[Study on the new development of highly sensitive E2-EIA used by two specific binding systems and on the clinical application].
As a tracer, biotin was bound to the amino group of newly synthesized 6-ethylenediamino-17-beta-estradiol. Two competitive methods were developed by using the tracer (E2-B). Method I, E2-B, sample, and anti E2 antibody conjugated with horse radish peroxidase were mixed, and the mixture was added to the avidin immobilized on a microplate to separate free and bound tracer. Then the enzyme activity which remained after washing was measured by using H2O2 and o-phenylenediamine. E2 was quantified from 75 pg/ml to 9.6 ng/ml; (II) E2-B and the samples were added to the anti E2 adsorbed on a microplate. After washing, the avidin conjugated with HRP was put into the immune complex plate, and the enzyme activity which remained was determined to calculate E2. The standard curve indicated E2 from 75 pg/ml to 19.2 ng/ml. The E2 values obtained by the new methods correlated with those by 3H-RIA and the EIA was useful in monitoring the induction of ovulation.
D000328:Adult; D000858:Anovulation / Q000097:blood / Q000188:drug therapy; D001360:Avidin; D001667:Binding, Competitive; D001710:Biotin; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008991:Monitoring, Physiologic / Q000379:methods; D010062:Ovulation Induction; D011247:Pregnancy; D011485:Protein Binding
D006063:Chorionic Gonadotropin; D001360:Avidin; D004958:Estradiol; D001710:Biotin