[beta-Lactamase-positive Neisseria gonorrhoeae. II. Localization of homologous regions between the 3.2 mdalton and 4.6 mdalton plasmids by DNA-DNA heteroduplex studies].
The difference between the 4.6 MDalton and 3.2 MDalton N. gonorrhoeae plasmids, both inducing penicillinase production, is examined by restriction endonuclease analysis, transcription-/translation studies and DNA-DNA heteroduplex analysis. One difference between those plasmids is that the 4.6 MDalton one carries a region with inverted repeats on both ends between coordinates 0.9 and 2.3. This region is an insertion sequence most probably. The length of the inverted repeats is 0.15 to 0.19 MDalton. An other difference between both the plasmids is that the 4.6 MDalton one, but not the 3.2 MDalton one, induces a protein of 10800 molecular weight.
D004269:DNA, Bacterial / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis*; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000032:analysis* / Q000201:enzymology / Q000235:genetics; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D010405:Penicillinase / Q000235:genetics*; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D004270:DNA, Circular; D009692:Nucleic Acid Heteroduplexes; D010405:Penicillinase; D001618:beta-Lactamases
beta-Laktamase-positive Neisseria gonorrhoeae. II. Lokalisation der Homologieregionen zwischen den 3.2 MDalton- und 4.6 MDalton-Plasmiden durch DNS-DNS-Heteroduplex-Untersuchungen.