[Morphological study of the organs and tissues of mice infected after a burn with a Pseudomonas aeruginosa strain and treated with a Pseudomonas aeruginosa polyvalent corpuscular vaccine and tobramycin].
The effect of tobramycin and polyvalent corpuscular Ps. aeruginosa vaccine on the infectious process in mice with grade III burns inoculated with Ps. aeruginosa 1312 was studied. The highest percentage of the survival (100 per cent) among the animals was observed, when the vaccine was applied locally every day for 7 days. With the use of tobramycin administered intramuscularly for 2 times 95 per cent of the animals survived. When the vaccine was administered subcutaneously, 96.6 per cent of the animals survived. Morphological investigation of the organs and tissues of the mice showed that the vaccine applications to the infected burns promoted rapid elimination of microorganisms in the wounds and prevented development of sepsis due to Ps. aeruginosa. At the same time early epithelization of the wounds was observed (by the 4th-7th day). Intramuscular injections of tobramycin and subcutaneous injection of vaccine also prevented development of sepsis due to Ps. aeruginosa and protected the animals from death. Still no epithelization of the wounds by that period was observed. Microscopic examinations revealed necrosis of the epiderma and derma elements and microbial swarms on the skin surface.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002056:Burns / Q000150:complications* / Q000473:pathology / Q000628:therapy; D004353:Drug Evaluation, Preclinical; D006651:Histocytochemistry; D051379:Mice; D011552:Pseudomonas Infections / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013997:Time Factors; D014031:Tobramycin / Q000627:therapeutic use*
D000900:Anti-Bacterial Agents; D001428:Bacterial Vaccines; D014031:Tobramycin
Morfologicheskoe izuchenie organov i tkaneĭ mysheĭ, infitsirovannykh posle ozgoga shtammom sinegnoinoĭ palochki i lechennykh sinegnoĭnoĭ polivalentnoĭ korpuskuliarnoĭ vaktsinoĭ i tobramitsinom.