Fine resolution of the poly ADP-ribosylated domains of polynucleosomal chromatin: DNA gene and integrity analysis; mechanism of histone H1 modification.
The focus of our laboratory has been to ascertain how the poly(ADP-ribosyl)ation reaction influences the structure and biological function of nucleosomal chromatin. Antibody to poly(ADP-ribose (ADP-Rib] was coupled to Sepharose to prepare an immunoaffinity column. The following new information concerning poly(ADP-Rib) and chromatin was obtained with this column: 1) those limiting domains of nucleosomal chromatin undergoing the modification (circa 10%) could be isolated in the "bound" fraction, 2) antibody-bound nucleosomes contained all the poly ADP-ribosylated acceptors and polymerase activity of the bulk chromatin, 3) bound nucleosomes contain significant numbers of internal DNA strand breaks, and [3H]-thymidine (TdR) repair incorporation from in vivo "DNA repair label," compared to the unbound, bulk of the chromatin, 4) the presence of actively transcribed genes in "bound" nucleosomes is being investigated, 5) the acetylation modification of histones occurs in the same domains of chromatin as does poly(ADP-ribosyl)ation, and 6) poly ADP-ribosylated histone H1 can be selectively purified by the immunoaffinity method. These same histone H1 molecules appear to be equally accessible to the histone kinase, phosphorylation modification. In addition, new information has been obtained concerning histone H1 cross-linking by poly(ADP-ribosyl)ation, and on polymerase binding sites to chromatin. We have reconstituted histone H1-depleted chromatin with intact H1 and peptide domains of H1, and subsequently studied H1-poly(ADP-Rib)-complex synthesis. The data indicate that elongation of poly(ADP-Rib) proceeds on the amino terminal region of this histone. By utilizing the new techniques of DNA technology, huge advances in our understanding of the programmed structure of the eukaryotic genome have been accomplished in a relatively short time. To complement this explosion of information, it is important to ascertain how these recently appreciated properties of eukaryotic DNA are packaged within extended and condensed domains of chromatin. The research to be discussed is directed, in part, at this latter topic. We have initiated a program aimed at furthering our comprehension of chromatin structure by studying one specific, enzymatically active chromosomal protein.
D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin; D002846:Chromatography, Affinity; D004260:DNA Repair; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D066298:In Vitro Techniques; D009702:Nucleoside Diphosphate Sugars / Q000032:analysis*; D009707:Nucleosomes / Q000032:analysis* / Q000276:immunology; D011064:Poly Adenosine Diphosphate Ribose / Q000032:analysis* / Q000276:immunology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
D002843:Chromatin; D006657:Histones; D009702:Nucleoside Diphosphate Sugars; D009707:Nucleosomes; D011064:Poly Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases