Triple fixation of Bacillus subtilis dormant spores.
A triple-fixation method with a sequential application of 5% glutaraldehyde, 1% osmium tetroxide, and 2% potassium permanganate gave superior preservation of the ultrastructure of Bacillus subtilis dormant spores with a thick spore coat.
D001412:Bacillus subtilis / Q000502:physiology / Q000648:ultrastructure*; D005404:Fixatives; D005976:Glutaral; D008854:Microscopy, Electron; D009993:Osmium Tetroxide; D011196:Potassium Permanganate; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure
D005404:Fixatives; D011196:Potassium Permanganate; D009993:Osmium Tetroxide; D005976:Glutaral