Specificity of mouse hybridoma antibodies to DNA. II. Phospholipid reactivity and biological false positive serological test for syphilis.
Using mouse hybridoma monoclonal antibodies to DNA from MRL/lpr and NZB X NZW (B/W F1) mice, the reactivity of anti-DNA antibodies to several phospholipids was analysed. The anti-DNA antibody which reacted with the common antigenic determinants on the phosphate-sugar backbone of nucleic acid could bind to the cardiolipin, but failed to bind to other phospholipids, including VDRL antigen. We tentatively conclude that the anti-cardiolipin antibody is identical with the anti-DNA antibody, but differs from the BFP reactor.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002308:Cardiolipins / Q000276:immunology*; D002784:Cholesterol / Q000276:immunology; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D005189:False Positive Reactions; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D010713:Phosphatidylcholines / Q000276:immunology; D010716:Phosphatidylinositols / Q000276:immunology; D013588:Syphilis Serodiagnosis
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D002308:Cardiolipins; D004277:DNA, Single-Stranded; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D004247:DNA; D002784:Cholesterol