title: Chenodeoxycholic acid pool size determination from children using isotope dilution mass spectrometry.
abstract: An isotope dilution mass spectrometry method is described for determining chenodeoxycholic acid pool size in children. The stable isotopically labeled tracer, (11,12-2H2) chenodeoxycholic acid, was administered orally to children, and the enrichment of bile was measured by selected ion monitoring gas chromatography mass spectrometry. The level of (11,12-2H2)chenodeoxycholic acid enrichment found in the patient samples was in the range of 0.5 to 5%. Data are presented illustrating the duplication of this method in two independent laboratories using standard quadrupole mass spectrometers. This procedure provides the clinician with a non-radioactive method for determining chenodeoxycholic acid pool size which is especially beneficial in studies involving children and pregnant women.
mesh_terms: D002635:Chenodeoxycholic Acid / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D011865:Radioisotope Dilution Technique
chemical_list: D002635:Chenodeoxycholic Acid