title: Islet cell cytoplasmic and surface antibodies' prevalence and beta cell specificity of islet cell surface antibodies in some subjects with putative islet cell autoimmunity.
abstract: In human sera two methods of islet cell cytoplasmic antibody (ICA) detection (on Bouin-fixed or cryostate sections of human pancreas) and the islet cell surface antibody (ICSA) method on isolated rat islet cells were compared. The ICA detection on Bouin-fixed sections was more sensitive than that on cryostate sections. In two groups of subjects with putative islet cell autoimmunity ICA prevalence was higher than ICSA prevalence. However, a weak correlation between both types of antibodies was established. In one group of healthy subjects with cytotoxic serum against rat beta cells a high prevalence of ICSA was found, whereas none of them had ICA. Defined on the basis of a positive immunofluorescence on rat islet cells, ICSA positive sera of newly diagnosed IDDM patients reacted to a significantly higher percentage with beta cells than did normal controls. In conclusion, of all methods compared ICA detection on Bouin-fixed pancreas sections seems to be the most suitable method for the detection of an islet cell autoimmunity.
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D000293:Adolescent; D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology; D002648:Child; D003593:Cytoplasm / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006801:Humans; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000276:immunology*; D010851:Picrates; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000085:Acetates; D001323:Autoantibodies; D010851:Picrates; D005557:Formaldehyde; C026239:Bouin's solution; D019342:Acetic Acid