Biochemical studies on the potentiation of antitumor activity of cisplatin by adriamycin.
The RNA synthesis in vitro by E. coli RNA polymerase was found to be highly sensitive to cis-diamminedichloroplatinum (cisplatin) inhibition. The degree of inhibition was in proportion to the length of time of template preincubation with cisplatin, suggesting that cisplatin-template binding was involved in the inhibition of RNA polymerase. The effect of adriamycin on this inhibition was studied and it was found that adriamycin significantly enhanced the inhibitory effect of cisplatin and that the total effect was greater than the sum of the effects of each drug used individually. This synergistic effect was not observed when the effect of the combination of adriamycin and cisplatin on in vitro DNA synthesis was studied.
D002945:Cisplatin / Q000494:pharmacology*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004357:Drug Synergism; D004926:Escherichia coli / Q000201:enzymology; D011067:Poly dA-dT / Q000378:metabolism; D013698:Templates, Genetic; D013997:Time Factors
D011067:Poly dA-dT; D004317:Doxorubicin; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase; D002945:Cisplatin