A glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls.
A particulate enzyme preparation made from epicotyls of 1-week-old etiolated pea (Pisum sativum) seedlings was shown to incorporate glucuronic acid from UDP-D-[U-14C]glucuronic acid into a hemicellulosic polysaccharide. Optimum conditions for the incorporation include the presence of Mn2+ ions at between 4 and 10 mmol/litre and a pH between 5 and 6. UDP-D-xylose at 1 mmol/litre allows incorporation to continue for at least 8 h. In its absence, the reaction stops within 30 min. Analysis of the product by partial and total acid hydrolysis, followed by paper chromatography or electrophoresis, indicates that the polysaccharide produced is a glucuronoxylan.
D002413:Cations, Divalent / Q000494:pharmacology; D002854:Chromatography, Paper; D007887:Fabaceae / Q000201:enzymology; D005615:Freezing; D014453:Glucuronosyltransferase / Q000378:metabolism*; D010944:Plants / Q000187:drug effects / Q000201:enzymology*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000096:biosynthesis* / Q000378:metabolism; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014540:Uridine Diphosphate Xylose / Q000494:pharmacology; D014990:Xylans / Q000096:biosynthesis*
D002413:Cations, Divalent; D011134:Polysaccharides; D014990:Xylans; D014535:Uridine Diphosphate Glucuronic Acid; D014540:Uridine Diphosphate Xylose; C038910:glucuronoxylan; C007916:hemicellulose; D014453:Glucuronosyltransferase