Comparison of ribosomal proteins of chloroplast from spinach and of E. coli.
A comparison of ribosomal proteins from Escherichia coli and from chloroplasts of Spinach was made using two separate methods: electrophoretic migration and immunochemical cross-reaction between blotted E. coli ribosomal proteins and chloroplast ribosomal subunits antisera. It is shown that L2 from E. coli (E-L2) and L4 from chloroplasts (CS-L4) comigrated and that E-L2 immunologically cross-reacted with the isolated CS-L4 antibody. Co-migration was observed for three additional couples of 50S ribosomal proteins. It is also shown that at least one 30S E. coli ribosomal protein immuno-cross reacted with a 30S chloroplast antiserum and that three couples of 30S ribosomal proteins comigrated.
D000936:Antigen-Antibody Complex; D002736:Chloroplasts / Q000378:metabolism*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics* / Q000302:isolation & purification; D012270:Ribosomes / Q000378:metabolism; D013045:Species Specificity
D000936:Antigen-Antibody Complex; D012269:Ribosomal Proteins