title: Enterobacterial common antigen-tetanus toxoid conjugate as immunogen.
abstract: The methods of limited periodate oxidation and reductive amination were used to obtain covalently linked enterobacterial common antigen (ECA) with tetanus toxoid. This procedure is simple and gives a good yield of the conjugate with high ECA content (molecular ratio of ECA to tetanus toxoid, 6:1). The ECA-tetanus toxoid conjugate is immunogenic in rabbits, in contrast to free ECA or a mixture of ECA with proteins. This conjugate produces high levels of ECA-specific immunoglobulin G antibodies, which can be used as a standard serum.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D004755:Enterobacteriaceae / Q000276:immunology*; D007114:Immunization; D007158:Immunologic Techniques; D011817:Rabbits; D013745:Tetanus Toxoid / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D013745:Tetanus Toxoid; C017286:enterobacterial common antigen