Biochemical and cellular identification of HLA-Bw44 subtypes.
Biochemical analysis of HLA-Bw44 antigens by two-dimensional gelelectrophoresis and one-dimensional isoelectric focusing (IEF) from serologically heterozygous and homozygous donors allows the identification of two distinct types of HLA-Bw44 molecules, designated as Bw44-I and Bw44-II. These results are in concordance with the data obtained by CML-typing where at least two types of Bw44 target cells can be distinguished clearly as well. The antigen of type I has a more acidic isoelectric point (IEP) than that of type II. The difference in IEP are not due to differences in sialic acid content.
D005260:Female; D006680:HLA Antigens / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015235:HLA-B Antigens; D059948:HLA-B44 Antigen; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D008297:Male; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D006680:HLA Antigens; D015235:HLA-B Antigens; D059948:HLA-B44 Antigen