In vitro release of tritiated monoamines from rat CNS tissue by the neurotoxic compound 1-methyl-phenyl-tetrahydropyridine.
The effects of the neurotoxic compound, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (1-MPTP), on monoamine release was determined using slices of rat corpus striatum or cerebral cortex preloaded with [3H]dopamine or [3H]serotonin, respectively. 1-MPTP released both monoamines in a concentration-dependent manner. [3H]Dopamine release was unaffected by tetrodotoxin, the absence of Ca2+, and nicotinic or muscarinic cholinergic antagonists. Dopamine uptake inhibitors had no effect on release from normal striatal slices but appeared to attenuate 1-MPTP-induced release from striatal slices of reserpine-pretreated rats.
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014439:Tyramine / Q000494:pharmacology
D001679:Biogenic Amines; D011725:Pyridines; D012701:Serotonin; D013779:Tetrodotoxin; D004246:Dimethylphenylpiperazinium Iodide; D012110:Reserpine; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D004298:Dopamine; D014439:Tyramine