Increased immunofluorescent staining of rabies-infected, formalin-fixed brain tissue after pepsin and trypsin digestion.
This study was undertaken to evaluate the sensitivity of the direct immunofluorescence test on Formalin-fixed, trypsin-digested, rabies-infected brain tissue. Our results suggest that the optimal unmasking of rabies antigenic sites is obtained by using a double enzyme digestion with pepsin and trypsin in lieu of only trypsin.
D000818:Animals; D001921:Brain / Q000382:microbiology / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D051379:Mice; D010434:Pepsin A / Q000378:metabolism*; D011818:Rabies / Q000473:pathology*; D014357:Trypsin / Q000378:metabolism*
D005557:Formaldehyde; D014357:Trypsin; D010434:Pepsin A