title: Relationship between cytotoxicity and DNA damage in mammalian cells treated with anthracenedione derivatives.
abstract: The effects of two anthracenedione derivatives on in vitro cell survival and DNA of Chinese hamster ovary (CHO) cells were investigated. The two drugs studied were 1,4-dihydroxy-5,8-bis-(2-[2-hydroxyethyl)amino)ethylamino)-9,10-anthracenedione (DHAQ, NSC No. 279836) and 1,4-bis-(2-[2-hydroxyethyl)-amino)ethylamino)-9,10-anthracenedione (HAQ, NSC No. 287513). DHAQ was 100-fold more potent in reducing cell survival than HAQ. DNA strand breaks were assayed by alkaline elution. DHAQ (10 ng/ml) caused more strand breakage than 1000 ng/ml HAQ. This difference correlates well with their differences in ability to kill cells.
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000502:physiology; D019286:Endopeptidase K; D010450:Endopeptidases; D005260:Female; D008942:Mitoxantrone / Q000031:analogs & derivatives; D010053:Ovary
chemical_list: D000880:Anthraquinones; D004268:DNA-Binding Proteins; D008942:Mitoxantrone; D010450:Endopeptidases; D019286:Endopeptidase K; C020182:ametantrone