Structure of simian virus 40 DNA in primary and derived mouse tumors.
Primary tumors induced by Simian Virus 40 (SV40) in nude mice, lines derived from them, and subsequent transplanted tumors were analyzed as to the state of the viral DNA. It was found that SV40 DNA is present only as integrated molecules, each tumor having a different integration pattern; all tumors were SV40 T-antigen positive by immunofluorescence. One tumor and its derivatives in vitro and in vivo have maintained a single integration structure consisting of a head-to-tail arrangement of a molecule slightly larger than whole-length viral DNA (approximately equal to 1.1). This tumor expresses a full-sized T-antigen and a Mr 54,000 host-associated protein.
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000382:microbiology*; D013539:Simian virus 40 / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes