Automated rate-immunonephelometric determination of serum prealbumin (transthyretin).
We describe an automated determination of serum prealbumin (transthyretin), based on the maximum rate of light scatter of an antigen-antibody precipitate. Optimal dilutions of antibody reagent and of 100-microL serum samples are given. Within- and between-assay CVs are less than 5%. Results by this method are nearly identical with those obtained by a radial-immunodiffusion technique. Measuring the rate of light scatter provides a considerably faster test than equilibrium scatter, electrophoretic, or radial-immunodiffusion methods. Treatment before analysis to clear lipemic sera obviates falsely high results.
D000368:Aged; D000936:Antigen-Antibody Complex / Q000032:analysis; D001322:Autoanalysis / Q000379:methods; D004586:Electrophoresis; D006801:Humans; D005779:Immunodiffusion; D007158:Immunologic Techniques; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011228:Prealbumin / Q000032:analysis* / Q000276:immunology; D012709:Serum Albumin / Q000032:analysis*
D000936:Antigen-Antibody Complex; D011228:Prealbumin; D012709:Serum Albumin