Role of lymphokines in regulation of macrophage differentiation.
The regulatory mechanism of guinea pig lymphokines was investigated in regard to differentiation of myeloid cells to macrophages. The Ml-cell line, established from a myeloid leukemia of an SL-strain mouse, was induced to differentiate in vitro into mature macrophages possessing Fc receptors and the ability to phagocytize latex particles by treatment with crude lymphokines. Both concanavalin A- and antigen-induced lymphokines showed the differentiation-inducing factor (D factor) activity. However, macrophage migration inhibitory factor/ macrophage activation factor (MIF/MAF) purified by an immunoadsorbent column with anti-MIF antibody had no such an activity. The D-factor activity was detected in the lymphokine preparation that was not retained on the immunoadsorbent column. In contrast, colony-stimulating factor (CSF) was adsorbed to the immunoadsorbent column, and could be recovered in the purified MIF/MAF preparation. These findings suggest that the molecular entity of D factor is distinct from MIF/ MAF and CSF. A culture supernatant of guinea pig peritoneal macrophages activated with MIF/ MAF (CSF) exhibited strong D-factor activity. However, the supernatant possessed rather reduced CSF activity as compared to that of the original MIF/MAF (CSF) preparation. Thus, MIF/MAF may play an important role in macrophage differentiation by regulating the production of D factor or CSF from macrophages.
D000818:Animals; D002454:Cell Differentiation; D003115:Colony-Stimulating Factors / Q000502:physiology; D006023:Glycoproteins / Q000502:physiology*; D006131:Growth Inhibitors; D006168:Guinea Pigs; D007163:Immunosorbent Techniques; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000502:physiology*; D008262:Macrophage Activation; D008263:Macrophage Migration-Inhibitory Factors / Q000502:physiology; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D051379:Mice
D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D016215:Macrophage-Activating Factors