Cloning of the Escherichia coli release factor 2 gene.
The protein release factor 2 (RF2) participates in Escherichia coli polypeptide chain termination with codon specificity (UAA or UGA). A colicin E1 recombinant identified in the Carbon and Clarke E. coli bank contains the protein release factor 2 gene. A 1.7-kilobase E. coli fragment has been subcloned into the plasmid pUC9 vector. Bacterial cells, containing the plasmid recombinant, produce elevated levels of protein release factor 2 as detected by an immune precipitation assay and in vitro measurement of UGA-directed peptide chain termination and [3H]UGA codon recognition.
D003086:Bacteriocin Plasmids; D003001:Cloning, Molecular; D003062:Codon; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids
D003062:Codon; D010454:Peptide Termination Factors; C041460:peptide chain termination release factor 2