[Cytosol malate dehydrogenase in Calicophoron ijimai trematodes and the effect of antiparasitic preparations on its activity].
The activity and properties of malate dehydrogenase (MDH; EC 1.1.1.37) and of "malic" enzyme (EC 1.1.1.40) in cytosole of the trematode C. ijimai were determined. The activity of MDH directed to oxaloacetate formation was shown to be 14 times and maximum velocity 13 times lower than that of the reverse reaction. The apparent KM was one order higher in the direct reaction. This confirms the possibility of glycolytic pathway in C. ijimai via CO2 fixation into phosphoenolpyruvate to form oxaloacatate which is readily eliminated by active MDH. The presence of "malic" enzyme in C. ijimai testifies to the occurrence of different pathways of succinate formation in this species.
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology*; D004353:Drug Evaluation, Preclinical; D006863:Hydrogen-Ion Concentration; D008291:Malate Dehydrogenase / Q000378:metabolism*; D010250:Paramphistomatidae / Q000187:drug effects / Q000201:enzymology*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
D000871:Anthelmintics; D008291:Malate Dehydrogenase
Tsitozol'naia malatdegidrogenaza v trematodakh Calicophoron ijimai i vliianie ne ee aktivnost' nekotorykh protivoparazitarnykh preparatov.