Expression of hepatitis B surface antigen in mammalian cells and identification of a precursor form of HBsAg.
The purpose of this study was to program the synthesis of hepatitis B surface antigen in cultured mammalian cells from defined DNA sequences derived from the Hepatitis B viral DNA. The product of this system used in the present study is the 22-nm hepatitis B surface antigen particles released in the culture medium with biophysical properties identical to the 22-nm HBsAg particles found in the serum of hepatitis B carriers. Additionally, preliminary results are presented concerning the synthesis of a precursor polypeptide bearing hepatitis B surface antigenic determinants.
D000818:Animals; D000882:Haplorhini; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic
D006514:Hepatitis B Surface Antigens