Respiratory chain of bacterial membrane: assembly-mobile carriers equilibrium.
The target size of NADH-oxidase activity of M. lysodeikticus isolated membranes for electron radiation is nearly equal to that obtained for NADH-dehydrogenase (about 50 kD). The complete cross-linking of membrane proteins by glutaraldehyde causes an increase of NADH-oxidase target size to 3-3.5 times its original value. Electrons are transported by cross-linked respiratory chain from NADH to O2 with 60-50% effectiveness of that in untreated membranes. It is proposed that electrons are transported through a multi-enzymic complex of individual carriers having limited lifetime with exchange of carriers between different respiratory complexes via lateral diffusion in membrane.
D002462:Cell Membrane / Q000502:physiology; D004579:Electron Transport / Q000528:radiation effects; D005976:Glutaral; D008560:Membrane Fluidity; D008837:Micrococcus / Q000502:physiology*; D009097:Multienzyme Complexes / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism
D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D009245:NADH Dehydrogenase; D005976:Glutaral