Preparation of factor VIII-free plasma by immunoaffinity chromatography on insolubilized antibodies against factor VIII-related antigen.
A method is designed to prepare factor VIII-free plasma in large amounts. It is obtained from normal human plasma by immunoaffinity chromatography on insolubilized antibodies against factor VIII-related antigen. Its use in the routine assay of VIII procoagulant activity is well established by testing various samples ranging from 0 to 7 U/ml.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D001780:Blood Coagulation Tests; D002846:Chromatography, Affinity; D005169:Factor VIII / Q000032:analysis* / Q000276:immunology*; D006801:Humans; D008722:Methods; D011817:Rabbits; D014841:von Willebrand Factor
D000906:Antibodies; D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII