Deficient cytidine monophospho-N-acetylneuraminic acid: glycoprotein sialyltransferase activity in a clone of KB cells with altered cell fusion ability.
Lines of KB cells resistant to Sendai virus-induced cytolysis have been isolated and characterized (Toyama, S., Toyama, Su., and Uetake, H. (1977) Virology 76, 503-515). This study is concerned with the nature of this mutation. Plasma membrane fractions from Sil cells were found to have decreased amount of sialic acid and the same amount of galactose as compared to the membranes from parental KB cells. Sil cells exhibited an increase in sensitivity to toxic effects of ricin and a decrease in sensitivity to wheat germ agglutinin. Binding of wheat germ agglutinin to Sil cells was markedly decreased. Several membrane glycoproteins of Sil cells migrated slightly faster than the corresponding bands of wild type membrane when examined by gel electrophoresis in sodium dodecyl sulfate. Sil cells had decreased sialyltransferase activity that catalyzed the transfer of sialic acid residues from CMP-N-acetylneuraminic acid to glycoprotein acceptors containing Gal beta 1 leads to 3GalNAc alpha 1 leads to O-Ser(Thr) chain. The decreased enzyme activity could not be accounted for by the presence of inhibitors, altered pH optimum, or increased sialidase or CMP-sialic acid hydrolase activities. These results indicate that a molecular basis for the Sil cell phenotype might be the deficiency of sialyltransferase.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002277:Carcinoma / Q000201:enzymology*; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005700:Galactosyltransferases / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D037102:Lectins / Q000378:metabolism; D009062:Mouth Neoplasms / Q000201:enzymology*; D009374:Neoplasms, Experimental / Q000201:enzymology*; D012799:Sialyltransferases / Q000172:deficiency*; D014166:Transferases / Q000172:deficiency*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D014166:Transferases; D005700:Galactosyltransferases; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase