Cytoplasmic transformation of Saccharomyces cerevisiae using purified mtDNA.
Transformation of cytoplasmic mutants of Saccharomyces cerevisiae proved to be successful using plasmid or intact mitochondria, but not when mtDNA was used as the transforming principle. The sphaeroplasts of two cytoplasmic mutants, respiratory deficient and erythromycin sensitive cells, were transformed using purified mtDNA from respiratory competent or erythromycin resistant donor strains.
D003593:Cytoplasm; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D008928:Mitochondria; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004272:DNA, Mitochondrial