Use of homocysteic acid for selecting mutants at the gltS locus of Escherichia coli K12.
L-Homocysteic acid is toxic to Escherichia coli K12. Sensitivity to this compound is higher in cells which can utilize glutamate as sole carbon source via the Na+-dependent glutamate transport system. Such cells become resistant by mutation at the gltS locus. Sensitivity of both wild-type and glutamate-utilizing strains is greater if cells are growing on acetate as compared with glucose as major carbon source.
D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005971:Glutamates / Q000235:genetics*; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009154:Mutation
D005971:Glutamates; D006710:Homocysteine; C007956:homocysteic acid