title: [Difference in influenza virus strains in the intensity of the proteolytic modification of the base nucleocapsid protein NP56 to NP53 in infected cells].
abstract: Proteolytic cleavage of NP56 leads to NP53 protein of various influenza virus strains of human, avian, and animal origin in tissue culture was studied. A considerable portion of NP virus protein synthesized in human influenza virus-infected cells was modified by cellular proteases, and as early as 10 hours postinfection both intact (NP56) and cleaved (NP53) protein could be found in the cells. No proteolytic modification of NP protein was demonstrated in cells infected with 11 avian and animal influenza virus strains under study. Even 24 hours postinfection the cells contained intact (NP56) protein alone. Different resistance of NP protein of virus strains to cellular proteases allows the phenomenon of proteolytic modification of the nucleocapsid protein NP56 leads to NP53 to be used as a genetic marker of influenza virus strains.
mesh_terms: D000818:Animals; D002213:Capsid / Q000032:analysis / Q000378:metabolism*; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000378:metabolism*; D009976:Orthomyxoviridae Infections / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014776:Virus Cultivation
chemical_list: D014764:Viral Proteins; D010447:Peptide Hydrolases
vernacular_title: Razlichie shtammov virusa grippa po intensivnosti proteoliticheskoĭ modifikatsii osnovnogo nukleokapsidnogo belka NP56 K NP53 v zarazhennykh kletkakh.