[Effect of beta-adrenergic mimetics and histamine on the capacity of T-lymphocytes from asthmatic patients and healthy persons to form E-rosettes].
The active and total E-rosettes, according to Wybran et al. method were studied in 30 patients with various forms of bronchial asthma and 6 healthy subjects, and the reactivity of rosette--forming T-lymphocytes was determined after stimulation by asthmopent, histamine and acetylcholine. It was established that the lymphocyte histamine-sensitive adenylatecyclase shows a preserved response in patients with atopic asthma and via the formation of cAMP reduces the percentage of the "active E-EFC, whereas adrenaline-sensitive adenylatecyclase is with reduced activity in the same patients. The latter corresponds to the absence of essential changes in the percentage of E-RFC in the atopic asthmatics. To some of the patients, guanylatecyclase activity was studied prior to and past incubation with acetylcholine or histamine of the lymphocytes, treated not with colchicine, used as performing agent on the microtubular apparatus of the cell. To a group of healthy subjects, colchicine effect was determined at the level of cAMP. Conjugated correlations were established between cAMP and cGMP versus the microtubular apparatus and hence--as regards the rosette-formation.
D000109:Acetylcholine / Q000494:pharmacology; D001249:Asthma / Q000276:immunology*; D001993:Bronchodilator Agents / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects* / Q000276:immunology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count; D009930:Organic Chemicals; D012397:Rosette Formation; D013566:Sympathomimetics / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
C025738:Asthmopent; D001993:Bronchodilator Agents; D009930:Organic Chemicals; D013566:Sympathomimetics; D006632:Histamine; D000109:Acetylcholine; D003078:Colchicine
Efekt na beta-adrenergichnite mimetitsi i khistamina vŭrkhu kapatsiteta na T-limfotsitite ot astmatichno bolni i zdravi da obrazuvat E-rozetki.