Oligonucleotide mutagenesis of the lacPUV5 promoter.
Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.
D001482:Base Composition; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics*; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides