The localisation of intracellular immunoglobulin and alpha-1-antitrypsin by immunoelectron staining of post-osmicated, resin-embedded tissue.
An indirect immunoperoxidase method is described to demonstrate intracellular immunoglobulins and alpha-1-antitrypsin in semithin and ultrathin sections from human tissue. The tissue was primarily fixed in glutaraldehyde, post-osmicated and resin-embedded.
D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D008099:Liver / Q000032:analysis / Q000648:ultrastructure; D008854:Microscopy, Electron; D009992:Osmium / Q000494:pharmacology*; D009993:Osmium Tetroxide / Q000494:pharmacology*; D014066:Palatine Tonsil / Q000032:analysis / Q000648:ultrastructure; D010950:Plasma Cells / Q000032:analysis / Q000648:ultrastructure; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000276:immunology
D007136:Immunoglobulins; D000515:alpha 1-Antitrypsin; D009992:Osmium; D009993:Osmium Tetroxide