Pentobarbital quantitation using EMIT serum barbiturate assay reagents: application to monitoring of high-dose pentobarbital therapy.
Pentobarbital serum concentrations associated with a high-dose therapeutic regimen were determined using EMIT immunoassay reagents. Replicate analyses of serum controls resulted in a within-assay coefficient of variation of 5.0% and a between-assay coefficient of variation of 10%. Regression analysis of 44 serum samples analyzed by this technique (y) and a reference procedure (x) were y = 0.98x + 3.6 (r = 0.98; x = ultraviolet spectroscopy) and y = 1.04x + 2.4 (r = 0.96; x = high-performance liquid chromatography). Clinical evaluation of the results indicates the immunoassay is sufficiently sensitive and selective for pentobarbital to allow accurate quantitation within the therapeutic range associated with high-dose therapy.
D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D010424:Pentobarbital / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D007202:Indicators and Reagents; C038670:3'-hydroxypentobarbital; D010424:Pentobarbital