The carbohydrate moiety of human platelet glycocalicin.
Glycocalicin, a predominant glycoprotein on the human platelet surface, has been purified from a platelet suspension by sonication, ammonium sulfate precipitation and acid treatment followed by chromatography on columns of wheat germ agglutinin-Sepharose and Sephacryl S-300. Ser/Thr-linked (O-linked) oligosaccharides were released by alkaline borohydride treatment, and fractionated by high performance liquid chromatography with an anion-exchange resin. The structure of a major oligosaccharide alditol separated by high performance liquid chromatography was investigated by a combination of compositional analyses, methylation and glycosidase treatments, and proposed to be a hexasaccharide alditol, NeuAc alpha 2-3Gal beta 1-4GlcNAc beta 1-6(NeuAc alpha 2-3Gal beta 1-3) N-acetylgalactosaminitol. We also found some sugar units which appeared to be intermediates in the biosynthetic pathway of the major hexasaccharide.
D001792:Blood Platelets / Q000032:analysis*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D002845:Chromatography; D006023:Glycoproteins / Q000097:blood*; D006026:Glycoside Hydrolases; D006801:Humans; D008565:Membrane Proteins / Q000097:blood*; D008745:Methylation; D008970:Molecular Weight; D009844:Oligosaccharides / Q000302:isolation & purification; D019038:Platelet Glycoprotein GPIb-IX Complex; D012694:Serine; D013912:Threonine
D002241:Carbohydrates; D006023:Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D019038:Platelet Glycoprotein GPIb-IX Complex; C023592:glycocalicin; D013912:Threonine; D012694:Serine; D006026:Glycoside Hydrolases