Characterization of Brucella ovis lipopolysaccharide and its use for diagnosis of ram epididymitis by enzyme-linked immunosorbent assay.
Rough lipopolysaccharide, extracted by a mixture of phenol, chloroform, and petroleum ether from freeze-dried Brucella ovis cells with a yield of 0.71%, contained relatively small amounts of protein and nucleic acid contaminants as compared with lipopolysaccharides from other Brucellae. The crude lipopolysaccharide was suitable as a diagnostic antigen in an enzyme-linked immunosorbent assay for the sensitive and specific detection of ram epididymitis caused by B. ovis infection. In comparative serological tests, the enzyme-linked immunosorbent assay with B. ovis lipopolysaccharide gave better identification of infections and fewer false-negative results than the enzyme-linked immunosorbent assay with sonicated antigen or the complement fixation test.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D002002:Brucella / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D004823:Epididymitis / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides