Influence of Tris (hydroxymethyl) aminomethane on plasma insulin and glucose concentration of normal and streptozotocin-diabetic rats.
The influence of Tris buffer on plasma glucose and insulin concentrations was investigated in pentobarbital-anesthetized (60 mg/kg i.p.) Wistar rats. A bolus injection of neutralized Tris (5 mmol/kg; pH 7.4) caused a transient increase of plasma insulin concentration (+ 130 +/- 20 microU/ml; means +/- S means, n = 6) but did not change the glucose concentration. A continuous infusion of Tris (0.5 mmol/kg X min for 90 min) reduced the plasma glucose concentration from 8.7 +/- 0.42 to 5.1 +/- 0.33 mmol/l after 30 min. The plasma insulin concentration was elevated during the first 20 min (maximum +122 +/- 21 microU/ml after 10 min). In streptozotocin-diabetic rats (75 mg/kg i.v., 48 h prior to the experiments) an infusion of Tris changed neither glucose nor insulin concentration in plasma. The results indicate that in the rat Tris-induced hypoglycemia is always associated with a transient stimulation of insulin secretion.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D001794:Blood Pressure / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D004285:Dogs; D006339:Heart Rate / Q000187:drug effects; D007003:Hypoglycemia / Q000139:chemically induced; D007328:Insulin / Q000097:blood*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014325:Tromethamine / Q000494:pharmacology*
D001786:Blood Glucose; D007328:Insulin; D014325:Tromethamine