title: Human bone marrow fibroblast colony-forming units (CFU-F).
abstract: The bone marrow population includes a cell type which is able to give rise in vitro to fibroblast colonies (CFU-F). CFU-F are adherent, nonphagocytic, nonproliferating, relatively radioresistant, Ia antigen-negative cells having a wide density and sedimentation rate distribution. CFU-F frequency in normal bone marrow (aspirates) is 68 +/- 10 (range: 40-143; n = 10), per 5 X 10(6) cells. The fibroblastic nature of CFU-F progeny is demonstrated by immunofluorescent staining with anti-type I and anti-type III collagen, which are components of the marrow intercellular space and known to be produced by fibroblasts.
mesh_terms: D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D003239:Connective Tissue Cells; D046508:Culture Techniques / Q000379:methods; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology / Q000528:radiation effects; D006801:Humans; D011836:Radiation Tolerance