Arterial renin--partial characterization and its possible role in the pathogenesis of hypertension.
1) Renin-like enzyme of rat aorta was purified by chromatography with DEAE-cellulose and Sephadex G-200. 2) The molecular weight of renin-like enzyme was 124,000 and 72,000 on Sephadex G-200 gel filtration. The isozymes, however, migrated as a single band with molecular weight of 71,000 on SDS/polyacrylamide gel electrophoresis. These isozymes showed the same optimal pH (6.5) and temperature (37 degrees C). 3) Renin-like enzyme showed high activity in the microsomal fraction of the aorta. 4) In one-clip, two-kidney Goldblatt hypertensive rats, the aortic renin concentration increased significantly, but not parallel with the activity in plasma. 5) Renin, widely distributed in subcellular fractions of the aorta, may play a possible role in the local control of vascular tone. It is likely that renin in vascular wall is of local origin.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002850:Chromatography, Gel; D006978:Hypertension, Renovascular / Q000209:etiology*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood / Q000302:isolation & purification / Q000502:physiology*; D013347:Subcellular Fractions / Q000201:enzymology
D012083:Renin