Plaque-forming cell response of frozen human lymphocytes stimulated with pokeweed mitogen. II. Results obtained in cultures of irradiated T and untreated B lymphocytes.
The PFC capability of cryopreserved lymphocytes was tested after T/B cell separation and irradiation of the T cells. It is concluded that there is no selective loss of function of the involved subpopulations. Thus nitrogen-stored lymphocytes can safely be used in PFC assays after conventional separation and irradiation procedures.
D000293:Adolescent; D000328:Adult; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D005260:Female; D005615:Freezing; D006462:Hemolytic Plaque Technique; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology / Q000528:radiation effects
D011043:Pokeweed Mitogens