Mechanisms subserving insulin action in the gonad: evidence that insulin induces specific phosphorylation of its immunoprecipitable receptor on ovarian cells.
To test the capacity of insulin to work through a classical insulin-receptor pathway in the ovary, cultured swine granulosa cells were treated with insulin and/or increasing concentrations of insulin-receptor antiserum. Insulin-receptor antiserum but not control serum significantly (greater than 85%) attenuated insulin's stimulation of progesterone biosynthesis. Moreover, in broken-cell preparations, insulin but not desoctapeptide insulin or somatomedins induced specific phosphorylation of the 95,000-dalton, immunoprecipitated beta subunit of the insulin receptor on ovarian cells. These observations provide the first evidence for discrete biochemical actions of insulin at the level of the cell-membrane receptor for insulin in gonadal cells.
D000818:Animals; D002478:Cells, Cultured; D011232:Chemical Precipitation; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D007120:Immunochemistry; D007328:Insulin / Q000502:physiology*; D010053:Ovary / Q000378:metabolism*; D010766:Phosphorylation; D011374:Progesterone / Q000096:biosynthesis; D011972:Receptor, Insulin / Q000378:metabolism*; D013552:Swine
D007328:Insulin; D011374:Progesterone; D011972:Receptor, Insulin