UDP-galactose:ceramide galactosyl transferase of isolated oligodendroglia.
The activity of UDP-galactose:ceramide galactosyl transferase (CGalT) has been studied in isolated oligodendroglia from bovine brain white matter and myelinating rat brain. The specific activity and activity per mg DNA are 4- and 10-fold higher in rat oligodendroglia compared with neuronal perikarya from rat brain, and is higher in oligodendroglia from myelinating rat brain compared with bovine oligodendroglia. In membranes isolated from oligodendroglia, the specific activity decreased in the order endoplasmic reticulum greater than plasma membrane greater than myelin.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D002469:Cell Separation; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005700:Galactosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D051147:Ganglioside Galactosyltransferase; D066298:In Vitro Techniques; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D009836:Oligodendroglia / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology
D005700:Galactosyltransferases; D051147:Ganglioside Galactosyltransferase