[Identification and analysis of hydrogenated cyclic monomeric acids in the hepatic lipids of rats ingesting heated linseed oil].
In a short-term (15 days) experiment, weanling Wistar rats were given diets at weaning containing 10% of different linseed oils: fresh linseed oil (lot F), linseed oil heat polymerized at 275 degrees C for 12 h under nitrogen (lot T) and linseed oil oxidized in the presence of air at 200 degrees C for 100 h (lot O). We analyzed the fatty acids of total liver lipids and assayed the hydrogenated cyclic monomeric acids and identified them by using a combination of gas-liquid chromatography and mass spectrometry. Compared to lot F animals, those of lots T and O showed considerable hepatic hypertrophy but total liver lipids were not increased. However, the usual fatty acid composition of these lipids was modified: the level of several monounsaturated (16: 1, 18: 1) and polyunsaturated (mainly 20: 4n - 6) fatty acids was increased in lots T and O, which could result in increased activity of the enzymatic systems (desaturases) biosynthesizing them. Moreover, cyclic monomeric acids from heated oils were deposited at a 10 times higher concentration in the liver of lot T animals than in that of lot O animals. The significance of these changes has been discussed.
D000818:Animals; D001835:Body Weight; D005227:Fatty Acids / Q000032:analysis*; D006865:Hydrogenation; D008043:Linseed Oil / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D014886:Weaning
D005227:Fatty Acids; D008055:Lipids; D008043:Linseed Oil
Identification et dosage des acides monomères à structure cyclique hydrogénés dans les lipides hépatiques du rat ayant ingéré de l'huile de lin chauffée.