[Angiotensin II potentiates the effect of ACTH on the production of cyclic AMP by bovine adrenal cells in primary culture].
The effect of Angiotensin II (AII) on ACTH-induced cyclic AMP production was studied in bovine adrenocortical cells cultured in a chemically defined serum-free medium. Immediately after collagenase dispersion, AII did not modify either the basal or the ACTH-induced cAMP production by the isolated cells. During cell culture, AII alone did not affect cAMP production. But 2 days after plating, AII increased significantly the ACTH-induced cAMP production by the culture. This potentiating effect increased with the age of culture. Sar1Ala8AII (Saralasin), a potent AII antagonist, inhibited the AII potentiating effect indicating an AII specific action.
D000302:Adrenal Cortex / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003345:Corticosterone / Q000096:biosynthesis; D003366:Cosyntropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004357:Drug Synergism; D012504:Saralasin / Q000494:pharmacology; D013997:Time Factors
D000804:Angiotensin II; D003366:Cosyntropin; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D012504:Saralasin; D003345:Corticosterone
L'angiotensine II potentialise l'effet de l'ACTH sur la production d'AMP cyclique par les cellules surrénaliennes bovines en culture primaire.