[Incorporation of plasmid DNA into liposomes from the total lipid of E. coli and determination of their stability].
Large monolamellar liposomes were constructed from the total E. coli lipid by ultrasonication and consecutive treatment with Ca2+ and EDTA. Serum albumin and plasmid DNA were incorporated into the liposomes with the efficiency of 6.3 and 4.7%, respectively. The plasmid DNA remained intact after incorporation, as was demonstrated by gel electrophoresis and transformation of E. coli with the DNA extracted from the liposomes, About one half of DNA-containing liposomes remained undamaged after 10 hr incubation at 4 degrees C. Possible implications of E. coli lipid liposomes in genetic transformation are discussed.
D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000378:metabolism*; D004586:Electrophoresis; D004926:Escherichia coli / Q000235:genetics*; D050356:Lipid Metabolism; D008081:Liposomes / Q000138:chemical synthesis / Q000378:metabolism*; D010957:Plasmids; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D008081:Liposomes; D012710:Serum Albumin, Bovine
Vnedrenie plazmidnoĭ DNK v liposomy iz total'nogo lipida E. coli i opredelenie ikh stabil'nosti.