Ligand-induced interconversions of maize homoserine dehydrogenase among different states.
The threonine-sensitive homoserine dehydrogenase has been isolated and extensively purified from shoots of Zea mays L. var. earliking. This enzyme is shown to be hysteretic under certain conditions. Progress curves of the NAD-dependent reaction catalyzed by the maize enzyme can be characterized by distinct lags prior to achievement of steady state velocities, reflecting transitions from less active species to a more active steady state form of the enzyme. Incubation of the enzyme for 1 min at 25 degrees C prior to initiation of the reaction profoundly influences the properties of the less active enzyme and the nature of the subsequent slow transitions during assay. When the feedback modifier, L-threonine, or KCl is included in the preincubation mixture, the transitions involve biomolecular association reactions. In the absence of either ligand, or in the presence of an appropriate mixture of both, a unimolecular transition occurs during assay. Three unique preincubation states of the enzyme have been identified on the basis of their response to substrates and effectors; whereas, the kinetic and regulatory properties of the steady state form of the enzyme are independent of preincubation conditions. Steady state can thus be achieved by three different transitions. Each transition is retarded by threonine and favored by substrates and potassium, although the effects of these compounds differ quantitatively. Under the conditions tested, monovalent cations have no effect on the steady state velocity of the enzyme. A model describing the relationships among the four unique states of the enzyme which is consistent with the present results and supported by previous observations is proposed.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002384:Catalysis; D004789:Enzyme Activation / Q000187:drug effects; D006715:Homoserine Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands / Q000494:pharmacology*; D008956:Models, Chemical; D013912:Threonine / Q000494:pharmacology; D003313:Zea mays / Q000201:enzymology
D008024:Ligands; D013912:Threonine; D000429:Alcohol Oxidoreductases; D006715:Homoserine Dehydrogenase