title: Amino acid sequence of Japanese horseshoe crab (Tachypleus tridentatus) coagulogen B chain: completion of the coagulogen sequence.
abstract: The complete amino acid sequence of the B chain derived from Tachypleus tridentatus coagulogen was determined. It consisted of a total of 129 amino acid residues with a NH2-terminal glycine and COOH-terminal phenylalanine. Sequence studies of the whole B chain and the fragments obtained from the digests with trypsin, alpha-chymotrypsin, thermolysin and Staphylococcal protease V8 showed the following sequence: (sequence; see text) These structural studies of the B chain and the previously established amino acid sequences of the A chain and peptide C derived from T. tridentatus coagulogen, now make it possible to complete the whole sequence of coagulogen consisting of 175 amino acid residues with the molecular weight of 19,723.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification; D002918:Chymotrypsin; D010450:Endopeptidases; D005260:Female; D006737:Horseshoe Crabs; D008297:Male; D010446:Peptide Fragments / Q000032:analysis; D012697:Serine Endopeptidases; D013820:Thermolysin; D014357:Trypsin
chemical_list: D001798:Blood Proteins; D010446:Peptide Fragments; C013445:coagulogen; D010450:Endopeptidases; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C028344:glutamyl endopeptidase; D014357:Trypsin; D013820:Thermolysin