Competitive enzyme immunoassay for apolipoprotein A-II.
A competitive enzyme immunoassay for apolipoprotein A-II was developed. Microtitre plates were used as a solid phase and coated with anti-apolipoprotein A-II antibodies. Purified apolipoprotein A-II, labelled with horseradish peroxidase was used as competing ligand. The assay was examined with respect to the optimal amounts of specific anti-apolipoprotein A-II antibodies and apolipoprotein A-II-enzyme conjugate. The displacement curves showed a good parallelism between serum and purified apolipoprotein A-II. Delipidation of serum did not affect the content of apolipoprotein A-II. Cross-reactivity with apolipoprotein A-I was minimal. The intra- and inter-assay coefficients of variation in the medium range were 9.0 and 11.8% respectively. The assay might be well-suited for clinical routine.
D000906:Antibodies; D000918:Antibody Specificity; D016633:Apolipoprotein A-II; D001053:Apolipoproteins / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D008075:Lipoproteins, HDL / Q000097:blood
D000906:Antibodies; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D007074:Immunoglobulin G; D008075:Lipoproteins, HDL