Competitive gut exclusion of avian pathogens by Lactobacillus acidophilus in gnotobiotic chicks.
A total of 205 Grey Leghorn chicks were hatched germfree for separate trials to determine the effects of Lactobacillus acidophilus treatment on pathogenic Salmonella typhimurium and Staphlococcus aureus. Prophylactic and therapeutic treatments with L. acidophilus were administered either before or after the pathogens were introduced. Prophylactic treatments significantly reduced chick mortality (P less than .01) and shedding of the pathogens (P less than .05). The L. acidophilus prophylactic treatments were also effective qualitatively in reducing the isolation of S. typhimurium and Staph. aureus from crop contents but not, to a great extent, from cecal or rectal contents of gnotobiotic chicks at postmortem. The average surface pH values for the crop, proventriculus, duodenum, cecum, and rectum for gnotobiotic chicks were 5.43, 5.02, 6.18, 6.56, and 6.71, respectively. The L. acidophilus treatments did not significantly affect surface pH of the various segments of the intestinal tract.
D000818:Animals; D000898:Antibiosis; D002645:Chickens / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005856:Germ-Free Life; D007422:Intestines / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000254:growth & development*; D009026:Mortality; D012486:Salmonella typhimurium / Q000254:growth & development; D013211:Staphylococcus aureus / Q000254:growth & development