Regulation of plasminogen-activator in mastocytoma cells by lymphokines.
Culture supernatants from mitogen-stimulated splenocytes were found to stimulate protease production in P815Y mastocytoma cells. Such supernatants increased cell-associated plasminogen activator levels in a dose-dependent fashion, and under serum-free conditions. In contrast to peritoneal exudate cells, tumor-cell plasminogen activator was not enhanced by the mitogen ConA alone. The tumor cell line P815Y may, thus, be used as a homogeneous cell source for the quantitation of lymphocyte factors which activate or inhibit plasminogen activator activity.
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D008222:Lymphokines / Q000502:physiology*; D012515:Mast-Cell Sarcoma / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010960:Plasminogen Activators / Q000096:biosynthesis*
D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A; D010960:Plasminogen Activators