[Use of molybdenum media with glycerin and admixtures of unassimilated polyols for differentiating actinomycetes].
Actinomycetes were cultivated in a medium containing from 0.15 to 0.2% of ammonium molybdate, glycerol and from 0.25 to 1% of polyol which was not assimilated by the cultures and inhibited the production of molybdenum blue in many actinomycetes. The cultures differed in their susceptibility to the inhibition by various polyols. There were not two polyols that would produce an identical effect on all of the cultures. Correlations were established in the action of polyols. The differences in the formation of molybdenum blue can be used for the differentiation and identification of actinomycetes to subdivide them into groups according to their sensitivity to inositol, mannitol, D-arabitol, xylitol, sorbitol, L-arabitol and dulcitol and according to their resistance to dulcitol (minimal, average and maximal resistance). The paper presents schemes for subdividing groups into subgroups and for establishing the properties.
D000192:Actinomycetales / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D008982:Molybdenum / Q000378:metabolism*; D012988:Soil Microbiology; D013402:Sugar Alcohols / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D013402:Sugar Alcohols; C017541:molybdenum blue; D008982:Molybdenum; D005990:Glycerol
Primenenie molibdenovykh sred s glitserinom i primesiami neusvoiaemykh poliolov dlia differentsiatsii aktinomitsetov.