Indirect immunoperoxidase test for the diagnosis of paratuberculosis.
An indirect immunoperoxidase procedure for the diagnosis of paratuberculosis was described. Formalin-fixed ileocecal tissue containing large numbers of Mycobacterium paratuberculosis organisms was used as the source of antigen. Goat anti-bovine immunoglobulin G labeled with peroxidase was used as the conjugate in the test system. The method is relatively simple to do and may prove to be valuable as a routine screening test.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D007124:Immunoenzyme Techniques; D009161:Mycobacterium / Q000276:immunology*; D010283:Paratuberculosis / Q000175:diagnosis*
D000907:Antibodies, Bacterial