Specific deletion occurring in the directed evolution of 6-phosphogluconate dehydrogenase in Escherichia coli.
A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS5) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.
D001426:Bacterial Proteins / Q000235:genetics*; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics*; D012641:Selection, Genetic
D001426:Bacterial Proteins; D010734:Phosphogluconate Dehydrogenase