[Antibodies against p-aminophenyl-beta-D-galactopyranoside-containing proteins].
The antibodies were prepared from antisera of rabbits immunized with bovine serum albumin containing covalently bound p-aminophenyl-beta-D-galactopyranoside (APG) and purified by affinity chromatography on APG-containing ovalbumin immobilized by BrCN-activated Sepharose 4B. The antibodies possessed a selective specificity for APG and interacted with different APG-containing proteins, including APG-containing lysosomal alpha-glucosidase. The purified antibodies are immunoglobulins of G type as was determined from the molecular weights of native and dissociated antibodies and from the immunochemical assays with antibodies against rabbit IgG.
D000818:Animals; D000906:Antibodies; D002417:Cattle; D005697:Galactosides; D006027:Glycosides; D005779:Immunodiffusion; D008247:Lysosomes / Q000201:enzymology; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology*; D000520:alpha-Glucosidases / Q000276:immunology
D000906:Antibodies; D005697:Galactosides; D006027:Glycosides; D011506:Proteins; D012709:Serum Albumin; C010677:4-aminophenyl-beta-galactoside; D000520:alpha-Glucosidases
Antitela k p-aminofenil-beta-D-galaktopiranozidsoderzhashchim belkam.