Standardized shake-off to synchronize cultured CHO cells.
A protocol to obtain CHO cells synchronized in mitosis is described. The synchronized population contained hardly any S-phase cells as judged by the incorporation of 3H-TdR. In these cells a very low frequency of chromatid-type aberrations compared with chromosome-type aberrations was induced by X-rays at 1.5 h after the synchronization.
D000818:Animals; D002460:Cell Line; D002842:Chromatids / Q000528:radiation effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D003584:Cytological Techniques; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008938:Mitosis; D010053:Ovary; D014965:X-Rays