Regulation of acetylcholinesterase activity by retinoic acid in a human neuroblastoma cell line.
The ability of retinoic acid (RA) to modulate acetylcholinesterase (AChE) activity in a human neuroblastoma cell line (LN-N-5) was examined. The specific activity of AChE was significantly increased 3 days after exposure of LA-N-5 to RA and reached its maximum values after 9 or more days of culturing. Dose-response experiments demonstrated that large increases of AChE occurred at RA concentrations between 10(-7) and 10(-6) M with maximum AChE values detected at 10(-6)-10(-5) M. Increased AChE activity paralleled neurite outgrowth in LA-N-5 cultures. These findings demonstrate that RA can regulate specific AChE activity in human neuroblastoma cells in a manner consistent with neuronal maturation.
D000110:Acetylcholinesterase / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D009447:Neuroblastoma / Q000201:enzymology* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D000110:Acetylcholinesterase