Characterization of monoclonal antibodies that define rat T cell alloantigens.
A panel of monoclonal antibodies with specificity for rat T cell alloantigens was prepared by somatic cell hybridization with the use of spleen cells from either BH (ART-1b, 2b) or NBR (ART-1b, 2a) rats immunized with LEW (ART-1a, 2a) thymus, spleen, or lymph node cells. Comparison of the antibodies resulted in three distinct patterns of complement-mediated cytotoxicity. Further comparison of four representative hybridoma antibodies with antisera having specificity for ART antigens by indirect immunofluorescence demonstrated that two of the three patterns corresponded to anti-ART-1a and anti-ART-2a specificities. The patterns of binding of the anti-ART hybridomas were shown to be different from those produced by mouse monoclonal antibodies having anti-leukocyte-common (L-C) or anti-Thy-1.1 activities as well as antibodies produced by the monoclonal W3/25, which detect a subpopulation of T lymphocytes. Functional studies performed in vitro with cells fractionated by treatment with the monoclonal anti-ART antibodies and complement showed the ART-1 determinant recognized by the hybridoma products BC 84A or BY 12.3 is expressed by mature cytolytic T lymphocytes (CTL) and by cells involved in the generation of CTL during MLR, but not plaque-forming cells. Together, the data suggest the antibodies BC 84A and BY 12.3 can be used to define rat T cell populations. To date, the functional significance of cell populations recognized by monoclonal antibodies having anti-ART-2a or anti-ART-3-like activities has not been determined.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000921:Antibody-Producing Cells / Q000276:immunology; D000961:Antilymphocyte Serum / Q000032:analysis; D001666:Binding Sites, Antibody; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006462:Hemolytic Plaque Technique; D006822:Hybrid Cells / Q000276:immunology; D007519:Isoantigens / Q000276:immunology*; D051381:Rats; D011912:Rats, Inbred ACI; D011915:Rats, Inbred BUF; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D007519:Isoantigens