[A solid-phase ELISA with specificity for rheumatoid factors of classes IgA, IgG and IgM].
A solid phase ELISA was developed for the simple and rapid determination of rheumatoid factors (RF) of all major immunoglobulin classes. Micro-ELISA-plates were coated with human Fc-fragments and incubated with various dilutions of serum samples as well as with an international RF reference serum. Rheumatoid factors were quantitatively detected by rabbit antibodies (RaHIgA(alpha), RaHIgM(mu), RaHIgG(Fab] conjugated with alkaline phosphatase. The specificity of the ELISA was proved by means of binding inhibition of all rheumatoid factor classes by heat aggregated human IgG. A comparison of the IgM-RF titres of the latex fixation test with the IgM-RF concentration values of the ELISA yielded high correlation (r = .81).
D001172:Arthritis, Rheumatoid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor / Q000276:immunology*
D007070:Immunoglobulin A; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor
Ein Festphasen-ELISA mit Spezifität für Rheumafaktoren der Klassen IgA, IgG und IgM.