Correlation of erythrocytic peripheral blood chimaerism and putative bone marrow precursors bearing erythroid differentiation (B-G) antigens using monoclonal antibodies immobilized on fluorescent latex spheres.
The degree of erythroid chimaerism from chickens made chimaeric following stem cell infusion as embryos has been determined using a sensitive and simple procedure with monoclonal antibodies (MAbs) specific for erythroid differentiation (B-G) antigens. Highly labelled fluorescent latex beads coupled to specific MAbs have allowed for the detection of very low frequency chimaeric cell subpopulations. A direct correlation between the chimaerism of the bone marrow cells, from which mature RBCs had been eliminated, and the percentage RBC chimaerism in the blood was found. This comprises additional evidence that the B-G bearing bone marrow cells are precursors for mature RBCs.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology; D001854:Bone Marrow Cells; D002642:Chick Embryo; D002645:Chickens; D002678:Chimera; D004920:Erythropoiesis; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D007158:Immunologic Techniques; D008863:Microspheres
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens