Long-term culture of neurones from human cerebral cortex in serum-free medium.
A method for cultivating neurones from the fetal human central nervous system in the absence of glial cells is described. Brain cells from 15-18-week-old human fetuses are plated on polylysine-coated surfaces and grown in a serum-free hormonally-defined medium. About 98% of the cells were identified as neurones using tetanus toxin as a marker. The cultures survive for up to 7 weeks and develop an extremely complex network of neurites.
D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D003470:Culture Media; D003584:Cytological Techniques; D005333:Fetus; D006801:Humans; D009474:Neurons / Q000166:cytology*; D011107:Polylysine; D013997:Time Factors
D003470:Culture Media; D011107:Polylysine