Automated measurement of acid phosphatase reaction product in human kidneys treated with aminoglycoside antibiotics.
The acid phosphatase reaction product was measured by automated image analysis densitometry in histologic sections from 23 human kidney biopsies. Optical density was proportional to incubation time, section thickness and enzyme activity. After a four days' treatment with aminoglycoside antibiotics, optical density was increased and paralleled the lysosomal changes previously observed by electron microscopy.
D000135:Acid Phosphatase / Q000378:metabolism*; D000617:Aminoglycosides / Q000627:therapeutic use*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003720:Densitometry / Q000379:methods; D006652:Histological Techniques; D007668:Kidney / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure; D008247:Lysosomes / Q000648:ultrastructure; D013997:Time Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000135:Acid Phosphatase