Cytotoxic activity of lymphocytes from F 344 rats bearing intraocular tumor derived from human adenovirus 12-induced retinoblastoma-like cell line.
Lymphocyte cytotoxicity using 51Cr releasing assay was investigated in 10 F344 rats bearing intraocular tumor derived from human adenovirus 12 (Ad 12)-induced retinoblastoma -like cell line (EXP-5). Lymphocytes obtained from tumor bored animal (1 X 10(6)/well) incubated with 51Cr-labelled EXP-5 cells (1 X 10(5)/well) for 24 hours, and counted by beta-scintillation counter. The cytotoxic activity of lymphocytes of transplanted animals was higher in 3 out of 10 subjected rats (24-30%) than in 10 rats of control (3-5%). The results support the view that the correspond animal model in its resemblance and suggested that the rats with retinal tumor have concomitant cell-mediated immunity in the early stage of tumor bearing.
D000257:Adenoviridae Infections / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005134:Eye Neoplasms / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology*; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D012175:Retinoblastoma / Q000276:immunology*