A common VH marker relating BALB/c alpha 1-3 dextran-binding and A/J P-azophenylarsonate-binding antibody families.
Antibodies that define the idiotypic marker 104E-MPC11 were purified from rabbit anti-M104E sera. The expression of this marker was searched for in a panel of representative myeloma and hybridoma proteins differing in antigen-binding specificities and idiotype expression. By radioimmune competition assays, the marker was distinguished from the IdX Dex idiotype of BALB/c alpha 1-3 dextran-binding proteins (alpha 1-3 Dex-BP) in that it was expressed on both IdX Dex-positive and IdX Dex-negative proteins on the one hand, and on A/J p-azophenylarsonate-binding proteins (Ar-BP) on the other. Among the later, six of eight of the proteins also expressed the major cross-reactive idiotype (CRI). The marker was expressed weakly or not at all on A/J Ar-BP, which did not express the CRI, as well as on a number myeloma and hybridoma proteins of different antigen-binding specificities, and normal IgG. The marker was localized in VH, but its full expression required the light chain, and was influenced by quaternary structure.
D000818:Animals; D000918:Antibody Specificity; D001391:Azo Compounds / Q000378:metabolism*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000235:genetics*; D003429:Cross Reactions; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011817:Rabbits; D011971:Receptors, Immunologic / Q000235:genetics*; D010132:p-Azobenzenearsonate / Q000378:metabolism*
D001391:Azo Compounds; D002352:Carrier Proteins; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D011971:Receptors, Immunologic; C036109:dextran receptor; D010132:p-Azobenzenearsonate