Intraspecific characterization of T. cruzi stocks by the lectins from Triticum vulgaris and Arachis hypogaea.
The agglutination behavior of four-days-old epimastigote culture forms of T. cruzi, and T. cruzi-like stocks from different South American countries were tested with 15 lectins. Two agglutination types can be distinguished: Typ 1 includes stocks which react with Triticum vulgaris and Aaptos papillata II (WGA-type). Type 2 includes stocks agglutinated by Arachis hypogaea and Aaptos papillata II (PNA-type). The reactions with the lectins from Triticum vulgaris and Aaptos papillata II can be correlated with the presence of N-Acetylneuraminic acid on the cell surface of these flagellates. After treatment with neuraminidase the WGA-type T. cruzi-like Morcego 1354 is agglutinated by Arachis hypogaea but not by the N-Acetylneuraminic acid specific lectins from Triticum vulgaris and Aaptos papillata II.
D000372:Agglutination Tests; D000818:Animals; D010367:Arachis; D037102:Lectins / Q000276:immunology*; D037121:Plant Lectins; D013045:Species Specificity; D014908:Triticum; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000276:immunology*
D037102:Lectins; D037121:Plant Lectins