Micro-CO2-incubator for use on a microscope.
A simple micro-CO2-incubator designed for use on the stage of an inverted microscope is described. This micro-incubator is easy to use, offers a handy tool for the culture of cells under the microscope and its performance compares well with that of a conventional CO2-incubator. A standard disposable culture dish can be placed in the micro-incubator. The culture medium is covered by a gas-permeable layer of mineral oil, this protects the culture from the environment without affecting the culture conditions and allows easy cell manipulation under microscopical control.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D007185:Incubators; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008846:Micromanipulation / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation; D009994:Osmolar Concentration; D010313:Partial Pressure; D013696:Temperature
D002245:Carbon Dioxide