Construction of recombination-deficient strains of Pseudomonas aeruginosa.
The rec-102 mutation had pleiotropic effects in Pseudomonas aeruginosa: low recombinational proficiency in conjugation and transduction; high UV sensitivity; inability to induce pyocin R2 by mitomycin C; and increased susceptibility to mitomycin C and nalidixic acid. The rec-102 locus was mapped by R68.45-mediated conjugation in the 45 min region of the PAO chromosome, between argF and thr-9001. By selection for a marker in this region, rec-102 can be introduced into a P. aeruginosa strain of interest using an R68.45 rec-102 donor. The recombination-deficient strains constructed in this way were phenotypically similar to Escherichia coli recA mutants.
D002874:Chromosome Mapping; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D011709:Pyocins / Q000096:biosynthesis; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D008937:Mitomycins; D011709:Pyocins; D016685:Mitomycin