title: Isolation of immunogenic and suppressogenic glycoproteins from adenovirus type 12 hamster tumor cells.
abstract: Two types of glycoproteins were isolated from the membrane fraction of adenovirus type 12 (Ad12) hamster tumor cells by recovering detergent-solubilized glycoproteins using concanavalin A-affinity chromatography and gel filtration. One of the glycoproteins consisted of a polypeptide of 130,000 daltons (130K) with a pI value of 4.7-5.1, and the other consisted of a polypeptide of 18,500 daltons (18.5K) with a pI value of 6.3-6.6. The glycoproteins were immunologically different. The 18.5K glycoprotein induced in vivo resistance to tumor growth and anti-tumor cytotoxic T cells, while the 130K glycoprotein induced in vivo suppressor T cells which inhibited the activity of anti-tumor cytotoxic T cells.
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology; D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D006224:Cricetinae; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D009363:Neoplasm Proteins / Q000276:immunology* / Q000302:isolation & purification; D009374:Neoplasms, Experimental / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D006023:Glycoproteins; D009363:Neoplasm Proteins