Disappearance of enkephalins in the isolated perfused rat lung.
Enkephalin disappearance during a single passage through the isolated, Krebs'-perfused rat lung was examined by superfusion bioassay. The rat colon was used to quantitate enkephalin disappearance since it proved to be sensitive to physiologic concentrations (10(-11) M) of met5-enkephalin or an analog D-ala2-D-leu5-enkephalin. The rat stomach strip was used to assess the release of prostaglandins from the pulmonary vasculature. The rat lung rapidly degraded the enkephalins but released no prostaglandins in the dose-range of 0.1 - 50 ng. Captopril at doses which blocked conversion of angiotensin I to II inhibited the degradation of enkephalins across the lung.
D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002216:Captopril / Q000494:pharmacology; D003106:Colon / Q000187:drug effects; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine; D004744:Enkephalin, Methionine / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D010477:Perfusion; D011453:Prostaglandins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000187:drug effects
D004745:Enkephalins; D011453:Prostaglandins; D000804:Angiotensin II; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; D000803:Angiotensin I; D002216:Captopril; D007213:Indomethacin