Human X human hybridoma producing monoclonal antibody against autologous cervical carcinoma.
A stable human X human hybridoma, termed CLNH11, was produced by fusing UC 729-6, a 6-thioguanine-resistant human lymphoblastoid B-cell line, with lymphocytes obtained from a patient with squamous cell carcinoma of the cervix. CLNH11 could grow both in serum-containing and serum-free media, and produced a significantly higher titer of antibody in serum-free media. CLNH11 secreted a human monoclonal immunoglobulin G which bound to the autologous cervical carcinoma in both tissue sections and primary cultured cells, the cervical cancer cell lines Hela and CaSki, but not to normal fibroblasts.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002460:Cell Line; D003470:Culture Media; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G