The preparation of stable phosphorylcholine-erythrocyte conjugates for use in plaque assays.
A method for the preparation of stable phosphorylcholine-conjugated erythrocytes is described. This method employs the formation of active esters of phosphorylcholine hydroxyphenylacetic acid (PC-HPA) and results in coupled red cells which are completely stable for 2-3 weeks. Using this procedure up to 80% of the activity was demonstrable after 6 weeks. Cells coupled with PC-HPA are suitable for the demonstration of anti-idiotype effects in a plaquing assay.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000921:Antibody-Producing Cells / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D004912:Erythrocytes / Q000378:metabolism*; D006462:Hemolytic Plaque Technique; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010648:Phenylacetates; D010767:Phosphorylcholine / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D010648:Phenylacetates; D010767:Phosphorylcholine; C008070:4-hydroxyphenylacetic acid; D002794:Choline