Immunochemistry of an allotypic low density lipoprotein in man.
This paper presents data on a human allotypic polymorphism carried on a low density lipoprotein (As-LDL). The As polymorphism was investigated by a rabbit antiserum which was firstly used to analyze the serum level and the isoelectric point of the As lipoprotein in sera of different phenotype. This antiserum was also used to purify As-LDL by immunochromatography. Afterwards the peptidic maps of the APO proteins from different As-LDL were compared. While no correlation did result between the serum level and the serological phenotype of the As lipoprotein, the peptidic distribution of the APO parts from different As-LDL showed differences correlated to the allotypic phenotype.
D000818:Animals; D001053:Apolipoproteins / Q000032:analysis; D002846:Chromatography, Affinity; D006801:Humans; D007106:Immune Sera; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D008077:Lipoproteins, LDL / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D010449:Peptide Mapping; D010641:Phenotype; D011110:Polymorphism, Genetic; D011817:Rabbits; D013789:Thalassemia / Q000097:blood / Q000235:genetics
D001053:Apolipoproteins; D007106:Immune Sera; D008077:Lipoproteins, LDL