Regional blood-flow measurement in rabbit soft-tissue tumor with positron imaging using the C15O2 steady-state and labeled microspheres.
The C15O2 steady-state method, with tomographic measurement of H215O, has been used to measure blood flow in normal muscle tissue and a soft-tissue tumor (V2) in rabbits. the method has been validated in a rabbit tumor model by comparison of the data with that observed using microspheres labeled with Sc-46 and Ga-68. The blood-flow values found with the C15O2 steady-state method, after correction for tissue blood volume, agree well with values gained with labeled microspheres. The effect of blood volume on apparent blood flow in tumor was about 50%. The tissue/blood partition coefficients were 0.94 +/- 0.05 for healthy tissue and 1.16 +/- 0.15 for tumor tissue. After correction for partition coefficient and blood-volume effects, the blood-flow value for normal tissue was 4.1 +/- 1.3 ml/min-100 cc, and for the tumor tissue 10.7 +/- 2.3 ml/min-100 cc.
D000818:Animals; D002245:Carbon Dioxide; D005069:Evaluation Studies as Topic; D005710:Gallium Radioisotopes; D008863:Microspheres; D008955:Models, Cardiovascular; D010104:Oxygen Radioisotopes; D011817:Rabbits; D011868:Radioisotopes; D012039:Regional Blood Flow; D012538:Scandium; D012709:Serum Albumin; D012983:Soft Tissue Neoplasms / Q000098:blood supply / Q000000981:diagnostic imaging*; D014055:Tomography, Emission-Computed
D005710:Gallium Radioisotopes; D010104:Oxygen Radioisotopes; D011868:Radioisotopes; D012709:Serum Albumin; D002245:Carbon Dioxide; D012538:Scandium