Optimizing the lymphocyte transformation test in whole blood. I. Optimum conditions for thymidine incorporation.
The correlation between mitogen concentration and H3-thymidine incorporation can be described mathematically by a sigmoid dose-response curve. Thymidine incorporation/lymphocyte (Ti/Ly) was calculated to facilitate comparison of results. It was highest at a blood dilution of 1:50, lower at 1:10 or 1:250. Ti/Ly obtained with optimum concentrations of PHA was higher than with ConA. Advantages of this optimized method for whole blood over Ficoll-separated mononuclear cells are the higher Ti/Ly, the smaller quantities of blood required and the lower experimental effort.
D002469:Cell Separation; D002478:Cells, Cultured; D003208:Concanavalin A; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007158:Immunologic Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000502:physiology*; D010835:Phytohemagglutinins; D013936:Thymidine
D010835:Phytohemagglutinins; D003208:Concanavalin A; D013936:Thymidine