Conjugated oestrogen during the menstrual cycle measured by a direct radioimmunoassay with an antiserum prepared against oestradiol-17-glucosiduronate-[C-6]-BSA conjugate.
Early morning and 24 h urine samples and serum were collected daily throughout the menstrual cycle in women. Urinary oestradiol-17-glucosiduronate (E2-17-G) was measured with a direct radioimmunoassay whose antiserum had been prepared against E2-17-G-[C-6]-bovine serum albumin conjugate and was very specific. On the average, E2-17-G in early morning and 24 h urine samples showed a prominent peak one day before the peak of urinary LH. The time relationship between these urinary E2-17-G and serum E2 levels and peaks was also investigated.
D000328:Adult; D002940:Circadian Rhythm; D003429:Cross Reactions; D004958:Estradiol / Q000031:analogs & derivatives* / Q000276:immunology; D004966:Estrogens, Conjugated (USP) / Q000276:immunology / Q000652:urine*; D005260:Female; D006801:Humans; D007106:Immune Sera; D008598:Menstruation; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine / Q000276:immunology*
D004966:Estrogens, Conjugated (USP); D007106:Immune Sera; C041796:estradiol-17-glucosiduronate-(C-6)-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D004958:Estradiol