Genetic analysis of a streptomycin-resistant oligosporogenous Bacillus subtilis mutant.
Strain SRB15T+, a streptomycin-resistant, oligosporogenous mutant of Bacillus subtilis, contains two mutations, fun and strR. These mutations were mapped by PBS-1 mediated transduction and by transformation to two different sites in the cysA-linked region of the B. subtilis chromosome. The fun mutation mapped very close to rpsLl, a classic strA mutation, whereas strR mapped to a site distal to rpsE. The effects of these mutations on growth, sporulation, and streptomycin resistance in vivo and in vitro were determined. The fun mutation gave a different phenotype than did the rpsLl mutation and caused altered migration of a ribosomal protein which was identified as S12, the protein encoded by rpsL. It therefore appears that fun is an allele of the rpsL gene.
D000483:Alleles; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004352:Drug Resistance, Microbial; D009154:Mutation; D010641:Phenotype; D013171:Spores, Bacterial / Q000502:physiology; D013307:Streptomycin / Q000037:antagonists & inhibitors*
D013307:Streptomycin