Monoclonal antibodies to surface antigens of rabbit type II pneumocytes.
Techniques for the production of monoclonal antibodies to cell surface antigens of type II pneumocytes are reported. Using these techniques, over 200 hybridomas were produced from spleen cell fusions of 8 mice. Of these, 25 expressed activity toward the type II pneumocyte cell surface. Many antibodies cross-reacted with a variety of cells and membranes from other organs and species, suggesting that these antibodies were ubiquitous to membrane antigens. Most of the antibodies cross-reacted strongly with dog lung membranes, suggesting the existence of common mammalian lung determinants. Five hybridomas produced supernatant antibody with considerable specificity for type II pneumocytes. Ascites fluid antibody was produced to these 5 hybridomas. Immunofluorescent staining of type II pneumocyte cell surfaces could be demonstrated with all 5 of these antibodies. This initial study demonstrates the feasibility of producing monoclonal antibodies to cell surface antigens of the type II pneumocyte.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D009928:Organ Specificity; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000276:immunology; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014422:Turkeys
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface