Adaptation of enzyme-linked immunoassay for detection of avian and murine antibodies to Eimeria.
The enzyme-linked immunosorbent assay (ELISA) was adapted to detect antibodies to Eimeria. Avian serum antibodies were easily demonstrated and were comparable in titer to indirect fluorescent-antibody (IFA) test levels reported by others. Hybridoma-produced murine antibodies were also detected by ELISA.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis; D000953:Antigens, Protozoan / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D004539:Eimeria / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan