Comparison of carboxymethyl-cellulose cation-exchange chromatography and high-performance liquid chromatography in the purification of guinea-pig insulin from pancreatic extracts.
Guinea-pig insulin was purified from pancreatic extracts either by carboxymethyl-cellulose cation-exchange chromatography with a sodium chloride gradient or by high-performance liquid chromatography (HPLC) on octadecyl silica with mixtures of acetonitrile and phosphate buffer. HPLC proved to be superior to ion-exchange chromatography in the purification of insulin with respect both to time saving and to the purity of the product.
D000818:Animals; D002266:Carboxymethylcellulose Sodium; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006168:Guinea Pigs; D007328:Insulin / Q000302:isolation & purification*; D010184:Pancreatic Extracts / Q000032:analysis*
D007328:Insulin; D010184:Pancreatic Extracts; D002266:Carboxymethylcellulose Sodium