title: Long-term cultures of human adult Schwann cells isolated from autopsy materials.
abstract: Enriched populations of adult human Schwann cells were obtained from trigeminal ganglia and roots of autopsy material. The cells, isolated by enzymatic procedure, were seeded on rat tail collagen-coated coverslips. Subcultures were established several weeks later, and secondary cells were grown on polylysine-coated coverslips and maintained in vitro for as long as 5 months. The Schwann cells in culture displayed the same light- and electron-microscopic features and arrangement as those cells observed in vivo. The addition of bovine pituitary glial growth factor in the medium induced a 3-5-fold increase in Schwann cell division.
mesh_terms: D000328:Adult; D001345:Autoradiography; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D020904:Glia Maturation Factor; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D012583:Schwann Cells / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology; D014276:Trigeminal Nerve / Q000166:cytology
chemical_list: D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins; D003561:Cytarabine; D013936:Thymidine