Interaction of antimalarial drugs with hemin.
Hemin (ferriprotoporphyrin IX) is shown to form complexes with the chloroquine class of antimalarial drugs. The Soret band of hemin becomes optically active upon the addition of chiral drugs. Results on the hemin-induced quenching of the fluorescence of chloroquine are consistent with the formation of a 2:1 hemin:drug complex with a formation constant of 1.4 x 10(7) at 298 K. Also a direct comparison of the drug-treated and drug-free parasites themselves, by the noninvasive photoacoustic spectroscopic method, reveals an in vivo interaction between endogenous hemin and the added drug.
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D002738:Chloroquine / Q000494:pharmacology*; D002942:Circular Dichroism; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000378:metabolism*; D010962:Plasmodium berghei / Q000187:drug effects*; D011802:Quinidine / Q000494:pharmacology*; D011803:Quinine / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
D000962:Antimalarials; D006418:Heme; D006427:Hemin; D002738:Chloroquine; D011803:Quinine; D011802:Quinidine