Investigation of two commercially available kits for determination of IgG and IgM to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA).
Enzyme-linked immunosorbent assay (ELISA) kits for determination of IgG and IgM to Toxoplasma gondii provided by Labsystems Oy, Finland, were tested on a number of human sera. It was found that the IgG kit agreed well with the dye test (DT) in differentiating positive and negative results. A deviation from a straight line relationship in the positive results obtained was considered due to the different antigens involved in the two tests. The correlation between the results obtained in the IgM kit and DS-ELISA was remarkably good. Only a small fraction of the false negative results due to high IgG titres and false positive results due to rheumatic factor, experienced in the indirect fluorescent antibody test, were apparent in the IgM kit. False positive results due to the presence of rheumatic factor could be corrected for by the use of an IgM confirmatory test kit. The influence of antinuclear factor was not apparent in any of the tests used in this work.
D000953:Antigens, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D014122:Toxoplasma / Q000276:immunology*
D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic