Myeloid differentiation antigen defined by a monoclonal antibody IF10.
A monoclonal antibody of IgM class that defines the antigen present on human peripheral blood granulocytes was produced and characterized. This monoclonal antibody (IF10) was made from a single fusion between P3-X63-Ag8-U1 (P3U1) myeloma cells and splenocytes from a BALB/c mouse immunized against human cultured monocytoid cell line THP-1 cells. IF10-defined antigen(s) was expressed on the cells of granulocyte lineage such as peripheral blood granulocytes and metamyelocytes, myelocytes, promyelocytes, and a part of myeloblasts in the normal bone marrow, whereas it was not detected on resting and activated T lymphocytes, B lymphocytes, adherent monocytes, and thymocytes. The IF10-defined antigen was also expressed on cultured monocytoid cell lines as well as myeloid and myeloid/erythroid (K-562) cell lines. T lymphoblastoid cell lines, particularly those representing the T cells at an early thymocyte level, and a part of null cell lines were also reactive to IF10. Therefore, IF10 may be a unique monoclonal antibody that defines an antigen(s) which is expressed on almost whole stages of granulocytes and early stages of macrophages and T-cell lineages, and possibly during very early stages of erythroid lineage.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D001854:Bone Marrow Cells; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007938:Leukemia / Q000473:pathology; D008223:Lymphoma / Q000473:pathology; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D003165:Complement System Proteins