Generation of a leukotriene-like substance from porcine vascular and other tissues.
When chopped porcine vascular tissue was incubated with A23187 in the presence of a cyclo-oxygenase inhibitor, a substance with the biological properties of a leukotriene was released into the supernatant. The highest concentrations of leukotriene-like material were released by coronary and pulmonary arteries and the adventitia removed from these vessels, the greatest amount being released by the adventitia. Smaller quantities of leukotriene-like substance were released from other blood vessels but not from aorta, atrial or ventricular smooth muscle. Release of the leukotriene-like material was time-dependent. The activity of the leukotriene-like substance was antagonised by FPL 55712 and its release was inhibited by BW 755c. Leukotriene-like material was also generated from guinea-pig, rat, rabbit and dog blood vessels incubated with A23187 and from guinea-pig vessels during antigen challenge.
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001158:Arteries / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D006352:Heart Ventricles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity; D011720:Pyrazoles / Q000494:pharmacology; D013189:SRS-A / Q000096:biosynthesis*; D013552:Swine
D000893:Anti-Inflammatory Agents; D011720:Pyrazoles; D013189:SRS-A; D000001:Calcimycin; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine