Identification of phospholipid platelet-activating factor (1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) in human amniotic fluid and urine.
Human amniotic fluid and fetal urine were examined for the presence of phospholipid platelet-activating factor (PAF). PAF was detected in lipid extracts of some samples of amniotic fluid obtained from women in labor but it was undetectable in samples of amniotic fluid obtained before the onset of labor. PAF was identified by chromatographic mobility, platelet aggregation and chemical modifications. LysoPAF was also present in amniotic fluid at higher concentrations than those of PAF. Both PAF and lysoPAF were identified also in newborn and adult urine.
D000653:Amniotic Fluid / Q000032:analysis*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000032:analysis* / Q000494:pharmacology / Q000652:urine; D010974:Platelet Aggregation / Q000187:drug effects; D011247:Pregnancy
D001095:Arachidonic Acids; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D002118:Calcium; D007213:Indomethacin