[Determination of the hepatitis B virus surface antigen (HBsAg) by immunoenzyme analysis].
Studies aimed at the development of a variant of ELISA for the determination of hepatitis B virus surface antigen (HBsAg) showed the assay to be most effective when a modified periodate method of conjugation of highly purified horseradish peroxidase with the IgG-fraction of antiserum to HBsAg was used. With the resulting conjugates, the "sandwich"-test on polystyrene solid phase could detect HBsAg in concentrations up to 5 ng/ml which is several thousand-fold higher in sensitivity than counter immunoelectrophoresis, one order of magnitude more sensitive than the passive hemagglutination test, and comparable with the radioimmunoassay (RIA). The specificity of the method was confirmed by positive results of the neutralization test. By this method, HBsAg in the sera of hepatitis B patients was detected as frequently as by the RIA.
D003378:Counterimmunoelectrophoresis; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D011233:Precipitin Tests; D011863:Radioimmunoassay
D006514:Hepatitis B Surface Antigens; D006735:Horseradish Peroxidase
Opredelenie poverkhnostnogo antigena virusa gepatita B (HBsAg) metodom immunofermentnogo analiza.