Influence of cations on the mode of action of miconazole on yeast cells.
The influence of the antifungal agent miconazole nitrate on yeast plasma membranes was studied in a concentration range 0-100 microM. The reaction of 100 microM miconazole with the plasma membranes lead to a rapid breakdown of the transmembrane pH gradient and to an efflux of metabolites from the cytoplasm of the cells. This effect of miconazole could be reversed by mono-, di- and most effectively by trivalent cations due to the formation of miconazole-cation complexes. At a ratio of trivalent cation/miconazole (1:3) the effect was completely reversed. X-Ray diffraction studies indicated a crystalline structure of the aluminium-miconazole complex.
D000535:Aluminum; D001692:Biological Transport / Q000187:drug effects; D002412:Cations / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology; D011232:Chemical Precipitation; D006863:Hydrogen-Ion Concentration; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D008825:Miconazole / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D014961:X-Ray Diffraction
D002412:Cations; D007042:Hypoxanthines; D019271:Hypoxanthine; D008825:Miconazole; D000535:Aluminum