Quantitative measurements of an intrastrain cross-reactive idiotype in IgE antibodies.
This paper describes the development of methods for quantitation of an intrastrain cross-reactive idiotype (CRIA) in IgE antibodies directed to the p-azophenylarsonate (Ar) group and their application to normally immunized and idiotypically suppressed mice. The data were validated by the use of monoclonal and serum anti-Ar antibodies known to possess or lack CRIA. The idiotype was absent in the IgE as well as the total anti-Ar population of idiotypically suppressed mice. A good correlation was noted between the CRIA content of total and IgE anti-Ar with a trend toward a somewhat lower content in the IgE population. Irradiated mice that received immune cells prior to further immunization expressed relatively low concentrations of the idiotype (per weight unit of anti-Ar). The method will be useful in studies of the regulation of the switch to IgE synthesis.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007163:Immunosorbent Techniques; D051379:Mice; D011817:Rabbits; D010132:p-Azobenzenearsonate / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E; D010132:p-Azobenzenearsonate