Purification and characterization of excretory and secretory antigen of Toxocara canis larvae.
Excretory/secretory (ES) antigen of Toxocara canis larvae was isolated from the culture medium of second-stage larvae by gel filtration. The antigen induced production of not only IgM or IgG but also IgE antibody. The antigen, when treated with guanidine hydrochloride and 2-mercaptoethanol, lost its allergenic activity but retained an ability to induce IgM or IgG antibody. Purified antigen was of glycoprotein nature and had a molecular weight of 35,000. The whole antigen showed a cross-reaction with the serum from Ascaris suum-infected mice. The antigen did not contain phosphorylcholine as a structural component.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007814:Larva / Q000276:immunology; D008297:Male; D051379:Mice; D010767:Phosphorylcholine / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D014119:Toxocara / Q000276:immunology*; D014120:Toxocariasis / Q000276:immunology
D000941:Antigens; D010767:Phosphorylcholine