[Allogeneic transplantation of isolated islands of Langerhans into the liver of diabetic dogs with reference to its immunologic aspects].
By means of collagenase digestion and Ficoll gradient separation technique, viable islets of Langerhans were isolated from canine pancreases. These islets were transplanted into the liver of diabetic dogs. An experimental diabetes was induced by subtotal pancreatectomy and an application of 25 mg streptocotocin/kg bodyweight done twice. We performed the islet transplantation in several groups: 5 dogs differed from the donors by a strong histoincompatibility (EM-Test more than 15%). 5 dogs with a weak histoincompatibility and immunosuppression by Imuran and anti-thymocyte serum. The best results after transplantation were reached in the group with immunosuppression. There was an ameliorate effect on the hyperglycemia over a period of one year and we found significant differences in the glucose tolerance tests and in the immunoreactive insulin concentrations in contrast to the diabetic control dogs. No signs of portal hypertension and disturbance of the liver function were observed over the whole time of observations.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000601:surgery*; D004285:Dogs; D006649:Histocompatibility Antigens / Q000276:immunology; D007165:Immunosuppression Therapy; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000276:immunology; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000276:immunology / Q000601:surgery*; D008111:Liver Function Tests; D014181:Transplantation Immunology
D001786:Blood Glucose; D006649:Histocompatibility Antigens; D007328:Insulin
Allogene Transplantation isolierter Langerhansscher Inseln in die Leber diabetischer Hunde unter Berücksichtigung immunologischer Aspekte.