Effect of storage of the du Pont lysis-centrifugation system on recovery of bacteria and fungi in a prospective clinical trial.
A commercially available lysis-centrifugation system was compared with a conventional biphasic brain heart infusion medium in a prospective clinical study of 5,125 fungal blood cultures. Recovery rates were compared between two time periods to assess the effect of 25 degrees C storage before processing by the lysis-centrifugation system. The lysis-centrifugation tubes processed within 9 h showed a significantly higher yield (3.4 versus 1.49%) for yeasts (Candida glabrata), filamentous fungi (Histoplasma capsulatum), and bacteria (8.84 versus 7.34%) (Klebsiella pneumoniae and Serratia marcescens) than did those processed after 9 h.
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002498:Centrifugation; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D009181:Mycoses / Q000097:blood* / Q000382:microbiology; D011446:Prospective Studies; D018805:Sepsis / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors