Lymphocytic choriomeningitis virus. VII. Structural alterations of the virion by treatment with proteolytic enzymes without loss of infectivity.
Treatment of lymphocytic choriomeningitis virus with proteolytic enzymes, hyaluronidase, and phospholipase C increased infectious titres. Biochemical analysis of bromelain- and trypsin-treated virus revealed that infectivity was high in spite of the decrease to low or undetectable levels of all viral glycoproteins as well as partial degradation of the nucleoprotein.
D000956:Antigens, Viral / Q000032:analysis; D006023:Glycoproteins / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D008217:Lymphocytic choriomeningitis virus / Q000187:drug effects / Q000254:growth & development*; D010447:Peptide Hydrolases / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins; D010738:Type C Phospholipases; D006821:Hyaluronoglucosaminidase; D010447:Peptide Hydrolases