A selective and differential agar for anaerobic comma-shaped bacteria recovered from patients having motile rods and non-specific vaginosis.
A selective and differential agar for optimal growth of the large curved motile anaerobic rods isolated from patients having non-specific vaginosis (NSV) was developed. A basal medium of Columbia CNA agar was used with colony growth found to be optimal by the addition of 7% fetal calf serum and 5% rabbit blood (CNARS). Beta-Haemolysis was found to be demonstrated most readily on a bilayer plate with a basal layer consisting of CNA agar with fetal calf serum, overlaid with a layer of CNA agar containing fetal calf serum and rabbit blood. Optimal growth was found by anaerobic incubation at 37 degrees C, after 5 days.
D000362:Agar; D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D014627:Vaginitis / Q000382:microbiology*
D003470:Culture Media; D000362:Agar