Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: a review of methods and applications.
Proteins and other macromolecules can be injected into cultured cells by several different methods. Here we review the strengths and limitations of two of these methods, red-cell-mediated microinjection and osmotic lysis of pinosomes, and indicate how they may be successfully applied to the study of cultured cells.
D000818:Animals; D002478:Cells, Cultured; D004705:Endocytosis; D004910:Erythrocyte Membrane; D008561:Membrane Fusion / Q000187:drug effects; D008845:Microinjections / Q000379:methods*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000494:pharmacology; D011506:Proteins / Q000008:administration & dosage
D011506:Proteins; D011092:Polyethylene Glycols