Calcium-dependent association of glutathione S-transferase with the human erythrocyte membrane.
Elevations in intracellular calcium increase the adsorption of a cytoplasmic protein to human red blood cell membrane. This protein migrates on SDS polyacrylamide gels at 23,000 daltons and has been called band 8. The association of this protein with the membrane is increased in sickle cell anemia. This protein is extracted from the membrane with EGTA, a calcium chelator. Enzymatic and immunological studies identify band 8 as a glutathione S-transferase.
D000755:Anemia, Sickle Cell / Q000201:enzymology; D002118:Calcium / Q000097:blood / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D005982:Glutathione Transferase / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D012016:Reference Values
D004533:Egtazic Acid; D005982:Glutathione Transferase; D002118:Calcium