Antibodies of predetermined specificity against chemically synthesized peptides of human interleukin 2.
This communication describes the preparation of antibodies to human interleukin 2 (IL-2), using as immunogens synthetic peptides derived from the predicted amino acid sequence of IL-2. Rabbits and mice were immunized with protein carrier conjugates of eight chemically synthesized IL-2-derived peptides, each consisting of 13-15 amino acids. The immune antisera were screened in a solid-phase ELISA for reactivity to a native human IL-2. Antibodies to four of the eight peptides were found by a variety of biological and immuno-chemical criteria to react against human IL-2. Furthermore, an affinity-purified antibody to one of the IL-2 peptides (peptide 84) specifically stained the cytoplasm of phytohemagglutinin-stimulated human peripheral blood lymphocytes or T-leukemic cells (Jurkat). Antibodies to synthetic IL-2 peptides should serve as useful probes for studying this lymphokine and for developing quantitative assays for measuring its levels in biological fluids and its association with disease.
D000936:Antigen-Antibody Complex; D000941:Antigens / Q000302:isolation & purification; D002051:Burkitt Lymphoma; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis; D013601:T-Lymphocytes / Q000276:immunology
D000936:Antigen-Antibody Complex; D000941:Antigens; D007376:Interleukin-2; D010455:Peptides