title: Lectin receptors as markers of lymphoid cells. I. Demonstration in tissue section by peroxidase technique.
abstract: The distribution of lectin binding receptors in human lymphoid tissue (lymph node and tonsil) was studied in an effort to identify lectins with potential application as cell markers. Using a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, the binding properties of five lectins were investigated: concanavalin A (Con A), Arachis hypogaea (peanut agglutinin), Lotus tetragonolobus (asparagus pea), Glycine max (soybean agglutinin) and Bandeiraea simplicifolia. Three of the lectins were found to demonstrate selective binding in tissue section: Lotus tetragonolobus to transformed follicular center lymphocytes; Arachis hypogaea to transformed follicular center lymphocytes; and Con A to macrophage histiocytes. These lectins are potentially useful markers for the investigation and classification of lymphoproliferative disease.
mesh_terms: D010367:Arachis; D003208:Concanavalin A / Q000378:metabolism; D006644:Histiocytes / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008221:Lymphoid Tissue / Q000378:metabolism*; D014066:Palatine Tonsil / Q000378:metabolism; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis*; D030262:Soybean Proteins; D013025:Glycine max; D010835:Phytohemagglutinins
chemical_list: D003208:Concanavalin A; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen; D030262:Soybean Proteins; C045475:Griffonia simplicifolia lectins; C036292:pea lectin; C028824:soybean lectin; D010835:Phytohemagglutinins