Effects of culture filtrates of Blastomyces dermatitidis on neutrophil locomotion.
A potent chemotactic activity for neutrophils is detectable in liquid culture filtrates of Blastomyces dermatitidis. The production of this activity is medium-dependent and culture age-dependent. The highest levels of cytotaxin were produced in filtrates of B. dermatitidis grown in tissue culture medium 199 for three or more weeks. This factor(s) stimulates directed as well as random migration. It functions directly and independently of serum. It is stable at -20 degrees C and 56 degrees C, and has a molecular weight greater than 10000 daltons. These properties define a new microbial chemotactic factor.
D001758:Blastomyces / Q000254:growth & development / Q000378:metabolism*; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D003470:Culture Media; D006801:Humans; D016209:Interleukin-8; D008970:Molecular Weight; D009504:Neutrophils / Q000502:physiology; D013696:Temperature
D002630:Chemotactic Factors; D003470:Culture Media; D016209:Interleukin-8