[Cleavage of tRNA and rRNA at 7-methylguanine in the presence of methylated carrier RNA].
A method of site-specific cleavage of some tRNAs and rRNAs at the 7-methylguanine residue is described. After reduction of 7-methylguanine by sodium borohydride treatment in the presence of the exogenous carrier RNA methylated statistically by dimethylsulfate the polynucleotide chain is cut at the modified residue by aniline. It was shown that the previously used procedure which did not involve a methylated carrier RNA is not applicable for splitting rRNAs or low concentrations of tRNAs. The method described was successfully used for site-specific cleavage of 32P-terminally labelled yeast tRNAPhe and unlabelled E. coli 16S rRNA at 7-methylguanine position.
D000814:Aniline Compounds; D001894:Borohydrides; D004926:Escherichia coli; D006147:Guanine / Q000031:analogs & derivatives*; D012315:RNA Caps; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012441:Saccharomyces cerevisiae
D000814:Aniline Compounds; D001894:Borohydrides; D012315:RNA Caps; D012335:RNA, Ribosomal; D006147:Guanine; C008450:7-methylguanine; C025364:sodium borohydride; D012343:RNA, Transfer; C023650:aniline
Rasshcheplenie tRNK i rRNK po 7-metilguaninu v prisutstvii metilirovannoĭ RNK--nositelia.