Selective binding of lipid A to responder and nonresponder B lymphocyte subpopulations.
125I-labeled lipid A is selectively bound to B cell-enriched populations. A combined method that employs autoradiography to detect binding of 125I-labeled lipid A and immunofluorescence to detect B cells is described. With this analytic technique it was demonstrated that 25%-30% of B cells in the spleen exhibit binding and that the binding by splenocytes from lipopolysaccharide responder mice cannot be distinguished from that of nonresponder mice.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D007457:Iodine Radioisotopes; D008050:Lipid A / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D007457:Iodine Radioisotopes; D008050:Lipid A