Monoclonal antibody 2-2-B kills K1-positive Escherichia coli in conjunction with cord blood neutrophils and sera, but not with spinal fluid.
In conjunction with 400 ng/ml murine monoclonal antibody of the IgM class, cord neutrophils were able to kill K1-positive Escherichia coli in an in vitro opsonophagocytic assay. Neither adult nor cord neutrophils mediated bacterial killing in the absence of this monoclonal antibody. This bactericidal capacity was observed with eight of 11 (73%) but not all cord neutrophil samples, and under conditions of bacteria to neutrophil ratios as high as 15:1. Increasing the monoclonal antibody concentration up to 12 micrograms/ml paradoxically resulted in significantly lower amounts of bacterial killing. All 11 cord serum samples had sufficient complement activity to permit this monoclonal antibody to function; however, neonatal cerebrospinal fluid was not an effective complement source. We conclude that this monoclonal antibody may be a useful adjunct to conventional therapy of invasive disease caused by K1-positive E. coli; but it would have to work at the bacteremic phase of infection.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000942:Antigens, Bacterial; D016667:Bacterial Capsules; D001770:Blood Bactericidal Activity; D002555:Cerebrospinal Fluid / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1