Translation in vitro and regulation of mouse liver S-adenosylmethionine synthetase messenger RNA.
Total RNA was isolated from adult mouse liver tissues. The alpha- and beta-form isozymes of S-adenosylmethionine synthetase existing in liver were synthesized in a reticulocyte lysate cell-free system under the direction of total RNA and were immunoprecipitated with antibody to the beta-form. The newly synthesized and the in vivo labeled S-adenosylmethionine synthetase subunits were compared by SDS-polyacrylamide gel electrophoresis. Both the alpha- and beta-forms consist of the same size Mr 48 000 subunit. The level of the beta-form mRNA activity in mouse liver was shown to increase following intraperitoneal transplantation of Ehrlich ascites tumor cells and the changes in the mRNA activity parallel those in the cellular level of S-adenosylmethionine synthetase beta.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008716:Methionine Adenosyltransferase / Q000096:biosynthesis* / Q000302:isolation & purification; D051379:Mice; D009368:Neoplasm Transplantation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014166:Transferases / Q000096:biosynthesis*
D007527:Isoenzymes; D012333:RNA, Messenger; D014166:Transferases; D008716:Methionine Adenosyltransferase