Partial characterization of bovine leukemic cell populations.
Peripheral blood lymphocytes (LL) from cows with chronic lymphocytic leukemia (CLL) have been studied using a number of surface markers. Cell populations were obtained by partial enrichment and depletion using Sephadex G-200-anti-F(ab')2 and Sephadex G-200-anti-LL-Ig immunoadsorbent columns. It was shown that cell populations having different markers, i.e., surface immunoglobulins (sIg) and leukemia-associated antigens (LAA), are characterized by similar parameters. Thus the adherent cells obtained by both fractionation methods formed 1.1-4.0% of rosettes with sheep red blood cells, while 90.6-94.3% were sIg positive cells. The cytotoxicity test (CTT) performed with anti-B and anti-LL sera indicated that a vast majority of adherent cells reacted to the sera. Thus the adherent cells represent a population with a high percentage of B-cells.
D000818:Animals; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000662:veterinary*; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D011947:Receptors, Antigen, B-Cell