Combined immunocytochemistry and autoradiographic retrograde axonal tracing for identification of transmitters of projection neurons.
A double labeling method is described which combines immunocytochemistry for identification of the neurotransmitter serotonin with autoradiographic retrograde axonal tracing using wheat germ agglutinin (WGA), N-[acetyl-3H]. The permanence, sensitivity, and distinctness of the two labels provide a valuable means for analyzing transmitter-identified projection neurons in the central nervous system. Combined immunohistochemical/autoradiographic preparations, following injections of WGA, N-[acetyl-3H] in the caudate-putamen of mice, revealed large numbers of serotonergic and fewer non-serotonergic raphe-striatal projection neurons.
D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000648:ultrastructure*; D001921:Brain / Q000033:anatomy & histology*; D003342:Corpus Striatum / Q000033:anatomy & histology; D003712:Dendrites / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D037102:Lectins; D008636:Mesencephalon / Q000033:anatomy & histology; D051379:Mice; D008813:Mice, Inbred ICR; D009434:Neural Pathways / Q000033:anatomy & histology; D011903:Raphe Nuclei / Q000033:anatomy & histology; D012701:Serotonin / Q000378:metabolism*; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins; D012701:Serotonin