Generation of an interleukin-I-like lymphocyte-stimulating factor at inflammatory sites: correlation with the infiltration of polymorphonuclear leucocytes.
Using a mouse thymocyte co-stimulation assay, we demonstrated thymocyte-stimulating activity in murine peritoneal fluid obtained from the early stage (3 to 9 h) of casein-induced inflammation. This early inflammatory stage coincided with the time at which an influx of polymorphonuclear leucocytes (PMN) into the inflamed site was observed. Similar thymocyte-stimulating activity was produced in vitro by PMN purified from 4-h peritoneal exudate but not by purified PMN obtained at a later stage (24 h) of the inflammation. The inflammatory factor was interleukin (IL)-I-like; it was devoid of IL-2 activity when tested with IL-2-dependent cells. It could stimulate murine thymocytes to produce IL-2. On a Sephadex G-75 column, the factor was eluted between the molecular sizes of 10 000 and 30 000; its peak activity was at 21 000. The factor mainly consisted of two (pI 6.5 and pI 5.0) iso-electrophoretically different factors.
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism / Q000473:pathology; D002850:Chromatography, Gel; D005260:Female; D066298:In Vitro Techniques; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D007375:Interleukin-1 / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology; D013997:Time Factors
D007375:Interleukin-1