Ammonium sulphate precipitation overestimates titres of anti-peptide antisera.
Precipitation by 50% final saturation of ammonium sulphate, a method commonly used to estimate titres and affinity constants of antibodies, was found consistently to overestimate the titre of anti-peptide antisera when compared with other antibody precipitants. The effect could not be attributed to non-IgG fractions, and similar overestimations were found when pure IgG was used. We suggest that the lower values represent a more physiologically relevant estimation.
D000645:Ammonium Sulfate; D000906:Antibodies / Q000032:analysis*; D011232:Chemical Precipitation; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007158:Immunologic Techniques; D010455:Peptides / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D010455:Peptides; D000645:Ammonium Sulfate