Procedure for mutagenizing spores of Saccharomyces cerevisiae.
A procedure for inducing mutants of a homothallic strain of Saccharomyces cerevisiae is described. The essential parts of the procedure are long incubation in Glusulase, which preferentially kills vegetative cells instead of spores, and treatment in 9% ethyl methanesulfonate, which also preferentially kills vegetative cells instead of spores. Consequently, the viable population is virtually 100% spores.
D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005966:Glucuronidase / Q000633:toxicity; D009097:Multienzyme Complexes / Q000633:toxicity; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology; D013429:Sulfatases / Q000633:toxicity
D009097:Multienzyme Complexes; C023059:glusulase; D005020:Ethyl Methanesulfonate; D013429:Sulfatases; D005966:Glucuronidase