A biotin-avidin technique for the localisation of membrane-bound monoclonal antibodies by low power transmission electron microscopy.
A biotin-avidin horseradish peroxidase system is described for the localisation of membrane-bound monoclonal antibodies by low power transmission electron microscopy. An indirect bridge technique was used, with a biotin-labelled goat anti-mouse immunoglobulin antibody and horseradish peroxidase-conjugated avidin D. The technique provided preparations of high morphological and ultrastructural quality which facilitated accurate cellular identification. Positive immunostaining was specific and precisely defined.
D000911:Antibodies, Monoclonal / Q000032:analysis*; D001360:Avidin; D001666:Binding Sites, Antibody; D001710:Biotin; D002462:Cell Membrane / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000032:analysis / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000032:analysis / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin