Detection of IgD 'hidden' lambda light chain.
Immunoelectrophoresis and immunodiffusion of some IgD myeloma proteins fail to demonstrate precipitin bands between the light chain and anti-light chain serum. Reduction and alkylation of purified IgD preparation was found to render the molecule reactive to anti-lambda serum. Iodinated IgD myeloma protein was successfully precipitated by both anti-delta and anti-lambda antibodies. Both antisera precipitated heavy and light chains of a MW of 70,000 and 24,000 daltons respectively. In addition, a band of 39,000 daltons was also evident. Reduction and alkylation of the iodinated molecule, followed by precipitation with anti-delta serum demonstrated only the 70,000 and 39,000 daltons bands. The present report indicates that by sensitive techniques, such as iodination and immunoprecipitation, non-reactive hidden light chains can be detected in IgD lambda molecules.
D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D007072:Immunoglobulin D / Q000032:analysis* / Q000302:isolation & purification; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis* / Q000652:urine; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000032:analysis / Q000276:immunology / Q000652:urine
D007072:Immunoglobulin D; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D009194:Myeloma Proteins