[Choice of the initial enzyme correlation in immobilizing polyenzyme systems].
An optimal, in respect to productivity (activity X stability), enzyme ratio for immobilization of multienzyme systems was calculated by using the kinetic parameters (KM and Vmax), data on the stability and yield of each enzyme during immobilization. The experimental data, obtained during combined immobilization of invertase, mutarotase and glucose oxidase, illustrate the theoretical propositions.
D000818:Animals; D002238:Carbohydrate Epimerases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008433:Mathematics; D010407:Penicillium / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity; D013552:Swine; D043324:beta-Fructofuranosidase
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase
Vybor iskhodnogo sootnosheniia fermentov pri immobilizatsii polifermentnykh sistem.