title: Repair of membrane damage in X-irradiated E. coli.
abstract: When E. coli B/r or E. coli K12 AB1157 were X-irradiated in the presence of oxygen and incubated immediately after irradiation in broth containing penicillin in concentration that on its own was not lethal to unirradiated bacteria, substantial additional killing was caused. When treatment with penicillin was delayed for increasing times after irradiation the additional killing became progressively less. These results were interpreted as demonstrating the repair or removal of oxygen-dependent radiation-induced lesions in the bacterial membranes. Removal of these lesions was inhibited by incubation of the irradiated bacteria at low temperature before treatment with penicillin or by exposing the cells to a non-lethal concentration of toluene before irradiation. These observations suggest that an enzymatic repair process may be involved in the removal of the membrane lesions. The fatty acid mutant E. coli K 1060 proved exceptional in that some additional killing by penicillin was detectable after anaerobic as well as aerobic irradiation. This points to the importance of membrane composition in the development of those radiation lesions that are brought to light by penicillin treatment.
mesh_terms: D002462:Cell Membrane / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects*; D006358:Hot Temperature; D009154:Mutation; D010100:Oxygen; D010406:Penicillins / Q000494:pharmacology*; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology
chemical_list: D010406:Penicillins; D014050:Toluene; D010100:Oxygen