Comparison of chemical analyses of hollow-fiber dialyzer extracts.
Hollow-fiber hemodialyzers containing cellulose-based membranes have been shown to produce positive results with the Limulus amebocyte lysate (LAL) test. This study was undertaken to determine whether endotoxin was causing the reaction. Rinses from 45 parallel-plate and hollow-fiber dialyzers from eight different manufacturers were tested using three lysates and four LAL methods. In addition, four in vitro cellular methods--human leukocytic pyrogen, lymphocytic activating factor, peritoneal macrophage, and arginase release--were used to evaluate endotoxin activity. The substance causing the reaction was identified using chromatographic methods. Results indicate that the LAL-reactive material is cellulose derived and is not pyrogenic.
D001119:Arginase / Q000032:analysis; D002482:Cellulose / Q000276:immunology; D004731:Endotoxins / Q000032:analysis*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007375:Interleukin-1 / Q000032:analysis; D007688:Kidneys, Artificial; D008033:Limulus Test; D008222:Lymphokines / Q000032:analysis; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000276:immunology; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis; D006435:Renal Dialysis / Q000009:adverse effects*
D004731:Endotoxins; D007375:Interleukin-1; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D008567:Membranes, Artificial; D011506:Proteins; C008270:leukocyte endogenous mediator; D002482:Cellulose; D001119:Arginase