Specific cellular immunity in acoustic neuroma patients.
An indirect leukocyte migration agarose technique to detect cell-mediated immunity was modified to obtain a specific assay for release of human leukocyte migration inhibitory factor. Acoustic neuroma patients exhibited a significant cellular immune response against acoustic neuroma extract (P less than .01) as well as perilymph from acoustic neuroma patients (P less than .01) when compared to healthy control persons. All 19 patients tested reacted to acoustic neuroma extract. Seven of 21 perilymph samples did not elicit migration inhibition. Crossover determination of antigenicity of two negative and four positive perilymph samples against three patients revealed highly reproducible results, uncorrelated to perilymph concentration of potassium and protein. Flow cytofluorometry did not reveal malignant DNA patterns in 10 acoustic neuromas examined. Immunofluorescence studies did not reveal autoantibodies against acoustic neuromas in sera from 11 patients. The responsible antigen(s), the mechanism of immunization, and the diagnostic implications remain to be determined.
D000328:Adult; D000368:Aged; D000418:Albumins / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000032:analysis; D007961:Leukocyte Migration-Inhibitory Factors / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000276:immunology*; D010498:Perilymph / Q000276:immunology
D000418:Albumins; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D007136:Immunoglobulins; D007961:Leukocyte Migration-Inhibitory Factors; D003165:Complement System Proteins