A new method of testing anti-allergic drugs.
Rats were sensitized with a single intraperitoneal dose of bovine serum albumin in alhydrogel plus Bordetella pertussis vaccine, and local anaphylaxis was elicited in the paw by soluble antigen 2 weeks later. The response was mainly due to IgE-type antibodies and proved to be highly sensitive to beta-adrenoceptor agonists. Dexamethasone inhibited the response after a lag phase. Methysergide, disodium chromoglycate, diethylcarbamazine, BW 775/c, nordihydroguarjaretic acid, and FPL 55712 were also suppressive, while mepyramine was without effect. A synergism between methysergide and FPL 55712 was shown. Active local paw anaphylaxis appears to be adequate and easily applicable for large-scale screening of anti-allergic drugs.
D000818:Animals; D002927:Cimetidine / Q000008:administration & dosage; D003907:Dexamethasone / Q000008:administration & dosage; D004837:Epinephrine / Q000008:administration & dosage; D005260:Female; D006969:Hypersensitivity, Immediate / Q000188:drug therapy*; D007158:Immunologic Techniques; D007545:Isoproterenol / Q000008:administration & dosage; D008297:Male; D008784:Methysergide / Q000008:administration & dosage; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
D003907:Dexamethasone; D002927:Cimetidine; D007545:Isoproterenol; D008784:Methysergide; D004837:Epinephrine