Improvements to the plaque assay for antibody secreting cells.
A capillary glass microslide has been adapted to function as a chamber for the determination of plaque forming cell responses. Comparisons with Cunningham chambers indicate no significant difference between methods. Further, microslides arrive clean and ready to use, thus eliminating the need to assemble chambers as necessitated by the Cunningham method. Used in conjunction with an electronically assisted enumerating device, the microslides provide a rapid and less tedious means for assaying large numbers of animals for PFC responses.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001322:Autoanalysis; D002452:Cell Count; D006462:Hemolytic Plaque Technique / Q000295:instrumentation; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology