Improved method for processing autoradiographs of cells grown on multiwell plates.
A modification of an established procedure for autoradiographic processing of cultured cells is described. This method eliminates the need for pipetting each individual well and also for cutting and dismantling the multiwell plate for slide preparation. In this procedure the entire plate can be processed as a single unit and the cells can be analyzed in situ, thus eliminating the time consuming pipetting and cutting procedures. Furthermore, the entire experiment can be filed without use of additional slides or storage boxes. Hence, this is a simpler, time conserving, and economical way to process large numbers of cultures for thymidine labeling indices.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology*; D003584:Cytological Techniques / Q000295:instrumentation