Functional identity between murine gamma interferon and macrophage activating factor.
We have previously suggested that two lymphokine activities-macrophage activating factor (MAF) and gamma interferon (IFN-gamma)-are mediated by the same molecule. Striking similarities were noted in their cellular biosynthesis, rate of inactivation with acid treatment and heating, and elution profiles on Sephacryl S-200. In addition, highly specific polyclonal antibodies to murine IFN-gamma neutralized MAF and IFN-gamma to a similar degree. However, definitive studies require a pure product and we now report that murine IFN-gamma that had been cloned and expressed in a simian nonlymphoid cell line shows MAF activity. But it is not yet known whether IFN-gamma is responsible for all the MAF activity in media conditioned by T cells as the possibility for MAF heterogeneity remains.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D007371:Interferon-gamma / Q000502:physiology*; D008222:Lymphokines / Q000502:physiology*; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000502:physiology*; D051379:Mice
D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007371:Interferon-gamma