Purification of alpha 1-proteinase inhibitor by triazine dye affinity chromatography, ion-exchange chromatography and gel filtration on Fractogel TSK.
The purification of plasma proteins by affinity chromatography on triazine dye matrices can be optimised with regard to the triazine dye used as a group-specific ligand. A comparison by electrophoretic and immunological means of the results of affinity chromatography with human plasma on Fractogel TSK-Blue and Fractogel TSK-Red demonstrated that the Procion Red HE-3B-containing gel was able to adsorb more plasma constituents than the Cibacron Blue F3-GA gel. The preparation of alpha 1-proteinase inhibitor obtained after chromatography on Fractogel TSK-Red showed a higher degree of purity and could easily be further purified by ion-exchange chromatography on Fractogel TSK DEAE-650 and gel filtration on Fractogel TSK HW 55, without significant loss of biological activity.
D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D014227:Triazines; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification*
D014227:Triazines; D000515:alpha 1-Antitrypsin; C020795:procion red HE-3B; C038830:DEAE affigel blue