Selective inactivation of the 3' to 5' exonuclease activity of Escherichia coli DNA polymerase I by heat.
Heat selectively inactivates the 3' to 5' exonuclease activity of E. coli DNA polymerase I, resulting in reduced dNTP turnover and lower fidelity of replication of homopolymer and natural DNA templates.
D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology*; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors*; D006358:Hot Temperature; D007700:Kinetics; D013698:Templates, Genetic
D004256:DNA Polymerase I; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V