Cold-stable microtubules and microtubule-organizing centres in astrocytes in primary culture.
Immunocytochemical staining with anti-tubulin antibodies showed cold-stable microtubules in astrocytes in culture. On rewarming, microtubule-organizing centre (MTOC) could be visualized. MTOCs showed structural complexity, from 2-3 fibres to a mass of interweaving fibres. Only one MTOC per cell was found in cultures of both polygonal and stellate-bearing cells.
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D002478:Cells, Cultured; D003080:Cold Temperature; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000502:physiology*; D051381:Rats