Intestinal alkaline phosphatase linked to immunoglobulin G of the kappa type.
An electrophoretically slow-moving alkaline phosphatase(orthophosphoric-monoester phosphohydrolase, EC 3.1.3.1, alkaline optimum, ALP) isoenzyme was found in the serum of a patient with osteomalacia. Molecular mass of this ALP was estimated to be 500 000 daltons. Inhibitor studies revealed that the abnormal ALP, which formed a macromolecular complex, was an intestinal isoenzyme. Immunofixation following electrophoresis demonstrated that the macromolecular ALP was a complex composed of intestinal ALP and immunoglobulin G of the kappa type. This isotype specificity of immunoglobulin suggested that the ALP immunoglobulin complex is a specific antigen-antibody complex in circulation.
D000368:Aged; D000469:Alkaline Phosphatase / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000276:immunology*; D008970:Molecular Weight; D010018:Osteomalacia / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D000469:Alkaline Phosphatase