Quantification of enterochromaffin cells with serotonin immunoreactivity in the duodenal mucosa in coeliac disease.
Enterochromaffin cells in the duodenal mucosa were stained by using a monoclonal antibody against serotonin immunoreactive sites and an indirect immunoperoxidase technique. A semi-automatic image analysing system showed increased numbers of these cells in patients with untreated coeliac disease compared with a control group. The number of serotonin positive granules in individual enterochromaffin cells also seemed to be increased in patients with coeliac disease, a finding which may be related to the pathogenesis of this disorder.
D000293:Adolescent; D000911:Antibodies, Monoclonal / Q000276:immunology; D002446:Celiac Disease / Q000378:metabolism / Q000473:pathology*; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D002838:Chromaffin System / Q000473:pathology*; D004386:Duodenum / Q000032:analysis / Q000473:pathology*; D004759:Enterochromaffin Cells / Q000032:analysis / Q000473:pathology*; D005260:Female; D005983:Glutens; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007413:Intestinal Mucosa / Q000032:analysis / Q000473:pathology*; D008297:Male; D012701:Serotonin / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D012701:Serotonin; D005983:Glutens