The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis.
The rfaD gene product, ADP-L-glycero-D-mannoheptose-6-epimerase, is necessary for the conversion of ADP-D-glycero-D-mannoheptose to ADP-L-glycero-D-mannoheptose. The nucleotide ADP-D-glycero-D-mannoheptose accumulates in rfaD mutant strains. Two chimeric colicin E1 plasmids carrying the coding sequence of the rfaD+ locus have been selected and shown to complement the rfaD phenotype. These clones also result in an amplification of ADP-L-glycero-D-mannoheptose-6-epimerase activity.
D002238:Carbohydrate Epimerases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005838:Genotype; D007700:Kinetics; D008070:Lipopolysaccharides / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D013045:Species Specificity; D013379:Substrate Specificity
D008070:Lipopolysaccharides; C037213:ADP-L-glycero-D-mannoheptose-6-epimerase; D002238:Carbohydrate Epimerases