Regulation of TCGF production in T cells. II. Early membrane events after anti-Thy-1 binding by the TCGF-producing T lymphoma EL-4 G-12.
The T cell lymphoma line EL-4 G-12 produces large quantities of TCGF (IL 2) shortly after exposure to anti-Thy-1 and cross-linking reagents. Early surface events that initiate nonconstitutive synthesis and secretion have been examined by fluorescent microscopy, EM analysis, flow cytometry of anti-Thy-1, and measurements of membrane polarization. The data show that anti-Thy-1 binding leads to immediate and rapid endocytosis and shedding of the antibody molecules, but with no change in membrane potential. Cross-linking of the anti-Thy-1 results in stabilization and accumulation of the antibody on the surface, and rapid hyperpolarization. The data suggest that cross-linking of ligand antibody interactions at the surface is a regulatory event involved in initiation of the signal for TCGF synthesis and secretion.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D000961:Antilymphocyte Serum / Q000276:immunology*; D001666:Binding Sites, Antibody; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004705:Endocytosis; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006041:Goats; D007376:Interleukin-2 / Q000096:biosynthesis*; D008223:Lymphoma / Q000276:immunology*; D008564:Membrane Potentials; D051379:Mice; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013861:Thiocyanates
D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum; D003432:Cross-Linking Reagents; D005452:Fluoresceins; D007376:Interleukin-2; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate