Development of an enzyme-linked immunosorbent assay for the detection of autoantibodies against thyroglobulin in chickens.
This paper describes the development of an enzyme immunoassay (EIA) for the detection of autoantibodies against thyroglobulin in an avian system. In this system EIA offers an efficient and alternative approach to already established methods such as double diffusion in gel, passive haemagglutination, indirect immunofluorescence and radioimmunoassay. The optimization of the different incubation steps is described and the expression of results of observed antibody activity is discussed.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D001666:Binding Sites, Antibody; D002645:Chickens; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D007124:Immunoenzyme Techniques / Q000592:standards; D007074:Immunoglobulin G / Q000378:metabolism; D011817:Rabbits; D012015:Reference Standards; D013954:Thyroglobulin / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D013954:Thyroglobulin