Cloning of human hybridoma, myeloma and lymphoma cell lines using enriched human monocytes as feeder layer.
Human monocytes were prepared from peripheral blood by buoyant density centrifugation and subsequent absorption-elution on a column of gelatin beads. The eluted fraction containing 60-80% monocytes was used as feeder layer in cloning of the human lymphoma line RH-L4, the human myeloma line SKO-007, and a human hybridoma cell derived from the latter line. Cloning efficiencies were high in both liquid and semisolid media with all 3 cell lines tested. Feeder monocytes could also be successfully used after having been stored in liquid nitrogen.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000502:physiology; D006801:Humans; D006825:Hybridomas / Q000473:pathology*; D007158:Immunologic Techniques; D008223:Lymphoma / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology*; D009101:Multiple Myeloma / Q000473:pathology*; D051381:Rats; D011920:Rats, Inbred WF