Effect of serum from mice treated with lipopolysaccharide on cycling of CFUs in vitro.
Serum of lipopolysaccharide(LPS)-treated LPS-high-responder C3H/He mice was shown to increase survival of low-responder C3H/HeJ CFUs in an otherwise serum-free suspension culture by initiating cell cycling. Post-LPS serum of low-responder mice and serum of phosphate-buffered-saline-injected high-responder mice was significantly less effective in this respect. Since prolonged maintenance of CFUs was also found when cell suspensions highly enriched for stem cells were used, it seems unlikely that assessory cells mediated the effect of the post-LPS serum activity on CFUs maintenance. The serum activity did not enhance the stimulatory effect of saturating levels of highly purified stem-cell-activating factor (SAF) on CFUs maintenance in vitro. Upon injection of post-LPS serum from C3H/He mice a relatively small splenic CFUs accumulation in C3H/HeJ mice was observed.
D000818:Animals; D001769:Blood; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006918:Hydroxyurea / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D012475:Salmonella; D013154:Spleen / Q000166:cytology
D003115:Colony-Stimulating Factors; D008070:Lipopolysaccharides; D006918:Hydroxyurea