Immunocytochemically identified vasopressin neurons in culture show slow, calcium-dependent electrical responses.
From morphological characterization and intracellular recordings, monolayer cultures derived from fetal mouse hypothalami were found to include functionally differentiated peptide neurons, a number of which appear to contain vasopressin. These cells exhibited particular patterns of slow, calcium-dependent membrane depolarizations, resembling in their periodicity and duration the phasic activity of vasopressin neurons recorded extracellularly in vivo.
D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D004594:Electrophysiology; D006651:Histocytochemistry; D007031:Hypothalamus / Q000032:analysis / Q000166:cytology*; D007158:Immunologic Techniques; D051379:Mice; D009474:Neurons / Q000032:analysis; D014667:Vasopressins / Q000032:analysis*
D014667:Vasopressins; D002118:Calcium