Immunological analysis of v-myc gene products using antibodies against a myc-specific synthetic peptide.
Rabbit antisera were prepared against a synthetic peptide corresponding to the carboxyterminal amino acid sequences of the transforming protein p110gag-myc of avian oncovirus MC29. Analysis of immunoprecipitates formed with these sera (anti-mycC) demonstrated that the gag-myc hybrid proteins encoded by the avian leukemia viruses MC29, CMII, and OK10 were recognized by the anti-peptide sera, but not the gag or pol precursor proteins of helper viruses or the MH2-encoded p100gag-mil protein. In cells transformed by OK10 or MH2, putative v-myc proteins of 60K (OK10) and 59/61K (MH2) were also precipitated by anti-mycC. In addition, the anti-peptide sera reacted specifically with the gag-myc proteins encoded by three partially transformation-defective mutants of MC29, td10A, td10C, and td10H, and by MC29 variant HBI.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian; D005347:Fibroblasts; D015683:Gene Products, gag; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D011784:Quail; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D000906:Antibodies; D015683:Gene Products, gag; D014764:Viral Proteins