One-step purification of procolipase from human pancreatic juice by immobilized antibodies against human colipase86.
Purified antibodies to human colipase86 were coupled to CNBr-activated Sepharose 4B. The immunoadsorption column thus obtained was used to purify procolipase from human pancreatic juice in one step by immunoaffinity chromatography. A single form of procolipase was obtained, having similar biological properties as previously characterized procolipases from horse and pig. The sequence of the N-terminal propeptide was determined to be Ala-Pro-Gly-Pro-Arg. In bovine, equine and porcine procolipases the corresponding N-terminal sequence is Val-Pro-Asp-Pro-Arg.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003089:Colipases / Q000276:immunology / Q000302:isolation & purification*; D004792:Enzyme Precursors / Q000302:isolation & purification*; D006801:Humans; D007158:Immunologic Techniques; D010189:Pancreatic Juice / Q000201:enzymology*; D011506:Proteins / Q000302:isolation & purification*; D013552:Swine
D000596:Amino Acids; D003089:Colipases; D004792:Enzyme Precursors; D011506:Proteins