Homogeneous luminescence immunoassay for total estrogens in urine.
We describe an homogeneous luminescence immunoassay for "total" estrogens in enzymically hydrolyzed urine from nonpregnant women. The antiserum, raised against estriol-16,17- dihemisuccinate conjugated to bovine serum albumin, specifically bound the C-19 steroids carrying the estrogen-characteristic phenolic group. 17 beta-Estradiol conjugated with aminobutylethyl isoluminol was used to monitor the immunological reaction; this conjugate was stable for at least two years. Because binding to the antiserum markedly enhances the light-producing efficiency of the tracer, no separation of bound and free antigen is necessary. Results (microgram/24 h) by this method (y) correlated well (r = 0.958) with those by a conventional fluorometric (x) method (y = 2. 51x - 2.83). The sensitivity (detection limit) is 4 micrograms/L and the precision compares well with that of commonly used RIA methods. The method appears suited to large numbers of samples, as in menstrual cycle monitoring.
D003429:Cross Reactions; D004964:Estriol / Q000031:analogs & derivatives / Q000276:immunology; D004967:Estrogens / Q000652:urine*; D005260:Female; D005470:Fluorometry; D006801:Humans; D007106:Immune Sera; D007158:Immunologic Techniques; D008163:Luminescent Measurements; D008598:Menstruation; D008722:Methods
D004967:Estrogens; D007106:Immune Sera; C009341:estriol succinate; D004964:Estriol