title: Prevalence of antibodies to porcine adenovirus in swine by indirect fluorescent antibody test.
abstract: An indirect fluorescent antibody test was developed for routine identification of a porcine adenovirus and its specific antibody. Two specific-pathogen-free young pigs were inoculated with the viral antigen prepared in continuous porcine kidney cell cultures, and their sera were used as an antibody reagent to standardize the test. Sera of adult pigs with respiratory problems, obtained from pig farms in Quebec, were tested for antibodies to this virus; 83 of 540 sera tested (15.2%) were found to be positive.
mesh_terms: D000256:Adenoviridae / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D007668:Kidney; D008854:Microscopy, Electron; D011792:Quebec; D012140:Respiratory Tract Diseases / Q000276:immunology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral