Dissociation of nucleoprotein complexes by chaotropic salts.
The effect of various anions in destabilizing yeast nucleoprotein complexes followed the order F- less than Cl- less than Br- less than ClO-4 congruent to Cl3CCOO-. Treatment of yeast nucleoproteins with 0.5 M NaClO4 resulted in removal of 80% of RNA. Based on the results, a simple method for effective separation of RNA from ribosomal particles is proposed and the mechanism of RNA dissociation by anions is also discussed.
D055598:Chemical Phenomena; D002621:Chemistry; D005656:Fungal Proteins; D009698:Nucleoproteins / Q000302:isolation & purification*; D012331:RNA, Fungal; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012492:Salts
D005656:Fungal Proteins; D009698:Nucleoproteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012492:Salts