Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells.
A mononuclear cell chemoattractant of high specific activity produced by baboon (Papio cynocephalus) aortic medial smooth-muscle cells (SMCs) in culture has been partially characterized. Smooth-muscle cells, between the third and eighth passage, were grown to confluence in Medium 199 containing 10% fetal calf serum and then incubated for 24 hours in either serumless medium (Neuman and Tytell) or Medium 199 containing 0.2% bovine serum albumin. The 24-hour SMC-conditioned medium was fractionated on Sephadex G100-Superfine and potent chemoattractant activity (SMC-CF) eluted in the 10,000-12,000 dalton region. SMC-CF displayed chemotactic and chemokinetic activity for peripheral blood mononuclear cells but not for polymorphonuclear leukocytes. Production of SMC-CF by the cells was significantly inhibited in the presence of cycloheximide, and its activity was abolished after incubation with the bacterial protease subtilisin. Chromatofocusing experiments indicate that SMC-CF is a cationic protein with a pI of greater than 10.5. The role of SMC-CF may play as an inflammatory mediator in monocyte recruitment to the arterial intima in atherogenesis is discussed.
D000818:Animals; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000302:isolation & purification* / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D016209:Interleukin-8; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism* / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology; D010215:Papio; D010447:Peptide Hydrolases / Q000494:pharmacology
D002630:Chemotactic Factors; D016209:Interleukin-8; D003513:Cycloheximide; D010447:Peptide Hydrolases