A method for isolating Trypanosoma cruzi amastigotes from spleen and liver using two-step discontinuous gradient centrifugation.
D000818:Animals; D002499:Centrifugation, Density Gradient; D008099:Liver / Q000469:parasitology*; D051379:Mice; D008805:Mice, Inbred A; D013154:Spleen / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000302:isolation & purification*