title: The alpha-subunit of mouse 7 S nerve growth factor is an inactive serine protease.
abstract: Tryptic and cyanogen bromide peptides accounting for approximately 85% of the amino acid sequence of the alpha-subunit of mouse 7 S nerve growth factor have been isolated and extensively sequenced. The partial structure revealed a high degree of identity with the gamma-subunit (greater than 80%), which is an arginine esteropeptidase of the serine protease family. However, the alpha-subunit does not cleave synthetic arginine ester or peptide substrates nor is it labeled by diisopropylfluorophosphate. The lack of catalytic activity may result from a Gly----His substitution near the active site serine or from the blocked NH2 terminus.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003488:Cyanogen Bromide; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D007610:Kallikreins / Q000302:isolation & purification; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D012697:Serine Endopeptidases; D013363:Submandibular Gland / Q000201:enzymology*; D014357:Trypsin
chemical_list: C013614:7S nerve growth factor complex; D000596:Amino Acids; D046911:Macromolecular Substances; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010450:Endopeptidases; D007610:Kallikreins; D012697:Serine Endopeptidases; D014357:Trypsin; D003488:Cyanogen Bromide