title: Identification of the purC gene product of Escherichia coli.
abstract: The purC region of the Escherichia coli chromosome was isolated from in vivo-derived lambda transducing bacteriophages and cloned in high-copy-number plasmids. The product of the purC gene, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, was identified as a protein with an Mr of ca. 27,000. The level of the protein is increased by more than 60-fold in strains carrying the gene on a high-copy-number plasmid. Purine addition represses the enzyme level in both plasmid- and non-plasmid-containing strains.
mesh_terms: D000225:Adenine / Q000494:pharmacology; D003001:Cloning, Molecular; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D010453:Peptide Synthases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014161:Transduction, Genetic
chemical_list: D010453:Peptide Synthases; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase; D000225:Adenine