Demonstration of an IgG-Fc receptor in ratcytomegalovirus infected cells.
Infection of rat embryonic fibroblasts (REF) and R2 cells with rat cytomegalovirus (RCMV) resulted in the formation of cytoplasmic and membrane receptors for the Fc region of immunoglobulin G. The Fc receptors were demonstrated by the immunocytochemical techniques using indirect fluorescence and peroxidase technique at the light microscope level and using the protein A adsorbed to colloidal gold technique for demonstration at the electron microscope level. The production level of demonstration at the electron microscope level. The production level of the Fc receptor in the cell was depending on the input of virus. Experiments with ara C, cycloheximide and actinomycin D revealed that the formation of the Fc receptor was dependent on RNA, DNA and protein synthesis. Furthermore, results indicate that redistribution of the Fc receptor to form a cap, takes place within the first 60 minutes.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008854:Microscopy, Electron; D051381:Rats; D011961:Receptors, Fc / Q000032:analysis*; D017452:Receptors, IgG
D011961:Receptors, Fc; D017452:Receptors, IgG