Differences in the cochlear degeneration pattern in the guinea pig as a result of gentamicin and cis-platinum intoxication.
This study gives the results of a series of studies on inner ears carried out with the help of microdissections and surface preparations. The total degeneration patterns of the cochlea of the guinea pig due to two different ototoxic drugs were investigated with this technique. Clear differences between the results of the ototoxic drugs (gentamicin and cis-platinum) in the inner ear were observed. Gentamicin has a 'typical starting point' of degeneration on the basilar membrane. From this 'degeneration point' the loss of hair cells progressed towards the round window (fast) and the apex (slowly). The stria vascularis showed no signs of degeneration due to gentamicin. Reissner's membrane, on the other hand, showed cellular vacuolization of the endolymphatic cells. Cis-platinum (DDP) showed no 'degeneration point'; the loss of hair cells was found over the complete length of the basilar membrane, with a preference for the basilar turn. The stria vascularis, on the other hand, showed severe degenerative changes due to DDP, whereas Reissner's membrane showed no change. In using the microdissection technique we were in a position to discover these differences between the two ototoxic drugs.
D000818:Animals; D001489:Basilar Membrane / Q000473:pathology; D002945:Cisplatin / Q000009:adverse effects*; D003051:Cochlea / Q000473:pathology*; D005839:Gentamicins / Q000009:adverse effects*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000473:pathology; D006199:Hair Cells, Auditory, Inner / Q000473:pathology; D006311:Hearing Disorders / Q000139:chemically induced / Q000473:pathology; D006316:Hearing Loss, High-Frequency / Q000139:chemically induced / Q000473:pathology; D008858:Microscopy, Phase-Contrast; D012405:Round Window, Ear / Q000473:pathology; D013316:Stria Vascularis / Q000473:pathology
D005839:Gentamicins; D002945:Cisplatin