Spermidine inhibits degradation of yeast chromatin.
A procedure has been developed for the isolation of intact yeast chromatin from yeast nuclei. Autodigestion of chromatin observed during nuclear preparation was inhibited by the addition of 5 mM spermidine. The procedure is useful for the analysis of proteins associated with yeast chromatin.
D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000032:analysis*; D002843:Chromatin / Q000302:isolation & purification* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013095:Spermidine / Q000494:pharmacology*
D002843:Chromatin; D013095:Spermidine