[Mechanisms of production of tumor necrosis factor (TNF)--reconstitution experiment white nude mice].
In order to investigate the role of T cells in the production of tumor necrosis factor (TNF), a reconstitution experiment was performed with nude mice (Balb/c, nu/nu). The results obtained were as follows: The cytotoxic activity of tumor necrosis serum (TNS) from Balb/c, nu/nu mice treated with P. acnes-LPS was 1/22 against that from Balb/c, nu/+ mice. TNF activity increased 14 times in reconstituted nude mice against Balb/c, nu/nu mice. Investigation of the production of the cytotoxic activity per cell was carried out using T cell and macrophage fractions separated from the spleens of both Balb/c, nu/nu and Balb/c, nu/+ mice treated with P. acnes as a priming agent. Elicitation employing LPS was done in vitro. Cytotoxic activity released into culture medium was observed in the macrophage fraction, but not in the T cell fraction. However, no significant difference was shown in species. With P. acnes treatment, the population of macrophages in the spleens from Balb/c, nu/+ mice increased 25.5 times, whereas that from Balb/c, nu/nu mice only increased 6.8 times. The above results suggest that the mechanism of the incremental effect of T cells on TNF production was due to the promotion of macrophage proliferation during the priming period after injection of P. acnes.
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004926:Escherichia coli / Q000276:immunology; D006023:Glycoproteins / Q000096:biosynthesis*; D006131:Growth Inhibitors / Q000096:biosynthesis*; D007958:Leukocyte Count; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011425:Propionibacterium acnes / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D006131:Growth Inhibitors; D014409:Tumor Necrosis Factor-alpha