Optimal fixation of chick synaptosomes using different osmotic pressures.
Chick synaptosomes were found to remain intact following fixation in the presence of 0.2 M sucrose or 0.3 M sucrose. Increasing the sucrose concentration up to 0.4 M significantly decreased the number of intact synaptosomes; however, typical intrasynaptosomal organelles such as synaptic vesicles and mitochondria were visible in all preparations. This work suggests possibly important species differences in synaptosomal structure between chick and rat synaptosomes.
D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002645:Chickens; D006652:Histological Techniques; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009997:Osmotic Pressure; D013395:Sucrose; D013574:Synaptosomes / Q000648:ultrastructure*
D013395:Sucrose