Bioassay and radioimmunoassay of lactogens in sera from children.
Lactogen levels in sera from children have been determined using the Nb 2 lymphoma cell bioassay (BA) and conventional radioimmunoassay (RIA). Assays were done on samples obtained under basal conditions and after pituitary stimulation induced by insulin or arginine administration. There was a close correspondence between BA and RIA results (r = 0.94; n = 43). The average ratio of the BA and RIA estimates of the lactogen levels (BA/RIA) was 0.86 +/- 0.13 (mean +/- SD) and 0.82 +/- 0.17 for basal and stimulated conditions, respectively. The increased secretion of lactogens after pituitary stimulation was not found to be associated with a change in the BA/RIA ratio.
D000293:Adolescent; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001681:Biological Assay; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D006801:Humans; D007223:Infant; D007328:Insulin / Q000494:pharmacology; D008223:Lymphoma; D008297:Male; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D011388:Prolactin / Q000097:blood*; D011863:Radioimmunoassay; D051381:Rats
D007328:Insulin; D011388:Prolactin; D013006:Growth Hormone; D001120:Arginine