title: Isolation of deuterated histones from yeast grown on media dissolved in 2H2O.
abstract: We have succeeded in growing Saccharomyces cerevisiae (baker's yeast) on media containing 2H2O and isolating the core histones highly deuterated in the non-exchangeable positions. The deuterated histones obtained here are of great value for their possible widespread use for structural studies of chromatin.
mesh_terms: D003903:Deuterium; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006657:Histones / Q000235:genetics / Q000302:isolation & purification*; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
chemical_list: D006657:Histones; D003903:Deuterium