title: Confirmation of EMIT cannabinoid assay results by bonded phase adsorption with thin layer chromatography.
abstract: Clinical urine specimens were screened for the presence of cannabinoids using the EMIT Cannabinoid Assay. Aliquots of these samples were also analyzed for 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THCA), the major cannabis metabolite in urine, by a technique which combines bonded phase adsorption (BPA) and thin layer chromatography (TLC). A 100% agreement between EMIT and BPA-TLC results was observed when at least 20 mL of urine was assayed by BPA-TLC. Bonded phase adsorption coupled with thin layer chromatography appears to be a suitable technique for the confirmation of positive EMIT Cannabinoid Assay results.
mesh_terms: D000327:Adsorption; D002186:Cannabinoids / Q000652:urine*; D002793:Cholic Acids / Q000652:urine; D002855:Chromatography, Thin Layer / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D002186:Cannabinoids; D002793:Cholic Acids; C003681:3,7,12-trihydroxycoprostanic acid