Immunoelectronmicroscopic profile of intracytoplasmic immunoglobulins in B non Hodgkin's malignant lymphomas. Comparison with the normal pattern of B lymphoid cells.
An immunoelectronmicroscopic method using Fab fragment of anti human IgG (H + L) has been employed to study the localization of cytoplasmic immunoglobulins in the tumoral cells of 12 B non Hodgkin's malignant lymphomas (B-M.L.). A comparison with normal homologous B lymphoid cells was performed on 10 non tumoral reactive lymph nodes. Immunostaining was observed in PNC, RER and Golgi complex. The criterions of differentiation were discussed in the different B-M.L.. Because of a granular hyaloplasmic immunostaining in normal and tumoral centroblasts and immunoblasts, monospecific antibodies against gamma, mu, alpha heavy chains were used to rule out a non specific uptake. Presence of mu heavy chain was discussed as an argument for immunoglobulin free ribosomal synthesis.
D001402:B-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D003593:Cytoplasm / Q000276:immunology*; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology / Q000648:ultrastructure; D008223:Lymphoma / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010950:Plasma Cells / Q000648:ultrastructure
D007136:Immunoglobulins