Probable clonal development of foreign-body-induced murine sarcomas.
The number of cells from which tumors induced by subcutaneous implantation of foreign bodies develop was studied in BALB/c mice with X-chromosome-inactivation mosaicism. Because only one of the two X-chromosomes is active in XX somatic cells, a female mouse heterozygous at the X-linked phosphoglycerate kinase (PGK) locus for Pgk-1b and Pgk-1a has two types of cells. In one population Pgk-1b is active and B-enzyme is produced, whereas in the other population Pgk-1a is active and A-type enzyme is synthesized. Normal tissues from these mosaic mice display both enzyme types, but a tumor that develops from a single cell exhibits only one of the two PGK enzyme types. Of 11 sarcomas induced by Millipore filters, 8 showed single-enzyme PGK phenotypes in primary tumors and in tissue cultures derived from them. The 3 other primary sarcomas showed double-enzyme phenotypes. However, transplantation of cultured cells from 2 of these sarcomas resulted in tumors with single-enzyme PGK phenotypes. Thus the data provide confirmatory evidence for the suggestion that most foreign-body-induced sarcomas develop clonally or from a relatively few cells.
D000818:Animals; D002999:Clone Cells / Q000032:analysis*; D005260:Female; D005549:Foreign-Body Reaction / Q000150:complications*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D009030:Mosaicism; D010641:Phenotype; D010735:Phosphoglycerate Kinase / Q000235:genetics; D012509:Sarcoma / Q000209:etiology* / Q000473:pathology; D014960:X Chromosome
D010735:Phosphoglycerate Kinase