Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells.
A monoclonal antibody, Ki-M1, was produced, and its immunoreactivity was tested by light and electron-microscopic immunohistochemistry. Ki-M1 was found to react with monocytes, cells of the mononuclear phagocyte system (MPS), interdigitating reticulum cells (IDC), and the so-called indeterminate dendritic cells of lymphoid tissue. No reactivity was seen in other human tissues or other hematopoietic cells, including granulocytes and cells of the unstimulated promyelocyte cell line HL-60. Thus, Ki-M1 is the first of the monoclonal antibodies to MPS cells to react with both human IDC and MPS cells. This suggests that IDC and MPS cells may have a common cytogenesis.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000938:Antigen-Presenting Cells / Q000201:enzymology / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002460:Cell Line; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000648:ultrastructure; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000201:enzymology / Q000276:immunology* / Q000648:ultrastructure; D014066:Palatine Tonsil / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface