A B-cell proliferation with aberrant true E-rosette formation: a sequential study with a panel of monoclonal antibodies and other surface markers.
A case of B-CLL in prolymphocytic transformation is described in which large numbers of peripheral blood mononuclear cells possess surface immunoglobulin and rosetted with AET-treated sheep erythrocytes (SIg+E+), but lacked specific T-cell antigens. The SIg (MDGK) was shown to be an intrinsic product of the cell, whose B-cell nature was confirmed by staining with BA1, a specific anti-B-cell monoclonal antibody. The E positivity resembled the rosetting of normal T cells in certain important respects but was weak, and aberrant in certain other respects. Although E rosetting was blocked with anti-chi and anti-D but not with anti-lambda, extensive blocking studies with a panel of monoclonal antibodies indicated that this was due to steric hindrance of weak E receptor rather than specific blocking of SIg possessing anti-E activity. The case is compared and contrasted with previous rare examples of SIg+E+ proliferations and briefly discussed in relation to the recent demonstration that certain non-T cells can express E receptor in vitro.
D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D010641:Phenotype; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic