Pancreatic islet cell suspensions from newborn rats; different preparation procedures, viability and (pro)insulin biosynthesis.
Islet cell suspensions were prepared from neonatal rat pancreatic islets. While mechanical disintegration results in a higher yield, cells prepared by trypsin treatment appear to better preserved. Trypsin treatment of pancreatic islets during the cell preparation procedure does not influence the stimulation by glucose of (pro)insulin biosynthesis in freshly isolated cells.
D000375:Aging; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005947:Glucose / Q000494:pharmacology; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011384:Proinsulin / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin
D011384:Proinsulin; D014357:Trypsin; D005947:Glucose