Immunohistochemical localization of intestinal phospholipase A2 in rat paneth cells.
Using the peroxidase-anti-peroxidase (PAP) technique with a specific rabbit anti-swine intestinal-phospholipase-A2 serum, the immunoreactivity of this phospholipase A2 was localized in rat-intestinal Paneth cells. The specific rabbit anti-swine intestinal-phospholipase-A2 serum did not stain the rat-pancreatic acinar cells which were stained by a specific rabbit anti-swine pancreatic-phospholipase-A2 serum. Specific rabbit anti-swine pancreatic-phospholipase-A2 serum did not stain rat-intestinal Paneth cells. Therefore, there is no cross-immunoreactivity between pancreatic and intestinal phospholipases.
D000818:Animals; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000201:enzymology; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis*; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains
D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2