Application of a backscattered electron image to immunocytochemistry in freeze-cracked tissues.
A backscattered electron (BSE) image was applied to the observation of freeze-cracked tissue blocks stained with the indirect immunoperoxidase method. On the cracked surface of the pancreas, the BSE image clearly revealed the immunostained B cell granules as bright spots. A pair of secondary electron and BSE images on a fractured surface are useful for the three-dimensional localization of antigens in cells.
D000818:Animals; D005614:Freeze Fracturing; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D012542:Scattering, Radiation
D007328:Insulin