The effect of alcohol stress on nicotinamide adenine dinucleotide (NAD+) levels in Drosophila.
Previous studies carried out in mammalian systems indicated that an organism's NAD+/NADH balance is carefully regulated but can be destabilized by dietary stresses. Since Drosophila alcohol dehydrogenase (ADH) uses NAD+ to remove a hydrogen from ethanol in the first step of alcohol catabolism, it is possible that under alcohol stress conditions the in vivo NAD+ levels in Drosophila may decrease. In this study genetically homozygous flies were stressed with maximally sublethal concentrations of ethanol (10%) for periods of up to 24 hr. The results indicate that NAD+ levels do in fact drop by at least 20% in response to ethanol stress. Evidence is presented that suggests that this decrease is the direct result of ADH-mediated catabolism.
D000433:1-Propanol / Q000494:pharmacology; D000222:Adaptation, Physiological; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism
D009243:NAD; D000431:Ethanol; D000433:1-Propanol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase