Transformation of human diploid cells by adenovirus type 4 irradiated with ultraviolet light.
Inoculation of ultraviolet (UV)-irradiated adenovirus type 4 (Ad4) led to in vitro transformation of human diploid cells (HDC). Two transformed cell lines could be established: cell line H 1418, from HDC inoculated with the 10(-3) dilution of Ad4 UV-irradiated for 20 min at a distance of 20 cm, co-cultivated with uninfected HDC, and cell line H 1557, from HDC inoculated with the 10(-2) dilution of Ad4 irradiated at the same distance for 12 min. Both transformed cell lines were resistant to superinfection with homologous virus. Virus-specific antigen could be made evident by the indirect immunofluorescence technique in the nuclei of both H 1418 and H 1557 cells.
D000260:Adenoviruses, Human / Q000235:genetics / Q000528:radiation effects*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002472:Cell Transformation, Viral / Q000528:radiation effects*; D002478:Cells, Cultured; D006224:Cricetinae; D004171:Diploidy; D005455:Fluorescent Antibody Technique; D006801:Humans; D014466:Ultraviolet Rays
D000956:Antigens, Viral