title: Immunohistochemical identification of lymphocyte subsets and accessory cells in human hyperplastic lymph nodes. The functional significance of the compartmentalization of lymphoid tissue.
abstract: Biopsies from 21 lymph nodes with benign hyperplasia were examined by immuno-enzymatic labelling of frozen sections with a panel of monoclonal antibodies. B-cells (B1+, HLA-DR+, C3b receptor+/-) localized in primary follicles, secondary follicles, and areas adjacent to the subcapsular sinus. The B-cells in primary follicles and mantle zones of secondary follicles were indistinguishable ( SmIgD +, SmIgM +, CyIg -, T10-, CALLA-). B-cells adjacent to the subcapsular sinus demonstrated a higher density of SmIgM , and a weaker expression of SmIgD . The germinal centre cells showed a more differentiated phenotype ( SmIgD -, SmIgM +, CyIgM +/-), and also expressed T10 and CALLA. T-cells ( Lyt3 +, Lyt2+, Leu4 +, OKT6-, OKT10 -) localized in paracortial and interfollicular areas, and demonstrated a relative predominance of T-helper/inducer cells ( Leu3 +). T-helper/inducer cells were also identified in secondary follicles. The B-cell areas contained dendritic reticulum cells (R4/23+, C3b-receptor+). Interdigitating reticulum cells (HLA-DR+, OKT6+/-) localized in T-cell regions. The cells in sinuses demonstrated monocyte/macrophage properties (MO2+, Ig+, C3b-receptor+, HLA-DR+/-).
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000145:classification; D002648:Child; D005260:Female; D006801:Humans; D006965:Hyperplasia; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000473:pathology*; D008199:Lymphadenitis / Q000473:pathology; D008214:Lymphocytes / Q000145:classification* / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000145:classification
chemical_list: D000911:Antibodies, Monoclonal