Glucose induced inhibition of radioactive nucleotide efflux from mouse pancreatic islets is dissociated from an increase in islet oxygen uptake.
Glucose at 1.7 and 5.6 mmol/l, pyruvate (30 mmol/l) and glutamine (10 mmol/l) all stimulated islet respiration slightly. More marked stimulation was achieved with glucose 8.3 mmol/l. When pyruvate (30 mmol/l) was added to islets respiring in the presence of different concentrations of glucose (1.7, 3.3 or 5.6 mmol/l), a stimulatory effect was only observed at the highest glucose concentration. Stimulation of islet oxygen uptake was also achieved when glucose 8.3 (but not 5.6) mmol/l was added to islets respiring in glutamine 10 mmol/l. Radioactive nucleotide efflux was, however, progressively inhibited by increasing the glucose concentration up to 5.6 mmol/l both in the absence or presence of glutamine 10 mmol/l. It is concluded that the glucose-induced inhibition of radioactive nucleotide efflux is not obligatorily linked to respiration.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine / Q000378:metabolism; D000818:Animals; D005947:Glucose / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010101:Oxygen Consumption / Q000187:drug effects*; D010477:Perfusion; D014316:Tritium
D000227:Adenine Nucleotides; D014316:Tritium; D005947:Glucose; D000241:Adenosine