[Genome mutation frequency in the cells of spleen colonies of different origins in NIH mice].
Genome mutation frequencies (GMF) were determined in cells of endogenous (from bone marrow) and exogenous (from bone marrow, spleen and embryonic liver) spleen colonies on the basis of variations in DNA contents of interphase nuclei. In cells of the former GMF varied from 1.1 X 10(-2) to 10.8 X 10(-2), and in the latter these were equal to 8.9 X 10(-2). In the cells of exogenous colonies derived from X-irradiated precursors (1.8 and 5.9 Gy) GMF were 10.1 X 10(-2) and 11.9 X 10(-2), resp. The mode of transplantation influenced greatly on the GMF: after an additional short transplantation (4-6 days) the number of GMF increased by 1.5-2 times. It is concluded that the increased number of GMF may be responsible for the limited life-span of bone marrow stem cells in the course of their serial transplantations in the irradiated syngenic mice.
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects / Q000648:ultrastructure; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004247:DNA / Q000032:analysis / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005787:Gene Frequency / Q000528:radiation effects; D007399:Interphase / Q000528:radiation effects; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D009154:Mutation; D013154:Spleen / Q000528:radiation effects / Q000637:transplantation / Q000648:ultrastructure*; D013997:Time Factors
D004247:DNA
Chastota genomnykh mutatsiĭ v kletkakh selezenochnykh koloniĭ razlichnogo proiskhozhdeniia u mysheĭ NIH.