Isolation and characterization of human B lymphocyte enriched populations. I. Purification of B cells by immune rosette depletion.
In the present study we describe a technique for the preparation of a highly purified B cell population from peripheral blood mononuclear cells (PBMC) by immune rosette depletion of non-B cells utilizing monoclonal antibodies directed at T cells, monocytes and null cells. The non-rosetted population contains greater than 90% B cells after a single rosetting step. The technique is simple, rapid and reproducible and results in minimal cell loss. In contrast, the E rosette negative (E-) fraction contains approximately 20% B cells whereas the surface immunoglobulin positive (sIg+ population obtained by Sephadex-anti-F(ab')2 column chromatography is comprised of approximately 60-80% B cells.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007140:Immunoglobulin Fab Fragments