Occurrence of antigen BCG 60 in leprosy derived corynebacteria and other coryneforms.
Previous studies have shown that anti BCG 60 monoclonal antibody could recognize major antigen A 7 of M. leprae. In the present investigation we attempted to search the presence of the same antigen from the strains of Leprosy Derived Corynebacteria (LDC+), which were isolated from leprosy lesions and were supposed to be the cultivable form of the leprosy bacillus. A comparative study was equally performed on 7 strains of Corynebacteria which were isolated in France and had no relation with leprosy patients (LDC-). Utilizing the ELISA technique with an anti BCG 60 monoclonal antibody, it was found that all the three LDC+ strains contained an antigenic determinant common to M. leprae, however no LDC+ specificity was found as this antigenic determinant was also revealed on two LDC- strains.
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D003352:Corynebacterium / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007918:Leprosy / Q000276:immunology / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial