A comparison of the effects of two gold-containing therapeutic agents on articular chondrocyte growth in vitro.
Chondrocyte structure and function under the influence of two gold-containing therapeutic agents, aurothioglucose and triethylphosphine gold, were studied in a monolayer culture system for cultivating lapine articular chondrocytes. The functional parameters investigated were chondrocyte proliferation and the incorporation of 35SO4 as indicator of glycosaminoglycan synthesis. Aurothioglucose (0.2, 1 and 10 micrograms/ml) failed to affect either parameter and caused no cytotoxic effect detectable with the light microscope. Triethylphosphine gold (1.5 and 3 micrograms/ml) prevented subcultured chondrocytes from forming monolayers and was cytotoxic to chondrocytes present in established monolayers, studied with the light and scanning electron microscope. A 0.3 microgram/ml concentration did not alter chondrocyte proliferation or sulphate incorporation and was not cytotoxic.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D006051:Aurothioglucose / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D006046:Gold / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D050607:Organogold Compounds; D009942:Organometallic Compounds; D010720:Phosphines; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits
D000893:Anti-Inflammatory Agents; D050607:Organogold Compounds; D009942:Organometallic Compounds; D010720:Phosphines; D011509:Proteoglycans; D006051:Aurothioglucose; C016797:triethyl phosphine gold; D006046:Gold; D004247:DNA