Binding of insulin to its receptor impairs recognition by monoclonal anti-insulin antibodies.
The interaction between insulin and its receptor was investigated using both monoclonal and polyclonal anti-insulin antibodies. After covalent cross-linking of 125I-insulin to the insulin receptor on cultured human lymphocytes (IM-9 cells) using disuccinimidyl suberate, we inquired whether the insulin-receptor complex could be immunoprecipitated with anti-insulin antibodies. While a polyclonal guinea pig anti-insulin antiserum succeeded in immunoprecipitating receptor-bound 125I-insulin, binding to the receptor decreased the avidity of the antiserum for the insulin moiety by a factor of approximately 1000-fold. Sixteen distinct monoclonal murine anti-insulin antibodies were employed to immunoprecipitate receptor-bound 125I-insulin. Of these 16 monoclonal antibodies, only one (antibody 5.9F4) could be shown to recognize receptor-bound 125I-insulin. Moreover, even with antibody 5.9F4, binding of 125I-insulin to its receptor reduced the affinity of the antibody by a factor of 10- to 100-fold. These data strongly suggest that, when insulin binds to its receptor, the majority of the insulin molecule is unavailable for binding by anti-insulin antibodies. It seems likely that the hormone binding site on the receptor may be very large, thereby allowing for sequestration of the majority of the insulin molecule with relatively little of the hormone remaining exposed.
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D006168:Guinea Pigs; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007330:Insulin Antibodies / Q000378:metabolism*; D051379:Mice; D011233:Precipitin Tests; D011869:Radioligand Assay; D011972:Receptor, Insulin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D007328:Insulin; D007330:Insulin Antibodies; D011972:Receptor, Insulin