Anti-LPS factor in the horseshoe crab, Tachypleus tridentatus. Its hemolytic activity on the red blood cell sensitized with lipopolysaccharide.
Anti-LPS factor, which inhibits the endotoxin mediated coagulation system in the horseshoe crab, Tachypleus tridentatus, was found to lyse red blood cells sensitized with gram-negative bacterial LPS, but not to lyse unsensitized cells. This hemolysis occurred even at 0 degree C and was completed within 1 min. The binding of anti-LPS factor to LPS must be essential for the hemolysis, because free LPS inhibited the hemolytic action of anti-LPS factor.
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D002645:Chickens; D004912:Erythrocytes / Q000187:drug effects*; D004926:Escherichia coli; D006461:Hemolysis; D006736:Horses; D006737:Horseshoe Crabs / Q000032:analysis*; D006801:Humans; D007447:Invertebrate Hormones / Q000494:pharmacology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D012475:Salmonella; D013696:Temperature
D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D008070:Lipopolysaccharides; C034705:antilipopolysaccharide factor (Limulus)