A rapid assay for immunoglobulin in hybridoma supernatants.
A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods; D006825:Hybridomas / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007158:Immunologic Techniques; D051379:Mice; D013205:Staphylococcal Protein A / Q000378:metabolism
D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A