Immobilization of E. coli cell as an antigen by radiation polymerization method.
E. coli NIJ cells were immobilized by radiation polymerization of 2-hydroxyethyl methacrylate at low temperatures. The immobilized E. coli cells as an antigen were reacted with peroxidase labeled anti-E. coli in competition with the free E. coli cells. It was found that E. coli cells can be assayed quantitatively with the immobilized E. coli cells in disc form. Microorganisms such as gram negative bacteria had no specific affinity to anti-E. coli. Cross reactivity of the immobilized E. coli cells with the E. coli cells from various strains was examined.
D000907:Antibodies, Bacterial / Q000276:immunology; D000915:Antibody Affinity; D000942:Antigens, Bacterial / Q000276:immunology*; D003037:Cobalt Radioisotopes; D004926:Escherichia coli / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D011108:Polymers / Q000528:radiation effects*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003037:Cobalt Radioisotopes; D011108:Polymers