Narcissus mosaic virus: a potexvirus with an encapsidated subgenomic messenger RNA for coat protein.
Narcissus-mosaic-virus RNA is translated into a coat-protein-sized product in wheat-germ cell-free extracts. This protein was shown to be very similar to authentic coat protein by partial proteolysis in SDS/polyacrylamide-gel electrophoresis, and by serology. Fractionation of the RNA revealed a small RNA molecule of approx. 840 nucleotides, which alone coded for the coat protein. This subgenomic RNA was found to be encapsidated in a short virus particle.
D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D014908:Triticum; D014764:Viral Proteins / Q000096:biosynthesis*
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins