Hormonal regulation of pig Leydig cells in culture.
Using a primary culture of purified immature pig Leydig cells, the hormonal and non hormonal regulation of steroidogenic activity have been studied. The effects of LH/hCG, FSH, LHRH, o-prolactin, estradiol, epidermal growth factor (EGF), human low density lipoprotein (hLDL) and human high density lipoprotein (hHDL) on 125I-hCG binding and basal and hCG-stimulated cAMP and steroids (testosterone and dehydroepiandrosterone sulfate) production were evaluated. These data on the regulation of pig Leydig cell activity are compared with other published results, mainly in the rat model, obtained either in vivo and in vitro.
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007985:Leydig Cells / Q000187:drug effects / Q000502:physiology*; D008074:Lipoproteins / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D011388:Prolactin / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D006728:Hormones; D008074:Lipoproteins; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D004815:Epidermal Growth Factor; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone