Induction of immunoglobulin secretion in cultured human lymphocytes by 4 Staphylococcus aureus strains and their extracts.
Human blood lymphocytes were stimulated in vitro by four Staphylococcus aureus strains. Activation of immunoglobulin-secreting cells was determined by a reverse plaque forming cell (PFC) assay, and proliferation by quantitation of thymidine incorporation. Whole killed S. aureus were slightly more efficient than water-soluble preparations in the form of sonicated extracts and culture supernatants. Two S. aureus strains rich in protein A (Cowan I and E 2371) and one S. aureus strain deficient in protein A (E 1369) were potent B-lymphocyte stimulators inducing maximal activity on day 6 of culture. Another S. aureus strain deficient in protein A (Wood 46) did not possess++ human lymphocyte stimulating capacity.
D006462:Hemolytic Plaque Technique; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008213:Lymphocyte Activation; D012703:Serotyping; D013010:Sonication; D013211:Staphylococcus aureus / Q000145:classification / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D014020:Tissue Extracts