Immunonephelometry of apolipoprotein A-II in hyperlipoproteinemic serum.
Immunonephelometry of apolipoprotein A-II (apo A-II) in serum requires that factors responsible for nonspecific increases of light scattering be eliminated. Complete delipidation is not necessary for immunonephelometry of apo A-II; in fact, light scattering of immunocomplexes from delipidated HDL is greater than from isolated apo A-II. Therefore, using the isolated peptide as a standard does not suffice for measurements in serum. The concentration of protein influences the development of light scattering and must be considered in standardizing this measurement.
D016633:Apolipoprotein A-II; D001053:Apolipoproteins / Q000097:blood*; D001798:Blood Proteins; D003902:Detergents; D005260:Female; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood*; D005779:Immunodiffusion; D008075:Lipoproteins, HDL / Q000302:isolation & purification; D008297:Male; D009391:Nephelometry and Turbidimetry; D011863:Radioimmunoassay; D012016:Reference Values
D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D001798:Blood Proteins; D003902:Detergents; D008075:Lipoproteins, HDL