Antimonocyte serum. Preparation and characterization.
Cell suspensions enriched with monocytes were obtained from the blood of Vietnam normal pig and rabbits injected with avirulent Welshimer strain of L. monocytogenes by velocity sedimentation on BSA gradient and separation on Ficoll-Triosil gradient. They were used for immunization of rabbits and a sheep, respectively. The antisera specific for monocytes (RAMS and SAMS) were obtained by scrupulous absorption with lymphocytes and granulocytes.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D007158:Immunologic Techniques; D009000:Monocytes / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity; D013552:Swine
D007106:Immune Sera; C029764:antimacrophage globulin