Insulin binding on MOLT 4 cells: effect of a sulfonylurea.
Cultured neoplastic cell lines are widely considered an adequate model for insulin receptor studies. In this work the insulin receptor of a well-known continuous T-cell line (MOLT 4) was characterized. These cells showed specific insulin receptor with binding properties quite similar to those of other lymphoblastoid cells. Therefore, we used MOLT 4 receptor to evaluate the effect of the sulfonylurea glipizide on insulin binding. After a 24-hour preliminary incubation of cells with glipizide, we found a 44% increase of insulin receptor binding apparently due to an increase of insulin binding sites.
D002460:Cell Line; D005913:Glipizide / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D011972:Receptor, Insulin / Q000187:drug effects*; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000378:metabolism
D007328:Insulin; D013453:Sulfonylurea Compounds; D011972:Receptor, Insulin; D005913:Glipizide