[Determination of the biochemical oxygen demand in nutrient media using microbial flow-through electrodes].
Biochemical oxygen demand (BOD) in some culture media was determined by means of flow-through microbial electrodes, based on Hansenula anomala, Escherichia coli K-12, and activated sludge obtained from the All-Union Scientific-Research Institute of Applied Enzymology (Vilnius). The sensitivity of the electrodes was 112.1, 65.5 and 32.8% O2, respectively, in the presence of 1 mM glucose as substrate. A maximum sensitivity of the electrodes, based on E. coli and activated sludge, was observed on pyruvate oxidation--120 and 82.3% O2 in the presence of 1 mM pyruvate. The yeast electrode exhibited a minimum sensitivity for sucrose--9.3% O2/mM. The time of the electrode response varies from 8 to 35 min, depending on the substrate used. BOD depends on pH of the solution. The microbial electrodes retained the initial sensitivity for 33--38 days. The were used for estimation of BOD in the Rider's and Shopfer's media.
D003470:Culture Media / Q000378:metabolism*; D004566:Electrodes; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism; D012722:Sewage; D012988:Soil Microbiology
D003470:Culture Media; D012722:Sewage; D010100:Oxygen
Opredelenie biokhimicheskoĭ potrebnosti v kislorode v nekotorykh pitatel'nykh sredakh s pomoshch'iu protochnykh mikrobnykh élektrodov.