Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
A technique for purifying rat monoclonal antibodies quickly and efficiently from in vitro culture supernatants is described. It is based on the fact that more than 95% of rat immunoglobulins carry kappa light chains. A mouse monoclonal antibody with suitable binding affinity for rat kappa light chains is immobilized on solid support and used to purify rat immunoglobulins. Milligrams of rat monoclonal antibodies may be rapidly concentrated from culture supernatants with high recovery. Rat monoclonal antibodies expressing lambda light chains (about 5% of the total) may be purified in a similar way with an appropriate anti-rat lambda chain monoclonal antibody.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D006241:Haptens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology; D010321:Parvoviridae / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004136:Dinitrobenzenes; D006241:Haptens; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins