title: Echinococcus multilocularis: identification of proteins inducing antibody formation in metacestode infection.
abstract: An approach to the identification of parasite proteins which are immunogenic in natural infections is described, using the infection with the larval stage of Echinococcus multilocularis as a parasite model. Metacestode proteins were separated by SDS-polyacrylamide gel electrophoresis, and transferred electrophoretically to nitrocellulose sheets (Western blotting). Subsequently, immune recognition of the proteins was performed with various host sera and antigen-antibody complexes were detected enzymatically. Using homologous antisera, different patterns of immunogenic bands were revealed by sera of different host species. Cross-reactions with sera from individuals infected with unrelated helminths were analysed. Four proteins of E. multilocularis which failed to show any cross-reaction were identified.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D003411:Arvicolinae; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000032:analysis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D011506:Proteins / Q000032:analysis / Q000276:immunology*
chemical_list: D011506:Proteins