Cell culture quality control by rapid isoenzymatic characterization.
Procedures that involve cell cultures require careful quality control to avoid inter- and intraspecies contamination. We have developed an electrophoresis technique that can be used routinely in cell culture laboratories to monitor cell line integrity. The method involves the isoenzymatic separation of nine polymorphic enzymes, three of which can be used for cell line species determinations and seven of which can be used for human cell line characterizations. Examples of how the system has been applied to both inter- and intraspecies identifications are described. The routine application of this protocol would be a valuable asset for laboratories concerned with establishing effective cell culture quality control.
D000818:Animals; D002415:Cats; D002460:Cell Line; D002645:Chickens; D003584:Cytological Techniques; D004587:Electrophoresis, Agar Gel; D000882:Haplorhini; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D010641:Phenotype; D011786:Quality Control; D011817:Rabbits; D051381:Rats
D007527:Isoenzymes