Simple screening of Bence-Jones proteins from unconcentrated urine.
A rapid, sensitive and simple method is provided for detecting Bence-Jones proteins in unconcentrated urine by electrosyneresis. This is based on the fact that the free light-chains, similar to the intact immunoglobulin molecules, form complexes with albumin under the effect of glutaraldehyde. Consequently, the originally cathode-oriented Bence-Jones proteins will also migrate toward the anode against the corresponding specific immunoserum. At a 200 mg/ml excretion rate of the free light-chain (corresponding to 0.3 mg/24 h Bence-Jones protein in 1500 ml daily urine) the reaction is still readily assessed. The method presented here meets the requirements of a routine clinical laboratory.
D000937:Antigen-Antibody Reactions; D001536:Bence Jones Protein / Q000652:urine*; D003378:Counterimmunoelectrophoresis; D007147:Immunoglobulin Light Chains / Q000652:urine; D009101:Multiple Myeloma / Q000175:diagnosis; D011507:Proteinuria / Q000175:diagnosis
D007147:Immunoglobulin Light Chains; D001536:Bence Jones Protein