An essential gene for replication of the mini-F plasmid from origin I.
We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Using these mutant mini-F plasmids, we found that F3 protein is essential for the replication of mini-F from origin I, but not from origin II.
D001426:Bacterial Proteins / Q000235:genetics; D004261:DNA Replication; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D010957:Plasmids
D001426:Bacterial Proteins; D004274:DNA, Recombinant