A solid phase radioimmunoassay for detection of antibodies to extractable nuclear antigens.
A solid phase radioimmunoassay is described for the detection of autoantibodies to the saline-soluble extractable nuclear antigens, ribonucleoprotein (RNP) and SS-B (or La). This assay depends on enrichment of antigens from a crude, commercially available (Pel Freez, U.S.A.) extract of rabbit thymus by absorption to the F(ab)2 fraction of specific high titre antibody attached to a microtitre plate. Serum antibody reactive with this antigen is then detected by 125I-labelled Protein A. The assay is simple and is more sensitive than the gel diffusion assays in general use for detecting such antibodies.
D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001323:Autoantibodies; D001324:Autoantigens; D001327:Autoimmune Diseases / Q000276:immunology*; D002467:Cell Nucleus / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009698:Nucleoproteins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D012261:Ribonucleoproteins / Q000032:analysis*; D012859:Sjogren's Syndrome / Q000276:immunology; D000097585:SS-B Antigen
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen