A simple and efficient method for the production of human gamma interferon.
A method for the production of human gamma interferon (HuIFN-gamma), which is both simple and efficient, has been developed. The 2 co-inducers, A-23187 and mezerein, stimulate human peripheral blood lymphocytes to produce high levels of HuIFN-gamma in both stationary cultures and spinner cultures. The compound 2,2-dimethyl-6,6,7,7,8,8,8-heptafluoro-3,5-octanedione (FOD) has also proven to be a useful co-inducer. The production of HuIFN-gamma appears to occur in at least 2 phases.
D000643:Ammonium Chloride / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004224:Diterpenes; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology; D007158:Immunologic Techniques; D007369:Interferon Inducers / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D010703:Phorbol Esters / Q000494:pharmacology; D013729:Terpenes; D005466:Fluorocarbons
D000643:Ammonium Chloride; D000001:Calcimycin; D004224:Diterpenes; D006845:Hydrocarbons, Fluorinated; D007369:Interferon Inducers; D007371:Interferon-gamma; D010703:Phorbol Esters; D013729:Terpenes; C029279:6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octanedione; D005466:Fluorocarbons; C011309:mezerein