title: Molecular genetic analysis of genes encoding the heavy chains of rabbit IgG.
abstract: We analyzed the genes which encode the heavy chain constant region of rabbit IgG molecules. Five DNA clones derived from the chromosomal region which spans the C gamma coding sequences were isolated from a recombinant phage library of rabbit liver DNA. Four of these clones can be grouped together by overlaps; together they represent 37 kb of genomic DNA, and contain one C gamma gene and one tentatively identified C epsilon gene. A second C gamma gene was identified which did not overlap with the group of four clones because of restriction site differences found in the flanking regions 5' to the C gamma genes. Nucleotide sequences of the two C gamma genes were identical. Comparisons of the restriction maps of the two cloned C gamma genomic regions suggest that they may represent allelic regions of chromosomes of two different heavy chain haplotypes with polymorphisms in the regions flanking the C gamma genes.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics; D005796:Genes; D005815:Genetic Code; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D011817:Rabbits
chemical_list: D004270:DNA, Circular; D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D004262:DNA Restriction Enzymes