[Collagen gel culture of human thyroid cancers].
When seeded on collagen gel surface, human thyroid cells from normal tissue or carcinoma form a monolayer. When the isolated cells are embedded inside the collagen gel, they reorganize into three-dimensional structures. The cells isolated from normal tissue, adenoma, or well-differentiated follicular carcinoma show the typical arrangement of follicular thyroid cells: the apical poles, characterized by microvilli, are oriented towards the follicular lumen; intercellular junctions are present. In contrast, in the papillary and moderately-differentiated follicular carcinomas, the cells are hardly ever associated, and ultrastructural nuclear anomalies are often observed.
D000236:Adenoma / Q000648:ultrastructure*; D000818:Animals; D002277:Carcinoma / Q000648:ultrastructure*; D002291:Carcinoma, Papillary / Q000648:ultrastructure*; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003584:Cytological Techniques; D005782:Gels; D006801:Humans; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D051381:Rats; D013961:Thyroid Gland / Q000648:ultrastructure; D013964:Thyroid Neoplasms / Q000648:ultrastructure*
D005782:Gels; D003094:Collagen
Culture en gel de collagène de cancers thyroïdiens humains.