Production and characterization of monoclonal antibodies to the myelin glycolipid sulfatide.
Sulfatide is enriched in the myelin sheath and accounts for 5% of the total lipids in this membrane. In the present work we describe the production and characterization of mouse monoclonal antibodies against sulfatide. The antibodies were detected and characterized in a previously described ELISA test system. The clone AIC3IA2 produced antibodies of the IgG3 class with high specificity for sulfatide. These antibodies showed almost no cross-reactivity with galactocerebroside or with any of the other lipids we tested. When used with the peroxidase antiperoxidase technique the antibodies stained a cell population either in fixed or unfixed brain cell cultures, indicating a surface localization of sulfatide in the respective cell population. In double-staining experiments the stained cell population was identified as myelin basic protein-positive oligodendrocytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008563:Membrane Lipids / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009186:Myelin Sheath / Q000276:immunology*; D009836:Oligodendroglia / Q000378:metabolism; D013433:Sulfoglycosphingolipids / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D008563:Membrane Lipids; D013433:Sulfoglycosphingolipids