Lectins in the unicellular Tetrahymena. I. Lectin detection with FITC-labeled anti-lectins.
FITC-labeled antibodies prepared in rabbits to bean, pea, lens, datura, and snail lectin indicated by immunological reaction presence in the Tetrahymena of corresponding lectin-like components. The latter localized partly on the surface, partly inside the body of the unicellular. The specificity of the anti-lectins was ascertained by immunological and hemagglutination tests, and by examining antibody binding after pretreatment of the Tetrahymena with lectin or sugar.
D000818:Animals; D000918:Antibody Specificity; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006386:Hemagglutination Tests; D037102:Lectins / Q000032:analysis* / Q000276:immunology; D037121:Plant Lectins; D010944:Plants; D013768:Tetrahymena / Q000032:analysis*; D013861:Thiocyanates
D005452:Fluoresceins; D037102:Lectins; D037121:Plant Lectins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate