Two modules from the hypersuppressive rho- mitochondrial DNA are required for plasmid replication in yeast.
In the yeast hypersuppressive (HS) rho- mutants most of the mitochondrial genome is deleted, but the remainder containing one of the three rep sequences is amplified. One of these sequences, rep2, and its flanking regions have been previously cloned and reported to promote autonomous plasmid replication in yeast. The present study suggests that the Ars activity associated with this HS rho- mitochondrial DNA (mtDNA) fragment is due to the presence in cis of at least two modules: (i) the 11-bp consensus sequence 5'-ATAAACTATAAAAT-3', common to several ars sequences, and (ii) a palindromic sequence of the mitochondrial replicator. Proper spacing between the two modules, which varies from about 100 to 200 bp, is required for the Ars+ activity.
D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005800:Genes, Fungal; D009154:Mutation; D010957:Plasmids; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
D004271:DNA, Fungal; D004272:DNA, Mitochondrial