The amount of arachidonic acid in the triacylglycerols of perfused hamster lungs is increased by prednisolone.
Following the injection of 14C-arachidonic acid (4.1 nmol) into hamster isolated lungs about 80% of the administered radioactivity was retained by the lungs. During subsequent perfusion only a small amount of radioactivity was released to the perfusion effluent. This release was not affected by pulmonary infusion of prednisolone at 20 microM or 100 microM. In control lungs 84 +/- 1% (+/- SEM) of the retained radioactivity was recovered in the phospholipid, 13 +/- 1% in the neutral lipid and 3 +/- 1 in the free fatty acid fraction. Pulmonary infusion of prednisolone increased the amount of radiolabel in the neutral lipids. This was due to the increased amount of 14C-arachidonic acid in triacylglycerols. Prednisolone had no significant effects on the amount of 14C-arachidonate in diacylglycerols or in different phospholipids. Neither was the amount of free 14C-arachidonate in the lungs changed by prednisolone. The present study indicates that the release of arachidonic acid from triacylglycerols may be inhibited by prednisolone in hamster lungs.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis*; D006224:Cricetinae; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D008168:Lung / Q000032:analysis* / Q000187:drug effects; D008297:Male; D008647:Mesocricetus; D010477:Perfusion; D011239:Prednisolone / Q000494:pharmacology*; D014280:Triglycerides / Q000032:analysis*
D001095:Arachidonic Acids; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D016718:Arachidonic Acid; D011239:Prednisolone