The dissociation of evoked release of [3H]-GABA and of endogenous GABA from chick retina in vitro.
The release of prelabeled [3H]-GABA and endogenous GABA evoked by KCl and excitatory amino acid analogs was compared in intact chick retina and retina previously lesioned by the neurotoxins, kainic acid and N-methyl-D,L-aspartic acid. Significant discrepancies were found in the results obtained by the two techniques; and while changes in endogenous GABA release after neurotoxin lesions correlated with retinal glutamate decarboxylase activity, a marker for GABAergic neurons, the release of [3H]-GABA did not. These results call into question the validity of the prelabeling technique for studying GABA release from retina.
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002645:Chickens; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology; D016202:N-Methylaspartate; D010477:Perfusion; D011189:Potassium Chloride / Q000494:pharmacology; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
D014316:Tritium; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D011189:Potassium Chloride; D007608:Kainic Acid