title: Studies of enterotoxin of Shigella dysenteriae type 1. I. Effect of aeration on the production of enterotoxin and suitability of laboratory animals for its biological assay.
abstract: Shigella dysenteriae type 1 was used to study the influence of aeration on enterotoxin production and the suitability of laboratory animals for the toxin assay. The enterotoxin produced under aerobic shake culture conditions gave a gut dilatory response of 1.436 but that obtained in aerobic and anaerobic stationary cultures exerted no activity. The toxin fractionated on Sephadex G-150 column was more effective than that obtained by ammonium sulphate precipitation. Only Sephadex column fraction II had a toxic activity. Among the three laboratory animal models tested, the rabbit ileal loop was suitable for the assay of toxin. The skin permeability test was unsatisfactory for enterotoxicity assay.
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001681:Biological Assay; D004768:Enterotoxins / Q000096:biosynthesis* / Q000633:toxicity; D007082:Ileum / Q000502:physiology; D010539:Permeability; D011817:Rabbits; D051381:Rats; D012762:Shigella dysenteriae / Q000378:metabolism*; D012879:Skin Physiological Phenomena
chemical_list: D004768:Enterotoxins