title: Inhibition of antibody responses to phosphocholine by C-reactive protein.
abstract: C-reactive protein (CRP) is an acute phase serum protein in man that binds to the cell wall C-polysaccharide (PnC) of Streptococcus pneumoniae via phosphocholine (PC) determinants. We have previously shown that in mice CRP increases splenic clearance of PnC-coated autologous erythrocytes and S. pneumoniae, and increases survival after pneumococcal infection. Because CRP alters clearance of particulate PnC antigens, we tested its effect on immunization with pneumococci. Pretreatment of mice with 50 to 200 micrograms CRP 30 min before immunization with serotype 3 S. pneumoniae resulted in dose-dependent inhibition of the antibody response to PC. Both serum hemagglutinin and splenic PFC against PC were decreased in CRP-treated mice tested from 1 to 10 days after injection of antigen. CRP treatment had no effect on the antibody response to the serotype 3 capsular polysaccharide, another T-independent antigen. To determine whether CRP inhibition was related to altered processing of particulate antigen, mice were immunized with horse red blood cells (HRBC) conjugated with PC or PnC and the PFC responses to PC and HRBC were determined. CRP treatment resulted in specific inhibition of the PFC response to PC in both cases without affecting the response to HRBC. These results indicate that inhibition of the antibody response by CRP is not the result of altered antigen localization and processing, and that CRP may prevent immunization by masking determinants on bacterial or other surfaces.
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects*; D001428:Bacterial Vaccines / Q000008:administration & dosage; D001667:Binding, Competitive; D002097:C-Reactive Protein / Q000008:administration & dosage*; D002794:Choline / Q000031:analogs & derivatives*; D004546:Elapid Venoms / Q000008:administration & dosage; D005260:Female; D006462:Hemolytic Plaque Technique; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology*; D022242:Pneumococcal Vaccines; D013997:Time Factors
chemical_list: D001428:Bacterial Vaccines; D004546:Elapid Venoms; D022242:Pneumococcal Vaccines; C016310:cobra venom factor; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D002794:Choline