Identification of aminoglycoside-acetylating enzymes by high-pressure liquid chromatographic determination of their reaction products.
A method to identify the aminoglycoside-acetyltransferase (AAC) enzymes AAC(3), AAC(2') and AAC(6') by high-pressure liquid chromatographic characterization of their products of reaction with tobramycin or sisomicin is described. Conditions are given for the chromatography of kanamycin A, netilmicin, neomycin, and apramycin, and their products of reaction, if any, with the three AAC enzymes are listed.
D000107:Acetylation; D000123:Acetyltransferases / Q000032:analysis*; D000617:Aminoglycosides / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D009764:o-Phthalaldehyde / Q000032:analysis
D000617:Aminoglycosides; D009764:o-Phthalaldehyde; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase