Analysis of cell populations in crescentic glomerulonephritis.
The cell types present in the crescents were studied in 5 human patients with crescentic glomerulonephritis: two cases of systemic lupus erythematosus, one case of hemolytic uremic syndrome and two cases of rapidly progressive glomerulonephritis. Frozen sections of renal biopsies were studied by immunofluorescence, using murine monoclonal antibodies (orthoclones) against specific antigens on the membrane of human peripheral blood cells, and by histochemical methods. Monocytes (OKM1+, OKIa+ cells) but no lymphocytes (OKT+ cells), were detected in the crescentic glomeruli. Subsets of T lymphocytes (inducer-helper and cytotoxic-suppressor) were detected in the interstitium. Non-specific esterase-positive cells were observed in the glomeruli and in small numbers in the crescents. Fibrinogen deposits were present in the crescents of four of the five cases studied. No immunoglobulins (IgG, IgM, IgA) or complement (C1q, C3) deposits were detected in the crescents. Fibrinogen, immunoglobulins and complement were present in the glomerular tufts.
D000911:Antibodies, Monoclonal; D004950:Esterases / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D005921:Glomerulonephritis / Q000276:immunology / Q000473:pathology*; D006801:Humans; D008264:Macrophages / Q000201:enzymology / Q000473:pathology*; D009000:Monocytes / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal; D004950:Esterases