Immunological phenotype related to acid alpha-naphthyl acetate esterase and acid phosphatase in childhood acute lymphoblastic leukaemia.
In 83 children with acute lymphoblastic leukaemia (ALL) the immunological phenotype of the lymphoblasts was determined using E rosetting, monoclonal anti-T cell sera, surface immunoglobulin staining and common ALL antiserum. The data were compared with acid alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (AP) cytochemical data. The vast majority of T-ALL cells proved to be ANAE-negative (30/31) and AP-positive (29/31). Null-ALL was always AP-negative (12/12), the ANAE reaction was sometimes positive (4/12). In common ALL, findings ranged from negative to positive for both enzymes. Two cases of B-ALL were ANAE-negative and AP-negative. Enzyme cytochemical determinations gave thus preferential patterns, especially for T-ALL and null-ALL. In common ALL, considerable heterogeneity was found, which may be a reflection of differences in maturation between different cases of common ALL.
D000135:Acid Phosphatase / Q000378:metabolism*; D000293:Adolescent; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002648:Child; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology* / Q000276:immunology; D009283:Naphthol AS D Esterase / Q000378:metabolism*; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology
D011947:Receptors, Antigen, B-Cell; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; D000135:Acid Phosphatase