Enhanced survival of cultured cerebellar Purkinje cells by plating on antibody to Thy-1.
Survival of newborn mouse cerebellar Purkinje cells in dissociated monolayer cultures was substantially enhanced when the dissociated cells were plated on a substrate containing antibody to the Purkinje cell surface antigen Thy-1. Cells were identified by size, asymmetric arbors, uptake of gamma-aminobutyric acid (GABA), and failure to express glial fibrillary acidic protein. This selective enhancement was specific for antibody to the Thy-1.2 allele.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002470:Cell Survival; D002478:Cells, Cultured; D007518:Isoantibodies; D051379:Mice; D008807:Mice, Inbred BALB C; D008858:Microscopy, Phase-Contrast; D011689:Purkinje Cells / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D007518:Isoantibodies; C035678:anti-Thy antibody; D005680:gamma-Aminobutyric Acid