Radioimmunoassay of gastrin--our experience and a review of current status.
The procedures of radioimmunoassays for gastrin are presented in this paper. Techniques are described and evaluated with special reference to preparation of radioiodinated gastrin, purification of monoiodinated gastrin, characterisation of the immunochemical properties of radiolabelled gastrin, raising and characterisation of specific antibodies, incubation conditions, separation of bound and free hormone and treatment of data. Factors contributing to discrepancies of radioimmunoassay results and problems encountered are discussed. Methods employed by the authors in gastrin radioimmunoassay are described. Commercially available gastrin kits are also compared.
D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004381:Duodenal Ulcer / Q000175:diagnosis; D005755:Gastrins / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007457:Iodine Radioisotopes; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012633:Secretin; D013552:Swine; D015043:Zollinger-Ellison Syndrome / Q000175:diagnosis
D005755:Gastrins; D007457:Iodine Radioisotopes; D011933:Reagent Kits, Diagnostic; D012633:Secretin