Human thymidine kinase. Purification and properties of the cytosolic enzyme of placenta.
Cytosolic thymidine kinase (EC 2.7.1.21) has been purified 5200-fold to apparent homogeneity from normal human placenta. The purification includes sequential affinity chromatography on blue-Sepharose and a thymidine column. The molecular weight of the enzyme determined by gel filtration and sucrose density ultracentrifugation is 92,000. The subunit molecular weight is 44,000, suggesting that the enzyme is a dimer in its native state. With isoelectric focusing, placental thymidine kinase demonstrated a single form with an isoelectric point of 9.1. The final purified enzyme preparation exhibits no immunological cross-reactivity with human mitochondrial thymidine kinase.
D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D046911:Macromolecular Substances; D008970:Molecular Weight; D009705:Nucleosides / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013937:Thymidine Kinase / Q000302:isolation & purification* / Q000378:metabolism
D046911:Macromolecular Substances; D009705:Nucleosides; D013937:Thymidine Kinase