title: The relationship between intracellular pH, the pH gradient and potassium transport in Escherichia coli.
abstract: The capacity of E. coli cells to regulate intracellular pH (pHi) during net potassium uptake has been investigated. The data show: (a) that cells sense their intracellular pH; (b) that the pH gradient (delta pH) exerts a feedback regulation on pHi; (c) that a mechanism of regulation of pHi exists which may be independent of Na+ [Zilberstein, Agmon, Schuldiner & Padan (1982) J. Biol. Chem. 257, 3687-3691]; and (d) that cells have a limited capacity to raise their intracellular pH in the absence of net K+ transport.
mesh_terms: D001692:Biological Transport; D004926:Escherichia coli / Q000378:metabolism*; D005246:Feedback; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005947:Glucose; D005990:Glycerol; D011188:Potassium