Enhancement of the spontaneous development of autoreactive B cells by PPD in mouse peritoneal cell cultures.
The effect of tuberculin purified protein derivative (PPD) on the development of plaque-forming cells (PFC) against bromelain-treated syngeneic mouse red blood cells (Br-MRBC) was studied in peritoneal cell cultures. The finding that PPD enhances the development of PFC to Br-MRBC, even under conditions where cell division is blocked by mitomycin C treatment, suggests that cell proliferation does not represent a necessary prerequisite for differentiation of precursor cells into autoantibody-forming cells.
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D066298:In Vitro Techniques; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D014373:Tuberculin / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D008937:Mitomycins; D014373:Tuberculin; D016685:Mitomycin