title: Improved conversion of fumarate to succinate by Escherichia coli strains amplified for fumarate reductase.
abstract: Two recombinant plasmid Escherichia coli strains containing amplified fumarate reductase activity converted fumarate to succinate at significantly higher rates and yields than a wild-type E. coli strain. Glucose was required for the conversion of fumarate to succinate, and in the absence of glucose or in cultures with a low cell density, malate accumulated. Two-dimensional gel electrophoretic analysis of proteins from the recombinant DNA and wild-type strains showed that increased quantities of both large and small fumarate reductase subunits were expressed in the recombinant DNA strains.
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005650:Fumarates / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D008293:Malates / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010957:Plasmids; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid
chemical_list: D004274:DNA, Recombinant; D005650:Fumarates; D008293:Malates; D013386:Succinates; C030298:malic acid; C032005:fumaric acid; D019802:Succinic Acid; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020155:fumarate reductase (NADH); D005947:Glucose; D000645:Ammonium Sulfate