Detection of proteins which recognize and bind oriC sequences.
Extracts from E. coli capable of supporting in vitro oriC-dependent DNA replication have been examined with a protein blotting protocol to identify DNA-binding proteins. Four polypeptide chains with apparent affinity for oriC DNA were detected.
D002876:Chromosomes, Bacterial / Q000502:physiology*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D008970:Molecular Weight; D010957:Plasmids
D004268:DNA-Binding Proteins