Gonadotropin-releasing hormone (Gn-RH) in striped mullet (Mugil cephalus), milkfish (Chanos chanos), and rainbow trout (Salmo gairdneri): comparison with salmon Gn-RH.
Immunoreactive gonadotropin-releasing hormone (Gn-RH) was extracted from brains of striped mullet, milkfish, rainbow trout, and chum salmon with acetone/HCl and petroleum ether. High pressure liquid chromatography and cross-reactivity studies show mullet, milkfish, and trout brains to contain a peptide chromatographically and immunologically identical to synthetic salmon Gn-RH, while the mammalian form of Gn-RH is detectable in none of these fishes. Gn-RH is present in immature 7-month-old and 4-year-old milkfish. A second immunoreactive peptide is separable by HPLC in all the fish studied. This "early-eluting" form of Gn-RH is unlikely to be a precursor; its cross-reactivity with antisera R-42 and #185 suggests that any modification is in the C-terminal region. Several possible roles for this peptide are advanced.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D005260:Female; D005399:Fishes / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010906:Pituitary Hormone-Releasing Hormones / Q000378:metabolism*; D011863:Radioimmunoassay; D012474:Salmon / Q000378:metabolism; D013045:Species Specificity; D014337:Trout / Q000378:metabolism
D007457:Iodine Radioisotopes; D010906:Pituitary Hormone-Releasing Hormones