title: A mitochondrial ribosomal RNA mutation and its nuclear suppressors.
abstract: We have isolated cold-resistant revertants from a mitochondrial cold-sensitive mutation, cs909, localized in the 21S ribosomal RNA gene. Two types of revertants have been isolated: (1) strong revertants which were shown to be due to a single, nuclear, dominant suppressor; (2) weak revertants which are all due to the presence of a single, nuclear, recessive suppressor. The recessive suppressor, when separated from the mitochondrial mutation, itself confers a cold-sensitive phenotype, that is, there is a mutual suppression between the mitochondrial cold-sensitive mutation and the nuclear cold-sensitive mutation. The suppressor by itself produces modified ribosomes and therefore probably codes for an element of the mitochondrial ribosome such as a ribosomal protein.
mesh_terms: D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal; D005800:Genes, Fungal; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D012335:RNA, Ribosomal / Q000235:genetics*; D012234:Rho Factor / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D013045:Species Specificity; D013489:Suppression, Genetic
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012234:Rho Factor; D004247:DNA