Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor.
Recombinant DNA techniques allow rapid construction and characterization of tools for genetic dissection of essential genes. In this report, we describe construction of a set of deletions in rpoD, the gene which encodes the sigma subunit of E. coli RNA polymerase. We used this deletion set to identify a region of the sigma polypeptide which is dispensable for its function.
D000595:Amino Acid Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005818:Genetic Engineering; D010957:Plasmids; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D012808:Sigma Factor; D014157:Transcription Factors