A study of the induction of gene conversion in yeast by sodium bisulphite under radical reaction conditions.
Sodium bisulphite (NaHSO3) at low concentrations is known to undergo radical reactions in the presence of Mn2+ ions, generating sulphite anion radicals (SO-3.). The kinetics of pH variation in aqueous solutions of NaHSO3 in the presence of 0.1 mM-MnSO4 suggests that the anion radicals are generated rapidly at low bisulphite concentration (10 mM) and quenched at a higher concentration (100 mM). The ability of NaHSO3 to induce gene conversion in the diploid yeast Saccharomyces cerevisiae BZ34 under conditions suitable for radical reactions has been investigated. The results show that NaHSO3 does not induce gene conversion under these conditions.
D004305:Dose-Response Relationship, Drug; D005520:Food Preservatives / Q000494:pharmacology*; D005785:Gene Conversion / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009153:Mutagens / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D013447:Sulfites / Q000494:pharmacology*; D013997:Time Factors
D005520:Food Preservatives; D009153:Mutagens; D013447:Sulfites; C009279:sodium bisulfite