No demonstrable association between HLA DR4 and in vitro collagen reactivity as determined by the production of leukocyte inhibition factor.
Eight HLA DR4 positive and 9 HLA DR4 negative healthy volunteers as well as 2 DR4 positive and 1 DR4 negative patients with rheumatoid arthritis (RA) were compared for leukocyte inhibition factor (LIF) production and lymphocyte proliferation in response to native and denatured collagen types II and I/III. Purified protein derivative (PPD) of tuberculin served as a positive control in the LIF assay and PPD and phytohaemagglutinin (PHA) in the lymphocyte proliferation test. No significant difference in responsiveness to collagen was detected between these two HLA groups. The reactivity to collagen was not affected by removal of OKT8+ lymphocytes. The present results are in conflict with earlier reports by Solinger et al. (1981) and Solinger & Stobo (1982). The reason for the discrepancy has not been resolved.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D003094:Collagen / Q000276:immunology*; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II; D006801:Humans; D066298:In Vitro Techniques; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D003094:Collagen