Isolation and characterization of a specific enterokinase inhibitor from kidney bean (Phaseolus vulgaris).
A specific enterokinase inhibitor from kidney bean (Phaseolus vulgaris) was purified to homogeneity. It showed a single protein band on sodium dodecyl sulphate/polyacryl-amide-gel electrophoresis in the presence of mercaptoethanol, and the Mr was 31000. Aspartic acid was identified as the N-terminus of the inhibitor. The Mr by gel chromatography on Sephadex G-200 was found to be 60000, indicating the dimeric nature of the inhibitor. The inhibitor was found to be a glycoprotein. The monosaccharide moieties were glucose, mannose, glucuronic acid and glucosamine in the proportions 3.15%, 5.0%, 0.85% and 1.3% respectively. The inhibitor was most active on pig enterokinase, followed by bovine and human enterokinases. Maximal inhibitory activity was elicited by preincubation of the inhibitor with the enzyme for 15 min. Digestion with pepsin resulted in loss of inhibitory activity. The inhibitor was stable to exposure to a wide range of pH values (2-10), and exposure to pH above 10 resulted in loss of inhibitory activity. Modification of arginine residues by cyclohexane 1,2-dione and ninhydrin led to complete loss of enterokinase-inhibitory activity.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004765:Enteropeptidase / Q000037:antagonists & inhibitors*; D007887:Fabaceae / Q000032:analysis; D006801:Humans; D046911:Macromolecular Substances; D010434:Pepsin A; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D010944:Plants / Q000032:analysis*; D010946:Plants, Medicinal; D011480:Protease Inhibitors; D013552:Swine
D002241:Carbohydrates; D046911:Macromolecular Substances; D010940:Plant Proteins; D011480:Protease Inhibitors; D004765:Enteropeptidase; D010434:Pepsin A