[Use of monoclonal antibody 225.28S in the study of nevus cells in culture].
The aim of this work is to identify and purify cultured nevus cells. We used for our study monoclonal antibody 225.28S. This antibody reacts with a surface antigen which is expressed by nevus cells and melanoma cells, but it does not react with normal melanocytes. We studied 10 dermic nevi and we observed that antigenic determinant survives in cultured nevus cells. These results allowed us to employ the method of panning for purify cultured nevus cells.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D009506:Nevus / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface
Impiego dell'anticorpo monoclonale 225.28S nello studio delle cellule neviche in coltura.