"PMN-elastase assay": enzyme immunoassay for human polymorphonuclear elastase complexed with alpha 1-proteinase inhibitor.
A solid phase, enzyme-linked immunoassay is described for the quantitative determination of the complex of human granulocyte elastase (EC 3.4.21.37) with alpha 1-proteinase inhibitor. The assay employs antibody-coated test tubes and it is suitable for routine use in clinical chemistry laboratories. Data for sample stability and test characteristics are given. A reference range of 20-180 micrograms/l elastase in plasma was determined. The diagnostic significance of granulocyte elastase levels in plasma in inflammatory diseases is discussed.
D001798:Blood Proteins / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood*; D000515:alpha 1-Antitrypsin
D001798:Blood Proteins; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase