Crossbridge release and alpha-helix-coil transition in myosin and rod minifilaments.
The relationship between crossbridge release and alpha-helix-coil transition in myosin has been investigated by employing synthetic myosin and rod minifilaments prepared in 10 mM-citrate/Tris buffer at pH 7.0 and 8.0. Initial sedimentation velocity and turbidity measurements have established that the minifilament structures obtained at pH 7.0 and 8.0 are relatively similar in size and homogeneity, and can be used in comparative circular dichroism studies. Chemical crosslinkings and proteolytic digestions carried out at pH 7.0 and 8.0 verify that myosin and rod minifilaments undergo the same pH-induced changes as myosin filaments, i.e. a decrease in the rate of subfragment-2 crosslinking to the filament surface, and an increase in proteolytic susceptibility of the light meromyosin-heavy meromyosin hinge at alkaline pH. These results suggest charge-induced release of the S-2 element from the myosin and rod minifilament surface. Circular dichroism measurements reveal a reduced alpha-helical content of myosin (5%) and rod minifilaments (10%) at pH 8.0 compared to the respective pH 7.0 structures. These results establish a direct link between crossbridge release and alpha-helix-coil transition in myosin.
D000818:Animals; D002918:Chymotrypsin; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D003599:Cytoskeleton; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D015879:Myosin Subfragments; D009218:Myosins; D010446:Peptide Fragments; D011487:Protein Conformation; D011817:Rabbits; D014461:Ultracentrifugation
D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D015879:Myosin Subfragments; D010446:Peptide Fragments; D002918:Chymotrypsin; D009218:Myosins