Immunologic observations in sera of a patient with allergic bronchopulmonary aspergillosis by means of the enzyme-linked immunosorbent assay.
Titers of IgG, IgA, and IgM antibodies against Aspergillus fumigatus were measured by ELISA in sera of a patient with allergic bronchopulmonary aspergillosis during an acute stage, remission, and exacerbation of the disease. Total IgE, specific IgE against A. fumigatus, and number of precipitation lines were also measured. ELISA IgG-, IgA-, and IgM-antibody titers were expressed in percentages of the titer of a strongly positive standard serum by use of the patient's own serum as a standard. The fall in antibody concentration (in percent) and elevations during exacerbation appeared to be similar for these antibody classes and are related with the number of precipitates measured by double immunodiffusion. Furthermore, the changes in antibody titers were also similar to the elevations and decreases in both total IgE and specific-IgE antibodies (also expressed in percentages of a chosen standard serum). The ELISA IgG-antibody titers were higher compared with the ELISA IgA or IgM titers and demonstrated a continuous decline during a period of 2 yr after the acute phase of ABPA. Therefore, IgG-antibody titers are preferred as an indicator of disease activity in follow-up studies over longer time periods and are at least as sensitive as the measurement of concentrations of IgE antibodies.
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000946:Antigens, Fungal / Q000276:immunology; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D012075:Remission, Spontaneous
D000888:Antibodies, Anti-Idiotypic; D000946:Antigens, Fungal; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M