[Comparison of the physical properties of ribosomal proteins from Escherichia coli 50S subparticles isolated by different methods].
Physical properties of ribosomal proteins obtained with or without denaturing agents were compared. CD measurements and PMR studies have shown that proteins L2, L19, L24 and L30 isolated under denaturing conditions have the same properties as those prepared avoiding denaturing agents. CD and PMR data of L1, L6, L11, L23, L25 and L29 obtained by us under denaturing conditions practically coincide with the data for these proteins obtained in "mild" conditions and published in the literature. These findings indicate that the differences of physical properties reported in the literature can be due to different procedures of protein renaturation rather than to the methods of their isolation.
D002942:Circular Dichroism; D004926:Escherichia coli / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D011489:Protein Denaturation; D012269:Ribosomal Proteins / Q000302:isolation & purification*
D012269:Ribosomal Proteins
Sravnenie fizicheskikh svoĭstv ribosomnykh belkov 50S subchastitsy Escherichia coli, poluchennykh razlichnymi metodami.