title: A technique for recording from single neurons in the spinal cord of the awake cat.
abstract: A means of recording activity from single neurons in the spinal cord of the awake cat over periods of several weeks is described. The method combines elements of the chronically implanted well technique used in recording from supraspinal structures, with a novel method for reversibly stabilizing the vertebral column in a detachable 'outrigger' device. Between recording sessions, the animal is therefore freely mobile, its vertebral column again flexible. This preparation allows exploration of 1 cm2 of the spinal cord to all depths, and single neurons can be held for periods of 30 min to 2 h. The activity of primary afferents, intraspinal interneurons and motoneurons can be identified and their responses quantified during limb movement by combining ancillary techniques with the stabilization method.
mesh_terms: D000818:Animals; D002415:Cats; D005069:Evaluation Studies as Topic; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology; D009482:Neurophysiology / Q000379:methods*; D013116:Spinal Cord / Q000502:physiology*; D013238:Stereotaxic Techniques; D014851:Wakefulness