The role of class I MHC products in polyclonal activation of CTL function.
The generation of CTL function by agents such as lectins, oxidative chemicals, or serum components has been regarded as independent of class I MHC products, because the CTL thus generated are able to lyse virtually any allogeneic target. However, we show here that CTL activation by lectin requires interaction of the pre-CTL with a class I MHC product on the lectin-presenting cell. The lectin-presenting cell can be an irradiated syngeneic or allogeneic spleen cell or, alternatively, lectin can be seen on a neighboring pre-CTL. In either case, there is an absolute requirement for simultaneous (although probably unlinked) perception of a class I MHC product. These results suggest that restricted clonal CTL activation by antigen, and polyclonal activation by so-called "nonspecific" mitogenic agents, may proceed through similar if not identical pathways.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000961:Antilymphocyte Serum / Q000502:physiology; D001667:Binding, Competitive; D002999:Clone Cells / Q000276:immunology; D003208:Concanavalin A / Q000276:immunology / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D037102:Lectins / Q000276:immunology / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013234:Stem Cells / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000961:Antilymphocyte Serum; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D037102:Lectins; D003208:Concanavalin A