In vitro dehalogenation of para-substituted aromatic halides in rat liver preparations.
The in vitro dehalogenation of a series of para-substituted halobenzenes was studied using HPLC separation followed by scintillation counting or neutron-activation analysis. Microsomal and cytosolic deiodination were established for iodobenzene substrates whose para-substituents were CO2H, CHO, NO2, OH, and C6H5 but not for para-iodobenzonitrile. A nonglutathione cytosolic deiodinase was only indicated with 4-iodobiphenyl as the substrate. In vitro dehalogenation could not be established for 4-bromobiphenyl using neutron-activation analysis.
D000818:Animals; D002713:Chlorine / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes / Q000378:metabolism; D007462:Iodobenzenes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009501:Neutron Activation Analysis; D011865:Radioisotope Dilution Technique; D011868:Radioisotopes / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D011868:Radioisotopes; D002713:Chlorine; D005982:Glutathione Transferase; D005978:Glutathione