[Carcinogen screening in mammalian cells in vitro. The clastogenicity and the SCE test].
A survey is given about problems related to cheking for genotoxicity of environmental chemicals. Cytogenetic effects, induction of structural chromosome aberrations (clastogenicity) and sister chromatid exchanges (SCEs), are very sensitive indicators of genetic damage in eukaryotic cells. Problems and methods of the clastogenicity and SCE assay in mammalian cells in vitro are described. These bioassays are correlation tests and do not allow a definitive prediction of carcinogenic activity of chemicals, but they are a valuable tool for the evaluation of genetic/carcinogenic risks. Problems of extrapolation to man are discussed.
D000818:Animals; D002273:Carcinogens; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004353:Drug Evaluation, Preclinical; D008322:Mammals; D009152:Mutagenicity Tests / Q000379:methods*; D012854:Sister Chromatid Exchange
D002273:Carcinogens
Kanzerogenscreening in Säugetierzellen in vitro. Der Klastogenitäts- und der SCE-Test.