Semi-automated continuous-flow enzyme immunoassay for antiepileptic drugs in serum.
We have developed a semi-automated method for measuring five kinds of antiepileptic drugs in serum by successfully adapting commercial competitive-binding enzyme immunoassay kits (MARKIT; Dainippon) for use with a continuous-flow analyzer (Technicon AutoAnalyzer II equipped with a dialyzer). The free enzyme-labeled drug is automatically separated by a microfilter from the competitive immunoreaction mixture between labeled and unlabeled drug for anti-drug immunoglobulin coupled to bacterial cell walls. The concentrations of the antiepileptic drugs in serum samples can be determined by automated measurement of enzyme activity of the enzyme-labeled drugs. Results of the semi-automated method correlated well with those obtained by manual enzyme immunoassay, gas-liquid chromatography, and "high-pressure" liquid chromatography. The correlation coefficients were all greater than 0.95, showing the practicality of this method for therapeutic monitoring of antiepileptic drugs.
D000927:Anticonvulsants / Q000097:blood* / Q000302:isolation & purification; D001322:Autoanalysis / Q000295:instrumentation; D002220:Carbamazepine / Q000097:blood; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007124:Immunoenzyme Techniques; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011933:Reagent Kits, Diagnostic
D000927:Anticonvulsants; D011933:Reagent Kits, Diagnostic; D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital