The protein composition of the cytoplasmic membrane of aerobically and anaerobically grown Escherichia coli.
The protein composition of the cytoplasmic membranes of Escherichia coli, grown aerobically and anaerobically on a glucose minimal medium at pH 7.0, were analyzed by crossed immunoelectrophoresis. Qualitative differences are limited to only two proteins: nitrate reductase (E.C. 1.7.99.4) is absent under aerobic growth conditions, whereas an unidentified protein, with a molecular weight of 81,500 and located at the inner side of the cytoplasmic membrane, is synthesized only in the presence of oxygen. Quantitative differences are observed for many proteins: the ratio of the amount of a specific protein present in cells grown anaerobically and aerobically was, for four proteins, between 0.3 and 1; for 25 proteins, between 1 and 3; and for five proteins, larger than 5.
D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007123:Immunoelectrophoresis, Two-Dimensional; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D009565:Nitrate Reductases; D050901:Nitrate Reductase