An improved bulk purification method for Escherichia coli elongation factor, Ts.
A bulk purification procedure has been designed to maximize the yield of Escherichia coli elongation factor, Ts, with a minimum of effort and time. The enzyme purification is achieved by DEAE-Sepharose and elongation factor Tu-affinity chromatographies. The typical yield is 150 mg/kg of E. coli (B) cells.
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D010445:Peptide Elongation Factors / Q000302:isolation & purification*
D010445:Peptide Elongation Factors; C023512:elongation factor Ts