The existence of inactive (trypsin-activated) renin in dog plasma and renin granules from the kidney.
Trypsin-activated renin (inactive renin) was detected in the break-through fraction when dog plasma or renin extracted from renin granules (stored renin) was applied to a pepstatin column, respectively. The appearance of the renin activity by trypsin treatment was not due to acid protease. Production of angiotensin I from homologous renin substrate by the trypsin-activated renin was proportional to the time of incubation. The trypsin-activated renin had an affinity for the pepstatin column. The maximum amount of trypsin-activated renin was obtained with incubation for 15 min at 37 degrees C at 1000 micrograms/ml in plasma or at 100 micrograms/ml in case of stored renin. The ratio of inactive to active renin was calculated to be 1.6 or 0.002 in plasma or stored renin, respectively, under conditions of a standard sodium diet.
D000818:Animals; D016282:Aspartic Acid Endopeptidases; D002846:Chromatography, Affinity; D003594:Cytoplasmic Granules / Q000201:enzymology; D004285:Dogs; D010450:Endopeptidases / Q000378:metabolism; D004792:Enzyme Precursors / Q000097:blood / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D012083:Renin / Q000097:blood / Q000378:metabolism*; D013696:Temperature; D014357:Trypsin / Q000378:metabolism
D004792:Enzyme Precursors; D010450:Endopeptidases; D014357:Trypsin; D016282:Aspartic Acid Endopeptidases; D012083:Renin