Actinomycin D-binding in vivo: active chromatin preferred.
Escherichia coli RNA polymerase and the endogenous engaged RNA polymerase I were used as specific probes to monitor the physiologically inactive and active nucleolar chromatin template function, respectively. Actinomycin D bound preferentially to the physiologically active regions of rat liver nucleolar chromatin in vivo.
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003609:Dactinomycin / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D008099:Liver / Q000648:ultrastructure; D008297:Male; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D002843:Chromatin; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I