Lipoxygenase from rabbit reticulocytes: iron content, amino acid composition and C-terminal heterogeneity.
Purified lipoxygenase from rabbit reticulocytes contains 1 g atom iron per molecule. The amino acid composition shows that the enzyme contains 11 cysteine residues but no disulfide bridges and contains a relatively high content of hydrophobic amino acids. The enzyme shows heterogeneity in the C-terminus. With the tritium labeling method the amino acids His, Asn and Ile were found in the C-terminal region. The absorption co-efficient was found to be 1.68 (E0.1% 280 1 cm) in the native state and 1.77 in the unfolded state.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D007501:Iron / Q000032:analysis; D008084:Lipoxygenase / Q000097:blood*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D013438:Sulfhydryl Compounds / Q000032:analysis
D000596:Amino Acids; D013438:Sulfhydryl Compounds; D007501:Iron; D008084:Lipoxygenase