[Quantitative determination in the culture examination of the sputum].
1448 sputa specimens were examined by a quantitative research: they were fluidified with dithiothreitol and subsequently serially diluted; was also performed a microscopic examination in order to notice the presence of leukocytes. If we consider as significant a microbic concentration greater than or equal to 10(7)/ml, the 61.5% for specimens presented a saprophytic flora, the 15.1% was included from 10(5) to 10(6)/ml and the 23.3% had a concentration greater than 10(7)/ml. The sputa with a concentration greater than or equal to 10(7)/ml had a prevalence of Pneumococcus, Haemophilus and Pseudomonas, often in pure culture. The authors emphasize the importance of quantitative assay in sputum's culture and at the same time of cytologic examination.
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003470:Culture Media; D006190:Haemophilus / Q000302:isolation & purification; D006801:Humans; D011549:Pseudomonas / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000302:isolation & purification
D003470:Culture Media
Osservazioni sulla determinazione quantitativa nell'esame colturale dell'escreato.