A C5-derived macrophage chemotactic factor from DNP-Ascaris extract-induced skin lesion in guinea pigs.
In complement-depleted condition induced by cobra venom factor (CoF), tissue macrophage reaction and macrophage chemotactic activity (MCA) in skin extract from skin lesions induced by DNP-Ascaris extract (DNP-As) were decreased, respectively. MCA in the fraction containing macrophage chemotactic factor b (B fraction) was decreased completely. While MCA in the fraction containing macrophage chemotactic factor a and c (A fraction) was affected only slightly. These results suggested that the decrease of MCA of B-fraction might be associated with depletion of complement system by CoF. Using anti-C5 antibody, it was confirmed that macrophage chemotactic factor-b (MCF-b) (MW 14,000: thermostable) might be derived from C5 fragment in vivo.
D000707:Anaphylaxis / Q000209:etiology; D000818:Animals; D001200:Ascaris / Q000276:immunology*; D002630:Chemotactic Factors / Q000276:immunology*; D002633:Chemotaxis; D002850:Chromatography, Gel; D003182:Complement C5 / Q000276:immunology*; D004140:Dinitrophenols / Q000276:immunology*; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology*; D007163:Immunosorbent Techniques; D008264:Macrophages; D008297:Male; D012867:Skin / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology*
D002630:Chemotactic Factors; D003182:Complement C5; D004140:Dinitrophenols; D006241:Haptens; D014020:Tissue Extracts