Identification of a rapidly labelled 350K histidine-rich protein in neonatal mouse epidermis.
The major histidine-rich protein (HRP) found in the stratum corneum of neonatal mouse epidermis (band 2 protein, molecular weight 27,000) is a relatively late product of epidermal differentiation and incorporates labelled amino acids in vivo only after a 6-9 h lag period. A number of putative precursor HRPs in the 70-300 K molecular weight range were initially identified using short pulse labeling times and our previously described methods for isolation of epidermis and extraction of proteins. However, when steps were taken to minimise proteolysis during preparation, a single species of approximately 350 K molecular weight was the most strongly labelled protein following a 1 h in vivo pulse of [3H]-histidine. This protein was stable in sodium dodecyl sulphate dithiothreitol at 100 degrees C and in 4 M urea, suggesting a single covalently linked polypeptide. The kinetics of labelling and the localisation of the 350 K HRP in the lower granular layers suggest that it is a precursor of the stratum corneum HRP. The processing of the 350 K HRP to the stratum corneum species appears to involve a complex series of specific cleavage steps which give rise to a number of HRPs of intermediate molecular weight.
D000818:Animals; D000831:Animals, Newborn; D004492:Edetic Acid / Q000494:pharmacology; D004817:Epidermis / Q000032:analysis / Q000378:metabolism*; D000091344:Filaggrin Proteins; D006639:Histidine / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism*
D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D011480:Protease Inhibitors; D011498:Protein Precursors; D006639:Histidine; D010664:Phenylmethylsulfonyl Fluoride; D004492:Edetic Acid