A new immunoperoxidase assay for Lolium perenne-specific IgE in serum based on the biotin/avidin system (BAS).
A new solid-phase immunoassay based on the biotin/avidin system (BAS) for measuring serum Lolium perenne (LP)-specific IgE antibody is described. LP-specific IgE was assayed by the BAS assay and RAST for comparison in the sera of thirty-two normal asymptomatic subjects RAST-negative for LP and of twenty-six subjects with hay fever and RAST-positive for LP. The specificity of the BAS assay for LP-specific IgE was demonstrated by absorption experiments. An overall agreement of 91% (53/58) was observed between the BAS and RAST and a high correlation (r = 0.87, P less than 0.001) was found between the LP-specific IgE determined by the two methods. The advantages of the BAS assay as compared to both the RAST and classical ELISA are discussed.
D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D001360:Avidin; D007073:Immunoglobulin E; D001710:Biotin