Antiretinoblastoma monoclonal antibodies.
Mouse-mouse somatic hybridization techniques were used to produce monoclonal antibodies directed towards antigen expressed on retinoblastoma-derived tissue culture cell lines. Four monoclonal antibodies were produced with varying avidity which were reactive against allogeneic retinoblastoma-associated antigens. Sensitivity and specificity was demonstrated using ELISA and chromium release assays. Immunofluorescent antibody studies suggest that these monoclonal antibodies may be useful diagnostically.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005134:Eye Neoplasms / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D012175:Retinoblastoma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007136:Immunoglobulins