title: Fusobacterium necrophorum isolated from a hepatic abscess and from mastitic udder secretions in a heifer.
abstract: Strains of Fusobacterium necrophorum were isolated from secretions of an udder affected with mastitis (4 strains), an adjacent unaffected udder (3 strains), a superficial inguinal lymph node (2 strains) and a liver abscess (4 strains). These 13 strains were examined bacteriologically together with three known strains: F. necrophorum VPI-2891 and VPI-6161, and Sphaerophorus necrophorus (F. necrophorum) N-167. All strains produced butyric acid from glucose and propionic acid from lactic acid, were positive for the indole reaction, failed to hydrolyse esculin, caused beta-haemolysis and showed sensitivity to penicillin (500 U/ml). They were divided roughly into two groups on the basis of differences in their properties as described below. Six strains belonged to one group and ten strains to the other: the former agglutinated chick red blood cells, formed colonies of type A of Fiévez, were long rods, showed turbid growth in liquid medium and were highly pathogenic for mice; the latter strains, consisting of short bacilli and forming colonies of type B, had no or weak haemagglutinin, showed, in liquid medium, sedimentary growth with a clear supernatant and were not fatal to mice. The 4 strains isolated from the hepatic abscess as well as the F. necrophorum strain VPI-2891 and the Sphaerophorus necrophorus strain N-167 were classified into biovar A. The 9 strains isolated from udder and lymph node, as well as the F. necrophorum strain VPI-6161, were classified into biovar B.
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003470:Culture Media; D005260:Female; D005674:Fusobacterium Infections / Q000662:veterinary*; D005675:Fusobacterium necrophorum / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D008100:Liver Abscess / Q000382:microbiology / Q000662:veterinary*; D008198:Lymph Nodes / Q000382:microbiology; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology*
chemical_list: D003470:Culture Media