Effects of immunostimulants on the in vitro generation of cytotoxic lymphocytes against cervical cancer cell lines.
Human peripheral blood lymphocytes were sensitized in vitro against cervical cancer cell lines and the effect of immunostimulants, such as BCG, streptococcal preparation (OK-432), yeast cell wall, and Concanavalin A, on the generation of cytotoxic lymphocytes was examined. The cytotoxic activity of sensitized lymphocytes was augmented by the addition of OK-432 (0.01 KE/ml) during in vitro sensitization, but the induction of sensitized lymphocytes was inhibited by the addition of yeast cell wall (10 micrograms/ml). When the lymphocytes from cervical cancer patients free from tumor after surgical operation were stimulated on the autologous tumor cell monolayer, the cytotoxic lymphocytes could be generated. In vitro primed lymphocytes could be reactivated following the stimulation with OK-432 (0.01 KE/ml) for 48 hr and the requirement for a proliferative trigger seems to be important for the in vitro generation of cytotoxic lymphocytes. The role of nonspecific stimulation by LD-like products in the in vitro generation of cytotoxic lymphocytes was discussed.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000946:Antigens, Fungal / Q000494:pharmacology; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D009163:Mycobacterium bovis; D010835:Phytohemagglutinins / Q000494:pharmacology; D010844:Picibanil / Q000494:pharmacology; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000946:Antigens, Fungal; D010835:Phytohemagglutinins; D003208:Concanavalin A; D010844:Picibanil