A simple radiometric in vitro assay for acetylcholinesterase inhibitors.
A radiometric method for screening acetylcholinesterase inhibitors has been described. The method is based on the production of [14C]carbon dioxide from the hydrolysis of acetylcholine. The inhibitory concentration at 50% (IC50) values for several known acetylcholinesterase inhibitors were in agreement with literature values. The new radiometric method is simple, inexpensive, and has the potential for automation.
D000109:Acetylcholine / Q000494:pharmacology; D000110:Acetylcholinesterase; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004593:Electrophorus; D066298:In Vitro Techniques; D010830:Physostigmine / Q000494:pharmacology; D011874:Radiometry / Q000379:methods
D002250:Carbon Radioisotopes; D002800:Cholinesterase Inhibitors; D002245:Carbon Dioxide; D010830:Physostigmine; D000110:Acetylcholinesterase; D000109:Acetylcholine