An ELISA method for quantitation of macrophage migration from agarose microdroplets.
A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus detected correlate well with the migration as measured by a capillary tube assay for MIF.
D000818:Animals; D002464:Cell Migration Inhibition; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D008263:Macrophage Migration-Inhibitory Factors / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D012685:Sepharose
D008263:Macrophage Migration-Inhibitory Factors; D012685:Sepharose