Effect of glutathione and uridine-5'-diphosphoglucuronic acid on the mutagenicity of tryptophan pyrolysis products (Trp-P-1 and Trp-P-2) by rat-liver and -intestine S9 fraction.
In the Ames test, after the addition of glutathione (GSH) or uridine-5'-diphosphoglucuronic acid (UD-PGA), we observed for Trp-P-1 an unchanged or a reduced mutagenicity by both the liver and intestine S9 fraction. For Trp-P-2, the same was true when we used the intestine S9 fraction. In the presence of liver S9 fraction, Trp-P-2 mutagenicity was also decreased by the addition of UDPGA but was increased by the addition of GSH. These results show that cofactors for glucuronide and GSH conjugation may alter the metabolic activation of Trp-P-1 and Trp-P-2 and consequently their mutagenicity.
D000818:Animals; D002243:Carbolines / Q000633:toxicity*; D014453:Glucuronosyltransferase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D007211:Indoles / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism / Q000494:pharmacology*; D014539:Uridine Diphosphate Sugars / Q000494:pharmacology*
D002243:Carbolines; D007211:Indoles; D009153:Mutagens; D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D005978:Glutathione; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole