[Immunogenicity of inactivated Klebsiella ozaenae cultures in relation to the properties and methods of culturing and preserving the initial strains].
Experiments in the active protection of mice from generalized K. ozaenae infection have demonstrated that the heat-killed cultures of K. ozaenae capsular strains (antigens 02B : K4) possess pronounced and stable immunogenic properties, dependent on the presence and type of the capsular antigen and independent of the virulence and age of the initial strain, as well as the time and methods of its cultivation (the type of the culture medium: nutrient agar, glucose-mineral medium) and storage (the term of observation is 2 years). This investigation has resulted in the determination of the strain (2211) with the highest and most stable protective properties and in the selection of the optimum conditions for the immunization of mice (by the subcutaneous injection of 250 microbial bodies per mouse) with its heat-killed culture.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D001431:Bacteriological Techniques; D004306:Dose-Response Relationship, Immunologic; D004353:Drug Evaluation, Preclinical; D007114:Immunization; D007709:Klebsiella / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D011309:Preservation, Biological / Q000379:methods; D012222:Rhinitis, Atrophic / Q000517:prevention & control; D014613:Vaccines, Attenuated / Q000276:immunology; D014774:Virulence
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated
Immunogennost' inaktivirovannykh kul'tur Klebsiella ozaenae v zavisimosti ot svoĭstv, metodov kul'tivirovaniia i khraneniia iskhodnykh shtammov.