Participation of microtubules and microfilaments in the transcellular biliary secretion of immunoglobulin A in primary cultures of rat hepatocytes.
The biliary secretion of immunoglobulin A (IgA) in primary hepatocyte cultures was investigated by means of immunofluorescence. The characteristic accumulation of IgA in visible bile canaliculi was found to be strongly inhibited by vinblastine and colchicine or by cytochalasin B, but its surface binding was not.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008870:Microtubules / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014747:Vinblastine / Q000494:pharmacology
D007070:Immunoglobulin A; D003571:Cytochalasin B; D014747:Vinblastine; D003078:Colchicine