Insulin modulates thyrotropin-induced follicle reconstruction and iodine metabolism in hog thyroid cells cultured in a chemically defined medium.
Effects of insulin on cultured hog thyroid cells were examined in a chemically defined medium supplemented with thyrotropin. Thyrotropin-induced follicle reconstruction and recovery of functional activities were stimulated by the addition of insulin to the culture medium. The iodide uptake and organification at the maximum stimulation exceeded 3 times those of the control values. The minimum effective dose of insulin was 60 microU/ml. The insulin stimulation occurred after a latency period of 24 h. Since no growth promoting effect of insulin was observed during the cultivation period in the present study, insulin appears to act in concert with thyrotropin in regenerating the characteristic properties of the differentiated cells during cultivation.
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D007328:Insulin / Q000494:pharmacology*; D007454:Iodides / Q000378:metabolism*; D007700:Kinetics; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013972:Thyrotropin / Q000494:pharmacology*
D003470:Culture Media; D007328:Insulin; D007454:Iodides; D013972:Thyrotropin