Demonstration of light chain monotypia in B cell non-Hodgkin's lymphomas using unfixed freeze-dried and formalin-fixed trypsinised paraffin sections.
Immunohistological light chain analysis was carried out in 55 patients with non-Hodgkin's lymphoma of B cell origin. Unfixed freeze-dried paraffin sections were used to detect surface Ig and formaldehyde-fixed trypsinised sections to detect cytoplasmic Ig. Cytoplasmic Ig was seen inconstantly in freeze-dried paraffin sections. There was complete agreement with regard to the type of light chain between freeze-dried and formaldehyde-fixed trypsinised sections. Immunohistology showed the monotypic immunoglobulin light chains in 83% of cases when unfixed freeze-dried paraffin sections were used for sIg demonstration while only in 45% of cases when formaldehyde-fixed material was used for cIg detection. The efficiency of sIg demonstration in unfixed freeze-dried paraffin sections was comparable with that based on unfixed cryostat sections or on cell suspensions when the lymphocytic lymphoma/CLL group was excluded from the evaluation. The relatively low frequency of monotypic sIg positivity obtained in this group (12/19) is due to the low density of surface Ig in CLL lymphocytes.
D001402:B-Lymphocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D005612:Freeze Drying; D006801:Humans; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007943:Leukemia, Hairy Cell / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D007147:Immunoglobulin Light Chains; D011947:Receptors, Antigen, B-Cell; D005557:Formaldehyde