Inhibition of DNA-dependent RNA polymerase from Escherichia coli by pyran copolymer.
Pyran copolymer, a potent inhibitor of DNA-dependent RNA polymerase from Escherichia coli, prevented polyribonucleotide synthesis by blocking both the initiation and elongation steps. The inhibition was noncompetitive with respect to template and nucleotide triphosphate substrates. Template binding and the stability of the nascent RNA chain were not affected by the inhibitor.
D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D011067:Poly dA-dT / Q000378:metabolism; D011108:Polymers / Q000494:pharmacology*; D011131:Polyribonucleotides / Q000096:biosynthesis; D011713:Pyran Copolymer / Q000494:pharmacology*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D011108:Polymers; D011131:Polyribonucleotides; D011067:Poly dA-dT; D011713:Pyran Copolymer; D012321:DNA-Directed RNA Polymerases