A simple and rapid method for quantification of hapten specific antibodies.
A sensitive and simple method for quantitation of antibodies against small molecules is described using DNP-lysozyme as the enzyme conjugate. The anti-DNP antiserum was raised against DNP-bovine serum albumin conjugate. Anti-DNP antibody or its monovalent fragment (Fab) reduced the enzyme activity of DNP-lysozyme conjugate in a concentration-dependent manner. The inhibition of enzyme activity is a specific measure of the antibody and Fab content of the sample. The specificity of the reaction was assessed by reduction of antibody-induced inhibition by DNP-lysine. The ability of DNP-lysine to reduce the antibody-induced inhibition of DNP-lysozyme activity also makes possible a sensitive assay for DNP-lysine.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D004136:Dinitrobenzenes / Q000276:immunology; D006241:Haptens / Q000276:immunology; D007158:Immunologic Techniques; D011817:Rabbits
D000906:Antibodies; D004136:Dinitrobenzenes; D006241:Haptens