title: Immunochemical and immunohistochemical studies on the specificity of a monoclonal antibody to choline acetyltransferase of rat brain.
abstract: Choline acetyltransferase (ChAT) preparations partially purified from brains of rat, mouse and cat were electrophoresed in polyacrylamide gels and blotted on nitrocellulose sheets. A monoclonal antibody, Ab-57, bound specifically to one protein band which coincided with the peak of ChAT activity in each species. Using Ab-57, ChAT was demonstrated immunohistochemically in cholinergic structures in rat, mouse and cat brains. Thus, the specificity and cross-species reactivity of the monoclonal antibody Ab-57 have been established.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D001921:Brain / Q000201:enzymology*; D002415:Cats; D002795:Choline O-Acetyltransferase / Q000276:immunology*; D002799:Cholinergic Fibers / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D051379:Mice; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase