Preparation of aflatoxin B1-BSA conjugate with high hapten/carrier molar ratio.
We reported previously on the properties of an anti-aflatoxin B1 (AFB) antiserum raised with a conjugate in which AFB is coupled at the C8 position to bovine serum albumin (BSA). The hapten/carrier protein molar ratio of the conjugate was 14. We have since been able to optimize the coupling conditions, resulting in the preparation of another conjugate, with a molar ratio of 45. Comparison of the 2 conjugates by double-gel immunodiffusion analysis shows that little BSA 'activity' remains on the conjugate with a high molar ratio; on the other hand, its capacity to precipitate in the presence of anti-AFB antibodies is increased.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000937:Antigen-Antibody Reactions; D002273:Carcinogens / Q000032:analysis; D002352:Carrier Proteins / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D007158:Immunologic Techniques; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism*
D000348:Aflatoxins; D002273:Carcinogens; D002352:Carrier Proteins; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D016604:Aflatoxin B1