[Stoichiometry of GTP breakdown during peptide synthesis on the ribosome. Stoichiometry of GTP hydrolysis during elongation of polyphenylalanine on polyuridylic acid].
The stoichiometry of GTP hydrolysis during poly(Phe) elongation by Phe on poly(U) covalently bound to Sepharose was determined. The concentrations of both EF-T and EF-G were saturating. The GTP/Phe stoichiometry was calculated without the usual correction for the uncoupled ribosomal EF-T and EF-G dependent GTP hydrolysis. At the Mg2+ optimum (6 mM) for the poly(Phe) elongation on poly(U) . Sepharose the stoichiometry ratio of GTP/Phe was 1.9/2.1. This indicates that two (or less) GTP molecules coupled with poly(Phe) elongation by Phe on poly(U) . Sepharose are hydrolyzed.
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D010441:Peptide Chain Elongation, Translational; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010455:Peptides / Q000235:genetics*; D011072:Poly U / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism*
D010445:Peptide Elongation Factors; D010455:Peptides; D011072:Poly U; C012001:polyphenylalanine; D006160:Guanosine Triphosphate; D008274:Magnesium
Izuchenie stekhiometrii raspada GTP pri sinteze peptida na ribosome. Stekhiometriia gidroliza GTP v protsesse élongatsii polifenilalanina na poliuridilovoĭ kislote.