title: Immunocytochemical evidence for the presence of oxytocin and neurophysin in the large cells of the bovine corpus luteum.
abstract: The presence of neurophysin, oxytocin and vasopressin in the bovine corpus luteum was examined immunocytochemically. Tissue blocks of corpora lutea from pregnant and non-pregnant animals were fixed with glutaraldehyde/paraformaldehyde fixative and immunostained by the peroxidase-antiperoxidase (PAP) method. The simultaneous presence of immunoreactive oxytocin and immunoreactive oxytocin-neurophysin was demonstrated in large luteal cells of non-pregnant animals, while no staining for vasopressin or vasopressin-neurophysin was observed. None of the peptides were detected in the corpus luteum of pregnant animals. The small luteal cells were not found to be stainable at any time.
mesh_terms: D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D007158:Immunologic Techniques; D009481:Neurophysins / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism*; D011247:Pregnancy; D014667:Vasopressins / Q000378:metabolism
chemical_list: D009481:Neurophysins; D014667:Vasopressins; D010121:Oxytocin