[Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg].
The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. subtilis chromosome. Using a collection of plasmids bearing various deletions extending in sacB we developed a technique of deletion mapping based on plasmid integration in the chromosome of B. subtilis. A generalization of this technique is discussed.
D001412:Bacillus subtilis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006602:Hexosyltransferases / Q000235:genetics*; D010641:Phenotype; D013395:Sucrose / Q000494:pharmacology; D014169:Transformation, Bacterial
D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase
Analyse génétique de sacB, gène de structure d'une enzyme secrétée, la lévane-saccharase de Bacillus subtilis Marburg.