Insulin biosynthesis and secretion of isolated pancreatic islets of mice after the administration of anti-insulin serum of guinea pigs.
Comparative studies on the effect of intraperitoneal administration of 0.5 ml anti-insulin serum (AIS) of guinea pig or glucose (2 g/kg body wt) on biosynthesis and secretion of insulin were carried out in C 57 Bl/6 j mice. The islets of Langerhans isolated 1 hr after administration of AIS exhibited significantly increased biosynthesis and secretion of insulin during an incubation in presence of 5 mmol/l glucose. The result suggest that AIS may be a more potential stimulator of beta-cell function than glucose created by the decreased level of free insulin in early period of time following AIS administration.
D000818:Animals; D005947:Glucose / Q000494:pharmacology; D006168:Guinea Pigs; D007106:Immune Sera / Q000008:administration & dosage*; D007328:Insulin / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D007106:Immune Sera; D007328:Insulin; D005947:Glucose