Visual detection of granulocyte surface antigens using the avidin-biotin complex.
A visual test for detection of granulocyte surface markers using the avidin-biotin complex (ABC) has been developed. That this assay is highly specific, reproducible, and sensitive was determined by studying the expression of HLA antigens on granulocytes with monoclonal antibodies. Further, using granulocyte specific alloantisera, the results of the ABC test compared well to data from leukoagglutination assays and indirect immunofluorescence tests. The assay is particularly advantageous in that granulocytes can be stored, only small amounts of cells and sera are needed, and heterogeneous cell populations can easily be studied.
D000372:Agglutination Tests; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008297:Male; D010047:Ovalbumin / Q000031:analogs & derivatives*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin