A micronucleus method for detection of meiotic micronuclei in male germ cells of mammals.
A new rapid micronucleus method is presented for the detection of chromosomal damage induced in spermatocyte stages of mammals. Analysis of micronuclei is done in early spermatids that have been isolated from testis tubules in a special testis isolation medium supplemented with enzymes (collagenase, trypsin and DNAase).
D000818:Animals; D003411:Arvicolinae; D002467:Cell Nucleus / Q000502:physiology*; D002869:Chromosome Aberrations; D008253:Macaca mulatta; D008297:Male; D008540:Meiosis / Q000528:radiation effects*; D051379:Mice; D008815:Mice, Inbred Strains; D009152:Mutagenicity Tests; D009154:Mutation; D051381:Rats; D011919:Rats, Inbred Strains; D013087:Spermatids / Q000528:radiation effects*; D013090:Spermatocytes / Q000528:radiation effects*; D013094:Spermatozoa / Q000528:radiation effects*