Properties of a monovalent and divalent requiring endoribonuclease with novel specificity from calf heart.
A new monovalent and divalent requiring endoribonuclease has been identified in extracts of calf heart tissue using a natural substrate molecule. This ribonuclease creates 5' hydroxyl and 3' phosphate groups in its cleavage products. It cleaves predominantly between pyrimidine and A residues though not all such possible sites are attacked in the substrates tested.
D000818:Animals; D001483:Base Sequence; D002413:Cations, Divalent; D002414:Cations, Monovalent; D002417:Cattle; D004722:Endoribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D006163:Ribonuclease T1 / Q000378:metabolism; D013379:Substrate Specificity
D002413:Cations, Divalent; D002414:Cations, Monovalent; D009843:Oligoribonucleotides; D004722:Endoribonucleases; C038406:ribonuclease CH; D006163:Ribonuclease T1