title: The B-L (Ia-like) antigens of the chicken. Lymphocyte plasma membrane distribution and tissue localization.
abstract: Specific antisera reacting with B-L (Ia-like) antigens were prepared by reciprocal immunization of animals from the congeneic lines CB and CC. The resulting antisera were tested either in direct or indirect immunofluorescence tests and stained 10-16% of peripheral blood cells (PBL). Of the B-L+ cells, 90% were B cells and 8% were T cells. After in vitro stimulation of PBL with ConA, 58% were B-L+ and 91% of these were T cells. B and T cells were defined by means of rabbit antisera raised against bursa and thymus cells made specific by absorption with the relevant tissues. Antigens determined by anti-B-L antisera, rabbit anti-bursa (ABS) and rabbit anti-thymus (ATS) sera showed an independent distribution on the membrane of PBL. The tissue distribution of B-L+ cells, defined by means of allo-antisera and monoclonal antibodies, was studied by direct and indirect immunofluorescence on sections of skin, liver, kidney and brain. In all organs, in addition to B cells and a small number of, presumably activated, T cells, macrophages and dendritic cells were positive. Notably, glia cells in the brain were also shown to express B-L antigen.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001921:Brain / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification*; D008214:Lymphocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000949:Histocompatibility Antigens Class II