Comparison of the suitability of three common bacterial media for susceptibility testing of trimethoprim-sulfamethoxazole.
The in vitro inhibitory activity of trimethoprim-sulfamethoxazole is inversely related to the amount of thymine and thymidine in the test medium; manufacturers must adequately control their media to avoid spurious antimicrobial susceptibility results. No differences were observed when commercial Mueller-Hinton broth and agar were compared with a semisynthetic broth medium by the use of microdilution and disk diffusion techniques.
D000362:Agar; D003470:Culture Media / Q000032:analysis; D004755:Enterobacteriaceae / Q000187:drug effects; D013293:Enterococcus faecalis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas / Q000187:drug effects; D013420:Sulfamethoxazole / Q000494:pharmacology*; D013936:Thymidine / Q000032:analysis; D013941:Thymine / Q000032:analysis; D014295:Trimethoprim / Q000494:pharmacology*
D003470:Culture Media; D000362:Agar; D014295:Trimethoprim; D013420:Sulfamethoxazole; D013941:Thymine; D013936:Thymidine