[Intramolecular dynamics and electron transfer in photosynthetic reaction centers. A study using luminescence].
The temperature dependences of fluorescence and phosphorescence spectra maxima of chromophor labels--endogenic (tryptophan) and exogenic (eosinisothiocyanate)--were measured for the preparations of photosynthetic membranes and reaction centers from Rhodospirillum rubrum. It was found that the dipole mobility of protein-lipid matrix in the vicinity of the chromophores intensified markedly with a temperature rise from 150 to 300K resulting in the corresponding relaxation time tau r decrease from 10(0) to 10(-8) s. The efficiency of direct transfer of the photomobilized electron in the system of quinone acceptors (A1- leads to A2) of reaction centers (characteristic half-times of the process being 10(-3) divided by 10(-4) s) was shown also to increase sharply at temperatures higher than 200K parallel to the enhancement of molecular motions with tau r approximately 10(-8) s. Meanwhile, changes observed in the rate of recombination of primary photoproducts, i.e. an oxidized bacteriochlorophyll dimer, P+ and a reduced acceptor, A1- (characteristic half-time of 10(-1) divided by 10(-2) s) and the activization of low-frequency motions with tau r approximately 10(-3) s in the external layers and tau r less than 1 s in the internal parts of the reaction centers protein develop over the same range of low temperatures (150-220 K). The nature of interactions which determine the dependence of the photosynthetic electron transport on the molecular mobility of the membrane proteins is discussed.
D001426:Bacterial Proteins / Q000378:metabolism*; D004579:Electron Transport; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes; D008163:Luminescent Measurements; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins; D012247:Rhodospirillum rubrum / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins
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