Necessity of polyamines for maximum in vivo synthesis of beta beta' subunits of RNA polymerase.
The possibility that polyamines can stimulate the in vivo synthesis of beta beta' subunits of RNA polymerase has been examined through the use of a polyamine-requiring mutant of Escherichia coli. Results from autoradiographic estimation and activity measurement of RNA polymerase in cell extracts prepared from polyamine starved and unstarved bacteria showed that polyamines stimulate the synthesis of beta beta' subunits of RNA polymerase 2- to 3.6-fold.
D004279:DNA, Viral / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D005470:Fluorometry; D046911:Macromolecular Substances; D011073:Polyamines / Q000502:physiology*; D011700:Putrescine / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D013604:T-Phages / Q000235:genetics
D004279:DNA, Viral; D046911:Macromolecular Substances; D011073:Polyamines; D012321:DNA-Directed RNA Polymerases; D011700:Putrescine; D012293:Rifampin