[Primary structure of the E. coli DNA-dependent RNA-polymerase beta'-subunit. Hydrolysis with trypsin].
The carboxymethylated beta'-subunit of DNA-dependent RNA polymerase was hydrolyzed with trypsin. The hydrolysate was separated on Bio-Gel P-4, followed by ion exchange chromatography, and was further purified by paper chromatography and electrophoresis. A mixture of large peptides was digested with Staphylococcus aureus protease, the fragments obtained were separated by an HPLC procedure. As a result, 172 peptides were isolated, the complete amino acid sequence for 162 and partial sequence for 10 of them being determined. In total, these peptides contain 862 amino acid residues.
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D010455:Peptides / Q000032:analysis; D014357:Trypsin
D010455:Peptides; D012321:DNA-Directed RNA Polymerases; D014357:Trypsin
Pervichnaia struktura beta'-sub''edinitsy DNK-zavisomoĭ RNK-polimerazy E. coli. Gidroliz tripsinom.