Reactions of extracts of Reiter treponeme with syphilitic and non-syphilitic human sera in the single radial haemolysis technique.
A single radial haemolysis (SRH) technique, using Reiter protein or Reiter lysate as the coating antigen, was investigated. Results obtained with syphilitic and presumed non-syphilitic human sera were compared with results obtained in the absorbed fluorescent treponemal antibody test (FTA-ABS), the Reiter protein complement fixation test ( RPCFT ), the Venereal Diseases Research Laboratory Slide test (VDRL) and the Cardiolipin Wasserman reaction ( CWR ). The SRH reaction, with either Reiter antigen, was more sensitive than any of the screening tests ( RPCFT , VDRL and CWR ) for detecting positive syphilitic antibodies. Although the SRH test used almost the same materials as the RPCFT , it was appreciably more sensitive for the detection of the group-specific antibodies in syphilitic human serum.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology*; D002645:Chickens / Q000097:blood; D003168:Complement Fixation Tests; D003165:Complement System Proteins; D004912:Erythrocytes; D005455:Fluorescent Antibody Technique; D006462:Hemolytic Plaque Technique; D006801:Humans; D047348:Hydrolyzable Tannins; D012756:Sheep / Q000097:blood; D013587:Syphilis / Q000276:immunology; D013588:Syphilis Serodiagnosis / Q000379:methods*; D013696:Temperature; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D047348:Hydrolyzable Tannins; C009163:Reiter protein, Treponema pallidum; D003165:Complement System Proteins