Monoclonal antibodies against antigens of the human mammary carcinoma cell line MCF-7.
Mouse monoclonal antibodies against the human mammary carcinoma cell line, MCF-7, were produced and tested against a panel of cell lines. They recognize a variety of intra- and extracellular antigens. Four of them did not react with the human rhabdomyosarcoma cell line A-204 used as a discriminating second target during initial screening. One of these, 45-B/B3, is specific for epithelia, and probably recognizes a cytokeratin. This antibody clearly differentiates carcinomas from sarcomas and lymphomas, and epithelial cells in culture from fibroblasts. It is not species-restricted.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology*; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm