Sequences of the 5S rRNAs of Azotobacter vinelandii, Pseudomonas aeruginosa and Pseudomonas fluorescens with some notes on 5S RNA secondary structure.
Recently published alignments of available 5 S rRNA sequences have shown that a rigid base pairing pattern, pointing to the existence of a universal five-helix secondary structure for all 5 S RNAs, can be superimposed on such alignments. For a few species, the alignment and the base pairing pattern show distortions with respect to the large majority of sequences. Their 5 S RNAs may form exceptional secondary structures, or there may just be errors in the published sequences. We have examined such a case, Pseudomonas fluorescens, and found the sequence to be in error. The corrected sequence, as well as those of the related species Azotobacter vinelandii and Pseudomonas aeruginosa, fit perfectly in the 5 S RNA sequence alignment and in the five-helix secondary structure model. There exists comparative evidence for the frequent presence of non-standard base pairs at several points of the 5 S RNA secondary structure.
D001395:Azotobacter / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011551:Pseudomonas fluorescens / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity
D012335:RNA, Ribosomal