Metabolic activation of 1,2-dibromoethane to a free radical intermediate by rat liver microsomes and isolated hepatocytes.
A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, under hypoxic conditions, it has been possible to trap a free radical intermediate and identify it by using 13C-DBE. Inhibition experiments have demonstrated that the site of activation is the microsomal drug metabolizing system.
D000332:Aerobiosis; D000818:Animals; D001711:Biotransformation; D015946:Ethylene Dibromide / Q000378:metabolism*; D005609:Free Radicals; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D005609:Free Radicals; D006842:Hydrocarbons, Brominated; D015946:Ethylene Dibromide