Proteolytic capacities of cystic fibrosis and control fibroblasts towards Tamm-Horsfall glycoprotein.
125I-labelled Tamm-Horsfall glycoprotein was incubated at pH 5.0 and pH 7.4 with homogenates of cultured fibroblasts from cystic fibrosis patients and controls in order to compare the total proteolytic capacities of these cells. No significant breakdown could be observed at pH 7.4. Protein degradation at pH 5.0 occurred at comparable rates in cells from patients and controls, with the possible exception of one cystic fibrosis line. Therefore, a decreased proteolytic capacity cannot be a general cellular characteristic of cystic fibrosis. Though occurrence of heterogeneity cannot be excluded, the exception probably represents just random variation.
D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000201:enzymology*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009088:Mucoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D058949:Uromodulin
D009088:Mucoproteins; C548612:UMOD protein, human; D058949:Uromodulin; D010447:Peptide Hydrolases