Measurement of parasite-specific antibody responses using a tritiated avidin-solid phase radioimmunoassay.
A solid phase radioimmunoassay (RIA) to measure parasite-specific antibody responses has been developed. The assay utilizes 3H-labeled avidin as the indicator molecule and represents a marked improvement in detection of specific antibodies to Trypanosoma cruzi over previously used RIA. Avidin is easily labeled to high efficiency (3.6 X 10(5) cpm/micrograms) under very mild conditions using N-succinimidyl-[2,3-3H]propionate, and the high efficiency of avidin-biotin binding coupled with the increased safety and longer half-life of the labeled reagent give this method advantage over currently used 125I-labeled Ab RIAs.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001360:Avidin / Q000378:metabolism*; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000031:analogs & derivatives*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D014349:Trypanosoma cruzi / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D001360:Avidin; D010047:Ovalbumin