Presumptive identification of Staphylococcus saprophyticus from urine specimens by colony appearance and coagulase testing: an evaluation.
Two hundred one urine specimens with pure or nearly pure cultures (greater than 5 X 10(4) CFU/mL) of coagulase-negative staphylococcus were identified in reviewing all significant urine isolates over a ten-month period. The majority of these were from young female adult outpatients with Staphylococcus saprophyticus constituting 88.9% of isolates determined with the API Staph-Ident System. For urine specimens, particularly for outpatients, coagulase-negative, nonhemolytic staphylococcal colonies with yellow pigmentation could be identified presumptively as S. saprophyticus with a sensitivity of 97.8% and a specificity of 98.3%, compared with novobiocin susceptibility testing with a sensitivity of 100% and a specificity of 96.9%.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D003030:Coagulase / Q000032:analysis; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D014556:Urine / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D003030:Coagulase