Bidirectional measurement of hydrogenase activity by the pH-stat method.
The protons produced by the catalytic activity of hydrogenase in H2 evolution from dithionite-reduced methyl viologen or through benzyl viologen reduction by H2 gas are automatically titrated by a pH-stat device. This approach allows the measurement of hydrogenase activity and ensures the constancy of pH during the reaction in absence of buffers. Kinetic assays and pH and temperature-dependence experiments with Desulfovibrio gigas hydrogenase performed by this method basically confirm the results obtained with customary manometric assay.
D003901:Desulfovibrio / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase; D008722:Methods; D010088:Oxidoreductases / Q000032:analysis*
D010088:Oxidoreductases; D006864:Hydrogenase