Macrophage migration inhibition factor against cell-surface antigens coded by the major histocompatibility complex and other genes in mice.
We developed an indirect capillary tube method to improve reproducibility of macrophage migration inhibition (MI) tests using a one-way mixed lymphocyte culture. MI response could be induced to cell-surface antigens coded by either H-2 or non-H-2 (background) genes. The sensitivity was more readily induced across H-2 + background differences. The presence of only background difference did not induce the MI response to much extent. High MI activities were obtained to antigens coded by either K end or D end of the H-2 complex + background difference. Moderate activities were induced across the H-2D difference + background. These results suggest that the D region of the H-2 complex may direct a MI response when an H-2I difference is present during sensitization.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D006183:H-2 Antigens / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis*; D008264:Macrophages / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains
D000954:Antigens, Surface; D006183:H-2 Antigens; D008263:Macrophage Migration-Inhibitory Factors