Demonstration of two unique metabolites of arachidonic acid from phorbol ester-stimulated bovine lymphocytes.
Treatment of bovine lymphocytes with phorbol esters activates an oxygen-dependent system for the formation of a class of arachidonic acid metabolites which appears distinct from prostaglandins, prostacyclins, thromboxanes and leukotrienes. A full activation of the system is achieved within minutes by 10(-7) M 12-O-tetradecanoylphorbol-13-acetate. This activation can be largely prevented by the lipoxygenase inhibitors, 5,8,11,14-eicosatetraynoic acid and nordihydroguaiaretic acid.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002396:Catechols / Q000494:pharmacology; D002417:Cattle; D016861:Cyclooxygenase Inhibitors; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism*; D009637:Masoprocol; D010703:Phorbol Esters / Q000494:pharmacology; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D001095:Arachidonic Acids; D002396:Catechols; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010703:Phorbol Esters; D010704:Phorbols; D015117:5,8,11,14-Eicosatetraynoic Acid; D016718:Arachidonic Acid; D009637:Masoprocol; D008084:Lipoxygenase; D013755:Tetradecanoylphorbol Acetate