Rapid molecular weight estimation and separation of selected immunoglobulin chains by high speed gel filtration.
Rapid and reliable molecular weight estimations of reduced and alkylated immunoglobulin heavy or light chains were performed by high speed gel filtration in 6 M guanidinium chloride using a short (30 cm X 7.5 mm) TSK 3000 SW type column. Molecular weight estimations based on Kav values of eluted polypeptides and glycopolypeptides were generally unaffected by protein bound carbohydrate. Rapid separation of immunoglobulin H and L chains was also achieved during high speed gel filtration.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006020:Glycopeptides / Q000276:immunology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification*; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification*; D007142:Immunoglobulin gamma-Chains / Q000302:isolation & purification; D007145:Immunoglobulin kappa-Chains / Q000302:isolation & purification; D007146:Immunoglobulin lambda-Chains / Q000302:isolation & purification; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology
D006020:Glycopeptides; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D010455:Peptides