A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes.
NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this single step procedure is as pure as that isolated by multicolumn procedures.
D000818:Animals; D002846:Chromatography, Affinity; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase