On the interaction between synthetic detergents and enzymatic proteins.
Eight highly purified and crystalline enzymes representative of fundamental metabolic pathways and of cellular structure were tested with seven detergents. The enzyme-detergent interaction was studied as a time-independent (inhibition) or time-dependent (inactivation) phenomenon. Our results imply a specificity of detergent action on the enzymes studied.
D000410:Alanine Transaminase / Q000378:metabolism; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003902:Detergents / Q000494:pharmacology*; D004798:Enzymes / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D004798:Enzymes; D013501:Surface-Active Agents; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000410:Alanine Transaminase; D008049:Lipase; D000469:Alkaline Phosphatase