Titration of antibodies to Paracoccidioides brasiliensis by erythro-immunoassay (EIA).
The erythro-immunoassay, a new serological procedure in which a hybrid antibody conjugate is able to bind erythrocytes, was used for the titration of antibodies against P. brasiliensis in sera from patients with paracoccidioidomycosis. A peptide-polysaccharide and a lyophilized yeast culture filtrate of P. brasiliensis were used as antigens. Absorption with dead Candida albicans whole cells was necessary to decrease cross reactions observed with heterologous sera. Erythro-immunoassay provides a sensitive system for titration of antibodies in paracoccidioidomycosis with serum dilutions up to 1:102000.
D000908:Antibodies, Fungal / Q000032:analysis*; D002176:Candida albicans / Q000276:immunology; D003429:Cross Reactions; D004912:Erythrocytes; D005658:Fungi / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007164:Immunosorbents; D010228:Paracoccidioides / Q000276:immunology*
D000908:Antibodies, Fungal; D007164:Immunosorbents