A serum-free defined medium for retinal pigment epithelial cells.
Human and bovine RPE cells underwent changes in morphology and culture doubling times when passaged in serum-supplemented medium (CM). Furthermore, late passage human RPE cells subcultured in CM medium increased synthesis of three acidic, 43 000-63 000 D proteins. In order to provide a controlled environment for the study of RPE cells in vitro, we have developed a method for growing human and bovine RPE in a serum-free defined medium (DM). RPE cells grown in DM required a 24 h pretreatment with CM to allow the cells to attach and spread on the substrate. Cells grown in DM retained an epithelioid morphology, a stable culture doubling time, and similar 2-D PAGE patterns through several subculturings.
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D014168:Transferrin / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D014168:Transferrin; D014212:Tretinoin; D004815:Epidermal Growth Factor