Primary structure of the Saccharomyces cerevisiae GAL4 gene.
The GAL4 gene encodes a positive regulator of the galactose-inducible genes in Saccharomyces cerevisiae. Recently, GAL4 has been cloned and its 2.8-kilobase mRNA has been identified. We report here the DNA sequence of GAL4 and the mapping of the 5' and 3' ends of its transcripts. The region sequenced contains a single open reading frame, 881 codons long, which could encode a 99,350-dalton protein. The 5' ends of the GAL4 transcripts fall into two clusters. Transcripts which begin at the upstream cluster would encode the 99,350-dalton protein, whereas those starting at the downstream cluster may result in the synthesis of a shorter, 91,600-dalton protein. The putative GAL4 proteins contain an amino acid sequence near their amino termini which resembles a DNA-binding motif found in bacterial and phage repressors and gene activator proteins.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000302:isolation & purification; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D005690:Galactose / Q000378:metabolism*; D005800:Genes, Fungal; D012331:RNA, Fungal / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D005690:Galactose