[Morphofunctional preservation of islet cells of the guinea pig pancreas during subculture].
The authors studied the possibilities of long-term culture of islet cells of guinea-pig pancreas by passages. beta-cells of guinea-pig pancreas were cultured for 180 days and underwent 6 passages. Morphological and radioimmunological studies of long-term-viable cells of the pancreas have shown that regular passages enable the obtaining of a homogeneous population of beta-cells. During all the passages, the cells preserve the morphological and functional features of islet tissue.
D000818:Animals; D002478:Cells, Cultured; D006168:Guinea Pigs; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014021:Tissue Preservation
D007328:Insulin
Morfofunktsional'naia sokhrannost' ostrovkovykh kletok podzheludochnoĭ zhelezy morskoĭ svinki pri ikh subkul'tivirovanii.