The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: activity and properties of UDP-glucuronyltransferase in choline-deficient rats.
The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.
D000818:Animals; D002796:Choline Deficiency / Q000201:enzymology*; D004032:Diet; D014453:Glucuronosyltransferase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000494:pharmacology
D014537:Uridine Diphosphate N-Acetylglucosamine; D014453:Glucuronosyltransferase