[Test system for detecting and differentiating the influenza virus by an immunoenzyme method].
Detection and differentiation of small amounts of influenza A and B viruses was done by enzyme-immunoassay based on detection of the complex of internal proteins. It was shown that two kinds of sera to the complex of internal proteins could be used: sera against disrupted viruses grown in a different system (mice or cell culture) and containing almost no CAM component for virus detection in the allantoic fluid of chick embryos, and sera to preparations of viruses grown in chick embryos and disrupted with detergents for elimination of virion surface determinants (wastes of subunit vaccine) for virus detection in nasal secretions of vaccinated subjects. The test-systems containing antibodies to the complex of internal proteins were shown to be as sensitive and specific as those containing antibodies to pure M protein and RNP.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002642:Chick Embryo; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D009981:Influenza B virus / Q000276:immunology / Q000302:isolation & purification*; D007252:Influenza Vaccines / Q000008:administration & dosage; D011817:Rabbits; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D014764:Viral Proteins
Test-sistema dlia indikatsii i differentsiatsii virusa grippa immunofermentnym metodom.