Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae.
We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards Bg/II (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004260:DNA Repair / Q000528:radiation effects; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins