[Regulation of the enzyme activity of para-xylene catabolism in a spontaneous para-xylene-negative variant of Pseudomonas aeruginosa 2x79].
A spontaneous variant of Pseudomonas aeruginosa 2x unable to grow on p-xylene as the sole source of carbon and energy has been isolated. p-Xylenenegative variant of P. aeruginosa 2x79 differs from the wild type strain by the character of growth on the p-xylene oxidation intermediates p-toluate and protocatochuate. The cell of 2x79 variant inability to grow on p-xylene has been shown to be accompanied by the elimination of the activities of three enzymes - p-xylene methylhydroxylase, p-cresol methylhydroxylase and metapyrocatechase and by the considerable alteration in the regulation of orto-cleavage aromatic ring enzymes activity pyrocatechase and protocatechuate-3,4-dioxygenase. Possible reasons for appearing spontaneous variants 2x79 in the population of P. aeruginosa 2x growing on the p-xylene are being discussed.
D001565:Benzoates / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004790:Enzyme Induction; D004794:Enzyme Repression; D014644:Genetic Variation; D062385:Hydroxybenzoates / Q000378:metabolism; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D014992:Xylenes / Q000378:metabolism*
D001565:Benzoates; D003470:Culture Media; D062385:Hydroxybenzoates; D014992:Xylenes; C009091:protocatechuic acid; C031286:4-xylene; C009504:4-toluic acid
Reguliatsiia aktivnosti fermentov katabolizma para-ksilola u spontannogo para-ksilolnegativnogo varianta Pseudomonas aeruginosa 2x79.