Improved T cell subset monitoring by immunogold staining following renal transplantation.
Enumeration of peripheral blood T cells, functional T cell subsets, and B cells by immunogold staining (IGS) was compared with analysis by flow cytometry in 52 renal allograft recipients. Results by the IGS method compared favorably with those obtained by flow analysis. IGS is a simple, inexpensive technique that is readily adaptable to the setting of posttransplant immune monitoring.
D000954:Antigens, Surface / Q000032:analysis; D005434:Flow Cytometry; D006046:Gold; D006801:Humans; D007158:Immunologic Techniques; D016030:Kidney Transplantation; D007958:Leukocyte Count; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000954:Antigens, Surface; D006046:Gold