title: Comparison of two screening tests for Streptococcus mutans and evaluation of their suitability for mass screenings and private practice.
abstract: Two simple microbiological tests for quantitating salivary Streptococcus mutans levels were compared with each other and evaluated for suitability for mass screenings and private practice. Both tests use mitis salivarius medium with bacitracin (MSB) and are selective for S. mutans. One test estimates colonies grown on agar (MSBA) and the other estimates colonies grown in broth that adhere to glass (MSBB). Both are scored from 1 (lowest) to 5 (highest). Children (293) in grades 5 and 6 in Tallahassee, Florida (nonfluoridated) were tested for S. mutans levels. Scores of children by both tests were significantly similar (chi 2, regression and Kappa statistical analysis). Correlation coefficients (regression) between scores and DMFS increments of the previous 4 yr were 0.35 (MSBA) and 0.26 (MSBB). Both tests were very good in identifying children with low caries increments, but positive scores did not correlate well with high caries increments. Sampling and interpreting took 2 min (MSBA) and 1 min (MSBB). Cost of MSBA/child was $1.15, and MSBB/child $1.25. These tests are economical and suitable for mass screenings to identify low risk populations who do not require preventive treatment.
mesh_terms: D001431:Bacteriological Techniques; D002648:Child; D004245:DMF Index; D003732:Dental Caries Activity Tests / Q000379:methods; D003733:Dental Caries Susceptibility; D005431:Florida; D006801:Humans; D008403:Mass Screening / Q000191:economics / Q000379:methods*; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000302:isolation & purification*; D014505:Urban Population