Development of an in vitro assay for the survival of cells suspended from BA1112 rat sarcomas.
A method has been developed for the in vitro assay of the survival of BA1112 rat sarcoma cells following treatment in vivo. The BA1112 tumor was not adapted for growth in culture and was maintained by serial transplantation in vivo. The assay used a feeder layer of heavily irradiated cells to eliminate any changes in plating efficiency caused by the large number of dying cells which must be plated when assaying cell survival after intensive treatments. The plating efficiency of the tumor cells was not affected significantly by the age of the feeder layer (between 1 and 4 days), the density at which the experimental cells were plated or the interval between preparation of the cell suspension and plating (up to 4 hr). Using the in vitro assay a survival curve was determined for cells from tumors irradiated in air-breathing animals. This curve was similar to that determined previously for BA1112 tumor cells using an in vivo assay to measure cell survival.
D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D003584:Cytological Techniques; D051381:Rats; D012208:Rhabdomyosarcoma / Q000473:pathology*; D012513:Sarcoma, Experimental / Q000473:pathology; D013997:Time Factors