title: Peptidyltransferase center of ribosomes. On the mechanism of action of alkaloid lycorine.
abstract: The molecular mechanism of action of the alkaloid lycorine has been revised. According to our results, lycorine inhibits the binding of CACCA-Leu comes from Ac to the donor site of the peptidyltransferase center of wheat-germ ribosomes, whereas the transpeptidation reaction in the system with the minimal model donor is not inhibited. The equilibrium constant of CACCA-Leu comes from Ac to the donor site of 80 S ribosomes is measured.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D047151:Amaryllidaceae Alkaloids; D004926:Escherichia coli / Q000201:enzymology; D010458:Peptidyl Transferases / Q000378:metabolism*; D010617:Phenanthridines / Q000494:pharmacology*; D010944:Plants / Q000201:enzymology; D011485:Protein Binding; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000201:enzymology*; D013045:Species Specificity; D014908:Triticum / Q000201:enzymology
chemical_list: D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; D012346:RNA, Transfer, Amino Acyl; D000217:Acyltransferases; D010458:Peptidyl Transferases; C015330:lycorine