Protease inhibitor localization in control and streptozotocin-diabetic skeletal muscles.
alpha 1-Antitrypsin and alpha 1-inhibitor-3 were localized for the first time inside skeletal muscle cells. Their content, especially that of alpha 1-inhibitor-3, was greatly reduced following streptozotocin-induced diabetes. alpha 1-Antitrypsin and alpha 1-inhibitor-3 were also observed in the vascular components and interstitial space surrounding both control and diabetic soleus muscles as revealed by immunofluorescence. In diabetic muscles, the non-myofibre locale of alpha 1-inhibitor-3 was reduced, and to a lesser extent, alpha 1-antitrypsin. Both myofibre and extracellular patterns were reversed to control levels by insulin replacement.
D000209:Acute-Phase Proteins; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004705:Endocytosis; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D008297:Male; D009132:Muscles / Q000201:enzymology*; D011480:Protease Inhibitors / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D000515:alpha 1-Antitrypsin / Q000032:analysis
D000209:Acute-Phase Proteins; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; C017894:alpha 1-inhibitor 3