[Enzymatic synthesis of tRNA fragments].
In the present paper the results of enzymatic synthesis of yeast tRNA1Val fragments have been summarized. It is shown that complex use of nucleolytic enzymes is a convenient and effective method of synthesis of the defined sequence oligoribonucleotides. The consecutive use of different nucleolytic enzymes (ribonucleases with different substrate specificity and polynucleotide phosphorylase) and RNA ligase has permitted to obtain various fragments (or their analogs) of T psi-loop, D-arm, anticodon arm and acceptor stem. Some fragments containing modified nucleosides such as tetranucleotide GpDpCpGp (fragment 15-18), octanucleotide GpUpCpUpApGpDpC (analog of fragment 10-17), nonanucleotide GpTpUpCpGpApUpCpC (analog of T psi-loop), decanucleotide psi pCpUpGpCpUpUpIpApC (analog of fragment 27-36), hexanucleotide CpApCpGpCpA (fragment 36-41) and others were synthesized.
D000926:Anticodon; D001483:Base Sequence; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000096:biosynthesis; D012331:RNA, Fungal / Q000096:biosynthesis* / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000096:biosynthesis* / Q000235:genetics; D012260:Ribonucleases / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism
D000926:Anticodon; D009841:Oligonucleotides; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D012260:Ribonucleases
Fermentativnyĭ sintez fragmentov transportnykh ribonukleinovykh kislot.