title: Elastin biosynthesis in chick-embryo arteries. Studies on the intracellular site of synthesis of tropoelastin.
abstract: Electrophoretic analyses of the products of cell-free translation of elastin mRNA isolated from 17-day chick-embryo thoracic arteries have demonstrated that the elastin mRNA codes for polypeptides that are slightly larger than the cellular tropoelastin polypeptides synthesized and secreted by matrix-free artery cells. Pulse-chase experiments with cells labelled with [3H]proline established that newly synthesized tropoelastin polypeptides were associated solely with membrane-bound particulate fractions. Cell-free translation of membrane-bound and free polyribosomes isolated from artery cells revealed that the tropoelastin mRNA was associated predominantly with the membrane-bound fraction. When rough-microsomal fractions, isolated from cells labelled with [3H]proline for 10 min, were treated with proteinases in the presence and in the absence of detergent, the nascent tropoelastin polypeptides were shown to be susceptible to proteolysis only when the integrity of the membranes was destroyed by detergent treatment. In similar experiments tropoelastin polypeptides synthesized by membrane-bound polyribosomes in the nuclease-treated reticulocyte lysate were also resistant to the proteolytic-enzyme treatment. The results suggest that tropoelastin polypeptides are synthesized on membrane-bound polyribosomes and discharged into the lumen of the endoplasmic reticulum with co-translational removal of a signal peptide.
mesh_terms: D000818:Animals; D001158:Arteries / Q000378:metabolism*; D002474:Cell-Free System; D002642:Chick Embryo; D004549:Elastin / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007424:Intracellular Fluid / Q000378:metabolism; D010447:Peptide Hydrolases; D010455:Peptides / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014333:Tropoelastin / Q000096:biosynthesis
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D014333:Tropoelastin; D004549:Elastin; D010447:Peptide Hydrolases