Involvement of calcium ions in the activation of ornithine decarboxylase by isoprenaline evaluated 'in situ' in the perfused rat heart.
Ornithine decarboxylase activity evaluated during the perfusion of isolated rat hearts by a method 'in situ' was rapidly increased when the hearts were infused with isoprenaline (isoproterenol). Omission of Ca2+ from the perfusion medium or the administration of verapamil to the perfused hearts decreased the isoprenaline-stimulated ornithine decarboxylase activity, whereas a marked stimulatory effect was registered when the hearts were perfused with the Ca2+ ionophore A23187.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002262:Carboxy-Lyases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006321:Heart / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014700:Verapamil / Q000494:pharmacology
D000001:Calcimycin; D014700:Verapamil; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D007545:Isoproterenol; D002118:Calcium