Antigen beads: a novel approach for the delivery of a soluble Anaplasma immunogen.
An important consideration in the development of effective immunization regimens against bovine anaplasmosis is the optimization of vaccine delivery systems. This report describes the formulation of proteinaceous antigen-containing spheres derived from supernatant fluids of Analplasma marginale cultures. The antigen beads were designed for use as potential dispersal agents for a soluble Anaplasma immunogen. Immunospecificity assays demonstrated that A marginale antigen was located on the surfaces of individual antigen beads. Use of Anaplasma antigen beads with a potent adjuvant, such as saponin, was proposed.
D000276:Adjuvants, Immunologic; D000709:Anaplasma / Q000276:immunology*; D000712:Anaplasmosis / Q000517:prevention & control; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007120:Immunochemistry; D012503:Saponins; D012995:Solubility
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007106:Immune Sera; D012503:Saponins