Rotavirus infection of young children in two districts of Kenya from 1982 to 1983 as analyzed by electrophoresis of genomic RNA.
Employing techniques of polyacrylamide gel electrophoresis of viral RNA segments, we studied rotavirus strains and their relative contributions to rotavirus gastroenteritis epidemics in two major districts of Kenya. From early 1982 to the middle of 1983, 18 representative electropherotypes, including 6 short strains, were detected in 30 rotavirus specimens obtained from Nairobi, whereas 16, including 3 short strains, were detected in 70 virus specimens from coastal areas. With the exception of one strain, there were no identical electropherotypes between the two groups of rotaviruses obtained from these different districts. A change in predominant electropherotypes was observed in Mombasa in early 1983, and subsequently, newly occurring strains were detected in a small town along the coast when an apparent increase in gastroenteritis was observed in the district.
D002675:Child, Preschool; D004196:Disease Outbreaks / Q000453:epidemiology; D004591:Electrophoresis, Polyacrylamide Gel; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D007630:Kenya; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D012621:Seasons
D012367:RNA, Viral