title: Differential effects of calcium deprivation on the cytoskeleton of non-tumorigenic and tumorigenic rat liver cells in culture.
abstract: Normal rat liver T51B epithelial cells and Morris no. 7795 hepatoma cells growing exponentially were exposed for 24 h to standard medium containing low (0.02 mM) calcium, a concentration which drastically reduces the proliferation of normal but not tumour cells. Cell surface morphology was examined by scanning electron microscopy (SEM); and the distribution and organization of microtubules, cytokeratin and vimentin filaments, and microfilaments were analysed by indirect immunofluorescence microscopy using specific antibodies. Calcium deprivation caused the loss of intercellular cohesion in both cell types and the appearance of some microvilli and blebs, particularly on tumour cells. However, marked differential (normal vs tumour cells) effects on the organizational integrity of the cytoskeleton fibrillar network were observed. Extracellular calcium deprivation led to a particular rearrangement of microtubules, and a perinuclear accumulation of cytokeratin and vimentin filaments in normal, but not in tumour cells. A massive concentration of actin-containing microfilaments was observed in the cell periphery and blebs of hepatoma cells. In the light of the possible involvement of calcium in controlling cytoskeleton assembly, the differing cytoskeletal changes of the two cell types may be linked to their different proliferative capabilities in low-calcium medium.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D003599:Cytoskeleton / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000648:ultrastructure; D051381:Rats; D014746:Vimentin
chemical_list: D007381:Intermediate Filament Proteins; D014746:Vimentin; D002118:Calcium