Homogeneous enzyme immunoassay of diosgenin and its glycosides.
Homogeneous enzyme immunoassay has been used as a tool for the determination of diosgenin and its glycosides in plants. Diosgenin antisera was found to inhibit the activity of diosgenin hemisuccinate-horseradish peroxidase conjugate which was reversed by the addition of free diosgenin or its glycosides. The increase of enzyme activity was proportional to the quantity of the hapten over a certain range of hapten concentration. Thus, a minimum of 2.5 micrograms/ml of diosgenin and 11.5 micrograms/ml of diosgenin glycosides could be determined by this method. The results were comparable with those obtained by high-performance liquid chromatography and gravimetric methods.
D002851:Chromatography, High Pressure Liquid; D004144:Diosgenin / Q000032:analysis*; D006027:Glycosides / Q000032:analysis; D007124:Immunoenzyme Techniques; D010944:Plants / Q000032:analysis*; D012502:Sapogenins / Q000032:analysis*
D006027:Glycosides; D012502:Sapogenins; D004144:Diosgenin