title: Activation of trehalase by membrane-depolarizing agents in yeast vegetative cells and ascospores.
abstract: The membrane-depolarizing agents 2,4-dinitrophenol, carbonylcyanide m-chlorophenylhydrazone, and nystatin are known to cause a rapid increase in the cyclic AMP level in fungal cells. Addition of these proton ionophores to yeast stationary-phase cells or ascospores causes an immediate 10-fold increase in trehalase activity. This observation is in agreement with a role for cyclic AMP-induced phosphorylation in the activation process of trehalase. It also provides an explanation for previous results on the induction of trehalose breakdown by 2,4-dinitrophenol in resting yeast cells.
mesh_terms: D019297:2,4-Dinitrophenol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D004140:Dinitrophenols / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005658:Fungi / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology*; D009761:Nystatin / Q000494:pharmacology*; D010800:Phycomyces / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012966:Sodium Cyanide / Q000494:pharmacology; D013172:Spores, Fungal / Q000201:enzymology; D014198:Trehalase / Q000378:metabolism*
chemical_list: D004140:Dinitrophenols; D009570:Nitriles; D009761:Nystatin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D014198:Trehalase; D005947:Glucose; D012966:Sodium Cyanide; D019297:2,4-Dinitrophenol