Transaminase inhibitors block glycolysis and G1 to S phase progression in chick embryo fibroblasts: reversal by alpha-keto acids.
Two transaminase inhibitors, aminooxyacetate and cycloserine, inhibited the initiation of insulin-stimulated DNA synthesis in chick embryo fibroblasts. This inhibition was overcome when pyruvate (4 mM), oxaloacetate (4 mM), or alpha-ketobutyrate (10 mM) was included in the culture medium with hormone and inhibitor. Aminooxyacetate also inhibited lactate production in insulin-treated cultures in the absence of added alpha-keto acid.
D000085:Acetates / Q000494:pharmacology*; D000625:Aminooxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002642:Chick Embryo; D003523:Cycloserine / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects*; D007651:Keto Acids / Q000494:pharmacology*; D010071:Oxaloacetates / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D000637:Transaminases / Q000037:antagonists & inhibitors*
D000085:Acetates; D002087:Butyrates; D007328:Insulin; D007651:Keto Acids; D010071:Oxaloacetates; D011773:Pyruvates; D000625:Aminooxyacetic Acid; C005087:alpha-ketobutyric acid; D019289:Pyruvic Acid; D003523:Cycloserine; D000637:Transaminases