Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli.
Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and lactose fermentation is read directly from MacConkey agar. It was found that 4-methylumbelliferyl-beta-D-glucuronide could be incorporated directly into a modified MacConkey agar to directly detect the presence of beta-glucuronidase. Other characteristics of MacConkey agar were not affected. The incorporation of 4-methylumbelliferyl-beta-D-glucuronide into modified agar obviated the need for manufacture, quality control, and incubation of reagent-containing test tubes. The time needed to identify E. coli strains was reduced from 1 h to 5 min, and the ability to detect this species in mixed specimens was also enhanced.
D000362:Agar; D003470:Culture Media; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D005453:Fluorescence; D005965:Glucuronates / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D005965:Glucuronates; D007211:Indoles; C002730:4-nitrophenylglucuronide; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; C030374:indole; D000362:Agar; D010088:Oxidoreductases; D005966:Glucuronidase; D007785:Lactose