The use of a B subtilis in a pre-incubation assay for the detection of dna-modifying agents.
A microbial bioassay for the detection of agents which induce increased lethal damage in DNA repair deficient strains of B. subtilis was modified and evaluated using six established mutagens. This modified procedure used a liquid pre-incubation technique with the plate incorporation assay allowing detection of mutagenic compounds which require metabolic activation and are sparingly soluble in water.
D001412:Bacillus subtilis / Q000235:genetics*; D004260:DNA Repair; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens
D009153:Mutagens