Bovine x mouse hybridomas that secrete bovine immunoglobulin G1.
The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting bovine immunoglobulins. One of these lines has continued to secrete immunoglobulin G1 (5 to 10 micrograms per milliliter) for over 16 months. The bovine x mouse hybrid cells can be expected to provide bovine monoclonal immunoglobulins for sequencing studies and for use as serological standards as well as to provide messenger RNA for cloning bovine immunoglobulin genes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002417:Cattle; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M