Augmentation of B-cell responsiveness by a tumor-activated T-cell factor.
The growth of the P815 mastocytoma in syngeneic DBA/2 mice led to an activation of Ly1+2- T cells. These T cells produced a soluble factor or factors in culture which, when added to normal spleen cells or B cells in the presence of syngeneic Ly1 cells, caused a genetically unrestricted augmentation of the plaque-forming response toward sheep red blood cells (SRBC). The culture supernatant of the activated T cells did not support the proliferation of an interleukin-2 (IL-2)-dependent cell, nor exhibit properties of late-acting TRF. Active supernatants appeared to affect directly B cells during the first 48 hr of culture with SRBC in such a way as to make them more responsive to antigen-specific Ly1-cell help.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001798:Blood Proteins / Q000502:physiology*; D003208:Concanavalin A / Q000096:biosynthesis; D006462:Hemolytic Plaque Technique; D007376:Interleukin-2 / Q000096:biosynthesis; D015848:Interleukin-5; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000096:biosynthesis; D008297:Male; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D001798:Blood Proteins; D007376:Interleukin-2; D015848:Interleukin-5; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; C011850:immunoenhancing factor; D003208:Concanavalin A