Deletions in Klebsiella pneumoniae R plasmids induced by growth in the presence of acridine orange at high temperature.
The generation in vivo of plasmids deleted at specific sites in strains of Klebsiella pneumoniae containing R plasmids, by treatment with high concentrations of acridine orange (1.2 mg/ml) at 42 degrees C are reported. These deletions seem to be site specific because loss of specific restriction fragments after digestion with restriction enzymes was demonstrated.
D000165:Acridine Orange / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006358:Hot Temperature; D007709:Klebsiella / Q000254:growth & development; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics*; D011815:R Factors / Q000187:drug effects*
D004269:DNA, Bacterial; D000165:Acridine Orange