title: Functional and morphological changes in experimental endolymphatic hydrops.
abstract: Electrocochleograms (ECochG) in response to tone bursts and electronystagmograms (ENG) during sinusoidal rotational stimulation were monitored in guinea pigs equipped with chronically implanted round-window and periocular electrodes before and for as long as 90 days after uni- or bilateral surgical obliteration of the endolymphatic duct. The presence of cochlear and saccular endolymphatic hydrops was verified during microdissection of the inner ear, and cochlear hair cell counts were performed in some of the animals. The results indicate that in surgically-induced endolymphatic hydrops, cochlear and vestibular changes occur similar to those observed clinically in Menière's disease: fluctuant threshold shifts, threshold elevation at low frequencies, sensorineural loss at all frequencies with recruitment, increase in the ratio of summating potential to compound action potential (SP/CAP), asymmetry and recruitment in the ENG, and increased vestibular excitability, with occasional post-rotational and spontaneous nystagmus. Functional changes could not be clearly correlated with those seen by light microscopy, since hair cell loss was confined mainly to the apical coil. The observations indicate that the guinea pig with surgically-induced endolymphatic hydrops can be a useful model of Menière's disease, not only on morphological grounds, but also on the basis of functional changes, which over the relatively short term of observation represent a dysfunction rather than a loss of sensorineural structures.
mesh_terms: D000818:Animals; D003051:Cochlea / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004567:Electrodes, Implanted; D004584:Electronystagmography; D004711:Endolymphatic Duct / Q000473:pathology; D005072:Evoked Potentials, Auditory; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000473:pathology; D008575:Meniere Disease / Q000473:pathology / Q000503:physiopathology*; D012399:Rotation; D014722:Vestibule, Labyrinth / Q000503:physiopathology