[Flow cytometric analysis of hepatocellular regeneration after partial hepatectomy and administration of hepatotrophic factors].
Kinetics of hepatocellular regeneration after 30% and 70% hepatectomy and the effect of hepatotrophic factors on the normal liver and regenerating livers after hepatectomy were investigated by flow-cytometric analysis using adult male rats. The results were as follows: The DNA histogram of hepatocytes in the normal group had two peaks in G0G1 and G2M components; G0G1 29% S 13%, G2M 58%. In the 30% hepatectomized group, the peak of G2M slightly increased, and remarkable change was not noticed during 4 weeks. In the 70% hepatectomized group, the percentage of hepatocytes in G0G1 and G2M were markedly changed during 2 days after hepatectomy. The peak of G0G1 moved to S after 24 hours, to G2M after 36 hours and returned to G0G1 after 48 hours. Afterwards the G0G1 gradually decreased while the G2M increased and they finally reached a single peak of G2M at the 7th day. Supernatant of the intestinal homogenate after hepatectomy and EGF initiated the cell kinetics in normal and regenerating rat livers. Changes on cell kinetics was not observed after insulin and glucagon administration.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005434:Flow Cytometry; D005934:Glucagon / Q000494:pharmacology*; D006498:Hepatectomy; D007328:Insulin / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D004815:Epidermal Growth Factor; D004247:DNA; D005934:Glucagon