Analysis of cell-surface protein changes accompanying differentiation of HL-60 cells.
The cell-surface proteins of HL-60 human promyelocytic leukemia cells have been compared to those of normal human neutrophils. Proteins of HL-60 cells surface labeled with 125I differed markedly from those of normal neutrophils, as shown by immunoprecipitation and polyacrylamide electrophoresis. Differentiation of HL-60 cells by treatment with dimethylformamide, trans-retinoic acid, or 12-O-tetradecanoylphorbol acetate did not modify the predominant surface-labeled proteins of HL-60 cells to produce a pattern similar to that of normal, mature neutrophils. However, the agents did induce greater quantities of minor cell-surface proteins immunoprecipitated by hyperimmune anti-human neutrophil serum. These immunoprecipitated proteins resembled several of the surface-labeled polypeptides of normal human neutrophils.
D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000032:analysis / Q000503:physiopathology*; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D009504:Neutrophils / Q000032:analysis
D008565:Membrane Proteins; D009363:Neoplasm Proteins