Improved determination of cytochrome b5 in human erythrocytes.
A sensitive, precise enzymic/spectrophotometric method for determining cytochrome b5 in small amounts of blood is described. Mean values for healthy individuals, ages 20 to 70 years, were 0.26 (SD 0.03) mumol per liter of erythrocytes or 0.87 (SD 0.14) nmol per gram of hemoglobin. We believe the assay is preferable to methods described hitherto, primarily because of its high sensitivity.
D000328:Adult; D000368:Aged; D002021:Buffers; D003579:Cytochrome Reductases; D003573:Cytochrome b Group / Q000097:blood*; D042966:Cytochrome-B(5) Reductase; D015786:Cytochromes b5; D004912:Erythrocytes / Q000201:enzymology*; D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008706:Methemoglobin; D008875:Middle Aged; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D012016:Reference Values; D013053:Spectrophotometry; D013382:Subtraction Technique; D013696:Temperature
D002021:Buffers; D003573:Cytochrome b Group; D009243:NAD; D008706:Methemoglobin; D015786:Cytochromes b5; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase