title: Disruptive effects of ethyl alcohol on mitotic chromosome segregation in diploid and haploid strains of Aspergillus nidulans.
abstract: To identify, with certainty, the primary genotoxic effects of ethanol, condidia from diploid strains of Aspergillus nidulans were treated during early germination with ethyl alcohol, and all the resulting segregants from large samples were analysed in detail. Results were identical whether technical grade (95%), or highly purified 'absolute', alcohol was diluted to obtain the effective low levels of ethanol (3-6%). This makes it unlikely that trace contaminants, rather than ethanol itself, caused the observed induced segregation. At the most effective concentrations survival was about 50%, but over half of the colonies were abnormal and showed sectoring phenotypes. Higher concentrations were too inhibitory for growth. In practically all cases, when 'abnormals' were replated, aneuploids of various types were recovered. Most aneuploids were hyperdiploid, including a fraction of simple trisomics, and some were even polyploid types. All showed chromosomal-type segregation in diploid sectors, often segregating for genetic markers of many different chromosomes. Mitotic crossing-over was slightly increased, but probably not induced, since an equally high spontaneous frequency was observed among replated aneuploid types. To eliminate conclusively the possibility that chromosome breakage was the primary effect of ethanol, which might indirectly produce aneuploid-like types, haploid conidia were also treated. Up to 8% abnormals, mainly hyperhaploids, were obtained (at about 20-50% survival). When diploid and haploid strains were treated identically with ethanol in liquid media after a few hours of pregermination, frequencies of abnormals were similar for short treatments, but higher in diploid strains for longer ones (10-20% aneuploids). The abnormal colonies from the haploid strain were replated and visually identified: about 2/3 were typical n + 1 hyperhaploids, and most others were n + 2 or 3 or more, including a few 2n + 1 trisomics. It is concluded that as a primary effect, alcohol interferes with, and probably arrests, mitotic segregation, and causes chromosome missegregation and nondisjunction. In most cases, the resulting nuclei contain increased numbers of chromosomes and show high frequencies of chromosome loss.
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics*; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002875:Chromosomes / Q000187:drug effects*; D004171:Diploidy; D000431:Ethanol / Q000633:toxicity*; D006238:Haploidy; D008938:Mitosis / Q000187:drug effects*; D009152:Mutagenicity Tests
chemical_list: D000431:Ethanol