Cellular location of heat-labile enterotoxin in Escherichia coli.
We demonstrated that both the A and B subunits of heat-labile enterotoxin from Escherichia coli are located in the periplasm. The toxin was shown to form aggregates in Tris-EDTA buffers which are routinely used for isolating membranes. The aggregates pellet upon centrifugation, and this may explain why several previous investigators have concluded that enterotoxin is associated with membranes.
D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis*; D029968:Escherichia coli Proteins; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D009994:Osmolar Concentration
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; C027538:heat-labile enterotoxin, E coli