title: Monoclonal antibodies recognising cell surface molecules expressed by rat cerebellar interneurons.
abstract: We describe monoclonal antibodies recognising three cell surface antigens expressed by rat cerebellar granule neurons. The antibodies were produced after immunising mice with a cerebellar glycoprotein preparation. 6-1-3 recognises a glycoprotein with an apparent molecular weight of 144 000 daltons, and gives an unusual staining pattern on cultured neurons. 7-8D2 recognises postmitotic granule cells, but not their precursors in the external granular layer. The antigen was not detected in any brain region other than the cerebellum. 8-20-1 recognises a brain specific glycoprotein with an apparent molecular weight of 48 000 daltons, which appears on cerebellar granule cells only after the 10th postnatal day.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001253:Astrocytes / Q000276:immunology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000276:immunology*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D007395:Interneurons / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface