Construction of a shuttle vector and expression in Streptomyces lividans of the sulfonamide-resistance gene derived from Escherichia coli plasmid pSAS1206.
We have constructed a hybrid plasmid using Streptomyces lividans plasmid p1J101 and Escherichia coli plasmid pSAS1206. This plasmid, designated pFSH102, is able to replicate in both hosts and the sulfonamide-resistance gene encoded by pSAS1206 is phenotypically expressed in S. lividans.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010641:Phenotype; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D013449:Sulfonamides / Q000633:toxicity*; D014158:Transcription, Genetic
D013449:Sulfonamides; D004262:DNA Restriction Enzymes