Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.
Alkali-treated lipopolysaccharide from Brucella abortus S1119.3 coupled to agarose beads by cyanogen bromide activation resulted in an immunoadsorbent with which a large amount of B. abortus-specific antibodies could be purified. The method described gave alkali-treated lipopolysaccharide binding efficiencies of up to 98%. There was little loss of alkali-treated lipopolysaccharide from the column after several pH shifts, allowing the immunoadsorbent to be regenerated and used repeatedly.
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D008070:Lipopolysaccharides / Q000276:immunology*
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides