A blood group Sda-active pentasaccharide isolated from Tamm-Horsfall urinary glycoprotein.
Human Tamm-Horsfall urinary glycoprotein from an individual of the blood group Sd(a+) phenotype was tritium-labelled by treatment with galactose oxidase and sodium boro[3H]hydride and was then digested with endo-beta-galactosidase. A series of dialysable, labelled fragments was released from which a pentasaccharide was isolated that strongly inhibited the agglutination of Sd(a+) red cells by human anti-Sda serum and hence contained the Sda determinant structure. Reduction, methylation analysis and sequential exo-glycosidase digestion established the structure of the pentasaccharide as: GalNAc beta(1 leads to 4)[NeuAc(2 leads to 3)]Gal beta(1 leads to 4)GlcNAc beta(1 leads to 3)Gal
D001789:Blood Group Antigens / Q000032:analysis*; D002240:Carbohydrate Sequence; D006026:Glycoside Hydrolases; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D009088:Mucoproteins / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis; D058949:Uromodulin; D001616:beta-Galactosidase / Q000378:metabolism
D001789:Blood Group Antigens; D009088:Mucoproteins; D009844:Oligosaccharides; C548612:UMOD protein, human; D058949:Uromodulin; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase