Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA.
We have reported that production and characterization of antibodies highly specific to 5-methyl-cytosine (5mC) and the development of a sensitive immunochemical method for the detection of 5mC in DNA [FEBS Lett. (1982) 150, 469]. Extension of this method to two other modified bases, 6-methyladenine (6mA) and 7-methylguanine (7mG), is reported here. By use of this immunochemical approach, we are able to detect 5mC, 6mA and 7mG in human and Drosophila DNA and confirm their presence in the DNA of two mealybug species.
D044503:5-Methylcytosine; D000225:Adenine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D000818:Animals; D000906:Antibodies; D055598:Chemical Phenomena; D002621:Chemistry; D003596:Cytosine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004247:DNA; D004331:Drosophila melanogaster; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D006801:Humans; D007120:Immunochemistry; D007313:Insecta; D008297:Male; D010920:Placenta / Q000378:metabolism; D051381:Rats; D013045:Species Specificity; D013094:Spermatozoa / Q000378:metabolism
D044503:5-Methylcytosine; D000225:Adenine; D000906:Antibodies; D003596:Cytosine; D004247:DNA; D006147:Guanine; C008450:7-methylguanine; C000730567:6-methyladenine