title: Hemodynamic and hematologic changes in a standardized trauma-sepsis model in rats.
abstract: The aim of this report was to describe an experimental trauma-sepsis model in rats. Trauma consisted of laparotomy and sepsis was induced by intravenous infusion of different amounts (10(8)-10(10)) of live Escherichia coli. Three experimental series were performed. In the first series survival rates were studied up to 7 days after trauma-sepsis. In the second and third series pulse rate, intraarterial blood pressure, cardiac output, liver blood flow and hematologic changes were studied after 24 h. Cardiac output was determined by dye-dilution method and hepatic blood flow by 133Xe wash-out technique. Survival rates were decreased in a dose-related manner when the amount of bacteria administered was increased from 10(8) to 10(10). Twenty-four hours after trauma-sepsis, survival rate was 100% following E. coli 10(8), 69% following E. coli 10(9), and 23% following E. coli 10(10). After 7 days the corresponding figures were 85%, 31%, and 8%. A dose-related decrease was also observed for cardiac output and hepatic blood flow when the amount of bacteria was increased. Mean arterial blood pressure was unchanged. Platelet and white blood cell counts were reduced 24 h following trauma-sepsis, and these changes were also more pronounced when the amount of bacteria infused was increased. Thus, it was possible to demonstrate a graded response following increasing amounts of bacteria regarding survival rate, cardiac output, liver blood flow, and hematologic changes in three subsequent experimental series. A carefully standardized experimental model may be of value for studies of metabolic events following trauma and sepsis.
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002302:Cardiac Output; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000097:blood / Q000503:physiopathology; D006339:Heart Rate; D006439:Hemodynamics; D007813:Laparotomy / Q000009:adverse effects; D008102:Liver Circulation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013530:Surgical Wound Infection / Q000097:blood / Q000503:physiopathology*