[Determination of cholera enterotoxin in supernatants of homogenates from the wall of the small intestine in rabbits in the passive immune hemolysis reaction].
The article presents the data indicating that suckling rabbits can be used as a model, specific only for enterotoxin-producing Vibrio eltor strains. The signs characteristic of low virulence may appear as the consequence of the action of other products resulting from the vital activity of vibrios. No strains capable of producing toxin only in vivo or in vitro have been detected. The quantitative correlation of the synthesis of enterotoxin in the body of experimental animals and in vitro has been established. The passive immune hemolysis test can be used as an objective method suitable for the determination of the choleragenic potency of vibrios.
D000818:Animals; D000833:Animals, Suckling; D004768:Enterotoxins / Q000032:analysis*; D006462:Hemolytic Plaque Technique; D007413:Intestinal Mucosa / Q000032:analysis; D007421:Intestine, Small / Q000032:analysis*; D011817:Rabbits; D014734:Vibrio cholerae / Q000472:pathogenicity; D014774:Virulence
D004768:Enterotoxins
Opredelenie kholernogo énterotoksina v supernatantakh gomogenatov stenki tonkoĭ kishki krolika v reaktsii passivnogo immunogo gemoliza.