Binding of the enzymes of fatty acid beta-oxidation and some related enzymes to pig heart inner mitochondrial membrane.
The binding of crotonase (enoyl-CoA hydratase), beta-hydroxyacyl-CoA dehydrogenase, beta-ketothiolase, succinyl-CoA transferase, and carnitine acetyltransferase to inner mitochondrial membranes, mitoplasts, intact mitochondria, erythrocyte membranes, and phosphatidylcholine liposomes was studied. Succinyl-CoA transferase does not bind to any of these membranes. Carnitine acetyltransferase, on the other hand, binds to all of these membranes. The enzymes of fatty acid beta-oxidation, thiolase, crotonase, and beta-hydroxyacyl-CoA dehydrogenase bind to inner membrane, but not to liposomes. The binding shows a moderate dependence on ionic strength (2-200 mM) and pH (6.5-8). These data indicate the possibility of an organization of the enzymes of fatty acid beta-oxidation on the inner mitochondrial membrane, but do not support the idea of an organization of the enzymes of ketone body catabolism.
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology*; D008929:Mitochondria, Heart / Q000201:enzymology*; D010084:Oxidation-Reduction; D013389:Succinate-CoA Ligases / Q000378:metabolism; D013552:Swine
D005227:Fatty Acids; D000429:Alcohol Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D000426:Alcohol Dehydrogenase; D000102:Acetyl-CoA C-Acyltransferase; D002332:Carnitine O-Acetyltransferase; D004746:Enoyl-CoA Hydratase; D013389:Succinate-CoA Ligases