Kinetic-dependent enzyme-linked immunosorbent assay for detection of antibodies to Legionella pneumophila.
A semiautomated, kinetic-dependent, enzyme-linked immunosorbent assay (K-ELISA) was adapted to detect serum antibodies to Legionella pneumophila. In a comparative study, 158 human serum samples (79 pairs) were tested by K-ELISA and the standard indirect immunofluorescence assay for determination of antibody levels to L. pneumophila serogroup 1. K-ELISA determinations were made by using a serogroup-specific antigen or a preparation (unfractionated antigen) which contained both common antigen and serogroup-specific reactivity. There was good correlation between the immunofluorescence assay and the K-ELISA by using either antigen, although greater correlation was achieved with the unfractionated antigen (coefficients of correlation, 0.894 with unfractionated antigen and 0.841 with serogroup-specific antigen). These results indicate that the K-ELISA is a reliable alternative to the immunofluorescence assay for serologically diagnosing legionellosis.
D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007700:Kinetics; D007875:Legionella / Q000145:classification / Q000276:immunology*; D007877:Legionnaires' Disease / Q000175:diagnosis*; D012703:Serotyping
D000907:Antibodies, Bacterial