Use of high-performance liquid chromatography in the analysis of retinyl and 3,4-didehydroretinyl compounds in tissue extracts of bullfrog tadpoles and goldfish.
HPLC (high-performance liquid chromatography) was used to analyse retinyl and 3,4-didehydroretinyl compounds in tissue extracts from goldfish and bullfrog tadpoles. Using silica columns (packed with 10-micron mu Porasil or 5-micron Ultrasphere particles) eluted with n-hexane (containing a small amount of dioxane or diethyl ether), the authentic all-trans retinyl and 3,4-didehydroretinyl palmitates, retinal and 3,4-didehydroretinal, retinol and 3,4-didehydroretinol were completely separated. Liver and eye extracts of the goldfish and bullfrog tadpoles had mainly esterified all-trans retinol and all-trans 3,4-didehydroretinol. In the liver, these vitamin A were conjugated to a number of fatty acids whereas in the eye, principally one fatty acid was used. Moreover, the relative proportions of all-trans retinol and all-trans 3,4-didehydroretinol (obtained by analysing the saponified esters) were significantly different between some of these body compartments.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D004952:Esters; D005123:Eye / Q000032:analysis*; D006054:Goldfish; D007814:Larva; D008099:Liver / Q000032:analysis*; D011892:Rana catesbeiana; D012172:Retinaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D012176:Retinoids / Q000032:analysis*; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives / Q000032:analysis
D004224:Diterpenes; D004952:Esters; D012176:Retinoids; D000084562:Retinyl Esters; C024366:vitamin A2; D014801:Vitamin A; C014794:retinol palmitate; C017184:dehydroretinal; D012172:Retinaldehyde