Dual properties of cultured retinoblastoma cells: immunohistochemical characterization of neuronal and glial markers.
The dual properties of two human retinoblastoma cell lines, WERI-Rb1 and Y79, were investigated with immunohistochemistry. Two neuron-specific markers, dopamine-B-hydroxylase (DBH) and tetanus toxin, and an astrocyte-specific marker, the glial fibrillary acid protein (GFAP), were applied for immunohistochemical reactions. With peroxidase-antiperoxidase (PAP) and immunofluorescence techniques, all of the WERI-Rb1 and Y79 cells showed consistently positive results with both neuronal and glial markers. The findings demonstrate that cultured retinoblastoma cells WERI-Rb1 and Y79 have both neuronal and glial properties.
D002460:Cell Line; D002478:Cells, Cultured; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D005134:Eye Neoplasms / Q000032:analysis* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D009457:Neuroglia / Q000032:analysis; D009474:Neurons / Q000032:analysis; D012175:Retinoblastoma / Q000032:analysis* / Q000473:pathology; D013744:Tetanus Toxin / Q000032:analysis*
D005904:Glial Fibrillary Acidic Protein; D013744:Tetanus Toxin; D004299:Dopamine beta-Hydroxylase