Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.
Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.
D000818:Animals; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D008647:Mesocricetus; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic
D010455:Peptides; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases