Acetylcholine measurement by high-performance liquid chromatography using an enzyme-loaded postcolumn reactor.
Choline oxidase and cholinesterase were found to retain their activity for 1-2 weeks at room temperature while adsorbed to a commercially available anion-exchange cartridge. These enzymes convert acetylcholine to H2O2. Acetylcholine can be measured in tissue extracts by separation at pH 7 on a polymeric reverse-phase high-performance liquid chromatography column, conversion of acetylcholine to H2O2 on a postcolumn enzyme-loaded anion-exchange cartridge, and electrochemical detection of the H2O2 formed.
D000109:Acetylcholine / Q000032:analysis*; D000429:Alcohol Oxidoreductases; D000818:Animals; D000837:Anion Exchange Resins; D001923:Brain Chemistry; D002794:Choline / Q000032:analysis; D002802:Cholinesterases; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized; D051379:Mice; D051381:Rats; D012469:Salivary Glands / Q000032:analysis; D012822:Silicon Dioxide
D000837:Anion Exchange Resins; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; C029349:SynChropak AX-300; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002802:Cholinesterases; D002794:Choline; D000109:Acetylcholine