The effect of phenylhydrazine on protein breakdown in rabbit reticulocytes.
In contrast to reticulocytes produced by bleeding reticulocytes produced by injections of phenylhydrazine exhibit a sizeable Antimycin A- and a large salicylhydroxamic acid (SHAM)-resistant proteolysis. In their cytosol there exist both ATP-independent and ATP-dependent protease systems. The substrate of proteolysis are denatured and aggregated proteins arising from reactions of phenylhydrazine with cell constituents. In phenylhydrazine-induced reticulocytes there is an overlap between the basic biological process of maturation-dependent proteolysis of mitochondria and the breakdown of denatured and aggregated "abnormal" proteins.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006470:Hemorrhage / Q000097:blood; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism; D010447:Peptide Hydrolases / Q000097:blood; D010659:Phenylhydrazines / Q000494:pharmacology*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects* / Q000378:metabolism; D012457:Salicylamides / Q000494:pharmacology
D001798:Blood Proteins; D010659:Phenylhydrazines; D012457:Salicylamides; C030299:phenylhydrazine; D000968:Antimycin A; D000255:Adenosine Triphosphate; C005703:salicylhydroxamic acid; D010447:Peptide Hydrolases