Comparison of antibody elution techniques by enzyme-linked antiglobulin test.
The enzyme-linked antiglobulin test was used to determine the percentage of antibody removed from sensitized red cells by five elution methods: Rubin ether, xylene, digitonin-acid, glycine, and heat. Antibodies examined in the study included anti-D, -c, -E, and -K. With two examples of anti-D, more antibody was eluted by the Rubin ether method (45.5% average) than the xylene (38%) or the digitonin acid method (35%) (p less than 0.05); the glycine (8%) and heat method (15%) were less efficient. With one example of anti-E and one example of anti-c, more antibody was eluted by the ether method. The percentage of anti-K recovery, however, was greater with the digitonin acid method (45%) than with the ether (30%). We found the enzyme-linked antiglobulin test method useful in the quantitative evaluation of elution procedures.
D000906:Antibodies / Q000302:isolation & purification*; D003298:Coombs Test; D004072:Digitonin; D004987:Ethers; D005998:Glycine; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques; D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System; D012710:Serum Albumin, Bovine
D000906:Antibodies; D004987:Ethers; D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System; D012710:Serum Albumin, Bovine; D004072:Digitonin; D005998:Glycine