title: Measurement of serum theophylline concentrations using a modified Ames TDA system.
abstract: Results are presented which show that the Ames TDA Theophylline kit provides an accurate and reliable method of measuring the concentration of theophylline in plasma. It may be used with a shortened incubation time (5 min) without loss of accuracy, and a single calibration curve can be used over a 3-week period. There is an acceptable precision at all assayed levels (coefficient of variation, 3.3%) and results obtained for patients' samples correlate well with those obtained using liquid chromatography (y = 0.02 + 1.06x, r = 0.99) and enzyme immunoassay (y = 0.58 + 1.03x, r = 0.99).
mesh_terms: D001663:Bilirubin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000097:blood; D011933:Reagent Kits, Diagnostic; D013806:Theophylline / Q000097:blood*
chemical_list: D006454:Hemoglobins; D008055:Lipids; D011933:Reagent Kits, Diagnostic; D013806:Theophylline; D001663:Bilirubin