Bioluminescence assay of calcium with a liquid scintillation counter.
The luminescence produced by Ca aequorin reaction in dilute solutions decays exponentially over a relatively prolonged period of time. The concentration of total Ca and of free Ca in Ca-EGTA buffers could be determined by measuring the decay of luminescence in a liquid scintillation counter. The method is also suitable for determining total Ca concentration in small tissue samples.
D000331:Aequorin; D000818:Animals; D001011:Aorta / Q000032:analysis*; D002118:Calcium / Q000032:analysis*; D004533:Egtazic Acid; D005260:Female; D008163:Luminescent Measurements; D008722:Methods; D011817:Rabbits; D012588:Scintillation Counting; D014599:Uterus / Q000032:analysis*
D000331:Aequorin; D004533:Egtazic Acid; D002118:Calcium