Coating of polymeric surfaces for immunoassay by a forced adsorption technique.
A forced adsorption technique for coating polymeric surfaces is described. This technique is simple and allows us to use (i) relatively impure ligands to be coated on latex surface and (ii) the minimum amount of ligands for preparation of large-volume lots of latex reagent. The serologic results are highly reproducible from lot to lot of the reagent, in contrast to the variability found with passively adsorbed reagents. Use of this technique could be extended to coat flat surfaces for solid-phase immunoassays.
D000327:Adsorption; D000907:Antibodies, Bacterial / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007840:Latex; D012284:Rickettsia rickettsii / Q000276:immunology; D012373:Rocky Mountain Spotted Fever / Q000276:immunology
D000907:Antibodies, Bacterial; D007840:Latex