Quantitative evaluation of anti-HBs in sera.
The Authors carried out a methodological study about the way of expressing in mIU/ml the antibody to HBV surface antigen (anti-HBs) titer. The results showed that Hollinger's formula seems to provide a good accuracy when enzyme immunoassay is employed (AUSAB EIA, Abbott). Moreover, moderate variations in the commercial anti-HBs kits studied (AUSAB, AUSAB EIA, Abbott) have been verified. Considering that and the progress of the calibration curve of the WHO Reference International Standard, it is also advised to include always in the test a calibration curve of the standard serum when a higher precision is required.
D000914:Antibodies, Viral / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008433:Mathematics; D008722:Methods; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic