Enhanced glucose-induced insulin release and endogenous Ca2+ ionophoretic activity in neonatal rat islets following islet-activating protein.
Islet-Activating Protein, purified from the culture medium of Bordetella pertussis, enhanced glucose-induced insulin secretion from cultured neonatal rat islets in a calcium dependent manner. This effect was accompanied by an increase in lipid associated Ca2+ ionophoretic activity, as measured by passage of Ca2+ through multilamellar planar membranes containing islet lipid extracts. These findings suggest that the action of IAP in the neonatal islet may be mediated by enhanced entry of extracellular calcium following an effect on membrane lipid composition.
D000818:Animals; D000831:Animals, Newborn; D001426:Bacterial Proteins / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D037342:Pertussis Toxin; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D010566:Virulence Factors, Bordetella
D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D007328:Insulin; D010743:Phospholipids; D010566:Virulence Factors, Bordetella; C025673:calciphorin; D037342:Pertussis Toxin; D005947:Glucose