[Evidence for the existence of inactive arterial renin in the rat].
Inactive renin in rat arterial walls was investigated according to the following experiments. Dialysis at pH 7.4 following dialysis at pH 3.3 of the arterial tissue resulted in a significant rise of renin activity, from a control value of 0.41 +/- 0.07 to 0.62 +/- 0.06 ng/ml/h (p less than 0.01). Treatment with trypsin of the arterial tissue caused a rapid and apparent increase in the renin activity at either 0 or 27 degrees C. The molecular weight of the active renin was estimated to be 32,000 or 39,000, while that of the inactive renin was found to be 36,000 or 44,000 on Sephadex G-100 gel filtration. The contents of the inactive renin varied with different segments of arterial wall. The ratio of inactive renin to total renin was the lowest in renal artery wall (0.32), while there was no significant difference in the ratio in other arterial walls (abdominal aorta, 0.87; thoracic aorta, 0.93; carotid artery, 0.96; mesenteric artery, 0.89; pulmonary artery, 0.92). These findings suggest that conversion of inactive renin into active renin can occur in arterial tissue, which, in turn, plays an important role in the local control of vascular tone. It seems that inactive renin found in the arterial wall is of local origin.
D000818:Animals; D001012:Aorta, Abdominal / Q000032:analysis; D001013:Aorta, Thoracic / Q000032:analysis; D001158:Arteries / Q000032:analysis*; D002339:Carotid Arteries / Q000032:analysis; D003080:Cold Temperature; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000097:blood*; D008297:Male; D008638:Mesenteric Arteries / Q000032:analysis; D011651:Pulmonary Artery / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood* / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D014357:Trypsin; D012083:Renin