Establishment of asparaginase- and guinea pig complement-resistant subline of murine lymphoblastic leukemia L5178y cells.
Asparaginase-resistant subline L5178Y/Asp of murine lymphoblastic leukemia L5178Y cells was established from resistant clones isolated from soft agar cultures treated with Escherichia coli asparaginase. The L5178Y/Asp cells were found to be over 1000-fold more resistant to E. coli asparaginase, co-resistant to inhibitory effect of guinea pig serum (complement), more tumorigenic for syngeneic DBA/2 mice, and to grow much better (higher plating efficiency) in soft agar medium. With the use of the resistant subline in immunocytotoxicity-cytostasis test in the presence of guinea pig complement, we have been able to demonstrate the presence of tumor-associated antigen(s) on the L5178Y cells which is/are also demonstrable by enzyme-linked immunosorbent assay.
D000818:Animals; D001215:Asparaginase / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004261:DNA Replication / Q000187:drug effects; D004351:Drug Resistance; D004926:Escherichia coli / Q000201:enzymology; D006168:Guinea Pigs; D007940:Leukemia L5178 / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice
D003165:Complement System Proteins; D001215:Asparaginase