Effect of insulin, thyroxine and oestradiol on growth of normal human diploid cells and human heteroploid HeLa cells.
Insulin (5 micrograms/ml), thyroxine (1 microgram/ml) and beta-oestradiol (0.1 microgram/ml), if added separately to the medium, did not significantly influence the growth of normal human diploid LEP 19 cells cultivated in Eagle's MEM medium with 1% foetal calf serum. Combined, they did not influence the growth of LEP 19 cells in serum-free MEM medium either. Under the same cultivation conditions, insulin caused 37%, thyroxine 24% and a mixture of insulin, thyroxine and beta-oestradiol 325% (without serum) or 15% (with 1% foetal calf serum) stimulation of the growth of human heteroploid HeLa cells. Isolated beta-oestradiol significantly inhibited the growth of HeLa cells.
D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured; D004171:Diploidy; D004958:Estradiol / Q000494:pharmacology*; D006367:HeLa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D009696:Nucleic Acids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology*
D007328:Insulin; D009696:Nucleic Acids; D011506:Proteins; D004958:Estradiol; D013974:Thyroxine