Fermenter growth of Streptococcus agalactiae and large-scale production of CAMP factor.
Streptococcus agalactiae (group B) was grown in Todd-Hewitt broth (36.4 g l-1, pH 7.8) in a Braun Fermenter (type B20) to investigate the conditions of optimal bacterial growth and maximal production of CAMP factor. The influence of different gas atmospheres (air, N2, CO2, and gas mixtures) on growth, CAMP production and chain length of S. agalactiae was studied. The organisms grew best in the presence of 2% (w/v) glucose, at pH 6.2, with a constant flow of CO2. The number of diplococci and monococci under these conditions reached almost 80% of the total population.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006460:Hemolysin Proteins; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D013292:Streptococcus agalactiae / Q000254:growth & development* / Q000378:metabolism
D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D003470:Culture Media; D006460:Hemolysin Proteins; D002245:Carbon Dioxide; D006859:Hydrogen; D005947:Glucose; D009584:Nitrogen; D010100:Oxygen