Chromatography of proteases on agar column.
Agar column was used for the isolation and purification of trypsin and pancreatic proteases. Ballast proteins were eluted with water while the absorbed protease was eluted with 1 M sodium chloride solution. The capacity is approximately 1.8 mg of pure trypsin per ml of the packed agar.
D000362:Agar; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D010179:Pancreas / Q000201:enzymology; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D014357:Trypsin / Q000302:isolation & purification
D000362:Agar; D010447:Peptide Hydrolases; D014357:Trypsin