Survival of Campylobacter jejuni in different media and faeces at different temperatures and times of preservation.
Nineteen Campylobacter jejuni strains survived best in suspensions with approximately 10(9) bacteria in 4% albumin solution at-60 degrees C for 17 months (18 out of 19). At the same temperature and during the same period they survived less well in milk (11 out of 19). Recovery of campylobacters after 7 weeks was abundant in albumin, and somewhat less in milk. At -20 degrees C survival in milk was worse; 17 out of 19 cultures did not survive 5 weeks in milk and 7 died in albumin. At 4 degrees C all but one strain survived two weeks in milk and none died in albumin, but after 3 weeks only 7 survived in milk and 2 in albumin. At room temperature campylobacters did not survive 10 days in any of the media used. Peptone water (4%) and peptone-saline proved unsuitable for preservation. From 20 positive stool samples kept at 4 degrees C, on the day after the first sampling C. jejuni could not be recovered from 10 samples; bacteria survived for 2-7 days in 8 samples, for 12-20 days in 2 samples.
D001431:Bacteriological Techniques; D002168:Campylobacter fetus / Q000254:growth & development*; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D011309:Preservation, Biological / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D003470:Culture Media