Immunocytochemical localization of a histone H2A variant in the mammalian nucleolar chromatin.
The distribution of protein "A", a minor variant of H2A present in the mouse testis, was studied in the liver and brain nuclei using peroxidase-antiperoxidase technique. The data presented here suggest that nucleolar-associated chromatin is highly enriched in protein "A". Microspectrophotometric measurements corroborate the immunocytochemical data. The regional differentiation in the eukaryotic chromatin, therefore, may involve qualitative changes in the histone composition.
D000818:Animals; D001923:Brain Chemistry; D002466:Cell Nucleolus / Q000032:analysis*; D002843:Chromatin / Q000032:analysis*; D006651:Histocytochemistry; D006657:Histones / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D013737:Testis / Q000032:analysis
D002843:Chromatin; D006657:Histones