An air pressure system for the injection of tracer substances into the brain.
A system for injecting traces and other substances into the brain through glass micropipettes using mechanically or electromechanically regulated air pressure as the driving force is described. The system has a number of advantages over currently used devices including: accurate ejection of a wide range of volumes (2 nl to several microliters); the capability to inject all currently utilized tracers; and, the direct visualization of the volume of solution injected. This system has proven reliable for a number of tract tracing experiments ranging from the injection of tracers into deep structures of the monkey brain to injecting small quantities of tracers into the brains of fetal rats.
D000399:Air Pressure; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D001931:Brain Mapping / Q000295:instrumentation*; D002415:Cats; D000882:Haplorhini; D007267:Injections / Q000295:instrumentation*; D009434:Neural Pathways / Q000033:anatomy & histology; D051381:Rats