A simple method for immunoselective cell separation with the avidin-biotin system.
A new method for immunoselective cell separation is described which uses biotinylated antibodies and avidin-coupled sheep erythrocytes for rosette formation, and a density gradient to separate the rosetting from the non-rosetting cells. The utility of the technique is illustrated by separating subpopulations of rabbit, mouse and rat lymphocytes. The method offers a rapid, sensitive and easily reproducible means for obtaining purified cell populations.
D000818:Animals; D001360:Avidin; D001666:Binding Sites, Antibody; D001710:Biotin; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012397:Rosette Formation
D001360:Avidin; D001710:Biotin