A simple method for collecting 14CO2 in fish.
A simple apparatus was designed for continuous collection of excreted 14CO2 in fish. Carp (Cyprinus carpio; body wt. about 400 g) were intraarterially injected with [14C]acetate (44 mmol) and transferred to a test chamber (21 +/- 1 degrees C). Within 48 h, 65.4 +/- 2.7% of the dose was recovered as 14CO2. A small amount of 14CO2 was retained in the water of the test chamber. Control experiments using [14C]acetate-injected mice as '14CO2-generators' showed that this 14C residue in the water could be recovered almost completely as 14CO2 upon acidification. In the carp experiments, some radioactivity remained in the acidified test chamber water; this residue may represent unchanged [14C]acetate and/or metabolites other than 14CO2.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000032:analysis; D002347:Carps; D007269:Injections, Intra-Arterial; D008722:Methods; D051379:Mice; D014867:Water / Q000032:analysis
D000085:Acetates; D002250:Carbon Radioisotopes; D014867:Water; D002245:Carbon Dioxide; D019342:Acetic Acid