Hemin-induced conversion of pyruvate kinase isozymes in K562 cells.
The effects of hemin on the conversion of pyruvate kinase (PK) isozymes from the M2-type to the L-type in K562 cells were investigated. Immunofluorescence, ion exchange chromatography, and electrophoretic studies showed that the untreated K562 cells contained only the M2-type PK, while eight to 20 days after induction with hemin, concomitant with hemoglobin F synthesis, L-type PK levels increased while M2-type PK levels decreased. Electrophoretic study revealed three hybrid isozymes of the L-type and M2-type PK. We conclude that the conversion of PK isozymes from the M2-type to the L-type in erythroid precursor cells occurs in the early stage of maturation.
D002460:Cell Line; D002852:Chromatography, Ion Exchange; D002869:Chromosome Aberrations / Q000097:blood / Q000235:genetics; D025063:Chromosome Disorders; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D007951:Leukemia, Myeloid / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D011770:Pyruvate Kinase / Q000097:blood* / Q000302:isolation & purification
D007527:Isoenzymes; D006418:Heme; D006427:Hemin; D011770:Pyruvate Kinase