Detection of tumour-associated transplantation antigens by syngeneic cytotoxic T lymphocytes after fusion of tumour cell membrane vesicles to recipient cells.
Sendai virus envelope-mediated fusion was used to transfer plasma membrane vesicles of ESb lymphoma cells (H-2d) to syngeneic or allogeneic recipient cells. After fusion, ESb-specific cell surface determinants were detectable on the recipient cells, as revealed by indirect immunofluorescence and cytofluorographic analysis. Fused cells were also tested as targets in 51Cr-release assays, using syngeneic DBA/2 anti-ESb- or anti-H-2d-specific T lymphocytes (CTL). Fused recipient cells became susceptible to lysis by both types of CTL, indicating that both tumour-specific and H-2d determinants were inserted on fusion. The experimental approach presented could be of help as an assay system for the molecular identification of tumour-specific transplantation antigens.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis*; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic
D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens