title: Plasmid RP4 encodes two forms of a DNA primase.
abstract: The pri gene locus of the conjugative broad host range plasmid RP4 maps between coordinates 40.3 and 43.5 and encodes two antigenically related forms of a DNA primase with a molecular mass of 118 and 80 kDa (kilodalton). Genesis of these two products has been examined using Pri+-recombinant plasmids. As shown by deletion analysis, the primase polypeptides are tow separate translation products which arise from an in-phase overlapping gene arrangement. It is suggested that transcription of a set of RP4 genes including the pri gene starts at a promoter site within the Tra1 region. In vivo, RP4 mutant primase can apparently substitute for Escherichia coli primase as demonstrated by measuring suppression of the dnaG3 (ts) mutant.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D019915:DNA Primase; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D011815:R Factors; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases