Transfection of Bacillus subtilis protoplasts by bacteriophage phi do7 DNA.
DNA from the Bacillus subtilis temperate bacteriophage phi do7 was found to efficiently transfect B. subtilis protoplasts; protoplast transfection was more efficient than competent cell transfection by a magnitude of 10(3). Unlike competent cell transfection, protoplast transfection did not require primary recombination, suggesting that phi do7 DNA enters the protoplast as double-stranded molecules.
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D007700:Kinetics; D011523:Protoplasts / Q000502:physiology*; D014162:Transfection
D004279:DNA, Viral