Three closely linked latent rabbit IgG allotype gene products: a1, d12, e14.
Rabbit latent allotypes in the serum are non-allelic immunoglobulin markers that are transiently expressed in low concentration. In this report we describe the isolation and characterization of a linked series of latent allotypes on the gamma-chain which were found in two rabbits. This latent allogroup contains the determinants a1, d12, e14, which corresponds to the known nominal haplotype I. The latent allogroup is only periodically expressed in the serum and transmitted in a non-Mendelian fashion. However, although only intermittently expressed, both a1 and e14 were found to be displayed in a polyclonal manner by isoelectric focusing. The a1 was associated with the Fab region, the d12 was associated with the hinge region, and the e14 was associated with the Fc region when the peptides were assayed after enzymatic cleavage. The non-allelic demonstration of these genetic markers indicates that each of the rabbits studied carries all the alternative genes for the major three allotypic loci in the gamma-chain. It is possible that other rabbits bear them as well, but they remain "silent" at any given time.
D000818:Animals; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D007126:Immunoglobulin Allotypes / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000235:genetics* / Q000378:metabolism; D007525:Isoelectric Focusing; D008297:Male; D010206:Papain / Q000494:pharmacology; D010434:Pepsin A / Q000494:pharmacology; D014176:Protein Biosynthesis; D011817:Rabbits; D011863:Radioimmunoassay
D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D010206:Papain; D010434:Pepsin A