Purification of pregnancy-specific beta 1-glycoprotein with a monoclonal antibody immunoadsorbent.
Hybrid cell lines secreting monoclonal antibodies against pregnancy-specific beta 1-glycoprotein (SP1) were produced by fusion of a mouse myeloma cell line with spleen cells from BALB/c mice immunized with purified placental SP1. The clones were further grown intraperitoneally in mice, and the ascites fluids contained anti-SP1 antibodies in high titers. One of the monoclonal antibodies was coupled to cyanogen-bromide-activated Sepharose and used for affinity chromatography of SP1 using late pregnancy serum as starting material. The purification of SP1 by means of immunoadsorption utilizing monoclonal antibodies offers remarkable advantages compared with the purification procedures used so far.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins