title: Application of the immunoperoxidase technique to formalin fixed, paraffin embedded kidney biopsies.
abstract: Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence.
mesh_terms: D001706:Biopsy; D003176:Complement C3 / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007674:Kidney Diseases / Q000276:immunology*
chemical_list: D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005340:Fibrinogen