Lysis of nitrofurantoin-resistant strain of Vibrio el tor.
Both nitrofurantoin-sensitive and nitrofurantoin-resistant strains of Vibrio el tor were found to lyze in the presence of Tris-EDTA at alkaline pH. The rate of lysis was appreciably enhanced by lysozyme. The amounts of intracellular components, viz. proteins and carbohydrates, released from the nitrofurantoin-sensitive strain by Tris-EDTA treatment, were significantly lower than those from the nitrofurantoin-resistant strain. Differences in periplasmic proteins released from Tris-EDTA treated cells of nitrofurantoin-resistant and -sensitive strains were revealed by gel electrophoresis.
D001426:Bacterial Proteins / Q000032:analysis; D001433:Bacteriolysis; D002241:Carbohydrates / Q000032:analysis; D004352:Drug Resistance, Microbial; D004492:Edetic Acid / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D009582:Nitrofurantoin / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014733:Vibrio / Q000187:drug effects / Q000502:physiology*
D001426:Bacterial Proteins; D002241:Carbohydrates; D012967:Sodium Dodecyl Sulfate; D009582:Nitrofurantoin; D004492:Edetic Acid; D009113:Muramidase