Purification of thiogalactoside transacetylase by affinity chromatography.
Thiogalactoside transacetylase, the product of the lacA gene of the lactose operon of Escherichia coli, has been purified by an improved procedure. The enzyme binds tightly to immobilized Cibacron Blue F3GA columns and can be eluted by potassium chloride in high concentrations. Final purification was obtained by affinity chromatography on an agarose-coenzyme A column followed by gel filtration.
D000123:Acetyltransferases / Q000235:genetics / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000201:enzymology; D005796:Genes; D014227:Triazines
D014227:Triazines; C012842:Cibacron Blue F 3GA; D000123:Acetyltransferases; C022770:galactoside acetyltransferase