Isoelectric focusing and isoelectric points of aminoacyl-tRNA synthetases.
Isoelectric points and isoelectric focusing behaviour of 10 highly purified eukaryotic aminoacyl-tRNA synthetases from 3 sources, Saccharomyces cerevisiae, Euglena gracilis and Phaseolus vulgaris were examined. The pI-values measured on polyacrylamide gels under native conditions are situated between pH 5.0-7.5. A microheterogeneity was observed for 9 enzymes appearing otherwise homogeneous on gel electrophoresis. A compilation of the isoelectric points of aminoacyl-tRNA synthetases is given and literature data are compared with our experimental results.
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D001419:Bacteria / Q000201:enzymology; D005056:Euglena gracilis / Q000201:enzymology; D007887:Fabaceae / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D010946:Plants, Medicinal; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013045:Species Specificity
D000604:Amino Acyl-tRNA Synthetases