[In vitro determination of the biological activity of the electrophoretic fractions of embryonic tissue extracts].
The possibility of direct combining the protein fractions after electrophoresis in polyacrylamide gel (PAAG) with the ectoderm of avian embryos in vitro was shown. The extract of eye vesicle of 30-33 hrs chick embryos were subjected to electrophoresis in 20% PAAG in capillaries (diameter 300 mu). The columns of PAAG were cut in equal parts and these  pieces with protein fractions were combined with the trunk ectoderm of 2 days old chick embryos. The preliminary analysis has shown that some fractions of the eye vesicle exert a selective neutralizing effect on the trunk ectoderm (formation of neural tubes). The PAAG itself had no toxic or inducing effect on the trunk ectoderm cells. The lens-inducing effect of the eye vesicle fractions was not observed.
D000818:Animals; D002642:Chick Embryo; D004475:Ectoderm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005123:Eye / Q000196:embryology*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D014020:Tissue Extracts / Q000032:analysis*
D014020:Tissue Extracts
Opredeleneie in vitro biologicheskoĭ aktivnosti élektroforeticheskikh fraktsiĭ ékstraktov iz émbrional'nykh tkaneĭ.