[Morphofunctional characteristics of peritoneal macrophages in mice after cryopreservation].
Peculiarities of changes in morphology of various cells of peritoneal mouse exudate after cryopreservation are described. It is found that in monocytic-macrophage suspension the segment-nuclear neutrophils, reticular and mast cells are the most cryolable, small lymphocytes and monocytes--cryostable. It is demonstrated that after cryotreatment the adhesive activity of the cells and their absorptivity decrease, the pattern of EAC-rosette formation being changed. A method is described of cell restoration through their incubation after cryopreservation for 30 min at 37 degrees C in the medium with serum addition.
D000818:Animals; D001202:Ascitic Fluid / Q000503:physiopathology*; D002452:Cell Count; D003584:Cytological Techniques; D005615:Freezing; D008264:Macrophages / Q000502:physiology / Q000648:ultrastructure*; D051379:Mice; D008808:Mice, Inbred CBA; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000502:physiology / Q000648:ultrastructure; D010587:Phagocytosis
Morfo-funktsional'naia kharakteristika peritoneal'nykh makrofagov mysheĭ posle kriokonservatsii.