Electrofusion of myeloma cells on the single cell level. Fusion under sterile conditions without proteolytic enzyme treatment.
A technique is presented which allows electrofusion of single cells under sterile conditions. The electrofusion chamber is placed in a Petri dish. Before a droplet of the fusion medium is pipetted between the electrodes, the chamber is completely covered with vaseline, which prevents the fusion medium evaporating. Additionally, the fusion chamber is treated with solutions containing poly(L)-lysine and pronase which results in a decreased movement of the cells on the glass between the electrodes and which allows electrofusion without any proteolytic pretreatment.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D004566:Electrodes; D006825:Hybridomas / Q000503:physiopathology; D008239:Lysine; D051379:Mice; D010954:Plasmacytoma / Q000503:physiopathology*; D011402:Pronase
D011402:Pronase; D008239:Lysine