The enhancing and sensitizing effects of donor blood components, including dendritic cells, in a rat renal allograft model.
In the DA-to-Lewis renal allograft model, donor whole blood enhanced renal allograft survival (14.5 +/- 7.6 days versus 6.9 +/- 0.6 days in controls [P less than 0.01]). The effect of individual cell components given in numbers equivalent to those present in the enhancing volumes of donor whole blood was studied. Immunization with donor red cells alone produced greater enhancement than that produced by whole blood (36.14 +/- 19.5 days [P less than 0.01]). B lymphocytes also enhanced allograft survival (16.0 +/- 3.9 days [P less than 0.01]). Although slight enhancement was observed with platelets (8.5 +/- 0.6 days) and 10(5) dendritic cells (8.4 +/- 0.5 days), in terms of allograft function dendritic cell immunization produced evidence of dose-dependent accelerated rejection. A similar finding was obtained with donor T cell immunization. Donor plasma had no effect. We conclude that, although donor blood has an overall enhancing effect on renal allograft survival in this model, the sensitizing and enhancing effects can be ascribed to individual types of cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000096:biosynthesis; D001803:Blood Transfusion / Q000379:methods; D001806:Blood Urea Nitrogen; D017707:Erythrocyte Transfusion; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D007114:Immunization; D016030:Kidney Transplantation; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000145:classification; D008297:Male; D008954:Models, Biological; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000637:transplantation
D000961:Antilymphocyte Serum