Binding properties of mechanically and enzymatically isolated hepatocytes for IgG and C3.
The presence of Fc and C3 receptors was studied on mechanically and enzymatically isolated rabbit, mouse and rat hepatocytes as well as on hepatocytes derived from primary cultures. The same cell preparations were used for studying the uptake of an antibody against nuclear antigens. Mechanically isolated hepatocytes were able to bind AIgG and activate complement. However, the same cells were not able to form rosettes with EA or with EAC. Enzymatically isolated hepatocytes did not bind AIgG or activate complement nor did they form rosettes with EA or with EAC. The mechanically isolated cells and the nonviable fraction of the enzymatically isolated cells showed a nuclear fluorescence when incubated with an antibody against nuclear antigens. We conclude that enzymatically isolated hepatocytes lack Fc and C3 binding sites. Furthermore, the damage to the plasma membrane of the non-viable cells allows the penetration of antibodies against intracellular antigens.
D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003176:Complement C3 / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D011951:Receptors, Complement / Q000032:analysis*; D011961:Receptors, Fc / Q000032:analysis*
D003176:Complement C3; D007074:Immunoglobulin G; D011951:Receptors, Complement; D011961:Receptors, Fc