[Ultrastructural detection of the heterogeneity of glycocalyx in convoluted and straight proximal tubules of rat kidney by the lectin-gold complex technic].
A new histochemical technique in which lectin-gold complexes are applied directly to thin and semi-thin sections of tissue was used to determine the distribution of Helix pomatia lectin binding sites in the proximal tubule of Rat kidney. Whereas a strong labeling was seen on the brush border and baso-lateral plasma membranes of convoluted tubules, the plasma membrane of cells from the medullary straight segment was unlabeled. Some profiles at the frontier between cortex and medulla had a mosaic of positive and negative cells. These results show, therefore, considerable segmental variation in the glycocalyx composition of Rat proximal tubules.
D000818:Animals; D006023:Glycoproteins / Q000032:analysis*; D006046:Gold; D006651:Histocytochemistry; D007687:Kidney Tubules, Proximal / Q000032:analysis / Q000648:ultrastructure*; D037102:Lectins; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011975:Receptors, Mitogen / Q000032:analysis
D006023:Glycoproteins; C038095:Helix lectin; D037102:Lectins; D011134:Polysaccharides; D011975:Receptors, Mitogen; D006046:Gold
Détection ultrastructurale de l'hétérogénéité du glycocalyx dans les tubes contournés et droits proximaux du rein de Rat par la technique de la lectine couplée à l'or [1].