[Tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a protein in human lymphocytes binding satellite DNA].
Interactions between a satellite DNA fragment (SH3, 1.8 kb) cloned in pBR325 plasmid and DEAE-protein fractions from human lymphocytes treated with phorbol-12-myristate-13-acetate (TPA) have been studied by filtration technique through nitrocellulose filters. The 0.3 M fraction was found to have a protein which binds SH3 DNA specifically. Cytogenetic studies have shown an increased frequency of chromosome endoreduplications which may be due to the binding of TPA-induced protein to centromeres.
D001435:Bacteriophages / Q000235:genetics; D001798:Blood Proteins / Q000096:biosynthesis*; D002273:Carcinogens; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004276:DNA, Satellite / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D010704:Phorbols / Q000494:pharmacology*; D010957:Plasmids; D011485:Protein Binding / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D001798:Blood Proteins; D002273:Carcinogens; D004276:DNA, Satellite; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate
Opukholevyĭ promotor 12-O-tetradekanoilforbol-13-atsetat (TFA) indutsiruet v limfotsitakh cheloveka belok, sviazyvaiushchiĭ satellitnuiu DNK.