title: The origin of VIP-containing nerves in the urinary bladder of rat.
abstract: The innervation of the urinary bladder is known to include a considerable number of nerves containing vasoactive intestinal polypeptide (VIP). The origin of such nerves in the bladder of rat was investigated in this study using the methods of immunocytochemistry and radioimmunoassay combined with surgical sectioning of the hypogastric and/or pelvic nerves to the bladder. Eight days after pelvic nerve sectioning proximal to the main pelvic ganglion, VIP-immunoreactive nerves and VIP content were markedly increased from the level in the sham-operated rat bladder. Sectioning of hypogastric or both nerve pathways led to a less significant increase. It was therefore postulated that the majority of VIP-immunoreactive nerves originate from ganglia located either close to the bladder or within the bladder wall. It is interesting that in these experiments the VIP content of the bladder nerves is inversely related to the changes in motility that would be expected to result from the nerve sections.
mesh_terms: D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011863:Radioimmunoassay; D051381:Rats; D013564:Sympathetic Nervous System / Q000166:cytology / Q000502:physiology; D001743:Urinary Bladder / Q000294:innervation*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
chemical_list: D014660:Vasoactive Intestinal Peptide