Kinetic and structural relationships of transition monomeric and oligomeric carboxyl- and choline-esterases.
The kinetic and structural relationships of eight electrophoretically pure mammalian serum and liver serine carboxylesterases (CE) and cholinesterases (ChE) have been studied. Eight CE's and ChE's, which were fully resolved but only partially purified, provided additional information. Five of the electrophoretically pure esterases were monomeric, and of these, four belonged to a new and widely distributed class. These four monomeric esterases hydrolyzed choline esters, but at widely differing rates. Thus two were termed monomeric butyrylcholinesterases, mBuChE I and II, and two were monomeric CE's (mCE). The rabbit liver mCE was not a subunit of the oligomeric CE (oCE), although the oCE also hydrolyzed choline esters at a very low rate. The complex kinetics of the mCE's, mBuChE's, oCE's, and of the oligomeric BuChE's of horse and human serum could be interpreted according to a single reaction scheme involving an allosteric site and the equation derived from it. Thus activation and inhibition at high substrate concentrations, together with sigmoidal activity versus substrate concentration plots, all of which characterize the reactions of these esterases, could be interpreted by a single scheme and equation. Structural and kinetic comparisons showed a progressive transition of properties from the oCE's through the mCE's to the oBuChE's. One of the purified mCE's was from horse serum, and it exhibited physical and kinetic properties unlike those of the liver mCE's or oCE's.
D000495:Allosteric Site; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D002802:Cholinesterases / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004950:Esterases / Q000037:antagonists & inhibitors; D006736:Horses; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D011817:Rabbits
D046911:Macromolecular Substances; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; D002091:Butyrylcholinesterase; D002802:Cholinesterases