Synthesis of thymosin alpha 1 precursor cDNA and purification of active mRNA by affinity chromatography.
1. Thymosin alpha 1 precursor [3H]cDNA-cellulose synthesis was carried out by reverse transcription with RNA-dependent DNA polymerase from avian myeloblastosis virus using oligo(dT)-bound to cellulose as a primer. 2. Unlabelled thymosin alpha 1 precursor cDNA-cellulose was synthetized in a preparative scale to be used in affinity chromatography. 3. Poly(A)-mRNA from calf thymus was subjected to cDNA-cellulose affinity chromatography and an active messenger obtained in a yield of 3% respect to the total thymus poly(A)-mRNA chromatographied. 3. The analysis of the translation products of the purified mRNA have shown it as the thymosin alpha 1 precursor messenger.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D002846:Chromatography, Affinity; D004247:DNA / Q000138:chemical synthesis*; D008239:Lysine / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D013698:Templates, Genetic; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives / Q000235:genetics*; D013951:Thymus Hormones / Q000235:genetics*
D011498:Protein Precursors; D012333:RNA, Messenger; D013951:Thymus Hormones; D013947:Thymosin; D004247:DNA; D008239:Lysine; D000077596:Thymalfasin