Gamma interferon production by different populations of human splenic lymphocytes.
The treatment of cultures of human splenic lymphocytes with Staphylococcal enterotoxin A (SEA) resulted in the production of human gamma interferon (HuIFN-gamma). Separation of lymphocyte populations to give preparations enriched in T or B lymphocytes after removal of macrophages demonstrated that maximum IFN titers were detected when T lymphocytes were incubated with macrophages for a period of three days. The levels of IFN-gamma varied from donor to donor but the kinetics of induction were quite similar in all cases. Concentrations of 0.5-1.0 microgram/ml SEA gave optimal induction of IFN and the addition of thymosin alpha-1 at 10 micrograms/ml to the culture medium enhanced IFN-gamma production.
D002469:Cell Separation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D008214:Lymphocytes / Q000145:classification* / Q000276:immunology; D013154:Spleen / Q000166:cytology*; D013205:Staphylococcal Protein A / Q000494:pharmacology; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives / Q000494:pharmacology
D007369:Interferon Inducers; D013205:Staphylococcal Protein A; D013947:Thymosin; D007371:Interferon-gamma; D000077596:Thymalfasin