Two autonomously replicating sequences near oli-1 gene of yeast mitochondrial DNA.
We cloned two autonomously replicating sequences from a short segment of mtDNA of an oligomycin-resistant petite yeast, O-111, into a vector pYleu 12 constructed from yeast LEU 2 gene and pBR 322. These plasmids, pYmit 4 and pYmit 1, had frequencies of transformation of yeast as high as that of YEp 13, having a replicator of 2 mu DNA. They were maintained as plasmids in yeast under selective conditions and shuttled from yeast to E. coli. No evidence was obtained that these plasmids were incompatible with the wild-type mitochondrial genome. These sequences were located in intergenic regions.
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004272:DNA, Mitochondrial