Enzymological studies in chronic lymphocytic leukemia.
Adenosine deaminase (ADA), 5'nucleotidase (5'NT), ecto-5'NT, purine nucleoside phosphorylase (PNP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), adenine phosphoribosyltransferase (APRT), adenosine kinase (AK), AMP-deaminase (AMPD) and adenylate kinase (AdKin) activities were assayed in peripheral blood lymphoid cells from 20 patients with B-cell type chronic lymphocytic leukemia (CLL). Significantly decreased mean activities of ADA, 5'NT, ecto-5'NT, PNP and AMPD were observed when comparing B-CLL lymphoid cells with control peripheral blood lymphocytes (PBL). AK and AdKin activities however, were found to be higher in B-CLL. Relatively wide ranges of ADA and 5'NT activity were observed. In patients with paraproteinaemia, 5'NT activity was found to be relatively high and in the range of the activities in normal PBL. ADA activity seemed to be slightly higher in patients without paraproteinaemia. No correlation could be found between the enzyme activities and the number of cells rosetting with sheep erythrocytes or bearing surface immunoglobulin (sIg). A relationship was suggested between 5'NT activity and Ig production.
D000243:Adenosine Deaminase / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D010265:Paraproteinemias / Q000201:enzymology; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D012397:Rosette Formation
D011683:Purine-Nucleoside Phosphorylase; D000263:Adenylate Kinase; D009699:N-Glycosyl Hydrolases; D000243:Adenosine Deaminase