title: Contemporary methodology for protein structure determination.
abstract: The techniques used for the characterization of protein and peptide structure have undergone great changes that have improved the speed, reliability, and applicability of the process. High-performance liquid chromatography and gel electrophoresis have made the purification of proteins and peptides a routine procedure, even when the compound of interest is a minor component of a complex biological mixture. The chemistry and instrumentation used in amino acid analysis and amino acid sequencing now permit the analysis of as little as 5 to 50 picomoles of samples. This represents an increase in sensitivity of more than a thousandfold over the last 10 years and has made possible the structural analysis of a wide variety of scarce but important compounds.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D005591:Chemical Fractionation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D010447:Peptide Hydrolases; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D010447:Peptide Hydrolases