title: Ultrastructural localization of carcino-embryonic antigen in a continuous human tumour cell line (LS 174 T) in relation to morphometric investigations.
abstract: The synthesis of carcinoembryonic antigen (CEA) in human tumour cell cultures has been investigated at the ultrastructural level in the colon carcinoma cell line LS 174 T. By application of a CEA-specific antibody fraction, gained from goat serum which was directly coupled with horse radish peroxidase (HRP), the expression of CEA could be followed in the cell culture both intra- and extracellularly with ultra-immunohistochemical methods, utilizing the pre-embedding technique. Morphometric and ultra-immunohistochemical investigations showed the possible involvement of the Golgi systems in CEA production. The changes in the volume fraction of the Golgi apparatus related to the total cell volume showed a direct correlation with the CEA content in the cell culture supernatants. Freeze-etch control experiments revealed negligible structural changes in the cells following ethyl-dimethyl-aminopropylcarbodiimide (EDC)-and saponin permeation techniques.
mesh_terms: D000230:Adenocarcinoma / Q000032:analysis* / Q000648:ultrastructure; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000032:analysis* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D002272:Carcinoembryonic Antigen