Beta 2 microglobulin-like polypeptide of the goldfish, Carassius auratus.
Antisera to human beta 2 microglobulin (beta 2M) detected a plasma membrane molecule on goldfish (Carassius auratus) cells in immunofluorescence. A goldfish molecule detected by radioimmunoassay (RIA) co-eluted with human beta 2M on gel filtration. By affinity chromatography on immobilized antibody to human beta 2M, a molecule was purified (from extracts of goldfish) that showed, on SDS-polyacrylamide gel electrophoresis, a mobility similar to that of human beta 2M (apparent Mr 12,800 +/- 500).
D000818:Animals; D002850:Chromatography, Gel; D003429:Cross Reactions; D003530:Cyprinidae / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006054:Goldfish / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D011863:Radioimmunoassay; D001613:beta 2-Microglobulin / Q000276:immunology / Q000302:isolation & purification*
D008565:Membrane Proteins; D001613:beta 2-Microglobulin