Characterization of the human receptor for T-cell growth factor.
Anti-Tac monoclonal antibody has been identified as a putative antibody against the receptor for T-cell growth factor (TCGF). We now show that: (i) TCGF blocks 85% of 3H-labeled anti-Tac binding to phytohemagglutinin-activated lymphoblasts and (ii) both anti-Tac and anti-TCGF immunoprecipitate a protein band that appears to represent TCGF crosslinked to its receptor on HUT-102B2 cells. In HUT-102B2 cells, the TCGF receptor is a Mr 50,000 glycoprotein with internal disulfide bond(s) and a pI of 5.5-6.0, and it represents approximately equal to 0.05% of total cellular de novo protein synthesis. It contains a peptide of Mr 33,000 that is processed to a mature form that includes N-linked and O-linked sugars and sialic acid.
D002460:Cell Line; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D007700:Kinetics; D008223:Lymphoma / Q000276:immunology; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D009439:Neuraminidase / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000276:immunology* / Q000302:isolation & purification; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014415:Tunicamycin / Q000494:pharmacology
D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D014415:Tunicamycin; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase