Evaluation of the mutagenic potential of the hair dye N-methyl-amino-2-nitro-4-N',N'-bis-(2-hydroxyethyl)-aminobenzene in a battery consisting of Drosophila and mammalian cytogenetic assays.
The compound N-methyl-amino-2-nitro-4-N', N'-bis(2-hydroxyethyl)-aminobenzene was tested for mutagenic activity in the sex-linked recessive lethal test with Drosophila melanogaster, the induction of chromosomal aberrations and sister-chromatid exchanges (SCEs) with Chinese hamster ovary (CHO) cells in vitro, and the micronucleus test with mouse bone-marrow cells in vivo. Consistently negative results were obtained with the 3 tests. The SCE tests gave positive results with prolonged treatments. It is concluded that reliable decisions about mutagenic activity cannot be based on the induction, in vitro, of SCEs alone.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004331:Drosophila melanogaster / Q000187:drug effects*; D005260:Female; D005804:Genes, Lethal / Q000187:drug effects*; D005808:Genes, Recessive / Q000187:drug effects*; D006202:Hair Dyes / Q000494:pharmacology*; D006203:Hair Preparations / Q000494:pharmacology*; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D010053:Ovary; D010655:Phenylenediamines / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D006202:Hair Dyes; D006203:Hair Preparations; D009153:Mutagens; D010655:Phenylenediamines; C007906:N-methylamino-2-nitro-4-N',N'-bis(2-hydroxyethyl)aminobenzene