Manifestation of K+ transport alterations in cultured tumour cells of mice.
Sarcoma was induced by injection of human adenovirus type 12 into newborn, isogeneic CBA mice and maintained in adult female CBA mice by serial passages. Cells obtained from the tumours were cultivated by 3-4 passages in vitro. Normal fibroblastic cell cultures were gained by the same manner from isogeneic CBA mouse embryos. Characteristics of potassium transport in cultures of malignant cells and of normal fibroblastic cells were analysed. As a chemical tracer of K+ movements, 86Rb+ was applied. No significant difference could be detected either in the potassium concentration, or in the 86Rb+ uptake of the two types of cultured cells. However, when the cells were exposed to ouabain, the malignant cells showed a significantly reduced response, thus, the malignant cells accomplished a much less decrease in either cellular potassium concentration or in that of 86Rb+ uptake rate than the normal cells. These findings well fit the hypothesis advanced by several authors that malignant cells have a reduced density of ouabain receptor on the membrane.
D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D008858:Microscopy, Phase-Contrast; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011868:Radioisotopes; D011955:Receptors, Drug / Q000187:drug effects; D012413:Rubidium; D012513:Sarcoma, Experimental / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase
D011868:Radioisotopes; D011955:Receptors, Drug; C028996:cardiac glycoside receptors; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D011188:Potassium