Two macromolecular complexes between alkaline phosphatase and immunoglobulin A in a patient's serum.
We report the presence of two separate macromolecular complexes between an immunoglobulin and alkaline phosphatase (EC 3.1.3.1) in the serum of a patient with cholestatic liver disease. The complexes were seen as slow-moving bands on electrophoresis on polyacrylamide gel (70 g/L). Estimates of relative molecular mass for the two bands were 785 000 and 490 000 by gel chromatography on Sephacryl S300, and 640 000 and 420 000 by gradient gel-electrophoresis. Inhibitor and heat-inactivation studies showed that the two bands had similar properties, resembling the liver isoenzyme but more heat stable. Immunological studies suggested that both bands consisted of a complex of liver alkaline phosphatase with kappa type immunoglobulin A.
D000368:Aged; D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D002779:Cholestasis / Q000097:blood*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007145:Immunoglobulin kappa-Chains / Q000302:isolation & purification; D007527:Isoenzymes / Q000302:isolation & purification*; D008970:Molecular Weight; D009439:Neuraminidase
D007070:Immunoglobulin A; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D000469:Alkaline Phosphatase; D009439:Neuraminidase