Some considerations of methodology and standardization of apolipoprotein A-I immunoassays.
Apolipoprotein A-I (apo A-I) is the major protein of high-density lipoprotein. Compelling evidence suggests that measurement of concentrations of apo A-I in serum may be useful in predicting and assessing ischemic heart disease. The following review of methods of isolation, characterization, and assay of apo A-I has been developed to assess the possibility of standardizing apo A-I immunoassays. We consider the properties of apo A-I that have an effect on its quantification, such as self-association, polymorphism, and stability characteristics. We attempt to review critically the various methods presented, but more information about the physicochemical properties of the protein is required before definitive recommendations can be made.
D000328:Adult; D000917:Antibody Formation; D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood* / Q000302:isolation & purification / Q000592:standards; D002850:Chromatography, Gel; D003327:Coronary Disease / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D011863:Radioimmunoassay / Q000379:methods; D014461:Ultracentrifugation
D016632:Apolipoprotein A-I; D001053:Apolipoproteins