A rapid method for the detection and quantitation of IgA protease activity by macrobore gel-permeation chromatography.
A rapid assay to detect and quantitate immunoglobulin A1 (IgA1) protease activity was developed by the use of a high-performance gel-permeation chromatography column. The assay measured the disappearance of intact substrate and the emergence of cleavage fragments and the results could be expressed in absolute units. The utility of the assay was demonstrated in the partial purification of an IgA1 protease from a strain of Haemophilus influenzae.
D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006193:Haemophilus influenzae / Q000201:enzymology; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis*; D012697:Serine Endopeptidases; D013379:Substrate Specificity
D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase