[Fixation of antibodies by covalent bonds to polystyrene: use of the immunoenzyme technic for the detection of Escherichia coli K99 in calf feces].
A solid phase enzyme immunoassay using antibodies covalently bound to polystyrene balls is developed. Results show the same sensitivity as the method with antibodies adsorbed to solid support. By means of K99+ E. coli detection on diarrhoeic calves, results are compared with bacteriological findings. The interest of the method is discussed.
D000818:Animals; D000914:Antibodies, Viral; D000954:Antigens, Surface / Q000032:analysis*; D001427:Bacterial Toxins; D002417:Cattle; D004926:Escherichia coli / Q000276:immunology*; D005243:Feces / Q000276:immunology*; D007124:Immunoenzyme Techniques; D011137:Polystyrenes
D000914:Antibodies, Viral; D000954:Antigens, Surface; D001427:Bacterial Toxins; C031429:K99 antigen; D011137:Polystyrenes
Fixation d'anticorps par liaisons covalentes sur le polystyrène: application de la technique immunoenzymatique à la recherche de Escherichia coli k99+ dans les fèces de veaux.