title: Basolateral maturation of retroviruses in polarized epithelial cells.
abstract: We have investigated the maturation sites of avian and mammalian C-type retroviruses in polarized epithelial cells. Examination of thin sections of Madin Darby canine kidney cells infected with RD114 or avian reticuloendotheliosis virus revealed that these viruses mature from the basolateral membrane domains. Similar results were obtained with a continuous line of mouse mammary epithelial cells infected with Friend, Moloney, Rauscher, or Kirsten murine leukemia viruses, or Friend virus-related or Moloney virus-related mink cell focus-forming viruses. Immunofluorescence observations indicate that viral glycoproteins are inserted only at the basolateral membranes in these cells. Because of the availability of DNA and protein sequence data, and of molecularly cloned viruses, these virus systems offer advantages for molecular studies on directional transport of plasma membrane glycoproteins.
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D004285:Dogs; D004848:Epithelium / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag; D007668:Kidney; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D012190:Retroviridae / Q000254:growth & development* / Q000648:ultrastructure; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism; D014776:Virus Cultivation
chemical_list: D015683:Gene Products, gag; D014759:Viral Envelope Proteins; D014764:Viral Proteins