Colocalization of oxytocin and neurophysin-I/II and of vasopressin and neurophysin-III in neurons of the sheep hypothalamus. An immunohistochemical study.
Antisera raised against, oxytocin, vasopressin and ovine neurophysins-I, -II and -III (oN-I, oN-II and oN-III respectively) were used in association with the immunohistochemical procedure to stain neurons of the formalin-fixed sheep hypothalamus. Cells that stain for oxytocin also contain oN-I and oN-II and differ from those that stain for vasopressin and oN-III. The observations support our earlier findings in the sheep that oxytocin- and vasopressin-related events are accompanied by the release of oN-I/II and oN-III respectively.
D000818:Animals; D006651:Histocytochemistry; D007031:Hypothalamus / Q000032:analysis* / Q000166:cytology; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000032:analysis*; D009481:Neurophysins / Q000032:analysis*; D010121:Oxytocin / Q000032:analysis*; D012756:Sheep; D014018:Tissue Distribution; D014667:Vasopressins / Q000032:analysis*
D009481:Neurophysins; D014667:Vasopressins; D010121:Oxytocin