Peripheral blood leukocyte adherence inhibition (LAI) reactivity before and during progressive growth of B77 tumor in rats.
Sequential changes in B77-rat fibrosarcoma (B77-RF) extract-induced leukocyte adherence inhibition (LAI) reactivity of peripheral blood leukocytes (PBL) were investigated in rats from the time of tumor inoculation through progressive growth and/or surgical excision of tumor. The time-course profiles of LAI reactivity of PBL in tumor bearing rats correlated with the stage of tumor growth. LAI reactivity of PBL in tumor bearing rats increased during 5-15 days after cell inoculation. Simultaneously, using in vitro 3H-uridine incorporation method for detection of T-cell population, the significantly increased number of immunocompetent lymphocytes was found. During progressive tumor growth (16 days and more after tumor cell inoculation) the LAI reactivity decreased. In this period moderate depletion of immunocompetent population of lymphocytes was observed. A significant decline in LAI reactivity was noted 10 days after a thorough excision of the tumors as compared to that prior to the surgical intervention. The kinetics of the observed time-course appearance of LAI reactivity and the demonstration of a rapid response following tumor challenge indicate further that LAI is a reliable in vitro measure of in vivo tumor immunity.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology* / Q000473:pathology; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D007957:Leukocyte Adherence Inhibition Test; D051381:Rats; D011919:Rats, Inbred Strains
D000951:Antigens, Neoplasm