title: Predominant attached state of myosin cross-bridges during contraction and relaxation at low ionic strength.
abstract: The chemical states of a cross-bridge--nucleotide complex were studied using a fluorescent ATP analogue, 1-N6-etheno-2-aza-ATP(epsilon-2-aza-ATP). The fluorescence of epsilon-2-aza-ATP at specific emission wavelengths was enhanced by 12.5 times upon binding to myosin in a relaxed muscle and the fluorescence from the resultant myosin(M)-epsilon-2-aza-ADP-Pi intermediate was 2.5 times greater than that from a M-epsilon-2-aza-ADP complex. Similar enhancements of the fluorescence of epsilon-2-aza-ATP and epsilon-2-aza-ADP were observed upon binding to heavy meromyosin in solution. Binding of F-actin did not change the fluorescence of epsilon-2-aza-ATP or epsilon-2-aza-ADP bound to heavy meromyosin. When a muscle went from a relaxed state to a state of isometric contraction or contraction with shortening, the fluorescence intensity decreased only slightly or not at all, i.e. the fluorescence of nucleotides bound to most of the myosin heads during contraction is the same as that of the M-epsilon-2-aza-ADP-Pi intermediate. These results suggest that an actomyosin(AM)-epsilon-2-aza-ADP-Pi intermediate is the predominant attached state during contraction. When the ionic strength of the relaxing solution was decreased, cross-bridges formed at 6 degrees C without tension generation. At 20 degrees C, a large tension was produced although the shortening velocity was negligibly small or zero. The fluorescence intensity decreased by 15% at 20 degrees C but only a small decrease of 3% was observed at 6 degrees C, suggesting that the predominant complexes in the attached state were AM-epsilon-2-aza-ATP and/or AM-2-aza-ADP-Pi at 6 degrees C and AM-epsilon-2-aza-ADP at 20 degrees C. Thus, the identification of the actomyosin-nucleotide complexes existing before and after the force-generating step lent further support to the conclusion that the sliding force is generated by conformational changes in actomyosin when the (epsilon-2-aza-)ADP-Pi complex is bound to it.
mesh_terms: D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D004985:Ethenoadenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques; D007537:Isometric Contraction; D046911:Macromolecular Substances; D009119:Muscle Contraction; D009126:Muscle Relaxation; D009132:Muscles / Q000378:metabolism; D015879:Myosin Subfragments / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism; D009994:Osmolar Concentration; D011487:Protein Conformation; D011817:Rabbits; D013050:Spectrometry, Fluorescence
chemical_list: D004985:Ethenoadenosine Triphosphate; D046911:Macromolecular Substances; D015879:Myosin Subfragments; D009711:Nucleotides; C042937:aza-epsilon-ADP; C012758:aza-epsilon-ATP; D000244:Adenosine Diphosphate; D009218:Myosins