A simple and inexpensive method for assessing in vitro candidacidal activity of leukocytes.
Candidacidal activity of mouse neutrophils and macrophages was determined directly in microtiter plates. After a suitable period of interaction between phagocytic cells and C. albicans in the wells, the mouse cells were lysed with distilled water and corn meal agar was added to each well. Following incubation at 37 degrees C, viability was assessed using an inverted microscope and counting the number of germ tubes or microcolonies which developed. This method does not use radioisotopes or vital stains and should be applicable to other genera of yeasts.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D002176:Candida albicans / Q000254:growth & development; D002177:Candidiasis / Q000276:immunology / Q000382:microbiology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008828:Microbiological Techniques; D009504:Neutrophils / Q000502:physiology*