Maintenance of granuloma macrophages in serum-free medium.
Granulomas were induced by injecting polyacrylamide beads into subcutaneous pouches created by divulsion of the dorsal skin of mice. More than 10(7) phagocytic cells (60% macrophages, 40% polymorphonuclear cells) could be recovered from this granuloma. The separation of the phagocytic cells can be achieved either following sedimentation in Percoll or following the incubation of cells on plastic petri dishes. Phagocytosis of zymosan was observed in macrophages maintained in vitro for 1 month in Eagle's serum-free medium.
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006099:Granuloma / Q000276:immunology / Q000473:pathology*; D007158:Immunologic Techniques; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology / Q000473:pathology
D003470:Culture Media