[Use of the protoplast fusion method in the selection of Streptomyces griseus--the producer of the streptothricin antibiotic grisin].
An effective method for protoplast fusion in S. griseus producing grisin was developed. The method requires the use of polyethylene glycol with a molecular weight of 1000. It was demonstrated that protoplasts formed most effectively in this organism, when the mycelium of the streptomycete previously treated with ultrasound in the process of its growth was used for the treatment with lysozyme. The efficacy of protoplast regeneration in the strains with the use of the modified hypertonic medium R2MD was 25-75 per cent. The possibility of using the protoplast fusion method for constructing phage resistant strains producing kormogrisin was shown.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D005821:Genetic Techniques; D005838:Genotype; D011523:Protoplasts / Q000378:metabolism*; D011995:Recombination, Genetic; D012641:Selection, Genetic; D013305:Streptomyces griseus / Q000235:genetics* / Q000378:metabolism; D013309:Streptothricins
D000900:Anti-Bacterial Agents; C005960:grisein; D013309:Streptothricins
Ispol'zovanie metoda sliianiia protoplastov v selektsii Streptomyces griseua--produtsenta streptotritsinovogo antibiotika grizina.