title: [Properties of Escherichia coli alpha-hemolysin].
abstract: SH-reagents (alpha-ethyl maleimide, n-chlor mercuribenzoate, dithioglycolic acid) and hydrogen peroxide induce insignificant changes in the activity of hemolysin. Reducing reagents (2-mercaptoethanol and ascorbate) inhibit hemolytic activity. Cholesterol at a concentration of 4.3 X 10(-5) M reduces this activity by 50%. Hemolysin has no phospholipase A activity. The energy necessary for activating the interaction of hemolysin with the membranes of erythrocytes (lag period) is 13800 cal/mol and for inducing the lysis of erythrocytes, 10 600 cal/mol. The above values are much less than those of O-labile hemolysins.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli; D005260:Female; D006460:Hemolysin Proteins / Q000032:analysis / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008297:Male; D010740:Phospholipases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature
chemical_list: D006460:Hemolysin Proteins; D013439:Sulfhydryl Reagents; D010740:Phospholipases
vernacular_title: Nekotorye svoĭstva al'fa-gemolizina Escherichia coli.