What determines the degradation rate of an injected protein?
The fusion of cultured mammalian cells to red blood cells loaded with specific proteins provides a powerful system for the study of intracellular proteolysis. During the past four years the degradation rates of more than 30 proteins have been examined after their injection into HeLa cells. Results from these studies support the legitimacy of the microinjection approach. They also provide insight into the mechanism of intracellular proteolysis.
D000818:Animals; D000911:Antibodies, Monoclonal; D002459:Cell Fusion; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism; D006207:Half-Life; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008845:Microinjections; D010447:Peptide Hydrolases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011506:Proteins; D010447:Peptide Hydrolases