A novel immunoradiometric assay for human liver ferritin.
Rivanol, the cationic salt of an acridine base, has been used as a novel separation procedure in an immunoradiometric assay for human liver ferritin. The separation step is based on the differences in charge and molecular weight between the labelled antibody-ferritin complex and free labelled immunoglobulins. The resultant assay is simple, reproducible and sufficiently sensitive to determine serum concentrations of ferritin.
D000936:Antigen-Antibody Complex / Q000378:metabolism; D004975:Ethacridine / Q000031:analogs & derivatives; D005293:Ferritins / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D011863:Radioimmunoassay / Q000379:methods
D000936:Antigen-Antibody Complex; D005293:Ferritins; D004975:Ethacridine