A rapid screening technique for lymphocytotoxic antibodies using tray-frozen lymphocytes.
A method whereby sera can be screened for the presence of lymphocytotoxic antibodies within 4 hr using lymphocytes frozen in microtest trays is described. The reactions of the sera of 48 hemodialysis patients against freshly prepared lymphocytes were compared with those against tube-frozen (384 reaction pairs) and tray-frozen (864 reaction pairs) cells. There was a better than 90% concordance, and only 3% of the reactions differed from negative to strongly positive or vice versa. Preliminary results indicate that the method is also suitable for B-cell (HLA-DR) antibody testing. Routine screening against 50- or 100-cell panels can be accomplished more rapidly and more efficiently using tray-frozen lymphocytes. The data matrix for analysis of a serum's HLA specificity is greatly reduced in comparison to the conventional screening technique, rendering the method particularly suitable for microcomputer processing.
D000961:Antilymphocyte Serum / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D005615:Freezing; D006680:HLA Antigens / Q000276:immunology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II