The selection of malignantly transformed rat liver cells using a hormonally defined medium.
A hormonally defined medium consisting of a 1:1 mixture of Dulbecco's modified Eagle's medium and nutrient mixture F12 supplemented with 50 micrograms/ml each of insulin and transferrin was found to grow late passage malignantly transformed cells of the RL-PR-C rat liver cell culture. The same medium formulation would not grow the early passage, normal, diploid counterpart of the RL-PR-C cell culture. When mixtures of the early and late passage cells were made, only the late passage cells would grow, thus providing a selection system for the late passage cells.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D004848:Epithelium / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008113:Liver Neoplasms / Q000473:pathology; D051381:Rats; D014168:Transferrin / Q000378:metabolism
D003470:Culture Media; D007328:Insulin; D014168:Transferrin