Chemiluminescent immunosorbent assay of serum myoglobin based on the luminol reaction.
A simple and sensitive method for the determination of serum myoglobin is described. Myoglobin was determined in the range of 10-500 micrograms/1 by the chemiluminescent luminol reaction after adsorption to anti-myoglobin IgG onto a solid phase. Only 50 microliter of serum has to be used and the luminescent immunoassay (LIA) has a better sensitivity and a wider linear range than the conventional radioimmunoassay (RIA). However, in its present form LIA has a higher imprecision than RIA. In clinical specimens the correlation between the two methods was excellent.
D000918:Antibody Specificity; D003402:Creatine Kinase / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D007527:Isoenzymes; D008163:Luminescent Measurements; D008165:Luminol; D008832:Microchemistry; D009203:Myocardial Infarction / Q000097:blood; D009211:Myoglobin / Q000097:blood* / Q000276:immunology; D011724:Pyridazines; D011863:Radioimmunoassay
D007074:Immunoglobulin G; D007527:Isoenzymes; D009211:Myoglobin; D011724:Pyridazines; D008165:Luminol; D003402:Creatine Kinase