Freeze-fracture study of the rat parathyroid gland under hypo- and hypercalcemic conditions, with special reference to secretory granules.
Freeze-fracture images of the parenchymal cells in the parathyroid gland of rats were observed after vitamin D2 plus calcium chloride-suppression and EGTA-activation of secretion. In cells of the suppressed glands, large bulges protruded from the Golgi cisternae, and large granules with a stalk, which are identified as storage granules, suggest that, during maturation, some storage granules may be connected by long tubules with the Golgi cisternae and supplied with secretory products from the Golgi cisternae via these tubules. In the activated glands, presumptive exocytotic and endocytotic specializations of intramembranous particles of the parenchymal cell plasma membrane were frequently observed. In addition, elevations and complementary shallow depressions of various shape and extent were occasionally encountered in the intercellular space. From their morphological characteristics it was concluded that these originated from secretory granule cores, which are discharged from the parenchymal cells into the intercellular space by exocytosis, and it was suggested that discharged granule cores may retain their spherical shape until they fuse to form a flat conglomerate.
D000818:Animals; D002118:Calcium / Q000097:blood*; D002122:Calcium Chloride / Q000494:pharmacology; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004533:Egtazic Acid / Q000494:pharmacology; D004872:Ergocalciferols / Q000494:pharmacology; D005089:Exocytosis; D005110:Extracellular Space; D005614:Freeze Fracturing; D006056:Golgi Apparatus / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D010280:Parathyroid Glands / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains
D004872:Ergocalciferols; D004533:Egtazic Acid; D002122:Calcium Chloride; D002118:Calcium