Gold-IgG complexes improve the detection and identification of viruses in leaf dip preparations.
An immunoelectron microscopic method is described for rapid detection and identification of plant viruses in leaf-dip preparations by specific labelling with gold-IgG complexes. The process takes only 10 min and markedly improves the identification of viruses, particularly spherical ones including luteoviruses in leaf dips over simple antibody decoration.
D006046:Gold; D007074:Immunoglobulin G / Q000276:immunology*; D008828:Microbiological Techniques; D008854:Microscopy, Electron; D010942:Plant Viruses / Q000302:isolation & purification*
D007074:Immunoglobulin G; D006046:Gold