Isolation of immunochemically distinct form of cytochrome P-450 from microsomes of tulip bulbs.
A highly purified cytochrome P-450 was obtained from the microsomes of tulip bulbs (Tulipa gesneriana L.). The molecular weight (Mr = 52,500) and amino acid composition of this plant cytochrome P-450 are similar to those reported for rat livers. On the contrary, Ouchterlony double diffusion analyses indicated that cytochrome P-450 isolated from tulip bulbs shares no common antigenic determinants with those of 9 other plants, in spite of the presence of comparable contents of cytochrome P-450 and/or trans-cinnamate 4-monooxygenase with tulip bulbs.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007106:Immune Sera; D005779:Immunodiffusion; D008861:Microsomes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity
D000596:Amino Acids; D007106:Immune Sera; D003577:Cytochrome P-450 Enzyme System