Simple and rapid method for isolating large plasmid DNA from lactic streptococci.
A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.
D004269:DNA, Bacterial / Q000302:isolation & purification*; D013294:Lactococcus lactis / Q000235:genetics*; D008722:Methods; D010957:Plasmids
D004269:DNA, Bacterial