The transient kinetics of uptake of galactosides into Escherichia coli.
The uptake of galactosides into Escherichia coli via the lactose permease was studied in the time range 0.01-10s by rapid mixing and quenched flow. An initial transient was observed under two conditions. Firstly, a lag in the approach to the steady state was observed at low galactoside concentrations (less than Km). Secondly, a burst of uptake was observed when anaerobic cell suspensions were mixed with aerobic substrate solutions. However, the cause of the burst of uptake appears to be a burst in the rate of respiration. The rate of galactoside uptake during this phase is 10-fold greater than during the steady state.
D000963:Antimetabolites / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005697:Galactosides / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D009598:Nitrophenylgalactosides / Q000378:metabolism; D013862:Thiogalactosides / Q000494:pharmacology
D000963:Antimetabolites; D005697:Galactosides; D006027:Glycosides; D007476:Ionophores; D013862:Thiogalactosides; D009598:Nitrophenylgalactosides; C031803:thiodigalactoside