Insulin increases entrance of 2-deoxy-D-[3H]glucose in isolated rat brain microvessels.
Insulin in a dose of 0.5 U ml-1 in the incubation medium did not change ATP and phosphocreatine contents in the isolated rat brain microvessels, when energy production was supported by 5.5 mmol l-1 glucose. However, the entrance of non-metabolizable glucose analogue, 2-deoxy-D-[3H]glucose into the microvessel cells was strongly enhanced, almost 18-fold.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000032:analysis; D002199:Capillary Permeability / Q000187:drug effects*; D003847:Deoxyglucose; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D010725:Phosphocreatine / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D005947:Glucose