13C NMR studies of glycolysis in intra- and extra-erythrocytic Babesia microti.
Metabolism in the erythrocytes of normal mice and mice infected with Babesia microti has been monitored non-invasively by high resolution 13C NMR spectroscopy. The conversion of [U-13C]glucose to lactate in both normal and infected cells together with the effect of the trypanocidal drug, 4,4'-diamidinodiazoaminobenzene diaceturate, on glycolytic rates were monitored. These studies show that erythrocytes utilize [U-13C]glucose at a rate of 3 X 10(-12) mumol cell-1 min-1 at 35 degrees C while parasitized cells consume 2.9 X 10(-11) mumol cell-1 min-1 and produce lactate as the sole end-product. This rate decreases to 9 X 10(-12) mumol cell-1 min-1 on the addition of 0.75 mM drug.
D000818:Animals; D001403:Babesia / Q000378:metabolism*; D001404:Babesiosis / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology; D005260:Female; D005990:Glycerol / Q000378:metabolism; D006019:Glycolysis; D006790:Host-Parasite Interactions; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei / Q000378:metabolism; D013045:Species Specificity
D005990:Glycerol