[Structural changes in the contractile proteins of muscle fiber studied by polarization ultraviolet fluorescence microscopy. VII. The effect of Ca2+ on the nature of the conformational changes in F-actin induced by the binding of heavy meromyosin].
Changes in anisotropy of tryptophan fluorescence and in birefringence of actin filaments induced by the binding to actin of heavy meromyosin (HMM), both containing DTNB light chains and devoid of them, were found in rabbit muscle fibres free of myosin, troponin, and tropomyosin. Ca2+ was shown to affect the pattern of changes in tryptophan fluorescence anisotropy and birefringence of actin filament at the moment of HMM interaction with actin, providing HMM contains DTNB light chains. Anisotropy of tryptophan fluorescence and birefringence of actin filaments rises in the absence of Ca2+ (pCa greater than or equal to 7), while in its presence (pCa less than or equal to 6) these values drop down. Furthermore, these changes become cooperative when Ca2+ concentration increases from pCa = 7 to pCa = 6. It was shown that the binding of HMM devoid of DTNB light chains to F-actin decreases tryptophan fluorescence anisotropy and birefrigence of actin filaments, regardless of Ca2+ concentration. Ca2+-dependent structural changes of F-actin induced by interaction of heads of myosin molecules with actin are assumed to be of great importance in regulation of muscle contraction of vertebrate skeletal muscles.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005454:Fluorescence Polarization; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D008860:Microscopy, Ultraviolet; D009119:Muscle Contraction / Q000187:drug effects; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D015879:Myosin Subfragments / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D011817:Rabbits
D000199:Actins; D009124:Muscle Proteins; D015879:Myosin Subfragments; D002118:Calcium
Izuchenie strukturnykh izmeneniĭ sokratitel'nykh belkov myshechnogo volokna s pomoshch'iu poliarizatsionnoĭ ul'trafioletovoĭ fluorestsentnoĭ mikroskopii. VII. Vliianie Ca2+ na kharakter konformatsionnykh izmeneniĭ F-aktina, indutsirovannykh sviazyvaniem tiazhelogo meromiozina.