Immunorecognition of anti-serotonin antibodies by using a radiolabelled ligand.
Specificity of anti-serotonin antibodies to be assayed for immunocytochemical studies was tested in vitro by using a radiolabelled ligand mimicking the hapten structure. A good immunorecognition was found after coupling [3H]HT to a lysine with formaldehyde. Competition experiments by equilibrium dialysis were then carried out between [3H]HT-F-ALM and both conjugated and unconjugated indoles. The cross-reactivity ratios showed that the antibody recognition, though less specific than expected, was found suitable for immunocytochemical applications.
D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000378:metabolism; D003429:Cross Reactions; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000276:immunology* / Q000378:metabolism
D012701:Serotonin