Excretion of neutral alpha-glucosidase, determined with a continuous assay, and of acid alpha-glucosidase in the urine of human reference subjects.
The catalytic activities of neutral and acid alpha-glucosidase were selectively determined in human urine. Urinary excretion of neutral and acid alpha-glucosidase in reference subjects was found to be in the range 1.61 to 20.36 microkat/mol creatinine and 7.47 to 33.60 microkat/mol creatinine, respectively. Urinary excretion of both enzymes was not related to sex, age or diuresis. A continuous assay was introduced to improve the determination of neutral alpha-glucosidase.
D000367:Age Factors; D001322:Autoanalysis; D003404:Creatinine / Q000652:urine; D005260:Female; D005959:Glucosidases / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D012016:Reference Values; D012737:Sex Factors; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D000520:alpha-Glucosidases / Q000652:urine*
D003404:Creatinine; D005959:Glucosidases; D000520:alpha-Glucosidases