[Determination of the activity of a polynucleotide phosphorylase preparation from E. coli].
Thin-layer chromatography was employed to measure the activity of polynucleotide phosphorylase (PNP-ase) from E. coli during purification and in the resultant preparation. The TLC data were verified with respect to released inorganic orthophosphate and to 14C-AMP incorporation rate. It is concluded that TLC can be used to determine PNP-ase activity in crude extract, at various purification stages and in the final preparation, yielding correct results.
D002855:Chromatography, Thin Layer / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000032:analysis* / Q000302:isolation & purification
D011117:Polyribonucleotide Nucleotidyltransferase
Opredelenie aktivnosti preparata polinukleotidfosforilazy iz E. coli.