Demonstration of proline-rich proteins in rabbit parotid saliva and partial characterization of some of the proteins.
Rabbit parotid saliva was collected by cannulation of the secretory duct in anaesthetized animals. Proteins which cross-react with antibodies to human acidic proline-rich proteins were demonstrated in the secretion and fractionated by chromatography on an immunosorbent and CM32-cellulose. At least 5 proline-rich proteins were identified with molecular weights ranging from 19,000 to 61,000 as determined by sodium dodecylsulphate acrylamide gel electrophoresis. The major immunoreactive components were basic proteins with similar size and charge properties. In two of the proteins, proline, glycine and glutamic acid or glutamine accounted for 84 or 99 per cent of all residues. In contrast to proline-rich proteins from other species, proline constituted only 13 or 17 per cent of total amino acids.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005779:Immunodiffusion; D008970:Molecular Weight; D010306:Parotid Gland; D010455:Peptides / Q000032:analysis*; D055232:Proline-Rich Protein Domains; D011817:Rabbits; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D000596:Amino Acids; D010455:Peptides; D012471:Salivary Proteins and Peptides