Spontaneous in vitro occurrence and long-term culture of murine B lymphoblast cell lines.
Under simple culture conditions, in vitro culture of T cell-depleted splenic mononuclear cells reliably promotes the outgrowth of B lymphoblasts. Such cells, termed BLY1, can be propagated as long-term B cell lines but are nontumorigenic in syngeneic recipients. The phenotype of BLy1 cells is unusual. They express certain B cell markers, including surface mu-lambda, I-Ak, and C3 and Fc receptors; however, delta heavy chain and kappa light chain production is absent. Although the cells do not express Thy-1 or Lyt-2, Lyt-1 is readily demonstrable. These distinctive features of BLy1 cells thus resemble a recently described B cell subset prominent early in ontogeny, and in the B cell defect of NZB mice.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D008213:Lymphocyte Activation; D016177:Macrophage-1 Antigen; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D010641:Phenotype; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D013997:Time Factors
D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M; D016177:Macrophage-1 Antigen; D011947:Receptors, Antigen, B-Cell; D011951:Receptors, Complement; D011961:Receptors, Fc