Affinity purification of aminoacyl-tRNA.
A procedure for separating Escherichia coli aminoacyl-tRNA from unacylated tRNA or components of the aminoacylation reaction, thereby achieving an aminoacyl-tRNA product with a very high specific activity, is described. The method utilizes the specific recognition of aminoacyl-tRNA for E. coli protein synthesis elongation factor Tu which has been immobilized on an affinity matrix. The application of the affinity procedure as a means of purifying a single aminoacyl-tRNA from an unfractionated mixture of tRNAs is also discussed.
D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity / Q000379:methods; D004926:Escherichia coli; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification*; D012117:Resins, Synthetic
D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl; D012117:Resins, Synthetic; D010444:Peptide Elongation Factor Tu