Spatial arrangement of the three alpha helices in the solution conformation of E. coli lac repressor DNA-binding domain.
The relative orientations of the 3 helices in the DNA-binding domain ('headpiece') of lac repressor have been determined using distance constraints obtained from 2-dimensional 1H nuclear Overhauser enhancement spectra. The relative orientations of its helices is similar to that of the central 3 helices in the DNA-binding domain of the lambda repressor of the bacteriophage lambda.
D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000032:analysis*; D011487:Protein Conformation; D012097:Repressor Proteins / Q000378:metabolism*; D012996:Solutions; D013057:Spectrum Analysis; D014157:Transcription Factors / Q000378:metabolism*
D004269:DNA, Bacterial; D012097:Repressor Proteins; D012996:Solutions; D014157:Transcription Factors