title: Immune complexes of IgG1 and IgG3 subclasses in human breast cancer sera detected by monoclonal antibodies in an indirect immunoenzymatic assay.
abstract: Circulating immune complexes (CIC) of the IgG class were detected in sera from 37 (35.58%) out of 104 of the preoperated breast cancer patients studied using a CIq solid-phase enzymatic assay. Sera of positive patients were then tested to identify the IgG subclasses which are part of the complexes with the aim of showing a better correlation between CIC and disease evolution. IgG1- and IgG3-CIC were indirectly detected by the above test using monoclonal antibodies against the mentioned subclasses. Curves obtained with aggregated IgG1 and/or IgG3 showed good sensitivity and specificity of the test. Complexes containing both IgG1 and IgG3 were present in sera from the majority of patients; however, the relative amounts of these subclasses varied from patient to patient.
mesh_terms: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology* / Q000601:surgery; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D008408:Mastectomy
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D015922:Complement C1q; D003166:Complement Activating Enzymes