Stimulatory and inhibitory effects of adrenaline and 8-bromo-cAMP on insulin-sensitive 2-deoxyglucose transport in rat adipocytes.
The effects of adrenaline and 8-bromo-cAMP on 2-deoxyglucose uptake in isolated rat adipocytes were investigated under conditions of unidirectional flux, in which the transport process is rate limiting. Adrenaline showed a dualistic effect in the absence of insulin. At concentrations below 1 microM adrenaline stimulated 2-deoxyglucose uptake; at higher concentrations adrenaline inhibited the uptake. In the presence of insulin at the maximum effective concentration, addition of adrenaline further increased the 2-deoxyglucose uptake by about 50%. In the presence of insulin 8-bromo-cAMP had the same effect as adrenaline. In the absence of insulin, 8-bromo-cAMP only inhibited 2-deoxyglucose uptake.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D003837:Deoxy Sugars; D007328:Insulin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003847:Deoxyglucose; D004837:Epinephrine