[Lipopolysaccharide identification in aqueous extracts of Pseudomonas aeruginosa by an immunoenzyme method].
To determine the content of lipopolysaccharide (LPS) in P. aeruginosa aqueous extracts the ELISA was used. Membrane filtration and ultracentrifugation followed by precipitation with ammonium sulfate at 80% saturation and gel filtration on Sephadex G-100 have proved to be the most effective methods for purification of the aqueous extract from LPS.
D000942:Antigens, Bacterial / Q000032:analysis; D002056:Burns / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000032:analysis* / Q000276:immunology; D012996:Solutions
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D012996:Solutions
Identifikatsiia lipopolisakharida v vodnykh ékstraktakh Pseudomonas aeruginosa immunofermentnym metodom.