Thymus-independent immunogenicity in vitro of the divalent antigen DNP-polyethylene oxide.
Chemically simple and physically well-defined dinitrophenyl derivatives of polyethylene oxide (DNP-PEO) can be prepared in a wide range of forms and sizes. These materials were used to investigate the molecular basis of immunogenicity and the binding of the antigens to membrane-bound receptors. Both di- and multivalent DNP-PEO activate normal murine B lymphocytes to yield primary anti-DNP antibody response in vitro. The immunogenicity is dependent on the carrier chain length but independent of T cells. Responses comparable to those induced by DNP-conjugated polymerized flagellin are induced by divalent linear materials of medium molecular weights of about 60,000. A highly multivalent material is moderately immunogenic, but at much lower antigen doses than divalent materials. The carrier PEO does not affect B-cell responses to DNP-PEO or T-cell response to succinyl concanavalin A. Moreover, it shows no polyclonal mitogenicity at concentrations as high as 1 mg/ml. Studies of antigen binding to cell surface DNP receptors show that the strongly immunogenic materials of medium molecular weights have an appreciable tendency to bind bivalently and thus potentially to crosslink receptors. The binding of smaller, less immunogenic antigen appears predominantly monovalent.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000955:Antigens, T-Independent / Q000008:administration & dosage / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D003601:Cytotoxicity Tests, Immunologic; D004136:Dinitrobenzenes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006462:Hemolytic Plaque Technique; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D009578:Nitrobenzenes / Q000276:immunology*; D010527:Perissodactyla; D011092:Polyethylene Glycols / Q000276:immunology*; D012397:Rosette Formation
D000955:Antigens, T-Independent; D004136:Dinitrobenzenes; D046911:Macromolecular Substances; D009578:Nitrobenzenes; D011092:Polyethylene Glycols