Lewis-like activities of and rabbit antisera against glycoconjugates present in edible organs of phanerogamia and cryptogamia.
13 species of phanerogamia and cryptogamia were found to contain substances cross-reacting with anti-Lewis agglutinins in ethanol precipitates from their edible organs such as leaves. Crude glycoconjugates were isolated from some species by Sephacryl S-200 gel filtration, immunization with some of which resulted in the production in rabbits of anti-Lea-like agglutinins with a high titer. Production of anti-Lea-like precipitin with high specificity was also confirmed in a rabbit antiserum against the glycoconjugate prepared from young leaves of Aralia elata.
D000818:Animals; D003429:Cross Reactions; D000431:Ethanol; D006001:Glycoconjugates / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera; D007983:Lewis Blood Group Antigens / Q000276:immunology; D010936:Plant Extracts / Q000276:immunology; D010945:Plants, Edible / Q000032:analysis / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D013045:Species Specificity
D006001:Glycoconjugates; D007106:Immune Sera; D007983:Lewis Blood Group Antigens; D010936:Plant Extracts; D000431:Ethanol