Applications of fast protein liquid chromatography TM in the separation of plasma proteins in urine and cerebrospinal fluid.
Fast Protein Liquid Chromatography TM (FPLC), in which an anion-exchange column is used, provides rapid separation and reproducible profiling of the plasma proteins in urine and cerebrospinal fluid (CSF). Chromatographic separation of the proteins takes 1 h for urine specimens and 45 min for CSF. The elution sequence from the anion-exchange column is similar to the electrophoretic mobility. Individual proteins have the same retention times independently of which type of specimen is used. The elution characteristics of 21 plasma proteins have been identified. We illustrated some applications of this system, including the profiling of tubular protein-uria, the isolation of Bence Jones proteins from urine, and the investigation of hemoglobin-derived products in the CSF.
D000328:Adult; D001798:Blood Proteins / Q000134:cerebrospinal fluid / Q000302:isolation & purification* / Q000652:urine; D002021:Buffers; D002648:Child; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification; D007674:Kidney Diseases / Q000652:urine; D007678:Kidney Glomerulus; D007684:Kidney Tubules; D007938:Leukemia / Q000652:urine; D011507:Proteinuria / Q000652:urine
D001798:Blood Proteins; D002021:Buffers; D007147:Immunoglobulin Light Chains