Freeze substitution as a means of preserving meningeal vessel morphology.
This study compares freeze substitution with formaldehyde fixation as a means of preserving meningeal vessel morphometry. The diameters of vessels fixed by freeze substitution correlated very well with the in vivo diameters of the same vessels (correlation coefficient 0.9766). On the other hand, diameters of vessels perfusion fixed with 10% buffered formalin displayed poor correlation with in vivo diameters of the same vessels (correlation coefficient 0.1594).
D000818:Animals; D005615:Freezing; D006652:Histological Techniques; D008297:Male; D008576:Meningeal Arteries / Q000033:anatomy & histology*; D051381:Rats; D011919:Rats, Inbred Strains