[Antigenic detection and identification of Haemophilus influenzae and quantitative immunoelectrophoresis].
A one-step polyvalent rocket-line immunoelectrophoresis (PRLIE) for antigenic detection and identification of Haemophilus influenzae is described. A standard pattern of six lines corresponding to each individual antigen-antibody system has been established. The polyvalent antigen was an adequate mixture of each serotype antigen and the polyvalent antiserum was a pool of the six monospecific antiserum raised in rabbits (Institut Pasteur de Lyon). Each line has been identified with six reference individual antigens in PRLIE. The resulting sum pattern of rockets standing on the corresponding lines permits a direct characterization of the antigen content in a number of samples by means of a known line spectrum. The method is simple, reproducible, inexpensive (the polyvalent antiserum was used at 1% in the antibody-containing gel) and allowed quantitative determination.
D000942:Antigens, Bacterial / Q000032:analysis* / Q000145:classification; D006193:Haemophilus influenzae / Q000145:classification / Q000276:immunology*; D007122:Immunoelectrophoresis / Q000379:methods*; D012703:Serotyping / Q000379:methods
D000942:Antigens, Bacterial
Détection et identification antigéniques des Haemophilus influenzae en immunoélectrophorèse quantitative.