[Cloning in Bacillus subtilis cells of the Bacillus mesentericus genes that determine the enzyme synthesis of tryptophan metabolism].
In this work, we tried to clone some Bacillus mesentericus genes coding for tryptophan synthesis in Bacillus subtilis cells. We succeeded in obtaining two identical plasmids carrying some genes of Bac. mesentericus and complementing the trpC2 and trpF mutations of Bac. subtilis. The cloned genes of Bac. mesentericus completely replaced the functions of trpC2 and trpF genes.
D019747:Aldose-Ketose Isomerases; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D002238:Carbohydrate Epimerases / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D005798:Genes, Bacterial; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D009876:Operon; D010957:Plasmids; D014364:Tryptophan / Q000378:metabolism*
D014364:Tryptophan; D007209:Indole-3-Glycerol-Phosphate Synthase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C029865:phosphoribosylanthranilate isomerase
Klonirovanie v kletkakh Bacillus subtilis nekotorykh genov Bacillus mesentericus, determiniruiushchikh sintez fermentov metabolizma triptofana.