Measurement of skin and total blood flow to the rabbit lower limb by washout of freely diffusible radioactive tracers introduced intra-arterially.
Good agreement was obtained when measuring total (capillary) blood flow in 9 rabbit thighs using two different techniques. The 133Xenon washout technique yielded a mean value of 8.0 cm3 X min-1 X 100 g tissue-1 whilst the microsphere technique gave a mean value of 7.8 cm3 X min-1 X 100 g tissue-1. There was a significant correlation (r = 0.83, p less than 0.006). In a further 14 rabbit thighs an attempt was made to measure skin blood flow by washout of 85Kr and to compare it with the microsphere technique. 133Xe promises to be a useful tracer for measuring lower limb flow when introduced intra-arterially. Because 85Kr tends to underestimate skin blood flow, and because of problems of diffusion of the tracer between compartments, which are discussed, this tracer is not satisfactory as a technique for measuring skin blood flow.
D000818:Animals; D006614:Hindlimb / Q000098:blood supply*; D007269:Injections, Intra-Arterial; D007726:Krypton; D008863:Microspheres; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D011868:Radioisotopes; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply*; D014980:Xenon Radioisotopes
D011868:Radioisotopes; D014980:Xenon Radioisotopes; D007726:Krypton