Immunophenotyping of leukaemia: an immunoperoxidase method using air-dried smears.
An immunoperoxidase (IP) method utilizing monoclonal antibodies to demonstrate the presence of the common acute lymphocytic leukaemia antigen (CALLA) on leukaemic cells using air-dried blood and bone marrow smears is described. Two readily available CALLA-specific monoclonal antibodies, BA-3 and J5, and an avidin-biotin immunoperoxidase methodology were utilized. Nineteen cases of acute leukaemia or blast crisis of chronic myelogenous leukaemia were studied. Immunofluorescent studies correlated closely with the results of the immunoperoxidase technique.
D000208:Acute Disease; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm