Differentiation of presumptive lens ectoderm of the chick in vitro studied with immunofluorescence techniques.
The differentiation capacity of presumptive lens ectoderm was studied in the chick by an in vitro technique using the appearance of central nervous system or lens-specific antigens as indicators of differentiation. Handling the explants resulted in 'autodifferentiation' of both antigens, but co-culture with alcohol-killed primitive node or optic cup material could induce much stronger differentiation. Little specificity exists in the reaction and a hypothesis is presented whereby selection between the two differentiation pathways is thought to be due mainly to maturation within the ectoderm and the inducing tissue plays a minor qualitative role.
D000818:Animals; D001921:Brain / Q000276:immunology; D002454:Cell Differentiation; D002642:Chick Embryo; D004475:Ectoderm / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007908:Lens, Crystalline / Q000196:embryology* / Q000276:immunology; D009924:Organ Culture Techniques
D007106:Immune Sera