Mitochondrial DNA-binding proteins that bind preferentially to supercoiled molecules containing the D-loop region of Xenopus laevis mtDNA.
From the bulk of the Xenopus laevis mitochondrial proteins insoluble in 1% Triton X-100 + 1M NaCl, we have isolated, by DNA-cellulose chromatography, a protein fraction enriched in DNA-binding proteins. This fraction contains proteins showing a specific affinity for supercoiled DNA molecules containing the mitochondrial DNA displacement-loop region, as measured by filter binding and competition assays.
D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant; D004278:DNA, Superhelical / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D010053:Ovary / Q000378:metabolism; D010957:Plasmids; D014981:Xenopus
D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins