title: Detection of IgE in the sera of rodents: comparison of the applicability of ELISA and RIA.
abstract: RIA and ELISA were compared for their ability to detect IgE in different rodent species. With a sheep anti-rat IgE antibody good correlation (P less than 0.001) between the 2 assay methods for IgE was found in rats. RIA failed to detect the IgE of Mastomys natalensis while ELISA proved to be a suitable test. However, both tests failed to measure IgE in sera of Nile rats.
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005368:Filariasis / Q000276:immunology; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D008297:Male; D009115:Muridae; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology
chemical_list: D007073:Immunoglobulin E