Monoclonal antibodies specific for glial and neuronal antigens in the young rat cerebellum.
Monoclonal antibodies from 15 different hybridomas derived from the fusion of mouse spleen cells with a myeloma cell line were selected. Mice were immunized with the particulate fraction from 10-13-day-old rat cerebella. Hybridoma secreting antibodies were screened simultaneously by both immunocytochemistry and binding assay. Each antibody reacts with specific cerebellar neuronal or glial cells and structures.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D002531:Cerebellum / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006825:Hybridomas / Q000276:immunology; D007120:Immunochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000276:immunology*; D009474:Neurons / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D000941:Antigens