Isolation of adenosine 5'-diphosphate-L-glycero-D-mannoheptose, the assumed substrate of heptose transferase(s), from Salmonella minnesota R595 and Shigella sonnei Re mutants.
From heptose transferase-less Re mutants of Salmonella minnesota and Shigella sonnei, a mixture of nucleotide-linked heptoses was isolated. After paper chromatography in different solvent systems, ADP derivatives of D-glycero-D-mannoheptose and L-glycero-D-mannoheptose could be isolated in pure form. The structure of ADP-L-glycero-D-mannoheptose was verified by analytical methods and by transformation of ADP-D-glycero-D-mannoheptose with ADP-D-glycero-D-mannoheptose-6-epimerase.
D000247:Adenosine Diphosphate Sugars / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D009154:Mutation; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification*; D012475:Salmonella / Q000201:enzymology* / Q000235:genetics; D012764:Shigella sonnei / Q000201:enzymology* / Q000235:genetics; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D000247:Adenosine Diphosphate Sugars; D009702:Nucleoside Diphosphate Sugars; C031557:adenosine 5'-diphosphate-glycero-mannoheptose; D014166:Transferases