99Mo metabolism in sheep after the intravenous injection of [99Mo] thiomolybdates.
[99Mo]di-, tri-, and tetrathiomolybdate (5.4 to 62.5 mg of Mo) were given by intravenous injection to sheep maintained on a sulfur-supplemented (3 g of S/kg) diet. All the compounds were metabolized very rapidly over the first 15 min postinjection, but relatively slowly thereafter, with a t1/2 of about 30 hr for dithiomolybdate and 40 h for tri- and tetrathiomolybdate. The [99Mo] metabolites present in plasma were identified by Sephadex G-25 chromatography. The main fate of the compounds injected appeared to be stepwise transformation to molybdate and subsequent rapid urinary elimination. Over 90-100 hr more than 90% of the radioactivity was excreted in urine, compared to less than 5% in the feces. The trichloroacetic acid (TCA) solubility of plasma copper and the diamine oxidase activity of ceruloplasmin was depressed, particularly over the first 15 min postinjection and a more persistant TCA-insoluble Cu fraction was apparent.
D000818:Animals; D004492:Edetic Acid / Q000378:metabolism; D007275:Injections, Intravenous; D008297:Male; D008982:Molybdenum / Q000378:metabolism*; D010084:Oxidation-Reduction; D011868:Radioisotopes; D012756:Sheep; D013997:Time Factors
D011868:Radioisotopes; C034539:dithiomolybdic acid; C034540:trithiomolybdic acid; D008982:Molybdenum; C020809:tetrathiomolybdate; D004492:Edetic Acid