Rapid turbidimetric determination of serum C3c and C4 by end point centrifugal analysis.
An end point turbidimetric method for the determination of C3c and C4 in serum using a centrifugal analyzer (Cobas Bio) is described. Several analytical factors were evaluated -pH, temperature, PEG and antibody concentration. Wide variations of temperature and pH did not significantly affect the turbidimetric reaction. A 20 g/L PEG concentration and 25-fold antiserum dilution were found satisfactory for the analysis. Precision of the assay was good and comparison with a RID method yielded an r value of 0.97. The procedure is simple and reliable.
D002498:Centrifugation / Q000295:instrumentation; D003176:Complement C3 / Q000097:blood*; D015932:Complement C3c; D003181:Complement C4 / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D005779:Immunodiffusion; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011092:Polyethylene Glycols; D013696:Temperature; D013997:Time Factors
D003176:Complement C3; D003181:Complement C4; D007106:Immune Sera; D011092:Polyethylene Glycols; D015932:Complement C3c