Isolation of highly purified Golgi membranes from rat liver. Use of cycloheximide in vivo to remove Golgi contents.
Following administration of cycloheximide to rats in order to deplete the liver of secretory products, Golgi membranes have been isolated largely free of internal contents. These membranes have a high specific activity of galactosyltransferase (400 times that of the homogenate) and are thought to be derived from the trans Golgi. Their phospholipid and polypeptide composition resembles that of Golgi membranes prepared by other procedures but their triacylglycerol and cholesterol contents are greatly reduced. These results conflict with previous reports that trans Golgi membranes are rich in cholesterol.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002784:Cholesterol / Q000378:metabolism; D003513:Cycloheximide; D005700:Galactosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D007425:Intracellular Membranes; D008563:Membrane Lipids / Q000032:analysis; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D000418:Albumins; D008563:Membrane Lipids; D014280:Triglycerides; D002784:Cholesterol; D003513:Cycloheximide; D005700:Galactosyltransferases