Failure of bovine complement levels measured by radial hemolysis to correlate with tube titration.
Hemolytic complement levels in 30 duplicate samples of normal bovine sera were determined by a tube titration method (CH50) and by a radial hemolysis in gel assay. Significant correlation was not observed between the values obtained by the 2 tests. This lack of correlation could be a result of considerable error observed in values obtained for duplicate samples in the CH50 method or to the relative insensitivity of the hemolysis in gel test. The hemolytic reaction in gel was found to depend on Mg2+, thus raising questions (i) about its validity in determining total hemolytic complement or (ii) about bovine C1q depending on Mg2+ rather than Ca2+ for binding to immune complexes.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle / Q000276:immunology*; D003166:Complement Activating Enzymes / Q000032:analysis; D015922:Complement C1q; D003165:Complement System Proteins / Q000032:analysis*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D008274:Magnesium / Q000494:pharmacology
D004533:Egtazic Acid; D015922:Complement C1q; D003165:Complement System Proteins; D004492:Edetic Acid; D003166:Complement Activating Enzymes; D008274:Magnesium; D002118:Calcium