title: Immunoperoxidase labeling of Streptococcus mutans for scanning electron microscopy.
abstract: The application of an immunoperoxidase method to labeling of Streptococcus mutans subsp. sobrinus for scanning electron microscopy is described. Bacteria from four separate cultures were fixed in 2.5% buffered glutaraldehyde solution. The specimens were treated in accordance with an indirect immunolabeling procedure using a rabbit anti-S. mutans subsp. sobrinus gamma-globulin. Labeled bacteria were equipped with a layer of small, distinct globules of horse-radish peroxidase reaction product. Bacteria exposed to control incubations were not equipped with this layer.
mesh_terms: D001431:Bacteriological Techniques; D007124:Immunoenzyme Techniques; D008855:Microscopy, Electron, Scanning; D013295:Streptococcus mutans / Q000648:ultrastructure*