Determination of 6-N-trimethyllysine in urine by high-performance liquid chromatography.
A method for determination of 6-N-trimethyllysine in urine is described. Trimethyllysine and the chemically analogous 6-N-triethyllysine internal standard were isolated from aqueous samples by microcolumn ion-exclusion chromatography. The specimens were derivatized by reaction with 1-fluoro-2,4-dinitrobenzene and reaction byproducts extracted by organic solvents. The trimethyllysine and internal standard derivatives were separated easily from other sample constituents by reversed-phase paired-ion high-performance liquid chromatography with spectrophotometric detection at 405 nm. Standard curves were linear over a sample concentration range of 10-150 nmol/ml; the detection limit corresponded with 0.1 nmol trimethyllysine injected into the chromatograph. The procedure was used for determination of trimethyllysine in human urine.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004139:Dinitrofluorobenzene; D005260:Female; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D013053:Spectrophotometry
C003712:trimethyllysine; D004139:Dinitrofluorobenzene; D008239:Lysine