Lipopolysaccharides of the cyanobacterium Microcystis aeruginosa.
Lipopolysaccharides (LPS) of two isolates of Microcystis aeruginosa were extracted with phenol/water and purified. Cesium chloride gradient ultracentrifugation of these preparations yielded only one fraction. The LPS contained significant amounts of 3-deoxy-D-manno-octulosonic acid, glucose, 3-deoxy sugars, glucosamine, fatty acids, fatty acid esters, hexoses, and phosphate. Heptose, a characteristic sugar component of the polysaccharide moiety of LPS of most gram-negative bacteria was absent. Lipopolysaccharides and lipid A hydrolysate of LPS preparations were active in mouse lethality and Limulus lysate gelation. The lipid A moiety was slightly less active in toxicity and Limulus lysate gelation assays than the intact LPS. The LPS and lipid A moiety of the two isolates of M. aeruginosa were less active in toxicity in mice and Limulus test than LPS of Salmonella abortus equi.
D000818:Animals; D002500:Centrifugation, Isopycnic; D055598:Chemical Phenomena; D002621:Chemistry; D004731:Endotoxins; D008033:Limulus Test; D008050:Lipid A / Q000302:isolation & purification / Q000633:toxicity; D008070:Lipopolysaccharides / Q000302:isolation & purification* / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046931:Microcystis / Q000032:analysis*
D004731:Endotoxins; D008050:Lipid A; D008070:Lipopolysaccharides