Inhibition of the alpha-glucosidase specific for collagen disaccharide units in diabetic rat kidney by in vivo glucose levels: possible contribution to basement membrane thickening.
The activity of the alpha-glucosidase specific for collagen disaccharide units has been measured in kidney cortex homogenates of streptozotocin-diabetic rats under three different conditions: (1) in dialyzed homogenates; (2) in non-dialyzed homogenates; (3) in non-dialyzed homogenates to which glucose was added to compensate for dilution due to homogenization and to reach the glucose concentration determined in kidney cortex (37.5 +/- 2.8 mmol/kg diabetic cortex versus 6.8 +/- 0.3 mmol/kg normal cortex). Under the latter condition, the enzyme activity was markedly decreased in diabetic kidney cortex when compared with that of normal age-matched controls: 4.03 +/- 0.25 versus 6.82 +/- 0.29 units/mg protein (p less than 0.001). Inhibition of enzyme activity was also significant in non-dialyzed diabetic homogenates without additional glucose. In the absence of glucose (in the dialyzed homogenates), it is confirmed that the enzyme activity is elevated in diabetic kidney. The glucose inhibition of the enzyme activity has been shown to be important under in vivo conditions. It may therefore contribute to kidney basement membrane thickening.
D000818:Animals; D001485:Basement Membrane / Q000473:pathology; D003094:Collagen / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000473:pathology; D003956:Dialysis; D004187:Disaccharides / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D005959:Glucosidases / Q000037:antagonists & inhibitors*; D065089:Glycoside Hydrolase Inhibitors; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000520:alpha-Glucosidases
D004187:Disaccharides; D065089:Glycoside Hydrolase Inhibitors; D003094:Collagen; D005959:Glucosidases; C038130:glucosyl-galactosyl-hydroxylysine glucohydrolase; D000520:alpha-Glucosidases; D005947:Glucose