title: The effect of Escherichia coli endotoxin and culture filtrate on the lactating bovine mammary gland.
abstract: The pathogenesis of coliform mastitis was studied by observing pathological changes in lactating glands after infusion of either endotoxin or the sterile culture filtrate (CCF) of the medium in which Escherichia coli strain B117 had been grown. Both infusions produced a rapid and intense inflammatory response by 4 h with a marked increase of serum proteins in the milk. Before dispersing into the milk, neutrophils were attached to the ductular epithelium; highest cell counts in the milk were recorded when the tissue reaction had waned. Oedema of the ductular epithelium occurred, particularly where neutrophils were actively migrating. The infusion of CCF produced, in addition to inflammation, degeneration and necrosis of ductular cells. The smallest lesions healed very rapidly. There was evidence of differing cell susceptibility to the necrotising toxin as well as uneven distribution over the epithelial surface. All changes observed were confined to the regions of the teat and lactiferous sinuses with little effect on the secreting tissue. The role of the necrotising toxin in the natural disease remains undetermined.
mesh_terms: D000818:Animals; D002417:Cattle; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000473:pathology*; D008414:Mastitis, Bovine / Q000209:etiology* / Q000473:pathology; D009336:Necrosis; D009504:Neutrophils; D011247:Pregnancy
chemical_list: D004731:Endotoxins