Immunohistochemistry on semi-thin sections of hydroxypropyl methacrylate embedded tissues.
Hydroxypropyl methacrylate formulations for embedding hard or soft tissues are described. Dehydration, infiltration and embedding are carried out at 0-4 degrees C. Semi-thin serial sections may be stained by various techniques, including immunohistochemical and enzyme histochemical procedures. Lymphoid tissues and the detection of surface antigens may present some special problems, however, and these are discussed.
D000179:Acrylates; D000818:Animals; D004386:Duodenum / Q000166:cytology / Q000276:immunology; D005260:Female; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D008689:Methacrylates; D051379:Mice; D008807:Mice, Inbred BALB C; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D014021:Tissue Preservation
D000179:Acrylates; D005404:Fixatives; D008689:Methacrylates; C032802:hydroxypropyl methacrylate