Platelet freezing for serological purposes with and without a cryopreservative.
We compared the reactivity of fresh platelets with platelets frozen in dimethylsulfoxide (DMSO) or without a cryopreservative by testing agglutinating and non-agglutinating platelet-specific alloantibodies in titration. Frozen platelets, irrespective of the method of preservation, showed no loss of antigenicity as compared with fresh platelets. The yield of platelets frozen without a cryopreservative proved to be higher (80%) than platelets frozen in the presence of DMSO (50%). Platelets sensitized with autoantibodies from patients with autoimmune thrombocytopenia could also be stored by simple freezing in platelet-rich plasma and used for (re)testing.
D001323:Autoantibodies; D001792:Blood Platelets / Q000276:immunology; D001793:Blood Preservation / Q000379:methods*; D004121:Dimethyl Sulfoxide; D005455:Fluorescent Antibody Technique; D005615:Freezing; D006801:Humans; D007518:Isoantibodies; D008297:Male; D010974:Platelet Aggregation
D001323:Autoantibodies; D007518:Isoantibodies; D004121:Dimethyl Sulfoxide