Identification of 6 alpha- and 7 alpha-hydroxyestrone as major metabolites of estrone and estradiol in porcine uterus.
Polar metabolites extracted from the effluents of viable porcine uterine strips superfused with either 6,7-3H-estrone or 6,7-3H-estradiol were identified as a 1:1 mixture of 6 alpha-hydroxyestrone and 7 alpha-hydroxyestrone by paper chromatography in various systems, derivatization and crystallizations to a constant specific activity. The hydroxylated compounds are the only derivatives detected after estrone superfusion. The major metabolite of estradiol released in short-time experiments is estrone followed by its 6 alpha- and 7 alpha-hydroxylated derivatives.
D000818:Animals; D002854:Chromatography, Paper; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism*; D005260:Female; D006894:Hydroxyestrones / Q000302:isolation & purification*; D011865:Radioisotope Dilution Technique; D013552:Swine; D014316:Tritium; D014599:Uterus / Q000378:metabolism*
D006894:Hydroxyestrones; D014316:Tritium; C040162:6-hydroxyestrone; C040163:7-hydroxyestrone; D004970:Estrone; D004958:Estradiol