Polarization fluoroimmunoassay of biopterin and neopterin in human urine.
A polarization fluoroimmunoassay has been developed for the routine determination of biopterin and neopterin levels in human urine. The method employs fluorescein-labeled biopterin and neopterin. The assay is fast (incubation time: 2 min) and no separation step is required prior to measurement of fluorescence polarization. Linearity, recovery and precision were satisfactory. Estimations of biopterin and neopterin levels in human urine samples closely correlated with those obtained by radioimmunoassay.
D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives / Q000652:urine*; D005454:Fluorescence Polarization / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D019798:Neopterin; D011621:Pteridines / Q000652:urine*; D011817:Rabbits; D011863:Radioimmunoassay
D007106:Immune Sera; D011621:Pteridines; D001708:Biopterins; D019798:Neopterin