A solid phase C1q enzyme assay for circulating immune complexes adapted for routine clinical laboratory testing.
An assay for circulating immune complexes (CIC) that is adaptable to a routine clinical laboratory is described. This solid phase C1q (SP C1q) enzyme immunoassay (EIA) uses commercially available reagents and is reproducible with CV% of less than 20% in the abnormal range. We also demonstrate the specificity of the assay for detecting clinically relevant dsDNA-anti-DNA immune complexes.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000032:analysis*; D003166:Complement Activating Enzymes / Q000592:standards; D015922:Complement C1q; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D007074:Immunoglobulin G / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology; D013696:Temperature; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D015922:Complement C1q; D003166:Complement Activating Enzymes