Structure-activity relationship of the ficin hydrolysis of phenyl hippurates. Comparison with papain, actinidin, and bromelain.
A study of the hydrolysis of 30 substituted-phenyl hippurates by the enzyme ficin has been made. From the results the following quantitative structure--activity relationship (QSAR) has been derived: log 1/Km = 0.79 pi'3 + 0.58 sigma + 0.28 MR4,5 + 3.70. In this expression Km is the Michaelis constant, pi'3 refers to the more hydrophobic of the two meta substituents, and MR4,5 is the molar refractivity of substituents in the 4- and 5-positions of the phenyl ring. This QSAR is compared with those from papain, actinidin, bromelain B, and bromelain D.
D001963:Bromelains / Q000378:metabolism*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000378:metabolism*; D005361:Ficain / Q000378:metabolism*; D006626:Hippurates / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008433:Mathematics; D010206:Papain / Q000378:metabolism*; D013329:Structure-Activity Relationship
D006626:Hippurates; C034677:phenyl hippurate; D001963:Bromelains; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C018745:actinidain; D010206:Papain; D005361:Ficain