Effect of human lymphoblastoid interferon on insulin synthesis and secretion in isolated human pancreatic islets.
Human islets of Langerhans were isolated from the pancreas removed from a 13-year-old female transplant donor. The islets were incubated in a culture medium for 24 h in the presence of human lymphoblastoid interferon (1000 units/ml). Insulin secretion, proinsulin biosynthesis, total protein biosynthesis and total insulin content were assessed at various concentrations of glucose in the presence of interferon. In interferon-treated islets glucose-stimulated insulin secretion was unaltered from that of control islets; however, glucose-stimulated proinsulin biosynthesis was specifically inhibited by interferon (48%, p less than 0.025). Total protein biosynthesis and total insulin content were not significantly affected by interferon.
D005947:Glucose / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis* / Q000378:metabolism; D000078790:Insulin Secretion; D007370:Interferon Type I / Q000502:physiology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D011384:Proinsulin / Q000096:biosynthesis; D014316:Tritium
D007328:Insulin; D007370:Interferon Type I; D014316:Tritium; D010649:Phenylalanine; D011384:Proinsulin; D005947:Glucose