[Ultrastructural changes in Pseudomonas aeruginosa cells as affected by gentamycin in in vitro and in vivo experiments].
Morphological changes in phagocytosed cells of Pseudomonas aeruginosa under the effect of gentamicin were studied with electron microscopy using ultrathin slices. Peritoneal macrophages induced by irritation of the peritoneum with medium 199 followed by inoculation with a 1-milliard suspension of Pseudomonas aeruginosa were used. Gentamicin was administered intramuscularly in single doses of 10, 20 and 50 mg/kg. The effect of gentamicin was also studied in vitro. The in vitro studies showed that gentamicin induced rarefaction of the cytoplasm in Pseudomonas aeruginosa, elimination of the nuclear material, formation of membrane structures and separation of the cytoplasmic membrane from the cell wall. The studies with exposure of phagocytosed bacteria to gentamicin showed that the phagocytosed bacterial cells had the most pronounced changes in their ultrastructure. Under the effect of gentamicin in a dose of 50 mg/kg there appeared 2 types of the cells with changes not observed in vitro. Investigation of the digestive capacity of phagocytes demonstrated that gentamicin favoured an increase in the phagocytic activity and digestive capacity.
D000818:Animals; D001202:Ascitic Fluid / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008854:Microscopy, Electron; D010587:Phagocytosis / Q000187:drug effects; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000648:ultrastructure
D005839:Gentamicins
Ul'trastrukturnye izmeneniia v kletkakh Pseudomonas aeruginosa pod vliianiem gentamitsina v opytakh in vitro i in vivo.