Replication of five bovine respiratory viruses in cultured bovine alveolar macrophages.
The replication of five bovine respiratory viruses in cultured bovine alveolar macrophages (BAM) was investigated. The bovine herpesvirus DN-599 strain did not cause cytophatic effect (CPE), extracellular virus and intracellular antigen were not demonstrated. Although a small number of bovine respiratory syncytial virions were consistently released and about 1 percent of the BAM were fluorescence antibody (FA) positive the virus caused no CPE. Infectious bovine rhinotracheitis virus caused CPE at an input multiplicity of about 0.5, and 5 percent of the BAM were FA positive. Both bovine parainfluenza-3 and bovine viral diarrhea viruses caused CPE, infective virions were released and considerable proportions of the BAM were FA positive.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D008264:Macrophages; D011650:Pulmonary Alveoli / Q000166:cytology; D014779:Virus Replication; D014780:Viruses / Q000254:growth & development*