Calcium exerts an indirect effect on ATP-dependent proteolysis of rat liver mitochondria.
The ATP-dependent proteolysis of rat liver mitochondria prepared in electrolyte-poor sucrose media requires the presence of Ca2+. Lanthanum, an inhibitor of Ca2+ uptake, inhibits the proteolysis. In contrast, proteolysis of mitochondria prepared in a salt medium does not require Ca2+, nor is it inhibited by lanthanum. It is concluded that Caa+ exerts its effect in an indirect manner, by causing swelling and thereby increasing the accessibility of the membrane proteins of the inner mitochondrial membrane.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats
D004533:Egtazic Acid; D007811:Lanthanum; D000255:Adenosine Triphosphate; D010447:Peptide Hydrolases; D002118:Calcium