Cell-mediated and humoral alloimmunreactions after subperiostal implantation of allogenic demineralized dentin in humans.
13 patients suffering from local atrophy or loss of the alveolar process of the jaws were treated by subperiosteal implantation of allogenous, decalcified, sterilized dentin with the purpose of augmentation of the alveolar process. All patients, including 9 donors of dentin were ABO-, Rh-, and HLA tissue typed. Anti-donor cell-mediated immunity (CMI) and humoral-mediated immunity (HMI) of the patients were monitored during the first 6 postoperative months. The CMI and HMI were assessed by means of the leucocyte migration test (LMT) and the complement dependent microlymphocytotoxicity test (CDC) with donor leucocytes as antigen and target cells, respectively. Compared to 66 normal non-transplanted controls, the leucocyte migration inhibition against desintegrated donor leucocytes as antigen was significantly increased for 2 of the recipients during the control period. 1 of these recipients showed a simultaneous HMI reaction 6 weeks after implantation. The remaining CMI and HMI values of the recipients were within normal range. These results were not correlated with the histocompatibility match grades of the donor recipient pairs. The present investigation supports the suggestion that implantation of allogenous, decalcified and sterilized dentin occasionally is capable of eliciting a measurable alloimmune response.
D000328:Adult; D000368:Aged; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000917:Antibody Formation; D002464:Cell Migration Inhibition; D003601:Cytotoxicity Tests, Immunologic; D003804:Dentin / Q000637:transplantation*; D005260:Female; D006650:Histocompatibility Testing; D006801:Humans; D007111:Immunity, Cellular; D007962:Leukocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013516:Oral Surgical Procedures, Preprosthetic / Q000379:methods*; D013048:Specimen Handling; D014181:Transplantation Immunology