title: [Selection of Streptomyces spheroides--a producer of extracellular proteases with fibrinolytic action].
abstract: The mutagenic action of UV on Streptomyces spheroides 35 spores produced mutants with an elevated biosynthesis of exocellular proteases having the fibrinolytic activity. During submerged cultivation, mutant M8-2 accumulated 4-5 times more fibrinolytic enzymes with a high thrombolytic activity in the cultural broth than the parent strain. The selection of mutants synthesizing proteases with a high fibrinolytic activity can be made more effective by using a medium which contains fibrin, a substrate specific for the enzymes, as a sole source of carbon and nitrogen. The diameters of zones of fibrin hydrolysis around the colonies on this medium are a reliable criterion in the selection of such mutants. The preparation of proteolytic enzymes isolated from the cultural broth of mutant M8-2 is 5.3 and 3.9 times more active in fibrin and casein hydrolysis, respectively, than the preparation of the parent strain. Moreover, it has a high thrombolytic activity.
mesh_terms: D020689:Exopeptidases; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D006868:Hydrolysis; D009154:Mutation; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000494:pharmacology; D012641:Selection, Genetic; D013302:Streptomyces / Q000201:enzymology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D005337:Fibrin; D020689:Exopeptidases; D010447:Peptide Hydrolases
vernacular_title: Selektsiia Streptomyces spheroides--produtsenta vnekletochnykh proteaz s fibrinoliticheskim deĭstviem.