Lack of specificity of the C3-opsonized zymosan reagent for the assay of membrane complement receptors.
C3-opsonized zymosan particles (C3-zymosan) have been utilized as reagents for detection of C3 receptor bearing cells. However, in this communication we present evidence that C3-zymosan particles are not useful for this purpose in some species with certain macrophage populations because of non-specific binding of unopsonized zymosan.
D000818:Animals; D001665:Binding Sites; D003176:Complement C3 / Q000276:immunology*; D003170:Complement Pathway, Alternative; D006224:Cricetinae; D005260:Female; D006168:Guinea Pigs; D016177:Macrophage-1 Antigen; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D009895:Opsonin Proteins / Q000276:immunology*; D051381:Rats; D011951:Receptors, Complement / Q000032:analysis*; D012397:Rosette Formation; D015054:Zymosan / Q000378:metabolism
D003176:Complement C3; D016177:Macrophage-1 Antigen; D009895:Opsonin Proteins; D011951:Receptors, Complement; D015054:Zymosan