Concomitant up-regulation of insulin binding and elevated heat sensitivity in serum depleted CHO cells.
When HA-1 CHO cells were cultured in media with a range of fetal calf serum (FCS) concentrations, they became increasingly heat sensitive at low (5%) serum levels. Heat sensitization occurred concomitantly with increased insulin binding capacity. Insulin binding capacity also became more heat sensitive. Thermotolerance induced by a mild treatment (10 min/45 degrees C) 12 hr prior to assay, caused marked heat resistance expressed both as cell survival or insulin binding and abolished differences in sensitivity between the serum adapted cell lines.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D005260:Female; D006358:Hot Temperature; D007328:Insulin / Q000378:metabolism*; D010053:Ovary; D011972:Receptor, Insulin / Q000378:metabolism*
D003470:Culture Media; D007328:Insulin; D011972:Receptor, Insulin