title: Concurrent visualization of choline acetyltransferase-like immunoreactivity and retrograde transport of neocortically injected markers in basal forebrain neurons of cat and rat.
abstract: Magnocellular neurons in the basal forebrain of rats and cats were retrogradely labeled with Fast Blue or horseradish peroxidase injected into the neocortex. Using antisera against choline acetyltransferase (ChAT) a direct double-labeling technique was carried out and it was demonstrated that retrogradely transported markers and ChAT-like immunoreactivity occur within the same neurons. These findings strongly support the cholinergic nature of basal forebrain projection to the neocortex.
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology*; D001931:Brain Mapping; D002415:Cats; D002540:Cerebral Cortex; D002795:Choline O-Acetyltransferase / Q000276:immunology*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000008:administration & dosage; D007124:Immunoenzyme Techniques; D051381:Rats; D014018:Tissue Distribution
chemical_list: D006735:Horseradish Peroxidase; D002795:Choline O-Acetyltransferase