Hydrolyses of alpha-naphthyl acetate, beta-naphthyl acetate, and acetyl-DL-phenylalanine beta-naphthyl ester.
Using simultaneous coupling azo dye techniques kidney enzymes active against alpha-naphthyl acetate, beta-naphthyl acetate, and acetyl-DL-phenylalanine beta-naphthyl ester are characterized. The enzymes show identical distribution in the section. The banding patterns in zymograms are the same after incubation with the different substrates. The enzymes might, however, be separated by difference in pH optimum, initial velocity and sensitivity to inhibitors and activators.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D004950:Esterases / Q000032:analysis*; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007668:Kidney / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009284:Naphthols / Q000378:metabolism*; D013053:Spectrophotometry
D009284:Naphthols; C005366:naphthyl acetate; C027876:alpha-naphthyl acetate; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; C018733:acetylphenylalanine beta-naphthyl esterase