An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity.
A selective system toxic towards mammalian cells expressing the liver-specific isozyme of alcohol dehydrogenase (L-ADH) has been developed. A number of alpha-unsaturated primary and secondary alcohols were assayed for their ability to serve as substrates for rat liver ADH and were screened for cytotoxicity towards L-ADH+ and L-ADH- cells. 1-Propen-3-ol and 1-penten-3-ol were identified as agents showing selective cytotoxicity. Reconstruction experiments demonstrated that 1-propen-3-ol at a concentration of 15 microM could be used to recover L-ADH- clones from mixed populations of L-ADH+ and L-ADH cells. Cells expressing the non-allelic S-ADH isozyme were not killed under these conditions. The selective system defined in this report is thus isozyme-specific.
D000433:1-Propanol / Q000494:pharmacology; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000172:deficiency* / Q000235:genetics; D000438:Alcohols / Q000494:pharmacology; D000483:Alleles; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells; D003470:Culture Media; D007527:Isoenzymes / Q000172:deficiency* / Q000235:genetics; D007739:L Cells / Q000166:cytology / Q000201:enzymology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology; D051379:Mice; D000439:Pentanols; D010641:Phenotype; D020005:Propanols; D051381:Rats; D013379:Substrate Specificity
D000438:Alcohols; D003470:Culture Media; D007527:Isoenzymes; D000439:Pentanols; D020005:Propanols; C006463:allyl alcohol; C010279:1-pentyn-3-ol; D000433:1-Propanol; C068720:1-penten-3-ol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase