title: Immunological cross-reactivity between carbamyl phosphate synthetases I, II, and III.
abstract: Four types of carbamyl phosphate synthetase have been previously distinguished on the basis of catalytic properties and metabolic role. Immunoblot assay has now demonstrated cross-reactivity between rat liver carbamyl phosphate synthetase I and the following other three types of synthetases: carbamyl phosphate synthetase II from SV40-transformed baby hamster kidney cells, carbamyl phosphate synthetase III from spiny dogfish liver and from largemouth bass liver, and Escherichia coli carbamyl phosphate synthetase. The strongest cross-reactivity was observed between carbamyl phosphate synthetases I and III. These findings indicate at least partial structural homology among the various synthetases and constitute the first demonstration of such a relationship among the enzymes.
mesh_terms: D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000276:immunology*; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000276:immunology*; D002417:Cattle; D006224:Cricetinae; D003429:Cross Reactions; D004926:Escherichia coli / Q000201:enzymology; D005399:Fishes; D006801:Humans; D007163:Immunosorbent Techniques; D007668:Kidney / Q000201:enzymology; D008025:Ligases / Q000276:immunology*; D008099:Liver / Q000201:enzymology; D051381:Rats; D013045:Species Specificity
chemical_list: D008025:Ligases; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)