Methacholine-stimulated release of benzoyl-arginine-ethylester esterase from mouse submandibular gland cells.
The possible existence of cholinoceptor for inducing benzoyl-arginine-ethylester (BAEe) esterase release in the mouse submandibular gland was investigated using dispersed-cell preparations from normal male and female, castrated male and testosterone-treated female mice. Methacholine (a cholinoceptive agonist) induced esterase release only in normal female and castrated male mice, both of which had low esterase activity in the submandibular glands, whereas norepinephrine (an alpha-adrenoceptor agonist) induced esterase release in all of the mice. Methacholine-induced esterase release was completely inhibited by atropine, a cholinoceptive antagonist, but not by phenoxybenzamine, an alpha-adrenoceptor antagonist. The effects of metacholine and norepinephrine on esterase release were additive. The results suggest the coexistence of cholinoceptor and alpha-adrenoceptors for esterase release in the mouse submandibular gland. Calcium was essential for esterase release induced by either methacholine or norepinephrine.
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002369:Castration; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D008297:Male; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D013363:Submandibular Gland / Q000187:drug effects / Q000201:enzymology*; D013739:Testosterone / Q000494:pharmacology
D008688:Methacholine Compounds; D010643:Phenoxybenzamine; D016210:Methacholine Chloride; D013739:Testosterone; D001285:Atropine; D002265:Carboxylic Ester Hydrolases; C019763:benzoylarginine ethyl esterase; D002118:Calcium; D009638:Norepinephrine