Pharmacokinetic studies on Ro 15-1788, a benzodiazepine receptor ligand, in the brain of the rat.
Methods for determining Ro 15-1788 in brain tissue were developed using gas chromatography with nitrogen-phosphorus detection, and using reverse-phase high performance liquid chromatography. Application of the methods to pharmacokinetic studies in the rat found the elimination half-life of Ro 15-1788 from rat brain to be 16 min. Ro 15-1788 was undetectable in rat plasma at the time points studied. Concentrations of Ro 15-1788 in the brain were reduced if chlordiazepoxide was coadministered.
D000818:Animals; D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002707:Chlordiazepoxide / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000032:analysis; D005442:Flumazenil; D006207:Half-Life; D007700:Kinetics; D008297:Male; D051381:Rats
D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D001569:Benzodiazepines; D005442:Flumazenil; D002707:Chlordiazepoxide; C015784:N-demethylchlordiazepoxide; C000869:demoxepam