Dipyridamole reduces the motility of 10T1/2 cells and disturbs their stress fibre pattern.
Dipyridamole (DPD), a widely used anti-thrombotic agent, inhibits in vitro directional migration of 10T1/2 mouse embryo cells in a dose dependent way. The inhibition is detectable at DPD concentrations comparable to those in human plasma after oral intake of the drug. Using a model for quantitative shape change analysis, it is demonstrated that DPD inhibits pseudopodal activity and stabilizes the outlines of 10T1/2 cells. The inhibition of pseudopodal activity is thought to be involved in the reduced migratory capacity and the decreased area occupied by DPD treated cells. Coomassie blue staining of detergent-extracted cells shows that stress fibre patterns in 10T1/2 cells are altered by DPD. In particular, highly regular, polygonal networks, reminiscent of those described in spreading cells, appear after DPD treatment. It is suggested that interference of DPD with the normal organization of the actin microfilament system accounts for its effects on spreading, motility, and growth of cultured cells, and possibly for part of its in vivo anti-thrombotic activity as well.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004176:Dipyridamole / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008857:Microscopy, Interference; D008858:Microscopy, Phase-Contrast
D004176:Dipyridamole