Molecular organization of the protein A gene and its expression in recombinant host organisms.
This report is a preliminary description of the cloning, DNA sequence determination, and expression of the protein A gene from Staphylococcus aureus in Escherichia coli and yeast recombinant host systems. The deduced amino acid sequences of the Fc-binding domains agree well with a previously published peptide analysis. The deduced amino acid sequence of the cell wall anchorage domain reveals 11 short repeating units (designated S repeats). The S repeat consensus sequence is the octapeptide Lys-Pro-Gly-Lys-glu-Asp-Gly-Asn. The potential for the use of genetic engineering to enhance the therapeutic utility of the protein A molecule is also discussed.
D000595:Amino Acid Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*
D004274:DNA, Recombinant; D013205:Staphylococcal Protein A