Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites.
A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology
D000954:Antigens, Surface; D004247:DNA