[Effect of low temperature shock and extracellular ice formation on the external and cytoplasmic membranes of Escherichia coli cells].
The effect of low temperature shock and extracellular ice formation on the properties of the outer and cytoplasmic membranes of E. coli K-12 cells was studied. The shock released intracellular K+ ions and inhibited the respiratory activity of the cells, suggesting that the cytoplasmic membrane was damaged. Crystallization of extracellular water caused additional disorders of the cytoplasmic membrane. Damages of the outer membrane detected by permeability for ethidium bromide appeared only after extracellular ice formation. The outer membrane of E. coli was not susceptible to a cold shock.
D002462:Cell Membrane; D003080:Cold Temperature; D004926:Escherichia coli / Q000648:ultrastructure*; D007053:Ice; D007425:Intracellular Membranes
D007053:Ice
Deĭstvie shoka, vyzvannogo nizkoĭ temperaturoĭ, i vnekletochnogo obrazovaniia l'da na vneshniuiu i tsitoplazmaticheskuiu membrany kletok Escherichia coli.