Effect of 60Co-irradiation on the development and immunogenicity of Plasmodium berghei sporozoites in Anopheles stephensi mosquitoes.
Protection conferred to mice by Plasmodium berghei sporozoites increased significantly when the time interval between 60Co-irradiation of the infected mosquitoes and harvest of sporozoites increased. One thousand sporozoites conferred no protection against challenge if harvested on the day of irradiation, but protected 60% of recipient mice when harvested 28 days postirradiation. When the time between feeding of mosquitoes and irradiation was varied, sporozoites from mosquitoes irradiated 3 days after feeding were infective for mice. Sporozoites from mosquitoes irradiated on day 10 postfeeding were not infective, but were immunogenic. In all experiments a decline occurred in the number of recoverable sporozoites over a 28-day period postirradiation to less than 10% of the yield on the day of irradiation.
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000917:Antibody Formation; D003037:Cobalt Radioisotopes; D007114:Immunization; D008288:Malaria / Q000517:prevention & control*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology / Q000502:physiology / Q000528:radiation effects*; D013997:Time Factors
D003037:Cobalt Radioisotopes