Some factors affecting the quantitation of rheumatoid factors by enzyme immunoassay.
Some factors affecting the use of enzyme-labelled antibody to human IgM or enzyme-labelled human IgG for the detection and assay of IgM RFs by enzyme immunoassay have been investigated. IgM RF levels, obtained by standard radioimmunoassay techniques, showed a better agreement with those obtained with enzyme-labelled antibody than with enzyme-labelled human IgG. Immuno-adsorption studies using a sheep cell agglutination test (SCAT) confirmed a partial loss of antigenicity of the IgG molecules due to glutaraldehyde treatment, which increased with the length of time of exposure to glutaraldehyde.
D000469:Alkaline Phosphatase; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001666:Binding Sites, Antibody; D004306:Dose-Response Relationship, Immunologic; D005976:Glutaral; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D012217:Rheumatoid Factor / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor; D000469:Alkaline Phosphatase; D005976:Glutaral