Rabbit antiserum to a citric acid extract of human skeletal muscle staining thymomas from myasthenia gravis patients.
Rabbit antiserum to a citric acid extract of human skeletal muscle (CA) stained both the cell membrane and the cross-striational bands of skeletal muscle cells. The rabbit antiserum also stained the cell membrane of epithelial thymoma cells from myasthenia gravis (MG) patients. Normal and hyperplastic thymus tissue were not stained, apart from scattered myoid thymic cells. Absorption of the antiserum with CA abolished staining of the thymoma, indicating that human skeletal muscle and epithelial thymoma cells possess common antigens.
D000042:Absorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000941:Antigens / Q000276:immunology; D002951:Citrates / Q000276:immunology*; D019343:Citric Acid; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D009132:Muscles / Q000276:immunology*; D009157:Myasthenia Gravis / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D013945:Thymoma / Q000378:metabolism*; D013952:Thymus Hyperplasia / Q000276:immunology; D013953:Thymus Neoplasms / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000941:Antigens; D002951:Citrates; D007106:Immune Sera; D019343:Citric Acid