[Immunoenzyme analysis. Quantitative determination of antibodies to the influenza virus].
Solid-phase immunoenzymatic procedure for quantitative estimation of antibodies to virus of influenza A was developed. A relationship was found between the rate of influenza virus adsorption on polystyrene and concentration of the cells, duration of the adsorption, temperature and the nature of buffer systems. Optimal conditions were developed for binding of the virus with polystyrene, immune sera and the conjugate IgG-peroxidase. The suitable procedure is described for immunoenzymatic analysis in serologic diagnosis of influenza.
D000914:Antibodies, Viral / Q000032:analysis*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D009980:Influenza A virus / Q000276:immunology*; D013379:Substrate Specificity
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D006735:Horseradish Peroxidase
Immunofermentnyĭ analiz. Usloviia kolichestvennogo opredeleniia antitel k virusu grippa.