Liquid nitrogen storage of yeast cultures. II. Stability of characteristics of stored strains.
Nineteen strains of yeasts possessing different characteristics were stored in liquid nitrogen and after 5 years phenotypic characters were evaluated and compared with equivalent strains preserved under paraffin oil. All qualitative characters tested remained stable, and quantitative characters varied only within the range of natural variability.
D005615:Freezing; D008828:Microbiological Techniques; D009584:Nitrogen; D009821:Oils; D010232:Paraffin; D011309:Preservation, Biological; D015003:Yeasts / Q000502:physiology*
D009821:Oils; D010232:Paraffin; C015418:paraffin oils; D009584:Nitrogen