Tissue uptake and catabolic studies of 125I SS-B (La) injected into mice.
The radiolabeled soluble cellular antigen 125I SS-B (La) has a plasma half-life of 3 min following iv injection into BALB/C mice. Uptake by Kupffer cells (KC) and proximal renal tubular (PRT) cells was demonstrated by autoradiography (ARG). That trichloracetic acid (TCA)-soluble products of 125I SS-B appeared in plasma within 1 min of iv injection suggests rapid in vivo breakdown. Activated peritoneal macrophages (APM) degraded 125I SS-B in a time- and cell-dose-dependent fashion. These findings suggest that the plasma clearance and catabolism of 125I SS-B may be dependent on its interaction with phagocytic cells. This rapid antigen elimination may protect against harmful autoantibody responses.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000032:analysis / Q000652:urine; D001324:Autoantigens; D001345:Autoradiography; D007457:Iodine Radioisotopes; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007700:Kinetics; D007728:Kupffer Cells / Q000378:metabolism*; D008262:Macrophage Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010447:Peptide Hydrolases / Q000378:metabolism; D010587:Phagocytosis; D012261:Ribonucleoproteins; D014238:Trichloroacetic Acid; D000097585:SS-B Antigen
D000941:Antigens; D001324:Autoantigens; D007457:Iodine Radioisotopes; D010447:Peptide Hydrolases; D012261:Ribonucleoproteins; D014238:Trichloroacetic Acid; D000097585:SS-B Antigen