Mode of action of a bactericidal protein induced in the haemolymph of Sarcophaga peregrina (flesh-fly) larvae.
The mode of action of a bactericidal protein (sarcotoxin I) purified from the haemolymph of Sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of the membrane of Escherichia coli. Sarcotoxin I almost completely blocked the uptakes of tetraphenylphosphonium ion and proline, which are known to be driven by a membrane potential, indicating that this protein disrupts the membrane potential of bacteria.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D004175:Diptera / Q000032:analysis*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006458:Hemolymph / Q000032:analysis*; D007301:Insect Hormones / Q000097:blood / Q000494:pharmacology*; D019476:Insect Proteins; D007814:Larva / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D007301:Insect Hormones; D019476:Insect Proteins; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D012967:Sodium Dodecyl Sulfate; C042572:sarcotoxin I protein, Sarcophaga peregrina; D011392:Proline; C013289:tetraphenylphosphonium