[Demonstration of circulating immune complexes using a C1q-solid phase enzyme immunoassay].
An enzyme-immunoassay for the quantitative determination of circulating immune complexes in human serum is described. The technique uses alkaline phosphatase-conjugated anti-human IgG to detect immune complexes bound to solid phase C1q. This assay is sensitive detecting 4 ng/ml to 60 micrograms/ml aggregated IgG. First data from normal individuals and patients with systemic lupus erythematosus show that this method is specific and sensitive for detection of circulating immune complexes.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D003166:Complement Activating Enzymes; D015922:Complement C1q; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D008180:Lupus Erythematosus, Systemic / Q000276:immunology
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D015922:Complement C1q; D003166:Complement Activating Enzymes
Nachweis zirkulierender Immunkomplexe mittels eines C1q-Festphasen-Enzymimmunoassays.