title: The Escherichia coli uvrD gene is inducible by DNA damage.
abstract: The product of the uvrD gene of Escherichia coli is involved in the repair of DNA damage, mismatch repair, and recombination. Phage Mud(Amp, Lac) was used to form a uvrD-lacZ fusion allowing uvrD expression to be followed by measuring the activity of beta-galactosidase, the product of the lacZ gene. uvrD expression was inducible by DNA damage and was under the control of lexA-recA regulatory system. Mutations in the uvrD gene that result in different phenotypes in respect to DNA repair and spontaneous mutation have been previously found. The phenotype of the uvrD::Mud(Amp, Lac) mutant was mutator and UV-sensitive but not as deficient in host cell reactivation or repair of methyl methanesulfonate damage as the previously described uvrD3 mutant.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D012697:Serine Endopeptidases; D014466:Ultraviolet Rays; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein