High affinity monoclonal antibodies to luteinizing hormone-releasing hormone. Preparation and binding studies.
Two mouse hybridoma cell lines (50-1 and 50-11) secreting high affinity, monoclonal IgG antibodies (MCAs) against luteinizing hormone-releasing hormone (LHRH) have been established. Measurements of binding affinity by both equilibrium and kinetic methods give dissociation constants (Kd) of 2.3-3.2 X 10(-10) M for 50-1 and 3.0 X 10(-10) M for 50-11. Analysis of binding specificity show that both MCAs require the C-terminal glycine amide and adjacent 4 amino acids of LHRH for binding. The use of these MCAs in radioimmunoassay (RIA) is described.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis / Q000276:immunology*; D007031:Hypothalamus / Q000032:analysis; D007700:Kinetics; D051379:Mice
D000911:Antibodies, Monoclonal; D007987:Gonadotropin-Releasing Hormone