Expression of Lyb-5 and Mls determinants by Peyer's patch B lymphocytes of X-linked immunodeficient mice.
Treatment of the Peyer's patch (PP) B cells from X-linked immunodeficient (xid) (CBA/N X DBA/2)F1 male mice with anti-Lyb-5 plus complement kills approximately 75% of these cells, although xid spleen B cells are unaffected. Cytotoxic depletion of Lyb-5+ cells renders the xid PP B cell population unable to generate an in vitro direct plaque-forming cell response to the TI-2 antigen TNP-Ficoll. In addition, the B cell-enriched population from the PP of xid (CBA/N X CBA/J)F1 male mice were strong stimulators of proliferation in an M1s-defined MLR, thereby demonstrating that they also express the M1s antigen(s). Two cell surface antigens associated with mature B cells are therefore expressed by xid PP B cells, suggesting that the TNP-Ficoll responsive cells in this population are mature B cell.
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008912:Minor Histocompatibility Loci; D010581:Peyer's Patches / Q000166:cytology; D010641:Phenotype; D014301:Trinitrobenzenes / Q000276:immunology
D000950:Antigens, Ly; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll