Effect of progesterone alone and in combination with either corticosterone, 17 beta-estradiol or insulin on prolactin-stimulated fatty acid synthesis in mammary explants from pseudopregnant rabbits.
Progesterone up to 15.9 mumol/l progressively suppressed prolactin-stimulated fatty acid synthesis in cultured mammary explants from 11-day pseudopregnant rabbits, but did not influence the proportion of fatty acids of medium chain length (C8:0-C12:0). Greater sensitivity to progesterone inhibition was observed at the low end of the ranges of corticosterone (29 nmol/l) and insulin (4.2 nmol/l) concentrations in the culture medium, which suggests an interaction between the hormones. A range of from 0.0001 to 10 mumol/l of 17 beta-estradiol concentrations had no effect on prolactin-stimulated fatty acid synthesis in the presence or absence of progesterone.
D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D046508:Culture Techniques; D004958:Estradiol / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008321:Mammary Glands, Animal / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D011388:Prolactin / Q000494:pharmacology*; D011555:Pseudopregnancy / Q000378:metabolism*; D011817:Rabbits
D005227:Fatty Acids; D007328:Insulin; D007773:Lactates; D019344:Lactic Acid; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D003345:Corticosterone