Crossed-immunoelectrophoresis analysis of the urinary excretion of alpha 1-acid glycoprotein, alpha 1-antitrypsin, albumin, and transferrin in normal subjects and in patients with renal disease.
Urinary excretion of four plasma proteins having molecular weights between 44,100 and 90,000 daltons was studied by two-dimensional immunoelectrophoresis in normal individuals and in patients with different kinds of renal pathology. The proteins studied were: alpha 1-acid glycoprotein, 44,100 molecular weight (MW) and pH 2.7 isoelectric point (IP); alpha 1-antitrypsin, 54,000 MW and 4.0 IP; albumin, 69,000 MW and 4.9 IP; and transferrin, 90,000 MW and 5.6 IP. The proteins were measured in urine with an oligospecific serum produced by the immunization of rabbits with the 4S fraction obtained from normal human plasma by gel filtration on Sephadex G-200. The increase in urinary excretion of these proteins observed both among glomerulopathic and tubulopathic patients did not correlate with MW. Mean renal albumin excretion was 2.9 mg/24 h among normal individuals, 87.38 mg/24 h among patients with tubulopathy, and 3,228 mg/24 among patients with glomerulopathy. Among patients with glomerulopathy, there was a direct correlation between the increased excretion of these proteins and their IP, except for alpha 1-acid glycoprotein.
D058186:Acute Kidney Injury / Q000652:urine; D000293:Adolescent; D000328:Adult; D000419:Albuminuria; D005260:Female; D005921:Glomerulonephritis / Q000652:urine; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007674:Kidney Diseases / Q000652:urine*; D008297:Male; D008657:Metabolic Clearance Rate; D008875:Middle Aged; D009961:Orosomucoid / Q000652:urine*; D014168:Transferrin / Q000652:urine*; D000515:alpha 1-Antitrypsin / Q000652:urine*
D009961:Orosomucoid; D014168:Transferrin; D000515:alpha 1-Antitrypsin