The appearance of cytotoxic lymphocytes in unstimulated and concanavalin A-activated human peripheral mononuclear cells.
The presence of a mitogenic lectin during the cytotoxicity assay enables the detection of non-specific cytotoxicity (lectin-dependent cell-mediated cytotoxicity (LDCC]. Human mononuclear cells cultured at 37 degrees C in vitro became cytotoxic after 1 day, as detected by the LDCC. In some individuals, the presence of adherent cells during culture inhibited the development of cytotoxicity. Activation by concanavalin A (Con A) resulted in enhanced cytotoxicity after a few days in culture. There was no clear relationship between the lectin doses required for optimal induction of cytotoxicity and [3H]thymidine uptake. High Con A concentrations that induced a proliferative response often inhibited cytotoxicity in mononuclear cells depleted of adherent cells.
D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*
D003208:Concanavalin A