title: Evidence for an alpha-melanocyte stimulating hormonergic hippocampal commissural connection in the rat, revealed by a double-labeling technique.
abstract: A new double-labeling method combining immunocytochemistry and a retrograde tracer technique using biotin-conjugated horseradish peroxidase (B-HRP) was developed. Retrogradely accumulated B-HRP was visualized in the soma by incubation with avidin-conjugated fluorescein isothiocyanate (FITC-green fluorescein) solution. Texas red (red fluorescein)-conjugated goat anti-rabbit IgG was used for simultaneous demonstration of the antigen. Using this method, the present study reports a newly demonstrated commissural hippocampal alpha-melanocyte stimulating hormonergic neuron system in the rat.
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism*; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism*; D009434:Neural Pathways / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009074:Melanocyte-Stimulating Hormones