[Expression in Escherichia coli cells of mutant human interferon alpha2].
cDNA library was obtained from mRNA isolated from human leukocytes induced by Newcastle disease virus. Clones containing cDNA for alpha 2-interferons were identified by colony hybridization with two synthetic hexadecanucleotides. One of the positive clones contained a NH2-terminal part of cDNA of human interferon identical to cDNA for IFN-alpha 2. The only difference between these two clones was the Ser-8 leads to Asn-8 substitution in deduced sequenced of mature interferons. This mutant interferon, named alpha 2, was expressed in E. coli and its properties were compared with those of interferon alpha 2.
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D009154:Mutation; D009876:Operon; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011863:Radioimmunoassay
D004269:DNA, Bacterial; D007370:Interferon Type I; D012333:RNA, Messenger; D004247:DNA
Ekspressiia v kletkakh Escherichia coli mutantnogo interferona alpha2 cheloveka.