A simple method for purification of human monocytes and evaluation of IgG Fc-receptor-mediated phagocytosis.
A simple and reproducible, although not completely original method for purification of human monocytes and evaluation of IgG Fc-receptor-mediated phagocytosis has been set up and is described here. The purity of monocyte preparations obtained by the use of this method is quite elevated (over 95%), but the yield, although satisfactory for this purpose, is rather poor. As to the test of phagocytosis, a time-response curve using opsonized sheep erythrocytes showed that 10-min incubation gives the best and most reproducible results. A peroxidase reaction is suggested in order to obtain a better evaluation of erythrocytes within monocytes. The test has been proved suitable for evaluation of Fc-receptor function of monocytes in primary biliary cirrhosis, an immunological liver disease, and might be also useful in immune complexes-mediated conditions.
D000328:Adult; D002469:Cell Separation; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D011961:Receptors, Fc / Q000276:immunology*
D007074:Immunoglobulin G; D011961:Receptors, Fc