Inductive properties of fibroblastic cell cultures derived from rat intestinal mucosa on epithelial differentiation.
The present study represents a first attempt to elucidate the regulatory properties displayed by the non-epithelial portion of the intestinal mucosa, growing as fibroblasts in monolayer cultures. Thus, we compared the inductive action of 6-day suckling rat duodenal fibroblasts with that displayed by chick embryonic intestinal mesenchyme on the heterotypic cytodifferentiation of 5 1/2-day chick embryonic gizzard endoderm. The latter, isolated by 0.03% collagenase, was surrounded by intestinal intramucosal fibroblastic cell sheets. As control experiments, fibroblastic cells derived from the intestinal muscle or from 20-day fetal rat skin and lung were used. Every type of association was grafted into the coelomic cavity of 3-day chick embryos for 11 to 12 days, a system providing their vascularization and growth. The results clearly demonstrate that the mucosal fibroblastic cells of rat intestine were as potent as embryonic intestinal mesenchyme in inducing brush-border enzymes like sucrase and maltase, in conformity with an induced intestinal morphology. In contrast, the control fibroblastic cells were completely ineffective.
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004627:Embryonic Induction; D004707:Endoderm / Q000166:cytology; D004847:Epithelial Cells; D005347:Fibroblasts / Q000502:physiology*; D005895:Gizzard, Avian / Q000196:embryology* / Q000201:enzymology; D007365:Intercellular Junctions / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000166:cytology*; D008871:Microvilli / Q000201:enzymology / Q000648:ultrastructure; D009024:Morphogenesis; D051381:Rats; D011919:Rats, Inbred Strains; D013393:Sucrase / Q000096:biosynthesis; D000520:alpha-Glucosidases / Q000096:biosynthesis
D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D013393:Sucrase