title: Granulated folliculo-stellate cells and growth hormone cells immunostained with anti-S 100 protein serum in the pituitary glands of the goat.
abstract: Goat pituitary glands were immunohistochemically studied with antisera for bovine S-100 protein, rat LH beta, FSH, TSH beta, prolactin, ovine GH, and porcine ACTH1-39 by use of the superimposition technique on adjacent sections. Folliculo-stellate (F-S) cells were divided into two categories on the basis of ultrastructural properties: One consisted of a mass of agranular cells in which the pseudolumina were equipped with microvilli and cilia. Elongate gap junctions were often observed among these cells. The other was a group of granulated cells with or without pseudolumina. In this group the gap junctions were shown to be disintegrated. The dense granules 150-250 nm in diameter began to accumulate in the cells. However, neither type of these F-S cells was immunostained for S-100 protein. On the other hand, numerous polygonal, elongate, irregular or stellate cells containing S-100 protein were distributed throughout the gland. Most of them were immunohistochemically identical with the GH cells laden with the secretory granules 250-450 nm in diameter, but some of them were identical to TSH and prolactin cells which immunostained faintly for S-100 protein. This appears to be the first demonstration of GH cells intensely immunostained for S-100 protein.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D006041:Goats / Q000033:anatomy & histology*; D013006:Growth Hormone / Q000378:metabolism; D007158:Immunologic Techniques; D008297:Male; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000166:cytology* / Q000648:ultrastructure; D011388:Prolactin / Q000378:metabolism; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism
chemical_list: D009418:S100 Proteins; D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone