[Directed isolation of gram-negative asporogenous bacteria from natural substrates].
A method for selective isolation of gramnegative nonsporulating bacteria from soil substrates was developed. The method consists of plating out the substrates on a glucose-yeast medium with addition of benzylpenicillin and nalidixic acid. The isolation frequency of gramnegative nonsporulating bacteria increased under such conditions from 9-16 (control) to 80-100 per cent. At the same time the isolation frequency of gram-positive bacteria decreased from 88.5 to 9.6 per cent.
D001431:Bacteriological Techniques; D003470:Culture Media / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000302:isolation & purification; D008034:Lincomycin / Q000494:pharmacology; D009268:Nalidixic Acid / Q000494:pharmacology; D010400:Penicillin G / Q000494:pharmacology; D012988:Soil Microbiology
D003470:Culture Media; D009268:Nalidixic Acid; D008034:Lincomycin; D010400:Penicillin G
Napravlennoe vydelenie iz prirodnykh substratov gramotritsatel'nykh nesporoobrazuiushchikh bakteriĭ.