title: Effect of insulin on free fatty acid uptake by hepatocytes in the duck.
abstract: To elucidate the hypolipacidaemic effect of insulin in ducks, its action on the uptake of free fatty acids (FFA) by duck hepatocytes was determined. At low doses (10 mu./l) insulin stimulated FFA uptake. This effect was not observed with higher doses of insulin (20, 30 and 50 mu./l). Growth hormone at physiological concentrations and corticosterone (14.4 nmol/l) decreased basal activity, probably by reducing glucose metabolism and consequently alpha-glycerophosphate (alpha-GP) supply. Insulin was able to reverse the inhibition induced by GH and corticosterone on both FFA uptake and alpha-GP production. These results therefore suggest that the hypolipacidaemic effect of insulin may be partly mediated by its action on hepatic FFA uptake.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology; D004372:Ducks; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005994:Glycerophosphates / Q000096:biosynthesis; D013006:Growth Hormone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D005230:Fatty Acids, Nonesterified; D005994:Glycerophosphates; D007328:Insulin; D013006:Growth Hormone; C029620:alpha-glycerophosphoric acid; D003345:Corticosterone