Isolated rat hepatocytes as a tool for the determination of antibodies against human liver cell membrane.
Antibodies against liver cell membrane are measured by counting the percentages of fluorescent rat hepatocytes ( FRH ), obtained by an indirect immunofluorescence method after incubation of isolated rat hepatocytes with sera of patients with chronic liver diseases. A close relationship exists between the percentages of FRH and the serological and histological parameters of diseases activity, there was no difference between HBsAg-positive or -negative sera.
D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006521:Hepatitis, Chronic / Q000276:immunology; D006801:Humans; D008099:Liver / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D008103:Liver Cirrhosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D051381:Rats
D001323:Autoantibodies