Aroclor 1254 pretreatment enhances the DNA repair response to amino acid pyrolysate mutagens in primary cultures of rat hepatocytes.
The stimulation of unscheduled DNA synthesis (UDS) by 3-amino-1,4-dimethyl -5H-pyrido[4,3-b]indole (Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) and 2-amino-dipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) was measured in primary cultures of rat hepatocytes. Hepatocellular DNA was recovered as a cetyltrimethylammonium salt from nuclei digested with protease. The incorporation of [3H]thymidine was measured by liquid scintillation counting techniques. Although Trp-P-1 was the most potent of the pyrolysis products tested, it was considerably less active than 2-aminofluorene (2-AF) or aflatoxin B1 (AFB1). A greater induction of UDS by Trp-P-1 was observed in cultures from female rats than in cultures from male rats. Aroclor 1254 pretreatment resulted in a substantially greater UDS response to all 4 pyrolysis products.
D000818:Animals; D001140:Aroclors / Q000494:pharmacology*; D002243:Carbolines / Q000494:pharmacology*; D002478:Cells, Cultured; D020111:Chlorodiphenyl (54% Chlorine); D004260:DNA Repair; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D001140:Aroclors; D002243:Carbolines; D007093:Imidazoles; D007211:Indoles; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine); C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D011078:Polychlorinated Biphenyls; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; C023847:Glu-P-2