Determination of glutathione in biological material by high pressure liquid chromatography.
A rapid and selective determination of reduced glutathione in biological material is described, based on its conjugation with 1-chloro-2,4-dinitrobenzene. The reaction product has a UV absorption maximum at 340 nm and is analysed by reversed phase high pressure liquid chromatography. Linear calibration graphs were obtained in the concentration range between 50 microM and 2 mM glutathione in standard solutions and in biological material (rat liver and bacterial homogenates). The detection limit is about 2 microM glutathione when using 20 microliters injection samples.
D000111:Acetylcysteine / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003545:Cysteine / Q000032:analysis; D004137:Dinitrochlorobenzene; D004926:Escherichia coli / Q000032:analysis; D005978:Glutathione / Q000032:analysis*; D008099:Liver / Q000032:analysis; D051381:Rats
D004137:Dinitrochlorobenzene; D005978:Glutathione; D003545:Cysteine; D000111:Acetylcysteine