Comparison of an agar-gel immunodiffusion test with other serological methods for differentiating Brucella infected from vaccinated cattle.
An agar-gel immunodiffusion test was compared with other serological tests for detection of antibodies in the sera of 48 cattle vaccinated with Brucella abortus strain 19. Two hundred forty-two sera were tested over a 12 month period, and addition of positive reactions for each test resulted in totals of 170 for the card test, 74 for the standard tube-agglutination test, 71 for the 2-mercaptoethanol test, 64 for the dithiothreitol test and 22 for the agar-gel immunodiffusion test.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002004:Brucella Vaccine / Q000008:administration & dosage*; D002003:Brucella abortus / Q000145:classification*; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D008297:Male; D012703:Serotyping / Q000379:methods / Q000662:veterinary; D013997:Time Factors
D000907:Antibodies, Bacterial; D002004:Brucella Vaccine