Studies on a new lymphocyte mitogen from pollen aqueous extract: induction of proliferation in human and rodent lymphocytes.
Fraction III (Fr III) mitogen was isolated from the whole aqueous extract (WAE) of plane-tree pollen by Con A-Sepharose chromatography and gel filtration. It stimulated mouse spleen cells, and peripheral blood lymphocytes from plane-tree non-sensitive patients or from cord blood. A 6-day culture gave the best response. The proliferation was not abolished by washing the cells after a short pulse of Fr III given in the initial step but was inhibited by alpha-D-methylmannoside. These results suggested that Fr III bound to a specific site onto spleen cells. This mitogen was not immunogenic in mice and did not bind to human IgE. It induced the proliferation of T lymphocytes in presence of accessory cells and was a polyclonal activator.
D000293:Adolescent; D000328:Adult; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007108:Immune Tolerance; D007163:Immunosorbent Techniques; D007231:Infant, Newborn; D007376:Interleukin-2 / Q000302:isolation & purification / Q000502:physiology*; D007700:Kinetics; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010936:Plant Extracts / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
D007376:Interleukin-2; D010936:Plant Extracts