A routine immuno-electron microscopic technique for localizing an auto-antibody on epidermal basement membrane.
A new diagnostic technique is described, in which 0.7 mm thick slices of skin are freshly cut, thoroughly washed, slightly fixed and directly incubated in peroxidase-labelled antibodies. This easy technique allows routine immuno-electron microscopic diagnosis of subepidermal auto-immune bullous diseases, with excellent morphological results.
D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000175:diagnosis; D001485:Basement Membrane / Q000276:immunology; D003176:Complement C3 / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008854:Microscopy, Electron; D010390:Pemphigoid, Benign Mucous Membrane / Q000175:diagnosis* / Q000276:immunology; D010391:Pemphigoid, Bullous / Q000175:diagnosis* / Q000276:immunology; D012867:Skin / Q000276:immunology*; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis*
D001323:Autoantibodies; D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G