Evaluation of a fluorogenic assay for detection of Escherichia coli in foods.
A fluorogenic assay procedure with 4-methylumbelliferyl-beta-D-glucuronide incorporated into lauryl sulfate broth was evaluated to detect and confirm the presence of Escherichia coli in foods. Fluorescence is indicative of the presence of E. coli; extensive biochemical confirmation is unnecessary with this assay. The 4-methylumbelliferyl-beta-D-glucuronide assay was tested concurrently with our present methodology for detection of E. coli on 270 samples of raw ingredients and powdered food products. Total agreement between the two methods was 94.8%; there was a false-positive rate of 4.8% and no false-negatives. We found the 4-methylumbelliferyl-beta-D-glucuronide assay to be rapid, accurate, simple to perform, and inexpensive.
D004925:Escherichia / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D005516:Food Microbiology; D006923:Hymecromone / Q000031:analogs & derivatives; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide