Effect of orally administered food-grade carrageenans on antibody-mediated and cell-mediated immunity in the inbred rat.
Experiments were performed to investigate the immunological consequences associated with the persorption of poorly degradable carregeenans from the diet. Using an inbred strain of rat it was demonstrated histochemically, by the carrageenan-specific Alcian blue staining technique, that small quantities of food-grade carrageenans given at 0.5% in drinking-water for 90 days could penetrate the intestinal barrier of adult animals. This apparently occurred via an intact mucosa in the absence of inflammatory or pathological lesions. The carrageenan was demonstrated in macrophage-like cells present within the villi and lamina propria of the small intestine. The oral administration of kappa, lambda or iota food-grade carrageenans did not affect local (biliary) or systemic antibody responses to gut commensal microorganisms, or to orally-administered sheep erythrocytes. However, when sheep red blood cells were administered parenterally the ensuing anti-sheep red blood cell haemagglutinating antibody response was temporarily suppressed in carrageenan-fed rats. lambda-Carrageenan and iota-carrageenan both significantly (P less than or equal to 0.01 and P less than or equal to 0.05, respectively) reduced the mid-phase (14-28 days) haemagglutinin response; kappa-carrageenan (L100) was less effective but caused significant depression at day 21 (P less than or equal to 0.01). Individual responses were, however, within the control range 35 days after sheep erythrocyte administration, thus indicating the temporary nature of this effect. Although carrageenan administration depressed the anti-sheep erythrocyte antibody response, it did not affect T-cell immune competence as measured by the popliteal lymph node assay for graft-versus-host reactivity.
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000917:Antibody Formation / Q000187:drug effects*; D001646:Bile / Q000187:drug effects / Q000276:immunology; D002351:Carrageenan / Q000378:metabolism / Q000633:toxicity*; D004032:Diet; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D006386:Hemagglutination Tests; D007111:Immunity, Cellular / Q000187:drug effects*; D007121:Immunocompetence / Q000187:drug effects; D007070:Immunoglobulin A / Q000032:analysis; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D002351:Carrageenan