Loss of expression of transplantation antigens encoded by the H-2K locus on Lewis lung carcinoma cells and its relevance to the tumor's metastatic properties.
The Lewis lung carcinoma 3LL, a C57BL/6 (H-2b)-originated spontaneous tumor that progresses at the site of transplantation in all mouse strains, produced lung metastases only in mice that shared the H-2D region and the non-H-2 genetic background with the tumor's strain of origin. In vitro cytotoxicity assays revealed that 3LL tumor cells were sensitive to lysis exerted by anti-H-2Db immune effector cells but were relatively resistant to lysis by anti-H-2Kb effector cells. In addition, the 3LL tumor cells could inhibit the anti-H-2Dd response against EL 4 (H-2b) target cells but had almost no inhibitory effect on the anti-H-2Kb response against the same targets. Immunization of C3HeB (H-2k) and B10.D2 (H-2d) mice with 3LL tumor cells resulted in antisera that could preferentially react with H-2Db-positive cells but reacted very weakly with H-2Kb-positive cells. The results indicate that 3LL tumor cells expressed H-2Db cell surface antigens at a high density and seemed to lack membrane glycoproteins that are encoded by the H-2Kb region.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000032:analysis*; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000648:ultrastructure; D008285:Major Histocompatibility Complex; D051379:Mice; D051381:Rats; D013154:Spleen / Q000276:immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006183:H-2 Antigens