Production of an insulin-like growth factor by osteosarcoma.
To test the possibility that osteosarcoma cells produce their own growth factors, we measured levels of insulin and somatomedin C (SMC), an insulin-like growth factor, in culture media of two cell lines derived from patients with that disease. SMC but not insulin levels increased three- to ten-fold over a period of 7 days paralleling the increases in cell number. Production of SMC was inhibited by cycloheximide.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D013006:Growth Hormone / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000096:biosynthesis*; D007334:Insulin-Like Growth Factor I; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D010452:Peptide Biosynthesis; D013002:Somatomedins / Q000096:biosynthesis*
D003470:Culture Media; D007328:Insulin; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone