[Laboratory procedures with adenoviruses. X. Hemagglutination with glutaraldehyde-fixed erythrocytes].
Erythrocyte preparations from man, rat, and monkey, fixed by glutaraldehyde (0.06%, final concentration, 30 min room temperature), remained agglutinable by various human adenoviruses. While incomplete adenovirus hemagglutinins from species of subgenera A, C, and E did not react with fixed blood cells, the numerous species of subgenera B and D, in which hemagglutination and hemagglutination-inhibition are essential for identification, did so. Fixed blood cells were also agglutinable by indirect hemagglutinins of adenovirus 9 and 11; hence, hemagglutination-enhancement tests with fixed blood cells are feasible. Fixed blood cells remained stable and agglutinable up to one year; they could also be lyophilized.
D000260:Adenoviruses, Human / Q000145:classification / Q000276:immunology*; D000818:Animals; D004912:Erythrocytes; D005404:Fixatives; D005612:Freeze Drying; D005976:Glutaral; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006386:Hemagglutination Tests / Q000379:methods*; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D008253:Macaca mulatta; D051381:Rats; D011919:Rats, Inbred Strains
D005404:Fixatives; D006389:Hemagglutinins, Viral; D005976:Glutaral
Laboratoriumspraxis bei Adenoviren. X. Hämagglutination mit glutaraldehydfixierten Erythrozyten.