Fibronectin and anchorage-independent and anchorage-dependent growth of benign and malignant cell lines.
The presence of fibronectin in three "malignant" (AU-471, AU-436, LT-2) and two "benign" (BHK-21, WI-38) cell lines was demonstrated with a fluorescent antibody technique; two malignant (AU-471, AU-436) cell lines were fibronectin-negative and one (LT-2) retained fibronectin expression. One "benign" cell line (WI-38) expressed fibronectin, the other (BHK-21) did not. Anchorage-independent soft agar (AISA) growth correlated better with loss of fibronectin than with malignant potential. All three fibronectin-negative cell lines (benign and malignant) grew anchorage-independently (AU-471, AU-436, BHK-21), and both fibronectin-positive cell lines were anchorage-dependent (LT-2, WI-38). Surprisingly, the addition of Clg to anchorage-independent cells increased their anchorage-independent soft-agar cloning efficiency, but had no effect on anchorage-dependent cell lines. Anti-Clg antibodies decreased AISA growth. The effect of Clg on anchorage-independent growth varied with the concentration, and also between cell lines, and a variation in effect was noted between anchorage-independent (AISA) and anchorage-dependent (in flasks) growth even in the same cell line.
D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D009369:Neoplasms / Q000032:analysis* / Q000503:physiopathology; D010641:Phenotype
D005353:Fibronectins