A computer-based data analysis system for enzyme-linked immunosorbent assays.
A computerized system is presented for automating the data collection, processing, and displaying tasks involved in enzyme-linked immunosorbent assays. This system uses a through-the-well absorbance reader of microtiter plates interfaced to a minicomputer running the UNIX operating system. Optical density in each well of a 96-well microtiter plate is recorded as a function of time for up to 10 time points. These data are automatically transmitted to the remote computer. The rate of product formation is then calculated for each well, and a battery of analysis, display, and comparison programs can then be used by the researcher for data presentation. Using the initial rate of reaction as the basis for quantifying enzyme-linked immunosorbent assays focuses on the catalytic property of the enzyme and allows a large dynamic range of the assay on any plate. These programs can be adapted to virtually any mini- or microcomputer with a graphics display or a plotting device. Assuming moderately powerful computing hardware, throughputs of 50 plates per day are easily achieved. The programs work equally well with peroxidase, beta-galactosidase, or alkaline phosphatase conjugated second antibodies, and with whole cell or soluble antigens.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D003201:Computers / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D008951:MNSs Blood-Group System / Q000276:immunology; D008905:Minicomputers; D012984:Software / Q000295:instrumentation* / Q000379:methods; D001616:beta-Galactosidase
D000911:Antibodies, Monoclonal; D008951:MNSs Blood-Group System; D001616:beta-Galactosidase