Growth and morphology of neuronal cell lines cultured in perfusion.
To optimize culture conditions and gain a more reliable culturing system for studies of metabolic properties of neuronal cells, a simplified perfusion chamber was developed. It consists of two parts: a perfusion block and a standard plastic culture dish. To confirm the suitability of this chamber for continuous culturing of anchorage-dependent cells, the growth and morphology of the four neuronal cell lines, glioma C6 and glioma 138MG, neuroblastoma C1300, clones N1E115 and N18 were followed for 4 d using both traditional and perfusion techniques. A marked increase in growth and a decrease in the degree of morphological differentiation were obtained with the latter technique compared to the former.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D005910:Glioma / Q000473:pathology; D006652:Histological Techniques / Q000295:instrumentation; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000166:cytology*
D003470:Culture Media