[The effect of modifiers of surface tension on the morphology and motility of boar sperm after thawing].
The comparison of the protective action of the diluent used for the freezing of boar ejaculate with the effect of the same diluent enriched with the chemical denoted as Orvus Es Paste (OEP) or Lauryl sodium sulphate (LSS) showed that semen samples with OEP had a statistically significantly higher percentage of progressively moving spermatozoa in comparison with control (P = 0.01). Sperm motility in semen samples with LSS was better than in control only after three- or six-hour exposure of thawed samples to the temperature of 38 degrees C. In comparison with the control diluent, a statistically significantly higher number of spermatozoa with intact acrosome (P = 0.01) was found in all samples with detergents; this remained true even after six hours of exposure to 38 degrees C. The evaluation of the effect of OEP and LSS indicates that after thawing the motility of spermatozoa was higher in OEP-enriched sperms (P = 0.01) whereas the numbers of spermatozoa with intact acrosome were higher (though insignificantly) is samples of semen with LSS. After six hours of exposure to 38 degrees C the differences in the evaluated criteria reached an equalized state. The diluent with twice as much yolk as in the control diluent had varying results, although the protective effect of LSS was also observed in this case.
D000818:Animals; D005615:Freezing; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000648:ultrastructure*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013552:Swine
D013501:Surface-Active Agents
Vliv modifikátorů povrchového napĕtí na morfologii a motilitu spermií kance po rozmrazení.