[Production of heat-labile Escherichia coli enterotoxin in a synthetic medium and its assay on a culture of Vero cells].
A modification of the technique described by Evans for the production of Escherichia coli heat-labile enterotoxin was employed. Instead of Casamino yeast extract medium, which is toxic for Vero cells, Eagle's minimal essential medium (MEM) commonly used in cell cultures was substituted and the LT toxin produced in MEM was assayed on Vero cells.
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001681:Biological Assay; D002460:Cell Line; D002522:Chlorocebus aethiops; D003470:Culture Media; D004768:Enterotoxins / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D029968:Escherichia coli Proteins; D011112:Polymyxin B / Q000494:pharmacology
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli; D011112:Polymyxin B
Production de l'entérotoxine thermolabile de Escherichia coli sur milieu synthétique en vue de son dosage sur culture de cellules Vero.