A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.
D002262:Carboxy-Lyases / Q000235:genetics*; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000235:genetics*; D008828:Microbiological Techniques; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D005656:Fungal Proteins; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase