[Current methods of immunologic diagnosis in parasitology].
This review of the immunological diagnosis of parasitic diseases defines the various indications, the means of collection and preparation, the various levels of specificity and the choice of parasitic antigen which should be used for immuno-diagnosis. The detection and assay of circulating antibodies relies on the techniques of immuno-precipitation (immunodiffusion, immunoelectrophoresis, electrosyneresis), indirect agglutination (latex and haemagglutination) or the use of labelled compounds (immunofluorescence, enzymo-immunoassay, radio-immunoassay). Their respective advantages and disadvantages are discussed. The detection and assay of circulating antigens involve the use of agglutination techniques (mycoses), radio-immunoassay or enzymo-immunoassay (protozooses and helminthiases). The authors review the applications of immunological diagnosis for the helminthiases (Trichinosis, Toxocarosis, Filariasis, Anguillosis, Ascaridiasis, Echinococcosis, Taeniasis and Cysticercosis, Distomatosis and Schistosomiasis), the protozoan infections (malaria, Toxoplasmosis, Amebiasis, Trypanosomiasis, Leishmaniasis) and the mycoses (Aspergillosis, Candidiasis, Cryptococcosis). They also discuss the prospects for the development of immunological diagnosis by identification, purification and standardization of parasitic antigens and the study of circulating antigens and idiotypic anti-parasitic antibodies. Finally, they outline the respective responsibilities of the biologist and the prescribing doctor for the proper use of immunological diagnosis of parasitic diseases.
D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000276:immunology; D006373:Helminthiasis / Q000175:diagnosis; D006801:Humans; D009181:Mycoses / Q000175:diagnosis; D010271:Parasites / Q000276:immunology; D010272:Parasitic Diseases / Q000175:diagnosis* / Q000276:immunology; D011528:Protozoan Infections / Q000175:diagnosis; D012698:Serologic Tests / Q000379:methods
D000906:Antibodies; D000941:Antigens
Méthodes actuelles de diagnostic immunologique en parasitologie.