Harding-passey mouse-melanoma tyrosinase inactivation by reaction products and activation by L-epinephrine.
1. Harding-Passey mouse-melanoma tyrosinase (EC 1.14.18.1) is inhibited during L-3,4-dihydroxyphenylalanine oxidation by reaction products. L-3,4-dihydroxyphenyl 3-[14C]alanine oxidation products bind to the enzyme, as demonstrated by gel electrophoresis and radioactivity measurements. 2. The enzyme interacts with indoles and oxidizes dopamine and norepinephrine. 3. L-epinephrine activates tyrosinase at non hormonal concentrations and bovine serum albumin protects the enzyme from auto-inhibition. 4. The inhibition of the Harding-Passey mouse-melanoma tyrosinase, during substrate oxidation, is very similar to that of mushroom enzyme.
D000818:Animals; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors*; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004789:Enzyme Activation; D004837:Epinephrine / Q000494:pharmacology*; D008543:Melanins / Q000502:physiology; D008545:Melanoma / Q000201:enzymology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000201:enzymology; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D008543:Melanins; D012710:Serum Albumin, Bovine; D004295:Dihydroxyphenylalanine; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D004837:Epinephrine