The purification of myosin long subfragment 2 by DEAE-Sepharose CL-6B ion-exchange chromatography.
A new method for the purification of myosin long subfragment 2 is presented. This method is based on the fractionation, by DEAE-Sepharose CL-6B ion-exchange chromatography, of either chymotryptic hydrolysates of heavy meromyosin or tryptic hydrolysates of myosin total rod. Although emphasis is given to the purification of long subfragment 2, the method could be easily adapted to the purification of short subfragment 2.
D000818:Animals; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D009132:Muscles / Q000032:analysis; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification*; D011817:Rabbits; D012685:Sepharose / Q000031:analogs & derivatives
D015879:Myosin Subfragments; D010446:Peptide Fragments; C043982:DEAE-Sepharose CL-6B; D012685:Sepharose; D009218:Myosins