[Role of membrane sialic acid of human granulocytes in the stimulation of NADPH,H+ oxidase activity].
The treatment of human polymorphonuclear cells by neuraminidase "type-X" removes about 15% of cell sialic acid without modifications of NADPH oxidase activity of granulocytes before and after stimulation by opsonized zymosan. A mild periodate treatment oxidizes only the poly-hydroxilic chain of sialic acid with formation of aldehyde groups. This treatment increases cellular NADPH oxidase activity and also largely prevents the stimulation of polymorphonuclear cells by opsonized zymosan.
D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D019255:NADPH Oxidases; D009439:Neuraminidase / Q000494:pharmacology*; D009504:Neutrophils / Q000201:enzymology*; D012794:Sialic Acids / Q000097:blood*; D015054:Zymosan / Q000494:pharmacology
D012794:Sialic Acids; D015054:Zymosan; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D009439:Neuraminidase
Rôle de l'acide sialique membranaire des granulocytes humains dans la stimulation de l'activité NADPH,H+ oxydase.