Improving the Mycoplasma gallisepticum and M. synoviae antigen yield by readjusting the pH of the growth medium to the original alkaline state.
The pH of Mycoplasma gallisepticum (MG) and M. synoviae (MS) growth medium was readjusted back to the original alkaline state when the pH reached 6.1 (for MG) and 6.7 (for MS), and the medium was reincubated until the pH of the medium returned to 6.7 to 6.9. The result was that MG and MS antigen yields were 43 and 54% higher than yields obtained at the usual harvest time.
D000818:Animals; D000942:Antigens, Bacterial / Q000592:standards*; D001431:Bacteriological Techniques / Q000662:veterinary*; D002645:Chickens; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D009174:Mycoplasma / Q000145:classification / Q000254:growth & development / Q000276:immunology*; D012703:Serotyping / Q000662:veterinary; D014422:Turkeys
D000942:Antigens, Bacterial; D003470:Culture Media