Detection of an Epstein-Barr virus-associated membrane antigen in Epstein-Barr virus-transformed nonproducer cells by leukocyte migration inhibition and blocking antibody.
Soluble membrane fractions derived from Raji cells trigger lymphocytes of Epstein-Barr virus (EBV)-seropositive, but not EBV-seronegative, individuals to release a lymphokine that inhibits leukocyte migration. The reaction can be blocked by the sera of patients with EBV-DNA-carrying tumors, Burkitt lymphoma, or nasopharyngeal carcinoma. Absorption of these sera with EBV-positive, but not EBV-negative, cells abrogates their blocking activity. These findings suggest that the antigen responsible for the leukocyte migration inhibition reaction is an EBV-encoded or an EBV-induced membrane component. The antigen is not identical with EBV-associated nuclear antigen or any other known antibody-detected EBV antigen.
D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002051:Burkitt Lymphoma; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002464:Cell Migration Inhibition; D002472:Cell Transformation, Viral; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000502:physiology*; D007962:Leukocytes / Q000276:immunology*; D008222:Lymphokines / Q000502:physiology*
D000954:Antigens, Surface; D000956:Antigens, Viral; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines