Identification of monkey lung procathepsin D-II as a pepsinogen-C-like acid protease zymogen.
Procathepsin D-II (Mr = 37 500) was purified from Japanese monkey lung at pH 7.0, and was shown to be converted to the active form, cathepsin D-II (Mr = 33 000) via an intermediate (Mr = 35 500) upon treatment at pH 3.0 and 14 degrees C. Procathepsin D-II was shown to be the inactive precursor of cathepsin D-II based on the following results: the former was inactive toward heat-denaturated casein at pH 5.4 whereas the latter was active; the former was not inactivated by diazoacetyl-DL-norleucine methyl ester in the presence of Cu2+ ion at pH 6.0 whereas the latter was inactivated rapidly under the same conditions; and the former had no affinity to pepstatin-Sepharose between pH 5 and 7 whereas the latter was adsorbed to it. With a rabbit antiserum against procathepsin D-II, cathepsin D-II, pepsinogen C and pepsin C of Japanese monkey were each found to give a single precipitin line which fused completely with each other on agarose plate. On the other hand, cathepsin D-I purified from the monkey lung, and pepsinogens A (I, II, III-1, III-2 and III-3) obtained from the monkey gastric mucosa failed to precipitate with the antiserum. With the antiserum against the monkey pepsinogen C, the same results were obtained. Further, procathepsin D-II and pepsinogen C were shown to have the same amino-terminal amino acid sequence, Ala-Val-Val-Lys-Val-Pro-Leu-Lys-Lys-Phe-Lys-. All these results indicate a strong similarity of procathepsin D-II and cathepsin D-II to pepsinogen C and pepsin C, respectively.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002402:Cathepsin D; D002403:Cathepsins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry; D008168:Lung / Q000201:enzymology*; D008251:Macaca; D010434:Pepsin A; D010435:Pepsinogens; D010447:Peptide Hydrolases; D011817:Rabbits; D013329:Structure-Activity Relationship
D000596:Amino Acids; D004792:Enzyme Precursors; D010435:Pepsinogens; D002403:Cathepsins; D010447:Peptide Hydrolases; D010434:Pepsin A; D002402:Cathepsin D