Inhibition of cytotoxicity for screening a monoclonal antibody to HLA antigen. Preparation of a highly specific monoclonal antibody to HLA-A2 antigen.
Cytotoxicity inhibition assay was established for the screening of a monoclonal antibody to HLA antigen. The assay involved the inhibition of typing cells with hybridoma culture supernatant and with F(ab')2 fragment of sheep anti-mouse IgG. Using the assay and the conventional microcytotoxicity test, an anti-HLA-A2 monoclonal antibody was screened.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D006801:Humans; D008722:Methods
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015789:HLA-A2 Antigen