Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.
Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D000918:Antibody Specificity; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D046911:Macromolecular Substances
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D004274:DNA, Recombinant; D046911:Macromolecular Substances; D004247:DNA