Calcium ions protect cell-substratum adhesion receptors against proteolysis. Evidence from immunoabsorption and electroblotting studies.
An antibody preparation against BHK cell wheat germ agglutinin receptors (anti-WGA-R), which inhibits fibronectin-mediated cell adhesion, was characterized by immunoabsorption and electroblotting experiments. The results indicated that a 48 kD cell surface WGA-R receptor is protected from proteolysis by Ca2+ ions and is important in fibronectin-mediated cell adhesion.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion; D006224:Cricetinae; D005353:Fibronectins / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D010447:Peptide Hydrolases / Q000494:pharmacology*; D011975:Receptors, Mitogen / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D005353:Fibronectins; D011975:Receptors, Mitogen; C027503:wheat germ agglutinin receptor; D010447:Peptide Hydrolases; D002118:Calcium