The primary structure of rat liver ribosomal protein L37. Homology with yeast and bacterial ribosomal proteins.
The covalent structure of the rat liver 60 S ribosomal subunit protein L37 was determined. Twenty-four tryptic peptides were purified and the sequence of each was established; they accounted for all 111 residues of L37. The sequence of the first 30 residues of L37, obtained previously by automated Edman degradation of the intact protein, provided the alignment of the first 9 tryptic peptides. Three peptides (CN1, CN2, and CN3) were produced by cleavage of protein L37 with cyanogen bromide. The sequence of CN1 (65 residues) was established from the sequence of secondary peptides resulting from cleavage with trypsin and chymotrypsin. The sequence of CN1 in turn served to order tryptic peptides 1 through 14. The sequence of CN2 (15 residues) was determined entirely by a micromanual procedure and allowed the alignment of tryptic peptides 14 through 18. The sequence of the NH2-terminal 28 amino acids of CN3 (31 residues) was determined; in addition the complete sequences of the secondary tryptic and chymotryptic peptides were done. The sequence of CN3 provided the order of tryptic peptides 18 through 24. Thus the sequence of the three cyanogen bromide peptides also accounted for the 111 residues of protein L37. The carboxyl-terminal amino acids were identified after carboxypeptidase A treatment. There is a disulfide bridge between half-cystinyl residues at positions 40 and 69. Rat liver ribosomal protein L37 is homologous with yeast YP55 and with Escherichia coli L34. Moreover, there is a segment of 17 residues in rat L37 that occurs, albeit with modifications, in yeast YP55 and in E. coli S4, L20, and L34.
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000032:analysis*; D003488:Cyanogen Bromide; D008099:Liver / Q000032:analysis*; D051381:Rats; D012269:Ribosomal Proteins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D029701:Saccharomyces cerevisiae Proteins; D014357:Trypsin / Q000378:metabolism
C115278:RPL43a protein, S cerevisiae; D012269:Ribosomal Proteins; C039161:Rpl37 protein, rat; D029701:Saccharomyces cerevisiae Proteins; D014357:Trypsin; D003488:Cyanogen Bromide