Monoclonal antibodies to human glomerular cells: a marker for glomerular epithelial cells.
A monoclonal antibody, PHM 5, directed against human glomerular epithelial cells, was prepared after immunisation of a mouse with isolated human glomeruli and fusion of spleen cells with a mouse myeloma. Binding of antibody to isolated glomeruli was detected by radioimmune indirect binding assay, and specificity for glomerular epithelial cells was shown using a four-layer peroxidase-antiperoxidase technique applied to epoxy resin sections of the human kidney cortex. PHM 5 appears to detect a human-specific cell surface carbohydrate antigen not previously described, and can be used to identify epithelial cells in renal biopsy sections and in culture outgrowths of isolated glomeruli.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D001666:Binding Sites, Antibody; D004848:Epithelium / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface