title: Plasmid-controlled resistance to copper in Escherichia coli.
abstract: The copper resistance of a strain of Escherichia coli isolated from the effluent of a piggery where pigs were fed a diet supplemented with copper sulfate was controlled by a conjugative 78-megadalton plasmid designated pRJ1004. Plasmid pRJ1004 exhibited surface exclusion and incompatibility with standard plasmids belonging to incompatibility groups I1 and K. Sensitive strains of E. coli K-12 were unable to form colonies on nutrient agar containing more than 4 mM copper, whereas transconjugants which harbored pRJ1004 were able to form colonies on medium containing up to 20 mM copper.
mesh_terms: D003300:Copper / Q000494:pharmacology*; D019327:Copper Sulfate; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005819:Genetic Markers; D008970:Molecular Weight; D011815:R Factors; D014169:Transformation, Bacterial
chemical_list: D005819:Genetic Markers; D003300:Copper; D019327:Copper Sulfate