Further purification of neutrophil migration inhibition factor from T lymphocytes (NIF-T): evidence that NIF-T and leukocyte inhibitory factor (LIF) are immunologically distinct.
Neutrophil migration inhibition factor from T lymphocytes (NIF-T) purified by antibody affinity chromatography and gel filtration chromatography was radioiodinated and identified as a 26,000-MW protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). NIF-T was identified by elution of biological activity from gel fractions and selective adsorption of a radioiodinated mediator by HL60 cells differentiated in the presence of dimethyl sulfoxide (DMSO) to develop receptors for NIF-T. A goat neutralizing antibody for NIF-T neutralized and immunoprecipitated migration inhibition activity in the conditioned medium from Mo T-lymphoblast cells and human peripheral blood lymphocytes (PBL) cultured with concanavalin A (Con A), but not from RPMI 1788 B-lymphoblast cells with leukocyte migration inhibitory factor (LIF) activity. These studies distinguish NIF-T both chemically and immunologically from LIF.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006041:Goats; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D007961:Leukocyte Migration-Inhibitory Factors / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology* / Q000302:isolation & purification*; D009500:Neutralization Tests; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000276:immunology*
D007074:Immunoglobulin G; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; C033370:neutrophil migration inhibitory factor; D004121:Dimethyl Sulfoxide