Stimulation of sodium transport by toad skin incubated with natural derivatives of corticosterone and deoxycorticosterone.
The effects on sodium transport of several steroids physiologically secreted or possibly involved in pathological disorders were compared with those of aldosterone in the isolated toad skin. The 18-hydroxylated derivatives of deoxycorticosterone and corticosterone, in contrast to their parent compounds, significantly enhanced sodium transport at a concentration of 50 nmol/l. In the presence of glucose, 18-hydroxydeoxycorticosterone increased transepithelial potential difference, as did aldosterone. The 19-nor derivative of deoxycorticosterone, recently implicated in the aetiology of adrenal regeneration hypertension, stimulated sodium transport, unlike 19-nor-corticosterone and 16-oxo-androstenediol. Insulin significantly increased sodium transport in aldosterone-treated skin and lowered the resistance. The natriferic response to vasopressin was potentiated fivefold by exposure of the skin to aldosterone and was doubled in skin exposed to 19-nor-deoxycorticosterone. We conclude that 18-hydroxylated adrenocortical steroids can play a physiological role in salt retention; furthermore, these steroids, as well as 19-nor-deoxycorticosterone, could be involved in pathological conditions such as low renin hypertension. Caution should be exercised in evaluating mineralocorticoid potency solely in terms of the urinary sodium to potassium ratio.
D015070:18-Hydroxydesoxycorticosterone / Q000494:pharmacology; D000450:Aldosterone / Q000494:pharmacology*; D000734:Androstenediols / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002024:Bufo marinus; D003345:Corticosterone / Q000494:pharmacology*; D046508:Culture Techniques; D003900:Desoxycorticosterone / Q000031:analogs & derivatives / Q000494:pharmacology*; D004594:Electrophysiology; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D015070:18-Hydroxydesoxycorticosterone; D000734:Androstenediols; C007421:3 beta,17 beta-dihydroxyandrost-5-en-16-one; D003900:Desoxycorticosterone; C019026:19-nordeoxycorticosterone; D000450:Aldosterone; D012964:Sodium; D003345:Corticosterone