Adherence of bacteria to pediatric intravenous catheters and needles and its relation to phlebitis in animals.
The adherence of bacteria to pediatric IV catheters and needles was studied. Scanning electron micrographs showed that bacteria adhered well to the catheters and needles, mainly to non-smooth surface areas. In vitro quantitative determination, with the use of radiolabeled bacteria, revealed differences in the affinity of bacteria for the various IV cannula materials. The adherence per square area was greatest for plastic catheters, less for steel needles, and least for siliconized needles. Mean values for the adherence of Staphylococcus aureus to these cannulae were 37.9-40.3 X 10(5) bacteria/cm2 for the plastic catheters; 10.2 X 10(5) bacteria/cm2 for the steel needles, and 7.2-7.6 X 10(5)/cm2 for the siliconized needles. Removal of the glutaraldehyde-fixed bacteria adhered to the cannulae, after their placement in veins of rabbits, was lower for the plastic catheters than the IV needles. The appearance and severity of venous phlebitis produced by the various cannulae was determined in an animal model. The degree of the inflammatory response elicited correlated with the in vitro bacterial adherence, indicating that bacterial adherence plays a role in the appearance of cannula-associated phlebitis. In view of our results and other previous observations of lower rate of infections with the use of IV needles, it is suggested that needles should be preferred to plastic catheters whenever possible. The described in vitro assay for bacterial adherence can be used to determine the adherent properties of IV cannulae, which should be considered in any future cannula design.
D000268:Adhesiveness; D000818:Animals; D002408:Catheters, Indwelling / Q000009:adverse effects; D002648:Child; D003428:Cross Infection / Q000382:microbiology*; D004926:Escherichia coli; D006801:Humans; D007263:Infusions, Parenteral / Q000295:instrumentation*; D007711:Klebsiella pneumoniae; D008297:Male; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D009339:Needles; D010689:Phlebitis / Q000209:etiology / Q000382:microbiology*; D011817:Rabbits; D013211:Staphylococcus aureus