Use of monoclonal antibodies in enzyme linked immunosorbent assay (ELISA) for detection of botulinum type B toxins.
Use of polyclonal antibodies failed to correlate mouse assay with enzyme linked immunosorbent assay (ELISA) in titration of culture fluid of different strains of Clostridium botulinum type B. If ELISA is performed with such a monoclonal antibody that is capable of neutralizing the toxin, however, the lethal toxicity can be determined quantitatively.
D000818:Animals; D000911:Antibodies, Monoclonal; D001905:Botulinum Toxins / Q000032:analysis* / Q000633:toxicity; D003014:Clostridium botulinum / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007928:Lethal Dose 50; D051379:Mice
D000911:Antibodies, Monoclonal; D001905:Botulinum Toxins