Protective effect of R-factor plasmid pKM101 on lethal damage by UV and chemical mutagens in E. coli strains with different DNA-repairing capacities.
The sensitivities of 8 R-factor plasmid pKM101-mediated E. coli strains with different DNA-repairing capacities to UV, AF-2 and MMS were investigated. The clear protective effect of pKM101 to lethal damage induced by these agents was only seen in the recA+ lexA+ and the uvr- lex- strains. The results support the hypothesis that the protective effect of pKM101 depends on the recA+ genotype. It is also possible to suggest that the product of pKM101 works not only on SOS repair which requires the recA and lexA gene functions but also on some unknown repair mechanisms.
D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005668:Furylfuramide / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000187:drug effects / Q000528:radiation effects; D005804:Genes, Lethal / Q000187:drug effects / Q000528:radiation effects; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D009581:Nitrofurans / Q000494:pharmacology*; D011815:R Factors; D013045:Species Specificity; D014466:Ultraviolet Rays
D009153:Mutagens; D009581:Nitrofurans; D005668:Furylfuramide; D008741:Methyl Methanesulfonate