Proflavine binding to poly(rC-rA) inverts the CD spectrum but not the helix handedness.
The interaction of proflavine hemisulfate with the sodium salt of poly(rC-rA) in solution (unbuffered) yields an inverted (mirror-like) circular dichroism (CD) spectrum to that of the free poly(rC-rA). Simultaneously, an induced negative Cotton effect appears in the proflavine band region with a maximum at 467 nm and a slight shoulder at 420 nm. This observation may be explained as resulting from the formation of a poly(rC-rA).proflavine complex with the polynucleotide existing as a right-handed parallel chain duplex with the proflavine intercalated between the CpA sequence and not the ApC sequence. The intercalation geometry here is expected to be analogous to that found in the crystal structure of the dinucleotide CpA.proflavine complex (Westhof et al. J. Mol. Biol., 1981) which forms a miniature right-handed helix. Although normally an inverted spectra could be attributed to a reversal in the helix handedness, the similarity in the 31P nuclear magnetic resonance spectra between the free and proflavine bound poly(rC-rA) indicates that their handedness is the same. The inverted CD spectrum may be a result of the different stacking orientation between the intercalated proflavine and the A-A base-pair on one hand and the triply hydrogen bonded protonated C-C base-pair on the other.
D000166:Acridines; D001482:Base Composition; D001665:Binding Sites; D002942:Circular Dichroism; D007364:Intercalating Agents; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011061:Poly A; D011066:Poly C; D011131:Polyribonucleotides; D011370:Proflavine
D000166:Acridines; D007364:Intercalating Agents; D011131:Polyribonucleotides; D011061:Poly A; C023794:poly(A-C); D011066:Poly C; D011370:Proflavine