[Determination of primary amines and protein by using fluorescamine in microorganism suspensions].
The paper describes a fluorimetric technique for determining the overall amount of primary amines and protein using fluorescamine directly in suspensions of yeast and bacterial microorganisms. The optimal conditions are selected for the reaction. The technique is highly sensitive and has an advantage over biochemical methods when one has to make many assays of amine containing compounds in biological material. The technique can find wide application in industrial practice.
D000588:Amines / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D002175:Candida; D005450:Fluorescamine / Q000032:analysis*; D005656:Fungal Proteins / Q000032:analysis*; D006090:Gram-Negative Bacteria; D006094:Gram-Positive Bacteria; D007202:Indicators and Reagents / Q000032:analysis; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence / Q000379:methods; D013141:Spiro Compounds / Q000032:analysis*; D013535:Suspensions
D000588:Amines; D001426:Bacterial Proteins; D005656:Fungal Proteins; D007202:Indicators and Reagents; D013141:Spiro Compounds; D013535:Suspensions; D005450:Fluorescamine
Opredelenie pervichnykh aminov i belka c pomoshch'iu fluoreskamina v suspenziiakh mikroorganizmov.