UDP-glucuronosyltransferase activity and bilirubin conjugation in the bullfrog.
Bile pigments of bile and serum of Rana catesbeiana were investigated by means of high-pressure liquid chromatography. The major pigment in both bile and serum was bilirubin IX alpha. Bilirubin UDP-glucuronosyltransferase activity was found in the livers of all animals examined, but no conjugated bilirubin was detectable in the bile. Frog bile was found to contain large amounts of beta-glucuronidase. When the beta-glucuronidase inhibitor saccharo-1,4-lactone was introduced into the gall bladder followed by an exogenous bilirubin load, bilirubin glucuronide appeared in the bile.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001654:Bile Pigments / Q000097:blood / Q000378:metabolism; D001663:Bilirubin / Q000031:analogs & derivatives / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011892:Rana catesbeiana
D001654:Bile Pigments; C016266:bilirubin glucuronate; D014453:Glucuronosyltransferase; D001663:Bilirubin