A simple method of analyzing profiles in time-difference direct optical scanning gel chromatography.
A simple method is presented for the analysis of time-difference, large-zone chromatography profiles. The method is derived from basic theory and is applicable to multicomponent as well as single-component systems. Simple computer simulations are used to demonstrate the inaccuracies of earlier, more empirical methods. This method has been tested on several proteins using an inexpensive, semi-automated, data acquisition and control system.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000032:analysis; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002845:Chromatography / Q000379:methods; D006801:Humans; D008838:Microcomputers; D009211:Myoglobin / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D010649:Phenylalanine / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis
D001798:Blood Proteins; D009211:Myoglobin; D011506:Proteins; D012710:Serum Albumin, Bovine; D010649:Phenylalanine; D010047:Ovalbumin; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase