Evidence that chicken hypothalamic luteinizing hormone-releasing hormone is [Gln8]-LH-RH.
As previously reported, chicken LH-RH has been purified in a yield of ca. 2.5 micrograms from 2,000 chicken hypothalmi, and we have estimated its chemical structure to be [Gln8]-LH-RH (1). Based on our previous observation (1), [Gln8]-LH-RH and [Glu8]-LH-RH have been synthesized by the solution method for the purpose of the structural confirmation. Synthetic [Gln8]-LH-RH was confirmed to be identical to natural chicken LH-RH on a HPLC system. For further structural confirmation, chymotryptic and thermolytic peptides from synthetic [Gln8]-LH-RH and natural chicken LH-RH were also identified. Thus, these studies support the view that chicken LH-RH has the following structure; pGlu-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly-NH2, [Gln8]-LH-RH. The gonadotropin releasing potency of synthetic [Gln8]-LH-RH was also in a good agreement with that of natural preparation.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005973:Glutamine / Q000032:analysis*; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis* / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000378:metabolism; D007031:Hypothalamus / Q000032:analysis*; D010455:Peptides / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D051381:Rats
D000596:Amino Acids; D006065:Gonadotropins, Pituitary; D010455:Peptides; D005973:Glutamine; D007987:Gonadotropin-Releasing Hormone