Independent induction of sister-chromatid exchanges by 3-aminobenzamide and ultraviolet radiation in HeLa cells.
The effect of 3-aminobenzamide in HeLa cells was examined in several aspects. 10(-3) M 3-aminobenzamide inhibited poly(ADP-ribose) polymerase activity to more than 95% in disrupted nuclei of HeLa cells. The mode of inhibition of the enzyme was competitive inhibition with NAD at a Ki value of 2.5 microM 3-aminobenzamide. The combined treatment of HeLa cells with 3-aminobenzamide and ultraviolet radiation revealed the independent induction of SCEs by these 2 agents.
D001549:Benzamides / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003434:Crossing Over, Genetic / Q000187:drug effects*; D006367:HeLa Cells / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D006801:Humans; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide