The use of semiautomatic morphometry in the study of normal rat gastrocnemius muscle fibers.
Based on a fixed number of EM photomicrographs, a semiautomatic image analysis is made of normal gastrocnemius muscle fibers to obtain reference values for comparison with pathologic data. Z disc, mitochondria, tubular system, and sarcoplasmic reticulum are measured. It is stated that no definite fiber type differentiation is possible based on these quantitative data. However, measurements of Z disc and mitochondria are suggestive of such a differentiation. On the other hand, all data reach a Gaussian distribution on a logarithmic scale after a 5% correction factor is introduced.
D000818:Animals; D003201:Computers; D006652:Histological Techniques / Q000295:instrumentation*; D008297:Male; D008931:Mitochondria, Muscle / Q000648:ultrastructure; D009121:Muscle Denervation; D009132:Muscles / Q000648:ultrastructure*; D009210:Myofibrils / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure