Measurement of the rate of substrate cycling between fructose 6-phosphate and fructose 1,6-bisphosphate in skeletal muscle by using a single-isotope technique.
The effects of several agents on the rates of the fructose 6-phosphate/fructose 1,6-bisphosphate substrate cycle were measured in incubated epitrochlearis muscles of the rat by monitoring the transfer of radiolabel from [6-14C]glucose to the 1-position of glucose residues in glycogen. The cycling rates observed were almost identical with those previously obtained by using the well-established dual-isotope technique. In particular, it was found that the beta-adrenoceptor agonist isoprenaline increased the cycling rate about 12-fold.
D000818:Animals; D002250:Carbon Radioisotopes; D005635:Fructosediphosphates / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006598:Hexosediphosphates / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008722:Methods; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D005635:Fructosediphosphates; D005636:Fructosephosphates; D006598:Hexosediphosphates; D007328:Insulin; C027618:fructose-6-phosphate; D011433:Propranolol; D005947:Glucose; D007545:Isoproterenol; C029063:fructose-1,6-diphosphate