A method for determination of immunoreactive trypsin in complex with alpha 1-antitrypsin in human sera.
A double antibody solid phase radioimmunoassay for the determination of cationic trypsin in complex with alpha 1-antitrypsin in human sera is described. No immunoreactive trypsin in complex with alpha 1-antitrypsin can be detected in normal sera. In sera from 8 patients with acute pancreatitis levels between 100 and 1000 micrograms/l are seen. The amount of trypsin in complex with alpha 1-antitrypsin in serum in acute pancreatitis equals or exceeds the amount of trypsinogen. Serum levels of trypsin in complex with alpha 1-antitrypsin also remain elevated longer than the trypsinogen levels in acute pancreatitis.
D000208:Acute Disease; D002850:Chromatography, Gel; D006801:Humans; D010195:Pancreatitis / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods*; D014357:Trypsin / Q000097:blood*; D014362:Trypsinogen / Q000097:blood; D000515:alpha 1-Antitrypsin / Q000097:blood*
D000515:alpha 1-Antitrypsin; D014362:Trypsinogen; D014357:Trypsin