A microquantitative method for investigating the interaction between labeled lectins and the surface membranes of human lymphocytes using a semi-automatic harvesting machine.
We describe a simple, rapid and economical method for the study of the interaction of labeled lectins and the surface membranes of human lymphocytes using a semi-automatic harvesting machine (Titertek Multiple Cell Harvester). The procedure requires both small numbers of cells and small amounts of lectin, moreover it reduces the number of experimental steps required.
D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000378:metabolism; D003584:Cytological Techniques; D006801:Humans; D037102:Lectins; D007938:Leukemia / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D037121:Plant Lectins; D014316:Tritium
D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014316:Tritium; D003208:Concanavalin A