Competence and progression growth factors stimulate different tRNAlys modification reactions in BALB/C 3T3 cells.
Sparse cultures of Balb/C 3T3 cells were growth arrested in a medium containing 1% calf plasma. This treatment caused a decrease in tRNA4lys, a tRNA species related to cell division, and a corresponding increase in tRNA5lys. A restoration of tRNA4lys levels and an increase in cell number was obtained by the addition of either 10% calf serum or a combination of fibroblast growth factor and melanocyte stimulating activity. Neither growth factor alone evoked a proliferative response. The progression factors, insulin and multiplication stimulating activity stimulated the rapid conversion of tRNA5lys to tRNA2lys, whereas the competence factors, fibroblast growth factor and platelet-derived growth factor stimulated a slight conversion of tRNA2lys to tRNA4lys.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*
D006133:Growth Substances; D007328:Insulin; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D012346:RNA, Transfer, Amino Acyl; D005346:Fibroblast Growth Factors; D007335:Insulin-Like Growth Factor II