An edeine resistant mRNA-dependent protein synthesis system from a Saccharomyces cerevisiae mutant.
A cell-free protein synthesizing system from a mutant of Saccharomyces cerevisiae translated exogenous mRNA in the presence of 2 microM edeine, while a similar system from wild-type strain was completely inhibited by the drug. The mutant ribosomes showed an affinity for [125I]edeine comparable to the wild-type ribosomes, thereby suggesting that these macromolecules alone were not responsible for the edeine-resistant capacity of the mutant.
D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002474:Cell-Free System; D004486:Edeine / Q000378:metabolism / Q000633:toxicity*; D009154:Mutation; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D012333:RNA, Messenger; D004486:Edeine