Monoclonal antibodies to canine cell surface antigens: a comparison of screening assays.
An ELISA for detection of monoclonal antibodies to surface antigens on canine lymphocytes was evaluated, and results were compared with three additional tests: a radioimmunoassay using 125I-labeled staphylococcal protein A (IPA), the fluorescence activated cell sorter (FACS II), and CdL. Three potentially interesting hybridomas were subsequently selected from two fusions.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D013205:Staphylococcal Protein A