Rapid identification of correctly located intracerebroventricular cannula in the rat.
We describe a radiographic technique to identify a correctly located cannula in the ventricular system of the rat brain during life. A suspension of thorium dioxide was infused into the left lateral ventricle through a cannula placed in the brain under stereotaxic guidance. Correctly located injections were identified by the presence of a distinct thorium shadow on lateral X-ray of the in situ brain. When 6-hydroxydopamine was co-injected with thorium the resultant depletion of striatal dopamine and the changes in a conditioned avoidance responses were correlated with the degree of spreading of thorium through the ventricular system.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D002404:Catheterization; D014695:Cerebral Ventriculography / Q000379:methods; D003342:Corpus Striatum / Q000032:analysis*; D004298:Dopamine / Q000032:analysis; D006892:Hydroxydopamines / Q000008:administration & dosage*; D007031:Hypothalamus / Q000032:analysis*; D007276:Injections, Intraventricular / Q000592:standards*; D008297:Male; D009638:Norepinephrine / Q000032:analysis; D016627:Oxidopamine; D051381:Rats; D011919:Rats, Inbred Strains; D013911:Thorium Dioxide / Q000008:administration & dosage*
D006892:Hydroxydopamines; D015102:3,4-Dihydroxyphenylacetic Acid; D016627:Oxidopamine; D013911:Thorium Dioxide; D004298:Dopamine; D009638:Norepinephrine