title: RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance.
abstract: Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the beta-subunit of RNA polymerase. Some functional properties of the corresponding mutant strains and their RNA polymerases have been investigated.
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005799:Genes, Dominant; D009154:Mutation; D010957:Plasmids; D012318:RNA Polymerase I / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D012293:Rifampin