Influence of the membrane on T-2 toxin toxicity in Saccharomyces spp.
In growing cells of Saccharomyces cerevisiae and Saccharomyces carlsbergensis, T-2 toxin inhibits cell growth. We have examined the role of the yeast membranes in the uptake mechanism(s) of T-2 toxin. The effects of membrane-modulating agents, ethanol, cetyltrimethylammonium bromide, Triton X-100, and heat were studied; these agents were found to increase the sensitivity of the yeasts toward T-2 toxin. In the presence of 5% (vol/vol) ethanol, 2 micrograms of T-2 toxin per ml caused complete inhibition of growth. In the presence of 1 microgram of cetyltrimethylammonium bromide per ml, yeast cells became sensitive to T-2 toxin, starting with a concentration of 0.5 micrograms/ml. Triton X-100 at concentrations below 1% (vol/vol) sensitized the cells toward T-2 toxin, but at higher concentrations it protected the cells from T-2 toxin. Temperatures of incubation between 7 and 30 degrees C influenced the growth reduction caused by T-2 toxin. The greatest observed reduction of growth in T-2 toxin-treated cultures occurred at 30 degrees C. To further prove that the membrane influences the interaction of T-2 toxin with yeasts, we have studied a yeast mutant with a reduced plasma membrane permeability (G. H. Rank et al., Mol. Gen. Genet. 152:13-18, 1977). This yeast mutant proved to be resistant to T-2 toxin concentrations of up to 50 micrograms/ml. These results show that the membrane plays a significant role in the interaction of T-2 toxin with yeast cells.
D002462:Cell Membrane / Q000187:drug effects; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000633:toxicity; D003902:Detergents / Q000633:toxicity; D007700:Kinetics; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000633:toxicity; D012440:Saccharomyces / Q000187:drug effects* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000633:toxicity*
D002593:Cetrimonium Compounds; D003902:Detergents; D012717:Sesquiterpenes; D011092:Polyethylene Glycols; D017830:Octoxynol; D013605:T-2 Toxin; D000077286:Cetrimonium