title: Production of interspecies T cell hybrids which retain differentiation specific surface antigens.
abstract: Genetic mapping of differentiation specific surface antigens has been hampered by difficulty in preparation of interspecies hybrid cells which continue to express differentiated functions. A method has been developed for production of interspecies T cell hybrids which continue to express T cell specific cell surface molecules. Hybrids were constructed from either the human leukemic cell line MOLT-4 or freshly isolated human peripheral blood T cells and the mouse T lymphoma line BW5147. Optimal fusion efficiency resulted with pre-treatment of the human parental line with phytohemagglutinin followed by hybridization with 40% polyethylene glycol and plating without thymocyte feeder layers. Immortalization of hybrid lines was accomplished through addition of rat T cell growth factor to cultures.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007527:Isoenzymes / Q000235:genetics; D007942:Leukemia, Experimental / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D007527:Isoenzymes