Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.
We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.
D002503:Centromere; D002874:Chromosome Mapping; D002875:Chromosomes; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal; D008540:Meiosis; D008938:Mitosis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal