Effect of copper or insulin in diabetic copper-deficient rats.
The effects of copper and insulin on lipogenesis and glucose tolerance were studied using diabetic, copper-deficient rats. Diabetes was induced by intraperitoneal injection of 50 mg streptozotocin/kg body weight to rats fed a sucrose-copper deficient diet for 7 weeks. Five days later the rats were injected intraperitoneally with [14C]glucose with either saline, insulin, copper, or copper plus insulin. The disappearance of serum [14C]glucose at 30, 60, and 120 min postinjection and the incorporation of [14C]glucose into lipid of epididymal fat 2 hr after administration were determined. The combined effect of copper and insulin significantly decreased peak blood glucose at 30 min and increased the incorporation of [14C]glucose into lipid in the epididymal fat pad when compared to either copper or insulin alone. The enhancement of glucose utilization may be due to a formation of a more stable complex which will increase insulin binding and/or decrease its degradation.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D002570:Ceruloplasmin / Q000032:analysis; D003300:Copper / Q000008:administration & dosage* / Q000097:blood / Q000172:deficiency; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004357:Drug Synergism; D007328:Insulin / Q000008:administration & dosage* / Q000097:blood; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D007328:Insulin; D003300:Copper; D002570:Ceruloplasmin