Initial reactions involved in the dissimilation of mandelate by Rhodotorula graminis.
Rhodotorula graminis utilized DL-mandelate, L(+)-mandelate, and D(-)-mandelate as sole sources of carbon and energy. Growth on these aromatic substrates resulted in the induction of an NAD-dependent D(-)-mandelate dehydrogenase and a dye-linked L(+)-mandelate dehydrogenase, each catalyzing the stereospecific conversion of its respective enantiomer of mandelate to benzoylformate. Benzoylformate was oxidized to benzaldehyde, which was dehydrogenated to benzoate by an NAD-dependent benzaldehyde dehydrogenase. Benzoate was further metabolized through p-hydroxybenzoate and the protocatechuate branch of the beta-ketoadipate pathway.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D007700:Kinetics; D008333:Mandelic Acids / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000378:metabolism*; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
D008333:Mandelic Acids; D000429:Alcohol Oxidoreductases; C043630:D-mandelate dehydrogenase; C021538:L-mandelate dehydrogenase; C037938:mandelic acid