A rapid method for the determination of naringin, prunin, and naringenin applied to the assay of naringinase.
A naringinase assay capable of distinguishing between the content of naringin, prunin, and naringenin present in the incubation mixture, is described. The amount of these compounds can be estimated by combining two spectrophotometric procedures. (a) Treatment with strong alkali to determine the amount of nargingenin as well as the sum of naringin and prunin. (b) Assay of the liberated aldohexoses with o-aminodiphenyl. From the data thus obtained, the amount of the remaining substrate, the amount of the intermediate as well as the product at any given time can be calculated.
D001234:Aspergillus niger / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis*; D005916:Globulins; D005959:Glucosidases / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000032:analysis*; D009097:Multienzyme Complexes / Q000032:analysis*; D010940:Plant Proteins; D013053:Spectrophotometry / Q000379:methods; D001617:beta-Glucosidase / Q000032:analysis*
D044950:Flavanones; D005419:Flavonoids; D005916:Globulins; D009097:Multienzyme Complexes; D010940:Plant Proteins; C039828:prunin protein, Prunus; D005959:Glucosidases; D006026:Glycoside Hydrolases; C039827:naringinase; D001617:beta-Glucosidase; C005273:naringenin; C005274:naringin