Effect of reduced membrane lipid fluidity on the biosynthesis of lipopolysaccharide of Escherichia coli.
A low molecular weight precursor of lipopolysaccharide was accumulated under conditions in which the membrane lipids of a fatty acid auxotroph of Escherichia coli were reduced to a non-fluid state. The lipopolysaccharide precursor was detected, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and autoradiography, in membranes isolated from cells which were pulse-labeled with N-acetyl-[1-14C]glucosamine. The precursor could be chased into mature lipopolysaccharide by returning the membrane lipids to a normal fluid state. Conversion of the precursor to lipopolysaccharide was inhibited by the presence of potassium cyanide or sodium arsenate. The processing of several outer membrane protein precursors, including the promatrix proteins, was also inhibited under these conditions. Preliminary characterization of the lipopolysaccharide precursor was undertaken.
D001426:Bacterial Proteins / Q000302:isolation & purification; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008560:Membrane Fluidity; D008563:Membrane Lipids; D008565:Membrane Proteins / Q000302:isolation & purification; D008970:Molecular Weight
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008563:Membrane Lipids; D008565:Membrane Proteins