Physical map of SeMNPV baculovirus DNA: an AcMNPV genomic variant.
A physical map of the plaque-purified SeMNPV-25 baculovirus DNA was constructed with HindIII, EcoRI, PstI, SstII, BstEII, BamHI, KpnI, and SmaI by multiple-enzyme digestion and DNA-DNA hybridization. The orientation of the physical map was to the AcMNPV polyhedrin gene, and a total of 104 restriction sites were ordered. The genome size was 131.89 kilobase pairs (87.05 X 10(6) Da). The physical maps of SeMNPV-25 DNA and AcMNPV-E2 DNA were compared, and they were similar except in four regions. Since the differences between the physical maps of SeMNPV-25 and AcMNPV-E2 were reconciled, the BstEII and SstII fragments for AcMNPV-E2 were also ordered. In addition, reiterated sequences in the SeMNPV-25 genome were identified and located on the physical map.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D007304:Insect Viruses / Q000235:genetics*; D008970:Molecular Weight; D000077183:Occlusion Body Matrix Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
D004279:DNA, Viral; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D004262:DNA Restriction Enzymes