Field evaluation of ELISA using Wuchereria bancrofti mf ES antigen for bancroftian filariasis.
An enzyme-linked immunosorbent assay using Wuchereria bancrofti microfilarial excretory-secretory antigen was used in field studies to screen blood samples collected on filter-paper from persons residing in areas endemic for bancroftian filariasis. This assay system, when compared with examination of night wet blood smears for microfilariae, gave a relative sensitivity of 98% and a relative specificity of 86%. Daytime blood samples can also be used in this test, which can thus replace tedious examination of night blood samples in field surveys in endemic areas.
D000947:Antigens, Helminth; D001800:Blood Specimen Collection; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D014957:Wuchereria / Q000276:immunology*; D014958:Wuchereria bancrofti / Q000276:immunology*
D000947:Antigens, Helminth; C045193:Wuchereria antigen