Specificity of a human leukocyte neutral proteinase with bovine parathyroid hormone as substrate.
A neutral proteinase, located on the surface of human granulocytes and lymphocytes, degraded bovine parathyroid hormone in vitro. The observed sites of cleavage were between residues 5 and 6 (--ile--gln--), 8 and 9 (--met--his--), 38 and 39 (--gly--ala--) and 41 and 42 (--ile--ala--). Possible physiological implications of these findings are discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D015260:Neprilysin; D010281:Parathyroid Hormone / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D013379:Substrate Specificity
D000596:Amino Acids; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D010450:Endopeptidases; D015260:Neprilysin