title: Cultured human and rat oligodendrocytes and rat Schwann cells do not have immune response gene associated antigen (Ia) on their surface.
abstract: In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibro-blasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.
mesh_terms: D000368:Aged; D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D003337:Corpus Callosum / Q000276:immunology; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007223:Infant; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology*; D051381:Rats; D012583:Schwann Cells / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II