Effect of subclinical levels of T-2 toxin on the bovine cellular immune system.
The effect of subclinical levels of mycotoxin T-2 on the cells of the bovine immune system was investigated in two in vivo experiments. In experiment 1, five calves were orally dosed with 0.3 mg/kg/day of T-2 toxin for 56 days and five calves were pair fed controls. The neutrophil function as measured by nitroblue tetrazolium reduction was reduced in the mycotoxin treated calves. The cutaneous reaction to intradermally injected phytohemagglutinin was reduced in the T-2 toxin treated calves. B-cell (SIg+) numbers increased slightly, but T-cell (PNA+) numbers were not affected during the experimental period. In the second experiment, six calves were given 0.5 mg/kg/day T-2 toxin orally for 28 days and six calves were pair fed controls. B-cell numbers and the response of a B-cell enriched fraction to phytohemagglutinin increased after toxin administration. T-cell numbers and the response of a T-cell enriched fraction and the whole mononuclear cell population to phytohemagglutinin was reduced only on day 19 posttoxin administration. The in vitro (T-2 toxin) exposure of the mononuclear cell population, B-cell enriched, or T-cell enriched fraction reduced their lymphoblastic response to mitogens. A 50% reduction was induced by as little as 1.4 ng/mL of T-2 toxin.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007111:Immunity, Cellular / Q000187:drug effects*; D007958:Leukocyte Count / Q000662:veterinary; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D008934:Mitogens; D012717:Sesquiterpenes; D013605:T-2 Toxin