The effect of phalloidin on insulin secretion from islets of Langerhans isolated from rat pancreas.
Phalloidin, which stabilizes F-actin, has no effect on insulin secretion from intact islets, but penetrates and increases secretion from islets previously made permeable using a high voltage discharge technique. Use of this highly specific drug strongly suggests a role for microfilaments composed of F-actin in the insulin secretory process.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009842:Oligopeptides / Q000494:pharmacology*; D010477:Perfusion; D010590:Phalloidine / Q000494:pharmacology*; D051381:Rats
D007328:Insulin; D009842:Oligopeptides; D010590:Phalloidine; D002118:Calcium