Chromatographic and fluorescence spectroscopic studies of individual 7,12-dimethylbenz[a]anthracene--deoxyribonucleoside adducts.
Compared with standard Sephadex LH-20 column chromatography, a newly developed high pressure liquid chromatographic separation of hydrocarbon deoxyribonucleoside adducts derived from the DNA of mouse embryo cell cultures exposed to 7,12-dimethylbenz[a]anthracene (DMBA) provides markedly superior resolution. Once resolved, the fluorescence spectroscopic properties of the three major DMBA--DNA adducts indicate that the fluorescence exhibited by adducts derived from a bay region syn dihydrodiol epoxide of DMBA differs subtly from that exhibited by adducts derived from the isomeric anti dihydrodiol epoxide.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA / Q000378:metabolism*; D004622:Embryo, Mammalian; D051379:Mice; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014316:Tritium
D001551:Benz(a)Anthracenes; D014316:Tritium; D015127:9,10-Dimethyl-1,2-benzanthracene; D004247:DNA