Polyamine oxidase-mediated trypanosome killing: the role of hydrogen peroxide and aldehydes.
Trypanosoma lewisi and T. musculi were lysed when incubated with bovine serum in the presence of either spermine or spermidine. Similar results were obtained when a fraction from bovine serum containing polyamine oxidase (PAO) activity or a commercially available purified beef plasma PAO were used in lieu of bovine serum. Trypanosomes treated with cytotoxic concentrations of PAO-spermine failed to establish infection in rats. These results are similar to those from our previous studies with African trypanosomes. We now extend the properties of PAO by showing that human retroplacental serum (RPS) containing PAO activity was also capable of mediating trypanosome killing. This is of significance because the macrophage PAO resembles the human RPS PAO. In addition, our preliminary studies, in which an attempt was made to characterize the factors responsible for cytotoxicity, suggested that a number of products of the PAO-polyamine reaction display trypanocidal properties. These included hydrogen peroxide (H2O2), the aldehyde acrolein, and possibly aminoaldehydes. No evidence was obtained that the oxygen intermediates, superoxide and hydroxyl radicals, play a role in the PAO-mediated trypanosome killing. Ammonia, an additional product of PAO-polyamine reaction, was not trypanocidal. Furthermore, the data suggested that less than 30 min exposure to the reaction mixture (and possibly to aminoaldehydes) was adequate to cause irreversible damage to trypanosomes.
D000171:Acrolein / Q000494:pharmacology*; D000447:Aldehydes / Q000494:pharmacology*; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D005260:Female; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood*; D010100:Oxygen / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D011073:Polyamines / Q000097:blood; D011247:Pregnancy; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology; D014350:Trypanosoma lewisi / Q000187:drug effects / Q000254:growth & development*; D000097667:Polyamine Oxidase
D000171:Acrolein; D000447:Aldehydes; D000641:Ammonia; D002374:Catalase; D005609:Free Radicals; D006861:Hydrogen Peroxide; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010100:Oxygen; D011073:Polyamines; D013482:Superoxide Dismutase; D000097667:Polyamine Oxidase