Characterization of human interferon-gamma-producing leukocytes with monoclonal antileukocyte antibodies.
Monoclonal antibodies with specificities for subsets of human leukocytes have been used for the characterization of interferon (IFN) gamma-producing cells. The production of IFN gamma was demonstrated to be a function of OKT3+T lymphocytes. The capacity to secrete IFN gamma was not restricted to the OKT4+ or the OKT8+T-cell subset. BA-1+B lymphocytes and Leu7+ natural killer cells did not contribute to the production of IFN gamma. Ia+, OKM1+ monocytes served an auxiliary function in the production of IFN gamma. The requirement for accessory monocytes, however, was not absolute, because monocyte-free preparations of long-term cultured IL2-dependent T lymphocytes retained the capacity to secrete IFN gamma.
D000911:Antibodies, Monoclonal; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007371:Interferon-gamma