Effect of S-100 protein on assembly of brain microtubule proteins in vitro.
S-100 protein inhibits the assembly of brain microtubule proteins in vitro in the presence of 10 microM free Ca2+. The S-100 effect is generally greater on the rate than on the extent of assembly, and even greater as the microtubule protein concentration decreases and the time of preincubation between S-100 and microtubule proteins before GTP addition increases, at a given S-100/tubulin dimer molar ratio. The S-100 effect is greatly enhanced in the presence of physiological concentrations of K+ and is completely reversed by EGTA.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004533:Egtazic Acid / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
D009418:S100 Proteins; D014404:Tubulin; D004533:Egtazic Acid; D006160:Guanosine Triphosphate; D011188:Potassium; D002118:Calcium