A rapid, high-yield method of producing mono-[125I]A14 iodoinsulin.
We have developed a rapid method for producing homogeneous mono-[125I]A14 iodoinsulin with high specific activity and yield. After iodination by the lactoperoxidase method, the labeled peptides were applied to a C18 Porasil pre-column, washed with aqueous buffer to eliminate the free [125I]-iodide and placed "in-line" with a C-18 HPLC column; mono-[125I]A14 iodoinsulin was then eluted isocratically with 29% acetonitrile in 16 minutes. The labeled hormone was extremely stable, and proved suitable for various biological studies.
D000818:Animals; D001717:Birds; D002700:Chloramines; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D014105:Tosyl Compounds
D002700:Chloramines; D007328:Insulin; D014105:Tosyl Compounds; C016300:chloramine-T; C008147:insulin, iodo-