Isolation of a single messenger RNA and of the corresponding gene in Plasmodium berghei.
A genomic library of Plasmodium berghei DNA was constructed using lambda 47.I as a vector. It represents 90% of Plasmodium genome. Genes expressed during the intraerythrocytic stage of P. berghei were isolated among the recombinant clones of the library using labelled cDNA complementary to the polyA + Plasmodium mRNA extracted during this stage. The purified coding strand of an expressed clone was utilized to catch the corresponding mRNA(s). The hybridized mRNA fraction was eluted and in vitro translated. Translation products were analyzed by gel electrophoresis; the gel fluorography revealed a single protein band of 32.500 daltons of molecular weight, corresponding to a 900bp coding region in the examined clone.
D000818:Animals; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D010962:Plasmodium berghei / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D014158:Transcription, Genetic
D012333:RNA, Messenger