A method for concentration of nucleoside triphosphates by coprecipitation with calcium fluoride.
A method for concentration of nucleoside triphosphates (NTP) is described. NTPs are quantitatively coprecipitated from the solution with calcium fluoride. The precipitate is separated by filtration through a membrane filter and NTPs are dissolved from the filter by immersing it in 0.5 N H2SO4. With this method also nucleoside diphosphates can be efficiently concentrated, but the method does not work with nucleoside monophosphates or cyclic AMP.
D000249:Adenosine Monophosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D002124:Calcium Fluoride; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004926:Escherichia coli / Q000032:analysis; D005459:Fluorides; D009711:Nucleotides / Q000032:analysis*; D011188:Potassium; D017680:Potassium Compounds
D009711:Nucleotides; D017680:Potassium Compounds; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; C033320:potassium fluoride; D002124:Calcium Fluoride; D005459:Fluorides; D011188:Potassium