Effect of X-ray contrast media on the action of chymopapain on the intervertebral disc: an in vitro study of cartilage degradation.
A representative selection of X-ray contrast media was tested for their effects on the action of chymopapain on cartilage proteoglycan in an in-vitro test system. Inhibition of the action of chymopapain was detected only at high concentrations of contrast media, and was more marked with ionic contrast media than with non-ionic media. A dose-response curve for the release of glycosaminoglycan by chymopapain was linear when the amount of enzyme was plotted on a logarithmic scale against glycosaminoglycan release. In view of our results, we suggest that there is no need for a delay between injection of contrast medium and enzyme, contrary to the instructions of the distributors of chymopapain, if small amounts of a non-ionic contrast medium are used.
D000818:Animals; D002417:Cattle; D002917:Chymopapain / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003287:Contrast Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000187:drug effects; D007472:Iohexol; D007477:Ions / Q000494:pharmacology; D007479:Iopamidol; D007482:Iothalamate Meglumine / Q000494:pharmacology; D007483:Iothalamic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D009300:Nasal Septum / Q000187:drug effects*; D014283:Triiodobenzoic Acids / Q000494:pharmacology
D003287:Contrast Media; D006025:Glycosaminoglycans; D007477:Ions; D014283:Triiodobenzoic Acids; D007483:Iothalamic Acid; D007472:Iohexol; D010450:Endopeptidases; D002917:Chymopapain; D007479:Iopamidol; D007482:Iothalamate Meglumine