Ultrastructural differentiation within the central nervous system between indolamines and catecholamines by energy dispersive X-ray analysis following paraformaldehyde pretreatment.
Dense bodies containing high amounts of chrome were localized in the perikarya of substantia nigra and dorsal raphe neurons following the cytochemical reaction of endogenous dopamine and serotonin (respectively) with glutaraldehyde-dichromate (GDC). Energy dispersive X-ray analysis of these bodies revealed chrome levels two to four times higher than those recorded from the cytoplasmic background. Pretreatment with paraformaldehyde blocked the GDC reaction within the dense bodies in the substantia nigra (chrome levels similar to background), while the chrome levels in the dense bodies of the raphe neurons remained elevated. This demonstrates that pretreatment with paraformaldehyde allows selective localization of central nervous system serotonin stores by the GDC technique.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002840:Chromates; D004577:Electron Probe Microanalysis; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D008297:Male; D009474:Neurons / Q000032:analysis; D011108:Polymers; D011903:Raphe Nuclei / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*; D013378:Substantia Nigra / Q000032:analysis
D002395:Catecholamines; D002840:Chromates; D011108:Polymers; D005557:Formaldehyde; D012701:Serotonin; C034944:chromic acid; D005976:Glutaral; C003043:paraform