Electron spectroscopic imaging: parallel energy filtering and microanalysis in the fixed-beam electron microscope.
A new imaging modality in electron microscopy uses energy filtration to produce micrographs with elastically scattered electrons or with electrons that have lost a specific, often characteristic amount of energy in interacting with the specimen. No deleterious effects on microscope performance are encountered. Instead, microanalysis of specimens is made possible with a spatial resolution of 3 to 5 A and a sensitivity of detection of 2 X 10(-21) g corresponding to about 50 atoms of phosphorus. Elements detected range from hydrogen (Z = 1) to uranium (Z = 92). Examples of elemental mapping show membrane structure, DNA within nucleosomes, and RNA within ribosomal particles.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis; D004577:Electron Probe Microanalysis / Q000379:methods*; D004817:Epidermis / Q000032:analysis / Q000648:ultrastructure; D004926:Escherichia coli / Q000032:analysis / Q000648:ultrastructure; D008121:Nephropidae; D010758:Phosphorus / Q000032:analysis; D012270:Ribosomes / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis / Q000648:ultrastructure
D010758:Phosphorus; D004247:DNA