B-cell differentiation antigen expressed on a human myeloma cell line (KMM-1).
KMM-1 is a human multiple myeloma cell line previously shown to express plasma cell phenotypes. Hybridomas against this cell line were produced by fusing spleen cells from hyperimmunized BALB/c mice with X63-Ag8-653 mouse myeloma cells. One monoclonal antibody derived from this fusion, termed 33-3, reacted with all the B-cell stages except the pre-B-cell stage. Both myeloma cells from patients and those from KMM-1 cells were reactive at the same maximum dilution of this antibody. No reactivity was seen with cells of T-lymphocyte origin, platelets, red blood cells, monocytes or granulocytes. The 33-3 antibody was unable to detect any specific marker distinguishing cultured myeloma cells from those that could not be cultured in vitro.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface