Multiple forms of intracellular alpha-glucosidase from Thermoascus aurantiacus Miehe: purification and properties.
The intracellular alpha-glucosidase extracted from the mycelium of Thermoascus aurantiacus was resolved by chromatographic procedures on DEAE-cellulose and hydroxylapatite into 4 major forms which account for 96% of the recovered activity. These forms, purified to electrophoretic homogeneity, display the same affinity toward p-nitrophenyl-alpha-glucoside (Km = 0.24 mM at pH 4 and 70 degrees C), the same molecular weight on Sephadex G-200 (140,000-145,000) and isoelectric point (4.2) and are protected by Cl- and H+ ions from heat denaturation at 70 degrees C. They differ in the carbohydrate content which varies between 14 and 31%, w/w.
D001203:Ascomycota / Q000201:enzymology*; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D005959:Glucosidases / Q000302:isolation & purification*; D006358:Hot Temperature; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D000520:alpha-Glucosidases / Q000302:isolation & purification*
D002241:Carbohydrates; D005656:Fungal Proteins; D007527:Isoenzymes; D005959:Glucosidases; D000520:alpha-Glucosidases