Preparation of [B23-D-alanine]des-(B25-B30)-hexapeptide-insulin by a combination of enzymic and non-enzymic synthesis.
Des-(B25-B30)-hexapeptide-insulin with B23-glycine replaced by D-alanine was prepared by a combination of enzymic and non-enzymic syntheses. The purified product was homogeneous in polyacrylamide-gel electrophoresis and could be crystallized. The biological activity in vivo of crystalline [B23-D-Ala]des-(B25-B30)-hexapeptide-insulin was determined as 58% of that of standard pig insulin (27 i.u./mg).
D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000138:chemical synthesis*; D014357:Trypsin
D007328:Insulin; D010446:Peptide Fragments; C040194:insulin, des(hexapeptide)(B25-30)-Ala(B23)-; D014357:Trypsin