Survival of granulocytic progenitors in the nonadherent and adherent compartments of human long-term marrow cultures.
We have studied the survival of granulocytic and monocytic progenitor cells (CFU-GM) in human liquid marrow cultures. CFU-GM were present in the nonadherent cell population for a mean of 12 weeks without recharging . Histochemical analysis of agar gels revealed that most day-7 colonies were of neutrophilic type (CFU-N), whereas the majority of day-14 colonies were of mixed neutrophilic-macrophagic type (CFU-NM) for the first four weeks of culture and became predominantly of macrophagic type (CFU-M) thereafter. Eosinophilic colonies (CFU-Eo) declined after week 2 of culture. We have documented that CFU-GM were present in the adherent layer of these cultures, and that the CFU-GM in the nonadherent compartment arise from the adherent layer. In addition, we have compared the pre-CFU-GM survival in the adherent and nonadherent populations and determined that these progenitors were rapidly depleted from both compartments though their survival at the end of week 1 was better in the adherent than in the nonadherent layer.
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000145:classification / Q000166:cytology* / Q000502:physiology; D006651:Histocytochemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000145:classification / Q000166:cytology / Q000502:physiology