Induction of fibrin-specific antibodies by immunization with synthetic peptides that correspond to amino termini of thrombin cleavage sites.
Fibrin-specific antibodies have been produced in rabbits which were immunized with synthetic peptides. Specificity against human fibrin monomer was achieved because the synthetic peptide haptens were derived from sites unique to fibrin as compared with fibrinogen. Two undecapeptides were chemically synthesized according to the amino acid sequence of the amino termini of human fibrin alpha- and beta-chains which are revealed by thrombin cleavage. Rabbits immunized with either an alpha- or beta-chain peptide conjugate produced anti-peptide sera which reacted with fibrin monomer. Following immunoadsorption of the rabbit sera with human fibrinogen-Sepharose, fibrin-specific antibodies were detectable by solid-phase radioimmunoassay that did not react with fibrinogen. Antisera elicited by clotted human fibrin contained antibodies that reacted with fibrin and fibrinogen when treated in a similar manner.
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D005337:Fibrin / Q000276:immunology*; D005340:Fibrinogen / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007163:Immunosorbent Techniques; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D013917:Thrombin / Q000276:immunology*
D006241:Haptens; D010455:Peptides; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin