[Combined effect of photoreactivation and temperature on the survival of cells from E. coli B, irradiated with short-wave UV light].
A study was made of the survival curves of E. coli B(fil+/lon-) exposed to far UV-light (lambda = 254 nm) plated immediately after liquid holding recovery and after photoreactivation and incubated overnight at 37 degrees and 42 degrees. It was shown that the survival rate was always higher at 42 degrees C irrespective of the modification technique applied. Since the temperature-induced modification was significant after complete elimination of pyrimidine dimers (PD) a conclusion was made that UV-light, in addition to PD, induced just one more type of damages modified by temperature.
D004260:DNA Repair / Q000528:radiation effects; D004269:DNA, Bacterial / Q000528:radiation effects; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000528:radiation effects*; D011829:Radiation Dosage; D011836:Radiation Tolerance; D013696:Temperature; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase
Kombinirovannoe deĭstvie fotoreaktivatsii i temperatury na vyzhivaemost' kletok E. coli B, obluchennykh korotkovolnovym UF-svetom.