title: Functional limits of the araIc promoter suggest an additional regulatory site for araBAD expression.
abstract: The araBAD promoter is defined, in part, by two types of cis-acting constitutive mutations, araIc at position -35 and araXc at position -10. Subcloning experiments demonstrated that the araIc and araIcXc promoters require DNA sequence information out to position -53 to -56 for maximum constitutive expression. This is 8 to 10 base pairs more DNA than is generally thought to be necessary for RNA polymerase interaction. The -53 to -56 region is required for glucose repression, suggesting that an additional factor interacts in this region and is necessary for maximum expression.
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005809:Genes, Regulator; D005947:Glucose / Q000494:pharmacology; D009154:Mutation; D009876:Operon; D011953:Receptors, Cyclic AMP / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics
chemical_list: D011953:Receptors, Cyclic AMP; D001089:Arabinose; D012321:DNA-Directed RNA Polymerases; D005947:Glucose