Copper facilitated chemiluminescence from the sulfhydryl proteins: yeast alcohol dehydrogenase, spinach ferredoxin and metallothionein.
This paper describes a copper mediated formation of active oxygen, presumably O2, from the sulfhydryl proteins, yeast alcohol dehydrogenase spinach ferredoxin and rabbit liver metallothionein which, on the addition of cyanide in the presence of acetaldehyde, displays as a chemiluminescence. These studies may provide some insights into the mechanisms of copper toxicity and provide a sensitive assay for monitoring the presence of sulfhydryl groups.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D003300:Copper / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D008099:Liver / Q000032:analysis; D008163:Luminescent Measurements; D008668:Metallothionein / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010944:Plants; D011817:Rabbits; D015003:Yeasts
D005288:Ferredoxins; D003300:Copper; D008668:Metallothionein; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D010100:Oxygen