Expression of the Spiroplasma citri spiralin gene in Escherichia coli. Use of the recombinant plasmid carrying this gene as a molecular probe.
Expression in Escherichia coli of the cloned Spiroplasma citri spiralin gene results in the expression of a 30.5-kDa protein serologically related to spiralin. A protein with the same properties is also present in minor amounts in S. citri cells, suggesting that spiralin is first produced as a preprotein containing a signal polypeptide, which is removed during further processing. Hybridization experiments have demonstrated that pES1, the recombinant plasmid carrying the spiralin gene, can be used as a molecular probe, allowing the detection of S. citri DNA in infected plants and in insect cell cultures.
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008970:Molecular Weight; D010957:Plasmids; D013149:Spiroplasma / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; C028588:spiralin