[The co-localization of neurohypophysis peptides and the corticotropin-releasing factor in the rat brain. Contribution of morphometric analyses].
Using adjacent serial brain sections, a morphometric method has been developed for analysing the coexistence of the neurophysial hormones, vasopressin (VP) and oxytocin (OT), with their specific neurophysins (N). A significative correlation was found between the immunoreactive areas stained with (1) anti-VP and anti-N-VP sera, and between the immunoreactive areas detected with anti-OT and anti-N-OT antibodies. Besides, the immunoreactive areas stained with (1) anti-VP and anti-OT antibodies, (2) anti-N-OT and anti-VP antibodies, (3) anti-N-OT and anti-N-VP antibodies or (4) anti-OT and anti-N-VP antibodies were totally independent. A different method projecting the microscope images on a reference grid with a camera lucida permitted to quantify the coexistence of an ovine corticotropin-releasing factor-related-peptide (41-CRF) with OT in the paraventricular neurons of the Brattloro rat brain. In these animals, the same method applied after total hypophysectomy demonstrated that the neurons synthezising simultaneously 41-CRF and OT projected their axons to the neurohypophysis; the same operation increased the relative number of neurons containing 41-CRF only; it can be supposed that they originated the infundibular terminals.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007031:Hypothalamus / Q000378:metabolism; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000378:metabolism; D009481:Neurophysins / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism; D010909:Pituitary Hormones, Posterior / Q000378:metabolism*; D051381:Rats; D011910:Rats, Brattleboro; D014667:Vasopressins / Q000378:metabolism
D009481:Neurophysins; D010909:Pituitary Hormones, Posterior; D014667:Vasopressins; D010121:Oxytocin; D003346:Corticotropin-Releasing Hormone
La colocalisation des peptides neurohypophysaires et de la corticolibérine dans le cerveau de rat. Apport des méthodes d'analyse d'images.