Stimulation of prostaglandin E and thromboxane synthesis in macrophages by purified C3b.
Complement cleavage product C3b was examined for its effect on macrophages. Albumin-elicited guinea pig peritoneal macrophages kept in short-term culture were challenged with purified homologous C3b, and release of oxygen and arachidonic acid metabolites was studied. C3b at concentrations ranging from 20 to 80 micrograms/ml induced synthesis of large quantities of the arachidonic acid cyclooxygenation products thromboxane B2 (TXB2) and prostaglandin E (PGE) but failed to trigger an oxidative burst. Our findings link together macrophages, complement, and arachidonate metabolites as major participants in inflammatory responses.
D000818:Animals; D003179:Complement C3b / Q000502:physiology*; D004731:Endotoxins / Q000037:antagonists & inhibitors; D006168:Guinea Pigs; D007163:Immunosorbent Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010101:Oxygen Consumption; D011112:Polymyxin B / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*
D004731:Endotoxins; D011458:Prostaglandins E; D013931:Thromboxanes; D013929:Thromboxane B2; D003179:Complement C3b; D011112:Polymyxin B; D013755:Tetradecanoylphorbol Acetate