Elicitation and detection of in vitro H-2-linked Ir gene regulated antibody responses to poly(LTyr, Glu)-poly(DLAla)--poly(LLys).
A microculture system is described in which secreted antibody responses to the synthetic polypeptide (T,G)-A--L were obtained in vitro. Responses were highly reproducible, antigen-dependent, antigen-specific, and under H-2-linked Ir gene control. Critical elements in the system include the schedule of in vivo antigen-priming, removal of the stimulating antigen after 3 days of culture, and a sensitive detection system (double-antibody ELISA). This system should be useful in the analysis of the mechanism of action of Ir genes as well as the mechanisms by which anti-idiotype antibodies modulate immune responses.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D010455:Peptides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D006183:H-2 Antigens; D007075:Immunoglobulin M; D010455:Peptides; C021759:(T,G)-A-L