Release of platelet-activating factor (PAF)-like material from human lymphoid cell lines.
The release of platelet-activating factor (PAF)-like material was evaluated from eight B and T human leukemic cell lines and from three lymphoblastoid cell lines obtained after Epstein-Barr virus (EBV) transformation. All cell lines were stimulated with ionophore A23187, phytohemagglutinin (PHA), or both either in the presence of or without acetyl coenzyme A (AcCoA). After appropriate stimuli, all cell lines tested released PAF-like material, irrespective of the surface phenotype and of the level of differentiation. Some cell lines released PAF-like material after stimulation with A23187 (Molt-4, Jurkat, 1301, Raji, FM007), others only if AcCoA was added to A23187 (CCRF/CEM, HUT 78, Daudi, Nalm-1). In some cases, PHA was capable of enhancing the effect of A23187 (Molt-4, HUT 78, Daudi, IBW4) and finally, in a lymphoblastoid cell line (WT20), the release of PAF-like material was achieved only in the presence of both A23187 and PHA. These findings demonstrate for the first time that human lymphoid cells both of B- and of T-cell origin can release a PAF-like material after appropriate stimuli.
D001402:B-Lymphocytes / Q000378:metabolism*; D002051:Burkitt Lymphoma; D002460:Cell Line; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007938:Leukemia; D007945:Leukemia, Lymphoid; D008223:Lymphoma; D010972:Platelet Activating Factor / Q000032:analysis*; D013601:T-Lymphocytes / Q000378:metabolism*
D006680:HLA Antigens; D010972:Platelet Activating Factor