title: Effect of hydrocortisone on skin development in the chick embryo: ultrastructural, immunohistological, and biochemical analysis.
abstract: The effect of hydrocortisone on the development of dorsal skin was analyzed in the chick embryo by (1) transmission electron microscopy, (2) indirect immunofluorescence histology of extracellular matrix components (collagen types I, III, and IV; fibronectin; and laminin), and (3) quantitative determination of collagen content and proline incorporation, between administration of the drug at 6 or 6.5 days and final retrieval of skin pieces at 11 days of incubation. Treatment caused the formation of featherless skin areas which exhibited an early maturation of the epidermis, a uniform distribution of interstitial collagen and rarefaction of fibronectin in the dermal extracellular matrix, and a significant increase of collagen content and proline incorporation in collagen noncollagen proteins, characterized by an increased hydroxyproline-to-proline ratio. The distribution of type IV collagen and of laminin was unchanged. The absence of feather formation in hydrocortisone-induced apteria is interpreted as resulting primarily from an early extinction of epidermal morphogenetic competence, and secondarily from modifications in the amount and distribution of extracellular matrix components in the dermis.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis; D004817:Epidermis / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006854:Hydrocortisone / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D007797:Laminin / Q000032:analysis; D008854:Microscopy, Electron; D011392:Proline / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000196:embryology*; D013997:Time Factors
chemical_list: D007797:Laminin; D003094:Collagen; D011392:Proline; D006909:Hydroxyproline; D006854:Hydrocortisone