Crossed immunoelectrophoresis for the detection of split products of the third complement component in dengue hemorrhagic fever. II. In vitro activation by dengue viral antigen.
The ability of dengue viral antigen(s) from different sources, and performed antigen-antibody complexes, to activate the third component of human complement (C3) in vitro was demonstrated by using split products of human C3. Dengue type 2 viral antigen(s) from suckling mouse brain and from infected monocytes activated C3 via the alternative pathway, while performed soluble dengue-2 antibody complex activated mainly the classical pathway. The implications of this finding for the pathogenesis of dengue hemorrhagic fever are discussed.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D000956:Antigens, Viral / Q000378:metabolism*; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism*; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D003715:Dengue / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007962:Leukocytes / Q000382:microbiology; D008253:Macaca mulatta; D011817:Rabbits / Q000276:immunology
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D003176:Complement C3