The occurrence of N-acetyl- and N-glycoloylneuraminic acid in Trypanosoma cruzi.
Different strain of Trypanosoma cruzi were analysed to have 65--105 micrograms of sialic acids per 10(10) cells. By thin-layer chromatography, and in part by gas liquid chromatography and gas-liquid chromatography-mass spectrometry, all strains were found to contain N-acetyl- and N-glycoloylneuraminic acid in various ratios. After incubation of the parasites with either [3H]acetate or N-acetyl-[3H]mannosamine, no radioactivity was found in the sialic acids, thus leading to the suggestion that the parasites are unable to synthesize sialic acids from their precursors.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D019158:N-Acetylneuraminic Acid; D009438:Neuraminic Acids / Q000096:biosynthesis / Q000302:isolation & purification*; D012794:Sialic Acids / Q000096:biosynthesis / Q000302:isolation & purification*; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000032:analysis*
D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid; D019158:N-Acetylneuraminic Acid