title: [Action of sulfochlorantine on cell-free systems of Escherichia coli].
abstract: Sulfochlorantine containing active chlorine was shown to produce a general toxic action inactivating a number of Escherichia coli enzyme systems involved in protein biosynthesis. DNA synthesis catalysed by calf thymus DNA polymerase alpha was repressed by 50% and the synthesis of aminoacyl-tRNA catalysed by aminoacyl-tRNA synthetases was repressed by 70% in the cell-free systems at the lethal concentration of the bactericide (0.01% and higher). Sulfochlorantine produced the strongest inhibiting action on the ribosomal step of protein biosynthesis, inhibiting the poly(U)-directed polyphenylalanine formation by 95% at the sublethal concentration of the bactericide (0.005%).
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors; D002384:Catalysis; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004269:DNA, Bacterial / Q000037:antagonists & inhibitors; D004338:Drug Combinations / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D006827:Hydantoins / Q000494:pharmacology*; D011122:Polyphosphates / Q000494:pharmacology*; D012346:RNA, Transfer, Amino Acyl / Q000037:antagonists & inhibitors; D012270:Ribosomes / Q000187:drug effects / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D006827:Hydantoins; D011122:Polyphosphates; D012346:RNA, Transfer, Amino Acyl; C022876:sulfochloranthine; D004257:DNA Polymerase II
vernacular_title: O deĭstvii "Sul'fokhlorantina" na beskletochnye sistemy Escherichia coli.