A microtiter assay for human monocyte activation by lymphokines.
This report describes a microtiter assay of human lymphokine activity based on lymphokine-induced increases in monocyte adherence to plastic surfaces. Supernatants were generated by stimulation of peripheral blood mononuclear cells with streptokinase/streptodornase or purified protein derivative. Monocyte adherence was measured by a 51chromium radioassay. The increased adherence was shown to be due to a soluble factor that was produced by antigen-stimulated mononuclear cells from donors who were responsive to the antigen but not by mononuclear cells from donors who were unresponsive. The factor was not dialyzable. The assay requires only 20 ml of peripheral blood and is easily automated and quantifiable, making it suitable for clinical laboratory use.
D002448:Cell Adhesion; D002452:Cell Count / Q000379:methods; D002860:Chromium Radioisotopes; D003956:Dialysis; D006801:Humans; D007158:Immunologic Techniques / Q000295:instrumentation*; D008222:Lymphokines / Q000096:biosynthesis / Q000502:physiology*; D008262:Macrophage Activation; D009000:Monocytes / Q000276:immunology* / Q000502:physiology
D002860:Chromium Radioisotopes; D008222:Lymphokines