Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow.
Affinity-purified rabbit antibody to human lambda and kappa chains (R alpha H lambda kappa) was conjugated to the A-chain of the plant toxin, ricin. The resulting immunotoxin (R alpha H lambda kappa-A) killed cells from the tumor cell line Daudi, which bears surface immunoglobulin, but was nontoxic to the CFU-E, BFU-E, and CFU-GM of human bone marrow. R alpha H lambda kappa-A eliminated 99% of clonogenic Daudi cells that had been mixed with marrow cells in vitro, without demonstrable toxicity to hematopoietic cells. Thus, in vitro treatment of marrow with R alpha H lambda kappa-A may increase the incidence of cure following autologous bone marrow transplantation for the treatment of human B-cell malignancies.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000187:drug effects*; D001854:Bone Marrow Cells; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells; D006801:Humans; D007137:Immunoglobulin alpha-Chains / Q000378:metabolism; D012276:Ricin / Q000633:toxicity*
D000888:Antibodies, Anti-Idiotypic; D007137:Immunoglobulin alpha-Chains; D012276:Ricin