Localization of DNA sequences promoting RNA polymerase I activity in Drosophila.
We used BAL-31 nuclease to delete sequences that surround the transcription initiation site of Drosophila ribosomal DNA. A series of deletions was used as templates for in vitro transcription in a Drosophila cell-free system to identify sequences that influence the activity of RNA polymerase I. Sequences that lie upstream of the site of transcription initiation (nucleotide + 1) affect ribosomal RNA synthesis. We show that the major promoter of polymerase I involves the sequence -43 to -27 and that the region between nucleotides -18 and +20 contains sequences capable of sustaining a low level of accurate transcription.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004275:DNA, Ribosomal; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D012318:RNA Polymerase I / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I