[Possible role of tRNA1Lys in recognition of pre-mRNA sequences involved in the control of splicing].
Previously we presented some evidence for involvement of the imported tRNA1Lys in splicing the mitochondrial mosaic genes transcripts in yeast. The new data provided here together with our previous results show that tRNA1Lys/transcripts complementarity permits accurate prediction of intron-exon donor boundaries for at least 7 (probably all 9) Dujonian 1st class introns. A computer-assisted search for homologies between mosaic genes transcripts and sequences complementary to tRNA1Lys reveals most of the control sequences previously determined as the sites of 1) cis-acting splicing blocks, 2) conserved RNA sequences and 3) conserved features of transcripts secondary structure. The sequences revealed in our study are as follows: sequences GGGGG and GGCTC in 2nd class introns and less convincing cases are R + E', E + P and IG in 1st class introns. The results obtained here together with the results of other authors suggest a series of (often weak) alternative RNA-RNA interactions needed to regulate and(or) provide a sufficiently error-free mechanism of splicing for the mosaic genes transcripts. A comparison was made of: 1) sequences near the sites of 2 different autocatalytic Tetrahymena pre-rRNA cleavage-ligation events (splicing and cyclization of the intron RNA) and 2) sequences of tRNA1Lys probably contacting donor boundaries of 1st class mitochondrial introns. The comparison reveals an obvious conservation of some basic structure regardless of the different chemical directions of the strands involved. The result suggests a possible catalytic function for the tRNA1Lys probably involved also in recognition and control processes of mitochondrial transcripts splicing.
D001483:Base Sequence; D003062:Codon; D005800:Genes, Fungal; D012326:RNA Splicing; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003062:Codon; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012346:RNA, Transfer, Amino Acyl
Vozmozhnaia rol' tRNK1Lys v uznavanii uchastkov pre-mRNK, imeiushchikh reguliatornoe znachenie dlia splaĭsinga.