The determination of chlorophylls a and b together with 14CO2 fixation in the same plant tissue samples.
A simple, efficient, and inexpensive method for measuring radioactivity as well as chlorophylls a and b in a large number of plant tissue samples is presented. Chlorophyll is determined following extraction with dimethyl sulfoxide or N-N'-dimethylformamide. The solvent is then evaporated on glass-fiber filters and bleached under light. The filter disks are counted together with the cleared plant material.
D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002734:Chlorophyll / Q000302:isolation & purification; D008722:Methods; D010944:Plants / Q000032:analysis / Q000378:metabolism*
D002250:Carbon Radioisotopes; D002734:Chlorophyll; D002245:Carbon Dioxide