Circadian variation of lymphocyte subpopulations: a study with monoclonal antibodies.
Use of monoclonal antibodies to identify subpopulations of circulating lymphocytes in healthy adults showed pronounced circadian variations in total T cells, the two major T cell subsets, and HLA-DR+ lymphocytes. When the results for the T cell subsets were expressed as a ratio (helper:suppressor) no significant rhythmic variation was observed. Lymphocytes bearing a surface antigen identified by the HNK-1 antibody (a population containing the natural killer and antibody dependent killer activity) did not show significant rhythmic variation. There was an inverse relation between plasma cortisol concentration and numbers of T and B cells. These observations have therapeutic implications and should be considered in the course of immunological monitoring.
D000328:Adult; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes; D002940:Circadian Rhythm; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007958:Leukocyte Count; D008214:Lymphocytes; D008875:Middle Aged; D013601:T-Lymphocytes
D000911:Antibodies, Monoclonal; D006854:Hydrocortisone