Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.
3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisphosphate is also a substrate for the ligation reaction with DNA restriction fragments with a 3'-terminate cytidylic acid residue. Oligo- and polynucleotides with a 3'-phosphorothioate group react readily with electrophiles as exemplified by the reaction with monobromobimane.
D001643:Bridged Bicyclo Compounds / Q000138:chemical synthesis*; D001952:Bridged-Ring Compounds / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D003845:Deoxycytosine Nucleotides / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics*; D013329:Structure-Activity Relationship; D013604:T-Phages / Q000201:enzymology*; D013873:Thionucleotides
C040790:3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate; C040789:3'-O-(5'-phosphoryldeoxycytidyl)phosphorothioate; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D003845:Deoxycytosine Nucleotides; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013873:Thionucleotides; D012343:RNA, Transfer; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)