The effects of anions on phosphoglycerate mutase.
Chloride (5-100 mM) can activate the phosphoglycerate mutase reaction (3-PG in equilibrium with 2-PG). I-, N0-3, or higher Cl- inhibit the mutase reaction while allowing mixing of isotope from [32P]3-PG into a large pool of 2,3-bisphosphoglycerate. p-Hydroxybenzoate inhibits without causing isotope dilution. A number of anions (p-hydroxybenzoate approximate equal to NO3- greater than Cl- greater than Tes greater than acetate) stimulate the P-glycolate activated 2,3-bisphosphoglycerate phosphatase reaction of this enzyme. The same anions inhibit competitively with P-glycolate. A kinetic analysis relates the anion effects to changes in rates of association and dissociation of substrates.
D019794:2,3-Diphosphoglycerate; D000818:Animals; D000838:Anions / Q000494:pharmacology*; D002384:Catalysis; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011817:Rabbits
D000838:Anions; D004163:Diphosphoglyceric Acids; C019794:bisphosphoglycerate phosphatase; D019794:2,3-Diphosphoglycerate; D010770:Phosphotransferases; D010744:Phosphoric Monoester Hydrolases; D010736:Phosphoglycerate Mutase