title: UV-induced imbalance of the deoxyribonucleoside triphosphate pool in E. coli.
abstract: The effect of UV irradiation on the intracellular DNA precursor pool in E. coli was investigated. UV irradiation of E. coli, followed by post-incubation for 1-1.5 h, altered the relative sizes of the deoxyribonucleoside triphosphate (dNTP) pool. The total amount of dNTPs increased: both dATP and dTTP increased several-fold, dCTP about twofold, while dGTP remained almost unchanged. In recA- and umuC- strains, which are defective in UV-induced mutagenesis, the pattern of nucleotide pool alterations was similar to that of wild-type strains.
mesh_terms: D004261:DNA Replication / Q000528:radiation effects; D003854:Deoxyribonucleotides / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000254:growth & development / Q000528:radiation effects*; D009154:Mutation; D014466:Ultraviolet Rays
chemical_list: D003854:Deoxyribonucleotides