[Encapsulation of GnRH in liposomes: effects on the release of hypophyseal LH].
The GnRH has been entrapped in liposomes. The infusion of this trapped GnRH in a pituitary superfusion system allows us to show that it mimics only the phase II of the GnRH stimulated LH release. We show that cycloheximide seems to interact at the level of the natural GnRH penetration mechanism in the cytoplasm. One of the places where calcium is suspected to be potent has been located at the expression level of the message transmitted to the cytoplasm by the trapped GnRH.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008081:Liposomes; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D010713:Phosphatidylcholines; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D008081:Liposomes; D010713:Phosphatidylcholines; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D003513:Cycloheximide; D002118:Calcium
Encapsulation du GnRH dans les liposomes: effets sur la libération de la LH hypophysaire.