Sugar specificity of the receptor of rat thymocytes in rosette formation with guinea pig erythrocytes.
Most rat thymocytes form rosettes with guinea pig erythrocytes only in the presence of nonheated fetal calf serum. The sugar specificity of the receptors of rat thymocytes were studied using the sugar inhibition test with various monosaccharides and glycoproteins, exoglycosidases treatment on rat thymocytes, and adsorption of fetal calf serum with lectins and sugar gels. The following observations were made; L-fucose and D-mannose completely inhibited rosette formation, whereas addition of D-galactose had no inhibitory effect on rosette formation. The rosette-forming ability of thymocytes was reduced by mannosidase treatment, but fucosidase treatment did not show any inhibitory effect on rosette formation. These results suggest that mannose residue on the surface of thymocytes participated in the rosette formation.
D000818:Animals; D002454:Cell Differentiation; D006023:Glycoproteins / Q000276:immunology; D006026:Glycoside Hydrolases; D006168:Guinea Pigs; D009005:Monosaccharides / Q000494:pharmacology; D051381:Rats; D011971:Receptors, Immunologic; D012397:Rosette Formation; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*
D006023:Glycoproteins; D009005:Monosaccharides; D011971:Receptors, Immunologic; D006026:Glycoside Hydrolases