Fermentation, isolation, characterization and structure of nitrosofungin.
The new antifungal agent nitrosofungin was isolated in high yields from a mixed culture of two organisms consisting of a bacterium of the genus Alcaligenes (UC 9152) and Streptomyces plicatus UC 8272. The bacterium produces the agent, the streptomycete enhances the production by providing a precursor or an inducer. Nitrosofungin in high concentrations inhibits a broad variety of pathogenic fungi in vitro. The agent is relatively non-toxic in small laboratory animals and high blood levels are obtained after either oral or systemic administration. Nitrosofungin is only the second N-nitrosohydroxylamine isolated from microbial sources to date. It has been identified as 2-N-nitrosohydroxylamino-1-propanol, an acidic and highly water-soluble compound.
D000421:Alcaligenes / Q000254:growth & development*; D000935:Antifungal Agents / Q000302:isolation & purification*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004355:Drug Stability; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D009602:Nitrosamines / Q000302:isolation & purification / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013053:Spectrophotometry; D013302:Streptomyces / Q000254:growth & development*
D000935:Antifungal Agents; D009602:Nitrosamines; C039121:nitrosofungin