Structural and functional properties of the non-muscle tropomyosins.
The non-muscle tropomyosins (TMs), isolated from such tissues as platelets, brain and thyroid, are structurally very similar to the muscle TMs, being composed of two highly alpha-helical subunits wound around each other to form a rod-like molecule. The non-muscle TMs are shorter than the muscle TMs; sequence analysis demonstrates that each subunit of equine platelet TM consists of 247 amino acids, 37 fewer than for skeletal muscle TM. The major differences in sequence between platelet and skeletal muscle TM are found near the amino and carboxyl terminal ends of the proteins. Probably as the result of such alterations, the non-muscle TMs aggregate in a linear end-to-end manner much more weakly than do the muscle TMs. Since end-to-end interactions are responsible for the highly cooperative manner in which TM binds to actin, the non-muscle TMs have a lower affinity for actin filaments than do the muscle TMs. However, the attachment of other proteins to actin (e.g. the Tn-I subunit of skeletal muscle troponin or the S-1 subfragment of skeletal muscle myosin) can increase the affinity of actin filaments for non-muscle TM. The non-muscle TMs interact functionally with the Tn-I component of skeletal muscle troponin to inhibit the ATPase activity of muscle actomyosin and with whole troponin to regulate the muscle actomyosin ATPase in a Ca++-dependent manner, even though one of the binding sites for troponin on skeletal TM is missing in non-muscle TM. A novel actomyosin regulatory system can be produced using Tn-I, calmodulin and non-muscle TM; in this case inhibition is released when the non-muscle TM detaches from the actin filament in the presence of Ca++. Although it has not yet been demonstrated that the non-muscle TMs participate in a Ca++-dependent contractile regulatory system in vivo it does appear that they are associated with actin filaments in vivo.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000032:analysis; D001923:Brain Chemistry; D002417:Cattle; D002645:Chickens; D005347:Fibroblasts / Q000032:analysis; D006736:Horses; D006801:Humans; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis; D011487:Protein Conformation; D011817:Rabbits; D013045:Species Specificity; D013552:Swine; D013961:Thyroid Gland / Q000032:analysis; D014018:Tissue Distribution; D014335:Tropomyosin / Q000032:analysis*; D014336:Troponin / Q000032:analysis
D000596:Amino Acids; D014335:Tropomyosin; D014336:Troponin