Activation and proliferation signals in mouse B cells. IV. Concanavalin A stimulates B cells to leave G0, but not to proliferate.
Concanavalin A (Con A), in solution, induces mouse T cells, but not B cells, to proliferate. However, lectin concentrations which were optimal for the T cell response induced purified B cells to depolarize, to enlarge, and to display increased levels of Ia antigens. Furthermore, culturing B lymphocytes with Con A for 24 hr caused the cells to synthesize DNA more rapidly in response to subsequent stimulation by lipopolysaccharide, or anti-immunoglobulin antibodies. This priming effect did not appear to require either T cells or accessory cells. It was therefore concluded that Con A activates resting B lymphocytes, i.e. stimulates them to enter the cell cycle, even though it does not induce B cells to divide.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007399:Interphase; D008213:Lymphocyte Activation; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D008934:Mitogens; D003208:Concanavalin A; D004247:DNA