[Evaluation of the immunoenzymatic method for the study of anti-Toxoplasma gondii type IgG antibodies in relation to the indirect immunofluorescence method].
The indirect immunofluorescence assay for detecting anti-Toxoplasma gondii serum IgG is compared with the enzyme immunoassay employing a commercial kit for IgG. Enzyme immunoassay test shows a high degree of sensitivity, but it is less specific in comparison with indirect immunofluorescence test. There is evidence of direct relation between values expressed in Optical Density and reciprocal dilutions of sera; moreover a direct relation appears between Optical Density values and International Units/cm3. The authors claim that is advisable to give enzyme immunoassay test results, not only as Optical Density values, but also as antibody dilutions and International Units/cm3.
D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D014122:Toxoplasma / Q000276:immunology*
D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic
Valutazione del metodo immunoenzimatico per la ricerca degli anticorpi di tipo IgG anti-"Toxoplasma gondii" in rapporto al metodo di immunofluorescenza indiretta.