Deletion analysis of the Escherichia coli ara PC and PBAD promoters.
Deletions extending various distances into the ara PC-PBAD regulatory region were studied to define the sites required in vivo for the activity of these promoters. Deletions from the PC side entering the CRP site, which is located from -80 to -120 with respect to the PBAD transcription start site, reduced activity of this promoter. Similarly, deletions entering this site from the PBAD side reduced activity of the PC promoter. Cyclic AMP receptor protein bound at this site apparently functions to stimulate transcription of both flanking promoters.
D051777:AraC Transcription Factor; D001089:Arabinose / Q000235:genetics*; D001426:Bacterial Proteins; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005686:Galactokinase / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009876:Operon; D011953:Receptors, Cyclic AMP / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors; D014158:Transcription, Genetic
D051777:AraC Transcription Factor; C497371:AraC protein, E coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011953:Receptors, Cyclic AMP; D012097:Repressor Proteins; D014157:Transcription Factors; D001089:Arabinose; D005686:Galactokinase; D012321:DNA-Directed RNA Polymerases