Induction of human gamma interferons by a mitogen derived form Mycoplasma arthritidis and by Phytohemagglutinin: differential inhibition with monoclonal anti-HLA.DR antibodies.
The peripheral blood lymphocytes from eight healthy volunteers produced significant amounts of interferon (IFN) when co-cultured with a recently discovered mitogen preparation derived from cultures of Mycoplasma arthritidis (MAS). Maximum levels of 281 to 2187 U were reached after 3 to 4 days of incubation. The IFN was characterized as IFN gamma on the basis of pH and heat lability, and neutralization with anti-human IFN gamma but not anti-IFN alpha. A monoclonal antibody to HLA.DR determinants suppressed IFN induction and lymphocyte proliferation when co-cultured with MAS but was not effective against PHA-induced IFN and proliferation; PHA-induced proliferation was enhanced in the presence of the antibody. Anti-HLA.DR-mediated inhibition of lymphocyte functions, however, was not specific for MAS inasmuch as viral-induced IFN was also suppressed, as was Con A-induced proliferation. These and other studies suggest that the cellular requirement for T cell activation by MAS, PHA, and Con A may all be distinct.
D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D003208:Concanavalin A / Q000494:pharmacology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology*; D009174:Mycoplasma / Q000032:analysis / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007369:Interferon Inducers; D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A; D007371:Interferon-gamma