Application of HPLC measurement of plasma concentration of gabexate mesilate.
The plasma concentration of gabexate mesilate (ethyl-p-(6-guanidinohexanoyloxy) benzoate) methanesulfonate (GM), FOY, was measured on a high performance liquid chromatography. GM concentrations ranging from 1 to 50 micrograms/ml of whole blood were able to be determined with this method. The half-life of GM in plasma was 55 seconds and the minimum concentration of GM which affected the activated coagulation time of whole blood (ACT) was 10 micrograms/ml. This method is sensitive and accurate to provide us with the pharmacodynamic basis for the determination of the effective plasma concentration of GM with respect to its anticoagulant activity.
D000925:Anticoagulants; D002851:Chromatography, High Pressure Liquid; D016670:Gabexate; D006146:Guanidines / Q000097:blood* / Q000494:pharmacology; D006207:Half-Life; D006801:Humans; D011480:Protease Inhibitors / Q000097:blood*; D014914:Whole Blood Coagulation Time
D000925:Anticoagulants; D006146:Guanidines; D011480:Protease Inhibitors; D016670:Gabexate