Genital tract proteins in the male rabbit: II. Alkaline phosphatase--enzyme action and site of synthesis.
An alkaline phosphatase was isolated from rabbit seminal plasma by ion-exchange chromatography, gel-filtration and preparative isoelectric focusing. Enzyme inhibition with Na2-EDTA was mostly effectively reverted by means of Mg2+, Zn2+ and Ca2+ in the incubation medium. The use of a monospecific antiserum against the seminal plasma alkaline phosphates permitted to identify through immunofluoresceine the ductus epididymis, deferens and its ampullae as the main site of synthesis of this protein.
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000302:isolation & purification*; D000818:Animals; D004822:Epididymis / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005837:Genitalia, Male / Q000201:enzymology*; D008297:Male; D011817:Rabbits; D012661:Semen / Q000201:enzymology*; D014649:Vas Deferens / Q000201:enzymology
D000469:Alkaline Phosphatase