title: Monoclonal antibodies to the major protein of the murine zona pellucida: effects on fertilization and early development.
abstract: The growing murine oocyte is surrounded by an extracellular zona pellucida consisting of three sulfated glycoproteins, ZP-1, ZP-2, and ZP-3. The smallest of these, ZP-3, has been reported to be the species-specific sperm receptor. Monoclonal antibodies have been recently characterized to three different antigenic determinants, two found exclusively on ZP-2, and one found on both ZP-2 and ZP-3. The in vivo effect of these antibodies on the three known functions of the zona pellucida were examined. The most dramatic effect was the prevention of fertilization. After administration, the monoclonal antibodies were located in the ovary on the zona pellucida of growing oocytes. Eggs ovulated subsequently were coated with the monoclonal antibodies and failed to develop into 2-cell embryos after mating. Eighty days later, the monoclonal antibodies could no longer be detected on the zona of ovarian oocytes, and this loss coincided with the resumption of fertility. These findings provide molecular evidence for the hypothesis that the immunological block to sperm-egg binding need not involve antibody specific for the sperm receptor, and that antibodies to the zona pellucida block sperm access by steric hinderance. Other known functions of the zona were unaffected. The antibodies were unable to induce the biochemical changes in the zona associated with the postfertilization block to polyspermy and had no detectable effect on preimplantation development.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis*; D001755:Blastocyst / Q000502:physiology; D002455:Cell Division; D004527:Egg Proteins; D005260:Female; D005306:Fertilization; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D008562:Membrane Glycoproteins; D051379:Mice; D008819:Mice, Nude; D010053:Ovary / Q000502:physiology; D010063:Ovum / Q000502:physiology*; D011956:Receptors, Cell Surface; D015044:Zona Pellucida / Q000502:physiology*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D004527:Egg Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606519:Zp1 protein, mouse; C000606520:Zp2 protein, mouse; C000606521:Zp3 protein, mouse