Release of immunoreactive TRH in serum-free cultures of mouse hypothalamic cells.
Serum-free cultures of mouse hypothalamic cells were used as a model for studying TRH (thyroliberin) secretion in vitro. Supplementation of the culture medium with triiodothyronine, corticosterone and polyunsaturated fatty acids is necessary to obtain a substantial release capacity of TRH neurons. Under these conditions depolarization of the cells with 60 mM K+ results in a calcium-dependent release of immunoreactive TRH.
D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology; D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005333:Fetus; D007031:Hypothalamus / Q000378:metabolism*; D051379:Mice; D008959:Models, Neurological; D011188:Potassium / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology
D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D014284:Triiodothyronine; D013973:Thyrotropin-Releasing Hormone; D011188:Potassium; D003345:Corticosterone