Structure determination of the major asparagine-linked sugar chain of human factor VIII--von Willebrand factor.
N-glycosidically-linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were separated by high-voltage electrophoresis. Five fractions were obtained, one of them representing 60% of the total amount of the N-glycosidically-linked glycans of FVIII/vWf. On the basis of the carbohydrate composition, methylation analysis and 500 MHz 1H-NMR spectroscopy, we describe the primary structure of this major glycan which is of the monosialylated and monofucosylated biantennary N-acetyllactosaminic type.
D001216:Asparagine; D001779:Blood Coagulation Factors / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D006801:Humans; D006834:Hydrazines; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
D001779:Blood Coagulation Factors; D002241:Carbohydrates; D006834:Hydrazines; D009844:Oligosaccharides; D014841:von Willebrand Factor; D001216:Asparagine; D005169:Factor VIII