A new microtechnique for quantitating cell movement in vitro using polystyrene bead monolayers.
In this paper we describe a new and simple technique for studying cell movement in vitro. This method is based upon the migration of endothelial cells on polystyrene bead monolayers. It permits the rapid preparation and screening of multiple 96-well plates for effects on migration, requires fewer samples per test and has the potential for precise quantitation by digital image analysis. The locomotive response of endothelial cells in response to different protein matrices (fibrin, collagen, albumin, gelatin), as used by this technique, is also described. Experiments in progress have shown that this technique can be used to study the effect of different factors (monoclonal antibodies, lymphokines, etc.) on cell migration, and that it may be used with other cell types which exhibit cell migration on substrates in vitro.
D000818:Animals; D001798:Blood Proteins / Q000502:physiology; D002417:Cattle; D002465:Cell Movement; D002478:Cells, Cultured; D003470:Culture Media; D004727:Endothelium / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D005312:Fetal Blood / Q000502:physiology; D007158:Immunologic Techniques; D051379:Mice; D008863:Microspheres; D010587:Phagocytosis; D011137:Polystyrenes
D001798:Blood Proteins; D003470:Culture Media; D011137:Polystyrenes