Rapid assay for detection of microorganisms producing DNA-damaging metabolites.
Microorganisms producing DNA-damaging metabolites (i.e., fungi and streptomycetes) were detected by the Bacillus subtilis rec assay with agar plugs from plates on which the microorganisms had been grown. This assay allowed rapid identification of aflatoxinogenic fungi and streptomycetes producing strong DNA-damaging metabolites. For screening programs, several media have to be used to grow the microorganisms to be tested.
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics; D001681:Biological Assay / Q000379:methods*; D004269:DNA, Bacterial; D005658:Fungi / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis* / Q000494:pharmacology; D009183:Mycotoxins / Q000032:analysis* / Q000494:pharmacology; D011995:Recombination, Genetic; D013302:Streptomyces / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009153:Mutagens; D009183:Mycotoxins