title: Detection and significance of granulocyte alloimmunization in leukocyte transfusion therapy on neutropenic dogs.
abstract: Studies were carried out in dogs to detect alloimmunization to granulocytes by indirect immunofluorescence (GIIF) and to determine the significance of cross-matching in selecting donors for immunized recipients. All recipients were rendered neutropenic (less than 500 per microliter) by chemotherapy. Three groups of transfusions were evaluated. Group I consisted of 14 transfusions administered to sensitized dogs that were GIIF-negative with donor cells; Group II, 12 transfusions to the same dogs with GIIF-positive donor cells; Group III, 16 transfusions to nonimmunized control dogs. At 1 hour following transfusion, circulating granulocytes demonstrated increments of 956 +/- 137 per microliter (mean +/- SEM) in Group I dogs compared to 236 +/- 57 per microliter for Group II (p less than 0.001). Group III transfusions yielded increments of 888 +/- 116 per microliter, not significantly different from Group I. GIIF correlated better with transfusion results than granulocyte cytotoxicity or lymphocyte cytotoxicity tests or leukoagglutination cross-matching. Nineteen sera with alloantibodies to granulocytes were produced by random or intrafamilial immunizations with granulocytes. Partial characterization of specificities showed that granulocyte specificities were independent of DLA, and consistent with a genetic system of dominant alleles. It was concluded that a) alloimmunization to granulocytes is detected by the GIIF, b) positive tests predict transfusion effects, and c) a genetic system independent of DLA could be recognized on dog granulocytes.
mesh_terms: D000380:Agranulocytosis / Q000628:therapy*; D000818:Animals; D001787:Blood Group Incompatibility / Q000097:blood* / Q000175:diagnosis; D001788:Blood Grouping and Crossmatching; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology / Q000637:transplantation*; D006650:Histocompatibility Testing; D007519:Isoantigens / Q000032:analysis*; D008297:Male; D009503:Neutropenia / Q000628:therapy*
chemical_list: D007519:Isoantigens