An ATP-stimulated factor that enhances the nuclear binding of "activated" receptor-glucocorticoid complex.
A macromolecular material that enhances the translocation, or binding, of already "activated" receptor-glucocorticoid complex to nuclei in the presence of 5 mM ATP was separated from the cytosol of rat liver by DEAE-cellulose column chromatography with about 0.025 M NaCl. The molecular weight of the material was about 93,000 +/- 4,900, as determined by agarose gel filtration. After incubation at 60 degrees C for 15 min, this material still had activity to increase the nuclear binding, but on boiling for 15 min it lost its activity.
D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000502:physiology*; D006358:Hot Temperature; D066298:In Vitro Techniques; D007201:Indicator Dilution Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D000255:Adenosine Triphosphate