Detection of neuronal uptake of [3H]dopamine in the rabbit celiac artery.
[3H]Dopamine ([3H]DA) is specifically taken up by slices of rabbit celiac artery. Corticosterone, a selective inhibitor of extraneuronal uptake processes, was found to reduce the uptake of the labelled catecholamine by about 30%. Desimipramine, an inhibitor of uptake processes in noradrenergic neurons, caused a decrease of [3H]DA uptake of about 35%. Benztropine, an inhibitor of uptake processes in dopaminergic neurons reduced [3H]DA uptake by about 30%. The neuronal uptake of [3H]DA was completely inhibited by chemical sympathectomy or by simultaneous incubation of the slices with desimipramine and benztropine. Norepinephrine had a competitive effect on [3H]DA uptake and benztropine inhibited [3H]norepinephrine uptake by 32%. The results are consistent with the existence of a double noradrenergic and dopaminergic innervation of the rabbit celiac artery.
D000818:Animals; D002445:Celiac Artery / Q000294:innervation* / Q000378:metabolism; D003891:Desipramine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D006892:Hydroxydopamines / Q000494:pharmacology; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D016627:Oxidopamine; D011817:Rabbits; D013563:Sympathectomy, Chemical
D006892:Hydroxydopamines; D016627:Oxidopamine; D003891:Desipramine; D004298:Dopamine; D009638:Norepinephrine