Rapid enzyme-linked immunosorbent assay (ELISA) for the detection of IgG antibodies against Micropolyspora faeni and Thermoactinomyces vulgaris.
A rapid ELISA was developed for the detection of specific IgG against Micropolyspora faeni and Thermoactinomyces vulgaris and compared with counterimmunoelectrophoresis (CIE) in twenty-seven patients with suspected farmer's lung disease (FLD). Seventeen patients had precipitins to M. faeni or T. vulgaris or both, and ten had no precipitins. The optimum conditions for each step in the ELISA were determined: pre-equilibration of reagents at 37 C and vigorous agitation during incubation enabled the total test time required for the procedure to be reduced to 4 hr. A serum dilution of 10-fold produced good differentiation between CIE-positive and -negative sera. Little correlation was seen between CIE and ELISA for either M. faeni or T. vulgaris antigens in tests with sera from patients with precipitins: high readings were often recorded in ELISA where no precipitins had been detected with the same antigen. Positive-negative discrimination of unknown sera in ELISA was achieved through the inclusion of CIE-positive and -negative reference sera in each test run. Thirteen CIE-positive sera were classed as positive in ELISA with the M. faeni antigen while eight of thirteen CIE-positive sera were positive in ELISA with the T. vulgaris antigen. For both antigens, four CIE-negative sera were recorded as positive in ELISA.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000907:Antibodies, Bacterial / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005203:Farmer's Lung / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D008848:Micromonosporaceae / Q000276:immunology*; D011234:Precipitins / Q000032:analysis; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D011234:Precipitins