Haplodiploid gene expression in maize and its detection.
A method for the demonstration of the gametophytic origin of genetic variability in maize is described. For genes coding dimeric or multimeric enzymes, haploid expression can be demonstrated by means of translocations between A and B chromosomes (TB-A), which make it possible to obtain hyperploid pollen grains, partially diploid and heterozygous for electrophoretic mobility. Comparison of the electrophoretic pattern of this pollen type (three bands) and that of normal grains produced by a heterozygous F/S plant (two bands only) reveals haploid transcription of the monomeric forms. The procedure was tested on ADH-1 and used to demonstrate haploid expression for GOT-1. The data obtained suggest, moreover, that the reduction in male gamete transmission of duplications may be due to differences in pollen competitive ability rather than to processes affecting microspore maturation.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D001219:Aspartate Aminotransferases / Q000235:genetics; D003433:Crosses, Genetic; D004171:Diploidy; D014644:Genetic Variation; D006238:Haploidy; D006579:Heterozygote; D010944:Plants / Q000235:genetics*; D014178:Translocation, Genetic; D003313:Zea mays / Q000235:genetics
D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D001219:Aspartate Aminotransferases