Does the DNA methylase Eco dam pair nucleotide sequences to form site-specific duplexes?
The Eco dam methylase is active on denatured DNA and single-stranded synthetic oligonucleotides containing GATC sites. The results suggest that on interaction with single-stranded oligonucleotides the Eco dam methylase is able to form a duplex structure within the GATC site, and that this duplex site is a substrate for enzyme.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008780:Methyltransferases / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013379:Substrate Specificity; D013950:Thymus Gland
D009838:Oligodeoxyribonucleotides; D004247:DNA; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)