title: X-ray diffraction study of the zinc(II) binding sites in yeast phenylalanine transfer RNA. Preferential binding of zinc to guanines in purine-purine sequences.
abstract: An X-ray diffraction study of a zinc(II) complex of tRNAPhe from yeast reveals the present of five zinc-binding sites on the tRNA molecule. Two of the cooperatively bound Mg2+ in the native tRNA structure are replaced by Zn2+. The remaining sites involve direct coordination of zinc to the N7 position of tRNA guanine bases, G15, G43 and HG45. Thus, zinc displays a high specificity for binding to guanine bases in purine-purine sequences.
mesh_terms: D001483:Base Sequence; D006147:Guanine; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl; D012441:Saccharomyces cerevisiae; D014961:X-Ray Diffraction; D015032:Zinc
chemical_list: D012346:RNA, Transfer, Amino Acyl; D006147:Guanine; D015032:Zinc