Study of the interaction of Saccharomyces cerevisiae with glucose by particle microelectrophoresis.
Saccharomyces cerevisiae NCYC 239 in the presence of glucose at temperatures under 303 K shows a time-dependent lowering of electrophoreric mobility v. At temperatures above 303 K, this time-dependent change in v is in the direction of increased mobilities. Cells suspended in buffer indicate a surface pKa of less than 4, whereas for cells suspended in buffered glucose it is impossible to derive a surface pKa. A kinetic study of the interaction of S. cerevisiae with glucose as a function of temperature allows calculation of an activation energy of 140 kJ X mol-1 for the combined processes of (i) uptake of glucose onto the cell wall, (ii) transfer through the cell wall and membrane, and (iii) the establishment of a steady glucose flux through the wall and membrane.
D001693:Biological Transport, Active; D002151:Calorimetry; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D002473:Cell Wall / Q000378:metabolism; D004586:Electrophoresis; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D013696:Temperature
D005947:Glucose