[Proteolytic cleavage of influenza virus matrix protein and nucleoprotein in an in vitro system].
Proteolytic modification of NP-protein which is a part of influenza virus ribonucleoprotein (RNP) was studied in vitro. NP-protein of influenza virus (mol. weight 56 000) was shown upon RNP treatment with bromeline to undergo proteolytic cleavage revealing 2 more polypeptides with molecular weights of 53 000 and 45 000 daltons. The same classes of NP-proteins were present in RNPs recovered from subviral units obtained by virus treatment with bromeline at buffer pH below 7. Electrophoresis in 12% polyacrylamide gel under reducing conditions of subvirus units obtained by treatment of virions with bromeline at the buffer pH below 7 also demonstrated proteolytic cleavage of M-protein present in them into fragments with molecular weights of 8 500-12 000 daltons. Similar results were obtained upon direct treatment with bromeline of M-protein isolated from virions.
D001963:Bromelains / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000032:analysis / Q000187:drug effects*; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000032:analysis*; D010447:Peptide Hydrolases / Q000494:pharmacology*; D012261:Ribonucleoproteins / Q000032:analysis; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis / Q000187:drug effects
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D001963:Bromelains; D010447:Peptide Hydrolases
Proteoliticheskoe rasshcheplenie matriksnogo belka i belka nukleoproteida virusa grippa v sisteme in vitro.