Posttranscriptional modulation of gene expression in cultured rat hepatocytes.
The maintenance of high levels of two liver-specific mRNAs in cultured hepatocytes was achieved in a serum-free hormonally defined cell culture medium. However, this maintenance of liver-specific mRNA levels did not correlate with the level of transcription of the genes but was apparently due to increased stabilization of the tissue-specific mRNAs. The mRNA stabilization did not occur in serum-supplemented medium. In both defined and serum-supplemented medium, actin and tubulin mRNAs were also greatly increased, in both cases predominantly if not entirely due to increased mRNA stability.
D000199:Actins / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005796:Genes; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000235:genetics; D014158:Transcription, Genetic; D014404:Tubulin / Q000235:genetics; D000515:alpha 1-Antitrypsin / Q000235:genetics
D000199:Actins; D003470:Culture Media; D012333:RNA, Messenger; D012709:Serum Albumin; D014404:Tubulin; D000515:alpha 1-Antitrypsin