Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector.
A chimaeric gene, consisting of the 5'-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.
D000123:Acetyltransferases / Q000235:genetics*; D001483:Base Sequence; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase; D002736:Chloroplasts / Q000201:enzymology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes / Q000528:radiation effects; D005822:Genetic Vectors; D008027:Light; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes; D012273:Ribulose-Bisphosphate Carboxylase