A revision of the Dictyostelium discoideum cell cycle.
We have investigated the Dictyostelium discoideum cell cycle using fluorometric determinations of cellular and nuclear DNA contents in exponentially growing cultures and in synchronized cultures. Almost all cells are in G2 during both growth and development. There is no G1 period, S phase is less than 0.5 h, and G2 has an average length of 6.5 h in axenically grown cells. Mitochondrial DNA, which constitutes about half of the total DNA, is replicated throughout the cell cycle. There is no difference in the nuclear DNA contents of axenically grown and bacterially grown cells. Thus the long cell cycle in axenically grown cells is due to a lengthening of the G2 phase.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000032:analysis; D003584:Cytological Techniques; D004261:DNA Replication; D004271:DNA, Fungal / Q000032:analysis; D004023:Dictyostelium / Q000032:analysis / Q000166:cytology*; D005434:Flow Cytometry; D005470:Fluorometry; D007399:Interphase; D008928:Mitochondria / Q000032:analysis
D004271:DNA, Fungal