Preferential binding of high molecular weight forms of von Willebrand factor to fibrillar collagen.
The time- and concentration-dependent binding of von Willebrand factor to fibrillar collagen was examined by following the disappearance from plasma of ristocetin cofactor activity and factor VIII-related antigen, the functional and immunologic determinants of von Willebrand factor. Examination of both bound and unbound factor VIII-related antigen by crossed immunoelectrophoresis revealed a preferential binding of the higher molecular weight forms of von Willebrand factor to fibrillar collagen.
D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D005169:Factor VIII / Q000378:metabolism; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats; D014841:von Willebrand Factor / Q000378:metabolism*
D001779:Blood Coagulation Factors; D014841:von Willebrand Factor; D005169:Factor VIII; D003094:Collagen