Binding of staphylococcal enterotoxin A (SEA) with human splenic lymphocytes.
1. The presence of specific binding of [125I]SEA with human splenocytes is established. 2. The association of toxin at 4 C is characterized by saturation, reversibility and a great affinity to a receptor (Kd = 4.0 x 10(-7) M). 3. The number of binding sites on a cell is equal to 6000. 4. At 23 C the binding of labelled toxin with a cell described by a biphasic curve. 5. Priming increases the association of SEA with the splenocytes and correspondingly increases the production level of gamma-interferon.
D004768:Enterotoxins / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008214:Lymphocytes / Q000378:metabolism; D011485:Protein Binding; D013154:Spleen / Q000378:metabolism*; D013210:Staphylococcus / Q000378:metabolism*
D004768:Enterotoxins; D007371:Interferon-gamma