Plasmid containing a DNA ligase gene from Haemophilus influenzae.
A ligase gene from Haemophilus influenzae was cloned into the shuttle vector pDM2 . Although the plasmid did not affect X-ray sensitivity, it caused an increase in UV sensitivity of the wild-type but not excision-defective H. influenzae and a decrease in UV sensitivity of the rec-1 mutant.
D003001:Cloning, Molecular; D011088:DNA Ligases / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D010957:Plasmids; D011118:Polynucleotide Ligases / Q000235:genetics*; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011088:DNA Ligases; D011118:Polynucleotide Ligases