An avidin-biotin ELISA for the measurement of serum and secretory IgD.
This paper describes an improved microtiter solid-phase enzyme immunoassay for the determination of serum and secretory IgD. Use of the interaction between biotinylated anti-human IgD and horseradish peroxidase(HRP)-avidin conjugate permits quantitation of human IgD in the range of 1-64 ng/ml. IgD was detected in all samples of serum, saliva and nasal secretions of 28 normal adults. In only one subject both serum and secretory IgD were undetectable. The mean concentration of serum IgD determined by this assay is similar to that reported by other authors using radioimmunoassay. The assay described is not only rapid and inexpensive but at least as sensitive as the radioimmunoassays usually employed for quantitation of IgD.
D000328:Adult; D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007072:Immunoglobulin D / Q000032:analysis*; D009297:Nasal Mucosa / Q000378:metabolism; D012463:Saliva / Q000032:analysis
D007072:Immunoglobulin D; D001360:Avidin; D001710:Biotin