Leukotriene C promotes prostacyclin synthesis by human endothelial cells.
Cultured endothelial cells from human umbilical vein were labelled with [3H]arachidonic acid for 16 hr. The radiolabel was localized primarily in phospholipids (93%) and 73% was distributed equally between phosphatidylcholine and phosphatidylethanolamine. Leukotriene C (10-1,000 nM) promoted a dose-dependent release of radiolabel into the culture medium. This response was 3.3 times control values at 100 nM. The major arachidonic acid metabolite synthesized was prostacyclin, which was 33% of the total released radiolabel. Endothelial cells also released small amounts of prostaglandin F2 alpha (6.1%), unidentified lipoxygenase products (14.8%), and unreacted arachidonic acid (33%). The 30-min time course of release was independent of the leukotriene C concentration used. Leukotriene D at similar concentrations also promoted endothelial cells to release primarily prostacyclin and unreacted arachidonic acid. The release of prostacyclin, a potent vasodilator agent, may be an important mediator in slow reacting substance effects on the vasculature.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D004727:Endothelium / Q000187:drug effects / Q000502:physiology; D011464:Epoprostenol / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D010743:Phospholipids / Q000096:biosynthesis; D011247:Pregnancy; D011453:Prostaglandins / Q000096:biosynthesis*; D013189:SRS-A / Q000494:pharmacology*; D014316:Tritium; D014471:Umbilical Veins / Q000187:drug effects / Q000502:physiology*
D001095:Arachidonic Acids; D010743:Phospholipids; D011453:Prostaglandins; D013189:SRS-A; D014316:Tritium; D016718:Arachidonic Acid; D011464:Epoprostenol