An enzymatic fluorometric assay for L-lysine in plasma and tissue.
A simple, specific, and reliable method has been developed for the determination of L-lysine in blood plasma and tissue. The L-lysine in the sample is decarboxylated enzymatically, and fluorescamine is added to a pentan-1-ol extract of the cadaverine formed. This produces a stable product which is measured fluorometrically.
D002103:Cadaverine / Q000302:isolation & purification*; D002262:Carboxy-Lyases; D003959:Diamines / Q000302:isolation & purification*; D005260:Female; D005450:Fluorescamine; D006801:Humans; D008239:Lysine / Q000032:analysis* / Q000097:blood; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D013050:Spectrometry, Fluorescence
D003959:Diamines; D005450:Fluorescamine; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine; D002103:Cadaverine