Developmental changes in human erythrocyte carbonic anhydrase levels: coordinate expression with adult hemoglobin.
In order to bolster the argument that parallel developmental changes in erythrocyte adult hemoglobin (HbA) and carbonic anhydrase (CA) content provide a potentially suitable model for the dissection of coordinate gene expression, the magnitude of fetal vs adult differences in CA I and CA II levels was examined in human red cell subpopulations obtained after varying periods of exposure to CA-dependent, NH4Cl-HCO-3-mediated, acetazolamide-modulated hemolysis. When content of CA I and CA II was immunologically assessed in cohorts surviving successively longer periods of hemolysis, cord blood red cells were divisible into two populations. Fifteen to thirty percent are rapidly disrupted and have CA I and CA II concentrations similar to those in adult blood erythrocytes. The remaining 70 to 85% have CA I concentrations which are 100-fold less and CA II concentrations which are 5- to 20-fold less than those found in adults. Thus, contrary to past reports, the magnitude of the developmental change in CA I concentration closely resembles the magnitude of change in HbA levels.
D000375:Aging; D002256:Carbonic Anhydrases / Q000097:blood* / Q000235:genetics; D004912:Erythrocytes / Q000201:enzymology*; D005312:Fetal Blood / Q000201:enzymology*; D005786:Gene Expression Regulation; D006441:Hemoglobin A / Q000235:genetics*; D006461:Hemolysis; D006801:Humans
D006441:Hemoglobin A; D002256:Carbonic Anhydrases