Effect of post-irradiation inhibition of protein synthesis on UV-induced mitotic gene conversion in Saccharomyces cerevisiae.
The effect of post-irradiation inhibition of protein synthesis with cycloheximide was studied on UV-induced mitotic gene conversion in yeast. The frequency of UV-induced mitotic gene convertants as well as survival were reduced when post-irradiation protein synthesis was inhibited beyond 8 h. It is concluded that proteins required for mitotic recombination are not induced by UV irradiation and are already present in mitotic cells.
D003513:Cycloheximide / Q000494:pharmacology; D005785:Gene Conversion; D005800:Genes, Fungal / Q000187:drug effects / Q000528:radiation effects*; D008938:Mitosis / Q000528:radiation effects*; D009154:Mutation; D014176:Protein Biosynthesis / Q000187:drug effects / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D014466:Ultraviolet Rays
D003513:Cycloheximide