Hepatitis B virus and host cell membrane antigens on a primary hepatocellular carcinoma cell line.
We have defined some membrane antigens of the cultured hepatocellular carcinoma cell line PLC/PRF/5, which contains the integrated genome of hepatitis B virus. Using fluoresceinated antibodies, we identified HLA Class 1 glycoprotein and HB surface antigen on the membrane. Only in a minority of cells was physical association of these antigens demonstrated by co-capping. The presence of a "division" antigen was indicated by reactivity of the cells with the murine monoclonal antibody OKT9.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology*; D015788:HLA-A1 Antigen; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D007152:Immunologic Capping; D008114:Liver Neoplasms, Experimental / Q000276:immunology*
D006680:HLA Antigens; D015788:HLA-A1 Antigen; D006514:Hepatitis B Surface Antigens