Immunochemical analysis of glycosylated and nonglycosylated DLA class I antigens.
Dog peripheral blood lymphocytes, when cultured with 35S-methionine in the presence of tunicamycin, synthesize DLA molecules consisting of beta 2-microglobulin and a heavy chain approximately 3000 daltons lower in apparent mol. wt. than observed in control cases. This difference in mol. wt. is consistent with the fact that a single N-linked carbohydrate side chain is present on the heavy chain of DLA class I antigens. There is no evidence of polymorphism in the DLA light chain (beta 2m). Both glycosylated and nonglycosylated forms of the heavy chain, however, show microheterogeneity, which can be related to tissue-type. Analysis by two-dimensional electrophoresis shows that the biochemical heterogeneity in the DLA heavy chain is less than expected from DLA serology, and less than found in HLA class I antigens. The data are consistent with the fact that the products of only a single DLA class I locus are detected.
D000818:Animals; D000937:Antigen-Antibody Reactions; D050260:Carbohydrate Metabolism; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004285:Dogs / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology; D015395:Histocompatibility Antigens Class I; D006650:Histocompatibility Testing; D008297:Male; D011110:Polymorphism, Genetic; D011817:Rabbits; D014415:Tunicamycin; D001613:beta 2-Microglobulin / Q000276:immunology
D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin; C039692:histocompatibility antigen DLA; D014415:Tunicamycin