In vivo analysis of impaired macrophage bactericidal capacity during experimental African trypanosomiasis.
Since innate resistance of mice to Salmonella typhimurium depends on an intact macrophage system, we have used this bacterium to investigate the effect of Trypanosoma brucei subsp. rhodesiense infection on macrophage phagocytic and cytolytic function. CBA/CaJ mice infected with T. brucei subsp. rhodesiense have decreased resistance to S. typhimurium, since doubly infected mice rapidly succumb to sublethal doses of S. typhimurium. Although trypanosomiasis is known to suppress antibody formation, such a suppression of antibody does not seem to play a role in trypanosome-induced sensitivity to S. typhimurium. A trypanosome-induced blockade of the reticuloendothelial system also does not occur, since parasitized and control mice clear S. typhimurium from the blood equally well. Early killing (0 to 48 h) of S. typhimurium in the liver and spleen is mainly macrophage mediated, and mice infected with trypanosomes kill S. typhimurium in the liver and spleen very poorly. Apparently trypanosomiasis inhibits macrophage bactericidal activity, but has no effect on phagocytosis.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001770:Blood Bactericidal Activity; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010587:Phagocytosis; D012480:Salmonella Infections / Q000276:immunology*; D012486:Salmonella typhimurium; D014353:Trypanosomiasis, African / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology
D000907:Antibodies, Bacterial