Synthesis of a double-stranded cDNA transcript of the killer toxin-coding region of the yeast M1 double-stranded RNA.
Reverse transcription of methylmercuryhydroxide-treated M1 double-stranded RNA of yeast produces several discrete single-stranded and double-stranded cDNA's from oligo (dT)12-18 primer. S1 nuclease analysis shows that the longest transcript of the 1.8 kb template, a 2.2 kb molecule, is a 1.1 kb duplex terminated at one end by a hairpin-like structure. The 1.1 kb ds cDNA contains a complete copy of the M1-1, toxin-coding, sequence of M1 double-stranded RNA, and its synthesis is primed from oligo (dT)12-18 that anneals to a sequence within the internal, AU-rich, region of the template.
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000235:genetics*; D005796:Genes; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012330:RNA, Double-Stranded / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013698:Templates, Genetic; D014158:Transcription, Genetic
D005656:Fungal Proteins; C527157:KHR1 protein, S cerevisiae; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D012330:RNA, Double-Stranded; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA