Automated morphometric endothelial analysis combined with video specular microscopy.
Contact and noncontact specular microscopes were tested in combination with a highly sensitive video residual-light camera and with the Kontron IPS (Image Processing System). The video information is recorded with a video recorder or with the IPS frame grabber, a digital image storage system. Examples of experimental and clinical applications are demonstrated. Nine linear and structural parameters are analyzed. Two endothelium-typical parameters are presented, which allow topological as well as hexagonal-specific geometric endothelial single-cell analysis.
D000818:Animals; D003201:Computers; D003315:Cornea / Q000473:pathology; D003316:Corneal Diseases / Q000473:pathology*; D016039:Corneal Transplantation; D004487:Edema / Q000473:pathology; D004727:Endothelium / Q000473:pathology; D005125:Eye Banks; D006801:Humans; D008853:Microscopy / Q000295:instrumentation*; D013552:Swine; D014741:Video Recording / Q000295:instrumentation*