[Natural variability of Streptomyces spheroides--a producer of extracellular proteolytic enzymes possessing fibrinolytic action].
Streptomyces spheroides M 8-2 was obtained using UV irradiation of spores taken from the parent culture. It shows a high activity of exocellular proteases which are capable of fibrin hydrolysis and degradation of blood clots. On certain media, a population of S. spheroides M8-2 was shown to yield three variants differing in their morphologo-biochemical properties. During submerged cultivation, these variants produce different quantities of exocellular proteases (3 to 19 units per 1 mg of biomass) with the fibrinolytic activity. Variants I and III are most active; their proteolytic activity is 10-14 units per 1 mg of biomass, on the average, and can reach 18-19 units per 1 mg of biomass. Variants with a low activity (variant II) are accumulated when the actinomycets is kept as spores on a solid medium with corn extract and on a solid medium with fibrin. The high proteolytic activity of the strain can be preserved by selection on a diagnostic medium with fibrin taking account of the diameter of hydrolytic zones around the colonies and selecting solely the colonies of variants I and III.
D003470:Culture Media / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D014644:Genetic Variation / Q000528:radiation effects; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000494:pharmacology / Q000528:radiation effects; D012641:Selection, Genetic; D013171:Spores, Bacterial / Q000201:enzymology / Q000528:radiation effects; D013302:Streptomyces / Q000201:enzymology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D003470:Culture Media; D005337:Fibrin; D010447:Peptide Hydrolases
Estestvannaia izmenchivost' Streptomyces spheroides--produtsenta vnekletochnykh proteoliticheskikh fermentov, obladaiushchikh fibrinoliticheskim deĭstviem.