[Substrate specificity of phenoloxidase (EC 1.14.18.1)].
Using 10 microns thick cryostat-slides of spleen and gut from white rats the following substances (in 2 different concentrations) were tested as substrates for the enzyme phenoloxidase compared with dihydroxyphenylalanine: pyrogallic acid, resorcin, 2.4-dinitrophenol, tyraminhydrochloride, o-cresol, and epinephrine. Only pyrogallic acid and resorcin resulted in positive reaction. Resorcin showed a positive reaction only when the higher concentration is employed. The investigations show clearly that the detectable enzyme in gut, spleen, and oral cavity is a polyphenoloxidase, which occurs consequently not only in granulocytes.
D000818:Animals; D004156:Catechol Oxidase / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D007422:Intestines / Q000201:enzymology; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011748:Pyrogallol / Q000378:metabolism; D051381:Rats; D012118:Resorcinols / Q000378:metabolism; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity
D012118:Resorcinols; D011748:Pyrogallol; D004295:Dihydroxyphenylalanine; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; C031389:resorcinol
Zur Substratspezifität der Phenoloxidase (EC 1.14.18.1).