The optimal conditions for microtiter cultures of human peripheral lymphocytes. I. Effects of bottom shapes of culture wells.
The influence of shapes of the bottom of culturing wells on lymphocyte proliferation was investigated. The lymphocytes were separated from the peripheral blood of eight donors by Ficoll-Hypaque density gradient centrifugation. Cells at 5 X 10(5)/ml were incubated in the absence, or in the presence, of PHA or Con A. The degrees of proliferation were compared in cultures using wells with variously shaped bottoms--flat, round and conical. The cultures in wells of flat bottoms provided the lowest background and highest mitogen-induced cell proliferation, whereas those in conical bottoms caused highest background and lowest mitogen-induced cell proliferation among the 3 types of bottom shapes. The peak proliferation in the presence of mitogen was at the end of the 3rd day in both round and conical bottoms, and at the end of the 4th day or later in flat bottoms.
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003584:Cytological Techniques; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013997:Time Factors
D010835:Phytohemagglutinins; D003208:Concanavalin A