Microautoradiographic study on the tissue localization of liposome-entrapped or unentrapped 3H-labeled beta-galactosidase injected into rats.
The localization of intravenously injected liposome-entrapped or unentrapped 3H- beta-ga lactosidase in various tissues of rats was investigated by microautoradiography. The microautoradiographic silver grains, indicating the uptake of both forms of the enzyme were observed in all of the rat tissues studied, such as the liver, kidneys, spleen, lungs, heart, muscle and brain. The liver was found to be the most active in taking up both forms of the enzyme and probably Kupffer cells were primarily involved in the uptake of the enzyme. Also in the brain, the uptake of both forms of the enzyme was observed, though far less extensively, through the blood-brain barrier. The uptake of the enzyme in the brain was somewhat greater in the gray matter than in the white matter.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000201:enzymology; D005696:Galactosidases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008081:Liposomes / Q000008:administration & dosage; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism*
D008081:Liposomes; D005696:Galactosidases; D001616:beta-Galactosidase