The presence of EC collagen and type IV collagen in bovine Descemet's membranes.
When bovine Descemet's membranes (DMs) were characterized after limited pepsinization the major component in DM was found to be endothelial cell (EC) collagen. Phenol extractions of the undigested pepsin residue recovered only type IV collagen. This study provides evidence that EC collagen may be produced by corneal endothelial cells in vivo.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D002417:Cattle; D002855:Chromatography, Thin Layer; D003094:Collagen / Q000032:analysis*; D003315:Cornea / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K; D010450:Endopeptidases / Q000378:metabolism; D004727:Endothelium / Q000032:analysis; D005260:Female; D006801:Humans; D010434:Pepsin A / Q000378:metabolism; D011247:Pregnancy; D012756:Sheep
D000596:Amino Acids; D003094:Collagen; D010450:Endopeptidases; D019286:Endopeptidase K; D010434:Pepsin A