Advantage of monoclonal vs. polyclonal antisera in a beta 2-microglobulin radioimmunoassay.
Unassociated human beta 2-microglobulin (beta 2m) and histocompatibility peptide associated beta 2-microglobulin produce significantly different inhibition curves in a competitive binding assay with rabbit anti-beta 2-microglobulin. This difference disallows the use of the rabbit antisera for measuring MHC-beta 2-microglobulin complex concentration using free beta 2m as a standard. Presumably the close amino acid sequence homology between human and rabbit beta 2-microglobulin results in rabbit antisera against a small number of epitopes, some of which are covered or conformationally altered in the MHC-beta 2-microglobulin complex. A monoclonal anti-beta 2-microglobulin antibody, LG1 reacted equally well with both forms of beta 2-microglobulin and is used to measure the concentration of beta 2-microglobulin in the histocompatibility peptide complex.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D007106:Immune Sera; D001613:beta 2-Microglobulin