Liquid chromatographic determination of mitomycin C in human plasma and urine.
A method is given for the determination of the antineoplastic drug mitomycin C in plasma and urine samples. Mitomycin is isolated from the biological matrix with the aid of a Sep-Pak C18 extraction column and eluted with methanol. The methanol is evaporated and the residue is redissolved in the chromatographic mobile phase (methanolic phosphate buffer). Mitomycin C is separated from coextracted compounds by reversed-phase liquid chromatography on a LiChrosorb RP-8 column. A high detection sensitivity and selectivity was obtained by photometric measurements at 365 nm. The precision of the determinations was better than 6% relative standard deviation for plasma samples within the range 2-1000 ng/ml, and for urine samples within the range 0.5-4.4 micrograms/ml. The pH-dependent stability of mitomycin in buffer solutions has been studied.
D002021:Buffers; D002853:Chromatography, Liquid; D006207:Half-Life; D006801:Humans; D007700:Kinetics; D016685:Mitomycin; D008937:Mitomycins / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D013053:Spectrophotometry; D013696:Temperature
D002021:Buffers; D008937:Mitomycins; D016685:Mitomycin