title: A study of the fibrin plate assay of fibrinolytic agents. Optimal conditions, reproducibility and precision.
abstract: The reproducibility, precision and required conditions of the fibrin plate method for determination of fibrinolytic agents are described in detail. Under optimal conditions the assay is a sensitive and precise method for the quantitative determination of fibrinolytic agents. Thus, with a tissue plasminogen activator preparation its sensitivity is approximately 0.02 CTA urokinase units/ml, and dilutions yielded a curve with a slope (double-logarithmic) with a day-to-day mean of 0.53 with a coefficient of variation of 5.1%.
mesh_terms: D001780:Blood Coagulation Tests / Q000592:standards; D005069:Evaluation Studies as Topic; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000032:analysis*; D010447:Peptide Hydrolases / Q000378:metabolism; D010960:Plasminogen Activators / Q000032:analysis; D012712:Serum Globulins / Q000032:analysis; D013696:Temperature; D013997:Time Factors
chemical_list: D005343:Fibrinolytic Agents; D012712:Serum Globulins; D010447:Peptide Hydrolases; D010960:Plasminogen Activators