Mutagenicity evaluation of glutaraldehyde in a battery of in vitro bacterial and mammalian test systems.
Glutaraldehyde was evaluated for genotoxicity using a battery of four in vitro test systems: the Salmonella/microsome assay, the Chinese hamster ovary cell/hypoxanthine-guanine phosphoribosyltransferase (CHO/HGPRT) gene mutation system, the sister-chromatid exchange test with Chinese hamster ovary cells, and measurements of unscheduled DNA synthesis in primary rat-hepatocyte cultures. No significant, dose-related increases in the various end-points were produced by glutaraldehyde in tests with or without the addition of a rat-liver metabolic activation system (S-9 mix) or with the cell-mediated activation of the hepatocyte test system. A range of concentrations which spanned cytotoxic to non-cytotoxic doses was evaluated in each test system and marked cytotoxicity was typically noted at micromolar concentrations. Within a range of biologically active concentrations, glutaraldehyde did not produce significant genotoxic effects with the assays and conditions used for these studies.
D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005796:Genes / Q000187:drug effects; D005976:Glutaral / Q000633:toxicity*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D010053:Ovary; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D000447:Aldehydes; D009153:Mutagens; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase; D005976:Glutaral