Determination of relative antigen-antibody avidities by radial immunodiffusion.
Radial immunodiffusion can be used to determine relative antigen-antibody avidities in exactly the same way as demonstrated previously for quantitative immunoelectrophoresis (Birkmeyer et al., 1981). Antigen-antibody interactions of greater avidity result in a greater value of (delta Area/delta [Antigen]) in plots of immunoprecipitin circle area versus antigen concentration while interactions of equal avidity will yield equal values of (delta Area/delta [Antigen]). This was demonstrated using antigens of different weight ranging from 8000 to 66,000.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D006441:Hemoglobin A / Q000032:analysis / Q000276:immunology*; D007106:Immune Sera; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis / Q000379:methods; D010944:Plants; D014361:Trypsin Inhibitors / Q000032:analysis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D014361:Trypsin Inhibitors; D006441:Hemoglobin A