Accumulation of IgG and complement factor C3 in human arterial endothelium and atherosclerotic lesions.
Endothelial injury is associated with an intracellular accumulation of IgG. Fluorescent antibodies were used to detect IgG and complement factor C3 in biopsies of human arteries. Both proteins were found in endothelial cells with signs of injury, in normal and atherosclerotic arteries. Rhodamine-conjugated anti-IgG and FITC-conjugated anti-C3 were used to count the number of IgG- and C3-containing endothelial cells in non-atherosclerotic cystic arteries. IgG was found in 26% of the cells, with C3 in 38% of the IgG-containing cells. Both IgG and C3 were also observed in the subendothelial intima of cystic arteries and in atherosclerotic lesions of iliac and femoral arteries. The accumulation of IgG and C3 in injured endothelium and in atherosclerotic lesions could be of importance for complement-mediated tissue destruction and for the attraction and activation of white blood cells.
D000328:Adult; D000368:Aged; D001158:Arteries / Q000276:immunology; D001161:Arteriosclerosis / Q000276:immunology*; D003176:Complement C3 / Q000032:analysis*; D004727:Endothelium / Q000276:immunology; D005263:Femoral Artery / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005704:Gallbladder / Q000098:blood supply; D006801:Humans; D007083:Iliac Artery / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D066298:In Vitro Techniques; D008875:Middle Aged
D003176:Complement C3; D007074:Immunoglobulin G