Local biotransformation of des-Tyr1-gamma-endorphin in brain studied by a push-pull technique and HPLC analysis.
The biotransformation of [3H]des-Tyr1-gamma-endorphin locally in rat brain was studied by perfusion of the peptide via a push-pull cannula implanted in the nucleus caudatus. Metabolites were analyzed by high-pressure liquid chromatography. Free [3H]phenylalanine and a [3H]peptide with chromatographic properties of beta-endorphin2-13 were formed during push-pull perfusion. The method presented enables the detection of local proteolytic events in the brain and provides a novel tool for studies on neuropeptide biotransformation.
D000818:Animals; D001711:Biotransformation; D001921:Brain / Q000378:metabolism; D002421:Caudate Nucleus / Q000201:enzymology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004723:Endorphins / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010477:Perfusion / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D004723:Endorphins; D010446:Peptide Fragments; C018903:gamma-endorphin, des-Tyr(1)-; D010447:Peptide Hydrolases