title: N-methyl-N'-nitro-N-nitrosoguanidine induced genetic change during the meiotic cell cycle in Saccharomyces cerevisiae: an absence of S-phase specificity.
abstract: In the yeast Saccharomyces cerevisiae enhanced nuclear mutagenesis was found during meiotic DNA synthesis after treatment with N-methyl-N'-nitrosoguanidine (MNNG), but not during meiotic DNA synthesis. Further experiments found no significant variation in the mol. wt. of DNA from control or MNNG-treated samples during meiosis after alkaline sucrose gradient analysis. Using tritiated MNNG no variation in uptake of MNNG by meiotic cells was found, but enhanced binding of label from [3H]MNNG to mitochondrial DNA was detected during meiotic DNA synthesis when mitochondrial DNA synthesis occurred. In contrast, no enhanced binding of label from [3H]MNNG to the nuclear DNA during meiotic DNA synthesis was found.
mesh_terms: D004261:DNA Replication / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000235:genetics; D007399:Interphase / Q000187:drug effects*; D007700:Kinetics; D008540:Meiosis / Q000187:drug effects*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
chemical_list: D004272:DNA, Mitochondrial; D008769:Methylnitronitrosoguanidine