Enrichment of natural killer cells by negative selection: comparison to Percoll gradient separation method.
A method is described for the preparation of human peripheral blood mononuclear cell (PBMC) suspensions containing highly enriched natural killer (NK) cell cytotoxicity. The technique involved the negative selection of OKM1+ cells by the selective removal of nylon wool nonadherent PBMC which are reactive with the Leu-1 monoclonal antibody. The Leu-1+ cells are removed by subsequent rosette formation with anti-mouse IgG coated bovine erythrocytes. The resultant OKM1+ cell suspension had a greater number of large granular lymphocytes, K562 target binding effector cells, and lytic activity than concomitantly prepared fractions of Percoll gradients.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology*; D010641:Phenotype
D000911:Antibodies, Monoclonal