Expression of T-lymphoblast-encoded HLA-DR, MT, and SB antigens on human T-B lymphoblast hybrids.
The Ia-like antigens of hybrids of the B-lymphoblastoid cell line (B-LCL) WI-L2 and a subline of the T-lymphoblastoid cell line (T-LCL) HSB were examined. Antigens of the HLA-DR and MT series were analyzed by indirect immunofluorescence with chimpanzee anti-DR sera and monoclonal antibodies, and antigens of the SB series were analyzed by primed lymphocyte typing (PLT). The WI-L2 X HSB hybrids expressed antigens of each series not found on either parent cell. In each case, the novel antigens were indistinguishable from those found on SB, a B-LCL established from the same individual as HSB, and are thus presumably HSB encoded.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D005802:Genes, MHC Class II; D005815:Genetic Code; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D002679:Pan troglodytes; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II