Influence of various mitogens on the yield of sister-chromatid exchanges, induced by chemicals, in human lymphocytes.
The fluorochrome-plus-Giemsa (FPG) method of Perry and Wolff was used to compare the frequencies of sister-chromatid exchanges (SCEs) induced by cyclophosphamide (CP) or mitomycin C (MMC) in human lymphocytes stimulated by phytohaemagglutinin (PHA), concanavalin A (Con A), Wistaria floribunda (WFA), or lentil lectin (LcH-A) extracts. These 4 mitogens, differing in lectin valency and/or sugar specificity, are considered as activating primarily thymus-derived (T) lymphocytes. Regardless of the mitogen used, control cultures displayed a mean yield of about 8 SCEs/cell. A contact, of 1 h, with mitomycin alone or with cyclophosphamide and enzymatic activation, resulted in a significant augmentation of SCEs dependent on the mitogen used. An approximately 2-fold, 4-fold, or 6-fold increase in SCEs was observed for the cultures stimulated by PHA, Con A, and WFA or LcH-A respectively. Furthermore, there were mitogen-dependent differences in mitotic indices and cell-cycle kinetics in human lymphocytes harvested 72 h after stimulation.
D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003520:Cyclophosphamide / Q000633:toxicity*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008934:Mitogens / Q000494:pharmacology*; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D008940:Mitotic Index; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; D008934:Mitogens; D008937:Mitomycins; D009153:Mutagens; D016685:Mitomycin; D003520:Cyclophosphamide