The effect of high-protein and high-carbohydrate diets on [125I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri).
[125I]iodoinsulin-binding studies in the presence of a concentration range of bovine insulin were conducted to establish specific insulin-binding levels in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri) reared on control, high-protein or high-carbohydrate diets. Negative co-operativity was observed and receptor concentrations and apparent dissociation constants established for each preparation. No differences of specific binding attributed to diet were detected in skeletal muscle plasma membrane preparations; however, the receptor concentration of isolated hepatocytes from high-carbohydrate-reared trout was increased. This contrasted to comparable mammalian studies.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004032:Diet; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D007328:Insulin / Q000031:analogs & derivatives* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D011485:Protein Binding; D011972:Receptor, Insulin / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D007328:Insulin; C008147:insulin, iodo-; D011972:Receptor, Insulin