Disorganization of microfilaments caused by tumor promoters in mouse fibroblasts.
The effect of tumor promoters on the organization of BALB/c 3T3 fibroblasts was examined by indirect immunofluorescence microscopy using anti-actin antibodies. Potent tumor promoters such as 12-O-tetradecanoylphorbol-13-acetate, teleocidin, dihydroteleocidin B and debromoaplysiatoxin , in spite of their different chemical structures, all induced disruption of microfilaments. This change was still observed in the presence of actinomycin D.
D000199:Actins / Q000032:analysis; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D051379:Mice; D008807:Mice, Inbred BALB C
D000199:Actins; D002273:Carcinogens; D003609:Dactinomycin