title: A desmutagenic factor isolated from burdock (Arctium lappa Linne).
abstract: A desmutagenic factor was isolated from burdock (Arctium lappa Linne). This factor reduced the mutagenicity of mutagens that are active without metabolic activation, such as 4-NO2-1,2-DAB and 2-NO2-1,4-DAB, as well as mutagens such as ethidium bromide, 2-aminoanthracene, Trp-P-1 and Trp-P-2 requiring S9 for metabolic activation. It is resistant to heat and proteolytic enzymes and sensitive to treatment with MnCl2. The partially purified principles had a molecular weight higher than 300 000 and showed characteristics of a polyanionic substance. An irreversible diminution of the mutagen was confirmed by treatment of 2-NO2-1,4-DAB or Trp-P-2 with the burdock factor.
mesh_terms: D001711:Biotransformation; D002413:Cations, Divalent; D006358:Hot Temperature; D009152:Mutagenicity Tests; D009153:Mutagens / Q000037:antagonists & inhibitors*; D009154:Mutation / Q000187:drug effects; D010447:Peptide Hydrolases; D010936:Plant Extracts / Q000494:pharmacology*; D010945:Plants, Edible; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D002413:Cations, Divalent; D009153:Mutagens; D010936:Plant Extracts; D010447:Peptide Hydrolases