Influence of certain prostaglandin synthetase inhibitors on cytoplasmic estrogen receptors in the uterus.
Prostaglandin synthetase inhibitors are widely used in the treatment of primary dysmenorrhea. They are thought to act by inhibiting endometrial synthesis of prostaglandins and subsequently altering endometrial receptor concentration. Since prostaglandins are known to vary with the menstrual cycle in women, implicating an effect associated with sex steroid hormones, we examined the influence of certain prostaglandin synthetase inhibitors on specific estrogen binding capacity of uteri from rats. Ibuprofen (Motrin), 25 mg/kg, indomethacin (Indocin), 15 mg/kg, mefenamic acid (Ponstel), 25 mg/kg, and sulindac (Clinoril), 40 mg/kg, were evaluated at 6 and 24 hours after injection. Specific estrogen-binding capacity was elevated twofold by mefenamic acid; the other agents showed no statistically significant effect. No alteration in the dissociation constant values of the estrogen receptors was observed with these agents. These data suggest that prostaglandin synthetase inhibitors do not influence the estrogen response mechanism in rodents.
D000818:Animals; D001665:Binding Sites; D016861:Cyclooxygenase Inhibitors; D003593:Cytoplasm / Q000378:metabolism*; D005260:Female; D007052:Ibuprofen / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008528:Mefenamic Acid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000187:drug effects*; D013467:Sulindac / Q000494:pharmacology; D013997:Time Factors; D014599:Uterus / Q000378:metabolism*
D016861:Cyclooxygenase Inhibitors; D011960:Receptors, Estrogen; D013467:Sulindac; D008528:Mefenamic Acid; D007052:Ibuprofen; D007213:Indomethacin