Direct immunofluorescence for ABH blood group isoantigens: use of FITC-conjugated lectins.
A direct immunofluorescence method was used to detect A, B, or H blood group isoantigens in deparaffinized tissue sections of 30 bladder tumors of various grades and stages. The results were compared with those of the specific red cell adherence test performed on the replicate sections. The two methods showed identical results for isoantigens A, B, and H. Direct immunofluoresence method appeared to be less time-consuming, more simple to perform, and reproducible with less subjectivity in the interpretation of the results.
D000017:ABO Blood-Group System / Q000276:immunology*; D002448:Cell Adhesion; D004912:Erythrocytes / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D037102:Lectins / Q000032:analysis*; D013861:Thiocyanates; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis
D000017:ABO Blood-Group System; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007519:Isoantigens; D037102:Lectins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate