title: Nucleotide sequence of the promoter region of the citrate synthase gene (gltA) of Escherichia coli.
abstract: The gltA gene, specifying the citrate synthase (EC 4.1.3.7) of Escherichia coli, has been isolated and the nucleotide sequence of a 752 basepair segment containing the gltA promoter and encoding 96 aminoterminal residues of the protein has been defined using the dideoxy/M13 method. The results confirm the location and transcriptional polarity of the gltA gene and indicate that the gltA transcript may contain a long leader sequence of 302-306 nucleotides upstream from the coding region.
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002950:Citrate (si)-Synthase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009876:Operon; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases