Cell-free translation of human renin mRNA.
The molecular weight of human renin precursor (preprorenin) was determined to be 43,000 by cell-free translation of its mRNA. Poly(A)+-containing RNA from human infarcted kidney was translated in a cell-free reticulocyte lysate system containing [35S] methionine. Immunoprecipitation with anti-renin antibody gave one major band with an apparent Mr = 43,000, as determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and fluorography. The specific band, which represents the prepro-form of human renal renin, was very similar to mouse submandibular gland preprorenin in its electrophoretic mobility.
D000818:Animals; D002474:Cell-Free System; D006801:Humans; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D012083:Renin