Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.
We placed the Saccharomyces cerevisiae GAL4 gene under control of the galactose regulatory system by fusing it to the S. cerevisiae GAL1 promoter. After induction with galactose, GAL4 is now transcribed at about 1,000-fold higher levels than in wild-type S. cerevisiae. This regulated high-level expression has enabled us to tentatively identify two GAL4-encoded proteins.
D001483:Base Sequence; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005690:Galactose / Q000378:metabolism; D005800:Genes, Fungal; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D003470:Culture Media; D005656:Fungal Proteins; D012331:RNA, Fungal; D005690:Galactose