title: The bacteriophage Mu gene B product is incorporated into the inner membrane of Escherichia coli.
abstract: By using the Triton X-114 method, it could be shown that an Mr 33 000 protein expressed from the left-end EcoRI-C fragment of phage Mu behaves like a hydrophobic protein. Fusion of a DNA region coding for this protein to the controllable pL promoter of phage lambda results in the production of large amounts of the 33-kDal protein under inducing conditions. This protein can be isolated in association with the inner membrane of Escherichia coli cells. Based on complementation studies it was concluded that the 33-kDal protein is the product of the early gene B.
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005796:Genes; D005814:Genes, Viral; D005816:Genetic Complementation Test; D005838:Genotype; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009876:Operon; D010957:Plasmids; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D014764:Viral Proteins; C043426:inner membrane protein, E coli