Comparative studies of Trypanosoma cruzi and T. cruzi-like stocks from different South American countries using lectins.
The agglutination behaviour of four-day-old epimastigote culture forms of 34 Trypanosoma cruzi, and T. cruzi-like stocks from Brazil, Chile, Colombia, Ecuador and Peru were tested with 15 carbohydrate-specific lectins. We distinguished intraspecifically two groups of agglutination reactions: Group 1 includes stocks which react with Triticum vulgaris and Aaptos papillata II (wheat germ agglutinin: WGA-type). Group 2 includes stocks agglutinated by Arachis hypogaea and Aaptos papillata II (peanut lectin: PNA-type). The agglutination reactions with lectins from Triticum vulgaris and Aaptos papillata II correlate with the presence of N-acetylneuraminic acid on the cell surface. After treatment with neuraminidase, the WGA-type is agglutinated by PNA but not by lectins from Triticum vulgaris and Aaptos papillata II. Further results demonstrate that a certain zymodeme pattern can be correlated with carbohydrate determinants.
D000372:Agglutination Tests; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D037102:Lectins; D009439:Neuraminidase / Q000494:pharmacology; D019887:Peanut Agglutinin; D013020:South America; D014345:Trypanosoma / Q000032:analysis / Q000145:classification*; D014349:Trypanosoma cruzi / Q000032:analysis / Q000145:classification*; D014909:Wheat Germ Agglutinins
D002241:Carbohydrates; D037102:Lectins; D019887:Peanut Agglutinin; D014909:Wheat Germ Agglutinins; D009439:Neuraminidase