In vitro culture of spleen hairy cells.
In vitro culture of hairy cells were successful in 5 patients with hairy cell leukemia: splenic cells were cultured in methyl cellulose plus PHA-leucocyte conditioned stimulation medium for 7 days. Plating efficiency was 0.5% with colony cells having morphological and cytochemical characteristics of hairy cells. A linear correlation between the number of cells plated and the number of colonies produced was found for cell concentrations varying from 5.10(4) to 5.10(5).
D000368:Aged; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013154:Spleen / Q000166:cytology*
D003470:Culture Media