title: Characterization of the C1q receptor on a human macrophage cell line, U937.
abstract: The binding of C1q to the human macrophage cell line U937 has been studied. Fluorescence microscopy with fluorescein-conjugated F(ab')2 anti-C1q antibody showed that 100% of the cell population is able to bind exogenous C1q. Monomeric C1q binding to U937 cells is very weak at normal ionic strength (I0.15) and was therefore investigated at I0.07, conditions which stabilize the binding. However, aggregation of C1q on dextran sulphate or a lipid A-rich lipopolysaccharide allowed a firm, binding at I0.15. Quantitative binding studies with monomeric 125I-C1q showed a concentration-dependent, saturable, specific and reversible binding involving specific membrane receptors. Scatchard plots of C1q binding indicated [1.6 +/- 0.7 (1 S.D.)] X 10(6) sites per cell with an equilibrium constant of (2.9 +/- 1.8) X 10(7) M-1 at I0.07. The location of the molecule region mediating C1q binding was established with collagen-like fragments prepared by partial pepsin digestion, confirming earlier results obtained by inhibition studies.
mesh_terms: D001665:Binding Sites; D002352:Carrier Proteins; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D018960:Hyaluronan Receptors; D007700:Kinetics; D008264:Macrophages / Q000276:immunology*; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D009994:Osmolar Concentration; D010446:Peptide Fragments / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology; D011951:Receptors, Complement / Q000187:drug effects / Q000378:metabolism*
chemical_list: C087299:C1QBP protein, human; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D010446:Peptide Fragments; D011951:Receptors, Complement; C032402:complement 1q receptor; D003094:Collagen; D010447:Peptide Hydrolases