Pyruvate kinase type M2 is phosphorylated in the intact chicken liver cell.
Isolated hepatocytes from 24 h starved chicks were depleted of phosphate and incubated in the presence of 32P-orthophosphate. Pyruvate kinase type M2 from crude cell extracts was partially purified by chromatography on DEAE-Sephacel and hydroxylapatite. SDS slab gel electrophoresis of the fractions containing the enzyme and immunoprecipitation with antisera showed the phosphorylation of pyruvate kinase type M2. Phosphoamino acid analysis identified serine as phosphate acceptor.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011770:Pyruvate Kinase / Q000378:metabolism*; D012694:Serine / Q000378:metabolism
D000596:Amino Acids; D007527:Isoenzymes; D012694:Serine; D011770:Pyruvate Kinase