Immunohistochemical localization of apolipoprotein B in human atherosclerotic lesions.
A peroxidase-antiperoxidase immunohistochemical method was used to detect the presence of intracellular and extracellular apolipoprotein B (apo B) in paraffin sections of human abdominal aortas and coronary arteries with and without atherosclerotic involvement. The four aortas studied disclosed both intracellular and extracellular apo B in areas in which lipid was found by oil red O staining. Coronary arteries also demonstrated abundant extracellular apo B but failed to disclose intracellular apo B in cells with stainable lipid. Immunohistochemical detection of intracellular apo B in raised aortic lesions but not in cells of coronary lesions suggests that arteries that contain detectable amounts of intracellular apo B correspond to arteries that usually have earlier atherosclerotic involvement.
D000328:Adult; D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology; D001053:Apolipoproteins / Q000378:metabolism*; D001055:Apolipoproteins B; D001161:Arteriosclerosis / Q000473:pathology*; D003331:Coronary Vessels / Q000473:pathology; D005260:Female; D006041:Goats / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged
D001053:Apolipoproteins; D001055:Apolipoproteins B