A study of the B-A transition in DNA by gel electrophoresis.
A procedure is developed for studying the B-A transition in DNA using gel electrophoresis. The starting point has been the idea that the junction between the A and B sections, which appear within the transition interval would increase the mobility of the DNA molecules. Indeed, the mobility of DNA in a gel is shown to increase in the middle of the B-A transition due to the formation of the largest possible number of boundaries between the B and A forms. The middle of the B-A transition in supercoiled DNA appears to be shifted against the middle of the transition in open circular (as well as linear) DNA by about 1.3% towards lower ethanol concentrations under the influence of the superhelical stress.
D004247:DNA / Q000302:isolation & purification; D004278:DNA, Superhelical / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D000431:Ethanol; D009690:Nucleic Acid Conformation
D004278:DNA, Superhelical; D000431:Ethanol; D004247:DNA