Hyperacute rejection of xenografts. I. Differences in affinity to ConA--Sepharose of IgG--fraction of the affluent and effluent blood.
The immunoglobulin fraction from affluent and effluent sera from the rabbit liver transplanted to a dog partially purified on ion exchange DEAE--Sephadox A-50 was chromatographed on ConA-Sepharose. The picture of SDS polyacrylamide slab gel electrophoresis (PAGE) showed in the xenograft serum a considerably higher intensity of bands corresponding to the mobility of heavy and light IgG chains. This suggested the presence of glycoproteins coupled to IgG. Immunoelectrophoresis proved occurrence in the transplant serum of at least six additional components precipitating with whole anti-dog serum. These components could be either glycoprotein antigens or complement components, free or bound to IgG subclasses.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004285:Dogs; D006023:Glycoproteins / Q000302:isolation & purification; D006084:Graft Rejection; D007074:Immunoglobulin G / Q000302:isolation & purification*; D016031:Liver Transplantation; D011817:Rabbits; D012685:Sepharose / Q000031:analogs & derivatives; D014183:Transplantation, Heterologous
D000936:Antigen-Antibody Complex; D006023:Glycoproteins; D007074:Immunoglobulin G; C028326:concanavalin A-sepharose; D012685:Sepharose