title: Ethanol, an efficient coolant for rapid freezing of biological material.
abstract: Supercooled ethanol was used for freeze-quenching protozoan samples and gave reasonable ultrastructural preservation. The use of ethanol as a coolant has considerable advantages in terms of its relative safety, high cooling efficiency and low cost when compared with other coolants currently used for freeze-quenching.
mesh_terms: D000818:Animals; D000431:Ethanol; D056890:Eukaryota / Q000648:ultrastructure*; D005407:Flagella / Q000648:ultrastructure*; D005615:Freezing; D006652:Histological Techniques; D008854:Microscopy, Electron / Q000379:methods; D008870:Microtubules / Q000648:ultrastructure
chemical_list: D000431:Ethanol