The effects of Amicon and millipore membranes upon the lymphokine titres of culture supernatants.
Antigen- and Concanavalin A-induced culture supernatants have been reported to contain inhibitors of interleukin 2 (IL2) activity which necessitate concentration of samples over Amicon membranes prior to lymphokine assay. However, since we found this to be unnecessary, we have re-evaluated the effects of Amicon and millipore membranes upon the lymphokine titres of such supernatants. Our findings do not support the notion of an IL2 inhibitor, but indicate that lymphokine levels can be dramatically reduced as a consequence of millipore filtration.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005374:Filtration / Q000295:instrumentation*; D007376:Interleukin-2 / Q000037:antagonists & inhibitors; D008222:Lymphokines / Q000032:analysis*; D008567:Membranes, Artificial; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000032:analysis
D007376:Interleukin-2; D008222:Lymphokines; D008567:Membranes, Artificial; D003208:Concanavalin A