Different forms of p53 detected by monoclonal antibodies in non-dividing and dividing lymphocytes.
The commitment of non-dividing cells (in G0) to enter division can be studied using primary cultures of lymphocytes. The cells are stable in G0 unless stimulated by mitogen such as concanavalin A (Con A). Commitment to enter the division cycle depends on the expression of gene(s) induced by Con A and the synthesis of p53 protein correlates with this commitment step. I show here that in unstimulated cells, a second form of p53 is synthesized and is restricted to G0.
D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*; D016159:Tumor Suppressor Protein p53
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D003208:Concanavalin A