Apolipoprotein A-I isoforms in human lymph: effect of fat absorption.
The effect of fat feeding (100 g of cream) on the apoA-I isoproteins distribution has been analyzed by two-dimensional gel electrophoresis in the chylomicrons, VLDL, LDL, and HDL isolated from the thoracic duct lymph of patients undergoing lymph drainage for immunosuppression, Isoforms apoA-I3 and apoA-I4 are the most abundant apoA-I isoproteins in plasma lipoproteins as well as in lymph lipoproteins collected in the fasting state. Fat feeding, on the other hand, results in a marked change in the apoA-I isoform pattern in lymph chylomicrons and VLDL, with a significant increase in the relative concentration of the apoA-I1 isoform. As a result the total concentration of this isoprotein in the lymph increased. The data indicate that fat feeding is associated with major changes in the distribution of the apoA-I isoforms in the lymph (d less than 1.006 g/ml lipoproteins), which may be of significance in their plasma catabolism.
D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000302:isolation & purification* / Q000378:metabolism; D002784:Cholesterol / Q000302:isolation & purification; D004041:Dietary Fats / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007408:Intestinal Absorption; D008075:Lipoproteins, HDL / Q000302:isolation & purification*; D008196:Lymph / Q000502:physiology*; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000302:isolation & purification
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D004041:Dietary Fats; D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol