The identification of a tissue-restricted plasma membrane marker in Xenopus laevis embryos by using a monoclonal antibody.
A library of monoclonal antibodies raised against partially purified membrane fractions from Xenopus laevis oocytes has been produced. One of these antibodies has been cloned and characterized in detail. It was found to be specific for a membrane-bound antigen of apparent Mr, 55,000. The distribution of the antigen has been studied by indirect immunofluorescence on sections of X. laevis embryos and has been found to be highly specific for some ectodermal and endodermal tissues. It was not present on mesodermal tissues.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis*; D004475:Ectoderm / Q000032:analysis; D004625:Embryo, Nonmammalian; D004707:Endoderm / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D008648:Mesoderm / Q000032:analysis; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis; D014018:Tissue Distribution; D014981:Xenopus
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface