Beta-cell tropin: synthesis and biological activity.
The structure of beta-cell tropin, an insulin secretagogue released by the neuro-intermediate lobe of the obese (ob/ob) mouse, has recently been determined as the 22-39 moiety of ACTH. A method for the preparation of this octadecapeptide using mild solid-phase procedures followed by preparative high pressure liquid chromatography is described. The molecular weight of the synthetic peptide has been confirmed by Fast Atom Bombardment mass spectrometry. Synthetic beta-cell tropin is indistinguishable in its chromatographic, antigenic and biological properties from natural beta-cell tropin.
D000324:Adrenocorticotropic Hormone; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000494:pharmacology; D010907:Pituitary Hormones / Q000138:chemical synthesis* / Q000494:pharmacology; D051381:Rats; D014357:Trypsin
D007202:Indicators and Reagents; D007328:Insulin; D010446:Peptide Fragments; D010907:Pituitary Hormones; C026834:beta-cell tropin; D000324:Adrenocorticotropic Hormone; D014357:Trypsin