title: The complete amino acid sequence of yeast phosphoglycerate kinase.
abstract: The complete amino acid sequence of yeast phosphoglycerate kinase, comprising 415 residues, was determined. The sequence of residues 1-173 was deduced mainly from nucleotide sequence analysis of a series of overlapping fragments derived from the relevant portion of a 2.95-kilobase endonuclease-HindIII-digest fragment containing the yeast phosphoglycerate kinase gene. The sequence of residues 174-415 was deduced mainly from amino acid sequence analysis of three CNBr-cleavage fragments, and from peptides derived from these fragments after digestion by a number of proteolytic enzymes. Cleavage at the two tryptophan residues with o-iodosobenzoic acid was also used to isolate fragments suitable for amino acid sequence analysis. Determination of the complete sequence now allows a detailed interpretation of the existing high-resolution X-ray-crystallographic structure. The sequence -Ile-Ile-Gly-Gly-Gly- occurs twice in distant parts of the linear sequence (residues 232-236 and 367-371). Both these regions contribute to the nucleoside phosphate-binding site. A comparison of the sequence of yeast phosphoglycerate kinase reported here with the sequences of phosphoglycerate kinase from horse muscle and human erythrocytes shows that the yeast enzyme is 64% identical with the mammalian enzymes. The yeast has strikingly fewer methionine, cysteine and tryptophan residues.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004271:DNA, Fungal; D003854:Deoxyribonucleotides / Q000032:analysis; D005796:Genes; D006736:Horses; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D010735:Phosphoglycerate Kinase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D004271:DNA, Fungal; D003854:Deoxyribonucleotides; D010446:Peptide Fragments; D010735:Phosphoglycerate Kinase