Immunonephelometric determination of retinol-binding protein in serum and urine.
An immunonephelometric method developed for measurement of retinol-binding protein (RBP) in serum and urine can detect it in concentrations of about 30 micrograms/L, which is in the lower limit of its normal concentration in urine (range 0-0.56 mg/L; mean +/- SD 0.19 +/- 0.15; n = 44). Urinary RBP was increased (range 0.93-29.5 mg/L) in all of 25 urine specimens from 13 subjects being treated with aminoglycoside (tobramycin). Urinary excretion of RBP was correlated (r = 0.83) with the excretion of beta 2-microglobulin. The within-assay and day-to-day precision (CV) was determined over the detection range of 0.03-8 mg/L. Within these limits the corresponding CVs varied from 4 to 27% and from 8 to 30%, respectively. The method had fairly good precision within the optimal measuring range of approximately 0.4 to 4.5 mg/L for both urine and 20-fold diluted serum samples. For various RBP concentrations our analytical recovery was 89-114% of added RBP. Results by this method correlated well (r = 0.96, n = 24) with those by a radial immunodiffusion method.
D005779:Immunodiffusion; D007674:Kidney Diseases / Q000139:chemically induced / Q000652:urine; D007834:Lasers; D008099:Liver / Q000378:metabolism*; D009391:Nephelometry and Turbidimetry; D012177:Retinol-Binding Proteins / Q000032:analysis* / Q000097:blood / Q000652:urine; D014031:Tobramycin / Q000009:adverse effects; D014801:Vitamin A / Q000097:blood; D001613:beta 2-Microglobulin / Q000652:urine
D012177:Retinol-Binding Proteins; D001613:beta 2-Microglobulin; D014801:Vitamin A; D014031:Tobramycin