Adriamycin and daunomycin induce interstrand DNA crosslinks in Hela S3 Cells.
By using a new mild procedure for detecting DNA crosslinks it has been shown that adriamycin and daunomycin are able to form interstrand DNA crosslinks in HeLa cells. This effect seems to be preceded by transformation of the parent antibiotics in the cell to active forms. In addition, interstrand DNA crosslinks formed by adriamycin and daunomycin were found to be temperature- and alkali-labile.
D000970:Antineoplastic Agents / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000378:metabolism*; D003630:Daunorubicin / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009564:Nitracrine / Q000494:pharmacology
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D008937:Mitomycins; D016685:Mitomycin; D009564:Nitracrine; D004317:Doxorubicin; D003630:Daunorubicin