[Study on renal transplantation. IX. Experimental study on kidney preservation with perfluorochemical emulsion at low temperature].
The unilateral kidney of mongrel dog was removed and flushed out with modified Collins C3 solution, and then preserved by pulsatile perfusion machine (MOX-100) at low temperature of 4-6 degrees C. During the perfusion, systolic pressure was controlled below 60 mmHg. After a 48-hr or 72-hr preservation, the kidney was autotransplanted to the iliac fossa of the same dog. The perfusate consisted of cryoprecipitated plasma (CPP) of the dogs supplemented with or without 5 W/V% perfluorochemical emulsion (FC-43). At an early stage, our CPP formed fibrin mass easily, thus being very unstable. But silicagel treatment of the CPP improved stability. Twenty six kidneys were perfused for preservation, but perfusion of 7 kidneys was stopped by decreased perfusion flow and increased perfusion pressure. Eight kidneys were lost due to technical failure of transplantation or infection after autotransplantation; 11 kidneys were used as planned. The 48-hr preservation group contained 2 cases of FC (+) and 2 cases of FC (-). Each group had one survival case. The 72-hr preservation group contained 6 cases of FC (+), and one case of FC (-). Three cases survived in the FC (+) group and one case survived in the FC (-) group. Survival cases maintained enough perfusion flow which was above 0.3 ml/min. g. kidney. Weight gains of the preserved kidneys during 72-hr preservation were 13 +/- 19% in FC (+) and 21 +/- 17% in FC (-) group, but had no relation to preservation condition.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D003080:Cold Temperature; D003404:Creatinine / Q000097:blood; D004285:Dogs; D004655:Emulsions; D005466:Fluorocarbons / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology / Q000473:pathology; D016030:Kidney Transplantation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010477:Perfusion; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival
D004655:Emulsions; D005466:Fluorocarbons; C008807:perfluorotributylamine; D003404:Creatinine; D007770:L-Lactate Dehydrogenase