Persistent expression of Ia-antigen on a subpopulation of murine resident peritoneal macrophages.
The stability of Ia-antigen expression on murine resident peritoneal macrophages was assessed during the course of in vitro culture. Contrary to published findings with radioimmunoassays and immunofluorescence assays, the cultured cells bore Ia-antigen, as shown by their rosetting with sheep erythrocytes coupled with anti-Ia.2 monoclonal antibody. In support of this finding, cultured cells presented the copolymer of glutamine, alanine, and tyrosine (GAT) to GAT-primed T lymphocytes in an Ia-dependent manner. Thus, functional Ia antigen is present on cultured macrophages. Disappearance of the antigen after fixation of macrophages with either glutaraldehyde or paraformaldehyde, a routine procedure in the radioimmunoassays and immunofluorescence assays, explains its presumed absence on cultured cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001202:Ascitic Fluid / Q000166:cytology; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D005260:Female; D005976:Glutaral / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000276:immunology; D008264:Macrophages / Q000145:classification / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010455:Peptides / Q000276:immunology; D011108:Polymers; D011863:Radioimmunoassay; D011946:Receptors, Antigen / Q000187:drug effects; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D011108:Polymers; D011946:Receptors, Antigen; C020749:GAT; D005976:Glutaral