title: Recombination-induced suppression of cell division following P1-mediated generalized transduction in Klebsiella aerogenes.
abstract: Klebsiella aerogenes recombinants resulting from bacteriophage P1-mediated generalized transduction failed to increase in number for approximately six generations after transduction. Nevertheless these recombinants continued to grow and became sensitive to penicillin after a transient resistance, suggesting that the cells were growing as long, non-dividing filaments. When filamentous cells were isolated from transduced cultures by gradient centrifugation, recombinants were 1000-fold more frequent among the filaments than among the normal-sized cells. The suppression of cell-division lasted for six generations whether markers near the origin (gln, ilv) or terminus (his, trp) of chromosome replication were used, despite a 50-fold difference in transduction frequencies for these markers. The suppression of cell division was a host response to recombination rather than to P1 invasion since cells lysogenized by P1 in these same experiments showed only a short (two generation) suppression of cell division. We speculate that the suppression of cell-division is an SOS response triggered by the degraded DNA not incorporated in the final recombinant. We demonstrate that both the filamentation and the transient penicillin resistance of recombinant cells can be exploited to enrich greatly for recombinants, raising transduction frequencies to as high as 10(-3).
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D002455:Cell Division; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology; D011995:Recombination, Genetic; D014161:Transduction, Genetic
chemical_list: D010406:Penicillins