Presence of prostaglandin D2, E2 and F2 alpha in rat pancreatic islets.
The presence of prostaglandin (PG) D2, PGE2 and PGF2 alpha was demonstrated in rat pancreas and pancreatic islets by specific radioimmunoassay and their contents were measured separately. The amounts of PGD2, PGE2 and PGF2 alpha in rat pancreas were 16.1 +/- 1.1, 9.48 +/- 2.05 and 4.43 +/- 0.62(2) ng/g tissue, respectively. The amounts of PGD2, PGE2 and PGF2 alpha in rat pancreatic islets were 427 +/- 66, 482 +/- 281 and 479 +/- 41 pg/mg protein, respectively. The syntheses of several PGs from PGH2, a common intermediate for PG production, in islet homogenates were also studied. PGH2 was rapidly transformed into PGD2, PGE2 and PGF2 alpha in almost equal proportion in islet homogenates, and the transformation were proportional to the amounts of the homogenates added in the reaction.
D000818:Animals; D015237:Dinoprost; D015232:Dinoprostone; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D015230:Prostaglandin D2; D011450:Prostaglandin Endoperoxides, Synthetic / Q000378:metabolism; D044262:Prostaglandin H2; D011453:Prostaglandins / Q000378:metabolism*; D011457:Prostaglandins D / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D011463:Prostaglandins H / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011450:Prostaglandin Endoperoxides, Synthetic; D011453:Prostaglandins; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D011463:Prostaglandins H; D044262:Prostaglandin H2; D015237:Dinoprost; D015232:Dinoprostone; D015230:Prostaglandin D2