Does glucose affect phospholipase A2 activity in pancreatic islets?
Rat pancreatic islet homogenates catalyzed the production of labelled oleate and lysophosphatidylcholine from 1-palmitoyl-2-[1-14C] oleoylphosphatidylcholine and di[1-14C] stearoylphosphatidylcholine, respectively. The phospholipase A2 was activated by Ca2+ (Ka approximately 0.5 mM) and inhibited by mepacrine. No difference in enzymic activity was found in glucose-deprived and glucose-stimulated islets, respectively.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002250:Carbon Radioisotopes; D005947:Glucose / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000201:enzymology*; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats
D002250:Carbon Radioisotopes; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D005947:Glucose; D002118:Calcium