Cleaving of disulfide bridges and apparent molecular weight of human prolactin variants as revealed by immunoperoxidase electrophoresis.
Five highly purified preparations of human pituitary prolactin, widely used in radioimmunoassays, were analyzed by immunoperoxidase electrophoresis with rabbit antisera raised against ovine and rat prolactin. When unreduced, the prolactin content of these preparations was separated into one major prolactin-like immunoreactive band of Mr 23,000, and four fainter immunoreactive bands of Mr's 16,000, 22,000, 25,000, and 45,000. Increasing the 2-mercaptoethanol concentration modified the relative proportions and the Mr of immunoreactive bands so that, after extensive reduction, only two immunoreactive bands were separated, one major band of Mr 25,000 and one fainter band of Mr 17,000. A direct relationship existed between the load of prolactin submitted to electrophoresis in the 0.25 to 75 ng range and the optical density of the separated bands.
D000818:Animals; D001952:Bridged-Ring Compounds; D004220:Disulfides; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010902:Pituitary Gland; D011388:Prolactin / Q000302:isolation & purification*; D051381:Rats; D012756:Sheep
D001952:Bridged-Ring Compounds; D004220:Disulfides; D011388:Prolactin