Suppression of the neutral protease activity of macrophages treated with asbestos in vitro.
Macrophages are often conspicuous in asbestos-induced inflammatory lesions. Chrysotile type B elicits macrophages into the peritoneal cavity of mice which produce high levels of the neutral protease, plasminogen activator; in vitro addition of these same fibers to mouse peritoneal macrophages stimulates enzyme production. It is reported here that, for endotoxin-elicited mouse peritoneal macrophages fed chrysotile type B in vitro, the increased plasminogen activator activity is suppressed by low concentrations of anti-inflammatory steroids. Other active drugs include colchicine and vinblastine. These studies are considered important, as they suggest an approach to controlling the levels of a potentially deleterious enzyme system (PA-plasmin) from macrophages treated with asbestos fibers.
D000818:Animals; D001194:Asbestos / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology; D005343:Fibrinolytic Agents; D005938:Glucocorticoids / Q000494:pharmacology; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015849:Plasminogen Inactivators; D014747:Vinblastine / Q000494:pharmacology
D005343:Fibrinolytic Agents; D005938:Glucocorticoids; D015849:Plasminogen Inactivators; D001194:Asbestos; D014747:Vinblastine; D009113:Muramidase; D010960:Plasminogen Activators; D003078:Colchicine