Glycogen synthesis from serine in isolated perfused hindquarters of acutely uraemic rats.
Isolated hindquarters of bilaterally nephrectomized and sham-operated rats were perfused in the presence and absence of 14C-labelled serine, respectively. After a perfusion period of 30 min 14C-serine was 4,074 +/- 270 dpm/ml in the perfusion medium of sham-operated animals and decreased to 2,800 +/- 190 dpm/ml in the medium of acutely uraemic rats. Muscle glycogen concentration in sham-operated animals was 1.10 +/- 0.04 mg/g wet weight in the absence and 1.03 +/- 0.11 mg/g in the presence of serine. In contrast, in acutely uraemic rats there was a glycogen concentration of 0.57 +/- 0.09 mg/g in the absence of serine. Glycogen was increased in the presence of serine in the perfusion medium, the value being 1.50 +/- 0.13 mg glycogen/g wet weight. Incorporation of labelled serine into skeletal muscle glycogen was significantly higher in acutely uraemic animals (15 +/- 0.5 mumol/g glycogen) than in sham-operated animals (10 +/- 0.4 mumol/g). The results are compatible with the hypothesis that serine increases muscle glycogen synthesis in acute uraemia.
D000208:Acute Disease; D000818:Animals; D005260:Female; D006003:Glycogen / Q000096:biosynthesis*; D006614:Hindlimb; D009132:Muscles / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000378:metabolism*; D014511:Uremia / Q000378:metabolism*
D012694:Serine; D006003:Glycogen