Effect of steroids on ACTH release from cultured pituitary cells of the rat.
Using a primary cell culture of rat anterior pituitary and corticotropin (ACTH) RIA, the effects of various steroids have been investigated. Dexamethasone inhibited the ACTH release stimulated by alpha-melanotropin and Pitressin (posterior pituitary extract), but did not influence the non-stimulated release. The effect of all the investigated steroids was significant; aldosterone exhibited a more marked inhibition than spironolactone. Simultaneous administration of aldosterone and spironolactone resulted predominantly in the manifestation of spironolactone action. A new effect of spironolactone has been demonstrated in vitro; the evaluation of its pharmacological importance requires studies in vivo.
D000324:Adrenocorticotropic Hormone / Q000032:analysis / Q000378:metabolism*; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011916:Rats, Inbred F344; D013148:Spironolactone / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology
D014667:Vasopressins; D013148:Spironolactone; D000450:Aldosterone; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones