Elastolysis of insoluble elastin.
We developed an assay for measurement of elastolytic activity using insoluble 3H-labelled particulate elastin adherent to plastic that is capable of detecting 150 picograms of pancreatic elastase. This equals or exceeds the sensitivity of the most sensitive previously reported systems, without requiring sodium dodecyl sulfate treatment of the elastin. Elastin digestion is dependent upon substrate and elastase concentration, but is not linearly related to time. This is partially attributable to elastase denaturation or autolysis under the assay conditions. The assay could easily detect elastase secreted by either peritoneal or alveolar macrophages. Compared to previously described assays using substrates that closely resemble the physiologic substrate, this represents a considerable increase of sensitivity of detection of elastolytic activity of enzymes.
D000818:Animals; D002417:Cattle; D004548:Elasticity; D004549:Elastin; D010450:Endopeptidases / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism*; D011817:Rabbits; D012995:Solubility; D013552:Swine; D014316:Tritium
D014316:Tritium; D004549:Elastin; D010450:Endopeptidases; D010196:Pancreatic Elastase