title: Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes.
abstract: Nuclei of amphibian oocytes contain large amounts of actin, mostly in unpolymerized or short-polymer form. When antibodies to actin or actin-binding proteins (fragmin and the actin modulator from mammalian smooth muscle) are injected into nuclei of living oocytes of Pleurodeles waltlii, transcription of the lampbrush chromosomes, but not of the rRNA genes, is inhibited. When transcription is repressed by drugs or RNA is digested by microinjection of RNAase into oocyte nuclei, an extensive meshwork of actin filament bundles is seen in association with the isolated lampbrush chromosomes. These observations indicate a close relationship between the state of nuclear actin and transcriptional activity and suggest that nuclear actin may be involved in transcriptional events concerning protein-coding genes.
mesh_terms: D000199:Actins / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins / Q000008:administration & dosage; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002875:Chromosomes / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D018260:Gelsolin; D008840:Microfilament Proteins; D008845:Microinjections; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism; D010999:Pleurodeles; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; C016953:brevin