Cultivation of arterial endothelial cells from human umbilical cord.
We have developed a simple method for the isolation of endothelial cells from human umbilical artery. The method provides a sufficient number of cells to be of experimental value. The presence of factor VIII antigen specific for endothelium has been demonstrated by immunofluorescence as well as by the peroxidase-antiperoxidase immune complex method.
D000941:Antigens / Q000032:analysis; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods; D011247:Pregnancy; D014469:Umbilical Arteries / Q000166:cytology*; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII