Monoclonal antibodies to L-asparaginase.
Five hybridomas secreting monoclonal antibody to E. coli L-asparaginase were isolated. These monoclonal antibodies were classified into 3 different subclasses; Ig G1 (1 clone), Ig G2 (2 clones) and Ig G3 (2 clones). One of them possessed anti-L-asparaginase neutralizing activity. Four antibodies examined demonstrated a linear Langmuir binding plot and binding affinities, with equilibrium dissociation constant (Kd) ranging between 2.5 X 10(-9) M and 6.3 X 10(-10) M. The monoclonal antibodies should be useful probes for investigation of the enzyme activity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001215:Asparaginase / Q000276:immunology*; D004926:Escherichia coli / Q000201:enzymology; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D008297:Male; D051379:Mice
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D001215:Asparaginase