Use of a murine monoclonal antibody which binds to malignant keratinocytes to detect tumor cells in microscopically controlled surgery.
A new murine monoclonal antibody, termed VM-1, stains basal cell carcinoma cells and at least some squamous cell carcinoma cells. This antibody was used to visualize the tumor margins during microscopically controlled surgery using immunofluorescence (IF) and immunoperoxidase (IP) technics. Our experiments demonstrate that VM-1 is helpful in detecting small islands of tumor cells in the tissue at the margins of skin cancers. Thus, tissue staining with this antibody should improve the accuracy of microscopically controlled excision, and hopefully will decrease the rate of tumor recurrence after surgery.
D000818:Animals; D000911:Antibodies, Monoclonal; D002280:Basal Cell Carcinoma / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D008722:Methods; D051379:Mice; D008867:Microtomy; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery
D000911:Antibodies, Monoclonal