title: Comparison of apheresis and other methods for separation and purification of hemopoietic stem cells: initial experience with a blood buffy coat model for the use of autologous bone marrow transplantation.
abstract: With an increasing number of bone marrow transplantations (BMT) being contemplated in leukemia and cancer patients, it is prudent for blood banks to develop a suitable program within their resources for harvesting, purifying and freezing bone marrow stem cells. In order to do this, initially a prototype has been developed involving buffy coat model (BC) using normal donor blood. Centrifugation, sedimentation and machine apheresis methods were separately evaluated leading to a combined and sequential handling procedure. Blood was passed through a cell separator resulting collection of BC with 90% reduction of the volume showing 80% recovery of total leucocytes and 87% yield of mononuclear cells. Following centrifugation the cells with DMSO were frozen in a controlled freezing system and stored in liquid nitrogen. After thawing 94% cells were recovered with 93% viability. The initial experience gained in the model system could be incorporated in autologous BMT program in patients but requires modifications for improved results; the latter will be described separately.
mesh_terms: D001773:Blood Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008747:Methylcellulose
chemical_list: D008747:Methylcellulose