Conformational study of low molecular weight RNAs in solution by fluorescent probe.
Pseudouridine psi 73 in yeast 5.8S RNA was modified with 4-bromomethyl-7-methoxycoumarin(BMC). Temperature dependence of fluorescence intensity was measured and it was compared with UV-thermal melting curve. The region around psi 73 melts partially. Fluorescence intensity changes by titration with Mg2+ and it quenches largely by added iodide ion. The results are compared with those of BMC-modified psi 55 of tRNAPhe and psi 50 of 5S RNA reported in previous paper1-4.
D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D011560:Pseudouridine; D012331:RNA, Fungal; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014468:Umbelliferones
D012331:RNA, Fungal; D014468:Umbelliferones; D011560:Pseudouridine; C000317:4-bromomethyl-7-methoxycoumarin