Detection of specific immunoglobulin M antibody to different flaviviruses by use of enzyme-labeled antigens.
An enzyme immunoassay was developed for the detection of human immunoglobulin M (IgM) antibody to different flavivirus antigens. The IgM antibody of human sera was selectively bound to anti-IgM antibody-coated solid-phase plates. Flavivirus IgM antibodies were then detected by use of various enzyme-labeled antigens. The flavivirus antigens (dengue type 2 virus, West Nile virus, and tick-borne encephalitis virus) were produced in suckling mice. The antigens were labeled with horseradish peroxidase by adding the activated enzyme at alkaline pH to sucrose-acetone-treated antigens. Addition of unlabeled mouse brain suspension of uninfected animals to the diluted enzyme-labeled antigens effectively reduced nonspecific binding to the solid phase. In patients with acute flavivirus infections, viral IgM antibody could be demonstrated with high sensitivity. Furthermore, the enzyme-labeled antigen-IgM test showed greater specificity than the hemagglutination inhibition test.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D003429:Cross Reactions; D003715:Dengue / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology; D004675:Encephalitis, Tick-Borne / Q000276:immunology; D005416:Flavivirus / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D013045:Species Specificity; D014036:Togaviridae Infections / Q000276:immunology*; D014901:West Nile Fever / Q000276:immunology; D014902:West Nile virus / Q000276:immunology
D000914:Antibodies, Viral; D007075:Immunoglobulin M