Errors in DNA synthesis induced by aflatoxin B1 modification of poly(dC-dG).
Aflatoxin B1 reacts with poly(dC-dG) to form guanine adducts and apurinic sites. Such modification strongly inhibits the template capacity of the polynucleotide and leads to an increase in non-complementary nucleotide incorporation. The template inhibition and induced errors are reduced by mild heat depurination which removes all 7-substituted modifications.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D011089:Polydeoxyribonucleotides
D000348:Aflatoxins; D002273:Carcinogens; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D016604:Aflatoxin B1