A mutational hot spot in the incompatibility gene incC of mini-F plasmid.
The incC incompatibility locus of the mini-F plasmid is also a replication control locus. We have selected and sequenced six independent incC mutations. Each mutation causes a partial loss of incompatibility. All mutations are GC----AT transitions. Five of six mutations occur in the same base pair located at coordinate 46.070 kb; the sixth mutation occurs in an adjacent base pair at 46.069 kb. Both mutational sites are in a hexanucleotide sequence common to several origins of replication.
D001483:Base Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D005144:F Factor; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D009154:Mutation