Escherichia coli DNA photolyase is a flavoprotein.
Escherichia coli DNA photolyase (photoreactivating enzyme) was purified to homogeneity from a strain that greatly overproduces the protein. The purified enzyme has absorption peaks at 280 and 380 nm, a fluorescence emission peak at 480 nm and, upon denaturation, releases a chromophore that has the spectroscopic properties of flavin adenine dinucleotide (FAD), indicating that FAD is an intrinsic chromophore of the enzyme.
D004260:DNA Repair / Q000187:drug effects; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005420:Flavoproteins / Q000302:isolation & purification*; D008190:Lyases / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence
D005420:Flavoproteins; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase