Endoglycosidase H-sensitive glycopeptides in eleven different animal cells.
We have examined the distribution of mannose-labelled glycopeptides in eleven different animal cells grown in vitro. All of the cells examined contained endoglycosidase H-sensitive species of high and low molecular weight, associated with the cell material and with the cell surface; however, the distribution between the two pools was different, suggesting a 'sorting out' of glycoproteins. Another conclusion from our studies is that the oligosaccharide processing known to occur during or after membrane glycoprotein translation is incomplete in a high percentage of mannose-containing N-linked oligosaccharides of cell surfaces. There was no consistent correlation between the relative amounts of endoglycosidase H-sensitive and -resistant glycopeptides and whether the cells were normal, virus-transformed or tumour-derived.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D002850:Chromatography, Gel; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D000882:Haplorhini; D006801:Humans; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D051379:Mice; D013045:Species Specificity
D006020:Glycopeptides; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase