Rapid stopping of A23187 action by phosphatidylcholine.
The action on pigeon erythrocytes of the Ca2+ ionophore, A23187, can be nearly completely stopped within 30 s by treatment with a phosphatidylcholine dispersion at 39 degrees C. A time-limited 45Ca2+ uptake pulse can be produced by treating cells sequentially with A23187 and lipid, and the time-course of expulsion of this 45Ca2+ uptake pulse can be easily determined.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors*; D000001:Calcimycin / Q000037:antagonists & inhibitors*; D002118:Calcium / Q000097:blood; D010856:Columbidae; D004533:Egtazic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D008051:Lipid Bilayers / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D000001:Calcimycin; D004533:Egtazic Acid; D002118:Calcium