title: Quantification of bands produced by isoelectric focusing using immunoperoxidase.
abstract: An improved, rapid, and sensitive (to autoradiographic levels) method for the quantification of IgG bands from cerebrospinal fluid is presented. The principle of an internal standard is employed using the discrete bands of a paraprotein to correct for any run to run variability in the enzyme detection system which is linked to the immunoassay of immobilised antigen.
mesh_terms: D004587:Electrophoresis, Agar Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing / Q000379:methods; D010266:Paraproteins / Q000134:cerebrospinal fluid*
chemical_list: D007074:Immunoglobulin G; D010266:Paraproteins