Paper replication method for isolation of radiation-sensitive mutants.
A filter paper replication system particularly useful for isolation of radiation-sensitive mutants of pigmented bacteria was devised. The fidelity of replication was high. Adhesion between a paper disk and a properly dried master plate provided adequate contact pressure. The replicas arising from this technique constitute a convenient apparatus for general application in isolation of clones sensitive to a discriminating treatment.
D001431:Bacteriological Techniques; D008837:Micrococcus / Q000235:genetics* / Q000302:isolation & purification / Q000528:radiation effects; D009154:Mutation; D014466:Ultraviolet Rays