Interaction between glucose and dehydroascorbate transport in human neutrophils and fibroblasts.
Dehydroascorbate (DHA) competitively inhibits the uptake of 2-deoxy-D-glucose and of 3-O-methyl-D-glucose by human blood neutrophils; 2-deoxyglucose and 3-O-methylglucose competitively inhibit dehydroascorbate uptake. Ratios of Km to Kl are near unity for each of these ligands. Exposure of cultured human fibroblasts to insulin enhances cytochalasin B-inhibitable uptake of DHA and of 2-deoxyglucose, both to the same degree. These observations suggest that DHA transport is mediated by glucose transport systems in human neutrophils and fibroblasts.
D019325:3-O-Methylglucose; D001205:Ascorbic Acid / Q000031:analogs & derivatives*; D001667:Binding, Competitive; D003571:Cytochalasin B / Q000494:pharmacology; D003683:Dehydroascorbic Acid / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*
D007328:Insulin; D008757:Methylglucosides; D019325:3-O-Methylglucose; D003571:Cytochalasin B; D003847:Deoxyglucose; D005947:Glucose; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid