Hybrid with rabbit skeletal DTNB-light chains of heavy meromyosin, myosin, and glycerinated fibers from Akazara scallop adductor.
It was shown in our previous report (Ojima et al. (1983) J. Biochem. 94, 307-310) that hybridization of Akazara scallop "desensitized" myosin with rabbit skeletal DTNB-light chains led to inhibition of the Mg-ATPase activity of acto-desensitized myosin but to enhancement of its superprecipitation activity. The following are now found: Development of tension in desensitized glycerinated fibers of Akazara adductor is significantly improved when DTNB-light chains are added to the fiber bath. The actin-affinity of desensitized heavy meromyosin in the presence of ATP but in the absence of Ca2+ is decreased by hybridization with chicken gizzard 20K dalton-light chains but significantly increased by that with DTNB-light chains. It is therefore suggested that the increase in actin-binding may account for the enhancing effect of DTNB-light chains on the superprecipitation and on the tension development.
D000818:Animals; D002645:Chickens; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology*; D005895:Gizzard, Avian / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology*; D007700:Kinetics; D008974:Mollusca; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D009579:Nitrobenzoates / Q000494:pharmacology*; D055503:Protein Multimerization; D011817:Rabbits
D015879:Myosin Subfragments; D009579:Nitrobenzoates; D004228:Dithionitrobenzoic Acid; D009218:Myosins; D005990:Glycerol