[Determination of the acetylcholinesterase activities of the amniotic fluid on a centrifugal analyzer].
Amniotic fluid cholinesterase (ChE) activity measurements are realized to detect pre-natal neural tube defects. Their determinations on centrifugal analyzer are described. Total ChE activity is assayed at 412 nm with dithiobis-(nitrobenzoic-acid). Acetylcholinesterase activity is measured in the presence of ChE inhibitor, 14 X 10(-6)M lysivane; our results about the inhibitor concentration confirm the previous studies of Dale (Clin. Chim. Acta, 1977, 77, 407-413, Lancet, 1980, ii, 975) in opposition to those of Brock (Lancet, 1981, i, 95). Non NTD amniotic fluids from 92 women between the 17th and 20nd weeks of pregnancy were used to establish normal values. The technique proposed is rapid, accurate and needs only 40 microliters sample.
D000110:Acetylcholinesterase / Q000032:analysis*; D000653:Amniotic Fluid / Q000201:enzymology*; D002498:Centrifugation / Q000295:instrumentation; D004796:Clinical Enzyme Tests / Q000379:methods; D005260:Female; D006801:Humans; D009436:Neural Tube Defects / Q000175:diagnosis; D011247:Pregnancy; D011262:Pregnancy Trimester, Second
D000110:Acetylcholinesterase
Détermination des activités acétylcholinestérasiques du liquide amniotique sur analyseur centrifuge.