title: Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli.
abstract: The structural gene for the respiratory D-lactate dehydrogenase of Escherichia coli, a membrane-bound flavoenzyme, has been subcloned from a 7 X 10(3)-base-pair chromosomal HindIII fragment containing the gene [Young, I. G., Jaworowski, A., and Poulis, M. (1982) Biochemistry 21, 2092-2095]. The complete nucleotide sequence of the 2340-base-pair PstI-SmaI subclone has been determined on both strands by the dideoxy chain termination method. A single large open reading frame is present in the nucleotide sequence. The reading frame is preceded by a good ribosome binding site and numerous possible promoter sequences, and is followed by a typical rho-independent termination sequence. The reading frame predicts that the D-lactate dehydrogenase polypeptide consists of 571 amino acids (including the initiating methionine residue) with Mr = 64613. The protein does not have a low overall polarity, nor does it contain unusually hydrophobic stretches. It appears to contain a short repeat which is homologous with the well characterized L-lactate dehydrogenases in the vicinity of the 'essential' cysteine residue. Apart from this, homology with other proteins of known sequence has not been detected.
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D005075:Biological Evolution; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D003062:Codon; D003201:Computers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D010101:Oxygen Consumption; D010446:Peptide Fragments / Q000302:isolation & purification; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D010446:Peptide Fragments; D007770:L-Lactate Dehydrogenase