Expression of the rpsO and pnp genes: structural analysis of a DNA fragment carrying their control regions.
Precise physical mapping of the genes rpsO and pnp coding respectively for ribosomal protein S15 and polynucleotide phosphorylase together with regions involved in the regulation of their expression has been obtained by the analysis of in vitro deletion mutants. The results suggest that each gene has its own promotor, but that there is coexpression of rpsO and pnp. The nucleotide sequence of rpsO and of the beginning of pnp is presented and includes the presumed regulatory regions of these genes. Several features of the sequence support the mapping experiments and are discussed in relation to the expression of the ribosomal and pnp genes.
D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D009876:Operon; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D014158:Transcription, Genetic
D012269:Ribosomal Proteins; D011117:Polyribonucleotide Nucleotidyltransferase