Spectrofluorometric determination of captopril plus captopril disulfide metabolites in plasma.
Captopril disulfides and the drug covalently bound to proteins were reduced with tri-n-butylphosphine. After sample purification on an XAD-2 column, captopril was treated with 1-(7-dimethylamino)-4-methyl-2-oxo-2H -1-benzopyran-3-yl)-1H-pyrrole-2,5-dione to form a fluorescent derivative. After acidification, the fluorescent derivative was extracted into toluene and purified on a C18 cartridge. The fluorescence of the dimethyl-formamide eluate was measured at an excitation wavelength of 380 nm and a fluorescence wavelength of 440 nm.
D001711:Biotransformation; D002216:Captopril / Q000031:analogs & derivatives* / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D011392:Proline / Q000031:analogs & derivatives*; D013050:Spectrometry, Fluorescence / Q000379:methods
D011392:Proline; D002216:Captopril; C035974:captopril disulfide