Single-tracer technique to evaluate pulmonary edema and its application to detect the effect of hexamethylene diisocyanate trimer aerosol exposures.
Two hours after a four-hour exposure to hexamethylene diisocyanate trimer (HDIt) aerosol between 2.5 and 39 mg/m3, mice were injected iv with 51Cr-EDTA (chromium ethylenediaminetetraacetate). Ten minutes later the lung was lavaged. A larger amount of 51Cr-EDTA was detected in the lung lavage of HDIt mice than of controls in a concentration-related fashion. The concentration-response curve was shifted to the left compared with that constructed using lung weight increase as response. Kinetic studies of the plasma level of 51Cr-EDTA revealed a three-exponential profile in normal mice, and similar plasma levels were obtained with mice exposed to 18-24 mg/m3 HDIt. However, both the amount of 51Cr-EDTA in the alveolar space and concentration in the pulmonary extravascular compartment were higher in HDIt-exposed mice than in controls. The data of 51Cr-EDTA distribution in the lung were fitted with a three-compartment model. According to the model, HDIt exposures increase the permeability constants of 51Cr-EDTA transport into the alveolar space from blood which accounts for the larger amount of 51Cr-EDTA in lung lavage of HDIt-exposed mice. This 51Cr-EDTA injection and lung lavage technique is a sensitive method for detecting pulmonary edema.
D000336:Aerosols; D000818:Animals; D002860:Chromium Radioisotopes; D003485:Cyanates / Q000633:toxicity*; D004195:Disease Models, Animal; D004492:Edetic Acid / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D011650:Pulmonary Alveoli / Q000187:drug effects; D011652:Pulmonary Circulation; D011654:Pulmonary Edema / Q000139:chemically induced*
D000336:Aerosols; D002860:Chromium Radioisotopes; D003485:Cyanates; C038763:hexamethylene diisocyanate trimer; D004492:Edetic Acid