[Molecular profile of mitogen-active metabolites of Staphylococcus aureus].
A fraction with a molecular weight of 16000-24000 was isolated from 5-day S. aureus broth culture grown in the dialysate of Martin-Hottinger broth. At a concentration of 2-15 micrograms/ml this fraction induced the nonspecific blast transformation of human blood lymphocytes with the stimulation index 99.6 +/- 15.2 (72-hour cultures). The mitogenic fraction was immunochemically inert and unrelated to the main part of biologically active components, resistant to heating at 100 degrees C for 10 minutes and to lyophilization, inactivated (by 84.2%-73.7%) by pronase and trypsin, not inactivated by specific immunosorption. Mitogenically active metabolites are regarded as a separate type of biologically active S. aureus products.
D000328:Adult; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003378:Counterimmunoelectrophoresis; D006801:Humans; D005779:Immunodiffusion; D007163:Immunosorbent Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D008970:Molecular Weight; D013211:Staphylococcus aureus / Q000378:metabolism*
D008934:Mitogens
Molekuliarnyĭ profil' mitogenaktivnykh metabolitov zolotistogo stafilokokka.