A new radioenzymatic assay for histamine using purified histamine N-methyltransferase.
Radioenzymatic assays for histamine (Hm) have found wide application. However, these procedures may lack the sensitivity necessary to quantify Hm in certain biological samples, such as human plasma. Purification of histamine N-methyltransferase (HNMT) has permitted the development of a new and highly sensitive radioenzymatic assay for Hm. HNMT was purified by sequential ion exchange, hydrophobic and molecular exclusion chromatography. The use of purified HNMT in the Hm assay has allowed the inclusion of high specific activity tritiated S-adenosyl-L-methionine ([3H]SAME) and the development of a simplified solvent extraction product isolation procedure. This assay has a sensitivity of approximately 2 picograms and is specific for Hm. Hm was easily quantified in human plasma and was found to be 303 +/- 81 pg/ml (mean +/- SD) in 8 male subjects. Substantial blank reduction and increased product conversion occur when purified HNMT is utilized in the Hm radioenzymatic assay, thus, increasing the sensitivity and possibly improving the specificity of this procedure.
D000818:Animals; D006632:Histamine / Q000097:blood*; D006637:Histamine N-Methyltransferase / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000201:enzymology; D011865:Radioisotope Dilution Technique; D051381:Rats; D012436:S-Adenosylmethionine; D014316:Tritium
D014316:Tritium; D012436:S-Adenosylmethionine; D006632:Histamine; D006637:Histamine N-Methyltransferase