DNA repair synthesis following exposure to chemical mutagens in primary liver, stomach, and intestinal cells isolated from rainbow trout.
DNA repair synthesis was autoradiographically measured in liver, stomach, and intestinal cells isolated from rainbow trout which were exposed in vitro to the chemical mutagens, N-methyl-N'-nitro-N-nitrosoguanidine, 4-nitroquinoline 1-oxide, and aflatoxin B1. The level of repair was greatest in primary hepatocytes which responded to all three mutagens. Only nominal amounts of repair were detected in stomach cells following N-methyl-N'-nitro-N-nitrosoguanidine and 4-nitroquinoline 1-oxide exposures and in intestinal cells following 4-nitroquinoline 1-oxide exposure. In comparison with cultured rainbow trout cells, the quantity of DNA repair found in primary cells is significantly less.
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D005753:Gastric Mucosa / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D009600:Nitroquinolines / Q000633:toxicity*; D009928:Organ Specificity; D013270:Stomach / Q000187:drug effects; D014337:Trout
D000348:Aflatoxins; D002273:Carcinogens; D009600:Nitroquinolines; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D016604:Aflatoxin B1