The amount and nature of glutathione transferases in rat liver microsomes determined by immunochemical methods.
The amount and nature of glutathione transferases in rat liver microsomes were determined using immunological techniques. It was shown that cytosolic glutathione transferase subunits A plus C, and B plus L were present at levels of 2.4 +/- 0.6 and 1.5 +/- 0.1 microgram/mg microsomal protein, respectively. These levels are 10-times higher than those for non-specific binding of cytosolic components judging from the distribution of lactate dehydrogenase, a cytosolic marker. The possibility that a portion of these glutathione transferases is functionally localized on the endoplasmic reticulum is discussed. A previously described microsomal glutathione transferase which is distinct from the cytosolic enzymes is present in an amount of 31 +/- 6 micrograms/mg microsomal protein.
D000818:Animals; D000936:Antigen-Antibody Complex; D003600:Cytosol / Q000201:enzymology; D005982:Glutathione Transferase / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D005982:Glutathione Transferase