A miniature flow cell designed for rapid exchange of media under high-power microscope objectives.
The design, fabrication and use of a flow cell that allows rapid displacement of media viewed by short working distance, high power objectives are described. The cell has a small internal depth (about 0.04 cm), small volume (about 0.05 ml), and is chemically inert. It has been tested extensively in studies of tethered bacteria.
D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques / Q000295:instrumentation*; D004867:Equipment Design; D008853:Microscopy / Q000295:instrumentation; D008904:Miniaturization; D009068:Movement