Transfer of plasmid RP1 into chemolithotrophic Thiobacillus neapolitanus.
RP1, a broad-host-range incompatibility group P1 plasmid specifying multiple drug resistances, has been transferred into the chemolithotrophic bacterium Thiobacillus neapolitanus. The ability of T. neapolitanus to receive, express, and transmit RP1-encoded antibiotic resistances was examined. The data show that this obligate chemolithotroph can accept, replicate, and express heterologous plasmid DNA from a heterotrophic bacterium.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D011815:R Factors; D013855:Thiobacillus / Q000187:drug effects / Q000235:genetics*
D000900:Anti-Bacterial Agents