The use of cationic polyelectrolytes in the preparation of cell monolayers for automated cell scanning and diagnostic cytopathology.
The introduction of cationic polyelectrolytes as cellular adherents has significantly advanced the preparation of cervical scrape specimens for automated cell scanning and has also provided an efficient technique for the preparation of cell monolayers of other cytologic specimens, e.g., breast cyst fluids, urines and serous effusions, for diagnostic cytopathology. Variable thickness of cell preparation and cell overlap have both been resolved by laying cells onto glass slides coated with the cationic polyelectrolyte poly-L-lysine. We have determined the optimal conditions for pH, molecular weight, concentration and temperature for the application of poly-L-lysine as a cell-to-slide adhesive.
D002448:Cell Adhesion; D003584:Cytological Techniques; D001330:Electronic Data Processing; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010338:Pathology, Clinical / Q000379:methods*; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D011095:Polyethylenes; D011107:Polylysine; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013696:Temperature
D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D011095:Polyethylenes; D011107:Polylysine; D011108:Polymers; D000644:Quaternary Ammonium Compounds