title: Effects of insulin and glucagon on amino acid transport into the liver and opercular muscle of the eel in vitro.
abstract: Effects of insulin and glucagon on amino acid transport in vitro into liver slices and the opercular muscle of the eel were studied using a labeled nonmetabolizable amino acid analog, alpha-[1-14C]aminoisobutyric acid (AIB). Addition of insulin (0.1 IU/ml) to the medium increased the radioactivity in the deproteinized fractions of both the liver slices and opercular muscle, indicating an accelerated movement of this amino acid analog into these tissues. Glucagon (5 micrograms/ml) also enhanced the entry of [14C]AIB into the liver slices; however, treatment of opercular muscle with glucagon did not alter the radioactivity in the deproteinized fraction. These findings clearly indicate that the entry of amino acids into eel tissues independent of protein synthesis can be altered by insulin and glucagon.
mesh_terms: D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D046508:Culture Techniques; D004524:Eels / Q000378:metabolism*; D005934:Glucagon / Q000502:physiology*; D007328:Insulin / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism
chemical_list: D000621:Aminoisobutyric Acids; D007328:Insulin; D005934:Glucagon