Comparison of the API Staph-Ident and DMS Staph-Trac systems with conventional methods used for the identification of coagulase-negative staphylococci.
Two commercial kit systems, the API Staph-Ident system (Analytab Products, Inc., Plainview, N.Y.) and the DMS Staph-Trac system (DMS Laboratories, Inc., Flemington, N.J.), were compared with conventional methods for the identification of nine species of coagulase-negative staphylococci. The API Staph-Ident system, a biochemical and chromogenic substrate micromethod, correctly identified 95 of 120 (79.2%) clinical isolates of coagulase-negative staphylococci after 5 h of incubation. The DMS Staph-Trac system, a miniaturized biochemical test system which requires a 24-h incubation period, correctly identified 106 (88.3%) of the same isolates. Both commercial systems were similar in cost and amount of technologist time required to inoculate and read each system. The clinical value of routine species identification of coagulase-negative staphylococci has not yet been established. The decision by clinical laboratories of whether to adopt this practice will be greatly facilitated by the availability of commercial kit systems which are both rapid and accurate.
D000469:Alkaline Phosphatase / Q000378:metabolism; D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D003030:Coagulase / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000145:classification*; D014510:Urease / Q000378:metabolism
D003030:Coagulase; D000469:Alkaline Phosphatase; D014510:Urease