Mannan structure analysis of the fragile Saccharomyces cerevisiae mutant VY1160.
The fragile Saccharomyces cerevisiae mutant VY1160 has a cell-wall mannan with a lower molecular weight and a higher nitrogen content than the parental S288C strain. More mannobiose but less mannotriose and mannotetraose were found in O-glycosidically-bound oligosaccharides in the mutant. Acetolysis analysis of the alkali-stable mannan also showed a reduction of mannotetraose and an increase of the mannobiose fractions. Methylation analysis of total mannan, or side chains recovered after acetolysis, showed that in the mutant the amount of alpha(1-3)-linked mannose units prevailed over alpha(1-2)-linked residues.
D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D008351:Mannans / Q000032:analysis*; D009154:Mutation; D009844:Oligosaccharides / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics; D014312:Trisaccharides / Q000032:analysis
D008351:Mannans; D009844:Oligosaccharides; D014312:Trisaccharides; C014647:mannobiose; C039922:mannotetraose; C023872:mannotriose