Bovine brain S100 proteins: separation and characterization of a new S100 protein species.
Three S100 protein species (S100a, S100b, S100a') have been purified from bovine brain using a modification of standard preparative methods. A higher yield for each protein was obtained at the last separation step. Characterization by urea/sodium dodecyl sulfate/polyacrylamide gel electrophoresis, UV absorption spectra, and fluorescence parameters provided evidence of a new tryptophan-containing S100 protein called S100a', which exhibits, as S100a and S100b, the properties of a Ca2+ binding protein.
D000818:Animals; D015415:Biomarkers; D001923:Brain Chemistry; D002118:Calcium; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005779:Immunodiffusion; D008970:Molecular Weight; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D015415:Biomarkers; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C030564:S-100 calcium-binding protein alpha subunit; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D002118:Calcium