ATP-gamma-S (adenosine 5'-0(3-thiotriphosphate)) blocks progesterone-induced maturation of the Xenopus oocyte.
ATP-gamma-S microinjection into Xenopus oocyte prevents progesterone induced maturation. Inhibition is time and dose dependent; 50% inhibition occurs when 50 nl of 0.5 mM ATP-gamma-S solution are microinjected/oocyte 1 hr prior to the hormonal trigger. ATP-gamma-S inhibited oocytes can be induced to mature (100%) following microinjection of extracts containing maturation promoting factor (MPF). Our results suggest that the maturation protein(s) has been stabilized in ovo by ATP-gamma-S microinjection, in its phosphorylated inhibitory form.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D016200:Maturation-Promoting Factor; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects*; D010750:Phosphoproteins / Q000502:physiology; D011374:Progesterone / Q000037:antagonists & inhibitors*; D013873:Thionucleotides / Q000494:pharmacology*; D014982:Xenopus laevis
D006133:Growth Substances; D010750:Phosphoproteins; D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D011374:Progesterone; D000255:Adenosine Triphosphate; D016200:Maturation-Promoting Factor