Simultaneous detection of two cell surface antigens by a red blood cell rosette-microsphere binding method, and its application to the study of multiple myeloma.
A sensitive method for the simultaneous detection of 2 cell surface antigens is described. One antigen is defined by a monoclonal antibody which is subsequently detected by binding bovine red blood cells coated with a suitable antibody. The second antigen is detected by antibody coated, small, highly fluorescent microspheres. The cells can be counted under visible light, or under low intensity visible light together with UV light and no switching of the illumination or filter systems is required to distinguish positive and negative cells. The application of the procedure to finding myeloma idiotype positive B cells in the blood of patients with multiple myeloma is described.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D002417:Cattle; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D051379:Mice; D008863:Microspheres; D009101:Multiple Myeloma / Q000276:immunology*; D012397:Rosette Formation / Q000379:methods*
D000954:Antigens, Surface; D007130:Immunoglobulin Idiotypes