title: Localization of enkephalin-like immunoreactivity in acetylcholinesterase-positive cells in the guinea-pig lateral superior olivary complex that project to the cochlea.
abstract: Olivocochlear fibers have been demonstrated to have acetylcholinesterase-positive staining both in brainstem and cochlea. Olivocochlear fibres in the cochlea have also been determined to contain enkephalin-like immunoreactivity. In this study, we first determined the source of olivocochlear fibers in the guinea-pig using horseradish peroxidase and wheat germ agglutinin in retrograde transport studies. These cells were then examined for enkephalin-like immunoreactivity followed by acetylcholinesterase staining on the same sections to determine which cells and fibers showed staining for both. It was found that cells in the guinea-pig lateral superior olive that project to the cochlea have both enkephalin-like immunoreactivity staining and acetylcholinesterase-positive staining. Cells in other areas giving rise to olivocochlear fibers showed only acetylcholinesterase staining. These results suggest that there is co-localization of enkephalin and acetylcholine in a population of olivocochlear cells and fibers.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D003051:Cochlea / Q000294:innervation*; D003056:Cochlear Nerve / Q000201:enzymology*; D004745:Enkephalins / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D009474:Neurons / Q000201:enzymology; D009847:Olivary Nucleus / Q000201:enzymology*
chemical_list: D004745:Enkephalins; D000110:Acetylcholinesterase