[Diagnostic significance of LH-RH two step test in women with normoestrogenic amenorrhea: analysis of the self-priming effect of the anterior pituitary glands].
To investigate the self-priming effect of gonadotropin on the anterior pituitary glands, the response of serum gonadotropins to the "LH-RH two step test" (two step administration of 100 micrograms of synthetic LH-RH at a 60 minute interval) was studied in 20 women with normoestrogenic amenorrhea who were responsive to the progesterone test. The patients were divided into two groups according to the results of the clomiphene test. Blood samples were collected before LH-RH stimulation and 30, 60, 90, 120, 180 minutes after the first stimulation, respectively. The P1P2 ratio (2nd peak level/1st peak level) and delta 1 delta 2 ratio (delta 2/delta 1 = 2nd peak level-60 min. level/1st peak level-0 min. level) of LH and FSH in the clomiphene-positive group were significantly higher than those in the clomiphene-negative group. These ratios are regarded as the degree of the self-priming effect, and at least partially reflect the capacity for the synthesis of pituitary gonadotropins. The results demonstrate that the self-priming effect is more restored in the clomiphene-positive group than in the clomiphene-negative group, and the data also indicate that the analysis of the self-priming effect with the "LH-RH two step test" is useful in developing new diagnostic criteria on ovarian dysfunction.
D000328:Adult; D000568:Amenorrhea / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D002996:Clomiphene / Q000627:therapeutic use; D004958:Estradiol / Q000097:blood; D005260:Female; D007987:Gonadotropin-Releasing Hormone; D006065:Gonadotropins, Pituitary / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D010901:Pituitary Function Tests; D010903:Pituitary Gland, Anterior / Q000503:physiopathology*
D006065:Gonadotropins, Pituitary; D002996:Clomiphene; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol