Comparison of the microtitration agglutination test and the enzyme-linked immunosorbent assay for the detection of herds affected with swine dysentery.
A method was designed to evaluate and compare the microtitration agglutination test (MAT) and the enzyme-linked immunosorbent assay (ELISA) to detect antibodies in swine sera to Treponema hyodysenteriae and thereby establish a method for determining the prevalence of swine dysentery (SD) in herds. According to sampling criteria based on the hypergeometric distribution, sera were collected from 3 age groups of swine from farms having a history of SD on the premises (SD+) recently or being free of the disease (SD-). The highest degree of test sensitivity was obtained when sera from market age swine were evaluated with the ELISA. Of 14 SD+ herds from which sera were obtained from market-age swine, 13 were positive with the ELISA (93%); none of the 8 SD- herds was positive. The detection rates of individual swine in the SD+ herds for the 2 tests by age group were as follows: MAT--adult swine 1.4%, market-age swine 6%, and weaned pigs 0.8%; ELISA--adult swine 16%, market swine 31%, and weaned pigs 0.5%.
D000367:Age Factors; D000372:Agglutination Tests / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D004403:Dysentery / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014208:Treponema / Q000276:immunology; D014211:Treponemal Infections / Q000175:diagnosis / Q000662:veterinary*
D000907:Antibodies, Bacterial