Glycogen synthesis in resident and thioglycollate-elicited rat peritoneal exudate cells.
The values of the A0.5 for glucose-6-P, apparent Km for UDPglucose and -/+glucose-6-P activity ratio are similar for glycogen synthase derived from rat resident and thioglycollate-elicited peritoneal macrophages; the specific activity is 7-fold higher for the enzyme from thioglycollate-elicited macrophages. The rate of incorporation of [14C]glucose into macrophage glycogen is 7-fold greater in the elicited population that that in the resident one; the values of the S0.5 for glucose are similar. The in vitro activation of glycogen synthase proceeds at a greater rate and extent for the enzyme from elicited macrophages; thus, phosphatase activity may be reduced in resident macrophages relative to that in thioglycollate-elicited ones.
D000818:Animals; D001202:Ascitic Fluid; D001711:Biotransformation; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis*; D006006:Glycogen Synthase / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051381:Rats; D013864:Thioglycolates / Q000494:pharmacology*
D013864:Thioglycolates; D006003:Glycogen; D006006:Glycogen Synthase; D005947:Glucose