Enhanced multiplication of intracellular (amastigote) stages of Trypanosoma cruzi in vitro.
Amastigotes of different strains of Trypanosoma cruzi responded to stimulation with concanavalin A in an axenic medium by increased DNA synthesis and cell multiplication. These effects were inhibited by alpha-methyl mannoside. Other mitogens, i.e. phytohemagglutinin P, castor bean ricin Type II isolated from Ricinus communis, and a bacterial lipopolysaccharide, had no effect on amastigote growth. Amastigote stimulation by concanavalin A lends itself to studies on the biochemistry and cell cycle of this human pathogen.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008766:Methylmannosides / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D037121:Plant Lectins; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000378:metabolism
D037102:Lectins; D008070:Lipopolysaccharides; D008766:Methylmannosides; D010835:Phytohemagglutinins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D003208:Concanavalin A; C008466:methylmannoside; D004247:DNA