Effect of retinoic acid on the acylation of phospholipids in granulocytes in vitro.
The influence of retinoic acid on the incorporation of [1-14C]palmitic acid and [1-14C]arachidonic acid into phospholipids was examined in guinea pig peritoneal granulocytes. All-trans-retinoic acid inhibited the incorporation of both fatty acids into phosphatidic acid and phosphatidylinositol. However, it stimulated the incorporation of both fatty acids into phosphatidylcholine but not other phospholipids. All-trans-retinoic acid was more effective than 13-cis-retinoic acid. The influence of all-trans-retinoic acid on the acylation of phospholipids was concentration-dependent with significant effect occurring at 2.1 microM. The loss of labeled fatty acids from prelabeled phospholipids and the transport of labeled fatty acids into granulocytes were not responsive to the presence of retinoic acid in the incubation media. These results suggest that retinoic acid may affect the activities of acyltransferases involved in the synthesis of phosphatidic acid, phosphatidylinositol and phosphatidylcholine.
D000215:Acylation; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000097:blood*; D010743:Phospholipids / Q000097:blood*; D014212:Tretinoin / Q000494:pharmacology*
D001095:Arachidonic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D016718:Arachidonic Acid; D019308:Palmitic Acid; D014212:Tretinoin