Pemphigus in the dog: comparison of immunofluorescence and immunoperoxidase method to demonstrate intercellular immunoglobulins in the epidermis.
In 3 dogs with pemphigus vulgaris and 4 dogs with pemphigus foliaceus, intercellular immunoglobulins were demonstrated in the epidermal stratum spinosum. The immunofluorescence technique on cold ethanol-fixed and paraffin-embedded tissue sections was compared with the immunoperoxidase method on formalin-fixed paraffin-embedded tissue sections. The results of both methods were identical. However, the advantage of the unlabeled antibody-enzyme method was that the same formalin-fixed tissue specimens could be used for conventional light microscopy, as well as for immunohistologic studies.
D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004817:Epidermis; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D010392:Pemphigus / Q000175:diagnosis / Q000662:veterinary*; D012867:Skin / Q000276:immunology*
D007136:Immunoglobulins