Isolation and characterization of 15-hydroxylated metabolites of leukotriene C4.
A polar metabolite of leukotriene C4 was formed by sequential conversions with soybean lipoxygenase I and liver peroxidase. The structure of this product was found to be 5(S), 15(S)-dihydroxy-6(R)-S-glutathionyl-7,9,13-trans-11-cis-eicosatetraenoic acid (15-hydroxy-delta 13-trans-leukotriene C3. The HPLC behaviour, the molar extinction coefficient and the biological activity of the metabolite are reported. Preliminary evidence suggests that this product is formed by mammalian tissues.
D000818:Animals; D001095:Arachidonic Acids; D002851:Chromatography, High Pressure Liquid; D015777:Eicosanoids; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D007700:Kinetics; D008084:Lipoxygenase / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D009842:Oligopeptides; D010944:Plants / Q000201:enzymology; D013189:SRS-A / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013025:Glycine max
D001095:Arachidonic Acids; D015777:Eicosanoids; D009842:Oligopeptides; D013189:SRS-A; C073550:lipoxin C4; D008084:Lipoxygenase