Microdeposits of amyloid in sclerocalcific heart valves: a histochemical and immunofluorescence study.
Amyloid associated with seven sclerotic and two normal aortic and mitral valves was studied. The sclerotic valve amyloid contained microfibrils with typical random orientation and a fibril width of 9.5-12.5 nm. The amyloid deposits demonstrated permanganate-resistant Congophilia and contained the amino acid tryptophan. Immunofluorescence studies showed P-component in amyloid deposits of 6 of 7 valves, but none of the sclerotic valves contained amyloid fibril proteins of the AL (primary), AA (secondary), AEt (medullary thyroid carcinoma) or ASc1 (senile cardiac) types. Two non-sclerotic valves, removed from a patient with systemic amyloidosis, showed permanganate-sensitive Congophilic amyloid deposits which contained amyloid fibril protein AA.
D000328:Adult; D000368:Aged; D000682:Amyloid / Q000032:analysis; D000686:Amyloidosis / Q000473:pathology*; D001021:Aortic Valve / Q000648:ultrastructure; D002114:Calcinosis / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006349:Heart Valve Diseases / Q000473:pathology*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D008943:Mitral Valve / Q000648:ultrastructure; D012598:Sclerosis; D000683:Serum Amyloid P-Component
D000682:Amyloid; D000683:Serum Amyloid P-Component