Non-random chromosome segregation in Neocurtilla hexadactyla is controlled by chromosomal spindle fibres: an ultraviolet microbeam analysis.
Single spindle fibres of Neocurtilla spermatocytes were irradiated by means of an ultraviolet microbeam. Irradiations were with monochromatic ultraviolet light. The single sex chromosome (the X1 univalent) reoriented after irradiation of its spindle fibre or of any of the spindle fibres associated with the heteromorphic bivalent (the X2Y bivalent): the X1 moved toward the Y half-spindle, and sometimes rotated as it moved. Irradiations of autosomal spindle fibres did not induce X1 movements, and hence the induction of reorientation is specific to irradiation of the spindle fibres associated with X1 or X2Y. In no case did the X2Y bivalent reorient; hence, the X1 is the active chromosome in ensuring that there is non-random segregation in Neocurtilla spermatocytes. The irradiations sometimes caused the X2Y bivalent to contrast, but the reorientation movements of the X1 were independent of the contraction of the X2Y bivalent. We suggest that the X1 and X2Y chromosomal spindle fibres form a network that is able to send signals to the X1 univalent to cause it to reorient.
D000818:Animals; D005260:Female; D008297:Male; D008858:Microscopy, Phase-Contrast; D009987:Orthoptera / Q000648:ultrastructure*; D013090:Spermatocytes / Q000528:radiation effects / Q000648:ultrastructure*; D008941:Spindle Apparatus / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014743:Videotape Recording; D014960:X Chromosome / Q000502:physiology* / Q000528:radiation effects; D014998:Y Chromosome / Q000502:physiology / Q000528:radiation effects