Erroneous synthesis of ribosomal proteins in amino acid starved E. coli.
The effect of amino acid starvation on the accuracy of translation of ribosomal proteins was analyzed in a stringent (relA+)/relaxed (relA) pair of E. coli strains. The degree of misreading was estimated from the amount of cysteine erroneously incorporated into individual proteins during arginine starvation of bacteria. Illegitimate incorporation of cysteine was found to occur to a significant extent in several proteins from both the small and the large subunits of ribosomes, in either type of strain.
D000596:Amino Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics*; D013462:Sulfur Radioisotopes; D014316:Tritium
D000596:Amino Acids; D012269:Ribosomal Proteins; D013462:Sulfur Radioisotopes; D014316:Tritium