Quantitative histochemistry of the angiotensinase A (APA) in the renal glomeruli of rats after stimulation of the renin-angiotensin system.
Quantitative histochemical measurements of aminopeptidase A (APA, angiotensinase A) were done kinetically in the kidney glomeruli of rats after short-term experiments (treatment with furosemide as well as captopril for 2, 4, and 6 h). The APA activities increased after treatment with furosemide or captopril. Highest activities were determined after 4 h using furosemide and 6 h using captopril. It is concluded that glomerular APA activities correspond to the renin/angiotensin plasma levels and that the fast changes of APA activities are well demonstrable by kinetic densitometric measurements in situ.
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D002216:Captopril / Q000494:pharmacology*; D005665:Furosemide / Q000494:pharmacology*; D043384:Glutamyl Aminopeptidase; D006651:Histocytochemistry; D007678:Kidney Glomerulus / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D011392:Proline / Q000031:analogs & derivatives*; D051381:Rats; D011919:Rats, Inbred Strains; D012084:Renin-Angiotensin System / Q000187:drug effects*
D005665:Furosemide; D011392:Proline; D002216:Captopril; D000626:Aminopeptidases; D043384:Glutamyl Aminopeptidase