Metabolism of platelet-activating factor by blood platelets and plasma.
High performance liquid chromatography in combination with a radioactivity detector was used to study the metabolism of platelet-activating factor (1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by washed platelets, platelet-free plasma and platelet-rich plasma obtained from rabbits and humans. Degradation of platelet-activating factor to its 2-lyso derivative was observed in rabbit and human plasma. This degradation of platelet-activating factor in plasma was completely inhibited by diisopropylfluorophosphate and was partially inhibited by ethylenediamine tetraacetic acid. Washed platelets metabolized platelet-activating factor not only to the 2-lyso compound but also, by reacylation of this lyso intermediate, to an analogue of platelet-activating factor probably containing a long-chain acyl group at the sn-2 position. These transformations occurred, but to a lesser extent, in platelet-rich plasma.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007531:Isoflurophate / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000502:physiology*; D011817:Rabbits; D013045:Species Specificity
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D007531:Isoflurophate; D004492:Edetic Acid