[Standardization of the nutrient medium for determining the biological activity of a number of antibiotics by the diffusion-in-agar method].
The possibility of using 2 variants of a unified nutrient medium containing no Hottinger's broth for determination of the biological activity of a large number of antibacterial antibiotics was shown. The medium provided satisfactory reproducibility of the results. It contained yeast extract as a source of nitrogen. The medium was selected with the method of the fractional factor experiment. Clear inhibition growth zones and the slope of the dose-response curve were used as the criteria for estimation of the optimal medium. The described nutrient medium allowed a 2-time increase in the sensitivity of the method used for determination of the biological activity of neomycin, monomycin, streptomycin, oxytetracycline, chlortetracycline and erythromycin.
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D003470:Culture Media / Q000592:standards*; D004058:Diffusion; D008826:Microbial Sensitivity Tests / Q000379:methods*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D000362:Agar
Unifikatsiia pitatel'noĭ sredy dlia opredeleniia biologicheskoĭ aktivnosti riada antibiotikov metodom diffuzii v agar.