Application of hybridoma technology to the diagnosis of parasitic disease.
Recent applications of hybridoma-derived monoclonal antibodies to the diagnosis of parasitic disease are reviewed. Diagnostic tests, utilizing monoclonal antibodies, have included radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques to detect parasite antigen in host tissues and body fluids and circulating host antiparasite antibody. In general, the use of monoclonal-derived reagents has greatly increased the specificity of diagnosis by eliminating cross-reactions between closely related parasite species, without suffering a significant loss of sensitivity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006373:Helminthiasis / Q000175:diagnosis*; D006374:Helminthiasis, Animal; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D011528:Protozoan Infections / Q000175:diagnosis*; D011529:Protozoan Infections, Animal; D011863:Radioimmunoassay; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014235:Trichinellosis / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal