Differential effect of the serine protease inhibitor phenyl methyl sulfonyl fluoride on cytochemically detectable esterases in human leucocytes and platelets.
Esterases of human leucocytes and platelets were studied by cytochemical methods. The aim of the study was to clarify the cellular distribution and possible nature of esterases types differing in their substrate specificity and/or their inhibitor sensitivity. 3 substrates (alpha-naphthyl acetate: ANA; naphthol AS-D chloroacetate: NASDCA; and N-acetyl DL-alanine alpha-naphthyl ester: NACALA) were used and the effects of 2 inhibitors (sodium fluoride and the serine protease inhibitor phenyl methyl sulfonyl fluoride: PMSF) were evaluated. 4 enzyme types were described: Type I, present in granulocytes, was detected using NASDCA and NACALA and was resistant to fluoride but sensitive to PMSF. Other types were detected using ANA as substrate. Type II, present in monocytes, was inhibited by both fluoride and PMSF. Type III, present in platelets and plasma cells, was inhibited by fluoride but resistant to PMSF. Type IV, present in lymphocytes, was resistant to both fluoride and PMSF. The specific aims and possible areas for application of these results are discussed.
D000328:Adult; D001792:Blood Platelets / Q000201:enzymology*; D001853:Bone Marrow / Q000201:enzymology; D001854:Bone Marrow Cells; D004950:Esterases / Q000037:antagonists & inhibitors / Q000097:blood*; D006651:Histocytochemistry; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D009283:Naphthol AS D Esterase / Q000097:blood; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D012697:Serine Endopeptidases; D012969:Sodium Fluoride / Q000494:pharmacology; D013379:Substrate Specificity; D013450:Sulfones / Q000494:pharmacology*
D011480:Protease Inhibitors; D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride; D012969:Sodium Fluoride; D004950:Esterases; D009283:Naphthol AS D Esterase; D012697:Serine Endopeptidases