Enzyme-linked immunosorbent assay compared with neutralization tests for evaluation of live mumps vaccines.
Mumps-specific antibody levels before and after vaccination with live mumps vaccines were determined by enzyme-linked immunosorbent assay (ELISA) and neutralization tests. A correlation was found between neutralization titers and optical density in ELISA. However, postvaccination sera from some vaccinees who failed to seroconvert by neutralization contained significant levels of mumps-specific antibody detectable by ELISA. In some of these serum specimens, the antibody directed to the F polypeptide of mumps virus was predominant. Most sera positive in ELISA neutralized mumps virus upon the addition of fresh guinea pig serum to the virus-serum mixture.
D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003167:Complement Activation; D004797:Enzyme-Linked Immunosorbent Assay; D015707:HN Protein; D006801:Humans; D007124:Immunoenzyme Techniques; D009108:Mumps Vaccine / Q000276:immunology*; D009500:Neutralization Tests; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D015707:HN Protein; D009108:Mumps Vaccine; D014613:Vaccines, Attenuated; D014764:Viral Proteins