[Partial purification of the extracellular proteolytic system of Aeromonas hydrophila LP50: comparative chromatographic and electrophoretic study].
A complex extracellular proteolytic system was produced by Aeromonas hydrophila LP50 on glucose- polypeptone medium. Partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on Sephacryl- S200 and chromatography on DEAE-Sephacel. Every stage was controlled by electrophoresis. This proteolytic system was constituted of three aminopeptidase and two endopeptidase components.
D000333:Aeromonas / Q000201:enzymology*; D000626:Aminopeptidases / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification; D005110:Extracellular Space; D010447:Peptide Hydrolases / Q000302:isolation & purification*
D010450:Endopeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases
Purification partielle du système protéolytique extracellulaire de Aeromonas hydrophila LP50: étude comparative chromatographique et électrophorétique.