Energetics of active sodium-potassium transport following stimulation with insulin, adrenaline or salbutamol in rat soleus muscle.
The total metabolic energy expenditure associated with active Na-K-transport over the first 20 min of stimulation with insulin, adrenaline or salbutamol (delta HmNa-K) was determined from direct calorimetric and tracer ion flux measurements in isolated muscles at rest. The reversible work performed by the Na-K-pump during the same interval of time (WrevNa-K- was calculated as the product of the ouabain-suppressible Na-K transfers and the mean free energy increase imparted to the two ions as they are transported against their electrochemical gradients across the plasma membrane. Comparison of membrane potential and intracellular Na and K concentrations before and after the stimulation indicated that part of WrevNa-K had contributed to increase in ion electrochemical gradients in the preparation (i.e. had not been lost as heat) during the 20 min period. Accordingly, the maximum value of delta HmNa-K was taken as the sum of the ouabain-suppressible heat production and WrevNa-K. Following stimulation with insulin, adrenaline or salbutamol this maximum corresponded to 10, 10 and 12% respectively, of basal metabolism. Under the same three conditions, the minimum "energetic efficiency" of the active Na-K-transport process, defined as the ratio between WrevNa-K and maximum delta HmNa-K, was 35, 41 and 38%, respectively.
D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active; D004734:Energy Metabolism; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D013268:Stimulation, Chemical
D007328:Insulin; D010042:Ouabain; D012964:Sodium; D000420:Albuterol; D011188:Potassium; D004837:Epinephrine