The accuracy of poly(U) translation by different eukaryotic tRNAs.
In this work we present comparative data on rates of phenylalanine and leucine incorporation into the poly(U) dependent product of cell-free translation by different eukaryotic tRNAs at high Mg2+ concentration. The frequency of translation errors has been found to depend upon the value of the tRNAPhe:tRNALeu ratio and the peculiarities of isoacceptor tRNAsLeu of different origin.
D000818:Animals; D002250:Carbon Radioisotopes; D002454:Cell Differentiation; D005260:Female; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011072:Poly U / Q000235:genetics*; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013045:Species Specificity; D014908:Triticum / Q000378:metabolism
D002250:Carbon Radioisotopes; D011072:Poly U; D010649:Phenylalanine; D012343:RNA, Transfer; D007930:Leucine