Identification of two distinct lactate dehydrogenases in Rhodospirillum rubrum.
The activities of pyridine nucleotide-independent d- and l-lactate dehydrogenases were detected in membranes from Rhodospirillum rubrum grown under aerobic and phototrophic conditions. Crossed immunoelectrophoretic analysis revealed two antigenically distinct enzymes that were further distinguished by specificity for d- and l-stereoisomers of lactate and by the sensitivity of the d-lactate dehydrogenase to inhibition by oxamate and oxalate.
D007123:Immunoelectrophoresis, Two-Dimensional; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D012247:Rhodospirillum rubrum / Q000201:enzymology*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase