Induction of unresponsiveness to skin allografts in adult mice disparate at defined regions of the H-2 complex. II. Effect of pre-graft donor-specific blood transfusions in ALS-treated mice.
The effect of pretransplant donor-specific blood transfusions on the survival of subsequent skin allografts was studied in adult congenic B10 mice disparate at various regions of the H-2 complex. Donor-host combinations were used that were disparate either at isolated K or I regions or at combined K/D and I regions. Recipient mice were given a single donor-specific blood transfusion ten days before antilymphocyte serum (ALS) treatment and skin grafting. A beneficial effect of donor-specific blood transfusions on skin graft survival was observed only in donor-recipient combinations that differed at the K and I regions. Disparity at the IA and IJ subregions appeared to be particularly important in obtaining the transfusion effect. Donor-specific transfusion had no effect on graft survival in donors and recipients disparate at only the K region, the D region plus part of the I region, or the entire H-2 complex. Pregraft transfusion in mice disparate at the I region sensitized the recipients to subsequent skin grafts from the blood donor. These results indicate that both antibody and suppressor cells play a role in the induction of the beneficial effect obtained from pretransplant donor specific transfusions.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology; D001803:Blood Transfusion; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D014181:Transplantation Immunology
D000961:Antilymphocyte Serum; D006183:H-2 Antigens