[In vitro study of the lipase activity of bacterial strains isolated from the rabbit cecum on C14-labeled triolein].
The lipase activity of bacterial species isolated from the caecum of conventional rabbits was studied by means of two groups of tests: (1) bacteriologic isolation and an identification of nine aerobic and anaerobic clones and (2) incubation of each of these clones in the appropriate medium in the presence of labelled triolein (oleyl 1-14C), a biochemical analysis of the radioactivity of the fractions obtained. The main results can be expressed in the following way: maximum hydrolysis of glycerides is achieved by two species: Bacillus licheniformis and Clostridium sporogenes. The reaction is proportional to the incubation time for incubations of less than 8 h. The release of fatty acids is concomitant to the synthesis of phospholipids and of fatty acid methyl esters.
D000818:Animals; D001407:Bacillus / Q000201:enzymology; D001409:Bacillus cereus / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D002432:Cecum / Q000382:microbiology; D003013:Clostridium / Q000201:enzymology; D013293:Enterococcus faecalis / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D008049:Lipase / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000096:biosynthesis; D011817:Rabbits; D013291:Streptococcus / Q000201:enzymology; D014304:Triolein / Q000378:metabolism*
D010743:Phospholipids; D014304:Triolein; D008049:Lipase
Etude in vitro de l'activité lipasique de souches bactériennes isolées de caecum de lapins sur la trioléine marquée au 14C.