Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.
A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41% protein, 49% carbohydrate and 10% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.
D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D006023:Glycoproteins; D009844:Oligosaccharides / Q000032:analysis; D010750:Phosphoproteins; D015800:Protozoan Proteins; D011863:Radioimmunoassay; D014349:Trypanosoma cruzi / Q000032:analysis*
D000596:Amino Acids; D000606:Amino Sugars; C038790:GP72 antigen, Trypanosoma; D006023:Glycoproteins; D009844:Oligosaccharides; D010750:Phosphoproteins; D015800:Protozoan Proteins; D000117:Acetylglucosamine