Recombinant mouse interferon-gamma regulation of antibody production.
Interferon-gamma produced in monkey cells by transfection with mouse interferon-gamma cDNA suppressed the mouse in vitro antibody response in a manner similar to that of natural mouse interferon-gamma. Significant suppression was obtained with as little as 1 U of interferon. Recombinant human interferon-gamma produced by cloning in a similar fashion was not suppressive. Both the suppressive and the antiviral activities of recombinant interferon-gamma were neutralized by antibodies to mouse natural interferon-gamma. Thus, interferon-gamma was responsible for the immunosuppression. At the cellular level, the recombinant interferon-gamma was capable of activating macrophages to suppress antibody production. The data provide clear-cut evidence that interferon-gamma plays an important role in regulation of immunological processes.
D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology; D004274:DNA, Recombinant / Q000276:immunology*; D005260:Female; D000882:Haplorhini; D006801:Humans; D007108:Immune Tolerance; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection
D004274:DNA, Recombinant; D007371:Interferon-gamma; D004247:DNA