Blood acetaldehyde levels in alcohol-dosed rats after treatment with ANIT, ANTU, dithiocarbamate derivatives, or cyanamide.
In adult female Wistar rats, pretreated by gavage with two doses - 16 or 256 mumol/kg - of cyanamide, TMTD (tetramethylthiuram disulfide), TMTM (tetramethylthiuram monosulfide), Ziram or Zineb at 90 min or 18 h before administration of 2 g of ethanol/kg i.p., the blood acetaldehyde levels were significantly increased for 90 - 240 min after ethanol administration (exceptions were noted after exposure to Zineb for 90 min or to low-dosed cyanamide for 18 h). After pretreatment for identical periods with ANTU (N-1-naphthylthiourea) or ANIT (1-naphthylisothiocyanate) at doses extending into the LD50 range, the blood acetaldehyde levels of rats given the same dose of ethanol remained uninfluenced. The increase in blood acetaldehyde recorded after 16 mumol/kg p.o. of TMTM and TMTD remained detectable for up to 48 h. Onset of the cyanamide action occurred already after 45 min. While recognizing that results from animal experiments cannot be transposed without restriction to the human situation, it is concluded that occupational contacts with ANTU or ANIT are not likely to elicit increased blood acetaldehyde levels in man after ingestion of alcohol. The risk of an ethanol intolerance reaction due to a rise in blood acetaldehyde therefore does not appear to be warranted. The present findings indicate, however, that exposure to TMTD, TMTM, Ziram, Zineb or cyanamide is associated with a definite health risk; because of the long persistence of these substances in the body, the risk exists for a long time post-exposure.
D015058:1-Naphthylisothiocyanate / Q000494:pharmacology*; D000079:Acetaldehyde / Q000096:biosynthesis / Q000097:blood*; D000818:Animals; D003484:Cyanamide / Q000494:pharmacology*; D003486:Cyanides / Q000494:pharmacology*; D004347:Drug Interactions; D000431:Ethanol / Q000378:metabolism*; D005260:Female; D051381:Rats; D011919:Rats, Inbred Strains; D013859:Thiocarbamates / Q000494:pharmacology*; D013861:Thiocyanates / Q000494:pharmacology*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology; D013893:Thiram / Q000031:analogs & derivatives / Q000494:pharmacology*; D015038:Zineb / Q000494:pharmacology; D015039:Ziram / Q000494:pharmacology
D003486:Cyanides; D013859:Thiocarbamates; D013861:Thiocyanates; C034033:tetramethylthiuram monosulfide; D013893:Thiram; D000431:Ethanol; D003484:Cyanamide; D015058:1-Naphthylisothiocyanate; D015039:Ziram; D000079:Acetaldehyde; D013890:Thiourea; C005277:alpha-naphthyl thiourea; D015038:Zineb