title: Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: a review of methods and applications.
abstract: Proteins and other macromolecules can be injected into cultured cells by several different methods. Here we review the strengths and limitations of two of these methods, red-cell-mediated microinjection and osmotic lysis of pinosomes, and indicate how they may be successfully applied to the study of cultured cells.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004705:Endocytosis; D004910:Erythrocyte Membrane; D008561:Membrane Fusion / Q000187:drug effects; D008845:Microinjections / Q000379:methods*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000494:pharmacology; D011506:Proteins / Q000008:administration & dosage
chemical_list: D011506:Proteins; D011092:Polyethylene Glycols