1H NMR methods for the noninvasive study of metabolism and other processes involving small molecules in intact erythrocytes.
1H NMR methods are described with which resolved resonances can be obtained for many of the small molecules in intact erythrocytes. In one method, the more intense hemoglobin resonances are suppressed by transfer of saturation throughout the hemoglobin spin system by cross relaxation following a selective saturation pulse. In a second method, the hemoglobin resonances are eliminated with the spin-echo pulse sequence by using a between-pulse delay time long enough for complete elimination of the hemoglobin resonances by spin-spin relaxation. Selected examples of the study of erythrocyte biochemistry by 1H NMR are discussed.
D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009243:NAD / Q000097:blood
D006454:Hemoglobins; D009243:NAD; D006859:Hydrogen; D005978:Glutathione