Serological specificity of the liposome lysis test for measurement of anti-ganglioside antibodies. A comparison with hemagglutination inhibition.
A simple and reliable complement-dependent liposome lysis test for the detection of anti-ganglioside antibodies is described. For sera raised in rabbits against the monosialoganglioside NG-GM3, the sensitivity and specificity of antibody detection was compared with that of the HRBC hemagglutination-inhibition test: the liposome lysis test appears more sensitive. A difference in antigen presentation was also demonstrated.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D005679:G(M3) Ganglioside / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006736:Horses; D006801:Humans; D007158:Immunologic Techniques; D007519:Isoantigens / Q000276:immunology; D008081:Liposomes / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D000954:Antigens, Surface; D005679:G(M3) Ganglioside; D005732:Gangliosides; D007519:Isoantigens; D008081:Liposomes