A comparison of two methods of labelling autologous platelets with 111In-oxine in five different species.
Several different methods for labelling autologous platelets with 111In-oxine have been described. However, no comparative study has been reported. In the present investigation two different labelling methods were compared in terms of labelling efficiency and platelet function in five species: human, dog, pig, rabbit and rat. One of the labelling methods, utilising among other things a serum albumin gradient separation of platelets and incubation of 111In-oxine in a water bath at 37 degrees C, was superior in all species with significantly higher labelling efficiency and unchanged platelet function.
D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D002499:Centrifugation, Density Gradient / Q000379:methods; D004285:Dogs; D006801:Humans; D006912:Hydroxyquinolines; D007204:Indium; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D010974:Platelet Aggregation; D011817:Rabbits; D011868:Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
D006912:Hydroxyquinolines; D009942:Organometallic Compounds; D011868:Radioisotopes; D007204:Indium; C032950:indium oxine; D015125:Oxyquinoline