title: Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella.
abstract: Non-flagellate H2 mutants were isolated from a phase-2 stable strain, SJW806 H1-gt- H2-enxon vh2-, a derivative of Salmonella typhimurium. By transductional crosses a deletion map and a recombination map of the H2 gene were made. There are three regions especially rich in nonflagellate mutational sites. By the use of the deletion map, mutational sites of 21 flagellar shape mutants were also determined. Most of them were located at two regions which coincide with two of the three regions rich in non-flagellate mutational sites. A gene, vh2, is closely linked to the promoter side of the H2 gene. Three-factor transductional crosses showed that the vh2 gene was on the left of the H2 gene in the present map. The H2 gene forms part of an operon with the distal gene rh1 which specifies the H1 repressor. Thus, a polarity effect of the H2 mutations on the expression of the rh1 gene was examined by observing whether a wild-type H1 allele introduced into the H2 mutants was expressed or not. Many of the H2 mutations were polar, and most of the strongly polar mutations were located in the left (promoter-proximal) half of the H2 gene, while most of the mutations in the right half of the gene were weakly polar or non-polar.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D005407:Flagella / Q000032:analysis; D005408:Flagellin / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D009876:Operon; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin