Bombesin-like immunoreactivity in the pancreas of man and other mammalian species.
Bombesin-like immunoreactivity has been measured in pancreatic tissues of man (12.4 +/- 1.2 pmol/g), pig (15.8 +/- 3.2), calf (4.3 +/- 0.9), rat (8.5 +/- 1.2) and guinea-pig (2.8 +/- 0.6) by a specific radioimmunoassay. Gel filtration of the pancreatic extracts revealed 2 major immunoreactive peaks: the earlier peak was eluted in the position of porcine gastrin-releasing peptide, and the later peak was eluted just after the amphibian bombesin standard. Immunocytochemistry demonstrated the presence of bombesin-like immunoreactivity in nerves in the rat pancreas, particularly in the exocrine pancreas, and occasionally in the peri-insular spaces. Isolated rat pancreatic islets were found to contain small quantities of bombesin-like immunoreactivity (0.037 +/- 0.003 fmol/islet) suggesting that mammalian bombesin-like peptides may be involved in the regulation of endocrine as well as exocrine pancreatic secretion.
D000818:Animals; D001839:Bombesin / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D010179:Pancreas / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013552:Swine; D014018:Tissue Distribution
D010455:Peptides; D001839:Bombesin