[Cyclic nucleotides and calcium ions in the activation of B-lymphocyte movement in mice by an anti-immunoglobulin serum].
It was shown that B lymphocyte motility activated by anti-immunoglobulin serum may be abrogated in a Na+-deficient medium and in a 10(-5)M trifluoperazine-containing medium but not in a Ca2+-deficient medium. The tetracycline fluorescence test demonstrated Ca2+ efflux from isolated B lymphocyte mitochondria due to Na+ exposure. The radioimmunoassay demonstrated the cGMP level to rise after exposure to anti-immunoglobulin serum. The Na+-dependent Ca2+ efflux from the mitochondria might be the main mechanism in anti-immunoglobulin serum activation of B lymphocytes and in the cGMP level rising.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002118:Calcium / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects; D007106:Immune Sera / Q000302:isolation & purification / Q000494:pharmacology*; D007114:Immunization / Q000379:methods; D007136:Immunoglobulins / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009712:Nucleotides, Cyclic / Q000502:physiology*; D011817:Rabbits; D014268:Trifluoperazine / Q000494:pharmacology
D007106:Immune Sera; D007136:Immunoglobulins; D009712:Nucleotides, Cyclic; D014268:Trifluoperazine; D000109:Acetylcholine; D002118:Calcium
Tsiklicheskie nukleotidy i iony kal'tsiia v aktivatsii podvizhnosti B-limfotsitov myshi antiimmunoglobulinovoĭ syvorotkoĭ.