Release of liposomal markers by Cerebratulus toxin A-III.
Marker release from liposomes induced by the cytolytic protein Cerebratulus lacteus toxin A-III was studied. No phospholipid specificity was apparent, but the sensitivity of liposomes to A-III varied with the membrane fluidity. With dioleylphosphatidylcholine liposomes, complete release occurred at 10-20 micrograms toxin per ml, depending on marker size. Kinetic experiments showed that release was rapid and exhibited no lag phase. The diameter of the A-III produced membrane lesion must exceed 90 A, as tetrameric Concanavalin A is quantitatively released from A-III treated liposomes.
D000818:Animals; D003208:Concanavalin A / Q000378:metabolism; D003603:Cytotoxins / Q000494:pharmacology*; D019286:Endopeptidase K; D010450:Endopeptidases / Q000378:metabolism; D007448:Invertebrates; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D008387:Marine Toxins / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
C026601:Cerebratulus lacteus toxin A-III; D003603:Cytotoxins; D008081:Liposomes; D008387:Marine Toxins; D008563:Membrane Lipids; D010743:Phospholipids; D003208:Concanavalin A; D013395:Sucrose; D012964:Sodium; D010450:Endopeptidases; D019286:Endopeptidase K