Stability of the hybrid plasmid pIM138 and its curing by some eliminating agents.
Hybrid plasmid pIM138 was constructed by insertion of a chromosomal fragment with the threonine operon from Escherichia coli into the pBR322 vector. Molar mass of pIM138 was 2.8 Mg/mol. Heteroduplexes between pBR322 vector and pIM138 hybrid DNA molecules were prepared. The hybrid plasmid shows a high stability against the curing effect of rifampicin and clorobiocin in E. coli SK1590 thr host.
D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D009675:Novobiocin / Q000031:analogs & derivatives / Q000494:pharmacology; D010957:Plasmids / Q000187:drug effects; D012293:Rifampin / Q000494:pharmacology
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D009675:Novobiocin; C006260:clorobiocin; D012293:Rifampin