Quantitation of soluble immune complexes by a microplate Raji cell-ELISA method.
A Raji cell microplate-ELISA method has been devised for the detection and quantitation of putative immune complexes present in human sera. The Raji cells immobilized with glutaraldehyde (final concentration 0.67%) were conveniently utilized in the solid phase system. The specific binding of AHG diluted in PBS (without complement) increased linearly in the range of 0.1 to 5 micrograms. The purified human IgG showed some low-affinity binding which was 7-to 14-fold lower than the binding of AHG. The circulating immune complexes positive sera were found to have values above the mean of 11.95 +/- 4.09 micrograms/ml AHG equivalent for normal human sera (n = 72). The method is sensitive, reproducible and it can be performed with simplicity and rapidity.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D012995:Solubility
D000936:Antigen-Antibody Complex