A precise and sensitive method for measurement of spinal cord blood flow.
Spinal cord blood flow can be accurately and rapidly measured in rabbits by intravenous injection of [14C]iodoantipyrine, collection of sequential arterial samples, and abrupt termination of blood flow to the spinal cord by severing the aorta with a previously implanted ligature. The radioactive tracer concentrations in arterial and spinal cord samples can be rapidly measured in a scintillation counter. The spinal cord blood flow can then be calculated from these blood and tissue tracer concentrations. This method is suitable for use in anesthetized or unanesthetized small animals.
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000097:blood; D002250:Carbon Radioisotopes; D007700:Kinetics; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D012039:Regional Blood Flow; D013116:Spinal Cord / Q000098:blood supply*
D002250:Carbon Radioisotopes; D000983:Antipyrine; C008135:iodoantipyrine