Isolation of a branched chain alpha-keto acid dehydrogenase from rabbit liver.
An enzyme catalysing a series of reactions resulting in the oxidative decarboxylation of branched chain alpha-keto acids and production of NADH, was extracted from rabbit liver mitochondria with the aid of NaClO4. Purification yielded a product which appeared homogeneous on electrophoresis. The enzyme is active on three substrates alpha-ketoisocaproate, alpha-keto-beta-methyl valerate, and alpha-ketoisovalerate.
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D002417:Cattle; D013293:Enterococcus faecalis / Q000201:enzymology; D007658:Ketone Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D009097:Multienzyme Complexes; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)