Correlative genotoxicity studies of airborne particles in Salmonella typhimurium and cultured human lymphocytes.
The acetone extracts of ambient air particulates collected locally were tested for their capacity to induce sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) in human lymphocytes, and to induce gene mutations (GMs) in Salmonella typhimurium. The extracts caused dose-related clastogenic/mutagenic responses in all three assay systems. With the same concentration, it seems that the Ames Salmonella/microsomal assay with TA98 gave the highest, and the chromosomal aberration assay with human lymphocytes the lowest, mutagenic/clastogenic responses, respectively. Because high frequencies of SCEs were induced by solvent extracts of airborne particles, this study further indicated the usefulness of SCE assay in human lymphocytes for genotoxicity studies of airborne particles.
D000393:Air Pollutants / Q000633:toxicity*; D002869:Chromosome Aberrations; D003434:Crossing Over, Genetic; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects; D012854:Sister Chromatid Exchange
D000393:Air Pollutants; D009153:Mutagens