Effects of retinoids on the proliferation of murine sarcoma virus-transformed cells in serum-free culture: potentiation of autocrine stimulation.
In a recent report, we described a growth stimulatory effect of vitamin A derivatives (retinoids) on murine sarcoma virus (MSV)-transformed cell lines when tested in a serum-free culture condition. These cell lines were presumed to proliferate via autostimulatory (autocrine) mechanism when serum was omitted from culture medium. In the present communication, we examined the effect of 6 synthetic derivatives on the serum-free proliferation of 2 MSV-transformed cell lines. We confirmed essentially the previous results and found that one of them (Ro 11-1430) was a very weak growth stimulator. Further evidence indicated that retinoid-mediated growth stimulator. was not the result of increased production of growth factors but the result of potentiation of autocrine stimulation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000378:metabolism*; D008297:Male; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D012176:Retinoids / Q000494:pharmacology*; D009053:Sarcoma Viruses, Murine; D013268:Stimulation, Chemical; D013737:Testis
D003470:Culture Media; D006133:Growth Substances; D008934:Mitogens; D012176:Retinoids; D004247:DNA