The liver in the IgA secretory immune system. Dogs, but not rats and rabbits, are suitable models for human studies.
The liver transport of polymeric IgA (pIgA) from plasma into bile and the immunohistochemical distribution of secretory component (SC) in the liver were studied in dogs, and compared to those in humans, rats, and rabbits. Results were as follows: (i) according to bile and serum protein concentrations and specific activities, plasma pIgA in dogs, like in humans, is transported into bile approximately 10 times more efficiently than albumin, as compared to 320 and 1060 times in rabbits and rats, respectively. (ii) Only approximately 1% of an i.v. dose of [125I]pIgA is transported into bile over 8 hr in dogs, like in humans, as compared to approximately 50% over 3 hr in rats and rabbits. These results agree with much smaller daily fractional catabolic rates of intravascular pIgA in dogs (0.28) and humans (0.48) than in rats (24.0). (iii) Total bile IgA contributes daily about 1.5 mg per kg to intestinal pIgA in dogs, a figure similar in humans (0.8 mg per kg) but much smaller than in rats (38 mg per kg) and rabbits (35 mg per kg). (iv) Biliary obstruction in dogs, like in humans, results only in minor and late increases in serum pIgA levels, contrasting with greater than 8-fold increases within 24 hr in rats and rabbits. (v) Unlike in rats and rabbits, SC in dog liver as well as in human liver cannot be detected in hepatocytes although clearly present in bile duct cells. To conclude: (i) major species differences in plasma-to-bile transport of pIgA exist, most probably related to species differences in the ability of hepatocytes to synthetize SC.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001646:Bile / Q000276:immunology* / Q000378:metabolism; D001692:Biological Transport; D001798:Blood Proteins / Q000378:metabolism; D002779:Cholestasis / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000378:metabolism*; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D008099:Liver / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D001798:Blood Proteins; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G