title: The Emmy Klieneberger-Nobel Award lecture. Reflections on recovery of some fastidious mollicutes with implications of the changing host patterns of these organisms.
abstract: Major advances have occurred the past few years in the cultivation of a number of new, fastidious mollicutes--events which can be traced directly to successful efforts to develop culture media for the expanding group of helical mollicutes (spiroplasmas) inhabiting plants and arthropods. A description of cultivation techniques successful in primary isolation of three unusual mollicutes, representing new mycoplasmas from man and animals and a new spiroplasma from ticks, emphasizes some important factors in recovery of wall-less prokaryotes with special cultural requirements. Vigorous efforts to understand the distribution of spiroplasmas in plant and insect hosts also led to the cultivation of new, non-helical mollicutes. Preliminary characterization of a number of these new agents offers strong evidence for a unique and distinct Acholeplasma and Mycoplasma flora of both plants and insects.
mesh_terms: D000127:Acholeplasma / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D001179:Arthropod Vectors / Q000382:microbiology; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D007313:Insecta / Q000382:microbiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000382:microbiology; D009174:Mycoplasma / Q000302:isolation & purification* / Q000502:physiology; D010935:Plant Diseases; D010944:Plants / Q000382:microbiology; D011247:Pregnancy; D013149:Spiroplasma / Q000302:isolation & purification* / Q000502:physiology; D013987:Ticks / Q000382:microbiology; D014526:Urethritis / Q000382:microbiology; D014566:Urogenital System / Q000382:microbiology
chemical_list: D003470:Culture Media