The biological activity of 4-chloromethylbiphenyl, benzyl chloride and 4-hydroxymethylbiphenyl in 4 short-term tests for carcinogenicity. A report of an individual study in the UKEMS genotoxicity trial 1981.
4-Chloromethylbiphenyl (4CMB), benzyl chloride (BC) and 4-hydroxymethyl-biphenyl (4HMB) were tested for biological activity in the following assays: (i) the Salmonella/microsome assay; (ii) a bacterial 'fluctuation' assays; (iii) a DNA repair assay in Hela cells, and (iv) a mouse lymphoma mutation assay. 4CMB was active in assays (i), (ii) and (iii) but not in (iv); BC was active in assays (i), (ii), (iii) but not in (iv) while 4HMB was inactive in all assays. Where biological activity was seen this did not require addition of a liver S9 preparation. 4CMB was more active than BC in all the test systems in which a positive response was obtained. The implication of these results for a test battery approach to in vitro testing is discussed.
D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology*; D001711:Biotransformation; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002273:Carcinogens; D004260:DNA Repair; D006367:HeLa Cells; D051379:Mice; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
D001593:Benzyl Compounds; D001713:Biphenyl Compounds; D002273:Carcinogens; C033488:4-hydroxymethylbiphenyl; C033487:4-chloromethylbiphenyl; C021292:benzyl chloride