Enzyme-linked immunosorbent assay for quantitation of islet cell antibodies.
We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of islet antibodies (ICA) in sera from insulin-dependent diabetic (IDD) subjects or from Bio-Breeding/Worcester (BB/W) rats. Whole rat or mouse islet cells, either glutaraldehyde-fixed or desiccated, which can be stored and used over a long period, were used as antigens. The amount of antibody bound to the cells is quantitated by the addition of sheep anti-human or anti-rat IgG conjugated with beta-galactosidase. This quantitative ELISA compared favourably with other assays for ICA detection.
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007330:Insulin Antibodies / Q000032:analysis*; D007515:Islets of Langerhans / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D051381:Rats; D011917:Rats, Inbred Lew
D007330:Insulin Antibodies