Immunochemical investigation of the interaction of egg-yolk lipoproteins with bovine spermatozoa.
Antiserum was raised in rabbits against an egg-yolk lipoprotein fraction previously shown to have cryoprotective properties. It was used in conjunction with alkaline phosphatase-conjugated goat anti-rabbit IgG to demonstrate lipoprotein interaction with bovine spermatozoa. The lipoprotein bound firmly to spermatozoa and was not removed by extensive washing, suggesting that the lipoprotein had become irreversibly associated with the sperm membranes.
D000818:Animals; D002417:Cattle; D003451:Cryoprotective Agents / Q000378:metabolism*; D004530:Egg Yolk / Q000032:analysis*; D005260:Female; D007124:Immunoenzyme Techniques; D008074:Lipoproteins / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D013094:Spermatozoa / Q000378:metabolism*
D003451:Cryoprotective Agents; D008074:Lipoproteins