Enzyme-immunoassay: a review.
The main principles of heterogeneous and homogeneous enzyme-immunoassays are reviewed. Furthermore, an overview is given of enzymes currently used as labels and methods applied for conjugate preparation. The reliability and practicability of enzyme-immunoassays are discussed. Special consideration is given to possible interferences, the detection limits and the mechanization of these assays. A range of curve-fitting methods for evaluation of the results is listed. Future prospects for the further development of enzyme-immunoassay and application of this technique are discussed.
D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D010783:Photometry; D013972:Thyrotropin / Q000032:analysis
D004798:Enzymes; D013972:Thyrotropin