Post-transcriptional control of L-1,2-propanediol oxidoreductase in the L-fucose pathway of Escherichia coli K-12.
During anaerobic growth on L-fucose, Escherichia coli excretes L-1,2-propanediol formed by an inducible NAD-linked oxidoreductase. The activity of this enzyme is highly induced by L-fucose only anaerobically. However, in strains bearing a hybrid operon with the promoter of fucO (the propanediol oxidoreductase gene) fused to lacZYA, the beta-galactosidase activity is inducible by fucose both anaerobically and aerobically. In merodiploids bearing both fucO+ and phi(fucO-lac), propanediol oxidoreductase is inducible only anaerobically, but beta-galactosidase remains inducible both aerobically and anaerobically. Thus, the absence of respiratory control in the expression of phi(fucO-lac) cannot be attributed to a polarity effect of the fusion on a gene encoding a protein with autogenous regulatory function in transcription. The respiratory effect on the induced propanediol oxidoreductase activity is therefore post-transcriptional.
D000332:Aerobiosis; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D000693:Anaerobiosis; D004171:Diploidy; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005643:Fucose / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D007763:Lac Operon; D011499:Protein Processing, Post-Translational; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D005643:Fucose; D000429:Alcohol Oxidoreductases; C021494:lactaldehyde reductase; D001616:beta-Galactosidase