Purification and reversible subunit dissociation of overproduced Escherichia coli phenylalanyl-tRNA synthetase.
Phenylalanyl-tRNA synthetase (EC 6.1.1.20) has been purified to homogeneity from a 100-fold overproducing Escherichia coli strain carrying a hybrid pBR322 plasmid containing the pheS-pheT locus. The purified enzyme is identical to the phenylalanyl-tRNA synthetase isolated form an haploid strain. The enzyme was found to dissociate in the presence of 0.5 M NaSCN and the alpha- and beta-subunits composing the native alpha 2 beta 2 enzyme were separated by gel filtration. Neither isolated subunit showed significant catalytic activity. A complex indistinguishable from the native enzyme with full catalytic activity is recovered upon mixing the subunits. The N- and C-terminal sequences and the amino acid composition of each subunit were determined. They are compared to the available data concerning the primary structure of the subunits, as deduced from nucleotide sequencing of the pheS-pheT operon.
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics / Q000302:isolation & purification*
D046911:Macromolecular Substances; D010446:Peptide Fragments; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase