Ethanol-soluble substances isolated from Escherichia coli culture filtrate induce polymorphonuclear chemotaxis.
Substances chemotactic (CT) for human PMN were isolated from the culture medium of Escherichia coli O2 : Kl grown to stationary phase. The ethanol soluble fraction of the lyophilized culture filtrate, which contained the bulk of CT activity, was gel filtrated on Sephadex G-10. The 5 X 10(3) molecular weight fraction showed the highest CT activity. On the basis of partial chemical characterization the active CT substances probably consist of glycopeptides and lipids originating from the cell envelope.
D000596:Amino Acids / Q000032:analysis; D002630:Chemotactic Factors / Q000032:analysis / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000502:physiology*; D000431:Ethanol; D006020:Glycopeptides / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis; D009504:Neutrophils / Q000502:physiology; D012995:Solubility
D000596:Amino Acids; D002630:Chemotactic Factors; D006020:Glycopeptides; D008055:Lipids; D000431:Ethanol