Insulin binding to erythrocytes in children with type-I diabetes mellitus.
Specific binding of [125I]insulin to isolated erythrocytes was investigated in four groups of children (A) Healthy children under 10 years of age (n = 20) (B) Healthy children over 10 years of age (n = 13) (C) Diabetic children under 10 years of age (n = 12) (D) Diabetic children over 10 years of age (n = 63). In addition, all diabetic children (n = 75) were subdivided into four groups according to the duration of diabetes. (E) less than 2 years, (F) 2--4 years, (G) 4--6 years, and (H) greater than 6 years. By means of a nonlinear regression analysis for the extraction of binding parameters (assuming a single receptor class model with independent receptor sites) and methods of variance analysis, statistically significant differences were observed for the receptor affinities Ka (10(8) l/mol) and the receptor concentrations X0 (nmol/l) between groups A and C, B and D, C and D, but not between A and B. The affinities of groups C and D were found to be higher than the corresponding values of groups A and B, whereas the receptor concentrations exhibited an inverse behaviour. A significant increase of the receptor concentration and decrease of the receptor affinity depending on the duration of diabetes could only be proved to exist during the first 2 years of the disease.
D000293:Adolescent; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives* / Q000097:blood / Q000627:therapeutic use; D007700:Kinetics; D008297:Male; D011972:Receptor, Insulin / Q000378:metabolism*; D012016:Reference Values; D013997:Time Factors
D007328:Insulin; C008147:insulin, iodo-; D011972:Receptor, Insulin