Isolation and identification of an alpha 2 subclass lymphotoxin (LT) subunit from the high-molecular-weight (complex) human LT class.
The high-molecular-weight (MW) complex class (MW = greater than or equal to 200,000) of lymphotoxin (LT) may be involved in the process of lymphocyte-mediated cytolysis. This LT class was produced by concanavalin A-stimulated human adenoid and tonsil lymphocytes in vitro and purified approximately 1000 fold by a scheme employing lectin affinity chromatography on concanavalin A-Sepharose, negative hydrophobic affinity chromatography on phenyl-Sepharose, and molecular sieving on Ultrogel AcA 44. A cell-lytic subunit, termed C-alpha toxin, was dissociated from this partially purified complex LT (Cx) preparation. The identification of C-alpha toxin as an alpha 2 LT form was established by its comigration with the alpha 2 LT form during native polyacrylamide gel electrophoresis (PAGE), molecular sieving, and isoelectricfocusing. In addition, C-alpha toxin was neutralized by antiserum raised against purified alpha 2 LT, and the latter was neutralized by antiserum raised against C-alpha toxin. Furthermore, preliminary evidence indicates that both alpha 2 LT and C-alpha toxin are composed of peptides with apparent MW of approximately 69,000. Additional data indicate the Cx LT form is composed of alpha 2 LT and other subunits.
D000234:Adenoids / Q000276:immunology; D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003208:Concanavalin A; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007739:L Cells / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008233:Lymphotoxin-alpha / Q000302:isolation & purification*; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D014066:Palatine Tonsil / Q000276:immunology
D008233:Lymphotoxin-alpha; D046911:Macromolecular Substances; D003208:Concanavalin A