Immunochemical evidence for two 3-methylcholanthrene-inducible forms of cytochrome P-448 in rat liver microsomes using a double-antibody radioimmunoassay procedure.
Double-antibody radioimmunoassay (RIA) procedures were developed for the determination of two forms of cytochrome P-448 in crude liver microsomes at the picogram level. The antisera used in the RIAs were produced against two forms of cytochrome P-448 isolated from livers of rats treated with 3,4,5,3',4',5'-hexachlorobiphenyl (3,4,5-HCB) and 3-methylcholanthrene (3-MC), referred to as cytochromes P-448HCB and P-448MC, respectively. The results obtained with RIA procedures were consistent with results of radial immunodiffusion analysis, sodium dodecyl sulfate/polyacrylamide gel electrophoresis, and estimation of aryl hydrocarbon hydroxylase (AHH) activity. Utilizing the RIAs, both forms of cytochrome P-448 were found in significant quantities in liver microsomes of rats treated with 3-MC, 3,4,5-HCB, isosafrole, and Aroclor 1254 but only in minute concentrations in untreated and phenobarbital-treated rats.
D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D020111:Chlorodiphenyl (54% Chlorine); D019388:Cytochrome P-450 CYP1A2; D003580:Cytochromes / Q000032:analysis*; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D001140:Aroclors; D003580:Cytochromes; D020111:Chlorodiphenyl (54% Chlorine); D008748:Methylcholanthrene; D011078:Polychlorinated Biphenyls; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; C027309:3,4,5,3',4',5'-hexachlorobiphenyl; D010634:Phenobarbital