Fate of collagen-tailed acetylcholinesterase upon extraction of chick retina with salt-detergent solutions in the absence of EDTA.
In chick retina, the tailed 20S molecular form of acetylcholinesterase (A12) is slowly degraded to globular forms after homogenization of the tissue in a buffer-salt-detergent solution, in the absence of EDTA. This process can be stopped by the addition of EDTA to the homogenate, prior to high speed centrifugation; however, longer delays in adding EDTA lead to lower recoveries of tailed enzyme. The rate of degradation of A12 is considerably enhanced by high speed centrifugation of this EDTA-less homogenate prior to addition of EDTA.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D002498:Centrifugation / Q000379:methods; D002645:Chickens; D003902:Detergents; D004492:Edetic Acid / Q000494:pharmacology; D012160:Retina / Q000201:enzymology*; D012965:Sodium Chloride; D013997:Time Factors; D014020:Tissue Extracts / Q000302:isolation & purification
D002021:Buffers; D003902:Detergents; D014020:Tissue Extracts; D012965:Sodium Chloride; D004492:Edetic Acid; D000110:Acetylcholinesterase