Immunochemical and immunohistochemical studies on the specificity of a monoclonal antibody to choline acetyltransferase of rat brain.
Choline acetyltransferase (ChAT) preparations partially purified from brains of rat, mouse and cat were electrophoresed in polyacrylamide gels and blotted on nitrocellulose sheets. A monoclonal antibody, Ab-57, bound specifically to one protein band which coincided with the peak of ChAT activity in each species. Using Ab-57, ChAT was demonstrated immunohistochemically in cholinergic structures in rat, mouse and cat brains. Thus, the specificity and cross-species reactivity of the monoclonal antibody Ab-57 have been established.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D001921:Brain / Q000201:enzymology*; D002415:Cats; D002795:Choline O-Acetyltransferase / Q000276:immunology*; D002799:Cholinergic Fibers / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase