D-glucose anomeric preference of hexokinases from animals and yeast.
The D-glucose anomeric preference of hexokinases partially purified from animals (rat, mouse, and chicken) and baker's yeast (Saccharomyces cerevisiae) were investigated by the assay system with glucose-6-phosphate dehydrogenase as a coupling enzyme. With low Km hexokinases in animal tissues and cells, the ratios of Vmax for the beta-anomer to Vmax for the alpha-anomer (V beta/V alpha) were within a range from 1.3 to 1.5. In yeast, the V beta/V alpha value was 1.1 for hexokinase A, 0.8 for hexokinase B, and 1.4 for glucokinase. The possible explanation for D-glucose anomeric preference of hexokinase is discussed.
D000818:Animals; D002645:Chickens; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013045:Species Specificity; D013237:Stereoisomerism; D013379:Substrate Specificity
D006593:Hexokinase; D005947:Glucose