The use of Hanks'--pipes buffers in the preparation of human, normal leucocytes for TEM observation.
It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy observation of these cells but also for scanning electron microscopy.
D002021:Buffers; D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D006801:Humans; D007962:Leukocytes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
D002021:Buffers