Glycosylated A alpha chains in chicken fibrinogen.
Fibrinogen immunoprecipitated from cultured chick embryo hepatocytes was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compared with fibrinogen from chicken plasma. The character and relatedness of the constituent polypeptide bands were established on the basis of enzymatic treatment, peptide analysis, metabolic labeling with [14C]glucosamine, and inhibition of glycosylation with tunicamycin. Hepatocyte-derived fibrinogen resolved into five polypeptides that, in order of decreasing apparent molecular weight, were identified as glycosylated A alpha, non-glycosylated A alpha, B beta, gamma', and gamma. Fibrinogen immunoprecipitated directly from chicken plasma yielded an identical profile except for an additional smaller A alpha chain. This small A alpha chain appears to be the product of partial proteolysis in the circulation and was the only A alpha band found in purified plasma fibrinogen (fraction I-2). The observation of glycosylated A alpha chains is novel. The gamma/gamma' chain heterogeneity appears to be due to an amino acid extension similar to that observed in mammalian fibrinogens. Fibrinogen from cells exposed to fetal bovine serum, a potent stimulator of fibrinogen production, was enriched in glycosylated A alpha chains, which constituted approximately one-third of the A alpha chain population. Serum did not affect the gamma/gamma' chain distribution.
D000818:Animals; D012103:Batroxobin / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D008099:Liver / Q000032:analysis / Q000196:embryology; D010446:Peptide Fragments / Q000302:isolation & purification; D013917:Thrombin / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology; D006023:Glycoproteins
D012103:Batroxobin; D005340:Fibrinogen; D010446:Peptide Fragments; D013917:Thrombin; D014415:Tunicamycin; C044548:glycosylated fibrinogen; D006023:Glycoproteins