Simple method for preparation of antibody-enzyme conjugates for enzyme-linked immunosorbent assays.
Mixing suspensions of rabbit antibody for staphylococcal enterotoxin A and commercial anti-rabbit immunoglobulin G-alkaline phosphatase conjugate produced a complex conjugate suitable for use as the second antibody in a conventional enzyme-linked immunosorbent assay. The method was useful for detecting 1 ng of staphylococcal enterotoxin A per ml.
D000469:Alkaline Phosphatase / Q000276:immunology; D000818:Animals; D004768:Enterotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D011817:Rabbits
D004768:Enterotoxins; D007074:Immunoglobulin G; C026066:enterotoxin A, Staphylococcal; D000469:Alkaline Phosphatase