title: Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli.
abstract: Recombinant DNA techniques were used to isolate the Rickettsia prowazekii citrate synthase gene on the plasmid vector pBR322 by functional complementation of a gltA mutation of Escherichia coli K-12. Analysis of citrate synthase activity in crude extracts revealed that the enzyme expressed in E. coli retains the regulatory control mechanisms characteristic of the rickettsial enzyme.
mesh_terms: D002950:Citrate (si)-Synthase / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005838:Genotype; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D010957:Plasmids; D012281:Rickettsia / Q000201:enzymology* / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases