title: Influence of nutrient limitation and growth rate on the outer membrane proteins of Klebsiella aerogenes NCTC 418.
abstract: Four major proteins with molecular weights of 78 000, 37 000, 34 000 and 20 000 were present in the envelope of Klebsiella aerogenes when cultured at a high specific growth rate. However, at lower growth rates, the protein content and composition of the envelope depended on the imposed nutrient limitation. Under potassium-, carbon-, sulphur- and phosphorus-limited conditions, derepression of synthesis of limitation-specific proteins was observed, their apparent molecular weights being 90 000, 48 000, 41 000 and 36 000, respectively. Nitrogen-limited cells had no additional proteins. For a particular limiting nutrient, expression of the limitation-specific proteins was independent of the chemical or physical form in which the nutrient was supplied. Under potassium or sulphur limitation the specific proteins were present maximally at the lowest imposed growth rate, whereas under carbon limitation a maximum expression of these proteins was found at moderate growth rates. It is concluded that limitation-specific proteins which are associated with the outer membrane function in the uptake of limiting nutrients or, possibly, limitation-releasing compounds.
mesh_terms: D000641:Ammonia / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008970:Molecular Weight; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D013455:Sulfur / Q000494:pharmacology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003470:Culture Media; D010710:Phosphates; D013455:Sulfur; D000641:Ammonia; D005947:Glucose; D011188:Potassium