Antigen and antibody attachment in ELISA for Aspergillus fumigatus IgG antibodies.
Four commercial antigen extracts of Aspergillus fumigatus were evaluated for use in a rapid enzyme-linked immunosorbent assay (ELISA) for anti-A. fumigatus IgG. Initial binding of both somatic and culture filtrate preparations to a polyvinyl chloride solid phase was concentration dependent and increased with incubation time. Antigen binding to the solid phase was reproducible. Binding of A. fumigatus precipitin-positive serum to bound antigen was rapid. All four A. fumigatus antigens demonstrated similar dose-response curves when tested against pooled sera containing a high titre of A. fumigatus antibodies. Detectable activity in precipitin test-negative sera decreased rapidly with dilution. All the antigen preparations were found to be suitable for ELISA procedures and permit the rapid determination of IgG antibodies to A. fumigatus.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology; D001228:Aspergillosis / Q000175:diagnosis / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis* / Q000378:metabolism; D011817:Rabbits
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D000469:Alkaline Phosphatase