Purification of Eimeria sporozoites by DE-52 anion exchange chromatography.
An anion exchange column of DE-52 has been used to purify Eimeria sporozoites from a post- excystation mixture of oocysts, oocyst shells, sporocysts, sporocyst shells, and sporozoites. The mean recovery from several experiments was 94% and virtually all non-sporozoite material was removed. Infectivity studies in vitro with sporozoites showed that they were viable after purification and were at least as infectious as the unpurified sporozoites; furthermore, oocysts in the crude preparation could be recovered from the DE-52 cellulose by resuspending them in a 20% (w/v) sodium chloride solution.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002645:Chickens / Q000469:parasitology; D002852:Chromatography, Ion Exchange; D004539:Eimeria / Q000254:growth & development / Q000302:isolation & purification*; D007668:Kidney; D010961:Plasmodium / Q000302:isolation & purification; D014349:Trypanosoma cruzi / Q000302:isolation & purification