Ca2+-independent, protein-mediated fusion of chromaffin granule ghosts with liposomes.
We have investigated the interaction between isolated membrane vesicles from chromaffin granules and large unilamellar phospholipid vesicles (liposomes). Mixing of membrane lipids has been monitored continuously, utilizing the fluorescence resonance energy transfer assay described by Struck et al. ((1982) Biochemistry 20, 4093-4099). To demonstrate coalescence of the internal vesicle volumes the transfer of colloidal gold from the liposomes to the interior of the granule membrane vesicles has been examined. Efficient fusion of the liposomes with the granule membranes was observed. Significant fusion occurred in the absence of Ca2+, although the extent of interaction was enhanced in its presence. The sensitivity of the interaction to pretreatment of the granule membranes with trypsin showed the fusion reaction to be a protein-mediated process.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002788:Cholesterol Esters / Q000378:metabolism; D002837:Chromaffin Granules / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D004735:Energy Transfer; D005453:Fluorescence; D006048:Gold Colloid, Radioactive / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism
D002788:Cholesterol Esters; D006048:Gold Colloid, Radioactive; D008081:Liposomes; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; C014409:cholesteryl oleate; C042077:N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine; D002118:Calcium