Two methods to induce 6-thioguanine resistance in human fibroblasts in the presence of rat-liver microsomes.
7,12-Dimethylbenz[a]anthracene and 4-aminobiphenyl induced dose-dependent 6-thioguanine-resistant mutations in normal human fibroblasts in the presence of rat S9 microsomes. With each chemical, mutations were induced by two different methods: single treatment of synchronized cells, and multiple treatment of nonsynchronized cells. Multiple treatment of nonsynchronized cells gave higher mutation frequencies at lower doses, and appears to be the more sensitive method.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000611:Aminobiphenyl Compounds / Q000494:pharmacology; D001711:Biotransformation; D002455:Cell Division; D002460:Cell Line; D003584:Cytological Techniques; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008862:Microsomes, Liver / Q000201:enzymology; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation / Q000187:drug effects*; D013866:Thioguanine / Q000494:pharmacology*
D000611:Aminobiphenyl Compounds; C006757:4-biphenylamine; D015127:9,10-Dimethyl-1,2-benzanthracene; D013866:Thioguanine