Occurrence and loss of alkali-labile sites in newly synthesized DNA of UV-irradiated Escherichia coli K12.
In pulse-labelled DNA of ultraviolet-irradiated E. coli, alkali-labile sites were detected. They do not occur in undamaged cells. These sites are produced in wild-type cells as well as in uvrA, uvrB and recA derivatives. Restoration of the synthesis of DNA molecules free of alkali-labile sites requires recA products and involves also uvrA and uvrB products. The chemical nature of alkali-labile sites and their biological function are obscure. They might be stretches of RNA that traverse the lesions, blocking DNA replication and priming recA-dependent DNA replication.
D004261:DNA Replication / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D014466:Ultraviolet Rays
D004269:DNA, Bacterial