[An immunoenzyme technic for demonstrating the molecular hybridization of nucleic acids].
An immunoenzymatic procedure has been developed based on the use of monoclonal antibodies specific for 5-bromodeoxyuridine (BdUr). It allows the detection of BdUr-labelled DNA immobilized on a nitrocellulose filter. Using this procedure, it was possible to detect up to 0.5 pg of mammalian DNA labelled in vivo with BdUr, 5 pg of nick-translated BdUr-labelled PBR-322 and, using this latter probe and dot-blot hybridization, 50 pg of native unlabelled PBR-322.
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology / Q000378:metabolism; D003101:Collodion; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D007124:Immunoenzyme Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D010209:Paper; D051381:Rats
D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial; D003101:Collodion; D004247:DNA; D001973:Bromodeoxyuridine
Une technique immunoenzymatique pour la mise en évidence de l'hybridation moléculaire entre acides nucléiques.