Blood alcohol analysis: a comparison of the gas-chromatographic assay with an enzymatic assay.
Ethanol in blood was assayed by 2 currently available procedures: a commercial kit enzymatic assay and a gas chromatographic procedure. Both methods were performed with commercially available materials and equipment. Results by the 2 procedures agreed well. For the 192 specimens studied, the correlation coefficient was 0.983, and the least-squares values of slope and intercept were 0.999 and 0.001 g/100 ml respectively. Use of isopropanol or ethanol swabs did not affect results of the enzymatic procedure.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000097:blood*; D000435:Alcoholic Intoxication / Q000175:diagnosis* / Q000201:enzymology; D002849:Chromatography, Gas; D000431:Ethanol / Q000097:blood*; D006801:Humans; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D000431:Ethanol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase