[Membrane-active properties of a preparation from a bacterial culture broth possessing autoregulation action].
The effect of a preparation obtained by butanol extraction of the culture fluid of B. cereus and Ps. carboxydoflava and previously termed an autoregulatory factor d1 on the respiration chain within intact bacterial cells and isolated membranes was investigated. In comparable concentrations this factor d1 inhibits the endogenous respiration of B. cereus and M. lysodeikticus as well as oxidase and dehydrogenase activities of isolated membranes from these bacteria and E. coli. The factor-induced decrease of the cell respiration rate is independent from disorders of the cell permeability osmotic barrier for respiration substrates. The factor d1 shows a higher effect at acidic pH values. It is concluded that the above preparation has membrane-active properties. It is also suggested that in the bacterial cell its target is the inner surface of the cytoplasmic membrane.
D001409:Bacillus cereus / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects*; D003470:Culture Media / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects*; D008837:Micrococcus / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D011549:Pseudomonas / Q000502:physiology*
D003470:Culture Media; D010100:Oxygen
Membranoaktivnye svoĭstva preparata iz kul'tural'noĭ zhidkosti bakteriĭ, obladaiushchego autoreguliatornym deĭstviem.