Glycosyltransferase activities and the differentiation of human promyelocytic (HL60) cells by retinoic acid and a phorbol ester.
The activities of five glycosyltransferases were measured following the induction of HL60 cells to differentiate to mature myeloid forms or to macrophages by the addition of retinoic acid or a phorbol ester, respectively. Gal-T-II, Fuc-T-I and (NeuAc-T-I) are all increased and Fuc-T-II decreased in activity upon treatment with RA. Gal-T-I and Fuc-T-II are decreased and Gal-T-II increased in activity upon with TPA treatment. The increases in enzyme activities with RA are measurable as early as 1 day but while Fuc-T-I and NeuAc-T-I are fully elevated at 2 days, Gal-T-II shows a biphasic rise with initial elevation by day 2 and a further rise at days 3 to 5. The rises in Gal-T-II are due to increases in the enzyme form present in uninduced cells.
D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005647:Fucosyltransferases / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000503:physiopathology*; D009439:Neuraminidase / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
D010704:Phorbols; D014212:Tretinoin; D005647:Fucosyltransferases; D005700:Galactosyltransferases; D006602:Hexosyltransferases; D009439:Neuraminidase; D013755:Tetradecanoylphorbol Acetate