Diamide mediated deaggregation of activated blood platelets is not caused by changes in adenine nucleotide pattern.
Studies on the effect of diamide (azodicarboxyl-bis-dimethylamide), a glutathione oxidizing agent, on platelet functions revealed an initial acceleration of aggregation followed by a pronounced deaggregation. The whole pattern of adenine nucleotides was followed up during both, arachidonic acid and arachidonic acid + diamide induced aggregation to prove whether the diamide mediated deaggregation is caused by changes in one of the parameters of the energy metabolism, i. e. ATP level, adenylate energy charge or ATP/ADP ratio.
D000227:Adenine Nucleotides / Q000097:blood*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001391:Azo Compounds / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D003958:Diamide / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects
D000227:Adenine Nucleotides; D001095:Arachidonic Acids; D001391:Azo Compounds; D003958:Diamide; D016718:Arachidonic Acid