Prostacyclin producing activity of human umbilical, placental and uterine vessels.
Prostacyclin-like material producing activity of umbilical, placental and uterine vessels was studied. Umbilical arteries and veins were separated at sites 10-15cm and 1-2cm from insertion of the umbilical cord to the placenta. Placental arteries and veins were prepared from the first, second and third branches on the chorionic plate. Uterine vessels were obtained at abdominal hysterectomy. After incubation of each specimen in Tris buffer 1 ml (pH8.5, 0.5M) for 30 min at room temperature, the inhibitory effect of the medium on ADP induced platelet aggregation was measured and the prostacyclin-like material was quantified. These procedures were repeated consecutively four times in total for each specimen. Prostacyclin-like material production rate and its total production were calculated. In total prostacyclin-like material production, umbilical arteries and veins were much higher than placental arteries and veins respectively (p less than 0.001), but there was no significant difference between placental and uterine vessels. These results showed that prostacyclin-like material producing activity of blood vessels declined remarkably at the transitive region from umbilical to placental vessels. It seems that this distribution of vascular prostacyclin-like material producibility in the fetoplacental vascular system correlates with that of vascular reactivity to prostacyclin.
D000328:Adult; D001808:Blood Vessels / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D010920:Placenta / Q000098:blood supply*; D010974:Platelet Aggregation / Q000187:drug effects; D011247:Pregnancy; D014469:Umbilical Arteries / Q000378:metabolism*; D014471:Umbilical Veins / Q000378:metabolism*; D014599:Uterus / Q000098:blood supply*
D011464:Epoprostenol; D007213:Indomethacin