Preliminary X-ray crystallographic data on mouse submaxillary gland renin and renin-inhibitor complexes.
We have crystallized renin from the submaxillary gland of male mice, both in its native state, and in binary complex with transition-state analog inhibitors. The best of the many crystal forms examined consisted of tetragonal bipyramids with space group symmetry P41 21 2 (or its enantiomorph) and unit cell dimensions a = b = 91.4 A, c = 211.6 A; alpha = beta = gamma = 90 degrees. This tetragonal form was compatible with both inhibited and uninhibited renin. Two of the inhibitors used were synthesized as iodinated analogs; their binary complexes with renin may serve as single-site rational heavy atom derivatives. X-ray data beyond 2.8-A resolution have been collected by oscillation photography using the Cornell High Energy Synchrotron Source in Ithaca, NY.
D000595:Amino Acid Sequence; D000818:Animals; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012083:Renin / Q000302:isolation & purification / Q000378:metabolism*; D013363:Submandibular Gland / Q000201:enzymology; D014961:X-Ray Diffraction; D000092502:Renin Inhibitors
D009842:Oligopeptides; D012083:Renin; D000092502:Renin Inhibitors; C027509:renin inhibitory peptide