title: Yeast pheromone alpha-factor is synthesized as a high molecular weight precursor.
abstract: The sex pheromone alpha-factor of Saccharomyces cerevisiae, a tridecapeptide of approx. 1,700 molecular weight, was found to be synthesized in vivo as a high molecular weight precursor of Mr = 28,000. Inhibition of N-linked glycosylation by tunicamycin leads to three precursor species of lower molecular weight indicating three carbohydrate residues linked to the alpha-factor precursor molecule. A molecular weight of 18,000 was determined for the unglycosylated molecule.
mesh_terms: D000818:Animals; D000072235:Mating Factor; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification; D010675:Pheromones / Q000235:genetics*; D011498:Protein Precursors / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D010455:Peptides; D010675:Pheromones; D011498:Protein Precursors; D014415:Tunicamycin; D000072235:Mating Factor