pH-dependent binding of peroxidase-antiperoxidase /PAP/ immune complex to Fc receptors of macrophages.
Rat peritoneal macrophages were incubated at 4 degrees C in media of varying pH-s containing rat peroxidase-antiperoxidase /PAP/ immune complex. Two binding maxima were found at pH 5.5 and 7.0, resp. There was a sharp decrease in binding between 5.5 and 5.0. The peak at pH 7.0 was found to be trypsin-sensitive.
D000818:Animals; D000936:Antigen-Antibody Complex; D005260:Female; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011961:Receptors, Fc / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D011961:Receptors, Fc