Dipeptidyl carboxypeptidase-deficient mutants of Salmonella typhimurium.
Mutants of Salmonella typhimurium deficient in dipeptidyl carboxypeptidase have been isolated by screening for clones unable to use N-acetyl-L-alanyl-L-alanyl-L-alanine (AcAla3) as the sole nitrogen source. An insertion of the transposable element Tn10 near dcp (the locus coding for dipeptidyl carboxypeptidase) has been isolated and used to map the locus in the interval between purB and trp, an otherwise genetically silent region of the S. typhimurium map. All dcp mutants could still grow using N-acetyl-L-alanyl-L-alanyl-L-alanyl-L-alanine (AcAla4) as the sole nitrogen source. Crude extracts from the dcp mutants failed to hydrolyze AcAla3 but retained approximately 80% of the wild-type activity toward AcAla4. Several lines of evidence indicate that hydrolysis of AcAla4 in the dcp mutant results from the action of a new peptidase distinct from dipeptidyl carboxypeptidase. A mutant strain lacking dipeptidyl carboxypeptidase in addition to peptidases N, A, B, and D showed reduced protein breakdown during carbon starvation compared with a strain lacking only peptidases N, A, B, and D.
D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D002874:Chromosome Mapping; D010450:Endopeptidases / Q000235:genetics*; D009154:Mutation; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*
C037418:acetylalanyl-alanyl-alanyl-alanine; C024933:acetyltrialanine; D002216:Captopril; D010450:Endopeptidases; C020071:dipeptidyl carboxypeptidase; D000409:Alanine