A novel method for determination of beta-lactamase using the agar dilution method.
A novel test procedure is described for the rapid assay of beta-lactamase using the agar dilution method. The susceptibility to beta-lactamase was measured by adding an appropriate amount of enzyme to the diluting culture broth of a test organism in the agar dilution method. By this method, beta-lactamase susceptibility can be expressed in the same way as the MIC assay for antibiotics (microgram/ml). The results obtained with Escherichia coli beta-lactamase were closely related to the MIC patterns of the strains producing beta-lactamases.
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000032:analysis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; D000362:Agar; D001618:beta-Lactamases