Vanillic acid, a metabolite of 4-hydroxy-3-methoxyphenylglycol and 4-hydroxy-3-methoxymandelic acid in man.
4-Hydroxy-3-methoxyphenylglycol (HMPG) labelled with 14C was used to study the metabolic fate of HMPG in six healthy volunteers. Besides conjugation and oxidation to 4-hydroxy-3-methoxymandelic acid (HMMA, VMA) a minor portion, 8.4 +/- 1.1% (mean +/- SEM) was excreted as 14C-labelled vanillic acid (VA). To study if VA was formed from HMPG or HMMA (VMA), deuterium-labelled HMPG [( 2H3]HMPG) and HMMA [( 2H6]HMMA) were simultaneously injected intravenously to seven healthy volunteers. The recovery of [2H3]VA from [2H3]HMPG was 8.3 +/- 2.1% and the recovery of [2H6]VA from [2H6]HMMA was 9.0 +/- 2.1%. The 2H-labelled VAs were probably formed by a decarboxylation reaction, in the case of HMPG after previous oxidation to HMMA.
D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer / Q000379:methods; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006018:Glycols / Q000378:metabolism*; D006801:Humans; D062385:Hydroxybenzoates / Q000378:metabolism*; D008734:Methoxyhydroxyphenylglycol / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D014641:Vanillic Acid / Q000378:metabolism*; D014642:Vanilmandelic Acid / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006018:Glycols; D062385:Hydroxybenzoates; D008734:Methoxyhydroxyphenylglycol; D014642:Vanilmandelic Acid; D003903:Deuterium; D014641:Vanillic Acid