Solid-phase immunoassays using chimera antibodies prepared with monoclonal or polyclonal anti-enzyme and anti-erythrocyte antibodies.
The possibility of using covalently prepared chimera antibodies in solid phase immunoassays was tested. The antibody specific for the antigen to be quantified was conjugated with a polyclonal or monoclonal antibody specific for an enzyme or for erythrocytes. Using these conjugates, labelling was done by enzyme/anti-enzyme or erythrocyte/anti-erythrocyte interactions. The chimera antibodies were used for quantitation of human IgE and for titration of human anti-Echinococcus granulosus antibody by solid-phase immunoassays, and were found to possess equal or higher amplification than conventionally prepared enzyme-antibody conjugates. A washing device for erythroadsorption test is also described. It allows such a test to be expressed in quantitative terms, as is the case in enzyme-immunoassays.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002678:Chimera; D004443:Echinococcosis / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005696:Galactosidases / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis; D007518:Isoantibodies / Q000276:immunology*; D051379:Mice; D010544:Peroxidases / Q000276:immunology*; D011817:Rabbits; D001616:beta-Galactosidase / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007518:Isoantibodies; D007073:Immunoglobulin E; D006735:Horseradish Peroxidase; D010544:Peroxidases; D005696:Galactosidases; D001616:beta-Galactosidase