Initial characterization of the ferric iron transport system of Corynebacterium diphtheriae.
Transport of ferric iron into Corynebacterium diphtheriae C7(beta) was shown to occur by a high-affinity, active transport system. Optimal rates were at pH 6.8 and 40 degrees C. Strong inhibition of uptake by carbonyl cyanide m-chlorophenylhydrazone was consistent with the electrochemical proton gradient as the major energy source for iron transport, and inhibition by Hg2+ indicated that sulfhydryl groups were also important. Evidence was obtained for stimulation of iron uptake at pH 8.0 by a dialyzable, extracellular factor present in conditioned medium from low-iron cultures of C. diphtheriae.
D001693:Biological Transport, Active / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D003353:Corynebacterium diphtheriae / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology; D013696:Temperature
D002412:Cations; D005290:Ferric Compounds; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D007501:Iron; D008628:Mercury