Insulin-like peptides in the lobster Homarus americanus. I. Insulin immunoreactivity.
Experiments were conducted to determine if insulin-like peptides are present in the lobster Homarus americanus. Peptides were found that bind specifically to bovine insulin antibodies in a modified vertebrate radioimmunoassay. Extracts of whole hepatopancreas, gut, and hemolymph contained insulin immunoreactivity (IRI) concentrations of 67.5, 14.0, and 11.0 ng, respectively, per 700-g lobster. No insulin immunoreactivity was detected in neurosecretory cells of the eyestalk. The highest immunoreactivity was measured in the hepatopancreas, in the same fractions of eluate which contained the highest immunoreactivity when a bovine insulin standard was passed through the same chromatographic column.
D000818:Animals; D002850:Chromatography, Gel; D006458:Hemolymph / Q000032:analysis; D007328:Insulin / Q000032:analysis*; D007422:Intestines / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D008121:Nephropidae / Q000378:metabolism*; D010179:Pancreas / Q000032:analysis; D011863:Radioimmunoassay; D014018:Tissue Distribution
D007328:Insulin