Induction of maturation in different types of B-cell lymphomas in vitro with TPA and antibodies to surface immunoglobulin.
Cells from eight selected cases of human non-Hodgkin lymphomas of various histological types (lymphocytic, centrocytic, centrocytic/centroblastic, and immunocytomas) were stimulated in vitro with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and anti-immunoglobulins (anti-Ig) against the surface immunoglobulin (sIg) on the tumour cells. Six of these cases responded by intracellular Ig accumulation as measured by flow cytofluorometry and direct phenotypical change into immunoblasts/plasmablasts as detected by light microscopic immunocytochemistry. However, the response to TPA alone varied considerably from case to case. These findings suggest that many, if not all, B-cell subsets have the capacity to develop directly into Ig-synthesizing cells (immunoblasts and plasmablasts). However, conditions for eliciting such events may vary, depending on the phenotypical properties and differentiation stage.
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D001402:B-Lymphocytes; D002454:Cell Differentiation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008223:Lymphoma / Q000473:pathology / Q000503:physiopathology*; D010704:Phorbols / Q000494:pharmacology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000888:Antibodies, Anti-Idiotypic; D010704:Phorbols; D011947:Receptors, Antigen, B-Cell; D013755:Tetradecanoylphorbol Acetate