title: Amino acid composition and zinc content of milk-clotting protease from Bacillus mesentericus strain 76.
abstract: The milk-clotting protease from Bacillus mesentericus strain 76 is free of carbohydrate and lacks cysteine and cystine. The amino acid composition indicates a single peptide chain with 304 residues. Five amino acids--aspartic acid, threonine, serine, glycine and alanine--represent one-half of the total residues. The enzyme contains 35 aromatic amino acids and 103 ionic amino acids. The observed constant value of the ratio Menzyme:Azinc is 1:1 for active and acid denatured enzyme preparations, indicating that 0.1 M acetic acid causes denaturation of the enzyme but it can not eliminate the zinc ions.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D016282:Aspartic Acid Endopeptidases; D001407:Bacillus / Q000201:enzymology*; D002241:Carbohydrates / Q000032:analysis; D010450:Endopeptidases / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000032:analysis
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C022517:rennin-like enzyme (Aspergillus ochraceus); D015032:Zinc