Production of human interferon-gamma in serum-free medium.
Interferon (IFN)-gamma was produced with a high yield in cultures of human peripheral mononuclear cells by combined stimulation with OK-432 and staphylococcal enterotoxin B. Human mononuclear cells cultured in serum-free medium produced several times as much IFN as those in RPMI-1640 medium containing 10% fetal bovine serum. A synergistic effect of OK-432 and staphylococcal enterotoxin B on the production of IFN-gamma was demonstrated. Ultrogel AcA54 column chromatography of crude IFN showed a single peak with an apparent molecular weight of 43,000. Our production system for human IFN-gamma offers a feasible approach to preparation of large quantities of purified IFN-gamma for structure studies, antibody production, and clinical applications.
D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D004357:Drug Synergism; D004768:Enterotoxins / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008970:Molecular Weight; D010844:Picibanil / Q000494:pharmacology
D003470:Culture Media; D004768:Enterotoxins; D010844:Picibanil; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma