High-performance liquid chromatography of insulin.
The elution behaviour of a selection of sequence variant and modified insulins has been compared on a C18 reversed-phase column. Observed elution times were compared with those expected from the nature of the differences from bovine insulin. In some cases prediction rules established for peptides are adequate to explain the observed elution and in others detailed knowledge of the structure of the protein is of considerable importance in understanding elution behaviour.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives / Q000302:isolation & purification*; D051379:Mice; D008958:Models, Molecular; D011384:Proinsulin / Q000302:isolation & purification; D011487:Protein Conformation; D013552:Swine; D014422:Turkeys
D007328:Insulin; D011384:Proinsulin