Large scale purification and structural properties of yeast aspartyl-tRNA synthetase.
A large scale purification procedure of baker's yeast aspartyl-tRNA synthetase is described which yields more than 200 mg pure protein starting from 30 Kg of wet commercial cells. The synthetase is an alpha 2 dimer of Mr = 125,000 +/- 5,000 which can be crystallized (J. Mol. Biol. 138, 1980, 129-135). The enzyme has an elongated shape with a Stokes radius of 50 A and a frictional ratio of 1.5. The synthetase has a tendency to aggregate but methods are described where this effect is overcome.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D001226:Aspartate-tRNA Ligase / Q000302:isolation & purification* / Q000378:metabolism; D003460:Crystallization; D046911:Macromolecular Substances; D008970:Molecular Weight; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D046911:Macromolecular Substances; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase