Insulin binding to erythrocytes before and after changing from porcine to biosynthetic human insulin in children with type-I diabetes.
The binding of [125I]insulin to isolated erythrocytes from diabetic children (n = 27) before (group a) as well as one and five months after changing from porcine to biosynthetic human insulin (groups b and c) was investigated. An analysis of variance of the binding parameters, determined by a nonlinear regression procedure, yielded statistically significant differences between the receptor affinities Ka as well as between the receptor concentrations Xo of the groups a and b, a and c and b and c. (formula: see text). The results suggest that the change from porcine insulin to biosynthetic human insulin induces a short-term as well as long-term increase in the affinity, and a decrease in the concentration of the erythrocyte insulin receptors.
D000293:Adolescent; D000818:Animals; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives* / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D007700:Kinetics; D008297:Male; D011972:Receptor, Insulin / Q000378:metabolism*; D013552:Swine
D007328:Insulin; C008147:insulin, iodo-; D011972:Receptor, Insulin