Acetylcholinesterase biosynthesis and transport in tissue culture.
The enzyme acetylcholinesterase consists of a family of molecular forms differing in subunit composition, solubility properties, and subcellular location. The use of a variety of reversible or irreversible active site-directed ligands with different membrane permeability properties permits the selective inactivation of separate pools of enzyme molecules. The application of these inhibitors together with standard biochemical techniques has permitted a detailed characterization of the synthesis and metabolism of the secretory and membrane-bound acetylcholinesterase in tissue-cultured cells. These techniques, with minor modifications and appropriate controls, can also be applied to the study of AChE metabolism in organ culture and in vivo.
D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011865:Radioisotope Dilution Technique
D002250:Carbon Radioisotopes; D000110:Acetylcholinesterase