Development of anti-pp60src serum with antigen produced in Escherichia coli.
We have purified p60(src) from bacterial recombinants which direct the synthesis of the Rous sarcoma virus transforming gene (src) product. This protein was injected into rabbits, and they produced a highly cross-reactive serum which can recognize the src protein from many different strains of Rous sarcoma virus.
D003429:Cross Reactions; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D015688:Oncogene Protein pp60(v-src); D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology*
D004274:DNA, Recombinant; D007106:Immune Sera; D014764:Viral Proteins; D015688:Oncogene Protein pp60(v-src)