Genetic toxicity of dopamine.
The genetic toxicity of dopamine was studied in a battery of test systems including DNA single-strand break analysis in cultured human skin fibroblasts, the Salmonella/mammalian-microsome mutagenicity test, sister-chromatid exchange analysis in human lymphocytes, the mouse-lymphoma forward mutation assay, the sex-linked recessive lethal test in Drosophila melanogaster and the micronucleus test in mouse and rat. Dopamine at concentrations of 50-300 micrograms/ml induced DNA strand breaks in human fibroblasts. It also gave a positive response in the mouse-lymphoma forward mutation assay, where a dose-dependent increase in the frequency of mutant cells was observed in the presence of dopamine, 94-750 micrograms/ml. All other tests showed no response to dopamine. The dopamine-induced DNA strand breaks in human fibroblasts were inhibited by superoxide dismutase or dithiothreitol. Furthermore, dopamine caused nicking of circular Col El DNA and bound to calf thymus DNA in vitro. It is suggested that this genetic activity of dopamine in vitro relates to oxidation of dopamine and the generation of reactive oxygen radicals, semiquinones and quinones. It is unlikely that similar reactions would occur and cause genotoxic activity of dopamine in vivo.
D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D005804:Genes, Lethal / Q000187:drug effects; D005808:Genes, Recessive / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D008223:Lymphoma / Q000503:physiopathology; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D009154:Mutation; D012854:Sister Chromatid Exchange / Q000187:drug effects; D012867:Skin / Q000378:metabolism
D009153:Mutagens; D004298:Dopamine