title: Glial fibrillary acidic protein and differentiation of neonatal rat pituicytes in vitro.
abstract: The appearance and intracellular localisation of glial fibrillary acidic protein (GFAP) in pituicytes in neural lobe cultures of newborn rats aged 7 to 30 days were investigated by use of the indirect immunofluorescence method. GFAP-immunoreactive cells were observed mostly in the outgrowth zone. GFAP was localised in the perikaryal cytoplasm as well as in pituicyte processes. GFAP-positive pituicytes showed considerable morphological polymorphism. The presence of GFAP - astrocytic marker - in pituicytes in vitro and the evident morphological similarity to cultured astrocytes suggest the astroglial character of these cells.
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010904:Pituitary Gland, Posterior / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein