Antinuclear antibody determinations in Ro(SSA)-positive, antinuclear antibody--negative lupus and Sjögren's syndrome patients.
Antinuclear antibody determinations were performed on the sera of twenty-eight Ro(SSA)-positive patients, who failed to demonstrate significant antinuclear antibody titers on mouse liver, with the use of various heterologous and homologous substrates. These studies demonstrated the following: (1) The existence of substrate specificity among various tissues and cells employed in the detection of antinuclear antibodies in Ro(SSA)-positive patients. (2) The existence of a significant group of Ro(SSA)-positive lupus patients with prominent cutaneous findings and systemic disease who, despite the employment of a variety of antinuclear substrates, failed to demonstrate significant antinuclear antibody titer.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D002460:Cell Line; D003593:Cytoplasm / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D008099:Liver / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051381:Rats; D012859:Sjogren's Syndrome / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; C035356:SS-A antibodies