Serum and plasma stimulate prostaglandin production by alveolar macrophages.
Fetal bovine serum (FBS) stimulated rabbit alveolar macrophages to synthesize prostaglandins (PG) and release lysosomal enzymes. This stimulatory action was not entirely due to the effect of foreign protein in FBS, since rabbit serum and plasma, both homologous and autologous, also induced release of PGs and lysosomal enzymes. Rabbit serum and plasma are less effective than FBS as a stimulus for PG release, with rabbit serum being more potent than plasma at the same concentration. Bovine serum albumin elicited a dose-dependent increase of arachidonic acid release by macrophages, but not of PG production. Hence, the fatty acid "trapping" effect of albumin in serum and plasma is not responsible for the PG stimulation. The PG stimulating factors were stable at 56 degrees C for 30 min., but lost half the activity after heating at 100 degrees C for 10 min. Gel permeation chromatography of FBS showed several peaks of PG stimulating and arachidonic acid releasing activity. The molecular weight of the major one (150,000 daltons) is similar to that of immunoglobulin G. Rabbit IgG, when added to the macrophage culture, stimulated release of arachidonic acid and PGs. However, the major stimulatory effect in serum or plasma is not all due to IgG, since removal of IgG by a Protein A-agarose column did not remove the stimulatory effect of FBS and rabbit serum. The possibility of other factors, such as complement fragments, is discussed.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001790:Blood Physiological Phenomena; D002417:Cattle; D005966:Glucuronidase / Q000378:metabolism; D007074:Immunoglobulin G / Q000302:isolation & purification; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011453:Prostaglandins / Q000096:biosynthesis*; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay
D001095:Arachidonic Acids; D007074:Immunoglobulin G; D011453:Prostaglandins; D016718:Arachidonic Acid; D005966:Glucuronidase