Reduction of lipofuscin by centrophenoxine and chlorpromazine in the neurons of rat cerebral hemisphere in primary culture.
Cells from neonatal rat cerebral hemispheres were dispersed by trypsin and cultured for 32 days. Histochemical, fluorescence, and electron microscopic analyses demonstrated that lipofuscin pigments increased in neuronal and non-neuronal cells in primary culture according to the lapse of time. When centrophenoxine (10(-4) or 5 X 10(-4) M) or chlorpromazine (10(-6) or 10(-5) M) was added to the medium, the accumulation of lipofuscin pigments in neurons was significantly reduced. However, the effects of these agents were not detected in non-neuronal cells.
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D006016:Glycolates / Q000494:pharmacology*; D008062:Lipofuscin / Q000378:metabolism*; D002504:Meclofenoxate / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects; D010860:Pigments, Biological / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D006016:Glycolates; D008062:Lipofuscin; D010860:Pigments, Biological; D002504:Meclofenoxate; D002746:Chlorpromazine