Inhibition of tissue-type plasminogen activator by conditioned medium from cultured human and porcine vascular endothelial cells.
Conditioned serum-free medium both from confluent porcine aortic endothelial cells and from human venous and arterial umbilical cord endothelial cells will inhibit plasminogen activation by tissue-type plasminogen activator. Inhibitory activity in the conditioned medium increases with time, and depends upon protein synthesis. Also, the conditioned medium directly inhibits the amidolytic activity of tissue-type plasminogen activator. Inhibitory activity can be removed from conditioned medium by absorption with immobilized tissue-type plasminogen activator.
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001808:Blood Vessels / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D004727:Endothelium / Q000378:metabolism; D005260:Female; D006801:Humans; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D013552:Swine; D014020:Tissue Extracts / Q000032:analysis; D014470:Umbilical Cord / Q000378:metabolism
D003470:Culture Media; D015849:Plasminogen Inactivators; D014020:Tissue Extracts; D010960:Plasminogen Activators