18B1: an axon-specific antigen associated with many proteins.
We describe an antigen, 18B1, defined by a monoclonal antibody. Immunoperoxidase staining of brain sections shows that 18B1 is selectively associated with neurofilament-rich axons. Antibody staining of sodium dodecyl sulphate-gel blots, on the other hand, shows that 18B1 is associated with a large number of proteins, none of which are structural components of brain neurofilaments. The antigen is sensitive to a variety of proteases but is not degraded by various glycosidases, suggesting that 18B1 is probably an amino acid sequence. Its association with neurofilament-rich axons together with its absence from neurofilaments themselves suggests that it may be involved in mediating interactions between neurofilaments and the proteins that bear it.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D001369:Axons / Q000032:analysis*; D002525:Cerebellar Cortex / Q000166:cytology; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D011689:Purkinje Cells / Q000166:cytology; D051381:Rats
D000911:Antibodies, Monoclonal; D000941:Antigens