Interaction of Fab mu of anti-galactocerebroside antibody with galactocerebroside liposomes.
The binding of Fab mu of rabbit anti-galactocerebroside antibody to antigen incorporated in unilamellar liposomes has been investigated by fluorescence and circular dichroism measurements. The fluorescence intensity of Fab mu is found to be quenched considerably in the presence of liposomes concomitant with a blue-shift of the emission maximum. It is inferred from circular dichroism studies that there are changes in the aromatic residues and the disulfides as well as in the folding of peptide backbone of Fab mu. The results suggest that antigen-associated liposomal membrane induces changes in both the tertiary and the secondary structure of Fab mu.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002554:Cerebrosides / Q000276:immunology*; D002942:Circular Dichroism; D005699:Galactosylceramides / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000032:analysis / Q000276:immunology*; D008081:Liposomes / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits
D000906:Antibodies; D002554:Cerebrosides; D005699:Galactosylceramides; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains; D008081:Liposomes; C002454:galactocerebroside