Sensitization of Escherichia coli B/r to X-irradiation by 2'-chloro-2'-deoxythymidine.
The sensitizing effect of 2'Cl-TdR has been investigated from the viewpoint of the formation of free radicals at the C-2' position of 2'Cl-TdR when reacted with hydrated electrons. E. coli B/r cells were incubated in growth medium containing 2'Cl-TdR. Centrifugation experiments using CsCl equilibrium density gradients were carried out to confirm the incorporation of 2'Cl-TdR into DNA of the cells. The sedimentation profiles of DNA from cells grown in a medium containing 2'Cl-TdR were found at relatively heavier density positions in comparison with those of DNA from control cells. This result confirms that 2'Cl-TdR was incorporated into DNA. E. coli B/r cells which incorporated 2'Cl-TdR were more sensitive than the control cells for killing by X-irradiation under aerobic conditions. On the other hand, no difference in U.V.-inactivation curves between the cells grown in growth medium containing 2'Cl-TdR and the control cells was observed. From experiments using alkaline sucrose density gradient centrifugation, an increase in the frequency of radiation-induced single strand breaks of E. coli DNA containing 2'Cl-TdR was observed, compared with those of DNA from control cells. These results reveal that the sensitization of 2'Cl-TdR originates from an increase of damage at the sugar moiety in DNA.
D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism / Q000528:radiation effects*; D004926:Escherichia coli / Q000528:radiation effects*; D005609:Free Radicals; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013936:Thymidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014965:X-Rays
D003470:Culture Media; D004269:DNA, Bacterial; D005609:Free Radicals; D011838:Radiation-Sensitizing Agents; C038765:2'-chlorothymidine; D013936:Thymidine