The quantification of the haemagglutinin content of influenza whole virus and Tween-ether split vaccines.
Monovalent whole virus and Tween-ether split vaccines prepared from influenza A/Bangkok, A/Brazil and B/Singapore were assayed for haemagglutinin content using single radial immunodiffusion (SRID), quantitative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunization of guinea pigs. When SRID was performed with split vaccines, haemagglutinin values were consistently recorded which were in the range of 50 to 25% of the values obtained before disruption of virions. When, however, disruption was conducted in the presence of excess detergent, thus preventing aggregate formation of solubilized haemagglutinin, test values comparable with those of whole virus vaccines were obtained. In agreement with these results, immunization experiments revealed that whole virus and corresponding split vaccines exhibited comparable immunogenicity in guinea pigs. Additionally it could be calculated from SDS-PAGE and densitometer tracings, obtained by scanning the gels after staining with either Coomassie blue or FITC-Con A, that 90 to 95% of whole virus HA2 was recovered in Tween-ether split vaccines. On the basis of these findings we conclude that precise quantification of Tween-ether split vaccines is not possible by the SRID test alone. As aggregate formation of solubilized haemagglutinin occurs, we suggest that either a physico-chemical method including a disaggregation procedure, such as SDS treatment, or immunological evaluation of the original whole virus preparation before disruption of virions should be applied as an additional criterion for quantification of influenza Tween-ether split vaccines.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004987:Ethers; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000032:analysis*; D005779:Immunodiffusion; D007252:Influenza Vaccines / Q000276:immunology*; D009975:Orthomyxoviridae / Q000187:drug effects; D011136:Polysorbates; D011506:Proteins / Q000032:analysis
D004987:Ethers; D006388:Hemagglutinins; D007252:Influenza Vaccines; D011136:Polysorbates; D011506:Proteins