Sequence preferences in the binding to DNA of triostin A and TANDEM as reported by DNase I footprinting.
Six or seven triostin-binding sites have been identified in a 160-base-pair DNA restriction fragment containing the tyr T promoter sequence. Each is centred round a CpG step, and the minimum binding site-size appears to be six base pairs. The sites are practically the same as those reported for echinomycin by DNase I digestion. Only two sites are protected by binding of TANDEM, the des-N-tetramethyl analogue of triostin A; they are centred around the sequences ATA or TAT.
D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004926:Escherichia coli; D011401:Promoter Regions, Genetic; D011810:Quinoxalines / Q000378:metabolism
D004269:DNA, Bacterial; D011810:Quinoxalines; C031945:triostin A; C017738:TANDEM (quinoxaline); D003850:Deoxyribonuclease I