Abbott radiative energy attenuation method for quantifying ethanol evaluated and compared with gas-liquid chromatography and the Du Pont aca.
Quantification of ethanol by a radiative energy attenuation (REA) technique was evaluated and compared with results by gas-liquid chromatography (GLC) and by the Du Pont aca. Within-assay CVs were less than 5.5%. Between-assay CVs ranged from 1.9% to 6.0% for serum and blood controls at concentrations of 0.5, 1.0, and 2.5 g/L. We observed no cross reactivity with methanol, isopropanol, or acetone, and analytical recovery of ethanol from serum averaged 101%. For the three-method comparison we performed parallel determinations of 156 blood, 92 serum, and 54 urine samples containing a wide range of ethanol concentrations. Linear regression analysis of the REA results vs those of GLC or aca yielded the following: for serum, REA = 1.03 GLC -0.03 (r = 0.998), REA = 1.13 aca -0.04 (r = 0.999); for blood, REA = 0.97 GLC + 0.05 (r = 0.994), REA = 0.99 aca + 0.06 (r = 0.996); and for urine, REA = 1.01 GLC -0.03 (r = 0.998). We also discuss the clinical and forensic use of the REA method for ethanol.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases; D001322:Autoanalysis / Q000295:instrumentation; D002849:Chromatography, Gas; D008058:Dihydrolipoamide Dehydrogenase; D000431:Ethanol / Q000032:analysis* / Q000097:blood / Q000652:urine; D005069:Evaluation Studies as Topic; D005454:Fluorescence Polarization / Q000295:instrumentation; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D008838:Microcomputers; D000091402:Prohibitins; D012044:Regression Analysis
C000717331:PHB2 protein, human; D000091402:Prohibitins; D000431:Ethanol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase