Serine: glyoxylate, alanine:glyoxylate, and glutamate:glyoxylate aminotransferase reactions in peroxisomes from spinach leaves.
Two different aminotransferases, that have glyoxylate as the amino acceptor, have specific activities of 1 to 2 mumol . min-1 . mg of protein-1 in the isolated peroxisomal fraction from spinach leaves. Their properties were evaluated after separation on a hydroxylapatite column. Both enzymes had a Km for glyoxylate of 0.15 mM and an amino acid Km of 2 to 3 mM. Reactions proceeded by a Ping Pong Bi Bi mechanism. Serine:glyoxylate aminotransferase was relatively specific for both substrates and could only be slightly reversed with 100 mM glycine, although the Ki of glycine was 33 mM. The glutamate:glyoxylate amino-transferase protein was equally active in catalyzing an alanine:glyoxylate aminotransferase reaction, but the reverse reactions with 100 mM glycine were hardly measureable, although the Ki (glycine) was 8.7 mM. Protection against hydroxylamine inhibition from reaction with pyridoxal phosphate was used to investigate the specificity of amino acid binding. Substrate amino acids protected at about the same concentration as their Km, while glycine protected at its Ki concentration. Thus, the nearly irreversible catalysis with glycine is not due to a failure to bind glycine. The significance of a peroxisomal alanine:glyoxylate aminotransferase activity has not been incorporated into schemes for the oxidative photosynthetic carbon cycle.
D000410:Alanine Transaminase / Q000302:isolation & purification / Q000378:metabolism*; D006038:Glyoxylates / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008830:Microbodies / Q000201:enzymology*; D009940:Organoids / Q000201:enzymology*; D010944:Plants / Q000201:enzymology*; D012694:Serine / Q000302:isolation & purification / Q000378:metabolism; D000637:Transaminases / Q000302:isolation & purification / Q000378:metabolism*
D006038:Glyoxylates; D012694:Serine; D000637:Transaminases; C020649:glutamate-glyoxylate aminotransferase; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase; C022636:serine-glyoxylate aminotransferase