title: Two immunologically different penicillin amidases synthetized by Escherichia coli PCM 271.
abstract: From Escherichia coli PCM 271 cells two penicillin amidases were separated by affinity chromatography on immunoadsorbent column. In cells grown in organic medium the activities of the amidase 1 and 2 were 30 and 70% respectively, whereas the activity of the amidase 1 in the cells grown in inorganic medium increased up to 98%. The amidase 1 migrated faster in polyacrylamide gel electrophoresis and was retained on DEAE-cellulose in 10 mM phosphate buffer, pH 8. No catalytic differences were demonstrated between the amidases.
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D007120:Immunochemistry; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000581:Amidohydrolases; D010399:Penicillin Amidase