[Incorporation of methionine into a peptic partial hydrolyzate from faba bean protein isolate by plastein reaction with thermitase].
The contents of free methionine methyl esters and methionine as well as the bound quantities of methionine were determined in order to evaluate the effectiveness of the covalent incorporation of methionine into peptic partial hydrolyzate in the course of the plastein reaction with addition of L-, DL- or D-methionine ethyl esters. The plastein reaction was performed with thermitase as reenzyme. The methionine incorporation was found to take place stereospecifically. The incorporation degree of the methionine ester is influenced by a thermitase-catalyzed and a non-enzymatic ester saponification.
D010450:Endopeptidases / Q000378:metabolism*; D007887:Fabaceae / Q000032:analysis; D008715:Methionine / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D010946:Plants, Medicinal; D011492:Protein Hydrolysates / Q000032:analysis*; D012697:Serine Endopeptidases; D013237:Stereoisomerism
D010940:Plant Proteins; D011492:Protein Hydrolysates; C011531:plastein; D008715:Methionine; D010450:Endopeptidases; D012697:Serine Endopeptidases; C022728:thermitase
Untersuchungen zum Methionineinbau in ein peptisches Partialhydrolysat aus Ackerbohnenproteinisolat durch Plasteinreaktion mit Thermitase.