Selective inhibition of transition from sexual agglutination to zygote formation by ethyl N-phenylcarbamate in Saccharomyces cerevisiae.
Effects of ethyl N-phenylcarbamate (EPC) on the mating reaction of Saccharomyces cerevisiae were studied, with special attention on the effect on the alpha pheromone action. EPC inhibited zygote formation at a concentration which promoted induction of sexual agglutinability. EPC enhanced agglutinability induction by alpha pheromone, but inhibited alpha-pheromone-induced formation of large pearshaped cells in a mating type. The enhancement of agglutinability induction was accompanied with increased production of a agglutination substance and inhibition of alpha pheromone inactivation. EPC arrested the cell cycle of a cells probably in the step controlled by CDC19, CDC35, cAMP etc., just before the step controlled by CDC28, alpha pheromone etc.
D000371:Agglutination; D002219:Carbamates / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D000072235:Mating Factor; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D048448:Phenylcarbamates; D012098:Reproduction / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D014520:Urethane
D002219:Carbamates; D010455:Peptides; D048448:Phenylcarbamates; D014520:Urethane; D000072235:Mating Factor; C008866:phenylurethane