A cloning strategy for the bacteriophage T4 vs gene.
The product of the bacteriophage T4 vs gene, the tau peptide, has been shown to thermally stabilize temperature-sensitive valyl-tRNA synthetase (EC 6.1.1.9). To clone the bacteriophage T4 vs gene, recombinant pBR322-T4 DNA molecules were used to transform Escherichia coli CP 790302 (valSts). Transformants that grew at 43.5 degrees C on selective medium exhibited some properties indicative of a phage-modified valyl-tRNA synthetase. The data are consistent with successful cloning of the vs gene and atypical modification of the valyl-tRNA synthetase in CP 790302.
D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005814:Genes, Viral; D006358:Hot Temperature; D013604:T-Phages / Q000235:genetics*; D014161:Transduction, Genetic; D014162:Transfection; D014508:Urea / Q000494:pharmacology; D014633:Valine / Q000378:metabolism; D014637:Valine-tRNA Ligase / Q000378:metabolism
D004274:DNA, Recombinant; D014508:Urea; D014637:Valine-tRNA Ligase; D014633:Valine