title: Prostacyclin bioassays using inhibition of platelet aggregation and relaxation of rabbit coeliac artery.
abstract: Bioassays of prostacyclin by inhibition of velocity and maximum of both irreversible and biphasic platelet aggregation, and by relaxation of the potassium-contracted isolated superfused rabbit coeliac artery have been undertaken. The use of each parameter is possible within a range of PGI2 concentrations, which have been determined for each of the five parameters studied. The optimal concentrations of PGI2 for a bioassay have also been determined, corresponding to each range.
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D002445:Celiac Artery / Q000187:drug effects; D011464:Epoprostenol / Q000032:analysis* / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010477:Perfusion; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D012044:Regression Analysis
chemical_list: D011464:Epoprostenol