Evaluation of assays for the detection of antibodies to rubella. A report based on data from the College of American Pathologists Surveys of 1982.
Data from laboratories participating in the College of American Pathologists Surveys in 1982 provided information on the trends in testing for antibodies to rubella. Methods used by participants included: passive hemagglutination, 29%; latex agglutination card assay test, 25%; hemagglutination inhibition, 19%; enzyme immunosorbent assays, 13%; indirect fluorescent antibody assays, 11%; and radioimmunoassay, 3%. The results from these methods generally agreed well with the standard HI test, particularly for detection of immunity in negative and strongly positive samples. Laboratories should use caution, however, that the tests they use give satisfactory results with low titered sera. Compared to laboratories using the one to three-day-old chick cells with Heparin-MnCl2 for HI, laboratories using Human O Cells tended to get higher titers, and laboratories using Fixed Chick cell or Kaolin methods tended to get lower titers.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests / Q000592:standards; D006386:Hemagglutination Tests / Q000592:standards; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D010338:Pathology, Clinical / Q000592:standards*; D011786:Quality Control; D011863:Radioimmunoassay / Q000592:standards; D012409:Rubella / Q000175:diagnosis / Q000276:immunology*; D012955:Societies, Medical; D014481:United States
D000914:Antibodies, Viral