title: Elastase, collagenase, and the biaxial elastic properties of dog carotid artery.
abstract: Segments of dog carotid artery were studied in vitro at four longitudinal extension ratios, lambda z = 1.2, 1.4, 1.6, and 1.8, in randomized order. At each length, the pressure was elevated in steps to 200 mmHg or until the vessels buckled. Vessels were studied under control conditions and after treatment with moderate doses of degradative enzymes: 80 U/ml elastase for 90 min or 640 U/ml collagenase for 120 min. These doses were selected, following pilot studies, to degrade vessels but not to destroy them. Treatment with elastase (n = 24) reduced both longitudinal and circumferential stresses at all vessel lengths. Circumferential stress was reduced at pressures greater than 15 mmHg, the magnitude of effect increasing with both longitudinal and circumferential deformations. Longitudinal stress was reduced by a constant amount, irrespective of vessel length. Treatment with collagenase (n = 24) reduced circumferential stress when the vessels were distended by at least 60 mmHg; it did not reduce longitudinal stress. These data suggest that in intact cylindrical segments of dog carotid artery, subjected to physiological levels of strain, elastin bears a portion of both circumferential and longitudinal loads, whereas collagen bears a portion of only circumferential loads.
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D004285:Dogs; D004548:Elasticity; D004549:Elastin / Q000502:physiology; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000494:pharmacology*; D013314:Stress, Mechanical
chemical_list: D003094:Collagen; D004549:Elastin; D010196:Pancreatic Elastase; D003012:Microbial Collagenase