[Use of agarose for isolating influenza virus proteins retaining a high immunogenicity].
A method of influenza virus separation in agarose was modified. The possibility of using agarose of the national make and Difco agar was established. RNP and M proteins were isolated. Highly active anti-M and anti-RNP sera were prepared which work well in the ELISA and RIA.
D000818:Animals; D002642:Chick Embryo; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D011863:Radioimmunoassay; D012685:Sepharose / Q000494:pharmacology*; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*; D014771:Virion / Q000276:immunology / Q000302:isolation & purification
D014764:Viral Proteins; D012685:Sepharose
Ispol'zovanie agarozy dlia izoliatsii belkov virusa grippa, sokhranivshikh vysokuiu immunogennost'.