A new strain of Salmonella typhimurium reverted by mitomycin C and N-methyl-N'-nitro-N-nitrosoguanidine--a possible universal tester for mutagenic compounds.
A strain of Salmonella typhimurium, SO1007, which carries the amber mutation trpD28 plus the plasmid pKM101 was reverted very efficiently by two mutagens with different mutagenic specificities and modes of action: mitomycin C (MC) and N-methyl-N'-nitro-N-nitrosoguanidine (NG). By selecting revertants on minimal agar supplemented with anthranilic acid (AA), two distinct phenotypic classes of TrpD28 revertants can be recovered: prototrophs (MM+) and anthranilate utilizers (AA+). Since each phenotypic class is known to be caused by a variety of mutational events, reversion of trpD28 on minimal-anthranilate medium may be useful for detecting mutagenic agents regardless of the types of mutations they may cause. Thus, strains like SO1007 may be useful as 'universal' detectors of mutagenic compounds. In the course of these experiments we also observed that pKM101 does not protect but, on the contrary, sensitizes the host bacteria slightly to the toxic effects of MC.
D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation / Q000187:drug effects; D009876:Operon; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D014364:Tryptophan / Q000235:genetics
D008937:Mitomycins; D008769:Methylnitronitrosoguanidine; D014364:Tryptophan