Ion-exchange chromatographic characterization of stinging insect vespid venoms.
Paper wasp, hornets and yellow jacket vespid venoms were analysed by electrofocusing-electrophoresis titration curves and cation exchange chromatography on Mono STM. Important protein components were identified by zymography. The elution profiles from the cation exchange column for the different vespid venoms differed significantly, demonstrating the use of this technique for identification purposes. The various fractions from the ion-exchange elution profiles were collected and analysed with respect to enzymatic activity. The high capacity of the system makes it also suitable for preparative scale separation.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001514:Bee Venoms / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D006821:Hyaluronoglucosaminidase / Q000032:analysis; D007525:Isoelectric Focusing; D010447:Peptide Hydrolases / Q000032:analysis; D010740:Phospholipases / Q000032:analysis; D014862:Wasp Venoms / Q000032:analysis*
D001514:Bee Venoms; D014862:Wasp Venoms; D010740:Phospholipases; D000135:Acid Phosphatase; D006821:Hyaluronoglucosaminidase; D010447:Peptide Hydrolases