The early effect of sublethal X-irradiation of phagocytic cells in mouse blood and the influence of cystamine as measured by chemiluminescence.
The metabolic burst accompanying phagocytosis of granulocytes (PMN) leads to the generation of activated oxygen species such as O-2, H2O2, 1O2 and OH; which give rise to chemiluminescence (CL) in the presence of luminol. Reliable CL-measurements of stimulated PMN can be carried out in freshly drawn mouse blood, when photon counts are related to the number of PMN. Effects of low dose total body X-irradiation were studied using C57B1/6 mice. It was found that 24 and 48 hours after irradiation (0.24-0.95 Gy) CL of whole blood was slightly decreased. If however CL-counts were related to the number of PMN, an enhanced CL per single granulocyte was recorded. The administration of cystamine leads to an immune stimulating effect of unirradiated animals. In animals, who received 0.95 Gy a distinct radioprotective effect of cystamine can be observed.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D003538:Cystamine / Q000008:administration & dosage*; D007958:Leukocyte Count; D008163:Luminescent Measurements; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D013997:Time Factors; D014916:Whole-Body Irradiation; D015054:Zymosan / Q000494:pharmacology
D011837:Radiation-Protective Agents; D000244:Adenosine Diphosphate; D015054:Zymosan; D003538:Cystamine