An enhanced chemiluminescence enzyme immunoassay for serum progesterone.
A competitive enhanced luminescent enzyme immunoassay for serum progesterone is described, which is based on a 11 alpha-hydroxyprogesterone 11-hemisuccinyl-horseradish peroxidase conjugate and a black polystyrene microtitre plate sensitised with anti-progesterone IgG. Bound label was determined using a mixture of 4-iodophenol, luminol and peroxide, and the light emitted from the wells of the plate quantitated using a luminescent plate reader. The assay was sensitive (detection limit 0.5 pg), precise (CV 2.7 - 9.0% in the concentration range 4.3-67.7 nM) and showed good correlation (r = 0.99) with a conventional radioimmunoassay.
D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D010544:Peroxidases; D011374:Progesterone / Q000097:blood*
D011374:Progesterone; D006735:Horseradish Peroxidase; D010544:Peroxidases