Purification of amyloglucosidase.
Amyloglucosidase (glucoamylase; EC 3.2.1.3) has been purified from the culture filtrates of Aspergillus candidus Link var. aureus using hydrophobic interaction chromatography and DEAE-cellulose treatment. The enzyme thus obtained has a specific activity of 329 units/mg protein with the overall recoveries between 70 and 75%. The process appears to be of industrial promise.
D001230:Aspergillus / Q000201:enzymology; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification*; D005959:Glucosidases / Q000302:isolation & purification*; D005998:Glycine / Q000031:analogs & derivatives
C021316:N-phenylglycine; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase; D005998:Glycine