[Survival of motile spermatozoa in a medium based on lactate, glucose and phosphate].
Suspensions of motile human spermatozoa, plasma seminal-free, were incubated several days into synthetic medium (C2) with lactate, glucose and phosphate as principal components. Maintenance of forward motility was compared in C2 medium and in known B2 I.N.R.A. medium. C2 medium entertain survival of motile spermatozoa during some days at 20 degrees C. This method of conservation should allow many homologous artificial inseminations by ovulatory cycle avoiding injurious effects of repeated ejaculations or low-temperature conservation.
D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D008297:Male; D010710:Phosphates / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*
D003470:Culture Media; D007773:Lactates; D010710:Phosphates; D019344:Lactic Acid; D005947:Glucose
Survie des spermatozoïdes mobiles dans un milieu à base de lactate, glucose et phosphate.