[Effect of somatomedin A and its serum cofactor on human fibroblasts in culture. Relation between mitogenic activity and effects on the biosynthesis of collagen and other proteins].
It was demonstrated in a previous paper that the sulfation activity of somatomedin A (SM-A) depends on the presence of a low molecular weight cofactor (CoF) present in human serum. In this paper, the effects of the two factors on human dermis fibroblast proliferation and syntheses of proteins and collagen were studied. The addition of SM-A alone to the culture medium had no mitogenic effect but modified the distribution of newly synthesized proteins, since 14C-proline incorporation was increased into proteins of the cell layer and decreased into proteins of the medium. The addition of CoF alone had no significant effect. Simultaneous addition of SM-A and CoF restarted cell divisions and increased the inhibiting activity of SM-A on the secretion of proteins into cell supernatant.
D002453:Cell Cycle; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007335:Insulin-Like Growth Factor II; D007700:Kinetics; D008934:Mitogens; D014176:Protein Biosynthesis; D013002:Somatomedins / Q000302:isolation & purification / Q000502:physiology*
D008934:Mitogens; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II; D003094:Collagen
Effets de la somatomédine A et de son cofacteur sérique sur les fibroblastes humains en culture. Relations entre activité mitogène et effets sur la biosynthèse du collagène et des autres protéines.