Intravenous injection of mice with bacteroides fragilis and Escherichia coli: role of Thioglycollate medium in the infectious process.
Mice were intravenously infected with E. coli, B. fragilis, and combinations of both, and the bacterial contents of livers, kidneys, and spleens were monitored from the first up to the 9th day postinfection. If Thioglycollate medium was added to otherwise sublethal doses of E. coli, the inoculum was able to induce severe and for some animals lethal infections. B. fragilis was not capable to produce prolonged infections; however, when given together with E. coli and Thioglycollate medium, both bacteria were recovered from some animal organs even on day 9 postinfection. Employing this experimental model of a mixed anaerobic/facultative bacterial infection, previous reports attributing animal lethality to E. coli were confirmed, but a synergism between E. coli and B. fragilis could not be demonstrated.
D000818:Animals; D001442:Bacteroides Infections / Q000150:complications / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000302:isolation & purification / Q000472:pathogenicity*; D003470:Culture Media; D004926:Escherichia coli / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology*; D005260:Female; D007275:Injections, Intravenous; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D051379:Mice; D013154:Spleen / Q000382:microbiology; D013864:Thioglycolates / Q000494:pharmacology
D003470:Culture Media; D013864:Thioglycolates