Separation and characterization of pyrimidine deoxyribonucleoside 2'-hydroxylase and thymine 7-hydroxylase from Aspergillus nidulans.
Partially purified preparations from Aspergillus nidulans were shown to catalyze two alpha-ketoglutarate dependent dioxygenase reactions: the pyrimidine deoxyribonucleoside 2'-hydroxylase (EC 1.14.11.3) and the thymine 7-hydroxylase (EC 1.14.11.6) reactions. These reactions showed an absolute requirement for alpha-ketoglutarate and molecular oxygen and were stimulated by Fe(II), ascorbate and catalase. Both reactions demonstrated a stoichiometry such that for each mole of substrate (deoxyribonucleoside or pyrimidine) hydroxylated one mole of CO2 was produced from alpha-ketoglutarate. These two activities were separated using DEAE-Sephacel chromatography.
D001233:Aspergillus nidulans / Q000201:enzymology*; D007656:Ketoglutaric Acids / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000302:isolation & purification* / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology
D007656:Ketoglutaric Acids; D006899:Mixed Function Oxygenases; C023162:thymidine,2-oxoglutarate dioxygenase; C023164:thymine, 2-oxoglutarate dioxygenase; D010100:Oxygen