Pepsin can be used to subculture viable mammary epithelial cells.
Normal mouse mammary epithelial cells in primary culture can be passaged as viable single cells using 0.5 to 1.0 mg/ml pepsin in Hanks' salt solution. After 5 min the pepsin treatment preferentially removes fibroblasts, leaving a monolayer of purified epithelial cells that can be removed by pipetting and transferred to new culture vessels or injected into animals.
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004848:Epithelium / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D008321:Mammary Glands, Animal / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010434:Pepsin A; D011247:Pregnancy
D010434:Pepsin A