[Effect of glycerin removal on the electrical resistance of isolated muscle fiber in the frog].
Fibres isolated from iliofibularis muscles of the frog Rana esculenta were studied under current-clamp conditions with a double sucrose-gap technique. An increase of membrane resistance in muscle fibres (Rm) was demonstrated during the first 10-15 min of glycerol removal in K2SO4 solution. After a 30 min treatment in glycerol-containing K2SO4 Rm was 1.40 +/- 0.12 k omega X X cm2. The transfer of muscles from the glycerol-K2SO4 solution to an isotonic K2SO4 solution resulted in a progressive increase in the Rm which after a 15 minutes removal of glycerol reached the mean value of 2.02 +/- 0.22 k omega X cm2. It is suggested that this increase may reflect detubulation of the fibres caused by glycerol removal in muscles.
D000818:Animals; D004553:Electric Conductivity; D005990:Glycerol / Q000494:pharmacology*; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000187:drug effects* / Q000502:physiology; D011893:Rana esculenta; D013431:Sulfates / Q000494:pharmacology; D013997:Time Factors
D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D013431:Sulfates; C031512:potassium sulfate; D005990:Glycerol
Vliianie otmyvki glitserina na élektricheskoe soprotivlenie izolirovannogo myshechnogo volokna liagushki.