Lipid synthesis in isolated rat hepatocytes: activation by insulin and vanadate and inhibition by ouabain.
Lipid synthesis from [1-14C] acetate was stimulated by insulin in rat hepatocytes. Vanadate also enhanced lipid synthesis, although its effect was about 2-3 times higher than that of insulin, whereas ouabain significantly decreased hepatic lipid synthesis. The stimulatory effects of both insulin and vanadate were not dependent on extracellular Ca2+. Also, this stimulation was not mimicked by either ouabain or ouabain plus vanadate, even though they are expected to induce a non-specific increase of cytoplasmic Ca2+ concentration. These results suggest that insulin and vanadate stimulation of lipid synthesis may not be mediated by changes in Ca2+ fluxes through plasma membrane.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000096:biosynthesis; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D000085:Acetates; D002250:Carbon Radioisotopes; D007328:Insulin; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D014639:Vanadium; D014638:Vanadates; D010042:Ouabain; D019342:Acetic Acid