Cellular immune reactions against rat pancreatic islets mediated by antibodies from type I diabetic patients.
Serum samples from 13 newly diagnosed type I diabetics were tested for their ability to mediate antibody-dependent cellular immune reactions against pancreatic islets prepared from Wistar/Jena K rats. Nine of these serum samples proved able to mediate islet cell damage when the islets were preincubated with sera and then cultivated with leukocytes of healthy subjects for 20 h. Damage to islets was demonstrated both by the trypan-blue exclusion test and by measurement of insulin leakage during incubation. The data presented point to a possible pathogenetic role of antibodies mediating cellular immune reactions in type I diabetes.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000276:immunology* / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin