Role of the p53 protein in cell proliferation as studied by microinjection of monoclonal antibodies.
Two monoclonal antibodies against the p53 protein, PAb 122 and 200-47, were microinjected into mammalian cells as a probe to determine the role of the p53 protein in cell proliferation. PAb 122 recognizes the p53 proteins of mouse and human cells but not of hamster cells, whereas 200-47 recognizes the p53 proteins of mouse and hamster cells but not of human cells. The ability of these antibodies to inhibit serum-stimulated DNA synthesis of cells in culture correlates with their ability to recognize the species-specific antigenic determinants. More important, however, is the observation that microinjected PAb 122 inhibits the transition of Swiss 3T3 cells from G0 to S phase, but has no effect on the progression of these cells from mitosis to the S phase.
D000165:Acridine Orange; D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002455:Cell Division; D002460:Cell Line; D011232:Chemical Precipitation; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D006801:Humans; D008647:Mesocricetus; D051379:Mice; D008845:Microinjections; D008938:Mitosis / Q000187:drug effects; D010750:Phosphoproteins / Q000276:immunology / Q000502:physiology*; D016159:Tumor Suppressor Protein p53
D000911:Antibodies, Monoclonal; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D004247:DNA; D000165:Acridine Orange