Sensitive enzyme immunoassay for the detection of delta antigen and anti-delta, using serum as the delta antigen source.
A sensitive enzyme-linked immunosorbent assay (ELISA, EIA) was developed for the detection of delta antigen in serum treated with Tween 20. The serum delta antigen so derived was used in an ELISA for anti-delta. Both tests were specific and more sensitive than radioimmunoassay (RIA) when applied to testing parenteral drug abusers. It is concluded that the different sources of delta-antigen used may account for the different sensitivities noted, and that delta antigenaemia in acute infection may be more frequently detectable than was first thought, amounting to 71% of those with delta infection in this study and that these sera are a convenient alternative source of antigen.
D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000276:immunology; D006511:Hepatitis B Antigens / Q000032:analysis*; D035923:Hepatitis delta Antigens; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D019966:Substance-Related Disorders
D000914:Antibodies, Viral; D006511:Hepatitis B Antigens; D035923:Hepatitis delta Antigens