Studies on antibody repertoire: ontogenic development of mitogen-reactive B cells producing phosphorylcholine-specific and/or T15-associated 10/13 idiotope-positive antibodies.
Very high frequencies of phosphorylcholine-specific and 10/13 idiotope-positive precursor cells were observed in spleen and liver cells polyclonally stimulated by lipopolysaccharide in BALB/c mice. These frequencies do not show any significant difference at birth, in young and adult mice, suggesting that antibody molecules analyzed arose from a single germ line heavy chain variable region gene segment. The order of magnitude of these frequencies was comparable in germ-free mice and conventionally reared BALB/c mice. Therefore, external antigenic stimulation does not seem to be involved in the establishment of antibody specificity available repertoire.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D005856:Germ-Free Life; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology*
D007130:Immunoglobulin Idiotypes; D008070:Lipopolysaccharides; D010767:Phosphorylcholine; D002794:Choline