Competitive growth experiments with related pairs of tartrate-fermenting and tartrate-non-fermenting strains of Salmonella typhimurium: relevance to biotyping studies.
For each of the isomers of tartaric acid, meso- or d- or l-, a pair of strains of Salmonella typhimurium was obtained, the one, a naturally occurring, non-fermenting strain and the other a spontaneous, tartrate-fermenting mutant derived from it. For each of the pairs of strains, competitive mixed cultures were grown from inocula of the tartrate-fermenting and tartrate-non-fermenting strains in peptone medium without or with the appropriate tartrate isomer. In each experiment, small numbers of tartrate-fermenting bacteria outgrew small or large numbers of tartrate-non-fermenting bacteria in 24 hours in tartrate-containing but not in tartrate-free peptone medium. The results of the experiments are discussed with reference to the choice of the definitive time of reading for tartrate-utilisation tests in the biotyping of S. typhimurium.
D000818:Animals; D002417:Cattle / Q000382:microbiology; D002455:Cell Division; D002645:Chickens / Q000382:microbiology; D003470:Culture Media; D005285:Fermentation; D006801:Humans; D009154:Mutation; D012210:Rhamnose / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013237:Stereoisomerism; D013644:Tartrates / Q000378:metabolism*
D003470:Culture Media; D013644:Tartrates; D012210:Rhamnose; C029768:tartaric acid