Differential determination of cationic and anionic glutathione S-transferases by enzyme immunoassay.
Specific enzyme immunoassays for cationic and anionic glutathione S-transferases were established using the specific antibodies which were purified by antigen-bound adsorbent column chromatography. The enzyme immunoassay for cationic glutathione S-transferase had high specificity to cationic enzyme, but showed no cross reactivity with anionic one, and vice versa. The recovery of cationic glutathione S-transferase by the enzyme immunoassay was 94.7%, and coefficient of variation for within day and day-to-day precision were 7.8-10.4% and 8.5-12.5%, respectively. The enzyme immunoassay for anionic glutathione S-transferase also had a good recovery and precision. Using these enzyme immunoassays for glutathione S-transferases, sera of various patients were analyzed. Serum cationic glutathione S-transferase was increased in patients with hepatitis and hepatoma, and anionic glutathione S-transferase in serum was increased in patients with liver cirrhosis.
D000818:Animals; D000838:Anions; D002412:Cations; D003429:Cross Reactions; D005260:Female; D005982:Glutathione Transferase / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008054:Lipid Peroxides / Q000097:blood; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D011817:Rabbits
D000838:Anions; D002412:Cations; D008054:Lipid Peroxides; D005982:Glutathione Transferase