Photoaffinity reagents for use with pepsin and other carboxyl proteases.
Two compounds have been designed to serve as photoaffinity reagents for use with carboxyl proteases. 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propane has been synthesized and shown to react with porcine pepsin in the same fashion as the traditional inhibitor 1,2-epoxy-3-(p-nitrophenoxy)propane, while p-azidophenacyl bromide is similar to other phenacyl bromides in its reaction with pepsin. In combination with p-azido-alpha-diazoacetophenone, previously shown to resemble alpha-diazo carbonyl reagents in its reaction with pepsin, photoaffinity analogs are now available for all three of the widely-used carboxyl protease inhibitors.
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases; D001386:Azides / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D010434:Pepsin A / Q000378:metabolism*; D010777:Photochemistry; D013552:Swine
D000345:Affinity Labels; D001386:Azides; C025608:4-azido-alpha-diazoacetophenone; C037656:1,2-epoxy-3-(4'-azido-2'-nitrophenoxy)propane; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; D010434:Pepsin A; C011171:4-azidophenacyl bromide