Localization of Met5-enkephalin-Arg6-Phe7-like immunoreactivity in the rat gastrointestinal tract.
The distribution of Met5-enkephalin-Arg6-Phe7 (Met-Enk-Arg-Phe)-like immunoreactivity in the rat gastrointestinal tract was studied by immunohistochemical techniques. Antiserum against a Met-Enk-Arg-Phe-thyroglobulin conjugate was raised in rabbits and was found to be specific for synthetic Met-Enk-Arg-Phe. Met-Enk-Arg-Phe-like immunoreactivity was found in neuronal structures in all parts of the rat gastrointestinal tract. Immunostained somata were primarily located in myenteric plexus; immunostained processes were mostly present in myenteric plexus and circular muscle layer. This distribution pattern is similar to that of the three other opioid polypeptides, Met5-enkephalin, Leu5-enkephalin and Met5-enkephalin-Arg6-Gly7-Leu8.
D000818:Animals; D004064:Digestive System / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D009197:Myenteric Plexus / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013368:Submucous Plexus / Q000378:metabolism
D004744:Enkephalin, Methionine; C027511:enkephalin-Met, Arg(6)-Phe(7)-