Production of prostacyclin, 6-keto-PGF1 alpha and thromboxane B2 by incubated samples of umbilical vessels: a comparison of methods for expressing the results on dry weight, wet weight, protein or DNA bases.
The production of PGI2 (determined by bioassay), and of 6-keto-PGF1 alpha and TXB2 (determined by radioimmunoassay) by samples of human umbilical vessels have been measured. The results have been calculated on four bases: dry weight, wet weight, protein and DNA. There was a higher production of PGI2 and 6-keto-PGF1 alpha by umbilical veins than by umbilical arteries; no significant difference in TXB2 production was observed between umbilical veins and arteries. The ratio of 6-keto-PGF1 alpha: TXB2 production was about 100 for the samples of veins and about 40 for the samples of arteries. The best methods of expressing the results were on the bases of protein and DNA, the latter basis being marginally the best. The least satisfactory method for expressing the results was that based on dry weight. The physiological and practical implications of the results are discussed.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis* / Q000302:isolation & purification; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D011464:Epoprostenol / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D006801:Humans; D009928:Organ Specificity; D010974:Platelet Aggregation / Q000187:drug effects; D011247:Pregnancy; D011863:Radioimmunoassay; D013929:Thromboxane B2 / Q000096:biosynthesis* / Q000302:isolation & purification; D013931:Thromboxanes / Q000096:biosynthesis*; D014469:Umbilical Arteries / Q000378:metabolism*; D014471:Umbilical Veins / Q000378:metabolism*
D013931:Thromboxanes; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D000244:Adenosine Diphosphate; D011464:Epoprostenol