Preliminary crystallographic data for a monoclonal Fab fragment specific for HPr of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli.
Crystals for Fab fragments from a monoclonal antibody to HPr of the phosphoenopyruvate:sugar phosphotransferase system of Escherichia coli have been obtained from 14% polyethylene glycol 6000, 5 mM-Tris X HCl, 50 mM-sodium phosphate and 0.2 M-sodium chloride at pH 8.0. The space group is P2(1) with a = 110.85 A, b = 66.18 A, c = 67.21 A, beta = 113.0 degrees and Z = 4. The crystals exhibit the forms [100], [011] and [011] and the solvent content is 47%.
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology*; D003461:Crystallography; D004926:Escherichia coli / Q000032:analysis*; D007140:Immunoglobulin Fab Fragments; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007140:Immunoglobulin Fab Fragments; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr