A monoclonal anti-pan-T-cell antibody. In vitro and in vivo studies.
A murine monoclonal antihuman T cell antibody is described that is pan-T in that it reacts with all T cells, including those from thymus, but not with B cells or other cells, except for a small subpopulation of null cells. Biochemical analysis demonstrated that the antigen detected is a glycoprotein, Mr 50,000 daltons, in which sialic acid, alpha-fucose and alpha-mannose are the important terminal or subterminal carbohydrates. Further analysis indicated that the antigen is the E-rosette-forming-cell (ERFC) receptor or a closely associated structure. Three patients with renal allograft rejection were treated with the pan-T cell monoclonal antibody and, although the antibody was in excess and bound to circulating T cells, no lasting effect on T cell numbers or graft rejection was noted.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology* / Q000502:physiology; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D006084:Graft Rejection; D006801:Humans; D007116:Immunization, Passive; D007223:Infant; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008254:Macaca nemestrina; D051379:Mice; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D010447:Peptide Hydrolases