The met-enkephalin analog FK 33-824 and naloxone do not directly influence cortisol secretion by cultured human adrenocortical cells.
Systemic administration of the enkephalin analog FK 33.824 was previously shown to inhibit ACTH secretion in man. In this study, the direct action of this analog on cortisol release was studied. The enkephalin analog (1 microM and 10 microM) did not influence basal or ACTH-stimulated cortisol production by cultured isolated adrenocortical cells prepared from the hyperplastic adrenal glands from three patients with Cushing's disease. Naloxone (10 microM) had also no direct effect on cortisol release. It is concluded that the met-enkephalin analog used in this study and naloxone do affect the hypothalamo-pituitary-adrenal axis via a central effect.
D000236:Adenoma; D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms; D002478:Cells, Cultured; D003366:Cosyntropin / Q000494:pharmacology; D003480:Cushing Syndrome; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology*
D006728:Hormones; D003366:Cosyntropin; D009270:Naloxone; D004744:Enkephalin, Methionine; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin; D013007:Growth Hormone-Releasing Hormone; D006854:Hydrocortisone