Natural killer cells in renal allograft rejection.
Monoclonal antibody Anti Leu 7 has been used to enumerate peripheral blood NK cells in 20 renal allograft recipients. Blood was taken preoperatively and three times per week for the first month after transplantation; separated mononuclear cells were stained with Anti Leu 7 then analysed on a fluorescence activated cell sorter. Results were related to acute rejection episodes diagnosed clinically and confirmed by Trucut biopsy of grafts. A reduction in the number of Leu 7 positive cells occurred postoperatively. Nineteen out of 20 patients showed a rise in number of circulating NK cells during acute rejection episodes.
D000911:Antibodies, Monoclonal; D002469:Cell Separation; D003404:Creatinine / Q000097:blood; D005434:Flow Cytometry; D006084:Graft Rejection; D006801:Humans; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000166:cytology / Q000502:physiology*; D007958:Leukocyte Count; D011184:Postoperative Period; D013997:Time Factors
D000911:Antibodies, Monoclonal; D003404:Creatinine