Partial purification and some properties of a new papain inhibitor from psoriatic scales.
A new papain inhibitor was purified from psoriatic epidermal scales using gel chromatography and anion exchange chromatography. The purified protein inhibited papain and ficin but not cathepsin B, cathepsin H, trypsin, or chymotrypsin. Isoelectric focusing revealed 3 major inhibitor variants with pI's of 7.3, 6.9, and 6.5. A Mr of 38,000 was obtained by a gel chromatographic method for the crude inhibitor. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis the Mr values of the isoelectric variants were: 43,000 for the variant pI 7.3, 43,000 and 35,000 for the variant pI 6.9, and 34,000-35,000 for the variant pI 6.5. An antiserum of the inhibitor was used to locate the inhibitor in the psoriatic and normal epidermis. In psoriatic epidermis, the inhibitor was found in the peripheral cytoplasm of spinous cells and in the scale. In normal epidermis, the staining was seen only in orifices of hair follicles. An inhibitor with similar size and antigenic properties to that isolated from the psoriatic scales was demonstrated in extracts made from the whole-thickness epidermis but not in extracts from the healthy epidermal scales, the dermis, the liver, the spleen, or the blood serum.
D002102:Cadaver; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010206:Papain / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000032:analysis / Q000302:isolation & purification*; D011565:Psoriasis / Q000209:etiology / Q000378:metabolism*
D011480:Protease Inhibitors; D010206:Papain