Regional mapping of the human gene for lysosomal alpha-glucosidase by in situ hybridization.
The current approach to the chromosomal localization of genes coding for lysosomal enzymes has been the correlation of enzymatic and karyotypic analyses of human-rodent somatic cell hybrids. The feasibility of regional mapping depends on the availability of human cells with informative chromosomal rearrangements. In this communication we report the first localization of a gene coding for a lysosomal enzyme by in situ hybridization. The application of an acid alpha-glucosidase cDNA probe to normal human chromosomes allowed direct regional mapping of the alpha-glucosidase locus (GAA) to the region q23----q25 of chromosome 17.
D002874:Chromosome Mapping; D002902:Chromosomes, Human, 16-18; D003001:Cloning, Molecular; D005796:Genes; D005959:Glucosidases / Q000235:genetics*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D000520:alpha-Glucosidases / Q000235:genetics*
D005959:Glucosidases; D000520:alpha-Glucosidases