Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus.
A solid-phase enzyme immunoassay for the quantitative determination of the bitter triterpene-lactone, limonin, in citrus juice samples is described. As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forced-air microplate incubator.
D000469:Alkaline Phosphatase; D001628:Beverages / Q000032:analysis; D002957:Citrus / Q000032:analysis*; D003429:Cross Reactions; D007124:Immunoenzyme Techniques; D036701:Limonins; D014315:Triterpenes / Q000302:isolation & purification*
D036701:Limonins; D014315:Triterpenes; D000469:Alkaline Phosphatase; C001546:limonin