Rat histamine N-methyltransferase. Quantification, tissue distribution, purification, and immunologic properties.
Histamine N-methyltransferase is a major enzyme for inactivating histamine in mammalian tissues. Development of a sensitive and specific assay for histamine N-methyltransferase has permitted quantification of this activity in 25 rat tissues. Additionally, renal histamine N-methyltransferase was purified to electrophoretic homogeneity. The pivotal step in the purification scheme was an affinity column procedure which involved the specific elution of histamine N-methyltransferase from DEAE-Sephacel by 1 mM histamine. The purified enzyme has a molecular weight of 33,400 and a narrow pH dependency with an optimum at 8.0-8.25. Specific antibody produced to renal histamine N-methyltransferase also immunoprecipitated the brain enzyme completely, therefore indicating neuronal and non-neuronal histamine N-methyltransferase share common antigenic determinants.
D000818:Animals; D000936:Antigen-Antibody Complex; D001921:Brain / Q000201:enzymology*; D006637:Histamine N-Methyltransferase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D007106:Immune Sera; D005779:Immunodiffusion; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008780:Methyltransferases / Q000378:metabolism*; D008970:Molecular Weight; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014316:Tritium
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D014316:Tritium; D008780:Methyltransferases; D006637:Histamine N-Methyltransferase