High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives.
A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003619:Dansyl Compounds / Q000302:isolation & purification*; D005453:Fluorescence; D005971:Glutamates; D008239:Lysine / Q000031:analogs & derivatives; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000302:isolation & purification; D009952:Ornithine / Q000031:analogs & derivatives
D003619:Dansyl Compounds; D005971:Glutamates; C000203:deoxyaminopteroic acid; D009952:Ornithine; D008239:Lysine; D008727:Methotrexate