title: Toxicity of activated oxygen: lack of dependence on membrane unsaturated fatty acid composition.
abstract: Membrane unsaturated fatty acid oxidation has been suggested as a mechanism of toxicity for a variety of activated oxygen species. We have tested this hypothesis by manipulating the fatty acid composition of an Escherichia coli mutant that is unable to synthesize unsaturated fatty acids. To provide a wide range of susceptibility to membrane oxidation we have replaced the naturally occurring monoenoic acyl chains with cyclopropanes to greatly reduce the unsaturation level and with linoleate to increase the membrane unsaturation. These cultures were treated with ozone, hydrogen peroxide, singlet oxygen and paraquat. In no case was there substantial protection from toxicity afforded by cyclopropanes nor was there enhancement of toxicity to cells with the polyunsaturated membranes. We suggest, therefore, that oxidation of membrane unsaturated fatty acids is not an essential component of the toxicity to E. coli of active oxygen species.
mesh_terms: D004926:Escherichia coli / Q000187:drug effects*; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000633:toxicity; D008563:Membrane Lipids / Q000032:analysis*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity; D010269:Paraquat / Q000633:toxicity
chemical_list: D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D010126:Ozone; D006861:Hydrogen Peroxide; D010269:Paraquat; D010100:Oxygen