title: Morphology, kinetics and secretory activity of antibody-forming cells.
abstract: Specific antibody-forming cells from spleen, bone marrow and popliteal lymph nodes were studied in mice after subcutaneous priming and intravenous boosting with horseradish peroxidase (HRP). Functional antibody-secreting capacity of these cells was correlated with their morphology at the cell population level. For this purpose, cells synthesizing anti-HRP antibody from the same cell suspensions were studied simultaneously by light and electron microscopy and by a plaque assay. It appeared that the population of cells responsible for antibody synthesis as well as antibody secretion was morphologically heterogeneous: besides plasma cells, considerable numbers of antibody-forming lymphocytes, antibody-forming plasmablasts and antibody-forming immature plasma cells were observed. Immature plasma cells constituted the majority of antibody-forming as well as antibody-secreting cells. Among the immature plasma cells in the popliteal lymph nodes proliferation occurred. Evidence is presented that the light-microscopically identified mature plasma cell is not the main antibody-forming cell. It does not show 3H-Thymidine incorporation and should be considered as a non-dividing end-cell.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D001345:Autoradiography; D001853:Bone Marrow / Q000276:immunology / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002455:Cell Division; D005260:Female; D006735:Horseradish Peroxidase / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008808:Mice, Inbred CBA; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure
chemical_list: D006735:Horseradish Peroxidase