Inhibition of bud initiation by ethyl N-phenylcarbamate results in meiotic development in the yeast Saccharomyces cerevisiae.
When diploid cells of Saccharomyces cerevisiae were incubated in acetate growth media containing 2.5 mM ethyl N-phenylcarbamate (EPC), bud initiation was inhibited preferentially, and eventually overgrown, unbudded cells accumulated. During subsequent incubation, meiosis and ascospore formation occurred at high frequencies. The behavior of EPC-treated cells was essentially the same as that of cells transferred to a starvation sporulation medium. EPC thus has a pronounced effect on the mitotic growth of yeast cells, which leads to meiotic development. Our observations indicate that EPC has a decisive function in the initiation of meiosis in rich growth media.
D000641:Ammonia / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology*; D002244:Carbon / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects*; D048448:Phenylcarbamates; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects*; D014520:Urethane
D002219:Carbamates; D048448:Phenylcarbamates; D014520:Urethane; D002244:Carbon; D000641:Ammonia; D005947:Glucose; C008866:phenylurethane