title: Experimental rabies in skunks: mechanisms of infection of the salivary glands.
abstract: Striped skunks (Mephitis mephitis) were inoculated into the right submandibular salivary gland with street rabies virus. They were killed at various times after inoculation and several tissues were examined by immunofluorescence and light microscopy. Right and left superior cervical, nodose and trigeminal ganglia, medulla oblongata and at least three regions of right and left submandibular salivary glands were examined by the fluorescent antibody technique. Intracerebral titrations of salivary gland suspensions were made in weanling white Swiss mice. Immunofluorescent material (inoculum) was detected in septa and connective tissue surrounding secretory units of the right submandibular gland immediately after inoculation, but otherwise antigen was not detected in either right or left submandibular glands without coincident antigen in the medulla oblongata. This occurred first on day 12 in areas of the gland remote from the inoculation site. Titers of virus were low at this time. Serum neutralizing antibodies occurred by day 7 in a few skunks. The time of development and distribution of antigen strongly suggest that, even after direct inoculation, neural networks are necessary for development of widespread infection of the salivary gland. The early occurrence of serum neutralizing antibodies in some of the skunks suggests that the immune response was activated by virus in the inoculum since immunofluorescence was not detected in any tissue at this time.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002335:Carnivora; D005260:Female; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000276:immunology; D008297:Male; D008526:Medulla Oblongata / Q000276:immunology; D012889:Mephitidae; D051379:Mice; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D011820:Rabies virus / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology; D013363:Submandibular Gland / Q000276:immunology / Q000382:microbiology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral