Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay.
A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two-site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D010961:Plasmodium / Q000276:immunology / Q000302:isolation & purification*; D010962:Plasmodium berghei / Q000276:immunology / Q000302:isolation & purification*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens