Advantages of preelectrophoretic conjugation of polypeptides with fluorescent dyes.
A rapid simple procedure is described for the conjugation of proteins, glycoproteins, and peptides with the fluorescent dye fluorescein isothiocyanate during the time required to polymerize a polyacrylamide gel. Such conjugation does not perturb the electrophoretic mobility of the polypeptides in detergent containing gels. The location of polypeptide . dye conjugate is evident by inspection immediately upon removal of a gel from an electrophoresis cabinet avoiding the time required for postelectrophoretic staining and destaining procedures. The sensitivity of detection of polypeptide . fluorescein conjugates is at least equivalent to that obtained using Coomassie blue.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006023:Glycoproteins / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D012394:Rosaniline Dyes; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D006023:Glycoproteins; D010455:Peptides; D012394:Rosaniline Dyes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; C004692:coomassie Brilliant Blue