Oxidation of two hydroxylated ochratoxin A metabolites by alcohol dehydrogenase.
(4R)-4-hydroxyochratoxin A, (4S)-4-hydroxyochratoxin A, and 10-hydroxyochratoxin A, all formed from ochratoxin A, were incubated with alcohol dehydrogenase in the presence of NAD. Only (4R)-4-hydroxyochratoxin A and 10-hydroxyochratoxin A acted as substrates for the enzyme. K(m) and turnover number for 10-hydroxyochratoxin A were 110 muM and 0.1 s(-1), respectively.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D006736:Horses; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009793:Ochratoxins / Q000378:metabolism*; D010084:Oxidation-Reduction
D009793:Ochratoxins; C025589:ochratoxin A; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase