The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids.
The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their presence in strains being monitored on galactose fermentation indicator media.
D001431:Bacteriological Techniques; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000502:physiology*; D005690:Galactose / Q000378:metabolism; D005819:Genetic Markers; D011815:R Factors
D004269:DNA, Bacterial; D005819:Genetic Markers; D005690:Galactose