Impaired opsonophagocytosis of serotypes Ib and II of group B streptococci as compared with serotypes Ia and III: role of the alternative pathway of complement in opsonisation of serotype III of group B streptococci.
Using the technique of phagocytic chemiluminescence, we have shown that serotypes Ib and II of group B streptococci are resistant to opsonophagocytosis. The resistant strains became susceptible to opsonophagocytosis by trypsin treatment, but neuraminidase had no effect. Several studies have failed to define a significant role for the alternative pathway of complement in opsonisation of group B streptococci. By simple chelation and heat inactivation studies, we have shown that the alternative pathway of complement is activated by serotype III of group B streptococci.
D000328:Adult; D002648:Child; D003167:Complement Activation / Q000187:drug effects; D003170:Complement Pathway, Alternative / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D012703:Serotyping; D013292:Streptococcus agalactiae / Q000145:classification* / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology
D014361:Trypsin Inhibitors; D008165:Luminol; D009439:Neuraminidase; D014357:Trypsin