title: Control of pulsatile LH release in male rats.
abstract: Distinct, short-term pulses of pituitary luteinizing hormone (LH) release are a characteristic feature of tonic LH secretion in normal rats. LH pulses are more pronounced, more frequent, and more regular in castrated rats. Using castrated rats, we sought to identify the basis of the pulsatile discharge of LH by the pituitary gland. Indwelling atrial cannulae were used to obtain frequent blood samples through 3-4 h from three cohorts of conscious, freely moving, orchidectomized male rats. Rats were (1) untreated castrates, (2) infused with ovine antiserum to LHRH or control serum, or (3) infused with a luteinizing hormone releasing hormone (LH-RH) analog, [D-pGlu1,D-Phe2, D-Trp3,6]-LH-RH. Castrates exhibited a pulsatile pattern of circulating LH levels; the mean (+/- SE) peak level of LH pulses was 640 +/- 15 ng/ml, with a mean (+/- SE) pulse period of 18.5 +/- 0.8 min. LH-RH antiserum arrested pulsatile LH secretion immediately, leaving plasma LH levels at 80-120 ng/ml. The LH-RH analog caused a similar suppression of LH release, although this effect was of a shorter duration than the suppression of LH pulses induced by LH-RH antiserum. The obliteration of LH pulses by anti-LH-RH and suppression of LH release by an LH-RH antagonist indicate that the pulsatile secretion of LH is due to corresponding stimulation of the pituitary gland by hypothalamic LH-RH. Anti-LH-RH and an LH-RH antagonist are identified as valuable probes for the experimental dissection of blood-borne signals within the rat hypothalamic-pituitary-testicular axis.
mesh_terms: D000818:Animals; D002369:Castration; D004305:Dose-Response Relationship, Drug; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000276:immunology / Q000502:physiology*; D007106:Immune Sera / Q000008:administration & dosage; D007700:Kinetics; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007106:Immune Sera; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone