Coupling between phosphatidylinositol metabolism and cdc 28 gene product of Saccharomyces cerevisiae. On the possible mechanism of cdc 28 gene action.
It was shown that the decrease in phosphatidylinositol (PI) content in cdc 28 G1-cells was due to a defect in inositol transport. This decrease in inositol transport was linked to microtubular function which was evident by the effect of a microtubular disrupting agent (colcemid) on inositol transport in stationary phase A364A cells. The involvement of PI in yeast G1 phase was further substantiated by the observation that o-phenanthroline, which blocks yeast cells in G1 phase, could inhibit inositol transport and PI levels as well. It is proposed that the regulation of PI metabolism is mediated by the gene cdc 28 and that microtubules may play a major role in the mechanism of action of this gene product.
D003703:Demecolcine / Q000494:pharmacology; D007399:Interphase; D008870:Microtubules / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D010716:Phosphatidylinositols; D003703:Demecolcine