The lipoprotein profile and phospholipase activity of subretinal fluid.
We have studied the lipoprotein profile of subretinal fluid by crossed immunoelectrophoresis using an antiserum against apolipoprotein A-I. If the detachment had occurred recently, we found that the subretinal fluid contained only the alpha 1-lipoprotein of serum. However, if the detachment was of longer duration an increasing amount of an electrophoretically slower-moving lipoprotein was also present in the subretinal fluid. We present evidence that subretinal fluid contains an enzyme, phospholipase A, which can form the slower-moving component from alpha 1-lipoprotein. Since the retina has a high phospholipid content, permanent damage may be caused to its structure by the action of this hydrolysing enzyme.
D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008074:Lipoproteins / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012163:Retinal Detachment / Q000378:metabolism*
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D008074:Lipoproteins; D010740:Phospholipases; D010741:Phospholipases A