The distribution of alpha-melanocyte stimulating hormone (alpha-MSH) in the central nervous system of the rat: an immunohistochemical study. II. Lower brain stem.
The distribution of immunoreactive alpha-melanocyte stimulating hormone (alpha-MSHI) in the rat lower brain stem was examined by indirect immunofluorescence or peroxidase- anti-peroxidase immunohistochemical method using an antiserum against synthetic alpha-MSH. The results confirmed the presence of alpha-MSHI fibers in the midbrain central gray matter and parabrachial area, and demonstrated a much more extensive distribution of these fibers in various parts of the lower brain stem areas previously thought not contain alpha-MSHI fibers. In addition, the commissural nucleus was identified as a new alpha-MSHI neurons-containing site. No alpha-MSHI neurons were seen in other regions of the rat lower brain stem.
D000818:Animals; D001933:Brain Stem / Q000032:analysis / Q000033:anatomy & histology* / Q000166:cytology; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D009074:Melanocyte-Stimulating Hormones