Separation of natural and antibody-dependent cytotoxicity by differential reactivity of human mononuclear cell Fc receptors with IgG.
Nylon wood non-adherent, human peripheral blood mononuclear cells which are reactive with the OKM1 monoclonal antibody could be separated into two subpopulations based on their Fc receptor reactivity with human monomeric IgG (FcR-IgG) using flow cytometry. The majority of natural killer cells was found primarily in the OKM1+ subset with low FcR reactivity. In contrast, effector cells for antibody-dependent cytotoxicity (K cells) were found in subsets with both high and low FcR-IgG reactivity.
D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D011961:Receptors, Fc / Q000378:metabolism*
D007074:Immunoglobulin G; D011961:Receptors, Fc