Correlation of amino acid fucoside labeling patterns with tumorigenicity of mouse mammary epithelial cells.
The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosylthreonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to glucosylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer; D004848:Epithelium / Q000032:analysis; D005260:Female; D005643:Fucose / Q000031:analogs & derivatives* / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D008321:Mammary Glands, Animal / Q000032:analysis*; D008325:Mammary Neoplasms, Experimental / Q000032:analysis* / Q000139:chemically induced; D051379:Mice; D008807:Mice, Inbred BALB C
D000596:Amino Acids; D006027:Glycosides; D005643:Fucose; D015127:9,10-Dimethyl-1,2-benzanthracene