Hydroperoxy fatty acid formation in selenium deficient rat platelets: coupling of glutathione peroxidase to the lipoxygenase pathway.
After incubation with [1-14C]-arachidonic acid, washed platelets from selenium deficient rats produced a sevenfold greater amount of 12-hydroperoxytetraenoic acid than platelets from control animals. When stimulated with either arachidonic acid or t-butyl-hydroperoxide, antimycin-A1 treated platelets from the deficient rats also converted markedly lower amounts of [1-14C]-glucose to [14C]-CO2 than platelets from control rats. These results indicate a significant role for platelet selenium-dependent glutathione peroxidase in the enzymatic reduction of platelet-produced hydroperoxides.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000096:biosynthesis / Q000097:blood / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000097:blood*; D007700:Kinetics; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000096:biosynthesis* / Q000494:pharmacology; D008084:Lipoxygenase / Q000097:blood*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology
D001095:Arachidonic Acids; D001786:Blood Glucose; D015289:Leukotrienes; D008054:Lipid Peroxides; D016718:Arachidonic Acid; D000968:Antimycin A; C021201:12-HPETE; D005979:Glutathione Peroxidase; D008084:Lipoxygenase; D012643:Selenium