[Advantages of using swine serum in culturing animal and human cell lines].
Using the culture of lymphoid human cells, mice myeloma cells and hybridoma (the latter was obtained by fusion of myeloma cells with those of mice spleen immunized with phage lambda), the fetal serum was shown to be surpassed in growth activity by that from adult swines. This fact is especially pronounced at small serum concentrations. When hybridoma cells were cultured there were no differences in titre of monoclonal antibodies specific to phage lambda with the use of both sera. The possibility of substitution of swine serum for human one was demonstrated using the culture of lymphocytes from human peripheral blood.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000494:pharmacology; D003584:Cytological Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D008940:Mitotic Index / Q000187:drug effects; D013552:Swine / Q000276:immunology*
D003470:Culture Media; D007106:Immune Sera
O preimushchestvakh ispol'zovaniia svinoĭ syvorotki pri kul'tivirovanii nekotorykh liniĭ kletok zhivotnykh i cheloveka.