Solubilization of yeast plasma membranes and mitochondria by different types of non-denaturing detergents.
A comparative study of the solubilization of yeast plasma membranes and mitochondria by different types of non-denaturing detergents has been performed. Zwittergent-14 (3-[tetradecyldimethylammonio]-1-propanesulfonate) at low concentrations (3-4 mM) produced maximum solubilization of both membranes. However, this detergent may inactivate enzymes at high concentrations. Taurodeoxycholate (in the presence of salt) and Triton X-100 were also effective in mitochondria but not in the plasma membranes. Octylglucoside only solubilized these membranes at very high concentrations (20 mM). CHAPS (3-[cholamidopropyldimethylammonio]-1-propanesulfonate) only achieved partial solubilization even at high concentrations. Our results suggest that Zwittergent-14 at low concentrations is one of the most powerful detergents for the general solubilization of native membrane proteins.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure*; D003902:Detergents; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008928:Mitochondria / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D012995:Solubility; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents
D003902:Detergents; D013501:Surface-Active Agents