[Nuclear DNAase of the yeast Candida tropicalis].
Using stepwise extraction of chromatin from Candida tropicalis by NaCl (0.1-1.0 M) the protein dissociated by 0.3 and 0.6 M NaCl (fractions 0.3 and 0.6) possessing the DNAase activity were obtained. These DNAases are activated by Mg2+ and cause preferential hydrolysis of heat-denaturated DNA. Fraction 0.3 DNAase has a maximum at neutral values of pH (around 7.0) and causes endonucleolytic hydrolysis of DNA. Fraction 0.6 DNAase causes exonucleolytic hydrolysis of DNA but a maximum at alkaline pH (8.0). The properties of isolated chromatin DNAases of Candida tropicalis differ from those of the known DNAases of the yeast Saccharomyces cerevisiae.
D002175:Candida / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology*; D002843:Chromatin / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D008928:Mitochondria / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013917:Thrombin / Q000302:isolation & purification / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology
D002843:Chromatin; D003851:Deoxyribonucleases; D013917:Thrombin
DNKazy iader drozhzheĭ Candida tropicalis.