An advantageous method for detection of Fc-receptors and for studying Fc-receptor-mediated phagocytosis.
A method employing fluorescent 2-hydroxyethyl methacrylate copolymer particles with dinitrophenyl haptenic group and anti-haptenic murine monoclonal antibodies of different isotypes is described for tracing Fc-receptors and for studies of phagocytosis via these cell surface structures. The possibility of quenching the fluorescence of non-phagocytosed particles enables us to distinguish clearly and reliably between internalized and cell surface-bound particles.
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D005456:Fluorescent Dyes; D007158:Immunologic Techniques; D051379:Mice; D010587:Phagocytosis; D011961:Receptors, Fc / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D011961:Receptors, Fc