A new rapid semi-quantitative enzyme immunoassay suitable for determining immunity to tetanus.
A sensitive enzyme immunoassay for rapidly assessing a patient's state of immunity to tetanus is described. The test, which uses 50 microliter sample of blood, plasma or serum, is done in a capillary tube and, by comparison with two adjacent reference tubes containing standardised sera, places immunity to tetanus in one of three categories--low-negative (less than 0.01 IU/ml), intermediate (0.01-1.28 IU/ml) or high (greater than 1.28 IU/ml). In a study of 90 clinical specimens assayed both by toxin neutralisation bioassay and capillary enzyme immunoassay the enzyme immunoassay accurately assessed the state of immunity to tetanus of the patients concerned.
D000907:Antibodies, Bacterial / Q000032:analysis*; D001681:Biological Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D013742:Tetanus / Q000276:immunology*
D000907:Antibodies, Bacterial