Enzyme-linked immunosorbent assay for determination of thyroglobulin autoantibodies. Comparison with RIA and haemagglutination.
An enzyme immunoassay (ELISA) has been developed for the detection of human autoantibodies to thyroglobulin (TgAb). The ELISA assay was compared to the previous routine method, the tanned red cell (TRC) method and a radioimmunoassay (RIA) technique for thyroglobulin autoantibodies. Sera from 163 healthy non-hospitalized persons, 31 patients with Hashimoto's thyroiditis, 13 patients with Graves disease, 136 patients with thyroid cancer and 365 sera consecutively received for thyroid autoantibody screening were examined for TgAb in the three methods. The nosological sensitivity for ELISA methods was comparable to that of RIA and higher than that of TRC. The ELISA method was accurate, precise, objective, inexpensive and well suited for quantitative routine determination of TgAb.
D001323:Autoantibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006111:Graves Disease / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D013954:Thyroglobulin / Q000276:immunology*; D013964:Thyroid Neoplasms / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000276:immunology
D001323:Autoantibodies; D013954:Thyroglobulin