A method to select cell populations with enhanced chemotactic activity.
Virus-transformed and tumor cells often show a higher chemotactic activity in comparison to normal cells. Here we show that the blind-well chamber assay is an easy and rapid procedure to select cell populations with enhanced chemotactic activity. Collagen-type-specific antibodies have been applied to analyze the efficiency of cell sorting by the chemotaxis assay.
D002469:Cell Separation / Q000379:methods*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002633:Chemotaxis; D003094:Collagen / Q000276:immunology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005354:Fibrosarcoma / Q000473:pathology / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D006801:Humans
D003094:Collagen