Ghost cells as sorption matrix for virus concentration from water.
Pig erythrocyte membranes were used as adsorbent material for the concentration of bacteriophage phi x-174, MS-2, and f2 from 5 ml of saline solution. The adsorption was carried out at pH 3.5, and the elution was carried out at pH 7.0. Compared with adsorption on 3% beef extract, bacteriophage adsorption on erythrocyte ghost cells yielded 93 to 100%, and elution was 92 to 100%, whereas beef extract organic flocculation yielded adsorption of 0 to 98.8% and elution of 61 to 86.6%. The same procedure but with poliovirus LSC-1 gave 100% adsorption and 91 to 129% elution.
D000327:Adsorption; D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification*; D004910:Erythrocyte Membrane; D008828:Microbiological Techniques; D017955:Poliovirus / Q000302:isolation & purification*; D013552:Swine; D014871:Water Microbiology