Comparison of sensitivities of ELISA and radioimmunoassay for detection of class-specific antibody in mouse serum.
The limits of detection of IgG1 antibody in a standard mouse antiserum by a single antiglobulin enzyme-linked immunosorbent assay (ELISA) and 2 amplified systems, a double antiglobulin ELISA and a double antiglobulin radioimmunoassay (RIA), were compared in microtitration plates with the same antigen preparation and antisera. Compared with the single antiglobulin ELISA, both amplified assays demonstrated a 64-fold increase in sensitivity for the detection of antibody at high dilutions of standard antiserum. It is concluded that the amplified ELISA offers a safer assay than the amplified RIA and of equal sensitivity for comparable consumption of antisera.
D000469:Alkaline Phosphatase; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards; D012709:Serum Albumin / Q000276:immunology; D012756:Sheep
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D012709:Serum Albumin; D000469:Alkaline Phosphatase