Preparation and properties of a crude extract toxic to guinea-pigs from Pachystigma pygmaeum, a plant causing heart failure in ruminants.
Pachystigma pygmaeum is a rubiaceous plant which causes heart failure after ingestion by ruminants. Isolation or characterization of a toxic entity has hitherto been unsuccessful. Extraction of the plant material with water or organic solvents gave poor yields of toxicity in guinea-pigs because of some unusual properties of the toxic entity. We present a method whereby liberation of a toxic entity from the plant material was achieved by fermentation with baker's yeast to give a crude extract with sufficient yield of toxicity for use in further isolation work.
D000818:Animals; D001188:Artiodactyla; D005285:Fermentation; D006168:Guinea Pigs; D006323:Heart Arrest / Q000209:etiology / Q000662:veterinary; D007928:Lethal Dose 50; D008722:Methods; D010936:Plant Extracts / Q000633:toxicity; D010939:Plant Poisoning / Q000662:veterinary; D010947:Plants, Toxic / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014118:Toxins, Biological / Q000302:isolation & purification*
D010936:Plant Extracts; D014118:Toxins, Biological