In vitro action of combinations of antimicrobial agents and EDTA-tromethamine on Pseudomonas aeruginosa.
Combinations of EDTA-tromethamine and 7 antimicrobial agents (chloramphenicol, nalidixic acid, oxytetracycline, penicillin, polymyxin-B, streptomycin, and triple sulfa) were tested for synergistic activities against Pseudomonas aeruginosa. Three in vitro tests were used, including minimal inhibitory concentrations of the drugs, a 2-dimensional Microtiter checkerboard technique, and bacterial inhibition studies. A synergistic inhibitory action was observed with combinations of EDTA-tromethamine plus penicillin and EDTA-tromethamine plus oxytetracycline. When chloramphenicol, streptomycin, nalidixic acid, polymyxin-B, or triple sulfa was mixed with EDTA-tromethamine, synergistic action did not occur.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004283:Dog Diseases / Q000188:drug therapy; D004285:Dogs; D004338:Drug Combinations; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004492:Edetic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D010032:Otitis Externa / Q000188:drug therapy / Q000662:veterinary; D010118:Oxytetracycline / Q000494:pharmacology; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000662:veterinary; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014325:Tromethamine / Q000494:pharmacology* / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D010406:Penicillins; D014325:Tromethamine; D004492:Edetic Acid; D010118:Oxytetracycline