Immunocytochemical staining of cervical smears for the diagnosis of cervical intraepithelial neoplasia.
A total of 233 cervical smears were stained by immunocytochemical methods for epithelial membrane antigen (EMA); the findings were compared with those from Papanicolaou-stained smears from the same women. Squamous epithelial cells from normal cervices did not stain, but cells shed from cervices with cervical intraepithelial neoplasia (CIN) did express the EMA marker. Metaplastic cells from normal and abnormal cervices also frequently stained. The results confirm that this marker detects cervical intraepithelial neoplasia in vitro, but its potential use in an automated screening program may be limited by the staining of the metaplastic cells.
D000951:Antigens, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology; D002584:Cervix Uteri / Q000276:immunology / Q000473:pathology; D004847:Epithelial Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D009367:Neoplasm Staging; D065006:Papanicolaou Test; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000473:pathology*; D014626:Vaginal Smears
D000951:Antigens, Neoplasm