The effect of calf serum on human lymphoblastoid cell-derived interferon production.
Substitution of low-cost calf serum for fetal bovine serum was evaluated in Namalva cell production of human alpha-lymphoblastoid interferon [HuIFN-alpha(Ly)] in 50-liter fermentors. Namalva cell growth and IFN yields were greatly reduced and protein precipitate weight increased from calf serum-supplemented cell cultures. The overall reduction in IFN production was greater than threefold, making the substitution impractical.
D000818:Animals; D001790:Blood Physiological Phenomena; D002417:Cattle; D002453:Cell Cycle; D002478:Cells, Cultured; D011232:Chemical Precipitation; D003362:Cost-Benefit Analysis; D003470:Culture Media; D005333:Fetus; D006801:Humans; D007158:Immunologic Techniques / Q000191:economics*; D007370:Interferon Type I / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D007370:Interferon Type I