A rapid screening technique for monoclonal antibodies with specificity for protein antigens.
A rapid technique for screening crude supernatants from antibody-producing hybridomas against protein antigen is described. The technique involves blotting of crude hybridoma supernatants to nitrocellulose filters and revealing specific binding using radiolabeled antibodies or antigen.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology; D011506:Proteins / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D007145:Immunoglobulin kappa-Chains; D011506:Proteins