title: Antigen beads: a novel approach for the delivery of a soluble Anaplasma immunogen.
abstract: An important consideration in the development of effective immunization regimens against bovine anaplasmosis is the optimization of vaccine delivery systems. This report describes the formulation of proteinaceous antigen-containing spheres derived from supernatant fluids of Analplasma marginale cultures. The antigen beads were designed for use as potential dispersal agents for a soluble Anaplasma immunogen. Immunospecificity assays demonstrated that A marginale antigen was located on the surfaces of individual antigen beads. Use of Anaplasma antigen beads with a potent adjuvant, such as saponin, was proposed.
mesh_terms: D000276:Adjuvants, Immunologic; D000709:Anaplasma / Q000276:immunology*; D000712:Anaplasmosis / Q000517:prevention & control; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007120:Immunochemistry; D012503:Saponins; D012995:Solubility
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007106:Immune Sera; D012503:Saponins