Induction of ornithine decarboxylase in guinea-pig lymphocytes and its relation to phospholipid metabolism.
Treatment of lymphocytes with exogenous phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3.) derived from Clostridium perfringens at concentrations similar to those which induced ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activity produced diacylglycerol and phosphatidate. A divalent cation ionophore, A23187, and phytohemagglutinin induced not only diacylglycerol formation, but also ornithine decarboxylase activity. Dibutyryl cAMP inhibited both diacylglycerol formation and ornithine decarboxylase induction to a similar extent in phytohemagglutinin-stimulated lymphocytes, but stimulated them somewhat in ionophore A23187-activated lymphocytes. This suggests that the activation of intracellular phospholipase C and the formation of diacylglycerol is involved in ornithine decarboxylase induction in lymphocytes.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D003016:Clostridium perfringens / Q000201:enzymology; D004075:Diglycerides / Q000096:biosynthesis; D011756:Diphosphates / Q000378:metabolism; D004790:Enzyme Induction; D006168:Guinea Pigs; D007700:Kinetics; D008198:Lymph Nodes / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis*; D010738:Type C Phospholipases / Q000378:metabolism
D004075:Diglycerides; D011756:Diphosphates; D010743:Phospholipids; D000001:Calcimycin; D003994:Bucladesine; D010738:Type C Phospholipases; D009955:Ornithine Decarboxylase