The cell cycle and sorting behaviour in Dictyostelium discoideum.
Synchronized cells of the cellular slime mould Dictyostelium discoideum were prepared by mitotic wash-off. Cell counts and DNA synthesis measurements indicated a high degree of synchrony. Cells from each phase of the cell cycle were fluorescently labelled and mixed with unlabelled asynchronous cells. Cells that were in S-phase and very early G2 at the onset of starvation demonstrated a strong tendency to sort to the tip of the subsequent slugs. With reference to these results and published evidence, we discuss the possible role of cell-cycle-related adhesion differences in cell sorting.
D000268:Adhesiveness; D002453:Cell Cycle; D002454:Cell Differentiation; D002467:Cell Nucleus; D003584:Cytological Techniques; D004023:Dictyostelium / Q000166:cytology* / Q000378:metabolism; D007399:Interphase; D008938:Mitosis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D013936:Thymidine