A study of DNA melting in concentrated water-alcohol solutions.
The DNA melting profiles with high resolution have been studied for conditions corresponding to the B and A conformations of DNA in water-alcohol solutions. The melting profiles of the A-form and B-form DNA, their mean melting temperatures and melting range width were found to differ. DNA was shown to be heterogeneous in respect of the B-A transition, the GC-rich regions more readily converting into the A form than AT-rich ones. The presence of boundaries between the A and B sections within the transition zone did not smooth off the fine structure of melting profiles.
D004247:DNA; D000431:Ethanol; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012996:Solutions; D013696:Temperature; D014270:Trifluoroethanol; D014867:Water
D012996:Solutions; D014867:Water; D000431:Ethanol; D014270:Trifluoroethanol; D004247:DNA