Kinetics and mechanism of enzymatic hydrolysis of pivampicillin monolayers.
A study of the enzymatic hydrolysis of pivampicillin (an insoluble penicillin) extended as a monolayer on the aqueous interface at a constant surface pressure has been performed. Penicillinase promotes intensive hydrolysis of the pivampicillin monolayers, inducing their solubility. However, no action was observed with dog liver esterase. The hydrolytic process, which was dependent on the film surface pressure and on the quantity of the injected enzyme, is of the Michaelis-Menten type in two dimensions.
D000667:Ampicillin / Q000031:analogs & derivatives*; D000818:Animals; D004285:Dogs; D004950:Esterases / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008567:Membranes, Artificial; D010405:Penicillinase / Q000378:metabolism; D010917:Pivampicillin / Q000378:metabolism*
D008567:Membranes, Artificial; D010917:Pivampicillin; D000667:Ampicillin; D004950:Esterases; D010405:Penicillinase