Experimental urinary tract infection in rabbits by a group D Streptococcus sp.: a study of serum immunoglobulin classes by enzyme-linked immunosorbent assay.
After induction of pyelonephritis and cystitis in rabbits by a group D streptococcus, the authors measured the specific serum antibody response in the three major immunoglobulin classes. The enzyme-linked immunosorbent assay was performed using group D streptococcal antigen extracted by phenol. Before experimentation, all rabbits had low IgM, IgG and IgA titres against group D streptococcal antigen. A marked increase in IgM antibodies was detected during the first month of experimental pyelonephritis. A poor IgM antibody response was observed in rabbits with cystitis. During the three months of experimentation, a significant difference in mean IgG levels was observed between rabbits with pyelonephritis and those with cystitis.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D003556:Cystitis / Q000175:diagnosis / Q000276:immunology*; D013293:Enterococcus faecalis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D011704:Pyelonephritis / Q000175:diagnosis / Q000276:immunology*; D011817:Rabbits; D013290:Streptococcal Infections / Q000175:diagnosis / Q000276:immunology*
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins