Influence of PMA on T-lymphocyte responses to mitogenic lymphokines.
Phorbol myristate acetate (PMA), a reagent frequently used in vitro to bypass macrophage function and to elicit cytokines, has a variety of effects on the behavior of T lymphocytes in tissue culture. For example, PMA in concentrations greater than 10(-10)M is highly comitogenic with phytohemagglutinin (PHA) for C3H/HeJ thymocytes, and significantly potentiates lymphokine-induced proliferative responses of long-term MLC cells, cloned cytolytic T cells, and interleukin-2 (IL-2)-dependent T-cell lines. Since these activated T-lymphocyte populations are used to detect IL-2, PMA can interfere with accurate assessment of IL-2 concentration by quantitative bioassay. Further, if greater than 10(-10) M PMA remains as a contaminant in PMA-induced lymphokine preparations, it can mediate lymphokine-like biologic activity, and, therefore, obscure interpretation of experiments involving lymphocyte-lymphokine interactions.
D000818:Animals; D002999:Clone Cells / Q000187:drug effects; D005260:Female; D007376:Interleukin-2 / Q000502:physiology*; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010704:Phorbols / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013347:Subcellular Fractions / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D007376:Interleukin-2; D037102:Lectins; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine