A method for the preparation of large intact sheets of intestinal mucosa: application to the study of mouse aggregation chimeras.
A technique for the preparation of entire intestinal mucosal sheets is described that renders the population of crypts accessible for two-dimensional study. We have applied the technique to demonstrate the mosaic crypt populations in the intestinal epithelium of mouse aggregation chimeras, using the lectin Dolichos biflorus agglutinin (DBA) as a strain-specific histochemical marker.
D000818:Animals; D002678:Chimera; D006652:Histological Techniques; D007413:Intestinal Mucosa; D037102:Lectins; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D037121:Plant Lectins
D037102:Lectins; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin