Enzyme immunoassay for serum 18-hydroxycorticosterone and its clinical application.
An enzyme immunoassay for serum 18-hydroxycorticosterone was established using alkaline phosphatase as a label. The antiserum for 18-hydroxycorticosterone was produced by immunization of rabbits with 18-hydroxycorticosterone 3-(O-carboxymethyl)oxime conjugated to bovine serum albumin. Sephadex LH-20 column chromatography was used to separate 18-hydroxycorticosterone from other steroids in serum samples. The minimal detectable amount of 18-hydroxycorticosterone was 50 pg/tube and the measurable range was from 5 to 1000 ng/dl when a 1.0 ml serum sample was used. Intra- and inter-assay coefficients of variance were 5.0% (n = 6) and 5.8% (n = 6), respectively. Four of 5 patients with aldosterone-producing adenoma had above-normal serum 18-hydroxycorticosterone levels.
D015069:18-Hydroxycorticosterone / Q000097:blood*; D000182:ACTH Syndrome, Ectopic / Q000097:blood; D000224:Addison Disease / Q000097:blood; D000236:Adenoma / Q000097:blood / Q000378:metabolism; D000312:Adrenal Hyperplasia, Congenital; D000450:Aldosterone / Q000378:metabolism; D003345:Corticosterone / Q000031:analogs & derivatives*; D003480:Cushing Syndrome / Q000097:blood; D006801:Humans; D006929:Hyperaldosteronism / Q000097:blood; D007124:Immunoenzyme Techniques
D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D003345:Corticosterone