title: Yeast ribosome core particles deficient in acidic proteins L44 and L45 and their activity in reconstitution experiments.
abstract: The yeast ribosome core particles partly depleted of acidic proteins, L44 and L45, were isolated and their activity was examined using a highly purified protein synthesizing system. It was shown that both the phenylalanine polymerization reaction and the elongation factor 2-dependent GTP hydrolysis were stimulated by the addition of the extracted acidic protein fraction. The results obtained clearly indicate a functional role of these proteins in the elongation step of eukaryotic protein synthesis. The essential parameters of ribosome reconstitution experiments are discussed.
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000378:metabolism; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; C029466:ribosomal protein L2