title: A pre-amplified reverse hemolytic plaque assay.
abstract: A pre-amplified reverse hemolytic plaque assay has been developed. Sheep red blood cells (SRBC) were coated with staphylococcal protein A (SPA) by the chromic chloride method. The protein A-coated SRBC (SPA-SRBC) was then pre-amplified with an appropriate amount of human class-specific Ig. The pre-amplified Ig-SPA-SRBC was used to detect class-specific Ig-producing cells. It was found that this pre-amplified reverse hemolytic plaque assay gave clearer, larger and more numerous hemolytic plaques which were easy to count and thus gave more accurate results.
mesh_terms: D000328:Adult; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology; D013205:Staphylococcal Protein A
chemical_list: D007106:Immune Sera; D007136:Immunoglobulins; D013205:Staphylococcal Protein A