Activity of glycogen metabolizing enzymes in glucose deprived HT 29 adenocarcinoma cell-line.
When deprived of glucose, the cultured HT 29 adenocarcinoma cells are able to mobilize their glycogen within 4 hours. Glycogen phosphorylase is strongly activated during the first hour of glucose starvation. Then, while the a/a + b ratio for phosphorylase is declining, glycogen synthase is partially converted into the a form; this conversion does occur although glycogen phosphorylase is far from being totally inactivated. After 4 hours, activity of both a and total forms of glycogen synthase decrease. Cell UDP-glucose and glucose-6-P levels are declining during the 24 hours period of glucose starvation. Cell ATP content decreases by only 50 percent over the same period of time.
D000230:Adenocarcinoma / Q000201:enzymology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D005947:Glucose / Q000502:physiology*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis / Q000378:metabolism*; D006006:Glycogen Synthase / Q000502:physiology; D006801:Humans; D009374:Neoplasms, Experimental / Q000201:enzymology; D006005:Phosphorylases / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate; D006003:Glycogen; D006005:Phosphorylases; D006006:Glycogen Synthase; D005947:Glucose; D014532:Uridine Diphosphate Glucose