Purine-resistant Drosophila melanogaster result from mutations in the adenine phosphoribosyltransferase structural gene.
Mutants of Drosophila melanogaster selected for resistance to purine killing are deficient in adenine phosphoribosyltransferase (APRT; E.C. 2.4.2.7) activity. Genetic mapping and complementation analysis demonstrate that purine resistance, deficiency of APRT activity, and differences in the isoelectric point of APRT result from alterations at a single locus, Aprt (map position, 3:3.03). The level of APRT activity shows gene dose dependence in Aprt heterozygotes and in flies that are haploid for different Aprt alleles. Drosophila APRT is a dimer composed of apparently identical 23,000-dalton subunits. These results suggest that Aprt contains the structural gene for APRT.
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008970:Molecular Weight; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D011687:Purines / Q000633:toxicity*; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D011687:Purines; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase