High-performance liquid chromatography of staphylococcal enterotoxin B.
Size-exclusion, ion-exchange, and reversed-phase chromatography were investigated for use in purification and characterization of Staphylococcal enterotoxin B (SEB), a 28,000 Mr protein associated with Staphylococcal food-borne intoxication. In all approaches chromatography of crude or purified SEB yielded one or more components which displayed toxin activity and contained the 28,000 Mr SEB protein plus other lower-molecular-weight protein species. Reversed-phase chromatography of detergent-treated SEB permitted resolution of the 28,000 Mr protein from lower-molecular-weight components.
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification*; D005516:Food Microbiology; D006801:Humans; D005779:Immunodiffusion; D008970:Molecular Weight; D013203:Staphylococcal Infections / Q000209:etiology
D001426:Bacterial Proteins; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal