Partial purification of a thrombocytopoiesis stimulating factor present in the serum of thrombocytopenic rats.
Successive chromatographic procedures made it possible to isolate thrombocytopoietin from the serum of thrombocytopenic rats. The following steps were taken: DEAE-cellulose phosphate chromatography, Sephadex chromatography, exclusion chromatography on DEAE-Sephadex A-50 gel. The apparent molecular weight of thrombocytopoietin was about 48,000 daltons. Like erythropoietin, thrombocytopoietin is a glycoprotein, its molecular weight is similar. A single form is obtained only when the different purification steps last less than 10 days. Beyond that time, partial degradation may occur.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006410:Hematopoiesis; D011868:Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium; D012645:Selenomethionine / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013921:Thrombocytopenia / Q000097:blood*; D013926:Thrombopoietin / Q000302:isolation & purification*
D006023:Glycoproteins; D011868:Radioisotopes; D012967:Sodium Dodecyl Sulfate; D013926:Thrombopoietin; D012645:Selenomethionine; D012643:Selenium