Effects of Mn2+, Ca2+ and chlorpromazine on photosystem II of Anacystis nidulans. An attempt to establish a functional relationship of amino acid oxidase to photosystem II.
Washing with EDTA changes the specificity of Anacystis nidulans particles having photosystem II activities for activation by cations. A specific requirement for Mn2+ and a somewhat lower specificity for Ca2+ can be demonstrated in the EDTA-washed particles. Both ions must be added to reconstitute the system evolving O2 in the light. EDTA-washed particles retain the L-amino acid oxidase with high specificity for the basic L-amino acids [Pistorius, E. K. and Voss, H. (1980) Biochem. Biophys. Acta, 611, 227-240] as well as the ability to reduce 2,6-dichloroindophenol with diphenylcarbazide as a donor in the light. The latter reaction which does not require added cations, can be inhibited by chlorpromazine, and this inhibition can be partially relieved by Ca2+ ions. Evidence is also presented that the L-amino-acid oxidase is inhibited by chlorpromazine, and this inhibition can be relieved by L-arginine in much the same way as the inhibition of the enzyme by Ca2+ ions can be relieved by L-arginine. The data are compatible with, but do not prove, an involvement of the L-amino-acid oxidase in the redox reactions of photosystem II of A. nidulans.
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D002746:Chlorpromazine / Q000494:pharmacology*; D000458:Cyanobacteria / Q000187:drug effects*; D004793:Enzyme Reactivators; D050830:L-Amino Acid Oxidase; D008345:Manganese / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000187:drug effects*
D004793:Enzyme Reactivators; D008345:Manganese; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D002118:Calcium; D002746:Chlorpromazine