title: Immunocytochemical localization of acrosin in boar spermatozoa.
abstract: Acrosin was localized on the acrosomal membranes of boar sperm by immunofluorescence and immunoelectron microscopy. Indirect labeling of sperm head sections was performed with an Imposil (iron-dextran) antibody conjugate. The results of electron microscopy indicated a preferential deposition of the antiacrosin antibodies on the outer acrosomal membrane, although a lesser deposition was also found on the inner acrosomal membrane. No labeling was detected in the acrosomal content when such material was still present.
mesh_terms: D000176:Acrosin / Q000276:immunology / Q000378:metabolism*; D000177:Acrosome / Q000201:enzymology; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D010450:Endopeptidases / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D017830:Octoxynol; D011092:Polyethylene Glycols; D013094:Spermatozoa / Q000201:enzymology*; D013552:Swine / Q000378:metabolism*
chemical_list: D011092:Polyethylene Glycols; D017830:Octoxynol; D010450:Endopeptidases; D000176:Acrosin