title: Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium.
abstract: The metabolism of trehalose in wild type cells of Escherichia coli and Salmonella typhimurium has been investigated. Intact cells of Escherichia coli (grown on trehalose) accumulated [14C]-trehalose as [14C]-trehalose 6-phosphate. Toluene-treated cells catalyzed the synthesis of the [14C]-sugar phosphate from [14C]-trehalose and phosphoenolpyruvate; ATP did not serve as phosphoryl donor. Trehalose 6-phosphate could subsequently be hydrolyzed by trehalose 6-phosphate hydrolase, an enzyme which catalyzes the hydrolysis of the disaccharide phosphate into glucose and glucose 6-phosphate. Both Escherichia coli and Salmonella typhimurium induced this enzyme when they grew on trehalose. These findings suggest that trehalose is transported in these bacteria by an inducible phosphoenolpyruvate:trehalose phosphotransferase system. The presence of a constitutive trehalase was also detected.
mesh_terms: D001692:Biological Transport; D004186:Disaccharidases / Q000378:metabolism; D004187:Disaccharides / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*; D014198:Trehalase / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D014199:Trehalose; D004186:Disaccharidases; D014198:Trehalase; C040631:alpha, alpha-phosphotrehalase; D005947:Glucose; D005990:Glycerol