[New substances with high specific activity for nucleic acid sequences: oligodeoxynucleotides covalently linked to an intercalating agent].
New molecules are described in which an intercalating agent is covalently linked through a polymethylene chain to an oligodeoxynucleotide. Using an oligodT sequence covalently linked to an acridine dye, it is shown that these molecules bind in a specific way to their complementary sequence (poly (A] and that the stability is strongly enhanced by the intercalating agent as compared with the unmodified oligodT. Substitution of a phosphate group by a positively charged group strengthens complex formation and protects the neighboring phosphodiester bond against nuclease cleavage.
D007364:Intercalating Agents / Q000378:metabolism*; D008956:Models, Chemical; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D009994:Osmolar Concentration
D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C027903:oligo (dT)
Nouvelles substances à forte affinité spécifique pour des séquences d'acides nucléiques: oligodésoxynucléotides liés de façon covalente à un agent intercalant.