Teleocidin-induced phenotypic changes in thymic ALL cell line, HPB-ALL.
An indole alkaloid, teleocidin, induced phenotypic differentiation in the thymic ALL cell line HPB-ALL. Within 30 min of seeding in the presence of teleocidin, the cells formed a smooth round shape. Upon 5-day exposure to teleocidin, most of the cells became smaller and reminiscent of large or atypical lymphocytes. A thymic antigen stained with monoclonal antibody OKT6 was remarkably reduced while Leu2a-positive cells were increased in the treated cells. Upon teleocidin-induced phenotypic differentiation, the growth rate of cells was inhibited, their ability to incorporate DNA via [3H]-labelled precursors was reduced, their ability to bind sRBC rosettes was increased. These changes were paralleled to that induced by 12-o-tetradecanoyl-phorbol-13-acetate.
D000293:Adolescent; D000470:Alkaloids / Q000494:pharmacology*; D001667:Binding, Competitive; D002273:Carcinogens / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D008235:Lyngbya Toxins; D008297:Male; D010641:Phenotype; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism; D012397:Rosette Formation; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000470:Alkaloids; D002273:Carcinogens; D008235:Lyngbya Toxins; D010703:Phorbol Esters; C022176:teleocidins; D015240:Phorbol 12,13-Dibutyrate; D004247:DNA; D013755:Tetradecanoylphorbol Acetate