title: Endogenous mammalian lectin localized extracellularly in lung elastic fibers.
abstract: An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with colloidal gold conjugated second antibody. The endogenous mammalian lectin was found in smooth muscle cells and squamous alveolar epithelial (type I) cells and was concentrated extracellularly in elastic fibers of pulmonary parenchyma and blood vessels. The extracellular localization of this lectin suggests that it, like others, functions by interaction with extracellular glycoconjugates.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D002462:Cell Membrane / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005690:Galactose / Q000378:metabolism; D007106:Immune Sera; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification; D008168:Lung / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments / Q000032:analysis; D033902:Periplasmic Binding Proteins; D051381:Rats
chemical_list: D000936:Antigen-Antibody Complex; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D037102:Lectins; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D033902:Periplasmic Binding Proteins; C023550:galactose-binding protein; D005690:Galactose