[Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase of skeletal muscle. II. Compartmentalization of the enzymes in muscle mitochondria and their relative binding capacity].
The compartimentation and the relative strength of binding of the enzymes lipoamide dehydrogenase (LIPDH), citrate synthase (CS), and beta-hydroxyacyl-coenzyme A-dehydrogenase (HADH) in mitochondria isolated from bovine muscle (M. sternomandibularis) were studied using the following methods: Availability of the enzymes for proteases before and after opening of the intracrystal line space and after disintegration of the mitochondrial membranes; release of the enzymes after different treatments of the mitochondria: homogenization with phosphate buffer plus Triton X-100; suspension in dest. water and saccharose-tris buffer with and without added digitonin; ultrasonic treatment; freezing and thawing. From the results it can be concluded that the three enzymes are bound to the inner surface of the inner membrane of the mitochondrion, and that the binding strength increases according to the series CS less than HADH less than LIPDH.
D000818:Animals; D002417:Cattle; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D004072:Digitonin / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005615:Freezing; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000494:pharmacology; D011485:Protein Binding
D009124:Muscle Proteins; D008058:Dihydrolipoamide Dehydrogenase; D002950:Citrate (si)-Synthase; D010447:Peptide Hydrolases; D007652:Oxo-Acid-Lyases; D004072:Digitonin
Lipoamiddehydrogenase, Citratsynthase. und beta-Hydroxyacyl-CoA-dehydrogenase des Skelettmuskels. II. Kompartimentierung der Enzyme im Muskel-Mitochondrion und Ermittlung ihrer relativen Bindungsfestigkeit.