Simple rapid methods for freezing hybridomas in 96-well microculture plates.
Macroscopic hybridoma colonies were frozen and recovered in a good state of viability in 96-well microculture plates using 2 freezing procedures. These methods offer convenient and rapid means of preserving hybridomas and will permit laboratories developing monoclonal antibodies to distribute workloads to more manageable levels without discarding possibly valuable hybridomas.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D003584:Cytological Techniques; D005615:Freezing; D006825:Hybridomas / Q000166:cytology; D008722:Methods; D051379:Mice; D013997:Time Factors
D000911:Antibodies, Monoclonal