Enzyme immunoassay for methamphetamine.
A competitive enzyme immunoassay for methamphetamine with alkaline phosphatase labeled methamphetamine, Sepharose-antibody and p-nitrophenylphosphate as substrate was developed. The anti-methamphetamine antisera produced in rabbits by immunization with N-(4-aminobutyl) methamphetamine-BSA conjugate were specific for methamphetamine and showed low cross-reactivities with p-OH methamphetamine and amphetamine (metabolites of methamphetamine). The range of methamphetamine measurable by the enzyme immunoassay was 1 to 300 ng/tube. According to the assay, methamphetamine could be detected from urine and extract of hair.
D000469:Alkaline Phosphatase; D000917:Antibody Formation; D005455:Fluorescent Antibody Technique; D006197:Hair / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification; D008694:Methamphetamine / Q000032:analysis*; D019966:Substance-Related Disorders / Q000652:urine
D007074:Immunoglobulin G; D008694:Methamphetamine; D000469:Alkaline Phosphatase