[Relation between PBPs (penicillin-binding proteins) and growth curves of Escherichia coli submitted to ampicillin and oxacillin].
Growth curves recorded by the MS-2 (high performance photometer) provide a new means for studying PBPs. Indeed, the increase in prelytic OD (observed during the first two hours of contact between beta- lactams and cultures) is concentration- independent for antibiotics bound to PBPs3 ( Yourassowsky et al., 1981). Oxacillin has high affinity for PBP3 of E. coli (Curtis et al., 1979). Growth curves confirm biochemical studies: prelytic increase of OD of E. coli cultures is concentration- independent for oxacillin (100-2000 micrograms/ml), conversely to results observed with ampicillin and amoxycillin.
D000667:Ampicillin / Q000494:pharmacology*; D001426:Bacterial Proteins; D002268:Carboxypeptidases / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D010068:Oxacillin / Q000494:pharmacology*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D000667:Ampicillin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002268:Carboxypeptidases; D002267:Muramoylpentapeptide Carboxypeptidase; D010068:Oxacillin
Relation entre les PBPs et les courbes de croissance d'Escherichia coli soumis á l'ampicilline et á l'oxacilline.