Metabolism of amino acids, protein and glucose in fat pads of traumatized rats.
Soft tissue injury to one hindlimb of rats was used to test the response to trauma of metabolism in epididymal fat pads. Degradation of [1-14)C]leucine was lower on day 2 after injury, but not on days 1 or 3, whether or not glucose or insulin were provided. Although trauma did not affect the basal rate of release of 14CO2, lactate or pyruvate from fat pads incubated with [U-14C] glucose, the stimulation by insulin of these processes was smaller in fat pads of 2 day traumatized than of normal animals. These results suggest that trauma due to injury may decrease the capacity for utilization of leucine and glucose by adipose tissue. Release of alanine, glutamine and glutamate by fat pads incubated with leucine was also lower on day 2. This decreased efflux could not be accounted for by changes in net protein breakdown or in pyruvate availability and probably reflected their reduced de novo synthesis due to the diminished release of nitrogen from leucine.
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007658:Ketone Oxidoreductases / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D009132:Muscles / Q000293:injuries; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014947:Wounds and Injuries / Q000378:metabolism*
D007328:Insulin; D009097:Multienzyme Complexes; D011506:Proteins; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D007931:Leucyl Aminopeptidase; D007930:Leucine; D005947:Glucose