A modified azoalbumin technique for the assay of proteolytic enzymes for use in blood group serology.
A spectrophotometric assay of proteolytic enzyme activity is described in which the optical density obtained by a given enzyme preparation is related (2 alpha less than 0.01) to the serological activity of that preparation in a two stage technique. The assay appears to be of use for the standardisation of enzyme preparations for use in blood group serology.
D000418:Albumins; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D010447:Peptide Hydrolases / Q000097:blood*; D012015:Reference Standards; D013053:Spectrophotometry
D000418:Albumins; C031460:azoalbumin; D010447:Peptide Hydrolases