Effect of lipophilic vitamins on the erythrocyte membrane. 31P NMR and fluorescence studies.
Structural changes induced by the fat-soluble vitamins A, D3, E and K1 in natural membranes were studied by 31P NMR and fluorescence anisotropy on erythrocyte ghosts; the occurrence of cell fusion following vitamin addition was detected by optical microscopy of intact cells. Vitamins A, E and K1, which produce cell fusion, also induce the formation of configurational phases other than the bilayer and increase, at a different extent for the different vitamins, membrane fluidity. Vitamin D3, on the contrary, induces aggregation without fusion, paralleled by bilayer phase stabilization and microviscosity increase. A correlation between fusion phenomena and structural variations in the bilayer organization is therefore suggested.
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects; D002645:Chickens; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity / Q000187:drug effects; D012995:Solubility; D014815:Vitamins / Q000494:pharmacology*
D008055:Lipids; D014815:Vitamins