[Independence from the substrate of multiplication in nonclonal and clonal tumor cell populations].
Experiments were made with sarcoma PS-103 cells cultured in vitro. The sarcoma was induced by implantation of a plastic plate into CBA mice. Clonal analysis of the cell culture demonstrated that 1) all 3 clones isolated from substrate (SB) grew in 1.2% methyl cellulose (MC) at the same efficiency as parental cells; 2) all 5 clones isolated from MC formed in a semi-solid medium 10-100-fold more colonies than PS-103. During the subcloning of one of PS-103 clones in solid substrate and in MC, it turned out that the majority of MC and SB subclones had the plating efficiency in MC similar to that in PS-103. Apparently, the PS-103 population contains clones with different degrees of anchorage independence.
D000818:Animals; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002999:Clone Cells / Q000473:pathology; D005549:Foreign-Body Reaction / Q000150:complications; D051379:Mice; D008808:Mice, Inbred CBA; D010969:Plastics; D012513:Sarcoma, Experimental / Q000209:etiology / Q000473:pathology*
D010969:Plastics
Nezavisimost' razmnozheniia ot substrata v neklonirovannoĭ i klonovoĭ populiatsiiakh opukholevykh kletok.