[Evaluation of the immunoenzymatic method for the study of rubella virus antibodies in relation to the traditional hemagglutination-inhibition test].
The authors compare enzyme immunoassay for rubella virus antibodies (ELISA) with a customary test, hemoagglutino-inhibition test. 392 sera samples were collected from healthy boys and girls; 304 girls had been vaccinated five years before. The authors research sensitivity and specificity of ELISA in comparison with hemoagglutino-inhibition test; ELISA proves to have a high degree of sensitivity, but it is less specific than hemoagglutino-inhibition test. This underlines the possibility to have false positive results from ELISA which appears a very useful test for epidemiological screenings, but less reliable for the diagnosis of recent rubella infections. As ELISA results are usually expressed in Optical Density values, and hemoagglutino-inhibition test results in antibodies dilutions, the authors have performed a graphic analysis; Optical Density values were evaluated in correspondence to the log2 of reciprocal dilutions of hemoagglutino-inhibition test positives. There is evidence of a linear correlation; in this way one can transform ELISA values in their correspondent antibodies dilutions, which are easily understood by general physicians.
D000293:Adolescent; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D012409:Rubella / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral
Valutazione del metodo immunoenzimatico per la ricerca degli anticorpi anti-virus rubeolico in rapporto al tradizionale test di emoagglutinoinibizione.