Analytical methods for quantitation of methylxanthines.
The methylxanthines can be determined in foods and biological systems by the chromatographic methods of TLC, GC, and HPLC. Ultraviolet spectroscopy following a separation procedure is also still used. More recently immunoassay methods have been developed. There is no single best method; the analyst must balance the features of each assay with the final requirements for data precision and reproducibility.
D000818:Animals; D001826:Body Fluids; D002110:Caffeine / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005504:Food Analysis / Q000379:methods; D006801:Humans; D008722:Methods; D013056:Spectrophotometry, Ultraviolet; D013805:Theobromine / Q000032:analysis*; D013806:Theophylline / Q000032:analysis*
D002110:Caffeine; D013806:Theophylline; D013805:Theobromine