The enzyme-linked immunosorbent assay of meningococcal and some related Escherichia coli polysaccharides.
A non-competitive enzyme-linked immunosorbent assay (ELISA) system has been developed for the quantitative, sensitive and specific determination of meningococcal polysaccharides. A prerequisite, however, is the correspondence of the O-acetyl content and the molecular size of the polysaccharides in the samples and the reference preparation. If these characteristics differ, special precautions in the use of antibodies to both the O-acetylated and the non-O-acetylated variants have to be taken, including the testing of the sample in at no less than three dilutions.
D000942:Antigens, Bacterial / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D007124:Immunoenzyme Techniques; D009343:Neisseria / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013045:Species Specificity
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial