The reaction of fluorescein isothiocyanate with thiols: a method for assay of isothiocyanates.
The reversible formation of dithiourethanes from fluorescein isothiocyanate and mercaptoethanol or mercaptoethylamine has been studied. It is shown that it is possible to use the reaction as the basis for a spectrophotometric titration of a number of isothiocyanates with mercaptoethanol.
D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D008623:Mercaptoethanol; D008624:Mercaptoethylamines; D013053:Spectrophotometry / Q000379:methods; D013861:Thiocyanates / Q000032:analysis*
D005452:Fluoresceins; D008624:Mercaptoethylamines; D013861:Thiocyanates; D008623:Mercaptoethanol; D016650:Fluorescein-5-isothiocyanate