Autoantibodies against Leydig cells in patients after spermatic cord torsion.
This study is aimed at searching for the presence of circulating antibodies against frozen sections of human testis, ovary and trophoblast in patients that had spermatic cord torsion. Sixty-eight sera samples were studied. Nine patients (13.2%) were positive for organ specific anti-testis autoantibodies. Six patients were positive for antibodies against Leydig cells: five were positive only with the indirect immunofluorescence technique of complement fixing (ITT/CF), the sixth patient was positive only with the indirect immunofluorescence technique (ITT). The other three patients were positive for antibodies against germ line cells: two patients were positive with both techniques, the third was positive only with indirect immunofluorescence technique. Eight of these patients were negative for antibodies against adrenal cortex while only one case was positive with indirect immunofluorescence technique both on adrenal cortex and Leydig cells. Human lyophilized testis absorbed the reactive antibodies against Leydig cells and germ line cells, while adrenal cortex and lyophilized testosterone were ineffective. This study shows the identification of a specific antibody against Leydig cells and germ line cells in patients after spermatic cord torsion.
D000293:Adolescent; D000302:Adrenal Cortex / Q000276:immunology; D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D002648:Child; D003168:Complement Fixation Tests; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007985:Leydig Cells / Q000276:immunology*; D008297:Male; D012708:Sertoli Cells / Q000276:immunology; D013086:Spermatic Cord Torsion / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology; D013737:Testis / Q000276:immunology
D001323:Autoantibodies