Permeabilization of Bacillus subtilis to chemotaxis methyltransferase II.
Treatment of Bacillus subtilis with 0.4% (vol/vol) toluene renders cells permeable not only to small molecules but also apparently to proteins as large as 30,000 daltons. Methyl-accepting chemotaxis proteins and two smaller polypeptides were methylated when B. subtilis methyltransferase II was added to permeabilized cells.
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002630:Chemotactic Factors / Q000378:metabolism*; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D008780:Methyltransferases / Q000378:metabolism*; D014050:Toluene / Q000494:pharmacology
D001426:Bacterial Proteins; D002630:Chemotactic Factors; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D014050:Toluene; D008780:Methyltransferases; C038706:chemotaxis methyltransferase