title: The structure of the polysaccharide moiety of Rhodopseudomonas sphaeroides ATCC 17023 lipopolysaccharide.
abstract: The chemical structure of the polysaccharide moiety of the lipopolysaccharide Rhodopseudomonas sphaeroides ATCC 17023 was established. Mild acetic acid hydrolysis of isolated lipopolysaccharide, followed by preparative high-voltage paper electrophoresis afforded three oligosaccharides. They were characterized by chemical and physicochemical studies to be: GlcA(alpha 1----4)dOclA8P, Thr(6') GlcA(alpha 1----4)GlcA and GlcA(alpha 1----4)dOclA, where GlcA is D-glucuronic acid and dOc1A is 3-deoxy-D-manno-octulosonic acid. Carboxyl-reduction of the lipopolysaccharide followed by acid hydrolysis gave a trisaccharide: GlcA(alpha 1----4)Glc(alpha 1----4)Glc, showing the presence of three residues of glucuronic acids in the O-specific chain and indicating that only two of them are reducible by NaBH4. The linkage between the polysaccharide and lipid A was shown to be through a single 1,4-linked residue of dOc1A attached by a 2,6'-linkage to the lipid A moiety.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D003619:Dansyl Compounds; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis; D012242:Rhodobacter sphaeroides / Q000032:analysis*
chemical_list: D003619:Dansyl Compounds; D008070:Lipopolysaccharides; D009844:Oligosaccharides