UV-induced imbalance of the deoxyribonucleoside triphosphate pool in E. coli.
The effect of UV irradiation on the intracellular DNA precursor pool in E. coli was investigated. UV irradiation of E. coli, followed by post-incubation for 1-1.5 h, altered the relative sizes of the deoxyribonucleoside triphosphate (dNTP) pool. The total amount of dNTPs increased: both dATP and dTTP increased several-fold, dCTP about twofold, while dGTP remained almost unchanged. In recA- and umuC- strains, which are defective in UV-induced mutagenesis, the pattern of nucleotide pool alterations was similar to that of wild-type strains.
D004261:DNA Replication / Q000528:radiation effects; D003854:Deoxyribonucleotides / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000254:growth & development / Q000528:radiation effects*; D009154:Mutation; D014466:Ultraviolet Rays
D003854:Deoxyribonucleotides