title: The effects of deuterium oxide (2H2O) on the polymerization of tubulin in vitro.
abstract: The initial rate and final extent of polymerization of both bovine brain tubulin and sea urchin egg tubulin were enhanced in the presence of 2H2O. The yields were increased in association with the elevation of the 2H2O concentration. 2H2O also reduced the critical concentration for polymerization of brain tubulin. Thermodynamic analysis was attempted using the temperature dependence of the critical concentration for polymerization in the presence of 2H2O. We obtained linear van 't Hoff plots and calculated thermodynamic parameters which were positive and were increased with the elevation of the 2H2O concentration. The enhancement of the polymerization of tubulin by 2H2O could, therefore, be the result of the strengthening of intra- and/or inter-molecular hydrophobic interactions of the tubulin molecules. We believe that the increase in length and number of microtubules of the mitotic spindles in the dividing cells of the eukaryotes with 2H2O may be caused by the direct involvement of 2H2O in the polymerization of tubulin.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D003903:Deuterium; D017666:Deuterium Oxide; D005260:Female; D007700:Kinetics; D046911:Macromolecular Substances; D010063:Ovum / Q000378:metabolism; D012617:Sea Urchins; D013816:Thermodynamics; D014404:Tubulin / Q000378:metabolism*; D014867:Water
chemical_list: D046911:Macromolecular Substances; D014404:Tubulin; D014867:Water; D003903:Deuterium; D017666:Deuterium Oxide