alpha-D-galactosyltransferase activity in calf thymus. A high-resolution 1H-NMR study.
In calf thymus an alpha-D-galactosyltransferase activity has been detected that transfers galactosyl residues from UDP-galactose to suitable acceptors having galactose at the non-reducing terminus. For example, incubation of UDP-[14C]galactose and Gal beta(1 leads to 4) GlcNAc (N-acetyllactosamine) in the presence of a calf thymus cell-free suspension containing this galactosyltransferase activity resulted in the enzymic synthesis of a 14C-labelled trisaccharide. 500-MHz 1H-NMR spectroscopic analysis revealed the structure of the trisaccharide to be: Gal alpha (1 leads to 3) Gal beta (1 leads to 4) GlcNAc. This study illustrates the suitability of the 1H-NMR method for the analysis of enzymic conversions of carbohydrate chains.
D000818:Animals; D002236:Carbohydrate Conformation; D002250:Carbon Radioisotopes; D002417:Cattle; D005700:Galactosyltransferases / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology*
D002250:Carbon Radioisotopes; D005700:Galactosyltransferases