Isolation and characterization of mitomycin-C-sensitive mouse lymphoma cell mutants.
26 mutants with increased sensitivity to the lethal effects of mitomycin C (MMC) were isolated from mouse lymphoma L5178Y cells by a replica-plating technique. Most of them were about 5-10 times more sensitive in terms of D37 values to MMC than were parental cells. 5 of the MMC-sensitive mutants isolated from independently mutagenized cell populations were further analyzed. They were highly sensitive to the killing by decarbamoyl (DC) MMC, a monofunctional derivative of MMC, but were not sensitive to ultraviolet radiation, X-rays, 4-nitroquinoline-1-oxide or methyl methanesulfonate. These 5 mutants were classified into at least 2 genetic complementation groups. The implication of these mutations in cross-link and mono-adduct repair of DNA damage induced by MMC and DCMMC is discussed.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D003432:Cross-Linking Reagents; D004260:DNA Repair; D005816:Genetic Complementation Test; D008223:Lymphoma / Q000235:genetics; D051379:Mice; D008937:Mitomycins / Q000494:pharmacology*; D009154:Mutation; D009374:Neoplasms, Experimental / Q000235:genetics; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D008937:Mitomycins; D015112:4-Nitroquinoline-1-oxide