Functional testing and chemical composition of cryptococcal extracts.
Three antigens were compared for their ability to detect immune responses in C57Bl/6 mice sensitized to Cryptococcus neoformans. Elicitation of responses in vitro was greatest with a urea extract antigen, followed in efficiency by an alkali extract and a soluble capsular polysaccharide preparation. The reactivity paralleled the protein content of the preparation.
D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D002855:Chromatography, Thin Layer; D003454:Cryptococcus / Q000276:immunology*; D003455:Cryptococcus neoformans / Q000276:immunology*; D005656:Fungal Proteins / Q000032:analysis; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D008055:Lipids / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008851:Micropore Filters; D008970:Molecular Weight; D011134:Polysaccharides / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000946:Antigens, Fungal; D002241:Carbohydrates; D005656:Fungal Proteins; D008055:Lipids; D011134:Polysaccharides; C014426:cryptococcal polysaccharide