Evaluation of the API 20E and Microbact 24E systems for the identification of Pseudomonas pseudomallei.
One-hundred isolates of Pseudomonas pseudomallei were used to evaluate the API 20E and Microbact 24E rapid identification systems. The API 20E system identified 50% of the isolates using the revised 1979 Manual only, and 63% when referral was made to the computer centre. A higher identification rate (69 and 87%, respectively) was achieved with a longer incubation period of 96 h. The Microbact 24E system identified 84% of the isolates as P. pseudomallei using the revised 1983 Manual, and 100% when referral was made to the computer centre. The Microbact 24E system would appear to be a reliable system for the identification of P. pseudomallei.
D000818:Animals; D001431:Bacteriological Techniques; D003201:Computers; D005947:Glucose; D006801:Humans; D008751:Methylene Blue; D009862:Online Systems; D011549:Pseudomonas / Q000302:isolation & purification*; D012988:Soil Microbiology; D014871:Water Microbiology
D005947:Glucose; D008751:Methylene Blue