Patterns of nuclear lamins in diverse animal and plant cells and in germ cells as revealed by immunofluorescence microscopy with polyclonal and monoclonal antibodies.
Polyclonal and monoclonal antibodies against rat liver nuclear lamins have been used to evaluate the immunological cross-reactivity of lamins with a given antibody in a variety of animal and plant cells. The results indicated that lamins of all vertebrate cells but not invertebrate cells share at least one antigenic determinant, resulting in immunological cross-reaction with polyclonal antisera to lamina from rat liver. The range of cross-reaction with monoclonal antibody to rat lamins includes all mammalian cells tested but we observed no reaction with other vertebrate and invertebrate cells. Thus, by means of immunological cross-reaction a less stringently conserved pattern is observed for lamins than, for example, cytoskeletal proteins. We have also investigated the fate of the nuclear lamins during meiosis in testes and ovaries of the mouse. Lamins are absent from male meiotic cells and during oogenesis in meiotic prophases.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D034882:Lamins; D008297:Male; D009685:Nuclear Envelope / Q000648:ultrastructure*; D009698:Nucleoproteins / Q000276:immunology / Q000378:metabolism*; D009866:Oogenesis; D013091:Spermatogenesis; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D034882:Lamins; D009698:Nucleoproteins