Effect of age on Agaricus bisporus PHA-B stimulated insulin release and 45Ca2+ uptake in vitro by islets of Langerhans.
The in vitro basal insulin release and 45Ca2+ uptake by rat islets of Langerhans remain unaffected by the animal's age. However, when Agaricus bisporus lectin PHA-B is added to the medium, there is a significant stimulation of basal insulin secretion and 45Ca2+ uptake. The islets isolated from younger rats are more sensitive to A. bisporus PHA-B stimulation and register 1.5-fold and 3-fold more increases of insulin release and 45Ca2+ uptake respectively as compared to islets isolated from 12-month-old rats. Age-related conformational changes in the B-cell membrane are suggested as a possible explanation for the above observation.
D000375:Aging; D000818:Animals; D001487:Basidiomycota; D002118:Calcium / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051381:Rats
D007328:Insulin; D037102:Lectins; D002118:Calcium