Contractile activity of the isolated rat vas deferens and release of prostaglandin E and F-like substances. Influences of acetylcholine and inhibitors of cyclo-oxygenase and phospholipase A2.
The isolated epididymal portion of the rat vas deferens releases spontaneously and in response to acetylcholine prostaglandin-like material into the suspending solution. The output of prostaglandin E2 and F2 alpha-like substances, evoked by the cholinergic agonist, was significantly higher than the basal generation. Atropine, but not hexamethonium, markedly reduced the release elicited by acetylcholine. In addition corticosterone, quinacrine and verapamil also decreased significantly the acetylcholine-induced prostaglandin output. Inasmuch as quinacrine and corticosterone antagonized the response to acetylcholine the possible involvement of the cholinergic agonist on the activity of phospholipase A2, is suggested. Furthermore, the role of prostaglandins in relation to the action of acetylcholine on the contractile activity of the epididymal portion of isolated vas deferens, was also explored. It is concluded that these autacoids modulate the contractions evoked by acetylcholine.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D016861:Cyclooxygenase Inhibitors; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010276:Parasympatholytics / Q000494:pharmacology; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D011458:Prostaglandins E / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014649:Vas Deferens / Q000187:drug effects / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D010276:Parasympatholytics; D011458:Prostaglandins E; D011460:Prostaglandins F; D014700:Verapamil; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D000109:Acetylcholine