[Use of the immunoperoxidase method on paraffin sections].
Using an indirect immunoperoxidase technique, the methods of fixation and treatment of paraffin sections were explored for subsequent identification of myosin of smooth and skeletal muscle cells, actin, filamin, and immunoglobulins. Bouin's fluid and ethanol were found to be the most adequate fixators for immunomorphological analysis. Pronase treatment of paraffin sections increased the intensity of specific reactions considerably. Incubation of paraffin sections with normal goat serum and hydrogen peroxide decreased nonspecific staining of the sections.
D000818:Animals; D002417:Cattle; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology*; D008722:Methods; D009130:Muscle, Smooth / Q000276:immunology*; D009218:Myosins / Q000276:immunology*
D007074:Immunoglobulin G; D009218:Myosins
Primenenie immunoperoksidaznogo metoda na parafinovykh srezakh.