Monoclonal antibody to human cytotrophoblast cross-reacts with basal layers of some fetal epithelial surfaces.
A monoclonal antibody (I8B/A5) was generated towards human first-trimester purified trophoblast plasma membrane which, unlike others reported in the literature, reacted only against cytotrophoblast but not against syncytiotrophoblast. However, this antibody cross-reacted with certain fetal epithelial and endothelial surfaces in a well-defined manner. On such stratified epithelium as fetal skin, only the basal layers of cells were stained, and this was therefore analogous to the pattern observed in chorionic villi where only underlying cytotrophoblast and not superficial syncytiotrophoblast reacted with I8B/A5. These observations suggest that the target antigen either could be a component of certain structures at these sites, such as basement membrane, or it is a marker for proliferating and undifferentiated cells, which would make it an interesting molecule for developmental biologists.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D004848:Epithelium / Q000276:immunology; D005321:Extraembryonic Membranes / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000276:immunology*
D000911:Antibodies, Monoclonal