In vitro, non enzymatic labelling of histone H1 with [14C] acetyl CoA.
Histones H 1A and H 1B, in nuclear homogenates or after purification, are able to be acetylated or labelled by [14C] acetyl CoA in the absence of enzyme, as shown after blotting into nitrocellulose sheets. The reaction is pH-dependent, resistant to 2 M NaCl and does not occur at high ionic strength.
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D006657:Histones / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride; D013997:Time Factors
D002250:Carbon Radioisotopes; D006657:Histones; D012965:Sodium Chloride; D000105:Acetyl Coenzyme A