Enhancement of macrophage survival and complement production by factors from cloned T cells.
The addition of supernatants of some cloned mouse T cell lines to guinea pig peritoneal cells in tissue culture increased the production of the second (C2) and fourth (C4) components of complement as well as the enzyme beta-glucuronidase. Enumeration of cell populations demonstrated a simultaneous increase in cell survival. Supernatants that augmented functions of the cultured cells were obtained after alloantigen stimulation of a T cell line of C57BL/6 origin termed L2, but supernatants from a variant cell line derived from L2 and termed L2V had no effect. A delay of as little as 24 h in the addition of L2 T cell factors at the start of the cultures markedly diminished the effects on the cultured cells that were ordinarily observed 1-2 weeks later. These experiments suggest that, in this xenogenic system, complement-enhancing activity is part of a general stimulation of macrophages by cloned T cell factors.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000096:biosynthesis*; D003470:Culture Media; D006168:Guinea Pigs; D008222:Lymphokines / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D003470:Culture Media; D008222:Lymphokines; D003165:Complement System Proteins