An immunohistological study of feline glomerulonephritis using the peroxidase-antiperoxidase method.
Twenty-two cases of feline glomerulonephritis were investigated for the presence of immune complexes within the glomerulus using the peroxidase-antiperoxidase (PAP) method. This method was used with formalin-fixed paraffin-wax embedded tissues which were pretreated with trypsin and with frozen sections of kidney tissue. Of a total of 25 kidney specimens examined (two cats had repeated biopsies) the composition of the deposits was 23/25 IgG, 17/25 C3, 11/25 IgM and 2/25 IgA. Serial studies of two cats showed a progression of the disease from initial nephrotic syndrome to chronic renal failure. With the more severe form of the disease there was a tendency for the deposition of complement and more than one class of immunoglobulin within the glomeruli.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D003176:Complement C3 / Q000032:analysis; D005921:Glomerulonephritis / Q000276:immunology / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007668:Kidney / Q000276:immunology*; D007676:Kidney Failure, Chronic / Q000276:immunology / Q000662:veterinary; D009404:Nephrotic Syndrome / Q000276:immunology / Q000662:veterinary
D000936:Antigen-Antibody Complex; D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M