[Determination of tularemia antibodies by an immunoenzyme method on a solid-phase carrier (ELISA)].
ELISA "sandwich" techniques have been developed and the optimum assay conditions for detecting specific antibodies in human serum samples have been determined. The possibility of using these techniques for the determination of the level of antibodies to tularemia antigens in the sera of persons immunized with live tularemia vaccine has been shown. Statistically significant differences in the level of antibodies to tularemia antigen in the sera of immunized and nonimmunized persons have been established. The comparative study of five serological methods - ELISA, the agglutination test, the passive hemagglutination test, the immunofluorescence test and the defined antigen substrate sera ( DASS ) techniques - has revealed the advantage of ELISA, whose sensitivity has proved to be considerably higher than that of all other methods used in our work.
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D001428:Bacterial Vaccines / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005604:Francisella tularensis / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D013997:Time Factors
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines
Opredelenie tuliaremiĭnykh antitel immunofermentnym metodom na tverdofaznom nositele (ELISA).