Effects of intrasplenic injection of hepatocytes, hepatocyte fragments and hepatocyte culture supernatants on D-galactosamine-induced liver failure in rats.
Intraperitoneal injection of 0.5 g/kg D-galactosamine results in 95% lethal acute acute liver failure in male Fisher 344 rats. Intrasplenic injection of viable syngeneic hepatocytes 20-28 h after poisoning improves survival in a dose-dependent fashion, 10(7) cells being the optimal dose with a survival rate of 47.1%. While nonviable cells and hepatocyte fragments are totally ineffective, 42.9% of rats survive after injection of 28-hour liver cell culture supernatant. It is concluded that soluble factors generated by cultured cells in vitro or intrasplenically transplanted cells improve survival either by a direct hepatotrophic effect, by stimulation of the reticulo-endothelial system or by an unspecific humoral mechanism.
D000208:Acute Disease; D000818:Animals; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury; D005688:Galactosamine; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen; D014185:Transplantation, Isogeneic
D005688:Galactosamine