Reduced PLD repair ability in glutathione synthetase deficient human fibroblasts after UV irradiation.
Using a human cell strain deficient in glutathione synthetase and a related control, the role of glutathione in repair mechanisms has been investigated. UV light has been used in order to avoid the interaction between thiols and free radicals. When potentially lethal damage repair is completed, deficient cells in plateau phase exhibit smaller surviving fractions than do control cells. The ratio of surviving fractions in control/deficient cells is about 2 for the same radiation dose. These results indicate that thiols and especially GSH are involved in repair mechanisms.
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004260:DNA Repair; D005978:Glutathione / Q000378:metabolism / Q000502:physiology*; D005981:Glutathione Synthase / Q000172:deficiency; D006801:Humans; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014466:Ultraviolet Rays
D013438:Sulfhydryl Compounds; D005981:Glutathione Synthase; D005978:Glutathione