Isolation and partial characterization of a cerulenin-sensitive mutant of Pseudomonas aeruginosa.
Sensitivity of Pseudomonas aeruginosa to cerulenin was first tested. The result indicated that this bacterium is resistant to cerulenin. Cerulenin-sensitive mutants were isolated from P. aeruginosa PML 1552 by 1-methyl-3-nitro-1-nitrosoguanidine treatment and following carbenicillin plus D-cycloserine screening. Isolated mutants were designated CSM-1 to CSM-19, and some characters of CSM-19, which showed rapid growth almost as well as parent strain in the medium without cerulenin, were examined. The cell growth of CSM-19 was greatly inhibited by 50 micrograms/ml of cerulenin, but when the mixture of cellular fatty acids or both cis-vaccenic acid and palmitic acid were added to the medium, the growth was partially recovered. Incorporation of radioactivity into fatty acids from [1-14C]acetate was lowered by cerulenin. Those results mean that the fatty acid synthesis of CSM-19 was decreased by cerulenin. Although cellular fatty acid composition and amount were not notably different between CSM-19 and PML 1552, CSM-19 had less phosphatidylethanolamine, and more phosphatidylglycerol and cardiolipin than PML 1552. CSM-19 was also supersensitive to several other antibiotics, especially to carbenicillin and tetracycline, when compared with PML 1552, although both strains showed identical sensitivity to D-cycloserine, polymyxin B, and chloramphenicol.
D000900:Anti-Bacterial Agents / Q000633:toxicity; D000935:Antifungal Agents / Q000633:toxicity*; D002569:Cerulenin / Q000633:toxicity*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010743:Phospholipids / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010743:Phospholipids; D008769:Methylnitronitrosoguanidine; D002569:Cerulenin