Cross reaction of monoclonal antibodies to human MHC class I and class II products with bovine lymphocyte subpopulations.
Peripheral blood lymphocytes (PBL) from cattle have been separated into T & B cell subpopulations using a panning technique. These T and B cell preparations have then been tested by direct and indirect complement mediated cytotoxicity tests with a series of monoclonal antibodies (Moabs) reacting with HLA class I and class II products. The anti-class I monoclonals were monomorphic or non-reactive and where reactive killed both B and T cells. The anti-class II monoclonals reacted only with B lymphocytes. There was no relationship between their reactivity pattern and any BoLA specificity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D006680:HLA Antigens / Q000145:classification / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens