[Fumarate hydratase activity of various Escherichia coli strains].
The fumarate hydratase activity of intact cells was determined for 36 strains of Escherichia coli, receiver from the All-Union Collection of Microorganisms, to reveal a producer of L-malic acid. A research was made to find optimal media for cultivating microorganisms possessing the fumarate hydratase activity. Spectrophotometric and chromatographic methods were chosen to detect malic acid in the complete reaction mixture, which are available for kinetic study of the malic acid synthesis from potassium fumarate.
D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis / Q000201:enzymology*; D005649:Fumarate Hydratase / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008293:Malates / Q000032:analysis / Q000096:biosynthesis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D003470:Culture Media; D008293:Malates; C030298:malic acid; D005649:Fumarate Hydratase
Fumarat-gidrataznaia aktivnost' razlichnykh shtammov Escherichia coli.