Purification and characterisation of a 12 K dalton peptide with an affinity for oriC containing DNA fragments from Escherichia coli K12 membrane fractions.
A DNA binding protein capable of interacting specifically with oriC containing DNA was purified to near homogeneity from E. coli membrane fractions. It has a molecular weight of 12 KD in denaturing conditions. The specific binding to oriC DNA is more resistant to the presence of salt than the non specific binding. Neither the DNA binding activity nor the specificity for oriC DNA was destroyed by heating at 100 degrees C for 2 min.
D002462:Cell Membrane / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009994:Osmolar Concentration; D010957:Plasmids; D011485:Protein Binding; D012965:Sodium Chloride / Q000494:pharmacology
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012965:Sodium Chloride