[Experiments with Bacillus thuringiensis protoplasts. II. Study of the potential interspecies fusion of bacterial protoplasts: Bacillus thuringiensis and Bacillus megaterium].
In the present study the possibility of obtaining interspecific bacterial hybrids by polyethylene glycol-assisted fusion of protoplasts from Bacillus thuringiensis and Bac. megaterium has been examined. Electron microscopic and genetic data allow to confirm with great probability that cytological fusion takes place. However, genetic analysis revealed that neither of methods applied for protoplast fusion gave stable recombinants. Apparently, it is due to the lack of recombination or the death of recombinants that follows the functioning of the cell correction system. The mechanism of protoplast fusion and its most important steps are also studied in the present work.
D001410:Bacillus megaterium / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D005838:Genotype; D008854:Microscopy, Electron; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects / Q000648:ultrastructure*; D011995:Recombination, Genetic / Q000187:drug effects; D013045:Species Specificity
D011092:Polyethylene Glycols
Eksperimenty s protoplastami Bacillus thuringiensis. Soobshchenie II. Issledovanie vozmozhnosti mezhvidovogo sliianiia bakterial'nykh protoplastov: Bacillus thuringiensis i Bacillus megaterium.