title: Secretion of elastin in the embryonic chick aorta as visualized by immunoelectron microscopy.
abstract: Recently, significant advances have been made in characterizing the pathway of elastin biosynthesis from the biochemical point of view and a 70,000 dalton protein, designated tropoelastin, appears to be the primary translation product and soluble intermediate of the insoluble elastin. However, relatively little is known concerning the intracellular secretory pathway of tropoelastin. We previously developed an electron microscopic technique using elastin-specific antibody and ferritin-conjugated secondary antibody to identify intracellular elastin and to identify, provisionally, intracellular vesicles containing elastin ( Damiano et al., Conn. Tiss . Res. 8: 185-188, 1981). However, the method did not permit localization of elastin in other intracellular organelles. We now describe an improved post-embedding technique using the peroxidase-antiperoxidase method to detect the primary elastin antibody and have localized elastin in both the endothelial and medial cells of the embryonic chick aorta. Specific staining was visualized in the cisternae of the endoplasmic reticulum, in the Golgi apparatus, and in vesicles forming on the trans side of the Golgi. Some of these smaller vesicles appeared to fuse, forming larger vesicles which may have a storage function. Both types of vesicles were seen fusing with the cell plasma membrane, suggesting that elastin is secreted by an exocytotic process. These results suggest that tropoelastin follows the classical pathway for protein secretion.
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000648:ultrastructure; D002642:Chick Embryo; D004549:Elastin / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009940:Organoids / Q000378:metabolism; D014333:Tropoelastin / Q000378:metabolism
chemical_list: D014333:Tropoelastin; D004549:Elastin