[Binding of tritiated corticosterone by brain sections of adrenalectomized rats].
In the present work we describe a new technique which permits us to study the specific binding of tritiated corticosterone in brain sections of adrenalectomized Rats. Under these conditions, the specific binding of the glucocorticoid represents 60 to 70% of the initial binding. The apparent dissociation constant and the number of binding sites, determined by Scatchard analysis, are in the range of 10(-8) M and 100 fmoles/mg of protein respectively.
D000315:Adrenalectomy; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003345:Corticosterone / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014316:Tritium
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; C024357:corticosterone receptor; D014316:Tritium; D003345:Corticosterone
Liaison de la corticostérone tritiée sur des coupes de cerveau de rat surrénalectomisé.