An antigen shared by human granulocytes, monocytes, marrow granulocyte precursors and leukemic blasts.
An antibody to human granulocytes was raised in rabbits by immunization with granulocytes pretreated with rabbit antibody to contaminating antigens. The antibody reacted not only with granulocytes but also with monocytes and bone marrow granulocyte precursors including colony-forming units in culture (CFU-C). In tests with leukemic cells, the antibody reacted with blasts from most (8 of 9) patients with acute myelomonoblastic leukemia and from some patients with acute myeloblastic leukemia, morphologically undifferentiated acute leukemia and chronic myelogenous leukemia in blast crisis. The antibody did not react with blasts from patients with acute lymphoblastic leukemia nor with leukemic cells from patients with chronic lymphocytic leukemia.
D000818:Animals; D001345:Autoradiography; D001853:Bone Marrow / Q000276:immunology*; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007938:Leukemia / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera