Positive selection by "panning" of B lymphocytes using unfractionated anti-immunoglobulin antiserum.
A simple method allowing a positive selection of mouse B lymphocytes by "panning" has been achieved using rabbit anti-mouse immunoglobulin antisera without further purification. Plastic petri dishes were coated with the gamma-globulin fraction of normal mouse serum and then incubated with excess rabbit anti-mouse gamma globulin antiserum. These plates were then used for the selection of B lymphocytes by incubation of spleen cells in the plates. The adherent cells wee above 95% B lymphocytes as judged by immunofluorescence staining, proliferated in response to bacterial lipopolysaccharide and were not stimulated by concanavalin A.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007106:Immune Sera; D007163:Immunosorbent Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D003208:Concanavalin A