[Mutagenesis of amino and/or methyl analogs of acridine in Salmonella and yeast: a comparison between the spot-test and the pre-incubation methods].
The relationship between mutagenic activities and chemical structure of acridine derivatives was examined by using Salmonella typhimurium strains TA 1537 and TA 1977 and yeast Saccharomyces cerevisiae. Most analogs with amino and/or methyl group(s) caused frameshift-type mutation in Salmonella without mammalian microsomal enzyme activation. The 9-amino analogs were strong mutagens and mutagenicity was also increased when 10-position was methylated in 1-amino and 2-amino compounds. However, 9-amino derivatives did not cause mitochondrial mutation in yeast, where 3,6-diamino and/or 10-methyl groups were structural requisites for significant mutagenic activity. In comparison with the pre-incubation method, the spot-test method was shown to be less sensitive. The mutagenicity of some compounds could not be detected by the spot-test method. Mutagenic activities of these drugs were revealed and increased markedly with an increase in pre-incubation period up to 20 min, suggesting that the pre-incubation of tester bacteria with a compound prior to a mutagenicity assay is necessary for the detection of mutagenesis of these compounds.
D000166:Acridines / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics; D013329:Structure-Activity Relationship
D000166:Acridines