Trypanosoma cruzi: quantification and analysis of the infectivity of cloned stocks.
The infection of bovine embryo skin and muscle cells by trypomastigotes of four Trypanosoma cruzi clones (CA-I/71, /72, Miranda/76, /80) was quantified. Stable and reproducible intra-isolate differences were observed; an almost 70-fold difference in infectivity occurred between clones. The CA-I/71 clone was not susceptible to N-acetyl-D-glucosamine at a concentration that inhibits the infection of vertebrate cells by Ernestina and Y-strain parasites. Eight other monosaccharides that are common constituents of vertebrate cell surface glycoproteins also failed to inhibit the infection of vertebrate cells by the CA-I/71 clone.
D000117:Acetylglucosamine / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002999:Clone Cells; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005944:Glucosamine / Q000031:analogs & derivatives*; D009005:Monosaccharides / Q000494:pharmacology*; D009132:Muscles / Q000469:parasitology*; D012867:Skin / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
D009005:Monosaccharides; D005944:Glucosamine; D000117:Acetylglucosamine