An enzyme-linked immunosorbent assay (ELISA) for field diagnosis of visceral leishmaniasis.
A simplified enzyme-linked immunosorbent assay (ELISA) was evaluated as a diagnostic test for visceral leishmaniasis in the field on 222 individuals with splenomegaly and 110 controls. The test was shown to have a sensitivity of 98.4% and specificity of 100% when compared with parasite identification by splenic aspiration. The data indicate that the ELISA is an accurate, safe, and economical alternative to splenic aspiration for the diagnosis of visceral leishmaniasis.
D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007891:Leishmania / Q000254:growth & development; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D013154:Spleen / Q000469:parasitology