Analysis of cellular events involved in T cell stimulation by concanavalin A as a function of cell concentration.
To investigate cell-to-cell interactions in T cell stimulation by concanavalin A (Con A), three distinct events involved in the stimulation--production of interleukin 2 (IL 2), emergence of IL 2 receptors, and responses of cells bearing IL 2 receptors to IL 2--have been separately determined as functions of the cell concentration. Results obtained at concentrations as low as 5 X 10(4) cells/ml showed that both the emergence of IL 2 receptors and the induction of DNA synthesis by IL 2 of lymphocytes bearing IL 2 receptors occurred in a manner independent of the concentration of cells, whereas the production of IL 2 depended greatly on the cell density. These results suggest that the production of IL 2 requires cell-to-cell interactions, but the other two events do not.
D000818:Animals; D002450:Cell Communication; D002452:Cell Count; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D007163:Immunosorbent Techniques; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008811:Mice, Inbred DBA; D011971:Receptors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*
D007376:Interleukin-2; D011971:Receptors, Immunologic; D003208:Concanavalin A; D004247:DNA