title: Genetic evidence for glucitol-specific enzyme III, an essential phosphocarrier protein of the Salmonella typhimurium glucitol phosphotransferase system.
abstract: Positive selection procedures were developed for the isolation of mutants defective in components of the glucitol-specific catabolic enzyme system in Salmonella typhimurium. gutA (enzyme IIgut-negative), gutB (enzyme IIIgut-negative), and gutC (constitutive for the glucitol operon) mutants were isolated and characterized biochemically and genetically. The gene order was shown to be gutCAB.
mesh_terms: D001692:Biological Transport; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005285:Fermentation; D005798:Genes, Bacterial; D009154:Mutation; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000235:genetics; D013012:Sorbitol / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D013012:Sorbitol; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C034466:phosphoenolpyruvate-sorbitol phosphotransferase