Mononuclear cell-mediated enhancement of granulocyte function in man.
Human monocyte-enriched mononuclear cells (MNC) (greater than 95% monocytes) powerfully stimulated the antibody-dependent killing of tumor cells by purified human neutrophils (NE) or eosinophils (EO). The enhancement was observed when the mononuclear cell to granulocyte ratio was as low as 1:100. Media conditioned by MNC also stimulated antibody-dependent killing by NE and EO as well as the uptake and killing of Candida albicans by NE. There was a heterogeneity amongst individuals in the capacity of their MNC to elaborate this factor, and approximately 15% of individuals did not produce detectable factor. Different factors in the MNC supernatant were involved in NE and EO activation. The EO-activating factor (AF) had a m.w. of approximately 27,000 and migrated as a single band on phenyl-Sepharose chromatography. The NE-AF had a m.w. of 22,000 and migrated as two distinct bands on phenyl-Sepharose chromatography. EO-colony-stimulating factor (CSF) co-purified with EO-AF, and granulocyte-macrophage-CSF co-purified with both bands of NE-AF. It is concluded that MNC elaborate CSF-like molecules that powerfully stimulate human granulocyte function.
D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D002176:Candida albicans / Q000502:physiology; D003115:Colony-Stimulating Factors / Q000302:isolation & purification / Q000502:physiology; D004640:Emetine / Q000494:pharmacology; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D015846:Monokines; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D011506:Proteins / Q000502:physiology
D003115:Colony-Stimulating Factors; D015846:Monokines; D011506:Proteins; D004640:Emetine