Antigenic profile of some typical and septate phialide-strains of Aspergillus fumigatus.
Antigens from typical and septate phialide-strains of Aspergillus fumigatus, A. fumigatus var. ellipticus, A. fischeri, A. flavus and A. niger were analysed by fused rocket and two-dimensional immunoelectrophoresis. Depsite strain-to-strain variations, isolates of the two morphological forms of A. fumigatus showed a close similarity in their antigenic profile; the percentage of qualitative sharing ranged between 80% and 100%. The number of antigens shared by the isolates of A. fumigatus var. ellipticus and A. fischeri as compared with a typical A. fumigatus isolate was relatively high. By contrast isolates of A. flavus and A. niger, which are taxonomically not so closely related to A. fumigatus, revealed a qualitative sharing of only 16% and 8%, respectively. The number of common antigenically active components identified in culture filtrate antigens from the typical and septate phialide-strains of A. fumigatus was around 29. Two-dimensional electrophoresis with tandem and intermediate gel proved further that the two types of A. fumigatus isolates are antigenically homologous. Present findings also support the view that the antigenically active components of this pathogen are predominantly protein-glycoprotein in nature.
D000946:Antigens, Fungal / Q000032:analysis* / Q000302:isolation & purification; D001230:Aspergillus / Q000145:classification / Q000276:immunology; D001232:Aspergillus fumigatus / Q000145:classification / Q000276:immunology*; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D012703:Serotyping / Q000379:methods
D000946:Antigens, Fungal