Electron microscopy of tRNA crystals. II. 4 A resolution diffraction pattern and substantial stability to radiation damage.
Electron microscopy was applied to thin crystals of yeast tRNAPhe. The crystals embedded in glucose yield Bragg reflections with a spacing smaller than 4 A. The measurement of radiation damage rate demonstrates that they are 4 to 14 times less susceptible to electron exposures than protein crystals embedded in glucose.
D001436:Bacteriorhodopsins / Q000528:radiation effects; D002374:Catalase / Q000528:radiation effects; D003460:Crystallization; D004583:Electrons; D008854:Microscopy, Electron; D012346:RNA, Transfer, Amino Acyl / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000032:analysis
D012346:RNA, Transfer, Amino Acyl; D001436:Bacteriorhodopsins; D002374:Catalase