A high molecular weight precursor of luteinizing hormone releasing hormone from rat hypothalamus.
A poly A+ enriched fraction of total RNA extracted from rat hypothalamus was translated in a messenger RNA-dependent cell free system from rabbit reticulocyte lysate supplemented with 3H-leucine. The translation products were immunoprecipitated using a specific antiserum to luteinizing hormone releasing hormone (LHRH). Following sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography of the immunoprecipitates, a single immunoreactive polypeptide was detected with an apparent molecular weight of 28,000. This is approximately twenty times larger than the active peptide, and thus suggests the presence of a high molecular weight precursor for the decapeptide, LHRH.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis*; D007031:Hypothalamus / Q000032:analysis*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011498:Protein Precursors; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone