A DNA-binding protein from E. coli isolation, characterization and its relationship with proteins H1 and B1.
A protein of Mr 16,000 (protein 16 K) which tightly binds to DNA has been isolated from E. coli. The amino acid composition and amino-terminal amino acid sequence of this protein have been determined. On the basis of its physico-chemical characteristics the protein 16 K was shown to be identical to the protein H1 [Cukier-Kahn et al. (1972) Proc. Natl. Acad. Sci. USA 69, 3643-3647; Spassky and Buc (1977) Eur. J. Biochem. 81, 79-90] which corresponds itself to the protein B1 [Bakaev (1981) Molekulyarnaya Biologya 15, 1350-1362].
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity; D004247:DNA / Q000031:analogs & derivatives; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006657:Histones; D008970:Molecular Weight; D013329:Structure-Activity Relationship
D000596:Amino Acids; D004268:DNA-Binding Proteins; C033190:DNA-cellulose; D006657:Histones; D002482:Cellulose; D004247:DNA