Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells.
Depletion of polyamines in 9L rat brain tumor cells by treatment with alpha-difluoromethylornithine dramatically altered DNA conformation as measured by viscoelastometry. The reduction of intracellular putrescine and spermidine concentrations to less than 5 percent of their concentrations in control cells decreased the sensitivity of 9L cell DNA to x-irradiation and increased the maximum viscoelastic retardation time of the DNA. Both of these phenomena were reversed by addition of exogenous putrescine.
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D000518:Eflornithine; D008968:Molecular Conformation; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D013095:Spermidine / Q000378:metabolism; D014783:Viscosity
D004273:DNA, Neoplasm; D011073:Polyamines; D009952:Ornithine; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine