Rapid method for separation and purification of four isoenzymes of phosphodiesterase from Trimeresurus flavoviridis (Habu snake) venom.
Four isoenzymes of phosphodiesterase were identified in Trimeresurus flavoviridis venom. A rapid and highly reproducible column chromatographic procedure on CM-Sephadex C-25, QAE-Sephadex A-25 and Sephadex G-100 was developed for the purification of these isoenzymes with a yield of 83%. All four isoenzymes are metalloglycoproteins having negligible amounts of phosphomonoesterase activity.
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D003435:Crotalid Venoms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007527:Isoenzymes / Q000032:analysis; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000302:isolation & purification*; D012911:Snakes; D013997:Time Factors
D003435:Crotalid Venoms; D007527:Isoenzymes; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom