[Changes in the membrane fluidity of human neutrophilic granulocytes under the effect of a chemoattractant (FMLP) and of echo virus, type 9, A. Barty strain].
Preincubation of human polymorphonuclear leucocytes (PMNs) with different concentrations of Echo virus, type 9, strain A. Barty results in viral dose- and time-dependent inhibition of chemotactic cellular response to chemoattractant (N-formylmethionylleucylphenylalanine = FMLP). In the present experiments, by means of biophysical methods using excimer forming lipids (pyrendecanoic acid) the influence of FMLP and Echo 9 virus on membrane fluidity of PMNs was investigated. It is shown that the increase of membrane fluidity elicited by FMLP is reduced by the virus in a dose dependent manner. These results indicate virus-induced disturbance of molecular architecture and failure of natural signal processing of PMN-membranes. This biophysical method, therefore, can be used as a functional histochemical method to demonstrate functional defects in membranes of living cells.
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004459:Echovirus 9 / Q000378:metabolism*; D029822:Enterovirus B, Human / Q000378:metabolism*; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D008715:Methionine / Q000031:analogs & derivatives*; D009239:N-Formylmethionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000382:microbiology; D009842:Oligopeptides / Q000494:pharmacology*
D009842:Oligopeptides; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine
Veränderungen der Membranfluidität menschlicher neutrophiler Granulozyten unter dem Einfluss eines Chemoattractant (FMLP) und von Echo-Virus, Typ 9, Stamm A. Barty.