Regulatory pattern of the Escherichia coli lysA gene: expression of chromosomal lysA-lacZ fusions.
The regulation of lysA which encodes the last enzyme for lysine biosynthesis in Escherichia coli, diaminopimelic acid-decarboxylase, was studied by using lysA-lacZ fusions. Our results indicate an absolute requirement for the LysR product for its activation, LysR protein present in a limiting amount which can be titrated by a multicopy plasmid carrying its target site and a negative regulatory role for the LysA protein itself which decreases lysA-lacZ expression 30-fold.
D001426:Bacterial Proteins; D002262:Carboxy-Lyases / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007763:Lac Operon; D008239:Lysine / Q000096:biosynthesis*; D010957:Plasmids
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D002262:Carboxy-Lyases; C079912:LysA protein, Bacteria; C020031:diaminopimelic acid decarboxylase; D008239:Lysine