Effect of sulphonylurea derivatives, SPC-703 and tolbutamide, on insulin binding by isolated rat adipocytes.
The effect of oral hypoglycaemic drugs, SPC-703 [n-(p-toluenesulphonyl)-5-methyl-2-pirazoline-1-carbonami de] and tolbutamide on insulin binding by rat adipocytes from epididymal fat pads were studied. SPC-703 and tolbutamide in concentration of 1 mM added in vitro to the suspension of adipocytes had no effect on insulin binding and kinetic parameters of insulin receptors. Daily administration of 300 mg/kg body weight of SPC-703 or tolbutamide for 10 days resulted in 48% and 34% increase of specific binding of insulin by adipocytes, respectively. From the Scatchard plot it appears that the increase of binding resulted from increased affinity of insulin receptors. These results may explain extrapancreatic action of sulphonylurea derivatives.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D007328:Insulin / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D013997:Time Factors; D014044:Tolbutamide / Q000494:pharmacology*
D007328:Insulin; D013453:Sulfonylurea Compounds; D014044:Tolbutamide; D011972:Receptor, Insulin; C009971:glipalamide