[Detection of humoral antibodies to genital organ antigens in sterile couples].
Sera from 150 infertile and 50 fertile couples were examined for circulating anti-genital organ antibodies by indirect immunofluorescence test on unfixed cryostat sections from various organs and methanolfixed smears of spermatozoa. -- Antibodies to spermatozoa showed an acrosomal, equatorial, postnuclear or tail immunofluorescence pattern. -- Antibodies to the ovary were directed to theca folliculi and to stromal antigens. Antibodies to the Fallopian tube reacted with the apical parts of the epithelial cells. Antibodies to endometrium and cervix were directed to glandular epithelium or mucosal stroma and antibodies to placenta reacted with trophoblast cells or amnion epithelium. Testicular antibodies were directed to tunica propria or the germinal epithelium. -- Antibodies to epididymis stained the mucous membrane epithelium, basal cells, the tunica propria or epididymal spermatozoa. Antibodies to the prostate and seminal vesicles reacted with glandular epithelium or stroma. -- Autoimmune mechanisms may play diverse roles in the pathogenesis of dysfunction of female and male genital organs.
D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology; D001666:Binding Sites, Antibody; D005260:Female; D005455:Fluorescent Antibody Technique; D005836:Genitalia, Female / Q000276:immunology*; D005837:Genitalia, Male / Q000276:immunology*; D006801:Humans; D007247:Infertility, Female / Q000276:immunology*; D007248:Infertility, Male / Q000276:immunology*; D008297:Male; D013094:Spermatozoa / Q000276:immunology
D001323:Autoantibodies; D001324:Autoantigens
Nachweis humoraler Antikörper gegen Genitalorganantigene bei sterilen Ehepaaren.