A modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme A reductase.
A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts.
D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D007231:Infant, Newborn; D007700:Kinetics; D008297:Male; D011865:Radioisotope Dilution Technique; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012867:Skin / Q000201:enzymology
D002250:Carbon Radioisotopes; D006903:Hydroxymethylglutaryl CoA Reductases