Vibrio cholerae soluble hemagglutinin/protease is a metalloenzyme.
A soluble hemagglutinin/protease produced by Vibrio cholerae, which has previously been shown to hydrolyze fibronectin and ovomucin and to cleave lactoferrin and the A subunit of the heat-labile enterotoxin of Escherichia coli, appears to be a zinc metalloendopeptidase. Both its hemagglutinative and protease functions are inhibited by chelating agents, including Zincov, a hydroxamic acid derivative specifically designed to inhibit zinc metalloproteases. Thermolysin, a known zinc-containing protease, also causes hemagglutination of responder chicken erythrocytes. This activity is inhibited by Zincov, which does not affect the hemagglutination activity of trypsin and pronase. The hemagglutinin/protease is active on furylacryloyl-Gly-Leu-NH2, a synthetic substrate for thermolysin and other similar proteases. The hemagglutination activity of V. cholerae-infected or cholera toxin-treated infant rabbit intestinal fluid is not inhibited by Zincov, which suggests that this activity is not due to the hemagglutinin/protease, as formerly proposed.
D002122:Calcium Chloride / Q000494:pharmacology; D002712:Chlorides; D004492:Edetic Acid / Q000494:pharmacology; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D006388:Hemagglutinins; D007700:Kinetics; D008666:Metalloendopeptidases; D011480:Protease Inhibitors / Q000494:pharmacology; D014734:Vibrio cholerae / Q000201:enzymology / Q000276:immunology*; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D002712:Chlorides; D006388:Hemagglutinins; D011480:Protease Inhibitors; D017967:Zinc Compounds; C016837:zinc chloride; D004492:Edetic Acid; D010450:Endopeptidases; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease; D015032:Zinc; D002122:Calcium Chloride