C3b receptors on the cell membranes of the stimulated mouse macrophages. An immunohistochemical study.
C3b receptors were visualized on the cell membranes of stimulated mouse peritoneal macrophages (Mø) by the incubation with cross-reacting swine C3 with proof of this latter by FITC- or HRP-conjugated rabbit anti-swine-C3 antibody. The FITC-conjugate produced a granular and spotty fluorescence. In ultrastructure, the HRP-conjugate revealed minute dense areas of reaction product, whose modest numbers were seen in both aldehyde-prefixed and non-prefixed cell samples.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003179:Complement C3b / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D011951:Receptors, Complement / Q000032:analysis*
D011951:Receptors, Complement; D003179:Complement C3b