title: A nucleotide change in the anticodon of an Escherichia coli serine transfer RNA results in supD-amber suppression.
abstract: The tRNAs specified by the wild type and amber suppressor alleles of the Escherichia coli supD gene have been identified, and their primary structures determined. The sequences differ by a single nucleotide in the middle of the anticodon. A CUA anticodon allows the suppressor tRNA to read the UAG stop codon; the CGA anticodon in the minor serine tRNA species from which the suppressor is derived is specific for the serine codon UCG.
mesh_terms: D000483:Alleles; D000926:Anticodon / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D000926:Anticodon; D003062:Codon; D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer