The effect of defocusing on the electron microscopic image of the extended particle of pyocin R1.
The effect of defocusing on the electron microscopic image of the extended particle of pyocin R1 was examined with the aid of optical diffraction. The results show that the through-focus image of the cross-striated particle changed to a fishbone-like image with a little under-focusing, which had been considered to be a transitional state during the sheath contraction of phage G. In the optical diffraction pattern of the defocused image, a strong meridional reflection corresponding to the distance of cross-striations in the through-focus image almost disappeared, supporting the change to the fishbone-like image on defocusing.
D001430:Bacteriocins / Q000302:isolation & purification; D001435:Bacteriophages / Q000032:analysis; D008854:Microscopy, Electron; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000032:analysis; D011709:Pyocins / Q000302:isolation & purification
D001430:Bacteriocins; D011709:Pyocins