Partial separation of blood clotting factors, albumin, and IgG by continuous free film electrophoresis.
Blood plasma and factor VIII concentrate have been fractionated by continuous free film electrophoresis in an apparatus which was designed to overcome the problems of overheating usually inherent in this technique. Results show that complete separation of plasma proteins is unlikely to be obtained by a single passage through this apparatus. However, a useful degree of fractionation may be obtained with certain proteins, for example fibrinogen and IgG. It should be possible to concentrate blood clotting factors if the electrophoresis is used in combination with a conventional separation procedure such as adsorption on aluminium hydroxide. The advantage of the electrophoretic technique is that it is a continuous process using the minimum of extraneous chemicals. The disadvantage is that it introduces a 40-fold dilution into the fractionation.
D000818:Animals; D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D001797:Blood Protein Electrophoresis / Q000295:instrumentation / Q000379:methods; D005591:Chemical Fractionation; D005169:Factor VIII / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D011817:Rabbits; D012709:Serum Albumin / Q000302:isolation & purification*; D013997:Time Factors
D001779:Blood Coagulation Factors; D007074:Immunoglobulin G; D012709:Serum Albumin; D005169:Factor VIII