[Method for concentrating marrow stem cells using the IBM 2991 washer. Necessary preparation before in vitro treatment of bone marrow by pharmacologic or immunologic means].
The technique using the IBM 2991 blood cell processor is an effective technique for the concentration of mononuclear cells from large volumes of bone marrow. The marrow cells are layered on to Ficoll Metrizoate using the IBM processing set. The mononuclear cells and CFU-GM recoveries are in close relationship with the hematocrit of the cell suspension processed. Twenty two bone marrows have been collected and purified according to this protocol. The mononuclear cell recovery is an average of 78,3% (range: 44-92%) and the CFU-GM recovery is in average of 67,5% (range: 40-89%). At the end of the procedure the cell viability is satisfying (97,1% +/- 1,7 are trypan blue negatives). When it is necessary to remove from the bone marrow collected either malignant cells prior autologous bone marrow graft or T lymphocytes in an attempt to prevent GVHD in allogeneic BMT, the purity of marrow cell suspension become a fundamental parameter.
D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002469:Cell Separation / Q000295:instrumentation*; D002470:Cell Survival; D006098:Granulocytes / Q000166:cytology; D006400:Hematocrit; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells; D006863:Hydrogen-Ion Concentration; D009000:Monocytes / Q000166:cytology; D010976:Platelet Count
Méthode de concentration des cellules-souches médullaires utilisant le laveur IBM 2991. Préalable essentiel avant traitement in vitro de la moelle osseuse par des moyens pharmacologiques ou immunologiques.