[Demonstration and partial purification of a convulsant from Cnestis glabra (Connaracea): effect on cells in culture].
A dialysable and thermostable compound was partially purified from Cnestis glabra (Connaracea) seeds. It is insoluble in organic solvents. At a dose corresponding to 0.5 g of seeds it has a lethal effect on mice after convulsive attacks. Sodium dipropylacetate, an anticonvulsive agent prevents the convulsions but not the death of the animals. The toxic compound inhibits protein synthesis in a rabbit reticulocyte lysate and in hepatoma tissue culture; moreover in the cell culture, the incorporation of thymidine into DNA is inhibited.
D000818:Animals; D002460:Cell Line; D003292:Convulsants / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D004261:DNA Replication / Q000187:drug effects; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D010944:Plants / Q000032:analysis*; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D012639:Seeds; D014635:Valproic Acid / Q000494:pharmacology
D003292:Convulsants; D014635:Valproic Acid
Mise en évidence et purification partielle d'un convulsivant à partir de Cnestis glabra (Connaracées): effet sur des cellules en culture.