Monoclonal antibodies to bovine somatotropin: immunoadsorbent reagents for mammalian somatotropins.
Twenty-nine stable hybridoma cell lines secreting monoclonal antibodies to bovine somatotropin (bST) have been produced and characterized. Five of the monoclonal antibodies bind porcine and human somatotropins as well as bST. One of these antibodies was used as a reagent in immunoadsorbent chromatography of recombinant bST or pituitary bST from cell extracts. Following chromatography, the bST preparations retained activity in a rabbit liver radioreceptor assay and in a radioimmunoassay. The immunoadsorbent reagent bound human and porcine somatotropins as well as bovine.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology*; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D013006:Growth Hormone / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D013006:Growth Hormone