title: Cells on microspheres: a new technique for flow cytometric analysis of adherent cells.
abstract: Technical problems have previously prevented the application of fluorescence activated cell sorting to the study of adherent cell populations. We have developed a procedure for attachment of human monocyte-macrophages to 14-20 micrometer microspheres. These adherent cells on microspheres retained phagocytic capacity, could be stained for cell surface antigens using indirect immunofluorescence, and could be maintained in long-term culture. They could be examined and sorted on a fluorescence activated cell sorter while still in the adherent state. This technique facilitates the flow cytometric study of adherent cells and permits the isolation of subpopulations of these cells.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008863:Microspheres; D009000:Monocytes / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis; D011817:Rabbits
chemical_list: D000949:Histocompatibility Antigens Class II