Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.
A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.
D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D046911:Macromolecular Substances; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D055503:Protein Multimerization; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000302:isolation & purification
D000546:Amanitins; D046911:Macromolecular Substances; D012319:RNA Polymerase II