Cytosol Mg2+ modulates Ca2+ ionophore induced secretion from rabbit neutrophils.
The influence of extracellular Mg2+ on Ca2+ ionophore (A23187 and ionomycin) induced secretion and changes in the cytosol pH of rabbit neutrophils suspended in Ca2+-free buffer has been investigated. While extracellular Ca2+ is obligatory for ionomycin induced secretion, we have defined conditions under which A23187 can induce secretion in Ca2+-free media. The different behaviour of these two Ca2+ ionophores is discussed on the basis of their different counter cation specificities.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology*; D008274:Magnesium / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
D004987:Ethers; D007476:Ionophores; D000001:Calcimycin; D015759:Ionomycin; D005966:Glucuronidase; D008274:Magnesium; D002118:Calcium