Hydrogen peroxide as a source of molecular oxygen for in vitro mammalian CNS preparations.
Using an isolated neonatal rat spinal cord preparation, we have studied the ability of hydrogen peroxide to act as a source of oxygen for mammalian central nervous system (CNS) maintained in vitro. We report here that hydrogen peroxide, in low concentrations (0.001-0.004%), can effectively provide the only source, or act as a supplementary source of oxygen. This is brought about via the intracellular enzyme catalase which catalyzes the conversion of H2O2 into molecular oxygen and water.
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology; D006652:Histological Techniques; D006861:Hydrogen Peroxide / Q000494:pharmacology; D000860:Hypoxia / Q000517:prevention & control; D066298:In Vitro Techniques; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D012023:Reflex, Monosynaptic / Q000187:drug effects; D013116:Spinal Cord / Q000502:physiology*
D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D009584:Nitrogen; D010100:Oxygen