In vivo localization of insulin binding to cells of the rat pancreas.
The uptake of 125I-insulin by rat pancreas was studied in vivo. Following fixation and light microscope autoradiography, saturable uptake of 125I-insulin was quantitatively demonstrated on acinar and duct cells but not on blood vessels and islets of Langerhans. Electron microscopy revealed the localization of 125I-insulin to the basolateral cell membranes of acinar and duct cells.
D000818:Animals; D001345:Autoradiography; D007328:Insulin / Q000031:analogs & derivatives* / Q000378:metabolism; D007457:Iodine Radioisotopes; D008297:Male; D008854:Microscopy, Electron; D010179:Pancreas / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D007457:Iodine Radioisotopes; C008147:insulin, iodo-; D011972:Receptor, Insulin