The distance between S1, S21, and the 3' end of 16S RNA in 30S ribosomal subunits. The effect of poly(uridylic acid) and 50S subunits on these distances.
The apparent distances between probes covalently attached to the cysteine thiols of S1 or S21 and the 3' end of 16S RNA in Escherichia coli 30S ribosomal subunits were determined by non-radiative energy transfer to be: S21-16S RNA, 5.1 nm; S21-S1, 6.9 nm; S1-16S RNA, 6.8 nm. Binding of poly(uridylic acid) to 30S subunits causes the apparent distances between S1 and 16S RNA or S21 and 16S RNA to increase by more than 1.2 nm and 0.5 nm, respectively, but has little or no effect on the S1-S21 distance. Binding of 50S subunits causes an apparent increase in the S21-16S RNA and S21-S1 distances by 1.0 nm and 0.8 nm, respectively, but has little or no effect on the S1-16S-RNA distance.
D003374:Coumarins; D004735:Energy Transfer / Q000187:drug effects; D004926:Escherichia coli / Q000648:ultrastructure*; D005452:Fluoresceins; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D011072:Poly U / Q000494:pharmacology*; D012270:Ribosomes / Q000648:ultrastructure*; D013050:Spectrometry, Fluorescence
D003374:Coumarins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011072:Poly U; C027912:fluorescein thiosemicarbazide; C030860:N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide