Membrane alterations in magnesium-deficiency-induced malignant T cell lymphoma.
Malignant T cell lymphoma cells induced in the thymus by chronic Mg deficiency showed a less ruffled surface in the scanning electron microscope, particularly after intraperitoneal transplantation. Their content of phosphatidylserine and phosphatidylinositol was increased. The content of phosphatidylcholine, cholesterol and the cholesterol/phospholipid ratio, and thus membrane microviscosity, were decreased. The activity of phospholipid methyltransferase I and II was reduced. In parallel to these alterations, 45Ca2+ influx and efflux as well as 45Ca2+ content in mitochondria, microsomes and cytosol of lymphoma cells were enhanced.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure*; D002784:Cholesterol / Q000032:analysis; D005260:Female; D008223:Lymphoma / Q000209:etiology*; D008275:Magnesium Deficiency / Q000150:complications*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D008780:Methyltransferases / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase; D050918:Phosphatidylethanolamine N-Methyltransferase; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes
D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase; D002118:Calcium