Serotonin immunohistochemistry reveals immature EC cells.
Radioautographic studies of rat duodenal mucosa were carried out after single injections of 3H-thymidine, and the labelling indices of enterochromaffin (EC) cells identified by serotonin immunohistochemistry and by the diazo reaction were compared. The labelling index of EC cells by serotonin immunohistochemistry was 2.3% (mean percentage of 2 rats), while no labelled cells were found in diazo-positive EC cells. This finding indicates that serotonin immunohistochemistry reveals a less mature population of EC cells than conventional techniques such as the diazo reaction.
D000818:Animals; D001345:Autoradiography; D002838:Chromaffin System / Q000032:analysis*; D004386:Duodenum / Q000166:cytology; D004759:Enterochromaffin Cells / Q000032:analysis* / Q000166:cytology; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism
D012701:Serotonin; D013936:Thymidine