Immunological properties of porcine enterotoxigenic Escherichia coli heat-stable (ST) enterotoxins.
Cross-linking semipurified and purified heat-stable enterotoxin (2023 ST) to carrier proteins with glutaraldehyde pigs and rabbits were immunized. With ST antisera thus raised, a relatively simple, two-step method for isolation of ST is described. The method involved immunoadsorbent column chromatography procedure, and elution of the retained material with 6 M urea at pH 3.0 yielded purified ST with enterotoxin activity, controlled in suckling mouse test. ST purity was checked with a special staining technique in PAGE, anionic-exchange chromatography and immunodiffusion.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D001427:Bacterial Toxins; D004768:Enterotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins; D007163:Immunosorbent Techniques; D011817:Rabbits; D013552:Swine
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli)