Ultrastructural localization of gastric inhibitory polypeptide (GIP) in a well characterized endocrine cell of canine duodenal mucosa.
The polypeptide hormone GIP has been localized ultrastructurally by using specific, monoclonal GIP antibodies and an immunogold technique on aldehyde-osmium fixed specimens of dog duodenal mucosa. A single type of cell showing round, homogeneous, fairly osmiophilic granules with closely applied membrane and a mean size of 188 nm +/- 34 SD has been identified as the GIP cell.
D000818:Animals; D000911:Antibodies, Monoclonal; D003594:Cytoplasmic Granules / Q000032:analysis* / Q000648:ultrastructure; D004285:Dogs; D004386:Duodenum / Q000032:analysis* / Q000648:ultrastructure; D005749:Gastric Inhibitory Polypeptide / Q000032:analysis* / Q000276:immunology; D005768:Gastrointestinal Hormones / Q000032:analysis*; D006046:Gold; D007158:Immunologic Techniques; D007413:Intestinal Mucosa / Q000032:analysis* / Q000648:ultrastructure; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D005768:Gastrointestinal Hormones; D013205:Staphylococcal Protein A; D005749:Gastric Inhibitory Polypeptide; D006046:Gold