Fluorescent cytochemical oestrogen receptor assay: is it valid in breast cancer?
The performance of a fluorescent cytochemical oestrogen receptor (ER) assay has been compared with an established dextran-coated charcoal ER assay in 85 cases of primary breast cancer. Both assays were performed on tissue samples from the same tumour, by independent observers in different laboratories. A good qualitative correlation between the two methods was found, with agreement in 92 per cent of cases (P less than 0.0005). Criticisms of the fluorescein-labelled oestrogen methods are discussed. It is concluded that such methods should be validated in each laboratory by comparison with a conventional, quantitative ER assay before being used for clinical decisions.
D000327:Adsorption; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000032:analysis*; D002606:Charcoal; D004958:Estradiol / Q000031:analogs & derivatives; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006651:Histocytochemistry; D006801:Humans; D008722:Methods; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000032:analysis*; D012710:Serum Albumin, Bovine; D013861:Thiocyanates
D005452:Fluoresceins; D011960:Receptors, Estrogen; D013861:Thiocyanates; D002606:Charcoal; D012710:Serum Albumin, Bovine; C002912:estradiol-6-(O-carboxymethyl)oxime; D004958:Estradiol; D016650:Fluorescein-5-isothiocyanate