title: Construction and partial characterization of recombinant cDNA clones for chicken type I collagen messenger RNAs.
abstract: Messenger RNAs for the alpha 1 and alpha 2 chains of type I procollagen were partially purified from total embryonic chicken calvaria using gel chromatography on Sepharose 4B and used to construct recombinant cDNA clones corresponding to both mRNAs. Restriction site mapping, nucleotide sequencing and hybridization to RNA blots were used to show that clones pCAL1 and pCAL2 contain inserted sequences corresponding to the mRNAs for chicken alpha 1 and alpha 2 procollagen chains, respectively.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011347:Procollagen / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D014169:Transformation, Bacterial
chemical_list: D004274:DNA, Recombinant; D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen