Lung-stage expression of a major schistosome surface antigen.
The topographical expression of a glycoprotein of 180,000 molecular weight on the surface of lung-stage Schistosoma mansoni schistosomula was determined by immunofluorescence microscopy using a monoclonal antibody. Postfixation treatment with graded ethanols enhanced specific immunofluorescent staining of adult worms, and was required for detection of the antigen on the surfaces of lung-stage schistosomula. The epitope recognized by the monoclonal antibody was also present on the surfaces of adult Schistosoma haematobium, but not on those of Schistosoma japonicum.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D006224:Cricetinae; D005260:Female; D005455:Fluorescent Antibody Technique; D008168:Lung / Q000469:parasitology*; D008642:Mesenteric Veins / Q000469:parasitology*; D008647:Mesocricetus; D051379:Mice; D012548:Schistosoma haematobium / Q000276:immunology / Q000469:parasitology*; D012549:Schistosoma japonicum / Q000276:immunology / Q000469:parasitology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology / Q000469:parasitology*
D000954:Antigens, Surface