Stabilities of some constituents of marmoset (Callithrix jacchus) plasma under various conditions of storage.
The stability of various marmoset (Callithrix jacchus) plasma constituents was investigated after storage at room temperature, 4 degrees C, and -20 degrees C. The method of sequential analysis ensured that the between-run bias of the methods of analysis used was drastically reduced, and the definitions of stability were linked to the imprecision of these methods. Optimal conditions for storage for as long as 48 h depended on the analyte being measured. Room temperature was optimal for cholinesterase and acetylcholinesterase; 4 degrees C for protein, albumin, alanine aminotransferase, isocitrate dehydrogenase, sorbitol dehydrogenase, lactate dehydrogenase, and glutamate dehydrogenase; and -20 degrees C for glutathione reductase and alkaline phosphatase. For aspartate amino-transferase and gamma-glutamyltransferase, either 4 degrees C or -20 degrees C would be suitable. Reasons are advanced for some conflicting reports in the published work, and we emphasize the need to investigate each analyte and species separately.
D000818:Animals; D001793:Blood Preservation; D002144:Callithrix / Q000097:blood*; D002143:Callitrichinae / Q000097:blood*; D003080:Cold Temperature; D004798:Enzymes / Q000097:blood* / Q000592:standards; D013048:Specimen Handling; D013997:Time Factors; D014116:Toxicology
D004798:Enzymes