The detection and analysis of chitinase activity from the yeast form of Candida albicans.
Chitinase activity was detected in the supernatant fraction of a high-speed centrifugation preparation of broken Candida albicans yeast cells. The enzyme showed peak activity during the rapid budding phase of growth and was found to parallel the chitin synthase activity. The optimum conditions for the hydrolysis of chitin, regenerated from acetylation of chitosan, were determined. Analysis of the kinetics of the enzyme-substrate interaction and a measurement of their binding suggests that an equilibrium binding situation exists and that the kinetics follow a Langmuir isotherm interaction.
D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism*; D007700:Kinetics
D002686:Chitin; D002688:Chitinases