Reduced levels of fibronectin in ataxia-telangiectasia lymphoblastoid cells.
In this study Epstein-Barr virus-transformed lymphoblastoid cells were shown to contain fibronectin. A marked difference in the number of fibronectin-positive cells was observed in control and ataxia-telangiectasia lymphoblastoid cells. Three control cell lines varied between 60% and 80% in the number of cells containing fibronectin. Only a small percentage of ataxia-telangiectasia cells (1-5%) had fibronectin present. Control and ataxia-telangiectasia fibroblasts had similar amounts and distributions of fibronectin. An asymmetric distribution of fibronectin was evident in many control and ataxia-telangiectasia lymphoblastoid cells. The increased frequency of fibronectin-containing cells in the control populations cannot be accounted for by different growth rates or variation in the various phases of the cell cycle in the two cell types.
D000911:Antibodies, Monoclonal / Q000032:analysis; D001260:Ataxia Telangiectasia / Q000276:immunology / Q000473:pathology*; D002453:Cell Cycle; D002460:Cell Line; D005347:Fibroblasts / Q000032:analysis / Q000473:pathology; D005353:Fibronectins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000473:pathology
D000911:Antibodies, Monoclonal; D005353:Fibronectins