Selective inhibition of 5-lipoxygenase by natural compounds isolated from Chinese plants, Artemisia rubripes Nakai.
Three of four natural compounds, which are caffeic acid, eupatilin and 4'-demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5-lipoxygenase of cultured mastocytoma cells. Half-inhibition doses (ID50) for caffeic acid, eupatilin and 4'-demethyleupatilin were 3.7, 14 and 18 X 10(-6) M, respectively. The inhibition by caffeic acid was non-competitive types. Prostaglandin synthase activities were little inhibited by eupatilin and 4'-demethyleupatilin, but rather stimulated by caffeic acid. The formation of leukotriene C4 and D4 by mast tumor cells was almost completely suppressed by these compounds at 10(-4) M.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D002681:China; D002999:Clone Cells; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D013189:SRS-A / Q000096:biosynthesis; D012513:Sarcoma, Experimental / Q000201:enzymology
D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D013189:SRS-A; D001091:Arachidonate Lipoxygenases