Induction of unresponsiveness to skin allografts in adult mice disparate at defined regions of the H-2 complex. I. Effect of donor-specific bone marrow in ALS-treated mice.
The induction of unresponsiveness to skin allografts was studied in adult congenic B10 mice disparate either at isolated K, I, or D regions of the H-2 complex or at combined K/D and I regions. Recipient mice were treated with antilymphocyte serum (ALS) and injected with donor bone marrow after skin grafting. Unresponsiveness to antigens of the isolated K or D regions (class I) could not be induced, as shown by the inability of bone marrow to prolong graft survival in these mice compared with ALS-treated controls. Significant unresponsiveness to antigens of the isolated I region could be induced by ALS immunosuppression alone, and it was not possible to determine the additive effects of bone marrow. Unresponsiveness to antigens of the K or D regions, or both, could be induced when they were presented with antigens of the I region. This indicates that the I region exerts a tolerogenic effect on the K and D regions. The requirement for the concomitant presentation of antigens of the K/D and I regions to achieve unresponsiveness is consistent with our previous observations that active suppression is one of the mechanisms of the induction of unresponsiveness in ALS-treated, marrow injected mice.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology*; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D014181:Transplantation Immunology
D000961:Antilymphocyte Serum; D006183:H-2 Antigens