Determination of tissue plasminogen activator by an enzyme-immunoassay method.
The sensitive assay method of tissue plasminogen activator was established by an enzyme-immunoassay method, and discriminates tissue (nonurokinase) type plasminogen activator from urokinase. The sensitivity was 0.1 ng/assay tube, and the plasma concentration of tissue plasminogen activator in normal healthy subjects was 1.22 +/- 0.25 ng/ml. Distribution of tissue plasminogen activator was examined in normal tissue. A melanoma cell line was employed as cell culture medium for determination of tissue plasminogen activator.
D000368:Aged; D002460:Cell Line; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008297:Male; D008545:Melanoma / Q000032:analysis; D008875:Middle Aged; D010960:Plasminogen Activators / Q000302:isolation & purification*
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010960:Plasminogen Activators