Enzyme-linked immunosorbent microtiter assay for red cell serology.
Enzyme-linked immunoabsorbent assays (EIA) permit quantitation and automation of reactions. Previous reports have also shown improved sensitivity for red cell antibodies. We have developed a microtiter technique for EIA, thus decreasing the amounts of reagents required. EDTA was used rather than NaOH to stop the enzyme reaction allowing more precise timing of reactions and improved quantitation of large batches of tests. As in prior reports, the sensitivity was slightly greater than the antiglobulin test, but occasional red cell antibodies failed to show specificity by EIA. The use of microtiter plates and EDTA decreased the steps and reagents required and has the potential for automation of at least the optical reading of such assays in the blood bank.
D004492:Edetic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000145:classification / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007518:Isoantibodies; D008722:Methods; D008832:Microchemistry
D007518:Isoantibodies; D004492:Edetic Acid