[Enzymatic mechanisms of degradation of DNA replication forks in vitro].
The present work provides experimental evidence of two ways of fermentative degradation of DNA replication forks in vivo. The first way proceeds under the influence of exonuclease V, which degradates DNA replication forks, uncovered by singlestrand binding (ssb) proteins. DNA replication forks protected against nucleases by ssb protein are degrated by a second way: by tandem action of DNA polymerase II with ssb protein complexes and exonuclease V. The default of normal ssb protein in cells of Escherichia coli is responsible for the first way of DNA replication forks degradation. The second way is occurs at excess of normal ssb protein.
D001426:Bacterial Proteins / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication / Q000528:radiation effects; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005720:Gamma Rays; D009154:Mutation; D010957:Plasmids; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D004257:DNA Polymerase II; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V
Fermentativnye mekhanizmy degradatsii replikativnykh vilok DNK v usloviiakh in vitro.