Regulation of the immune response in experimental models of autoimmune disorders: I. Immunocompetence and transplantation tolerance in female (NZB X NZW)F1 hybrid mice immunosuppressed with total lymphoid irradiation and in reconstituted bone marrow chimeras.
Female (NZB X NZW)F1 (B/WF1) mice treated with fractionated total lymphoid irradiation (TLI) and TLI conditioned BALB/c leads to B/WF1 bone marrow (BM) chimeras were studied to determine the effects of TLI and reconstitution with normal BM on in vivo and in vitro immune reactivity. TLI treated B/WF1 females showed limited increased retention of semiallogeneic skin grafts (2 1/2 times as long as untreated) and allogeneic skin grafts (twice as long as untreated). In vitro proliferative responses to allogeneic lymphocytes were severely depressed at 5 days after TLI with partial recovery as early as 15 days post TLI. Proliferative responses to phytohemmagglutinin, concanavalin A and lipopolysaccharide were similarly depressed in the immediate post TLI period with gradual subsequent recovery. Nine out of eleven B/WF1 mice transplanted with semiallogeneic, BALB/c BM after TLI conditioning were stable chimeras without evidence of graft vs host disease and were permanently tolerant to BALB/c skin allografts in vivo and BALB/c alloantigens in vitro. BALB/c leads to B/WF1 chimeras showed BALB/c type in vitro proliferative mitogen responses without the age related decline in reactivity characteristic of untreated B/WF1. In contrast, adult B/WF1 mice were resistant to induction of completely allogeneic BM chimerism. The significance of these findings as they relate to the impaired immune status of female B/WF1 mice is discussed.
D000818:Animals; D000917:Antibody Formation; D001327:Autoimmune Diseases / Q000276:immunology*; D016026:Bone Marrow Transplantation; D005260:Female; D006085:Graft Survival / Q000528:radiation effects; D007111:Immunity, Cellular; D007121:Immunocompetence / Q000528:radiation effects; D007165:Immunosuppression Therapy; D007519:Isoantigens / Q000276:immunology; D008221:Lymphoid Tissue / Q000528:radiation effects*; D051379:Mice; D008934:Mitogens / Q000276:immunology; D011828:Radiation Chimera; D016038:Skin Transplantation; D014181:Transplantation Immunology / Q000528:radiation effects
D007519:Isoantigens; D008934:Mitogens