title: Fine structure analysis and surface characteristics of mouse mammary gland adenocarcinomas.
abstract: Mammary adenocarcinomas from C3H/HeJ mice carrying the mammary tumor virus were studied by means of light, transmission, and scanning electron microscopy. The histological appearance of the tumors was variable; however, 60% were classified as type B adenocarcinomas, while 20% were type A and 20% were composed of equal regions characteristic of both types. Four populations of tumor cells were observed with the TEM. The most abundant of these, primitive glandular cells, were characterized by large, regular, euchromatic nuclei and cytoplasm containing numerous free ribosomes, little rough endoplasmic reticulum, few mitochondria, small Golgi complex and a variable number of type A virus particles. Specialized glandular cells contained highly pleomorphic nuclei, many lysosomes, lipid droplets, multivesicular bodies, profiles of rough endoplasmic reticulum and granules resembling secretory proteins. Myoepithelial cells and dark glandular cells with abundant organelles, large Golgi complexes, dense cytoplasmic matrix and very heterochromatic nuclei were observed infrequently. The SEM revealed tumor cells to be variable in size, shape and surface characteristics. Most cells were rough in texture, displaying irregular ridges, small blebs and a few short microvilli. The contours of some cells were smooth, and a few cells had short, irregular microvilli on limited regions of their surfaces. Cells lining ducts within the tumor had microvilli on their apical surface, but the number, size, shape and distribution of microvilli varied considerably. Cells lining ducts from non-tumor-bearing animals displayed less variation in size, shape and surface morphology.
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000648:ultrastructure*; D000818:Animals; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology / Q000648:ultrastructure*; D008324:Mammary Tumor Virus, Mouse / Q000472:pathogenicity; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods