Electrophoretic analyses of alcohol dehydrogenase, aldehyde dehydrogenase, aldehyde reductase, aldehyde oxidase and xanthine oxidase from horse tissues.
Cellulose acetate zymograms of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (AHD), aldehyde reductase (AHR), aldehyde oxidase (AOX) and xanthine oxidase (XOX) extracted from horse tissues were examined. Five ADH isozymes were resolved: three corresponded to the previously reported class I ADHs (EE, ES and SS) (Theorell, 1969); a single form of class II ADH (designated ADH-C2) and of class III ADH (designated ADH-B2) were also observed. The latter isozyme was widely distributed in horse tissues whereas the other enzymes were found predominantly in liver. Four AHD isozymes were differentially distributed in subcellular preparations of horse liver: AHD-1 (large granules); AHD-3 (small granules); and AHD-2, AHD-4 (cytoplasm). AHD-1 was more widely distributed among the horse tissues examined. Liver represented the major source of activity for most AHDs. A single additional form of NADPH-dependent AHR activity (identified as hexonate dehydrogenase), other than the ADHs previously described, was observed in horse liver. Single forms of AOX and XOX were observed in horse tissue extracts, with highest activities in liver.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000444:Aldehyde Dehydrogenase; D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D000818:Animals; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006736:Horses / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008297:Male; D013347:Subcellular Fractions / Q000201:enzymology; D014018:Tissue Distribution; D014969:Xanthine Oxidase / Q000032:analysis*
D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C019642:alcohol dehydrogenase (NADP+); D014969:Xanthine Oxidase; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D042931:Aldehyde Oxidase