Chromatographic separation of coelomic fluid from Holothuria polii (echinodermata) and partial characterization of the fractions reacting with erythrocytes.
Coelomic fluid preparations from Holothuria polii were passed through a Bio-gel A5m column. The 3 separated protein peaks possess hemagglutinating or hemolytic activity against rabbit erythrocytes. Electrophoretic and immunochemical methods showed that 2 identical protein subunits characterized hemagglutinins of different size. Hemolysin differs from hemagglutinin in molecular weight and organization of subunits.
D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods; D004447:Echinodermata / Q000032:analysis*; D004912:Erythrocytes / Q000187:drug effects*; D006388:Hemagglutinins / Q000302:isolation & purification*; D006460:Hemolysin Proteins / Q000302:isolation & purification*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011817:Rabbits
D006388:Hemagglutinins; D006460:Hemolysin Proteins