Use of the immuno-gold technique for in situ localization of cauliflower mosaic virus (CaMV) particles and the major protein of the inclusion bodies.
We show that the immuno-gold technique adapted to electron microscopy is a sensitive method for in situ localization of viral proteins in plant cells. Using antisera raised against cauliflower mosaic virus and the viroplasm major protein (VmP) we obtained a selective labelling of the viral particles and of the viroplasmic matrix in infected cells.
D006046:Gold; D007158:Immunologic Techniques; D007181:Inclusion Bodies, Viral / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D009029:Mosaic Viruses / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D014764:Viral Proteins; D006046:Gold