Effect of different inhibitors on nonspecific alkaline phosphatase in the microsomal fraction from isolated odontoblasts.
In the microsomal fraction isolated from rat incisor odontoblasts, the influence of several inhibitors on p-nitrophenyl phosphatase activity (p-NPPase) was demonstrated at pH 10.3 and compared with the influence at pH 8.8. The inhibiting effect of EDTA on the p-NPPase activity was reversed by equimolar concentrations of Zn2+. The inhibitory effect of three diphosphonates, ethane-1-hydroxydiphosphonate (EHDP), methanediphosphonate (MDP), and dichloromethanediphosphonate (Cl2MDP) was equal to that of a chelating agent. Tetramisole and levamisole exerted an inhibitory effect that was different from the complexing type. L-Cystein, but not L-ascorbic acid, inhibited p-NPPase activity with a maximum inhibitory effect at pH 10.5.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004164:Diphosphonates / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D009804:Odontoblasts / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013773:Tetramisole / Q000031:analogs & derivatives / Q000378:metabolism
D004164:Diphosphonates; C006226:4-bromotetramisole; D004492:Edetic Acid; D013773:Tetramisole; D000469:Alkaline Phosphatase; D003545:Cysteine; D001205:Ascorbic Acid