A monoclonal antibody raised against adult Schistosoma mansoni which recognizes a surface antigen on schistosomula.
A monoclonal antibody has been raised by immunizing a mouse with an isolated tegumental preparation of adult Schistosoma mansoni. The hybridoma designated NIMP/M.47, secreted an IgG2a antibody which was positive by indirect immunofluorescence with live schistosomula of S. mansoni, but not with live schistosomula of S. bovis, or with other living life cycle stages of S. mansoni. In complement-dependent, or cell-mediated in vitro cytotoxicity assays, the monoclonal antibody mediated levels of schistosomular killing as high as those obtained with sera from infected mice. No significant protection, however, was obtained in passive transfer experiments. NIMP/M47 was specific for a 20,000 dalton polypeptide in the schistosomular surface, which was also recognized by serum from infected mice.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology*; D007116:Immunization, Passive; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface