Changes in lysosomal enzyme activities of eye tissues in endotoxin-induced inflammation.
Lysosomal enzyme activities in rabbit ocular tissues were examined at various times after the intravitreal injection of endotoxin. Lysosomal enzyme activities in the aqueous, vitreous, and pigment epithelium-choroid were elevated 3 h after injection. It is deduced from the fluorescein-labeled dextran and SDS-polyacrylamide gel electrophoresis analysis of the aqueous, vitreous, and pigment epithelium-choroid from endotoxin injected rabbits that the elevation of lysosomal enzyme activities are probably due to a breakdown in blood-ocular barriers.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001192:Arylsulfatases / Q000032:analysis; D002401:Cathepsin B; D002403:Cathepsins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D009877:Endophthalmitis / Q000139:chemically induced / Q000201:enzymology*; D004731:Endotoxins; D005123:Eye / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D010539:Permeability; D011817:Rabbits
D004731:Endotoxins; D000135:Acid Phosphatase; D001192:Arylsulfatases; D005966:Glucuronidase; D002403:Cathepsins; D002401:Cathepsin B