Rapid immunoassays for the acrosome reaction of sea urchin sperm utilizing antibody to bindin.
Two assays employing rhodamine-conjugated and peroxidase-conjugated antibindin are presented. The assays are used to detect the occurrence of the acrosome reaction in sea urchin sperm. The assays are rapid and can be performed on large numbers of samples. The scoring of the reacted and unreacted cells can be done accurately at a rate of 60 cells/min.
D000177:Acrosome / Q000032:analysis / Q000502:physiology*; D000818:Animals; D005089:Exocytosis; D005260:Female; D005306:Fertilization; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D008297:Male; D008858:Microscopy, Phase-Contrast; D011956:Receptors, Cell Surface; D012235:Rhodamines; D012617:Sea Urchins; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*
D006023:Glycoproteins; D011956:Receptors, Cell Surface; D012235:Rhodamines; C035027:egg surface sperm receptor; D006735:Horseradish Peroxidase