Long-term injury in B-lymphocyte precursor cells in repeatedly-irradiated mice.
Mice irradiated with 4 doses of 4.5 Gy X-rays at 3-week intervals, demonstrated long-term proliferative defects in B lymphocytes. There was a reduced mitogenic response to bacterial polysaccharide (30%), a lower concentration (35%) of B-lymphocyte colony-forming cells (BL-CFC) in agar with an increased proportion of clusters (X 2), and a reduced concentration (30%) of plaque-forming cells. Grafts of thymocytes were able to restore the levels of BL-CFC in the short term, but in the long term large grafts of femoral marrow cells were much better in restoring the numbers of BL-CFC. The reduced mitogenesis (25%) of splenocytes by concanavalin A and the diminished number of plaque-forming cells, may suggest persistent injury in T-B cell cooperation.
D000818:Animals; D001402:B-Lymphocytes / Q000528:radiation effects*; D003114:Colony-Forming Units Assay; D006462:Hemolytic Plaque Technique; D008297:Male; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000528:radiation effects; D013997:Time Factors; D014965:X-Rays
D008934:Mitogens