Effects of bovine serum albumin on antibody determination by the enzyme-linked immunosorbent assay.
The effect of bovine serum albumin (BSA) post-coating and addition of BSA to serum diluent was studied in an IgG and IgA Candida antibody enzyme-linked immunosorbent assay (ELISA). BSA post-coating decreased the background readings and increased the specific Candida antibody activity in most sera. However, in a few sera post-coating alone increased background readings and this effect was probably due to the occurrence of BSA antibodies. It could be abolished by the addition of BSA to serum diluent. It is therefore suggested that BSA post-coating should only be used in combination with BSA addition to serum dilution buffer in ELISA.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000908:Antibodies, Fungal / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D002176:Candida albicans / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000908:Antibodies, Fungal; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine