Response of monkeys to porcine zona pellucida as detected by a solid-phase radioimmunoassay.
The immunological response of cynomolgus monkeys (Macaca fascicularis) to immunization was evaluated utilizing collagenase-isolated pig zona pellucida. Six weeks after initial immunization a high serum titer of antibody was exhibited. Serum antibody titers demonstrated a noticeable decline 5 months after booster injections were discontinued. The assay method used is rapid and is capable of detecting antibody in serum dilutions of 1:78,000, as compared to 1:125,000 with the indirect fluorescence assay.
D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D006224:Cricetinae / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D007114:Immunization; D007117:Immunization, Secondary; D008251:Macaca / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D051379:Mice / Q000276:immunology; D010063:Ovum / Q000276:immunology*; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000276:immunology; D013552:Swine / Q000276:immunology; D015044:Zona Pellucida / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D013205:Staphylococcal Protein A; D000536:Aluminum Hydroxide