Distribution of the major histocompatibility complex antigens on different cellular components of human liver.
Monoclonal mouse antibodies to the "framework" determinants of the class I and II molecules of the major histocompatibility complex (MHC) were used to demonstrate the presence of the MHC antigens in human liver. First, the localization of these antigens was demonstrated from frozen section histology with indirect FITC immunofluorescence and the cell component(s) binding the mouse antibody were identified by rabbit marker antisera and indirect TRITC immunofluorescence. Second, the antigen expression on the cell surface was analyzed by the Staphylococcus aureus rosette method from cytological cell smears. All antibodies reacted with cells in the liver sinusoids, both with the Kupffer cells and at least partially with the sinusoidal endothelial cells. The same antisera reacted also with the bile duct cells, though weaker, and with some stromal cells in close proximity of the blood vessels. The vascular endothelial cells of hepatic artery, hepatic vein, and portal vein displayed no reaction. Thus human liver differs strikingly from, e.g., human kidney, where the vascular endothelial cells contain large amounts of MHC antigens on the cell surface. This difference may be one explanation to why liver allografts are less promptly rejected than renal allografts in man.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001808:Blood Vessels / Q000276:immunology; D002449:Cell Aggregation; D004727:Endothelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007728:Kupffer Cells / Q000276:immunology; D008099:Liver / Q000098:blood supply / Q000276:immunology*; D008285:Major Histocompatibility Complex
D000911:Antibodies, Monoclonal