Use of Lucifer yellow VS as a label in fluorescent immunoassays illustrated by the determination of albumin in serum.
The use of a new label for fluoroimmunoassay is described. Lucifer yellow VS is a highly fluorescent vinyl sulphone dye which, under mild conditions, forms covalent bonds with amino and sulphydryl groups but is extremely stable in water. A large Stokes shift (110 nm) and an emission maximum at 540 nm give Lucifer yellow further advantages over the more commonly used labels. The use of the dye as a label has been demonstrated by developing a heterogeneous fluoroimmunoassay for human serum albumin. The fluoroimmunoassay gave comparable results to those obtained using a less specific colorimetric dye-binding assay (r = 0.97, n = 20). The advantages, limitations, and other potential uses of Lucifer yellow are discussed.
D003124:Colorimetry / Q000379:methods; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007546:Isoquinolines; D012709:Serum Albumin / Q000032:analysis*
D005456:Fluorescent Dyes; D007546:Isoquinolines; D012709:Serum Albumin; C017475:lucifer yellow