Plasma levels and biochemical characterisation of circulating met-enkephalin in canine endotoxin shock.
Endogenous opioid peptides have been implicated in the pathophysiology of shock (1-5). In anaesthetised mongrel dogs, administration of E coli endotoxin caused a rise in plasma met-enkephalin-like immunoreactivity (MLI). Biochemical characterisation of MLI by gel filtration chromatography revealed various molecular forms: 31K, 8K, 3-5K and the native pentapeptide in approximately equal amounts. After enzymatic treatment of column fractions the 31K form predominated (90.7%). This is the first demonstration of elevated MLI in endotoxin shock.
D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D002850:Chromatography, Gel; D004285:Dogs; D004744:Enkephalin, Methionine / Q000097:blood* / Q000302:isolation & purification; D004926:Escherichia coli; D005260:Female; D008297:Male; D008970:Molecular Weight; D012772:Shock, Septic / Q000097:blood*; D014357:Trypsin
D004744:Enkephalin, Methionine; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D014357:Trypsin