Excretion of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 26-tetrol and corticosterone metabolites in rat bile. Studies on the role of alcohol dehydrogenase in steroid metabolism.
The monosulphate of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 26-tetrol was identified in bile of female and male rats. The excretion in the female rats decreased from 10-20 nmol/h directly after insertion of the bile cannula to very low values after 6 h. It then increased to reach a constant rate after 24 h that was slightly lower than during the first hour. Administration of 4-methylpyrazole or ethanol failed to change the rate of excretion. Thus there was no increase in the rate of excretion that might have resulted from an inhibited oxidation catalyzed by alcohol dehydrogenase (EC 1.1.1.1). Male rats were fed liquid ethanol-containing and control diets for 24 days prior to insertion of the bile cannula. The excretion during the first 3 h was 10-30 nmol/h in both groups, and chronic ethanol treatment did not change this rate. The effect of 4-methylpyrazole on the ratio between the excretion rates of the 3 beta- and 3 alpha-isomers of 3,11 beta, 21-trihydroxy-5 alpha-pregnan-20-one disulphate was measured by a gas chromatographic-mass spectrometric method. The absence of any significant change indicated that the 3 beta-hydroxysteroid dehydrogenase activity of alcohol dehydrogenase was of no significance in the metabolism of corticosterone.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000032:analysis*; D002777:Cholestanols / Q000378:metabolism*; D002849:Chromatography, Gas; D003345:Corticosterone / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013256:Steroids / Q000378:metabolism
D002777:Cholestanols; D013256:Steroids; C010556:cholestane-3,7,12,25-tetrol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D003345:Corticosterone