Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin.
A sandwich enzyme-linked immunosorbent assay was developed for measuring Staphylococcus aureus alpha-toxin. This assay was 500 to 1,000 times more sensitive than the commonly used hemolytic titration assay and was less variable. The binding of alpha-toxin to the adsorbed antibody was most effective after an overnight incubation at 27 degrees C. The toxin was detectable even at a log2 17 dilution of an S. aureus culture supernatant.
D001427:Bacterial Toxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006460:Hemolysin Proteins; D006462:Hemolytic Plaque Technique; D007124:Immunoenzyme Techniques; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000472:pathogenicity
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D013205:Staphylococcal Protein A; C021091:staphylococcal alpha-toxin