traJ independence in expression of traT on F.
Studies of F plasmids in the same cell as a transfer-repressed IncFII R plasmid showed a 100 to 1,000-fold decrease in transfer and a 75-fold decrease in surface exclusion, but no detectable change was shown in the amount of TraTp synthesized. Moreover, a mutation in traJ on F which caused a 10(4)-fold reduction in transfer caused only a 3.6-fold decrease in TraTp. These two findings suggest that a significant amount of traT expression on F is independent of traJ. Furthermore, we showed, using immunoprecipitation of TraTp, that normal amounts of this protein could be present in the cell without producing normal levels of surface exclusion.
D001426:Bacterial Proteins / Q000096:biosynthesis; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D005144:F Factor; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D011815:R Factors
D001426:Bacterial Proteins