Immunocytochemical localizations of cytosolic and mitochondrial glutamic oxaloacetic transaminase isozymes in rat brain.
The localizations of cytosolic (s-) and mitochondrial (m-) glutamic oxaloacetic transaminase (GOT) were examined by immunocytochemical methods using specific antibodies. Staining of s-GOT-like immunoreactivity was seen in periglomerular cells of the olfactory bulb, and basket, stellate cells of the cerebellum, and second layer cells of the neocortex. On the other hand, m-GOT-like immunoreactivity was found in mitral cells and glomerular regions of the olfactory bulb and deep Golgi cells of the cerebellum. These different distributions of s- and m-GOT isozymes suggest that these isozymes are available as markers of glutamergic or aspartergic neurons.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D002531:Cerebellum / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D009412:Nerve Fibers / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D009830:Olfactory Bulb / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D001219:Aspartate Aminotransferases