Metabolism of [14C]glucose by postimplantation mouse embryos in vitro.
Carbon dioxide and lactate production from [14C]glucose were measured for post-implantation mouse embryos aged 6 to 9 1/2 days post coitum in static cultures with a defined medium. The rate of metabolism increased rapidly and paralleled the increase in protein content indicating a fairly uniform rate of metabolism throughout the period. At all stages studied more than 90% of the glucose utilized was converted to lactate. Over a quarter of carbon dioxide produced was derived from the C-1 position resulting in high C-1:C-6 ratios, indicating that the Pentose Phosphate Shunt is a major oxidative pathway. The influence of various culture condition on CO2 production showed that high concentrations of glucose did not affect glucose utilization whilst high lactate concentrations had a significant inhibitory effect. Pyruvate had no discernible effect.
D000818:Animals; D002245:Carbon Dioxide / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007773:Lactates / Q000096:biosynthesis; D051379:Mice; D009924:Organ Culture Techniques; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism
D007773:Lactates; D011506:Proteins; D002245:Carbon Dioxide; D005947:Glucose