Chloramphenicol-inducible gene expression in Bacillus subtilis.
A cloned Bacillus pumilus cat gene expresses chloramphenicol-inducible chloramphenicol acetyltransferase activity in Bacillus subtilis. The chloramphenicol inducibility trait was shown to be determined by a 234-bp region of the cloned DNA. Nucleotide sequence analysis of this 234-bp segment indicated that the cat ribosome-binding site occurs within a 40-bp region containing 14-bp terminal inverted repeat sequences. Transcription of this region into RNA should sequester the cat ribosome-binding site in a stable stem-loop conformation. Chloramphenicol-mediated destabilization of the stem-loop is suggested as the basis for the chloramphenicol inducibility phenotype.
D000123:Acetyltransferases / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology*; D015500:Chloramphenicol O-Acetyltransferase; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics; D013045:Species Specificity
D012333:RNA, Messenger; D002701:Chloramphenicol; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase