Sequence-specific DNA modification in Neisseria gonorrhoeae.
Neisseria gonorrhoeae 82409/55(pJD1) is postulated to possess six DNA sequence-specific cytosine methyltransferases and one DNA sequence-specific N6-adenine methyltransferase. From the DNA sequencing of the plasmid pJD1 (manuscript in preparation) by a modification of the Maxam and Gilbert chemical cleavage procedure, the cytosine methylation specificities were demonstrated. Five of these methylating enzymes and their respective specificities are M . NgoI (formula; see text) does not methylate the cytosine of its recognition sequence, in agreement with a detected adenine modification. A biological implication of these different DNA methylating activities is discussed.
D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D010957:Plasmids; D013379:Substrate Specificity
D004269:DNA, Bacterial; D008780:Methyltransferases