Conditional change of DNA replication control in an RNA polymerase mutant of Escherichia coli.
A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and synthesized DNA at 30 degrees C as did the wild type. After a shift to 39 degrees C, a temperature still permissive for growth, the mutant increased its origin concentration more than twofold. After a shift from 39 to 30 degrees C, initiation of DNA replication was inhibited until the normal origin concentration was reestablished.
D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005816:Genetic Complementation Test; D009154:Mutation; D010641:Phenotype; D013696:Temperature; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases