A monoclonal anti-DNA antibody also binds to cell-surface protein(s).
A murine monoclonal anti-DNA antibody ( PME77 ) has been found to bind tightly to the plasma membrane of Raji cells. We show here that this monoclonal anti-DNA antibody reacts in a radioimmunoassay with the cell surface of a variety of mammalian cell types and that the antigenic determinant recognized by the monoclonal anti-DNA antibody at the surface of Raji cells is resistant to DNase. It belongs to polypeptides removed from the cell surface by a mild proteinase K treatment.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004247:DNA / Q000276:immunology*; D019286:Endopeptidase K; D010450:Endopeptidases / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008565:Membrane Proteins; D004247:DNA; D010450:Endopeptidases; D019286:Endopeptidase K