[Analysis of human chromosomes by the flow microcytometer].
Human peripheral lymphocytes metaphase chromosomes, prepared for flow analysis were used to evaluate the resolving power of a new microscope-based flow cytometer "Leitz MPV flow". We were able to resolve the human karyotype into about 15 peaks after simple ethidium bromide staining and excitation with mercury arc lamp. These results showed that a simple flow cytometer with a common relatively inexpensive source of fluorescence excitation light, can compete with more sophisticated laser-illuminated flow cytometers for flow cytogenetic studies.
D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure
Analyse de chromosomes humains par microcytophotomètre de flux.