Effect of the thermal variation on the molecular weight estimation of the acid phosphatase from Physarum polycephalum.
A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and K strains. 28-31 degrees C is the critical temperature range for the breaking of enzyme-BP association in CL strains. This critical temperature is higher than 39 degrees C for the extract of CHR strains. CHR BP recognizes and binds CL acid phosphatase as strongly as CHR acid phosphatase. Km determination at 32 degrees C and thermoinactivation patterns at 66 degrees C indicate that the BP has no apparent regulatory role for the acid phosphatase activities. The presence or the absence of the BP in some of the strains may be used as a marker in the genetical studies of this organism.
D000135:Acid Phosphatase / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis; D007700:Kinetics; D008970:Molecular Weight; D010804:Physarum / Q000201:enzymology*; D013696:Temperature
D002352:Carrier Proteins; D000135:Acid Phosphatase