Effects of chemical mediators of anaphylaxis on ciliary function.
We assessed the effects of selected chemical mediators of anaphylaxis on CBF in vitro. Ciliated epithelial cells were obtained from the trachea of conscious sheep with a cytology brush and suspended in a perfusion chamber containing KH. Ciliary activity was viewed microscopically and recorded on videotape for subsequent slow-motion analysis of CBF. Prostaglandin E1 (10(-8) M to 10(-6) M), prostaglandin E2 (10(-10) M to 10(-6) M), and leukotriene-C4 (10(-8) M) increased CBF between 7% and 33%. Histamine caused ciliostimulation only at the relatively high concentrations above 10(-5) M (7% increase in CBF), whereas prostaglandin F2 alpha (10(-10) M and 10(-6) M) was without effect. In no preparation was ciliary discoordination observed. These findings indicate that several chemical mediators of anaphylaxis stimulate CBF and that the previously described impairment of mucociliary transport in stable allergic asthma or antigen-induced bronchoconstriction is probably not caused by a primary alteration of ciliary function.
D000527:Alprostadil; D000818:Animals; D002245:Carbon Dioxide; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D015237:Dinoprost; D005260:Female; D006632:Histamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010100:Oxygen; D010313:Partial Pressure; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D013189:SRS-A / Q000494:pharmacology*; D012756:Sheep; D013268:Stimulation, Chemical
D011458:Prostaglandins E; D011460:Prostaglandins F; D013189:SRS-A; D002245:Carbon Dioxide; D006632:Histamine; D015237:Dinoprost; D000527:Alprostadil; D010100:Oxygen