[Double labeling of antibodies with fluorescein isothiocyanate (FITC) and horseradish peroxidase (HPOD)].
This paper presents a method for labeling antibodies with fluorescein isothiocyanate (FITC) and additional with horseradish peroxidase (HPOD). With this double labelled antisera the fluorescence-serologic antibody technique (FAT) as well as the enzyme-serologic antibody technique (EAT) was done bit by bit for the same object. How the presented pictures demonstrate the result of both techniques, FAT and EAT, are the same with the described method. So, it is possible after fluorescence-microscopical exploitation through a following histochemical proof of the HPOD to get permanent preparations with the same biological result.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D029822:Enterovirus B, Human / Q000276:immunology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D051379:Mice; D008856:Microscopy, Fluorescence; D013861:Thiocyanates
D000914:Antibodies, Viral; D000956:Antigens, Viral; D005452:Fluoresceins; D013861:Thiocyanates; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate
Doppelmarkierung von Antikörpern mit Fluoreszeinisothiocyanat (FITC) und Meerrettichperoxidase (HPOD).