[Isolation and characterization of the basic forms of cytochrome P-450 from liver microsomes of C57BL mice after phenobarbital and 3-methylcholanthrene induction].
Using hydrophobic and ion-exchange chromatography, cytochromes P-450 and P-448 from liver microsomes of C 57 BL mice induced by phenobarbital and 3-methylcholantrene were isolated. The cytochromes purified to homogeneity as evidenced from SDS polyacrylamide gel electrophoresis were characterized in terms of molecular weight and catalytic and spectral properties and by peptide mapping. Cytochrome P-450, in contrast to cytochrome P-448, was not bound to the ion-exchanger and was eluted in a void volume. Cytochrome P-450 (Mr = 51 000) elicits a low spin signal and reveals a high catalytic activity toward aminopyrine and a low catalytic activity toward benz(a)pyrene. Cytochrome P-448 (Mr = 55 000) elicits both high an low spin signals and reveals a high catalytic activity toward benz(a)pyrene and a low catalytic activity toward aminopyrine. Limited proteolysis with papain demonstrated the differences in the proteins primary structure.
D000632:Aminopyrine; D000818:Animals; D001564:Benzo(a)pyrene; D001580:Benzopyrenes; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000302:isolation & purification*; D003580:Cytochromes / Q000096:biosynthesis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D010634:Phenobarbital / Q000494:pharmacology*; D013379:Substrate Specificity
D001580:Benzopyrenes; D003580:Cytochromes; D000632:Aminopyrine; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D010634:Phenobarbital
Vydelenie i kharakteristika osnovnykh form tsitokhroma P-450 iz mikrosom pecheni mysheĭ linii C57BL pri induktsii fenobarbitalom i 3-metilkholantrenom.