Partial purification and physicochemical properties of human T cell migration inhibitory factor (TIF).
Supernatants from 24 hr cultures of PHA-pulsed human T lymphocytes inhibit the migration of human peripheral blood T lymphocytes and guinea pig macrophages in vitro. The factor responsible for the inhibition of T lymphocytes provisionally called TIF (T cell migration inhibitory factor) was separated from MIF by preparative PAGE, had apparent molecular weight (m.w.) of 1,000-10,000 daltons and isoelectric point of 3.1. TIF activity was resistant to treatment with trypsin, chymotrypsin and neuraminidase but sensitive to PMSF (phenyl-methyl-sulfonyl-fluoride). This suggests that TIF is presumably different from human MIF and may represent a novel lymphokine which preferentially affects T cell migration in vitro.
D000818:Animals; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D007961:Leukocyte Migration-Inhibitory Factors / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D008222:Lymphokines / Q000302:isolation & purification*; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology*
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; C033370:neutrophil migration inhibitory factor