title: The role of cell-wall associated lipids in the pathogenesis of lesions in experimental murine nocardiosis vs lesions produced by Mycobacterium fortuitum.
abstract: The pulmonary pathogens, Nocardia asteroides and Mycobacterium fortuitum classically produce a markedly different tissue response ranging from the acute suppurative lesion of nocardiosis to the granulomatous disease produced by the Mycobacterium. Both organisms have similar cell-wall associated lipids which have been chemically characterized as types of saturated and unsaturated fatty acids. Earlier studies of virulence factors from M. tuberculosis and other Mycobacteria have shown that much of the host response is due to lipid constituency of the organism cell wall. In order to determine that contribution which the cell-wall associated lipids make in the pathogenesis of nocardiosis produced by N. asteroides and mycobacteriosis due to M. fortuitum, separate lipid fractions were obtained using the Anderson extraction technique as modified by Asselineau (Asselineau, J. 1966. The Bacterial Lipids. Hermann, Paris). These lipid fractions were injected into mice and the lesion development observed. Waxes A and D from the two organisms exhibited distinct differences in tissue response. Wax A from Nocardia produced a pronounced tissue response composed of multiple abscesses, macrophages, and reactive fibrous tissue. Wax A from Mycobacterium showed transient aggregations of polymorphonuclear leukocytes. Mycobacteria-derived wax D elicited a marked granulomatous response which persisted throughout the duration of the study, contrasting with a minimally acute inflammatory response to Nocardia-derived wax D. The phosphatide and soluble-fat fractions also showed aggressive lesions; however, these were similar for both organisms. These results indicate that the differences in tissue response elicited by lipids from N. asteroides and M. fortuitum may reside in wax fractions A and D.
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000032:analysis / Q000502:physiology; D008297:Male; D008563:Membrane Lipids / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D009161:Mycobacterium / Q000032:analysis; D009164:Mycobacterium Infections / Q000209:etiology* / Q000473:pathology; D009617:Nocardia Infections / Q000209:etiology* / Q000473:pathology; D009616:Nocardia asteroides / Q000032:analysis / Q000502:physiology
chemical_list: D008563:Membrane Lipids