Fc receptors on granulocytes from patients with rheumatoid arthritis and Felty's syndrome.
Receptors for the Fc part of IgG on polymorphonuclear cells (FcR) of patients with rheumatoid arthritis (RA), Felty's syndrome (FS) and healthy controls (HC) were studied by means of a rosetting technique with rabbit IgG coated ox erythrocytes. When cold isolated polymorphonuclear cells (PMN) were incubated at room temperature the percentage of rosette forming PMN (RF-PMN) from HC was more than twice that measured directly after isolation at 4 degrees C. The same phenomenon was observed for PMN from patients with RA although the RF-PMN increased by only 1/3. In contrast warming of PMN from patients with FS did not influence the RF-PMN. The presence of surface bound immunoglobulins and intracytoplasmic immunoglobulins was measured by immunofluorescence and appeared to be inversely related to the RF-PMN. A good correlation between the results of the C1q binding assay (C1qBA) and the immunofluorescence score was observed but no correlation existed between the C1qBA and the RF-PMN. These results indicate that the number of PMN expressing FcR in patients with RA and FS is decreased presumably because of the phagocytosis of immune complex like material. The decrease in the availability of FcR may influence the functions of the PMN.
D000936:Antigen-Antibody Complex / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D003593:Cytoplasm / Q000276:immunology; D005258:Felty Syndrome / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D009504:Neutrophils / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis*; D012397:Rosette Formation; D013696:Temperature
D000936:Antigen-Antibody Complex; D007136:Immunoglobulins; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D015922:Complement C1q; D003166:Complement Activating Enzymes