Biological activity of recombinant human interleukin-2 produced in Escherichia coli.
The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization. No functional differences between native and the recombinant interleukin-2 molecules have been detected.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D007694:Killer Cells, Natural / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic
D004274:DNA, Recombinant; D007376:Interleukin-2