Purification of patient-related allergens by means of bioaffinity chromatography on a sepharose anti-IgE patient IgE immunosorbent.
A method is described for bioaffinity chromatographic purification of patient-specific IgE reactive allergens by using sepharose coupled rabbit anti-human IgE antibodies, patient serum and an allergen extract. The conditions for this bioaffinity chromatographic procedure have been optimised, and it was shown that the nonspecific binding of serum proteins and antigens can be kept at very low levels by a high ionic strength. The specificity of the method has been determined by showing that allergens to which the patient did not react were not bound to the affinity matrix. We suggest that the isolated allergens could be used for more specific immunotherapy in the future.
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001798:Blood Proteins / Q000276:immunology; D002846:Chromatography, Affinity; D006801:Humans; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000276:immunology*; D011864:Radioimmunosorbent Test; D012685:Sepharose
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D001798:Blood Proteins; D007073:Immunoglobulin E; D012685:Sepharose