Citric acid airway constriction in dogs with hyperreactive airways.
To understand the mechanisms underlying the bronchoconstrictor response to 10% citric acid administered for 5 min in Basenji-Greyhound (BG) dogs, we evaluated the protection afforded by atropine (0.2 and 0.4 mg/kg iv) and by aerosols of isoproterenol (1 mg/ml) and cromolyn sodium (20 mg/ml). In untreated dogs, citric acid increased pulmonary resistance by 4.6- to 11.5-fold and decreased dynamic compliance (Cdyn) to 45-55% of the control response. Isoproterenol and cromolyn sodium significantly reduced the response, whereas atropine did not. Moreover we have demonstrated in the arterial plasma of these dogs a slow-reacting substance (SRS) after, but not before, citric acid challenge. This SRS exhibits both pharmacologic properties and chemical characteristics similar to leukotrienes. We conclude that mediators of immediate-type hypersensitivity rather than reflex mechanisms play a dominant role in the production of airway constriction during citric acid (5-min) challenge in BG dogs.
D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D001285:Atropine / Q000627:therapeutic use; D001985:Bronchial Provocation Tests; D001986:Bronchial Spasm / Q000139:chemically induced* / Q000150:complications / Q000517:prevention & control; D002951:Citrates; D019343:Citric Acid; D004205:Cromolyn Sodium / Q000627:therapeutic use; D004285:Dogs; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000150:complications*; D007545:Isoproterenol / Q000627:therapeutic use; D008170:Lung Compliance / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D012140:Respiratory Tract Diseases / Q000150:complications*; D013189:SRS-A / Q000037:antagonists & inhibitors / Q000494:pharmacology
D002951:Citrates; D013189:SRS-A; D019343:Citric Acid; D001285:Atropine; D007545:Isoproterenol; D004205:Cromolyn Sodium