Use of enzyme-linked immunosorbent assay to detect antibodies to staphylococcal protein A in the rabbit.
An ELISA technique is described which employs both solid phase and labelled free SpA. The technique allows the detection of anti-SpA antibodies in the rabbit. It is not suited for the same purpose with human sera, because human immunoglobulins are not saturated by non-specific interaction with solid phase SpA.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity; D013205:Staphylococcal Protein A / Q000276:immunology*
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D013205:Staphylococcal Protein A