title: Plasma membrane lipid order of leukemic and normal immature avian erythroid cells.
abstract: Mammalian erythroblasts and their leukemic counterparts contain characteristic disordered regions of plasma membrane identified as putative membrane protein collection sites. In order to determine whether erythroid cells which do not enucleate contain homologous membrane domains, immature avian erythroid precursor cells and avian erythroleukemic cells were examined using merocyanine 540 (MC540), a fluorescent dye whose binding is sensitive to the packing of membrane lipids. Results were found to contrast with previous studies of the murine equivalents of these cells. In birds, normal erythroid precursors, including basophilic erythroblasts from the bone marrow and spleen of anemic animals, contained no detectable (less than 0.1%) cells which were stained by the dye. But cells from chicks infected with avian erythroblastosis virus (AEV) did stain. Considering the pattern of staining observed on AEV-erythroblasts relative to other leukemic and normal phenotypes, however, we conclude that neither normal nor leukemic avian erythroid cells contain a functional equivalent to the membrane protein collection sites found on their mammalian counterparts.
mesh_terms: D001355:Alpharetrovirus; D000818:Animals; D002645:Chickens; D004910:Erythrocyte Membrane / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007942:Leukemia, Experimental / Q000097:blood*; D008563:Membrane Lipids / Q000097:blood*; D012016:Reference Values
chemical_list: D008563:Membrane Lipids