Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus.
Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002462:Cell Membrane / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D005796:Genes; D005814:Genes, Viral; D007425:Intracellular Membranes / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013347:Subcellular Fractions / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D012333:RNA, Messenger; D014764:Viral Proteins