[Mechanisms of interaction of acetylsalicylic acid and indomethacin with endoperoxide prostaglandin synthetase].
A study was made of the character and kinetics of interaction of acetylsalicylic acid and indomethacin with endoperoxide prostaglandin synthetase (PGH-synthetase) of sheep vesicular glands and human platelets. Enzymatic activity of PGH-synthetase was determined polarographically with the aid of Clark's electrodes. Acetylsalicylic acid was found to inhibit PGH-synthetase of sheep vesicular glands and human platelets at concentrations of the order of 1 x 10(-6) and 1 x 10(-4) M, whereas indomethacin at concentrations of 1 x 10(-6) and 1 x 10(-7) M, respectively. Acetylsalicylic acid inhibited PGH-synthetase from sheep vesicular glands and that from human platelets at an equal rate. Indomethacin inhibited the enzyme from sheep vesicular glands to a higher degree. Indomethacin reversibly interacted with PGH-synthetase. Meanwhile acetylsalicylic acid inhibited this enzyme irreversibly.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000201:enzymology; D016861:Cyclooxygenase Inhibitors; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep
D016861:Cyclooxygenase Inhibitors; D001241:Aspirin; D007213:Indomethacin
O mekhanizmakh vzaimodeĭstviia atsetilsalitsilovoĭ kisloty i indometatsina s éndoperoksidprostaglandinsintetazoĭ.