title: Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase.
abstract: The sequence of a 5-kilobase DNA insert containing the structural gene for yeast cytoplasmic methionyl-tRNA synthetase has been determined and a unique open reading frame of 2,253 nucleotides encoding a polypeptide chain of 751 amino acids (Mr, 85,500) has been characterized. The data obtained on the purified enzyme (subunit size, amino acid composition, and COOH-terminal sequence) are consistent with the gene structure. The protein sequence deduced from the nucleotide sequence reveals no obvious internal repeats. This protein sequence shows a high degree of homology with that of Escherichia coli methionyl-tRNA synthetase within a region that forms the putative methionyl adenylate binding site. This strongly suggests that both proteins derive from a common ancestor.
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology; D005796:Genes; D008718:Methionine-tRNA Ligase / Q000235:genetics*; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D004247:DNA; D000604:Amino Acyl-tRNA Synthetases; D008718:Methionine-tRNA Ligase