[Dependence of the efficiency of the fractionation of summary E. coli cell membranes on the method of their disintegration].
The efficiency of fractionation in the sucrose density gradient of E. coli cell membranes obtained after cell disintegration by ultrasonic, ballistic and extrusion methods was measured. The purity of individual membrane fractions was estimated by electron microscopy and polyacrylamide gel electrophoresis. The application of ballistic disintegration and solid state extrusion did not separate inner and outer membranes. Cell disintegration by means of ultrasonic treatment and liquid state extrusion allowed reproducible separation of membranes of the two types with a sufficiently high degree of purity.
D001426:Bacterial Proteins / Q000032:analysis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000166:cytology*; D008565:Membrane Proteins / Q000032:analysis; D008722:Methods; D008854:Microscopy, Electron; D014465:Ultrasonics
D001426:Bacterial Proteins; D008565:Membrane Proteins
Zavisimost' éffektivnosti fraktsionirovaniia summarnykh membran kletok E. coli ot sposoba ikh dezintegratsii.