Revelation of AB cell surface antigens on bladder-washing specimens with the immunofluorescence technique. An approach to automated cytology.
Revelation of AB cell surface antigens is possible with the immunofluorescence technique on urothelial cells from bladder irrigation fluid. Normal urothelial cells show a heterogeneous expression varying with cell type and cell viability. However, the loss of any antigenic reactivity of some cells seems to be directly related to their neoplastic features. Flow cytometry appears to be the most appropriate technique to provide the necessary objectivity for this type of study.
D000017:ABO Blood-Group System / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007507:Therapeutic Irrigation; D001743:Urinary Bladder / Q000166:cytology* / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology
D000017:ABO Blood-Group System; D000954:Antigens, Surface