Further studies of the use of cycloheximide for cultivating Mycobacterium lepraemurium in cell culture.
The advantage of using cycloheximide for cultivating Mycobacterium lepraemurium in cell culture was further demonstrated. Continuous multiplication of the bacillus in successive subcultures was obtained in MFP, HEp-2 and Vero cells maintained in culture medium containing 0.1 microgram of cycloheximide per ml. Growth characteristics were comparable to those observed in the cultures of A31 cells previously reported. The procedure was simple and convenient. Comparable results, however, have not been obtained in cultures of other established cell lines, HeLa 229, L, MDCK, and Neuro-2a.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology*; D006801:Humans; D009167:Mycobacterium lepraemurium / Q000187:drug effects* / Q000254:growth & development
D003470:Culture Media; D003513:Cycloheximide