Mutagenicity studies on herring gulls from different locations on the Great Lakes. II. Mutagenic evaluation of extracts of herring-gull eggs in a battery of in vitro mammalian and microbial tests.
Herring-gull (Larus argentatus) eggs were collected from five locations on the Great Lakes and from one colony on the Atlantic coast for organochlorine analysis and mutagenesis testing. The Great Lakes colonies were chosen for their different contaminant levels, while the Atlantic coast colony was used as a relatively clean control. The eggs were homogenized and extracted, and the extracts were tested in the Salmonella/mammalian microsome assay for induction of point mutations and in Chinese hamster ovary (CHO) cells for the induction of sister chromatid exchanges (SCEs) and chromosome aberrations. None of the extracts was mutagenic in Salmonella, either in the presence or absence of metabolic activation. However, all of the extracts, including the clean control, caused significant increases in both the SCE rate and in the number of chromosome aberrations in the CHO cells. There was no apparent relationship between contaminant levels and the magnitude of these responses or the doses at which they occurred, although the chemical analysis indicated a wide range in the concentrations of the different organochlorides present.
D000818:Animals; D001717:Birds / Q000196:embryology* / Q000235:genetics; D002869:Chromosome Aberrations / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004531:Eggs; D004785:Environmental Pollutants / Q000633:toxicity*; D006114:Great Lakes Region; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014020:Tissue Extracts / Q000633:toxicity
D004785:Environmental Pollutants; D009153:Mutagens; D014020:Tissue Extracts