Cloning of lymphocytes from whole blood by limiting dilution.
A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.
D002470:Cell Survival / Q000528:radiation effects; D002999:Clone Cells / Q000276:immunology; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D007158:Immunologic Techniques; D008213:Lymphocyte Activation / Q000528:radiation effects; D008214:Lymphocytes / Q000276:immunology* / Q000528:radiation effects