Hydrophobic interaction between DNP-HSA and different tissue structures in vivo assessed by indirect immunofluorescence microscopy.
Circulating dinitrophenylated human serum albumin (DNP-HSA) was shown by indirect immunofluorescence microscopy to adhere to non-parenchymal liver cells and capillaries of striated muscle in mice. The DNP-HSA at these sites could be removed by surface-tension-lowering agents such as Triton X-100 and ethylene glycol. The adherence of DNP-HSA to white blood cells in vitro was inhibited in the presence of 0.3 M ethylene glycol. The findings support the hypothesis that hydrophobic interaction with different tissue structures may be important for the fate of circulating antigens and immune complexes. Indirect immunofluorescence microscopy offers a simple means of checking the role of hydrophobic bonding for the tissue adherence of various substances in vivo.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002448:Cell Adhesion; D004140:Dinitrophenols; D005455:Fluorescent Antibody Technique; D007962:Leukocytes / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000276:immunology*; D012709:Serum Albumin
D000954:Antigens, Surface; D004140:Dinitrophenols; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate