[Synthesis and secretion of the components of the cellulase complex in Aspergillus terreus].
Aspergillus terreus was found to have two functionally different cellobiases. One of them is excreted into the surrounding medium and is a part of the cellulase complex. The second one is an endocellular (or periplasmic) enzyme. It is distinguished by its stability, the time of mRNA operation, the sorption of cell debris; moreover, its activity does not depend on changes in the parameters of the medium when the incubation mixture is replaced and the surface is treated with 0.5 M NaCl. The synthesis of all components in the cellulase complex of A. terreus is coordinated. The time it takes the mRNAs for cellulases to function does not exceed 2 h. A close correlation exists between the synthesis and secretion of polysaccharases (endoglucanases and exoglucosidases) in A. terreus. Cellobiase is adsorbed by the fungal cell wall owing to charge interactions; its desorption depends on the ionic strength and the pH of the medium.
D001230:Aspergillus / Q000201:enzymology*; D002475:Cellobiose / Q000494:pharmacology; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism
D003470:Culture Media; D002475:Cellobiose; D001617:beta-Glucosidase; D002480:Cellulase
Sintez i sekretsiia komponentov tselliulaznogo kompleksa u Aspergillus terreus.