Donor origin of the in vitro hematopoietic microenvironment after marrow transplantation in mice.
Bone marrow stroma from radiochimeric mice was established in culture. The polymorphic enzyme glucose phosphate isomerase (GPI) was used to determine the proportions of donor and recipient present in the original bone marrow and in cultured stroma. Bone marrow initially containing 95% donor GPI, when cultured and subsequently passaged for up to 8 weeks remained about 70% donor GPI. We conclude that many cultured stromal cells are donor derived in our radiochimeras and these are probably of hematopoietic origin.
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D002678:Chimera; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006410:Hematopoiesis; D051379:Mice
D005956:Glucose-6-Phosphate Isomerase