Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein.
A purified protein derived from the twisted beta-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer's disease has been isolated by Sephadex G-100 column chromatography with 5 M guanidine-HC1 in 1 N acetic acid and by high performance liquid chromatography. Amino acid sequence analysis and a computer search reveals this protein to have no homology with any protein sequenced thus far. This protein may be derived from a unique serum precursor which may provide a diagnostic test for Alzheimer's disease and a means to understand its pathogenesis.
D000544:Alzheimer Disease / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000682:Amyloid / Q000302:isolation & purification*; D016229:Amyloid beta-Peptides; D000686:Amyloidosis / Q000378:metabolism; D001921:Brain / Q000098:blood supply*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans
D000596:Amino Acids; D000682:Amyloid; D016229:Amyloid beta-Peptides