Immunohistochemical localization of troponin-C in cultured neurons.
Our previous immunofluorescence studies on neurons have demonstrated the presence of myosin in regions of neurons which contained actin. To determine if a system similar to the troponin complex of striated muscle is present in neurons, antibody shown to be specific for the calcium-binding component of troponin (troponin-C) was applied to cultures of embryonic chick and rat dorsal root ganglia. Neurites treated with anti-troponin-C exhibited a bright fluorescence. Accompanying non-neuronal cells were less reactive than the neuronal elements. Immunodiffusion and immunofluorescence showed that the anti-troponin-C did not react with calmodulin, whereas homogenates of the ganglia elicited a positive immunochemical reaction with the anti-troponin-C in Ouchterlony tests. Our results suggest that some intra-axonal movements may be generated by the interaction of actin and myosin and controlled in part by a calcium-troponin-C-dependent mechanism.
D000818:Animals; D001369:Axons / Q000032:analysis; D002478:Cells, Cultured; D002642:Chick Embryo; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000032:analysis* / Q000166:cytology; D005779:Immunodiffusion; D009124:Muscle Proteins / Q000032:analysis*; D009475:Neurons, Afferent / Q000032:analysis*; D014336:Troponin / Q000032:analysis* / Q000276:immunology; D019209:Troponin C
D009124:Muscle Proteins; D014336:Troponin; D019209:Troponin C