Diversity and joining segments of mouse immunoglobulin heavy chain genes are closely linked and in the same orientation: implications for the joining mechanism.
We have mapped 12 diversity (D) gene segments of mouse Ig heavy chains between 80 and 1 kilobases 5' to the four joining (JH) gene segments by molecular analysis of DNA clones isolated from recombinant phage and cosmid libraries. All identified D and JH segments are in the same 5'-3' orientation. The significance of these findings with respect to the joining mechanism for Ig gene segments is discussed.
D000818:Animals; D000916:Antibody Diversity; D001483:Base Sequence; D002874:Chromosome Mapping; D008040:Genetic Linkage; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic
D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains