title: Use of the enzyme-linked immunosorbent assay for detection of antibodies to Chlamydia trachomatis.
abstract: An enzyme-linked immunosorbent assay (ELISA) using a soluble antigen prepared from the D strain of Chlamydia trachomatis was used for titration of IgG antibodies to Chlamydia trachomatis in 153 sera from 126 patients with non-specific genital infection and from 27 healthy subjects. The results were compared to those obtained with the micro-immunofluorescence method and were in complete agreement in 143 of the 153 sera. The ELISA proved to be reproducible and as sensitive as the micro-immunofluorescence method.
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D002692:Chlamydia trachomatis / Q000276:immunology*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis
chemical_list: D000907:Antibodies, Bacterial; D003470:Culture Media; D007075:Immunoglobulin M