Synaptonemal complex-associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique.
An improved method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) is described. This technique produces clear preparations of SCs and, in addition, consistently reveals both centromeres and recombination nodules (RNs) in PTA-stained preparations viewed by electron microscopy. A preliminary study of RN number and distribution in Allium fistulosum indicates that they faithfully reflect the positions of cross-over exchange events as revealed by chiasmata.
D002467:Cell Nucleus / Q000648:ultrastructure*; D002503:Centromere / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D006652:Histological Techniques; D008540:Meiosis; D008854:Microscopy, Electron; D010944:Plants / Q000648:ultrastructure*