[Comparison of bacteriological and immunoenzyme methods for the serum determination of sisomicin. Pharmacokinetic application in the human].
A modification of the serum gentamicin immunoenzymatic method (Syva- Biom  erieux kit) for sisomicin assay is proposed and compared to the bacteriological reference method. It shows a higher sensitivity (0.1 mg.l-1) with a shortened time of analysis (less than 1 hour) and a good reproducibility (range: 4.7% to 1.6% for concentrations between 0.5 mg.l-1 and 10 mg.l-1) but the concentrations are 15 p. cent lower as compared to the bacteriological method. A pharmacokinetic study, performed in five patients, enables us to demonstrate the praticability of this method for the adaptation of the dosage regimen in renal insufficiency.
D001681:Biological Assay; D004334:Drug Administration Schedule; D004754:Enterobacter / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D007273:Injections, Intramuscular; D007700:Kinetics; D012853:Sisomicin / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism
D012853:Sisomicin
Comparaison des méthodes bactériologique et immuno-enzymatique pour le dosage sérique de la sisomicine. Application pharmacocinétique chez l'homme.