Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.
The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strain. These have been used to produce full-length DNA copies of the two genomes each within a single plasmid.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics*; D013045:Species Specificity
D004247:DNA; D004262:DNA Restriction Enzymes