title: Survey of plant inhibitors of polymorphonuclear leukocyte elastase, pancreatic elastase, cathepsin G, cathepsin B, Hageman factor fragments, and other serine proteinases.
abstract: Various flower bulbs and vegetable and legume seeds were tested for inhibitors of polymorphonuclear leukocyte elastase, pancreatic elastase, cathepsin G, cathepsin B, trypsin, alpha-chymotrypsin, Hageman factor fragments, plasma kallikrein, and plasmin. Calla bulbs contained a 33,000 dalton polymorphonuclear leukocyte elastase inhibitor and a 4,000 dalton cathepsin G inhibitor. Seeds of some members in the Cruciferae family, such as radish and broccoli, were found to contain one or more 2,500-4,000 dalton inhibitors which inhibited cathepsin G, trypsin, Hageman factor fragments, and plasmin, but not plasma kallikrein. These seeds also contained a 1,000 dalton cathepsin B inhibitor. The above inhibitors were probably polypeptides which inhibited proteinases by making an enzyme-inhibitor complex, with the exception of the cathepsin B inhibitor. These newly found inhibitors with their characteristic profiles of inhibition should be useful in biochemical and pathophysiological studies on granulocyte proteinases and enzymes of the coagulation and fibrinolytic pathways.
mesh_terms: D002401:Cathepsin B; D056649:Cathepsin G; D002403:Cathepsins / Q000037:antagonists & inhibitors; D005174:Factor XII / Q000037:antagonists & inhibitors; D015956:Factor XIIa; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010446:Peptide Fragments / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D013045:Species Specificity
chemical_list: D010446:Peptide Fragments; D010936:Plant Extracts; D011480:Protease Inhibitors; D005174:Factor XII; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D015956:Factor XIIa; D002401:Cathepsin B