[Demonstration of hepatitis Be antigen and anti-hepatitis Be with enzyme immunoassay in a microtiter system].
A new microtitre enzyme-immunoassay (Organon Teknika ) to demonstrate HBe-antigen and anti-HBe was compared with a corresponding radioimmunoassay (RIA) of Abbott, on sera of patients with hepatitis-B and reference material. In a serial dilution of HBe-antigen reference preparation from the reference centre for hepatitis B in G ottingen , RIA was able to demonstrate HBe-antigen to a dilution of 1:1024, and the microtitre enzyme-immunoassay (Elisa) to 1:4096 (declaration of reference preparation: 1024 RIA units). In the anti-HBe reference preparation anti-HBe was demonstrated by Elisa to a dilution of 1:4096 and by RIA to 1:512. In tests on sera of patients with hepatitis B, Elisa was superior to RIA in demonstrating both HBe-antigen and anti-HBe. This advantage was particularly clear in the demonstration of anti-HBe. Using the Elisa system, either HBe-antigen or anti-HBe was demonstrated in the fourth week of illness in over 95% of patients. With RIA this result was achieved only in the eighth month after the onset of illness. According to the results obtained with the Elisa system, HBe-antigen is eliminated early and is quickly followed by anti-HBe, while according to results with RIA some time elapsed between the disappearance of HBe-antigen and the occurrence of anti-HBe. Elisa demonstrates high precision, both in a single test and also from test to test, and in this respect, too, it was not inferior to RIA. False-positive or non-reproducible positive reactions were very rare in both tests. These results indicate that Elisa is an alternative to RIA and, because of its higher sensitivity, is superior to RIA.
D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006511:Hepatitis B Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D008832:Microchemistry; D011863:Radioimmunoassay
D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D006735:Horseradish Peroxidase
Nachweis von HBe-Antigen und Anti-HBe mit einem Enzymimmunoassay im Mikrotitersystem.