Identification of the bacteriophage T4 unf ( = alc) gene product, a protein involved in the shutoff of host transcription.
The introduction of plasmid pR386 into E. coli cells renders them restrictive to the growth of phage T4 unf ( = alc) mutants. This system has been used to isolate Unf+ revertants, which, along with the mutant parental strains, have been used to identify the unf gene product by two-dimensional gel electrophoresis. Synthesis of the unf gene product, a polypeptide of just over 18,000 daltons in size, begins within 1 min after infection and terminates at about 12 min after infection at 30 degrees. Gene dosage experiments suggest that the unf protein functions catalytically.
D004269:DNA, Bacterial / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D007525:Isoelectric Focusing; D013604:T-Phages / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D014764:Viral Proteins