[Inhibiting action of anti-arrhythmia agents of the phenothiazine series--etmozin and its diethylamino analog--on platelet aggregation and metabolism of endogenous arachidonic acid].
Effect of the cardiotropic drugs of the phenothiazine series ethmozine, and its diethylamine analogue (DAAE), on platelet aggregation and formation of arachidonic acid metabolites has been studied. Both drugs inhibit the ADP-induced aggregation in the platelet-rich plasma. Ethmozine inhibits only the second (irreversible) wave of aggregation, while DAAE inhibits both the first (reversible) and the second one. 50% inhibition (ID50) of the second wave of aggregation is observed at the following concentrations of the two agents: 300-500 micrograms/ml (ethmozine) and 20 micrograms/ml (DAAE). DAAE completely inhibits the irreversible aggregation of platelets washed off plasma, induced by arachidonic acid (ID50 approximately 30 micrograms/ml) and Ca2+-ionophore A23187 (ID approximately 55 micrograms/ml); the aggregation, induced by thrombin is inhibited by 80-90% (ID approximately 130 micrograms/ml). Formation of arachidonic acid metabolites in platelets effected by these inducers was measured by the accumulation of malondialdehyde (MDA). DAAE fails to inhibit MDA formation induced by exogenous arachidonic acid, but completely prevents the synthesis of MDA induced by A23187 and thrombin. These data suggest that DAAE inhibits the release of endogenous arachidonic acid from membrane phospholipids catalysed by phospholipase A2, but does not affect its subsequent metabolic transformations. In all probability, ethmozine and DAAE, just as other phenothiazines, affect platelets via the inhibition of Ca2+-calmodulin-dependent reactions and processes.
D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors / Q000097:blood*; D000001:Calcimycin / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008315:Malondialdehyde / Q000097:blood; D016293:Moricizine / Q000031:analogs & derivatives; D010640:Phenothiazines / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*
D000889:Anti-Arrhythmia Agents; D001095:Arachidonic Acids; D010640:Phenothiazines; C027240:diethylamino-ethmozine; D016718:Arachidonic Acid; D016293:Moricizine; D000001:Calcimycin; D008315:Malondialdehyde; D000244:Adenosine Diphosphate
Ingibiruiuschee deĭstvie antiaritmicheskikh preparatov fenotiazinovogo riada--étmozina i ego diétilaminovogo analoga--na agregatsiiu trombotsitov i metabolizm éndogennoĭ arakhidonovoĭ kisloty.