N-(7-dimethylamino-4-methylcoumarinyl)-maleimide (DACM): an alternative label for fluorescence tracing.
N-(7-dimethylamino-4-methylcoumarinyl)maleimide (DACM), a fluorescent thiol reagent, has a high molar extinction coefficient and a high quantum yield when it reacts with protein sulphhydryl bonds. DACM proved to be effective as a fluorescent probe when used for labelling rabbit immunoglobulin G anti-human gamma-chain (IgG). DACM-labelled rabbit immunoglobulin G anti-human gamma-chain conjugates (DACM-IgG) can be purified efficiently by Sephadex G-50 gel chromatography. The immunological properties of the IgG are not altered appreciably by conjugation. As a label DACM has advantages with regard to ease of preparation, specific immunofluorescence and strong photoresistance.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007678:Kidney Glomerulus / Q000276:immunology; D008301:Maleimides / Q000378:metabolism*; D014066:Palatine Tonsil / Q000276:immunology; D011817:Rabbits; D051381:Rats; D013270:Stomach / Q000276:immunology
D007074:Immunoglobulin G; D008301:Maleimides; C019347:N-(7-dimethylamino-4-methylcoumarinyl)maleimide