Antigen presentation by supported planar membranes containing affinity-purified I-Ad.
I-Ad, purified from A20-1.11 cells by affinity chromatography, was incorporated into supported planar membranes by incubation of I-Ad-containing phospholipid vesicles with clean glass coverslips. Such planar membranes present a peptide digest of ovalbumin to the ovalbumin-specific, I-Ad-restricted T-cell hybridoma 3DO-54.8, resulting in the antigen-specific release of interleukin 2. However, when the same material was provided in the form of small unilamellar vesicles, no response was obtained. Antigen presentation by the I-Ad-containing planar membranes was inhibited by the monoclonal antibody MKD6 (anti-I-Ad) but not by the antibody 10-2.16 (anti-I-Ak). The antibody GK1.5, which recognizes the T-cell surface antigen L3T4, was also inhibitory. In contrast to the results with purified I-Ad, crude membrane preparations from A20-1.11 cells were effective in antigen presentation in both planar and vesicular forms.
D000818:Animals; D000936:Antigen-Antibody Complex; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007376:Interleukin-2 / Q000032:analysis; D008567:Membranes, Artificial; D051379:Mice; D008970:Molecular Weight; D011110:Polymorphism, Genetic
D000936:Antigen-Antibody Complex; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D008567:Membranes, Artificial