[Anticoagulating properties of fungal proteases tricholysine (triase) and longolytin].
Tricholysine (triase) and longolytin, isolated from cultural fluid of two similar lower fungi Trichothecium roseum and Arthrobotrys longa, exhibited anticoagulant and fibrinolytic properties after addition to blood plasma of experimental animals. Intravenous administration of the enzymes into animals caused the following effects: an increase in fibrinolytic activity, a slight decrease of fibrinogen concentration, an inadequate elongation of recalcification time and of thrombin time. Analysis of thromboelastograms demonstrated the anticoagulant effect: index of coagulation and maximal amplitude were decreased, time of coagulation was elongated. Preparation of tricholysine exhibited low toxicity; only doses, which exceeded 80-fold the thrombolytic dose, caused death in 34% of mice after intravenous administration.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000494:pharmacology* / Q000633:toxicity; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010447:Peptide Hydrolases / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D013916:Thrombelastography; D013918:Thrombin Time
D005343:Fibrinolytic Agents; D010447:Peptide Hydrolases; C037962:longolytin; C014864:tricholysine
Antikoaguliantnye svoĭstva gribnykh proteaz trikholizina (triazy) i longolitina.