Expression of H-2Db on the cell surface in the absence of detectable beta 2 microglobulin.
In this report we describe a variant of the C57BL/6 T lymphoma EL4 (EL4/Mar) which, in contrast to the parental cell line, expresses neither H-2Kb nor beta2-microglobulin (beta2m) but which does express H-2Db detectable by serology and by alloreactive cytotoxic T lymphocytes (CTL). This observation raises the possibility that H-2Db and perhaps other major histocompatibility complex class I molecules are normally not associated with beta2m on the cell surface. In addition, this report is the first to indicated that alloreactive CTL can interact with a beta2m-free class I antigen.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D011232:Chemical Precipitation; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000032:analysis* / Q000276:immunology; D064532:Histocompatibility Antigen H-2D; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology / Q000502:physiology*
D000954:Antigens, Surface; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D001613:beta 2-Microglobulin