[Determination of the mitotic index in tumors by using cell suspensions].
A new variant of the method for mitotic index determination is offered. For this, a tumor cell suspension is obtained by treating the tumor with a 0.3% trypsin solution. Then the cells are transferred to a hypotonic solution where these undergo lysis and, in result, the nuclei are seen to be released in the medium. The ratio of nuclei being in meta- and anaphase is determined. Determination of the mitotic index in tumors using this method allows to avoid a technical error associated with the fact that the proliferative activity is not uniform in different parts of the tumor.
D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques; D007038:Hypotonic Solutions; D008175:Lung Neoplasms / Q000473:pathology*; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D013535:Suspensions; D014357:Trypsin / Q000494:pharmacology
D007038:Hypotonic Solutions; D013535:Suspensions; D014357:Trypsin
Opredelenie mitoticheskogo indeksa v opukholiakh s ispol'zovaniem kletochnykh suspenziĭ.