Clustering of mutations affecting central pathway enzymes of carbohydrate catabolism in Pseudomonas aeruginosa.
Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated transduction. Loci for all three genes were located in the 45- to 55-min region of the chromosome; both zwf-1 and edd-1 were linked by transduction to nalA, whereas pyc-2 was linked by conjugation to argF10. The zwf-1 mutation exhibited cotransduction frequencies of greater than 95% with both edd-1 and the hex-9001 marker, a mutation reported to prevent growth on hexoses. The latter mutation was shown to cause a specific deficiency in 2-keto-3-deoxy-6-phosphogluconate aldolase activity and was redesignated eda-9001. These results demonstrate tight clustering of the gene loci for glucose 6-phosphate dehydrogenase and for both enzymes unique to the Entner-Doudoroff pathway in P. aeruginosa. Our evidence suggests supraoperonic clustering of these and other inducible carbohydrate catabolic genes in the 45- to 55-min region of the chromosome.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D008040:Genetic Linkage; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006836:Hydro-Lyases / Q000235:genetics*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D011766:Pyruvate Carboxylase / Q000235:genetics*; D014161:Transduction, Genetic
D005954:Glucosephosphate Dehydrogenase; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase; D011766:Pyruvate Carboxylase