Mechanism of protein excretion by gram-negative bacteria: Pseudomonas aeruginosa exotoxin A.
Excretion of proteins by a cell with a double membrane may involve mechanisms different from secretion across a single membrane. We studied this problem with Pseudomonas aeruginosa exotoxin A. This 68,000-dalton protein was released as rapidly as it was completed; even after short pulse-labeling the cells contained neither the toxin nor a larger precursor. Excretion is evidently cotranslational, since in fractionated lysates the toxin was formed (almost entirely in the mature form) by the membrane-polysome complexes but not by the free polysomes. When the membrane was perturbed by 10% ethanol, the cells stopped excreting the toxin and they accumulated an immunoprecipitable, enzymatically active precursor of 71,000 daltons. The precursor was located entirely in the outer membrane on its outer surface. On removal of the ethanol, the cells again excreted mature toxin, but they did not process or release the previously accumulated precursor. Based on these data, a model for the excretion of exotoxin A is presented.
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002462:Cell Membrane / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology; D005098:Exotoxins / Q000235:genetics* / Q000302:isolation & purification; D007700:Kinetics; D011132:Polyribosomes / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D000431:Ethanol; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A