title: The relationship between yeast cell size and cell division in Candida albicans.
abstract: The mean size and percentage of budded and unbudded cells of Candida albicans grown in batch culture over a wide range of doubling times have been measured. Cell volume decreased with increased doubling time and a nonlinear approach to an asymptotic minimum was observed. When cells were separated by age according to bud scars, each age showed a similar decrease. During each cell division cycle, size increased slowly during both budded and unbudded periods so that each generation was significantly larger than the preceding. There was no difference in size between the parent portion of budded cells and unbudded cells of the same age. Time-lapse photomicroscopy of cells growing on solid medium showed that cells divide asymmetrically with larger parents having a shorter subsequent cycle time than the smaller daughter, although the time utilized for bud formation was similar. When cells were shifted from a medium supporting a low growth rate and small size to a medium supporting a faster growth rate and larger size, both budded and unbudded cells increased significantly in size. As the doubling time increased, both the budded and unbudded portions of parental and daughter cycles increased.
mesh_terms: D002176:Candida albicans / Q000166:cytology* / Q000254:growth & development; D002453:Cell Cycle; D002455:Cell Division; D003470:Culture Media; D007700:Kinetics; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development
chemical_list: D003470:Culture Media