Preparation of mouse type I and type II insulins for immunologic studies.
Mouse pancreata contain comparatively meager amounts of two insulin species, types I and II. When these insulins are to be prepared for immunogenetic studies, it is desirable to obtain equivalent amounts of both in concentrations suitable for immunization. Standard methods, based on isolating single species, favor recovery of one type. Moreover, published methods for separation of type I from type II produce very dilute insulin solutions. Methods are suggested here to overcome these disadvantages.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D007328:Insulin / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000032:analysis*; D011863:Radioimmunoassay; D013552:Swine
D007328:Insulin