Colony-stimulating activity production by hemopoietic organ fibroblastoid cells in vitro.
We have demonstrated that hemopoietic organ fibroblastoid cells can produce colony-stimulating activity (CSA) in vitro. Skin fibroblasts also produced CSA. The colonies which formed were restricted to the granulocyte/macrophage differentiation pathways. A linear relationship was obtained for granulocyte/macrophage colony formation in the presence of a variable number of bone marrow-derived fibroblastoid colonies. Fibroblastoid cells did not produce biologically detectable burst-promoting activity for erythroid progenitors. The importance of these observations with respect to in vitro hemopoietic regulatory phenomena are considered.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism*; D006098:Granulocytes / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007840:Latex; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008863:Microspheres; D010587:Phagocytosis; D012867:Skin / Q000166:cytology; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D003115:Colony-Stimulating Factors; D007840:Latex