Turbidimetric kinetic method for serum low density lipoprotein quantitation.
A turbidimetric kinetic fixed-time method for the rapid determination of serum low density lipoprotein (LDL) has been developed. It is based on specifically precipitating this lipoprotein with the use of a combination of heparin, Ca2+, EDTA and lipase. The method has been adapted to the Eppendorf spectrum line photometer and to the ENI Gemsaec centrifugal analyzer. It yielded satisfactory results with regard to linearity and precision. The values agreed closely to those obtained by conventional procedures for LDL-cholesterol and LDL-apolipoprotein B. The predictive value of this new method for the assessment of the individual risk of vascular disease remains to be proven by clinical and epidemiological studies.
D002118:Calcium; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL; D004492:Edetic Acid; D006493:Heparin; D006801:Humans; D007700:Kinetics; D008049:Lipase; D008077:Lipoproteins, LDL / Q000097:blood*; D009391:Nephelometry and Turbidimetry / Q000379:methods*
D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D006493:Heparin; D002784:Cholesterol; D004492:Edetic Acid; D008049:Lipase; D002118:Calcium