Culture of purified rat astrocytes in serum-free medium supplemented with mitogen.
We have obtained a highly purified astrocyte population in cultures originating from neonatal (2-5 days) rat cerebrum by use of the selection process provided by a serum-free chemically defined medium (DM). The addition of a glial growth factor isolated from bovine pituitary glands to DM induced in these astrocyte cultures both a stimulation of astrocytic proliferation and a morphological transformation of the astrocytes from flat fibroblastic form to multipolar stellate form.
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D001921:Brain / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D051381:Rats
D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins