Enzyme-linked immunosorbent assay (ELISA) for streptokinase antibodies.
A micro plate Enzyme-linked immunosorbent assay (ELISA), developed for detection of antibodies to Streptokinase, was used to analyze 80 human sera. The aim was to provide a simple method for antibody screening that required neither sophisticated equipment nor a high degree of technological skill. Preliminary results show that ELISA is specific, reproducible and sensitive. Comparative evaluation of the ELISA and the coagulum lysis test for antibodies against streptokinase show excellent correlation. We also evaluated the anti-Streptolysin O titer either by conventional method or ELISA. The sensitivity of ELISA for detecting anti-Streptolysin O antibodies was 3-4-fold higher than that of the hemolytic test. The sensitivity of ELISA for detecting anti-streptokinase antibodies was 10-20-fold higher than that of the coagulum lysis. The Enzyme-linked immunosorbent assay seems to offer some advantages over the more commonly used coagulum lysis test as far as sensitivity is concerned and potentially it is suggested that this method could be usefully employed clinically in human streptococcal infections.
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins; D001433:Bacteriolysis; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis; D006801:Humans; D007124:Immunoenzyme Techniques; D013300:Streptokinase / Q000276:immunology*; D013301:Streptolysins / Q000037:antagonists & inhibitors
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O; D013300:Streptokinase