[Construction of vectors for eukaryotes on the basis of fragments of mitochondrial DNA of animals].
The Bam HI fragment of rat liver mtDNA was used for construction and analysis of the cloning vehicles for prokaryota and eukaryota. Transformation of mutant bacterial cells deficient in DNA polymerase I polA with recombinant pBR-mtBA DNA was shown previously. Now the transformation of bacterial cells with recombinant pmt Tn9 DNA was observed. The Bam HI-A fragment of animal mtDNA may be used as vehicle replicon not only in bacterial cells but also in yeasts. The recombinant molecule was constructed from plasmide DNA YJpI which contained yeast chromosomal LEU2 gene and Bam HI-A mtDNA fragment.
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D003001:Cloning, Molecular; D004256:DNA Polymerase I / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009154:Mutation; D010957:Plasmids; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013045:Species Specificity; D014169:Transformation, Bacterial; D014170:Transformation, Genetic
D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D004256:DNA Polymerase I
Konstruirovanie vektorov dlia éukariot na osnove fragmentov mitokhondrial'noĭ DNK zhivotnykh.