title: 5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.
abstract: We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methylation within this yeast DNA; i.e., there is less than 1 5-methylcytosine per 3,100 to 6,000 cytosine residues.
mesh_terms: D044503:5-Methylcytosine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004271:DNA, Fungal / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000032:analysis*
chemical_list: D004271:DNA, Fungal; D044503:5-Methylcytosine; D003596:Cytosine