The influence of ethionine on the phosphorylation state of adenine nucleotides in isolated hepatocytes.
The influence of D,L-ethionine (5 mM) was tested on hepatocytes isolated from fed or fasted rats. The digitonine fractionation procedure [6] was used to determine the repartition of adenine nucleotides and inorganic phosphate. Ethionine induces a decrease in the intracellular ATP content; this decrease is located both in the cytosol and in the mitochondria of fed and fasted rat hepatocytes. The observed effects of ethionine are: 1) fed rats: increase in glycolysis, decrease in mitochondrial ATP/ADP x PO4 and mitochondrial NAD+/NADH ratios; 2) fasted rats: decrease in neoglucogenesis from lactate + pyruvate or alanine, decrease in cytosolic ATP/ADP x PO4 and cytosolic NAD/NADH ratios.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D005001:Ethionine / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D010710:Phosphates; D005947:Glucose; D005001:Ethionine