Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues.
A method of extracting proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from plant tissues with high protease activity was described. It resolved protein bands in high-molecular-weight regions of the gel and replaced commonly used procedures which showed severe degradation of proteins, even in the presence of protease inhibitors.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis* / Q000201:enzymology; D011480:Protease Inhibitors / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate; D014238:Trichloroacetic Acid
D010940:Plant Proteins; D011480:Protease Inhibitors; D012967:Sodium Dodecyl Sulfate; D014238:Trichloroacetic Acid; D010447:Peptide Hydrolases