Expression of cell surface determinant(s) on murine myeloma stem cells and hematopoietic stem cells.
Murine B cell lymphomas and myelomas were examined for the expression of a determinant previously found exclusively on normal pluripotent stem cells colony-forming unit-spleen (CFU-s). This determinant(s), which is defined by a rabbit antimouse brain antiserum (R alpha MB), is present on the tumor stem cell population of some but not all B cell neoplasms examined. The determinant is not detected on tumor cells of the macrophage or T cell lineage. Absorption of the activity in R alpha MB with myeloma cells, concomitantly removed reactivity with the normal stem cell, CFU-s, and the myeloma stem cell, plasmacytoma CFU-s. Sorting analysis further showed that the antigen was diminished within a positive tumor population as cells acquired the capacity to secrete immunoglobulin. These studies suggest that this normal stem cell-associated antigen may also be an early differentiation antigen for the B cell lineage, and is expressed on some stem cells of B cell tumors.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000032:analysis*; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface