Cultivation of a porcine adenovirus in porcine thyroid cell cultures.
The porcine adenovirus type 4 was adapted to grow in porcine thyroid cell cultures. A readily recognizable cytopathic effect appeared in these cells as soon as the first passage of the virus and complete degeneration of the monolayers was obtained after only 72 hours post-infection at the fourth passage. A viral yield of 10(6.0) TCID50/ml was calculated after the third passage. The virus was purified by CsCl density gradient centrifugation and was shown to possess a buoyant density of 1.33 g/ml. A specific antiserum was prepared from two specific-pathogen-free piglets and used for indirect immunofluorescent staining. The fluorescence was observed in the nucleus of infected cells at 24 to 72 hours post-inoculation. The use of TP cells is suggested for routine porcine adenovirus diagnosis.
D000256:Adenoviridae / Q000254:growth & development* / Q000302:isolation & purification; D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D013552:Swine; D013961:Thyroid Gland; D014776:Virus Cultivation / Q000379:methods*