[Long-term effects of insulin on the adipocyte conversion of Ob17-line cells].
The Ob17 cell line was established from the epididymal fat pad of ob/ob mice. Chronic exposure to physiological concentrations of insulin led to a net increase in lipogenic enzyme activities (EC50 = 1 to 3 nM). Proinsulin exerted the same effects at higher concentrations (EC50 = 10 to 30 nM). The mitogenic effects of both hormones appeared only at supraphysiological concentrations (EC50 = 300 nM). We conclude this was a net dissociation between the lipogenic and mitogenic effects of insulin during differentiation of the Ob17 cells.
D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D011384:Proinsulin / Q000494:pharmacology; D013997:Time Factors
D007328:Insulin; D008934:Mitogens; D011384:Proinsulin
Effets à long terme de l'insuline sur la conversion adipocytaire des cellules de la lignée Ob17.