Rates of catabolism calculated from 14CO2 production: artifacts and realities.
We present evidence that the metabolism of acetate and ethanol by the liver markedly decreases the yield of label from mitochondrial [14C]acetyl-CoA to 14CO2. The production of [14C]acetyl-CoA from a [14C]labeled substrate can be calculated from the production of 14CO2 if one assesses (1) 14CO2 reincorporation and (2) the yield of label from [14C]acetyl-CoA to CO2.
D000085:Acetates / Q000378:metabolism; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002952:Citric Acid Cycle; D000431:Ethanol / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D002245:Carbon Dioxide; D000431:Ethanol; D000105:Acetyl Coenzyme A