title: Expression of a retrovirus encoding human HPRT in mice.
abstract: Transmissible retroviruses encoding human hypoxanthine phosphoribosyltransferase (HPRT) were used to infect mouse bone marrow cells in vitro, and the infected cells were transplanted into mice. Both active human HPRT-protein and chronic HPRT-virus production were detected in hematopoietic tissue of the mice, showing transfer of the gene. These results indicate the possible use of retroviruses for somatic cell therapy.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000382:microbiology; D016026:Bone Marrow Transplantation; D004274:DNA, Recombinant / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000382:microbiology; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007527:Isoenzymes / Q000378:metabolism; D007926:Lesch-Nyhan Syndrome / Q000235:genetics / Q000628:therapy; D051379:Mice; D009693:Nucleic Acid Hybridization; D051381:Rats; D012190:Retroviridae / Q000201:enzymology / Q000235:genetics*; D013154:Spleen / Q000382:microbiology
chemical_list: D004274:DNA, Recombinant; D007527:Isoenzymes; D007041:Hypoxanthine Phosphoribosyltransferase