Effect of different amounts of arachidonic acid on vessel wall-generated PGI2 and TXA2.
It has been suggested that low concentrations of AA may have vasoprotective and high concentrations vaso-damaging effects. To relate these effects to vascular generation of PGI2 and TXA2, we incubated human umbilical vein rings with AA (0, 0.01, 0.1, 1 and 2 mM) and examined the supernates for 6-keto-PGF1 alpha and TXB2. Low concentrations of AA (0.01 and 0.1 mM) caused preferential and maximal PGI2 release, whereas higher concentrations (1 and 2 mM) resulted in marked and preferential increase in TXA2 release. Disequilibrium in vascular PGI2 and TXA2 release towards the latter may relate to vaso-damaging effects of high concentrations of AA.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D001808:Blood Vessels / Q000187:drug effects* / Q000502:physiology; D011464:Epoprostenol / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D011247:Pregnancy; D013928:Thromboxane A2 / Q000096:biosynthesis* / Q000378:metabolism; D013931:Thromboxanes / Q000096:biosynthesis*; D014471:Umbilical Veins
D001095:Arachidonic Acids; D013931:Thromboxanes; D016718:Arachidonic Acid; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol