Escherichia coli ribosomes translate in vivo with variable rate.
The question of whether or not 'rare' codons are translated with the same rate as 'common' codons was investigated by measuring the translation time for two genes, lacI and bla, rich in rare codons, and comparing the results with the translation times measured on fus, tsf, tuf and rpsA which have very few rare codons. The rate of synthesis of the lac repressor was first measured with the up-promoter mutation lacIq1 present on the high copy number plasmid pBR322. In such a strain the average translation times for lacI and bla were 50% slower than the rate calculated from the translation time for the four ribosomal proteins. In a strain having lacIq1 on an F'lac episome this difference was much smaller, thus slow translation of genes rich in rare codons is exaggerated in strains with increased drain on the rare codon tRNAs. The data do not exclude that only a subset of the rare codons is translated more slowly. Translation times were also measured in cells growing in different media, and the translation chain growth rate was found to increase by approximately 40% going from acetate medium to a fully supplemented medium.
D003062:Codon; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D010445:Peptide Elongation Factors / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013045:Species Specificity
D003062:Codon; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; C015494:ribosomal protein S1