Myocardial protection during cardiac ischemia by coronary perfusion with cold lactated Ringer's solution plus mannitol.
This experimental and clinical study evaluates the degree of myocardial protection afforded by coronary perfusion with cold lactated Ringer's solution plus mannitol. In the experimental study, diluted blood at 30 degrees C (Control G.), lactated Ringer's solution at 4 degrees C (G. A), 1,000 ml lactated Ringer's solution plus 200 ml of 20% mannitol at 4 degrees C (G. B), and 1,000 ml lactated Ringer's solution plus 500 ml of 20% mannitol at 4 degrees C (G. C) were used for coronary perfusion. The myocardial-protecting effect of each perfusate was evaluated by examining serum isoenzymes (CPK-MB, LDH1+2), left ventricular function (Vmax, LVEDP, CO), and the ultrastructure of myocardium. there were no significant differences in myocardial changes between the control group, G. A and G. B. Left ventricular enddiastolic pressure increased significantly in G. A and a significant increase in CPK-MB was seen in G. C. Mitochondrial scores for each group were 98 (Control G.), 86 (G. A), 93 (G. B), and 51 (G. C). Minimal myocardial change was seen in G. B. In the clinical study aortic root perfusion with cold lactated Ringer' s solution (400 m Osm) under aortic cross clamping (27-144 min) was employed in 25 patients. They all survived and CPK-MB returned to preoperative values within 49 hours after operation. In 6 cases, the ultrastructure of left ventricular papillary muscle was examined. It was confirmed that no significant mitochondrial changes developed during aortic cross-clamping.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003080:Cold Temperature; D003326:Coronary Circulation; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D006324:Heart Arrest, Induced; D006801:Humans; D007527:Isoenzymes; D007552:Isotonic Solutions / Q000008:administration & dosage; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008353:Mannitol / Q000008:administration & dosage*; D009206:Myocardium / Q000473:pathology; D010477:Perfusion; D000077325:Ringer's Lactate
D007527:Isoenzymes; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D008353:Mannitol; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase