Immunohistochemical typing of amyloid on hydroxyethyl-methacrylate-embedded renal biopsies.
Hydroxyethyl-methacrylate (GMA)-embedded renal biopsies containing amyloid were tested by the indirect immunoperoxidase method with antibodies against the following purified amyloid fibril proteins: AA, three different A lambda preparations, Ak and AF. The anti-AA reagent was monoclonal (mc13), all the others were polyclonal. In four biopsies from cases with generalized amyloidosis, two were found to be of the AA and two of the A lambda type. Two control cases of membranous glomerulonephritis showed only a marginal reaction with anti-A lambda. These results demonstrate that GMA-embedded tissue sections are suitable for immunohistochemical classification of amyloid diseases, because proteinaceous antigenic determinants of amyloid fibril are preserved.
D000368:Aged; D000682:Amyloid / Q000145:classification / Q000378:metabolism*; D000686:Amyloidosis / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007674:Kidney Diseases / Q000378:metabolism*; D008297:Male; D008689:Methacrylates; D008875:Middle Aged; D000685:Serum Amyloid A Protein / Q000145:classification / Q000378:metabolism
D000682:Amyloid; D008689:Methacrylates; D000685:Serum Amyloid A Protein; C005044:hydroxyethyl methacrylate