Uses and applications of reactive dyes in histopathologic technique: a new panchromic hematoxylin-Tanisol red stain for histologic sections and cytologic smears.
A new class of dyestuffs--Tanisol-reactive dyes--is introduced in histopathologic technique as eosin substitutes and/or complementary stains of the classical HE. In the hematoxylin-Tanisol stain a histochemical reaction takes place between the dye and the tissue in situ with the formation of strong dye-tissue covalent crosslinks. This provides a high fastness to wet treatments and a light fastness not hitherto achieved with eosin. The procedure is simpler to perform than that of the classical HE. Tanisol-combined techniques with PAS-Alcian blue 8GX, pH 2.5 and Alcian blue critical electrolytic concentration at different molarities, as well as with Hale's colloidal iron are also introduced. The stain has also proved very useful in the evaluation of cytologic smears.
D004396:Coloring Agents; D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D006652:Histological Techniques; D006801:Humans
D004396:Coloring Agents