Enumeration of Treponema pallidum cells cultivated in vitro by an enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay was developed to enumerate Treponema pallidum cells. The assay could detect from 2 X 10(7) to 4 X 10(8) treponemes per ml. Reactive rabbit serum and goat anti-rabbit immunoglobulin G (peroxidase conjugate) were used in the assay. Optimum results were obtained when 2,2'-azino-di(ethylbenzthiazolinesulfonic acid) was used as the dye for the enzyme reaction and the reactions were allowed to run for 45 min. Interestingly, assays in which in vivo-cultivated T. pallidum was used produced lower absorbance values than those in which T. pallidum was cultivated in vitro.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005404:Fixatives; D006041:Goats / Q000276:immunology; D007124:Immunoenzyme Techniques; D008828:Microbiological Techniques; D011817:Rabbits / Q000276:immunology; D014210:Treponema pallidum / Q000166:cytology* / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D005404:Fixatives; D014357:Trypsin