Brain 5-hydroxytryptamine level, metabolism, and binding in E1 mice.
Inbred E1 mice are highly susceptible to convulsive seizures upon "throwing" stimulation. The strain is known to have an abnormal 5-hydroxytryptamine (5-HT) metabolism. In the study here 5-HT level, [14C]5-hydroxytryptophan (5-HTP) metabolism, MAO activity and [3H]5-HT receptor binding were examined in the cortex, brainstem and cerebellum. In the interictal period cortical and brainstem 5-HT level and [3H]5-HT receptor binding were significantly lower. In the same period cortical biosynthesized [14C]5-HT from [14C]5-HTP taken up was higher, and MAO activity was not changed. L-DOPA with MK486 induced a low threshold of seizures and decreased cortical 5-HT level. Abnormally functioning 5-HT neurones may exist in the E1 mouse cortex.
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D001923:Brain Chemistry; D002230:Carbidopa / Q000494:pharmacology; D004295:Dihydroxyphenylalanine / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008995:Monoamine Oxidase / Q000378:metabolism; D010812:Physical Stimulation; D012701:Serotonin / Q000032:analysis / Q000378:metabolism*
D012701:Serotonin; D004295:Dihydroxyphenylalanine; D006916:5-Hydroxytryptophan; D008995:Monoamine Oxidase; D002230:Carbidopa