[Effect of roentgen irradiation on the luminescent properties of rat liver lysyl-tRNA synthetase].
The parameters of proper fluorescence of lysil-tRNA-synthetase from rat liver were determined in normal conditions and after X-irradiation. Irradiation of animals with a minimal absolutely lethal dose (0.21 C/kg) caused conformational transformations of the protein molecule accompanied by a change in the microenvironment of the chromophores: 60 min after irradiation it became less polar and after 24 h more polar than normal. The changes in the conformational status of the enzyme molecules after irradiation were connected with a change in the specific activity of lysil-tRNA-synthetase.
D000604:Amino Acyl-tRNA Synthetases / Q000528:radiation effects*; D000818:Animals; D008099:Liver / Q000201:enzymology* / Q000528:radiation effects; D008163:Luminescent Measurements; D008250:Lysine-tRNA Ligase / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects; D051381:Rats; D014916:Whole-Body Irradiation
D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase
Vliianie rentgenovskogo oblucheniia na liuminestsentnye svoĭstva lizil-tRNK-sintetazy iz pecheni krys.