Comparative characterization of Venezuelan Trypanosoma cruzi stocks by electron microscopy, isoelectrofocusing and lectin typing.
The intraspecific variation among culture forms of 14 Venezuelan Trypanosoma cruzi stocks were examined by kDNA configuration, isoenzymes, and agglutination behaviour of lectins. The results have shown that in all of the stocks the central band of kDNA is present, showing that the stocks are parasites of the subgenus Schizotrypanum. By isoenzymes and lectin typing it has been found that the stocks belong to the isoenzyme group I and the lectin-type PNA which were already described for other Venezuelan stocks. The homogeneous results of intraspecific characterization contrast to those found in other countries in South America south of the Amazon basin and seem to be a further evidence that in countries north of the Amazon basin mainly on T. cruzi-type exists.
D000372:Agglutination Tests; D000818:Animals; D004247:DNA; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000145:classification*; D037102:Lectins / Q000494:pharmacology*; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D014687:Venezuela
D007527:Isoenzymes; D037102:Lectins; D004247:DNA