Cytokine effects on a B-cell line.
The murine pre-B-like cell line, 70Z /3, has been shown to undergo differentiation in response to supernatants derived from concanavalin-A (Con A) stimulated rat spleen cells or ultraviolet light treated P388D1 macrophage cell line. After culture for 24 h with supernatant factors, normally membrane IgM negative 70Z /3 cells are induced to synthesize light chain and become high level membrane IgM expressors . The cytokine (s) responsible for inducing 70Z /3 cells to differentiate is different from interleukin-2 (IL-2), B-cell growth factor (BCGF), colony-stimulating factor (CSF) I and II, but one of the cytokines is either very similar or identical to interleukin-1 (IL-1). These results demonstrate that IL-1 or a molecule with very similar physical properties can act directly on a B-cell line and thus probably also on normal B cells to influence differentiation.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D001688:Biological Products / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines; D007075:Immunoglobulin M / Q000096:biosynthesis; D007375:Interleukin-1 / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D013154:Spleen / Q000276:immunology
D001688:Biological Products; D016207:Cytokines; D007075:Immunoglobulin M; D007375:Interleukin-1; D003208:Concanavalin A