Effects of monensin on biosynthesis and intracellular processing of proinsulin.
Monensin, a specific sodium ionophore, has been shown to reduce glucose-induced proinsulin biosynthesis by 30% and to completely inhibit the intracellular conversion of proinsulin to insulin. Autoradiography of monensin-treated cells demonstrated the presence of large quantities of newly synthesized proteins in amorphous vesicles close to the Golgi complex of B cells. The results suggest profound effects of monensin on biosynthesis and intracellular processing of proinsulin.
D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D005663:Furans / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008854:Microscopy, Electron; D008985:Monensin / Q000494:pharmacology*; D011384:Proinsulin / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D005663:Furans; D007457:Iodine Radioisotopes; D014316:Tritium; D011384:Proinsulin; D008985:Monensin