Possible causes of the loss of specific pBR322-Ad h 1 DNA recombinants following transfection.
Characteristics of pBR322/Ad h 1 DNA recombinants were studied which had been cloned using HindIII restriction endonuclease in a single "shot-gun" experiment. Both oxytetracycline and ampicillin resistance of the clones were found to be heterogeneous. Ad h 1 DNA fragments HindIII-A, and -C could be cloned only in combination with other fragments. Among the possible reasons of the loss of recombinants upon transfection the impairment of pBR322-specific gene functions by the inserts is discussed in addition to the increased tetracycline resistance, and the tertiary structure of recombinant DNA.
D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics*; D000667:Ampicillin / Q000494:pharmacology; D003001:Cloning, Molecular / Q000187:drug effects*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects; D005814:Genes, Viral / Q000187:drug effects; D006801:Humans; D009690:Nucleic Acid Conformation / Q000187:drug effects; D010118:Oxytetracycline / Q000494:pharmacology; D010403:Penicillin Resistance; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection / Q000187:drug effects*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D000667:Ampicillin; D010118:Oxytetracycline