Synthesis of basal lamina by epidermal cells in vitro.
In epidermal cell cultures grown on type I collagen, the in vitro synthesis of basal lamina was studied by electron microscopy and indirect immunofluorescence with anti-type IV collagen antibody. The newly-formed basal lamina and type IV collagen were observed in a linear pattern beneath the epidermal cells. The observations strongly suggest that the basal lamina containing type IV collagen is newly synthesized by the epidermal cells.
D001485:Basement Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron
D003094:Collagen