Developmental aspects of androgen-dependent mRNA from rat ventral prostate using cloned cDNA.
The androgen-dependence of two mRNAs from rat ventral prostate coding for a 20 000 and an 11 000 dalton translation product has been investigated using complementary DNA cloned in the bacterial plasmid PBR322. One of the cloned insert DNAs from a recombinant plasmid, C-27, arrests the in vitro translation of C2 (Peeters et al. (1980) J. Biol. Chem. 255, 7017-7023). The other cloned insert DNA arrests the translation of a glycoprotein 20 000 daltons in size, with unknown function. The quantities of mRNA coding for the 20 000 and 11 000 dalton translation product were determined by hybridization of 32P-labeled inserts to filter-bound total RNA or poly(A+)-mRNA. Castration caused a decline in both mRNAs of 250-fold over 8 days. Stimulation with androgen of 5-week castrates restored the mRNA levels to 17% of intact for the 20 000 dalton translation product and 31% of intact for the 11 000 dalton translation product. The quantity of the two mRNAs found in the lateral poly(A+)-mRNA was about 1/10 that of the ventral level and the mRNAs were not detectable in the dorsal prostate, seminal vesicle or human prostate poly(A+)-mRNA populations. RNA from the ventral prostates of animals 10-21 days old contained mature levels of complementary sequences, suggesting a form of developmental posttranscriptional regulation for synthesis of the polypeptides which are not synthesized in mature quantities at this stage of development (Heyns et al. (1978) Endocrinology 103, 1090-1095; Kistler et al. (1981) Proc. Natl. Acad. Sci. (U.S.A.) 78, 737-741).
D000727:Androgen-Binding Protein / Q000096:biosynthesis; D000818:Animals; D002369:Castration; D003001:Cloning, Molecular; D004247:DNA; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D008297:Male; D009693:Nucleic Acid Hybridization; D049488:Phosphatidylethanolamine Binding Protein; D011467:Prostate / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D060789:Prostatein; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D060765:Secretoglobins; D013739:Testosterone / Q000494:pharmacology*; D014598:Uteroglobin; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D004247:DNA; D004274:DNA, Recombinant; D049488:Phosphatidylethanolamine Binding Protein; D060789:Prostatein; D012333:RNA, Messenger; D060765:Secretoglobins; D013739:Testosterone; D014598:Uteroglobin; C511482:PEBP1 protein, human; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B