The control of morphogenesis in Candida albicans.
Morphogenesis (germ-tube formation) in Candida albicans was induced gratuitously by N-acetylhexosamine derivatives (N-acetyl-glucosamine covalently linked to agarose, N-acetylmannosamine, hyaluronic acid, colloidal chitin, and mucin). These compounds were not taken up by the yeast cells and did not support growth. 2-Deoxyglucose was a potent inhibitor of germ-tube formation (50 microM), but did not affect the yeast growth yield at a concentration of 2.5 mM. 2-Deoxyglucose covalently linked to agarose did not affect germ-tube formation, and the inhibition by free 2-deoxyglucose was overcome by the addition of glucose to the germ-tube-forming cells. Glucose competitively inhibited 2-deoxyglucose uptake (Ki = 0.14 mM), and these data indicate that 2-deoxyglucose acted intracellularly.
D000117:Acetylglucosamine / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D002686:Chitin / Q000494:pharmacology; D003470:Culture Media; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D006595:Hexosamines / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects; D009077:Mucins / Q000494:pharmacology; D013696:Temperature
D003470:Culture Media; D003837:Deoxy Sugars; D006595:Hexosamines; D009077:Mucins; D002686:Chitin; D006820:Hyaluronic Acid; D003847:Deoxyglucose; D000117:Acetylglucosamine; C002022:N-acetylmannosamine