title: Reactivity of EMIT serum barbiturate assay reagents: potential application for the quantitation of selected barbiturates.
abstract: The reactivity of EMIT serum barbiturate assay reagents toward barbiturate test compounds was investigated. Reactivity was determined by the C5 substituents on the barbiturate ring and O/S substitution (barbituric/thiobarbituric acid derivatives). Reagents were more reactive toward a 5,5-substituted barbiturate than toward its hydroxylated analogue. EMIT reactivity could be linearized vs. concentration of test compound, allowing for quantitative analysis in serum or plasma.
mesh_terms: D001463:Barbiturates / Q000097:blood*; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics
chemical_list: D001463:Barbiturates