Expression of hepatitis B surface antigen gene in yeast.
The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 X 10(5) molecules per cell of immunologically active HBsAg polypeptide in phosphate-free medium. The HBsAg polypeptides produced in the yeast cells were assembled into 20- to 22-nm spherical or oval particles and were immunogenic.
D000135:Acid Phosphatase / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004790:Enzyme Induction; D005796:Genes; D005818:Genetic Engineering; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D009876:Operon; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics
D006514:Hepatitis B Surface Antigens; D000135:Acid Phosphatase