Use of reducing compounds in the cultivation of Azospirillum sp.
Attempts were made to grow the micro-aerophilic N2-fixing bacterium Azospirillum sp. in complete liquid medium by incorporating some reducing agents. Ascorbic acid, glutathione and Na-thioglycollate stimulated while methylene blue inhibited the growth and N2-ase activity. In complete liquid medium, Na-thioglycollate and ascorbic acid increased the N2-ase activity with increasing concentration up to 800 micrograms/ml. With glutathione, growth of the bacterium was increased markedly but N2-ase activity was repressed below 200 micrograms/ml. The possibility of employing these compounds for the cultivation of Azospirillum in complete liquid medium seems to be indicated.
D001205:Ascorbic Acid / Q000494:pharmacology*; D001419:Bacteria / Q000201:enzymology / Q000254:growth & development*; D003470:Culture Media; D005978:Glutathione / Q000494:pharmacology*; D008751:Methylene Blue / Q000494:pharmacology; D009591:Nitrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D013864:Thioglycolates / Q000494:pharmacology*
D003470:Culture Media; D013864:Thioglycolates; D009591:Nitrogenase; D005978:Glutathione; D001205:Ascorbic Acid; D008751:Methylene Blue