The use of monoclonal anti-idiotypic antibodies for studying antigen-specific T and B cell receptors.
Eight syngeneic HP-id specific for anti-GAT antibodies are tested for their capacity to detect idiotypes on GAT-specific T cell lines. Their affinity constants are between 5 X 10(-7) and 10(-8) molar-1 which permit significant binding analysis. They detect idiotopes expressed in anti-GAT sera from all the strains of mice tested and by 14 BALB/c HP-GAT. In addition, the distribution of the corresponding idiotopes among all allogeneic HP-GAT allow the identification of two groups of idiotopes. Some idiotopes are expressed by a minority of HP-GAT; the other idiotopes are expressed by all of them. Nevertheless, in spite of the broad molecular and genetic distribution of these idiotopes on anti-GAT antibodies, they are not expressed by three GAT-specific T cell lines from different genetic origins.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology; D011108:Polymers; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010455:Peptides; D011108:Polymers; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell; C020749:GAT