Inhibition of nucleic acid biosynthesis in procaryotic and eucaryotic cells by cyanocycline A.
Cyanocycline A was cytotoxic against Meth A cells in vitro, and also showed marked activity against the same cell line grown as an experimental ascites tumor. This antibiotic inhibited nucleic acid synthesis in Escherichia coli and Meth A cells. The antimicrobial activity of the antibiotic was reversed by addition of exogenous herring sperm DNA. Cytofluorometric analysis of cyanocycline A-treated Meth A cells showed an unusual pattern of the relative content of DNA and RNA per cell. These results suggested that cyanocycline A binds to DNA, and that it inhibits nucleic acid synthesis.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D004247:DNA / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005399:Fishes; D007700:Kinetics; D008297:Male; D009287:Naphthyridines / Q000633:toxicity; D014176:Protein Biosynthesis / Q000187:drug effects; D012331:RNA, Fungal / Q000494:pharmacology; D013094:Spermatozoa; D014158:Transcription, Genetic / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D009287:Naphthyridines; D012331:RNA, Fungal; C035070:cyanonaphthyridinomycin; D004247:DNA