title: Phylogenesis of lymphocyte diversity. II. Characterization of Agama stellio Ig-negative lymphocytes by a heterologous anti-thymocyte serum.
abstract: A specific antiserum was raised in rabbits against thymocytes of the lizard Agama stellio (ATS). In indirect immunofluorescent (IF) assays, the in vitro absorbed ATS was, at any given dilution, reactable with thymocytes greater than peripheral blood lymphocytes (PBL) greater than or equal to splenocytes greater than bone marrow lymphocytes (BML). In quantitative absorption analyses the specificity of ATS for recognizing antigenic determinant(s) specific to Agama thymocytes (ASTA) and shared by other lymphocytes of probable thymic origin was indicated. In view of the inverse correlation of ASTA-bearing lymphocytes with Ig-bearing lymphocytes in lymphoid tissues, and the uniformity of results obtained with either an antiserum raised with Ig-negative thymocytes or antisera absorbed with purified gamma-fractions, ASTA may be a strict phenotype specific to Ig-negative thymocytes and other Ig-negative lymphocytes in the periphery. We propose a dichotomy of the T- and B-like arms of immunity in Agama, on the basis of presence or absence of ASTA.
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D008116:Lizards / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000961:Antilymphocyte Serum; D011947:Receptors, Antigen, B-Cell