Susceptibility of ribosomes of the tetracycline-producing strain of Streptomyces aureofaciens to tetracyclines.
Ribosomes from cells of Streptomyces aureofaciens producing tetracycline antibiotics (Tc-ribosomes) differ in electrophoretic mobility of ribosomal proteins S2, S10 and L19 from those of the same strain, where the production of tetracyclines was suppressed by changed cultivation conditions (C-ribosomes). Purified tight vacant couples C- and Tc-ribosomes are equally active in the translation of poly(U). Both types of S. aureofaciens ribosomes are more sensitive to tetracycline and chlortetracycline than ribosomes of Escherichia coli in the Phe-tRNA binding and the translation of poly(U).
D004926:Escherichia coli / Q000187:drug effects; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000187:drug effects*; D013304:Streptomyces aureofaciens / Q000187:drug effects*; D013752:Tetracycline / Q000096:biosynthesis / Q000494:pharmacology*
D012346:RNA, Transfer, Amino Acyl; D012269:Ribosomal Proteins; D011072:Poly U; D010649:Phenylalanine; D013752:Tetracycline