A one-step procedure for the purification of uridine phosphorylase from Escherichia coli.
Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000302:isolation & purification*; D014543:Uridine Phosphorylase / Q000302:isolation & purification*
D010430:Pentosyltransferases; D014543:Uridine Phosphorylase