Investigation into the possible genetic effects of diagnostic ultrasound.
The mutagenic effect of DUS (diagnostic ultrasound) was examined using two generators emitting continuous waves and pulsed waves, respectively. Three different test systems for mutagenic activity were employed in this study. 1. The frequency of sister chromatid exchanges (SCEs) was determined in metaphase chromosomes of human lymphocytes and of Chinese hamster ovary (CHO) cells sonicated at different stages of the cell cycle. 2. The induction of DNA single strand breaks was tested in CHO cells treated with continuous wave ultrasound. Following sonication an endonuclease was introduced into the cells converting single strand breaks to chromosomal aberrations scorable in the following metaphase. 3. The influence of DUS on the number of point mutations was evaluated in the Ames test. A tester strain of Salmonella typhimurium indicating base substitutions was sonicated. At the present stage, all results proved to be completely negative and could thus not support the view of any mutagenic activity of diagnostic ultrasound.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004277:DNA, Single-Stranded; D005260:Female; D006801:Humans; D008214:Lymphocytes; D009152:Mutagenicity Tests; D009154:Mutation; D010053:Ovary; D012486:Salmonella typhimurium / Q000235:genetics; D012854:Sister Chromatid Exchange; D014463:Ultrasonography / Q000009:adverse effects*
D004277:DNA, Single-Stranded