Application of a solid-phase immunofluorometric assay to the selection of monoclonal antibody specific for the adenovirus group-reactive hexon antigen.
An immunofluorometric assay (IFMA) has been evaluated as a screening assay to detect monoclonal antibodies to the group-specific antigen of the adenovirus hexon component. The antibodies were produced as mouse ascitic fluids from hybridoma cells generated from Balb/C mice immunized with purified adenovirus type 2 hexon component and crude adenovirus type 3 culture supernatants. The purified IgG fractions from all monoclonal ascitic fluids tested were identified as the IgG1 K mouse isotype. Antibody titers ranged from 102,400 to 204,800 by the IFMA, from 200 to 12,800 by indirect FA, and were generally nonreactive in counterelectrophoresis, complement fixation, hemagglutination-inhibition, serum neutralization, and immune electron microscopy titrations. The IFMA is a reliable method for quantitating low levels of specific antibody in large numbers of test samples, and is therefore ideal as a screening assay for monoclonal antibody in tissue culture fluids and in mouse ascitic fluids.
D000260:Adenoviruses, Human / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D001202:Ascitic Fluid / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D003168:Complement Fixation Tests; D003378:Counterimmunoelectrophoresis; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus