Immunocytochemical detection in eel corpuscles of Stannius of a mammalian parathyroid-like hormone.
Stannius corpuscles of the eel synthesize and secrete a mammalian parathyroid-like hormone called parathyrin of CS (PCS). PCS has been localized in the cytoplasm of all cells in the corpuscles, detection being by indirect immunofluorescence with an antiserum anti-1-84 bovine hormone (PTH). The specificity of the reaction was demonstrated by inhibition of the fluorescent staining with 1-84 bovine PTH and the active fragment 1-34 of human PTH. Variations of the cellular localization of the PCS or a complete depletion of the hormonal content, in all cells, were observed in eels made hypercalcemic by Ca overloading. The secretory activity of the two types of CS cells may be regulated by the plasma Ca2+ concentration.
D000818:Animals; D002118:Calcium / Q000502:physiology; D003594:Cytoplasmic Granules / Q000378:metabolism; D004524:Eels / Q000378:metabolism*; D004702:Endocrine Glands / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D010281:Parathyroid Hormone / Q000032:analysis / Q000378:metabolism*; D051381:Rats
D010281:Parathyroid Hormone; D002118:Calcium