HPLC analysis of E. coli rRNA on ASAHIPAK GS-520 and nucleobases, nucleosides and nucleotides on ASAHIPAK GS-320.
High-performance gel filtration chromatography using packed column Asahipak GS-520 was employed to separate E. coli 23S, 16S and 5S rRNAs. Low-molecular-weight components of nucleic acid were separated with Asahipak GS-320. Concurrent, rapid analysis of nucleobases, nucleosides and nucleotides was obtained isocratically. The elution of these substances is also described.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004926:Escherichia coli / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011108:Polymers; D011687:Purines / Q000302:isolation & purification*; D011743:Pyrimidines / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012263:Ribonucleosides / Q000302:isolation & purification*
D011108:Polymers; D011687:Purines; D011743:Pyrimidines; D012335:RNA, Ribosomal; D012263:Ribonucleosides