Monoclonal antibodies against horseradish peroxidase.
Four hybridomas making monoclonal antibodies against horseradish peroxidase have been established. Two of them are IgM and two are IgG1 with kappa light chains. Antibody HP-03 has been selected as the most suitable one for preparation of the peroxidase-anti-peroxidase complex. Furthermore, optimal conditions for construction and applications of the PAP complex in immunocytochemical and immunohistochemical methods are shown.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D006735:Horseradish Peroxidase / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007526:Isoelectric Point; D010544:Peroxidases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006735:Horseradish Peroxidase; D010544:Peroxidases