[Effect of the physiological conditions on alpha-amylase and glucoamylase formation by a selected strain of Aspergillus oryzae].
The production of alpha-amylase and glucoamylase by a selected strain of Aspergillus oryzae was investigated using different carbon and nitrogen sources. The best and most economic fermentation medium for the production of the both amylases in submerged cultures had the following composition (in %): defatted rice brain, 8; corn steep liquor, 3; MgSO4 X 7H2O, 0.1; KH2PO4, 0.1; CaCl2, 0.1. The optimum pH was 5.0. The optimal conditions for biosynthesis of the amylases were as follows: cultivation at 28 degrees C for 96 h using the 0.5% mycelial suspension as an inoculum.
D001230:Aspergillus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D003470:Culture Media / Q000378:metabolism; D004790:Enzyme Induction; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D005959:Glucosidases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D012641:Selection, Genetic; D013696:Temperature; D013997:Time Factors; D000516:alpha-Amylases / Q000096:biosynthesis*
D003470:Culture Media; D005959:Glucosidases; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase
Vliianie fiziologicheskikh usloviĭ na obazovanie al'fa-amilazy i gliukoamilazy selektsionirovannym shtammom Aspergillus oryzae.