Molecular events in the processing of avidin by antigen-presenting cells (APC). III. Activation of T-lymphocyte lines and H-2 restriction are mediated by processed avidin associated with I-region gene products.
We investigated the interaction between processed avidin (PA) and avidin-specific lines of T lymphocytes free of resident antigen-presenting cells (APC). We found that PA was able to replace the requirement for APC in the T-lymphocyte proliferative assay, only if the PA was associated with an Ia-positive moiety (IPM) supplied by the APC. In addition to supplying a necessary signal for a proliferative response to PA, IPM imposed H-2 restriction on the PA molecule. The association between PA and IPM was reversible and the two moieties could be physically separated and recombined. The results support a conclusion that major histocompatibility restriction of the interaction between T lymphocytes and APC is due to the association between processed antigen and an APC element containing I-region products.
D000818:Animals; D001360:Avidin / Q000276:immunology*; D002478:Cells, Cultured; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D051379:Mice; D010047:Ovalbumin / Q000031:analogs & derivatives*; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D001360:Avidin; D010047:Ovalbumin