Localization of LDL in arteries: improvements in immunofluorescence procedures.
We have described the development of procedures used to localize LDL in arteries with atherosclerotic lesions from humans and experimental animal models. We have first illustrated data obtained from earlier cryostat studies, described the procedures, given examples of results of LDL localization in sections of paraffin-embedded blocks, and finally outlined the procedures used and examples for LDL localization in epoxy-embedded blocks. Future improvements in resolution of LDL localization will probably require performing electron microscopy on ultrathin sections of epoxy-embedded arteries followed by immunocytochemistry.
D000818:Animals; D001158:Arteries / Q000378:metabolism; D001161:Arteriosclerosis / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000276:immunology / Q000378:metabolism*; D013552:Swine
D008077:Lipoproteins, LDL