The nucleotide sequence of 8 S RNA bound to preribosomal RNA of Novikoff hepatoma. The 5'-end of 8 S RNA is 5.8 S RNA.
8 S RNA of Novikoff hepatoma was characterized by fingerprinting, sequencing gels, and by hybridization to rat ribosomal DNA clones. The data obtained show that 8 S RNA is 273 or 274 nucleotides long; ribosomal 5.8 S RNA is its 5'-terminal 156 nucleotides. All the post-transcriptional modifications found in 5.8 S rRNA were also found in 8 S RNA; no other modifications were found. The 3'-terminal 118 nucleotides were consistent with the adjoining internal transcribed spacer sequence in rDNA (Subrahmanyam, C. S., Cassidy, B., Busch, H., and Rothblum, L. (1982) Nucleic Acids Res. 10, 3667-3680). Based on its nucleolar localization, the finding that all the 8 S RNA is hydrogen-bonded to preribosomal RNA and its consistency in sequence to the cloned rat ribosomal DNA sequence, it appears that 8 S RNA is a relatively stable intermediate in the formation of 5.8 S rRNA from 45 S pre-rRNA. This stable intermediate RNA may be a useful substrate for studies on rRNA processing and for studies on eukaryotic rRNA-processing enzyme(s).
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004275:DNA, Ribosomal; D004722:Endoribonucleases; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004247:DNA; D004722:Endoribonucleases; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic