Phosphatidylcholine is the major phospholipid providing arachidonic acid for prostacyclin synthesis in thrombin-stimulated human endothelial cells.
Upon incubation for 24 hours with [3H]arachidonic acid (AA, 1 mu Ci/ml), cultured endothelial cells from human umbilical vein incorporated one half of the added radioactivity, mostly into phospholipids (83% of the total cell radioactivity). Distribution of the label between the various phospholipid classes was found to reflect the distribution of endogenous AA. Stimulation with human thrombin (2 U/ml) promoted a rapid release of radioactive material into supernatants, which contained essentially 6-keto-prostaglandin F1 alpha and non-converted AA. This process levelled off at 10 min, at which time phosphatidylcholine displayed a decrease accounting for 3.7% of the total cell radioactivity. Phosphatidylinositol also appeared significantly diminished, but this decrease was almost 2.5 fold less than that observed in phosphatidylcholine. It is concluded that AA availability for prostacyclin biosynthesis is mostly regulated by a phospholipase A2.
D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D004727:Endothelium / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D010713:Phosphatidylcholines / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors; D014471:Umbilical Veins / Q000378:metabolism
D001095:Arachidonic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D011464:Epoprostenol; D013917:Thrombin