Injury to cultured endothelial cells from human umbilical vein by linoleic acid hydroperoxide.
The effect of linoleic acid hydroperoxide on cultured endothelial cells from human umbilical vein was examined morphologically. Incubation of the cells with a low concentration (0.5 nmol/ml) of linoleic acid hydroperoxide for 3 h caused a slight decrease in electron density of the mitochondrial matrix. Upon an increase of the concentration to 1.0 nmol/ml, the damage became more pronounced, and enlargement and dilatation of the rough-surfaced endoplasmic reticulum were observed. At higher concentrations (5 and 7.5 nmol/ml), dilatation of the rough-surfaced endoplasmic reticulum became further pronounced, and many vacuoles were observed in the cells.
D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D008854:Microscopy, Electron; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology* / Q000187:drug effects
D008041:Linoleic Acids; D008054:Lipid Peroxides; C008285:linoleic acid hydroperoxide