Recognition site of yeast glutathione reductase for 2'-phosphate of NADP+.
Phenyl glyoxal in borate buffer specifically inactivates the NADPH-linked activity of yeast glutathione reductase. While NAD+ is ineffective, NADP+ prevents the enzyme from inactivation, and in the process, protects two arginine residues from glyoxalation. These two arginine residues presumably function as the recognition site for the anionic 2'-phosphate group of NADP+.
D001665:Binding Sites; D005980:Glutathione Reductase / Q000378:metabolism*; D007700:Kinetics; D009249:NADP / Q000378:metabolism* / Q000494:pharmacology; D010658:Phenylglyoxal / Q000494:pharmacology; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D009249:NADP; D005980:Glutathione Reductase; D010658:Phenylglyoxal