Arginine-induced dispersion of cells from neonatal rat islets of Langerhans.
Neonatal rat islets of Langerhans respond in vitro to prolonged arginine exposure by cellular dissociation and a cell monolayer formation resulting in complete dissolution of the islet structure. This effect is seen at an arginine concentration of 10 mM and a minimum exposure of 8 hr. The dissociated cells remain viable in culture for at least 3 weeks though there is no response to a glucose challenge following the first week. Leucine, another amino acid which initiates hormone release, does not alter islet morphology. The arginine-induced alteration in the islet structure may be due to an effect on the factors which control cellular adhesion.
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007930:Leucine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D005934:Glucagon; D001120:Arginine; D007930:Leucine; D005947:Glucose