Immunoglobulin heavy-chain class switching in a pre-B cell line is accompanied by DNA rearrangement.
Switching of an Abelson virus-transformed mouse lymphoid cell line from immunoglobulin mu to gamma 2b heavy-chain synthesis in vitro is accompanied by loss of DNA sequences between the JH and C gamma 2b gene segments, and thus cannot be explained by differential RNA processing. The light-chain loci of both mu- and gamma 2b-producing cell clones are in the embryonic configuration, which indicates that class switching can occur in pre-B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D005786:Gene Expression Regulation; D005796:Genes; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007142:Immunoglobulin gamma-Chains / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D051379:Mice
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains