Vimentin intermediate filaments in cultures of human meningiomas.
Monolayer cultures of six human meningiomas and meningeal cells from a human foetus were examined by indirect immunofluorescence with a human autoantibody to intermediate filaments and with a monoclonal antibody to vimentin intermediate filaments. No difference could be demonstrated in the staining of an intricate fibrillar network in cultures of transitional, fibroblastic, psammomatous and sarcomatous meningiomas compared to those of human foetal meninges. Many meningotheliomatous meningioma cells showed staining of distinctive 'whorls' of intermediate filaments, an observation less frequently seen in fetal meningeal cells or in meningiomas of other histological types. Meningioma cells, pretreated with vinblastine, showed staining of rearranged filaments whose conformation and compactness varied from cell to cell. A striking observation frequently seen in transitional and psammomatous meningiomas was the staining of thick intermediate filament 'bands' bridging two contiguous meningioma cells. Immunoblotting experiments confirmed the presence of vimentin intermediate filaments in the cultured meningioma cells.
D002467:Cell Nucleus / Q000648:ultrastructure; D046508:Culture Techniques; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D008577:Meningeal Neoplasms / Q000473:pathology*; D008578:Meninges / Q000473:pathology; D008579:Meningioma / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology
D014746:Vimentin; D014747:Vinblastine