of the seven recombinant inbred strains derived from C57BL/6 and BALB/c; the strain distribution pattern suggests linkage of INF-alpha genes to two histocompatibility loci on chromosome 4. Southern blot analysis of DNA from six bilinear congenic strains carrying different fragments of the BALB/c chromosome 4 on a C57BL/6 background showed linkage of IFN-alpha genes to the histocompatibility locus H-15. It can therefore be concluded that the IFN-alpha gene cluster is situated on chromosome 4 near the H-15 locus, between loci Mup-1 and b.
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D008040:Genetic Linkage; D006649:Histocompatibility Antigens / Q000235:genetics*; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011110:Polymorphism, Genetic; D013045:Species Specificity
D006649:Histocompatibility Antigens; D007370:Interferon Type I; D004262:DNA Restriction Enzymes