Measurement of binding constants for divalent antibody-immobilized antigen interaction by antigen-sepharose 4B chromatography.
Binding constants for the human IgG-anti human IgG heavy chain were determined from elution profiles obtained by loading human IgG-Sepharose 4B columns with the antibody and eluting it at different NaI concentrations. The agreement between the value obtained at zero NaI concentration and the value determined in solution by equilibrium molecular sieving was good. An application of this column method should be useful for comparison of avidity values of antibody populations of the same specificity.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D012756:Sheep
D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains